LOCUS       DASWDC010000001         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1502519, whole genome shotgun sequence.
ACCESSION   DASWDC010000001 DASWDC010000000
VERSION     DASWDC010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502519"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VF995_00005"
     CDS             <1..457
                     /locus_tag="VF995_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9341983.1"
                     /translation="ITIAGMAGSVVLGAASDRIRRRKPFLVLAGVVAAPMLWLLGHLG
                     SLGALVPVAFVMGFFLLAALPIAIAVVSEDRTLGPQVGSTAVGVILLAGNLGSAVVVA
                     VMGALKGPGGDFSTAIALITGLAVVFVGITLTIPDPLRPGPAAAERAGP"
     gene            823..1242
                     /locus_tag="VF995_00010"
     CDS             823..1242
                     /locus_tag="VF995_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9341984.1"
                     /translation="MTRTIREVMTGNPRTISAGAPVTDAARAMRDADVGAVIVLDDGK
                     VGGIVTDRDITIRSTAFGYDPSSTPVRQVCSTDVTTLSPDDDLREAVLLMRSKNVRRL
                     PVVDGNQPVGMVSLGDLAIERDPESALSDISVAPPNQ"
     gene            complement(1328..2986)
                     /gene="ilvD"
                     /locus_tag="VF995_00015"
     CDS             complement(1328..2986)
                     /gene="ilvD"
                     /locus_tag="VF995_00015"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9341985.1"
                     /translation="MSNEPRFRPERAPARSHLRAMGLTDEEIARPVVGIASTWTGTMP
                     CNLNQRLLAEGVAAGVRAAGGTPLEFNTNAVSDNLTMGTEGMKASLVSREVIADSIEL
                     MVRAHRFDALVCLVGCDKTVPAAVMALCRLNLPAVVLYSGPMLPGSFRGRAVTIQDMW
                     EALGERAAGRFSDQDLAELERVACPGPGTCAGQYTANTMGTAIEFLGVAPFGSSLAPA
                     VDPARGEQAEQAGRLAMAVLAAGRRPLDLVTRVSLENAIAAVAATGGSTNGVLHLLAI
                     AREAGVPLTLDDFDAVSARTPVLASLKPGGEYVASDFHAAGGTPLLLAELVRGGLVHA
                     DARSVDGRSFGEIAGRVQGVPDGRVVATAERPFKPGGSLAILRGNLAPDGAVVKAGGT
                     TLRRHHGPARVFDSEEAAYMAVQQGGIAAGDVVVIRYEGPAGGPGMREMLDVTAAIVG
                     HGLDRDVALVTDGRFSGATRGLMVGHAAPEAAAGGPLAVLRDGDLIAIDVEARSLQVD
                     LSAEELRRRLDGWTAPPPRYPSGVFAKYVATVGSAADGAVTRVR"
     gene            complement(2983..>3384)
                     /locus_tag="VF995_00020"
     CDS             complement(2983..>3384)
                     /locus_tag="VF995_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9341986.1"
                     /translation="LLRGVFPDIPELVDLTFMLRYFPGAERVITTIAPRLDETVAVFA
                     GAGFSLEHVEGVTDIATASLRDAAARTRLRADTALQHLPDTEFEAGLRRLEADAAAED
                     PTQPPAPVYGRIPLVVFAKSTSESESEGAEG"
BASE COUNT          441 a         1264 c         1225 g          454 t
ORIGIN      
        1 gatcacgatc gccggcatgg ccggctcggt ggtcctgggc gcggccagcg accggatccg
       61 gcggcggaag ccgttcctgg tcctggccgg cgtggtcgcc gcgccgatgc tttggttgct
      121 ggggcacctc gggtcgctcg gcgcgctggt gccggtggcg ttcgtgatgg ggttcttcct
      181 gctggcggcc ctgccgatcg cgatcgcggt ggtgtcagag gaccgcacgc tcggcccgca
      241 ggtcggcagc accgccgtcg gcgtgatcct gctcgccggc aacctcggca gtgccgtggt
      301 ggtggcggtg atgggcgcgc tgaagggccc aggaggcgac ttcagcaccg ccatcgcgct
      361 catcaccggc ctggcggtgg tgttcgtggg gatcaccctc accatccccg acccgctgcg
      421 tccaggcccg gcggcggctg agcgcgccgg accctgaggc cggcaaggcc atcaccccag
      481 cgggatcacc acccgctggc agcgtggact gggtgtgcgg cttgccacgc ggggtggtgc
      541 accccgccgc cagggcgacg gcgagcagca ccaccaagcc aaggccggga actggaacgg
      601 ccctggggcg ttggccggtg tcggtcgggg ctgcgcgcat cctggtccag gatgacacgc
      661 ctccgctcga ccgcgccccg gtccccttgg gggtggcgtc gcggacgtgc tgggcgaacg
      721 accctacggt aagcctggcc gtgctggtgg tggtttggct tccccggccc ccggctacgc
      781 caggacttga ggaacacgac gatacggaag gagtgttcca ggatgactcg tacgatccgt
      841 gaggtcatga ccggcaaccc gcggaccatc agcgccggag cgccggtcac cgacgccgcc
      901 cgcgccatgc gggacgccga tgtcggcgcg gtgatcgtgc tggacgatgg caaggtcggc
      961 ggcatcgtca cggaccggga catcaccatc cggtcgaccg ccttcggcta cgacccgagc
     1021 agcacgcccg tccgccaggt gtgcagcacc gacgtgacca cgctgtcgcc cgacgacgac
     1081 ctgcgtgagg ccgtcctgct gatgcggtcc aagaacgtcc gccggctccc ggtcgtcgat
     1141 ggcaaccagc cagtcggcat ggtttcgctc ggcgatctcg ccatcgagcg cgaccctgag
     1201 tcggcgctca gcgacatcag cgtggcgccg cccaaccagt gatcagcgaa ccggccgccg
     1261 tcaccgacgg ccgccacgat ggcgccgcca cggcgccatc gtctccctct acctgaccct
     1321 ggtcagccta cctgaccctg gtcaccgcac cgtcggcggc ggagccgacc gtggcgacgt
     1381 acttggcgaa cacgccggag gggtagcgcg gcggcggcgc ggtccagccg tccaggcggc
     1441 ggcgcagctc ctcggcgctc aggtccacct ggagcgagcg ggcctccacg tcgatggcga
     1501 tcaggtcgcc gtcgcgcagg accgccaggg gaccgccggc ggctgcctcc ggcgcggcat
     1561 ggccgaccat cagcccacgg gtcgcgccgc tgaagcgccc gtcggtcacc agcgcgacgt
     1621 cgcggtcgag gccgtggccg acgatcgcgg cggtgacgtc gagcatctcc cgcattcccg
     1681 gcccgccggc tgggccctcg tagcggatca ccaccacgtc gccggcggcg atcccgccct
     1741 gctggacggc catgtaggcc gcctcctcgg agtcgaacac ccgcgcgggg ccgtggtggc
     1801 gccgcagcgt ggtccccccg gccttgacca ccgccccgtc gggggcgagg ttgccgcgca
     1861 ggatcgccag cgacccgccc ggcttgaagg gccgctcggc cgtggcgaca acgcggccgt
     1921 cgggcacgcc ttggacgcgg ccggcgatct cgccgaagct gcgcccatcc acgctgcgcg
     1981 cgtcggcgtg gaccaggccg ccgcgcacca gctcggccag cagcaggggc gtgccgccgg
     2041 cggcgtggaa gtcgctggcg acgtactccc cgcctggttt caggctggcg agcaccgggg
     2101 tgcgggcgct gaccgcgtcg aagtcgtcca gggtgagcgg cacgcccgcc tcccgcgcga
     2161 tcgccagcag gtgcagcacc ccgttggtgg agccgccagt cgccgccacc gcggcgatgg
     2221 cgttctccag cgatacccgg gtgaccaggt cgagcgggcg ccgcccggcg gccagcaccg
     2281 ccatggcgag gcggccggcc tgctcggcct gctcgccccg cgccgggtcc accgccggcg
     2341 cgaggctgga cccgaacggg gccaccccga ggaactcgat cgccgtgccc atggtgttgg
     2401 cggtgtactg cccggcgcag gtccccgggc cggggcaggc gacccgctcc agctcggcca
     2461 ggtcctggtc ggagaaccgc cccgccgccc gctctcccag cgcctcccac atgtcctgga
     2521 tggtgaccgc ccggccgcgg aagctccccg gcagcatcgg cccgctgtag agcaccaccg
     2581 cggggaggtt caggcggcac agcgccatca ccgccgccgg cacggtcttg tcgcagccga
     2641 ccaggcacac cagcgcgtcg aagcggtgcg cgcgcaccat cagctcgatc gagtcggcga
     2701 tcacctcccg gctgaccagc gacgccttca tcccctcggt gcccatggtg aggttgtccg
     2761 acaccgcgtt ggtgttgaac tccagcggcg tcccacccgc cgcccgcacc cccgccgcga
     2821 ccccctccgc cagcagccgc tggttcaggt tgcacggcat cgtccccgtc caggtcgagg
     2881 cgatccccac caccgggcgg gcgatctcct cgtcggtcaa ccccatcgcc cgcaggtgcg
     2941 agcgggcagg cgcccgctca ggccggaacc gcggctcgtt gctcatcctt cagctccctc
     3001 ggactcggac tcggacgtgg acttggcgaa cacgaccagg gggatgcggc cgtagaccgg
     3061 cgcgggcggc tgggtggggt cctcggcggc ggcgtcggcc tccaggcggc ggagccccgc
     3121 ctcgaattcc gtgtcgggca ggtgctgcag tgcggtgtcg gcgcgcaggc gcgtgcgggc
     3181 ggcagcgtcg cggaggctgg ccgtggcgat gtcggtgacg ccctcgacat gctccagcga
     3241 gaacccggcg ccggcgaaga ccgccacggt ctcgtccagg cgtggcgcga tcgtggtgat
     3301 cacccgctcg gcccctggga agtagcgcag catgaaggtc aggtcgacca gctccgggat
     3361 gtcggggaag acgccgcgga gcag
//
LOCUS       DASWDC010000002         8239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_250558, whole genome shotgun sequence.
ACCESSION   DASWDC010000002 DASWDC010000000
VERSION     DASWDC010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250558"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VF995_00025"
     CDS             <1..452
                     /locus_tag="VF995_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9341987.1"
                     /translation="EKAQLAKQAGADHLATYEDFGEVVKQVTGGAGAAAVYDGVGAAT
                     FDASLDALRTRGSMILYGAASGPVPPFELRRLMTGGSLYIQRPTLVHFVATRDELLRR
                     SSDLFGWIRDSRLSVRIGGTYPLEEAAQAHEDLAGRRTTGKLLLLPR"
     gene            complement(469..867)
                     /locus_tag="VF995_00030"
     CDS             complement(469..867)
                     /locus_tag="VF995_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9341988.1"
                     /translation="MTLVVDASLVVAAFAGVSPLGSWADSVLDTNELLSAPHLMPVEA
                     ANILRREALLGHISADAASIAHADVTALPVELFPYVPLAARIWELRPNLTAYDAWYVA
                     LAEYLDARLATLDLKLAKAHGPRCGFVTPP"
     gene            complement(864..1100)
                     /locus_tag="VF995_00035"
     CDS             complement(864..1100)
                     /locus_tag="VF995_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9341989.1"
                     /translation="MTTITIRNVPDEVRDELAARAAATGRSLQEYLRIRLIQMTQQLD
                     AEAWVAQVRAHKAATGGGTLTAEDILASRDADRR"
     gene            complement(1144..2229)
                     /locus_tag="VF995_00040"
     CDS             complement(1144..2229)
                     /locus_tag="VF995_00040"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009713239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HEX9341990.1"
                     /translation="MPHLVRGVVSRSKGAPVEVTTVSVPDPGPGEALVRVQACGVCHT
                     DLHYREGAITSDFPFLLGREAAGVVEAVGPDVTNLTPGDFVVLAWRAPCGHCRSCRRG
                     RPWYCFDSRNATQKMTLEDGTELRAALGIGAFAEKTLVAAGQAVKVDPAARPEAAGLV
                     GCGVMAGFGAALNTGGVQRGDTVAVFGCGGVGDAAIHAAALVGARKIIAVDVDERKLK
                     WAREFGATDIVDSSQTNPVDEIRRLTDGFGADVTIEAVGRPEVFKQAFHARDHAGVMV
                     QVGVPDPSMRIDLGMIDFFGRGGALKPSWYGDCLPTRDFPILIDLYLRGRYDLDRFVS
                     ETIPLDQVEEAFAKMQRGEVLRSVVVL"
     gene            2292..3626
                     /locus_tag="VF995_00045"
     CDS             2292..3626
                     /locus_tag="VF995_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9341991.1"
                     /translation="MEPIQESRSATLRRALVDELRRDGLLGTAGTGDGSAGGGGPARL
                     AEAFLAVPRERFLPEHAARDGLEAVYANRAIPTKLGRNGGPVSSSSQPSIMALMLGLL
                     DLRPGQGVLEVGAGTGYNAAMIGQLVGPGGRVVSVEIDPELAARASAALAAGGHHAAV
                     AVGDGRDGWPAGAPYDRIIVTASSPDLPKAWLDQLVEGGRIVVPLQLQHDTSDHFVQA
                     VCALRRTGDRFVPAGTVDGSFMPLRPRADAETPYPAGIGASEHHDGNSALLSQLSGAA
                     LRSLPQAARRKLLALTLSPPRSSRLTRPAPCTALELFLVLAAPPDRLVLHYGNGTKAI
                     GVIDRGGTGLAVVGGRGGRASRLLAWGDPEPEVLLRSLIARWRALGRPGAENLLIGAS
                     YPASRRLGRAPPGGGRPPAEHHWRTLRRPNCILELDWLPLGDADSADKPVPD"
     gene            complement(3639..7106)
                     /gene="dnaE"
                     /locus_tag="VF995_00050"
     CDS             complement(3639..7106)
                     /gene="dnaE"
                     /locus_tag="VF995_00050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9341992.1"
                     /translation="MPDSFTHLHVHTEYSMLDGAARVNDLFAEAARMGMPALAMTDHG
                     SLFGAVDFYEAGKKHGVKPIIGMEAYLAPDSRHRKQKAHAGEEPYSHLTLLAETQQGY
                     HNLLRLVSSASLEGYFYKPRMDRELLDRHHDGIVGTSGCLGGEVSQALLKGERDKARA
                     TLAEFRDIFGAGNYFVEVMDHGLPEDKRVNPELIALARELDLPLLATNDLHYTHKRDH
                     GPHEVLLCIQTAATLADPKRFKFEAEEFYLKSPAEMRALFPNDEAPGACDNTLAIAER
                     CNVELQFGTNILPRFDVPDGFDLNSWLRHEVLAGAAERYGDPLPKEAAERIDYELSII
                     GQLGFAGYFLIVRDLCDHAREHGIRVGPGRGSAAGSCVSYCLGITDLDPIYYRLMFER
                     FLNPERREMPDIDLDFDERRRAEMIRYATEKYGDDHVAQIVTFSTIKAKQAIRDSARV
                     LGFPYKLGDDLAKMMPPPVLGKEFPLAEAYKLSAELRGAKETDPDARRVLETAEGLEG
                     LRRQWGVHAAAVVIARDPLVETVPIMRREADGAIITQYEMNGVAKLGLLKMDFLGLRN
                     LTVLDDTLKHLNARGVELDLGTLPTDDADTFKMLCQGDTDGVFQMESEGVRRILRQLR
                     PDRFEDVVAVIALYRPGPMEQIPIYIQGKQDPRKITYLHPLMEELTADTYGVMVYQEQ
                     IIMLLQKVAGYTAGEADIVRKAIGKKKLDLMKQEEPRFIAGCQDKGLSKKDAETLWAL
                     IQPFAGYSFNRAHAAGYGLVTWQTAYLKAHYPVEYMAALLTSVKDDKDARPKYLGSCR
                     RSGITVLPPDVNDSDSDFTPARTDPELADPDGIRYGLSAVRNVGGQVVEGIIAARRSK
                     GRFTDFFDFCDKVDAGVLNKRVVESLIKAGAFDSFGHSRKGLLEIHEDHVEAMVARKR
                     AEAAGQFSLFGGMDSGASAAVAEPVPTIRPEDWDKIQRLAFEKEMLGQYVSDHPLLGM
                     EAVLARSADTRLSVLAERADGATVMVAGILAKLIKKFTKRGETYVVAEMEDLDGAAEC
                     IFFPQMYQQHQGLLVQDSVVVVKARVDARDETPKLVAMDVKPPKLDDPGTSPVELVVE
                     AHRCDDGMVDRLKRILGSHRGKAPVLLQIRGAHHTMVVRLGDEFSVDPRNGLYAEIKA
                     ELGPDALLA"
     gene            complement(7275..>8239)
                     /locus_tag="VF995_00055"
     CDS             complement(7275..>8239)
                     /locus_tag="VF995_00055"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX9341993.1"
                     /translation="GPGPGPEHTHTATVPEERAGQRLDEVVAALAGVSRASVARWAAA
                     GQVLVEGRARPKSYRLRGGEQLAWEPPAAPPAEAPQPEALPLHVRYEDEHLLVVAKPA
                     GLVVHPAPGHPSGTLVNALLGREGTRLSAGGGGAGGGDRPGIVHRLDKDTSGLLLVAK
                     DDTTHAALSRELAAHRIGRRYLALAQGHLAPTGTVDAPLGRHPRDRKRMAVVPESSGR
                     RAVTHWQVQESFPAVDLLEVALETGRTHQIRVHLAHLRHPVAGDRTYGADPVLAARLG
                     LARPFLHAWRLAFAHPVTGEPVEITEALPADLERVLQRLRQENF"
BASE COUNT         1153 a         2787 c         3050 g         1249 t
ORIGIN      
        1 ccgagaaggc ccagctcgcc aagcaggccg gcgcggatca tctggccacg tacgaggact
       61 tcggcgaggt ggtcaagcag gtgaccggcg gtgccggcgc ggcagcggtc tacgacggcg
      121 tcggcgcggc gaccttcgac gcgagcctgg acgccctgcg gactcgcggc tccatgatcc
      181 tgtacggggc ggccagcggg ccggtccccc ccttcgagct ccgccgcctg atgaccggcg
      241 gttcgctgta catccagcgc ccgaccctgg tccatttcgt cgccacccgc gacgagctgc
      301 tgcgacgcag cagcgacctg ttcggctgga tccgcgacag ccgcctgtcg gtccgcatcg
      361 gcggcaccta tccgctggag gaggccgccc aggcccacga ggacctggcc gggcgacgga
      421 ccaccggcaa gctgctcctg ctgccccgct gacccctggc gggcctgatc agggaggggt
      481 cacgaagccg cagcgcggcc catgcgcctt ggccagcttg aggtcgagcg ttgcgagtct
      541 cgcgtcgaga tattccgcga gcgcgacata ccaggcgtcg taggctgtga ggttggggcg
      601 cagctcccag atccgtgcgg ccaggggcac atacggaaat agctcgactg gcagggccgt
      661 cacatcggca tgggcgatcg aggcggcgtc cgccgaaatg tgtccgagca gagcctcgcg
      721 ccggaggatg ttggcagcct ccacaggcat caagtgcggc gcgctcaaca gctcgttggt
      781 atcgagcacc gagtcagccc aactgccgag tggactgaca ccagcgaacg cggccaccac
      841 caatgaggca tccacgacca gggtcatcga cgatcagcgt cgcggctggc gaggatatcc
      901 tcggctgtca gtgtcccccc acccgtcgcg gccttgtggg cacgcacctg cgcgacccag
      961 gcctcggcat caagttgctg ggtcatctgg atcaaccgga ttcggaggta ctcctgtagc
     1021 gaacggccag tcgccgccgc gcgagcagca agctcgtcgc ggacttcgtc aggcacgttc
     1081 cgaatcgtga tcgttgtcat agatgcattt tgcagctagt gacgggcaca tcgcaagcac
     1141 gaatcaaagg acgacgacgc tgcggaggac ctcaccgcgc tgcatcttgg cgaaggcctc
     1201 ctcgacctgg tcgagcggga tcgtctcgga gacgaaacgg tcgaggtcgt agcggccccg
     1261 caggtacagg tcgatcagga tcgggaagtc ccgggtcggc aggcagtcgc cataccagga
     1321 gggcttcagg gcgccgccgc ggccgaagaa gtcgatcatg ccgaggtcga tccgcatcga
     1381 cgggtcgggg acgccgacct ggaccatgac gccggcgtgg tcgcgggcgt gaaaggcctg
     1441 cttgaagacc tcggggcgac ccacggcctc gatggtgacg tcggcgccga agccgtcggt
     1501 gaggcggcgg atctcgtcga ccgggttggt ctgcgacgag tcgacgatgt cggtggcgcc
     1561 gaactccctg gcccacttga gcttccgctc gtcgacgtcg acggcgatga tcttgcgggc
     1621 gccgaccagg gcggcggcgt ggatggcggc gtcgccgacc ccgccgcagc cgaacaccgc
     1681 gacggtgtcg ccgcgctgga cgcctccggt gttgagggcg gcgccgaagc cggccatgac
     1741 gccgcagccg accagcccgg cggcctccgg ccgggcggcg gggtcgacct tgaccgcctg
     1801 gccggcggcg accagggtct tctcggcgaa cgcgccgatc ccgagcgcgg cacgcagctc
     1861 ggtgccgtcc tccagggtca tcttctgggt cgcgttgcgg gagtcgaagc agtaccacgg
     1921 ccgcccgcgg cggcaggagc ggcagtgccc gcagggggcc cgccaggcca gcaccacgaa
     1981 atcgccgggc gtgaggttgg tgacgtccgg gccgacggcc tcgaccaccc cggcggcctc
     2041 gcggccgagc aggaagggga agtccgaggt gatcgcccct tcgcggtagt gcaggtcggt
     2101 gtggcagacc ccgcaggcct gcacccggac gagcgcctca ccaggaccgg gatcgggcac
     2161 cgagaccgtg gtgacctcca ccggagcgcc cttcgaccgt gacacgaccc cacggaccag
     2221 gtgtggcatt gtgaactcct ctcgcttcca ctgctcacca aagcgatacg gtactgcagg
     2281 ggttcaccga catggagccg attcaggaga gcaggagcgc caccctgcgc cgcgcgctgg
     2341 tcgacgagct gcgccgcgac ggcctgctcg gcactgccgg caccggggac ggatccgcgg
     2401 ggggaggcgg cccggccagg ttggccgagg cgttcctggc ggtcccccgg gagcggttcc
     2461 tgccggagca tgccgcgcgg gacgggctgg aagcggtcta cgccaaccgg gcgatcccca
     2521 ccaagctcgg ccgcaacggc gggccggtca gctcctcctc gcagccgtcg atcatggcgc
     2581 tgatgctcgg cctgctcgac ctgcggcctg gccagggggt gctggaggtc ggcgccggga
     2641 ccggctacaa cgccgccatg atcggccagc tggtcgggcc tggcgggcgg gtggtcagcg
     2701 tcgagatcga cccggagctg gccgcccgcg ccagcgcggc gctcgccgcc ggcggccacc
     2761 acgccgcggt cgcggtcggc gacggccgcg acggctggcc ggcgggcgcg ccctacgacc
     2821 ggatcatcgt gaccgccagc tcccccgacc tgccgaaggc ctggctcgac cagctggtgg
     2881 agggcggccg gatcgtggtg ccgctgcagc tccagcacga caccagcgac catttcgtgc
     2941 aggcggtgtg cgcgctgcgc cggaccggtg accgcttcgt gccggccggc acggtcgacg
     3001 gcagcttcat gccgctgcgg ccccgcgccg acgccgagac cccctacccc gccgggatcg
     3061 gcgcgagcga gcatcacgat ggcaactccg ccctgctgag ccagctctcc ggcgcggcgc
     3121 tgcggagcct cccgcaggcg gcacggcgca agctgctggc gctgaccctc tccccgccgc
     3181 ggtccagccg gctcacccgg ccagcgccct gcaccgcgct ggagctgttc ctggtgctgg
     3241 cggcaccgcc cgaccggctg gtgctgcact acggcaacgg caccaaggct atcggcgtga
     3301 tcgaccgggg cggcaccggc ctcgcggtgg tcggcggtcg gggcggccgc gcgagccgcc
     3361 tgctggcctg gggcgacccc gagccggagg tgctcctgcg gtcgctgatc gccaggtggc
     3421 gcgccctcgg ccgccccggc gcggagaacc tgctgatcgg ggcgagttac cccgcctcgc
     3481 gccgcctcgg ccgggccccg ccgggcggcg ggcgccctcc agccgaacac cactggcgca
     3541 ccctgcggcg tccgaactgc atcctggagc tggactggct gccgctgggc gacgccgaca
     3601 gtgcggacaa accggtccca gattgaggcg gtccgccctc aggcaagcag cgcgtccggg
     3661 ccgagttcgg ccttgatctc ggcgtagagg ccgttgcggg ggtcgacgct gaactcgtcg
     3721 ccgaggcgga cgaccatggt gtggtgggcg ccgcggatct gcagcaggac gggggctttg
     3781 ccgcggtggg agccgaggat gcgcttgagg cggtcgacca tgccgtcgtc gcaacggtgg
     3841 gcctcgacga ccagctcgac cgggctggtg ccggggtcgt cgagctttgg gggcttcaca
     3901 tccatggcga ccagcttcgg ggtctcgtcg cgggcgtcca cccgagcctt gaccaccacc
     3961 accgagtcct ggaccagcag cccctgatgc tgctggtaca tctgcgggaa gaagatgcat
     4021 tcggcggcgc cgtcgaggtc ctccatctcg gcgaccacgt aggtctcgcc gcgcttggtg
     4081 aacttcttga tcagcttggc gaggatcccg gcgaccatca cggtggcgcc gtcggcgcgt
     4141 tcggccagca ccgacaggcg cgtgtcggcc gagcgggcca gcaccgcctc catgccgagc
     4201 agggggtggt cggagacgta ctggccgagc atctccttct cgaaggcgag ccgctggatc
     4261 ttgtcccagt cctcggggcg gatggtcggg accggctcgg ccacggccgc gctggccccg
     4321 ctgtccatgc cgccgaacag cgagaactgc ccggcggcct cggcacgctt gcgggcgacc
     4381 atcgcctcga cgtggtcttc gtggatctcc agcaggccct tgcgggaatg gccgaaggag
     4441 tcgaaggcac ccgccttgat cagcgactcg accacccgct tgttgagcac cccggcgtcg
     4501 accttgtcgc agaagtcgaa gaagtcggtg aagcggccct tggaacggcg ggcggcgatg
     4561 atcccctcga cgacctgccc gccgacgttg cgcaccgccg agaggccgta gcggatcccg
     4621 tccgggtcgg ccagctcggg atcggtccgc gccggggtga agtcggagtc ggagtcgttg
     4681 acgtccgggg gcagcacggt gatccccgag cggcggcagg acccgaggta cttcggccgc
     4741 gcgtccttgt cgtccttgac gctggtgagc agcgccgcca tgtactcgac cgggtagtgg
     4801 gccttgaggt aggcggtctg ccaggtgacc aggccgtagc cggcggcgtg ggcgcggttg
     4861 aaggagtagc ccgcgaacgg ctggatcagc gcccacagcg tctcggcgtc cttcttgctg
     4921 aggcctttgt cctggcagcc ggcgatgaag cggggctctt cctgcttcat caggtcgagc
     4981 ttcttcttgc cgatcgcctt gcggacgatg tcggcctcgc cggcggtgta gccggcgacc
     5041 ttctgcagca gcatgatgat ctgttcctgg tagaccatca cgccgtaggt gtcggcggtc
     5101 agctcctcca tcagcgggtg caggtaggtg atcttgcgcg ggtcctgctt accctggatg
     5161 tagatgggga tctgttccat cggccccggg cggtacaggg cgatgaccgc gaccacgtcc
     5221 tcgaaccggt cggggcgcag ctgccgcagg atccgccgga cgccttccga ttccatctgg
     5281 aacaccccgt cggtgtcccc ctggcacagc atcttgaagg tgtcggcgtc gtcggtcggc
     5341 agcgtgccga ggtccagctc gaccccgcgc gcgttcaggt gcttgagggt gtcgtccagc
     5401 accgtgaggt tgcgcaggcc caggaagtcc atcttgagga ggccgagctt ggccaccccg
     5461 ttcatctcgt actgggtgat gatcgcgccg tcggcctctc gccgcatgat cggcacggtc
     5521 tcgaccagcg ggtcgcgggc gatcaccacc gcggcggcgt gcacgcccca ctggcgccgc
     5581 agcccctcaa ggccctcggc ggtctccagc acccggcggg cgtcggggtc ggtctccttg
     5641 gcgccgcgca gctcggccga cagcttgtag gcctcggcca gcgggaactc cttgccgagg
     5701 accggcgggg gcatcatctt ggcgaggtcg tcgccgagct tgtaggggaa gccgagcacc
     5761 cgggccgagt cgcggatcgc ctgcttggcc ttgatggtgg agaaggtgac gatctgcgcg
     5821 acgtggtcgt cgccgtactt ctcggtggcg tagcggatca tctcggcgcg gcgacgttcg
     5881 tcgaagtcca ggtcgatgtc gggcatctcg cggcgctcgg ggttgaggaa gcgctcgaac
     5941 atcaaccggt agtagatcgg gtcgaggtcg gtgatcccca ggcagtagga gacacaggag
     6001 ccggccgcgc tgccccggcc cggcccgacg cggatgccgt gctcgcgggc gtggtcgcag
     6061 aggtcgcgga cgatcaggaa gtagccggcg aagccgagct ggccgatgat cgacagttcg
     6121 tagtcgatcc gctcggcggc ctccttcggc agcgggtcac cgtagcgctc ggcggcgccg
     6181 gcgagcacct cgtgccgcag ccaggagttg aggtcgaagc cgtcggggac gtcgaagcgc
     6241 ggcaggatgt tggtgccgaa ctgcagctcg acgttgcagc gctcggcgat cgccagggtg
     6301 ttgtcgcagg caccgggcgc ctcgtcgttg gggaacaggg cgcgcatctc ggccggggat
     6361 ttgaggtaga actcctcggc ctcgaacttg aagcgcttgg ggtcggcgag ggtggcggcc
     6421 gtctggatgc agagcagcac ctcgtgcggg ccgtggtcgc gcttgtgggt gtagtgcagg
     6481 tcgttggtcg cgagcagggg aaggtccagc tcgcgcgcca gcgcgatcag ctccgggttg
     6541 acccgcttgt cctcgggcag gccgtggtcc atgacctcga cgaagtagtt gccggcgccg
     6601 aagatgtcgc ggaactcggc gagggtggcc cgcgccttgt cgcgttcgcc cttgagcagc
     6661 gcctggctga cctcgccgcc gaggcagccg gaggtgccga cgatgccgtc gtggtggcgg
     6721 tcgagcagct cgcggtccat ccgcggcttg tagaagtacc cctccagcga cgcggacgac
     6781 accagccgca gcaggttgtg gtagccctgc tgcgtctcgg ccagcagcgt caggtggctg
     6841 tagggttcct cgccggcatg cgccttctgc ttgcggtgcc gcgagtcggg ggctaagtag
     6901 gcttccatgc cgataatcgg cttgacgccg tgcttcttgc cggcctcgta gaagtcgacg
     6961 gcgccgaaga gggagccgtg gtcggtcatg gcgagggccg gcatgcccat ccgggccgcc
     7021 tcggcgaaca ggtcgttcac ccgggcggcg ccgtccagca tcgagtactc ggtgtggacg
     7081 tgcagatggg tgaagctgtc tggcacctct ccccctcgct ccgtccacag gctgtgggta
     7141 atgtttccac agggtgtgga caaattacat ggctgtaatc cagccgcagg ataccccggc
     7201 cccgaccccg tgggcaatgg cgtccggatc gctgaatccc ttgcggcaca aggggttcag
     7261 cgagaagccg caggtcagaa attttcttgg cggaggcgct ggagtacccg ttcgaggtcc
     7321 gctgggaggg cttcggtaat ttcgactggt tcgcccgtca ccggatgcgc aaaggcgagc
     7381 cgccaggcgt ggaggaacgg ccgcgccagc ccgaggcgcg cggcgagcac cgggtcggcg
     7441 ccgtaggtgc ggtcccccgc caccgggtgg cgcaggtggg ccaggtgcac gcggatctgg
     7501 tgggtgcgcc cggtctccag cgcgacctcc agcaggtcga cggcggggaa gctctcctgg
     7561 acctgccagt gggtgacggc gcggcgcccg ctcgactcgg ggacgacggc catgcgcttg
     7621 cggtcgcggg ggtggcggcc gagcggcgcg tcgacggtgc cggtgggcgc caggtggcct
     7681 tgggcgaggg cgaggtagcg gcgcccgatg cggtgggcgg ccagctcgcg gctgagggcg
     7741 gcgtgggtgg tgtcgtcttt ggcgaccagc agcagcccgg aggtgtcctt gtcgaggcgg
     7801 tggacgatcc cgggccggtc gccgccaccc gccccgccgc cgccggccga caggcgggtg
     7861 ccttcgcggc cgagcagcgc gttgaccagc gtgccgctgg ggtggccggg ggcggggtgg
     7921 acgaccagcc cggcgggctt ggcgaccacc agcaggtgct cgtcctcgta gcggacgtga
     7981 agcggcagcg cctccggctg gggcgcttcg gctggagggg cggcgggggg ttcccaggcg
     8041 agctgctcgc cgccgcgcag acggtacgac ttcgggcgag ccctgccctc caccaggacc
     8101 tgcccggcgg ccgcccagcg cgccacggac gcacgggaga ccccggcgag cgcggcgacc
     8161 acctcgtcga ggcgctggcc cgcgcgctcc tcggggacgg tcgcggtgtg ggtgtgctcg
     8221 ggtccgggtc caggtccag
//
LOCUS       DASWDC010000003         3070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1335750, whole genome shotgun sequence.
ACCESSION   DASWDC010000003 DASWDC010000000
VERSION     DASWDC010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335750"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VF995_00060"
     CDS             complement(<1..712)
                     /locus_tag="VF995_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9341994.1"
                     /translation="MRCQEERMAELWYPSASRSAIAPVDAGSFTGGGHKGLFHTTEGA
                     TAAGAIAAFRSTGSWPHFTGSFEGGRFRIWQHIPINRAARSLVHLAGHVETNRNNVIQ
                     IELVGTAAHPPSAPGYLAGIATWMQWVEANFGVPHRSTVTFKAYPGSYGTNNGVRLSE
                     SSWNAYFGWLGHEHVPENLHGDPGALNIHALLQEDEDVPFTSQQLIEYAYEGARKALN
                     EGTAFGTKSWAETSKGTLA"
     gene            complement(809..2197)
                     /locus_tag="VF995_00065"
     CDS             complement(809..2197)
                     /locus_tag="VF995_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX9341995.1"
                     /translation="MNPRASAAARRTTVSGPPMTGAEVRQRDGGAGAPVPGPPSSTQA
                     HATGQGRRAAPRRLLPWLGLLGPGLIASSAGNDAGGIATYSSAGSQFAYRMLFVLLLV
                     AVGLALVQEMAARLGAYTGKGLAALIREEFSLRVAGFALICLLVANLGLVVSEFAGIG
                     AALELFGVSRYLSVPIAALGVWGLVVLGSYRYAERLFLLMALVFFTYPVAAVMAHPNW
                     HTVAADTFWPHLLASRHFLFLGVAIVGTTISPYMQLYQAAAVADKGIGPQDYKLERID
                     AVGGAIFACLIAFFIMVATAATIGGTGALHSAAEAAHALRPVAGANAEVLFGIGLLGA
                     SALAAAVVPLSTAYALSEAIGVERSVSRTFTQAPLFLGLFSGQIAVGAAIALLPGNLI
                     SLLINAYVVNGLITPIILVFILVLTSRRQLLGPAANTPAFRTLASGVVAVVSLLSVVV
                     LAQTVLGWLGLD"
     gene            complement(2194..>3070)
                     /locus_tag="VF995_00070"
     CDS             complement(2194..>3070)
                     /locus_tag="VF995_00070"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9341996.1"
                     /translation="GVDVSLQTLLRRLGPARFRSLPTPDRVIDWAAIQPFAGDEGQVP
                     LRTWKQGLQRLRPGELADLLEDLGRSARQELLAALEPDTAADALEEMEPRELEALLHE
                     APPARAAALVASMEPDEAVDALRGLGASERAELLDRMPPATARQLAGLLDFPSDCAGG
                     FMTTSLVRVSRFETVAEVRERLRTLAERQPDLDTVAVVDERGRLVDVVSVLELFVADP
                     GERVAVLTRAPAAVAISADARLEQVVEGLTEARESTLVVVDDLGRPLGRILADDVIDA
                     LLPERGRLRFPRLLR"
BASE COUNT          476 a         1015 c         1179 g          400 t
ORIGIN      
        1 tggcgagggt ccccttgctg gtctccgccc agctcttggt cccgaaagcg gtgccctcgt
       61 tgagggcctt gcgagcgccc tcgtaggcgt actcgatgag ctgctggctg gtgaacggca
      121 cgtcctcatc ctcctgcaga agtgcgtgga tattcagcgc ccccggatca ccgtggaggt
      181 tttcgggcac gtgttcatgg ccaagccacc caaaatacgc attccatgac gattcagaca
      241 agcgaacgcc gttgttggtg ccgtacgaac ccggataggc cttgaaggtg acggtggagc
      301 ggtgggggac gccgaagttg gcttcgaccc attgcatcca ggtggcgatg ccggcgaggt
      361 agccgggggc ggacgggggg tgggcggcgg tgcccaccag ctcgatctgg atgacgttgt
      421 tgcggttggt ctccacgtgg ccggcgaggt ggaccaggga gcgggcggcg cgattgatgg
      481 ggatgtgctg ccagatccga aaccggccgc cctcgaagct gccggtgaaa tggggccatg
      541 agcccgtgct gcggaaggcg gcgatcgctc cggcggcggt cgcgccctca gtggtgtgga
      601 agagcccctt gtggccgccg ccggtgaagc taccggcgtc gaccggggcg atcgccgacc
      661 ggctcgccga cgggtaccac aattcggcca tgcgttcctc ctggcagcgc atcgggacgg
      721 gtgcgcttgc cctggcgaaa tctcctacga cgaggatcag aacgcaacct ctcataccca
      781 ccgtgagagg aggattaccg caatcaattc aatcgagtcc gagccagccg agcacggtct
      841 gtgccagcac aaccacggac agcaggctga cgacggcgac gacgccgctg gccagggtgc
      901 ggaacgccgg ggtgttggcg gcggggccga ggagctggcg gcggctggtg agtaccagga
      961 tgaagaccag gatgatgggg gtgatcaggc cgttgaccac gtaggcgttg atcagcaggc
     1021 tgatcaggtt gccgggcagc agcgcgatcg ccgcgccgac ggcgatctgg ccgctgaaca
     1081 gcccgaggaa cagcggcgcc tgggtgaagg tgcgcgacac cgaacgctcc accccgatcg
     1141 cctcggagag cgcgtaggcg gtcgacagcg gcaccaccgc ggcggccagc gccgaggcgc
     1201 cgagcaggcc gatgccgaac agcacctcgg cgttggcgcc ggcgaccggg cgcagggcgt
     1261 gggcggcttc ggcggcggag tggagcgcgc cggtcccgcc gatggtggcg gcggtggcga
     1321 ccatgatgaa gaaggcgatc aggcaggcga agatcgcccc gccgaccgcg tcgatccgct
     1381 ccagcttgta gtcctgcggg ccgatgccct tgtcggccac ggcggcggcc tggtagagct
     1441 gcatgtaggg cgagatggtg gtgccgacga tcgccacccc gaggaacagg aagtgccgcg
     1501 aggcgagcag gtgcggccag aaggtgtcgg ccgcgacggt gtgccagttg gggtgggcca
     1561 tgacggcggc caccgggtag gtgaagaaca ccagcgccat cagcaggaac agccgctcgg
     1621 cgtagcggta ggagccgagc accaccagcc cccagacccc gagcgcggcg atcgggaccg
     1681 acaggtagcg cgagaccccg aacagctcca gggccgcgcc gatgccggcg aactcgctca
     1741 ccaccaggcc gaggttggcg accagcaggc agatcagcgc gaagccggcg acccgcaggg
     1801 agaactcctc gcggatcagc gccgccagcc ccttgccggt gtaggcgccg aggcgggcgg
     1861 ccatctcctg gaccagcgcg agcccgaccg cgaccagcag cagcacgaac agcatccggt
     1921 aggcgaactg ggagccggcc gaggagtagg tggcgatccc tccggcgtcg ttgccggcgc
     1981 tggaggcgat caacccgggc ccgagcaggc cgagccacgg cagcagccgc cggggtgccg
     2041 ccctccggcc ctgccccgtg gcgtgggctt gcgtgctgga gggcggaccg ggcaccggcg
     2101 ccccggcccc gccgtcccgc tgcctgacct cggcgccggt catgggcggg ccgctgaccg
     2161 tcgtgcgccg cgctgccgcg ctcgccctgg ggttcaccgc agcaacctcg ggaagcggag
     2221 gcggccgcgt tcgggcagga gggcgtcgat gacgtcgtcg gcgaggatgc gaccgagggg
     2281 gcggccgagg tcgtcgacca ccaccagggt gctctcacgg gcttcggtga ggccttcgac
     2341 gacctgttcg agcctggcgt cggcgctgat ggcgaccgcg gcgggcgccc gggtcaggac
     2401 ggcgacccgc tcgccggggt cggcgacgaa caactccagg acgctgacca cgtcgaccag
     2461 gcggccgcgc tcgtcgacca cggcgacggt gtccaggtcg ggctggcgct cggccagggt
     2521 acggagccgc tcgcggacct cggccacggt ctcgaagcgg ctgacccgca cgaggctggt
     2581 ggtcatgaag ccgccggcgc agtcggaggg gaagtcgagc aggccggcga gctggcgggc
     2641 ggtcgcgggc ggcatgcggt cgagcagctc cgcacgttcg ctggcgccca gcccgcgcag
     2701 cgcgtcgacc gcctcgtcgg gttccatgct ggccaccagg gccgccgccc tggcaggggg
     2761 cgcttcgtgg aggagcgcct ccagctcgcg gggttccatc tcttccagcg cgtcggcggc
     2821 ggtgtcaggc tccaatgcgg cgagcagctc ctggcgggcc gagcggccca ggtcctccaa
     2881 aaggtcggcc agctcgccgg ggcggagccg ctgcaggccc tgcttccagg tgcgcagggg
     2941 gacctggccc tcgtcgccgg cgaacggctg gatcgcggcc cagtcgatca cccggtcggg
     3001 ggtgggcagc gaccggaagc gggccgggcc gaggcgccgc agcagcgtct gcaggctgac
     3061 gtcgacgccg
//
LOCUS       DASWDC010000004         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1335761, whole genome shotgun sequence.
ACCESSION   DASWDC010000004 DASWDC010000000
VERSION     DASWDC010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335761"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..635)
                     /locus_tag="VF995_00075"
     CDS             complement(231..635)
                     /locus_tag="VF995_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012185051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9341997.1"
                     /translation="MSSASLSTAGVLLIAVVAIEYGGISLLRFITRRIPGYLDNPLRQ
                     NLFRAGHAHAGVLVILALVGMLYVDQTGLSGTAKELIRWGLTAPPILMPLGFFLSVAS
                     PRAVRPNALISLVYLGAAVLAAGVLALGINLL"
     gene            825..1358
                     /locus_tag="VF995_00080"
     CDS             825..1358
                     /locus_tag="VF995_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9341998.1"
                     /translation="MQAALDAIVEGGPANLSLRDLARRAGVSHAAPAHHFGDKAGLLS
                     AIAAEGFERLAVTLDQAWRQTGSFLEVGVAYVRFAASHRAHFEVMFRPELYRPDDPAV
                     ARARAAARSLLYGPLADSSGADPRAAVAAWSLVHGLATLWLNRNLPPQLGDDPEQLAR
                     EVAGYLFRDRDRPPRAR"
     gene            complement(1378..1800)
                     /locus_tag="VF995_00085"
     CDS             complement(1378..1800)
                     /locus_tag="VF995_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9341999.1"
                     /translation="MGGSLPSPEHDDVHQRIHALHARVNQAWHQHAGTGQEPGWRDFH
                     QDYADLLAAIGDHAQEHVRSLAQTAARDPAGSLGLAAAPAAPAARVRPAPGPLANLRS
                     GPGRVGPDGRSRQLPALGRPRTAAATTSASPAWQALDL"
     gene            complement(1851..2066)
                     /locus_tag="VF995_00090"
     CDS             complement(1851..2066)
                     /locus_tag="VF995_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342000.1"
                     /translation="MLTTQEYAALELLERAGGELHRWPAPRTVAAALRDRHMVALTWR
                     GERVALTLLGRAALAEARRPSRAGVAR"
     gene            2315..>2988
                     /locus_tag="VF995_00095"
     CDS             2315..>2988
                     /locus_tag="VF995_00095"
                     /inference="COORDINATES: protein motif:HMM:NF040183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9342001.1"
                     /translation="MPADHSPTVRRRRLGIELRRLREAADYTIEDVAKHLECSGSKVS
                     RIETGKGMLRIRDVRDMLTLYGLTDDDRRDLLLTMAREGQQKGWWTDYDPPAGLEIFI
                     GLEAEAASYRIYHTHLVIGLLQTEDYARALLRAIHVGDTQEEPERLVELRMRRQSILH
                     REQRPLDLWVVIDEAVLRRPIGGKAVMRAQLLHLIKMCELQNVTLQVLPFARGAHAGL
                     DGPFVV"
BASE COUNT          475 a         1075 c         1024 g          414 t
ORIGIN      
        1 tctggatggc gccggcctcg ggcgccgcga ccagcagccc ccccgccagg ccggccaggg
       61 ccagcccgat cacggcgctc acgactcgtc ccttgtgcat ctcccggacc cccttgaaag
      121 cttcatcacg cgcgacgccg cgctcagtgg tccggtcacg gcccatccca ccactccgcg
      181 atcttggccc caggccctcc cgcgagcatc gggtagacgc tgtaggcgcc ctacagcaag
      241 ttgatgccga gggcaagcac cccggccgcg agcacggcgg ctcccaggta gaccagggag
      301 atcagggcat ttggccgcac ggcccgcggc gaggccaccg acaggaagaa cccgagcggc
      361 atgaggatgg gcggtgcggt caggccccag cggatcagct ccttggcggt gccggagagg
      421 ccggtctgat cgacgtagag catccccacc agcgccagga tcaccagcac gccggcatgg
      481 gcgtgccccg cccggaacag gttctggcgc aggggattgt cgaggtagcc agggatccgc
      541 cgggtgatga accgcagcag cgagatcccc ccgtactcga tggcgaccac ggcgatcagc
      601 agcacgccgg ccgtggacag cgacgcagag gacatgcgaa gtcccttccg aaggccaatc
      661 ttgccactgt caacttaggg ctggcagcga taacttgtca atgtctagat acctggtagc
      721 cttgcagcat gcccgcaggc cctgaagcag tccccgacgc agtccccaac gacgcgggcg
      781 gcgcgccccg gccctatcac cacggcgacc tgcgccgcgc cctcatgcag gccgccctcg
      841 atgcgatcgt ggagggcggg ccagcaaacc tcagcctgcg ggaccttgcc cgccgcgccg
      901 gtgtctcgca tgccgccccg gcccaccact tcggcgacaa ggcaggcctc ctgagcgcca
      961 tcgccgccga ggggttcgag cgcctggccg tcaccctcga ccaggcgtgg cggcagactg
     1021 gcagcttcct ggaggttggc gtcgcctacg tccgcttcgc cgccagccac cgggcccatt
     1081 tcgaggtgat gttccgcccc gagctgtacc gccccgacga cccggcggtc gctcgcgccc
     1141 gcgcggcggc gcgctcgctg ctgtacggcc ccctggccga cagctccggc gcggatccgc
     1201 gcgccgccgt cgccgcgtgg tccctggtgc acggcctcgc caccctgtgg ctcaaccgca
     1261 acctgccgcc gcagctcggc gacgaccccg agcagctcgc ccgcgaggtc gccggctacc
     1321 tgttccggga ccgggaccgg ccgcctcggg cgcggtaggg caccggcggg tcgccggtca
     1381 caggtcgagc gcctgccagg ccggggacgc ggaggtcgtg gcggcggcgg tccgggggcg
     1441 cccgagggca gggagctgtc tcgacctgcc atcggggccg acccggccgg gaccggaccg
     1501 gaggttggcg agcggtcccg gggctggacg cacacgcgcg gcgggcgcgg cgggcgcggc
     1561 ggcgaggccc agcgagccgg cggggtcgcg cgcggcggtc tgggcgaggg agcggacgtg
     1621 ctcctgggcg tggtcgccga tcgcggcgag caggtcggcg tagtcctgat ggaagtcgcg
     1681 ccagcccggc tcctgcccgg tcccggcgtg ctggtgccag gcctggttca cccgggcgtg
     1741 gagggcgtgg atgcgctggt ggacgtcgtc gtgctcgggg gaggggagcg agccgcccac
     1801 aggacgcccc tgtgcgtgcc agtggcgccg tgcctgggac cagtcgaggt tcatcgcgcg
     1861 acccccgccc gtgagggccg gcgggcctca gcgagcgcgg cgcgtccgag gagcgtcagg
     1921 gcgacccgct ccccccgcca cgtcagggcg accatgtgcc ggtcccgcag cgccgccgcc
     1981 accgtccgcg gtgccggcca gcggtggagc tcgccgcccg cccgttcgag cagctccagt
     2041 gcggcgtact cctgcgtggt cagcatccgt ccccccttgg gtcttgcgac cgatccgtga
     2101 cgagcgctac aaccgtgacg ctatgatgca gtgtagagac ccgcgatgca aatgcaagga
     2161 gcagatccac caacaaagtt caccaacaaa tggtaacaac caacaaattg aatatgcgat
     2221 gagtgcgaac agacagaact tcggcgcaga aaccgactcg ccttcctcaa gcgtgcctga
     2281 aacacttgca aagcccttgc agaaggaggc tgccgtgccc gccgatcaca gcccgaccgt
     2341 ccgccgccgg cggctcggca tcgagctgcg acgtctccgg gaggccgccg actacaccat
     2401 cgaggacgtc gccaagcatc tggagtgctc cggctccaag gtgagccgca tcgagaccgg
     2461 caagggcatg ctccgcatcc gcgacgtccg cgacatgctc accctctacg gcctcaccga
     2521 cgacgatcgg cgagacctcc tgctcaccat ggcgcgggag ggtcagcaga aaggatggtg
     2581 gaccgactac gaccccccgg ctggcctcga aatctttatc ggtttggagg cggaagccgc
     2641 gtcctaccgt atttaccaca cccatctcgt cattggactc ctgcagaccg aggactacgc
     2701 ccgggcgctg cttcgtgcga tccacgtggg cgacactcaa gaggagcccg agcggctggt
     2761 cgaactccgg atgcgacggc aatcgatcct ccatcgggaa cagcggccgc tcgacctgtg
     2821 ggtagtgatc gacgaagcgg ttctgcgccg gccgatcggc gggaaggcgg tgatgcgagc
     2881 acagctgttg cacttgatca agatgtgcga actccagaat gtgactctcc aggtgttgcc
     2941 gttcgcacga ggcgcccacg ctggcctcga cggcccgttc gtcgtcat
//
LOCUS       DASWDC010000005         4935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1586378, whole genome shotgun sequence.
ACCESSION   DASWDC010000005 DASWDC010000000
VERSION     DASWDC010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586378"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /gene="phoU"
                     /locus_tag="VF995_00100"
     CDS             <1..586
                     /gene="phoU"
                     /locus_tag="VF995_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX9342002.1"
                     /translation="AAFDLTLADEVVVRDDQLDQSYVEVEEEILRTLALQAPVAKDLR
                     LVAGVLHINAHIERMGDLCVNMARFVRLVKGYPPVPEIMATLTEMGTHAQRMVATAMT
                     SFARRDLDLAERLPILDQPLDQLNERIFRQTEKIDVDHEGAIEWASRMILVARYLERI
                     GDHSVDIGEQVFFIVTGEVRTLVPPKAVRAGLER"
     gene            609..1991
                     /locus_tag="VF995_00105"
     CDS             609..1991
                     /locus_tag="VF995_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9342003.1"
                     /translation="MNLPKPVGPLLAGFLTLALAALIGFVAIAAGTSVPTGALVALGV
                     ALVAVALALAAITARTLGRPLPGLARSVDRLARIALPGWRWTHPNDLTGIAGELGAMV
                     PRLRDRLDELTEERDRASQILDALADGVLLLDGAGRLLVANPSARAWFGLPDDLRPGL
                     PLRRVLGVAQVADLADEAVARRAPVTGIVTVVLPEHRTLSLRAFPLADRGPTGRIVVT
                     LSDITQRRRLEVLRRDFVANASHELKTPVAALRALAESLESAIGDDPPAARRFAARVG
                     REAERLEALVRDLLDLSRVEGRALDVEPLDLVGLVKEVAEPYGERAEERHIRLRTALQ
                     AGTTMRGDRAQLGLLLSNLLDNALRHTGSRGMIRVSVEAADGTVVLQVADTGEGIPTV
                     ELPRVFERFYRVDKARTRRTGGTGLGLAIVRHVAEAHGGLVTVDSEVGKGSTFTVTLP
                     AAGPPPLPTP"
     gene            2142..3113
                     /locus_tag="VF995_00110"
     CDS             2142..3113
                     /locus_tag="VF995_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX9342004.1"
                     /translation="MFDARFLVLLLAIAAGSRGLVAGVLDGPELRTWATIFVSISVQA
                     LPFLVLGVLLSGAVAALVSPAWLARALPGRPLLAVPVASVAGMALPGCECASVPIAAR
                     LVAKGVTPAAALAFMLSAPAVNPVVLVATAVAFPGRPEVVLARFLGSLLTAMVTGLLW
                     SRIGRDDWLMGARRRNQPEAPGALNPDGGDHRWEAFAQTVRGDFLQAGGWLVVGAMAA
                     ASLQVFVPRSLLGQVAGNHLLAALGLGLLAVGLAICSEADAFVAASLRQFSLSARLVF
                     LVVGPAVDVKLIAMQLGTFGRRFVLRWALLTFVVALASGLLVGRWLL"
     gene            3166..3933
                     /locus_tag="VF995_00115"
     CDS             3166..3933
                     /locus_tag="VF995_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEX9342005.1"
                     /translation="MNDQRTQGALLLAVGATALFLSFSDLTLSYVRAGLRPLLGVAGA
                     ILLGLGLLSVLQGRRQAAGGEDEDWHDHDGGHGPRVAWLLVLPVLVLLLVEPPALGSF
                     AALRQSSLTLAGGDGNFPPLARATGGAVPVGLSEFVDRALFDKQHSLAGQRVRVFGFV
                     MPEKQGSYRLARFVMYCCAADAEVVEIVVRGDPTPRQPDQWVTVEGRWLAPGPGPGAA
                     PNTVSDGHGGRLPVLAAETVTVTSPPRDRYEHSIYGV"
     gene            3984..4232
                     /locus_tag="VF995_00120"
     CDS             3984..4232
                     /locus_tag="VF995_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342006.1"
                     /translation="MDEVDIRKLAELVARLSANQQRVVATVAEGFRSGWLDPASWEHA
                     WRDCDLTDDWVLADALTWLARRKRRRARAQITTSGAGA"
     gene            complement(4207..>4935)
                     /locus_tag="VF995_00125"
     CDS             complement(4207..>4935)
                     /locus_tag="VF995_00125"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX9342007.1"
                     /translation="LRTFRSVLDREWSGALRAELAWIADLLGAVRDADVLLERLRHGL
                     TALSEPQADADTGRRLLRGLERRRETDRAVLLEAMDSARYAALRDRVVAAVQAPVLLP
                     AAERSAVELGPGLVARRWRRLRDQVARAGADPSDEDLHRIRIDAKRCRYAAEAVEPVA
                     GEPAKAFAGAVAALQEVLGEQHDAVVAQAWLRQAAGSAGVRRAEAFVAGQLAAAEHAR
                     ALAARAKWRKTWDKAAVKRLRRWL"
BASE COUNT          555 a         1835 c         1852 g          693 t
ORIGIN      
        1 ggccgccttc gacctgacgc tcgccgacga ggtggtggtc cgcgacgacc agctcgacca
       61 gtcctacgtc gaggtcgagg aggagatcct gcggaccctg gcgctgcagg cgccggtggc
      121 caaggacctc cggctggtcg ccggggtgct gcacatcaac gcccacatcg agcgcatggg
      181 cgacctctgc gtgaacatgg cccgcttcgt ccgcctggtc aagggttacc cgccggtccc
      241 cgagatcatg gcgaccctca ccgagatggg cacccacgcc cagcggatgg tcgccaccgc
      301 catgaccagc ttcgcccgcc gcgacctcga cctggcggag cggctgccga tcctcgacca
      361 gccgctcgac cagctcaacg agcgcatctt ccggcagacc gagaagatcg acgtcgacca
      421 cgaaggtgcg atcgagtggg ccagccggat gatcctggtc gcccgctacc tggagcggat
      481 cggcgaccac tcggtcgaca tcggcgagca ggtcttcttc atcgtcaccg gcgaggtccg
      541 cacgctggtc ccgcccaagg cggtccgcgc cgggctggag cgctgacggg cacggctgga
      601 ggcggatcgt gaacctcccg aagccagtcg gcccgctcct ggccggcttc ctgacgctgg
      661 cgctggcggc cctgatcggg ttcgtcgcca tcgccgctgg gacgtctgtc cccacgggcg
      721 cgctggtggc gctcggcgtg gccttggtcg cggtggcgct ggcgttggcg gcgatcaccg
      781 cgcggacgct cgggcggccg ctgccgggcc tggcccggtc ggtcgaccgg ctggcgcgga
      841 tcgcgctgcc gggctggcgc tggacccacc cgaacgacct caccgggatc gccggggagc
      901 tgggcgcgat ggtgccgcgg ctgcgcgacc ggctggacga gctgaccgag gagcgcgacc
      961 gggccagcca gatcctggac gcgctcgccg acggcgtgct gctgctggac ggcgccgggc
     1021 ggctgctggt cgccaacccc tcggcgcggg cctggttcgg gctgcccgac gacctgcggc
     1081 cggggttgcc gctgcggcgc gtcctcgggg tcgcccaggt ggcggacctg gccgacgagg
     1141 cggtcgccag gcgggcgccg gtgaccggca tcgtcaccgt ggtcctgccc gagcaccgca
     1201 ccctgtcgct gcgcgccttc ccgctggccg accggggccc gaccgggcgc atcgtggtga
     1261 cgctctccga catcacccag cgccgccggc tggaggtgct gcggcgcgac ttcgtggcca
     1321 acgcctccca cgagctgaag acgccggtgg ccgcgctgcg cgccctggcg gagtcgctgg
     1381 agagcgcgat cggcgacgac ccgcccgccg cgcggcgctt cgccgcccgc gtcggccgcg
     1441 aggccgagcg gctggaggcg ctggtgcgcg acctgctgga cctctcccgg gtggaggggc
     1501 gcgccctgga cgtggagccg ctcgacctgg tcggcctggt caaggaggtg gccgagccct
     1561 acggcgagcg cgccgaggag cgccacatcc ggctgcggac cgccctccag gcgggcacca
     1621 ccatgcgggg cgaccgcgcc cagctcggcc tgctgctctc caacctgctc gacaacgccc
     1681 tgcgccacac cggctccagg ggcatgatcc gcgtctcggt cgaggccgcc gacggcaccg
     1741 tggtcctgca ggtggccgac accggcgagg gcatccccac ggtcgagctg ccccgcgtct
     1801 tcgagcgctt ctaccgggtc gacaaggccc gcacccgccg caccggcggc accggcctcg
     1861 gcctcgccat cgtccgccac gtcgccgaag cccacggtgg cctggtcacg gtggactccg
     1921 aggtcggcaa gggctccacc ttcaccgtga ccctcccggc agccggcccc ccaccgctcc
     1981 cgactccctg agcgaccctg ggccagggac gcgatagcat ccctgatccc atccgtgttc
     2041 agctcgttca gggcgaggtg atcgccgtca agcaggccag caccagcacg acgactccgg
     2101 ccgctgccgt ggtgacggtg ccgcccgccc gggtccggcg gctgttcgac gcgcggttcc
     2161 tggtgctgtt gctggcgatc gcggcgggaa gccgcgggct ggtcgccggg gtgctggatg
     2221 ggccggagct gcggacctgg gcgacgatct tcgtgtcgat ctctgtccag gcgctgccgt
     2281 tcctggtgct cggggtgctc ctcagcggtg cggtggccgc gctggtctcc cctgcctggc
     2341 tggcgcgggc cctgccggga cggccgttgc tggcggtgcc ggtggcgagc gtggccggga
     2401 tggcgctgcc cgggtgcgaa tgcgcctcgg tgccgatcgc cgcccggctc gtggccaagg
     2461 gggtcacccc cgccgccgcg ctcgccttca tgctctcggc cccggcggtc aacccggtgg
     2521 tgctggtggc caccgcggtc gccttcccgg gacggcccga ggtggtgctg gcccgcttcc
     2581 tcggctcgct cctcaccgcg atggtgactg ggctgctgtg gagccggatc ggccgcgatg
     2641 attggctgat gggcgcccgc cgccggaacc agccggaggc tccgggcgcc ctcaaccccg
     2701 acggcggcga ccaccgctgg gaggcgttcg cccagacggt ccggggcgat ttcctgcagg
     2761 ctggaggctg gctggtggtc ggggcgatgg ccgcggccag cctgcaggtg ttcgtgccac
     2821 gctcgctgct cggccaagtc gccgggaacc acctgctggc cgcgctgggg ctcgggctgc
     2881 tggccgtggg gctggcgatc tgctcggagg ctgacgcgtt cgtggccgcc agcctgcggc
     2941 agttctcgct cagcgcgcgg ctcgtgttcc tggtggtcgg gccggcggtg gacgtgaagc
     3001 tgatcgccat gcagctcggc accttcgggc gccgcttcgt cctgcgctgg gcgctgctga
     3061 ccttcgtggt cgcgctggcg agtggcctgc tggtgggtcg gtggctgctg tgagcggcac
     3121 ggtgagcgcg ctgccgcgac ggacgacgat ggcggagcgc cccgaatgaa tgaccagcgg
     3181 acccagggcg cgctgctgct cgcggtgggg gcgacggcgc tgttcctgag cttcagcgac
     3241 ctgaccctgt cgtacgtccg ggcggggctg cggcccctgc tgggggtggc gggcgcgatc
     3301 ctgctggggc tcggcctcct ctcggtcctc cagggtcgcc ggcaggcggc cggcggcgag
     3361 gacgaggact ggcatgacca tgatggaggg catgggccgc gggtcgcctg gctgctggtg
     3421 ctgcctgtgc tggtgctgct gctggtggag ccgccggcgc tgggctcgtt cgcggccttg
     3481 cggcagtcgt cgctgacgct ggcaggcggg gacggcaact tcccgccgct ggcccgcgcg
     3541 accggcgggg ctgtgccggt agggctcagc gagttcgtcg accgggcgct gttcgacaag
     3601 cagcactccc tcgccgggca acgggtgcgc gtcttcggct ttgtcatgcc ggagaagcag
     3661 ggttcctacc ggttggcccg cttcgtgatg tactgctgcg ccgccgacgc cgaggtggtc
     3721 gagatcgtgg tccgcggcga ccccaccccc cgccagcccg accagtgggt cacggtggag
     3781 ggccgctggc tcgcccccgg acccgggccg ggcgcggcgc cgaacaccgt ctccgacggc
     3841 cacggcggcc ggctccccgt cctggccgcc gagacggtca ccgtgacgag cccgccccgg
     3901 gaccgctacg agcacagcat ctacggggtc tgagcggcag ccgcttcgtc aagtcgccac
     3961 ccgcaccgtc cgagagagcc ttcgtggacg aggtcgacat caggaaactc gccgagctgg
     4021 tcgcccgcct ctcggccaac cagcagcggg tggtcgccac cgtcgccgag ggcttccggt
     4081 ccggctggct cgacccggcg agctgggagc acgcctggcg cgactgcgac ctgaccgacg
     4141 actgggtgct cgccgacgcc ctcacctggc tggcccgccg caagcgccgc cgcgccaggg
     4201 cccagatcac aaccagcggc gcaggcgctt gacggcggcc ttgtcccagg tcttgcgcca
     4261 tttggcgcgc gcggcgaggg cacgggcgtg ctcggcggcg gcgagctggc cggcgacgaa
     4321 cgcctcggcg cggcggaccc cggccgaccc ggccgcctgg cgcagccagg cctgggcgac
     4381 caccgcgtcg tgctgctcgc cgaggacctc ttggagggcg gcgacggcgc cggcgaaggc
     4441 cttggcaggc tcgccggcca ccggctcgac cgcctcggcg gcgtagcggc agcgcttcgc
     4501 atcgatccgg atccggtgca gatcctcgtc gctggggtcg gcgcccgccc gcgccacctg
     4561 gtcgcggagc cgccgccagc gccgggcgac cagccccggg cccagctcga cggcgctccg
     4621 ctccgcggcg ggcagcagta ccggcgcctg gacggcagcg accacgcggt cgcgcagggc
     4681 cgcataccgg gccgaatcca tcgcctccag caggacggcc cggtctgtct cgcggcgccg
     4741 ctccagcccg cggagcagcc gccgccccgt gtccgcgtcg gcctgcggct ccgacagggc
     4801 ggtcaggccg tgccgcaggc gctccagcag cacgtcggcg tcgcggaccg cgcccagcag
     4861 atcggcgatc caggccagct cggcgcgcag ggcgccgctc cactcccggt cgagcaccga
     4921 ccggaaggtc cgcag
//
LOCUS       DASWDC010000006         5795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_668112, whole genome shotgun sequence.
ACCESSION   DASWDC010000006 DASWDC010000000
VERSION     DASWDC010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668112"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VF995_00130"
     CDS             <1..788
                     /locus_tag="VF995_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007535530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9342008.1"
                     /translation="LGPLSPLEVSDREDELGDWLEGHGVDGGWALAPPLVAAGVDTAW
                     LERVAATVPAGLLPPAVRLAAAACETAGLLDELAEAAGRITRLIGQAKQYTQMDRAPL
                     QQVDVHDGLDSTLAMLAHKLQAGTGIEVVRDYDRSLPKLLAYAGELNQVWTNLIDNAV
                     DAMDGHGTLTVRTRRDHGGDRNGDCVLVEIGDTGPGVPEHVQAHIFEPFFTTKPVGKG
                     TGLGLDICWRIVVQRHGGDLRVVSAPGDSRFRVLLPVGGPPGS"
     gene            complement(794..1870)
                     /locus_tag="VF995_00135"
     CDS             complement(794..1870)
                     /locus_tag="VF995_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9342009.1"
                     /translation="MVVPYTAVGLVTTVRGVRRRGEIKRNLEHVADVMGAASWLSSLD
                     LPVRLVAIPEGALQGFTDEVFDLDHATYAEEVAIDVPGEETAILSELARHWNVFIMAQ
                     AKARNEHFPGRFFNVGFVIDPTGEVILRHHKVATLYPVEHSVVPHDVWDRWIELYGRD
                     LDAFYPVADTEIGRLGIMMANEGSYPENARALAMNGAEVVYRASYPHPHTGNEFFEIQ
                     NRARALDNNFYVVAPNLGTYYLEQDSAVPVDTFGGRSMVVDYKGRVVGQHAYGAGASY
                     VAGTIDIQALRDFRARAQWDNWLKDLRTEQYQLIYEQPIYPKNLYLDRAPYTHAEFRR
                     EVIDKQVEKLHQLGIWAKPAPAGE"
     gene            2025..2660
                     /locus_tag="VF995_00140"
     CDS             2025..2660
                     /locus_tag="VF995_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9342010.1"
                     /translation="MSIRHALLALLSEGPKYGLQLRQEFEERTGEVWPLNVGQVYQTL
                     QRLERDELVASDDTGVEGPQKAFQITPDGEREIAAWLRTPPDLTSPPRDELVIKVLVA
                     LRVPGVDVREVIQAHRRYLVELMQQWTRLKEDEARFDLGLALVVDAELFRLDSVVRWL
                     DAADGRLKRATVDPPDSATSPTPRLRLRIGAPTRPRVSAAGERTTTPERQS"
     gene            2657..3253
                     /locus_tag="VF995_00145"
     CDS             2657..3253
                     /locus_tag="VF995_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342011.1"
                     /translation="MSALELHRVAKVYGEGPTEVHALRGVDLSVDPGAMVAVMGPSGS
                     GKSTLLTIAGSLEDPTSGEVLVGGTALAGMSRNQRARLRRRSIGYVFQDFNLLPGLTA
                     AENVTLPLELDGVRARKARAAGMQALEELGLADRAGHFPDQLSGGERQRVAIARAVVG
                     DRRLLLADEPSGALDSENGEVVMRLLLAACCSPPAAAA"
     gene            3259..3393
                     /locus_tag="VF995_00150"
     CDS             3259..3393
                     /locus_tag="VF995_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342012.1"
                     /translation="MVVTHDAQLASWADRVVFLRDGRVADQTVPPPGPESLLTSGPNS
                     "
     gene            3390..>5795
                     /locus_tag="VF995_00155"
     CDS             3390..>5795
                     /locus_tag="VF995_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9342013.1"
                     /translation="MSTALRQRPAEARDNGRGGGAAARRAVIRWAWRLFRREWRQQLL
                     VLALLSVAVAATVLGAAVGSNAPSSPMATFGNANHLVVLPGSDRTLAADLAAIRQRFG
                     TIGVIEHQRLSTGSVNPVDLRAQDPKGPFGSPMLRLDAGRYPARPGEVAVTSQVASLY
                     NLHVGDLWNAGGHALRVVGLVENPGNLLDSFALVAPGQISAPTQVTVLFNASEARITS
                     FAFPGGVSAETPPPPPAGFSPAIVVLALSTFGLIFIGLVAVAGFTVMAQRRLRALGML
                     SSLGATDRDVRLVLLANGAVVGVVATLAGAAAGFLAWVAYAPRLQTSTGHRIDRLNLP
                     WWAIGTAMGLAVVTAVAAARRPARSAARMPVVAALSGRPATPKAAHRSAVPGAVLLVA
                     GLGLLWFSGGWGGSSNADQLKTLGGIAATALGSLLLAPLCVAALGAIGGHAPVGARVA
                     LRDLARYRARSGAALAAVTFAVLTAVLICVLATARFANPVDYFGPNLPANQLVVYAPG
                     GTPGVNGGPGSAQTPAQLRSLRAHVDAIAAALGTHDVLELDSPIKPATTGTAQFGQLT
                     DGQIQSGGQLYVATPALLAHYGINPNQIDASADLLTSRAGLASTARLYLYGDSGPEIS
                     VSPGGPPPSGSGSPGGPGPGGPTSFSCAPGSCVANPAIQTLSRLPTDTSDPNLLITAH
                     GMDRLKSFGLQAAPSAWIIQTARPLTAVQINAARQAAVAGGATVETKSQNPSLAELRD
                     WAAAAGILLALGVLAMTVGLIRSETAGDLRTLTATGANSRTRRTLTSATAGALGLLGG
                     LLGT"
BASE COUNT          763 a         2163 c         2049 g          820 t
ORIGIN      
        1 agctgggccc actctcgccg ctggaggtat ccgatcgtga ggacgagctg ggcgactggc
       61 tggaggggca cggcgtcgac ggtggctggg cgctcgcgcc gccgctggtg gccgccgggg
      121 tcgacaccgc ctggctggag cgggtggccg ccaccgtgcc cgccgggctg ctcccgcccg
      181 cggtgcggct ggccgcggct gcctgcgaga ccgccggcct gctcgacgag ctggccgaag
      241 cggccgggcg gatcacccgg ctgatcggtc aggccaagca gtacacccag atggaccggg
      301 cgccgctgca gcaggtcgac gtccacgacg ggctcgactc gaccctggcg atgctggcgc
      361 acaagctcca ggccggcacg ggcatcgagg tggtgcggga ctacgaccgc tcgctgccca
      421 agctgctcgc ctacgcgggc gagctgaacc aggtctggac caacctgatc gacaacgccg
      481 tcgacgccat ggacggccac gggacgctga cggtccgcac ccgccgcgat cacggcggtg
      541 accggaacgg cgactgcgtc ctggttgaga tcggcgatac cgggccgggc gtccccgagc
      601 acgtccaggc ccacatcttc gagccgttct tcaccaccaa gccggtcggc aagggcaccg
      661 gcctcggcct cgacatctgc tggcggatcg tggtgcaacg ccacggcggc gatctccgcg
      721 tcgtctccgc cccaggtgac agccgcttcc gggtgctgct gccggttggc ggcccaccag
      781 gatcgtagcc gcctcactcg cccgctggcg ctggcttggc ccagatgccc agctggtgga
      841 gcttctcgac ctgcttgtcg atcacctcgc ggcggaactc ggcgtgggtg tacggcgcgc
      901 ggtccaggta gaggttcttg gggtagatcg gctgctcgta gatgagctgg tattgctcgg
      961 tgcgcaggtc cttgagccag ttgtcccact gtgcgcgggc gcggaagtcc cgcagcgcct
     1021 ggatgtcgat cgtgccggcg acataggagg cgcccgcgcc gtaggcgtgc tggccgacca
     1081 cgcgaccctt gtagtcgacc accatcgagc ggccgccgaa ggtgtcgacc ggcacggcgg
     1141 agtcctgctc caggtagtag gtgccgaggt tcggcgcgac cacatagaag ttgttgtcca
     1201 gcgcccgcgc gcggttctgg atctcgaaga actcgttgcc ggtgtggggg tgcgggtagg
     1261 aggcgcggta gacgacctcg gcgccgttca tcgccagggc gcgggcgttc tccgggtagg
     1321 acccctcatt ggccatcatg atcccgagcc gcccgatctc ggtgtcggcc accgggtaga
     1381 acgcgtccag gtcgcgcccg tacagctcga tccagcggtc ccagacgtcg tgggggacca
     1441 ccgagtgctc gaccgggtag agggtcgcca ccttgtggtg gcgcaggatg acctcgccgg
     1501 tcgggtcgat cacgaagccg acgttgaaga agcggccggg gaagtgctcg ttgcgggctt
     1561 tggcctgggc catgatgaag acgttccagt ggcgcgccag ctcgctgagg atggcggtct
     1621 cctcgccggg cacgtcgatg gcgacctcct cggcgtaggt ggcgtggtcc aggtcgaaga
     1681 cctcgtcggt gaatccctgg agggccccct cggggatcgc caccagccgc accggcaggt
     1741 ccaggctgga gagccacgac gccgccccca tcacgtcggc gacgtgctcc aggttgcgct
     1801 tgatctcgcc cctgcggcgc accccccgga ccgtggtcac cagcccgacc gctgtatacg
     1861 gaacgaccac cccagccacc tcctcgccgg cgcctaccgg gcgaccctac tgcgcgcccg
     1921 aagcgccagc aatgccggaa tccccgccgg ggcgcgagtc cgggcttgcg gtcgcttgac
     1981 tctgccagta tgatactgag tatgctactg gaaagggtga gacgatgagc atccgccatg
     2041 ccctgctcgc gctgctgagc gagggcccca agtacggtct gcagctccgg caggagttcg
     2101 aggagcggac cggcgaggtc tggccgctga acgtgggcca ggtgtaccag acgctgcaac
     2161 ggctggagcg tgacgagctc gtcgcctccg acgacaccgg ggtggagggg ccgcagaagg
     2221 ccttccagat cacccccgac ggtgagcggg agatcgccgc gtggctgcgc accccgccgg
     2281 acctgacctc cccgccccgc gacgagctgg tcatcaaggt gctggtggcc ctccgggtgc
     2341 ccggcgtcga cgtccgcgag gtcatccagg cccaccgccg ctacctggtg gagctgatgc
     2401 agcaatggac ccgcctcaag gaggacgaag cccgcttcga cctggggctg gcgctggtgg
     2461 tcgacgccga gctgttccgg cttgactcgg ttgtccgctg gctggacgcc gccgacggcc
     2521 gcctgaagcg cgccaccgtc gatcctcccg actccgcgac ctcgccgacg ccgaggctgc
     2581 ggctgcgcat tggagcgccg acgcgcccca gggtgagcgc ggcaggcgag cggacgacga
     2641 cgccggagcg ccagtcatga gcgccctgga gctgcaccgc gtcgccaaag tctacggcga
     2701 gggtccgacc gaggtgcacg cgctccgcgg ggtggacctg tcggtcgacc ctggggcgat
     2761 ggtggcggtg atgggaccga gcgggtcggg caagagcacg ctgctgacca tcgctggcag
     2821 cctcgaggac ccgaccagcg gcgaggtgct ggtcggcggc accgcactgg cgggcatgtc
     2881 gcgcaaccag cgggcccggc tgcggcgccg ctcgatcggc tacgtgttcc aggacttcaa
     2941 cctgctgccc gggctgaccg cggccgagaa cgtcacgctg ccgcttgagc ttgacggcgt
     3001 ccgcgccagg aaggcccgcg ccgccggcat gcaggccctg gaggaactcg gcctcgccga
     3061 ccgggcaggg cacttccccg accagctctc cggcggcgag cgccagcggg tggcgatcgc
     3121 cagggccgtg gtcggcgacc ggcgcctgct gctggccgat gagccctcgg gcgccctcga
     3181 ctcagagaac ggggaggtgg tgatgcgcct gctgctcgcc gcctgctgct cgccgcctgc
     3241 cgccgcggcg tagccgccgt ggtggtgacc cacgacgcgc agctggcctc gtgggccgac
     3301 cgggtggtgt tcctgcgcga tgggcgtgtc gccgaccaga ccgtgccgcc cccaggtccg
     3361 gagtcgctcc tcacctctgg cccgaactca tgagcacggc actgcggcaa cggccggccg
     3421 aggcgcgcga caacggccgc ggtggcggcg cggccgcccg ccgcgccgtc atccgctggg
     3481 cgtggcggct gttccgccgc gagtggcgcc agcagctgct ggtgctggcg ctgctcagcg
     3541 tcgcggtggc ggcgacggtc ctcggcgcgg cggttggcag caacgcgcca tcgtctccca
     3601 tggccacctt cgggaacgcc aaccacctcg tggtcctccc cgggtccgac cggacgctcg
     3661 ccgccgacct cgcggccatc cggcaacgtt tcggcaccat cggggtgatc gagcaccagc
     3721 ggctcagcac cggctcggtc aaccccgtgg acctgcgggc gcaggacccg aagggcccct
     3781 tcgggtcccc gatgctgcgg ctggacgccg ggcgctaccc ggcccggccg ggcgaggtcg
     3841 cggtgaccag ccaggtggcg tccctttaca acctgcacgt gggcgacctc tggaacgcgg
     3901 gcggccatgc gctgcgggtc gtcggcctgg tcgagaaccc cggcaacctg ctggacagct
     3961 tcgcgctggt ggcgccgggg cagatcagcg cgccgactca ggtgacggtg ctgttcaacg
     4021 ccagcgaggc gcgaatcacc tcgttcgcct tccccggcgg cgtcagcgcc gagacgcctc
     4081 cgccgccacc ggccgggttc agcccggcga tcgtggtgct cgccctctcc accttcgggc
     4141 tgatcttcat cggcctggtc gccgtggccg gcttcacggt gatggcccag cgccggctgc
     4201 gggcgctcgg catgctcagc tcgctcggcg cgaccgaccg cgacgtgcgg ctggtactgc
     4261 tcgccaacgg cgccgtcgtc ggggtggtcg ccacgctcgc cggcgccgcg gcggggttcc
     4321 tcgcctgggt cgcgtacgcg ccgcgcctgc agaccagcac cgggcaccgc atcgaccgcc
     4381 tcaacctgcc ctggtgggcg atcgggacgg cgatgggcct cgcggtggtg actgccgtcg
     4441 ccgccgcccg gcggccggcg cgctcggccg cccggatgcc ggtggtcgcg gccctgtcgg
     4501 gacgcccggc gacgccgaag gcggcgcacc gctccgccgt gcccggcgcc gtcctgctgg
     4561 tggccggcct cggcctgctc tggttcagcg gcgggtgggg cggcagcagc aacgccgacc
     4621 agctgaagac gctcggcggc atcgccgcga ccgcgctcgg gagcctgctg ctcgcgccgc
     4681 tctgcgtcgc ggcgctgggc gcgatcggcg ggcacgcgcc ggtcggggcg cgggtcgcgc
     4741 tgcgcgacct cgcccgctac cgcgcccgtt ccggggcggc cctcgcggcg gtcaccttcg
     4801 cggtcctgac cgccgtgctc atctgcgtcc tcgccaccgc ccggttcgcc aaccccgtcg
     4861 actacttcgg gcccaacctc cccgcgaacc agctggtcgt ctacgccccg ggcggcaccc
     4921 cgggggtcaa cggcggccct ggctccgcgc agaccccggc ccaactgcga agcctccgcg
     4981 ctcatgtgga cgcgatcgct gccgcgctcg ggacgcacga cgtcctcgaa ctcgactcgc
     5041 ccatcaaacc ggcaaccacc gggacggcgc agttcggaca gctcaccgac ggccagatcc
     5101 agagcggcgg tcagctgtac gtcgccaccc ccgcgctcct cgcccactac gggatcaatc
     5161 cgaaccagat cgacgccagc gccgacctgc tcacctcgcg tgcgggactt gccagcacag
     5221 cccgcctgta cctctatggc gactccggcc ccgagatctc cgtcagccct ggcggtccgc
     5281 cccctagtgg ctccggcagt cctggcggtc ccggccccgg tggtcccacc agcttcagct
     5341 gcgctcccgg cagctgcgtc gcgaacccgg cgatccagac cctcagccgg ctcccgaccg
     5401 acacctccga ccccaatctg ctgatcacgg cccacgggat ggacaggctc aagagcttcg
     5461 ggctgcaggc ggccccctcc gcctggatca tccagaccgc ccggccgctc accgccgtcc
     5521 agatcaacgc cgcccggcag gccgcggtcg ccggcggcgc gaccgtcgag acaaaatcgc
     5581 agaacccgtc gctcgccgag ctgcgcgact gggccgccgc ggccggcatc ctgctcgcgc
     5641 tcggcgtgct cgccatgacc gtcggcctga tccgcagcga gaccgccggt gacctccgca
     5701 cgctcaccgc caccggggcc aacagcagga cgcgccgcac gctcaccagc gccaccgccg
     5761 gcgccctcgg cctgctcggc ggcctgctcg gcacc
//
LOCUS       DASWDC010000007         6052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_584712, whole genome shotgun sequence.
ACCESSION   DASWDC010000007 DASWDC010000000
VERSION     DASWDC010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584712"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..896)
                     /locus_tag="VF995_00160"
     CDS             complement(261..896)
                     /locus_tag="VF995_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342014.1"
                     /translation="MELLILQNMSMQAGRLGRPREALQLVTTVLNAGGLSPRVEALFT
                     IREARTRAQLGERVTAPRGAFAKARSLFQDGIAGTDPAWVWWVTDRELAWHEAMCLAD
                     LGDWAQAVELFELAARRCPVDLNRTRYNDFAHLVQAFVEVGAWRETEPVITQILPYVG
                     EVGSRRTALLLISAAGRIANASVAPPSTRDAAVHLLELLKAVGYETARRYA"
     gene            complement(869..1513)
                     /locus_tag="VF995_00165"
     CDS             complement(869..1513)
                     /locus_tag="VF995_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342015.1"
                     /translation="MGERLRAHRKRLDLTLDAMGDALSSVVWELHQRRVAISGDRVSK
                     WERGVCRPTKLYREAYRVFLDATDEELGFRVVTPAARRPDAAAPAAAAPQLDLFLLEP
                     FVATQPVDAGYIASLRSATAALVTLDNLFGGDDLSALAVRAFRTAHRRLGSGQREEAI
                     ERDLLAATGELAEVAAWMLYDADNQRPPVSSIRRRCCCRGSRATARWNSSSSRT"
     gene            complement(1683..3149)
                     /locus_tag="VF995_00170"
     CDS             complement(1683..3149)
                     /locus_tag="VF995_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342016.1"
                     /translation="MPVSRRTVLKAGGTIGASVAALTVLEQLAWEPIRGVAEAATLPD
                     IQFDIGNFIAPALNVDDGGGAINVRFGPVYTVFVTAALRVAPSRNAQSVLSQGLNLIE
                     AAYPFSPSGVFTFVAYSVQYFNLLPASLVASAIPRLSDGSGRFALERATPSPTDVVSG
                     NGVTKRKFNVPLRLESNHVLLTLRSDSTTVLNDILNWLRGSNVLHGSVVPSILTQLFS
                     FTSSRLMFQQRGLPKKVAQQNGLSFANQVRDTSPMWMSFADQQTSGSAASGNIVTFAG
                     SSHAVLTTARPGSYFDNAAVQHLSHNIQDLASWYDTTGAVSGEAGGETYLERVQYMFR
                     SNPVPNRGVTDQFTNAGGPAFLPNAFNGTGDAAANAQAVGTEAGEHRLGHLSALQRSS
                     RAGDGTPLHIRMDGPGFDNMDVPDGSRQPKLQFTVFVPTAKFFADMRTNQASLDLQAA
                     NNVDPQDNGLERFITATRRQNFLVPPRRHRAFPLAEFL"
     gene            complement(3350..4195)
                     /locus_tag="VF995_00175"
     CDS             complement(3350..4195)
                     /locus_tag="VF995_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX9342017.1"
                     /translation="MRRSLTERELAILRLISDGHTSGEISQALGVSRRTVEYQKRQIF
                     AKLGVRSQAHAVALAARAGLLRPGTPVAESPGQSQQSREEGRAPRRHPVVLGRPSPVL
                     ARLACILVRCELPEPPEPPVAVLVDPEPGDWQAAAELGARIVLVPERDLDPAMAVEAL
                     LRGADAVVSATQESRKLVATVELVGAGQVVLDPLQARMLVDAARGQLEAHGRGGEAPA
                     PTRREREIFASIDRGESVKQTARALGISVKTVENLQRRLFRKLGVRNRAQAVAAAHAR
                     GLLCR"
     gene            complement(4256..5884)
                     /locus_tag="VF995_00180"
     CDS             complement(4256..5884)
                     /locus_tag="VF995_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342018.1"
                     /translation="MSPLRRPTLRRGLLAALAVLPLVLATAAAFGGRTSTPAGSPGGG
                     TPGTSSHFQLVGHDPLFGRGMNAALALYGRYVYVGNRTDGSDTCGVGDPRAGVSPCPH
                     PHPGILIVDTLDPAHPKDVGEIGQPYAALTGITTRELRVWPQAKLLMVMSFRCSSVIH
                     ACPAGTDAQFPFDFKFFDLSDPVHPRFISSYVPTSKAGVAIKPHEMYLWVDPHNARRA
                     LLWVSFPSTSVDPARPNFEILDLSKVPAGSAPVEIAEANWDQFFPGADNPANYDFDLA
                     LHSMAPTADGKVTYLAHLRGGTGVLDTSAVVADTDDHFIDLTNSLLSPAPGSFVHWGA
                     GNVCAGGTAAGCSESHSAVPVPGRPYELNTDEVYGTFTTPSFGWPWGWMRLLDVHDPA
                     TPKLVGQYQIFQNTPAFQPSVDPATEQFTSYSSHNPTVTPDLAVIAWHSGGLQAIDIT
                     NPAKPTQAGWYSPTPLASVATEDPALNRGSNKVTMWSYPIVHNGLIYVIDIRNGLYIL
                     RYTGPHAGDLASLRFLEGNSNLGDAVRLSQVHGG"
BASE COUNT          917 a         1954 c         2264 g          917 t
ORIGIN      
        1 gcgttcacgc ccttgccgcc gcacaggccg tggagggtag cgtcgaccgc cacataccgg
       61 cctcggacga ccgcctcgcg aagcagctcc agcagcgcgg cgaccagccc ccgatcgagg
      121 gtgtcctcgg cacatcccaa cgcttgttcc atctgatcac gttcccatct gcttcggcgt
      181 atctggctcg atagcctcaa tcggaaggga cggtagaacg cggattgacg accgggaagg
      241 tcgagagacc agtcggattt ctaggcgtat cgccgcgctg tctcgtagcc cacggccttg
      301 agcagttcca gcaggtggac ggctgcgtca cgggtcgatg gcggcgctac cgaggcattg
      361 gcgatccgac ctgcggcgct gatgagcagc agcgcggttc gccgcgagcc gacttcgccc
      421 acgtagggca ggatctgcgt gattaccggt tcggtctcac gccacgctcc cacttcaacg
      481 aacgcctgga ccaggtgcgc gaagtcgttg taccgcgtgc ggttgagatc gacaggacaa
      541 cgccgcgccg ccagctcaaa cagctcgacc gcttgggccc agtcgccaag gtcggcgagg
      601 cacatggcct catgccaggc gagttcacga tcggtgaccc accacaccca tgccgggtcg
      661 gtgccggcga tgccgtcttg gaacagcgac cgcgccttcg cgaaggcacc gcgcggcgcg
      721 gtaacgcgct cgccaagctg tgcgcgggtc cgagcctcgc ggatcgtgaa gagcgcctcg
      781 acgcgaggcg acagcccgcc agcattgagc acggtggtca ccagctggag cgcctctcgc
      841 ggtcgcccga ggcgcccggc ctgcatgctc atgttctgga ggatgaggag ttccatcgcg
      901 cggtcgcccg agagccgcga caacagcagc gcctcctgat tgagctgacg ggcggccgct
      961 ggttgtcggc gtcatatagc atccaggccg cgacctcggc gagctcccca gtcgccgcca
     1021 gcaggtcccg ctcgatcgct tcctcacgct ggccggagcc gaggcgccgg tgggcggtgc
     1081 ggaacgcacg aaccgcaagc gcggacagat cgtcgccgcc gaagagattg tcgagcgtta
     1141 ccagcgcggc tgtggcgctg cgcaacgagg cgatatagcc cgcgtcgaca ggttgcgtgg
     1201 cgacgaacgg ttcgagcagg aacaggtcga gctgtggcgc tgcagccgcg ggcgcggcgg
     1261 cgtccgggcg gcgagcggcc ggagtcacga cgcggaatcc aagctcctca tcggtggcat
     1321 cgagaaagac gcggtaggcc tcgcggtaga gcttcgtggg acggcacact cctcgctccc
     1381 acttcgacac ccggtcgccg ctgatggcga ctcgccgttg gtgcaactcc cacacgacgc
     1441 tcgacagagc gtcgcccatg gcgtccaagg tcaggtcgag ccgctttcga tgcgcgcgga
     1501 ggcgctcgcc catcgtgagg agcttggaag gctctgccat gccgggcctc caccaggctt
     1561 tgacggctcc cgtgccgctc cgggcggcac gggagcattg tctggtacag gcgaccctac
     1621 cggagccgca cgagcgcgcg ccagtcagcg cccgcgggtg cgacgggcgg tgccgagtcc
     1681 gctcagagga actcggcgag cgggaaggcg cggtggcggc ggggtgggac caggaagttc
     1741 tggcggcggg tggcggtgat gaagcgttcc aggccgttgt cttgcgggtc gacgttgttg
     1801 gccgcctgca ggtcgaggga ggcctggttg gtgcgcatgt ccgcgaagaa cttggcggtg
     1861 gggacgaaca cggtgaactg gagcttgggc tggcggctgc cgtccggcac gtccatgttg
     1921 tcgaaccccg ggccgtccat gcggatgtgc aggggcgtcc cgtcgcccgc gcgcgaggag
     1981 cgctgcaggg cgctgagatg gccgagccgg tgctcgccgg cctcggtgcc gacagcctgg
     2041 gcgttggcgg cggcgtcacc ggtgccgttg aaggcgttcg gcaggaaggc cgggccgccg
     2101 gcgttggtga actggtcggt gacgccccgg ttgggcaccg ggttggagcg gaacatgtac
     2161 tgcacccgct ccaggtaggt ctccccgccg gcctcgccgg agacggcgcc ggtggtgtcg
     2221 taccacgacg ccaggtcctg gatgttgtgg gacagatgct ggacggcggc gttgtcgaaa
     2281 tagctgcccg gccgggcggt ggtgagcacc gcgtggctgc tgcccgcgaa ggtgacgatg
     2341 ttgcccgacg cggcgctccc gctggtctgc tggtcggcga agctcatcca catcggcgag
     2401 gtgtcgcgca cctggttggc gaacgacagc ccgttctgct gcgcgacctt cttgggcagg
     2461 ccgcgctgct ggaacatcag ccggctggag gtgaagctga acaactgcgt caggatcgac
     2521 ggcacgacgc tgccatggag cacgttggag ccgcgcagcc agttgaggat gtcgttgagg
     2581 accgtggtcg agtcgctgcg cagggtgagc agcacgtggt tgctctccag ccgcagcggc
     2641 acgttgaact tccgcttggt gacgccgttg ccggacacca cgtcggttgg cgacggcgtc
     2701 gcccgctcca gggcgaagcg gccgctgccg tcgctgagcc gcgggatcgc cgaggccacc
     2761 agcgaggccg gcaacaggtt gaagtactgc acgctgtagg cgacgaaggt gaagacgccg
     2821 ctcgggctga acgggtaggc ggcctcgatc aggttcagcc cctgcgaaag caccgactgg
     2881 gcgttccggc tcggggcgac gcgcagggcg gcggtgacga acaccgtgta gacggggccg
     2941 aagcgtacgt tgatcgcacc gccgccgtcg tccacgttga gtgccggggc gatgaagttg
     3001 ccgatgtcga actggatgtc gggcagcgtg gccgcctcgg cgacgccacg gatcggctcc
     3061 cacgcgagct gctccaggac ggtgagggcg gcgacggagg caccgatggt ccctccggct
     3121 ttcagaacgg ttcggcggct gacaggcatg acgacctccg gggggcgcat gaaacctgga
     3181 taacctgggt agagtgtaag aagcacccgt ccacgccgaa tagtgataaa ctcccccaca
     3241 tagttgtcgg gggtatttct cacataggga tgaaaatccc ccaggctctg ctttggggga
     3301 aatgcggccc cgctggtgcc gcggctcagc ggctcagcgg ctcagcggct cagcggcaga
     3361 gcaggccgcg ggcgtgggcg gcggcgaccg cctgggcgcg gttgcgcacg ccgagcttgc
     3421 ggaacaggcg gcgctgcagg ttctcgaccg tcttgacgga gatgcccagg gcgcgggcgg
     3481 tctgcttcac cgactcgccg cgatcgatcg aggcgaagat ctcccgctcg cgccgggtcg
     3541 gtgccggcgc ctcgccgcca cggccatgcg cctccagctg gccgcgggcg gcgtccacca
     3601 gcatccgcgc ctgcagcggg tcgagcacga cctggccggc gccgaccagc tcgacggtgg
     3661 cgaccagctt ccgggattcc tgggtcgctg acacgaccgc gtcggcgccg cgcagcagcg
     3721 cctcgaccgc catcgccggg tccaggtcgc gttcggggac cagcacgatg cgagcgccca
     3781 gctcggcggc ggcctgccag tcgccaggct cggggtcgac cagcaccgcg accggaggct
     3841 cgggcggctc gggcagctcg caccgcacca ggatgcaggc gagccgggcc agcaccgggc
     3901 tcggccggcc gagcaccacg gggtggcgcc ggggcgcccg gccctcctcc cgagactgct
     3961 ggctctggcc cggggactcc gccaccggcg tccccggccg cagcaggccc gcccgcgcgg
     4021 ccagggcgac cgcgtgggcc tgcgaccgga cgcccagctt ggcgaagatc tgccgcttct
     4081 ggtactcgac cgtccggcgg ctgacgccga gtgcctgcga gatctccccg ctggtgtggc
     4141 cgtcgctgat cagccgcagg atcgccagct cgcgttcggt gagggatcgc cgcatctccc
     4201 accctttcag acctggaggg cggctccggg cgccgccctc cagcgccaga tgcggctagc
     4261 cgccgtgaac ctggctgagc cggacggcgt cacccaggtt cgagttcccc tccaggaagc
     4321 gcaggctggc gaggtcgccg gcgtggggtc cggtgtagcg caggatgtac aggccgttgc
     4381 ggatgtcgat cacgtagatc agcccgttgt ggacgatcgg gtagctccac atcgtcacct
     4441 tgttggaccc gcggttgagc gccgggtcct cggtggccac cgacgccagc ggcgtggggg
     4501 agtaccagcc ggcctgggtg ggtttggccg ggttggtgat gtcgatcgcc tgcaggccgc
     4561 ccgagtgcca ggcgatcacc gccaggtcgg gggtgacggt ggggttatgc gaggagtagg
     4621 aggtgaactg ctcggtcgcc gggtccacgc tcggctggaa cgccggggtg ttctggaaga
     4681 tctggtactg gccgaccagc ttgggtgtgg cggggtcgtg gacgtcgagc agccgcatcc
     4741 agccccacgg ccagccaaac gacggggtgg tgaaagtgcc gtagacctcg tcggtgttca
     4801 gctcgtaggg ccgcccgggc actggcaccg ccgagtggct ctccgagcag ccggcggcgg
     4861 tccctccggc acagacgttg ccggcgcccc aatgcacgaa gctgccgggg gcggggctga
     4921 gcaggctgtt ggtgaggtcg atgaagtggt cgtcggtgtc ggcgaccacc gcgctggtgt
     4981 ccagcacgcc ggtcccgccg cgcaggtgcg ccaggtaggt gaccttgccg tcggcggtcg
     5041 gtgccatcga gtgcagcgcc aggtcgaagt cgtagttggc ggggttgtcg gcgccgggga
     5101 agaactggtc ccagttggcc tcggcgatct cgaccggcgc gctgcccgct gggaccttgg
     5161 agaggtcgag gatctcgaag ttcggccgcg ccgggtcgac cgaggtcgag gggaacgaca
     5221 cccacagcag cgcacgcctg gcgttgtggg ggtcgaccca caggtacatc tcgtggggct
     5281 tgatggccac gccggccttg gaggtcggca cgtagctgga gatgaaccgg gggtggacag
     5341 ggtcggagag gtcgaagaac ttgaagtcga acgggaactg ggcgtcggtg cccgccgggc
     5401 aggcatggat caccgagctg cagcggaagc tcatcaccat cagcagcttc gcctgcggcc
     5461 agacgcgcag ctcccgggtg gtgatgccgg tcagcgccgc gtagggctgc ccgatctcgc
     5521 cgacgtcctt cggatgggcc gggtcgagcg tgtcgacgat caggatcccg ggatgcgggt
     5581 gcgggcaggg cgagaccccg gcacgcgggt cgcccacccc gcaggtgtcg gacccgtcgg
     5641 tgcggttgcc gacatagacg tagcggccgt agagcgccag cgcggcgttc atcccacggc
     5701 cgaacagcgg gtcgtggccg accagctgga agtgggacga ggtcccgggc gtgccgccgc
     5761 ccgggctgcc ggcaggggtg gatgtcctgc cgccgaaggc agccgcggtg gcgagcacca
     5821 ggggcaggac cgcgagggcc gcgagcaggc cgcgtcgcag tgtggggcgt cgcagggggg
     5881 acatggggat caccctcctc ctgccggcaa cgttgccggc agctcagcac cgatcatggg
     5941 gtggatgtcg gtgcggccgg gggtttgtgc agcgtgaggg gtcacatcat cgccgatggg
     6001 agaattgtga cgataaacgc gctgtacatc aacgaatgtg atgaaattcg ca
//
LOCUS       DASWDC010000008         4756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_668499, whole genome shotgun sequence.
ACCESSION   DASWDC010000008 DASWDC010000000
VERSION     DASWDC010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668499"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VF995_00185"
     CDS             complement(<1..345)
                     /locus_tag="VF995_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9342019.1"
                     /translation="MADLVVVGGGPAGVAAALAGRRAGAEVVLIERGLLGGTCVHAGC
                     ISSAAYQPTASVLHDLRRAGALGIELEGGAASAKLSWDRVQAWASRASEAVAAGVRAQ
                     LSYAGVTVERRAA"
     gene            complement(338..1375)
                     /locus_tag="VF995_00190"
     CDS             complement(338..1375)
                     /locus_tag="VF995_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX9342020.1"
                     /translation="MAAAVGLIANPAAGRDIRRLTAHASVVPNHEKASIVRRVLHGLA
                     ATGVDLVLYLADTAGIVAAAVDGARPPVAMEALGLRAHGWASDSTEAARLLAAAGVGA
                     IVTLGGDGTNRAVAAACGEVPLVAVSTGTNNVFPTMVDGTVAGLAAGLVATGAVDAEQ
                     VVRRAKRVEVQVEAAGDAAGGADFALIDAAACTDAFVGTRAVWDAARVRALVLTRAEP
                     WSVGLSAIGGRLCPLAPEDPAGLYVELAPAGEGPAVTATVAPGVVASVAVSGWRLLEL
                     GEPVTLSGLGRGFDAGERRGGARTVALDGERELALDPNTVATATVTLTGPRVVDIRAA
                     LAAAGRGWDRG"
     gene            complement(1384..2637)
                     /locus_tag="VF995_00195"
     CDS             complement(1384..2637)
                     /locus_tag="VF995_00195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX9342021.1"
                     /translation="MLTEVRMPKLGMAMKKGAVTKWLKAEGETVQEGAELFELATEKI
                     TAVVPSPASGLLGRVVAQLGAELPVGALLALIGEPGDTFPPREELAAQPPPVAPAFPI
                     GAPPAPPEAATRAGGRPGDGAPVSPAARRRARELGVDVALVAPSAPGKRVMVEDVEAF
                     AAAAAAVAEAEPEEMPAPAGRVVPFTGIRRVVAERLSESLRTTAQVTISREVEAGGLV
                     TRRAALAAGFEVATGIRLTYTDLLVEAVARLLADHPLLNSTLTEEGIVLPDAVHVGVA
                     VALEDGLIVPVIRDAGTRPLAELARDRAELAAKAQAGTLTLDEVEGGTFTISNLGSFG
                     ADAFTPIINPPQCAILGVGRIVDKPVAVAARVEVRPLLWLSLTFDHRIVDGAPAARFL
                     RELGDRLATPEPAPEPAPEPGPGPG"
     gene            complement(2672..3697)
                     /locus_tag="VF995_00200"
     CDS             complement(2672..3697)
                     /locus_tag="VF995_00200"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX9342022.1"
                     /translation="MAVATRTITFREAVNEALRQEMARDPDVVLLGEDVAGGATVPGF
                     EHEDAWGGVLGVTKGLVQDFGRERVLDTPISESGFIGAAVGAAATGLRPVAELMFVDF
                     FGVCMDQIFNQGAKLRYMFGGKARVPLVIRTMIGAGFRAAGQHSGCHYSVFTHMPGLK
                     TVVPSNPADAKGLLAAAIRDDDPVLFFEHKLLYDTTGEVPEGEHLVELGRAAVPREGD
                     GATVVAIGRMVNLALEAAETLAGAGAGVEVEVIDPRTLSPLDDEAILSSVRKTGRLVV
                     VDEDNPRCSMATDIAALVAAQAFDHLDAPPQLVTAPHTPVPFAPVLEDFYIPTPERIA
                     QAVQATL"
     gene            complement(3713..4699)
                     /locus_tag="VF995_00205"
     CDS             complement(3713..4699)
                     /locus_tag="VF995_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX9342023.1"
                     /translation="MELTQVRELTQDKALWIYDRMVTIRAFEERVAKLFADGQIPGFV
                     HLYAGEEAVAVGVCAHLEDADFITSTHRGHGHCIAKGVPLGAMMAELYGRVDGSCKGK
                     GGSMHIADVSRGMLGANGIVGGGPPLACGAGLTAKLKGSGAVCACFFGDGAAEQGTTH
                     EAMNLAAIWKLPVIFVAENNGFAESTPVGYHCAASSIADRAASYNIPGVSVDGYDAVA
                     MFEAAGEALARARRGDGPSLIEARTWRYFGHFEGDQVTYRTAEQSAAYREHDPLTWFA
                     KQAAERGLLTDAQVEERQRAAEAAVDEAIAFAERSPHPAPTDALSDVYVTYR"
BASE COUNT          618 a         1808 c         1740 g          590 t
ORIGIN      
        1 ggcggcccgc cgctccacag tgaccccggc gtagctgagc tgcgcgcgga ccccggcggc
       61 gaccgcctcg ctggcccgcg acgcccacgc ctggacgcgg tcccagctga gcttggcact
      121 ggcggcaccg ccctccagct cgatcccgag cgccccagcc cgccgcaggt cgtgcaggac
      181 cgaggcggtg ggctggtagg cagccgacga gatgcatccg gcatgcacgc aggtcccacc
      241 gagcaaccca cgctcgatca agacgacctc ggcgcccgcc cgccgcccag ccagcgccgc
      301 cgcgacaccg gccggcccgc cgcccaccac gaccaggtca gccacggtcc cagcctcggc
      361 cggccgccgc gagggcggcg cggatgtcga ccactcgggg gccggtcagg gttaccgtcg
      421 cggtcgcgac cgtgttcggg tcgagggcca gctcccgttc accgtccagg gcaaccgtgc
      481 gcgcgcctcc gcgacgctcg cccgcgtcga agccgcggcc gagcccggag agggtgaccg
      541 gctcgcccag ctccagcagc cgccagccgc tgaccgcgac gctggcgacc acgcccggcg
      601 cgaccgtcgc ggtcaccgcc gggccctccc ccgccggtgc cagctcgacg tacagcccag
      661 cgggatcctc gggcgccagg gggcagaggc ggccgccgat cgcggacagg ccgaccgacc
      721 acggctcggc gcgggtcagc accagcgccc ggaccctggc cgcgtcccag accgcgcggg
      781 tgccgacgaa ggcatcggtg caggcggcgg cgtcgatcag tgcgaagtcg gcacccccgg
      841 cagcgtcccc ggcagcctcg acctggacct cgacgcgctt ggctctgcgt accacctgct
      901 cggcgtctac cgccccggtc gccaccagcc cggcggcgag gccggccacg gtgccgtcga
      961 ccatcgtggg gaagacgttg ttggtcccgg tggagaccgc caccagcggc acctcgccgc
     1021 aggcggcggc caccgcccgg ttggtcccgt cgccgccgag ggtgacgatc gcgccgaccc
     1081 cggccgccgc cagcagccgc gcggcctcgg tcgagtccga cgcccagccg tgcgcccgca
     1141 gcccgagcgc ctccatcgcg accggcgggc gggcgccgtc gaccgccgcg gcgacgatcc
     1201 cggcggtgtc ggccagatac aacaccaggt ccacgccggt cgcggccagg ccgtggagga
     1261 cccgccgcac gatcgaggcc ttctcgtggt tcggcaccac cgaggcgtgg gcggtcaggc
     1321 ggcggatgtc ccgccctgcc gccgggttcg cgatcagccc gaccgccgcc gccacgcccc
     1381 cgcctatccc ggtcccggcc cgggctccgg cgcgggctcc ggcgcgggct ccggcgtggc
     1441 gaggcggtcg cccagttccc gcaggaagcg ggcggcgggc gcgccgtcca cgatccggtg
     1501 gtcgaaggtg agcgacagcc acagcagcgg ccggacctcg acccgggccg cgaccgccac
     1561 cggcttgtcg acgatgcggc cgacgccgag gatcgcgcac tgcggcgggt tgatgatcgg
     1621 ggtgaaggcg tcggcgccga agctgcccag gttcgagatg gtgaaggttc cgccctcgac
     1681 ctcatccagg gtcagcgtcc cggcctgcgc cttggcggcc agctcggccc ggtcgcgggc
     1741 cagctcggcc agcggccggg tgccggcgtc gcggatcacc ggcacgatca gcccgtcctc
     1801 cagcgccacc gccacgccca cgtggaccgc gtcggggagc acgatgcctt cctcggtcag
     1861 ggtcgagttc agcagcgggt ggtccgccag caggcgagcc acggcctcga ccagcaggtc
     1921 ggtgtaggtc aggcggatcc cggtggccac ctcgaacccg gcggcgagcg cggcccgccg
     1981 cgtcaccagc ccgccagcct cgacctcgcg cgagatggtg acctgggcgg tcgtccgcag
     2041 gctctcgctg agccgctcgg cgaccacccg gcggatccca gtgaacggca ccacccgccc
     2101 ggccggagcg ggcatctcct cgggctccgc ctcggcgacg gccgcagcgg cggctgcgaa
     2161 cgcctccacg tcctccacca tgacccgctt ccccggcgcg ctcggcgcta ccagcgcgac
     2221 gtccacgccc agctcccggg cgcggcgccg cgccgccggg gataccgggg cgccgtcgcc
     2281 gggccggccg ccagctcgcg tggctgcctc gggtggagcg ggtggcgccc cgatcgggaa
     2341 cgctggcgcc accggcggcg gctgcgcggc cagctcctcc cggggcggga aggtgtcgcc
     2401 cggctcgccg atgagggcga gcagcgcgcc gaccggcagc tcggcgccga gctgggccac
     2461 cacccggccg agcagcccgg aggccgggga cgggaccacg gcggtgatct tctcggtggc
     2521 cagctcgaac agctcggcgc cttcctggac ggtctcgccc tcggccttga gccacttggt
     2581 gaccgcgccc ttcttcatcg ccatgccgag cttcggcatg cggacctcgg tgagcatcag
     2641 cgggacctcg ctcgctcgcg actcgcgacc actacagggt tgcctggacc gcctgggcga
     2701 tgcgctccgg cgtcgggatg tagaagtcct ccagcaccgg cgcgaacggc accggcgtgt
     2761 gcggtgccgt gaccagctgc ggcggggcat ccaggtggtc gaaggcctgg gcggcgacca
     2821 gcgcggcgat gtcggtggcc atcgagcagc gcgggttgtc ctcgtcgacc accaccaggc
     2881 ggccggtctt gcgcaccgac gaaaggatcg cctcgtcgtc cagcggcgac agcgtgcgcg
     2941 ggtcgatcac ctccacctcg acgccggcgc cggcgccggc cagcgtctcg gccgcctcca
     3001 gcgccaggtt gaccatccgg ccgatggcga ccacggtggc gccgtcgccc tcgcggggca
     3061 cggcggcacg gcccagctcg acgaggtgct cgccctccgg gacctcgccg gtggtgtcgt
     3121 agagcagctt gtgctcgaag aacagcaccg ggtcgtcgtc gcggatcgcg gcggcgagca
     3181 ggcccttggc gtcggcgggg ttggacggca ccaccgtctt gaggccgggc atgtgggtga
     3241 agaccgagta gtggcagccg gagtgctgcc cggccgcgcg gaacccggcg ccgatcatgg
     3301 tgcggatcac cagcgggacc cgcgccttgc cgccgaacat gtagcgcagc ttggcgccct
     3361 ggttgaagat ctggtccatg cagacgccga agaagtcgac gaacatcagc tcggcgaccg
     3421 ggcgcagccc ggtggcggcg gcgccgaccg ccgcgccgat gaagcccgac tcggagatcg
     3481 gcgtgtcgag cacgcgttcg cggccgaagt cctgcaccag gcccttggtg acgccgagga
     3541 cgccgcccca ggcgtcctcg tgctcgaacc cggggacggt ggcgccgccg gcgacgtcct
     3601 cgcccagcag caccacgtcg gggtcgcggg ccatctcctg gcgcagcgcc tcgttgacgg
     3661 cctcgcggaa ggtgatcgtg cgggtggcga cggccatggg agcaccctcc tcctaccggt
     3721 aggtgacgta gacgtcgctg agcgcgtcgg tgggggcggg atgggggctc cgctcagcga
     3781 aggcgatcgc ctcgtccacg gcggcttcgg cggcccgctg gcgctcctcg acctgggcgt
     3841 cggtgagcag cccgcgctcc gccgcctgct tggcgaacca ggtgagcgga tcgtgctcgc
     3901 ggtaggcggc cgactgctcg gcggtccggt aggtgacctg gtcgccttcg aaatgcccga
     3961 agtagcgcca ggtcctggcc tcgatcaggc tgggcccgtc gccccggcgg gcgcgggcga
     4021 gcgcctcgcc ggccgcctcg aacatggcga cggcgtcgta gccgtcgacg gagacgccgg
     4081 ggatgttata agaggcggcg cggtcggcga tgctggaggc cgcgcagtgg tacccgaccg
     4141 gggtggattc ggcgaagccg ttgttttcgg cgacgaagat caccgggagc ttccagatcg
     4201 cagccaggtt catcgcctcg tgggtggtgc cctgctcggc cgcgccgtcg ccgaagaagc
     4261 aggcgcagac cgcgccgctg cccttgagct tggcggtgag cccggcgccg caggccagcg
     4321 gcgggccgcc gccgacgatc ccgttggcgc ccagcatgcc ccgggagacg tcggcgatgt
     4381 gcatcgagcc gcccttgccc ttgcaggagc cgtccacccg cccgtacagc tcggccatca
     4441 tggcgccgag cgggacgccc ttggcgatgc agtggccgtg gccccggtgg gtggaggtga
     4501 tgaagtcggc gtcctccagg tgggcgcaga cgccgaccgc gaccgcctcc tcaccggcgt
     4561 aaaggtggac gaagccaggg atctggccgt cggcgaacag cttcgccacc cgctcctcga
     4621 aggcccggat ggtcaccatg cgatcgtaga tccagagggc cttgtcctgg gtcagctccc
     4681 tgacctgggt cagctccatc gcgtccctcc cgggctgcat ctggacaatc ctggcaccca
     4741 ggcaagagcc tacgcc
//
LOCUS       DASWDC010000009         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_669247, whole genome shotgun sequence.
ACCESSION   DASWDC010000009 DASWDC010000000
VERSION     DASWDC010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669247"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VF995_00210"
     CDS             <1..451
                     /locus_tag="VF995_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342024.1"
                     /translation="AARTLVAVQVSLVVGGSAALIALGPRATRMVFGDGFSMSRGELL
                     AVAVAMLAQTACEVSLSRLRSVGKVRTAMFGRILVTVVAVAAAYAVGTSLVGVMVALL
                     VSQLAGAGFAFAARTHDAGREADVRVGPARFGLLRRVVMAAFAALHQ"
     gene            complement(560..1369)
                     /locus_tag="VF995_00215"
     CDS             complement(560..1369)
                     /locus_tag="VF995_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX9342025.1"
                     /translation="MKPLEAGERVLLQDSKGRRYLVTLQAGATFHTHRGRLAHDQLIG
                     SREGEVAATDLGQRLLVLRPTLADWVLKMPRGAQVIYPRVLALMVMAADVQPGMTVLE
                     AGAGSGALSMTLLQALGPSGRLVSFELRPEFAAQARRNVEAWFGKLPDAWDLRDGDVV
                     EGIATVGTVDRVLLDLLEPWLVVPGAAGALEPGGVLVAFVATVPQVMRLVEALDTSRQ
                     FGLVETSEAILRPWHVDGLAVRPEHRMIGHTGFLVSARRVSVPWGPPAPVR"
     gene            complement(1366..2349)
                     /locus_tag="VF995_00220"
     CDS             complement(1366..2349)
                     /locus_tag="VF995_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9342026.1"
                     /translation="MAMRCKRCRGPVVIEVRRHNAAFCRDCFVHYAGQQVTRAIAKHH
                     MLAPTDRILVAVSGGKDSLALWDILLGLGYQTDGLYLSLGIGAYSERSKQLCERFAAA
                     RGATLIVHDLANEQGYTIPQAAAQAARAGTAGRSSCGVCGLSKRYIFNKAALDGGYDV
                     IATGHNLDDEAAVLFGNTLRWDTESMARQYPVLEATRPGLVKKVKPLYRLAERETAAY
                     CVLKAIDYVIEECPLVAGNTQMRHKETLNQMEEASPGTKHNFLFGYLDRAAALLRRAD
                     GAELAECSRCGMVTQAPDDPAEAPVCAFCRTRARLVDGDARPLPITEVRAG"
     gene            complement(2356..2559)
                     /locus_tag="VF995_00225"
     CDS             complement(2356..2559)
                     /locus_tag="VF995_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX9342027.1"
                     /translation="MLVTARLRPPDRTVEVADVERVADLLAKLEVLQGTVLVIRDGDL
                     LTSQDRLRDGDSVEVRFAISGGA"
BASE COUNT          358 a          996 c         1079 g          421 t
ORIGIN      
        1 ggcggcccgc acgctggtcg ccgtgcaggt cagcctggtg gtcggcggca gcgcggcgct
       61 gatcgcgctg ggcccgcgcg ccaccaggat ggtattcggc gacggcttct cgatgagccg
      121 tggcgagctg ctggcggtcg cggtggcgat gctcgcccag accgcctgcg aggtgagctt
      181 gtcgcggctg cggtcggtcg gcaaggtccg cacggcgatg ttcggccgga tcctggtcac
      241 cgtggtcgcg gtcgccgcgg cctacgcggt cggcacctcg ctggtcgggg tgatggtcgc
      301 gctgctggtg agccaactcg ccggcgctgg cttcgcgttc gccgccagga cccacgacgc
      361 tggccgggaa gcggatgtca gggttggccc ggcccgtttc ggcctgctcc gccgggtggt
      421 gatggccgcc ttcgcggcgc tgcaccagta gctcggctgg ttgtgtcgag ggcgcgcggc
      481 cggactcccc cgggtggtcc aggccgcgcg ccctcgacct gtattcgccg ctggatcacg
      541 cggcggttca gcggcggcgt cagcggactg gggcgggcgg tccccagggg acgctgaccc
      601 ggcgggcgga gaccaggaag ccggtgtggc cgatcatgcg atgctccggg cggaccgcga
      661 ggccgtcgac gtgccagggc cgcaggatcg cctctgaggt ctcgaccagg ccgaactgcc
      721 gcgaggtgtc cagcgcctcc accaggcgca tcacctgcgg gacggtggcg acgaaggcga
      781 ccagcacccc gccgggttcc agcgccccgg cggcgccggg gaccaccagc cacggctcca
      841 gcaggtcgag cagcacccgg tcgacggtgc cgacggtggc gatcccctcc accacgtcgc
      901 cgtcgcgcag gtcccaggcg tcggggagct tgccgaacca ggcctcgacg ttgcggcggg
      961 cctgggcggc gaactcgggg cgcagctcga aggagaccag ccgcccgctg ggcccgaggg
     1021 cctgcagcag cgtcatcgac agggcccccg acccggcccc ggcctccagc acggtcatgc
     1081 cgggctggac gtcggcggcc atcaccatga gggcgagcac ccgcgggtag atcacctggg
     1141 cgccccgggg catcttcagc acccagtcgg ccagggtcgg ccgcagcacc agcagccgct
     1201 ggccgaggtc ggtggcggcc acctccccct cgcggctgcc gatcagctgg tcgtgggcga
     1261 ggcgcccgcg gtgggtgtgg aaggtcgcgc cggcctgcag cgtcaccaga tagcggcggc
     1321 ccttgctgtc ctgcagcagc acccgctcgc ccgcctccag gggtttcacc cggcgcgcac
     1381 ctccgtgatc ggcagcggcc gggcgtcccc gtcgaccagg cgggcgcggg tgcggcagaa
     1441 ggcgcagacg ggcgcctcgg cggggtcgtc gggcgcctgg gtgaccatgc cgcagcggga
     1501 gcactcggcc agctcggcgc cgtcggcgcg gcgcagcagc gcggcggccc tgtcgaggta
     1561 gccgaacagg aagttgtgct tggtgcctgg ggaggcctcc tccatctggt tcagggtctc
     1621 cttgtggcgc atctgggtgt tgccggcgac cagggggcat tcctcgatca cgtagtcgat
     1681 ggccttgagg acgcagtagg cggcggtctc gcgctcggcc aggcggtaca gcggcttgac
     1741 cttcttgacc aggccggggc gggtcgcctc cagcaccggg tactggcgcg ccatcgactc
     1801 ggtgtcccag cgcagggtgt tgccgaacag caccgcggcc tcgtcgtcga ggttgtggcc
     1861 ggtggcgatc acgtcgtagc cgccgtcgag ggcggccttg ttgaagatgt agcgcttgga
     1921 cagcccgcag accccgcagg aggagcgccc ggccgtgccc gccctggccg cctgcgcggc
     1981 ggcctgcggg atggtgtagc cctgctcgtt ggcgaggtcg tggacgatca gcgtggcgcc
     2041 gcgtgcggcg gcgaagcgct cgcagagctg cttggaacgc tcggagtagg cgccgatccc
     2101 aaggctgagg tagagcccgt cagtctggta gccgaggccg aggaggatgt cccagagtgc
     2161 cagggagtcc ttgccgccgg agaccgcgac caggatccgg tcggtgggtg ccagcatgtg
     2221 gtgcttggcg atcgcgcggg tgacctgctg gccggcgtag tggacgaagc agtcccggca
     2281 gaaggccgcg ttgtggcggc ggacctcgat caccaccggg ccgcggcagc gtttgcagcg
     2341 catcgccatc gccgctcacg cccctccgga gatggcgaag cggacttcga cgctgtcccc
     2401 gtcgcggagc cggtcctggc tggtgagcag gtcgccgtcg cggatcacca gcacggtccc
     2461 ctggaggacc tccagcttgg cgagcaggtc ggcgacccgt tcgacgtcgg cgacctcaac
     2521 ggtccggtcg ggcggccgca gccgcgcggt cacgagcacg gagtccgcct cctcctcctt
     2581 ggtcggctga atcttgggct gctcaattgg gagattccga agacctcgaa gccaacgata
     2641 ccgccgcgtg acccccctcg ctcgcgcggc gcggcgcggc ttacactggt ttgcccgcct
     2701 ttcctgtcga gcgagatcga ggctgtcatg ggtgctcccg ccgtgactcc aaacgacccg
     2761 gaccatcccg ctgagattga cgacggcgac atcccggccc cggccccgct gctggtcacg
     2821 tccctgcacg gcctctgggg tggccgcggc cgtc
//
LOCUS       DASWDC010000010        12147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_669286, whole genome shotgun sequence.
ACCESSION   DASWDC010000010 DASWDC010000000
VERSION     DASWDC010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12147
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669286"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..866)
                     /locus_tag="VF995_00230"
     CDS             complement(48..866)
                     /locus_tag="VF995_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX9342028.1"
                     /translation="MTAPSDPFEVVLLDLDGTVYIGHLPVPGSPEAIAALRARGQRVA
                     FLTNDPAAGRGEFAARLATIGVPATPDDVVTTGMALALLLRQEGFEGATALILGTPAF
                     RTEVMAAGLLDADATGADPKSVDLVVVGGSVDLGHAELTRATRALRAGARLFGAGRDA
                     TYPTPSGPAPASGPYLASVEVAGGVPATVAGKPERPIFDAARTLLGATDGTPICMVGD
                     RLDSDILGAHRAGLTAILVLSGTTTPTDAATAPITPDLICHNLAEFTGVTDRGD"
     gene            complement(868..2034)
                     /gene="gcvT"
                     /locus_tag="VF995_00235"
     CDS             complement(868..2034)
                     /gene="gcvT"
                     /locus_tag="VF995_00235"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX9342029.1"
                     /translation="MTPATDAEAGSAEAGSAAEPEAGLRRSPLDAVHRRLRAKMGAFA
                     GWDMPIEYAGTLSEHRAVREAVGVFDLSHLGKVEVRGPGAYDTLQHALSNDLDRLTEE
                     GAAQYTLCLSDDGGIVDDLIAYREADGYLLVPNAANWPAVADAVRASAHRGDTEVTPR
                     PDLAVIAVQGPAAPELLDGLFEEADGLPYMHQAPITWSGATARLCRTGYTGERGYELI
                     VPGERAPALWDELFDRGEQLGAVPCGLGARDTLRLEMGYPLHGNDISTATDPFMARLG
                     WAVRMDKPAFRGKPALLERKQSGPERLLVGLVATDRLIPRHGMAVLDPDDPDGRPIGE
                     VTSGTFSPTLRHGIALAYVVPAFAKDGTGLLVDVRGRRCSTRVTRPPFVDAAAK"
     gene            complement(2214..2480)
                     /locus_tag="VF995_00240"
                     /pseudo
     CDS             complement(2214..2480)
                     /locus_tag="VF995_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785383.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2551..2997)
                     /locus_tag="VF995_00245"
     CDS             complement(2551..2997)
                     /locus_tag="VF995_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9342030.1"
                     /translation="MEISEIMTKEVVTVGPEYNVADVASMMHARGIGSVIVLEDDRVL
                     GILTERDILRVVGSGEDPKTVAAHEALTDDLITVSPSAAVEEAAREMVRAGVRHLPVV
                     ADEGLIGIVSIRDLVRWSVQGMAESTELPHIEVSQQVLSLVHKADT"
     gene            3167..4576
                     /gene="lpdA"
                     /locus_tag="VF995_00250"
     CDS             3167..4576
                     /gene="lpdA"
                     /locus_tag="VF995_00250"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9342031.1"
                     /translation="MPSDSYDLVILGGGTGGYSAALRGAGLGLSVALVEKDKVGGTCL
                     HRGCIPTKALLHAAELVDGVHEAAERWGIKTAIESIDYAATVANREDIVNKNFKGLEG
                     HLKKEKVEILAGNGRVTGPKQVEVEGVGTITANKALVLASGSVPKSLPGLEIDGQRVI
                     TSDHALQLDYLPKSVIVLGAGAVGVEFASFYRSMGADVTVVEVLPGLVPLEDADIQKE
                     LGRAFRKRGIDVLVDTRFEEAKVDGDQVRVTVTSKGGKGDSKELTAELLLVATGRGPV
                     SAGLGYEQIGVRLDRGYVVPKNWDTLETDAAGVYAVGDLLPPPSLALAHASFAEGMLV
                     AELAAGLPSPPIDYAGVARVTFSTPEVASIGLTEAQARDQGYEVVTNRFPFNGVAKGM
                     IHGQGGMVKLVAAKDGPVLGVHLVGARVTELVAEAMLVYNWEALPSDVAQFIHPHPTL
                     SEAVGEAYLTLAGRPLHQG"
     gene            4604..6034
                     /locus_tag="VF995_00255"
     CDS             4604..6034
                     /locus_tag="VF995_00255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX9342032.1"
                     /translation="MATPVKLPELGESITEGTITRWLKQEGDHVAVDEPLYEVSTDKV
                     DTEVPAPVAGVLRSIKVKEDETVAVGTELAVIEQDGEAGGDGGGARAKAEAEAEPEQA
                     AAQPQAEREAPAEAEAAPAAEARRESAPSPAPAQAPVAVERDGERREGRGGGRATASP
                     LVRRLAREQGVDLSQVAGTGPGGRVRREDIDAFLDQRGQKPAAAKAPATEAPAAAAAP
                     QPQVAVPLAQGLREEVVRSTRLRRLIAERMVSSLQTSAQLTAAVEVDMTRVMRLRERA
                     KDQFKQREGLSLSPLPFCVMALIAAVKQYPAVNSALDEDGNLHIYQDIHLGVAVDTPK
                     GLFVPVIKGADSLNLVGLTRAIADLARRTRDGSIRPDELSGSTITVTNTGSRGTVWDT
                     PIINQPNAAIFATPAIVKRPWVIEDPDLGEVIAVRHIMEAILTYDHRIIDGADAARFL
                     GTIKAVLESDDFTAELAAELGVKNQG"
     gene            6048..6758
                     /gene="lipB"
                     /locus_tag="VF995_00260"
     CDS             6048..6758
                     /gene="lipB"
                     /locus_tag="VF995_00260"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX9342033.1"
                     /translation="MEPLWTVRAGVVPYRRAWAWQRALVERRVAGAIPDTVLLLEHPH
                     VYTIGKRGADADVLVSEEWLAAQGAEVVHSDRGGQVTYHGPGQLVGYPITLLEPALGV
                     WPFIRRVEEALCDVAAGFGLQARGEGGLLTGVWVESGAIDAKLAAIGMRVSRGVTSHG
                     FALNCDTDLSKFNAIVPCGMPDKPACSLSSLLGRQVTVAEVLPLAERHLAERLGRKPV
                     PVDLATLELPEPDPAAVT"
     gene            6774..7739
                     /gene="lipA"
                     /locus_tag="VF995_00265"
     CDS             6774..7739
                     /gene="lipA"
                     /locus_tag="VF995_00265"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX9342034.1"
                     /translation="MPEPVIPAGVRTLSVLPRSPAPPPTEQTFARKPSWIRVKARSGP
                     NYQELQGLVRDEGLHTVCQEAGCPNIYECWEDREATFLIGGDNCTRSCPFCQIHTGKP
                     EGYDTDEPRRVAHAIARMGLNFAVITGVARDDLADGGAWLFAESIRQVRALLPSCGVE
                     VLIPDFRGSPEALRQVVDATPDVLGHNVETVPRLYKRIRPGFTYAGSLELLARARAWL
                     PQGSATKSNLILGMGEERDEVNQTMRHLREVGVELLTITQYLQPSRQHLNLQRFVTPE
                     EFAELKAEAEGLGFAHVESGPLVRSSYHAGRMHEAATGAARAATR"
     gene            7736..8281
                     /locus_tag="VF995_00270"
     CDS             7736..8281
                     /locus_tag="VF995_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342035.1"
                     /translation="MTASALHLGRPGRRARVTVAAVLLVALVGWLGWRAQQAFDHSRH
                     LAAARAVVSAPDVVTYRLPASGVGSRVSGMLQYRRGEWLSVVTLHGLSPVGGREHYLV
                     FLHGFSGWTLAGAVRPNPDGSAQVRFGAEPRPSSTYEVVVTRGVDDATSIPHGTMLLH
                     WFDVGLGPLRVKPFDPAHPVP"
     gene            8486..9637
                     /locus_tag="VF995_00275"
     CDS             8486..9637
                     /locus_tag="VF995_00275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9342036.1"
                     /translation="MTRTALDLRRPRVALVASPRATLPPGSYGGSVEQVTGLAAALAR
                     RGYEITVIGAGLTIAPGASRYRAIDTQQDEGLTDPVLAEALHGVAVERLLRRLDVDLV
                     HDHTVIGMLTPASDRDIPRIVTVYTPTSDADGVPSAKVGGRVGGGAALVAVSGHQRAQ
                     APGLPWLGVVHPGILVTEYPYREEKDGSCLFVGALAPGRGADLAIAAAHAAGRSITVA
                     GTLPQPGMATYVQWRLRPLLRPGDKLIVAADHAKRTSLLLHARCLVAPFGADVPFSLP
                     VVEALACGTPVVALRGGVAEELVEHGVSGILCPTPAELAEGIAAAERLDPADCRRHAA
                     ARFDTTQMAAGYDRLYSQALAARLQQTPAASPDPHPHRAPVPAGGRSWP"
     gene            9791..10852
                     /locus_tag="VF995_00280"
     CDS             9791..10852
                     /locus_tag="VF995_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX9342037.1"
                     /translation="MRRSRFLLLLTISAALLPLALPAAGLAPALARETPATGANAAAF
                     TATATGQVFDPNPVVTLQDETLTDQQDKDYPALNPAYRTVTLTRLDGSGYLHGDFATI
                     DPKQKTPAFSSTGQFVYLRSDDRFEQVMAYYHVTAAQTYIQSLGFTTINNEPQNLLLN
                     PIGQDNSYYDPKTDIITFGKGGVDDAEDAEMIWHEYGHAIQDSQVPGFGSGNQAGSIG
                     EGFGDYWAFTMSQADSPDTATTPLACIADWDSTAYTYNQLPHCLRRVDTNKTMADYQP
                     NGDVHVNGQIWSRALHDINLALGRDPANTVILQAQFAFSPSTTFQAAAQATVDTATAL
                     YGSATASQVQAAFAARGIL"
     gene            11071..>12147
                     /gene="glnA"
                     /locus_tag="VF995_00285"
     CDS             11071..>12147
                     /gene="glnA"
                     /locus_tag="VF995_00285"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX9342038.1"
                     /translation="MTDSVGDVLKLIKDRGVQIIDLRFADLPGLTQHFSVPPSELTEA
                     SFEEGFGFDGSSIRGFQEIQESDMLLFPDPGTAVLDPFAKHTTLVLNCFVGDPVTGEP
                     YSRDPRHIARKAEAYLKGTGIAETSYWGPECEFYIFDSVRFDQNQYSAYHFIDAIEGQ
                     WNSGAEEGGHNLGYKPRYKEGYFPVPPTDHYQDIRSEMVLKMIEAGIGIEVHHHEVGT
                     AGQAEIDMRFDTLLRMADKVMLYKYIVKNVARAWGKTATFMPKPIFEDNGSGMHTHQS
                     LWTNGEPLFYDESGYAGLSDTGRWYIGGLLAHAPALTALSNPTTNSYRRLVPGYEAPV
                     NLVYSQRNRSAACRIPLYSKSPKAK"
BASE COUNT         1830 a         4254 c         4373 g         1690 t
ORIGIN      
        1 ctcgcgacac tcccgtcaag cacgcccgat gcggtcacac ccacccatta gtctccccgg
       61 tcggtgaccc cggtgaattc ggcgaggttg tggcagatga ggtcgggggt gatgggggcg
      121 gtggcggcgt cggtgggggt ggtggtgccg gagaggacga ggatggcggt gaggccggcg
      181 cggtgggcgc cgaggatgtc ggagtcgagg cggtcgccga ccatgcagat gggggtgccg
      241 tcggtggcgc cgaggagggt gcgggcggcg tcgaagatgg ggcgttcggg tttgccggcg
      301 acggtggcgg ggacaccgcc ggcgacctcg accgaggcga ggtaggggcc ggaggcgggg
      361 gcggggccgg acggggtcgg gtaggtggcg tcgcggccgg cgccgaacag gcgggcgccg
      421 gcgcgcaggg cccgcgtcgc gcgggtcagc tcggcgtggc cgaggtcgac tgagccgccg
      481 accaccacca ggtcgaccga cttcgggtcg gctccggtgg cgtcggcgtc caagagcccg
      541 gcggccatga cctcggtgcg gaaggctggc gtgccgagga tcagcgcggt agcgccctcg
      601 aagccctcct ggcgcagcag cagggccagg gccatgccag tggtgaccac gtcgtcggga
      661 gtggcgggga cgccgatggt ggcgaggcgg gcggcgaact cgccacggcc cgcggccggg
      721 tcgttggtga ggaaggcgac ccgctggccg cgcgcccgca gcgccgcgat cgcctccggg
      781 gacccgggca ccggcagatg gccgatgtag acggtcccat cgagatcgag caggacgacc
      841 tcgaacgggt cggagggagc cgtcacgtca cttcgcggcg gcgtcgacga agggcgggcg
      901 agtgacgcgg gtgctgcagc ggcggccgcg gacgtcgacc agcagcccgg tgccgtcctt
      961 ggcgaatgcg gggacgacgt aggcgagggc gatgccgtgg cgcagggtcg gcgagaaggt
     1021 gccgctggtc acctccccta tcgggcggcc gtcggggtcg tcggggtcga ggacggccat
     1081 gccgtggcgt gggatgaggc ggtcggtggc gaccaggccg acgaggaggc gctcggggcc
     1141 ggactgcttg cgctccagca gggcgggctt gccgcggaag gcaggcttgt ccatgcggac
     1201 ggcccagccg aggcgggcca tgaaggggtc ggtggcggtg gagatgtcgt tgccgtgcag
     1261 ggggtagccc atctcaaggc gcagggtgtc gcgggcgccg aggccgcagg ggaccgcgcc
     1321 gagctgctca ccgcggtcga acagctcgtc ccacagggcg ggcgcgcgct cgccgggaac
     1381 gatcagctcg tagccgcgct cgccggtgta gccggtacgg cacaggcggg cggtcgcgcc
     1441 gctccaggtg atgggcgcct ggtgcatgta gggcaggccg tcggcctcct cgaacaggcc
     1501 gtcgagcagt tcgggggcgg ccgggccctg caccgcgatg acggcgaggt cggggcgtgg
     1561 ggtgacctcg gtgtcgcctc ggtgggcgga ggcgcgcacc gcgtcggcca ccgccggcca
     1621 gttggcggcg ttggggacca gcaggtagcc gtcggcctcg cggtaggcga tcaggtcgtc
     1681 gacgatgccc ccgtcgtcgc tgaggcagag ggtgtactgg gccgcgccct cctcggtgag
     1741 gcggtccagg tcgttggaga gggcgtgctg cagggtgtcg taggcgcccg ggccgcggac
     1801 ctcgaccttg ccgaggtggg aaagatcgaa gacgcccacc gcctcacgga ccgcgcggtg
     1861 ctcgctgaga gtgccggcgt actcgatcgg catgtcccag ccggcgaacg cgcccatctt
     1921 ggcgcgcagg cggcggtgga cggcgtccag cggcgagcgc cgcagcccgg cctcgggctc
     1981 cgccgctgac ccggcctcgg ctgacccagc ctcggcgtcg gtcgccggtg tcactcctgc
     2041 tcccctgcct caggagcggg aggggtcggc ggtcccggag gggtccgggg aaccccaggg
     2101 gggtgcccag gtgaatcaat tctctgtggc cgggggatgc ggccgggcgg ctgggaggtg
     2161 cgcaccggtg gggcgccggg ctgctgcagg cgccggtcgg cgcggccctc ggctttgagg
     2221 cgggcgttgt aggcgcgcat gcggtcgggg tagccgacga tctgcacgtc gtagacgggg
     2281 atctccagct cgtgggcgag gcggcgggcg accttgggcg agccgacccg gcggcgggtc
     2341 cattcgccgt cggcagcgat ggccatggcg gtggtgtcgg tgaccatggt ctcgggctcc
     2401 acgtagaact cgacgccggt gcgggtgcgg acgaactcgc ggagcgcgtc gaggtcctcg
     2461 gaggtggcct cccggtccat gctggcgacc tgggtgccac ggctgcggcg gatgcggagt
     2521 gcgtcgagga atttgctcat caggtcgggc tcacgtgtcg gctttgtgga caagggagag
     2581 gacctgctgc gaaacctcga tatggggcag ctcggtcgac tcggccatac cttggacgct
     2641 ccagcggacc aggtcgcgga tggagacgat gccgatcagg ccctcgtcgg cgacgaccgg
     2701 caggtgccgg acgccggcgc ggaccatctc gcgggcggcc tcctccaccg cagcggacgg
     2761 gctgacggtg atgagatcgt cggtgagggc ctcgtgcgcg gccacggtct tgggatcctc
     2821 gccggagccg accacccgga ggatgtcgcg ctcagtgagg atgccgagga cccggtcgtc
     2881 ctccagcacg atcaccgagc cgatcccccg ggcatgcatc atgctcgcca cgtcggccac
     2941 gttgtattcc gggccgacgg tgacgacctc cttggtcatg atctcggaga tctccacgag
     3001 gcacccctcc ttgcgtgcac caaaggttca ggcggcgacc ccaaggccgg tagcctggac
     3061 tccgagatta tcgtaggccg ttagctgcgg ccatgacggg aggcgcgtcg ctagactggc
     3121 gcctcggaag cccctgctgg cgatatccca aaaggagagt ttgcgaatgc cctcggattc
     3181 ctatgacctg gtgatcctgg gtgggggcac cggtgggtac tcggctgccc tgcgtggcgc
     3241 gggcctcggg ctcagcgtcg ctctggtcga aaaggacaaa gtgggcggca cctgcctgca
     3301 ccggggctgc atccccacca aggccctgct ccacgccgca gaactggtgg acggcgtcca
     3361 cgaggcggcg gagcggtggg gcatcaagac cgcgatcgag tcgatcgact acgccgcgac
     3421 ggtggccaac cgagaggaca tcgtcaacaa gaacttcaaa ggcctggaag gccacctaaa
     3481 aaaagaaaag gtggagatcc tggccggcaa cggcagggtg accggcccca agcaggtcga
     3541 ggtcgaaggc gtgggcacga tcaccgccaa caaggcgctg gtgctggcct ccggatcggt
     3601 cccgaagtcg cttcccggcc tggagatcga cgggcagcgg gtgatcacct ccgatcacgc
     3661 gttacagctc gactacctgc cgaaatcggt gatcgtgctg ggcgcgggcg cggtcggcgt
     3721 cgaattcgcc tccttctacc gctccatggg cgccgacgtg acagtggtgg aggtcctacc
     3781 cggcctggtg ccgctggagg acgccgacat ccagaaggag ctggggcggg cgttccgcaa
     3841 acgcggcatc gacgtgctgg tggacacccg cttcgaggag gccaaggtcg acggcgacca
     3901 ggtccgcgtc accgtcacca gcaagggcgg caaaggcgac tccaaggagc tgaccgccga
     3961 gctgctgctg gtcgccaccg gccgcgggcc ggtcagcgcc ggtctcggct acgagcagat
     4021 cggcgtcagg ctcgaccgcg gctatgtggt ccccaagaac tgggacaccc tggagaccga
     4081 cgcggccggc gtctacgcgg tcggcgacct cctgccgccg ccgtcgctgg cgctggcgca
     4141 cgcctccttc gccgaaggga tgctggtggc ggagctagcc gccggactgc cctcgccgcc
     4201 gatcgactac gcgggcgtgg cgcgggtgac cttctccacc ccggaggtcg cttcgatcgg
     4261 cctcaccgag gcgcaggcgc gcgaccaggg ctacgaggtg gtcaccaacc ggttcccgtt
     4321 caacggggtc gccaagggca tgatccacgg ccagggcggc atggtgaagc tggtcgcggc
     4381 caaggacggc ccggtgctcg gcgtgcacct ggtgggcgcg cgggtcaccg agctggttgc
     4441 cgaggcgatg ctggtctaca actgggaggc gctgccgagc gacgtcgccc agttcatcca
     4501 cccgcatccg accctgtcgg aggcggttgg ggaagcctac ctgaccctgg ccggccggcc
     4561 gctgcaccag ggctgaccat cgagtctgaa aggaagcgac gacatggcca cgccagtcaa
     4621 gttgccggag ctgggcgagt cgatcaccga gggcaccatc acccgctggc tcaaacagga
     4681 aggcgaccac gtcgcggtgg acgagccgct gtacgaggtc tccaccgaca aggtcgacac
     4741 ggaggtcccc gccccggtcg ccggcgtgct gcggtccatc aaggtcaagg aggacgagac
     4801 cgtcgcggtc ggcacggagc tggccgtaat cgagcaggac ggcgaggctg gtggcgacgg
     4861 cggcggtgct cgtgccaagg ctgaggctga ggcggagcct gagcaggccg cagctcagcc
     4921 tcaggctgag cgtgaggcgc cggccgaggc ggaagcggcg ccggcagccg aggctcggcg
     4981 cgagtctgcg ccctcccccg ctccggccca ggcacctgtg gccgtggagc gcgacggcga
     5041 acggcgcgag ggccgaggag gtggcagggc gacggcgtcg ccgctggtgc gccggctggc
     5101 gcgcgaacag ggcgtggacc tgagccaggt ggcgggcact ggtcctggcg gacgggtgcg
     5161 gcgcgaggac atcgacgcct tcctcgacca gcgcggccag aagccggcgg cggccaaggc
     5221 cccggcgacg gaggcgcccg cggcggcagc ggcaccccag ccccaggtcg cggtgccgct
     5281 ggcgcagggg ttgcgtgagg aagtggtgcg ctcgacccgg ctgcggcgcc tgatcgccga
     5341 gcggatggtc agctcgctgc agaccagcgc ccagctcacc gccgcggtcg aggtcgacat
     5401 gacccgggtg atgcggttgc gcgagcgcgc caaggaccag ttcaagcagc gtgagggcct
     5461 gtcgctgtcg ccgctgccgt tctgcgtcat ggcgctgatc gccgcggtca agcagtaccc
     5521 cgccgtcaac tcggccctgg acgaggacgg caacctgcac atctaccagg acatccacct
     5581 cggcgtcgcg gtcgacaccc ctaaaggcct gttcgtgccg gtgatcaagg gggcggacag
     5641 ccttaacctg gtcggcctga cccgggcgat cgcggacctg gcgcggcgca cccgcgacgg
     5701 cagcatcagg ccggacgagc tgtccgggtc caccatcacg gtgaccaaca ccggcagccg
     5761 cggcactgtc tgggacacgc caatcatcaa ccagcccaac gcggccatct tcgccacccc
     5821 ggcgatcgtc aagcggccgt gggtgatcga ggacccggac ctcggcgagg tcatcgccgt
     5881 gcgccacatc atggaggcga tcctcaccta cgaccaccgc atcatcgacg gcgccgacgc
     5941 ggcccgcttc ctgggcacga tcaaagcggt cctggaaagc gacgacttca ccgccgagct
     6001 ggccgcggag cttggcgtca agaaccaggg ttgagggggt ccctccgatg gagccgctgt
     6061 ggacggtccg ggccggggtg gtgccctacc ggcgtgcctg ggcgtggcag cgggcgctgg
     6121 tcgagcggcg cgtcgccggg gccatccccg acaccgtgct gctgctggag catccgcacg
     6181 tctacaccat cggcaagcgc ggcgccgacg cggacgtgct ggtaagcgag gagtggctgg
     6241 ccgcccaggg cgccgaggtg gtccacagcg accggggtgg ccaggtcacc taccacgggc
     6301 cgggccagct ggtcggctac ccgatcaccc tgttggagcc ggcgctgggc gtgtggccgt
     6361 tcatccgccg ggtcgaggag gccctctgtg acgtcgccgc cggcttcggc ctccaggctc
     6421 gtggcgaggg cgggttgctc accggggtgt gggtggaatc gggagccatt gacgcgaagc
     6481 tggccgccat cggcatgcgc gtgtcgcgcg gggtgaccag ccacggcttc gccctcaact
     6541 gcgacacgga cctgtccaag ttcaacgcga tcgtcccgtg tgggatgcct gacaagcccg
     6601 cctgctcact gtcgagcctg ctcggccgcc aggtcaccgt cgccgaagtg ctgccgctgg
     6661 ccgaacgcca cctcgccgaa cggctcgggc ggaagcccgt cccggtggac ctcgctacgc
     6721 tggaactgcc agaacccgac cccgccgccg tcacgtaagt ccaggtgcac agcatgcccg
     6781 aacccgtcat ccccgctgga gtcaggaccc tgtcagtgct gccgcgcagc ccggcgccac
     6841 caccgaccga gcagaccttc gcgcgcaaac cctcctggat ccgggtcaag gcccgctccg
     6901 gccccaacta ccaggagctg cagggcctgg tgcgggacga gggcctgcac accgtctgcc
     6961 aggaggccgg ctgccccaac atctacgagt gctgggagga ccgcgaggcg accttcctga
     7021 tcggcgggga caactgcacc cggtcctgcc cgttctgcca gatccacacg ggcaagcccg
     7081 agggctacga caccgacgag ccccgccggg tcgcccacgc gatcgcccgc atgggcctca
     7141 acttcgcggt catcaccggg gtcgcccgcg acgacctggc cgacggcggc gcgtggctgt
     7201 tcgccgagtc gatccggcag gtccgggcgc tgctgccaag ctgcggggtg gaggtgctga
     7261 tccccgactt ccgcggcagc ccggaggcgc tgcggcaagt ggtcgacgcg actcccgacg
     7321 tgctcggcca caacgttgag acggtcccgc gcctctacaa gcggatccgg ccggggttca
     7381 cctacgccgg cagcctggag ctgctggcca gggcgcgggc gtggctgccg cagggctcgg
     7441 cgaccaagtc caacctgatc ctgggcatgg gcgaggagcg cgacgaggtc aaccagacca
     7501 tgcgccacct gcgcgaggtg ggcgttgagc tgttgaccat cacccaatac ctgcagccaa
     7561 gccgccagca cctgaacctg cagcgcttcg tcaccccgga ggagttcgcc gagctgaagg
     7621 ccgaggccga ggggctcggg ttcgcccacg tggagtccgg gccgctggtg cgctccagct
     7681 accacgccgg gcgcatgcac gaggcggcga cgggagcggc tcgggcggcg acgcggtgac
     7741 ggcgtccgcc ctccaccttg gacgtcctgg acgccgggcc agggtcacgg tggcggcggt
     7801 gctgctggtc gccctggtcg gctggctcgg gtggcgtgcc cagcaggcgt ttgaccatag
     7861 ccgccatctg gccgccgccc gtgcggtggt cagcgctccg gacgtggtca cctaccggct
     7921 ccccgcgagc ggggtcggga gcagggtcag cgggatgctg cagtaccggc gcggggagtg
     7981 gctgtcggtg gtgaccctgc acggcctgtc accggtcggc gggcgcgagc actacctggt
     8041 gttcctgcac ggcttcagtg gctggacgct tgccggggcg gtccgcccca acccggacgg
     8101 ctccgcgcag gtgcgcttcg gcgccgagcc acgcccctcc tccacctatg aggtggtcgt
     8161 cacccgaggg gtcgacgacg ccaccagcat cccccacggc accatgctgc tgcactggtt
     8221 cgacgtcggc ctcggccccc tccgcgtgaa gccgttcgac ccggcgcacc cggtccccta
     8281 gcctctgccc taggggcttc tcgctgcctg tccggattcc gctcggcagt tcttggtatc
     8341 tcgttaggga ttcgtgagcg gcgcgcggtt tcctctgctt gtcgggtgga acagatcggg
     8401 cccggtcgcc acccccctga ccgggcccga tccactcgca ggaccggggg ctggggtacg
     8461 caggttgcag gtgaggaggc agcgcatgac caggaccgct ctggaccttc gccgaccgcg
     8521 ggtcgcgctg gtcgcctcac cgcgggccac ccttccgccc ggcagctatg gcggcagcgt
     8581 cgagcaggtc accgggctgg cagcggcgct ggcgcggcgc ggctatgaga tcacggtcat
     8641 cggcgctggg ctcacgatag cgccaggcgc ttcgcgctac cgggcgatcg acacccagca
     8701 ggacgagggc ctgaccgacc cggtgctcgc cgaggcgctg cacggcgtcg cggtcgagcg
     8761 cctgctccgc cgcctggacg tcgacctggt ccacgaccac accgtcatcg gcatgctcac
     8821 ccccgccagc gaccgcgaca tcccgcggat cgtgaccgtc tacaccccca ccagtgacgc
     8881 cgacggcgta ccgagcgcca aggtcggcgg cagggtcggc ggcggcgccg cgctggtcgc
     8941 ggtatccggg caccagcgcg cccaggcgcc ggggctcccc tggctcggtg tcgtccaccc
     9001 cggcatcctc gtcaccgaat acccctaccg ggaggagaag gacgggtcct gcctgttcgt
     9061 cggggcgctt gcccccgggc gcggggccga cctggcgatc gccgccgcgc acgcggcggg
     9121 gcggtcgatc acggtcgcgg ggaccctgcc gcagccgggg atggcgacct acgtgcagtg
     9181 gcggctgcgg ccgctgctgc gccccggcga caagctgatc gtcgcggccg accatgccaa
     9241 gcgcaccagc ctgctgctgc acgcgcgctg cctggtcgcg ccgttcggcg ctgacgttcc
     9301 cttctcgctg ccggtggtcg aggcgctggc ctgcggcacg ccggtggtgg cgctgcgcgg
     9361 cggcgtcgcc gaggagctgg tcgagcacgg cgtctccggg atcctctgcc caaccccggc
     9421 ggagctggcc gaggggatcg ccgccgctga gcggctcgac ccggccgact gccgccgcca
     9481 cgccgccgcc cgcttcgaca ccacccagat ggccgccggc tacgaccggc tctactccca
     9541 ggcgctggcc gcacggctcc agcagacacc cgccgcgtcg cccgatcccc acccccaccg
     9601 ggcgcccgtc cccgccggcg gacggtcctg gccctgatct tggccgcgcc tcccagggcg
     9661 ggccggtgct ccgatcaccg gcccgcccgc acgttctgtc ctagttaccg cgatctaccg
     9721 catcggaata tttcagccgc tccgagagca gcgttaggat tcccccttca ctgaccgaat
     9781 agggggaatc atgcgccgct caaggttcct gctcctgctc accatcagtg ccgcgctgct
     9841 gccgctggcg ctgcccgccg ccgggctggc acccgctctg gcgcgggaga ccccggccac
     9901 gggcgccaac gccgccgcct tcaccgcgac cgccaccggc caggtcttcg acccgaaccc
     9961 ggtcgtcacc ctgcaggacg agaccctcac cgaccagcag gacaaggact acccggcgct
    10021 gaatcccgcc taccggacgg tgacgctgac ccgcctggac gggtcggggt acctccacgg
    10081 cgacttcgcc accatcgacc ccaagcagaa gacaccggcg ttctcctcca ccgggcagtt
    10141 cgtctacctt cgctccgacg accgcttcga gcaggtgatg gcctactacc acgtcaccgc
    10201 cgcgcagaca tacatccaga gcctcggctt caccaccatc aacaacgagc cgcagaacct
    10261 gctgctcaac ccgatcgggc aggacaactc ctactacgac ccgaagaccg acatcatcac
    10321 cttcggcaaa ggcggcgtgg acgacgccga ggacgccgag atgatctggc acgagtacgg
    10381 ccacgccatc caggacagcc aggtccccgg gttcggctcc ggcaaccagg ccggttcgat
    10441 cggcgagggt ttcggcgact actgggcctt caccatgtcc caggccgaca gccccgacac
    10501 cgccaccacc ccgctggcct gcatcgccga ctgggactcg accgcctata cctacaacca
    10561 actgccgcac tgcctgcgcc gggtcgacac caacaaaacc atggccgact accagccgaa
    10621 cggcgacgtg cacgtcaacg ggcagatctg gtcgcgggcg ctgcacgaca tcaacctcgc
    10681 actcgggcgc gacccggcca acaccgtgat cctccaggcg cagttcgcct tcagcccctc
    10741 gaccaccttc caggccgccg cgcaggccac cgtggacacc gccaccgccc tgtacggctc
    10801 ggccaccgcc agccaggtcc aggcggcgtt cgcggcgcgc gggatcctct gagagcgctg
    10861 gggtacgatc gacggcgcgg cgagacctgc gcgttaacgg acgcgaaaca aggcagcaac
    10921 caggcagcaa cggccacttg ctagcgtcgg acaggcctgg gccagcgccg caggatggat
    10981 ctgcgcggtt gtggagcttc agcggttgat tggctgttcg agttttcggg aaatgggggc
    11041 gccccccctg atgcgcaaag gaggcagcgc gtgacggatt cggtcggtga cgtcctcaag
    11101 ctgatcaagg accgcggggt ccagatcatc gacctgcgct tcgcggacct gcccgggctg
    11161 acgcagcact tctcggtgcc gccctcggag ctgaccgagg cgtcgttcga ggaaggcttc
    11221 ggcttcgacg gctcctcgat ccgcgggttc caggagatcc aggaatcgga catgctcctg
    11281 ttcccggacc cgggcaccgc ggtgctcgac ccgttcgcca agcacaccac cctggtgctc
    11341 aactgcttcg tcggcgaccc agtgaccggc gagccctaca gccgcgaccc gcgccacatc
    11401 gcccgcaagg ccgaggcgta cctcaagggg accggcatcg ccgagacctc gtactggggc
    11461 ccggagtgcg agttttacat cttcgactcg gtccgcttcg accagaacca gtactccgcc
    11521 taccacttca tcgacgccat cgagggccag tggaactccg gcgccgagga gggcggccac
    11581 aacctcggct acaagccgcg ctacaaggaa ggctacttcc cggtcccgcc gaccgaccac
    11641 taccaggaca tccgctccga gatggtcctg aagatgatcg aggcaggcat cggcatcgag
    11701 gtgcaccacc acgaggtcgg caccgccggc caggccgaga tcgacatgcg cttcgacacc
    11761 ctgctgcgca tggccgacaa ggtgatgctc tacaagtaca tcgtcaagaa cgtcgcccgc
    11821 gcctggggca agaccgccac cttcatgccc aagccgatct tcgaggacaa cggctcgggc
    11881 atgcacaccc accagagcct atggaccaac ggcgagccgc tgttctacga cgagtccggc
    11941 tacgcgggcc tgtcggacac cggtcgctgg tacatcggcg ggctgctcgc ccacgccccg
    12001 gcgctgaccg cgctgtccaa cccgaccacc aacagctacc gccgcctggt ccccggctac
    12061 gaggcgccgg tcaacctggt ctactcgcag cgcaaccgct cggccgcctg ccgcatcccg
    12121 ctctactcca agagccccaa ggccaag
//
LOCUS       DASWDC010000011        16897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_585286, whole genome shotgun sequence.
ACCESSION   DASWDC010000011 DASWDC010000000
VERSION     DASWDC010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16897
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585286"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VF995_00290"
     CDS             <1..581
                     /locus_tag="VF995_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9342039.1"
                     /translation="IAYGGRRVDGPRRELGYLTQSDTLMPWRDVRRNVELPLELRGVD
                     KRERRAVAHRLIDTVGLHGFERHYPRELSGGMRRRASLARMLCGDPETLLLDEPFGAL
                     DAQLRAELQGELLRLWSGSGRTVVFVTHDLEEALLLGDRVVVLGHAGRLVLDQPVDLP
                     RTRDLDEIRLHPRFVELHRVVSTALKDASRSP"
     gene            578..1501
                     /locus_tag="VF995_00295"
     CDS             578..1501
                     /locus_tag="VF995_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9342040.1"
                     /translation="MTTPPRQLPAAAPTPAPVSAGGGTGTDGAVSLGTRAGHGPIELP
                     AERSWWQRHGETAIVWAFRVLLLAAVLVAWQLASGRLVDELFASKPSAVAHRLAQWSG
                     DGTLWSNTWLTIQEILVGYLLGASTGVLAGFALASQRIAWRVLEPFVMALYSIPKVAL
                     APLFIVWLGIGLQMKVLVAAVSVFFLVFINTAAGIRGVDRGLVDAVRLMGGSRRDIAL
                     KVILPGAMAGVLTGLRVAIPYALIGAVIAELVASNRGLGYLINDAAARFDTAGVFAAL
                     VVLSVIASVLGALVSVLGRFTNRWKPIDERH"
     gene            1572..2624
                     /locus_tag="VF995_00300"
     CDS             1572..2624
                     /locus_tag="VF995_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9342041.1"
                     /translation="MRDRTTAGRMARFLAACATVGLLAAGCGGGKSSSSGDMTLNVGQ
                     VSNSVAFLPFYIAEQQGYFTQEGVKLGDRPRLGTGAKVAAALTSGSIDIAGSVMTDVL
                     NLYKTRSDTRLLAPLVNAYYIDVTVRTGFTDPGGDLNAKITALKGKKIGITGPGSGTE
                     ALLIYLFKQVGLDTKRDATLVPLGSDATAALGALKTGRVDALSLAQPVGQQAEADGIG
                     KIYISPTNGDVPALVGATQGVVFTTQKVIDRKGAAVAAFLRAIAKAEQLIHDDPATAQ
                     KLLAGYQNTLKPATVQTLMPIVEKEIPAKPVFTEQGYQTIVTFHTSSGLAASAPPFAT
                     VVPTAWINQALAGSKG"
     gene            complement(2627..3526)
                     /locus_tag="VF995_00305"
     CDS             complement(2627..3526)
                     /locus_tag="VF995_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX9342042.1"
                     /translation="MKPADFCLHRPDILGEALALLAEHGDDAKALAGGQSLVPLLNFR
                     LARPAHVIDLERIASLRRLRRSADGLAVGAMVRQRHAETDPEVAEACPLLAAALPHVG
                     HPPIRNRGTIGGSLAHADPAAELPAVAVALDATLIAASARSRREIPASAFYQTYLTTA
                     LEPDELLVETRFPPLPPRSGACVLEVSRRQGDFALVGVALQVTLDDGGTVADARVCFI
                     GVAEVPRRCPEAEAVLTGCSPADAEAVSAAADRAREVLTPASDLHASADYRRDVAGTL
                     LERAFIGAARRPSRRAAPDPRPR"
     gene            complement(3536..4612)
                     /locus_tag="VF995_00310"
     CDS             complement(3536..4612)
                     /locus_tag="VF995_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9342043.1"
                     /translation="MTGPPSPVPVPGSVTVVDAHAHAVPADLVADLVGGRVHFPNVSV
                     GPGPGPAEAAGPGPTFAFAGGPPTRPLAPALTDAGRRRAWLQAQGIGAQVVGGWLDIF
                     GYQLGSAEGADWSRLLTERLAGLCAREGEGRLTPLGTVPLQDPARAADLLPEVLAAGC
                     AGVMVATRAGELELDDPAATPFWEAADDLGTVVYLHPGFGAASRRYEPYGLANGLARI
                     EDSTVTLARLLYAGIPARYPRLRLVVAHGGGALPWVLGRLVRNYEVSGARHADPMESF
                     ARLYFDSVVFEPAALELLVRLAGPERVLLGSDYPFPIGDPQPRQVIERSGLSEADARL
                     LLGGNAATVFGLPLPAGGDGAAAP"
     gene            complement(4609..7035)
                     /locus_tag="VF995_00315"
     CDS             complement(4609..7035)
                     /locus_tag="VF995_00315"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9342044.1"
                     /translation="MAEPGPGPEPGWRFVSRPARVREDRRFVTGHGRFVADLTPPGTL
                     HVALVASVHAHARIAGIDPGAALALDGVVAVVTGAQLAEDTEPMRQYVDAPGVAWRPL
                     ALETARYAGEWVAAVVATSRAVAEDGAELVEVDYQPLPVILDPERAQDPGAPLVHPAH
                     GSNVVVHREFVWGDVDGDFATADGTARLRVRWQRSATVPLETFGVVASWDPDGGLLEV
                     WASVQMPQFPDQLAHCLRLATNRVRVHFDVDVGGSYGTKRGIKHGVLAGYLARLLGAP
                     VKLVEDRAEHMHGGDFHGPDRRFDVELAYQRDGTITALRLAALDDQGAYPSRSVLQLA
                     KPVGSIVGPYRIRSVAYRATGVLTNKPGQVAVRGFGQAPTNYAIESAVDAVAAALGMD
                     RLELRRRNLIRAEQFPYRIPSGTTYDSGDYHTVLDQLEAAGLAALTERRDQLRAQGLL
                     AGIGVATCLEPSGGNATFEQLLHPGNDKTTFPEGCRLRVDADGQVIAVIALSSAGQAH
                     ESLVATLVGEELGRDPADVMVVRADSLSGLPSQSPVGSRMAIMLGAGVRGAADALKRK
                     LLAVAAHNLGVGTDGLAYDRGDVLVRADPARRLSLQELALIAHRHAHRMPPGMEPGLE
                     ALHVAQVPGGGTLAAVDGSVAMYPCFAFEAHLALVSIDPGTGQVAIPEYYVAHDCGTM
                     INPQVVRGMVLGGIAHGIGVALYERFAFDEHGQPLTRSFMDYLLPSIYEVPHLRDLEH
                     HTPSPLTSHGQKGSGEAGYMGAPAAVASAVNDALAAGGAAPVQALPIGPADVWAALRA
                     ARRPASGGPQ"
     gene            complement(7028..7540)
                     /locus_tag="VF995_00320"
     CDS             complement(7028..7540)
                     /locus_tag="VF995_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9342045.1"
                     /translation="MVVNGTPRAVRAAARTSLADLLREQLDLTGTRLGCEHGVCGCCT
                     VLLDGEPVRSCIVLAAQADGHRVTTVEGLAGPDGSLSVVQEAFCDTHALQCGYCTPGM
                     LLAAHALLERTHTPSSGEIEEALGGNLCRCTGYVQIREAVALAARRLAASDAQASDAP
                     ASDGQAGRHG"
     gene            complement(7612..8469)
                     /locus_tag="VF995_00325"
     CDS             complement(7612..8469)
                     /locus_tag="VF995_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9342046.1"
                     /translation="MIRNQDFQHSGLTLRSTVHIPDSPPGTRWPTVILIHGFSANRLE
                     FGSSFVHMSRLLERNGIASVRVDLSGHGESDGDFFDVTITGEIAETRSVVQVVRAFDF
                     ADPARIGLLGMSMGGVVAGIVAAEEPGIRALCLWSPAAVAPFEIGSGHLKGRNIAAQL
                     EAKGYFDADGHRVSPALVDDITDLDVYGRARHYTAPSASSTATRTTSPPSPTPAATST
                     TTATRNSRSSLTPTTAGPPSRTAPACISRLWSSSASTFSNEGIEVERLQPTPASAQWG
                     DGAQNDGFR"
     gene            complement(8466..10991)
                     /locus_tag="VF995_00330"
     CDS             complement(8466..10991)
                     /locus_tag="VF995_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX9342047.1"
                     /translation="MTLEPEPELKAKLAALTLEQKVRLLTGAGFWSLHPEPAIGLRRV
                     VVSDGPAGVRGERWDERDPSANVPSPSALAATWDEARVERLGRLLAAEARRKGVQVLL
                     APTVNLHRSPYGGRHFECFSEDPLLTARIGAAYVRGVQSGGIAATVKHFVANDSETER
                     HTVDVRVDERTLRELYLAPFEAIVREAGAWAVMAAYNSVNGTTMSEHPLLREVLKDEW
                     GFDGVVMSDWFGTRSTVPSGRSGLDLVMPGPSGPWGDDLVAAVSDGHVSEAAIDDKVL
                     RLLRLAARVGALDLDLGADRLEAEREPWSSAAVAVELRATAAAGFVLVRNEHARDHDG
                     ESERAVLPLEAGRLRRVAVLGPNAAVPRTLGGGSATVFPPYAISPLDGLRVALEPEVE
                     VAHAPGARATTGIPPADLGLLRPPASSPEGEEGGVELRFVAADGTVLHREHRRTAAFT
                     WLGSFGDFPAERVAAIEVRTRLLADASGDWTVGCSGVGDFELSLAGTPRFAGPLAPEP
                     GVEPIGAMMRPPQHLVRVPLEAGQQLPITLSYRLTDASRIPAFQLNVDRPCGSDEEEL
                     ARAVALARDADVAVVVVGTTDEVESEGFDRNSLALPGRQDDLVRAVAAANPHTVVVVN
                     AGAPVLLPWADEVPAILLTWFPGQEFGNALADVLLGANEPGGRLPTTWPASPDGLPTT
                     TPAAGRLDYAEGIFVGYRAPEGRAVQYPFGHGLGYTTWEYLDVKTTGHATLTPGGDLG
                     PGSTLTVHVTVRNAGSRPGCEVVQVYASRPDSTLPRPPGWLAGFALAHAIPGETATVE
                     VLVPARTLAHWDAEAKAWRIEPGSWALTAGPVATAVPAGEAAS"
     gene            complement(10988..12472)
                     /locus_tag="VF995_00335"
     CDS             complement(10988..12472)
                     /locus_tag="VF995_00335"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HEX9342048.1"
                     /translation="MTANPAERPVNLAAIDLGASSGRVLLGRIGGGDFEVTEVHRFPN
                     IPVRAQETLHWDVLALHQGILDGLRAAARQAGRLDGVGIDSWGLDYGLLDATGALLGN
                     PVHYRDARTDGILDKVLREVPAADIYAVTGQQFLPFNTLCQLVAARGTPQLEAAARLL
                     QIPDLFAYWLTGVEGAEITNASTTQLLDVTARTWAADLIRRLGLAPGLFPPLREPGDP
                     ASELLPSVLEAVGLAGQGPVPVTAVGSHDTASAVAGVPADGERFAYISCGTWALVGVE
                     LDQPVLSEESRQANFTNELGVDGTIRYLRNVMGLWLLQESLRVWSDADGGRPVDLEAL
                     LREAAGLPAFCAVIDVDDPVFVPPGDLPARIAATCEYTGQTAPQSPAETVRCVVDSLA
                     LAFRRTLHDAQRLSGRDVEVIHMVGGGARNELLCQLTADVCELSVVAGPAEATALGNL
                     LIQARAQRAAPADLPGLRSLLRQTQSLRTYLPRRRGRPTPEVPG"
     gene            complement(12469..14547)
                     /locus_tag="VF995_00340"
     CDS             complement(12469..14547)
                     /locus_tag="VF995_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HEX9342049.1"
                     /translation="MAHPEVTALLGRSNRLGSDPANTNYAGGNASVKAAATDPVTGEP
                     VELLWVKGSGGDLGTLTESGLAVLRLDRLRALTGVYPGVEREDEMVAAFDYCLHGKGS
                     AAPSIDTAMHGLVEAAHVDHLHPDAGIALATAVDGEALTKACFGERVAWVPWRRPGFQ
                     LGLDVAAVKAAHPQAIGVILGGHGITAWGETSDACQANSLEIIRSAQRFIDEHGKADP
                     FGAVVAGFEPLPPAERHARAAALLPLLRQLASTDAPTSRPRVGHYTDSDAVLDFLARE
                     KLQPLAALGTSCPDHFLRTKLRPLVLDLPASAPLEAVAARLRELHAVYREDYRAYYER
                     YAEQEPGPIPPLRGADPAIVLVPGVGMCSFGVDKQTARIAGEFYLNAINAMRGAEALS
                     SYAPIGEREKFRIEYWALEEAKLQRLPKPKPLAGRVAFVTGAGSGIGRAIASRLAAEG
                     ACVAVADRDAASACAVAEEIAADGHVLDPADIAIAMTVDVTDADAVAAALRQTVLAFG
                     GVDLVVNNAGLSISKPLLETTELDWDLQHDVMAKGSFLVSREAAHIMVEQGLGGDLVY
                     ICSKNALFAGPDNVAYGAAKADQAHQVRLLAAELGPHGIRVNGVNPDGVVRGSGIFAG
                     GWGAQRAAVYGVPQAELGAFYAKRTLLGREVLPEHVANAVFVLTGPELSHTTGLLVPV
                     DAGVAAAFLR"
     gene            complement(14559..15725)
                     /gene="rhaI"
                     /locus_tag="VF995_00345"
     CDS             complement(14559..15725)
                     /gene="rhaI"
                     /locus_tag="VF995_00345"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEX9342050.1"
                     /translation="MRDLVAVKAALRQQRIELPSWAFGNTGTRFKVFTQPGVPRDAYE
                     KVADAATVHRFTGVAPSVALHIPWDAVDDYAHLAKHADDLGVQVGTINANVFQDDDYK
                     LGSVCNPDPRVRRKALDHLLACVEVMDATGSRDLKLWFADGTNYPGQDSIRARQERLA
                     EALAAVYRRLGPEQRLLLEYKLFEPAFYATDVPDWGTALLHCQALGDRAKVVVDTGHH
                     APGTNIEMIVALLLRAGKLGGFDFNSRFYADDDLMVGAADPFQLFRIMHEVVRGGGYA
                     PQGEIAFMLDQCHNIEPKIPAQIRSVMNVQEATAKALLVSAAALAGMQLAGDVLGANA
                     VLMDAYATDVRPLLAELREEQGLDPDPVAAYHRSGYGERIVAERVGGTQAGWDA"
     gene            complement(15785..16696)
                     /gene="rhaS"
                     /locus_tag="VF995_00350"
     CDS             complement(15785..16696)
                     /gene="rhaS"
                     /locus_tag="VF995_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9342051.1"
                     /translation="MTSADINCSTAYPYFTTSDNGGKTAVGEFAGTYKEVGPSDASAS
                     AQVPYINTLTQQHQDAIVISGDDPNAVVPALKAAMAAGIKVVTYDADTAAAGRTVFAN
                     QASPEGIGRSEVQVLGKQLGYSGQIAILSATSTAANQNTWIKFMKNELTKPQYAKMKL
                     VKVAYGNDDDQTSFQQAQGLLQAFPNLKGIISPTTVGVAAAARYLSGSSFKGKVALTG
                     LGTPNQMRKFVKDGTVQAFELWDPGKLGYLAAYAAAALCSGQITGKPGETFKAGNLGT
                     KTIGANGEILLGPPQVFDKSNIDQFNF"
BASE COUNT         2322 a         5885 c         6365 g         2325 t
ORIGIN      
        1 cgatcgccta cggcggccgc cgcgtcgacg ggccccggcg ggagctcggc tacctcaccc
       61 agagcgacac cctgatgccc tggcgggacg tgcgccgcaa cgtggagctg ccgctggagc
      121 tgcgcggggt cgacaagcgg gagcggcgtg cggtggcgca ccgcctgatc gacactgtcg
      181 ggctgcacgg cttcgagcgg cactacccac gggagctgtc cgggggcatg cgccgccgtg
      241 ccagcctcgc ccgcatgctc tgcggtgatc cggagaccct cctgctcgac gagccgttcg
      301 gggcgctcga cgcccagttg cgcgccgagc tgcaaggcga gctgctccgg ctgtggtccg
      361 ggtcgggccg cacggtggtg ttcgtcaccc acgacctcga ggaggcgctg ctgctgggcg
      421 accgggtggt ggtgctcggc cacgccggcc gcctggtgct cgaccagccc gtcgacctgc
      481 cgcgcacgcg cgacctcgac gagatccggt tgcacccgcg ctttgtggaa ctgcaccggg
      541 ttgtgtccac ggcgctcaaa gacgcttcga ggtcgccatg acgaccccgc cacgccagct
      601 cccggctgcg gccccgaccc ccgctcccgt cagtgccggc ggcggcaccg gcactgacgg
      661 ggcggtcagc ctcgggacca gggcgggcca cggcccgatc gagttgccgg ccgagcggag
      721 ctggtggcag cggcacggag agaccgccat cgtgtgggcc ttccgggtac tgctgctcgc
      781 cgccgtcctg gtcgcctggc agctggcctc cggccgcctg gttgacgagc tgttcgccag
      841 caagccgtca gccgtggcgc accggctcgc ccagtggtca ggcgacggca ccctgtggtc
      901 gaacacctgg ctgactatcc aggagatcct cgtcggctac ctcctcggcg cctccaccgg
      961 cgtgctcgct gggttcgcgc tggcatcgca gcgcatcgcc tggcgggtcc tggagccatt
     1021 cgtgatggcg ttgtattcga tcccgaaggt ggcgctggcg ccgctgttca tcgtctggct
     1081 tggcatcggc ctgcagatga aggtgctcgt ggccgccgtc agcgtcttct tcctggtgtt
     1141 catcaatacc gccgcgggca tccgcggcgt cgatcgcggc ctggtcgatg ccgtgcggct
     1201 gatgggcggc agccgccgcg acatcgccct caaggtgatc ctgcccggcg cgatggccgg
     1261 tgtgctcacc ggcctgcggg tcgcgatccc ctatgcgctc atcggcgccg tcatcgccga
     1321 gctggtcgcc tccaaccgtg ggctcggcta cctcatcaac gacgccgcgg ccaggttcga
     1381 caccgccggg gtgttcgccg cgctggtggt cctgagcgtg atcgcgtccg tgctgggcgc
     1441 gctggtcagc gtgctcggcc gcttcaccaa ccgctggaag ccgatcgacg agcgccactg
     1501 acggccgcgc cggtcccacc ccacccccca cgcccacccg gtgaagggaa cccccgatga
     1561 agggagccga catgcgagac cgcaccactg ccggccggat ggcccgattc ctcgccgcat
     1621 gcgcgactgt gggattgctc gccgccggct gcggcggagg caagagctcc tccagcggcg
     1681 acatgaccct caacgtgggg caggtcagca actcggtcgc gttcttgccc ttctacatcg
     1741 ccgagcagca aggctacttc acccaggagg gcgtcaagct cggcgatcgg ccgcgcctgg
     1801 ggaccggcgc caaggttgcc gcggcgctca cctccggctc catcgacatc gccggcagcg
     1861 tcatgaccga cgtgctcaac ctgtacaaga cccgcagcga cacccgcctg ctggcgccgc
     1921 tggtcaacgc ctactacatc gacgtcaccg tgcgcactgg cttcaccgac cccggcggcg
     1981 acctcaacgc caagatcacc gcgctgaagg gcaagaagat cggcatcacc ggtccgggca
     2041 gcggcaccga ggccctgctc atctacctct tcaagcaggt ggggctggac accaagcggg
     2101 acgccactct ggtccccctc ggcagcgacg ccactgcggc gctcggggca ctcaagaccg
     2161 gccgcgtcga cgcgctctcc ctggcccagc cggtcggcca gcaggccgaa gccgacggca
     2221 tcggcaagat ctacatctcc cccaccaacg gggacgtccc cgccctcgtt ggcgccaccc
     2281 agggcgtggt cttcaccacc cagaaggtga ttgaccgcaa gggcgcggcg gtcgcggcgt
     2341 tcctgcgcgc gatcgccaag gccgagcagc tcatccacga cgaccccgcc accgcgcaga
     2401 agctgctcgc cggctaccag aacaccctca agcccgccac ggtccagacc ctcatgccga
     2461 tcgtggagaa ggagatcccc gccaagccgg tcttcaccga gcagggctac cagaccatcg
     2521 tgaccttcca cacctccagc ggcctggcgg cctccgcccc tccattcgca accgtcgtcc
     2581 cgacggcctg gatcaaccag gccctcgcgg gcagcaaggg gtagggctac ctcggcctcg
     2641 gatccggggc ggcccgccgt gacgggcggc gggccgcccc gatgaaggca cgctccagca
     2701 gggtgccggc gacgtcgcgg cggtagtcgg cgctggcgtg caggtcgctt gcgggggtga
     2761 gcacctcgcg ggcgcggtcg gcggcggcgc tgacggcctc ggcgtcggcg ggcgagcagc
     2821 cggtgagcac agcctcggcc tccgggcagc ggcgcggcac ctcggcgacg ccgatgaagc
     2881 acacccgggc gtcggcgacg gtgccgccgt cgtccaaagt gacctgcagc gcgacgccga
     2941 ccagcgcgaa gtcgccctgc cggcggctca cctccagcac gcacgcgccg ctgcgtggcg
     3001 gcagcggcgg gaagcgggtc tctaccagca gctcgtcggg ctccagggcg gtggtcaggt
     3061 aggtctggta gaaggcggac gccgggatct cccggcggct gcgggcgctg gcggcgatca
     3121 gcgtcgcgtc cagtgccacc gccaccgctg gcagctcggc ggccgggtcg gcgtgggcga
     3181 ggctgccgcc gatcgtgccg cggttgcgga tgggcgggtg gccgacgtgt ggcagggcag
     3241 ccgcgagcag cgggcaggcc tcggcaacct ccgggtcggt ctcggcgtgg cgctgccgga
     3301 ccatcgcgcc caccgcgagg ccgtccgcgc tgcgccgcag ccgcctgagg gaagcgatgc
     3361 gctccaggtc gatgacgtgg gcgggccggg cgaggcggaa gttgagcagc gggacgaggc
     3421 tctgcccgcc agcgagggct ttggcgtcgt cgccgtgctc ggccagcagc gcgagcgcct
     3481 cgccgaggat gtccgggcgg tgcaggcaga agtcggccgg cttcatgacc tcacctcacg
     3541 gcgccgcggc gccgtcgccg ccggccggga gcgggaggcc gaacacggtg gcggcgttgc
     3601 cgccgagcag caggcgggcg tcagcctcgc tcaggccgga gcgctcgatc acctggcggg
     3661 gctgcgggtc gccgatgggg aacgggtagt cggagccgag cagcacccgc tcgggaccgg
     3721 ccagccgcac cagcagctcc agggccgccg gctcgaagac gaccgagtcg aagtacaggc
     3781 gcgcgaagga ctccatcggg tcggcgtggc gagcgccgct cacctcgtag ttgcggacca
     3841 gccggccgag cacccatggg agcgccccgc cgccgtgggc gacgaccagc cgcagcctcg
     3901 ggtagcgggc gggaatgccg gcgtacagca ggcgcgcgag ggtgacggtg ctgtcctcga
     3961 tgcgggccag cccgttggcc aggccgtagg gctcgtagcg gcggctcgcc gcgccgaagc
     4021 ccgggtgcag gtagacgaca gtgccgaggt cgtcggccgc ctcccagaag ggcgtggcgg
     4081 cggggtcgtc cagctccagc tccccagccc gcgtggcgac catcaccccg gcgcagccag
     4141 cggccagcac ctcggggagc aggtcggcgg cgcgtgcggg gtcctgcagc ggcacggtgc
     4201 cgagcggcgt cagccgcccc tccccctccc tggcgcacag cccggccagg cgctcggtga
     4261 gcagccgcga ccagtccgcc ccctcggcgc tgccgagctg gtagccgaag atgtccagcc
     4321 agccgccgac gacctgtgcc ccgatgccct gcgcctgcaa ccaggcacgg cggcggccgg
     4381 cgtcggtgag cgcgggcgcg agcggccggg tcggcggacc gccggcgaag gcgaaggtgg
     4441 gaccggggcc agcggcctcg gcggggccgg ggccggggcc gacgctgaca ttggggaagt
     4501 gcacgcggcc accgaccagg tcggccacga gatccgcggg caccgcatgc gcgtgcgcgt
     4561 ccaccaccgt caccgaaccc ggcaccggca ccggcgatgg cgggccggtc actgcggacc
     4621 cccgctggcg gggcgccggg cggcgcgcag tgcggcccaa acgtcggccg ggccgatcgg
     4681 cagggcctgg accggggccg cgccaccggc tgcgagcgcg tcgttgaccg cgctcgccac
     4741 ggcggcgggg gcgcccatgt agccagcttc gccggacccc ttctggccgt gggaggtcag
     4801 cggcgagggc gtgtggtgtt ccaggtcgcg gaggtgcggc acctcgtaga tgctgggcag
     4861 caggtagtcc atgaacgacc tggtgagcgg ctggccgtgc tcgtcgaagg cgaagcgctc
     4921 gtagagggcg acgccgatgc cgtgggcgat gccgcccaag accatgccgc ggaccacctg
     4981 cgggttgatc atcgtgccgc agtcgtgggc cacgtagtac tcggggatgg cgacctgccc
     5041 ggtgccaggg tcgatgctga ccagcgccag gtgtgcctcg aaggcgaagc acgggtacat
     5101 cgccacgctg ccgtcgacgg cggcgagcgt gccgccgccc ggcacctggg cgacgtgcag
     5161 cgcctccagg ccaggctcca tgccgggcgg catgcggtgg gcgtggcggt gggcgatcag
     5221 cgccagctcc tggaggctga ggcggcgggc gggatcggcc cgcaccagta cgtcgccacg
     5281 gtcgtaggcg agcccgtcgg tgccgacgcc gaggttgtgg gcggcgaccg cgagcagctt
     5341 gcgcttgagg gcgtcggcgg caccgcgcac gccggcaccg agcatgatcg ccatgcggct
     5401 gcccacgggg ctctgcgacg gcagcccgga cagggagtcg gcgcggacca ccatgacgtc
     5461 agcggggtcg cggcccaact cctcgccgac cagggtggcg accaggctct cgtgggcctg
     5521 ccccgccgag gacagggcga tgaccgcgat cacctgcccg tcggcatcga cccgcaggcg
     5581 gcacccctcc gggaaggtgg tcttgtcgtt gccgggatgc agcagctgct cgaaggtagc
     5641 gttgccgccg ctcggctcca ggcaggtggc gacgccgatg ccggcgagca ggccctgggc
     5701 gcggagctgg tcgcgccgct cggtgagcgc ggccaggccg gccgcctcga gctggtcgag
     5761 gacggtgtgg tagtcgccgg agtcgtaggt ggtgccgctg gggatgcggt aggggaactg
     5821 ctcggcgcgg atgaggttgc gccgtcgcag ctccaggcgg tccatgccga gtgcggcggc
     5881 gaccgcgtcg acggcggact cgatggcgta gttggtgggc gcctggccga agccgcgcac
     5941 tgccacctgc ccgggcttgt tggtgagcac gccggtggcc cggtaggcga cgctgcggat
     6001 gcggtagggc ccgacgatcg agccgaccgg cttggcgagc tgcagcaccg agcggctcgg
     6061 gtaggcgccc tggtcgtcga gggccgccag ccgcagcgcc gtgatggtgc cgtcgcgctg
     6121 gtaggccagc tcgacgtcga aacgacggtc ggggccgtgg aagtccccgc cgtgcatgtg
     6181 ctcggcgcgg tcctcgacca gcttgacggg ggcgccgagc agccgcgcga ggtagccggc
     6241 gagcacgccg tgcttgatgc cgcgcttggt gccgtagctg ccgccgacgt cgacgtcgaa
     6301 gtgcacccgc acccggttgg tggccaggcg caggcagtgc gccagctggt cggggaactg
     6361 gggcatctgc accgacgccc acacctccag cagcccaccg tcagggtccc agctcgcgac
     6421 caccccgaag gtctccagcg gcacggtggc gctgcgctgc cagcgcaccc gcagccgcgc
     6481 ggtcccgtcg gccgtggcga agtcgccatc gacgtcgccc cagacgaact cgcggtggac
     6541 caccacgttg ctgccatgcg ccgggtggac cagcggcgcg ccagggtcct gggcgcgctc
     6601 cgggtcgagg atcaccggca acggctggta gtcgacctcg accaactcgg cgccgtcctc
     6661 ggcgaccgcg cgggaggtgg ccaccaccgc cgccacccac tcgccggcgt agcgggcggt
     6721 ctccagcgcc agcggccgcc acgccacgcc gggggcgtcc acgtactggc gcatcggctc
     6781 ggtgtcctcg gcgagctgcg cgccggtgac caccgcgacg acgccgtcca gcgccagcgc
     6841 ggcgcccggg tcgatcccgg cgatacgtgc gtgagcgtgc acactcgcga ccagcgcgac
     6901 gtgcagcgtc cccggcggtg tgaggtcggc gacaaaccgc ccgtgcccgg tgacgaaacg
     6961 gcggtcctcc cgcacccgcg ccggccgcga cacgaaacgc cagccgggct caggtcccgg
     7021 ccccggctca gccatgccga cccgcctgcc cgtcgctcgc tggcgcatcg ctggcttgcg
     7081 cgtcgcttgc ggcgaggcgg cgagccgcca gcgcgacggc ctcgcggatc tgcacgtagc
     7141 cggtgcagcg gcagaggttg ccgccgagcg cctcctcgat ctcgccgctg ctcggagtgt
     7201 gggtgcgctc cagcagcgcg tgcgcggcca gcagcatgcc cggtgtgcag tagccgcact
     7261 gcagcgcatg ggtgtcgcag aacgcctcct gtaccacgct gaggctcccg tccggcccgg
     7321 ccagcccctc cacggtggtg accctgtgcc cgtcagcctg tgcggcgagc acgatgcagg
     7381 aacggaccgg ctcaccgtcc agcagcacgg tgcaacagcc gcagacgccg tgctcgcacc
     7441 ccagccgggt gcccgtcagg tcgagctgct cacggagcag gtcagccagg ctggtcctgg
     7501 ccgccgcacg gacggcacgc ggcgtcccgt tgacgaccac acggatatcg tggacggttc
     7561 ccacggtcac agccgcgcca cggcgtacat cccgctaggc atggcaagaa tctagcggaa
     7621 accgtcattt tgggcaccat ccccccactg cgccgacgcc ggcgtcggct gtaagcgctc
     7681 aacctcgatg ccctcattgc tgaaggtgct cgcggaggaa ctccaaagtc gactgatgca
     7741 ggcgggtgcg gtgcgggacg gtggcccagc cgtggtcggc gtcagggacg acctcgagtt
     7801 ccgcgttgcc gtagtggtcg aggtagcggc gggcgtaggt gaggggggcg atgtcgtcct
     7861 tgtcgccgtg gatgatgcgg acggggccgt gtagtggcgg gcgcggccgt agacgtcgag
     7921 gtcggtgatg tcgtcgacga gggctgggct cacgcggtgg ccgtcggcgt cgaagtatcc
     7981 cttcgcttcg agctgtgcgg cgatgttgcg gcccttcagg tggccgctgc cgatctcgaa
     8041 gggggcgacg gcggcgggtg accagaggca cagggcgcgg attccgggct cttcggcggc
     8101 gacgattccg gcgacgacgc cgcccatgct catcccgagc aggccgatgc gggcgggatc
     8161 ggcgaagtcg aaggctcgaa ctacctgcac cactgagcgt gtttcggcaa tctcgccggt
     8221 gatggtgacg tcgaagaagt cgccgtcgct ctctccgtgg cctgacaggt cgacccggac
     8281 cgaggcgatg ccgttgcgtt ccagcagccg ggacatatgg acgaaggagg agccgaactc
     8341 cagccggttg gcgctgaagc cgtggatgag tatcaccgtc ggccagcggg tcccaggcgg
     8401 gctgtccggg atgtgcacgg tggaccgcag ggttagcccg ctgtgctgga agtcctggtt
     8461 gcggatcacg atgccgcctc tccggccggg acggcggtgg ccaccgggcc ggcggtcagg
     8521 gcccagctgc cgggctcgat gcgccaggcc ttggcctctg cgtcccagtg ggcgagggtg
     8581 cgggcgggca ccagcacctc gacggtggcc gtctcgccgg ggatggcatg ggcaagggcg
     8641 aacccggcga gccagccggg cgggcgcggg agcgtgctgt caggacggga ggcgtagacc
     8701 tggacgacct cgcagccggg acgggagccg gcgttgcgga ccgtgacgtg gacggtgaga
     8761 gtgctgccag ggcccaggtc gccgcccggc gtgagggtgg cgtggccggt ggtcttgacg
     8821 tcgaggtact cccaggtggt gtagccgagg ccatggccga aggggtactg gacggcgcgg
     8881 ccctcggggg cgcggtagcc gacgaagatg ccttcggcat agtccaggcg gccggcggcc
     8941 ggggtggttg tggggaggcc gtccggggag gcgggccagg tggtggggag gcggccaccc
     9001 ggttcgttgg cgccgaggag gacgtcggcg agagcgttgc cgaactcctg gccggggaac
     9061 caggtgagca ggatcgcggg gacctcgtcg gcccagggga gcaggacggg ggcgccggcg
     9121 ttgaccacca cgacggtgtg ggggttggcg gcggcgaccg cgcggaccag gtcgtcctgg
     9181 cggccgggga gggccaggga gttgcggtcg aagccttcgc tctcgacctc gtcggtggtg
     9241 ccgacgacca ccacggcgac gtcggcgtcg cgggccaggg ccaccgcgcg ggcgagctcc
     9301 tcctcgtcgc tgccgcaggg gcggtccacg ttgagctgga aggcggggat tcggctggcg
     9361 tcggtgaggc ggtagctgag ggtgatcggg agctgctggc cagcctccag cgggacccga
     9421 accaggtgct ggggcgggcg catcatggcg ccgatcggct ccacgccggg ttcgggggcc
     9481 agcgggccgg cgaaccgggg cgtcccggcg agcgacagct cgaagtcccc gacaccggag
     9541 cagccgacgg tccagtcgcc gctggcgtcg gccagcagcc gggtacgcac ctcgatggcg
     9601 gcgacccgct cggcagggaa atcgccgaac gagcccagcc aggtgaaggc ggcggtgcgg
     9661 cggtgctcgc ggtgcagcac ggtgccgtcg gcggcgacga agcgcagctc gacaccgccc
     9721 tcctccccct ccggggatga cgccggaggc cgcagcaggc ccaggtcggc gggtgggatc
     9781 ccggtggtgg cccgcgctcc cggcgcgtgg gcgacctcga cctcgggctc cagggcgacg
     9841 cggaggccgt cgagcgggga gatcgcatag ggcgggaaga ccgtggcgct accaccgccg
     9901 agggtgcggg ggacggccgc gttcggcccc agcactgcca cccggcgcag gcggcccgct
     9961 tccaggggca gcacggcgcg ctcgctctcc ccgtcatggt cgcgggcgtg ctcgttgcgg
    10021 accaggacga atcccgcggc ggcggtggcg cgaagctcga cggcgaccgc ggcgctggac
    10081 cagggttccc gctccgcctc cagccgatcg gcgccgaggt cgaggtcgag ggcgccgacg
    10141 cgggcggcca ggcgcagcag gcgcagcacc ttgtcgtcga tggccgcctc gctgacgtga
    10201 ccgtcgctga ccgccgcgac caggtcgtcg ccccacgggc ccgaggggcc gggcataacc
    10261 aggtcgaggc cgctgcgacc gctcgggacg gtcgagcggg tgccgaacca gtcggacatc
    10321 accacgccgt cgaagcccca ctcgtctttg aggacctcgc ggagcagcgg gtgctcgctc
    10381 atggtggtgc cgttgacgct gttgtaggca gccatcaccg cccacgcgcc agcctcccgc
    10441 acgatcgcct cgaagggggc caggtacaac tcacgcaggg tgcgctcgtc gacgcgcaca
    10501 tccacggtat gccgctcggt ctcggagtcg ttggcgacga agtgcttcac cgtcgccgcg
    10561 atcccgccgg actggacgcc gcgcacatag gcggcgccga tccgcgcggt cagcagcgga
    10621 tcctcgctga agcactcgaa gtggcggccg ccgtaggggc tgcggtgcag gttcacggtc
    10681 ggggcgagca gcacctggac gcccttgcgc ctggcctcgg cggccaggag acgaccgagg
    10741 cgctcgaccc gggcctcgtc ccaggtggcg gcgagggcgc tgggggacgg cacgttcgcg
    10801 gacgggtcgc gctcgtccca gcgctccccg cgcacgccag ccgggccgtc ggagaccacc
    10861 acccgccgca gcccgatggc cggttccggg tgcaacgacc agaaaccggc gccagtgagc
    10921 agccgcacct tctgctccag ggtgagcgcc gccagcttgg ccttgagctc cggctccggc
    10981 tccagggtca tccaggcacc tccggggtgg gtcgcccgcg cctgcgcggg aggtaggtcc
    11041 gcagggattg ggtctgccgc aggagggagc gcaggccggg caggtcggcc ggggcggcgc
    11101 gctgcgcccg cgcctgaatc agcaggttgc cgagggcggt cgcctcggct ggcccggcaa
    11161 ccaccgacag ctcgcagacg tccgcggtga gctggcacag caactcgttg cgagcgccgc
    11221 cgccgaccat atggatgacc tcgacgtcgc ggcccgagag ccgctgcgcg tcgtggaggg
    11281 tccggcggaa cgccagcgcg aggctgtcca cgacgcagcg gacggtctcg gccgggctct
    11341 gcggtgctgt ttggccggtg tactcgcacg tggcggcgat ccgcgcgggg aggtcgcccg
    11401 gtgggacgaa gaccgggtcg tccacgtcga tcaccgcgca gaacgccggg aggccggccg
    11461 cctcgcgcag cagcgcctcg aggtcgacgg gccgccctcc gtccgcgtcc gaccagacgc
    11521 gcagcgactc ctgcagcagc cagaggccca tgacgttgcg gagatagcgg atggtgccgt
    11581 ccacccccag ctcgttcgtg aagttggcct gccggctctc ttcgctgagc accggctggt
    11641 ccagctcgac gccgaccagc gcccaggtgc cgcaggagat gtaggcgaag cgctcgccgt
    11701 cggcggggac cccggcgacg gcggaggcgg tgtcgtgcga ccccacggcg gtcaccggca
    11761 cggggccctg gccggcgagc ccgacggcct ccagcacgct gggtagcagc tcgctggcgg
    11821 ggtcgcccgg ttcgcgcagc ggcgggaaca ggccgggagc caggccgagc cggcggatca
    11881 ggtcggcagc ccaggtgcgg gcggtcacgt cgaggagctg ggtggtggag gcattggtaa
    11941 tctcggcccc ctccacccca gtgagccagt aggcgaacag gtcggggatc tgcagcagcc
    12001 gcgccgccgc ctccagctga ggagtccccc tggcggccac cagctggcag agcgtgttga
    12061 acggcaggaa ctgctggccg gtgacggcgt agatgtcggc ggcggggact tcgcgcagga
    12121 ctttgtccag gatcccgtcg gtgcgggcgt ctcggtagtg aacggggttg ccgagcagcg
    12181 ctccggtcgc gtccagcagg ccgtagtcga gcccccacga gtcgatgccg actccgtcga
    12241 gccggccggc ctgccgggcg gcggcgcgca gcccgtcgag gattccctgg tggagggcga
    12301 gcacgtccca gtgcaaggtc tcctgtgccc gcaccgggat gttggggaac cggtggacct
    12361 ccgtgacctc gaagtcgcca ccgccgatgc ggccgagaag gacccggccg ctcgacgcgc
    12421 cgaggtcgat cgctgccagg ttgaccgggc gctccgccgg gttggccgtc atcgcaggaa
    12481 ggcggcggcc acgcccgcgt ccacggggac gagcaggccg gtggtgtggg acagctccgg
    12541 gccggtgagc acgaagacgg cgttggccac gtgctcgggc aggacctccc gaccgagcag
    12601 ggtgcgcttg gcgtagaagg cgcccagctc cgcctgcggc accccgtaga cggcggcgcg
    12661 ctgggcgccc cagccgcccg cgaagatccc cgagccgcgc acgacgccgt cggggttgac
    12721 cccgttgacg cggatcccgt gcgggccgag ctcggcggcg agcaagcgca cctgatgcgc
    12781 ctggtcggcc ttggcggcgc cgtaggcgac gttgtcgggc ccggcgaaca gggcgttctt
    12841 gctgcagatg tagacgaggt caccgccgag gccctgctcg accataatgt gggccgcctc
    12901 gcgggagacc aggaacgagc ccttggccat gacgtcgtgc tggaggtccc agtcgagctc
    12961 ggtggtctcc agcaacggct tggagatcga cagcccggcg ttgttgacca ccaggtccac
    13021 tccgccaaac gccagcaccg tctggcgcag cgcggcggcg accgcgtcgg cgtcggtgac
    13081 gtccacagtc atggcgatgg cgatgtcagc cgggtcgagg acgtggccgt cggcggcgat
    13141 ctcctcggcg accgcgcagg ccgaggcggc gtcgcggtcg gcgacggcga cgcaggcgcc
    13201 ctcggcggca aggcggctgg cgatcgcccg gccgatgccg gagccggcgc cggtgacgaa
    13261 ggcgacgcgg ccggcgagcg gcttgggctt tgggagccgc tggagcttgg cctcctccag
    13321 cgcccagtac tcgatgcgga acttctcgcg ctcgccgatc ggggcatagg acgacagcgc
    13381 ctcggcgccc cgcatcgcgt tgatggcgtt gaggtagaac tccccagcga tccgcgcggt
    13441 ctgtttatca actccgaacg agcacatgcc cacgcccggg accagcacga tcgccggatc
    13501 ggcgccgcgc agcggcggga tgggccctgg ctcctgttcg gcatagcgct cgtagtaggc
    13561 ccggtagtcc tcgcggtaga cggcgtgcag ctcgcgcagg cgagcggcga ccgcctccag
    13621 cggcgcgctg gcgggcagat ccaggaccag cggccgtagc ttggtgcgca ggaagtggtc
    13681 cgggcaggag gtgccgagcg ccgccagcgg ctgcagcttc tcccgggcga ggaagtccag
    13741 gacggcgtcg ctgtcggtgt agtggccgac ccggggccgc gacgtagggg cgtcggtgga
    13801 ggcgagctgg cgcagcagcg gcaacagcgc tgcggcgcgg gcgtgccgct cggccggggg
    13861 cagcggctca aacccggcga cgaccgcgcc gaacgggtcg gccttgccgt gctcgtcgat
    13921 gaagcgctgg gcgctgcgga tgatctccag cgagttggcc tggcacgcgt cgctggtctc
    13981 gccccaggcg gtgatgccgt ggccgcccaa gatcacgccg atggcctgcg gatgggcggc
    14041 cttgacggcg gcgacgtcca gcccgagctg gaagccgggt cggcgccacg gcacccacgc
    14101 cacccgctcg ccgaaacacg ccttggtcag cgcctcgcca tcgacggcgg tggccagggc
    14161 gatgcccgcg tccgggtgca ggtgatcgac gtgggcggcc tccaccaggc cgtgcatggc
    14221 ggtgtcgatc gatggcgccg cgctgccctt gccgtgcagg cagtagtcga acgccgcgac
    14281 catctcgtcc tcgcgctcga cccccgggta gacgccggtc agggcgcgca gccggtccag
    14341 gcgcagcacc gccagcccgc tctcggtcag ggtgccgagg tcgccgccgg aacccttcac
    14401 ccacagcaat tcgaccggct ccccggtgac cgggtcggtc gcggccgcct tgaccgaggc
    14461 gttgccgccc gcgtagttgg tgttggccgg gtcggagccc agccggttac tgcgccccag
    14521 cagcgcggtc acctcaggat gtgccatgag agcctcgctc aggcgtccca gccggcctgg
    14581 gtgccgccga cccgttcggc cacgatccgc tcgccgtacc cggagcggtg gtaggcggcc
    14641 accgggtcgg ggtccaggcc ctgctcttcg cgcaattcgg caagcagcgg ccgcacgtcg
    14701 gtagcgtagg cgtccatcag cacggcgttg gcgcccagca cgtcgccggc cagctgcatg
    14761 ccggccagtg ccgccgcgct gaccagcagc gccttggcgg tcgcctcctg cacgttcatg
    14821 accgagcgga tctgggcggg gatcttcggc tcgatgttgt ggcactggtc gagcatgaag
    14881 gcgatctccc cctggggcgc gtagccgccg ccccggacca cctcgtgcat gatccggaac
    14941 agctggaacg ggtcggcggc gccgaccatc aggtcgtcgt cggcgtagaa gcgggagttg
    15001 aagtcgaacc cgccgagctt gcccgcacgc agcagcaacg ccacgatcat ctcaatgttg
    15061 gtccccggcg cgtggtggcc ggtgtccacc accaccttgg cccggtcgcc cagcgcctgg
    15121 cagtgcagca gcgcggtgcc ccagtccggc acgtcggtcg cgtagaaggc gggctcgaac
    15181 agcttgtact ccagcaacag ccgctgctcg ggcccaagcc gccggtagac cgcggccagc
    15241 gcctcggcga gacgctcctg tcgcgcgcgg atggagtcct ggccgggata gttggtgccg
    15301 tcggcgaacc acagcttcag gtcgcgcgag ccggtggcgt ccatcacctc gacgcacgcc
    15361 agcaggtggt cgagcgcctt gcggcgcacc cgcgggtcgg ggttgcagac gctgccgagc
    15421 ttgtagtcgt cgtcctggaa gacgttggcg ttgatggtgc cgacctgcac gcccaggtcg
    15481 tcggcgtgct tggcgaggtg ggcgtagtcg tcgacggcgt cccaggggat gtgcagcgcc
    15541 acgctcggcg ccaccccggt gaagcggtgg acggtggcgg cgtcggcgac cttctcgtag
    15601 gcgtcgcgcg gcacgcccgg ctgggtgaac accttgaagc gggtgccggt gttgccgaac
    15661 gcccacgacg gcagctcgat gcgctgctgc ctgagcgcgg ccttgacggc aacgaggtca
    15721 cgcatcctac acacccactt cctgatcaat cgattgttgc gcgctggtca tctcgctggc
    15781 cacactagaa attgaattgg tcgatgttgg acttgtcgaa gacctgcggc gggccaagca
    15841 ggatctcccc attggcgccg atcgtcttgg tgccgaggtt gccggccttg aaggtctcgc
    15901 ctggcttgcc ggtgatctgc ccggagcaca gcgccgccgc cgcgtacgcc gccaggtagc
    15961 cgagcttgcc gggatcccat agctcgaacg cctggacggt gccgtccttg acgaacttgc
    16021 gcatctggtt gggggtgccg agcccggtca gcgcgacctt gcccttgaag gaggagccgc
    16081 tgaggtagcg ggcggcggcc gcgacgccga cggtggtcgg cgagatgatc cccttgaggt
    16141 tggggaatgc ctgcagcagg ccctgcgcct gctggaacga ggtctggtcg tcgtcgttgc
    16201 cgtaggcgac cttgaccagc ttcatcttgg cgtactgcgg cttggtcagc tcgttcttca
    16261 tgaacttgat ccaggtgttc tggttggcgg cggtggaggt cgccgacagg atcgcgatct
    16321 ggccgctgta gccgagctgc ttgccgagca cctgcacctc gctgcggccg atgccctcgg
    16381 gggaagcctg gttggcgaac accgtgcgcc cggcagcggc agtgtcggcg tcgtaggtca
    16441 ccaccttgat gccggcggcc atcgcggcct tgagcgcggg cacgaccgcg ttgggatcgt
    16501 cgccggagat cacgatcgcg tcctggtgct gctgggtcag ggtgttgatg taggggacct
    16561 gagccgaggc gctcgcgtcg gagggcccga cctccttgta ggtaccggcg aactcgccga
    16621 ccgcggtctt gccgccgttg tcggaggtgg tgaagtaggg gtatgcagtg ctgcaattga
    16681 tatcagcgct tgtcatcgaa cgtcagttcc gctaggctta ctggcacggg cctctggggc
    16741 cataccgcga cggtcaaggt gtgcctcgac cccaccagtg gatgggcaag agagagctac
    16801 cgactggccg ggcgaccggc ttggcggcaa cggcgcaagc ccgagggatc ccacgacctg
    16861 ggaggcgtcc gggctgcacg ccagtgcgga agacccg
//
LOCUS       DASWDC010000012         7309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_503168, whole genome shotgun sequence.
ACCESSION   DASWDC010000012 DASWDC010000000
VERSION     DASWDC010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503168"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VF995_00355"
     CDS             <1..279
                     /locus_tag="VF995_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342052.1"
                     /translation="KVEPVPFLAVAGLARGARMVAVAAAFAGCGQVARRVSPRRLRPA
                     ADTVAAALTVGGFAVGLWRIVAAWSRPADGDGTGDDDGTGAGRKEGGR"
     gene            279..926
                     /locus_tag="VF995_00360"
     CDS             279..926
                     /locus_tag="VF995_00360"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9342053.1"
                     /translation="MGVYAVKPRFRQLLRGTAKGLANRGVTPDQVTTAGVVASGLGAA
                     ALWLGGRRGWRGAFAAVPVLAFARTAANALDGLVAEESGLGRPAGELYNETADRLGDI
                     AFLAGTATVPGVPPALALGALAAAELSSFVGVGAKAAGGTRRYDGPMGKPDRMLLLGA
                     AGLVAAGLRRPARARVLNAGLALIAVGALATAANRYRRAHAELEARTPAPDRPGA"
     gene            930..1868
                     /locus_tag="VF995_00365"
     CDS             930..1868
                     /locus_tag="VF995_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HEX9342054.1"
                     /translation="MPHNPFANALFWPVAWRVALTLLAGLGGVVLAERRRGRGSLGQL
                     TSSTLFLRVRTWAWIAPLFVLSLFVGGFVVFALAAAIALQGVAEYARLVGLERRYLVL
                     LAAWSVIGLLVASLARRYFLFLPLGCFFLVSLTPILSGEVKGAHRQVSGAIFGYLYIG
                     LPMAYLVFVKAAERWGVGFLLVVGLAVALSDVGAFTVAALKGPKLAPSVSPGKTWSGA
                     AGNLLGAALGVAILWVAVPKVWSALGVAVLVVVIALGAVWGDLIESFVKQDFSVKDAD
                     EALAGFGGVLDRVDSLLVALPLGYYALLVANNLASR"
     gene            complement(1871..2854)
                     /gene="ligD"
                     /locus_tag="VF995_00370"
     CDS             complement(1871..2854)
                     /gene="ligD"
                     /locus_tag="VF995_00370"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005482361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX9342055.1"
                     /translation="MSERAVEITHPDRVLFPDDGITKGELVAYYQDVAEAMLPLVSGR
                     PVTLQRFPNGIGKAGFYQKQVSASTPDWIERVTVPKAEGGTVTHLVPRHAEDLAWVAN
                     QGSVTPHVWCSRVGSLERPDLVVWDLDPATHAGDLPGVLRAARQLRGLLEELDLPAYV
                     KSTGSRGLHVAVPLRPEASTGEAEAFAYDVARALSGRDPERLTTEWLLADREGRLLLD
                     TARNKWAQMIAAPYAVRALPGAPVSLPLAWEELDEPGFDPRRHTLRSVPERLASTNDP
                     WARMEAAAASIGPARERLAARAPDLEPTPPGGVPTRFGRNDRRLRRGTASS"
     gene            2969..4741
                     /locus_tag="VF995_00375"
     CDS             2969..4741
                     /locus_tag="VF995_00375"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX9342056.1"
                     /translation="MEYLIVAQAYDDLERATARLALIDRLAQLFQETPVPLLPTVSLL
                     CQGKIAPDFAGVEIGVAERMAARSVAEATGLTSEQVLARAREVGDLGLATEALLASDP
                     VAAARPATLEVGMVFDTFHEIAASAGQGSQGRKLSLFLELLKRATPLEARYLVRTCTG
                     SLRLGIGTATILDALATVFVDGRRSRPVLERAYNVCSDLGLVAATLAAGGLEAVAGIH
                     VMPGNPVRPMLAQRLSSPAEVLAKLGGSCSAEYKYDGIRVQAHRTADGRLELFTRRLE
                     RISPQFPDVVELLGAGLGPRAAILEGEVVAADPDSGELRPFQEVMFRRRVHGIAEAVR
                     DVPVSMFCFDLLYADGEDLTRLPYLERRARLAQAVTLSPRLRLTTAEQISGDDVETLE
                     LTFQQAVAEGCEGLICKSLAPAASYQAGARGWQWIKLKRDYRTELSDTLDLVVVGALS
                     GRGRRAGIYGALLLACYDPEADVFQTVCKCGTGFSDAELAALPAKLAPLARTEPPARV
                     DARRHADVWFEPGMVVEVVGAELTISPTHTAGWGRVQDDAGLALRFPRFTGRYRDDKS
                     PEDATTVDEVVGMFRAARRAPAGS"
     gene            4780..5493
                     /locus_tag="VF995_00380"
     CDS             4780..5493
                     /locus_tag="VF995_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX9342057.1"
                     /translation="MCGRFTSTSQLSLLAEQFRADPEGAEGHQPSWNVAPATDILVIT
                     ASEDGARRLRSLRWGLVPKWAKDSSSSNQLVNLRAETAGTKPTFKRLLAKQRCIIPID
                     GFYEWQDMGKGQRKQPIYIVARDQQPIALAGLWETWHEKGADDAERLWTCTILTTTPN
                     KLLATIHNRMPVILPPETWDTWLDPASTDADTLAKLLVPAPDDLLMAWPVDPAVGNSR
                     NNRPDLVEPLEGHEPRTAG"
     gene            complement(5515..5991)
                     /locus_tag="VF995_00385"
     CDS             complement(5515..5991)
                     /locus_tag="VF995_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006134347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9342058.1"
                     /translation="MTEPAPLTPRTPRACSIARTLELIGEKWTILVIREVLLGVRRFD
                     EIQRQTGAPRDILSNRLRKLVDVGILERRQYQEHPARSEYRLTTRGYELRPVLLTLHD
                     WGERHLSGDTKPYTWRHRCGEVFQPQLTCAACGEPVTRGRDLSLDPAAEEAPSAVG"
     gene            6098..6661
                     /locus_tag="VF995_00390"
     CDS             6098..6661
                     /locus_tag="VF995_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9342059.1"
                     /translation="MTITAPPMRLHLPTLPAPVRVRPIEPSDGPALQAGLACLSPRSR
                     WLRFHQPLNRFSAAQLRALVEVDHHDREALLAEIEQPDTTWQLIAVARYARVPAPEAE
                     ADQVRAELALVVGDPWHRCGVGRMLVDRLAALARERGIAAFEGELLTENRPMLQFLRE
                     LGPQIGSSVDFRLFGTTAHAVWHLKET"
     gene            complement(6675..6750)
                     /locus_tag="VF995_00395"
     tRNA            complement(6675..6750)
                     /locus_tag="VF995_00395"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6715..6717),aa:Phe,seq:gaa)
     gene            complement(6785..6863)
                     /locus_tag="VF995_00400"
     tRNA            complement(6785..6863)
                     /locus_tag="VF995_00400"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6825..6827),aa:Asp,seq:gtc)
BASE COUNT          934 a         2743 c         2586 g         1046 t
ORIGIN      
        1 aaggtcgagc cggtgccgtt cctggccgtg gccggcctgg cccggggcgc ccggatggtc
       61 gccgtcgccg ccgccttcgc cggctgcggc caggtcgccc ggcgggtctc cccgcggcgc
      121 ctgcgacccg ccgccgacac cgtggccgcc gcgctgaccg tcggcggctt cgccgtgggc
      181 ctctggcgaa tcgtcgccgc ctggtcccgg cccgcggacg gcgacggcac cggagacgac
      241 gacggcaccg gcgccggtcg taaggagggc ggtcgctgat gggcgtctac gcggtgaagc
      301 cgcgcttccg ccagctcctg cgcggcaccg ccaaaggcct cgccaaccgc ggggtcaccc
      361 ccgaccaggt caccactgcc ggggtcgtgg cgagcgggct gggcgccgcc gccctgtggc
      421 tcggcggccg gcgcggctgg cgtggcgcct tcgcggcggt gccggtgctc gcgttcgcgc
      481 gcaccgccgc caacgccctc gacgggctgg tcgccgagga gtccggcctt ggccgccccg
      541 ccggggagct gtacaacgag accgccgacc ggctcggcga catcgccttc ctggcgggaa
      601 cggcgacggt gccgggcgtg ccgccagcgc tcgccctcgg cgcgctggca gcggccgagc
      661 tgtcctcgtt cgtcggcgtc ggcgccaagg ccgccggcgg cacgcgccgc tacgacggcc
      721 cgatgggcaa gccggaccgg atgctgctgc tcggcgccgc cgggctggtc gcggccggcc
      781 tccgccggcc cgctcgggcc cgggtgctca acgccggctt ggcgctgatc gcggtgggcg
      841 cgctggccac cgccgccaac cgctaccgcc gcgcccacgc cgagcttgaa gcgcggaccc
      901 ccgctcccga ccggcccggg gcctgagcgg tgccccacaa ccccttcgcg aacgccctgt
      961 tctggccggt cgcctggcgg gtggcgctga cgctgctggc gggcctcggc ggggtggtgc
     1021 tcgccgagcg gcggcggggc cgcggcagcc tcggccagct caccagctcg accctgttcc
     1081 tgcgggtgcg gacctgggcg tggatcgcgc cgctgttcgt gctgtcgctg tttgtcggcg
     1141 ggttcgtggt gttcgcgctg gccgccgcga tcgcgctgca gggcgtcgcc gagtacgccc
     1201 gcctggtcgg gctggaacgc cgctacctgg tgctgctggc ggcctggtcg gtcatcggcc
     1261 tgctggtcgc gtcgcttgcc aggcgctact tcctgttcct gccgctcggc tgcttcttcc
     1321 tggtcagctt gaccccgatc ctcagcggcg aggtcaaggg tgcccaccgg caggtcagcg
     1381 gcgcgatctt cggctacctc tacatcggcc tgcccatggc ctaccttgtc ttcgtcaagg
     1441 cggccgagcg ctggggggtg ggattcctgc tggtcgtcgg cctggcggtg gcgctctccg
     1501 acgtcggcgc cttcacggtc gccgccctca agggccccaa gctggcgccc tcggtcagcc
     1561 cggggaagac ctggtcgggc gcggccggga atctgctcgg cgccgcgctc ggcgtggcga
     1621 tcctgtgggt ggcggtcccg aaggtgtgga gcgccctcgg ggtggcggtc ctggtggtgg
     1681 tgatcgcgct cggcgccgtc tggggcgacc tgatcgagag cttcgtcaag caggacttct
     1741 cggtcaagga cgccgatgag gccctggccg ggttcggcgg cgtcctcgac cgcgtggact
     1801 ccctcctggt cgccctcccg ctcggctact acgccctgct ggtcgccaac aacctcgcca
     1861 gccgctgagg ctacgaagag gccgtccccc ggcgcaggcg gcggtcgttg cggccgaagc
     1921 gggtcgggac cccgcccggc ggtgttggct ccaggtctgg cgccctcgcg gcgagccgct
     1981 cgcgggcggg gccgatgctg gccgccgcgg cctccatcct cgcccaggga tcattggtgg
     2041 aggccagacg ctccggcacg ctccgcaggg tgtggcggcg cgggtcgaac cccggctcgt
     2101 ccagctcctc ccacgccagc ggcagcgaga ccggcgcgcc ggggagggca cggaccgcgt
     2161 acggcgcggc gatcatctgc gcccacttgt tgcgggcggt gtccagcagc agccggccct
     2221 cgcggtcggc cagcagccac tcggtggtga gccgctccgg gtcgcgaccg gaaagcgccc
     2281 tggcgacgtc ataggcgaac gcctcggcct cgccggtgga cgcctccggg cgcagcggca
     2341 cggcgacgtg cagcccgcgc gacccggtgg acttgacgta ggcgggcaga tccagttcct
     2401 ccagcaggcc ccggagctgc cgggcggccc gcagcacgcc cgggaggtcc ccggcgtggg
     2461 tggccgggtc gagatcccac accaccaggt caggccgctc cagcgagccg acccggctgc
     2521 accacacgtg cggggtgacg ctgccctggt tggcgaccca ggcgagatcc tccgcgtgcc
     2581 gcggcaccag gtgggtgacg gtcccaccct cggccttcgg cacggtcacc cgctcgatcc
     2641 agtcaggcgt gctggcgctg acctgcttct ggtagaagcc cgccttgccg atcccgttcg
     2701 ggaaccgctg cagcgtcacc ggcctgccgc tcaccagcgg cagcatcgcc tcggccacgt
     2761 cctggtagta ggcgaccagc tcccccttgg tgatcccgtc gtccgggaac aacacccggt
     2821 cggggtgggt gatctcaacc gcccgctcgc tcatgccacc gagcgtaccg tgcccggtgc
     2881 cgtcccatcc cgatcagccg gttgtgcctg gctgccgata tcctcagtgg gccgccgcca
     2941 taccgagcgt aagcggaggg agtggccagt ggaatacctc atcgtcgccc aggcgtatga
     3001 cgacctggag cgcgccaccg cccgcttggc gctgatcgac cggctcgccc agttgttcca
     3061 ggagacaccg gttccgctgc tgccaacggt gtcgctgctc tgccagggca agatcgcgcc
     3121 ggacttcgcc ggggtcgaga tcggcgtcgc cgagcggatg gccgcccgat cggtcgcgga
     3181 agccaccggc ctcaccagcg agcaggtcct ggcccgcgcg cgggaggtcg gcgacctcgg
     3241 cctcgccacc gaggcgctcc tagccagtga cccggtcgcg gccgcgaggc cggcgacgct
     3301 ggaagtcggg atggtcttcg acaccttcca cgagatcgcc gcctcggcgg gccagggttc
     3361 ccagggccgc aagctgagcc tgttcctgga gctgctcaag cgggccaccc cgcttgaggc
     3421 ccggtatctg gtccgtacct gcaccggcag cctccggctc gggatcggca cggcgaccat
     3481 cctggacgcg ctcgcgaccg tgttcgtcga cggccgccgc tcccgcccgg tgctggagcg
     3541 cgcctacaac gtctgctccg acctgggcct ggtcgccgcg accctggcgg cgggcgggct
     3601 ggaggcggtg gcgggaattc acgtcatgcc gggcaaccct gtccggccga tgctcgccca
     3661 gcggctctcc agcccggccg aggtgctcgc caaactcggc gggtcctgct ccgccgagta
     3721 caagtacgac ggcatccggg tgcaggccca ccgcaccgcg gacggccgcc tggagctgtt
     3781 cacccgccgc ctggagcgca tctcgcccca gtttcccgac gtggtcgaat tgctgggggc
     3841 cggcctcggg ccgcgtgcgg cgatcctgga gggcgaggtg gtcgccgccg accccgattc
     3901 cggcgagctg cgccccttcc aggaggtgat gttccgccgc cgggtccacg ggatcgccga
     3961 ggcggtgcgc gacgtccccg tgagcatgtt ctgcttcgac ctgctgtatg ccgacggtga
     4021 ggatctcacc cggctgccgt atctggagcg gagggcgcgg ctggcgcagg cggtcaccct
     4081 ctcgccacgg ctgcggctga ccacggccga gcagatcagc ggcgacgacg tcgagacgct
     4141 ggagctgacc ttccagcagg cggtcgccga gggctgcgag gggctgatct gcaagtcgct
     4201 cgcgccggcg gccagctacc aggcgggggc gcgcggctgg cagtggatca agctgaagcg
     4261 cgactaccgc accgagctga gcgacaccct cgacctggtg gtggtcggcg cgctctctgg
     4321 gcggggccgg cgcgcgggga tctacggggc gctgctgctg gcctgctacg acccggaggc
     4381 cgacgtgttc cagacggtgt gcaaatgcgg caccgggttc agcgacgccg aactggccgc
     4441 gctgcccgcc aagctggcgc cgctggcgcg gaccgagccg cccgcccggg tcgacgcgcg
     4501 ccgccacgcc gacgtgtggt tcgagccggg catggtcgtc gaggtggtcg gcgccgagct
     4561 gaccatctcg cccacccaca ccgcgggctg gggccgcgtg caggacgacg ccgggctggc
     4621 tctgcggttc ccgcgcttca ccggccgcta ccgcgacgac aagtcccccg aggacgccac
     4681 caccgtggac gaggtggtcg gcatgttccg ggcggctcgg cgggcacccg ccgggtcctg
     4741 agcgtccaga gtgtcacgcc gggctgctac ggtatgccta tgtgtggtcg tttcacctcc
     4801 acgtcccagc tgtcgctgct ggcggagcag ttccgcgccg acccagaagg ggcggagggc
     4861 catcagccga gctggaacgt cgccccggcg accgacatcc tggtgatcac cgccagcgag
     4921 gacggcgccc gccgcctgcg gtcgctgcgc tgggggctgg tccccaagtg ggccaaggac
     4981 tcctcgtcga gcaaccagct ggtcaacctg cgcgccgaga ccgccggcac caagccgacc
     5041 ttcaagcgcc tgctggccaa gcagcgctgc atcatcccga tcgacggctt ctacgagtgg
     5101 caggacatgg gcaagggcca gcgcaagcag ccgatctaca tcgtcgcccg cgaccagcag
     5161 ccgatcgccc tcgccgggct gtgggagacc tggcacgaaa agggtgccga cgacgccgag
     5221 cgcctctgga cctgcacgat cctgaccacc actcccaaca agctcctcgc cacgatccac
     5281 aaccgcatgc cggtgatcct gccgcccgag acctgggaca cctggctgga ccccgccagc
     5341 accgatgccg acacgctcgc gaagctgctg gtcccggccc ccgacgacct gctgatggcc
     5401 tggccggtgg acccggcggt cggcaactcc cgcaacaacc gccccgacct ggtggaaccc
     5461 ctggagggcc acgagccccg caccgccggc tgagggtgcc tctcgccgtc gagcctagcc
     5521 gacggccgag ggcgcttctt cggccgccgg gtccaggctg aggtcgcggc cccgggtgac
     5581 gggctcgccg caggccgcgc aggtgagctg cggctggaag acctcgccgc aacggtggcg
     5641 ccaggtgtag ggcttggtgt cgccgctgag gtggcgctcg ccccagtcgt ggagggtgag
     5701 cagcactggg cgcagctcgt agcccctggt ggtgaggcgg tactcggagc gggccggatg
     5761 ctcctgatac tggcgccgtt cgaggatccc gacatccacc agcttgcgca gccggttgct
     5821 gaggatgtcg cggggagcgc ccgtctggcg ctggatctcg tcgaagcggc gcacgccgag
     5881 cagcacctcg cggatcacga ggatggtcca cttctcaccg atcagctcaa gcgtgcgcgc
     5941 gatcgagcag gcacgcggcg tgcgcggtgt caaaggggct ggttcggtca tggcgagagc
     6001 gtagcgaatt gggttgtgat ttccaaccct ccagcttaga gtaggttgga ctttccaact
     6061 taccagtcgt agggtcagaa acggaagcgg agtcacgatg acgatcaccg ccccgccgat
     6121 gcgcctccac ctgcccaccc tgcccgcgcc ggtgcgggtg cgcccgatcg agccgagcga
     6181 cggccccgcc ctccaggcgg gcctggcatg cctctcgccc cgcagccgct ggctgcgctt
     6241 ccaccagccc ctcaaccgct tcagcgccgc ccagctccgc gccctcgtcg aggtcgacca
     6301 ccatgaccgc gaggccctgc ttgccgagat cgagcagccc gacaccacct ggcagctcat
     6361 cgccgtcgcc cgctacgccc gcgtccccgc ccccgaagcg gaagcggacc aggtccgcgc
     6421 cgaactcgcc ctggtcgtcg gcgacccgtg gcaccgctgc ggcgtcggcc gcatgctggt
     6481 cgaccgcctc gccgccctcg cccgggaacg cggcatcgcc gccttcgaag gcgagctgct
     6541 gaccgagaac cgcccgatgc tccagttcct gcgcgaactc ggcccccaga tcggctccag
     6601 cgtcgacttc cgcctcttcg gcaccaccgc ccacgcggtc tggcacctga aggagacctg
     6661 atcgagcgat gctgtggtgg gcagggacgg gatcgaaccg ccgaccttgc ggttttcagc
     6721 cgcacgctct accaactgag ctacctgccc aaggcggccc cccgcccgct ggtgcggacg
     6781 ggggtggcgg tcccgacggg atttgaaccc gcgacctccg ccttgacagg gcggcgagca
     6841 cagccaagct gctccacggg acctaggtga cgcctacagc aatctacagc ctttcgtgcc
     6901 cccagcggag ttcgaatccg cgtcgccgcc ttgaaagggc ggggccgggg ctcggcgatg
     6961 ctaccacagc taccccacct gacctgcagc ggcgccacca cgaccggccc ccggccctgg
     7021 tggcgccgcc gcacgcgggc acgcaacggg cacaaccgcg cgccgaggcg ccgtaagagc
     7081 agcgatcggc tcgtttcaac gagcgttgtg cccagcaggc gctccgcgta cggaggcgcc
     7141 gtgacgcctg ccccgtcgcg tagcgcccgc cggccaaggc accggtgcat cgccctggcc
     7201 gcatcgacac cgacgccctc gatgtgccgt tgatgcctgg ccggccgccc agcagccacc
     7261 atcggtcaac ccgtcaccgc cgaccccacc cgctgcggca ccagcgacg
//
LOCUS       DASWDC010000013         6594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_503352, whole genome shotgun sequence.
ACCESSION   DASWDC010000013 DASWDC010000000
VERSION     DASWDC010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503352"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VF995_00405"
     CDS             complement(<1..371)
                     /locus_tag="VF995_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006605817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9342060.1"
                     /translation="MDVQFITSVAVITPDPRVSRRLYIDALGLPLTAAEGDEYLHSED
                     IAGCKHFGVWPLTQAAQACFGTPHWPVDRPVPQASVEFEVADADTVQVAADELKERGF
                     TLLHDARTEPWGQTVARLQSS"
     gene            complement(533..1126)
                     /locus_tag="VF995_00410"
     CDS             complement(533..1126)
                     /locus_tag="VF995_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX9342061.1"
                     /translation="MSTKPAGNEANEAVVIAFRDAAAFEAWLDTHVDLRAGVWLKLAK
                     KGSGIPSLTADEAVDVGLCYGWISGQRKSDDQTYYLQKYVPRRPRSRWSQVNVAKVQE
                     LTAAGRMRPSGLAEVAAAKADGRWAAAYQSQRDATVPSDLAAALAESPRAAQAFDALS
                     KTERYAVILRLVAARTATARTAQLRSAMAALQARGTW"
     gene            complement(1171..1605)
                     /locus_tag="VF995_00415"
     CDS             complement(1171..1605)
                     /locus_tag="VF995_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017585997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9342062.1"
                     /translation="MLRGITTVNLWADDLPAATRWYAELLGAEPYFTSEAAGRGPGYV
                     EFRIGDYQHELGIIDRRFAPPELAAGSAAGPGGAVIYWHVDDVAATLDRLVSLGAKQL
                     EALTERGPGFVTASVIDPFGNVLGIMYNRHYLDILGNLGNRG"
     gene            1784..3370
                     /locus_tag="VF995_00420"
     CDS             1784..3370
                     /locus_tag="VF995_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX9342063.1"
                     /translation="METQERAASVWIVLFDRFIARWLFAFIALSALGLAVLAVTTHGW
                     AHLVLSLPGHLAAALVAGGIALLTRSAARRRSAVVCLLGDLAAVMPNLFGVGGQQLRG
                     LLTSVLLLPVFMPAIRQALRREPVDAIVGGVRPAPPAVAAAARSGVAAQVPAGQVRSF
                     EKPVLVLNPRSGSVPAVRDQLLEAAGRYGVEVREAATPAELVAAARKAVAGGADALGV
                     AGGDGSLAAVAAVAIEAELPFVCVPAGTRNHFARDLGLDRGDPAAAVEAFVAGPERRV
                     DVATVGAQLFLNNASIGAYAALVHEPSYRDDRLRAFDSVLEGMLERDALPIQARFRDG
                     SGNAWDEVLVLFVSNNTYPLTSFGARPRLDAGMLEVSALRRTEGQDLGRALEDLFNGR
                     DQAGNGWAHWTTTSFAVDSPGGKLEVGIDGEPVVLETPIEFRIHAGALRVLLPASGPA
                     SAHPPKSKSPAPRRRGLGLAPTPEGRIEQIAGLVRLGRRLGRVGEREFGPGWDKDGMP
                     AASPPQRRSSSSGSRPDRRP"
     gene            3367..3996
                     /locus_tag="VF995_00425"
     CDS             3367..3996
                     /locus_tag="VF995_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9342064.1"
                     /translation="MIRGRLGRLGREHPGGASVLACGSGIGSLGRAAVRAALRLDQDL
                     FDRAARRHSPLLDQALPCLTRTADYGMLWMAIAGALALAGGPRGRRAAQSGLIAIAIT
                     SALANQLAKRSFDRRRPLLASVPLVRRAQRMPTSASFPSGHAASAAAFATALATTAPA
                     TAVPVGMLAAGVSYSRVHTGVHYPADVAAGALLGFSVGLLVGRRARRRR"
     gene            4114..4998
                     /locus_tag="VF995_00430"
     CDS             4114..4998
                     /locus_tag="VF995_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005170068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX9342065.1"
                     /translation="MRVATWNVNSVKQRVPRLLPWLDQRQPDVVCLQETKLADAAFTE
                     LLGEELARRGYAVALHGQAQWNGVAILSRVGLDDVVAGLAGAPSYGRPQPEARAVAAT
                     CGGIRVYSVYVPNGRTPGSDHYHYKLAWLAALREVVGAGPQPALVCGDMNIAPADADV
                     FDPDAYVGQTHVTPPERAALAELQALGLRDLVRERWPAERVFTYWDYRAGMFHQDLGM
                     RIDLVLASDAVADRARAAWVDRQARKGTGPSDHAPVIVDLDEAPDGDIGPVVPPPSAQ
                     SAKTPSAKRRSAILPQAR"
     gene            complement(5041..6231)
                     /locus_tag="VF995_00435"
     CDS             complement(5041..6231)
                     /locus_tag="VF995_00435"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014443046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9342066.1"
                     /translation="MTCATSQAGLDRLHQAMAAQVANGALPGMVTLIAHGDDVHVDPI
                     GALAFGSPDPMRRDTIFRITSMTKPILAAATMTLVEDGALALDEPIDRLAPELANRRV
                     LKRVDGPLDETVPAGRPITVEDLLTFRMGYGMVFEPSFDPPYPINTAAKKLQLVMGPP
                     DPRTPHPPDEWMRRFGTLPLMYQPGERWQYNAGALVLSVVAARAAGQSLGELFSTRVF
                     EPLGMTDTGFWLPAERTARLPAHYMTNPATGKLELRTLSPPSEWSRPPVFPAGSAGLL
                     STVDDYLAFARLLLNNGVHEGGRLLSEQSVELMTTNHLTPEQRAGAGPVLGGRGWGFG
                     MSVVTTPDEVSPVSGRYGWDGGYGTSWFNDPNRGLIAIAMTQTSDFLFNGGLAEFTRL
                     AMQT"
     gene            complement(6281..>6594)
                     /locus_tag="VF995_00440"
     CDS             complement(6281..>6594)
                     /locus_tag="VF995_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9342067.1"
                     /translation="GLIRSETAADLRTLTATGAGGRTRRTLTGATAGALGLLGALLGT
                     AVAYLAAIAWYRSSLSTTVRHVPVGDLIVILAGLPLAAAIGGWLLAGREPPAFARQPL
                     E"
BASE COUNT          855 a         2335 c         2425 g          979 t
ORIGIN      
        1 tcgctggact gcaatcgagc gacggtttgt ccccacggct cggtacgtgc gtcgtgcagt
       61 agcgtgaagc cccgctcctt caactcgtcc gcagcgacct ggacggtgtc agcgtccgcg
      121 acctcgaact cgacgctggc ctgcgggacc ggtcgatcaa ctggccagtg aggggtgccg
      181 aagcacgcct gggcagcctg agtcaggggc cagaccccga agtgcttgca accggcgatg
      241 tcttcactgt gcagatactc gtcgccctcg gctgcggtga ggggtagtcc aagcgcgtcg
      301 atgtacagcc ttcggctgac ccttgggtca ggtgtgatca ctgcgacgct ggtgatgaac
      361 tgcacatcca tgtcgttacc ctcccggcat tggctcgact ggtctggcga ttgtcgcgcg
      421 ctgctccgac agcggcgctg tcggactccg tcgagagtct cacaggggaa ccgcaccgag
      481 cgggaggccg cgccggcacg aacctttgcc cgcgatgcag ttccaggtca agtcaccagg
      541 tgccgcgtgc ttggagcgcg gccatggcgc tgcggagctg ggcggtgcgg gccgtggcgg
      601 tgcgggcggc aaccagtctc aggatcacgg cgtagcgctc ggtcttgctg agcgcgtcga
      661 acgcctgcgc cgcccgcggg ctctccgcca gggcggcggc gaggtcggat gggacggtgg
      721 cgtcgcgctg cgactggtag gcggcggccc acctgccgtc agccttggcg gccgcgacct
      781 cggccaggcc ggacggtcgc atccgcccgg ccgcggtcaa ctcctggacc ttggccacgt
      841 tgacctgcga ccagcggctg cggggccggc ggggcacgta cttctgcagg tagtaggtct
      901 ggtcgtcgga cttgcgctgg ccggagatcc agccgtagca caggccgacg tcgaccgcct
      961 cgtcagcggt caacgagggg atgcctgatc cctttttcgc gagcttcagc cacacaccgg
     1021 cccgcaggtc gacatgggtg tccagccaag cctcgaacgc cgcggcgtcg cggaacgcga
     1081 tcaccacggc ctcgttggcc tcgttgccgg cgggcttcgt cgacacgcgt gacgcctacg
     1141 gtcggagtcg gagcggggcg tctgctgcgc tcatccgcgg ttgcccaggt tgccgaggat
     1201 gtcgaggtag tggcggttgt acatgatgcc caggacgttg ccgaaggggt cgatgaccga
     1261 ggcggtgacg aacccggggc cgcgctcggt gagcgcctcc agttgcttgg cgcccagcga
     1321 gaccagccgg tccagggttg cggcgacgtc gtcgacatgc cagtagatga ccgccccgcc
     1381 aggacctgcg gcactgcccg cggcaagctc gggcggggcg aaccgccggt cgatgatgcc
     1441 cagctcgtgc tggtagtcgc cgatgcggaa ctcgacgtag cctggcccgc gcccggcagc
     1501 ctcgctggtg aagtacggtt cggcgcccag cagctcggca taccagcggg tcgccgccgg
     1561 caggtcgtcg gcccacaggt tgacggtggt gatgccacgc agcatggtgc cctcctcttc
     1621 ctcacccgca ccggtgatcg ggttcggcga aggtacccgc cacctccgct gacaacaaca
     1681 ccgggccgct cctcggaccg cgctgcgcat cccggcagcg ccgacccgga agccgcggac
     1741 ctggcagttg agttcgtcgc tggaggccgg caacatggct ggcatggaga cgcaggagcg
     1801 agccgcgtct gtctggatcg tgctgttcga ccggttcatc gcccggtggt tgttcgcgtt
     1861 catcgcgctg tcggcgctcg gccttgcggt gctggccgtc acgacccacg ggtgggcgca
     1921 cctcgtactg agcctgccgg gacacctggc cgccgccctg gtcgccggag ggatcgccct
     1981 gctcacgcgc tcggcggcgc gacggcgcag tgcggtggtc tgcttgctcg gcgaccttgc
     2041 cgcggtgatg cccaacctgt ttggggtggg cgggcagcag ttgcgcgggt tgctgaccag
     2101 cgtgctgctc ctgccggtat tcatgcccgc gatccgccag gcgctcaggc gggagcccgt
     2161 cgacgccatc gtcggcggcg tccggcccgc gcctccggcc gtggccgcgg cggcccggag
     2221 cggggtggcc gcgcaggtcc cggcggggca ggtccgcagc ttcgagaagc cggtgctggt
     2281 cctgaacccg cgctccggga gcgtcccagc ggtccgcgac cagcttctgg aggccgccgg
     2341 ccgctacggc gtcgaggtcc gcgaggccgc aacccccgcc gagcttgtcg ccgctgcccg
     2401 caaggcggtc gccggtggcg cggacgcgct cggcgtggca ggcggggacg ggtcgctggc
     2461 ggcggtcgcc gcggtggcga tcgaagccga gttgcccttc gtgtgtgtcc ccgccggcac
     2521 ccgcaaccac ttcgcacgcg acctgggcct ggatcgcggc gacccggccg cggcggtcga
     2581 ggcgttcgtc gccggcccgg agcggcgggt ggacgtcgcc acggtggggg cgcagctgtt
     2641 cctcaacaac gcctccatcg gcgcctacgc cgcgctggtg cacgagccga gctaccgcga
     2701 cgaccggctg cgcgcgttcg acagcgtgct cgaaggcatg ctcgaacgcg acgccctgcc
     2761 gatccaggcg cgcttccgcg acggctccgg caacgcctgg gacgaggtgc tggtcctgtt
     2821 cgtcagcaac aacacctacc cgctgaccag cttcggcgca cggccgcgcc tggacgcggg
     2881 catgctggag gtctcggcgc tgcgccggac cgaaggccag gacctcggcc gcgcgctcga
     2941 agatctgttc aatggccgcg accaggcagg aaatggctgg gcgcactgga ccaccacgag
     3001 ctttgcggtc gattcgcccg gcggcaagct ggaagtcggc atcgacggcg agccggtcgt
     3061 gctggagacc ccgatcgagt tcaggatcca cgccggcgcg ctgcgcgtgt tgcttccagc
     3121 atccgggccc gcgtccgcgc acccgccgaa gtccaagtcc ccggcgcccc ggcggcgagg
     3181 actgggtctg gccccgaccc ctgaagggcg gatcgagcag atcgcggggc tcgtccgcct
     3241 cggccgccga cttggccgag tcggtgagcg cgagttcggg ccgggatggg acaaggacgg
     3301 catgccagcg gcgtcgccgc cgcagcgccg ctccagctcc agcgggtctc ggcccgacag
     3361 gcggccatga tccggggccg cctgggccgc ctgggccgag agcatcccgg cggagcctcc
     3421 gtcctggcgt gcggcagcgg gatcggctcg ctcggccggg ccgcggtgcg ggccgcgctg
     3481 cggctggacc aggatctgtt cgatcgggcg gcacgcaggc actccccgct gctcgaccag
     3541 gcgctgcctt gcctgacccg cacggccgac tacgggatgc tgtggatggc gatagctggg
     3601 gcgttggcct tggccggcgg gccccgtggc cgccgtgccg cccagtcggg tctgatcgcg
     3661 atcgcgatca ccagcgcgct tgccaaccag ctcgccaagc gctccttcga tcgccgccgg
     3721 ccgctgctcg cctcggtccc actcgtccgc cgcgcccagc gcatgcccac ctcggcctcg
     3781 ttcccctccg gccacgccgc ctcggcggcc gccttcgcca ccgccctcgc caccaccgca
     3841 ccagcgacgg cggtcccggt cgggatgctg gcagccgggg tttcctactc ccgggtccat
     3901 accggcgtgc actacccggc cgacgttgcc gcaggcgccc tcctcggctt cagcgtcggc
     3961 ctcctggtcg gccgccgtgc ccgccgcagg cggtgatcac cgcgccaccg atctctcccg
     4021 gctcgcgctc ggccgggaac aggctgggcc cgtttcgggg ccggaggagc tgcggcttcg
     4081 gcaactgtcg tcctctccgg ctattgtggc cgggtgcgag tcgcgacctg gaacgtcaac
     4141 tccgtcaagc agcgggtgcc ccggttgctg ccctggctgg atcagcggca gcccgatgtg
     4201 gtctgcctgc aggagaccaa gctcgccgac gccgcgttca ccgagctgct cggcgaggag
     4261 ctcgcccgcc gcggctacgc ggtcgcgctg catggacagg cgcagtggaa cggcgtggcg
     4321 atcctctcca gggtggggct ggacgacgtc gtggcgggcc tcgccggcgc gccctcctac
     4381 ggccgcccgc agccagaggc ccgcgccgtg gccgcgacct gcggcgggat ccgggtctac
     4441 tcggtgtatg tcccgaacgg gcggacgccg ggctccgacc attaccacta caagctggcc
     4501 tggctggccg cgctgcggga ggtggtcggc gccggcccgc aaccggcgct ggtctgcggt
     4561 gacatgaaca tcgcgccagc cgacgcggac gtgttcgacc cggacgccta tgtcggccag
     4621 acgcacgtca cgccgccgga gcgggcggcg ctggcggagc tgcaggcgct cggcctgcgc
     4681 gacctggtcc gggagcgctg gccggccgag cgggtgttca cctactggga ctaccgcgcc
     4741 ggcatgttcc accaggacct ggggatgcgg atcgacctgg tgctggcctc cgacgctgtc
     4801 gccgaccggg cgcgggcggc atgggtggac cggcaggcgc gcaagggcac cgggccgagc
     4861 gaccacgccc ccgtgatcgt cgacctggac gaggcccccg acggcgacat cgggccggtc
     4921 gtgccgccgc cgtccgcaca gtccgccaag acgccgtccg cgaagcgacg ctcagccatc
     4981 ctgccccagg ctcgctgacc gcaggcccga gcccttggag cgcgccacgg gaggggtcgg
     5041 tcaggtctgc atcgccagcc tggtgaactc ggcgaggccg ccgttgaaca ggaaatcgga
     5101 ggtctgcgtc atggcgatgg cgatcaggcc tcggttgggg tcgttgaacc acgaggtgcc
     5161 gtagccgcca tcccagccgt agcggcccga aacgggcgac acctcgtccg gtgtggtgac
     5221 tacggacatg ccgaaccccc agccgcgccc gccgagcacg ggcccggcgc cggcccgctg
     5281 ctccggcgtc aggtggttgg tcgtcatcag ctcgacggac tgctccgaca gcagccgccc
     5341 gccttcgtga acgccgttgt tgagcagcag ccgggcgaat gccaggtagt cgtcgactgt
     5401 cgacagcaga cccgccgagc cggccgggaa taccggcggt ctgctccact cgctcggcgg
     5461 cgagagcgtc cgcaactcca gcttcccggt tgccgggttc gtcatgtagt gggccggcag
     5521 ccgggcggtc cgctcagcgg gcagccagaa gccggtgtcg gtcatgccaa gcggctcgaa
     5581 gacgcgggtg ctgaacagct cccccagcga ctggcccgcg gcgcgcgcgg ccaccacgct
     5641 cagcaccagc gcgccggcgt tgtactgcca ccgctcgccg ggttgataca tcagcggcag
     5701 ggtcccgaag cgccgcatcc actcgtccgg ggggtgcggc gtccgcggat ccggcggccc
     5761 catgacaagt tgcagcttct tggcggcggt gttgatcggg tagggcgggt cgaagctggg
     5821 ctcgaagacc atgccgtagc ccatgcggaa ggtcagcagg tcctccaccg tgatcgggcg
     5881 gccggccggg acggtctcat cgagcgggcc gtcgacgcgc ttgaggaccc ggcggttggc
     5941 cagttccggc gccagccggt cgatcggctc gtccagcgcc agcgccccgt cctcaaccag
     6001 cgtcatcgtc gccgcggcca ggatcggttt ggtcatggag gtgatccgga agatggtgtc
     6061 gcgccgcatc gggtcgggac tgccgaacgc cagcgcgccg atcgggtcga cgtgcacgtc
     6121 gtcaccgtgg gcgatgagcg tcaccatgcc gggcagcgca ccgtttgcga cctgagccgc
     6181 catcgcctgg tgcaggcggt cgaggccggc ctgcgacgtc gcgcatgtca tcttcggaac
     6241 ccatgctcgc ggattcggtg tggtctcgca tcccccctgg tcattcgagc ggctggcggg
     6301 cgaaggcggg cggttcgcgc ccggcgagca gccagccgcc gatggcggcg gcgagcggca
     6361 ggccggcgag gatgacgatc aggtcgccga ccgggacgtg gcggacggtg gtggagaggc
     6421 tgctgcggta ccaggcgatc gcggcgaggt aggcgacggc ggtcccgagc agggcgccga
     6481 gcaggccgag ggcgccggcg gtggcgccgg tcagggtgcg gcgcgtcctg ccgccggcgc
     6541 cggtggcggt gagcgtgcgc aggtcagcgg cggtctcgct gcggatcagg ccga
//
LOCUS       DASWDC010000014         7158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1589206, whole genome shotgun sequence.
ACCESSION   DASWDC010000014 DASWDC010000000
VERSION     DASWDC010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589206"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..443)
                     /gene="secE"
                     /locus_tag="VF995_00445"
     CDS             complement(111..443)
                     /gene="secE"
                     /locus_tag="VF995_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX9342068.1"
                     /translation="MNRETKRAMARQQRTADPARLQQLRRQQVAARQRDARAGTTAPG
                     KGKSRMQGVSSFGGEVVSELKKVNWPDRRTVMGYTVVVLVSVTVITAIIFGLDTLFGK
                     AALWIFTR"
     gene            complement(534..609)
                     /locus_tag="VF995_00450"
     tRNA            complement(534..609)
                     /locus_tag="VF995_00450"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(574..576),aa:Trp,seq:cca)
     gene            complement(692..1924)
                     /locus_tag="VF995_00455"
     CDS             complement(692..1924)
                     /locus_tag="VF995_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342069.1"
                     /translation="MPGIHFLDVTNRDGVQTARVNLSKFGKTMVNFYLGKLGVAQSEL
                     GFPFLFHEEPYIRANLALAKAGAFGSLRLSGWTRALAGDVERALPLGLKHLNLSISTS
                     DQMIQHKFRGRLDRDAVIREMTAATRAARQGAAETVGVNAEDGSRTDDGYLLEFAQAA
                     REAGADRVRYCDTIGGDTPERIRERFAKLAGAVGIPVETHCHNDLGMAVANSVAGALG
                     DLDAGQDAWVNTCVNGIGERAGNADLVSCILAFQHAFALAGRVQIADSIDLSWARRFA
                     LWASDAVGQPLPLGQPGVGRNAFSHESGIHADGALKDRHNYELYDDDTLGPFPHDWAN
                     REGRVVLTGEYGGKAGFRHVMDGLGVPVTPADEDLVFKLVQLSNAATGRPLHDDELRL
                     LASYPHELALLYPGSGLI"
     gene            2054..3433
                     /locus_tag="VF995_00460"
     CDS             2054..3433
                     /locus_tag="VF995_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX9342070.1"
                     /translation="MEGRIGAAEAARKLGIKPESLYAYVSRGLLTRERGPDGRTSWFD
                     PAEVARLAARSRHPRPPRQGGAELAIESALTTIEGGRLWYRGCDATRLATDHPFERVA
                     WLLWTGTLPGTTTDPRQHPWPGSWSRSWHAGQSGVAVGSAAQAALPPGTLPLERLRVI
                     AAALATTDALRFELNPTAVLATAPSLLTGLVECLPSPVDNASTPPLESDRLTSGGPPG
                     RGAASISMPISMPISIAARLWGKLCAAPPHPDFVALLDDALVLLADHELAASTLAARV
                     AASVRADPYAVVSVGLGAVSGALHGAVSLAVEDLLAEVGGPAAAKAAARAEAAAARAV
                     GERLRRGERLRGFGLALYPDGDPRAAFLLERLRAIGGGAAASARLAAVEAVLDTTRRL
                     GLPPPNVDFALGALAMVTGMIRGAGEAIFAVARTAGWLAHALEEYDRGQPLRPRALYT
                     GPPPAADAT"
     gene            complement(3426..3845)
                     /locus_tag="VF995_00465"
     CDS             complement(3426..3845)
                     /locus_tag="VF995_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342071.1"
                     /translation="MMGCHDIRQLLPARLEELTPLEARARALHVAGCARCAAEAASYS
                     RLVQTLATMANLEATPPDGTVERILVAIGRRRVRATDPRVMVAAGAGGALVATVMVAR
                     ALRQRRRVPAAAAPGRARAALPSVVSAGQTAVLARLK"
     gene            complement(3842..4450)
                     /locus_tag="VF995_00470"
     CDS             complement(3842..4450)
                     /locus_tag="VF995_00470"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9342072.1"
                     /translation="MEVDRELVEAAQAGDRWALEELVRRTHRSVYTCALRLVGNPDDA
                     ADVTQDVYLRVVRKLGGFRHEASFATWLNRVTTNVAMSSLKRRTRRIAVEGAMVPLET
                     RDPAPDPSERAEAVLLARRLEKLVAGLPEGQRQVLVLRDVYGQSTDEVATAMGLTPGA
                     VKVRLFRARERLKAELGATEGGPCQGQVVNLAGRRRASGRRS"
     gene            complement(4578..5612)
                     /locus_tag="VF995_00475"
     CDS             complement(4578..5612)
                     /locus_tag="VF995_00475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9342073.1"
                     /translation="MSRARRDVPQGVLDLVVERQAAREARDFARADALRDQVRAAGWL
                     LRDTREGPELAAAPPWEPVDPAAIRPRWDEPDQHAVSVIAHVTGWPEDVARALHALAT
                     HCAPRAAPRAVPLDYEVLLLDDGADAATGQALEDLAEEDPAGRVRVLHLNPSAEHASE
                     RGDAVDGRSAEAIGFGAAMNLGMGQATGRVLVWLDPHVEATGDLLTPLLSALDEPGVG
                     LAGGWGVVTRSMLQFEADDGPEVDAVEGYLLAVPRRLAARVEVDPKDRYYRNADLDYS
                     FAIRALDPGYRAIRLAVPATLHRHRGWYETDPAERERASKKNYDRFLAKWRLRTDLLT
                     RGFTGYHQHG"
     gene            complement(5596..6174)
                     /locus_tag="VF995_00480"
     CDS             complement(5596..6174)
                     /locus_tag="VF995_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9342074.1"
                     /translation="MRLDPQKVQPGQALPEVSHTIDRIDLIRYAGASGDFNPIHWNDE
                     VAKAVGLPGVIAHGMFSMGTATRVITAFAGDPGAIKRLRVRFSAMIQPGQTLTAKGEV
                     TGVDGDRVTIRFWAEDEHGEKVVAKGEAELDLGPGGSGGAVPRPEAVRAMTMAAAGPP
                     DESGDSGGAVPRPEGLQAGSTATAGPPDESGA"
     gene            complement(6171..6656)
                     /locus_tag="VF995_00485"
     CDS             complement(6171..6656)
                     /locus_tag="VF995_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9342075.1"
                     /translation="MPLNQSLVGKEYPPVVYEVGREKLREFAAAVGETDPVFHDEEAA
                     RAAGHADIVAAPTFPVVVSLQATAALHGDPELGLDYSRVVHGEQEFSYARPIVAGDRL
                     LASSRVAAAEVKGRHELLTIETRVTTEGGDPVCTVRNVLLSRGTAPERDRQPQPREAA
                     T"
     gene            complement(6711..6875)
                     /gene="rpmG"
                     /locus_tag="VF995_00490"
     CDS             complement(6711..6875)
                     /gene="rpmG"
                     /locus_tag="VF995_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX9342076.1"
                     /translation="MATTDTRPKITLACTVCKHRNYTTTKNRRNDPDRIELKKYCRWC
                     GRHTAHRETR"
     gene            complement(7018..>7158)
                     /locus_tag="VF995_00495"
     CDS             complement(7018..>7158)
                     /locus_tag="VF995_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX9342077.1"
                     /translation="EMAMPGDNTEMVVELISPIAMQEGLRFAIREGGRTVGAGRVVKI
                     LK"
BASE COUNT          947 a         2636 c         2505 g         1070 t
ORIGIN      
        1 gacataccag tccccgagac tctggttgaa gtcggggctg gcttcgccgg tgacaatgtc
       61 agtactcatg gcactcccgg tgtttccttc gtggccctcc ggttcggcgg tcaccgtgtg
      121 aatatccaga gggccgcctt cccgaacagg gtgtccaacc cgaagatgat ggcggtgatc
      181 acggtcaccg agaccagcac gaccacggtg tatcccatga ccgtgcgccg gtcgggccag
      241 ttcaccttct tcagctcgct gacgacctcg ccgccgaacg agctgacgcc ctgcatccgg
      301 gactttccct tgcccggagc ggtggtgcca gcgcgcgcgt cccgctgccg ggccgcgacc
      361 tgctgcctgc gtagctgctg caggcgcgcc gggtcggcgg tgcgctgctg gcgagccatc
      421 gcccgcttgg tctccctgtt catcgcaagc cttgctcctc gcgccgggtg gtgcagcaga
      481 tcatcggtcg gtacgaccca acccttgccc cacgaagacg gatgacaaga ggctggcagg
      541 ccaggaggga ctcgaacccc caaccaccgg ttttggagac cgggactcta ccaattgagc
      601 tactggccta tgcctggcca cgccccggac ccgggaccaa agccagacga gtatacgggg
      661 gcactcccgc cgctctcaag gtcggagcgg gtcagatcag accgctacca ggatatagca
      721 gcgccagctc atgcgggtag ctggcgagca ggcgcagctc gtcgtcgtgc agcggccggc
      781 cggtggcggc gttgctgagc tgcacgagct tgaagacgag gtcctcgtcg gcaggggtga
      841 ccgggacgcc gagcccgtcc atgacgtgcc ggaagcccgc tttgccgccg tactcgccgg
      901 tcagcaccac ccggccttcc cggtttgccc agtcgtgcgg gaatggcccg agcgtgtcgt
      961 cgtcgtagag ctcgtagttg tggcggtcct tgagcgcccc gtcggcgtgg atgcccgact
     1021 cgtgggagaa agcgttgcgg ccgacccccg gctggccgag cggcagcggc tggccaacgg
     1081 cgtcgctggc ccacagggcg aaccgccgcg cccaggagag gtcgatcgag tcggcgatct
     1141 ggacgcgccc ggccagcgcg aacgcgtgct ggaacgcgag gatgcaggaa accaggtcgg
     1201 cgttgccggc gcgctcgccg atgccgttga cgcaggtgtt cacccaagcg tcctgcccgg
     1261 cgtcgaggtc gccgagcgcg ccggcgaccg agttggccac ggccatgccg aggtcgttgt
     1321 ggcagtgcgt ctcgaccggg atcccgaccg cgccggcgag cttggcgaac cgctcgcgga
     1381 tgcgctccgg ggtgtcgccg ccgatggtgt cgcagtagcg gacccggtcg gcgccggcct
     1441 cccgggcggc ttgggcgaac tccagcaggt agccgtcatc ggtgcgcgac ccgtcctcgg
     1501 cgttgacccc gaccgtctcg gcggcgccct gcctggccgc ccgggtcgcg gcggtcatct
     1561 cccggatgac ggcgtcccgg tcgagccgcc cgcggaattt gtgctggatc atctggtccg
     1621 aggtcgagat cgagaggttc aggtgcttga ggccgagcgg cagggcccgc tcgacgtcgc
     1681 cggccagcgc ccgggtccag ccggacagcc gcaggctgcc gaacgcgcca gccttggcca
     1741 gcgcgaggtt cgcccggatg tagggctcct cgtggaacag gaacgggaat cccagctccg
     1801 actgggcgac cccgagcttg cccagataga aattgaccat cgtcttgccg aacttcgaca
     1861 ggttgacccg ggccgtctgc acgccgtccc ggttggtcac gtcaaggaaa tggatgcccg
     1921 gcatgctctg cctccagctc cggcggtggt acggctgcgc tcagccacgc ctcaagttga
     1981 ctcctccact tggggtacgt caatgttgat caataatcaa tgttggagtg ggagctggag
     2041 gctggagccg cggatggagg ggcgcatcgg ggccgccgag gcagcgcgca aactcggcat
     2101 caagccagaa agcctctacg cctacgtgag ccgcggcctg ctcacccgcg agcgcggccc
     2161 cgacggccgc accagctggt tcgaccccgc cgaggtcgcg cgcctcgccg cccgcagccg
     2221 ccacccccgc cccccacggc agggcggcgc cgagctggcg atcgagtccg ccctcaccac
     2281 gatcgagggc ggccgcctct ggtaccgagg ctgcgacgcc acccgcctcg ccaccgacca
     2341 ccccttcgaa cgggtcgcct ggctgctgtg gaccggcacg ctccccggca ccaccaccga
     2401 cccccgccag cacccctggc ctgggtcctg gtccaggtcc tggcacgcgg gccagtccgg
     2461 cgtcgcggtg ggaagcgccg cccaggcggc cctccccccg ggcaccctgc cgctcgaacg
     2521 cctccgcgtg atcgccgccg ccctcgccac caccgacgcg ttacgcttcg agctgaaccc
     2581 caccgccgtc ctcgccacgg ctccgtccct gctcacaggc ctggtcgagt gccttccaag
     2641 ccctgtggac aacgccagca cgccccccct cgaatccgat aggctgactt cgggggggcc
     2701 cccggggagg ggcgccgcgt cgatttcgat gccgatttcg atgccgattt cgatcgccgc
     2761 ccggctctgg ggcaagctct gcgccgctcc gccgcatccc gacttcgtgg cgttgctcga
     2821 cgacgcgctg gtactgctcg ccgaccatga actggctgcg tcgaccctgg ccgcgcgggt
     2881 ggccgcctcg gtccgggctg acccctacgc ggtcgtgtcg gtggggctcg gggcggtgag
     2941 cggcgccctg cacggggcgg tgtcgcttgc cgtcgaggac ctgctcgccg aggtcggcgg
     3001 acccgccgct gccaaggccg ccgccagggc cgaggccgcc gcagcccgag ccgtcggcga
     3061 gcggctccgg cgcggcgagc ggctccgcgg cttcggcctc gccctctacc cggacgggga
     3121 cccgcgtgcg gcgttcctgc tggaacggct gcgcgccatc ggcggcggag ccgcggcctc
     3181 cgcccggctg gccgccgtgg aggcggtgct cgacaccacc aggcggctcg gcctgccccc
     3241 gccgaacgtc gacttcgcgc tcggcgcgct cgccatggtc acgggcatga tccgcggcgc
     3301 gggcgaggcg atcttcgcgg tcgcgcggac ggccgggtgg ctcgcccacg cgctggagga
     3361 atacgaccgc ggccagccgc tgcggccgcg cgcgctctac acgggcccgc cgcccgcggc
     3421 ggacgctact tgagccgcgc caggacggcg gtctgaccgg ccgacaccac cgacggcagg
     3481 gcggccctgg ccctccccgg cgcggctgcg gccggcacgc ggcggcgctg gcgtagggcg
     3541 cgggccacca tcacggtcgc gaccagggcg ccgccggcgc cggcggcgac catgacccgc
     3601 gggtcggtgg cgcggacccg gcggcggccg atcgccacca ggatgcgctc cacggtgccg
     3661 tcgggcgggg tcgcctccag gttggccatg gtggcgaggg tctggaccag tcggctgtag
     3721 gaggccgcct cggcggcgca gcgggcgcag ccggccacgt gcagggcgcg ggcgcgggcc
     3781 tccagcggag tcagctcctc cagccgcgcc ggcaggagct ggcggatgtc gtggcagccc
     3841 atcatgaccg ccgccccgac gcgcgccggc gtccggcgag gttgacgacc tgcccctggc
     3901 agggcccgcc ttcggtggcc cccagctcgg ccttgagccg ctcccgtgcc cggaacagcc
     3961 gcaccttcac cgcgcctggg gtcagcccca tcgcggtcgc cacctcgtcg gtcgactgcc
     4021 cgtagacgtc acgcagcacc agcacctggc gctgcccctc gggcaggccc gcgaccagct
     4081 tctccagccg gcgggcgagc aggaccgcct cggcccgttc ggaggggtcc ggtgccgggt
     4141 cgcgcgtctc gagaggcacc atcgcgccct cgaccgcgat ccgccgcgtc cgccgcttca
     4201 aactgctcat ggccacgttc gtcgtcaccc gattcaacca ggtggcgaac gaggcctcgt
     4261 gccggaagcc gcccaacttt cgcaccaccc ggaggtagac gtcctgggtc acgtcggcgg
     4321 cgtcgtccgg gttgcccacc aacctgagcg cgcaggtata cacgctccgg tgcgtgcggc
     4381 gcaccagctc ctcaagggcc cagcggtcac ccgcctgcgc ggcctcgacg agctctcggt
     4441 cgacctccac ctcgagtccc cttactgctt ggcgaacgta ctgacctcga cgggaccggt
     4501 tcggttaccg gctcgggtac cggctcaacg gctggtttcg gctgctggtg gcgggcgttc
     4561 aggctgaaac aacgatccta cccgtgctgg tggtacccag tgaagcctcg ggtcagcaga
     4621 tccgtacgca acctccattt ggcgaggaag cgatcataat tcttcttcga ggctcgctcc
     4681 cgctcggcgg gatcggtctc gtaccagcca cggtggcggt gcagagtggc ggggaccgcc
     4741 agccggatcg cccggtagcc ggggtcgagc gcgcggattg cgaacgagta gtcgaggtcg
     4801 gcgttgcggt agtagcggtc cttcgggtcg acctcgaccc gggcggcgag ccgcctgggc
     4861 accgccagca ggtagccctc gaccgcgtcc acctccgggc cgtcatcggc ctcgaactgg
     4921 agcatcgagc gggtcaccac cccccagccg ccggccaggc ccacgcctgg ttcgtcgagg
     4981 gcggacagca gcggggtgag caggtccccg gtcgcctcca cgtgcgggtc gagccagacg
     5041 aggacgcggc cggttgcctg gcccatgcca aggttcatgg cggcgccgaa cccgatcgcc
     5101 tccgccgacc tcccatcgac ggcatcgcct cgctcgcttg catgctcggc cgacggattc
     5161 aggtggagga ctcgcacccg cccagccggg tcctcctcgg cgaggtcctc cagggcctgg
     5221 ccggtggcgg cgtcagcgcc gtcgtcgagc agcaggacct cgtagtccag cgggaccgcc
     5281 cgcggggccg cccgcggggc gcagtgggtg gcgagtgcgt gcagcgcgcg ggcgacgtcc
     5341 tcgggccagc cggtgacgtg cgcgatcacg ctgaccgcat gctggtccgg ttcgtcccag
     5401 cgagggcgga tcgcggccgg gtcgacaggc tcccagggtg gcgccgcggc cagctccggc
     5461 ccctcgcggg tgtcccggag cagccagccg gcggcgcgga cctggtcgcg cagggcgtcg
     5521 gcacgggcga aatcgcgcgc ctcgcgggcc gcctgccgct ccacgaccag gtcgaggacc
     5581 ccctgcggta cgtccctacg cgcccgactc atcgggaggc cccgcggtcg ccgtggaacc
     5641 ggcttgcagt ccttccggcc gcgggaccgc gccccccgaa tcccctgatt catcgggggg
     5701 ccccgcggct gccatggtca tcgcccgcac tgcttccggc cgcgggaccg cgccccccga
     5761 cccccccggt ccaaggtcca gctcagcctc tcctttggcg acgaccttct cgccgtgctc
     5821 atcctcggcc cagaaccgga tcgtcacgcg gtcgccgtcg acgccggtca cctcgccttt
     5881 ggcggtgaga gtctggccgg gctgaatcat cgccgagaac cggacccgca gccgcttgat
     5941 cgcgcccggg tcgccggcga acgccgtgat gacgcgggtc gccgtgccca tcgagaacat
     6001 gccgtgggcg atcactcctg gcaacccgac cgccttggcg acctcgtcgt tccagtggat
     6061 cgggttgaag tcgccggaag cgcccgcgta cctgatgaga tcgatccggt cgatggtgtg
     6121 cgagacctcg gggagcgcct gccccggctg gactttctgg gggtcgagcc tcacgtcgcc
     6181 gcctcccggg gttgcggctg ccggtcccgt tcgggggcgg taccgcgtga cagcagcaca
     6241 ttgcggaccg tgcagaccgg gtcgccgcct tcggtggtca cccgggtctc gatggtgagc
     6301 agctcgtggc ggcctttcac ctcggcggcg gcgacgcggc tgctggcgag cagccggtcc
     6361 ccggccacga tcgggcgggc ataggagaac tcctgctcac cgtggaccac tctggagtag
     6421 tcaaggccga gctcggggtc gccatggagg gccgcggtcg cctgcagcga caccaccacc
     6481 gggaaggtgg gcgccgccac gatgtcggcg tggccggcgg cccgtgcggc ctcctcgtca
     6541 tggaagacgg ggtcggtctc ccccaccgcc gcggcgaact ctcggagctt ctcgcggccc
     6601 acctcgtaga cgaccggcgg gtactccttg ccgaccaggg actggttcaa gggcatggcc
     6661 gctccctcgc cgtgagtgcc atggttggtt gccgtgcggg gttgcgcggg tcagcgggtc
     6721 tcgcgatggg cggtgtggcg cccgcaccag cggcagtact tcttcagctc gatccggtca
     6781 gggtcgttgc gccgattctt ggtggtggtg tagttgcggt gcttgcacac cgtacaggcc
     6841 agggtgatct tgggtcgggt atcggtcgtc gccatcggtg gatccttcct tcaggtatcg
     6901 gcggccccca aaggccccca aagatgcgtg ctgtagaggg gagcctacgc gagttcgcgc
     6961 caccctagca ggccccgacc gcaccgaggc ggccggggcc agttcgcgga ccgtggacta
     7021 cttgaggatt ttgacgacgc ggccggcacc gacggtgcgg ccaccctccc ggatcgcgaa
     7081 ccgcagcccc tcctgcatcg cgatcggact gatcaactcc accaccatct cggtgttatc
     7141 ccccggcatc gccatctc
//
LOCUS       DASWDC010000015         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725 k141_3300,
            whole genome shotgun sequence.
ACCESSION   DASWDC010000015 DASWDC010000000
VERSION     DASWDC010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3300"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VF995_00500"
     CDS             <1..822
                     /locus_tag="VF995_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007460453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX9342078.1"
                     /translation="ANTASLLLPVSNLTNLLAANRVALPPVAFAARMLLPELAAVTAT
                     AACLWVFYWRRGRRHNPGPGPGPGGRYTPPPPHVPADPVLFAAAAIACAVFASCVLAG
                     VPLALATLGCAGLVAVAFRLRAPSLLRPALLPWRLLVFVTGLFLVVQTVSRHGLGTVV
                     GHAVGAATGTPGILHAALTGAVLSNLVNNLPTYVAGEAAVPLTHGTQLLGLLIGTNVG
                     PIVTPWASLATLLWHERCRADGLHISWRAFTLTGIVTATCVLAAALCGLLVTSPA"
     gene            1004..1201
                     /locus_tag="VF995_00505"
     CDS             1004..1201
                     /locus_tag="VF995_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342079.1"
                     /translation="MLRIIRRAAIESGIDFTLKRQGRAHEVWLCGQTLLVVPRHRNLN
                     QLTAQSIRKKLESEFAKDWWK"
     gene            1198..>1524
                     /locus_tag="VF995_00510"
     CDS             1198..>1524
                     /locus_tag="VF995_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9342080.1"
                     /translation="MSEHRYRVVAERSGDWWAISVPDLPGVFSQARRLDQVDAMAREA
                     IALMLDVDETAVAVTIDPTLPPRTAAAVSEALSRRQARRDAEAREQEALRQAAGALAA
                     DGFTQRE"
BASE COUNT          224 a          622 c          443 g          235 t
ORIGIN      
        1 gccaacaccg ccagcctgct gctgcccgtc tccaacctca ccaacctgct ggccgccaac
       61 cgggtcgcgc tgccgcccgt tgccttcgcg gcacggatgc tgctccccga actcgccgcg
      121 gtcaccgcca ccgccgcctg cctctgggtc ttctactggc gccgaggtcg ccgccacaac
      181 cccggccccg gccccggccc cggcggccgc tacactccgc cgccgccgca cgtccccgcc
      241 gacccggtcc tgttcgcggc ggcggccatc gcctgcgccg tcttcgcctc ctgcgtcctc
      301 gccggcgtcc cgctggcgct cgcgaccctc ggctgcgccg ggctggtcgc tgtcgccttc
      361 cgcctccgcg ccccgagcct gctacgcccc gcgctgctcc cctggcggct gctggtgttc
      421 gtcaccgggc tgttcctggt cgtccagacc gtcagccgcc acggcctcgg caccgtcgtc
      481 ggccacgccg tcggcgccgc caccggcacc cccggcatcc tccacgccgc cctcaccggc
      541 gcggtcctct ccaacctggt caacaacctc cccacctacg tcgctggcga agccgccgtc
      601 cccctcaccc acggcaccca gctcctcggc ctcctgatcg gcaccaacgt cggcccgatc
      661 gtcaccccct gggcctccct cgccaccctc ctctggcacg aacgctgccg cgccgacggc
      721 ctccacatct cctggcgcgc cttcaccctc accggcatcg tcaccgccac ctgcgtcctc
      781 gccgccgccc tctgcggcct cctcgtcaca agcccggcct gacgcccgga gttcggtctc
      841 gagcctgtgg ataacgtcag cagcctcggt ccggaacagc catgctacgg cggatcgatg
      901 gcacagccat cgcggagcct tcggagggat gcatgagtat ggcaattaga ttagccaaac
      961 gattggcaaa tggattggaa aacgggcgat gctccgcgta gacttgcttc ggatcattcg
     1021 gcgcgcagcg atcgaatcgg ggatcgactt cacactcaaa cggcaaggca gggcccatga
     1081 agtctggctg tgcggccaga cgttgctggt ggtcccgcgc catcggaacc tgaaccaact
     1141 gaccgcgcag tcgatccgca agaaactgga gagcgaattc gcgaaggact ggtggaaatg
     1201 agcgaacacc gataccgcgt cgtcgccgag cgctccggag actggtgggc gatctcagtc
     1261 cccgaccttc ctggcgtatt ttcccaggcc cggcgcctcg accaggtcga cgcgatggct
     1321 cgcgaagcca tcgccctgat gcttgacgtc gatgaaacgg cggtagcggt caccatcgac
     1381 cccacactgc cgccccgaac cgccgcggca gtgtccgagg cgctgtcccg ccggcaggca
     1441 cgcagggacg ccgaggcccg tgagcaggag gcgcttcgcc aagccgcagg cgcactcgcc
     1501 gcagacggtt tcacacaacg ggaa
//
LOCUS       DASWDC010000016         7915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1005272, whole genome shotgun sequence.
ACCESSION   DASWDC010000016 DASWDC010000000
VERSION     DASWDC010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005272"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VF995_00515"
     CDS             complement(<1..606)
                     /locus_tag="VF995_00515"
                     /inference="COORDINATES: protein motif:HMM:NF039285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342081.1"
                     /translation="MDSDTGSEHPDGRVEPAAADAATGARPGTAGGGLTDEQQRAQRL
                     AAQFFGLSILAGIALFVVYFTGGQTQLEGVLFFVALSGLGLGVGLWGIHLLAAHEAAE
                     ERHELSSGPEMRQAFEEALGEEVGPEIKRRGLLLGLLAGAGGALGLALLLPVISLGPA
                     PGNSLKVTGWRKGLRAVDENGTVLDLNAVPEDGFVTVYPEGQ"
     gene            complement(694..1491)
                     /locus_tag="VF995_00520"
     CDS             complement(694..1491)
                     /locus_tag="VF995_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9342082.1"
                     /translation="MRRTLLLLAGIGILAVLLIPATGGASTGVAAQTAGGDTGELIAR
                     GKALYLQACASCHGPDPNGASQYPSTVPSLKDVGGAAAVDWAVRTGRMPWKSTVGPAI
                     ARGRSRFDAEDTQALDAYVGSAVGDAQLPQVDPAQGDIARGRQAYAQACSACHGMNGA
                     GSALGGANIAPTLLDVKPLDVAEAIKIGPGQMPPGGGIPSYTPDDAQSIATVNDIAAY
                     VQSLQTTPYNRGGLPIGGKGPVPEGFVAWIIGLGALVLVVRWLGGRT"
     gene            1709..2536
                     /locus_tag="VF995_00525"
     CDS             1709..2536
                     /locus_tag="VF995_00525"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX9342083.1"
                     /translation="MLPSQGDTDDLTYGSYLRVGELLALQRLRSRPPHPEELHFIVVH
                     QALELWMKLLLHDLERIIALLDSGAFAEALALLGRANQIMASGLDQMRSLHTLPPWDL
                     QRFRSYLGTASGSQSQQFRELELLSGLRDPAYLKALEVEHGGALPAPLAARLQQRSLA
                     EAHADAGGRLGLDRVERWADFYQDPGDQAAFYLVCEALVDYDERWSRWRHEHVALVER
                     TLGDHARGTGGMALTYLRRTTRYRFFPLLWALRDELVVRGGGELVGRPDDRSGGEVR"
     gene            2545..3513
                     /gene="lgt"
                     /locus_tag="VF995_00530"
     CDS             2545..3513
                     /gene="lgt"
                     /locus_tag="VF995_00530"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX9342084.1"
                     /translation="MVAYIPAPPSNGFHLGPLFFHLYGLMIAIGILFAVALARRRWAA
                     RGHDPVTLERPMIWAVVAGFVGGRIGYVITHSGDFRGRWWHVIAIWEGGLALYGGLTF
                     GILTGIWVCRRRGLPVLQALDAAIPGIPLAQAFGRWGNYFNQELFGTPTKLPWGLKVD
                     PVHVPDKYVGFTTFHPTFLYESLYDLLVVGVLLWLDRRRKLRAGSIVLAYLALYAFGR
                     FFLELLRTDTTFRLLGLSRNAWISIGVVLGAGTWLFLRERRHLAEEHAETGAATGESG
                     ERPADGEAKAKEAKEEGEPVGVPAGERAAAGSGADGGDDGEGTEEG"
     gene            complement(3549..4766)
                     /locus_tag="VF995_00535"
     CDS             complement(3549..4766)
                     /locus_tag="VF995_00535"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEX9342085.1"
                     /translation="MGQFDVTSEIGALRKVVLHEPDLELARLTPSNVDELLFDEIMWV
                     ERAKQEHRAFAAVLRARGAEVFLLGELMVEALDEEAARKELLDRVVTVDAFGKGLAPE
                     LRHWFDSLPAPELVRYLIGGVIPEDLPFTPDGLVGASFHPTTFVLPPLPNQLFVRDTS
                     AWMYGGVSINPMAFPARRRETLHLETVYRYHPMFATGGFPVWYGGDGRDHYPATVEGG
                     DLLVIGNRSLLVGMGERTTHQGVEALARGMFDAGVLDTVIAVELPKVRAYMHLDTVLT
                     QVDHDAFVVYPGLRQRMRPFVVTPGSGSHLRVEAADELFATLARVLGVPRIRAFETGG
                     NELAAAREQWDDGNNVLALSPGVVLAYERNIDTNQRLRDAGVEVLTIPGFELGRGRGG
                     PRCMSCPLARDPA"
     gene            4909..5412
                     /locus_tag="VF995_00540"
     CDS             4909..5412
                     /locus_tag="VF995_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342086.1"
                     /translation="MARYLVVAHQTAGSPELIERVRSLATGDLEAAFVLLVPATPVHH
                     LLHTWEEVEARQLAWQRGQEAAKTLTEAGAKIDAVRVGAPYPLDAVADELRDHDDYAA
                     IVLSTFPPGLSRWLKGNLPGQLHRRHRLQVIHVVASSAAQAAAGTAGTAGTAGTAGTP
                     GGAPTAP"
     gene            complement(5481..5933)
                     /locus_tag="VF995_00545"
     CDS             complement(5481..5933)
                     /locus_tag="VF995_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342087.1"
                     /translation="MSSPASTGQADGQPPAAPRPRRQILGPTGLIVMVAAILAFTAVS
                     IVIELVHNYGRVYTATVQVVGQVPDRPNEFQVVFHITNRGSKPGTPDICDATLTDISG
                     ERVGTASVTLKGKNLTIAAGQTLDEPAIGTAASAPVGGDVNCHNLEPE"
     gene            complement(5991..6527)
                     /locus_tag="VF995_00550"
     CDS             complement(5991..6527)
                     /locus_tag="VF995_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342088.1"
                     /translation="MRAVSKAFAKSSRSSRPSRSWDRLKLDWEIGLHRLPAFTQRAAV
                     LLLGVVIAAFVLIGLLVSVQRTLPQPYLHLSPSEGEPPNGAAPAGGQAGAGAGAGAAT
                     TSTALPELLPSTTASSTPAAAPPSTNPQARPGATVGPTTTSRRGTTTSSTPSSTTTST
                     GWPPVTVTLPLLQRALGG"
     gene            6780..>7915
                     /gene="leuC"
                     /locus_tag="VF995_00555"
     CDS             6780..>7915
                     /gene="leuC"
                     /locus_tag="VF995_00555"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX9342089.1"
                     /translation="MANPRTLFAKLWDRHVVVEAPGLPSMLYIDLHLVHEVTSPQAFA
                     GLKARQLPVRRPDLTVATADHSTPTHDRSLPIADDLAAAQVRMLEKNCAEHGIPLHGW
                     GSPHQGIVHVIGPELGLTQPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     LQRRPRTYEVRVEGRLREGVAAKDVILALLAKIGVGGGTGHVFEYRGPAVRALSMEQR
                     MTICNMSIEGGARAGMVAPDDTTFEYVEGRPYAPKGAAWDAAVADWRTLPSDEGAVYD
                     KSVTLDANALEPMITYGTNPGMGMPITGRTPDPDHAADPATRQALAKSLAYMDLRPGE
                     PLLGQALDVVFIGSCTNSRISDLRQAAAMLKGRKVAPNVRVLVVP"
BASE COUNT         1057 a         2790 c         2914 g         1154 t
ORIGIN      
        1 ctggccctcc gggtagacgg tgacgaagcc gtcttcgggg acggcgttga ggtcgagcac
       61 ggtgccgttc tcgtcgaccg cccgcaagcc cttgcgccag ccggtcacct tgaggctgtt
      121 gccgggggct gggccgagtg agatcaccgg cagcagcagc gccaggccga gcgcgccgcc
      181 ggcgccggcg agcaggccga gcagcaggcc gcggcgtttg atctcagggc cgacctcctc
      241 cccgagggcc tcctcgaacg cctggcgcat ctcgggcccg gaggacagct cgtggcgttc
      301 ctcggccgcc tcgtgggcgg cgagcaggtg gatgccccac aggccgacgc cgaggccgag
      361 gccgctcagg gcgacgaaga agagcacgcc ctcaagctgg gtctggccgc cggtgaagta
      421 gacgacgaac agggcgatgc cggcgagtat cgacaggccg aagaactgcg cggcgaggcg
      481 ctgcgcgcgc tgctgctcgt cggtcaaccc cccgcccgcg gttccaggcc gggcgccggt
      541 cgcggcatcg gctgccgccg gctcgacccg cccgtccggg tgctcgctgc cagtgtcgct
      601 gtccacgtcg cttggctctc cttgctgctt gccgtgccgg tcgttgtcgt tgtcgttggc
      661 gttgtcgcgg gtggtggtct cgggtgggtg gtctcaggtg cggccgccga gccagcgcac
      721 cactagcacc agcgcgccga ggccgatgat ccaggccacg aagccctccg ggaccgggcc
      781 cttgccgccg atcggcaggc cgccgcggtt gtagggggtc gtctgcaggc tctgcacgta
      841 ggcggcgatg tcgttgacgg tggcgatgct ctgcgcgtcg tcgggggtgt agctggggat
      901 gccgccgcct gggggcatct ggccggggcc gatcttgatc gcctcggcca cgtcgagcgg
      961 cttgacgtcg agcagggtcg gggcgatgtt ggcgccgccg agcgcggacc cggcgccgtt
     1021 catgccgtgg caggctgagc aggcctgcgc gtaggcctgc cggccgcggg cgatgtcgcc
     1081 ctgggccggg tcgacctggg gaagctgggc gtcgcccacg gcgctgccga cgtaggcgtc
     1141 gagcgcctgg gtgtcctcgg cgtcgaagcg cgacctgccc cgtgcgatcg cagggccgac
     1201 ggtggatttc cacggcatcc gcccggtgcg gaccgcccag tcgactgcgg cggccccgcc
     1261 gacgtccttc agcgacggga ccgtgctcgg atattgcgac gcgccgttcg ggtcgggtcc
     1321 gtggcaggag gcgcaggcct gcaggtagag ggctttgccg cgggcgatca gctcgccggt
     1381 gtcgccgccg gcggtctgcg ccgccacccc ggtgctggcc ccgccggtgg cggggatcag
     1441 cagcaccgcg aggatcccga tgcccgccaa gaggagcaac gtcctgcgca cgcgcgactc
     1501 cgttccgtaa ctgacctggt cggtgacttg gatgctctcg atgggtaccc gccggctgcg
     1561 gcaccatgct acccgtgggc cacggcccgg caaccgaaac tgaccggttg tggataacct
     1621 cgggaaccag accttgggac gcgaccctcg ggcgtgtccg agggcgtgac cggacggcga
     1681 ggcggccggc gggaaggggg acggcgcggt gcttccaagc caaggggaca cggacgatct
     1741 cacctatggc agctacctgc gggtcggtga gctgctggcg ctgcagcggc tccgttcgcg
     1801 gccgccccat cccgaggagc tgcacttcat cgtcgtccac caggcgctgg agctgtggat
     1861 gaagctgctg ctgcacgacc tggagcggat catcgcgctg ctggacagcg gcgcgttcgc
     1921 ggaggcgctg gcgctgctcg gccgtgccaa ccagatcatg gccagcggcc ttgaccagat
     1981 gcgcagcctc cacaccctgc cgccgtggga cctgcagcgg ttccgcagct accttgggac
     2041 cgcctcgggt tcgcagtcgc agcagttccg cgagcttgag ctgctgtcgg gattgcgtga
     2101 cccggcctac ctgaaggcgc tggaggtcga gcacggcggc gcgctgcccg ccccgctggc
     2161 cgcgcgcctc cagcagcggt cgctggcgga ggcgcacgcc gacgcgggcg ggcggctcgg
     2221 cctggaccgg gtcgagcgct gggccgactt ctaccaggat cccggcgacc aggctgcgtt
     2281 ctatctggtc tgcgaggcgc tggtcgacta cgacgagcgc tggtcgcgct ggcgccacga
     2341 gcatgtcgca ctggtcgagc ggaccctcgg cgaccatgcc cgcggcaccg gcgggatggc
     2401 cctcacctac ctgcggcgca ccacccgcta ccggttcttc ccgctgctgt gggcactgcg
     2461 cgacgagctg gtcgtccgtg gcggcgggga gctggtcggc cgaccggatg accgttcagg
     2521 cggcgaagtg cgatgattgc ctctatggtt gcctacattc ccgccccgcc gagcaacggg
     2581 ttccatctcg gcccgctgtt cttccatctg tacgggttga tgatcgccat cggcatcctg
     2641 ttcgcggtgg cgctggcgcg gcgccgctgg gcggcccgcg gccacgaccc ggtcaccttg
     2701 gaacggccga tgatctgggc ggtcgtcgcc ggcttcgtcg gcggccgcat cggctacgtg
     2761 atcacccaca gcggcgattt ccggggccgc tggtggcacg tgatcgccat ctgggagggc
     2821 ggcctcgccc tctacggcgg gctcaccttc gggatcctca ccgggatctg ggtgtgccgc
     2881 cgccgcggcc tgccggtgct gcaggcgctg gacgcggcga tcccggggat cccgctggcg
     2941 caggcgttcg gccgctgggg gaactacttc aaccaggagc tgttcggcac gccgaccaag
     3001 ctgccctggg ggctgaaggt cgacccggtc cacgttcccg acaagtacgt cggcttcacg
     3061 accttccatc cgacgttcct gtacgagtcg ctgtacgacc tgctggtggt cggcgtgctg
     3121 ttgtggctgg accggcgccg caagctccgg gccggctcga tcgtgctcgc ctacctggcg
     3181 ctgtacgcct tcggccgttt cttcctggag ctgctgcgca ccgataccac cttccggctg
     3241 cttggattgt cccggaacgc ctggatctcg atcggggtcg tgctcggcgc gggcacctgg
     3301 ctgttcctcc gggaacgccg ccaccttgcc gaggagcacg ccgagaccgg cgccgcgacc
     3361 ggcgagtccg gggagcggcc cgccgacggc gaggccaagg ccaaggaggc caaggaggag
     3421 ggcgagccgg tgggcgtccc cgccggcgag cgcgccgccg ctggcagtgg cgccgatggc
     3481 ggcgatgacg gcgagggcac tgaggagggc tagcgtcgcg ggtcccgcgc cctctcccca
     3541 aagggtcgtc aggcggggtc gcgggcgagc gggcagctca tgcagcgcgg gccgccgcgg
     3601 ccgcggccca gctcgaaccc ggggatggtg agcacctcca ccccggcgtc gcgcaagcgc
     3661 tggttggtgt cgatgttgcg ctcgtaggcc agcaccaccc cgggcgagag ggcgagcacg
     3721 ttgttgccgt cgtcccactg ctcgcgggcg gcggccagct cgttgccgcc ggtctcgaag
     3781 gcgcggatcc gcggcacgcc gagcacccgc gcgagcgtgg cgaacagctc gtcagcggcc
     3841 tccacccgca ggtggctgcc gctgccgggg gtgacgacga agggccgcat ccgctgccgc
     3901 aggcccgggt agaccacgaa ggcgtcgtgg tcgacctggg tgaggacggt gtcgaggtgc
     3961 atgtaggccc gcaccttcgg cagctcgacg gcgatcacgg tgtcgagtac cccggcgtcg
     4021 aacatgccgc gggcgagcgc ctcgacgcct tggtgggtgg tgcgctcacc catgccgacc
     4081 agcaggctcc ggttgccgat caccagcagg tcgccgcctt cgaccgtcgc cgggtagtgg
     4141 tcccgcccgt cgccgccgta ccagacgggg aagccgccgg tggcgaacat cgggtggtag
     4201 cggtagacgg tctccaggtg cagggtctcg cggcggcggg cggggaacgc catcgggttg
     4261 atcgagaccc ccccgtacat ccacgcggag gtgtcgcgga cgaacagctg gttgggcagc
     4321 ggcggcagca cgaaggtggt cgggtggaag ctggcgccga ccagcccgtc gggggtgaac
     4381 ggcaggtcct cggggatcac cccgccgatc aggtagcgga ccagctcggg cgcgggcagc
     4441 gagtcgaacc agtggcgcag ctccggcgcg aggcccttgc cgaaggcgtc gacggtcacc
     4501 acccggtcga gcagctcctt gcgggcggcc tcctcgtcga gcgcctcgac catcagctcg
     4561 ccgagcagga acacctccgc cccgcgggcg cgcaggaccg ccgcgaaggc gcggtgctcc
     4621 tgcttggcgc gttccaccca catgatctcg tcgaacagca gctcgtcgac gttggagggc
     4681 gtgagccgcg ccagctccag gtcaggttcg tgcagcacca ccttgcgcaa ggcgccgatc
     4741 tcggaggtga cgtcgaactg ccccataccc gcaggctacc agccgccggg cgcgtggccg
     4801 gcggcgtgaa ccgggtgctg gccggcctct tgacagcact cccctgccgg ggcgaaaacc
     4861 aaggacacaa accgcgcaag cggccctccg gccccgaggg agactgtcat ggctcgctac
     4921 ctcgtcgtcg cgcaccagac cgccgggagc cccgagctga tcgagcgggt ccggtccctg
     4981 gcaaccggcg atctcgaagc cgcgttcgtg ttgctggtgc cggccacgcc ggtccatcac
     5041 ctgctgcaca cctgggagga ggtcgaggcc aggcagctcg cctggcagcg cggccaggag
     5101 gccgccaaga cgctcaccga ggccggcgcg aagatcgacg cggtccgggt cggtgcgccc
     5161 tacccgctcg acgcggtcgc cgacgagctg cgcgaccacg acgactacgc ggcgatcgtg
     5221 ctgtcgacgt tcccgccggg tctgtcacgc tggctgaagg ggaacctgcc gggccagctg
     5281 caccggcgcc accgcctcca ggtcatccac gtggtggcgt cgtcggcggc gcaggcggca
     5341 gcgggcacgg cgggcacggc gggcacggcg gggacggcgg ggacaccggg cggcgcgcca
     5401 accgcaccct gaggctacag ggagcggctg ggcggcggcg cgacgctacc ggacgctacc
     5461 gggtcggcgc ttccggtcgg ctattccggt tcgaggttgt ggcagttgac gtcgccgccg
     5521 accggcgcgg acgcggcggt gccgatggcc ggctcgtcca gggtctggcc ggcggcgatg
     5581 gtgaggttct tgcccttgag ggtgacgctg gcggtgccga cccgctcgcc gctgatgtcg
     5641 gtcagggtcg cgtcgcagat gtcaggggtg ccgggcttgc tgccgcggtt ggtgatgtgg
     5701 aacaccacct ggaactcgtt cgggcggtcg gggacttggc cgaccacctg gacggtggcg
     5761 gtgtagacac gcccgtagtt gtgcaccagc tcgatgacga tgctgacggc ggtgaaggcg
     5821 aggatcgcgg ccaccatcac gatcaggccg gtcgggccga ggatctgacg cctgggtcgc
     5881 ggtgcggcgg gcggctggcc gtccgcctgt ccagtcgatg cggggctgct cacgccccaa
     5941 ggctaccgca cccgcggccg cttggcgcac cgcgccggac tgccgcggcc tcagccaccg
     6001 agtgcccgct ggagcagcgg cagggtgacc gtcaccggcg gccagcccgt cgaggtggtg
     6061 gtgctgctcg gcgtgctgga ggtggtggtg ccgcggcggc tggtggtggt cgggcccacg
     6121 gtggcgccgg gtcgggcctg agggttggtc gatggcggcg cggcggcggg ggtgctggac
     6181 gctgtcgttg agggaagcag ctctggcagc gccgtcgagg tcgtcgccgc acccgcgcca
     6241 gctccggcgc cggcctggcc gccggcgggc gcggccccgt tcggcggctc accctcggag
     6301 gggctgaggt gcaggtaggg ctgcgggagg gtccgctgga cgctgaccag caggccgatc
     6361 aggacgaacg cggcgatgac cacgccgagc agcagcaccg cggcccgctg cgtgaacgcg
     6421 ggcagccggt gcagtccgat ctcccagtcc agcttcagcc ggtcccacga ccgggagggc
     6481 ctggaggacc tcgacgattt tgcgaatgcc ttggagaccg cgcgcacacc agacgctttc
     6541 ccccttggac gcgaccacga caggacggtt gtgtggcgcg aaccataccc gcgatggtcg
     6601 ggaaggtcca atcgggccga ttcgcttcgc ctgctcggtc tgcttgggtt atccacaact
     6661 cagcggttcg gttcgccgca ccgctcggct tgcccgtagg atgggcgcca acgccagcgg
     6721 cgattgccaa cccgatcgcc aacggcgatg tcggcttttc ggatcagcag aggaggacca
     6781 tggccaaccc gcgtaccctg ttcgcgaaac tctgggaccg ccatgtggtg gtcgaggcac
     6841 ccggccttcc gagcatgctc tacatcgacc tccacctcgt ccacgaggtg acctcgccgc
     6901 aggcgttcgc gggcctcaag gcgcgccagc tccccgtccg ccgcccggac ctgacggtgg
     6961 ccaccgccga ccactccacc ccgacccacg accgctccct gccgatcgcc gacgacctcg
     7021 ccgccgcgca ggtgcggatg ctggagaaga actgcgccga gcacggcatc ccgctgcatg
     7081 gctggggcag cccccaccag ggcatcgtcc atgtgatcgg cccggagctg ggcctcaccc
     7141 agccgggcat gacgatcgtc tgcggcgact cccacaccgc cacccacggc gccttcggcg
     7201 cgctcgcctt cggcatcggc accagcgagg tcgagcacgt gctcgccacc cagacgctgc
     7261 tgcagcgtcg ccccaggacc tatgaggtcc gcgtcgaggg ccgcctgcgc gagggggtcg
     7321 ccgccaagga cgtgatcctg gcgctgctcg ccaagatcgg cgtcggcggc ggcaccggcc
     7381 acgtcttcga gtaccgcggc ccggcggtcc gcgcgctgtc gatggagcag cgcatgacca
     7441 tctgcaacat gtcgatcgag ggtggcgccc gcgccggcat ggtcgccccc gacgacacca
     7501 ccttcgaata cgtcgaaggc cgcccctacg cccccaaggg cgccgcctgg gacgcggccg
     7561 tggccgactg gcggaccctg ccaagcgacg agggcgccgt ctacgacaag tcggtgacgc
     7621 tggacgccaa cgccttggag ccgatgatca cctacggcac caaccccggc atgggcatgc
     7681 cgatcaccgg ccgcaccccc gacccggacc acgccgccga cccggcgacc cggcaggcac
     7741 tggccaagtc gctcgcctac atggacctgc gccccggcga gccgctgctc ggccaggccc
     7801 tcgacgtggt gttcatcggc tcctgcacca actcccgcat ctccgacctg cgccaggccg
     7861 ctgccatgct gaagggccgc aaggtcgccc cgaacgtgcg ggtgctggtc gtgcc
//
LOCUS       DASWDC010000017         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1589821, whole genome shotgun sequence.
ACCESSION   DASWDC010000017 DASWDC010000000
VERSION     DASWDC010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589821"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..85)
                     /locus_tag="VF995_00560"
     CDS             complement(<1..85)
                     /locus_tag="VF995_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX9342090.1"
                     /translation="MLLLPCPWCGPRDEAEFRYGGQAGIAYP"
     gene            complement(102..1307)
                     /locus_tag="VF995_00565"
     CDS             complement(102..1307)
                     /locus_tag="VF995_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEX9342091.1"
                     /translation="MATRYWRAAEPRRAYDVVVVGGGGHGLATAYYLARNHHVTDVCV
                     VEKGWLGGGNTARNTTIIRSNYLWDESAAIYEHALRLWEGLEEDLGLDLRFSQRGVLN
                     LAHDLGEVRSSVRRVNANALNGVDAEWLTPEEVAAFCPIVNLSPELRYPVLGATLQRR
                     GGIARHDRVAWGFATAADRLGVDIVEHCEVTGFVRAPGPGGGVAGVETTRGTITANKV
                     AVVAAGHTSVLAAMVGLRLPLQSHPLQALVSVLLEPVLDCVVMSNAVHVYVSQADKGE
                     LVMGAGIDGHNGYSQRGAFHLIEEQLAAALELFPVFTHAKLLRTWGGIVDVCPDASPI
                     IGLTPIEGLYLNCGWGTGGFKATPGSGWVFAETIAKGEPAPLALPFALDRFTTGALID
                     EHGAAAVAH"
     gene            1360..2136
                     /locus_tag="VF995_00570"
     CDS             1360..2136
                     /locus_tag="VF995_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9342092.1"
                     /translation="MSDEVRGGVQSVTRALQALELIAEAGELGVTDLGRRLGVHKATA
                     SRLAATLAGGGLVERDPVSDRYRLGFGLIRLAGTAMASLDLVRTSRPVLEDLAERTRE
                     SVTLGVFSNDEVIYVDQVSGPSLIVSGNWIGRRTPLHCSASGKVFLAWLAPDKRDQLL
                     AGPLEVITPRTVTDLASLHQQLVEARIRGYATLQDELEEGLNAVAAPVRRASGEVMAA
                     IAVSGPSFRVRPVDLPRLGRVTVDAAMAVSRRLGHAVRPG"
     gene            2209..>2499
                     /locus_tag="VF995_00575"
     CDS             2209..>2499
                     /locus_tag="VF995_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9342093.1"
                     /translation="MDPQTHDRQLIRELVENWALWRDAGDWERFRGVWHDDGRMMATW
                     FQGPFEEFITVSREGFERGVSILHFLGGHTAELATDRAIAQTKMHIMQRGPVH"
BASE COUNT          367 a          931 c          869 g          332 t
ORIGIN      
        1 ccgggtaggc gatcccggcc tggccgccgt agcggaactc agcctcgtca cgcggcccac
       61 accaggggca ggggagcagc agcatcagtg cgccaccgtc atcagtgcgc caccgccgcc
      121 gcgccgtgct cgtcgatcag cgccccagtg gtgaagcggt cgagggcgaa cgggagcgcc
      181 agcggcgccg gctcgccctt ggcgatcgtc tcggcgaaca cccagccgga gcccggggtc
      241 gccttgaagc cgccggtgcc ccagccgcag ttcaggtaga ggccctcgat cggggtcaga
      301 ccgatgatcg gggaggcgtc ggggcagacg tccacgatcc cgccccaggt ccgcagcagc
      361 ttggcgtggg tgaacaccgg gaacagctcc agcgcggcgg cgagctgctc ctcgatcaga
      421 tggaaggcgc cccgctggct gtagccgttg tggccgtcga tgccggcgcc catcaccagc
      481 tcgcccttgt cggcctgcga gacgtagacg tggaccgcgt tggacatcac cacgcagtcc
      541 agcaccggct ccagcagcac cgacaccagc gcctgcagcg ggtgcgactg caggggcagc
      601 cgcaggccca ccatcgccgc cagcaccgag gtgtggccgg cggcgaccac cgcgaccttg
      661 ttggcggtga tcgtccccct ggtggtctcg accccggcaa cccctccacc cggtcccggt
      721 gcgcggacga acccggtcac ctcgcagtgc tccacgatgt ccacgccgag ccggtcggcg
      781 gcggtcgcga agccccaggc gacccggtcg tggcgagcga tcccgccgcg ccgctgcagg
      841 gtcgcgccga gcaccgggta gcggagctcc ggggagaggt tgacgatcgg gcagaacgcg
      901 gccacctcct cgggggtgag ccactcggcg tcgacgccgt tgagggcgtt ggcgttgacc
      961 cgccgaaccg aggagcgcac ctcgccgagg tcgtgggcca ggttcagcac cccgcgctgg
     1021 ctgaagcgca ggtccagccc gaggtcctcc tccagcccct cccagagcct cagcgcgtgt
     1081 tcgtagatcg ccgcggattc gtcccacagg tagtttgacc ggatgatggt ggtattgcgg
     1141 gcggtgttgc cgccgccgag ccagcccttc tcgaccacgc agacgtcggt gacgtggtgg
     1201 ttgcgcgcca ggtagtaggc ggtcgccagg ccgtggccgc cgccgccaac cacgacgacg
     1261 tcgtaggcgc gcctgggctc ggccgcccgc cagtaccgcg tggccatacc agcaagccta
     1321 cctgggacag gactgccgat cgcaggctag agttgcgcta tgagcgacga agtacgtggc
     1381 ggcgtacagt cggtcactcg cgcattgcag gcgcttgaat tgatcgcgga ggccggcgag
     1441 ctgggcgtga ccgacctcgg ccgccgcctc ggcgtccaca aggcgaccgc ctcgcgcctg
     1501 gcggccaccc tcgccggcgg cggcctggtc gagcgcgacc cggtgagcga ccgctaccgg
     1561 ctcgggttcg ggctgatccg cctcgccggc accgccatgg ccagcctcga cctggtccgc
     1621 accagccgcc ccgtgctgga ggacctcgcc gagcggacgc gggagagcgt caccctcggg
     1681 gtcttctcca acgacgaggt catctacgtc gaccaggtct cgggcccgag cctgatcgtc
     1741 agcggcaact ggatcggccg gcgcaccccc ctgcactgca gcgccagcgg caaggtgttc
     1801 ctggcctggc tcgccccgga caaacgggac cagctgctcg ccgggccgct ggaggtgatc
     1861 accccgagga ccgtgaccga cctcgccagc ctccaccagc agctggtgga ggcccgcatc
     1921 cgcggctacg ccaccctcca ggacgagctc gaggagggcc tcaacgccgt cgccgccccc
     1981 gtccgccggg ccagcggcga ggtgatggcc gccatcgcgg tctcgggtcc ctccttccgc
     2041 gtccgcccgg tcgacctgcc ccgcctcggg cgggtcaccg tcgacgccgc catggcggtg
     2101 tcgcgccgcc tcggccacgc cgtccggccg ggatagcgat ccgctcggcc tcccagagcc
     2161 cccggaacaa cgctgacact ggaggtgacc gtgagcacgg acctggacat ggacccgcag
     2221 acgcacgacc gccagctcat ccgcgagctg gtcgagaact gggcgctgtg gcgcgacgcc
     2281 ggcgactggg agcggttccg cggcgtctgg cacgacgacg gccgcatgat ggccacctgg
     2341 ttccagggcc ccttcgagga gttcatcacg gtcagccgcg aggggttcga gcgcggggtg
     2401 agcatcctgc acttcctcgg cggccacacc gccgagctgg ccaccgaccg ggcgatcgcg
     2461 cagaccaaga tgcacatcat gcagcgcggc cccgttcac
//
LOCUS       DASWDC010000018         5463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1590485, whole genome shotgun sequence.
ACCESSION   DASWDC010000018 DASWDC010000000
VERSION     DASWDC010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590485"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VF995_00580"
     CDS             <1..574
                     /locus_tag="VF995_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX9342094.1"
                     /translation="LGARTSPFARNWTVHDYAAIAPLVDRLRIMTYDYHSPGSTPGPV
                     APLWWVEAVASYALTVAPAGKVELGVPLYGYDWPADSRRWSGWGTASSLTYLKAEALR
                     AHYHAKRQWSSVDAAPWFTYTTRAGVRHVVWYNDAASTRAKLGLVVNYHLRGLAFWVV
                     GSEDARQWPAIRAFTGPPAAAHPKRPARGH"
     gene            675..1409
                     /locus_tag="VF995_00585"
     CDS             675..1409
                     /locus_tag="VF995_00585"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX9342095.1"
                     /translation="MSVFQPDAASGPRIASPQPAFKVARRGYDPQEVDAYVAELIARL
                     EETGARADLAERSKAKIESEITSLKEQPPTFERLGVDMAEVLEQAGRSAELLVEKAQT
                     RAGTIVKEAEAHAAELVASAGAKAAELDATARQRLADVRAETEQVREFRDGLAHHLGR
                     VRADIDALLQRLDEQGAALGPVAAPAPAAAPAPAQSDAKVEGEAAAAPEAAAATPQSG
                     NTAEARPAPHNGGRPPVERRPPNQRG"
     gene            complement(1432..2658)
                     /locus_tag="VF995_00590"
     CDS             complement(1432..2658)
                     /locus_tag="VF995_00590"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018841906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX9342096.1"
                     /translation="MDEPVLARARALLRAHPVVDGHNDLAWALREKARYDLDRVDVAK
                     PQPELHTDIPRLRAGGVGAQFWSVYVPSKLQGDQAVSATLEQIDAVHRLVARYPDTFA
                     LAGDADEVERARSAGRIASLLGAEGGHSIDCSLGTLRMLYALGVRYLTLTHNDNVPWA
                     DAATDQPRAGGLTRFGEEVVRELNRLGMLVDLSHVAATTMRAALAVSQAPVIFSHSSA
                     RALCDVPRNVPDNVLSILAANGGVCMVTFVPMFVSPACAAWFEEGRAHVAGLGTDPDD
                     CGASMPLIRAWTAGHPPPRVTVTEVADHVEHVRAVAGIDHVGLGGDYDGVDFQPEGLQ
                     DVSGYPNLVAELLERGWSDPEIAKLTWGNALRVLREAEAVARTLRQRRGPSLATWADL
                     DGADGTEAGGSEVAPG"
     gene            2834..4066
                     /locus_tag="VF995_00595"
     CDS             2834..4066
                     /locus_tag="VF995_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9342097.1"
                     /translation="MALVLEGGRLIDGSGRDPVERSRVVVEGDRIAAVGAGGDHPRGA
                     EVVGLDGLTVLPGLIDLHTHMGIVTIGELAGLPLAVVAAQLFRNAELCLLSGHTTVRE
                     VGGADGGLRQAIDQGLLPGPRLFPSGPLLSQSGGHGDLAPPFLALEHRHQGGVPGLVQ
                     PHATCDGPDEVRVAARTAFRHGATQIKMCVSGGVVSLTDRVEDAQFTVEELRAAVEEA
                     QARDSYVTAHAHNVRGILNGLEAGVACFEHGTFLDEPTAAKMAVARAALVPTLAVTQI
                     FVEQAEAWGIPPSIVPRMAGVGEAMANAVKIAADAGVMVGSGTDILGPEQNRRGLELV
                     LKAQILGPMAAIVSATATNARILRRETDLGTVEEGKLADLIAIDGDPLADPGVFDDPH
                     RVVLVVKDGQVVKDTRSR"
     gene            4299..5417
                     /locus_tag="VF995_00600"
     CDS             4299..5417
                     /locus_tag="VF995_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HEX9342098.1"
                     /translation="MELAAAVEFLRQYHAEEPSAGPLGPRLRAVRADVERYGTYWHTP
                     GELAFGARVAWRNASRCIGRLYWRGLRVRDRRSATGADEIAAECVTHLREATNGGQIR
                     PTITVFSPTLPWRPTPRIWNEQLIRYAGHRTGERGQVVGDPRNAGFTDLVHRLGWRGG
                     SGGPFDVLPLVVKVHGERPRLYDLPADAVLQVPLTHPRHAWFAELGLRWCALPALSNM
                     CLEIGGVCYPAAPFNGWYMGTEIGARNLADLDRYNQLPVIAQRLGLDTSRDRTLWRDL
                     ALVELNIAVLHSFEAAGVTMTDHHTESRRFLTHLAREEQAGRPCPADWSWIAPPISGS
                     ATPVFHRYYDEAALLPTYTLHPDASARSRGECPGHMPM"
BASE COUNT          761 a         2046 c         1945 g          711 t
ORIGIN      
        1 gctcggggcg cggaccagcc ccttcgcccg caactggacg gtccacgact acgcggcgat
       61 cgcgccgctg gtggaccgcc tgcggatcat gacctacgac taccacagcc ccggcagcac
      121 ccccggcccg gtcgcgccgc tgtggtgggt ggaggccgtg gccagctacg ccctgacggt
      181 cgccccggcc ggcaaggtcg agcttggcgt gcccctgtac ggctacgact ggcccgccga
      241 ctcgcgccgc tggagcggct ggggcaccgc cagctcgctg acctacctga aggccgaggc
      301 gctgcgggcg cactaccacg ccaagcggca gtggtcgagc gtcgacgcgg cgccctggtt
      361 cacctacacc accagggccg gggtgcggca cgtcgtctgg tacaacgacg ccgcctcgac
      421 cagggcgaag ctcggcctgg tcgtcaacta tcatctgcgc gggctggcgt tctgggtggt
      481 cggctccgag gacgcccgcc agtggcccgc gatccgcgcc ttcaccggac cgcccgccgc
      541 cgcgcacccg aagcgtcccg cacgcggcca ctgagctgcc gcttcccaaa gcgacggctc
      601 gatttgctag ttgtgcgttc aaggtgcagg cttccgcaat ccagcagctc gccgggttgt
      661 acgaaggagg ggccatgagc gtgtttcagc cggacgcggc gtccgggcca cgcatcgcaa
      721 gtccgcagcc ggccttcaag gtggcacggc gggggtatga cccccaggag gtcgacgcct
      781 acgtcgcgga gctgatcgcg cgcctggagg agaccggcgc gcgggccgac ctggccgaac
      841 ggtccaaggc caagatcgag tcggagatca cctccctcaa ggaacagccg cccaccttcg
      901 agcggctcgg cgtggacatg gccgaggtgc tggagcaggc gggacgctcc gccgagctgc
      961 tggtcgagaa ggcccagacc cgcgcgggca ccatcgtcaa ggaggcggag gcgcacgccg
     1021 ccgagctggt cgcctccgcc ggcgcgaagg cggccgagct cgacgcgacc gcacgccagc
     1081 gcctcgccga cgtgcgtgcc gagaccgagc aggtgcggga gttccgcgac gggctggccc
     1141 accaccttgg ccgcgtccgc gccgacatcg acgcgctgct gcagcgcctc gacgagcagg
     1201 gcgccgcgct cggcccggtg gccgctcccg ctcccgccgc ggcgccggcg ccggcgcaaa
     1261 gcgacgccaa ggtcgagggc gaggccgcgg cggcgcccga ggccgcagcc gccacgccgc
     1321 agtcgggcaa caccgcggag gcacggccgg cgccgcacaa cggcggccgt cccccagtgg
     1381 agcgccggcc acccaaccag cgcggctagc gagccgggcg ctacccgggc gctacccggg
     1441 cgctacctcg ctcccgcccg cttcggtgcc gtcggcgccg tcgaggtccg cccaggtcgc
     1501 cagggagggg ccgcggcgct gccgcagggt gcgggcgacc gcctcggcct cgcggaggac
     1561 ccgcagggcg ttgccccagg tcagtttggc gatctcggga tcggaccagc cgcgttccag
     1621 cagctcggcc acgaggttcg ggtagccgct gacgtcctgg aggccctccg gctggaagtc
     1681 cacgccgtcg tagtcgccgc cgaggccgac gtggtcgatc ccggcgaccg cgcggacgtg
     1741 ctcgacgtgg tcggcgacct cggtcacggt gacgcgcggc gggggatggc cggccgtcca
     1801 cgccctgatc aagggcatgg aggcgccgca gtcgtcgggg tcggtgccga ggccggcgac
     1861 gtgcgccctg ccctcctcga accaggccgc gcaggcgggc gagacgaaca tcggcacgaa
     1921 ggtcaccatg cagaccccgc cgttggcggc gaggatcgag agcacgttgt cgggcacgtt
     1981 gcggggcacg tcgcacaggg cccgggcgga ggagtgggag aagatcaccg gcgcctggct
     2041 caccgccagc gccgcgcgca tcgtcgtcgc cgcgacgtgg ctcaggtcga ccagcatgcc
     2101 gaggcggttc agttcccgca cgacctcctc gccgaagcgg gtgaggccgc cggcgcgggg
     2161 ctggtcggtg gcggcgtcgg cccagggcac gttgtcgttg tgggtcagcg tcaggtaccg
     2221 caccccgagc gcgtacagca tccgcagggt cccaagcgaa cagtcgatcg agtggccacc
     2281 ctcggcgccg agcagcgaag cgatccgccc cgccgaccgc gcccgctcga cctcgtcagc
     2341 gtcaccggcc agcgcgaagg tgtcggggta gcgggccacc agccggtgga ccgcgtcgat
     2401 ctgctccagg gtggcgctga ccgcctggtc gccctgcagc ttggagggga cgtagaccga
     2461 ccagaactgg gcgccgaccc cgccggcccg cagcctcggg atgtcggtgt gcagctccgg
     2521 ctggggcttg gcgacgtcga cgcggtccag gtcgtagcgg gccttctcac gcagcgccca
     2581 cgccaggtcg ttgtggccat ccaccaccgg gtgggcacgc agaagggcgc gcgcccgcgc
     2641 gagcaccggt tcgtccatgg cccgactcta ccggtccacg cggcgggcgc gctcctggcc
     2701 ccggcctcag tgcgcacgcg ggtggggtca agcgccggag cgcggtagat cgggcggcgc
     2761 cgccgggcgt agcgttggcg cggtcgcagc tcattggcgg ctcgtgcagt tccgggactc
     2821 aggaggcgga cagatggcgc tggtcctcga aggcggcagg ctgatcgacg ggagcggccg
     2881 ggacccggtc gagcgctcgc gggtagtggt cgaaggcgat cgcatcgccg cggtcggcgc
     2941 gggcggcgac cacccgcgcg gcgcggaggt ggtcgggctg gacggcctga ccgtgctgcc
     3001 cggcctgatc gacctgcaca cccacatggg gatcgtcacc atcggcgagc tggccggcct
     3061 gccgctggcg gtggtcgccg cgcagctgtt ccgcaacgcc gagctgtgcc tgctgtccgg
     3121 ccacaccacc gtgcgggagg tcggcggagc ggacggcggc ctccgccagg cgatcgatca
     3181 gggcctgctg cccgggccgc ggctgttccc ctccgggccg ctgctgtcgc agtcgggcgg
     3241 ccacggcgac ctggcgccgc cgttcctggc actggagcat cgccaccagg gcggcgtccc
     3301 cggtctggtg cagccgcacg ccacctgcga cgggcccgac gaggtccgtg tcgccgcccg
     3361 caccgccttc cgtcatggcg ccacgcagat caagatgtgc gtctccggcg gggtggtgtc
     3421 gctcaccgac cgtgtggagg acgcccagtt caccgtcgag gagctgcgtg cggcggtcga
     3481 ggaggcgcag gcccgcgaca gctatgtcac cgcccacgcc cacaacgtgc gcggcatcct
     3541 caacggcctg gaggccgggg tggcgtgctt tgagcacggc accttcctgg acgagccgac
     3601 cgcggccaag atggcggtgg ctcgggcggc attggtcccc accctggcgg tcacccagat
     3661 cttcgtcgag caggcggagg cttgggggat ccccccgtcg atcgtgccgc gcatggccgg
     3721 ggtcggcgag gcgatggcca acgcggtcaa gatcgccgcc gacgccggcg tcatggtcgg
     3781 gtccggcacc gacattctcg ggcccgagca gaaccgccgc gggctggagc tggtcctcaa
     3841 ggcgcagatc ctcggcccga tggcggcgat tgtctccgcc accgcgacca acgcgaggat
     3901 cctgcggcgc gagaccgacc tcggcaccgt cgaggagggc aagctcgccg acctgatcgc
     3961 catcgacggc gacccgctgg ccgacccggg cgtgttcgac gacccgcatc gggtggtgct
     4021 ggtggtcaag gacgggcagg tggtcaagga cacccgcagc cgctaaggtg ctggctttag
     4081 cgactcgcga cgaacacccg gagcacccgg actgccagga acaaacaaac acgctagctt
     4141 cgttaccctt gcgcttgaat gcgcactatg gcagagtgtc gccttgtgtg acaagcgggc
     4201 atattcggaa ttggggggcg cgtgtgttgg ccaaggccac tcctgtcagc gttcagcgga
     4261 gcaaccaggc gcagggtcgg acggcggcgg gtggccgggt ggagctggcg gcagccgtgg
     4321 agttcctgcg ccagtaccac gccgaggagc cgtcggccgg gccgctcggg ccccgcctgc
     4381 gggccgttcg cgccgacgtc gagcgctacg gcacctactg gcacaccccc ggcgagctgg
     4441 ccttcggcgc ccgggtcgcc tggcgcaacg ccagccgctg catcggccgg ctctactggc
     4501 gcggtctgcg cgtccgtgac cgccgcagcg ccaccggcgc cgacgagatc gccgccgaat
     4561 gcgtgaccca cctgcgcgag gccaccaacg gcggccagat ccgcccgacc atcaccgtgt
     4621 tctcgccgac gctgccctgg cggccgaccc cgcggatctg gaacgagcag ttgattcgct
     4681 acgccggcca ccgcaccggc gagcgcggtc aggtggtggg ggacccgcgc aacgccggct
     4741 tcaccgatct ggtccaccgc ctcggctggc gcggcggcag cggcggcccc ttcgacgtgc
     4801 tccccctggt cgtcaaggtc cacggcgagc gcccccgcct ctacgacctc cccgccgacg
     4861 cggtgctcca ggttcccctc acccatcccc gtcacgcctg gttcgccgag ctgggcctgc
     4921 gctggtgcgc cctcccggcc ctgtcgaaca tgtgcctgga gatcggcggc gtctgctacc
     4981 cggccgcccc cttcaacggc tggtacatgg gcaccgagat cggcgcccgc aacctcgccg
     5041 acctcgaccg ctacaaccaa ctcccggtga tcgcgcaacg cctcggcctc gacacctccc
     5101 gcgaccgcac cctctggcgc gacctcgccc tggtggagct caacatcgcc gtcctccact
     5161 ccttcgaagc cgcgggcgtc accatgaccg accaccacac cgaatcccgc cgcttcctca
     5221 cccacctcgc ccgcgaagaa caggccggcc gcccctgccc cgccgactgg agctggatcg
     5281 ccccccccat cagcggctcc gccacccccg tcttccaccg ctactacgac gaagccgccc
     5341 tcctccccac ctacaccctc caccccgacg cctccgcccg ctcccgcggc gaatgcccag
     5401 gacatatgcc gatgtgaggg gattacagca gtgtcaggcc gtgaactgcg aggggctcga
     5461 agt
//
LOCUS       DASWDC010000019         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1590755, whole genome shotgun sequence.
ACCESSION   DASWDC010000019 DASWDC010000000
VERSION     DASWDC010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590755"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..>1644)
                     /locus_tag="VF995_00605"
     CDS             complement(160..>1644)
                     /locus_tag="VF995_00605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9342099.1"
                     /translation="APPAADEGRLAAGAPLVAAAGRALDEASFALTPDGATILTTWAP
                     HDDVRNPRTQLAALATASGERRVLFDDVASSVDAVACSPEGRMVVCQRAWHGTPDESP
                     DVTLWLVDLASGQGRDLLPGFGLWPEQPVWAPDGSAVFFVADEAGRTPVFRVEVLSDG
                     AGDPDGPARVTRLSADGCFSDLCPAPDGRRLFALRSTVGAPPGVVLLGTIATDQEPAP
                     LPTPGLPVAVPGRVTEVHAAADDGTTLRGWLILPPEASAGTPAPLVTVIHGGPLGSWS
                     GWHWRWNPHLLAARGYACLLPDPALSTGYGQAFIARGRGRWGQEPYRDVLALVDAAET
                     RPDIDKTRTAAMGGSFGGYLANWIAGHTTRFRCVVTHASLWSLDQFHGTTDDAGWWDR
                     EFGPPEEAAPRYRENSPSRHADAVQVPMLVIHGERDYRVPISEALRLWTDLAWRGLDA
                     KFLYFPDENHWILKPQHARVWYETVLAFLDHHVLGKDWSRPELL"
BASE COUNT          227 a          586 c          622 g          209 t
ORIGIN      
        1 gcaagaacct gctcgacctg cgccgagtcg ccgtggtcca caacctccac gttatcgccc
       61 gccaaccccg accagcccag caagccgcct gaaccggata cccgtccggc tctctaggaa
      121 aggtgtcgag ggtggtccgg ggtgtctggg gcagctcgtc tagagcagct cggggcggga
      181 ccagtctttg ccgaggacgt ggtggtcgag gaaggcgagg acggtctcgt accagacgcg
      241 ggcgtgctgg ggtttgagga tccagtggtt ctcgtcgggg aagtagagga acttcgcgtc
      301 caggccacgc caggcgaggt cggtccacag tcggagggcc tcgctgatgg ggacgcggta
      361 gtcccgctcg ccgtggatga ccagcattgg cacctggacg gcgtcggcgt ggcggctggg
      421 ggagttctcg cggtagcggg gggctgcctc ttctgggggg ccgaactcgc ggtcccacca
      481 gccggcgtcg tcggtggtgc cgtggaactg gtcgagcgac cacaggctgg cgtgggtgac
      541 cacgcagcgg aagcgggtgg tgtggcccgc gatccagttg gccaggtagc cgccgaagga
      601 gccgcccatc gcggcggtgc gggtcttgtc gatgtcgggg cgggtctcgg ccgcgtcgac
      661 cagcgccagc acgtctcggt acggctcctg cccccagcgg ccccggccgc gtgcgatgaa
      721 cgcctgcccg tagccggtcg acagcgccgg gtcgggcagc aggcaggcat agccccgcgc
      781 cgccagcaga tgcgggttcc agcgccagtg ccagcctgac cacgagccga gcgggccgcc
      841 atggatgacc gtcaccagcg gcgccggggt tcccgccgat gcctccggtg gcagtatcag
      901 ccagccgcgc agcgttgttc cgtcgtcggc ggcggcgtgc acctcggtga cccgccctgg
      961 caccgccacc ggcaggccgg gggtgggcag cggggccggc tcctggtcgg tggcgatggt
     1021 gccgagcagc accaccccgg gcggcgcgcc gaccgtcgag cgcagcgcga acagccgccg
     1081 cccatccggc gcgggacaca ggtcggagaa gcatccgtcc gcgctcaacc gggtcacccg
     1141 tgccggcccg tccgggtcgc ccgcgccatc ggagaggacc tcgacccgga acaccggcgt
     1201 gcggccggct tcgtcggcga cgaagaacac cgccgagccg tccggcgccc agaccggctg
     1261 ctccggccac agcccgaagc cgggcagcag gtcgcggccc tgcccactgg ccagatccac
     1321 cagccacaag gtcacgtccg gcgactcgtc gggggtgccg tgccaggcgc gctggcacac
     1381 caccatgcgc ccctccgggg aacaggcgac cgcgtcgacc gacgacgcca cgtcatcgaa
     1441 cagcacccgg cgctcgccgc tggcggtggc gagcgcggcg agctgggtcc gggggttgcg
     1501 gacatcgtca tgcggcgccc aggtcgtcag gatcgtggcg ccgtccgggg tgagggcgaa
     1561 gctcgcctcg tccagcgccc gccccgccgc cgcaaccagc ggcgcgccgg ctgcgaggcg
     1621 gccctcgtcg gcggcgggcg gcgc
//
LOCUS       DASWDC010000020        15061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1006293, whole genome shotgun sequence.
ACCESSION   DASWDC010000020 DASWDC010000000
VERSION     DASWDC010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15061
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006293"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..882
                     /locus_tag="VF995_00610"
     CDS             172..882
                     /locus_tag="VF995_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX9342100.1"
                     /translation="MARVGLLVPSSNTVMEPDLWRALPVGATLHTGRMYLEDTTPEGE
                     HRMLDQHVLPAARDLATARPDLIIFGCTSAGALRGNAADADLCERVTEVSGVPTVSVI
                     ASVRRALAATGARRVGVVTPYVDALNQPIRASLEADGIEVAHIAGLGISDNVRIAEVP
                     AAEVVDFAVRELRGLPIEAVFVSCTNFPAVAALPELERRFGVPVVTSNQAAIAALLDL
                     LAASAAPAAAPADGGPRP"
     gene            894..1778
                     /locus_tag="VF995_00615"
     CDS             894..1778
                     /locus_tag="VF995_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX9342101.1"
                     /translation="MVAAPFRYQRAASFEEAVAALAEHGEDAKLLAGGQSLVPMLNLR
                     VARPEWLIDLNPVGAGPVAEAGGVLRLPALTRHRVLESDGLVRRRCPLLAEAASLVGN
                     VRTRARGTIGGSLAHADPAGELPLAALTLGAEVSVLGPAGARDLLVDELLISYLTTAL
                     APDEVITEVRVPAPRPRQGWSFLELTRRTSDWMVAGAAALVELDERGTVAAASLAFGG
                     VAERPVAAPAELLGGLYGEPPAEQRFARVADLAAATLRPEDDVHASGTYRRRVAGVLG
                     RRALNQAAIRARASGETP"
     gene            1775..2257
                     /locus_tag="VF995_00620"
     CDS             1775..2257
                     /locus_tag="VF995_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9342102.1"
                     /translation="MTDGDKQRVSFTVNGEAREAWVFPHESLLDVLRDAGCHEVRYGC
                     GEGVCGTCTVLLDGEPVSACLLFAVQAEGCSLLTVRGLAQGAAQPGEPHPLQASFLRN
                     GAAQCGFCTPGMLLTAWALLRREPHPTRERIRRELCGNLCRCTGYSKILDAIEDCRQP
                     "
     gene            2254..4593
                     /locus_tag="VF995_00625"
     CDS             2254..4593
                     /locus_tag="VF995_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9342103.1"
                     /translation="MTETTVPTREGYRIVGRSPAHHDFADKVAGSLLYAADWQLPGML
                     HGKVVRAQVPSARITAMDVSAARALPGVVAVLTAADVPWNQIVEHASGGLGELAVAMP
                     VLAADRVRYQGEPVALIAAVSPQAAEEAAELIDVDYQDLPGVFEVEAALEPGAPLVHD
                     EGNLLVTWNIGGDAAEVDAAMAAADLVVEGAYRTQRVEHAYLEPEAGVGWVETGGIIT
                     LRVATQVIEHAGAIAAILGLPANQVRVISSYMGGGFGGKEDMTVEPYLALLVWHTRRP
                     VRLVFERQESLLATTKRHPFVMRYRTGVRRDGTIVAQDVDIVGDAGAYPYLSARVLFA
                     ACVCASGPYRVPMVRHRARAVFTNTPPTSAFRGFGAMQVTFAYEQQLDRVAAALGLDP
                     VAVRERNFLAKGDAIPTGERLDTAVAVGECVRRALDALGEPPRPSGPGRRVGRGFAAN
                     MQPYGRTMWFRDRAACWLSLEADGSLQLRSGVTDLGGGQAASLAQIVSEILGVPLGEI
                     GVHIGDTALTPPAGGTFATRQLYMSGNAALAAARELRARLLPVAAALLGTAEDDLVFE
                     DGMVRPPGGSRGLRLAELAAACHQRGVPTSHLGTWYAPTGAFDPASGQGSSFPDYTFG
                     AHAADVEVDLETGQTRVLSYAAAHDVGRAINPLRVEGQIQGGAYQGIGYALGEEIVLA
                     DGNATSTLLANYLMPNAVDLPDVRVVVLESGEGKGPFGARGIGEPSIANAAATIANAV
                     AAATGARVTQLPITPERLLGALDALAVETDTVEGGPHAS"
     gene            4583..5857
                     /locus_tag="VF995_00630"
     CDS             4583..5857
                     /locus_tag="VF995_00630"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HEX9342104.1"
                     /translation="MHPDRSRREVVLNRAARELPPGPPRPLSWAPVALHQRLPGYAVT
                     PLREVPALARACGVGRVFVKDESARLGLPSFKMLGASWATYRAVAARLAARLGGPVDG
                     LDRLDQPRPWTSLGELGERLRPLGPLTLVAATDGNHGRAVARMAALLGYGAEIYLPAG
                     TASARIAAIEGEGAQVAVVGGTYDDAVATAIAAATVDERRLLIQDTAQSPDKQVPAWV
                     VEGYATVFLEVDQQVAELDAAPGVVLIPVGVGALAATAVRHWRRPELDAARRPRLVGV
                     EPLRAACVLRSIEAGRLVAVPGPHDSIMAGLNCGTPSPVAWPYLRGGLDAVVAVADER
                     ARQAMRDLAALGMVAGETGAAALAGLAELAGAGAGADEATRRALGLGPDASVLLLCTE
                     GATDPDAYQRIVAGKTKAKAKAETETETETRS"
     gene            5860..6867
                     /locus_tag="VF995_00635"
     CDS             5860..6867
                     /locus_tag="VF995_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX9342105.1"
                     /translation="MAESPYGGPLISLDGAVDLHCHPYPDLFPRLADDLDIALAARDA
                     GMKALVLKCHHESTVSRAYLVQRMVPGIRVFGGVVLNSYVGGVNPAAVEAALRLGGKE
                     VWMPTVDAGYHAQVHGGTGGYDNQQGGRQAGEGIWVTDREGRLRPEVADVLALVAEHR
                     AILGTCHLSPAEIVALVREARDVGVEKIVVTHPFFKVPNLDLDTLEELARLGAMPEFG
                     YCTISPAWQYAAPAKIVAAVQRVGAERCLLVSDTGQRHNPLPSEALRVFAQTVFEKGL
                     SEAEVTTMTARNPSDLLDLDSAAPAPRPVDRAWLARLGAHADPCVEAAGEADGEPAQR
                     R"
     gene            7075..7608
                     /locus_tag="VF995_00640"
     CDS             7075..7608
                     /locus_tag="VF995_00640"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9342106.1"
                     /translation="MNTSGTYQIGPHNGRLLIRTFREGVASMVGHDLVIEATSWRGTV
                     TVPEGGQPHIAVEVDVRSLEIREGLRGAKPLSDKDVRDIKETMADLLGAEYHPLVSYV
                     SNSVQVMGSSATADGQFTLLGITQPLRLALQVQDGVVTATTTVQQSLWGIKPFKAFMG
                     ALKVRDDVEVEATVRLG"
     gene            7750..8004
                     /locus_tag="VF995_00645"
     CDS             7750..8004
                     /locus_tag="VF995_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342107.1"
                     /translation="MSLGIGLFLLIVGAILTFAVNVTASGFNIHTVGLILMAGGALGI
                     LLWMAFWSSFAPMSRRPPPPLGDRDSYTERRVERIERDRY"
     gene            complement(8036..8842)
                     /locus_tag="VF995_00650"
     CDS             complement(8036..8842)
                     /locus_tag="VF995_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007388852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9342108.1"
                     /translation="MSNQGQSREHPFLDTSRPHPARMYDYFLGGKDNFPADREAGDEV
                     LTAWPEVREGVRENRAYLRRVVSFLAREAGIRQFLDIGTGIPTQGNVHEVAQAVVPDA
                     RVVYVDNDPIVHVHANALLAGETTSIILADLREPETILDHPEVRQAIDFGEPFAVLLL
                     AILHFVSDDEDPARIVAAFRKAMSPGSFLAISHVTADFSPETTERAARVYDRAAARLI
                     PRGGAEIGRFFDGLDLVEPGLVPIQRWRPRGQPAQLRGGMYGGVGRKPSG"
     gene            complement(8907..10658)
                     /locus_tag="VF995_00655"
     CDS             complement(8907..10658)
                     /locus_tag="VF995_00655"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9342109.1"
                     /translation="MGQVSRRHFLGYTAATGALAAAAPGLPSAEFEFEEATIAQLQAA
                     MEAGRLTSRRLTESYLQRIARIDLAGIRLNSVIEVNPDALAIAGTLDQERRQHGARGP
                     LHGIPVLVKDNYATDDRTQTAAGSLALVGSTVPRDAFVVRQLRAAGAVILGKANLSEW
                     ANFRSLQSSSGWSGRAGQCLNPYVLDHNPCGSSSGSGIAASASLAAGTLGTETDGSIV
                     CPSATCGVVGIKPTLGLTSRAGVVPLAHSQDVTGPICRTVADAATVLGGMTGVDPRDP
                     ATAASRGHSFTDYRRFLDPNGLRGARIGVWREGVFGVTPEGDAVAEAAISQLGKLGAT
                     IVDPADIPNVADIGGPEFTVLLFEFKADIVRYLSELSGTSMRTLADLIAFNNAHAGQE
                     LRWFGQEIFLLAEATTNLSDPTYLQALATSKKLAQDGINQTLARFDLDAIVSLTGGPS
                     WTTDLVNGDHFLTASSTPAAVAGYPSITVPAGFSFELPVGISFIGPRWGEPALIKLAY
                     GFEQGTRARRVPKFLPTFATRDFVPRDTSAGARSDPAAARPPSRARQGQGLAPEVGEG
                     KNKGRPHQHQNRLAGLL"
     gene            complement(10812..11606)
                     /locus_tag="VF995_00660"
     CDS             complement(10812..11606)
                     /locus_tag="VF995_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9342110.1"
                     /translation="MIAGVVFLADRLEKPILAEGPAGVGKTELAKALAAATGARLIRL
                     QCYEGLDESKALYEWNYKKQLLRIQADQAHEASWDNLEDDLFSEPFLLTRPLLEAIRA
                     TDPVVLLIDEVDRVEVETEALLLEVLSDFQVSIPELGTITGRQRPLIVLTSNATRELS
                     EALRRRCLYLHIDYPSVEREREIVRVRVPAIDDQLATQIARMVRRVRQLDLKKPPSIS
                     ETIDWARTLLHLGADSLEPAVVDDTLHLLLKHQRDIQRARAELRRS"
     gene            complement(11691..12686)
                     /locus_tag="VF995_00665"
     CDS             complement(11691..12686)
                     /locus_tag="VF995_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HEX9342111.1"
                     /translation="MATLETEPTVEIQASEPNEAGYRGFQLGEFRFRRDDYFAYIDWP
                     TGSHVMSADAFLKALQRDVAWDFFYGIVNFDGVIGTVNHYGTVDLFAGRYNDGYRKAE
                     LDHLENLATPIIRETFKAMLDDWTNEGFDPFASPDETGSAFGPKHGNNTRAVTRHRVT
                     AQRMVNAPGDEPLRSDESGFPVNRQFTDVPQDEPELRPAPGYEEEVRAFNLFKYLSRS
                     DVTWNPSVVSVCKDSLFCPTTEEYILPIIHGNDRVEWFVQLSDEIYWEVEDRDSGQIR
                     SKVLMKPGDVAAMPADIRHQGYAPKRSMLLVWENGDAELPALISSGKLPTNPIDF"
     gene            complement(12715..13266)
                     /locus_tag="VF995_00670"
     CDS             complement(12715..13266)
                     /locus_tag="VF995_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HEX9342112.1"
                     /translation="MPQAELAMAGQKGVVGLSRFTTKFGSLDNYEKGHVEIVDDDPRH
                     YAFSNIYEVATRSKPYEKVAVGMNRQYVLEAIRAEGTSGWRTCSHDEFALVMDGEVHV
                     ELAKLGDGQQPDPEVDGSVALEGEPGGPAMGEIVARRGHMALLPVGAAYRFHADHPGV
                     ILLQTIAGRDTLYRWADICQVTP"
     gene            13338..14108
                     /locus_tag="VF995_00675"
     CDS             13338..14108
                     /locus_tag="VF995_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016722797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX9342113.1"
                     /translation="MSTRADRDFVQSLERGLAVLQVFSRERPSVSLSEAAELTGLTRA
                     TARRILLTLERLGYVRAEGRQFRPTPRVLAIGYAYLSSLDLWQAAHPFMAELADLTQE
                     SCSASQLDGNEVVYVARVPSSRVMSVNLNVGARVPAYPTSMGRVLLGGLGPAALDRYF
                     AEVEPRPLTPNTVTDEARLRQIVAECHTQGWSLVDEELEAGLRSIAAPIHDHSGRILA
                     ALTICGHAGRVTPAQLRDEFLPHLLEAAKQISDQVRRR"
     gene            complement(14083..15018)
                     /locus_tag="VF995_00680"
     CDS             complement(14083..15018)
                     /locus_tag="VF995_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342114.1"
                     /translation="MDPFRPTLPPPGRLVRLSVVLTHQNSPERLLQLARMCDRMCGRA
                     GIGAIWIEPGDPTEARGVLESVAAATDRIRLGLHAALPPGSEGSEGSGAALEALATSL
                     VAACGDRAELSLRGRPAPIARSAFHTDLTFSVEAEPADPAALARWLTVADDLLLPAGP
                     VDRVAEAASAVRRACAAAGRDPATLGIAARLPVSVGRTIAEAWARWEADPAFAALGDP
                     AEAAVFGTLEQCHDRVITLAHAGVTDLRCLLPNTPDVHDVIAQITAMTIGTVDKLLPG
                     APRSPAPPPPAGWGGRPRFPSGSASTGLSGGGPGR"
BASE COUNT         2032 a         5480 c         5440 g         2109 t
ORIGIN      
        1 agccggacag caggcggatg cgcctgcgcg ccctgcagcc aggcgtgacc gccgagcggg
       61 tccaggccgc gaccggcttc gagctgctcg tccacccgga agtgcacgag ctggagccgc
      121 cgtcaatgga ggacctggtg atcctctacg agctggaggg aggctgagcc gatggctcgg
      181 gtgggattgc tggtcccgtc ctcgaacacc gtgatggagc cggatctctg gcgggcgctg
      241 ccggtggggg cgacgctcca caccggccgc atgtacctgg aggacaccac cccggagggc
      301 gagcaccgca tgctcgacca gcacgtgctg ccggccgccc gcgacctcgc caccgccaga
      361 ccggatctga tcatctttgg ctgcaccagc gccggggccc tgcgcggcaa cgccgccgac
      421 gccgacctgt gcgagcgcgt cactgaggtg tccggcgtgc ccaccgtgag cgtgatcgcc
      481 tcggtccggc gggcgctcgc cgccaccggc gcgcgccgcg tcggcgtggt caccccgtat
      541 gtggacgcgc tcaaccagcc gatccgcgcg agcctggagg ccgacggcat cgaggtcgcg
      601 cacatcgcgg ggctcggcat cagcgacaac gtccgcatcg ccgaggtgcc ggccgccgag
      661 gtcgtggact tcgccgtgcg ggagctgcgt ggcctcccga tcgaagcggt gttcgtctcc
      721 tgcaccaact tcccggcggt ggcggccctg cccgagctgg agcgccgctt cggcgtcccg
      781 gtggtgacca gcaaccaggc ggcgatcgcg gcgctgctcg acctgctcgc cgcctcggcc
      841 gccccggcgg cggcccccgc cgacggcggg ccccggccgt gagggggcgc tgagtggtcg
      901 ccgcgccgtt ccgctaccag cgtgccgcct cgttcgagga ggcggtggcg gcgctcgccg
      961 agcacggcga ggacgccaag ctgctcgccg gcgggcagag cctggtgccg atgctgaacc
     1021 tgcgggtcgc ccgacccgag tggctgatcg acctcaaccc ggtcggcgcc gggccggtcg
     1081 ccgaggccgg cggggtgctc cgcctgcccg ccctgaccag gcaccgggtg ctcgagtccg
     1141 acgggctggt gcggcggcgc tgcccgctgc tggccgaggc ggccagcctc gtcggcaacg
     1201 tccgcacccg ggcgcgcggc accatcggcg gcagcctcgc gcacgccgac ccggcggggg
     1261 agctgcccct ggcggcgctc accctcggtg ccgaggtcag cgtcctcggc ccggcaggtg
     1321 cccgggacct gctggtggac gagctgctga tctcgtatct gaccaccgcc ctcgccccgg
     1381 acgaggtcat caccgaggtc cgggtgccgg cgccgcggcc ccgccagggc tggtcgttcc
     1441 tggagctgac ccgccgcacc tccgactgga tggtggccgg cgcggcggcg ctggtggagc
     1501 tggacgagcg cggcacggtc gccgccgcct ccctcgcctt cggcggcgtg gccgagcgcc
     1561 cggtcgccgc gcccgcggag ctgctcggcg gcctgtacgg cgagccgccc gccgagcagc
     1621 ggttcgcccg ggtcgccgac ctggccgcgg ccaccctgcg gcccgaggac gacgtccacg
     1681 cctccggcac ctaccggcgg cgggtcgccg gggtcctcgg gcgtcgcgcc ctcaaccagg
     1741 cagccatccg ggcacgagcg agcggggaaa cgccatgacg gacggcgaca agcagcgggt
     1801 cagcttcacc gtcaacggcg aggcgcgcga agcctgggtg ttcccccacg agagcctcct
     1861 ggacgtgctc agggacgccg gctgccacga ggtccgctac ggctgcggcg agggggtgtg
     1921 cgggacctgc acggtgctgc tcgacggcga gccggtgagc gcctgcctgc tgttcgcggt
     1981 gcaggcggag ggctgctcgc tgctgaccgt gcgcggcctc gcccagggcg ccgcccaacc
     2041 cggcgagccg catccgctgc aggcgagctt cctgcgcaac ggggccgccc agtgcggctt
     2101 ctgcaccccc gggatgctgc tgaccgcgtg ggcgctgctg cggcgcgagc cgcacccgac
     2161 ccgcgagcgg atccggcggg agctgtgcgg caacctctgc cgctgcaccg gctacagcaa
     2221 gatcctcgat gcgatcgagg actgccgcca gccatgaccg agaccaccgt cccgacccgg
     2281 gagggctacc ggatcgtcgg gcgctcgccc gcccaccacg acttcgccga caaggtcgcc
     2341 gggtcgctgc tgtacgccgc cgactggcag cttcccggca tgctgcacgg caaggtggtg
     2401 cgcgcccagg tgccgtccgc ccgcatcacc gccatggacg tctcggccgc gcgggcgctg
     2461 cccggcgtgg tcgccgtcct gaccgccgcc gacgtgccct ggaaccagat cgtggagcat
     2521 gccagcggcg ggctcggcga gctggcggtc gccatgccgg tgctggcggc cgaccgggtc
     2581 cgctaccagg gcgagccggt cgcgctgatc gccgccgtct cgccccaggc cgccgaggag
     2641 gcggccgagc tgatcgacgt cgactaccaa gacctgccgg gcgtcttcga ggtggaggcc
     2701 gccctggaac cgggcgcgcc gctggtccac gacgagggga acttgctggt cacctggaac
     2761 atcggcggcg acgcggcgga ggtggacgcc gccatggccg cggccgacct ggtggtcgag
     2821 ggcgcctacc gcacccagcg ggtcgagcac gcctacctgg agcctgaggc cggggtcggc
     2881 tgggtcgaga ccggcgggat catcaccttg cgggtcgcca cccaggtgat cgagcacgcc
     2941 ggcgccatcg cggcgatcct cgggctgccc gccaaccagg tccgggtcat ctccagctac
     3001 atgggcggcg gcttcggcgg caaggaggac atgaccgtcg agccctacct cgccctgctg
     3061 gtgtggcaca cccgccggcc ggtcaggctg gtcttcgagc gccaggagtc gctgctggcc
     3121 accaccaagc gccacccgtt cgtgatgcga taccgcaccg gggtccggcg cgacggcacg
     3181 atcgtcgccc aggacgtcga catcgtcggc gacgccggcg cctaccccta cctgagcgcg
     3241 cgggtgctgt tcgccgcctg cgtctgcgcc tccggcccct accgggtgcc aatggtccgc
     3301 caccgggccc gcgcggtctt caccaacacc ccgccgacca gcgccttccg cggtttcggc
     3361 gcgatgcagg tgaccttcgc ctacgagcag cagctggacc gggtcgcggc ggcgctcggc
     3421 ttggacccgg tcgcggtgcg ggagcgcaac ttcctcgcca agggcgacgc catcccgacc
     3481 ggcgagcggc tcgacaccgc ggtcgcggtg ggggagtgcg tgcgccgcgc cctcgacgcc
     3541 ctcggcgagc cgccgcggcc gagcggcccg gggcggcggg tcgggcgggg gttcgccgcc
     3601 aacatgcagc cctacggccg caccatgtgg ttccgcgacc gtgccgcctg ctggctcagc
     3661 ctggaggcgg acggcagcct gcagctccgc agcggcgtca ccgatctcgg cggcggccag
     3721 gcggcctcgc tggcgcagat cgtctccgag atcctcggcg ttcccctcgg cgagatcggg
     3781 gtgcacatcg gcgacaccgc cctgaccccc ccggccggcg ggaccttcgc gacccgccag
     3841 ctctacatgt ccggcaacgc tgccctcgcc gccgcccgcg agctgcgcgc ccggctgctg
     3901 ccggtcgccg ccgcgctgct cggcaccgcc gaggacgacc tggtcttcga agacggcatg
     3961 gtccggcccc ccggtggatc aaggggcctg cgcctggccg agctggccgc cgcctgccac
     4021 cagcgcgggg tgccgacctc gcatctcggc acctggtacg ccccgaccgg agccttcgat
     4081 ccggccagcg gccagggctc cagctttccc gactacacct tcggtgccca tgccgccgac
     4141 gtggaggtcg acctggagac cgggcagacg agagtgctca gctacgccgc cgcccacgac
     4201 gtcggccgcg cgatcaaccc cctgcgggtc gagggccaga tccagggcgg cgcctaccag
     4261 ggcatcggct acgccctcgg cgaggagatc gtgctggccg acggcaacgc cacctcgacc
     4321 ctgctggcca actacctcat gccgaacgcg gtcgacctcc ccgacgtccg cgtggtcgtc
     4381 ctcgaaagcg gcgagggcaa gggccccttc ggcgcccgcg gcatcggcga gccctcgatc
     4441 gcaaatgccg ccgccaccat cgccaacgcc gtcgccgccg ccaccggcgc ccgcgtcacc
     4501 cagctcccca tcaccccgga gcgcctcctc ggcgccctcg acgcgcttgc tgtggagacg
     4561 gatacggtgg agggcggacc gcatgcatcc tgaccgatcg cgccgcgagg tcgtcctcaa
     4621 ccgggcggcc cgggagctgc cgccagggcc gccgcggccg ctgtcgtggg cgccggtggc
     4681 attgcatcag cggctccccg gctacgcggt cacgccgctg cgggaggtcc ccgcgctggc
     4741 gcgggcgtgc ggagtcggcc gggtcttcgt caaggacgag tccgcccggc tcggcctccc
     4801 ctccttcaag atgctcggcg cgagctgggc gacctaccgg gcggtcgccg cccgcctcgc
     4861 cgcccgcctc ggcgggcccg tggacggcct cgatcgcctg gaccagccac ggccgtggac
     4921 gagcctgggc gagctgggcg agcggctccg cccgctcggg ccgctgacgc tcgtggccgc
     4981 caccgacggc aaccatggcc gcgccgtcgc ccggatggcc gccctgctcg gctacggcgc
     5041 cgagatctac ctgccggccg gcaccgccag cgcgcggatc gccgcgatcg agggggaggg
     5101 agcacaggtc gccgtggtcg gcggaaccta cgacgacgcg gtcgccaccg ccatcgccgc
     5161 cgcgaccgtg gacgaacgcc gcctgctgat ccaggacacc gcccagtccc cagacaagca
     5221 ggtccccgcc tgggtggtcg agggctacgc gaccgtcttt ttggaggtcg accagcaggt
     5281 cgccgaactg gacgccgcgc ccggcgtggt gctgatcccg gtgggggtgg gcgcgctcgc
     5341 cgccaccgcg gtgcggcact ggcggcggcc ggagctggac gcggcgcggc ggccgcggct
     5401 ggtcggcgtc gagccgctgc gcgccgcgtg cgtgctgcgc tcgatcgagg cggggcgcct
     5461 ggtcgccgtc cccgggccgc atgactcgat catggcgggg ctcaactgcg gcaccccctc
     5521 gccggtcgcc tggccctacc tgcgcggcgg cctcgacgcg gtggtggcgg tcgccgacga
     5581 gcgcgcccgc caggcgatgc gcgacctcgc cgcgctgggc atggtcgccg gggagaccgg
     5641 cgcggcggcg ctcgccgggc tcgcggaact cgccggggcc ggggccgggg ccgacgaggc
     5701 cacacggcgg gcgctcggcc tcgggcctga tgccagcgtg ctgctgctct gcaccgaagg
     5761 cgcgaccgac ccggacgcct accagcgcat cgtcgccggc aagaccaagg ccaaggccaa
     5821 ggccgagacc gagaccgaga ccgagacaag gagctgaccg tggcagagag tccgtatggc
     5881 ggcccgctga tctccctcga cggtgcggtc gacctgcact gccatcccta cccggacctg
     5941 ttcccgcggc tcgccgacga cctggacatc gcgctggccg cgcgggacgc gggcatgaag
     6001 gcgctggtgc tgaagtgcca ccacgagagc accgtgtccc gcgcctacct ggtccagcgg
     6061 atggtgcccg ggatccgggt gttcggcggg gtggtgctga actcctatgt cggcggcgtc
     6121 aaccccgccg cggtcgaggc ggcgctgcgg ctcggcggca aggaggtgtg gatgccgacg
     6181 gtggacgccg gctaccacgc ccaggtccac ggcggcaccg gcggctacga caaccagcag
     6241 ggcggccgcc aggcaggaga ggggatctgg gtcaccgacc gggagggccg gctccggcca
     6301 gaggtcgccg acgtcctcgc cctggtcgcc gagcaccgcg ccatcctcgg cacctgccac
     6361 ctgtcccctg ccgagatcgt ggcgctggtg cgggaggcgc gcgacgtcgg cgttgagaag
     6421 atcgtggtca cccacccatt cttcaaggtg cccaacctcg acctcgacac cctggaggag
     6481 ctggcccgcc tgggcgccat gccggagttc ggctactgca ccatctcgcc cgcctggcag
     6541 tacgcggcgc ccgccaagat cgtcgcggcg gtccagcggg tcggcgccga gcgctgcctg
     6601 ctggtgtccg acaccggaca acggcacaat ccgctcccct ccgaggcgct gcgggtcttc
     6661 gcccagacgg tcttcgagaa gggcctcagc gaggctgagg tgaccaccat gacggcccgc
     6721 aacccgagcg acctgctgga cctggacagc gccgccccgg cgccccgccc ggtcgaccgg
     6781 gcctggctgg cgcgtctcgg cgcccacgcc gacccgtgcg tggaggccgc cggggaggcc
     6841 gacggggagc cggcgcagcg gcgttagggc ctcaggcgct ctcgggccgc cgccgcatgc
     6901 agtagtaggc gggcgcgagg aagctccggg gagccacgtc gccttccacg aagcgggcct
     6961 ggaccccggc ggcgctcctc aatcaggaac aggacgagcc gaacgctcag ggtgagtgat
     7021 cactacgtta tgtgaagttg gcaaaatcgt tacatagcag tagtgtcccg ccccatgaac
     7081 acatctggga cgtaccagat tggcccccat aacgggcgcc tgctgatccg caccttccgt
     7141 gagggtgtcg cctccatggt tgggcacgac ctcgtcatcg aggcgaccag ctggcgggga
     7201 accgtcaccg tcccggaagg cggacagccg cacatcgccg tcgaggtgga cgtgcgctcc
     7261 ctggagatcc gcgagggcct caggggcgcg aagccgctca gcgacaagga cgtgcgggac
     7321 atcaaggaga ccatggccga cctgcttggg gccgaatacc atccgctggt ctcctacgtg
     7381 tccaactcgg tccaggtcat gggcagcagc gccacggccg acggccagtt caccctgctc
     7441 ggcatcaccc agccgctgcg cctcgcgctc caggtgcagg acggcgtggt caccgcgacc
     7501 acgaccgtcc agcagagcct ctggggcatc aagccgttca aggcgttcat gggtgccctc
     7561 aaggtccgcg acgacgtcga ggtcgaagcg accgtgaggc tcgggtaggg acccccccag
     7621 acgtcccgtc cggacgggag ccatccccca ggctcccgtc cgctgttttc cctcctggtt
     7681 tccgctgggg acgggcgggt agccgccaag acgagcgacc acacacccaa ctcaggagga
     7741 gtggtggtca tgagccttgg tatcggtctc ttcctgctga tcgtgggggc catcctgacc
     7801 ttcgcggtca atgtcaccgc cagcgggttc aacatccaca ccgtcggcct catcctgatg
     7861 gcgggcggcg ccctcggcat cctgctctgg atggccttct ggagcagctt cgccccgatg
     7921 agccgccgcc ctccgccgcc cctcggcgac cgcgactcct acaccgaacg ccgcgtcgag
     7981 cggatcgagc gggaccgcta ctgagcccgc cggtcgccgt cgcccagcct gcccatcagc
     8041 cggagggctt gcggccgacc ccgccgtaca tcccgccccg gagctgggcg ggctggcccc
     8101 gcggccgcca ccgctggatc gggaccaggc cgggttcgac caggtcgagg ccgtcgaaga
     8161 agcggccgat ctcggcgccg ccgcggggga tcagccgggc cgccgcccgg tcgtagactc
     8221 gcgcggcccg ctcggtggtc tccgggctga agtcggcggt cacgtgcgag atcgcgagga
     8281 agctccccgg gctcatcgcc ttccggaacg ccgccacgat cctcgccggg tcctcgtcgt
     8341 cgctgacgaa gtgcaggatg gccagcagca gcaccgcgaa cggctcgccg aagtcgatcg
     8401 cctgccgcac ctcgggatgg tcgaggatcg tctcgggctc gcgcaggtcg gccaggatga
     8461 tgctggtggt ctcgccggcc agcagcgcgt tggcgtggac gtggacgatc gggtcgttgt
     8521 ccacgtagac cacccgcgcg tccggcacga ccgcctgcgc gacctcgtgg acgttcccct
     8581 gggtcgggat gccggtgccg atgtcgagga actggcggat ccccgcctcc cgggccagga
     8641 agctgaccac ccggcgcagg tacgcccggt tctcgcggac cccctcgcgc acctccggcc
     8701 aggcggtcag gacctcgtcg cccgcctccc ggtcggccgg gaagttgtcc ttgccgccga
     8761 ggaagtagtc atacatccgc gccggatgcg gccggctcgt atcaaggaac ggatgctccc
     8821 ggctctggcc ctggttgctc atgacgccgc ctccgtccgg cccgcggcgt cacccccgga
     8881 cgcccggttc cgccggaggc gtcaccttac aacaagccgg cgagccggtt ctggtgctgg
     8941 tgcggccggc ccttgttctt cccctcgccc acctcggggg cgaggccctg cccctggcga
     9001 gcccgcgatg gggggcgggc ggcggccggg tcgctgcggg cgccggcgct cgtgtcacgc
     9061 gggacgaagt ccctggtggc gaaggtcggc aggaacttcg gcacgcgccg ggccctggtg
     9121 ccctgctcga agccgtaggc gagcttgatc agcgccggct cgccccacct cgggccgatg
     9181 aagctgattc ccaccggcag ctcgaacgag aacccggcgg gcacggtgat gctcgggtag
     9241 cccgccaccg ccgccggcgt cgagctggcg gtcaggaagt ggtcgccgtt gaccagatcg
     9301 gtggtccagg aaggaccgcc ggtcaggctc acgatcgcgt ccaggtcgaa cctggccagg
     9361 gtctggttga tcccgtcctg cgccagcttc ttcgaggtgg ccagggcctg cagataggtg
     9421 gggtcagaga ggttggtggt cgcctcagcg agcaggaaga tctcctgccc gaaccatcgc
     9481 agctcctggc cggcgtgggc gttgttgaag gcgatcaggt cggcgagggt gcgcatcgag
     9541 gtgccggaca gctccgacag gtacctgacg atgtccgctt tgaactcgaa cagcagcacg
     9601 gtgaactcgg gcccgccgat gtcggccaca ttcgggatgt cggccgggtc gacgatcgtg
     9661 gcgccgagct tgccgagctg gctgatggcc gcctcggcga cggcgtcgcc ctccggcgtg
     9721 acgccgaaca cgccctcgcg ccacaccccg atgcgggcgc cgcgcagccc gttggggtcc
     9781 aggaagcggc ggtagtccgt gaacgaatgg ccccggctgg cggcggtcgc cgggtcgcgc
     9841 gggtccacgc cggtcatgcc gccgagcacg gtggcggcgt cggccacggt gcggcagatc
     9901 ggcccggtga cgtcctggct gtgcgccagc ggcaccaccc cggcgcggct ggtgagcccg
     9961 agggtcggct tgatcccgac gaccccgcag gtcgccgacg ggcagacgat cgacccgtcg
    10021 gtctcggtcc cgagcgtccc ggcggccagg ctggccgagg cggcgatccc cgacccggag
    10081 ctggacccgc acgggttgtg gtccagcaca taggggttga ggcactggcc ggcacggccc
    10141 gaccagccgc tcgacgactg cagcgagcgg aagttggccc attccgacag gttcgccttg
    10201 ccgaggatca ccgctcccgc cgcgcgcagc tgccgcacca cgaaggcgtc ccgggggacc
    10261 gtcgacccga ccagcgcgag ggagccggcc gccgtctgcg tgcggtcgtc ggtggcgtag
    10321 ttgtccttca ccaggacggg gatgccgtgc agcgggccgc gcgcgccgtg ctggcggcgc
    10381 tcctggtcga gcgtccctgc gatggcgagc gcgtccgggt tcacctcgat caccgagttg
    10441 agccggatcc cggcgagatc gatccgcgcg atccgctgga gatacgattc cgtgaggcgg
    10501 cgggaggtga gccgccctgc ctccatcgcc gcctggagct gcgcgatggt cgcctcctcg
    10561 aactcgaact ccgcggacgg cagacctgga gccgccgcgg cgagcgcgcc ggtagctgcg
    10621 gtgtagccga gaaagtgccg gcggctgacc tgacccattg ttggctccct tcgcccggca
    10681 atctgaccgt ttgcggcatc acaccctaaa gaagcgagaa aggcaagatg agggaaacgt
    10741 ctgctcgctt cggtcgcgcg aagatccggg cggcccgacc agccccggga atcgcctccg
    10801 ctccaggccg gtcaggagcg ccgcagctcc gcccgggccc gctggatgtc acgctggtgt
    10861 ttgagcagca ggtggagggt atcgtccacc accgccggct ccagcgagtc cgccccgagg
    10921 tggagcagcg tgcgtgccca gtcgatggtc tccgagatcg acggtggctt tttgagatcc
    10981 agctggcgca ccctgcggac catccgcgcg atctgggtgg cgagctggtc gtcgatcgcg
    11041 ggcacccgga cacgcacgat ctcacgctcg cgctccacac tcggatagtc gatgtggagg
    11101 tagaggcagc gccgccgcag cgcctctgac agctcccggg tggcattgga ggtcagcacg
    11161 atcagcggcc gctggcgccc ggtgatcgtg cccagctccg ggatcgacac ctggaagtcg
    11221 gagaggacct ccagcagcag cgcctcggtc tcgacctcga cccggtcgac ctcgtcgatc
    11281 agcagtacca ccgggtcggt ggcgcggatc gcctccagca gcggccgggt cagcaggaac
    11341 ggctcggaga agaggtcgtc ctccaggttg tcccagctcg cctcgtgggc ctggtcggcc
    11401 tggatgcgca gcagctgctt cttgtagttc cactcgtaga gggccttcga ctcgtccagc
    11461 ccctcgtagc actgcagccg gatgagccgc gcgccggtcg cggcggcgag cgccttggcc
    11521 agctcggtct tgccgacgcc ggcggggccc tcggccagga tcggcttctc cagccggtcg
    11581 gccaggaaca ccaccccggc gatcatcgga ttggggaggt agccggcggc ggcgagccgc
    11641 gcctcgacct cggcggtgtc cgcgaacctg aaggccacca gccgacctga tcagaaatcg
    11701 atcgggttgg tgggcagctt cccgctggag atcagcgccg gcagctcggc gtcgccgttc
    11761 tcccagacca gcagcatcga ccgttttggc gcgtagccct ggtggcggat gtcggccggc
    11821 atcgcggcga cgtcgccggg cttcatcagc accttggagc ggatctggcc gctgtcgcgg
    11881 tcctcgacct cccagtagat ctcgtcggag agctgcacga accactcgac ccggtcgttg
    11941 ccgtggatga tcggcaggat gtactcctcg gtcgtcgggc agaacaggct gtccttgcag
    12001 accgagacca ccgacgggtt ccaggtcacg tccgagcgcg acaggtactt gaagaggttg
    12061 aaggcgcgga cctcctcctc gtacccgggc gccgggcgca gctccggctc gtcctggggc
    12121 acgtcggtga actgccggtt gaccgggaag ccgctctcgt cggagcgcag cggctcgtcg
    12181 ccgggggcgt tcaccatgcg ctgggcggtg acccggtggc gggtgacggc cctggtgttg
    12241 ttgccatgct tggggccgaa ggcgctgccg gtctcgtcgg ggctggcgaa ggggtcgaag
    12301 ccctcgttgg tccagtcgtc cagcatcgcc ttgaaggtct cgcggatgat cggcgtcgcc
    12361 aggttctcca ggtggtccag ttcggccttg cggtagccgt cgttgtagcg gccggcgaac
    12421 aggtccacgg tgccatagtg gttgacggtg ccgatcaccc cgtcgaagtt gacgatgccg
    12481 tagaagaagt cccaggccac gtcgcgctgc agcgccttga ggaaggcgtc ggcgctcatc
    12541 acgtggctgc cagtgggcca gtcgatgtag gcgaagtagt cgtcacggcg gaacctgaac
    12601 tcgccgagct gaaagcctcg gtagccggcc tcgttgggct cgctggcctg gatctcgacg
    12661 gtaggctcgg tctcgagcgt cgccataggt gctacctccc gctttccctt cgagctacgg
    12721 ggttacctgg cagatgtcgg cccaccggta gagcgtgtcc cggccggcga tggtctggag
    12781 gaggatcacg cccgggtggt cggcgtggaa gcggtaggcg gcgccgacgg ggagcagggc
    12841 catgtggccc cggcgggcga cgatctcgcc catggcgggg ccgccgggct cgccctccag
    12901 cgcgaccgag ccgtccacct cggggtcggg ctgctggccg tcgccgagct tggccagctc
    12961 gacgtggacc tcgccgtcca tgaccagcgc gaactcgtcg tgggagcagg tgcgccagcc
    13021 ggaggtgccc tcggcgcgga tcgcctccag cacgtactgg cggttcatgc cgaccgccac
    13081 cttctcgtac ggcttggagc gggtggcgac ctcgtagatg ttcgagaagg cgtagtgcct
    13141 gggatcgtcg tcgacgatct cgacgtggcc cttctcgtag ttgtccaggg agccgaactt
    13201 ggtggtgaaa cgggacaagc cgaccactcc cttctggcct gccatcgcca gctctgcctg
    13261 cggcatgcta gtgtgcgcga tgcgaacgtt tgtacgtcta tgcgcacagt tgcagattct
    13321 cagcggggga gggagcggtg tcaaccagag cggaccggga tttcgtgcag tccctcgaac
    13381 ggggcctcgc ggtcctgcag gtgttctccc gggagcgccc gtcggtctcg ctgagcgagg
    13441 cggcggagct gaccggcctc acccgggcga ccgcccgccg catcctgctc accctggagc
    13501 gtctcggcta cgtccgcgcc gagggccgcc agttcaggcc caccccccgg gtgctcgcta
    13561 tcggctacgc ctacctgtcg tcgctggacc tgtggcaggc cgcccacccc ttcatggccg
    13621 agctggccga cctcacccag gagtcctgct ccgccagcca gctcgacggc aacgaggtgg
    13681 tctacgtcgc ccgggtcccc tccagccggg tcatgagcgt caacctgaac gtcggcgccc
    13741 gcgtccccgc ctaccccacc tcgatgggcc gcgtcctgct cggcggcctc ggccccgccg
    13801 ccctcgaccg ctacttcgcc gaggtcgagc cgcgccccct cacccccaac accgtcaccg
    13861 acgaagcccg cctccgccag atcgtcgccg aatgccacac ccagggctgg tccctggtcg
    13921 acgaggagct cgaagccggc ctccgctcca tcgccgcccc catccacgac cactccggcc
    13981 gcatcctcgc cgccctcacc atctgcggcc acgccggccg cgtcaccccc gcccaactgc
    14041 gcgacgagtt cctcccccac ctcctcgaag ccgccaagca gatcagcgac caggtccgcc
    14101 gccgctgaga ccagtgctcg cggagcccga tgggaaccgc ggtcggccgc cccagcccgc
    14161 aggcggcggg ggagcggggg agcggggggc gcccgggagg agcttgtcga cggtgccgat
    14221 ggtcatcgcg gtgatctggg cgatgacgtc gtggacgtcg ggggtgttgg gcaggaggca
    14281 gcgcaggtcg gtgaccccgg cgtgagcgag ggtgatgacc cggtcgtggc actgctccag
    14341 ggtgccgaag acggcggcct cggccgggtc gccgagcgcg gcgaaggcgg ggtccgcctc
    14401 ccagcgggcc caggcttcgg cgatggtgcg gccgacggag accggcaggc gcgcggcgat
    14461 gccgagggtg gcggggtctc ggccggcggc ggcgcaggca cggcgcacgg ccgaggcagc
    14521 ctcagcgacc cggtcgactg gaccggcagg cagcagcaag tcgtcggcga cggtgagcca
    14581 tcgcgccagg gcagccgggt cagcgggctc ggcctccacc gagaatgtca ggtccgtgtg
    14641 gaaggcagac ctcgcgatcg gtgccgggcg tcctcggagt gacagttcgg cccgatcgcc
    14701 gcaggcagcg accagggagg tggccagggc ttccagggcc gccccggaac cctcggatcc
    14761 ttcggatccg ggagggaggg cggcgtggag gcccaggcgg atccggtcgg tcgccgcggc
    14821 cacggactcc aggaccccac gcgcttctgt ggggtcgccg ggctcgatcc agatggcgcc
    14881 gatgccggcg cggccgcaca tgcggtcgca catgcgggcg agctggagga gccgctcggg
    14941 cgagttctgg tgggtgagga ccacgctgag gcggacgaga cggccgggag gtggcagagt
    15001 gggacggaag gggtccacgg gccgttgaca tccgatgcgc gaagcgaaca gaagttcgcg
    15061 t
//
LOCUS       DASWDC010000021         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_336439, whole genome shotgun sequence.
ACCESSION   DASWDC010000021 DASWDC010000000
VERSION     DASWDC010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336439"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VF995_00685"
     CDS             <1..588
                     /locus_tag="VF995_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342115.1"
                     /translation="CGQGPDERSALAALAGSGGLDRDAMVVAERIEGVEAAFQRDRRP
                     AAPEETARTIEILKAAREDTIRLVRSATVAELDWRDPAAASERAPLAGTGPSWQTLRQ
                     AAWHLCDTESRVYLAALGEAPPRRSLDLLTELERSHQHVLTTLACLRRGRSRSLGTET
                     WTTVKVLRRLAWHERAELIPMHNLLARARTAALNY"
     gene            complement(653..>1712)
                     /gene="groEL"
                     /locus_tag="VF995_00690"
     CDS             complement(653..>1712)
                     /gene="groEL"
                     /locus_tag="VF995_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9342116.1"
                     /translation="EGMQFDKGYISPYFVTDPERMEAVFEDPYILIANQKVSSAQVLL
                     PVLEKVVQAGRPLVIISEDVDGEALATLVVNKIRGTFKSVAVKAPGFGDRRKAMLADI
                     AVLTGGEVISEEVGLKLENATLDLLGRARRVVITKDETTIVEGAGTEQDIKGRIAQIK
                     TEIERTDSDWDREKLQERQAKLSGGVAVVKVGAATEVELKEKKHRIEDALSATRAAVE
                     EGIVPGGGVALLRAQDALAKLDLTGDEATGVQIVRKSLELPLYWIASNAGLEGAVAVA
                     RVRDLDGAHGLNAATGEYVDLVKEGIIDPAKVTRSAVQNAASIAALLLTTEVLVVDKP
                     EEKADGGAGGGHNHGMGM"
BASE COUNT          218 a          621 c          607 g          266 t
ORIGIN      
        1 tgcgggcagg gccccgacga gcgctccgcg ctggccgcgc tggccgggtc aggcggcctc
       61 gaccgggacg cgatggtggt ggccgagcgg atcgagggcg tcgaggccgc cttccagcga
      121 gaccgccgcc ccgccgcccc ggaggagacg gcccgcacga tcgagatcct gaaggcggcg
      181 cgggaggaca cgatcaggct ggtccgctcg gccaccgtcg cggagctgga ctggcgcgac
      241 cccgccgccg cgtccgagcg ggcgccgctg gctgggaccg ggcccagctg gcagaccctc
      301 cgccaggcgg cctggcatct ctgcgacacc gagtcccgcg tctacctcgc cgccctcggc
      361 gaggcacccc cgcgccgcag cctcgacctg ctcaccgagc tggagcgctc ccaccagcac
      421 gtgctgacca cgctcgcgtg cctgcgccgg ggccgctccc gtagcctggg caccgagacc
      481 tggaccaccg tgaaggtcct gcgccgcctc gcctggcacg aacgcgccga gctgatcccg
      541 atgcacaacc tgctggcccg ggcgcggaca gcggccctca actactgaaa gcgggccccg
      601 gccacaggcg ccggagcccg ctccctgatg atcgggtgtg ggttgttcgt tgttacatgc
      661 ccatgccgtg gttgtggcca ccgccggcgc cgccgtcagc cttctcctcc ggcttgtcca
      721 ccacgagcac ctcggtggtg agcaggagtg cggcgatcga ggcggcgttc tgcaccgcgg
      781 agcgggtcac cttggctggg tcgatgatgc cttccttgac gaggtcgacg tactcgccgg
      841 tggccgcgtt gaggccatgg gcgccgtcca ggtcgcggac tcgggcgacc gccaccgcgc
      901 cttcgaggcc ggcgttgctg gcgatccagt agagcggcag ctccagcgac ttgcgcacga
      961 tctgcacgcc ggtcgcctcg tcgccggtca ggtccagctt ggccagcgcg tcctgcgccc
     1021 gcagcagcgc cacgccgccg ccggggacga tgccctcctc gaccgcggcg cgcgtggccg
     1081 agagggcgtc ctcgatgcgg tgcttcttct ccttcagctc gacctcggtg gccgcgccga
     1141 ccttgaccac ggcgacaccg ccggacagct tggcctggcg ctcctggagc ttctcgcggt
     1201 cccagtcgga gtcggtccgc tcgatctcgg tcttgatctg ggcgatgcgg cccttgatgt
     1261 cctgctcggt gccggcgccc tcgacgatgg tggtctcgtc cttggtgatg accacgcggc
     1321 gggcgcggcc gagcaggtcg agggtggcgt tctcgagctt gaggccgacc tcctcgctga
     1381 tgacctcgcc gccggtgagg acggcgatgt cggcgagcat cgccttgcgg cggtcgccga
     1441 agccgggggc cttgaccgcg accgacttga aggtgccgcg gatcttgttg accaccaggg
     1501 tggccagcgc ctcgccgtcg acgtcctcgg agatgatcac cagcgggcgg cccgcctgga
     1561 cgaccttctc aaggactggc agcagcacct gcgcggagga gaccttctgg ttggcgatca
     1621 ggatgtaggg gtcctcgaag accgcctcca tccgctcggg gtcggtgacg aagtagggcg
     1681 agatgtagcc cttgtcgaac tgcatgccct cg
//
LOCUS       DASWDC010000022         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_672822, whole genome shotgun sequence.
ACCESSION   DASWDC010000022 DASWDC010000000
VERSION     DASWDC010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672822"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VF995_00695"
     CDS             <1..348
                     /locus_tag="VF995_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9342117.1"
                     /translation="IGASEGAKASLIAAPQIRPAVAGVVSLSAERTERGVDVLPWAAR
                     LRSPVLFFTAKDDSFSAEPTPQLYRAATKAASRQLVTLPGSAHGTDLLASPTGPKVQT
                     MILDFLRRYGHAG"
     gene            338..1309
                     /locus_tag="VF995_00700"
     CDS             338..1309
                     /locus_tag="VF995_00700"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9342118.1"
                     /translation="MRVEREQPPVQRRHLRLRPRPDPGHGLRARPGGRVHVATAAVAA
                     VALLGACSSSGSGSGSKSGAGAATTTSAQAQATATTREALKPLVQPSSRCGGPSAKAT
                     LVRFKASDGTSLDGVMVGSGATGVVLVHEYPADLCGFWPFAVYLSHKGIRAFAIDLRC
                     FGLSACPGGDARLRVLDDVAAAVAELHRRGVTRVALAGASMGAAAVLIAGTTVRPPVA
                     AVAELSGEANPTGLVGGIPLDAGAVVKRLDVPSMFVVATNDGYAPVDETRAMYKAAKT
                     HDKQLVVLSGSYDTEHGWNLLSNDPTGVRFTPVAAQVATFLGAHLNG"
     gene            1408..2790
                     /locus_tag="VF995_00705"
     CDS             1408..2790
                     /locus_tag="VF995_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342119.1"
                     /translation="MTSSLSEHVPARDPEDDVDPELRAERGVLAHARRCLEAMRRRAE
                     SLRVAGGDWTSEEALQWSLQQRVASLADDGRTALFFGRVDYDDRVGEGEGAVEDPGLA
                     GARFYLGRRHVIDDQGDPVVIDWRAALAHPFYRASPRRRYGVRRRRRYGWREGVLTGF
                     QDERLDHPEDEEIGARALREEIERPRVGPMRDIVATIQPEQDDLIRAPLSHTICIQGG
                     PGTGKTAVALHRVAFLLYEHRERLERDGVLVVGPSSAYLAFISDVLPGLGEVRVAQVS
                     LEQLVPGVEPGGADPPATATLKGDARMAEVVRRAVFRHLRWPAEPLRLRHGSRAVEVP
                     PGVLRSEIATLLQRGCAGPPAATICAASWSNTATGSWRRPTASPPSTAPATWSTPWPG
                     PGSCAASSTAAGRRWIPPRWSTGCSPTARSWPAPPTGSSTRASGRSSPGGSGRVAAGR
                     GRSGRPRTPS"
BASE COUNT          369 a         1106 c         1007 g          358 t
ORIGIN      
        1 atcggcgcct ccgaaggggc caaggcgtcg ctgatcgccg ccccgcagat ccgcccggcg
       61 gtcgccgggg tggtcagcct ctcggcggaa cgcaccgaac gcggcgtcga cgtgctgccc
      121 tgggccgccc ggctgcgcag cccggtgctg ttcttcaccg ccaaagacga ctcgttctcc
      181 gccgagccga ccccacagct ctaccgggcc gccaccaagg cggcctcgcg gcagctggtc
      241 accctgccag ggtccgccca cggcaccgac ctgctcgcca gcccgaccgg ccccaaggtc
      301 caaaccatga tcctcgattt cctgcggagg tacggccatg cgggttgagc gcgagcagcc
      361 accagtccag cggcgccacc tgcgcctgcg cccgcggcca gacccgggcc atggcctgcg
      421 cgcgcggcct ggcgggcgtg tccacgtggc cacggcggcg gttgcggcgg tggcgctgct
      481 gggcgcgtgt tccagctccg gatcaggctc cggttccaag tccggcgcgg gcgcggccac
      541 cacgaccagc gcccaagccc aggccaccgc cacgacccgg gaggcgctca agccgctggt
      601 ccagccgagc agccgctgcg gcgggccgag cgccaaggcg accctggtgc gcttcaaggc
      661 gagcgacggg accagcctcg acggggtcat ggtcggcagc ggtgcgacgg gggtggtgct
      721 ggtccacgag taccccgccg acctgtgcgg cttctggccg ttcgcggtct acctgagcca
      781 caaggggatc cgcgcgttcg ccatcgacct gcgctgcttc ggcctgtcgg cctgccccgg
      841 cggcgacgcg cgcctgcggg tgctcgacga cgtcgccgcc gccgtcgccg agctgcaccg
      901 ccgcggcgtc acccgggtcg ccctggccgg cgcgtccatg ggcgccgccg ccgtgctgat
      961 cgccgggacc accgtgcgcc caccggtcgc ggcggtggcc gaactgtccg gcgaggccaa
     1021 ccccaccggc ctggtcggcg gcatcccgct cgacgccggc gcggtggtca agcggctcga
     1081 cgtgccgtca atgttcgtgg tcgccaccaa tgacggctac gccccggtcg acgagacccg
     1141 cgccatgtac aaggccgcca agacccacga caagcagctc gtggtgctct ccgggagcta
     1201 cgacaccgag cacggctgga acctgctctc caacgacccc accggcgtgc ggttcacccc
     1261 cgtcgccgcc caggtcgcca ccttcctcgg cgcccacctc aacggctgag gctggaccga
     1321 gaaattccct tgccacaagc ggccggctgg cgcatagtgg tcggccttgt gccgccagcc
     1381 gctggcgctc catccgatga cactccaatg acatcaagcc tgagcgaaca cgtccccgca
     1441 cgggaccccg aagacgacgt cgatcccgag ctccgcgccg agcgcggcgt cttggcgcac
     1501 gcgcggcgct gcctggaggc gatgcggcgc cgcgccgagt cgctgcgggt cgccgggggc
     1561 gactggacct cggaggaggc gctgcagtgg agcctccagc agcgcgtcgc ctcgctcgcc
     1621 gacgacggcc gcaccgcgct gttcttcggc cgcgtcgact acgacgatcg cgtcggcgag
     1681 ggcgagggcg ccgtcgagga ccccggcctg gccggggcgc ggttctacct cggccgccgg
     1741 cacgtgatag acgaccaggg cgacccggtg gtgatcgact ggcgcgccgc cctcgcccat
     1801 cccttctacc gggcaagccc gcgccgccgc tatggcgtgc ggcggcgccg ccgctacggc
     1861 tggcgcgagg gggtcctcac cggcttccag gacgagcgcc tcgaccatcc cgaggacgag
     1921 gagatcggcg cgcgggcgct gcgcgaggag atcgagcggc cccgcgtcgg cccgatgcgc
     1981 gacatcgtcg ccaccatcca gcccgagcag gacgacctga tccgcgcccc gctcagccac
     2041 accatctgca tccagggcgg ccccggcacc ggcaagaccg ccgtggcgct gcaccgggtc
     2101 gcgttcctgc tctacgagca ccgcgagcgg ctggaacgcg acggcgtgct ggtggtcggg
     2161 ccgagcagcg cctacctcgc cttcatcagc gacgtgctgc ccggcctcgg cgaggtccgc
     2221 gtcgcccagg tgtccttgga gcagctcgtg cccggggtcg agccgggcgg cgccgacccg
     2281 cctgcgaccg ccacgctgaa gggcgacgcc cgcatggccg aggtggtgcg ccgcgccgtc
     2341 ttccgccacc tgcgctggcc ggccgagccg ctgcgcctgc gccacggcag ccgcgccgtg
     2401 gaggtcccgc cgggggtgct gcgcagcgag atcgccacgc tgctgcaacg ggggtgcgct
     2461 gggcctccgg ccgcgaccat ctgcgccgct tcctggtcga atactgccac cggcagctgg
     2521 aggcgaccga cggcctcacc gccttcgaca gcgcccgcga cgtggagcac gccctggcca
     2581 ggaccgggga gctgcgccgc ttcctcgacc gcagctggcc ggcgctggat cccgccaagg
     2641 tggtctacgg gctgctcacc gacggcgagg tcctggccgg cgccgccgac gggatcctcg
     2701 acgagggcga gcggcagatc ctcccctggc ggaagcggcc gcgtggcggc tggaaggggg
     2761 cgctctggtc ggccgcggac gccttcctga tcgacgaggc cgccgacgtg atcagtggca
     2821 cccgcaccta cggccacgtg
//
LOCUS       DASWDC010000023         7126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_505915, whole genome shotgun sequence.
ACCESSION   DASWDC010000023 DASWDC010000000
VERSION     DASWDC010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505915"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VF995_00710"
     CDS             <1..1161
                     /locus_tag="VF995_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9342120.1"
                     /translation="SRELPGLDVSLRGAVSIARRLQDPLAELVKIEPKSIGVGQYQHD
                     LSETRLSHSLDAVVEDCVNAVGVDVNTASAPLLARVSGISDGLAAGIVAHRDAAGRFR
                     SRRALKAVPRLGPKAFEQCAGFLRIRGGDDPLDASAVHPEAYPVVRSILTATTGDIGT
                     LIGNHKTLRALRPNDFVTDAFGLPTVTDILAELEKPGRDPRPAFKTATFAEGVETLAD
                     LRAGMLLEGVVTNVAAFGAFVDVGVHQDGLVHISAMSNRFVSDPREIVKPGDVVRVKV
                     LDVDIPRKRIALTLRLDDEAAAAKPAGQRSPQGPRQGQGQRQGQGSRGSGGTPGGGTP
                     GGGAAGGGAAGARPERQGRPRGRGGSPSTPSMPDTALADALRRAGYGKDKKP"
     gene            complement(1186..1890)
                     /locus_tag="VF995_00715"
     CDS             complement(1186..1890)
                     /locus_tag="VF995_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342121.1"
                     /translation="MLEVSALDVFYGDAQALDQLSLQVRAGEIVTLVGSNGAGKTTTL
                     RAICGLLPAARGTLAFEGQPLGGLPGHRRAELGIALVPEGRELWPQLTVRENLELGAW
                     AKAARRHQADSLERVHALFPQLAERSAQVVGSMSGGEQQMVAIGRALMTRPRLLMLDE
                     PSLGLAPVVVRQVFATIRRLHQEGLTILLVEQNVRQALEVADRAYVIETGRVTLQGDA
                     ATLLDNPDVRAAYLGV"
     gene            complement(1883..2656)
                     /locus_tag="VF995_00720"
     CDS             complement(1883..2656)
                     /locus_tag="VF995_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342122.1"
                     /translation="MTGPGSRLLVGEGVCRYFGGLKAVDEVDFHLDEGEILGLIGPNG
                     AGKTTLFAVAAGSIPATRGAVRLGGTPVSGRAAHRSVHLGIARTHQIVRPFAHLTVLE
                     NVLVGWYYGRRRRRPPGGHGGRAAREWAMEILEFIGLADRAQQLPATLTLAGRKRLEV
                     ARALATGPRVLLLDEVVAGLNAVESHRLVALIRQIRAGGVAIIMIEHVMQAVMNVSDR
                     VMVLDHGRKIAEGKPAEVANDPAVIEAYLGHEDDGPRHA"
     gene            complement(2653..3711)
                     /locus_tag="VF995_00725"
     CDS             complement(2653..3711)
                     /locus_tag="VF995_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9342123.1"
                     /translation="MRSGPRIGWRLPAWAPARTLVLFVGALAVLYALPLVVPMGDYAH
                     DLIGLTFMYMAAALAWNWLGGYVGQISFGHAAMFGVGGFVAARIALSTGLPFWAGWLA
                     GGLTAGLYGLLWGHPTLRLRGPYFSIATIGVGEATRLVATYWDPFTGGSSGLSLPLGQ
                     GPTKFALYWYGLYLLAAVVALSWWLRRSRIGLGLLAIKEDVEAAADVGVNATLYQDLV
                     LVLSATVIGVCGGFYAWYQAFIDPQDLFSFDRSIGFVLMGVVGGIGTILGPALGAIVF
                     VVVQELLLASYAQFYLGIYGLLLVLIILFEPLGLSGLLLRLARRLGWRPAAGRAPAIA
                     AGPASSASTTEPGPGGAT"
     gene            complement(3723..4598)
                     /locus_tag="VF995_00730"
     CDS             complement(3723..4598)
                     /locus_tag="VF995_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9342124.1"
                     /translation="MSEVLALAVSAVLLAGFYAAMAYGLGLIYGVLRVVNLAHGGLIM
                     AAAYLTWVLFTRLHIDPYLAIPVVLVVFFAAGVGLYRGLVRLLPRGAEGGVQSLLLLF
                     GVWLILRNLAYLLFSGNDQAVRTSYSTASVRILGNSFAVTRVVVFGVAVIAMLALHLL
                     LRHTLLGKAIRAAGQNPRSCTLVGIDVDRIYALTFGIGVALAGLAGLLASTLFAFNPS
                     YGAGELLRSFVVIVLGGLGSVVGIAAGALVLAFAEVFSIYLVPSYLTAAVGFVLLVVV
                     LVARPAGLFGQRVLG"
     gene            complement(4618..5775)
                     /locus_tag="VF995_00735"
     CDS             complement(4618..5775)
                     /locus_tag="VF995_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9342125.1"
                     /translation="MPDTITVGATLPLTGTESKAGGFYKQGYDLAFDLANRAGGIDVG
                     GKKVKVQLKLLDDTTDQAKAVNLAQRLISQDKVSFMLGTYTTPLVQAQSVVAEQAQIP
                     YVNGGGAALSIYNRGYTWVFGTLASIVNLSTTEMRWLQAQQDAGKLPKPARIAIVWEN
                     TSHGQDFRKGIQDWVKANPGRYTIAVDESFALDGKDFSAVLNKVKAAKADLFMADAHL
                     PDYLTMQRQYISTGMCNKVITYGARGAEPDARQALGTKGVSYIMSAVWWNAQLGDQGL
                     NKTFVDQFKAKYGTDPQWYQAVSYEAARALFAAISQAGSVDKTKVRDALSNLKIPSIL
                     PGGTLSFPAAQGGQASYQFVVQQNLPDGSSPIIYPNDVKKADGIVPNPSCK"
     gene            complement(6162..6896)
                     /locus_tag="VF995_00740"
     CDS             complement(6162..6896)
                     /locus_tag="VF995_00740"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEX9342126.1"
                     /translation="MSRQDLPQPDAAPVAVEVEVEGYLAQVAARLPGPPRAHADIVAE
                     LRSGLLDAADAQRSAGLPPAQAALTAIRESGDPTRVAAGFKAEIAASQARVAATLLVT
                     GPLVGLLWIATAAASHLGLGVHVALPWRWTGRSPGLGVGIPLVAAAVGVTGCAALLGI
                     AATGRLTRWLPARPRRAPTAAAVAGFGAVGADGLGLALLGAQLATVPGKLSPVLAAVA
                     AAASLARLLLAGRAARDCLAIRASLT"
     gene            complement(6893..>7126)
                     /locus_tag="VF995_00745"
     CDS             complement(6893..>7126)
                     /locus_tag="VF995_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9342127.1"
                     /translation="QVDLPTGTVYPALHRLERAGLVQASWQAAGGRRRRVYQLTSAGR
                     RALDAERSTWRDFSAAVTGLLQPAPPAPVAGAP"
BASE COUNT         1078 a         2539 c         2604 g          905 t
ORIGIN      
        1 tcccgtgagc tacctggcct tgacgtgtcg ctgcgtggtg cggtgtcgat tgcccgccgg
       61 ctgcaggacc cgctcgcgga actcgtcaag atcgagccca agtcgatcgg cgtcggtcag
      121 taccagcacg atctgtccga gaccaggctg tcgcactcgc tggacgcggt ggtcgaggac
      181 tgcgtgaatg cggtcggggt cgacgtcaac accgcatcgg cgccgctgct cgcccgcgtc
      241 tcaggcatca gcgacgggct ggcggcggga atcgtcgcgc accgggatgc ggccggaagg
      301 ttccgctctc ggcgggcgct caaggccgtc ccccgcctcg gcccgaaggc gttcgagcag
      361 tgcgcgggct tcctgcgcat ccgcggcggc gacgaccccc tcgacgcctc cgcggtccac
      421 ccggaggcct acccggtggt gcggagcatc ctgacggcga ccaccgggga catcggcacg
      481 ctgatcggca accacaagac gctccgcgcc ctcaggccga acgacttcgt caccgacgcc
      541 ttcggcctgc cgaccgtcac cgacatcctc gccgaactgg agaagccggg ccgcgacccg
      601 cgccccgcct tcaagaccgc caccttcgcc gagggcgtcg agaccctggc ggacctccgc
      661 gcggggatgc tgctggaggg cgtggtcacc aacgtggccg ccttcggcgc gttcgtcgac
      721 gtcggcgtgc accaggacgg cctggtccac atctcggcga tgtccaaccg gttcgtgtcc
      781 gacccccgcg agatcgtcaa gcccggcgac gtggtgcggg tgaaggtcct ggacgtcgac
      841 atcccccgca agcgcatcgc cctcaccctc cgcctggacg acgaggccgc cgctgccaaa
      901 cccgccggcc agcgatcgcc gcagggaccg cggcaggggc agggacagcg gcagggacag
      961 gggtcccgag gcagcggcgg cacgcctggc ggcggcacgc ctggcggcgg tgcggcgggc
     1021 ggcggtgcgg cgggcgcacg gccagagcgg caggggcgac cacggggccg gggcggctcg
     1081 ccctccacgc cctccatgcc ggacacggcg ctcgccgacg cgctgcgccg cgccggctac
     1141 ggcaaggaca agaagccctg atcgccctcg acctgtaccg cgggttcaca ccccgaggta
     1201 ggcggcgcgg acgtcgggat tgtcgagcag cgtggcggcg tcaccctgga gggtgacgcg
     1261 gccggtctcg atcacatagg cgcggtcggc gacctccagc gcctgccgga cgttctgctc
     1321 gaccagcagg atggtcaggc cttcctggtg gaggcggcgg atggtggcga acacctggcg
     1381 gacgacgacc ggggccaggc cgaggctcgg ttcgtccagc atgagcaggc gcgggcgggt
     1441 catcagcgcc cggccgatgg cgaccatctg ctgctcgccg ccggacatgc tgccgaccac
     1501 ctgggccgag cgctccgcga gctgggggaa cagcgcgtgg acgcgctcca gcgagtcggc
     1561 ctggtggcgg cgggccgctt tggcccaggc gccgagctcc aggttctcgc ggacggtgag
     1621 ctgcggccac agctcccggc cctcgggcac cagggcgatg cccagctccg cgcggcggtg
     1681 gccgggcagg ccgccgaggg gctggccctc gaaggcgagg gtcccccgcg ccgccgggag
     1741 cagcccgcag atcgcccgca gggtggtggt cttgccggcg ccgttggagc cgaccagggt
     1801 cacgatctcc ccggcgcgga cctggaggct gagctggtcg agcgcctggg cgtcgccgta
     1861 gaagacgtcg agggcgctga cctcaagcat ggcggggtcc gtcgtcctcg tggccgaggt
     1921 aggcctcgat cacggcgggg tcgttggcga cctcggcggg cttgccctcg gcgatcttgc
     1981 ggccgtggtc gaggaccatg acgcggtcgg acacgttcat caccgcctgc atcacgtgct
     2041 cgatcatgat gatggcgacg ccgcctgccc ggatctgccg gatcaatgcc accagccggt
     2101 ggctctcaac ggcgttgagg ccggcgacga cctcgtcgag cagcagcacc cggggaccgg
     2161 tggcgagcgc gcgggccacc tccagccgct tgcgccccgc gagcgtgagc gtggcgggga
     2221 gctgctgtgc ccggtccgcc aggccaatga actcgaggat ctccatcgcc cactcccgcg
     2281 cggcgcgccc gccgtggccg ccgggcggcc tccgcctccg gcggccgtaa taccagccga
     2341 ccaggacgtt ctcgagcacg gtcaggtgcg cgaagggccg cacgatctga tgggtgcggg
     2401 cgatgccgag gtggaccgag cggtgtgcgg cccggccgct gaccggcgtg ccgccgagcc
     2461 gcaccgcgcc ccgggtcgcg gggatggacc cggcggcgac cgcgaacagg gtggtcttgc
     2521 cggcgccgtt gggcccgatg aggccgagga tctcgccctc gtccaggtgg aagtccacct
     2581 cgtcgaccgc cttgaggccg ccgaagtagc ggcacacccc ttcgccgacc agcagccggg
     2641 acccgggccc ggtcatgtcg cgccgcccgg tccaggttcg gtggtcgatg ccgacgacgc
     2701 cgggccggcg gcgatagctg gcgcccggcc ggcggcgggc cgccagccga ggcggcgggc
     2761 gagccgcagc agcagccccg acaggcccag cggctcgaac aggatgatca gcaccagcag
     2821 cagcccgtag atgccgaggt agaactgggc gtaggaggcg agcagcagct cctgcaccac
     2881 cacgaacacg atcgcgccga gggcggggcc gaggatggtg ccgatgccgc cgacgacgcc
     2941 catcagcacg aagccgatcg agcggtcgaa gctgaacagg tcctgcggat cgatgaatgc
     3001 ctggtaccag gcgtagaacc cgccgcagac accgatcacc gtggcggaga ggaccagcac
     3061 caggtcctgg tacagggtgg cgttgacgcc gacgtcggcg gccgcctcga cgtcctcctt
     3121 gatggcgagc aggccgagcc cgatgcggga gcggcgcagc caccacgaca gcgccaccac
     3181 cgccgccagc agatacaggc cataccagta cagcgcgaat ttcgtcggtc cctgcccgag
     3241 cggcagggac aggccggagg acccgccggt gaacgggtcc cagtaggtcg ccaccaggcg
     3301 ggtggcctcg ccgacgccga tggtggcgat ggagaagtag gggccgcgca gccgcagcgt
     3361 cgggtgcccc cacaacaggc cgtagaggcc ggcggtcagc ccgccggcca gccagccggc
     3421 ccagaacggc aggccggtgg agagcgcgat ccgcgccgcg acgaacccgc cgacgccgaa
     3481 catggcggcg tggccgaagg agatctgccc gacgtagccg ccgagccagt tccacgccag
     3541 cgccgccgcc atgtacatga aggtgaggcc gatgaggtca tgggcgtagt cccccatcgg
     3601 caccaccagc ggcagcgcgt acagcaccgc cagggcgccc acgaagagga cgagggtgcg
     3661 ggcgggcgcc catgcgggca gcctccaccc gatccgcggt ccgctcctca ccagccgcgc
     3721 cctcatccca gcacccgctg cccgaacagc ccggcgggac gtgccaccag cacgaccacc
     3781 agcagcacga agccgaccgc cgcagtgagg taggacggca ccaggtagat gctgaacacc
     3841 tcggcgaacg ccagcaccag cgcgccggcg gcgatgccga cgacgctgcc gaggccgccg
     3901 agcacgatca ccacgaagct tcggagcagc tcccccgccc cgtaggaggg gttgaaggcg
     3961 aacagggtcg aggcgagcag cccggcgagc ccggccagcg cgacgccgat cccgaaggtc
     4021 agcgcgtaga tccggtccac gtcgatgccg accagggtgc agctccgcgg gttctgccca
     4081 gcggcccgga tcgccttgcc gagcagggtg tgccgcagca gcaggtgcag cgccagcatg
     4141 gcgatgaccg cgacgccgaa gaccaccacc cgggtcaccg cgaaggagtt gcccaggatc
     4201 cgcacggacg ccgtggagta cgacgtccgc acggcctggt cgttgccgga gaacagcagg
     4261 taggcgagat tccgcaggat cagccacacg ccgaacagca gcagcagcga ctgcacgccg
     4321 ccctccgcgc cgcgtggcag caggcgcacc agcccccggt agagcccgac cccggcggcg
     4381 aagaacacca ccagcaccac cgggatggcc aggtagggat cgatgtggag gcgggtgaac
     4441 agcacccagg tgaggtaggc ggccgccatg atcaggccgc catgggccag gttgaccacc
     4501 cgcagcacgc catagatcag gccgaggccg taggccatgg cggcgtagaa ccccgccagg
     4561 agcaccgccg agaccgccag cgcgaggacc tcactcacca gggctccagg ggttcagtta
     4621 tttgcacgac gggttgggca cgatcccgtc ggccttcttc acgtcgttgg ggtagatgat
     4681 cggcgaggag ccgtccggca ggttctgctg gacgacgaac tggtaggagg cctggccgcc
     4741 ctgggctgcc gggaacgaca gcgtcccgcc gggcaggatc gaggggatct tgaggttcga
     4801 cagcgcgtcg cggaccttgg tcttgtccac cgagccggcc tgcgagatcg ccgcgaacag
     4861 cgcccgcgcc gcctcgtagg agaccgcctg gtaccactgc gggtcggtgc cgtacttcgc
     4921 cttgaactga tcgacgaagg tcttgttcag gccctggtcg ccgagctggg cgttccacca
     4981 caccgccgac atgatgtagc tgaccccttt ggtgccgagc gcctggcgcg cgtcgggctc
     5041 ggcgccgcgg gcgccgtagg tgatcacctt gttgcacatc cccgtcgaga tgtactggcg
     5101 ctgcatggtg aggtagtccg gcaggtgcgc gtcggccatg aacaggtccg ccttggcggc
     5161 cttcaccttg ttgagcacgg cggagaagtc cttgccgtcg agcgcgaagg attcgtccac
     5221 cgcgatggtg tagcggccgg ggttggcctt gacccaatcc tggatgccct tgcggaagtc
     5281 ctggccgtgg gaggtgttct cccacacgat cgcgatcctg gctggcttgg ggagcttgcc
     5341 ggcgtcctgc tgggcctgca gccaccgcat ctcggtggtg ctgaggttga cgatcgaggc
     5401 cagggtgccg aacacccacg tgtagccccg gttgtagatc gagagcgcgg ccccgccgcc
     5461 gttgacgtag gggatctggg cctgctcggc gacgacgctc tgcgcctgga ccaacggcgt
     5521 ggtgtaggtg ccgagcatga agctgacctt gtcctgcgag atcagccgct gcgccaggtt
     5581 caccgccttg gcctggtcgg tggtgtcgtc gagcagcttc agctgcacct tcaccttctt
     5641 gccgccgacg tcgatcccgc cggcgcggtt ggcgaggtcg aaggcgaggt cgtagccctg
     5701 cttgtagaag ccgcccgctt tggattcggt tccagtcagc ggcagggtcg cgccgaccgt
     5761 aatggtgtcg ggcaccgtga cggcaccggc agcctgcttg ctgtgctgcg ccggagcgca
     5821 ccccacagcc accagcgccc ccacaatggg aagcgccacg aaccaccagc ggcatcgccg
     5881 gatccgggaa tctctcaccg cagtcccctc cagtcatgcc cagatggagg cagatggaca
     5941 ggtacggtga gcggcggcgc cctcgccgtg ctcggcctac cgcgacggag gacgccatgc
     6001 ggcggcacca tcacgctggt cgccccggtc gccatcgccg ccgccatcgg ccactccagt
     6061 aggctcggat cgtataccgc atacgcccat tgatgggtag cttcccggag aacgcgccgc
     6121 ctgtccagtt cccgccctgg cttccttcgc cggtgtcgcg gtcaggtcag gctcgcgcgg
     6181 atggccaggc aatcgcgtgc ggcgcgcccg gcgagcagca gccgggcgag gctggccgct
     6241 gccgccacgg cggccaggac tggggagagc ttccccggca cggtggccag ctgggcccca
     6301 agcagcgcga ggccgagccc gtcggcgccc actgcgccga acccggcgac cgccgcggcg
     6361 gtcggggccc ggcgcggcct ggcgggcagc cagcgggtga gccggccggt ggcggcgata
     6421 ccgagcagtg ccgcgcaccc agtgactccg acagcggcgg cgacgagggg gatgcccacg
     6481 ccaaggccgg gtgaccggcc ggtccaccgc cagggcaggg cgacatggac gccgaggccg
     6541 aggtggctgg cggcggccgt ggcgatccac agcaggccga ccagcgggcc ggtcaccagc
     6601 agggtggccg cgacgcgtgc ctggctggcg gcgatctcgg ccttgaaccc ggcggcgacc
     6661 cgcgtcgggt cgcccgactc gcggattgcc gtcagggcgg cctgggccgg cggcagcccg
     6721 gcggaccgct gcgcatccgc cgcgtccagg agcccggagc gcagctcggc cacgatgtcg
     6781 gcatgggcgc ggggcgggcc gggcagccgc gcggcgacct gggcgagata accctcgacc
     6841 tcgacctcga ccgccaccgg cgcggcgtct ggttgcggca ggtcctgcct gctcatggcg
     6901 cgcccgccac tggcgctggc ggcgcgggtt gcaggaggcc ggtgacggcg gcggagaagt
     6961 cccgccaggt gctgcgttcg gcgtccaggg cccgccggcc cgccgaggtc aactggtaga
     7021 cgcggcggcg gcgcccgccg gccgcctgcc agctcgcctg caccaggccc gcccgttcca
     7081 ggcggtgcaa ggccggatac accgtgccgg tcggcaggtc gacctg
//
LOCUS       DASWDC010000024         3520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1341076, whole genome shotgun sequence.
ACCESSION   DASWDC010000024 DASWDC010000000
VERSION     DASWDC010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341076"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VF995_00750"
     CDS             complement(<1..1029)
                     /locus_tag="VF995_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9342128.1"
                     /translation="MSVAGSSSRSTWRPIGRLAATLTVLMLVAAGCGLKSSKSSSSGG
                     SSTSSGTIKLGILTDCEGAFGSFNEQTIGGALAAFAAYAGGKVTNPNKPSQGMSGITV
                     AGRPIKIVGFGCSNDRADTAINETKRLMEQLGADILLGPLSGDEAIAVANYAKAHPDK
                     TFINGTAGSFDPTMLVRAPNFFRFNGDGAQWNAGLGDLAFNTLGWKRVAVIGDDYSFA
                     WTSMAGFIADYCGAGGNVVKRVFPPLNTTDYSSWVRQLPPPSQVDGYFWAVGGAGLIP
                     ALKAFEQAYGPINPKQHMGNLFWGTPGQFQQLSKRVAGVYVGSAGTAGDLKTPQATTY
                     TANIGKAFQ"
     gene            complement(1010..2077)
                     /locus_tag="VF995_00755"
     CDS             complement(1010..2077)
                     /locus_tag="VF995_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9342129.1"
                     /translation="MLAVALLGAPILYTRYVTTEILTRSLILGLVAATVAFLYSYAGV
                     LSLAQIGVFGTAGFLYGNFVTHGETKGLNLGWNPWLAFLVAVLAATAVAFLFGLVASR
                     SAGIYYMMITLVFAVIVNYFFGQVTTLSGFGGISNIDSYVPWPVGSPDQHPFRLYYAI
                     LLLVALAYTAMRRVLRTPFGLTAQGIRDDPVRMASLGYNVPLHRALVFTFTGLIAAVA
                     GVMFTWWNGHIDPATVGLGASLDLIVVAVIGGISQLSGAFLGALVFVAVDNYVRDIAS
                     LSDRFHTVIGLIFLLIVVASPGGLLGLWASLWERRPAPLRRTGTGTGTGNGTGTPVDP
                     RHRPEGGSREGSEAEHVRGRQ"
     gene            complement(2161..3231)
                     /locus_tag="VF995_00760"
     CDS             complement(2161..3231)
                     /locus_tag="VF995_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9342130.1"
                     /translation="MTAASATEVEGVAVRRRAVRPVVLASALLAAFALVWILFLSSQS
                     AVRQPAQFIAALLDGLTLAGLYFLVASGFTLVFGLMRVVNLAHGSFFLLGGYIALQLQ
                     REWVGQGGGFGLTSADVDLFGGWLLPLVVATVLVAGIGLIVQQLLLRWNQGDELRQVL
                     ITIALSIIAADQMLAHFGGIAEDVAWPRQLDHSVTIPLTHLRYSTTRLFILALAIVVG
                     VALWLWLTRTRMGAIIRAGVDDRQMVSALGINIQLAFALAFLVGAALAAMDGVVGGSF
                     ASLAPGVDAQWLLNSLVVVVIGGMGSLPGAALGALLYGLAGSLAATYLPAGYSYYSVI
                     VTFVLLALVLAARPYGIFGRPA"
     gene            complement(3249..>3520)
                     /locus_tag="VF995_00765"
     CDS             complement(3249..>3520)
                     /locus_tag="VF995_00765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017815147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX9342131.1"
                     /translation="MRMNAFHGTRLDTLLRGFGTETLIITGAWTNMSIEHTARHAADA
                     GYEVVVCSDGTSTISEEWHHAGLNYALTNVGTVATCAEVAKALGA"
BASE COUNT          575 a         1180 c         1293 g          472 t
ORIGIN      
        1 ctggaacgcc ttgccgatgt tggcggtgta ggtggtcgcc tgcggggtct tcaggtcccc
       61 ggcggtgccg gcggagccga cgtagacccc ggcgacgcgc ttggagagct gctggaactg
      121 cccgggggtg ccccagaaca ggttgcccat gtgctgcttg gggttgatcg ggccgtaggc
      181 ctgctcgaac gccttcagcg cggggatcag cccggcgccg ccgaccgccc agaagtaccc
      241 gtcgacctgg ctcggcggcg gcagctggcg cacccaggag gagtagtcgg tggtgttgag
      301 cggcgggaag acccgcttga ccacgttgcc gccggcgccg cagtagtcgg cgatgaagcc
      361 ggccatggag gtccaggcga agctgtagtc gtcgccgatg accgcgaccc tcttccagcc
      421 gagcgtgttg aacgccaggt cgccgaggcc ggcgttccac tgggcgccgt cgccgttgaa
      481 gcggaagaag ttcggcgccc gcaccagcat ggtcgggtcg aacgacccgg cggtgccgtt
      541 gatgaaggtc ttgtccgggt gcgccttggc gtagttggcc acggcgatcg cctcgtcacc
      601 cgacagcggg ccgagcagga tgtcggcgcc gagctgctcc atcagccgct tggtctcgtt
      661 gatggcggtg tcggcgcggt cgttggagca gccgaagccg acgatcttga tcgggcggcc
      721 ggccacggtg atgccgctca tcccctgtga cggcttgttc gggttggtca ccttgccgcc
      781 cgcgtaggcg gcgaaggcgg ccagggcgcc gccgatggtc tgttcgttga agctaccgaa
      841 ggcgccttcg cagtcggtca ggatgccgag cttgatggtg cctgacgacg tcgaggagcc
      901 gccgctggag ctgctcttgc tgctcttgag gccgcacccg gcggcgacca gcatcaggac
      961 ggtgagcgtc gcggccaggc ggccgatcgg ccgccaggtt gacctggaac tactgcctgc
     1021 cacggacatg ctcggcctcg cttccctctc gtgaccctcc ctcgggtcgg tggcgcggat
     1081 cgacgggtgt gccagtgcca ttgcctgtgc cagttccggt tccagtgcgg cggagggggg
     1141 cgggccggcg ctcccagagg gacgcccaga gcccgaggag gccgccgggg gaggcgacca
     1201 cgatcagcag gaagatcaac ccgatcacgg tgtggaagcg gtcgctgagc gaggcgatgt
     1261 cgcgcacgta gttgtccacg gcgacgaaca ccagcgcgcc gaggaaggcg ccggagagct
     1321 gcgagatgcc gccgatcacc gccaccacga tcaggtcgag ggaggcgccg agcccgacgg
     1381 tggccgggtc gatatggccg ttccaccagg tgaacatgac cccggcgacg gcggcgatca
     1441 gcccggtgaa ggtgaacacc agcgcccggt gcagcggcac gttgtagccg agcgaggcca
     1501 tccgtaccgg gtcgtcgcgg atcccctgcg cggtcagccc gaagggcgtc cgcagcaccc
     1561 ttcgcatggc ggtgtaggcg agcgcgacca gcagcaggat cgcgtagtac agccggaagg
     1621 ggtgctggtc ggggctgccg acgggccagg gcacgtagga gtcaatgttg gagatgccgc
     1681 cgaagccgga cagggtggtg acctgcccga agaagtagtt cacgatcacc gcgaacacca
     1741 gcgtgatcat catgtagtag atgccggcgc tcctggaggc gaccagcccg aacaggaacg
     1801 cgaccgcggt cgccgccagc accgccacca ggaacgccag ccacgggttc cagccgaggt
     1861 tgagcccctt ggtctcgccg tgggtgacga agttgccgta caggaacccg gcggtgccga
     1921 acacgccgat ctgcgcgagc gacagcaccc cggcgtagga gtagaggaac gccaccgtgg
     1981 ccgcgaccag cccaaggatc aggctgcggg tgaggatctc ggtggtgacg tagcgggtgt
     2041 agaggatcgg cgcgcccagc agcgcgacgg ccagcacgat cgcccgccac cccgggcgcc
     2101 gcggcctcca cccgcgaaca ctcgcggcgc tcgcggtcat tggcgcaccg cccccggcgc
     2161 tcatgccggc cgtccgaaga tcccgtaggg ccgtgcggcg agcaccagcg ccagcagcac
     2221 gaaggtgacg atcaccgagt agtagctgta cccggccggc aggtaggtgg cggccagcga
     2281 ccccgccagc ccgtacagca gcgcgccgag ggccgcgccc ggcagcgacc ccatcccgcc
     2341 gatcaccacc acgaccaggg agttcagcag ccactgggcg tccacgcccg gggcgaggct
     2401 ggcgaaggag ccgcccacca ccccgtccat ggcggcgagc gcggcgccga ccaggaaggc
     2461 gagcgcgaag gcgagctgga tgttgatccc gagcgcggaa accatctgcc ggtcgtcgac
     2521 cccggcgcgg atgatcgcgc ccatgcgggt gcgggtgagc cacagccaca gcgccacgcc
     2581 gaccacgatc gccagcgcca ggatgaacag ccgggtggtc gagtagcgca ggtgggtcag
     2641 cgggatggtg acgctgtggt cgagctggcg cggccaggcg acgtcctcgg cgatcccgcc
     2701 gaagtgggcc agcatctggt cggcggcgat gatcgacagg gcgatggtga tcagcacctg
     2761 ccgcagctcg tcgccctggt tccagcgcag cagcagttgc tgcacgatca gcccgatccc
     2821 cgcgacgagc acggtggcga ccaccagcgg cagcagccac ccgccgaaga ggtccacgtc
     2881 ggcgctggtg agcccgaagc ctcccccctg ccccacccat tcgcgctgca gctgcagggc
     2941 gatgtagccg ccgagcagga agaagctgcc gtgggccagg ttgaccaccc gcatcagccc
     3001 gaacaccagc gtgaacccgc tggcgaccag gaagtacagc ccggcgaggg tgaggccgtc
     3061 cagcagcgcg gcgatgaact gggcgggctg cctgaccgcc gactggctgg agaggaacag
     3121 gatccagacc agcgcgaacg cggccagcag cgcgctcgcc agcacgaccg gccgcacggc
     3181 gcggcgccgg acggcgacgc cctcgacctc ggtggcggag gcagcggtca cggcagggtc
     3241 cctccgcctc aggcgccgag cgccttggcc acctcggcgc aggtggcgac ggtgccgacg
     3301 ttggtcaagg cgtagttgag gccggcatgg tgccactcct cgctgatcgt ggaggtgccg
     3361 tcggagcaga ccaccacctc gtagccagcg tcggcggcgt gccgggcggt gtgctcgatc
     3421 gacatgttgg tccacgcgcc ggtgatgatc agcgtctcgg tgccgaagcc gcgcagcagg
     3481 gtgtccagcc gggtgccgtg gaaggcgttc atgcgcatct
//
LOCUS       DASWDC010000025         4790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_921162, whole genome shotgun sequence.
ACCESSION   DASWDC010000025 DASWDC010000000
VERSION     DASWDC010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921162"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1268
                     /locus_tag="VF995_00770"
     CDS             216..1268
                     /locus_tag="VF995_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX9342132.1"
                     /translation="MKDVGIVGLPLSGKTTLYNALTRAGIPTGGAVGKASVAAVPVPD
                     PRVDVLAELERSRKRVHAQVRFVDVAGLSAAGASGKGGLSAQGLGALREVDALALVLA
                     AFDGGTEPVEAAADLSLELTVADLVSVESGAEKAAKRAKAGVKGAASEAETLSRAREL
                     LESGTPLRAAGWEPEQLKLLRNFSPLTLKPALAVVNLGEDQIGEAAARAATVEEALDV
                     PAVALPAALEAEAAELAPEESAAFLAEYGIERGGLDQVIGAAWRLLDLETFFTTGEDE
                     TRAWEVHRGATAPEAAGRIHSDLERGFIRAEVISYDDLVAAGGWDAARAKGLLHSEGK
                     SYVVGEGDCLHIRFNV"
     gene            complement(1295..2323)
                     /locus_tag="VF995_00775"
     CDS             complement(1295..2323)
                     /locus_tag="VF995_00775"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX9342133.1"
                     /translation="MSTDTTDQIPGPGPGNPVPDFRAPARVLLAAPRGYCAGVDRAIE
                     IVEVALQTYGPPVYVRKQIVHNHHVVRRLEELGAVFVEELGEVPQGATVVFSAHGVAP
                     SVHAEARERHLRVIDATCPLVTKVHQEARKFAAQGYDIVLIGHEGHEEVAGTMGQAAA
                     AIHLVSDEDEAGRVRIPDPRRVAYLTQTTLSVDETVGVVDRLRERFPALAGPRSDDIC
                     YATQNRQAAVKALAADCELILVIGSDNSSNSQRLVEVARGLGCQSHLIPDAAALDPSW
                     FEGVATVGVTSGASAPEWLVADLVEALRERGAREVREVPVVPEDVHFALPRTLQQDAR
                     GQDAQRGG"
     gene            2606..3808
                     /locus_tag="VF995_00780"
     CDS             2606..3808
                     /locus_tag="VF995_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342134.1"
                     /translation="MAPAPPPQFGTSIRRGARSRWTWAAIGLIVLLLAALSQLPFSPH
                     ARADVVPTPDITAHTNGRVAAITKLGNVIYIGGTFTTVTTLGGTEVAMPYLAAVDATT
                     GDLITSFAPSPNAQVRALANDGTKIFAAGDFTSIGGAARTKLAAVNASTGAADATFIA
                     HPAGGGDTIYALMSSTLHVFVGGSFSQIVDGGGPHSLPRFAMLSASTGTVTSWRPAVN
                     NAVYAISFSSSRSRVFLGGKFTTAGGAAHNGLVSLVASSGAVDSAFTANSVYPVLSLA
                     VSPNRVYPGIAGSSGGRCAGFNATSGARAFWIVTNGNVQATSYQNGILYCGGHFSGAG
                     SFGGLQRAKEGAVNTTTGAVTDYAPAFNSPLGIFAQGFSGTKLLTGGDFTTVSGVGQQ
                     GYAQVPTS"
     gene            complement(3823..>4790)
                     /locus_tag="VF995_00785"
     CDS             complement(3823..>4790)
                     /locus_tag="VF995_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9342135.1"
                     /translation="AAAAQFGWRPVLPLFAALAGVLIPPLAPCMRALWSTLLGRGPAL
                     QAAFSLEAAGQEVNFIVGPLLVVALATALSPAVAMLAIGLLAFGGTLWFATSPASRAW
                     RGSGSRRSSHWAGALGVAGIRTLVTAALAFAVGIGAVEVAVPAVSEHFGSRALAGPFL
                     AIWSLGSMIGGVLAGARKPSGAPERRLWLLFGLIAVDFVPLTLARSPLPFGAAMALAG
                     LAIAPGIACLYLLVDRAAPDGTLTEAFTWVTCAFTAGIALGSAVGGALVQHVGTGAAF
                     LLGAVTAGAAAVIAWSRHASLRATAAHPPEPGAGSELEPEPRPAG"
BASE COUNT          707 a         1778 c         1685 g          620 t
ORIGIN      
        1 agtctcaccc ctgacccact cgacacccct gaccccactc gacacctttc ctaggggtaa
       61 gctgcgagcc tgcgccgccg gatccaccgt aggcccggcg gggtcccgtt ctcgcgacct
      121 gctgttcgga gcggagaacg ggaccccgcc gggccatccc gccgcggcgc agtcccgcac
      181 ccggggtatc cctaccaacc caacgagagg cgctcatgaa agacgtcggc atcgtcggct
      241 tgccgctttc aggaaagaca acgctgtaca acgcgctcac cagggcaggt atcccgacag
      301 gcggggcagt tgggaaggcg agcgtggccg cggtgccggt gccggacccg cgggtggacg
      361 tgctggctga gctggaacgc tcgcgtaagc gggttcacgc gcaggtccgc ttcgtggacg
      421 tggcggggct ctccgctgcc ggggcgtccg gcaagggcgg cctgtcggcg caggggcttg
      481 gggcgctgcg ggaggtcgac gcgctggcgc tggtgctggc cgctttcgac ggcggcaccg
      541 aaccggtcga ggccgccgcc gacctcagcc tggagctgac cgtggcggac ctcgtcagcg
      601 tcgaatccgg ggccgagaaa gccgccaagc gcgccaaggc cggcgtcaaa ggtgccgcgt
      661 ccgaagcgga gaccctgtcg agggcgcggg agctgctcga atccggcacg ccgctgcgcg
      721 cggccggctg ggaacccgag cagctgaagc tgctgcgcaa cttctcgccg ctgacgctca
      781 agccggcgct ggcggtggtg aacctcggcg aggaccagat cggcgaggcc gccgcgcggg
      841 ccgccacggt cgaggaggcg ctcgacgtgc ccgccgtggc gttgccggcc gcgctggagg
      901 cggaggcggc cgagttggca ccggaggagt cggccgcctt cctggcggag tacgggatcg
      961 agcggggcgg cctcgaccag gtgatcggcg cggcctggcg gctgctggac ctggagacct
     1021 tcttcaccac cggcgaggat gagacccgcg cctgggaggt ccaccgcggg gcgaccgcgc
     1081 cggaggccgc cgggcggatc cactccgacc tcgaacgcgg cttcatccgc gccgaggtga
     1141 tctcctacga cgacctggtc gcggccggcg gctgggacgc ggcccgcgcc aagggcctgc
     1201 tccacagcga gggcaagtcc tacgtcgtcg gcgagggcga ctgcctgcac atccgcttca
     1261 acgtctgacc acccggcgcc agggcctcag cggctcagcc gccgcgttgg gcgtcctggc
     1321 cccgggcgtc ctgctggagg gtgcggggta gcgcgaaatg gacgtcttcg gggaccacgg
     1381 ggacctcgcg gacctcgcgg gcgccgcgct cccggagcgc ctcgaccagg tcggcgacca
     1441 gccattcggg cgctgaggcg ccggaggtga ccccgacggt ggcgacgccc tcgaaccagg
     1501 acgggtcgag cgcggcggcg tcggggatga ggtggctctg gcagccgagg ccgcgggcga
     1561 cctcgaccag ccgctgggag ttcgaggagt tgtcggagcc gatcaccagg atcagctcgc
     1621 agtcggcggc cagcgccttg acggccgcct ggcggttctg ggtggcgtag cagatgtcgt
     1681 ccgagcgcgg cccggcgagc gccgggaagc gctcgcggag gcggtccacc acgccgacgg
     1741 tctcgtcgac cgacagggtg gtctgggtca ggtaggcgac ccgccgcggg tcggggatcc
     1801 ggacgcggcc tgcctcgtcc tcgtctgaga ccaggtggat ggcggcggct gcctggccca
     1861 tggtgccggc gacctcttcg tggccctcgt gcccgatgag gacgatgtcg tagccctgcg
     1921 cggcgaactt gcgcgcctcc tggtggacct tggtgaccag cgggcaggtg gcgtcgatca
     1981 cccgcagatg gcgctccctg gcctccgcgt gcaccgaggg cgccacgcca tgggcggaga
     2041 acaccacggt cgcgccctgt ggcacctcgc cgagctcctc cacgaagacg gcgcccagct
     2101 cctccagccg ccggacgacg tggtggttgt ggacgatctg cttgcggacg tagaccggcg
     2161 ggccgtaggt ctggagggcg acctcgacga tctcgatggc gcggtccacg ccggcgcagt
     2221 agccgcgtgg cgcggccagc agcacccgtg ccggagcccg gaagtcaggg accgggttgc
     2281 cggggccggg gccggggatc tggtcggtgg tgtcggtgga catgccccat tctacggatg
     2341 ggccagccca atgggcttgc gccagccggc atctcccaaa atccctcatc tatttcatat
     2401 ctttcatagc cgccgtttct cgcgttcctc gctggatcaa gcgatccaag cgcaaccgct
     2461 tctcggcgca gtagacgaac tcgcgcattt gcacgatgcg gcggagtccg tttcagcgtg
     2521 aaatgacgtt cgcttccgag ccggacccca ctccggcccg gtttttccct atggcccccc
     2581 aacccaaaag gaagcgaacg gcttgatggc tccagcccct ccgcctcagt tcggcacgag
     2641 catccggcgc ggcgcccggt cccgttggac ctgggcggcg atcgggctca tcgtgctgct
     2701 gctcgccgcg ctgagccagc tccccttctc ccctcacgct cgcgcggacg tggtgccgac
     2761 cccggacatc accgcccaca ccaacgggcg ggtcgcggcg atcaccaagc tcggcaatgt
     2821 catctacatc ggcggcacct tcaccaccgt caccaccctc gggggcaccg aggtcgccat
     2881 gccgtacctg gccgccgtcg acgcgaccac cggcgacctg atcaccagct tcgcaccgag
     2941 ccccaacgcc caggtccggg cgctggccaa tgacggcacc aagatcttcg cggcgggcga
     3001 cttcaccagc atcggtggtg ccgcccgcac caagctggcc gccgtcaacg ccagcaccgg
     3061 cgccgccgac gccaccttca tcgcccaccc ggccggcggc ggcgacacca tctacgcgct
     3121 gatgtcgagc accctgcatg tgttcgtggg cggcagcttc tcccagatcg tcgacggcgg
     3181 cggcccccac agcctgcctc gcttcgcgat gctttcggcc tccaccggga ccgtgaccag
     3241 ctggcggccg gcggtgaaca acgccgtcta cgcgatcagc ttctcctcca gccgctcccg
     3301 ggtgttcctc ggcggcaagt tcacgaccgc cggaggtgcc gcccacaacg gactcgtctc
     3361 gctggtcgcc tcgagcggcg ctgtggacag cgccttcacg gccaacagcg tctacccggt
     3421 cctcagcctg gcggtcagcc ccaaccgcgt gtacccggga atcgccggct ccagcggggg
     3481 acgctgcgcc gggttcaacg ccaccagcgg cgccagggcc ttctggatcg tgaccaacgg
     3541 caacgtccag gccaccagct accagaacgg catcctctac tgcggcggcc acttctccgg
     3601 cgccggctcg ttcggcggcc tccagcgggc caaggaaggc gccgtcaaca ccaccaccgg
     3661 cgcggtcacc gactacgccc ccgccttcaa cagcccgctc ggcatcttcg cgcaggggtt
     3721 cagcggcacc aagctgctca ccggcggcga cttcaccacc gtctccgggg tcggccagca
     3781 gggatacgcg caggtcccga cctcctgaga cccactcctc gcctaccccg ccggtctcgg
     3841 ctccggctcc agttctgagc ccgcgccggg ctccggcggg tgggcggcgg tggcgcgcag
     3901 cgaggcgtgc cgggaccagg cgatcacggc ggcggccccg gcggtgaccg cgccgagcag
     3961 gaacgccgcg ccggtgccga catgctggac aagcgcgccg ccaacggcgc tgccgagcgc
     4021 gatcccagcg gtgaacgcgc aggtcaccca ggtgaacgcc tcggtaaggg tgccgtcagg
     4081 ggcggcccgg tcgaccagca ggtacaggca ggcgatccct ggggcgatcg ccaggcccgc
     4141 cagcgccatc gcggccccga acgggagtgg cgagcgggcc agggtcagcg gcacgaagtc
     4201 gaccgcgatc aagccgaaca gcagccacag ccgccgctcg ggtgcgccgg acggcttgcg
     4261 ggcgccggcc agcacgccgc cgatcatgct gcccaaactc cagatcgcca ggaacgggcc
     4321 ggccagggcg cggctgccga agtgctcgga gaccgccggg accgccacct cgaccgcgcc
     4381 gatgccgacc gcgaaggcca acgccgccgt caccagcgtg cggatccccg cgacgccaag
     4441 cgcgccggcc cagtggctgg agcggcggct cccgctgccc cgccaggcac gggacgccgg
     4501 gctggtcgcg aaccagagcg tgccgccgaa ggcgagcagc ccgatcgcca gcatcgcgac
     4561 cgccggggag agggcggtgg ccagcgcgac caccagcagc ggcccgacga tgaagttgac
     4621 ctcctgcccc gccgcctcca gcgagaaagc cgcctgcagc gccggcccgc gcccgagcag
     4681 agtcgaccac agcgcccgca tgcacggcgc cagcggcggg atcagcaccc ccgccagcgc
     4741 cgcgaacagc ggcaacaccg gccgccagcc gaactgggcc gcggcggcca
//
LOCUS       DASWDC010000026         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_256774, whole genome shotgun sequence.
ACCESSION   DASWDC010000026 DASWDC010000000
VERSION     DASWDC010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256774"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /gene="purM"
                     /locus_tag="VF995_00790"
     CDS             <1..830
                     /gene="purM"
                     /locus_tag="VF995_00790"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX9342136.1"
                     /translation="VGTKLLVARALDRHDTVGLDLVGMVVDDLVASGARPLFLLDYLA
                     VGRLVPERVAAIVKGVAEGCRQAGCALLGGETAEHPDAMEPDAYDLAGFAVGMVERER
                     LLGPERVRPGDVLLALASSGLHSNGFALARRVLDRAGVGYDRVFPELGSPIGEALLEP
                     TRIYAPDLLALLDGDVEVHALCHITGGGLPGNLPRSLPAGLVPQVDWASWTPPAIFGL
                     LAGLGPVEEVELRRATNLGVGMVAILPAAAADRARAFLAPRGLHTWIMGEVVPSTTG"
     gene            complement(820..1878)
                     /locus_tag="VF995_00795"
     CDS             complement(820..1878)
                     /locus_tag="VF995_00795"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009373861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX9342137.1"
                     /translation="MGAFELMGGEYEQVVYNHDPASGLRAIIAIHSTALGPALGGTRF
                     YPFANEDDALRDVLRLARGMSYKAAAAGLDLGGGKAVIIGDPKRIKTEALLRAHGRFV
                     ESLGGRYITAEDVGTFTADMDVVARETRFVTGRSESHGGGGDPSINTAYGVFCGLRAV
                     AEALWNQHDLAGRHLVVQGVGKVGHHLCRLLSQAGARLTVSDVDLDHVGRAVNEFGAD
                     TADPGKVHAVECDILVPCALGGVINDDTLPELKCQAVAGSANNVLALPEHGYALRELG
                     ILYAPDYVINAGGLINVADELQGYSAERARTRVEGIFGALREVFHRAKEEGIPTNQAA
                     DKLAEQRMREISRIRLIR"
     gene            1998..2840
                     /locus_tag="VF995_00800"
     CDS             1998..2840
                     /locus_tag="VF995_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342138.1"
                     /translation="MNGHAAAAYLRVFQPLAAYSPGERSGWAAYVEAGQTLPASVLVG
                     QEERRGLARALGLAVGADPEHALVIRDSEGGTVFVCPLRSELRRLQSMVAFSTSLDSL
                     PHEVREAFLSPDDLARAEQELERLHGEAPEARNQMLQAVWHVPLHWFALFSAEERQLR
                     PAQGGQPASLTYQTTMSSARMRVSRALGILRDLMQDADVVAAVEELREWLYAFDPDSL
                     VQLDYAGLASVIAYPDLVADLSAGEIREALEALEEGDLERSSELYRLLTERWATVRGR
                     EASN"
     gene            3024..>3179
                     /locus_tag="VF995_00805"
     CDS             3024..>3179
                     /locus_tag="VF995_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HEX9342139.1"
                     /translation="MSASRNIQNAPTPEALLTPAEVAALFRVDPKTVTRWAKAGKLSS
                     IRTLGGHR"
BASE COUNT          430 a         1098 c         1151 g          500 t
ORIGIN      
        1 gggtcggcac caagctgctg gtcgcgcggg cactcgaccg gcacgacacc gtcggcctcg
       61 acctggtcgg catggtcgtc gacgacctgg tggcgagcgg ggcgcggccg ctgttcctgc
      121 tcgactacct ggccgtcggc cgcctggtcc ccgaacgggt cgccgccatc gtcaagggcg
      181 tggccgaggg ctgccgccag gccggctgcg ccctgttggg cggcgagacc gccgagcacc
      241 ccgacgccat ggagccggac gcctacgacc tggccgggtt cgcggtcggc atggtggagc
      301 gcgagcgcct cctcggcccc gagcgggtgc gccccggcga cgtgctgctc gccctcgcct
      361 cctccgggct gcactccaac ggcttcgccc tggcccgccg ggtcctcgac cgcgccgggg
      421 tcggctacga ccgggtcttc cccgagctgg gcagcccgat tggcgaggcg ctgctggagc
      481 cgacccgcat ctacgccccg gacctgctcg ccctgctcga cggcgacgtc gaggtccacg
      541 ccctgtgcca catcaccggc ggcggcctgc ccggcaacct gccccgcagc ctccccgccg
      601 gcctggtccc ccaggtggac tgggcctcct ggacgccccc cgcgatcttc gggctgctgg
      661 ccggtctcgg cccggtggag gaggtcgagc tgcgccgcgc caccaacctg ggcgtcggca
      721 tggtcgccat cctccccgcc gccgcggccg accgcgcccg tgcgttcctc gccccccgcg
      781 gcctccacac ctggatcatg ggcgaggtcg tcccctcgac taccggatga ggcggatccg
      841 ggagatctcg cgcatgcgtt gttcggcgag cttgtcggcg gcctggttgg tggggatgcc
      901 ctcctccttg gcgcggtgga agacctcgcg gagggcgccg aagatgccct cgacgcgggt
      961 acgtgcgcgc tcggcggagt agccctgcag ttcgtcggcg acgttgatga ggccgccggc
     1021 gttgatcacg tagtcgggcg cgtagaggat gccgagctcg cgcagggcgt agccgtgctc
     1081 gggcagggcc agcacgttgt tggccgagcc ggcgacggcc tggcacttca gctccggcag
     1141 ggtgtcgtcg ttgatcacgc cgccgagggc acagggcacg aggatgtcgc attcgaccgc
     1201 gtggaccttg ccggggtcgg cggtgtcggc gccgaactcg ttgaccgccc ggccgacgtg
     1261 gtccaggtcg acgtcggaga cggtgagccg cgcgccagct tgactcagga ggcggcagag
     1321 gtggtggccg accttgccga cgccctggac gaccaggtgc ctgccggcca ggtcgtgctg
     1381 gttccacagc gcctcggcga ccgcccgcag gccgcagaag accccgtagg cggtgttgat
     1441 cgaggggtcg ccgccgccgc cgtggctttc ggagcggccg gtgacgaagc gggtctcgcg
     1501 ggccaccacg tccatgtcgg cggtgaaggt gccgacgtcc tcggcggtga tgtagcggcc
     1561 gccgaggctc tcgacgaagc ggccgtgggc ccgcagcagc gcctcggtct tgatccgttt
     1621 ggggtcgccg atgatgacag ccttgccgcc gccgaggtcg aggccggcgg ctgccgcctt
     1681 gtagctcatg ccgcgggcca gccgcaggac gtcgcgcagc gcgtcgtcct cgttggcgaa
     1741 ggggtagaag cgggtgccgc cgagcgccgg tccgagcgcc gtcgagtgga tggcgatgat
     1801 ggcccgcagg ccgctggccg gatcgtggtt gtagacgacc tgctcgtact cgccacccat
     1861 gagctcgaac gctcccatgg atgacctccg ttggtcaggc cccaggcggg ttcgccaccg
     1921 gagcgactcc cggccaaagg gatacccggc cgggatctca acgtatcaca gggcccagcg
     1981 acttatactc tcccctcgtg aacgggcatg ccgcagcggc gtacctgcgg gtcttccagc
     2041 cgttggccgc ctactcgccg ggggagcgct ccgggtgggc cgcctacgtc gaagccggcc
     2101 agacgctccc ggcaagcgtc ctggtgggtc aggaggagcg tcgcggcctc gcgcgggcgc
     2161 tcggcctcgc cgtcggcgcc gatcccgagc acgcgctggt catccgtgac agcgagggcg
     2221 ggaccgtctt cgtctgcccg ctgcgcagcg agctgcgccg gctgcagtcg atggtggcct
     2281 tcagcacctc gctggactca ctgccccacg aggtgcggga ggcgttcctc tcgcccgacg
     2341 acctggcgcg ggccgagcag gagctcgaac gactgcacgg cgaggcgccg gaggcgcgca
     2401 accagatgct gcaggccgtc tggcacgtgc cgttgcactg gttcgcgctg ttctccgccg
     2461 aggagcggca gctgcgcccg gcgcagggcg gccagccggc aagcctcacg taccagacga
     2521 cgatgtcgtc ggcgcgcatg cgggtgtctc gagccctggg gatcctgcgc gatctgatgc
     2581 aggacgcgga cgtggtcgcg gctgtggaag agttgcggga gtggttgtac gcttttgacc
     2641 cggacagttt ggtacaactg gactatgctg gtctagcgag cgtgatcgcc tacccggatc
     2701 tggtcgccga tctgtcggcc ggggagatcc gggaggcgct cgaagcgctc gaggaaggtg
     2761 acctggagcg ctccagtgag ctgtatcgcc tgctcacaga gcgttgggca acggtgcgag
     2821 gacgggaagc aagcaattga tctctcctga tctctccgcc cgttttgcta aagaccggtc
     2881 ggtcacatgc cgatagtcct agtaggcggg gggagaaata gccctaagtt gctatggtct
     2941 gtcccattcg acgcctctag gataatcagg atctccgcga gaatcaggac tcgtggggag
     3001 cgtaccaagg tgaggtggct accatgtccg ccagtcgcaa catccagaac gcgcccacac
     3061 ccgaggcgct gctgaccccg gccgaggtcg ccgcgctgtt tcgcgtcgac cccaagacgg
     3121 tcactcgctg ggccaaggca ggaaagctct cgagcatcag gacgctcggc gggcaccgc
//
LOCUS       DASWDC010000027         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_336981, whole genome shotgun sequence.
ACCESSION   DASWDC010000027 DASWDC010000000
VERSION     DASWDC010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336981"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..627
                     /locus_tag="VF995_00810"
     CDS             31..627
                     /locus_tag="VF995_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342140.1"
                     /translation="MRMSGVWGRWRRKPPVVAPPRRASDGSGGGNGDGTGTDQGPSGA
                     GAAAAPRGHVAILLDPGLESVASSLQEELGPGFAVVVEPPEPVGSSQSVIAVLDATPD
                     PAASTAVARQRRRHPQAGMLVVASRSAAAATWQTTADLLDAGADDVLIELNTRELAAR
                     LKALARRRLATPATPATPAPRMNDPVGHPADDPARSLT"
     gene            813..1493
                     /locus_tag="VF995_00815"
     CDS             813..1493
                     /locus_tag="VF995_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342141.1"
                     /translation="MVRTWALARTYRRGSIPVPVLRGINLELREGDWVAILGPSGSGK
                     STLLNLLGLLDQPDAGVYELAGEDVSDVSDTRRAELRNREIGFVFQRFNLLPRTSALE
                     NVAAPLLYRRIRRAERLERAAQVLEEVGLGDRMDYDTSELSGGQIQRVAIARALVGEP
                     SLLLADEPTGNLDSASGADILDLFGRLHARGRTILLITHDPVVAGRAERRLLLDDGRL
                     HLMDHSQN"
     gene            1576..2328
                     /locus_tag="VF995_00820"
     CDS             1576..2328
                     /locus_tag="VF995_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342142.1"
                     /translation="MESNRPPNPPPDPEAGPPAERAGERSDAVHGRPAEPPWAQLPPG
                     IEAGPPPAADSDGDGDSDHGPVPVYDRVETRTNVRAVLLAAALALAVGAAGGFFVGRQ
                     SAKKTPTTLAQAIQMAASGQLPRGNVGAGRFGAFGGGGGGGNGQNGTGNAQGGQGAGG
                     GRGVGGFGGFQGSITAVDGNTITVQTPAGPVKVVVAGNTSILKTSSGTRSDLAVGKTV
                     QVRLNLSASNGGSGGNTSGTVTASSIVAEPTQ"
     gene            2325..>3197
                     /locus_tag="VF995_00825"
     CDS             2325..>3197
                     /locus_tag="VF995_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX9342143.1"
                     /translation="MSKAKSKARPSGSARRRRRRNGWIAAGVVLVALAGAGAAYLVTA
                     GSSKAVVAQPPATSPARRGNLTQTVDASFTLVRDPAITLTSPQGGIITKLDLSNGAKP
                     ASFKPLLAVNGTNVYAIVSSTPIYRSLASGDSGDDVKALQTALKADGYDPGTIDGTYG
                     TGTFDAVEAWQTDQALTVDGKFNLSDFVWVRPGSTALSVSASVGDRITAQTALATIAV
                     PGALVAQADVSQLDVGRLKAAQSAQLTFDALSGATAPAKVVAIPVDSESSQQSAGSNS
                     VVQYAVTLRPLRLPA"
BASE COUNT          476 a         1191 c         1130 g          400 t
ORIGIN      
        1 tccgtgggaa cgggggagag gagctggcag atgcggatga gcggcgtgtg gggacgctgg
       61 aggcgcaagc ccccggtggt cgcgccgcca aggcgcgcca gcgacggcag cggcggcggc
      121 aacggcgacg gaactgggac cgatcaggga ccgtccgggg ccggggctgc cgccgcgccc
      181 agggggcatg tcgcgatcct gctcgacccg gggctcgaat cggttgccag ctccctgcag
      241 gaggagctcg ggcccgggtt cgcggtggtc gtggaaccgc cggagccggt ggggtcgtcg
      301 cagagtgtta tcgcggtgct cgacgcgacc cccgatcccg ccgcgtcgac ggccgtcgcc
      361 cgccagcgcc gccggcaccc gcaggcgggc atgctggtgg tggcgagccg gagcgccgcc
      421 gccgccacct ggcagacgac cgccgacctg ctggacgccg gtgccgacga cgtgctgatc
      481 gagctgaaca cccgcgagct ggccgcccgg ctgaaggcgc tcgcccggcg gcggctggca
      541 acgcccgcca cgcccgccac gcccgctccc cggatgaacg acccggtggg ccaccccgcc
      601 gacgaccccg cacggtcgct cacctagcgg ctgccgctca gggtgtctgt ggcccgcccg
      661 ctggccagtt cgtgaccggg cgaaactcac ggcttgccct cagccggccc tcagctggcc
      721 tcaagcttcg gtcctgagga tcaccgctgc aggcggggtg tgcctgcctg ccttccttcc
      781 tttcgccgtc gtcgaggagg cattgggtgc acatcgtgcg gacctgggcg ctggcacgga
      841 cctaccggcg cgggtcaatc ccagtgccgg tcctgcgtgg catcaacctg gagctccggg
      901 aaggcgactg ggtcgccatc ctgggtccct ccggctcggg gaagtccacc ctgctcaacc
      961 tgctggggct gctcgaccag ccagacgcgg gcgtctacga gctggccggc gaggacgtct
     1021 ccgacgtcag cgacacgcgc cgggccgagc tgcgcaaccg cgagatcggg ttcgtgttcc
     1081 agcgcttcaa cctgctgccg cggaccagcg cgctcgagaa cgtggcggcg ccgctgctct
     1141 accggcggat ccgccgggcc gaacggctgg agcgcgccgc ccaggtgctc gaagaggtgg
     1201 gcctcggcga ccgcatggac tacgacacca gcgagctgtc tggtgggcag atccagcggg
     1261 tcgcgatcgc ccgcgcgctg gtcggcgagc cgtcgctgct gctggccgac gagccgaccg
     1321 gcaacctcga cagcgcctcg ggggccgaca tcctggacct gttcggccgc ctgcacgcgc
     1381 gcggtcggac catcctgctg atcacccacg acccagtcgt cgccggtcgt gcggagcggc
     1441 ggctcctgct cgacgacggc cgcctccacc tcatggacca tagccaaaac tgactatcca
     1501 aatgtagggg acggtaagct aggttaagcg aagctaaacg agataaacga atggtaagag
     1561 ctggaggagt cgctcatgga gtccaaccgc ccgcccaacc cgccacctga cccggaggcc
     1621 ggcccgccgg ccgagcgtgc aggcgagcga agcgatgccg tccacgggag gccggcggag
     1681 ccgccctggg cgcagctccc gcccgggatc gaggccggac cgcctcccgc cgccgacagt
     1741 gacggcgacg gcgacagcga ccatggcccc gtccctgtct acgaccgcgt cgagacgcgg
     1801 accaacgtcc gggcggtgct gctcgccgcg gcgctggcct tggcggtggg cgccgccggc
     1861 gggttcttcg tcgggcgcca gagcgccaag aagaccccga ccactctggc gcaggcgatc
     1921 cagatggccg ccagcgggca gctgccgcgc ggcaacgtcg gcgctggccg cttcggcgcc
     1981 ttcggtggtg gcggtggcgg tgggaacggc cagaacggca ccggcaacgc ccagggcggc
     2041 cagggcgccg gcggaggccg cggcgtcggc ggcttcggcg ggttccaggg cagcatcacc
     2101 gccgtcgacg gcaacaccat caccgtccag accccggccg gcccggtcaa ggtcgtggtc
     2161 gccggcaaca cctcgatcct caagacctcc tcgggcaccc gctccgacct tgccgtcggc
     2221 aagaccgtcc aggtccgcct caacctgagc gccagcaacg gcggcagcgg cgggaacacc
     2281 tccggaaccg tgaccgcctc cagcatcgtg gcggagccga cccaatgagc aaggccaaga
     2341 gcaaagcgcg ccccagcggc tccgccaggc ggcgccggcg ccgcaacggc tggatcgccg
     2401 ctggtgtcgt actggtggcg ctggccggcg ctggcgccgc ctacctggtg accgctgggt
     2461 cgagcaaggc ggtggtggca cagccgccgg cgacctcgcc ggcgcggcgg ggcaacctca
     2521 cccagacggt cgacgcctcg ttcacgctgg tccgcgaccc ggcgatcacc ctgacctcgc
     2581 cacagggggg gatcatcacc aagctggacc tcagcaacgg cgccaagcca gcgtcattca
     2641 agccgctgct ggcggtcaac ggcaccaacg tctacgcgat cgtcagctcc acgccgatct
     2701 accgcagcct cgccagtggc gacagcggcg acgacgtcaa ggccctgcag accgcgctga
     2761 aggccgacgg ctacgaccct ggcaccatcg acgggaccta tggcaccggc accttcgacg
     2821 cggtcgaggc ctggcagacc gaccaggcgc tgaccgtcga cggcaagttc aacctgtcgg
     2881 acttcgtctg ggtccggccc ggctcgaccg cgctgagcgt ctccgccagc gtcggcgacc
     2941 gcatcaccgc ccagacggcg ctggcgacca tcgccgtccc cggcgcgctg gtggcgcagg
     3001 ccgacgtcag ccagcttgac gtcggccgtc tcaaggcagc ccagagcgcc cagctcacct
     3061 tcgacgccct ctccggcgcg accgcgcccg ccaaggtggt cgcgatcccg gtggactcgg
     3121 aaagctcgca gcagagcgcc ggctcgaact cggtggtcca gtacgcggtg accctgcggc
     3181 cgctgcgcct gcccgcc
//
LOCUS       DASWDC010000028         3982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1341397, whole genome shotgun sequence.
ACCESSION   DASWDC010000028 DASWDC010000000
VERSION     DASWDC010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341397"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VF995_00830"
     CDS             <1..181
                     /locus_tag="VF995_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342144.1"
                     /translation="SDDNPDTVAARLVTYRRDAGPLLAHYSGRVRRVSASGDLDEVFE
                     RAERALASLPRAARG"
     gene            complement(197..1675)
                     /locus_tag="VF995_00835"
     CDS             complement(197..1675)
                     /locus_tag="VF995_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342145.1"
                     /translation="MSVASGARTLSPTRIDLGAPAAERDIERGLAAYFIESEAFTRVR
                     HGEKLVILGSRGAGKSAIFQMLAQREREAGSHVIELAPEDYSYELLHQTMLPENSGSW
                     AKLGAYAVAWKYLIYIQVMQELSRKGQRLKGPAVAMYRYVRDNHVGGATSKLSALISY
                     LKRVEGLKVGPYEASLRTRELEKLYKLAELDPLFPALKQVLERERVVVLVDELDRGWD
                     ASEDAQAFVAGLFQACMSINGLSDNLKVYMSLRQELYDNIPALYDDAQKFRDVIEVLS
                     WDPSSLLQLIAARIRHSVPEMRKADDRHCWNLIFSPSVAGSPGSSFGFVMDRTLHRPR
                     EVIQLCAHALEETRNRKLSLPVTPDTLAKVELAYSEERTKDIAAEFRFQYPGLLHVFE
                     TFRGGPSHLDREELSWHCLELTDGAALRSGAREWVSQESIDSLIDILWQVGFLQADSP
                     GANTAYLGPYQVGHLNLATTQRFRVHPMFWAYLALNLKDSDS"
     gene            1857..3596
                     /locus_tag="VF995_00840"
     CDS             1857..3596
                     /locus_tag="VF995_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX9342146.1"
                     /translation="MRPREDLREQHRHVPPVAHARLRPRPRPRPWPQPPTPDPAPVPA
                     ATAAAAAAARPRWTRRQVLAGGGAALGLGLVGVLGWKGAWAEARLGEARAALGDLGLP
                     LPGLHGPGAASASAGIADGAGGGGARTVRATLSAQPVTIDLGGPVVRTWAYGDALPGP
                     LLRGRVGDTLEVAVRNHLPQATTVHWHGMAIPNPMDGVPNLTQQPIAPGAAMTYRFVL
                     PHPGTYWYHPHVGVQLDRGLYGPLIVDDPADPGRYDAEFTVVLDDWLDGTGRTPDQVL
                     QQLAGGMEMDMSGMSMGEAAGMASMRSAALGGDAGDVDYPHYLVNGRVPSAPVTLRAR
                     PGQRVRIRLLNAGADTAFRVALGGHRLTVTHSDGYPVRPVTVDSLLIAMGERYDLEVI
                     LGDGAFPLVAEAEGKGARGLAVVRTAGGAIPGAAAKPAELRGRLLRLSDLDAAPEAAL
                     PRQRLDRVHDMVLQGDMAGYRWTINGHSYDHLRPSDAPALPLRDGERLRLRFVNLSPM
                     FHPMHVHGHTFQVRTAGSASGAGPRKDTVLVRPLETVVCDLAADNPGTWMAHCHNLYH
                     AESGMMALLAYQN"
     gene            complement(3659..>3982)
                     /locus_tag="VF995_00845"
     CDS             complement(3659..>3982)
                     /locus_tag="VF995_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9342147.1"
                     /translation="SRLVVAPRFYPSSKRCSGCGQVTDTLPLGVRVFSCPRCGLMVDR
                     DLNAARNLAQLAEDRNGWVAGSSPETENACGAGSAGQTGDGLVELPAVKQERARIRGH
                     AITDA"
BASE COUNT          557 a         1480 c         1380 g          565 t
ORIGIN      
        1 cagcgacgac aaccccgaca ctgtcgccgc ccgcctggtc acctaccgcc gcgacgccgg
       61 cccgctcctg gcccactact ccggccgcgt ccgccgcgtc agcgcctccg gcgacctcga
      121 cgaggtcttc gaacgcgccg aacgcgcgct cgcgtccctt ccccgcgccg cccgcggctg
      181 aactgagcgg cctagcctag ctgtcgctgt ccttcaggtt gagggcgagg tacgcccaga
      241 acatcgggtg gacgcggaac cgctgggtgg tggcgaggtt gaggtggccg acctggtagg
      301 gcccgaggta ggcggtgttg gcgccggggg agtcggcctg caggaagccg acctgccaga
      361 ggatgtcgat cagggagtcg atgctttcct ggctgaccca ttcgcgggcg ccgctgcgca
      421 gggcggcgcc gtcggtcagc tccaggcagt gccacgacag ctcttcgcgg tcgaggtggg
      481 aggggccgcc gcggaacgtc tcgaagacgt ggagcaggcc ggggtactgg aagcggaact
      541 cggcggcgat gtccttggtg cgttcctcgg agtaggccag ttcgaccttg gcgagggtgt
      601 cgggggtgac cgggaggctc agcttgcggt tgcgggtctc ctccagggcg tgggcgcaga
      661 gctggatgac ctcgcgtggg cggtgcaggg tgcggtccat gacaaacccg aaggagctgc
      721 ccggtgaccc cgccaccgag ggggagaaga tcaggttcca gcagtgccgg tcgtcggcct
      781 tgcgcatctc cgggaccgaa tggcggatcc gggccgcgat cagctgcagc aggctcgacg
      841 ggtcccagga gagcacctcg atcacgtcgc ggaacttctg cgcgtcgtcg tacagcgccg
      901 ggatgttgtc gtacagctcc tggcgcagcg acatgtagac cttcaggttg tcgctgaggc
      961 cgttgatcga catgcacgcc tggaacaggc cggcgacgaa cgcctgcgcg tcctcggagg
     1021 cgtcccagcc gcggtccagc tcgtcgacca gcaccaccac ccgctcgcgt tccaggacct
     1081 gcttgagggc ggggaacagc gggtccagct cggcgagctt gtagagcttc tccagctccc
     1141 gggtgcgcag cgacgcctcg taggggccga ccttcaggcc ctcgacccgt ttcaggtagg
     1201 agatcagcgc cgagagcttg ctggtggcgc cgccgacgtg attgtcgcgg acgtagcggt
     1261 acatggccac cgccgggccc ttgagccgct ggcccttcct ggacagctcc tgcatcacct
     1321 ggatgtagat caggtacttc caggcgaccg cgtaggcgcc gagcttggcc caggagccgg
     1381 agttctccgg gagcatcgtc tggtgcagca gctcgtagga gtagtcctcg ggcgccagct
     1441 cgatgacgtg gctgccggcc tcgcgctccc gctgggcgag catctggaag atggcgctct
     1501 tgccggcgcc ccggctgccg aggatgacca gcttctcacc gtgccggacg cgggtgaacg
     1561 cctcggattc gatgaagtag gcggccagtc cccgctcgat gtcccgctcg gccgctggag
     1621 cacccagatc gatccgggtc gggctgagcg tccgggcgcc agaggcgaca ctcatgtact
     1681 tccccctcag tccggttgga tggacacgga cgccttgacc ctaccggacc aaccccatcg
     1741 gcgcatagag gaataaagcg gggcgagggc gccgggctgc tgtggttacc ggctcgttag
     1801 cccgctccgc gaagttcatg gctgcctcgt cacccgaccg aaccggagcc gccttcatgc
     1861 gccctcgcga ggacctccgc gagcagcacc gtcacgtccc acccgtcgcc cacgcccggc
     1921 tccgcccccg cccccgcccc cgcccctggc cgcagccgcc aaccccggac ccagccccgg
     1981 tgcccgctgc cacggctgcg gctgcggcag cggcgaggcc gcggtggacg cgccggcagg
     2041 tgctcgccgg cggcggcgcg gcgctcggcc tcggcctggt cggcgtgctg ggctggaagg
     2101 gggcgtgggc ggaggcgcgg ctcggcgagg cccgcgccgc cctcggcgac ctcggcctgc
     2161 cgcttccggg gctccacggc cccggtgcgg cctcagcgtc ggcggggatc gccgacggcg
     2221 ccggcggcgg cggcgcgcgg acggtccgcg ccacgctctc cgcccagccg gtcaccatcg
     2281 acctcggcgg gccggtggtg cggacgtggg cctacggcga cgccctgccc ggcccgctgt
     2341 tgcgcgggcg ggtcggcgac acccttgagg tggcggtgcg caaccacctc ccgcaggcga
     2401 ccacggtgca ctggcacggc atggccatcc cgaacccgat ggacggcgtg ccgaacctca
     2461 cccagcagcc gatcgcgcca ggcgcggcca tgacctaccg cttcgtcctg ccccaccccg
     2521 gcacctactg gtatcacccg cacgtcggcg tgcagctcga ccggggcctg tacgggccgc
     2581 tgatcgttga cgaccccgcc gacccgggcc gctacgacgc cgagttcacc gtggtgctcg
     2641 acgactggct cgacggcacc ggccgcaccc ccgaccaggt gctccagcag ctcgccggcg
     2701 gcatggagat ggacatgagc ggcatgtcga tgggcgaggc ggcgggcatg gcgagcatgc
     2761 gctcggcggc gctcggcggc gacgccgggg acgtcgacta cccgcactac ctggtcaacg
     2821 gccgggtgcc gtccgcgccg gtgacgctgc gggcgcggcc cggccagcgg gtccgcatcc
     2881 ggctgctcaa cgccggggcg gacaccgcct tccgggtcgc cctgggcggc caccgcctca
     2941 ccgtcaccca cagcgacggc tacccggtcc gcccggtcac ggtcgactcg ctgctgatcg
     3001 ccatgggaga gcgctacgac ctggaggtga tcctcggcga cggcgccttc ccgctggtcg
     3061 ccgaagccga gggcaagggc gcccgggggc tcgcggtggt ccgcaccgcc ggcggcgcca
     3121 tccccggcgc cgccgccaaa ccggcggagc tgcgcggccg gctgctgcgc ctcagcgacc
     3181 tggacgccgc ccccgaggcc gccctcccgc gccagcgcct cgaccgggtc cacgacatgg
     3241 tcctgcaggg cgacatggcg ggctaccgct ggaccatcaa cggccacagc tacgaccacc
     3301 tgcgcccaag cgacgccccg gcgctgccgc tccgcgacgg cgagcggctg cggctgcgct
     3361 tcgtcaacct gagcccgatg ttccacccga tgcacgtcca cggccacacc ttccaggtcc
     3421 gcaccgccgg cagcgccagc ggcgccgggc cgcgcaagga caccgtgctg gtgcggcccc
     3481 tggagacggt cgtctgcgac ctcgccgccg acaaccccgg cacctggatg gcgcactgcc
     3541 acaacctcta ccacgccgag agcggcatga tggccctgct cgcctaccag aactagcgcg
     3601 aacgagggcg ctgcgcctct ctctctctct acactgcttg gacgccgttc atcggaatct
     3661 acgcatccgt gatcgcatgg ccacgaatgc gagcccgttc ctgcttcacc gcaggcagtt
     3721 ccaccaaacc gtcaccggtc tggccagcgc tccccgctcc acaggcgttt tcggtctccg
     3781 gggaactccc ggcgacccag ccgttcctat cttccgcaag ctgtgccagg ttcctggccg
     3841 cattcaggtc gcggtctacc atcagcccgc acctcgggca gctaaacacc cgcaccccaa
     3901 gcggcagggt gtccgtgacc tgcccgcagc ccgagcagcg cttggaggag gggtaaaacc
     3961 gtggcgcgac taccagccgg ga
//
LOCUS       DASWDC010000029         5698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725 k141_6675,
            whole genome shotgun sequence.
ACCESSION   DASWDC010000029 DASWDC010000000
VERSION     DASWDC010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6675"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VF995_00850"
     CDS             <1..728
                     /locus_tag="VF995_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9342148.1"
                     /translation="VAVDDAFGDAVSRGAAGELRERHYTQAGTVRYDPRRYNASQVVH
                     QIAATRPDVLFAVAYIKDGIALRRETVRQHLHLVANIGTSSSYCMLAFGAALGRDAVG
                     LFASDKPDADSLNQQALNPQARELLSTAQREYRAAYHQEMSAAALAGFAGAWALFHWA
                     LPSASSDSPAEVARAARALRLPTGTLPNGSGLRFGQVGTPDATVNLRATSVIWEWTAV
                     DRRQVIWPPPFATGQFRPLPMAA"
     gene            725..1522
                     /locus_tag="VF995_00855"
     CDS             725..1522
                     /locus_tag="VF995_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342149.1"
                     /translation="MSGRTGRRMNLRGEGGRRILLAGTGAVLVLAYLAGASLSGALSP
                     LARRPLLDGLAPPPPYRWVSPPPEFASGNKPPSSGHATIRLGAGGSEVSASGTDDGQL
                     SLVFDQGAVAASAGQQSATITIQPFDPAKLAPVPSGLIAAGNAYRIQARYQPSGKAAS
                     IVKGQVALIYPLLSVPVASPFDYLVLTSPDGHAWTRQRSTTQPGSHQVSAPVSGGGYV
                     LVAVPPGTAAQSQGHSRRVLVLLIVAVVYAIIMVIVVGRWLVRRSAR"
     gene            1519..2658
                     /locus_tag="VF995_00860"
     CDS             1519..2658
                     /locus_tag="VF995_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342150.1"
                     /translation="MRRSQWRRRLVLAPLLAVVLAMAGATPASAHVIGTSAKPGNYRT
                     RVLAINPAVPGLTVRVVEAGGKLELINHSGQAVLVLGYDNEPYLRVGPSGVDENERSP
                     STWSNRSVLPPKTRPPSGLDPSAPPRWRRVSGRPVAIWHDHRSHWAGPGEPPAVRADR
                     GRTQVVVPEWRVPLRVGSQTVVVSGEIAWVPGPAPWPWIGAAVLLCAAVLLAARRGGA
                     WRLRALIAATVLAVAADVVHATGSWLGSTASTVTKLYSNATPLAGWVVAGLAIWRLLR
                     GRTANGSGSPDALETGNTYLLIAGIFLAVAGALPDVGVLDSSQIPEPFPPNLTRAVVT
                     ITLGLGSALVLAVLLAPPTTPRRPKPAPDPSGNQAAPSPSPPQPS"
     gene            complement(2678..3862)
                     /locus_tag="VF995_00865"
     CDS             complement(2678..3862)
                     /locus_tag="VF995_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9342151.1"
                     /translation="MRLHEQLRERRGMALTIVVGAAAVAVHLWLIRAAILGARRWLIS
                     PVVIGVAVILVLALHGAGLRQLVRRLRPHPHGHQHSHDTGHRIDSALTASAEGMRALR
                     ISLVVLGATAVAQFVVVLASGSVALLSDTIHNFADALTAIPIGIAFLAGRRLPNRRYT
                     YGYGRGEDVAGLFVLLAMALSALIAAYEAVDHLVNPRDLRNLGWVAAAGIVGFIGNEW
                     VAAYRIRVGRRIGSAALLADGLHARTDGLTSLAVVAGAVGVAAGWRAADPVAGLAIGI
                     AIIGVLRQAVREIYGRMMDRVDDHTVLHAEEVIRQVPGVVSLDRLRMRWVGHDLLAEV
                     EIAADASLSLPQAHDLAEAVRHQLLHQVPRLADATVHVGHQPTLGGDAHRETSHHFSQ
                     RH"
     gene            4002..4652
                     /locus_tag="VF995_00870"
     CDS             4002..4652
                     /locus_tag="VF995_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9342152.1"
                     /translation="MPRPLAAPVLAATDGDLEAVREHILEATHRVIDRKGLAAASTRA
                     IAEEAGVAGGTLYNYFDNHTQLLAKAIVWYAAKLSDPVASLPSRAGKYTVAHNLRYFV
                     RQAATVLDRLVPAFAAAFSNSQLLEAVRREMAGIDPLSDPARVVERYLLAERDLGRVA
                     PDAECRTAASIIVSLCHDDAYQRYLYRETGPPRPRRGEIDLIARSLTKPLTKPLTE"
     gene            4671..5339
                     /locus_tag="VF995_00875"
     CDS             4671..5339
                     /locus_tag="VF995_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9342153.1"
                     /translation="MYLIIGIVAAAAVIVAAALLALAVRALKAAHTRPPAGAAAAAPH
                     TQEHGHGGSGLQRTVLSIVGRTIPRLLRLGVRMGPMMLLTVRGRSTGLPRTNPVDLFE
                     RDGRHWLVATHEANASWVRNLRAAGEGTLARGRRRYGFTAVELSQRAAGTVLKEVLGP
                     RLTRPAAGFVLRQTLGVPPGGSLDEFTSAAASHPVFELTIRRDGALAPTSGIASQPQR
                     KETA"
     gene            5336..5527
                     /locus_tag="VF995_00880"
     CDS             5336..5527
                     /locus_tag="VF995_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342154.1"
                     /translation="MSTPATNSKKAPMVAIGAGILIVLAHLTLAVTGVMSAAQWTSGV
                     VIGLLIAGVGNHFRIFGRR"
BASE COUNT          795 a         2179 c         1982 g          742 t
ORIGIN      
        1 tggtggcggt ggacgacgcc ttcggcgacg cggtcagccg cggcgccgcc ggcgaactgc
       61 gcgagcgcca ctacacccag gccggcacgg tgcgctacga cccgcgccgc tacaacgcca
      121 gccaggtcgt ccaccagatc gccgcgaccc ggccggacgt gctgttcgcg gtcgcctaca
      181 tcaaggacgg catcgccctg cggcgcgaga ccgtgcgcca gcacctgcac ctggtcgcca
      241 acatcgggac ctcgtcgagc tactgcatgc tggccttcgg cgcggcgctc ggccgtgacg
      301 ccgtcggcct gttcgcctcc gacaagcccg acgccgactc cctcaaccag caggccctca
      361 acccgcaggc acgcgagctg ctgagcaccg cccagcggga ataccgcgcc gcctaccacc
      421 aggagatgag cgcggcggcg ctggcgggct tcgcgggcgc ctgggcgctg ttccactggg
      481 ctctcccctc cgccagctcc gacagcccgg ccgaggtggc cagggccgca cgggcgctgc
      541 ggctgccgac cggcaccctg cccaacggca gcggcctgcg gttcggccag gtcggcacgc
      601 ccgacgccac cgtgaacctc cgcgccacca gcgtcatctg ggagtggacc gcggtggacc
      661 gccgccaggt gatctggccg ccgccgttcg cgaccggcca gttccggccg ctccccatgg
      721 ccgcatgagc ggccggacgg gcagacggat gaacctccgg ggggagggcg ggcggcggat
      781 cctgctcgcc gggaccggcg cggtgctggt gctcgcctac ctcgccggcg cctcgctcag
      841 cggcgcgctg agcccgctgg cgcgccggcc gctgctggac gggctggcgc cgccgccgcc
      901 ctaccgctgg gtgagcccgc cgccggagtt cgccagcggc aacaagccgc cgagcagcgg
      961 ccacgcgacc atccgcctcg gcgccggcgg ctccgaggtg agcgccagcg gcaccgacga
     1021 cggccagctc agcctggtgt tcgaccaggg ggcggtcgcg gccagcgccg gccagcagtc
     1081 ggcgacgatc accatccagc cgttcgaccc ggcgaagctg gcaccggtcc cgtccgggct
     1141 gatcgccgcc ggcaacgcct accgcatcca ggcgcgctac cagccctccg ggaaggcggc
     1201 ctcgatcgtc aagggtcagg tggcgctgat ctacccgctg ctctcggtcc cggtggccag
     1261 ccccttcgac tacctcgtgc tcacctcccc cgacggccac gcctggaccc gccagcggag
     1321 caccacccag cccggcagcc accaggtgag cgccccggtc tccggcggcg gctacgtcct
     1381 ggtcgctgtc cccccgggaa ccgccgccca gagccagggc cacagccgcc gtgtcctggt
     1441 cctgctgatc gtcgcggtcg tgtacgcgat catcatggtg atcgtggtcg gccggtggct
     1501 ggtgaggagg tccgcgcgtt gagacggagc cagtggcggc gccggctggt gctggcgccg
     1561 ctgctggccg tggttctggc aatggctggc gccacgcccg cgagtgcgca tgtcatcggg
     1621 accagcgcca agccgggcaa ctaccggacc cgggtgctcg ccatcaaccc cgcggttccc
     1681 ggcctcaccg tccgggtggt cgaggccggc ggcaagctgg agctgatcaa ccacagcggc
     1741 caggccgtgc tggtgctcgg ctacgacaac gagccctacc tgcgcgtcgg gccgtccggc
     1801 gtggacgaga acgagcgctc cccgagcacc tggtccaacc gcagcgtgct gccgcccaag
     1861 acccgcccgc cttccggcct ggacccgtcg gcgccgccgc ggtggcgccg cgtctccggc
     1921 aggcccgtgg ccatctggca cgaccaccgc agccactggg ccggaccggg cgagccgccc
     1981 gccgtccgag cggatcgagg gcggacgcag gtggtcgtgc ccgagtggcg cgtgcccctg
     2041 cgggtcggct cgcagacggt ggtggtctcc ggcgagatcg cctgggtccc cggccccgcc
     2101 ccctggccct ggatcggagc ggccgtcctg ctctgcgccg ccgtgctgct cgccgcccgc
     2161 cggggcggtg cttggcggct gcgggccctg atcgccgcca cggtcctggc ggtcgccgcc
     2221 gacgtcgtcc acgccaccgg cagctggctc ggctccaccg cctcgaccgt gaccaagctc
     2281 tacagcaacg ccacccccct cgcgggctgg gtcgtcgccg gcctcgccat ctggcggctc
     2341 ctgcgcggcc gaaccgccaa cggctccggt tcaccggacg ccctcgaaac cggcaacacc
     2401 tacctgctga tcgccggcat cttcctggcg gtcgccggcg ctctgcccga cgtcggcgtc
     2461 ctggacagct cccagatccc cgagccgttc ccgcccaacc tcacccgcgc ggtcgtcacc
     2521 atcaccctcg gcctcggctc cgccctggtc ctcgccgtcc tgctggcccc gccgacaacg
     2581 ccccgccggc ccaaaccggc gcccgacccc agcggcaacc aggccgcgcc ctcgccgtcg
     2641 ccgccccagc cttcgtaggc ggcgggccgc cagccgctca gtgccgctgg ctgaagtgat
     2701 gcgacgtctc gcggtgcgcg tcgccgccga gggtgggctg gtggccgacg tggacggtcg
     2761 cgtcggcgag ccgcggcacc tggtggagga gctgatggcg cacggcttcg gcgaggtcgt
     2821 gggcctgcgg cagtgacagg gacgcgtcgg ctgcgatctc cacctcggcg agcaggtcgt
     2881 ggccgaccca gcgcatccgg agccggtcga gggagacgac gccgggaacc tgccggatga
     2941 cctcctcggc gtggagcacg gtgtggtcgt cgacgcggtc catcatgcgc ccgtagatct
     3001 cacggaccgc ctggcggagc acgccgatga tcgcgatgcc gatggcgagc ccggcgaccg
     3061 ggtccgcggc tcgccagccc gccgccaccc cgaccgcgcc agccacgacc gccagggagg
     3121 tcagcccgtc ggtccgggcg tggaggccgt cggcgagcag cgcggccgac ccgatgcggc
     3181 ggccgacccg gatgcggtag gcggcgaccc actcgttgcc gatgaagccg acgatccccg
     3241 ccgccgcgac ccagccgagg ttgcgcaggt cgcgtgggtt caccaggtga tcgacggcct
     3301 cgtaggcggc gatcagcgcg gagagggcca tggcgagcag gacgaagagc ccggcgacgt
     3361 cctcaccccg cccgtagccg taggtgtagc ggcggttggg gaggcgacgc ccggcgagga
     3421 acgcgatccc gatcgggatg gcggtcagcg cgtcggcgaa gttgtggatc gtgtcggaca
     3481 gcagcgccac cgagcccgag gcgagcacca ccacgaactg cgccaccgcg gtggccccga
     3541 ggacgacgag cgagatcctg agggctcgca tgccctcggc actcgcggtc agtgccgagt
     3601 cgatccggtg gccggtgtcg tggctatgct ggtgaccgtg cgggtgtgga cgcaggcgcc
     3661 tgacaagctg gcggagcccg gcgccgtgga gcgcgagcac caggatgacc gccaccccga
     3721 tcacgaccgg gctgatgagc caccgccgtg cgcccaggat ggccgccctg atcaaccaga
     3781 ggtgcacggc gacggccgcc gcgccgacca cgatggtgag cgccatgccc cggcgctctc
     3841 ggagttgttc atgtagtctc atgggagcca ttctgggcgg ttgttcataa cgatgtcaag
     3901 aggtgagggc ccatgcttga caaagttatg agcgaacgcc cagaatatgc ggtgtagtta
     3961 tgagcaatcg ttcagaacag ttcgcaaagg aggtgaggcc gttgcccaga ccccttgccg
     4021 ccccggtcct ggccgccacc gacggcgact tggaggcggt ccgggagcac atcctggagg
     4081 ccacccaccg ggtcatcgac cgcaagggac tcgcggcggc gtccacccgc gccatcgctg
     4141 aggaggccgg ggtcgccggc ggcacgctct acaactactt cgacaaccac acccagctgc
     4201 tggccaaggc catcgtgtgg tatgcggcga agctcagcga tccagtcgcc agcctcccgt
     4261 cgcgtgccgg caagtacacc gtcgcccaca acctgcgcta cttcgtccgg caggccgcga
     4321 ccgtgctcga ccggctcgtc ccggccttcg cggcggcgtt ctccaacagc cagctgctgg
     4381 aggcggtgcg ccgcgagatg gccggcatcg acccgctgag cgaccccgcc cgggtggtcg
     4441 agcggtacct gctcgccgaa cgcgacctcg gccgcgtcgc cccggatgcc gagtgccgca
     4501 cggcggcctc gatcatcgtc agcctctgcc acgacgacgc ctaccagcgc tacctctacc
     4561 gggagacggg cccgccgcgg ccgcggcgcg gggagatcga cctgatcgcg cggtccctga
     4621 ccaagcccct gaccaagccc ctgaccgaat gagtgcaaag gaccggaatc atgtacctca
     4681 tcatcggaat cgtcgccgcg gccgccgtca tcgtcgccgc ggcgctgctg gcgctcgcag
     4741 tccgcgcgct gaaggccgcc cacacccggc caccagcggg agccgctgcc gctgccccgc
     4801 acacgcagga gcatgggcac ggcggcagcg ggctgcagcg aaccgttctc tcgatcgtcg
     4861 gcaggaccat cccgcggctg ctgcggctgg gtgtccgcat gggcccgatg atgctgctga
     4921 ccgtccgcgg ccgcagcacc ggcctgcccc gcaccaaccc ggtggacctg ttcgagcgtg
     4981 acggccgcca ctggctggtc gccacccacg aggccaacgc gagctgggtg cgcaacctcc
     5041 gcgccgccgg cgagggaacc ctcgcgcgag gccgccgccg ctacggcttc accgccgtcg
     5101 agctgtcaca gcgggcagcc ggaaccgtgc tcaaggaggt cctgggcccg cggctgacca
     5161 ggcccgccgc cggcttcgtg ctgcggcaga ccctcggcgt cccgccgggt gggtcgctgg
     5221 acgagttcac cagcgccgcc gcctcccatc ccgtcttcga gctcaccatc cggcgcgacg
     5281 gcgccctcgc cccgaccagc ggcatcgcat cgcaacccca acggaaggag accgcatgag
     5341 cacccccgcc accaactcga agaaagcccc catggtcgcg atcggagccg gcatcctgat
     5401 cgtcctcgcc cacctcacgc tggccgtcac cggggtcatg tcggcggcac aatggaccag
     5461 cggcgtcgtc atcggtctgc tgatcgccgg cgtcggcaac cacttcagga tcttcggacg
     5521 ccgatgagct gagatgagct gagatgagct gagagggatg ggtgcctgtg gacgtcgtcg
     5581 actccgtacg ggacttctgg gacgcggacg ccgaggggta tgaccgcgca ccggaccatg
     5641 gagccgccac cggggcggag cgggccgcgt ggagcggcgc gctggctcgg ctgctccc
//
LOCUS       DASWDC010000030         6718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1089288, whole genome shotgun sequence.
ACCESSION   DASWDC010000030 DASWDC010000000
VERSION     DASWDC010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089288"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /gene="purD"
                     /locus_tag="VF995_00885"
     CDS             <1..741
                     /gene="purD"
                     /locus_tag="VF995_00885"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX9342155.1"
                     /translation="GEAGDIILLEEFLEGPEASLFALSDGRTVVPLAPARDYKRVGDG
                     DQGPNTGGMGAYSPLPDFPEALLAEVRHTILEPTVTELSARGIPYRGLLYAGLALTPA
                     GPKVIEFNCRFGDPETQVVLPRLACELTDLLWSTAEGVLAGHKAIWDPRACVTVVLAS
                     GGYPGSYQRGLPIGGLEESAALPGVRLFHAGTASDRSGQVLTDGGRVLAVTALDDDLP
                     SARRRAYAAAELIRFEGVHYRRDIAANL"
     gene            924..1859
                     /locus_tag="VF995_00890"
     CDS             924..1859
                     /locus_tag="VF995_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342156.1"
                     /translation="MQLAREAPIPLLTGPRSLIGRAVPLAALLGGSGLAAVTVTLTFQ
                     HSLSFQSFVVLQVLLITAWVVGGEARVRRAFSAVVADRAQRLERELGYMARETVAAER
                     VRAARDLHELVTGDVEFMVSQLKAVERLLPAGPGPGSAQVREAREALGRVARGAERTV
                     ARLQRGQELLDLDGPGGCAPAHSPMSQLGALVSEVKTAGLPVDVVVEGAAVPLPAEVD
                     GSAYRIVQEALSNCLKRPGPARPQLLIRYGPQDLELQVTEDASAWHLDPDAGRHASLG
                     WVQPRPPVHDPGAEPLLEGGYGVHARLPLAATPRA"
     gene            1943..2410
                     /gene="purE"
                     /locus_tag="VF995_00895"
     CDS             1943..2410
                     /gene="purE"
                     /locus_tag="VF995_00895"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX9342157.1"
                     /translation="MPEPLVGIILGSSSDEERTQAACVMLEKFGVEFELEVLSAHRSP
                     ARVAAYAEGAADRGLQVIIAAAGKAAALPGVIAAHTVLPVIGLPIYGQQLGGQDALYS
                     IVQMPSGVPVATVGLDAAANAGILAVQILAVADPKLRAVLQDFKRQLAEGLRL"
     gene            2438..3739
                     /gene="purB"
                     /locus_tag="VF995_00900"
     CDS             2438..3739
                     /gene="purB"
                     /locus_tag="VF995_00900"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX9342158.1"
                     /translation="MIPRYTLPEMGTVWSETTRFELYAQVEAAVVRARARRGLVPAGD
                     LAVIEQAPPPDPARVHELDAQLHHDVIAFLTAYGEGIGAPARHVHYGMTSSDLIDTAL
                     ALQLARAADLLLQRLDTLVAVLRERALEHRATLALGRTHGVAAEPTTFGLKLASHAFA
                     FDRDRRRLRGARDAVAVGTISGAVGTYANLPAEVEAEVLAELSLHPEDAPTQVVARDR
                     HAQFLTALALAGANLERLATEIRHLQRTEVREAEEPFAEGQKGSSAMPHKRNPIVAER
                     LTGVARLLRGYAIAGLEDVALWHERDISHSSVERVVLPDACCVLDYGLERAAWLVGGL
                     RVNISRMRANLEASGGLAGSSAALLALVDAGWSRERAYAAVQRAAMAAWEGGCRFKEL
                     LLAEPGVAEAVGGAAGLDSALDPARFVANAEPVLKRLEELP"
     gene            3778..4665
                     /locus_tag="VF995_00905"
     CDS             3778..4665
                     /locus_tag="VF995_00905"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9342159.1"
                     /translation="MEGLRHLASGKVRDLYAVDAEQLLLVASDRVSAFDVVLPDPVPG
                     KGEVLTALTQFWLEELAGLAPGHLVGWRAAELPPGARHLAGRAMLVRRLDMVPIECVA
                     RGYLVGSGWAEYQASGTVCGIRLPAGLRQADRLEEPLFTPATKATTGHDENISEAEMA
                     ERVGGALTERLRDLTLAVYARGAEHAARRGILLADTKFEFGLDRSGQVMLADEVLTPD
                     SSRFWPAGAWEPGSSPPSFDKQFVRDWLRGQDWDKQPPAPALPPDVIAGTAARYREAY
                     ERLTGHLLDDWLAEARTGA"
     gene            4793..5239
                     /gene="mscL"
                     /locus_tag="VF995_00910"
     CDS             4793..5239
                     /gene="mscL"
                     /locus_tag="VF995_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX9342160.1"
                     /translation="MSEFKQFLLRGNVVDLAVGIVIGVAFAAVVQGMVRDLLTPLIAA
                     IFHQPDFGGLTFKFNKSVFRYGDFFNLVIAFITIALAVFFFVVKPINTLVALSRHGRT
                     LDPTTRKCPECLSEIPLKARRCMFCTVVVATSEPANQGDGVPSRTT"
     gene            5484..>6718
                     /locus_tag="VF995_00915"
     CDS             5484..>6718
                     /locus_tag="VF995_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342161.1"
                     /translation="MRATVSADGGDPDGDSSRAALSADGHVAVFASSARNLVPGPGGP
                     GAGPTHVFWRDLVHHVTRQVDVDATGSQPDGPATLPAVSADGRYVVFQSAATNLVPGG
                     TRAGADVYRRDMVAGATMLVSVSATGGEANGFSTRPSVSGDGRYVAFNSSATNLIPNA
                     PASPASGGGTQVYVRDLVAGTTVRVAPTAGGSADDVSLRPDISGDGHYLTFVSDATNL
                     VPGTTDANGERDVYRFDRLAGAVQRVSVSATGGDANGASTRPAISADGRYVVFQSNAS
                     DLISGDTNHQTDVFRRDMVTGSTIRVSVSATGAQLNAASTRGAVNTDGRYVAFASNDT
                     QVVGGDHNHSRDVFIRDLAAHTTTRVSAAPTGGDGLGCHTATTPPGTAVPLARSGAPD
                     ISTRPSLSADGHTVLFVSS"
BASE COUNT          902 a         2648 c         2223 g          945 t
ORIGIN      
        1 ggcgaggccg gcgacatcat cctgctggag gagttcctgg agggcccgga ggcgagcctg
       61 ttcgccctca gcgacggccg caccgtggtc ccccttgccc cggcgcgcga ctacaagcgc
      121 gtcggcgacg gcgaccaggg ccccaacacg ggcggcatgg gtgcctactc gccgctgccc
      181 gacttccccg aggccctgct cgccgaggtg cgccacacca tcctggagcc gaccgtcacc
      241 gagctgagcg cccgtggcat cccctaccgg gggctgctgt acgcggggct ggcgctgacc
      301 ccggcgggcc ccaaggtgat cgagttcaac tgccgcttcg gcgacccgga gacccaggtg
      361 gtgctgccgc gcctcgcctg cgagctgacc gacctgctgt ggtcgaccgc ggagggcgtg
      421 ctcgccggcc acaaggccat ctgggacccg cgcgcctgcg tcaccgtggt gctcgcctcc
      481 ggcggctacc cgggctccta ccagcgcggc ctgccgatcg gcggccttga ggagtccgcc
      541 gccctccccg gcgttcgcct gttccacgcg ggcaccgcca gcgaccggtc cgggcaggtc
      601 ctgaccgacg gcggccgggt gctcgccgtc accgccctgg acgacgacct gccgagcgcg
      661 cggcgccgcg cctacgccgc cgctgagctg atccggttcg agggggttca ctaccgccgg
      721 gacatcgcgg cgaatctctg aattaggtcg atcgtcgaag aaccgaactc cacgaatctg
      781 gtccggattc tatgccaaac tccgtagtcc tctgttcgag ggcttcgccg gagtgttaca
      841 acagcctctg agaggaacgg caagctccgt gggcccgcac ggtccagccg gctccagcga
      901 cgcacgctcg cacaggagag gccatgcaac tcgcacgcga agcaccgatc cccctgctga
      961 ccggcccccg gtccctgatc ggccgggcag tcccgctggc ggcgctgctc ggcggcagcg
     1021 ggctcgccgc cgtcaccgtc accctcacgt tccagcacag tttgtccttc cagagtttcg
     1081 tggtgctgca ggttttgttg atcaccgcct gggtggtcgg cggcgaggcg cgggtgcggc
     1141 gcgccttctc cgcggtggtc gccgaccgcg cccagcgtct cgagcgcgag ctcggctaca
     1201 tggcccggga gactgtcgcc gctgagcgtg tccgcgccgc ccgggacctg cacgagctgg
     1261 tcaccggcga cgtcgagttc atggtgtcgc agctcaaagc ggtcgagcgg ttgcttcccg
     1321 ccggccctgg gcccggttcc gcccaggtcc gggaggcccg cgaggcgctc ggccgcgtcg
     1381 cccgcggcgc cgagcgcacg gtcgcacggc tgcagcgcgg ccaggagctg ctcgacctgg
     1441 acggcccggg cggctgcgcc ccggcccaca gcccgatgag ccagctcggc gcgctggtct
     1501 ccgaggtcaa gaccgctggc ctgccggtgg acgtggtggt cgaaggcgcg gcggtcccgc
     1561 tgcccgccga ggtcgacggg tccgcctacc ggatcgtcca ggaagcgctc agcaactgcc
     1621 tcaagcgccc aggcccggca cgcccgcagc tgctgatccg ctacggcccc caggacctgg
     1681 agctgcaggt caccgaggac gccagcgcct ggcacctcga ccccgacgcc ggccgccacg
     1741 ccagcctcgg ctgggtccag ccgcgcccgc ccgtccatga tcccggtgct gagcccttgc
     1801 tggagggcgg ctacggggtc catgcccgcc tgccgctggc cgccacgccg cgtgcctgac
     1861 ccgccccctg accccggctg gttggaccgc tagaatgcac gggccgccgc tgcccgtctt
     1921 cttgactgtt cgaggagcac gtatgcctga accgctggtc gggatcatcc tcggctccag
     1981 ctccgacgag gagaggacgc aggctgcctg cgtcatgctc gaaaagttcg gggtcgagtt
     2041 cgagctggag gtgctgagcg cccaccgcag ccccgcccgg gtcgccgcct acgccgaggg
     2101 cgccgccgac cggggcctcc aggtgatcat cgccgccgcc ggcaaggccg cggccctgcc
     2161 cggcgtgatc gccgcccaca ccgtcctgcc cgtgatcggc ctgccgatct acggccagca
     2221 gctcggcggc caggacgccc tctactcgat cgtgcagatg ccatccggcg tccccgtggc
     2281 caccgtcggc ctggacgccg ccgccaacgc cggcatcctc gccgtgcaga tcctcgcggt
     2341 cgccgacccc aagctccgcg ccgtactgca ggacttcaaa cgccagctgg ccgaaggact
     2401 tcgtctttga gctggtggcg tccgctaagc ttcccgggtg atcccgcgct acacccttcc
     2461 cgagatgggg acagtctggt ctgagacgac caggttcgag ctgtatgccc aggtggaggc
     2521 tgcggtggtg cgcgcgcggg cgcgacgcgg gctggtcccg gccggcgacc tcgccgtgat
     2581 cgagcaggcc ccgccgcccg accccgcacg ggtccacgag ctggacgctc agctccacca
     2641 tgacgtgatc gccttcctga ccgcctacgg cgagggcatc ggcgcacccg ccaggcacgt
     2701 ccactacggc atgacctcct cggacctgat cgacaccgcc ctggcactgc agctggcccg
     2761 cgccgccgac ctgctgctcc agcgccttga caccctggtg gcggtgctgc gcgagcgggc
     2821 cctggagcac cgcgccaccc tggcgctcgg ccgcacccac ggggtcgccg ccgagccgac
     2881 caccttcggc ctcaagctcg ccagccacgc cttcgccttc gaccgcgacc ggcgccgcct
     2941 gcgcggcgcc cgcgacgcgg tcgcggtcgg gacgatctcg ggtgccgtcg gcacctacgc
     3001 caacctgccc gccgaggtgg aggccgaggt gctcgccgag ctcagcctcc acccggagga
     3061 cgcccccacc caagtggtgg cccgcgaccg ccacgcccag ttcctcaccg ccctggcgct
     3121 ggccggcgcg aacctcgaac ggctcgccac cgagatccgc catctgcagc gcaccgaggt
     3181 ccgcgaggcc gaggagccat tcgcggaagg tcagaagggc tcctccgcca tgccccacaa
     3241 gcgcaacccg atcgtggccg agcgcctcac cggggtggcg cggctgctgc gcggctacgc
     3301 gatcgccggc ctggaggacg tggccctgtg gcacgagcgg gacatctccc actcctcggt
     3361 ggagcgggtg gtgctgcccg acgcctgctg cgtgctcgac tacggcctgg agcgcgccgc
     3421 ctggctggtc ggcggcctgc gggtcaacat ctcgcggatg cgcgccaacc tggaggcgtc
     3481 cggcggcctg gccggctcct cggccgcgct gctcgccctg gtcgacgccg gctggtcacg
     3541 cgagcgcgcc tacgccgccg tgcagcgcgc cgccatggcc gcctgggagg gcgggtgcag
     3601 gttcaaggag ctgctgctgg ccgagcctgg cgtcgcggaa gcggtcggcg gtgccgccgg
     3661 cctggacagc gccctggatc ccgcccgctt cgtggccaac gccgaaccgg tcctcaagcg
     3721 cctggaggag ctgccgtgac ccgtccaacg ccaacctcaa cgccggcggc gccggagctg
     3781 gaagggctgc gccacctggc ctccggcaag gtccgcgacc tctacgcggt cgacgccgag
     3841 cagctgctgc tggtcgcctc cgaccgcgtc tccgccttcg acgtggtgct ccccgacccg
     3901 gtccccggca agggcgaggt gctcaccgcg ctgacccagt tctggctgga ggagctggcc
     3961 ggcctggccc ccggccatct ggtcggctgg cgcgccgccg agctgccgcc cggggcgcgc
     4021 cacctcgccg ggcgggccat gctggtccgc cgcctcgaca tggtcccgat cgagtgtgtc
     4081 gcccgcggct acctggtcgg ctccggctgg gccgaatacc aggcctcggg caccgtctgc
     4141 gggattcgtc tccccgccgg cctgcgccag gccgaccgct tggaggagcc gctgttcacc
     4201 ccggcgacca aggccaccac cggccacgac gagaacatct ccgaggccga gatggccgag
     4261 cgcgtcggcg gggccctcac cgagcgcctc cgcgacctga ccctggcggt ctatgcccgc
     4321 ggggccgagc acgccgccag gcgggggatc ctgctggccg acaccaagtt cgagttcggc
     4381 ctcgaccgca gcggccaggt catgctggcc gacgaggtcc tcacccccga ctcctcccgc
     4441 ttctggccgg ccggcgcctg ggagccgggc tcctcgccgc cctccttcga caagcagttc
     4501 gtccgcgact ggctgcgggg gcaagactgg gacaagcaac cgccggctcc ggccctcccc
     4561 cccgacgtca tcgccggcac cgccgcccgc taccgcgaag cctacgagcg cctcaccggc
     4621 cacctcctcg acgactggct cgccgaggcc cgcacgggcg cctgaggcgt ccgccaaggc
     4681 gtccgccagg cgtcgcagaa aggcgattcg ccgctcgcac cagcgggaag gcctgctctt
     4741 tgcagacgcg cctgcgagcc gcgtagatgg agggcagacg atgcgttcgc tgatgagcga
     4801 gttcaaacag ttcctactcc gaggcaacgt ggtcgacctg gcggtcggga tcgtgatcgg
     4861 ggtcgccttc gccgcggtcg tgcaggggat ggtcagggac ctgctgaccc ccctgattgc
     4921 ggcgatcttc caccagccgg acttcggcgg cctcaccttc aagttcaaca agtccgtgtt
     4981 ccgctacggc gatttcttca acctggtcat cgcgttcatc acgatcgcct tggcggtgtt
     5041 cttcttcgtg gtgaaaccga tcaataccct ggtcgcattg tcacgccacg gcaggaccct
     5101 ggacccgacc acgcgcaagt gcccggagtg cctcagcgag atcccgctga aggcgcggcg
     5161 ctgcatgttc tgcaccgtgg tggtcgcgac cagcgagccc gccaatcagg gcgatggcgt
     5221 gccgagccgt acgacgtgag catcaagcgg cttcagcgct ggaccctgca tattccgtag
     5281 agaatccctc gcgcagatag ctgagctcgg gtagagtcgc gctccggtca atcaggccgg
     5341 tcaatcggcg cgagaattcg gggggtggca tgcggcgagc aggcgcgtcc gcgcttgggg
     5401 tcgtggtcgt tggaacagcg ttgagcgtgg ttgggctctc gatgcggccc gcggcggtcc
     5461 atgcgctggg cgctggaacc accgtgcggg cgacagtgag cgccgacggc ggcgacccgg
     5521 acggcgactc gtcgcgtgcc gcactctcgg ccgacgggca cgttgcggtc ttcgcctcct
     5581 ccgcccgcaa cctcgtcccc ggtccgggcg gccccggcgc cggccccacc cacgtcttct
     5641 ggcgcgacct cgtccaccat gtcacccggc aggtggacgt cgacgccacc ggcagccagc
     5701 cggacggccc tgccaccctc cccgcggtga gcgcggacgg ccgctacgtc gtcttccagt
     5761 cggctgccac caacctggtc cccggcggca ccagggcagg cgctgatgtc taccgccgcg
     5821 acatggtcgc cggcgccacg atgctggtca gcgtcagcgc cactggcggc gaagccaacg
     5881 ggttctccac ccggccctcg gtcagcgggg acggccgcta cgtcgccttc aactccagcg
     5941 ccaccaacct gatccccaac gcgcccgcgt cacccgcttc gggcgggggc acccaggtct
     6001 acgtccgcga cctcgtggcc ggcaccaccg tccgggtcgc ccccaccgcc ggcggcagcg
     6061 ccgacgacgt cagcctccgc cccgacatca gcggggacgg tcactacctc accttcgtct
     6121 ccgacgccac caacctggtt cccggcacca ccgacgccaa cggcgagcgc gacgtctacc
     6181 gcttcgaccg cctcgccgga gccgtccagc gcgtcagcgt cagcgccacc ggcggcgacg
     6241 ccaacggcgc ctccacccgc ccagccatca gcgccgacgg ccgctacgtc gtcttccagt
     6301 ccaacgccag cgacctcatc agcggcgaca ccaaccacca gaccgacgtc ttccgccgcg
     6361 acatggtcac cggcagcacc atccgcgtca gcgtcagcgc caccggggcg cagctcaacg
     6421 cggccagcac ccgcggcgcg gtgaacaccg acggccgcta cgtcgccttc gcctccaacg
     6481 acacccaggt cgtcggtggc gaccacaacc actcccgcga cgtcttcatc cgcgacctcg
     6541 ccgcccacac caccacccgc gtcagcgccg cccccaccgg cggcgacggc ctcggctgcc
     6601 acacggccac caccccaccg ggcaccgctg ttcccctggc ccgttccggc gcccccgaca
     6661 tcagcacccg cccctccctc agcgccgacg gccacaccgt cctgttcgtc tccagctt
//
LOCUS       DASWDC010000031        12963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1506780, whole genome shotgun sequence.
ACCESSION   DASWDC010000031 DASWDC010000000
VERSION     DASWDC010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12963
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506780"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="VF995_00920"
     CDS             <1..1313
                     /locus_tag="VF995_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9342162.1"
                     /translation="RHEAMAEALHRAGPAIIASGSTVVLSLLCLLVAELQSTKSLGPV
                     MAIGIGVGLLAMLTLLPALLVIFGRWMFWPVRPALGSAEPTEHGLWARIGARLARRPR
                     TVWLVGAVALGALALGTTGLKASGIQNKDSFRTTQESVTGERALDRHFPAGAGNPVQV
                     VGNAGAAEQLRAAVQNVAGVSDVTQPRTAGGLAYIEGTLASAPDSQASYGTVDQLRTA
                     VHAIPGASGKVGGNSAVNLDIQRASHHDRNLVVPLVLVVVLVVLGLVLRALVAPLLLV
                     ATVVLSYGAALGVSALVFNHLFHFAGADTSFPLWTFVFLVALGVDYNIFLMTRVHEES
                     RQHGTRRGALIGLAATGGVITSAGLVLAGTFTALGSLPFVFITELGFAVAFGVLLDTF
                     IVRSVLVTALNLDVGRWIWWPSRLARKPDVELDELGAEAATATR"
     gene            1556..2095
                     /locus_tag="VF995_00925"
     CDS             1556..2095
                     /locus_tag="VF995_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HEX9342163.1"
                     /translation="MTQTPDHTPETPRVPSISLPRSADGANPRPTPAEAEASEQGEAV
                     ISPAKVLRIGSMVRQLLEEVRSAPLDEAGRTRMREIYDISVRELASGLSPDLAAELNR
                     VALPLEGTAPSEAELRVAQAQLVGWLEGLFHGIQAALFAQQAAARDQLAEMRGRGLPG
                     PSPGPNRDQDRSPGEGLYL"
     gene            2134..3675
                     /locus_tag="VF995_00930"
     CDS             2134..3675
                     /locus_tag="VF995_00930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX9342164.1"
                     /translation="MTNDGTLGDPRPDAGTGRLPYGPLVPRTVVSEALVTPAERGDLF
                     GGDASSDDAPVPVVIEVNLQHRDGLQRAEAELRQLLSDVLHDDDPWQVAKTYFKSRIT
                     MRQVRDLVAEDRRRHPEYQDRAIYRVWPDFPVRPLIDHSVTTVKADAAHNAYSAMGAG
                     ITWAVVDSGIDATHPHFTRYANLEHEEVAGLHRDFTDKKEPRDSALVDELGHGTHVAG
                     IIAGGLPLDWPRERLLVLRNERDENDQPVMRTRHPQRPDLLAGVAPQCRLVSLKALSA
                     DAVGDSTGVMRALEYVREINGEGKLMRIHGVNLSVGYEFKARWFACGQSPLCVEVNRL
                     VRSGVVVVIAAGNTGYGLSRADSGFTPSGLGVTINDPGNAEQGITVGATHRDMPHTYG
                     VSYFSSKGPTGDGRMKPDLVAPGENITSCAAGRKRSQMATGSAAPLAPLEELATYIDD
                     SGTSMAAPHVSGVIAAFLSIRQEFTGQPERVKQIFLDTATSLGRTEQFQGRGLVDLMR
                     AIQSV"
     gene            3727..5112
                     /locus_tag="VF995_00935"
     CDS             3727..5112
                     /locus_tag="VF995_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016890552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX9342165.1"
                     /translation="MADELAGFPWWEVAFDQAGNRTDPVADPAAIGGLATGLDLTGVT
                     DLLVMCHGWKNDRTSGRAAYEQFFTQLRGVLDDHPPRSGVSLGAMGIYWPSLLWADDR
                     AAAGPGPAAGAGPATEGDLVGELKQVYPAERQQAALDELGSLLDRQPDEQSALDRFQV
                     LLRELVPTPAGGNGASSGLGEPGDEALNLLVDGKPEEVFGVLSADSEIRAPQGAVDLG
                     SPFRALWAGAKEALRQATYWEMKNRAGTVGQRGIGPLVRALHGALPELRVHLCGHSFG
                     ARAVSFALAGLPAPGTDGATPSPVKSLTLIQGAFSHYTFATSLPFDRSRGGALAGMAA
                     RVDGPLLVTHSLADLAVGRMYPLASLVARQDAAAVDDLAERWWAMGHRGAHGVPAAEA
                     TLGAAGTAYAFTPGGFLNLDANGVIRAGGPPSGAHGDIFHPQVAWALLAGAATVEAEP
                     EPEAEGDLVGT"
     gene            5166..5885
                     /locus_tag="VF995_00940"
     CDS             5166..5885
                     /locus_tag="VF995_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HEX9342166.1"
                     /translation="MTNQDLNQNQNQGQNPEEAPLQRPAQLALLVEGGDRKGLLHDMT
                     AAIQRHDGYILSVEIVEQGARSSVYWELDGVADPESLERAFLEIDVVESVRRLPTMFQ
                     VYGKRIIVVGAGAQVGQVAIGAIGEADRHNIRGEKISVDTIPIVGEESLANAARAVAR
                     LPRAVALVLAGSLMGGEVTRAVDEVRARGILVVSLNMAGSVPAHADLVVTDPVQAGVM
                     TVMAVASTARFDIARVRGQRF"
     gene            complement(5906..6781)
                     /locus_tag="VF995_00945"
     CDS             complement(5906..6781)
                     /locus_tag="VF995_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9342167.1"
                     /translation="MAERGEAGGGPTVRRMMIGARLRRLRTDKGISREQAGEAIRASE
                     WKIHRLENGQVGFKERDIIDLLALYGVTDTAAVAQLVSLAHEANQPGWWSPYSDLLPG
                     WFRAYVDLESIATLIRTYEGQFVPGLLQTEDYMRAVIRGGHLDEVDEEVERRVDVRLR
                     RQALVRPDGPRLWAVVDEAALRRPVGGADVMRAQLERLIEAAELPNVILQVLPFSVGA
                     HPAMVGAFSILRFSEDELPDIVYLEHLTNAWYLDRRDDVEQYLHVMNSICVSAAAPDR
                     TVEILRDIAKESGGS"
     gene            6990..8135
                     /locus_tag="VF995_00950"
     CDS             6990..8135
                     /locus_tag="VF995_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX9342168.1"
                     /translation="MNPATKATATAKSAARRVDTFQERRPWLAFPYGVLRKFGEDQAG
                     NLAALIAYYGFFSIFPLLLVLVTVLGMVLRGHPAMQQRVLGSALAQFPVLGNQIQHNI
                     GSLRGGGLALIIGILLTLWSGLGVVKATETAMNSIWNVPFRQRPNFLFSTLRALLMLA
                     VLGAITLAAAIVGGFGAGGGSWLKMVAGIVLSLALNFALFLLAFRILTAAPIDWDDLW
                     IGAALGAVAWTLLQALGGYYVSHQLRSASEVYGTFAVVIGLLAWIYLGAQITLLGAEI
                     NVVRARHLWPRSLVQEAPAEHPSERSDAVHGRTAEAEAKPPTDADQRALGHYAEQEER
                     RHDEDVHVAFNHPPEAKPATRATRAGPGTETEAPAGPRRTEPERRPR"
     gene            complement(8235..8489)
                     /locus_tag="VF995_00955"
     CDS             complement(8235..8489)
                     /locus_tag="VF995_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342169.1"
                     /translation="MPIDPAALARSVSALTTLDAERDLARAIQHVTSVARGLFGSDGA
                     GLMLVDEGGALRWASASEGCPARGRASSPSSCRWRSTGGS"
     gene            complement(8710..9279)
                     /locus_tag="VF995_00960"
     CDS             complement(8710..9279)
                     /locus_tag="VF995_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342170.1"
                     /translation="MLGLALTGAGCASAQRSGAPNAPKGTSAPPSTVGGSGPSAIDAA
                     RASAKVCLHVDRHQYEVCTAYVANASYLARLPFYKFGRSADADLARTFREHLESRYTG
                     AARSLVERQTAGWPGPSQLDVFLPTIRIDSVSVAANLSTARLTTHETWLVRTEGGRTL
                     FAERDVAHVITLARVPALVLHKWVVTAIR"
     gene            9522..9719
                     /locus_tag="VF995_00965"
     CDS             9522..9719
                     /locus_tag="VF995_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342171.1"
                     /translation="MPRLEGMHAATATTIGWIEAGDSGSQCLFCGEPARHFQPLAATK
                     GPLEALKHLWWCRPCETTWVA"
     gene            9908..11113
                     /gene="fdhA"
                     /locus_tag="VF995_00970"
     CDS             9908..11113
                     /gene="fdhA"
                     /locus_tag="VF995_00970"
                     /EC_number="1.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018467 - formaldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HEX9342172.1"
                     /translation="MADSNRGVVYSGTGSVAVQDLDFPKLILAEQDNRQLNHGAILRV
                     VATNICGSDQHMVRGRTTAPVGQTLGHEITGEVIETGRDVEFIKEGDLVSVPFNIACG
                     RCRNCKERNTGVCENVNPARAGAAYGYVDMGGWLGGQARYVTIPYADWNLLKFPDRDR
                     ALAKIKDLAMLSDIFPTGYHGAYTAGVTTGSTVYVAGAGPVGLACATSCFLLGATVVI
                     VGDMNGERLAQARSFGCETIDLSRHDDLGEQIEQLLGVPEVDAAVDCVGFEARGHGQT
                     GSASEAPATVLNSLMTITRAAGRLGIPGLYVTGDPGGVDEAARVGSLSIRIGLGWAKS
                     HRFTTGQCPVMRYNRQLMMAILHDRVQIAKNVNATVIPLDQAPQGYQDFDKGAAKKFV
                     LDPHGLISN"
     gene            complement(11186..12913)
                     /locus_tag="VF995_00975"
     CDS             complement(11186..12913)
                     /locus_tag="VF995_00975"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9342173.1"
                     /translation="MAVTPERIVAGRYALERPLSRGGMGVVWLARDQLLGRGVAVKEI
                     RLPAAVPEPEREAMHARVLREARAAARLNHPGVITLYDVLNEQGHAFLVMELITAPTL
                     EAIVAERGPLPPERVARIGMQVASVLDAAHRSGIVHRDVKPANVMVAEDGGVRLADFG
                     IAQLQGDPELTASGVIVGSPAYMAPEQADGGDCGPETDLWGLGATMYYGVEGSPPFER
                     GNSIATLAAVLREPPRPPLLAGALGPVILSLLAKDPASRPALRQVRIRLARIVGADLE
                     AAAEPTAPTAPVERAMPIEPRPAEPPPAEPTLQVEALPDAADADGGDAAGETAVGGGT
                     AARRRFLALAMLAGVAAVIVVAALAGQPRSGATSGHSSGATQAHTASAPATSRSSGSA
                     SAGPRTTTAKGAAPATSPPSQPSPSPGGAVVPAGWTRFANPSGGYTVAYPSGWRRVAG
                     LARHGTAFSEGTGRYLKVESAHPPQVPANGDPVPGWIQNEQYWSARLPGYHLIGSVRG
                     GSYHGMRAAIWEYTYLLNGRPTHGLNISFVSPSRTWGYSVLSLVPADQWGASQSLIRS
                     FEQAFSPQG"
BASE COUNT         1867 a         4883 c         4418 g         1795 t
ORIGIN      
        1 accggcacga ggccatggcc gaggcgctgc accgcgcggg acccgcgatc atcgccagcg
       61 gctccaccgt ggtgctcagc ctgctgtgcc tgctggtcgc cgagctgcag tcgaccaaga
      121 gcctcggccc ggtgatggcg atcggcatcg gggtcggcct gctcgccatg ctcaccctgc
      181 tgccggcgct gctggtgatc ttcggccgct ggatgttctg gccggtgcgg ccggcgctcg
      241 gctcggccga gccgaccgag cacggcctgt gggcgcggat cggcgcccgg ctggcacggc
      301 ggccccgcac cgtctggctg gtgggcgcgg tggcgctggg tgcgctggcg ctgggcacca
      361 ccggcctcaa ggccagcggg atccagaaca aggactcctt ccgcaccacc caggagtcgg
      421 tgacgggcga gcgggcgctt gaccgccact tccccgccgg cgccggcaac cccgtccagg
      481 tcgtcggcaa cgccggcgcc gccgagcagc tgcgcgcagc cgtccagaac gtggcgggcg
      541 tcagcgacgt cacccagccg cgcaccgcgg gcggcctggc ctacatcgag gggaccctcg
      601 ccagcgcccc agactcgcag gcctcctacg gcaccgtcga ccagctccgc accgccgtcc
      661 acgccatccc aggcgcctca ggcaaggtcg gcggcaacag cgccgtcaac ctcgacatcc
      721 agcgcgcctc ccaccacgac cgcaacctgg tcgtgccgct ggtgctggtg gtggtgctgg
      781 tggtccttgg gctggtgctg cgcgcgctgg tcgcaccgct gctgctggtc gccaccgtgg
      841 tgctgtcgta tggcgcggcg ctcggggtca gcgcgctggt cttcaaccac ctgttccact
      901 tcgccggcgc cgacacgtca ttcccgctgt ggacgttcgt gttcctggtg gcgctcggcg
      961 tggactacaa catcttcctg atgacccggg tccacgagga gtcccgccag cacggcaccc
     1021 gccggggcgc gctgatcggc ctggccgcca ccggcggggt gatcacctcg gcgggcctgg
     1081 tcctggccgg caccttcacc gcgcttggct cgctgccgtt cgtgttcatc accgagctgg
     1141 gcttcgcggt ggcgttcggc gtgctgctgg acaccttcat cgtccgctcg gtgctggtca
     1201 ccgccctcaa cctcgacgtc ggccgctgga tctggtggcc cagccgcctg gcccgcaagc
     1261 ccgacgtcga gctcgacgaa ctcggcgccg aggcggcaac cgccacccgc tgagcaacag
     1321 caggagcagc gacaggaggc accccgcgcc gctggttggc gtgccgcggg gtgcctcgtc
     1381 gcggtagttc tcgtcctcct gccaatctgg gatgccgttg tcgtccctgt ccgccccttg
     1441 ccacacgctt cctgttccct cccgatcgcc ggtgattgtt cataactgtg ccggaactgc
     1501 gcgcaagcgg cagcctgcct tggctcccgg gcgcgagcgg taccatgacc gcacgatgac
     1561 ccagacaccc gatcacaccc ctgagactcc ccgcgtgccg tcgatctcgc tgccgcggag
     1621 cgccgacggc gccaaccccc gccccacgcc cgcggaggct gaggcgtccg agcagggcga
     1681 ggcggtgatc agcccggcga aggtgctgcg gatcggctcg atggtccgcc agctgctgga
     1741 agaggtccgc agcgcgccgc tcgacgaggc cggccggacc cgcatgcggg agatctacga
     1801 catctccgtc cgcgagctgg ccagcggcct ttcgccagat ctcgccgctg agctgaaccg
     1861 cgtggcgttg ccgctggagg gaaccgcccc gagcgaggcc gagctgcgcg tcgcccaggc
     1921 gcagctggtc ggctggctgg agggcctgtt ccacggcatc caggcggccc tgttcgcgca
     1981 gcaggcggcc gcccgcgacc agctcgccga gatgcgcggc cgcggcctcc ccggccccag
     2041 tcccgggccc aaccgcgacc aggatcgttc tcccggcgag ggcctctacc tctgacccgc
     2101 cgctaacccg ccgcgcgagg ggaggtgcag ccgatgacca atgacgggac ccttggcgac
     2161 cctcgtccag acgcggggac gggcaggctg ccgtatgggc cgctggtccc ccggacggtg
     2221 gtctcggagg cactggtgac cccagcggag cgcggcgacc tcttcggggg cgacgcctcc
     2281 agcgacgacg cgcccgtgcc ggtcgtcatc gaggtcaacc tgcagcaccg cgacggcctg
     2341 caacgcgccg aagcggaact ccgccagctg ctcagcgacg tgctccacga cgacgacccc
     2401 tggcaggtcg ccaagaccta cttcaagagc cggatcacga tgcggcaggt ccgcgacctc
     2461 gtcgccgagg accgccgccg gcaccccgag taccaggacc gggccatcta ccgcgtctgg
     2521 ccggacttcc cggtcaggcc gctgatcgac cactcggtca ccaccgtcaa ggccgacgcc
     2581 gcccataacg cctacagcgc catgggcgcc gggatcacct gggcggtggt cgactccggc
     2641 atcgacgcca cccaccccca cttcacgcgc tacgccaacc tggagcacga ggaggtggcc
     2701 ggcctccacc gcgacttcac cgacaagaag gagccacgcg actccgccct cgtcgacgag
     2761 ctcggtcacg gtacccacgt cgccggcatc atcgccggcg ggctgccgct ggactggccg
     2821 cgagagcggc tgctggtgct ccgcaacgag cgggacgaga acgaccagcc ggtgatgcgg
     2881 acccgccacc cccagcggcc cgacctgctc gccggggtcg cgccgcagtg tcgcctggtg
     2941 agcctcaagg cgctctccgc cgacgcggtc ggggactcca ccggcgtcat gcgcgcgctg
     3001 gagtacgtcc gcgagatcaa cggcgaaggc aagctgatgc gcatccacgg ggtcaacctc
     3061 tcggtcggct acgagttcaa ggcgcgctgg ttcgcctgcg gccagagccc cctgtgcgtg
     3121 gaggtcaacc ggctggtccg ctccggggtg gtggtggtga tcgcggccgg caacaccggc
     3181 tacggcctca gccgggcgga ttcgggcttc accccgagcg gtctcggggt gaccatcaac
     3241 gacccgggca acgccgagca gggcatcacc gtcggcgcga cccatcggga catgccccac
     3301 acctacggcg tctcctactt ctcctccaag ggcccgaccg gcgacggccg catgaaaccc
     3361 gacctggtgg cgcccggcga gaacatcacc tcctgcgcgg ctggccgcaa acgcagccag
     3421 atggccaccg gatccgccgc cccgctggca ccgctggagg agctggcaac ctacatcgac
     3481 gacagcggca ccagcatggc ggcaccgcac gtctccggcg tgatcgcggc gttcctgtcc
     3541 atccgccagg agttcaccgg ccagcccgag cgggtcaagc agatcttcct cgacaccgcc
     3601 acctcgctcg gccgtaccga gcagttccag gggcgggggc tggtcgacct gatgcgcgcc
     3661 atccagtccg tctgactgac acgagcgcga gcacgagacc gagcgcgatc gaagggaagg
     3721 aggggcatgg ccgacgagct cgccggcttc ccatggtggg aggtcgcctt cgaccaggcg
     3781 ggcaaccgca ccgaccccgt tgccgacccg gccgcgatcg gcggcctcgc caccgggctg
     3841 gacctcaccg gcgtcaccga cctgctggtg atgtgccacg gctggaagaa tgaccggacc
     3901 agcggccggg cggcgtacga gcagttcttc acccagctcc gcggcgtcct ggacgaccat
     3961 ccgccccggt ccggcgtctc cctcggcgcg atggggatct actggccctc gctgctctgg
     4021 gccgacgacc gagccgccgc cggaccaggc cccgccgccg gcgcaggccc cgcaaccgaa
     4081 ggcgacctgg tcggcgagct gaagcaggtc taccccgccg agcgccagca ggccgccctg
     4141 gacgagcttg gcagcctgct cgaccgccag cccgacgagc aatcggcgct cgatcgcttc
     4201 caggtcctgc tgcgcgagct ggtccccacc cccgccggag gcaacggcgc cagcagcggc
     4261 ctcggcgagc ccggcgacga ggccctgaac ctgctggtgg acggcaagcc cgaggaggtc
     4321 ttcggggtcc tgtcggcaga ctccgagatc cgcgcgccgc agggcgcggt ggacctcggc
     4381 tcgccgttcc gcgcgctgtg ggcgggcgcg aaggaagcgc tccggcaggc gacctactgg
     4441 gagatgaaga accgggctgg gaccgtcggc cagcgcggca tcgggccgct ggtcagagcg
     4501 ctccatggcg ccctcccgga gctgcgcgtc cacctgtgcg gccacagctt cggcgcgcgc
     4561 gccgtctcgt tcgcccttgc cggcctgccg gcgccgggca ctgacggcgc cacgccctcc
     4621 ccggtgaagt cgctgacgct catccagggc gccttctccc actacacctt cgccacctcg
     4681 ctgccgttcg accgctcgcg gggcggcgcg ctggccggca tggccgcgcg ggtggacggg
     4741 ccgctgctgg tcacccactc cctcgccgac ctggccgtcg ggcgcatgta cccgctcgcc
     4801 tcgctggttg cccgccagga cgcggcggcc gtggacgacc tcgccgagcg ctggtgggcg
     4861 atgggccacc gcggcgccca cggcgtcccc gccgccgagg cgaccctcgg cgcggccggc
     4921 accgcctacg ccttcacccc tggcggcttc ctcaacctcg acgccaacgg cgtcatccgc
     4981 gccggcgggc cgccgtcggg ggcgcacggc gacatcttcc acccgcaggt cgcctgggcc
     5041 ctgctcgccg gcgccgcgac ggtcgaggca gaacccgaac ccgaagccga aggggacctg
     5101 gtggggacct gacgccaggc gctcgggctc cgggccgtgc tcccggtcct ggtagcctct
     5161 cgcccgtgac caaccaggac ttgaaccaga accagaacca gggccagaac ccggaggagg
     5221 cgccgctgca gcgcccggcg cagctcgccc tgctggtgga gggcggcgac cgcaagggcc
     5281 tgctgcacga catgaccgcc gcgatccagc ggcacgacgg ctacatcctc tcggtggaga
     5341 tcgtcgagca gggcgcccgc agctccgtct actgggagct cgacggcgtc gccgaccccg
     5401 agagcctgga acgggcgttc ctggagatcg acgtggtcga atccgtccgg cggctgccga
     5461 ccatgttcca ggtctacggc aagcggatca tcgtggtcgg cgccggcgcc caggtcggcc
     5521 aggtcgccat cggcgccatc ggcgaggccg accgccacaa catccgcggc gagaagatca
     5581 gcgtcgacac catcccgatc gtgggcgagg agagcctggc caacgcggca cgggcggtgg
     5641 cccggctccc ccgcgcggtc gcgctggtgc tcgccgggtc gctgatgggc ggcgaggtga
     5701 ccagggccgt cgacgaggtc cgcgcccgcg gcatcctggt ggtgtcgctc aacatggccg
     5761 gctcggtccc cgcccacgcc gacctggtgg tcaccgaccc ggtccaggcc ggcgtgatga
     5821 cggtgatggc ggtggccagc accgcccgct tcgacatcgc cagagtccgc ggccagcgct
     5881 tctgacccga ccagcgacgc ggcgcctacg agccgccgct ctccttggcg atgtcgcgca
     5941 ggatctccac cgtccggtcc ggcgcggcgg cgctgacgca gatgctgttc atcacgtgga
     6001 ggtactgctc cacgtcgtcg cgccggtcca ggtaccaggc gttggtcagg tgctccaggt
     6061 agacgatgtc gggcagctcg tcctcggaga accgcaggat gctgaacgcc cctaccatcg
     6121 ccgggtgcgc ccccaccgag aagggcagca cctgcaggat cacgttcggc agctcggcgg
     6181 cttcgatcag ccgctccagc tgggcccgca tcacgtcggc gccgcccacc ggccggcgca
     6241 gcgccgcctc gtccaccacc gcccacagcc gcggcccgtc gggccgcacc agcgcctggc
     6301 ggcgcagccg cacgtcgacc cggcgctcga cctcctcgtc gacctcgtcc aggtggccgc
     6361 cgcggatcac cgcgcgcatg tagtcctcgg tctgcagcaa gccaggaacg aactggccct
     6421 cgtaggtccg gatcagcgtc gcgatcgact ccagatccac gtaggcccgg aaccagcccg
     6481 gcagcaggtc gctgtagggc gaccaccacc caggctggtt cgcttcatgc gccaggctga
     6541 cgagctgggc caccgcggcc gtgtcggtga ccccgtacag cgccagcagg tcgatgatgt
     6601 cgcgttcctt gaagccgacc tggccgtttt cgaggcggtg gatcttccac tccgacgccc
     6661 ggatggcctc gcccgcctgc tcgcggctga tccccttgtc ggtgcgcagc cgccgcagcc
     6721 gggcgccgat catcatgcgg cgcacggtcg ggccgccgcc cgcctcccca cgctcagcca
     6781 tgcgtacgcg ccttcctcgg catcaactgg gcctgccatc cgcaaattca tccggctcgc
     6841 gatgattccc gcgatccggt cggcctgagc gccgctcata ccatcccaca tggggcaccc
     6901 acgccgccag cagaggtcct caagtgtaat tgtttgcgcc gatacaggta ggggacccta
     6961 aacgctgaaa ctgcgggagg tgcgcggtca tgaatccagc caccaaggcc acagccacgg
     7021 ccaagtcggc tgcccgccgc gtggacacct tccaggagcg ccgcccctgg ctcgccttcc
     7081 cctacggcgt cctccgcaag ttcggagagg accaagcggg caacctggcg gccctgatcg
     7141 cctactacgg cttcttctcg atcttcccgc tgctgctcgt cctggtgacc gtcctcggca
     7201 tggtcctgcg cggccatccc gccatgcagc agcgcgtgct cggctccgcg ctggcccagt
     7261 tcccggtgct cggcaaccag atccagcaca acatcgggtc cctgcgcggc ggcggcctgg
     7321 cactgatcat cggcatcctg ctgacgctgt ggtcgggcct cggcgtggtc aaggcgaccg
     7381 agacggccat gaacagcatc tggaacgtgc cgttccggca acgccccaac ttcctgttct
     7441 cgaccctgcg cgcgctgctg atgctggccg tgctcggcgc catcaccctc gccgccgcga
     7501 tcgtcggcgg gttcggcgcc ggcggcggct cctggctgaa gatggtcgcc gggatcgtgc
     7561 tctcgctggc gctcaacttc gcgctcttcc tgctcgcctt ccggatcctg accgccgccc
     7621 cgatcgactg ggacgacctg tggatcggcg ccgcgctggg cgcggtggcc tggaccctcc
     7681 tgcaggcgct cggcggctac tacgtgagcc accagctccg cagcgccagc gaggtctacg
     7741 gcaccttcgc cgtggtgatc gggctgctcg cctggatcta cctcggcgcc cagatcaccc
     7801 tgctcggcgc cgagatcaac gtggtccgcg cccgccacct gtggccccgc agcctcgtcc
     7861 aggaagcgcc ggccgagcat ccaagcgagc gaagcgatgc cgtccatggg aggacggcgg
     7921 aggcggaggc gaagccgccg accgacgccg accagcgcgc cctcggccac tacgccgagc
     7981 aggaggaacg ccgccacgac gaagacgtcc acgtcgcctt caaccacccg ccggaggcga
     8041 agccggcgac gagggcgacg agggcggggc cagggacgga gacggaggcg ccggcagggc
     8101 ctcgccggac agaacctgag cggcgacctc ggtgaccgta cggcgcgacg accgcgccgt
     8161 catccgcagg cgctcgaacg cggccgcctc gccgaccccc tccctggcca tcaccatgcc
     8221 tttggcctgc tcaatcacga tccgccggtc gagcgccacc tgcagctgct cggcgagctg
     8281 gctctgcctc tggctggaca cccctcggac gcgctcgccc agcgcagcgc cccgccctcg
     8341 tcgaccagca tcagcccggc gccgtcgctg ccgaacaagc ccctggcgac gctggtgacg
     8401 tgctggatcg cccgtgccag gtcgcgctcg gcgtcaagcg tggtcagcgc gctgacgctc
     8461 cgcgccagcg ctgccggatc gatcggcatg cgatcctctc tcgcatcacc aacgacccgg
     8521 gcagggtcca ggcccggcgg aacctggcac cggcgcctca gcgcggggcg gtgccagtcg
     8581 gcggccggag tcgccgccat gccagccagg caggcagcgc ccggcacgtc acatttcacg
     8641 taccaggcgg tcaaatggtt agttatagaa ctgccctggc ggctggcctc cgccgccagg
     8701 cggacgggat caccggatcg cggtgaccac ccacttgtgg agcaccagcg cgggaacccg
     8761 agccagcgtg atcacgtgcg cgacgtcgcg ctcggcgaac aacgtccggc caccctcggt
     8821 ccggaccagc caggtctcgt gggtcgtcag cctcgcggtc gaaaggttcg cggcgactga
     8881 aaccgagtcg atacggatcg tcggcaggaa cacgtccagc tggctcggcc ccggccagcc
     8941 cgccgtctga cgctcgacca gcgaccgggc agcacccgtg tagcggctct ccaggtgctc
     9001 tctgaacgtc cgcgccaggt cggcgtcggc gctgcgcccg aacttgtaga acggcagccg
     9061 cgccaggtag ctggcattgg ccacatacgc ggtgcagacc tcgtactggt gccggtcgac
     9121 gtgcaggcag accttcgccg acgcccgggc ggcgtcaatc gccgacgggc cgctcccccc
     9181 aaccgtcgac ggcggcgccg aggtgccctt gggcgcgttc ggtgcaccgc tccgctgcgc
     9241 gctcgcacac ccggctcccg tcagcgccag ccccaacacg atcgccatca gggggatggc
     9301 tccacgcctc atcacttcgc tcctcccggc agggctcgtc gtcctgcctc gtcacgaggg
     9361 ttcttccctt caaccgtaag ccctccagcg ccgctcgcta cggcgacggt cgcaactggt
     9421 tcgtcacatc gcgacaacat cgccgcaaca caagctggat accgtcaaag caggctgcgg
     9481 accggccgcg gagcaccgag cggagcacca acatgaccag catgcccagg cttgagggga
     9541 tgcacgccgc caccgccacc accatcgggt ggatcgaggc cggcgacagc ggttctcaat
     9601 gcctgttctg cggcgagcca gcacgccact tccagccgct ggccgccacc aaaggccccc
     9661 tggaggcgct caagcacctc tggtggtgcc gcccctgcga gaccacctgg gtcgcctgaa
     9721 acccacccgt ccgcgcagct tgctcgcttg actccagaac ccagcggagt aggttggccc
     9781 attgggatct gcgtcggggg tgatggcgat gcgcattcga cgatcggccc gccgcgcacg
     9841 cggccctccc cgacccccca ccgcaacgcc gcccagaccc ctcgttcttc ctcaggaggg
     9901 aagccccatg gcggacagca accgtggcgt cgtctacagc ggcaccggat cagtcgcggt
     9961 ccaggacctc gacttcccta agctcatcct cgccgagcag gacaaccgcc agctcaacca
    10021 tggcgccatc ctgcgcgtcg tcgccaccaa catctgtggg tctgaccagc acatggtccg
    10081 aggccgtacc accgccccgg tgggccagac cctcggccac gagatcaccg gcgaggtgat
    10141 cgagaccggc cgcgacgtcg aattcatcaa ggagggcgac ctggtcagcg tccccttcaa
    10201 catcgcctgc ggccgctgcc gcaactgcaa ggaacgcaac accggcgtct gcgagaacgt
    10261 caaccccgcc cgcgccggcg ccgcctacgg ctacgtcgac atgggcggct ggctcggcgg
    10321 ccaggcccgc tacgtgacca tcccctacgc cgactggaat ctgctcaagt tccccgaccg
    10381 cgaccgggcc ctggccaaga tcaaagacct ggccatgctc tccgacatct tcccgacggg
    10441 gtaccacggc gcgtacaccg ctggcgtcac cacgggctcg accgtgtacg tcgccggtgc
    10501 aggccccgtg ggccttgcct gtgccacctc gtgcttcctg ctgggggcga cggtggtgat
    10561 cgtcggggac atgaacggcg agcggctggc gcaggcccgc agcttcggct gcgagaccat
    10621 cgacctgtcg cggcatgacg acctcggcga gcagatcgag cagctcctcg gggttcccga
    10681 ggtcgacgcg gcggtcgact gcgtcgggtt cgaggcgagg ggccacggcc agaccggctc
    10741 agcctccgag gcgccggcga cggtgctgaa ctcgctgatg acgatcaccc gcgccgctgg
    10801 ccggctcggc atccccggcc tgtacgtgac cggcgacccg ggcggggtgg acgaggccgc
    10861 ccgggtcggt tcgctgtcga tccgcatcgg cctcggctgg gcgaagtccc accgcttcac
    10921 caccggccag tgcccggtga tgagatacaa ccgacagctg atgatggcca tccttcacga
    10981 ccgcgtgcag atcgcgaaga acgtcaacgc caccgtcatc ccgctcgacc aggcgcccca
    11041 gggctatcag gacttcgaca agggagcggc caagaagttc gtcctcgatc cgcacggcct
    11101 gatctccaac tgaccaagac cagcacgccg caaccgtggg gaggggccgg gagagatcct
    11161 ggctcctccc gcgctccgtg cctggttagc cctgcgggga aaatgcctgc tcgaaggagc
    11221 ggatcaggct ctgggacgct ccccactggt ccgctgggac gagggagagg accgaatagc
    11281 cccaggtgcg gctcgggctc acgaagctga tgttgagccc gtgggtgggg cggccgttca
    11341 gcaggtaggt gtattcccag atggcggcgc gcatgccgtg gtagctgccc ccgcggaccg
    11401 agccgatcag gtggtagccg ggcagcctcg ccgaccagta ctgctcgttc tggatccagc
    11461 cggggaccgg atcgccgttc gccgggactt gtggcgggtg ggcggactcc accttgaggt
    11521 agcggccggt gccctcgctg aacgccgtgc cgtggcgggc caggccggcg acgcgccgcc
    11581 agccggacgg gtaggcgacc gtgtagccgc cgctcgggtt ggcgaagcgg gtccagcccg
    11641 ccggcacgac ggcgccgcct gggctgggcg agggctgcga tggcggggag gtggccggcg
    11701 cggcgccctt cgcggtcgtg gtccgtgggc cagccgaggc tgagccgctg gagcggctgg
    11761 tcgctggcgc gctggccgtg tgagcctgcg tcgctccgga cgagtgtccg ctggtggcgc
    11821 cgctccgcgg ctggccagcc agcgcggcca ccacgatgac cgctgcgacc ccggcgagca
    11881 tggcgagggc gaggaaccgg cgccgtgccg ccgtgccgcc gccgacggcc gtctccccgg
    11941 cggcatcccc accgtcggca tcggcagcgt ctgggagcgc ttcgacctgg agggtcggct
    12001 cggccggcgg gggttccgcg ggccgcggct cgatgggcat ggcgcgctcg acgggcgcgg
    12061 tgggcgcggt gggctccgca gccgcctcca ggtcggcgcc gacgatccgg gcgaggcgga
    12121 tacggacctg gcgcagggcc ggccgggaag ccgggtcttt ggccagcagc gacaggatga
    12181 cgggcccaag cgcgccggcg agcagcggcg gccggggcgg ctcccgcagc accgcggcca
    12241 gggtggcgat cgagttgccc cgctcgaacg gagggctgcc ctcgaccccg tagtacatgg
    12301 tcgcacccag cccccacagg tcggtctccg ggccgcagtc gccgccgtcc gcctgctcgg
    12361 gggccatgta ggcgggtgag ccgacgatca cgccgctggc ggtgagctcc gggtcgcctt
    12421 ggagctgggc gatgccgaag tcggcgagcc ggacgccgcc atcctcggcc accatgacgt
    12481 tggcgggctt gacgtcgcga tgcacgatcc cgctccggtg cgcggcgtcg agcaccgagg
    12541 cgacctgcat gccgatgcgg gcgacccgct ccggcggcag cggcccccgc tccgcgacga
    12601 tcgcctccag ggtgggggcc gtgatcagct ccatcacgag gaaggcatgg ccctgctcgt
    12661 tgagcacgtc gtagagggtg atgacgccgg gatggttgag ccgcgccgcg gcccgcgcct
    12721 cgcggagcac cctggcgtgc atcgcctcgc gctccggctc gggaacggcg gcgggaagcc
    12781 ggatctcctt gacggcgacc ccgcgaccaa gcagctggtc gcgggcgagc cagaccaccc
    12841 ccatgccgcc cctgctgaga gggcgctcca gtgcgtagcg gcccgcgacg atccgctcag
    12901 gcgtgacggc catagcctca actacccggc gaggcgatgg acccaaacct caacggggag
    12961 gtg
//
LOCUS       DASWDC010000032         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_755261, whole genome shotgun sequence.
ACCESSION   DASWDC010000032 DASWDC010000000
VERSION     DASWDC010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755261"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1304)
                     /locus_tag="VF995_00980"
     CDS             complement(45..1304)
                     /locus_tag="VF995_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX9342174.1"
                     /translation="MSDSKPRAGSPVVAIALPRAAAARAVVAAWERGEAVAPLDPQAP
                     APERARALAALRPTHLEDERGRVALEGGVPAAPGVAAVVATSGTSGTPKAVELTADGI
                     RASALAVSEALGAGGGELWLCCLPLHGVAGLAIIARAWHTGVPVQVHERFEPDRVLRA
                     ARDDGATLVSLVPTMLARLLDLDPAAAALARFRRVLLGGAPAPAALLERAAAAGLQMA
                     TTYGLTETFGGVVHDGHPLRGVELRLDEAGQILVRGPMVMRGYRAAPGGQQTTTALDP
                     DGWLRTGDLGRFEQDPSGAPGRLWVLGRLDDLIISGGVNVQPAEVEAVLATHPAVADA
                     AVRGAPDPEWGERVVAYVVCGDPAAPPSLDELRAFARERLAAAKLPRELVLVREIPRS
                     PGGKTLRRLLGRSTPPPAPPSARSGCR"
     gene            1374..>2162
                     /gene="menB"
                     /locus_tag="VF995_00985"
     CDS             1374..>2162
                     /gene="menB"
                     /locus_tag="VF995_00985"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX9342175.1"
                     /translation="MDWQRAGDYGDIRYETAEGIAKITICRPEVRNAFRPKTLFELSD
                     AFNAARDDPGIGAIILTGEGPEAFCSGGDQRIRGDDGYIDDHGVGRLNVLDLQIQIRR
                     LPKPVVAMVAGYAIGGGHVLHLVCDLTIAADNARFGQTGPRVGSFDAGYGMGMLARTV
                     GRKKAAEVWFLCEQYDAQEALRMGLVNKVVPLERLEEETVEWCRRMLRHSPLALRLLK
                     AGLNADTDGLAGIQQLAGDATLLYYLSEEAQEGRDAYREKRRPDF"
BASE COUNT          312 a          767 c          828 g          255 t
ORIGIN      
        1 gttcccgctc tggtcgctgc tggtggtcgc gctggacatc ctggtcatct acaacctgac
       61 cgcgcactgg gaggagcagg cggcggagta gaccgcccca gcagccgccg cagggtcttc
      121 ccgccgggac tgcgggggat ctcgcggacc agcaccagct cgcgcgggag cttggcggcg
      181 gccaggcgct cgcgggcgaa ggcgcgcagc tcgtcgaggc tgggcggcgc ggcggggtcg
      241 ccacagacga cgtaggcgac cacccgctcg ccccactcag ggtcgggggc gccgcgcacg
      301 gcggcgtcgg cgaccgccgg gtgggtcgcc agcaccgcct cgacctcggc gggctggacg
      361 ttcaccccgc cgctgatgat caggtcgtcg aggcggccga ggacccacag ccgtcccggc
      421 gcccctgacg ggtcctgttc gaagcgtccg aggtcgccgg tgcggagcca gccgtcggga
      481 tcgagggcgg tggtggtctg ctggccgccg ggagcggcgc ggtagccgcg catcaccatc
      541 ggcccgcgca ccaggatctg acccgcctcg tcgaggcgca actccacgcc gcgcagggga
      601 tggccgtcgt ggaccacgcc gccgaaggtc tcggtcagcc cgtaggtggt ggccatctgg
      661 aggccggccg ccgccgcgcg ctccagcagc gccgccgggg cgggggcgcc gccgagcagg
      721 acgcgccgga agcgggccag cgccgccgcc gccgggtcca ggtcgagcag gcgcgcgagc
      781 atggtgggga ccagcgacac cagggtggcg ccgtcgtcgc gggcggcgcg cagcacccga
      841 tcgggctcga agcgctcatg gacctggacg gggacgccgg tgtgccaggc acgggcgatg
      901 atcgccagcc ccgcgacccc gtggagcggc aggcagcaca gccagagctc gccgccgccg
      961 gcgccgaggg cctcggaaac cgccagcgcg gaggcgcgga tgccgtcggc ggtgagctcc
     1021 accgccttgg gcgtgccgct ggtgccggag gtggcgacca cggcggccac ccccggcgcc
     1081 gccgggacac cgccctccag cgccacccgc ccacgctcgt cctccagatg ggtggggcga
     1141 agcgccgcca gcgcacgggc ccgctccggc gcgggcgcct gcgggtcgag cggcgcgacc
     1201 gcctcgcccc gctcccaggc agccacgacc gctctggcag ctgctgctct gggcagggcg
     1261 atcgccacca ccggggagcc tgctcggggc ttggaatcgg acacgaggag taggctacgg
     1321 cacgtcgacg attgcatcac caagcactga ttgatcaagg agcaggcagc cgcatggact
     1381 ggcagcgagc cggcgactac ggcgacatcc ggtacgagac cgccgagggc atcgccaaga
     1441 tcaccatctg ccggccggag gtacgcaacg ccttccggcc gaagacgctg tttgagctgt
     1501 cggatgcgtt caacgccgct agggacgatc cgggcatcgg ggcgatcatc ctcactggcg
     1561 aggggccgga ggccttctgc tccgggggcg accagcgtat ccgcggcgac gacggctata
     1621 tcgacgacca cggcgtcggg cggctcaacg tcctcgacct ccagatccag atccggcggc
     1681 tgccgaaacc ggtggtcgcc atggtggccg gctacgccat cggcggcggg cacgtcctgc
     1741 acctggtctg cgacctgacg atcgcggcgg acaacgcccg cttcgggcag accggcccgc
     1801 gggtcggctc gttcgacgcc ggttacggca tggggatgct ggcccgcacc gtcgggcgca
     1861 agaaggccgc cgaggtctgg ttcctgtgcg agcagtacga cgcccaggag gcgctgcgga
     1921 tggggctggt caacaaggtc gtgccgctgg aacggctgga ggaggagacg gtcgagtggt
     1981 gccgtaggat gctgcggcac agcccgctgg cgctgcggct gctgaaggcg gggctcaacg
     2041 ccgacaccga cgggctcgcc ggcatccagc agctggccgg cgacgcgacc ctcctctact
     2101 acctgagcga ggaggcgcag gagggacgcg atgcctaccg ggagaagcgg cggccggact
     2161 tc
//
LOCUS       DASWDC010000033         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1089686, whole genome shotgun sequence.
ACCESSION   DASWDC010000033 DASWDC010000000
VERSION     DASWDC010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089686"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VF995_00990"
     CDS             complement(<1..851)
                     /locus_tag="VF995_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HEX9342176.1"
                     /translation="MSARAAGALGARGRWRQTVWGELSAEFLGTFVLIAFGDGVVAMA
                     VAALNQSGRGSQIFAASGDWLLITWGWAMAVTFGVYVAGGVSGAHINPAVTLALAVRR
                     AFPWAKVLPYIAAQVVGAFAGAALVYLNYHNAIGSYETAHHITRGALGGAADSTATFS
                     IFATFPAPFFHASMIWPLVDQVIGTAFLVMFVFALTDERNQPPRMNLAPLLVGLAVAA
                     IGMSYGANAGYAINPARDFGPRLLAFFTGWGKVALPGVHDYFWIPIVGPFVGAVVGAF
                     VYDLFIR"
     gene            complement(905..1036)
                     /locus_tag="VF995_00995"
     CDS             complement(905..1036)
                     /locus_tag="VF995_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342177.1"
                     /translation="MEAVFWFVFGGVVGMAVLPHLQRLAARVRRELAQLDYPKRPNS"
     gene            complement(1141..>2228)
                     /locus_tag="VF995_01000"
     CDS             complement(1141..>2228)
                     /locus_tag="VF995_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342178.1"
                     /translation="MLVASLATALVYGWGGWLAVRGALDVGTVVALTSYLIRLYGPLT
                     ALSNVNVDVMTALVSFERVFEVLDLEPMVADKPGAEALPPDAATIEFDQVDFRYPMAE
                     EVSLASLESVAKLDASIDKQVLHEVSFRADAGELIALVGPSGAGKTTISQLVTRMYDA
                     TGGAVLLGGRDVRNVTLASLRHAVGVVTQDAHMFHDSIRANLLYARPDATEPELWEAL
                     YAAQLGALVALLPDGLDTVVGDRGYRLSGGEKQRLAIARLLLKAPSIVVLDEATAHLD
                     SESEVAVQRALKTALAGRTSLVIAHRLSTVREADRILVIDDGRVVERGRHDELLAGGG
                     LYAELYRTQFERQDQGTAQRENEGLRA"
BASE COUNT          355 a          762 c          816 g          295 t
ORIGIN      
        1 tcgcggatga agaggtcgta gacgaacgcg ccgaccacgg cgccgacgaa cggccccacg
       61 atcgggatcc agaagtagtc gtggacgccg gggagggcga ccttgcccca gccggtgaag
      121 aacgccagca gccgcgggcc gaagtcgcgg gcggggttga tggcgtagcc ggcgttggca
      181 ccgtaggaca tgccgatcgc ggcgaccgcc aggcccacca gcagcggggc caggttcatg
      241 cgcggcggct ggttgcgctc gtcggtcagg gcgaacacga acatcaccag gaaggcggtg
      301 ccgatcacct ggtcgaccag cggccagatc atgctcgcgt ggaagaacgg cgcggggaag
      361 gtcgcgaaga tgctgaaggt cgcggtcgag tcggcggcgc cgcccagggc cccgcgggtg
      421 atgtggtggg cggtctcgta gctgccgatg gcgttgtggt agttcagata gaccagcgcc
      481 gcgccggcga aggcgcccac cacctgggcg gcgatatagg gcagcacctt ggcccaggga
      541 aaggcgcgcc gtaccgccag cgccagggtc accgccgggt tgatgtgggc gccgctgacc
      601 ccgccggcca cgtagacgcc gaaggtgacc gccatggccc agccccaggt gatcagcagc
      661 cagtcgccgc tggcagcgaa gatctggctg ccccgcccgg actggttgag cgcggcgacc
      721 gccatcgcca ccacgccgtc gccgaaggcg atcagcacga aggttcccag gaattcggct
      781 gacagctccc cccacacggt ttgccgccag cgcccgcgtg cgccgagcgc accagccgca
      841 cgagctgaca tcctcgtcac tccttttcga gcgagagcgc caccgagcgc gctgctcgcc
      901 acggtcacga gttgggacgc ttgggatagt cgagctgtgc cagctcccgc cgcaccctcg
      961 cggcgaggcg ctggagatgg ggcagcacgg ccatgccgac gacccctccg aagacgaacc
     1021 agaagacggc ttccatgccc cttcccccac cacgcggtag gcacgcgcat ttcgcgtcgc
     1081 atttcgcgtc gcaatccgag tcctacccag ccgcgggggt ggtcgaaacc gctgatcagg
     1141 tcaagcccgc aggccctcgt tttcgcgttg agccgtgccc tggtcctgcc gctcgaactg
     1201 ggtgcggtac agctcggcgt agaggccgcc gccggccagc agctcgtcgt ggcggccgcg
     1261 ctcgaccacg cggccgtcgt cgatcaccag gatccggtcg gcctcgcgga cggtcgacag
     1321 ccggtgggcg atcaccagcg aggtgcggcc ggccagggcg gtcttgagcg cccgctggac
     1381 cgcgacctcg gactcggagt ccaggtgggc ggtggcctcg tcgagcacga cgatgctggg
     1441 ggccttgagc agcagccggg cgatcgccag gcgctgcttc tcgccgccgg agaggcggta
     1501 gccgcggtcg ccgacgacgg tgtcgagccc gtcgggcagc agcgcgacca gcgcgccgag
     1561 ctgcgccgcg tacagcgcct cccacagctc cggctcggtc gcgtcggggc gggcgtagag
     1621 gaggttcgcg cggatgctgt cgtggaacat gtgcgcgtcc tgggtgacga ccccgacggc
     1681 gtggcgcagc gaggcgaggg tgacgttacg cacgtcgcgg ccgccgagca ggacagcgcc
     1741 gccggtggcg tcgtacatgc gggtgaccag ctggctgatg gtggtcttgc cagcgccaga
     1801 ggggccgacc agcgcgatca gctcgccagc gtcggcgcgg aagctgacct cgtgcaggac
     1861 ctgcttgtcg atactggcgt cgagcttggc gacgctctcc agcgaggcca gcgagacctc
     1921 ctcggccatg gggtagcgga agtcgacctg gtcgaactcg atggtggcgg cgtcgggtgg
     1981 cagcgcctcc gcgccgggct tgtcggccac catcggctcc aggtcgagca cctcgaagac
     2041 ccgctcgaag gagaccaggg cggtcatcac gtcgacgttg acgttcgaga gcgccgtcag
     2101 cggcccgtac aggcggatca ggtaggaggt cagcgccacc acggtgccga cgtcgagcgc
     2161 gccgcgcacc gccagccagc cgccccagcc gtagaccagc gcggtggcga gcgaggcgac
     2221 cagcatca
//
LOCUS       DASWDC010000034         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725 k141_7993,
            whole genome shotgun sequence.
ACCESSION   DASWDC010000034 DASWDC010000000
VERSION     DASWDC010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7993"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VF995_01005"
     CDS             complement(<1..1250)
                     /locus_tag="VF995_01005"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9342179.1"
                     /translation="MSVKGTRIPEHLRLPFAGVTPAPTEAEGQKLFPTGNFRFFRPVY
                     RDKMPPCNNACPTGEQIQKYLDFVKHERFLDGYLTILEDNPMPAVTGRVCYHPCESAC
                     NRKEHDEPIGIRNVERYLGDFGLALAEDPIGPALPPRNGGHVAVVGSGPGGLACAYHL
                     RRLGHQVTILEARSKPGGMLRGGIPHWHLPEEVLNAEIAKIERMDGVEIRCGTRVGTD
                     VSWEDLGRYDAAFLAVGQDVGRRLPVDGGDLRGVMGGLEFLREGGYGRPVRTGRKVLV
                     IGGGNTASDSARSALRLGADEVAIVSLEAPEELLIVPEDLDQARDEGVAFRTNTACVR
                     ILGEDGVVTGAVLARARLAKDEHGTVTPEIAPGTEFEVECDTVMFAIGQGQNLDWVPD
                     HLKNARGLLESDAHGRLEGLPGKV"
     gene            complement(1298..>1668)
                     /locus_tag="VF995_01010"
     CDS             complement(1298..>1668)
                     /locus_tag="VF995_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342180.1"
                     /translation="SARDPEYLLRFLSEPLRAKLDRLVVVDAVGLAEQQGTSPWMFVR
                     DLGQLAYDRMSTEGASERSEVGIGIAAPLIGALTAVTGVLPLDTVAQTVFDQAAFRRG
                     AERHTVLDYAERHAATATAT"
BASE COUNT          207 a          588 c          618 g          255 t
ORIGIN      
        1 aagaccttgc ctggcaggcc ttccagccgc ccgtgggcgt cggactcgag cagccctcgc
       61 gcgttcttca ggtggtccgg cacccagtcc aggttctggc cttggccgat ggcgaacatc
      121 accgtgtcgc actcgacctc gaattcggtg cccggggcga tttcgggggt gacggtgccg
      181 tgctcgtcct tggcgaggcg cgcccgcgcc agcaccgcgc cggtgaccac gccgtcctca
      241 ccgaggatgc gcacgcaggc ggtgttggtg cggaacgcca cgccctcgtc gcgggcctgg
      301 tccaggtcct cggggacgat cagcagctcc tcgggcgcct ccaggctcac gatggcgacc
      361 tcgtcggcgc caaggcgcag ggcggagcgg gcgctgtcgg aggcggtgtt gccgccgccg
      421 atcaccagca ccttccggcc ggtccgcacc gggcggccgt agccgccctc gcgcaggaac
      481 tcaaggccgc ccatgacgcc gcgcaggtcg ccgccgtcga cggggaggcg cctgccgacg
      541 tcctggccga ccgcgaggaa ggcggcgtcg tagcggccga gatcctccca gctgacgtcg
      601 gtgccgacgc gggtgccgca gcggatctcg acgccgtcca tgcgctcgat cttggcgatc
      661 tcggcgttca gcacctcctc cggcaggtgc cagtggggga tgccgccgcg cagcatcccg
      721 ccgggcttgc tccgcgcctc caggatcgtg acctggtggc cgaggcggcg caggtggtag
      781 gcgcaggcga gcccgccggg cccggagccg accacggcga cgtgcccgcc gttgcggggc
      841 ggcagcgccg ggccgatcgg gtcctccgcc agcgccagcc cgaagtcgcc gaggtagcgc
      901 tcgacgttgc ggatgccgat cggctcgtcg tgctccttgc ggttgcaggc gctctcgcac
      961 gggtggtagc agacccggcc cgtgaccgcg ggcatcgggt tgtcctcgag gatcgtcagg
     1021 tacccgtcca ggaagcgctc gtgcttgacg aagtcgaggt acttctggat ctgctcgccc
     1081 gtggggcagg cgttgttgca tggcggcatc ttgtcgcggt agacgggccg gaagaagcgg
     1141 aagttcccgg tcgggaacag cttctggccc tccgcctcgg tgggtgccgg cgtgaccccg
     1201 gcgaagggga gccgcaggtg ctcggggatc cttgtgccct tgactgacat acgcgtccct
     1261 cgaaggtcgg tggcgggcgt cgctcggggc tctgctgcta ggtcgcggtg gccgtcgcgg
     1321 cgtgtcgctc ggcgtagtcc agaactgtgt gccgctcggc cccgcgccgg aaggcggcct
     1381 ggtcgaagac cgtctgcgcc accgtgtcca gcggcaacac gccggtgacg gcggtcaggg
     1441 ccccgatcag cggcgccgcg atgccgatgc cgacctcgct gcgctcgctg gcgccctcgg
     1501 tcgacatgcg gtcgtaggcg agctgcccca ggtcgcggac gaacatccag gggctcgtcc
     1561 cctgctgctc ggccaggccg accgcgtcga ccaccaccag cctgtccagc ttggcgcgca
     1621 gcggctcgga caggaagcgc agcaggtact cggggtcgcg ggccgagt
//
LOCUS       DASWDC010000035         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1009912, whole genome shotgun sequence.
ACCESSION   DASWDC010000035 DASWDC010000000
VERSION     DASWDC010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009912"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VF995_01015"
     CDS             complement(<1..918)
                     /locus_tag="VF995_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX9342181.1"
                     /translation="MVAGSSSIPAPAPAATAVALGEAMLRLSPPGRVRLEQARSLEVH
                     VGGAELNVLVVLAQLGLRARWVTGLPDNPLGRLIERHARAWGVEVAARRETGGRAGLY
                     FVEHGAPPRPSEVLYDRANSTAARLVPGQLDWDALLTGAACLHVSGITSGLGEGPHKA
                     ALEGLATARERGVTTSFDVNFRSRLWSPRDARARLLEVLPSTEVLFASAHGLELLLGE
                     PLAGGGGAGAPLDVAVDGARRLRDDYGIGTVVLSGREDASHDTVRTSATAVTDGGRVV
                     RSGPCDAQVVDAFGAGDAGAAGFLAAYLAG"
     gene            complement(922..1713)
                     /locus_tag="VF995_01020"
     CDS             complement(922..1713)
                     /locus_tag="VF995_01020"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9342182.1"
                     /translation="MKLVSFPGGFGRLDGDVVVPMGPSLVGYLDGAAVAEGEPKPLDQ
                     VRLLAPVPSPGKVICVGLNYRDHAAETGAAIPDEPILFPKWANSVVGPGAPVEVPAAA
                     GNIDYEAELAVVIGRRAREVGAADALSFVAGYACANDVSSRELQFHSSQWLLGKAIDS
                     FLPLGPFMATADEVPDPQKLGIRCLVNGEVRQDSSTDQMIFGVAELVAFVSRTLTLQP
                     GDVIATGTPPGVGMARQPPVWLRPGDEVTIEIVGLGALTNPIRSR"
     gene            complement(1817..>2171)
                     /locus_tag="VF995_01025"
     CDS             complement(1817..>2171)
                     /locus_tag="VF995_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEX9342183.1"
                     /translation="GQDRFWFMWDDLVRGAIGAVVLVDTRRLADCFAAVDYFEQRELP
                     FIVAINCFNGLLAHAVEDVRDALAVGEDTPIVLCDARSRESAKAGLIELVQHALARAS
                     ATLSGGLPARSGTPR"
BASE COUNT          328 a          794 c          789 g          260 t
ORIGIN      
        1 gccggcgagg taggcggcca ggaagccggc agcgccggcg tcgcccgcgc cgaaggcgtc
       61 gacgacctgg gcatcgcatg gcccgctccg cacgaccctg ccgccgtcgg tcaccgcggt
      121 cgccgaggtg cggacggtgt catggctggc gtcctcgcgc ccggagagaa ccaccgtgcc
      181 gatgccgtag tcgtcgcgca gccgcctggc gccgtccacc gccacgtcca gcggagcgcc
      241 cgcgccgccg cctccggcga gtggttcgcc gagcagcaat tcaaggccgt gcgcggaggc
      301 gaacagcacc tcggtggagg gcagcacctc cagcaggcgg gcccgcgcgt cccgcggcga
      361 ccagagccgc gagcggaagt tcacgtcgaa gctggtggtc acgccacgtt cgcgggcggt
      421 ggcgaggccc tccagcgccg ccttgtgcgg gccttcgccg aggccgctgg tgatgccgga
      481 gacgtgcagg caggcggctc cggtgagcag ggcgtcccag tcgagctggc cggggacgag
      541 ccgggcggcg gtggagttgg cgcggtcgta gagcacctcc gacggtcgcg gtggcgcgcc
      601 atgctcgacg aagtagagcc cggcacggcc gccggtctcc cggcgcgccg cgacctccac
      661 gccccaggcg cgggcgtggc gttcgatcag ccggccgagc gggttgtccg gaaggccggt
      721 cacccagcgc gcccgcaggc cgagctgcgc caggacgacc agcacgttca gctccgcgcc
      781 gccgacgtgg acctccaggc tgcgggcctg ctccaggcgg acgcggccgg gcggcgacag
      841 ccgcaacatc gcctcgccca gggcgaccgc ggtcgcagcc ggtgccggtg ccggtatcga
      901 ggaggagccc gcgaccaccg ctcaccgact ccggatcggg ttggtgagcg cgccgaggcc
      961 gacgatctcg atggtcacct cgtcgccggg ccgcagccag acgggcggct ggcgggccat
     1021 gccgacgccc ggcggggtgc cggtggcgat cacgtcgcct ggctgcaggg tgagggtccg
     1081 gctgacgaag gcgaccagct cggcgacgcc gaagatcatc tggtcggtgc tggagtcctg
     1141 gcggacctcg ccgttgacca ggcagcggat gccgagcttc tgcgggtcgg ggacctcgtc
     1201 ggcggtggcc atgaagggcc cgagcggcag gaagctgtcg atcgccttgc cgagcagcca
     1261 ctggctggag tggaactgca gctcacggga ggagacgtcg ttggcgcagg cgtacccggc
     1321 gacgaaggag agtgcgtcgg cagcgccgac ctcgcgggcg cggcggccga tgaccacggc
     1381 cagctcggcc tcgtagtcga tgttgccggc ggcggcgggg acctcgaccg gcgcgcccgg
     1441 cccgaccacc gagttggccc acttggggaa caggatcggc tcgtccggga tggccgcgcc
     1501 ggtctcggcg gcgtggtctc ggtagttcag cccgacgcag atcaccttgc cggggctggg
     1561 caccggcgcc agcaggcgca cctggtcaag gggcttgggc tccccctccg ccacggcggc
     1621 gccgtccagg tagccgacca gggaaggccc catggggacc accacgtcgc cgtcgagacg
     1681 accgaacccg ccggggaacg agaccagctt caacgcaccc acccctccat acgaccaggg
     1741 cagccttcaa cacgctgcgt gaccaaatgt agcgtgtcgt tgcttgctct ggtccggagc
     1801 ggccgctgcg gggtcgctac cgaggggtcc cggagcgggc agggaggccg ccggacaggg
     1861 tcgcggaggc gcgggcgagg gcgtgctgca ccagctcgat caaccccgcc ttggccgact
     1921 cccgcgagcg cgcgtcacac aacacgatcg gggtgtcctc ccccaccgcc aacgcatccc
     1981 gcacatcctc caccgcatgc gccaacaacc cattgaaaca gttgatcgcc acgatgaacg
     2041 gcagctcccg ctgctcgaag tagtccaccg ccgcgaaaca atccgccaac cggcgcgtat
     2101 ccaccaacac caccgccccg atcgcgcccc gcaccaggtc atcccacata aaccagaacc
     2161 gatcctgccc c
//
LOCUS       DASWDC010000036         9651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_258851, whole genome shotgun sequence.
ACCESSION   DASWDC010000036 DASWDC010000000
VERSION     DASWDC010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9651
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258851"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2295
                     /locus_tag="VF995_01030"
     CDS             <1..2295
                     /locus_tag="VF995_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342184.1"
                     /translation="EAIGTGAAGGSTAKHARARLTDTLGHERNALAAVEALMGLLGLS
                     EQPPPVEELPAAVRRLFRAMARRRPLAVVLDDLHLAEPPLLDLLEDVVDTVRDAPLVF
                     YCLARQELFDLKPGWGGGRPNATTILLEPLDQDSCATLIENMLGTANIDAQAKSRVPE
                     IAEGNPLFIEELIAMLIDDDLLMQSAGAWVAKTDLSAVPIPPTISALLAARLERLEGE
                     ERALLGLASVIGPVFSHDALRALAPAPLRSRVGAKLAALTRKELIRPDHSDPSGEDAF
                     GFRHALIRDAAYGALPQRERADLHERFADWLEGMAEEQDRLGDASDAIAYHLEQACRY
                     RAALGLDVPALIHRAVDRLAVAAQRATDRWDHPAAIGLLGRARALLPADDLTSLELVP
                     ALVANLGIQGDPEAARDLAASGIEQARMIRERRLEDRIRIEQMFVQQMVSGDTTLWSA
                     SAARREVELAIPLFEQEGDTQGLGAAWYLAGRIEWLSLRCTAMAAAVEQAIEYFRSGG
                     SHGRMLFALQLLSHAYWYGPVPVPEAIRRCEALLREAGDNRAVHAVIQGSIACMEAMR
                     GDSERAWRLLDEVAVVLVDVGQMVGWMPWAVYHEHSAFVARLERDYARMERSLRNILD
                     QRTLANPSVMLSSDAAWLAEAVDEQGRHEEAAKLCAAAERLATADDMLSQLFWRSVKA
                     RALARDGQLEPALRLTNAAVRLAAQTDAINWQANAWMDHAMTLWLARFDAEAEQAFQS
                     AVQRYQQKGNLVAEAQARALLAKG"
     gene            complement(2699..3025)
                     /locus_tag="VF995_01035"
     CDS             complement(2699..3025)
                     /locus_tag="VF995_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342185.1"
                     /translation="MRPRPLQAKHGNPHACKPVPQRGHRQPRRGEEGEQRLGPSARPR
                     LEPPGSFQDPRLYQTVKLATQVALIHGGDRAGNLGQASAAVEQRKDPPLEDGNRCSFD
                     PHKQRV"
     gene            2996..5092
                     /locus_tag="VF995_01040"
     CDS             2996..5092
                     /locus_tag="VF995_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HEX9342186.1"
                     /translation="MLGLEGTGAHAAVLLAACGVGELVLVDPYPCQAGNLPLMPVGAD
                     ALGMRRDQALKRSLETQGAETRLEIGPSDAVTPDGIHALARNCQLLIGAFDKGLSVAH
                     RWLNNASLTYGIPAIYSQLAAYVARVGPVVAPPRSACYECWRVRSLACADSYPEAAAY
                     QDLLDQQRQPRLHERPVFPALAAYAGSLVAMEAAKLLLSIASGADTLVGRVHEFDALQ
                     LRSQTHTLLQFPNCPECRGEDHTQPSFINLFHDNAPSGHIRQAAGSLVSPRCGVITDV
                     GQVPKDPDEPAQPYLYGARLANHHFVADRGPDDGISIGKGLTPAAAQSSALGEAVERY
                     AGSFHGVRQSFHASRQALDAPSLDPADLVLYRPEQYARLPYAPYTDTATLGWVAARSL
                     LSGSEMLVPALAVFIAYPPRAPEEDLCPPTSTGLAAGATLAEAVLAALYEVLERDAFM
                     ITWLNRLPAERVDPATHPDPELVDLVHAYARRGVALRLYRLATDHPCQVFLCLALQDG
                     GAGWPAAVAGLGADLDPARAASKAILEAGQVRAGLVVAARDPGERARAGRLVADPRLV
                     DTLDDHALRYIHPGAIGAFAFLERDPEPPAASAPGWSWAAPLPASASDRLRLLAEHLH
                     TSGSEVLYRDLTTADLAPLGLRVARVIVPGFQPLHFGQEPRLGGRRLYELPWRLGLAA
                     APATPAALNRDPHPLA"
     gene            5154..5558
                     /locus_tag="VF995_01045"
     CDS             5154..5558
                     /locus_tag="VF995_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342187.1"
                     /translation="MKNLPGTWDANLKLVYAVDQGAFFAIDTVGVGKAFDVIANLEIG
                     DDLMAVASKYDVYVAVRNLSQSTTVLSKHDTVILTPAHTDFNQEHRVSFPTSWTGGND
                     GDVLEVVAGFKVTAGVHTDYSLSTSNPFMVAA"
     gene            5689..8766
                     /locus_tag="VF995_01050"
     CDS             5689..8766
                     /locus_tag="VF995_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9342188.1"
                     /translation="MATATSQSFVGRAEELGRLLGVLARAEEGRPAVALVAGDAGVGK
                     TRLLAELANHAEAQGVRVLVGGCMEVGDLGLPYVPFVDAFRDLGARPGEAELAGELAA
                     TVPGLGLLLPRRGAASGTGGDRRSPAPAPESAPALAQPPGEGFERVRLFDGVLSLLVG
                     LAERAPLLLVIEDLHWADRSTRDLVAFLIRTLRTGRVALVASYRSDELHRRHPLRPLL
                     AELVRVPDLERVELRPFSRGELAQYLAGLEGADVPPDAVDRILARSEGNPFFAEELVA
                     AGATRRDVALPEALADVLRDRVEALSDLAQQVLKVAAVAGRRVSHRLLLAAAGLPEAE
                     LEQGLREAIAAQVLVADPATESYRFRHALLQEVVYGDLLPGERTRLHATYARLLAGKA
                     AAGTGDPGQDPDWDRDGGGAAELAYHALASHDLPGALAALLCATADATAMSAPAEAAR
                     HLEQALELWDQVPAAVAVAGTDRVELLLRAAEAANLSGEFRHAIKLTREAIAAIDPAT
                     DPLRAAAASDRLSEFLLDYHGDDEAHGEMLAASRRAVELVDAEPPTALRARVTGQLGR
                     TLTHLHQHDEARHWTDEALAVARKVGSATDEARALLVLALLAVRHNDIERARSLLEEA
                     RRRAAEAGDRMVELRAYYYQGNLEYDVGNLAAASAAFGQAVAHAERSGLRWSGVGADA
                     GVLRCIALYTAGDWDQAERLSVTVDDRSPAAGGLSAAALLVEVGRGSPSAQQRFARLR
                     AFFDDRWVAYLAASAGADLALWDGDLDQARALIRFCLSSVAVSEDPWALSAIRPAALG
                     ITAEAERAAQARIAGDEAAIAEATKVGKALLERARAAERQARSLSLQVGPEATAWLAR
                     AEAEWTRLQGHADPERWAAAAGAFAYGYAYEEARARWRLAEALLAGGHREEAKAELRA
                     AHQAADRLGAAPLRAELGALARRGRLDLGLGGGDLHDDPAAGVLGGLTPRELEVLRLV
                     AAGRSNQQIADTLYISRKTASVHVSNILGKLGVHTRVEAAATAHRLGLDGLKA"
     gene            complement(8858..8986)
                     /locus_tag="VF995_01055"
     CDS             complement(8858..8986)
                     /locus_tag="VF995_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342189.1"
                     /translation="MAAILLMIGLVVLGVFGGLFGTDTRDSSDWSRSTTDLHSHNR"
     gene            complement(8988..9131)
                     /locus_tag="VF995_01060"
     CDS             complement(8988..9131)
                     /locus_tag="VF995_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342190.1"
                     /translation="MATTLGDYSTIATKQRIAELVRAAEDDHRVRSARAARRRHSKLL
                     RRN"
     gene            9285..>9651
                     /locus_tag="VF995_01065"
     CDS             9285..>9651
                     /locus_tag="VF995_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9342191.1"
                     /translation="MRRIDLHAYPGTREWLDSQGPFAEALGRYWGRQWTAASEVEVVA
                     NFKEAGVEVVLVAFDIESVVGAPPCGNDYVAAFRDRHPGTILQAWGAVDPHKGDAAIR
                     EAERAVRELGVLGFHFHPIM"
BASE COUNT         1364 a         3523 c         3331 g         1433 t
ORIGIN      
        1 gaagcgatcg ggacgggcgc cgccggcgga agcacagcga agcacgcccg ggcgcgcctc
       61 accgacacgc tcggccacga gcgcaacgcc ttggcggcgg tcgaggcgct catgggcctg
      121 ctcgggctgt cggagcagcc gcccccggtg gaggagcttc ccgccgcggt gcgcaggctc
      181 ttccgggcga tggcccggcg gcgccccctg gcggtcgtcc tggacgacct gcacttggcc
      241 gagccgcccc tgctcgacct gctcgaagac gtcgtcgaca ccgtccgcga cgccccgctg
      301 gtgttctact gcctcgcccg ccaggagctg ttcgacctca aacccggctg gggcgggggc
      361 aggccgaacg ccaccacgat cctgctggag cccctcgacc aggattcctg cgccacgctg
      421 atcgagaaca tgctcggcac cgccaacatc gatgcgcagg ccaagtcccg cgtgcccgag
      481 atcgccgaag gcaacccgct gttcatcgaa gagctgatcg ccatgctcat cgacgacgac
      541 ctgctgatgc agtccgccgg agcatgggtg gccaagaccg acctcagcgc ggtcccgatc
      601 cccccgacga tctcggcgct gctggccgcc cggctggaac gcctggaagg cgaggagcgg
      661 gccctgctgg gcctggcgag cgtgatcggc ccggtcttct cccacgacgc cctgcgggcg
      721 ctggcgccgg cgccgctccg gtcgcgtgtc ggcgccaagc tcgcggccct cacccgcaag
      781 gagctgatcc gccccgacca ttcggatccc tccggtgagg acgccttcgg gttccgccac
      841 gcgctcatcc gcgacgccgc ctacggtgcc cttccccagc gggaacgcgc cgatctgcat
      901 gagcggttcg ccgattggct ggagggcatg gccgaggagc aggaccggct gggcgatgcc
      961 agcgacgcca tcgcctacca cctcgagcag gcctgccgct accgcgcggc attgggcctt
     1021 gacgtccccg ccctcatcca ccgggcggtt gaccgcctcg cggtggcggc acagcgggca
     1081 actgaccgtt gggatcaccc ggccgccatc ggcctgctgg gccgcgcccg ggcgttgctg
     1141 cccgccgacg acctgaccag cttggagctg gttcccgcgt tggttgccaa cctgggcatc
     1201 caaggtgatc cggaagccgc cagagacttg gccgcgagcg gaatcgagca ggcgcgcatg
     1261 attcgcgagc gtcggctgga agaccgaatc cgaatcgagc aaatgttcgt ccagcagatg
     1321 gtctcgggtg acaccacctt gtggtcggca tccgccgccc gccgggaggt ggagctggcg
     1381 attccattgt tcgagcagga aggggatacc caaggactcg gagccgcatg gtatctggcg
     1441 ggaagaattg aatggctctc gctgcgctgc accgcaatgg cagctgcggt cgagcaggcc
     1501 atcgaatact tccgctcagg gggaagccac ggacgaatgc tattcgcgct ccaactcctc
     1561 agccatgcct attggtatgg ccctgttccg gtccccgaag ccatccgccg ttgtgaagcc
     1621 cttcttcgtg aagcgggtga caaccgtgcg gtgcatgccg tgattcaggg cagcatcgcc
     1681 tgtatggagg ccatgcgcgg tgattccgaa cgcgcatggc ggctcctcga tgaggtagcc
     1741 gtcgtcctcg tggacgtcgg ccagatggtc gggtggatgc catgggctgt ctaccatgag
     1801 cacagtgcgt tcgtcgcgcg cctggagcgc gactatgcgc gcatggagag gtcgttgaga
     1861 aacatccttg accaacgcac gctggcaaat cccagcgtca tgctgtcgag cgacgctgcc
     1921 tggttggccg aggccgtcga cgagcagggc cgtcacgagg aggcggcgaa gttgtgcgct
     1981 gccgctgagc ggttggcgac cgcggatgac atgctctccc agcttttctg gcgaagtgtc
     2041 aaggcccggg cgctcgcccg cgatggccag ctcgagcctg cgttgcggct gacgaacgct
     2101 gcggtgcggc tcgcggccca gaccgatgcg atcaactggc aggccaacgc ctggatggac
     2161 cacgccatga ccttgtggtt ggccaggttt gacgccgagg ccgagcaggc cttccagtcc
     2221 gcagtccagc ggtatcagca gaaagggaac ctcgtagcgg aggcgcaagc tcgcgccttg
     2281 ctcgcgaaag gttgactgga ggtcttattg cgctcaatct tccagaaggg tcattagcga
     2341 cgcgaagggc tgggggagcg acatgcggca acggtttgag cgtgagacgc ttcagttgcg
     2401 tgtcgcgagg gtggaggacg aactcagaga gccaacgcga ccgccgtcgg ccttcagtga
     2461 cgacgacctc ctcgtgtacg tgagagttga gcgggctctc cactacgagt ttcatgtgat
     2521 cgcgcaagta tgcatcccgc cgcacaaacg cccctatcca cagatggatg agggtgaaag
     2581 cgctgaggag tccgcagaag agcccacgta gccgatgacc gaggagagcc ggctctttta
     2641 ccggccggcg gctgggatcg atgtcgtctc ggttggcgac ggcgcgctgt tgtttcgctc
     2701 agacacgctg cttgtgcgga tcgaaggagc atcggttgcc gtcctccagc ggcggatcct
     2761 tccgctgctc gacggcagcc gaagcttggc cgaggttgcc cgcgcgatcc ccaccgtgga
     2821 tcagggcgac ttgcgtcgcc agcttgacgg tctggtacag gcgggggtcc tggaacgaac
     2881 caggcggctc gagtcgtggc cgggcggaag gtccgagccg ctgctcgccc tcctcgccgc
     2941 ggcggggctg ccggtggccg cgttgcggga ccggcttgca cgcgtgcgga ttgccgtgct
     3001 tggcctggag gggacggggg cgcacgctgc ggtgcttctc gctgcttgtg gggtggggga
     3061 gctggtcctg gtcgacccat acccctgcca ggccggcaac ctaccgttga tgccagtcgg
     3121 cgcagacgcg ctgggaatgc gacgcgacca ggcgctcaag cgctcgctgg agacccaggg
     3181 cgctgagacg cggctggaga tcggaccgtc cgatgcggtc accccggacg ggatccatgc
     3241 gctggccagg aactgccagt tgctgatcgg cgcttttgac aagggcctgt ccgttgcaca
     3301 ccggtggctg aacaacgcca gcctgaccta cggaatcccc gcgatctaca gtcagttggc
     3361 cgcctacgtc gcacgagtgg gacccgtggt cgcgccgccg cggagcgcct gctacgaatg
     3421 ctggcgggtg cgcagcctcg cctgcgcgga ctcctacccg gaagcggcgg cctaccagga
     3481 cctgttggac cagcagcggc agccgcgcct gcacgagcgt ccggtgtttc cagcccttgc
     3541 cgcatatgcc gggagcctgg tcgccatgga ggcggccaag ctgctgctct cgatcgcttc
     3601 cggcgcggat accctcgtcg gaagggtgca cgagttcgat gcgctgcagt tgcggagcca
     3661 gacccatacc ctcctccagt tcccaaactg cccggaatgc cgtggggagg atcatacaca
     3721 accttccttc atcaacctat ttcatgacaa cgcaccttct ggacacattc gacaagcagc
     3781 cgggtcactc gtcagcccac gatgcggcgt gatcactgac gttgggcaag ttcccaagga
     3841 tcccgacgag ccggcgcagc cgtacctcta tggtgcccga ttggcgaatc atcacttcgt
     3901 cgcagatcgc ggccccgacg acggcatctc tatcggcaaa ggcctcaccc cagccgcggc
     3961 tcagtcgagc gccctcggcg aggcagtgga gcgctacgcc ggcagcttcc atggcgtccg
     4021 acaatctttc cacgcgtcac gccaggcgct cgacgcgccg tcgctcgacc cggcggacct
     4081 ggtcctctat cgccctgagc agtacgcccg gctgccgtac gctccctaca ccgacacggc
     4141 gacgctcggc tgggtggcgg cgcgctcgct gctcagcggg tcggagatgc tggtccccgc
     4201 cctcgcggtc ttcatcgcgt atccgccgcg cgctcccgaa gaagacctct gcccgcccac
     4261 ctcgactggc cttgccgccg gggcgaccct tgcggaggcg gtgctggccg ccctctatga
     4321 ggtgctcgaa cgcgacgcgt tcatgatcac ctggctgaac cgtctcccgg ccgagcgggt
     4381 ggaccccgcc acccatccgg accccgagct ggtcgacctg gtccacgcct acgcccggcg
     4441 cggcgtcgcg ctgcgcttgt accgcctggc gaccgaccat ccctgccagg tgttcctctg
     4501 cctcgcgctc caggatggcg gggcgggctg gccggcggcg gtcgccgggc tcggcgcgga
     4561 cctggacccg gcgcgggcgg cctccaaggc gatcctggag gcggggcagg tccgcgcagg
     4621 gttggtggtg gcggcgcgtg acccgggcga gcgggcgcgg gccgggcgac tggtcgccga
     4681 tccccggctg gtcgacaccc tcgacgacca cgcgctccgc tacatccacc ctggcgcgat
     4741 cggcgccttc gccttcctgg agcgcgaccc ggagccgccg gccgcctccg cccctggctg
     4801 gagctgggcc gcgccactgc cggcctccgc cagtgaccgc ctgcggctgc tcgccgagca
     4861 cctccacacc agcggcagcg aggtgctgta ccgcgacctc accaccgctg acctggcgcc
     4921 gctcgggctg cgggtggccc gggtaatcgt gcccgggttc cagccgctcc acttcgggca
     4981 ggagccgaga ctcggcggcc gccggctcta cgagctgccc tggcgactcg ggctcgccgc
     5041 cgcgcccgcc accccggcgg cgctcaaccg cgacccgcat ccgctcgcct gagagatttt
     5101 tgtatccagc ggcggcctcc ctccctcttc tcgcaccaga cgataggaag gaggtgaaga
     5161 atttgcctgg tacctgggat gccaatctca agctggtgta cgccgtcgat caaggagcat
     5221 ttttcgcgat cgatacggtt ggagtcggta aggcattcga cgtgatcgcg aacctggaaa
     5281 tcggggatga cctgatggcg gtagcgtcga agtacgacgt ctatgtggcg gtccgcaacc
     5341 tcagccagtc caccaccgtg ctcagcaagc acgacaccgt gatcctgaca cccgcacaca
     5401 ccgacttcaa ccaggagcat cgtgtcagtt ttccgaccag ctggaccggc gggaacgacg
     5461 gtgacgtcct cgaggtggtc gcggggttca aggtgacagc cggcgtccac acggactact
     5521 ccctgtcgac cagcaatccc tttatggtgg ccgcataacc acggcaaccg catcaccaag
     5581 gcctgccgca cgcatgcctg acctgatccc ggcgggtggc gcatcgccgc ccgccgggct
     5641 ttcgtaattt tggtgtcgtt gtcagggcgc accggcatac tggcaaccat ggcaaccgcg
     5701 accagccaat cctttgtcgg ccgtgccgag gagctcggac ggctgcttgg cgtgctggca
     5761 cgggccgagg agggccgccc ggcggtcgcg ctggtcgccg gggacgccgg cgtcggcaag
     5821 acccgcctgc tcgccgagct ggccaaccac gccgaggcgc agggcgtgcg ggtgctggtc
     5881 ggcggctgca tggaggtcgg cgacctcggc ctgccctacg tcccgttcgt ggacgccttc
     5941 cgcgacctcg gcgcccggcc gggtgaggcc gagctggcag gcgagctggc cgccaccgtc
     6001 cccggcctcg gcctgctgct gccccgcagg ggagccgcct cgggcaccgg cggcgaccgg
     6061 cggagccccg cccccgcacc ggagtcggcg ccggcgctgg cgcagccgcc aggagagggg
     6121 ttcgagcggg tccgtctctt cgacggggtg ctgtcgctcc tggtcggcct ggccgagcgg
     6181 gcgccgctgc tgctggtgat cgaggacctg cactgggccg accgctccac ccgcgacctg
     6241 gtcgccttcc tgatccgcac cctgcggacc ggccgcgtcg cgctggtcgc cagttaccgc
     6301 tccgacgagc tgcaccgccg ccacccgctg cgcccgctgc tggccgagct ggtccgcgtc
     6361 cccgacctgg agcgcgtcga gctgcgcccc ttcagccgcg gcgagctggc ccagtacctg
     6421 gcgggactgg aaggcgccga cgtccccccc gacgcggtcg accggatcct ggcccggtcc
     6481 gaaggcaacc ccttcttcgc cgaggagctg gtcgccgccg gtgccacccg ccgggacgtg
     6541 gccctgcccg aggcgctcgc cgacgtgctg cgcgaccgcg tcgaggcgct ctccgacctc
     6601 gcccagcagg tcctcaaggt cgccgcggtc gccgggcgcc gggtcagcca ccggctgctg
     6661 ctggccgccg ccggcctgcc cgaggccgag ctcgaacagg gcctgcgcga ggcgatcgcc
     6721 gcccaggtcc tggtcgccga ccccgccacc gagagctacc gcttccgcca cgcgctgctg
     6781 caggaggtcg tctacggcga cctgctgccg ggggagcgca cccgcctgca cgccacctac
     6841 gcgcgcctgc tcgccgggaa ggcggcggcc gggacgggcg atccgggcca ggacccggac
     6901 tgggaccggg acggcggcgg cgccgccgag ctggcctacc acgcgctggc cagccacgac
     6961 ctgcccggcg cgctggccgc gctgctgtgc gccaccgccg acgccaccgc catgtccgcc
     7021 ccggccgagg cggcccgcca cctggagcag gccctggagc tgtgggacca ggtccccgcc
     7081 gccgtcgccg tcgccgggac cgaccgggtc gagctgctgc tgcgcgccgc cgaggccgcc
     7141 aacctctccg gcgagttccg ccacgccatc aagctcaccc gcgaggcgat cgccgccatc
     7201 gaccccgcca ccgacccgct gcgggcggcg gcggcctcag accgcctcag cgagttcctg
     7261 cttgactacc acggcgacga cgaggcgcac ggcgagatgc tggccgcctc gcgccgcgcc
     7321 gtcgagctgg tcgacgccga gccgccgacg gcgctgcggg cgcgcgtcac cgggcagctc
     7381 ggccgcaccc tgacccacct gcaccagcac gacgaggcac gccactggac cgacgaggcg
     7441 ctggcggtcg cccgcaaggt cggcagcgcc accgacgagg cccgcgccct gctggtcctc
     7501 gccctgctgg cggtccgcca caacgacatc gagcgggcac gctcgctgct ggaggaggcc
     7561 cgccgccgcg ccgccgaggc cggcgaccgg atggtggagc tgcgcgccta ctactaccag
     7621 ggcaacctcg aatacgacgt cggcaacctg gccgccgcct ccgccgcctt cggccaggcc
     7681 gtcgcccacg ccgagcgcag cgggctgcgc tggagcggcg tcggcgccga cgcgggcgtg
     7741 ctgcgctgca tcgccctgta caccgccggc gactgggacc aggccgagcg cctctcggtc
     7801 acggtcgacg accgcagccc cgcggccggc ggcctctccg ccgccgccct gctggtcgag
     7861 gtcggccgcg gcagccccag cgcccagcag cgcttcgcgc ggctgcgcgc cttcttcgac
     7921 gaccgctggg tcgcctacct cgccgccagc gccggtgccg acctcgcgct ctgggacggc
     7981 gacctggacc aggcccgcgc gctgatccgc ttctgcctgt cgtcggtggc ggtgagcgag
     8041 gacccgtggg cgctgagcgc gatccgcccg gcggcgctcg gcatcaccgc cgaagccgag
     8101 cgggccgcgc aggcccgcat cgccggcgac gaggccgcca tcgccgaggc caccaaggtg
     8161 gggaaggcgc tgctggaacg ggcccgcgcc gccgaacggc aggcccgctc cctcagcctc
     8221 caggtcggcc cggaggcgac tgcctggctc gcccgcgccg aggccgaatg gacccgcctg
     8281 cagggccacg ccgacccgga gcgctgggca gcggccgccg gcgccttcgc ctacggctac
     8341 gcctacgagg aggcccgcgc ccgctggcgc ctcgccgagg cgctgctggc cggcggccac
     8401 cgggaggagg cgaaggccga gctgcgcgcc gcccaccagg cggctgaccg tctcggcgcc
     8461 gcgccgctgc gggccgagct cggcgcgctc gcccgccggg gccgcctcga cctcggcctg
     8521 ggcggcggcg acctccacga cgacccggcg gccggcgtgc tcggcggcct caccccccgc
     8581 gagctggagg tcctccgcct ggtggcggcc ggccgctcca accagcagat cgccgacacg
     8641 ctgtacatca gccgcaagac cgccagcgtc cacgtcagca acatcctcgg caagctcggc
     8701 gtccacaccc gggtcgaggc cgccgccacc gcccaccgcc tcggcctgga cggcctgaag
     8761 gcatagaacc tggcggaata gaaagaggac ggcgcgtgtg gtgggtgcgc gccgccctcg
     8821 ttggtgggcc ctgtggtcgg ctggaggggc cgagggccta ccggttgtga gagtgcaggt
     8881 ccgtcgttga ccgtgaccag tcgctgctgt cgcgggtgtc ggtgccgaac aggcccccga
     8941 agacccccag gaccaccagg ccgatcatga gcaggatagc tgccatctca gttcctcctc
     9001 agaagcttgc tgtggcgccg gcgggcggcg cgggcgctcc ggacccggtg gtcgtcctcg
     9061 gccgcgcgga ccagttcggc gatgcgctgc ttggtcgcga tggtgctgta gtcaccaagg
     9121 gtggttgcca tggtggtctc ctctcctgcc cgcttccttc tctgttctcg ctgtccatac
     9181 agtaggtcta aggcggccgg tcccacctcg ggctggcgcc ttagatccgc agctcaggcg
     9241 ccttatttgt gtaataccta ccttggtagg taacctggaa ggcaatgcgc cgcatcgacc
     9301 tgcacgccta cccgggaacc cgcgagtggc tcgactcgca gggccccttc gccgaggcgc
     9361 tcggcaggta ctggggacgc cagtggacgg ccgccagcga ggtcgaggtg gtcgccaact
     9421 tcaaggaggc cggcgtcgag gtggtgctgg tcgccttcga catcgagtcg gtggtcggcg
     9481 ccccgccgtg cggcaacgac tacgtggccg ccttccgcga ccggcaccct ggcacgatcc
     9541 tgcaggcctg gggcgccgtc gacccgcaca agggcgatgc ggccatccgg gaggccgagc
     9601 gggccgtgcg cgagctcggc gtgctcggct tccacttcca cccgatcatg g
//
LOCUS       DASWDC010000037         5570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1261000, whole genome shotgun sequence.
ACCESSION   DASWDC010000037 DASWDC010000000
VERSION     DASWDC010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261000"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VF995_01070"
     CDS             <1..249
                     /locus_tag="VF995_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9342192.1"
                     /translation="TLGVPTVVLTGGNDHTVPPTAAAALASGIPGARLEQLPGLGHLL
                     PQQAPEAVARAVQEVADAARVTDGPPDHATEPPVAAAE"
     gene            368..748
                     /gene="hisI"
                     /locus_tag="VF995_01075"
     CDS             368..748
                     /gene="hisI"
                     /locus_tag="VF995_01075"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX9342193.1"
                     /translation="MAALIAGLTFDARGLLPAVVQDHRDQTVLMVGWMDAEAVRRTLT
                     SGRTWFWSRSRQEYWCKGETSGHRQYVRSVHTDCDRDTLLVQVEQVGAACHTGTRSCF
                     AAELPTVTGEAPAGAHARADGAAR"
     gene            745..2412
                     /gene="trpE"
                     /locus_tag="VF995_01080"
     CDS             745..2412
                     /gene="trpE"
                     /locus_tag="VF995_01080"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010306769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX9342194.1"
                     /translation="MTGQPSSGGLGSLPALDEFAKLAAEYSIVPVWRELLGDLETPVG
                     AFRKLTATDDPDATAGSVLLESVEHGERWGRYSFIGTDPFATFTAHRGSVSWSGSPPA
                     GLPATGADGSPLRVLRAALERLRAPVIGGLPPLYAGAVGYLAYDAVRELERLPDQTSD
                     DLGLPDGILVFPRSVVVFDHLGQRLVLVTNVLTDEPGFDPERAYAAATARLDELAERL
                     SVATPTPPTPLPAAGPPAAAESDLAPGRYQEMVEIAKEHIRAGDIFQVVPSQRFSVKT
                     SADPFDVYRVLRVVNPSPYMYFLRLPGGSGGLKSEAPTPTSGDPGKPSGRPPVNKTVT
                     ISGSSPEVLVTVQGREAVMRPIAGTRPRGADPAADLALEEELKADEKERAEHVMLVDL
                     ARNDLGRVCRPGTVKVRDFMRVERYSHVMHLVSDVVGELADHVTAFDVLASSFPAGTV
                     SGSPKVRAMEIIDDLERHRRGPYAGCVGYFDFSGNLDTCITIRTCVFIGDTAYCQAGA
                     GVVADSVPEREERETEAKARALLAAVAAAEALPRATLMEAAGGRPDG"
     gene            2405..3079
                     /locus_tag="VF995_01085"
     CDS             2405..3079
                     /locus_tag="VF995_01085"
                     /inference="COORDINATES: protein motif:HMM:NF021079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HEX9342195.1"
                     /translation="MAEPPAPASAGRRSLQASLLSVLAGVLLAVLGAGRPWVSVSGQP
                     LVANLNGPTVVTASLSGNDLAPLSTLALLGLVLVVAVAMTRGRGRWLVGLALLAIATV
                     LVAQAVGAAGRAHSEAEARFRRGALPGLPASFAHHTSSSATGPALVVAGGLLVGLAGV
                     EALRRGPGWPALGEAFRAPPDRDLDRDLDRSRDRRFDGSLDSDMGRARTPSGDPDEEP
                     PWEEAP"
     gene            3213..3914
                     /locus_tag="VF995_01090"
     CDS             3213..3914
                     /locus_tag="VF995_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9342196.1"
                     /translation="MEQAGKGTILLIEDEESIAEVVRLYLERDGFRVVWRTDGRAGLA
                     AVDSERPRLVLLDLMLPGLDGLEVTRQLRGRGGARVPIIMVTAREEEADRVLGLELGA
                     DDYVTKPFSPRELVARVKAVLRRAEPEPDEAPEPPLHLGTLTVDPARHEVSFEGRQVD
                     LTAREFELLSYLVRNRGLVLSRDQILERVWGYAFPADTRTVDVHIRQLRAKLGDHAPI
                     RTIRGVGYKADDRAE"
     gene            3911..5470
                     /locus_tag="VF995_01095"
     CDS             3911..5470
                     /locus_tag="VF995_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9342197.1"
                     /translation="MNLHPQPAPPARSGSLAGRGSLWGQLVWGFVAVAALAALITGAI
                     ALSLIRLVPDRSLEQQLSTEADAIVSSQLTAQNILGSCLVQRVSQVSGTAMYFINREG
                     TPTLTCPSRTFARVTNAAPPPDVDLRGALTTSGGQITSGTKGGLVYAVAPLSGAGANR
                     AGVLLVHPARGLARSLVGPLAWRLLFATALAVVIAALVAAALAGRLTAPLGRLVGAAR
                     NLGAGDLSTRVAVDGDDEVAEVGAAFNEMAAALEQSQGEQRAFLASVGHELKTPLTAV
                     QGYTEALLDGTVEDPDARRRSLERIHAETLRLARLVQDLLDLARLGRGQFAVSLVDTD
                     AATVLREAAASWSERAAARGVTVEQRLPAQLPARVDPGRLRQVLDNLLDNATRSSPAG
                     HPVLLAAVQLPSGGAEVAVVDQGPGIAAEDLAHVFDRGYLWSRYRGTRAVGSGLGLAI
                     VKALCDAMRVTIWAESGGGGGTAFRLTLPPAASSAGFGPMPTQELPVPSPTVSARGAG
                     KGTAAPPWESG"
BASE COUNT          715 a         2204 c         1899 g          752 t
ORIGIN      
        1 accctcggcg tccccaccgt ggtgctgacc ggcggcaacg accataccgt cccaccgacc
       61 gccgcggccg cgctggccag cggcatcccc ggcgcccggc tggagcagct gcccggcctc
      121 ggtcacctgc tgccccagca ggccccggag gcggtcgccc gcgccgtcca ggaggtcgct
      181 gacgccgccc gcgtcaccga cgggccaccg gaccacgcca cggagccccc tgtcgccgcc
      241 gcggagtagc gtctcgaatt gcatcccggc atagcatgag tgaccgaccc gatccggcca
      301 cgcccaagga gcgtccgtgt ccgaccccgc ccccacccga tccgctgccg atcctgccag
      361 cgacgacctg gccgcgctga tcgccggcct caccttcgac gcgcgggggc tgctccccgc
      421 cgtggtccag gaccacaggg accagactgt gctgatggtc ggctggatgg acgccgaagc
      481 cgtccgccgc accctgacca gcggccggac ctggttctgg agccgttccc ggcaggagta
      541 ctggtgcaag ggcgagacct ccgggcaccg gcagtacgtc cgctctgtcc acaccgactg
      601 cgaccgcgac accctcctgg tccaggtcga gcaggtcggc gccgcctgcc acaccggcac
      661 gcgcagctgc ttcgccgccg agctgcccac ggtgaccggc gaggccccgg ccggcgcgca
      721 cgcccgagct gacggggcgg ccaggtgacc ggccagccca gctctggcgg cctcggctcc
      781 ctgccggccc tggacgagtt cgccaaactg gcagccgagt actccatcgt gccggtgtgg
      841 cgtgagctgc tcggcgacct ggaaaccccg gtcggggctt tccgcaagct gaccgcgacc
      901 gacgacccgg acgcgactgc tggatcagtg ctgctggaaa gcgtcgagca cggggagcgc
      961 tggggccgct actccttcat cggcaccgac ccgttcgcga ccttcaccgc ccaccgcggc
     1021 agcgtctcct ggagcggcag cccacccgcc gggctgcccg ccacgggtgc tgacggctcg
     1081 ccactgcggg tcctgcgcgc cgcgctggag cggctgcgcg ccccggtcat cggtgggctg
     1141 ccgccgctgt atgccggcgc ggtcggctac ctcgcctacg acgccgtccg cgagctggag
     1201 cgcctgcccg accagaccag cgacgacctc ggcctccctg acgggatcct ggtcttccct
     1261 cgttcggtgg tggtcttcga ccacctcggc cagcgcctgg tgctggtcac caacgtgctg
     1321 accgacgagc ccggcttcga cccggaacgg gcctacgccg ccgccaccgc ccggctcgac
     1381 gagctggccg agcggctcag cgtggccacc ccgaccccgc cgacgccgct ccccgccgcc
     1441 ggcccgcccg ccgccgccga gagcgacctc gcccccggcc gctaccagga gatggtggag
     1501 atcgccaagg agcacatccg cgccggcgac atcttccagg tggtcccgtc gcagcgcttc
     1561 tcggtcaaga ccagcgccga cccgttcgac gtctaccggg tgctccgggt cgtcaacccg
     1621 tctccctaca tgtacttttt gcgccttccc ggggggtctg gggggctcaa gagcgaggcc
     1681 ccgaccccga catccggtga cccaggtaag ccgagcggta gacccccggt caacaagacc
     1741 gtgaccatct ccgggtcctc gccggaggta ctggtcaccg tgcagggccg cgaggcggtc
     1801 atgcggccga tcgccgggac ccgcccgcgc ggcgccgacc ccgccgccga cctcgccctg
     1861 gaggaggagc tcaaggccga cgagaaggaa cgcgccgagc acgtcatgct ggtcgacctc
     1921 gcccgcaacg acctcggccg ggtctgccgg ccgggcaccg tgaaggtgcg ggacttcatg
     1981 cgggtggagc gctactccca cgtcatgcac ctggtctccg acgtggtcgg cgaactggcc
     2041 gaccacgtca ccgccttcga cgtgctggcc tcctcgttcc cggcgggcac cgtgtccggc
     2101 tcgcccaagg tccgcgccat ggagatcatc gacgacctgg agcgccaccg ccgcggcccc
     2161 tacgccggct gcgtcggcta cttcgacttc agcggcaacc tggacacctg catcaccatc
     2221 cggacctgcg tgttcatcgg cgacaccgcc tactgccagg ccggcgccgg cgtggtcgcc
     2281 gactcggtcc ccgaacgcga ggaacgcgag accgaagcca aggcccgcgc cctgctcgcc
     2341 gccgtcgccg ccgccgaggc cctgcccagg gcgaccttga tggaggcggc gggggggcgg
     2401 ccggatggct gagccgcccg ccccggcctc ggccgggcgc cgctccctgc aggcgagcct
     2461 gctgtcggtg ctcgccgggg tgctgctggc ggtgctcggc gcgggccggc cgtgggtcag
     2521 cgtcagcggc cagcccctgg tcgccaacct caatggcccg acggtcgtga ccgcgagcct
     2581 cagcggcaac gacctcgcac cgctgtccac gctcgccctc ctcgggttgg tcctggtggt
     2641 cgcggtcgcg atgacccgcg ggcggggtcg ctggctcgtc gggctcgccc tgctcgccat
     2701 cgcgaccgtc ctggtcgcgc aggcggtcgg cgccgccggg cgggcccatt cggaggccga
     2761 ggcgcggttc cgccgtggcg ccctgcccgg actgccggcc tccttcgccc accacaccag
     2821 cagctcggcc accgggccgg ccctggtggt cgccggcggc ctcctggttg gcctggccgg
     2881 agtcgaagcg ctgcgtcggg gccctgggtg gcccgccctc ggcgaggcct tccgcgcccc
     2941 gcccgaccga gacctcgacc gagacctcga ccggagccgc gaccggcgct tcgacgggag
     3001 cctcgactct gacatgggtc gtgcccgcac cccaagcggc gatcccgacg aggaaccgcc
     3061 ctgggaagaa gccccgtagg gttgccctga aagcgccctt gacctgcgag ttcacgttcc
     3121 tcgaacatgg cgtggctgca cgccgaacct gactgcggga tccttgtggg ggaggattcc
     3181 aggacgagcg cgggcgacca gcgggggcga aggtggagca ggcaggcaaa ggtaccatcc
     3241 tgctgatcga agacgaggag tcgatcgccg aggtcgtgcg cctctacctg gaacgcgacg
     3301 gcttccgggt cgtctggcgc accgacgggc gcgccggact ggccgccgtc gacagcgagc
     3361 ggccccggct ggtcctgctc gacctgatgc tgcccggcct cgacgggctg gaggtcaccc
     3421 ggcagctgcg cggccgcggc ggcgcgcggg tcccgatcat catggtgacc gcccgggagg
     3481 aggaggccga ccgggtcctc ggcctggagc tcggcgccga cgactacgtg accaagccgt
     3541 tctcgccccg ggagctggtc gcccgggtca aggcggtgct gcgccgcgcc gaacccgagc
     3601 ccgacgaggc gcccgagccg ccgctgcacc tcggaaccct caccgtggac cccgcccgtc
     3661 acgaggtcag cttcgagggc cgccaggtcg acctgaccgc ccgcgagttc gagctgctca
     3721 gctacctggt ccggaaccgt ggcctggtgc tctcccgcga ccagatcctg gagcgagtct
     3781 ggggctacgc cttccccgcc gacacccgca ccgtcgacgt ccatatccgc cagctccgcg
     3841 ccaagctcgg cgaccatgcc cccatccgta ccatccgggg tgtcggctac aaagccgacg
     3901 atcgcgcaga atgaatctcc acccgcagcc cgcaccgccc gctcgctccg gcagcctcgc
     3961 tggccgcggg tcgctgtggg gtcagctcgt ctggggattc gtggccgtgg cggcgctggc
     4021 cgcgctgatc accggcgcga tcgccctctc gctcatccgc ctggtccctg accgcagtct
     4081 ggagcagcag ctcagcaccg aggccgacgc catcgtgtcg agccagctca ccgcccagaa
     4141 catcttgggg agctgcctcg tgcaacgtgt gtcccaggtc agcggcactg cgatgtactt
     4201 catcaaccgc gaaggcaccc cgacgctcac ctgcccgagc cggaccttcg cgagggtgac
     4261 caacgcggcg cccccgcctg acgtggatct gcgcggcgcc ctcaccacaa gcggcggcca
     4321 gatcaccagc ggcaccaagg gcggcctggt ctacgcggtc gcgcccctgt ccggcgctgg
     4381 cgccaaccgc gcgggagtgc tgctggtcca cccagcccgc gggctcgccc gtagcctggt
     4441 ggggccgctc gcctggcggc tgctgttcgc caccgccttg gccgtggtga tcgcggcgct
     4501 ggtcgccgcc gcgctcgctg ggcggctcac cgcgccgctg gggcggctgg tcggcgccgc
     4561 gcgcaacctc ggcgccgggg acctctccac ccgcgtcgcg gtcgacggcg acgacgaggt
     4621 cgccgaggtt ggcgccgcct tcaacgagat ggccgccgcg ctggagcaga gccagggcga
     4681 gcagcgcgcc ttcctcgcca gcgtcggcca cgagctgaaa acccccctca ccgctgtcca
     4741 gggctacacc gaggcgctgc tggacgggac cgtcgaagac cctgacgcgc ggcgccgcag
     4801 cctggaacgt atccacgccg agacgctgcg cctcgcccgg ctggtccagg acctcctcga
     4861 cctggcccgg ctcggccgcg gccagttcgc cgtcagcctg gtcgacaccg acgccgccac
     4921 ggtgctgcgc gaggcggcgg cctcctggag cgagcgtgcc gccgcccgcg gcgtcaccgt
     4981 cgagcagcgc ctgcccgccc aactccccgc ccgcgtcgac cctgggcgcc tgcgccaggt
     5041 ccttgacaac ctgctcgaca acgccacccg ctccagcccc gccggccatc cggtgctgct
     5101 cgccgccgtc cagctcccca gcggcggcgc cgaggtcgcc gtggtcgacc agggcccagg
     5161 catcgccgcc gaggacttgg cgcatgtctt cgaccgcggc tacctgtggt ctcgctatcg
     5221 cggcacccgc gcggtcggct ctggcctcgg cctggcgatc gtcaaggcgc tctgcgacgc
     5281 catgcgggtg acgatctggg cggaatcggg cggcggaggt ggtaccgcct tccgcctcac
     5341 ccttccccca gccgcctcca gcgctggctt cggccccatg ccgacccagg agctgcccgt
     5401 cccgtcgccc accgtcagtg cgcggggggc cgggaagggg acggcggcgc ctccctggga
     5461 atccggttga gtcgctgacc ggcggcgccg gtagaatcgg ccgatgccag atcccgcgct
     5521 tggcgcgccc tcctcgttcc tcgaccgtgc ggtcgccgat gcccgcgagg
//
LOCUS       DASWDC010000038         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_676584, whole genome shotgun sequence.
ACCESSION   DASWDC010000038 DASWDC010000000
VERSION     DASWDC010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676584"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VF995_01100"
     CDS             <1..1459
                     /locus_tag="VF995_01100"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEX9342198.1"
                     /translation="VLADGTPHRRVPIARLIALLDRLAGEIPALLPRPSPPAGADLAP
                     VYQLASGGTVTFEPGGQVEHSTAPHRTAHAALADADGLAAALAAALGEAGVVLVSCGF
                     DPWHPPGRVPQQLRAPRYPAMAAYFDRRGPDGAAMMRCSAALQVNLDLGTTEDERRER
                     WLVANLVAPLALATFAASPDAERGVVSRRAHAWQRLDPTRTGFPRRLVDGSSADPVEQ
                     LLDTALHADVALFCRQSPGPVDNPGAGAAGIVPLELGMSAQRPVDSAVDRLVDNANSV
                     DGAPVDNADGWTDGWTDGWTDGWGPGRPGWTFGDWIRDGHPAHGPPTADDLRYHLSTL
                     FPEVRPRGHLELRSVDALPARLRPVPVVLLAGLLEDPGARAHGRAVLERWRPRLPELW
                     RRAAHAGVADPELCALAAEVWSFALEGARRLPPGYLTAAQVTLAECFLDRLTLRGRCP
                     ADELRAALARGPAAALAWASEPSAVAAPDPAESIR"
     gene            1537..2919
                     /gene="egtB"
                     /locus_tag="VF995_01105"
     CDS             1537..2919
                     /gene="egtB"
                     /locus_tag="VF995_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX9342199.1"
                     /translation="MPAATSNTPSDAALRRQLEDDLDTVRGRLGLLVAPLDDDAIHRQ
                     HDPIMSPLVWDMGHVGNFEELWLLRELDGRGAHDPTLDQVYNPFENPRWVRADLPILQ
                     RPEAVAYLADVRAEALEVLRRADLRDPGRPLLADGYVYRMITQHEAQHQETMLQTLDL
                     RADPDPYPPVAARRLLVPRPVDDRERVQISGGEFQMGTDDRGAAYDNERPRHPVTVPS
                     FWIDRFPVTARRFASFVAAGGYDQPEHWSAEGRAWLRETGDRAPQGWTPDGSGWKVRR
                     FGHLLPLDPREPVQHVSFHEAEAFAHWAGGRLPTEAEWEKAAAWDPAAGRSRTWPWGE
                     TPPSPQHANVDQLAFGPAPVGSYPAGASAYGVEQLLGDVYEWTSSPFTGYPGYATFPY
                     PEYSEVFFGDQYRVLRGASWATASWVARNTFRNWDYPKRRQLFAGLRLAWDAPPGQGL
                     DGGRNGGGPG"
     gene            2919..3764
                     /locus_tag="VF995_01110"
     CDS             2919..3764
                     /locus_tag="VF995_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342200.1"
                     /translation="MCRLAACLGPPAPLSVLLYDPPWSLNEQAYRPRRQEYGTVNVDG
                     TAVVWWPPGEAEPLRYATGGPPWADPNLPNLAPRLRGTVQLAAVRSATPGLPFGADAA
                     PPFMRGGLALAHNGRIEGFRGALGRGLLGRLPDDLHAALGPLPLHDSVALLLLTAWKR
                     RERPAAGLAGALGAAVTEVVAACAAAGERATLTAVAAEHGHLAGVRMAVGLAAPALFL
                     HIPAGPSGPEAETALIASEPLDDRPGWTEIGDRHLLDLSAAGATLTPLDAGMAAGADM
                     EKKSA"
     gene            3761..>4179
                     /locus_tag="VF995_01115"
     CDS             3761..>4179
                     /locus_tag="VF995_01115"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX9342201.1"
                     /translation="MTTRTLPSVTSLAPPADWREQLAADVRAGLTATPRTLPSKYFYD
                     AEGSELFERITRLPEYYLTRAEQEILAEHADRLLAAAQAEEVLELGSGSSRKTRLLLE
                     AMRRHNGGRRYAPLDVSEDALIGAALALCNDYPWLRF"
BASE COUNT          525 a         1679 c         1453 g          522 t
ORIGIN      
        1 agtcctggcg gacggcaccc cgcaccgacg cgttcccatc gcccggctga tcgccctgct
       61 cgaccggctc gccggcgaga tccccgccct gctcccccgc cccagccctc cggccggcgc
      121 tgacctggcg ccggtctacc agctggcctc cggcggcacc gtcaccttcg agcccggcgg
      181 ccaggtcgag cactccaccg caccgcacag gaccgcccat gcggcgctcg ccgacgccga
      241 cggcctcgcc gccgcgctgg ccgccgcgct cggcgaagct ggcgtcgtcc tggtgagctg
      301 cggcttcgac ccgtggcacc cacccgggcg ggtcccgcag cagctccgcg cgccgcgcta
      361 cccggcgatg gccgcctact tcgaccgccg cggtcccgac ggcgccgcga tgatgcgctg
      421 cagcgccgcc ctccaggtca acctggacct cggcaccacg gaggacgaac gccgggagcg
      481 ctggctggtc gccaacctgg tcgccccgct cgcgctcgcc accttcgccg cgagccccga
      541 tgccgaacgc ggggtggtgt cgcggcgcgc ccacgcctgg caacggctcg acccgacccg
      601 taccgggttc ccgcgccgcc tggtggatgg ttccagcgcc gatccggtgg agcagctgct
      661 cgacaccgct ctccacgccg acgtcgcgct gttctgccgc caatccccag gccctgtgga
      721 taaccctgga gccggcgccg ccggaattgt tccgcttgag ctggggatga gcgctcagag
      781 gccggtcgat tcggctgtgg atcgacttgt ggataatgcc aattctgtgg atggcgcccc
      841 tgtggacaac gccgacggct ggaccgacgg ctggaccgat ggctggaccg acggctgggg
      901 ccccgggcgg cccgggtgga cgtttgggga ctggatccgg gacgggcatc ccgcacacgg
      961 gcccccgacc gccgacgacc tgcgctacca cctcagcacg ctgttccctg aggtgcgccc
     1021 gcgggggcac ctggagctgc gctcggtcga cgcgctgccg gcccggctgc ggccggtccc
     1081 ggtggtgctg ctggccggcc tgctcgagga tcccggcgcc cgcgcccacg gccgtgccgt
     1141 gctggagcgc tggcggccac ggctgccgga gctgtggcgg cgcgccgccc acgccggggt
     1201 cgccgacccg gagctgtgcg cgctcgcggc cgaggtctgg tcgttcgcgc tggagggcgc
     1261 ccgccgcctg cccccgggat atctcacggc ggcgcaggtg acgctcgcgg agtgcttcct
     1321 cgaccggctg accctgcggg gtcgctgccc ggccgacgag ctgcgcgccg cactcgcccg
     1381 cggacccgcc gccgcgctgg catgggcgag cgaacccagt gcggtcgccg ccccagaccc
     1441 agccgagtcg atccgctgaa ccccacttcc acaagcagga accgacccgg ccgtggccac
     1501 ccgccgaggc cgaaccggaa gcgaggtccc accgtcatgc cagcagcaac gtccaatacc
     1561 ccatccgatg cggcgctgcg ccgccagctc gaagacgacc tcgacaccgt gcgcggtcgc
     1621 ctcggcctgc tggtcgcgcc gcttgacgat gacgccatcc accgccagca cgacccgatc
     1681 atgagcccgc tggtgtggga catgggccac gtcggcaact tcgaggagct gtggctgctg
     1741 cgcgagctgg acggccgcgg cgcccacgac cccaccctcg accaggtcta caaccccttc
     1801 gagaaccccc gctgggtccg cgcggacctg ccgatcctgc agcggcccga agcggtcgcc
     1861 tacctcgccg acgtccgcgc cgaggcgctg gaggtcctgc gccgcgccga cctgcgcgat
     1921 ccgggccggc cgctgctcgc cgacggctac gtctaccgga tgatcaccca gcacgaggca
     1981 cagcaccagg agaccatgct gcagaccctc gacctgcgcg ccgaccccga cccctacccg
     2041 ccagtcgcgg cccgccgcct gttggtcccc cgcccggtcg acgaccgcga acgggtccag
     2101 atctccggcg gcgagttcca gatgggcacc gacgaccgcg gcgccgccta cgacaacgag
     2161 cgtccccgcc acccggtgac cgtgccgagc ttctggatcg accgcttccc ggtgaccgcg
     2221 cggcgtttcg ccagcttcgt cgccgccggc ggctacgacc agcccgagca ctggagcgcc
     2281 gagggccggg cgtggctgcg ggagaccggc gaccgcgccc cgcagggctg gacgcccgac
     2341 ggcagcggct ggaaggtgcg gcgcttcggc cacctgctgc cgctggaccc gcgcgagccg
     2401 gtgcagcacg tcagcttcca cgaggccgag gcgttcgccc actgggccgg cggccggctg
     2461 cccaccgagg ccgaatggga gaaggccgcc gcctgggacc cggccgccgg gcgctcccgc
     2521 acctggccgt ggggcgagac cccgccctcc ccccagcacg ccaacgtcga ccagctcgcc
     2581 ttcggcccgg ccccggtggg cagctacccg gcgggcgcct ccgcctacgg ggtcgagcag
     2641 ctcctcggcg acgtctacga gtggacatcg tccccgttca ccggctaccc gggctacgcg
     2701 accttcccct accccgagta cagcgaggtg ttcttcggcg accagtaccg ggtcctgcgg
     2761 ggcgcgtcct gggcgaccgc gagctgggtg gcgcgcaaca ccttccgcaa ctgggactac
     2821 cccaagcgcc ggcagctctt cgccggcctg cggctcgcct gggacgcccc tcccggccaa
     2881 ggcctggatg gtggccggaa tggtggaggg ccgggctgat gtgccggctc gccgcctgtc
     2941 tcggcccacc cgcgccgctg tcggtgctgc tctacgaccc gccgtggtcg ctgaacgagc
     3001 aggcctaccg gccccgccgc caggagtacg gcaccgtcaa cgtggacggc accgccgtcg
     3061 tctggtggcc gcccggcgag gcggagccgc tccgctacgc caccggaggg ccgccctggg
     3121 ccgacccgaa cctgcccaac ctcgccccgc ggctgcgcgg gaccgtgcag ctcgcggcgg
     3181 tccgctcggc aaccccgggc ctgccgttcg gcgccgacgc cgcgccacca ttcatgcgtg
     3241 gaggcctggc gctggcgcac aacggccgca tcgaggggtt ccgtggggcg ctcggccggg
     3301 ggctgctcgg ccggctaccc gacgacctcc acgcggcgct cgggccgctg ccgctgcacg
     3361 actcggtggc gttgctgctg ctgaccgcct ggaagcgccg cgaacggccg gcggcgggac
     3421 tggcgggtgc gctcggcgcc gccgtcaccg aggtcgtcgc ggcgtgcgcg gcggcgggcg
     3481 agcgggcgac cctcaccgcc gttgccgccg agcacggcca cctggccggg gtgcgcatgg
     3541 cggtggggct cgccgcgccc gcgctgttcc tgcacatccc ggcgggaccg tcgggacccg
     3601 aggccgagac ggccctcatc gcctccgaac cgctcgacga ccgccccggc tggaccgaga
     3661 tcggcgaccg gcacctgctg gacctgagcg ccgccggggc gacgctgacg ccgctggacg
     3721 ccggcatggc cgccggcgcg gacatggaga agaagagcgc atgacgaccc gtaccctccc
     3781 atcggtcacc agcctcgcac cgcccgccga ctggcgggag cagctcgccg ccgacgtccg
     3841 cgcgggcctg accgcaacgc cgcggacgct tccgtccaag tacttctacg acgccgaggg
     3901 ctccgagctg ttcgagcgga tcacccgcct gcccgagtac tacctcaccc gcgccgagca
     3961 ggaaatcctc gcagagcacg ccgaccggct gctggcggcg gcccaggccg aggaggtgct
     4021 ggagctgggc tccggctcct cccgcaagac acggctgctc ttggaggcga tgcgtcgtca
     4081 caacggcggg cggcgctacg cccccctcga cgtcagcgag gacgccctca tcggcgcggc
     4141 gctggcgctc tgcaacgact acccgtggct gcgcttcga
//
LOCUS       DASWDC010000039         4035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1426539, whole genome shotgun sequence.
ACCESSION   DASWDC010000039 DASWDC010000000
VERSION     DASWDC010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426539"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VF995_01120"
     CDS             <1..701
                     /locus_tag="VF995_01120"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003909497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9342202.1"
                     /translation="ALAAAGARLTLAAGNAAKLEAVGAEVKELGGEAVLVNRRTDELA
                     DAEAMVAAAVEAHGRLDQVVAAAGLNKVGLIADQDPADWDEVQRANVRGSWLLCKAAG
                     QRLIAQGEGGKVVLLSSTRGRLGLPSGYSAYCPSKAAVDLLTKTLACEWGRHRINVNA
                     IAPTVFRSDLTAWMYAEEGAGRATREAMLSRIPLGRLGEPEDFVGALLFLLSPASDFC
                     TGHVLYVDGGYTAG"
     gene            705..1628
                     /locus_tag="VF995_01125"
     CDS             705..1628
                     /locus_tag="VF995_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009836036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9342203.1"
                     /translation="MTPPGGKVAVVGAGLMGHGIAQVFAGAGWQVGIYDPVPEALASV
                     PARVRANLATTGQGEGDAVEALLGRIALHNDLAGAVGEAAYVTEAAPEDLALKRELFE
                     RLDALAPPEVVLATNTSVMSVGEIGSRARRRERVVGTHWWNPPYLIPLVEVTQAETTS
                     LETVEQTMALLAAIGKTPVHVRKDVPGFVGNRLQHALWREAIAIVAAGICDAETVDTV
                     VKSSFGRRLAVLGPLENADLVGLDLTLAIHEYVLPHIDHTPGPSPLLREKVAKGELGM
                     RTGQGLRPWPPGEADAVREHLLRYLTQSSGM"
     gene            1625..2254
                     /locus_tag="VF995_01130"
     CDS             1625..2254
                     /locus_tag="VF995_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HEX9342204.1"
                     /translation="MTTIPPIPMPLRWDAPPERWSVEDGDRDGGGGGAVLAVTAGPRT
                     DLFADPGGGPATLNAPRLLGDPAGDFQLSARVTVGFAAAFDAGVLLLWAHERAWAKLC
                     FESSPRGEPMVVSVVTRGVSDDCNAFTVDGDHVWLRAARLGPAFAFHASTDGSTWRFV
                     RYFTLDPAADAAAAAVGFEAQSPTGESCTVTFDAIRFTTERLSDLRDGT"
     gene            complement(2325..2666)
                     /locus_tag="VF995_01135"
     CDS             complement(2325..2666)
                     /locus_tag="VF995_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342205.1"
                     /translation="MRLSELLHAEVVDEHGRSAGRVQDLRLVQDGPLLGGFGAAFRAA
                     GLIVGGGGFGARLGYDRRSVKGPWPVKALISYLLRGARYVPWERIRSIEPDRIVIAGS
                     VDELSPPGTAT"
     gene            complement(2653..3042)
                     /locus_tag="VF995_01140"
     CDS             complement(2653..3042)
                     /locus_tag="VF995_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342206.1"
                     /translation="MSGRIIDAGLTLLDRQLLDSEQMLAGKIDDLELTIPEGGGPPVV
                     TAILAGPGALASRLGPRFGRLVEVIAARLRHPEEAPGRISFGLVKRIDSAVRLSVPRG
                     ELDTDRAESLARDHLVRHIPGSGHAPQ"
     gene            complement(3045..>4035)
                     /locus_tag="VF995_01145"
     CDS             complement(3045..>4035)
                     /locus_tag="VF995_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX9342207.1"
                     /translation="LLNLFASFIVNLLTLAAEIGGIALAVELLTGINYLIWVPPAALL
                     VWLVLWRMRFQLMENLFGLAGLALLVVVVALWRYHPDWGGLLHGAVHPAVPRGEGHPT
                     YWYYAIALLGSAMTPYEVFFFSSGAVEEGWSSGDLLVNRMNVYLGFPLGGALALALMV
                     SATLFLEPRGIDPSHLSQAALPVALVLGRVGLAFAIFAFFVLTFGAALETGLSSGYTI
                     AQYFGWQWGKYVKPAEAARFHLVMLASVVTGALLILTTIDPIKLTEYTLVLSAAALPL
                     TYFPILVVANDPDYMGSKVNSRFTNAAGSVLLVILVVTMLAAGPLMIVTKAGQ"
BASE COUNT          597 a         1465 c         1463 g          510 t
ORIGIN      
        1 tggcgctggc ggcggccggg gcgcggctca ccctggcggc cggcaacgcc gccaagcttg
       61 aggcggtcgg cgccgaggtc aaggagctcg gcggcgaggc ggtgctggtc aaccgccgca
      121 ccgacgagct ggccgacgcc gaggcgatgg tcgcggcggc ggtcgaggcc cacgggcgcc
      181 tcgaccaggt ggtcgccgcc gccgggctca acaaggtcgg cctgatcgcc gaccaggacc
      241 cggccgactg ggacgaggtc cagcgcgcca acgtgcgcgg ctcctggctg ctctgcaagg
      301 ccgccggcca gcgcctgatc gcgcagggtg agggcggcaa ggtggtgttg ctgtcctcca
      361 cccgcggccg cctcggcctg ccgagcggct acagcgccta ctgcccctcc aaggcggccg
      421 tggacctgct caccaagacg ctcgcctgcg agtggggccg ccaccgcatc aacgtcaacg
      481 cgatcgcccc gaccgtgttc cgctccgacc tgaccgcctg gatgtatgcg gaggagggcg
      541 cgggccgggc gacccgcgaa gccatgctgt cgcgcatccc gctgggacgg ctcggcgagc
      601 ccgaggactt cgtcggcgcg ctgctgttcc tgctgtcgcc cgcgtcggac ttctgcaccg
      661 gccacgtcct gtacgtggac ggcggctaca cggcggggta ggcgatgacg ccgccgggcg
      721 gaaaggtcgc ggtggtcggc gccgggctga tgggccacgg catcgcgcag gtgttcgcgg
      781 gcgcgggctg gcaggtcggg atctacgacc cggttcccga ggcactcgcc tccgtgcccg
      841 cgcgggtgcg ggcgaacctg gcgaccaccg gccaaggcga gggcgatgcg gtcgaggcgc
      901 tgctcggccg catcgccctt cacaacgacc tcgcgggcgc ggtcggcgag gccgcctacg
      961 tgaccgaggc agcccccgag gacctcgccc tcaagcggga gctgttcgag cggctcgacg
     1021 cgctcgcccc gccggaggtg gtcctggcga ccaacacctc ggtgatgtcg gtcggcgaga
     1081 tcgggtcccg cgccaggcgg cgcgagcggg tggtcggcac ccactggtgg aaccccccct
     1141 acctgatccc gctggtcgag gtcacccagg ccgagacgac cagcctggag acggtggagc
     1201 agaccatggc cctgctcgcc gccatcggca agacccccgt gcacgtccgc aaggacgttc
     1261 ccgggttcgt cggcaaccgc ctgcagcacg ccctgtggcg cgaggcgatc gcgatcgtcg
     1321 ccgccggcat ctgcgacgcc gagacggtcg acacggtggt caagagcagc ttcggccgcc
     1381 gcctggcggt cctcggcccg ctggagaacg cggacctggt cggcctcgac ctgaccctgg
     1441 cgatccacga gtacgtcctg ccgcacatcg accacacccc tggcccctcc cccctgctgc
     1501 gcgagaaggt cgccaagggc gagctcggca tgaggaccgg ccagggcctg cgaccgtggc
     1561 ccccgggcga agccgacgcc gtccgcgagc acctgctccg ctacctcacc cagtcgagcg
     1621 gcatgtgacc accatcccac cgatcccgat gccgctgcgc tgggacgcgc cgccggaacg
     1681 ctggagcgtc gaggacggcg acagggatgg gggcgggggc ggcgcggtgc tggcggtgac
     1741 ggccgggccg cggaccgacc tgttcgccga tccgggtgga gggccggcca ccctgaacgc
     1801 gccccggctg ctcggcgacc cggccgggga cttccagctc agcgcccggg tcaccgtcgg
     1861 gttcgccgcc gccttcgacg ccggggtgct gctgctgtgg gcgcacgagc gcgcctgggc
     1921 caagctctgc ttcgagtcct ccccgcgggg cgagccgatg gtcgtctcgg tggtgacccg
     1981 gggcgtctcc gacgactgca acgccttcac ggtcgacggc gaccacgtct ggctgcgggc
     2041 cgcgcggctc gggccggcgt tcgccttcca cgcctcgacc gacgggtcca cctggcggtt
     2101 cgtgcgctat ttcaccctcg acccggcggc ggacgccgct gcggcggcgg tgggcttcga
     2161 ggcgcagtca cccaccggcg agagctgcac cgtgaccttc gacgccatcc gcttcacgac
     2221 ggagcgtctc agcgacctcc gcgacggcac ctgagcggcg accagccagc gagtagaacg
     2281 ttgatcttgc cagactgtca atcaatcact tccaaaaatt tgagctaggt ggcggttccc
     2341 ggcggggaca gctcgtcgac cgagccggcg atcacgatcc ggtcgggctc gatcgagcgg
     2401 atccgctccc aggggacgta gcgggcgccg cgcagcaggt agctgatcag cgccttcacc
     2461 ggccaggggc ccttgacgga gcggcggtca tagccgaggc gggcgccgaa gccgcccccg
     2521 ccgacgatca gcccggcagc gcggaaggcg gcgccgaagc cgcccagcag cgggccgtcc
     2581 tggaccaggc ggaggtcctg gacgcggccg gcggaacggc cgtgctcgtc gaccacctcg
     2641 gcgtggagca gctcactgag gcgcatggcc gctccccggg atgtgccgga cgaggtggtc
     2701 gcgggccagc gactcggccc ggtcggtgtc cagctcgccg cgcgggacgc tcagccgcac
     2761 cgcgctgtcg atccgcttga ccaggccgaa ggagatccgg cccggcgcct cctccgggtg
     2821 gcgcaggcgc gcggcgatca cctcgaccag gcgcccgaac cggggcccga ggcggctggc
     2881 gagcgcgccc ggcccggcga ggatcgcggt caccaccggc gggcccccgc cctccggaat
     2941 ggtcagctcc aggtcgtcga tcttgccggc gagcatctgc tcgctgtcga gcagctgccg
     3001 gtcgagcagc gtcagccccg cgtcgatgat ccgtccgctc atcgtcactg ccccgccttc
     3061 gtgacgatca tcagcggccc ggcggcgagc atggtcacca ccaggatgac cagcagcacc
     3121 gagccggccg cgttggtgaa cctggagttg accttggagc ccatgtagtc gggatcgttg
     3181 gcgaccacca ggatggggaa gtaggtgagc ggcagcgccg ccgccgacag caccagcgtg
     3241 tactcggtga gcttgatcgg gtcgatggtg gtcaggatca gcagcgcccc ggtgaccacc
     3301 gaggcgagca tgaccaggtg gaagcgggcc gcctccgccg gtttcacgta cttgccccac
     3361 tgccagccga agtactgcgc gatggtgtag ccgctcgaca ggccggtctc cagcgcggcg
     3421 ccgaaggtga gcacgaagaa ggcgaagatc gcgaacgcca gcccgacccg gccgagcacc
     3481 agcgcgaccg gcagcgccgc ctgcgacagg tgcgacgggt cgatcccgcg cggctccagg
     3541 aacagcgtgg ccgacaccat cagcgccagc gccagcgccc cgccgagcgg gaagccgagg
     3601 tagacgttca tgcggttgac gagcaggtcg ccgctgctcc agccctcctc caccgcgccg
     3661 gaggagaaga agaagacctc gtagggggtc atcgcggagc cgagcagcgc gatcgcgtag
     3721 taccagtagg tcgggtgccc ctcacccctg gggaccgccg ggtggacggc gccgtgcagc
     3781 agaccgcccc agtcggggtg gtagcgccac agcgccacga ccaccaccag cagcgccagg
     3841 ccggccaggc cgaagaggtt ctccatcagc tggaagcgca tccgccacag caccagccac
     3901 accagcagcg ccgccggggg cacccagatc aggtagttga tcccggtgag cagctcgacc
     3961 gccagggcga tcccgccgat ctcggccgcc agggtcagca ggttcacgat gaaggaggcg
     4021 aacaggttga gcagg
//
LOCUS       DASWDC010000040         5265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1090193, whole genome shotgun sequence.
ACCESSION   DASWDC010000040 DASWDC010000000
VERSION     DASWDC010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090193"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /gene="aspA"
                     /locus_tag="VF995_01150"
     CDS             <1..418
                     /gene="aspA"
                     /locus_tag="VF995_01150"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937305.1"
                     /note="catalyzes the formation of fumarate from aspartate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX9342208.1"
                     /translation="MCEVLTQVSAQVIGNDTAITIGGLSGAFELNVYIPLMARNLLQS
                     IAYLAAATREFATRCVDGITANTDHARELADSTLVLVTALNPVVGYDEAAAISKEAAA
                     TGRSLREVALAHGVSADVLDKALDLRAMAHGNGFAG"
     gene            complement(446..1621)
                     /locus_tag="VF995_01155"
     CDS             complement(446..1621)
                     /locus_tag="VF995_01155"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEX9342209.1"
                     /translation="MHTIDLLLLLILALTAFSGWRRGFAVVTLGYLGLLVGLAIGAWA
                     AARIGLFVGASDSLQRLLVGIAVFFMIAAAFHAVGTYAGTRIRASLFRGEVAGGVDSA
                     GGAVVAVIVTAVALWFIALTLSGVPFSGLSRTLSDSAILRTIDRAAPRPPAALSQIRG
                     LLARSPFPDAFANLRPPSAAGQAPAAVNTTGVKRAQFATVQIESRGCGGLLFGSGWPV
                     ATDLVVTNAHVVAGTRDHRVITRAGGGALLATVVWFDPKRDLALLSVPNLGIRPLTTV
                     ASVPSGTLGAVIGYPGGGAEQTVGARVALKTIAIGRDIYSASVVRREIYVLNARVQKG
                     DSGGPVVDRSGRVLGVVFAASTLSGTEGYALTMTELQQVLTAMGGNRTPVGVGDCAA"
     gene            1785..2009
                     /locus_tag="VF995_01160"
     CDS             1785..2009
                     /locus_tag="VF995_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342210.1"
                     /translation="MEIEARRGAGEFLTLAAARALWRAPQRADACWADVRLPDGTVRA
                     CGRPEASSLGLCAQHRLELFGPPVHATVAP"
     gene            2057..2557
                     /gene="msrA"
                     /locus_tag="VF995_01165"
     CDS             2057..2557
                     /gene="msrA"
                     /locus_tag="VF995_01165"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX9342211.1"
                     /translation="MPTGTGTRSEKALFAAGCFWGVEAAFRMTPGVLATTAGYSGGTA
                     EHPTYQQVCSGRTGHAETVEVEFDPAKVSYAQLLDVFWKNHDPTQVNRQGPDRGTQYR
                     TAIFYLTPEQEAAAKASRDAEQERHTRPIATEITPASTFWKAEDYHQQYLEKRGLATC
                     VIPSRS"
     gene            2577..2939
                     /locus_tag="VF995_01170"
     CDS             2577..2939
                     /locus_tag="VF995_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX9342212.1"
                     /translation="MARLLFHCTHGQGDPERAILPFIAANVAVASGQEAIVLLTVDGA
                     WLAKQGYAETVAAEGFPKLAELLPAFVEGGGALWACSACTTPRGITEADLVPGATIVG
                     ATTIVEAVARGATPVALA"
     gene            complement(2941..3366)
                     /locus_tag="VF995_01175"
     CDS             complement(2941..3366)
                     /locus_tag="VF995_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9342213.1"
                     /translation="MPKRYPLPGPVARSLEVVGDRWTLLVIRELLHSPARYGELERAL
                     PGIPSNLLAERLRLLEDEGIAARDPAGGDRAYHLTPKGRELAPVLASLASWGIRHYGE
                     PSSALATHASCGGRVLTRSTCARCGAVVHAEELDLQSLR"
     gene            3439..>5265
                     /gene="cofG"
                     /locus_tag="VF995_01180"
     CDS             3439..>5265
                     /gene="cofG"
                     /locus_tag="VF995_01180"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEX9342214.1"
                     /translation="MPTITVTPDTPLDELLAEARRIRDQRFGGRVTYSPKVFIPLTML
                     CRDHCGYCTFAKPPARLDRGAYLTPDAVLEIVRAGAAAGCHEALFTLGERPEERYPAA
                     REALAALGYGSTVEYLAAMCRLVIEETGLLPHANAGALFPDELASLRQVSASQGMMLE
                     SLSERLHEPGGPHARCETKVPARRLATLEAAGELRIPFTTGILVGIGERRAERLEALA
                     AIDAAHRRHGHVQEVIVQNFRPKPGTAMAGSPAPSLEDYRWTIAAARVLLDPAIHLQA
                     PPNLTEDFGVLLEAGIDDWGGVSPITADHVNPEAPWPALERLRAVTEAADRTLAPRLT
                     IYPEFAADPDRWLDDALRFPVLDRSDSEALGRDSRWSAGDLEPPPSLIDAAPFPTPTP
                     TAGGPVGEALAGARAGQELGEEQIVALFRARGPQVRAVADFADELRRQAAGEVVTFVR
                     NRNINYTNVCTFRCRFCAFSKGPLSLNLRGAPYLLGLDEVAARVVEADARGATEVTLQ
                     GGIHPSFDGDYYIEVARAVHEAVPGMHIHGFTALEVHEGARRLGEPLRDYLERLRAAG
                     LKSLPGTAAEILDDEVRAVICPDKITTDEWLEVHRTAHSIGLR"
BASE COUNT          772 a         1884 c         1938 g          671 t
ORIGIN      
        1 catgtgcgag gtgttgaccc aggtctccgc ccaggtcatc ggcaacgaca ccgccatcac
       61 catcggtggc ctctccggcg ccttcgaact gaacgtctat atccccctga tggcccgtaa
      121 cctgctccag tcgatcgcct acctcgccgc cgccacccgc gagttcgcca ccaggtgcgt
      181 cgacggcatc accgccaaca ccgaccacgc ccgcgagctg gccgactcca ccctggtgct
      241 ggtcaccgcc ctcaacccgg tcgtcggcta cgacgaggcc gccgccatct ccaaggaggc
      301 cgccgccacc ggccgctccc tccgcgaggt cgctctcgcc cacggggtga gcgcggacgt
      361 cctggacaag gccctggacc tgagggcgat ggcacacggc aacggtttcg ccgggtaaag
      421 cggctcgcgc tccctcgata ggaagttaag cagcgcaatc accgacgccg acgggggtgc
      481 ggttgccgcc catggcggtg aggacctgct ggagttcggt catggtgagg gcgtagccct
      541 cggtgccgct gagggtggag gcggcgaaga cgacgccgag gacgcggccg gagcggtcga
      601 cgaccgggcc gccgctgtcg cccttctgga cccgggcgtt caggacgtag atctcgcggc
      661 ggacgacaga ggcggagtag atgtcgcggc cgatggcgat cgtcttgagg gcgacccggg
      721 cgccgacggt ctgctccgcg ccgccgccgg ggtagccgat gaccgcgccg agggtcccgc
      781 ttgggaccga ggcgaccgtg gtgagcggcc tgatgccgag gtttggcacg ctgagcaggg
      841 cgaggtcgcg tttcgggtcg aaccagacga cggtggcgag cagggcgccg ccgcccgccc
      901 gggtgatgac acggtggtcg cgggtgccgg cgaccacatg ggcgttggtg accacgaggt
      961 cggtggcgac cggccagccg ctgccgaaca ggaggccgcc gcaacctcgg ctctcgatct
     1021 ggacggtggc gaactgggcg cgcttgacgc ctgtggtgtt gaccgccgct ggtgcctgcc
     1081 cggcggccga ggggggccgc aggttggcga aggcatccgg gaagggcgag cgggccagca
     1141 ggccgcggat ctgtgacagc gcggccggcg ggcggggcgc ggcgcggtcg atggtccgca
     1201 ggatggcgga gtcggacagg gtccggctga gccctgagaa cggcacgccg gacagggtga
     1261 gggcgatgaa ccagagcgcg accgcggtca cgatcaccgc caccaccgcg ccgccagccg
     1321 agtcgacccc gccggcgacc tcaccccgga acagggaggc gcggatccgg gtgccggcgt
     1381 aggtgccgac ggcgtgaaag gcggcggcga tcatgaagaa caccgcgatc cccaccaaca
     1441 ggcgctggag cgagtcgctc gcgccgacga acaagccgat ccgcgcggcc gcccaagcgc
     1501 cgatggcgag gccgaccagc aggccgaggt agccgagcgt caccaccgcg aacccccgcc
     1561 gccagccgct gaacgcggtg agggccagga tgagcagcag cagcaggtcg atggtgtgca
     1621 tcgggggttc ggtgtccttc cggtgtcagg tacagaccac acgggcctgg caagggtccc
     1681 tcattcaagc gtaaggcctc ccgggtgtca tgccgatgca gaggcaatga caggacgggc
     1741 tcaaatgggc ccggaaccac ttctttggca cctggagaaa cccgatggag atcgaagcgc
     1801 gacgaggcgc tggggagttc ctgacgctgg cggcggcgcg ggcgctgtgg cgggcgccgc
     1861 agcgggccga cgcctgctgg gcggacgtgc ggctgccgga cgggacggtg cgggcctgcg
     1921 gccggccgga ggcgagcagc ctcgggctgt gcgcccagca ccgcctggag ctcttcggtc
     1981 ccccggtcca cgccaccgtg gcaccgtagg ggtaccgtga ggtgtgggat gccagcagct
     2041 tgaggagggc tcagccatgc cgaccgggac cgggaccagg tccgagaagg cactgttcgc
     2101 cgcggggtgc ttctgggggg tcgaggccgc gttccggatg acccctggcg tgctcgccac
     2161 caccgccggc tacagcggcg ggaccgctga acaccccacc taccagcagg tctgcagcgg
     2221 ccgcaccgga cacgccgaga ccgtcgaggt cgagttcgac ccggccaagg tctcctatgc
     2281 gcagctgctg gacgtcttct ggaagaacca cgacccgacc caggtgaacc gccagggccc
     2341 ggacaggggc acccagtacc gcaccgcgat cttctacctc acccccgagc aggaggccgc
     2401 cgccaaggcg tcgcgggacg ccgagcagga acgccacacc cgcccgatcg ccaccgagat
     2461 caccccggcc tcgaccttct ggaaggccga ggactaccac cagcagtacc tggagaagcg
     2521 cggcctggcg acctgcgtga ttccctcgcg gtcctgaacc gggcggagct gagctgatgg
     2581 cgcggctgct gttccactgc acccacgggc aaggcgaccc ggaacgggcg atcctgccgt
     2641 tcatcgccgc caatgtggcc gtcgcctccg ggcaggaggc gatcgtgctg ctcaccgtcg
     2701 acggcgcctg gctcgccaag cagggctatg ccgagacggt cgccgccgag gggttcccga
     2761 agctggccga gctgctcccg gcgttcgtgg agggtggggg cgcgctctgg gcctgcagcg
     2821 cctgcaccac cccgcggggg atcaccgagg ccgacctcgt gcccggcgcc acgatcgtcg
     2881 gggccacgac catcgtggag gccgtggcgc ggggtgccac gccggtcgcg ctcgcctgac
     2941 ctaccgcagc gattggaggt ccagctcttc ggcgtggacg accgcgccgc agcgggcgca
     3001 ggtggagcgg gtcaggaccc ggccgccgca ggaggcgtgg gtcgctaggg cggaggacgg
     3061 ctcgccgtag tggcggatgc cccagctggc caggctggcc agcactgggg cgagctcgcg
     3121 gcccttcggg gtcaggtggt aggcgcggtc gccgccggcc gggtcgcggg cggcgatccc
     3181 ttcgtcttcg agcaggcgca ggcgctcggc cagcaggttc gaggggatcc cggggagggc
     3241 gcgctccagc tcgccgtagc gggcggggct gtggagcagc tcgcgaatca ccagcagggt
     3301 ccagcggtcc cccacgactt cgagggagcg ggcgacgggt ccggggagcg gatagcgttt
     3361 gggcacgaat ggaccgtagc acttgagcgg ctacaaagtt cacttgactt tctgaagtgg
     3421 agccgggacc ctggcggcat gccgacgatc acggtcacgc cggacacccc gctcgacgag
     3481 ctgctggccg aggcgcggcg catccgcgac cagcggttcg gcggccgggt cacgtactcg
     3541 cccaaggtct tcatcccgct caccatgctg tgccgcgacc actgcggcta ctgcaccttc
     3601 gccaagccgc ccgcccggct ggaccggggc gcctacttga cgcctgacgc ggtgctggag
     3661 atcgtccgtg ccggggcggc ggccggctgc cacgaggcgt tgttcaccct cggggagcgg
     3721 ccggaggagc gctacccggc ggcgcgggag gcgctggccg cgctcgggta cggctcgacc
     3781 gtcgagtatc tggcggcgat gtgccgcctc gtgatcgagg agaccgggct gctgccgcac
     3841 gccaacgccg gggcgctgtt ccccgacgag ctggccagcc tgcgccaggt cagcgccagc
     3901 caggggatga tgctggagtc gctctcggag cggctgcacg agcccggcgg gccgcatgcc
     3961 cgctgcgaga ccaaggtgcc cgcccgccgc ctcgccactc tggaggccgc cggggagctg
     4021 cgcatcccct tcaccaccgg gatcctggtc gggatcggcg agcgccgcgc cgagcggctg
     4081 gaggcgctcg ccgcgatcga cgccgcccac cgccgccacg gccacgtcca ggaagtgatc
     4141 gtccagaact tccggccgaa gcccggcacg gcgatggcgg gttcgcccgc gccgtcgctg
     4201 gaggactacc gctggacgat cgcggccgcc cgggtgctgc tggacccggc gatccacctg
     4261 caagccccgc cgaacctcac cgaggacttc ggggtgctgc tggaggccgg catcgacgac
     4321 tggggcgggg tcagcccgat caccgccgac cacgtcaatc cggaggcgcc ctggccggcg
     4381 ctggagcggc tgcgcgcggt caccgaggcg gccgacagga ccctcgcccc gcgcctcacc
     4441 atctacccgg agttcgccgc cgacccggac cgctggctgg acgacgccct gcgcttcccg
     4501 gtgctggacc gctcggacag cgaggccctc ggccgcgaca gccgctggtc cgccggggat
     4561 ctggaacccc cgccctcgct gatcgacgct gcccccttcc cgacgccaac gcccacggcg
     4621 ggcgggccgg tgggggaggc gctcgctggc gcccgcgcgg gccaggagct gggcgaggag
     4681 cagatcgtcg cgctgtttcg cgcccggggc ccacaggtgc gggcggtcgc cgacttcgcc
     4741 gacgagctgc gccgccaggc ggccggcgag gtggtcacct tcgtccgcaa ccgcaacatc
     4801 aactacacca acgtctgcac cttccgctgc cgcttctgcg ccttctccaa ggggccgctg
     4861 agcctcaacc tgcgcggggc cccctacctg ctcgggctgg acgaggtcgc ggcccgggtg
     4921 gtggaggccg acgcccgcgg cgccaccgag gtcaccctcc agggcggcat ccacccgagc
     4981 ttcgacggcg actactacat cgaggtcgcc cgcgcggtcc acgaggcggt cccgggcatg
     5041 cacatccacg gcttcaccgc cttggaggtc cacgagggcg cccgccgcct cggcgagccg
     5101 ctgcgcgact acctggagcg cctccgcgcc gccgggctga agagcctgcc gggcaccgcc
     5161 gccgagatcc tcgacgacga ggtccgcgcg gtcatctgcc cggacaagat caccaccgac
     5221 gagtggctgg aggtgcaccg caccgcccac tcgatcggcc tgcgg
//
LOCUS       DASWDC010000041        21731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_510260, whole genome shotgun sequence.
ACCESSION   DASWDC010000041 DASWDC010000000
VERSION     DASWDC010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 21731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21731
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510260"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..870)
                     /locus_tag="VF995_01185"
     CDS             complement(55..870)
                     /locus_tag="VF995_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX9342215.1"
                     /translation="MPEGDTVHLLAAKLDAALAGQRLVATDFRVPRFATRDLAGQDVA
                     AVVAHGKHLLLRTDAGVTLHSHLKMEGAWHLYRPDERWRGPDFQVRAVLRTDPWVAVG
                     FRLGTCELLPTSREHEVVGHLGPDVLGPDWDLAEAARRLRADPDRAIGTALLDQRAVA
                     GPGNIWKCEICFLRGVDPWTQVGQVADLEGMLALLKRMMEANRTIGHQVTTGDPRPGR
                     SHWVAERAGKPCRRCATPIRKAPQEGYGGERVTWWCPSCQQRPALDYEHAGRC"
     gene            complement(857..5458)
                     /locus_tag="VF995_01190"
     CDS             complement(857..5458)
                     /locus_tag="VF995_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9342216.1"
                     /translation="MNTTPAPITPNTPLEHFSPLTRAWFRDAFAAPTAAQAGAWAAIA
                     GGQDALVVAPTGSGKTLAAFLSAIDRLATAPPVPARERLRVLYVSPLKALAADIERNL
                     RTPLAGLRAAAARLDLPLPQLSVAMRTGDTPADARRRFAQAPPDILITTPESLFLLLT
                     SQAREALRHLDTVIVDEVHSVVATKRGAHLAVSLERLDELLERPAQRIGLSATVRPLD
                     EVARFLSGGRPVTVVAPPSQKTFDLSVVVPVEDLAAIGAEPSRGSDADDADGVEGAAP
                     ERTGIWPHIEERLLDLIRAHRSTIVFANSRRLAERLCASLNDLAGAEIARTHHGSVSR
                     EQRVQTEEDLKAGRLPAVVATSSLELGIDMGTVDLVVQVEAPTSVAQGLQRIGRAGHQ
                     VGATSRGMVFPKFRGDLVECAVVVERMHAGAIEAMRYPRNPLDVLAQQVVAMVAMDDW
                     PLDRLEALLRRAATYAELPRSALESVLDMLSGRYPSDEFAELRPRLNWDRGTDVLSGR
                     PGAQRLAVTSGGTIPDRGLFGVYLVGEGQARVGELDEEMVYESRVGEVFTLGASSWRI
                     EDITHDRVLVSPAPGQPGKLPFWHGDAPGRPLELGRALGAFLRELVALDPATRLERLS
                     GAGLDHNAASNLLAYLGEQLEATGVLPDDRTIVVERFRDELGDWRVCVHSPFGAPLHA
                     PWAQAIEARVRERLGLEVQTMYSDDGIVVRLPEADEAPPSDSILFEPEEVDDLVVGQV
                     SGSALFASRFRECAARALLLPRRRPGRRTPLWQQRQRSAGLLQVAAGYGSFPIVLEAM
                     RECLQDVFDLPGLAELMAAVGRREVRVVEVDTPFPSPFASSLQFGYVAAFMYEGDAPL
                     AERRAQALSLDRTLLAELLGLQELRDLIDPAALADLELELQLLTPERRVGDPDRLHDA
                     LRLLGDLTVAEATARAADPAALPGWLAELEGTRRALRLRIAGEERWAAIEDAARFRDA
                     LGTAMPPGVPTAFLDQVADPVGDLVGRFARTHGPFLAADVARRLGLGVAVVEQTLIRL
                     EGAGRVVAGEFRPGGAGREWLDAEVLRRLRRRSLAALRKEVEPVPQAALARFLTAWQG
                     VAPVASRGRVAALDAVGRVIEQLQGVAVPASALEWQVLATRLPGYEPGLLDQLCASGE
                     VVWAGAGALGGDDGWVSLYLAEQAPLLLPDPLPAELSPLAVALHEALEDRGALFFRQL
                     SDAVATSTSTTSSGGAGSGNDTELLLAIWELVWAGKVTNDTMAPLRALVSGGVRAPGR
                     PPRLPRAGRPRRPAMPSRLGPPAGAGRWSLVPERSSDATRRLHAAAEQLLERHGVVTR
                     GAVVAERIPGGFAGVYAVLKAMEESGRCRRGYFVDGLGGAQFAVPGAVDRMRALEGPA
                     ERDPHPQTLVLAAADPANPYGAALAWPDRAVGADGNLADRPGHRPGRKAGAVVVLVDG
                     ALVLYVERGGRTLLTFDDHPDHLRRAADALALAVRDGALGRMAVERADGETVFDSPLA
                     QALTEAGFRPTARGLRILGRPGSASLGPGHGGARPDAGR"
     gene            5621..9343
                     /locus_tag="VF995_01195"
     CDS             5621..9343
                     /locus_tag="VF995_01195"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX9342217.1"
                     /translation="MGWFDRPEFGPNIGLIDEIYRKYLDDPTSVSEAWQEFFAEQEPE
                     DTDGDSDSDGAAPATVATPTAPPEPPPARPANAAAAPATAEAAKAEPPKAGPAADGDG
                     DSNGVRPVPLRGAAARIVEAMEASRAVPTATSFRVVPARLLEVNRNVLNGHLRRHRGG
                     KVSFTHLIAYAAVRALQALPAMTSIYQEHAGKPQVLRPEHVNLGLAVDTRRPDGSRTL
                     LVPNIKQADRLDFASFLRAYEELIAKVRTNKLAVADFQGTTVTITNPGTVGTVMSVPR
                     LLAGQAAIIGVGVIGYPAEYLGADPETLAELGIGKTVTLTSTYDHRVIQGAESGEFLA
                     RMHQLLLGSDGFYEEVFRSMGVPYVPVRWHADARAEDDLEATEKQARVYQLINMYRVR
                     GHLIADLDPLEVKPPSMHPELDPASFGLTIWDLERRFLTGGLCRERELPLAEILRILR
                     DAYCRTASVEYMHSSQADEKAWIQERVEVPPATLPKDDQLQILNKLIEAEVFERFLHN
                     KYVGQKRFSLEGAESLIPMLDAVLDRAADAAMDGVVVGMSHRGRLNVLANTVGKSYGE
                     IFHEFEGHLDPALAQGSGDVKYHLGAAGKHTARSGRQVRIEVASNPSHLEAVNPVAEG
                     LVRAKQDLLDRGEEAPVLPLLLHGDAAFAGQGVVAETLNLSQLRGYRTGGTVHIIVNN
                     QLGFTTSPDYGRSSTYATDVAKMIQAPILHVNGDDPEACVRVARLAFDYRQSFKKDVV
                     IDMWCYRRFGHNEGDEPAFTQPLMYARIKARPSVRKLYTEALVNRGDLSLEEAERSLE
                     AYRQRLQQAFDESHEHDQEGGGGDDDGEDGGSLPGASSVERRTGSLASDPPVETAVGQ
                     DRLHQIAAKLASVPDEFHVHPKLIRWLRDRSEALGKNGGVVDWSLAEALAFGSLLLDG
                     RIVRLSGQDTRRGTFSQRHSVLVDQENGEQYTPLANLAPDQGRFFVYDSLLSEFAALG
                     FEYGYAVANPDALVLWEAQYGDFVNGAQTIVDQFIVAAEPKWGQRCGLVLLLPHGYEG
                     QGPEHSSSRVERFLQLAADDNLQVAVPSTPAQYFHLLRRQALRATRKPLVVLTPKSLL
                     RLPAARSTAAALSDGRFHEILADPTNPDPQQVRQVLLCSGKVSYDLAKRRADQGADQG
                     AGAVAIVRVEQLYPLPAEQLRAQLDAYPNADRVLWVQEEPENMGAWRHLRLHARRALG
                     IELKGVTRPAAAAPATGSPRIHQREQQEVLDRAFAE"
     gene            9423..9827
                     /locus_tag="VF995_01200"
     CDS             9423..9827
                     /locus_tag="VF995_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9342218.1"
                     /translation="MKVRQVASSAVVTVGPMQSLREAAQVMSRHRVGSAVVQDAEQLI
                     GILTERDVLNAVAGGATPEDVSVQEIMTADVVTVGPDWDLLEAAGEMARRRIRHLVVY
                     DGRQLLGVLSVRDVLPAILPDVSTSADARGLG"
     gene            9865..11226
                     /locus_tag="VF995_01205"
     CDS             9865..11226
                     /locus_tag="VF995_01205"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX9342219.1"
                     /translation="MDLDEVLAKIAADEVEFVRFEQTDLHGISRSKTIPARHVGAFAR
                     DGLNFLLGQLGLDAQGGVAPGTGYLEERGFPDSRLFPDPETYAVLPWAVATARLLCDP
                     HFIDGTPAPASPRLVMQRLCQDLADEGFLHLSGYEYELYLVRADDRQPPFPGIQIFAT
                     LRNTFDEAFVSDVLRAMPRVGVDIITANAEYGPGQLEINFAPAIGVAAADQAFTFKNG
                     IKEIARRHGYLASFMTKPYADQSASGCHLHESLLDRETGRNAFAVDDTTPGKLSDVGR
                     WWIGGQLAHLPALTAFFAPTVNCGKRFKPWSFAPVNRTWGLENRTVAIRVKGLAGERL
                     HLENRVPCGASNPYLVGAAAIAAGLDGIRNRVEPPPETDGLAYGLEGPALIPTRLEAA
                     LDELEKDMALRGLLGEELIRLFLAVKRHEVAKAGDHARDYAAGDWADRVDEFEVAGLF
                     EFL"
     gene            complement(11242..12726)
                     /locus_tag="VF995_01210"
     CDS             complement(11242..12726)
                     /locus_tag="VF995_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342220.1"
                     /translation="MRHHKERFRQLAVTLALGLVVAAFAAPTPALAQATATATTTTTA
                     RPDAATEIHFTWLQGFDDPATPASLNRVGVLKVGSDRARNVLVLSPGTSAGAGYFLPL
                     ARDIVRRTPGRWQVWSVERRENQLEDQSVLTQAKQAKATPQQLFDYYLGWLVDPGITT
                     HFQPIPDAAVPFARGWGMNVEIQDLHRVVEAAGQHGRRVVLGGHSLGGTITTAYATWD
                     FNGSPGARGLSGLVYIDGGSNPAPISADQATQALQNLQASSPWLAFGGIPAPFLGLFA
                     ATGSTAAVIDPHGLSLAQSFPLLPADLRPPVPTTNEAQFGFGVDTETSPPALVAAQVH
                     AGHLAASGTPRGWVRAGELTPIQRYARMLSGTSLAGVDGSAWYHPLRLTIDAGAVADG
                     NANPAQAILGVDAIHGHDLGAHLPIYAFGAALGGQGVLDTAAILAQQSSIPSSDLTLI
                     NRQATYAHNDPAAASPNNEFLDNLIPFLARVAHQAATPPPATVP"
     gene            complement(12952..14088)
                     /locus_tag="VF995_01215"
     CDS             complement(12952..14088)
                     /locus_tag="VF995_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9342221.1"
                     /translation="MAAAFRSEIAALERLPGASQDDVLEGVRRSLLRWWRWLILGVAP
                     PDEEFAPLRAWARARAGEGVRLEDLLRAFGIGGQVGWELIRRHARADEHDALLDAAGL
                     LMRYVDRISAVVADTYLAERDLLLSEEERQARDLLERLTRGDAAPLDLGDRELAERLG
                     VTVEEAYAPFVVLLPGQPLRRHAALAARLRRRGWKLAVTDGARVVGLTSATRPLDVAD
                     LGEGPDVLLVTASAAPRDDAALADAREDVAALAEHGWRAGLRGRLRAEEHPVELLVAR
                     SPGPAARLRARVLWPLAAPDHEELLRTLRAFVAHQYDRAATSEALHVHRNTLAYRLRR
                     IERLTGLDLTTARDLAGVYLAVASTPSPGPGPGGPATSERRPQP"
     gene            14312..15748
                     /locus_tag="VF995_01220"
     CDS             14312..15748
                     /locus_tag="VF995_01220"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX9342222.1"
                     /translation="MTNATAQEDQQAHQRTAAARVLELAEASGLALVRFLYVDHGGVV
                     RGKASGRHGLGGRLRSGIGLTLAMQAMTMLDELQPVEGTGPVGEIRLVPDPESFVVLP
                     YAPGAGAMLADMVRLDGEPWAACPRGFLKQAVAALAAEGYALQAAFEPEFTLGRRVLA
                     PDAPAASGGAPGLERLEPLDRSLCFSTTGFDAAHGYVIELVRALEAQGLVVEQYYPEL
                     GWGQQELSIGYAPALRAADNQVLYRDTARGVAARQGLWATFAPKPIPDQAGNGCHLHA
                     SLWDAALRRNLFHDASDRYGLSELAYHFVGGLLAHLPALVALTCGSVNSYRRLGPRSW
                     SSAYRCYGPDNREAAVRIASPMWGAEEASTNLELKCSDATGNPYLALGAYIHAGLDGI
                     RNKLDPGEAADADPDTLPAAVRRRRGIDRLPDSLPAALDALAADKLLMEALGPLRRDA
                     YLRVKRAEAAAFAAGDAAFECYHHFMKF"
     gene            15801..17381
                     /locus_tag="VF995_01225"
     CDS             15801..17381
                     /locus_tag="VF995_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9342223.1"
                     /translation="MATVEPVAGGELLSERVAGGILPKVLNSFDMVAIFLAIVLFITN
                     AAVIQSAGPAAFGWWILGFVAFLIPGAIVTGQLGLMFPGEGSIYLWTHKAFGAFWGFF
                     AGFCAWWPGVLVMVATGVGTLAFLGFVFPGTVGAWSVKTQGTAIIGFIVLSALLAVQR
                     FRVTQNIVNVVVVLYGLAILLMLLAGIVHLAGGHAAEVSPAQLSTWAPGSKSGINFTN
                     WTFFGLVILALLGVEVPLNMGVEIREVRSITRYLFWGSIGVMVAYLVATWAVMVTVPA
                     ASGQSAAPVAAAAVKISLGTFWGDLVGLILAGFFLFITVVYNYSFARLIFVSGLDRRL
                     PRALSRVNAAKVPDVAVWTQSCIAALFTLVAFVILPVASGGAKQVDVQTKVYDILQAA
                     VTVIWCVSMVVLFIDVLVIVRRYAGVFAARRLGGAANAGVFWVCSVIGALASLIGIVA
                     TLSGSWTPLISNDAGTVSVFGAQITYGDWFYWVGGIALASMVVGALLYLLGRRTAASA
                     ARSGSGSGTTLPASEAGG"
     gene            complement(17415..18011)
                     /locus_tag="VF995_01230"
     CDS             complement(17415..18011)
                     /locus_tag="VF995_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342224.1"
                     /translation="MPQRSTGPAGPRDRGVLIGLAFIGLVLVLLFCGGALGVLITKGT
                     ATTTATPPASAPPSSGDQAMRTLQAAVVQRFRHLMQIRQVALLRRDAAMLAAIYTPNS
                     PNLRRDRHEIDALLQAHKRWVDLTLPVGVLHAYQVSSGRWMVVAVLGRSGARLVTDDG
                     QLLRDVPGAEAVYWCTLQRVQGAAAGGAFRLYQMLGTT"
     gene            complement(18035..19705)
                     /locus_tag="VF995_01235"
     CDS             complement(18035..19705)
                     /locus_tag="VF995_01235"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX9342225.1"
                     /translation="MTAAPSWWVEPFTEEERAILAPWFTNTDRPVFALRNLPEAVKGA
                     LFSRYSRSGKSLRRLFLDEFAGELPTTPPTSGPVGEARAAALYHRVLDQYGDDSVAQL
                     GGAHVACEQASNLLTKALEWGRLAAYLEQSTRYVSYTDRPGGRWRYHLDPDVDADPAL
                     GPEYRRVLDGLFTAYSELVPALIAVLAERLPRDPAASEPAWRRAVKARALDGLRGLLP
                     AATTSNVGIYASAQSYEALLLRLRAHPLPEARAAAELLLRELRQVIPVFLTRVDRPDR
                     GVAWSRYLAETAEETAKVVDALLGDPDRGVGDQAPSVRLVEFDPAAEDKILTAIAYPH
                     LRVGEEAVAARVARLTTEERARLLAAYVGRRGNRRHRPGRAFERALYTFDVVADYGAF
                     RDLQRHRMCTIVWQPLRPDLGYDVPADVEAAELGDRFAAVMRASASLYRALEPRFPAQ
                     APYAVSLAHRVRFAITLNARELLHLAELRSEPQGHPAYRLVAQRMHRLIAERAGHHGL
                     AAAMRFVVHDEEDAGPGGDSGLGRLAAEERTEARRGGSGGTGSEGMPP"
     gene            complement(19702..20502)
                     /gene="dapB"
                     /locus_tag="VF995_01240"
     CDS             complement(19702..20502)
                     /gene="dapB"
                     /locus_tag="VF995_01240"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011210414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX9342226.1"
                     /translation="MRIGVLGATGRMGQATCRAVAATDDLELVAAVAGQRGSGQDLRA
                     LVAEAPGGLVVGGAVADLLAAKAEVVVDFSTPAATMAAARALLPEGVQLVSGTTGLAD
                     GAMEELRSLAGPSAGNAVWAPNFALGAVLATYFATIAARHYPAAEVIELHHAGKADAP
                     SGTAIRTAQAIAAARQGPDQRQPPPSRESVSGARGGEVDGVRVHSVRLPGLVAHQEVI
                     FGGQGELLTVRHDSLDRVSFMPGVLLAVRAVAARPGLTIGLEPLLGLA"
     gene            20767..21483
                     /locus_tag="VF995_01245"
     CDS             20767..21483
                     /locus_tag="VF995_01245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9342227.1"
                     /translation="MLASVDNEAVVMAVLADRFRLPMDRLVPGEVAIAEVEHPSLPIR
                     AFSREGGAVLAVAPRHAPLLRRYLHDPATLLPALEAVAARLPGHLFSGVARTGVAIPP
                     PAAEVTVLDATDPRLPDWVHGHFTGEAWAVLDDHSEVLSTAVLKRYDDRLREISVGTT
                     EAARGRGLARAVVAAAARAVLSEGRAVLYNHAPDNYASARVAESVGLHEFGRYHAIVP
                     DRRLGAEYGEDDTTAEADNR"
     gene            complement(21510..>21731)
                     /locus_tag="VF995_01250"
     CDS             complement(21510..>21731)
                     /locus_tag="VF995_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEX9342228.1"
                     /translation="ALLEGVARAHALVDGNKRLAWSATVVFYGLNGYDVEPPSTAEAV
                     EFVVGVVTAHPELDKIARKLGSWKRQRSP"
BASE COUNT         2972 a         7819 c         8033 g         2907 t
ORIGIN      
        1 gaaggctccg ccaacaagat cgggcaactt cgataactca gcaggtagcg gggctcagca
       61 gcgaccggcg tgttcgtagt cgagcgccgg ccgctgctgg cagctcgggc accaccaggt
      121 gacgcgctcg ccgccgtagc cttcctgggg ggccttgcgg atcggggtgg cgcagcggcg
      181 gcagggtttg ccggcgcgtt cggcgaccca gtgggagcgg cccggacgcg ggtcgccggt
      241 ggtgacctga tggccgatgg tgcggttggc ctccatcatg cgtttcagca gggcgagcat
      301 gccctcaagg tcggcgacct ggccgacctg cgtccaggga tcgacgccgc gcaggaagca
      361 gatctcgcac ttccagatgt tcccgggacc ggcgacggcg cgctggtcga gcagggcggt
      421 gccgatcgcc cggtctgggt cggcgcgcag gcgccgtgcg gcctcggcga ggtcccagtc
      481 ggggccgagc acgtccgggc cgaggtgccc gacgacctcg tgctcccggc tggtgggcag
      541 cagctcgcag gtgccgaggc ggaacccgac cgcgacccac gggtcggtgc gcagcaccgc
      601 ccggacctgg aagtcaggcc cgcgccagcg ctcatccggg cggtagagat gccaggcgcc
      661 ctccatcttg aggtgggagt gcagggtgac tccggcgtcg gtgcgcagca gcaggtgctt
      721 gccgtgtgcg accaccgccg ccacgtcctg cccggcgagg tcgcgggtgg cgaagcgtgg
      781 cacccggaag tcggtggcga ccagccgctg gccggccagc gcggcgtcga gcttggcggc
      841 gagaaggtgg acggtgtcac cttccggcat cgggccgtgc tcctccgtgg ccggggccga
      901 ggctggcgct tcccggccgg ccgaggatcc gcaggccgcg ggcggtcggg cggaagcccg
      961 cctcggtcag ggcctgggcg agggggctgt cgaacaccgt ctcgccgtcg gcacgctcga
     1021 ccgccatccg cccgagtgcc ccatcgcgga ccgccagggc gagcgcgtcg gccgcgcgcc
     1081 gcaggtggtc cgggtggtcg tcgaaggtga gcagggtgcg cccgccccgc tcgacataca
     1141 gcaccagcgc cccgtcgacc agcaccacca ccgcgcctgc cttgcgcccc ggccggtgcc
     1201 cgggtcggtc cgccaggttg ccgtcggcgc ccaccgcccg gtcaggccag gccagcgccg
     1261 ccccgtaggg gttggcggga tcggcggccg ccagcaccag cgtctgtggg tgcgggtcgc
     1321 gttctgccgg tccctccagg gcgcgcatgc ggtcgaccgc gcccgggacg gcgaactggg
     1381 cgccgccgag gccgtcgacg aagtagccgc ggcggcagcg gccggactcc tccatggcct
     1441 tgagaacggc gtagacgccg gcgaagcccc cggggatacg ctcggcgacc accgcgccgc
     1501 gggtgaccac gccgtggcgt tccaggagct gctcggcggc ggcgtgcagg cgccgagtgg
     1561 cgtcgctgga ccgctccggg acgaggctcc agcggcccgc gcccgccggc gggccgagcc
     1621 ggctgggcat ggcgggccgc cgtggcctgc ccgcccgggg caggcgtggc gggcggccgg
     1681 gtgcccggac gccgccggag acgagtgccc gcaggggtgc catggtgtcg ttggtgacct
     1741 tcccggccca gaccagctcc cagatggcga gcagcagctc ggtgtcgttg ccggagccgg
     1801 cgccgccgct gcttgtggtg ctggtgctgg tggcgaccgc gtcggagagc tggcggaaga
     1861 acagcgcacc ccggtcctcc agcgcctcgt gcagcgccac agccagcggt gaaagctcag
     1921 ccgggagcgg gtcggggagc agcagcggtg cctgctcggc caggtagagg ctgacccagc
     1981 cgtcgtcgcc gccgagcgcg ccagcacccg cccagaccac ctcgcccgag gcgcacagct
     2041 ggtcgagcag gccaggctca tagccgggca gccgcgtggc gagcacctgc cactccagcg
     2101 ccgacgcggg taccgccacg ccttggagct gctcaatgac ccggccgacc gcgtcgaggg
     2161 cggccaccct gccgcgggac gcgaccggag cgaccccctg ccaggcggtc aagaagcggg
     2221 ccagggccgc ctgcgggacc ggctccacct ccttgcgcag ggcggccagc gagcggcggc
     2281 gcagccggcg cagcacttcc gcgtccagcc attcccgccc ggcgccgccg gggcggaact
     2341 cccccgcgac cacccggccg gcgccctcca gccgaatcag ggtctgctcc accacggcga
     2401 cgccgaggcc gaggcggcgg gccacgtcgg cggccaggaa cgggccgtgg gtgcgggcga
     2461 aacggcccac caggtcgccg accggatcgg cgacctggtc caggaaggcg gtcggcaccc
     2521 cggggggcat ggcggtgccg agggcgtcgc ggaagcgggc cgcgtcctcg atcgcggccc
     2581 agcgttcctc accggcgatc cgcaggcgca gcgcgcggcg ggtgccctcc agctccgcca
     2641 gccagccggg cagcgcggcc gggtctgcgg cgcgggcggt cgcctccgcc acggtcaggt
     2701 cgccgagcag ccgcagcgcg tcgtgcagcc ggtccgggtc gccaacgcgg cgttcgggcg
     2761 tgagcagctg cagctccagc tccaggtcgg cgagggcggc cgggtcgatc aggtcgcgca
     2821 gctcctggag gccgagcagc tccgccagca gggtgcggtc cagtgacagc gcctgcgcgc
     2881 gccgctcggc cagcggggcg tcgccttcgt acatgaaggc ggccacgtaa ccgaactgca
     2941 ggctgctcgc gaacggcgac gggaacggcg tgtcgacctc caccacgcgg acctcgcggc
     3001 ggcccacggc ggccatcagc tcggcgaggc cgggcaggtc gaagacgtcc tggaggcatt
     3061 cgcgcatggc ctccagcacg atcgggaacg acccgtagcc ggcggcgacc tgcagcagcc
     3121 cggcgctgcg ctggcgctgc tgccacaacg gggtgcggcg gccggggcgg cggcggggca
     3181 gcagcagcgc gcgcgcggcg cattcccgga agcggctggc gaacagcgcc gagccgctga
     3241 cctgtccgac gaccagatcg tcgacctcct cgggttcgaa caggatcgag tcggacggtg
     3301 gcgcctcgtc cgcctcgggg aggcggacca cgatgccgtc gtcggagtac atcgtctgga
     3361 cctccaggcc gagacgctca cgcacgcgcg cctcgatcgc ctgggcccag ggggcgtgca
     3421 gcggcgcccc gaacggcgag tggacgcaga cccgccagtc gcccagctcg tcgcggaagc
     3481 gttccaccac gatggtgcgg tcgtcgggca ggaccccggt cgcctccagc tgctccccga
     3541 ggtaggcgag caggttgctg gcggcgttgt ggtccagccc ggcgccgctg agccgctcca
     3601 gccgcgtcgc cgggtcgagc gccaccagct cgcgcaggaa agcgccgagc gcgcggccca
     3661 gctccagcgg ccggccgggc gcgtcgccgt gccagaacgg cagcttgccc ggctggcccg
     3721 gcgcggggct gaccagcacc cggtcgtggg tgatgtcctc gatccgccag ctggaggcgc
     3781 cgagggtgaa gacctcgccg acgcgggact cgtagaccat ctcctcgtcc aactcgccga
     3841 cgcgggcctg gccctccccc accaggtaga cgccgaacag accacggtcg gggatggtgc
     3901 cgccggaggt caccgccagc cgctgggcgc ctggccggcc gctgagcacg tcggtgcccc
     3961 ggtcccagtt gagccggggg cgcagctcgg cgaactcgtc ggacgggtag cggccggaga
     4021 gcatgtccag gacgctctcc agcgcgctgc ggggcagctc cgcgtaggtt gccgcgcggc
     4081 gcagcagcgc ctccagccgg tcgagcggcc agtcgtccat cgccaccatg gccacgacct
     4141 gctgcgcgag cacgtcgagg gggttgcggg ggtagcgcat ggcctcgatc gccccggcgt
     4201 gcatccgctc caccaccacc gcgcattcga ccaggtcgcc gcggaacttc gggaacacca
     4261 tcccgcggct ggtggcgccg acctggtgcc ccgcccggcc gatccgctgc aggccctgcg
     4321 ccaccgaggt gggggcctcc acctggacga ccaggtcgac ggtacccatg tcgatgccga
     4381 gctccagtga gctggtcgcc accaccgccg ggaggcggcc cgccttgagg tcctcctcgg
     4441 tctggacgcg ctgctcgcgg ctgaccgagc cgtggtgggt gcgggcgatc tcggccccgg
     4501 cgaggtcgtt gaggctggcg cagagccgct ccgccagacg gcgcgagttg gcgaacacga
     4561 tggtggagcg gtgggcgcgg atcaggtcga gcagccgctc ctcgatgtgc ggccagattc
     4621 ccgtgcgttc cggcgccgcg ccctccacac cgtcggcgtc gtcagcgtcg ctgccccgcg
     4681 aaggttccgc gccgatcgcc gccaggtcct cgaccgggac caccactgag agatcgaacg
     4741 tcttctggct gggcggggcg accaccgtga ccggccggcc gccgctgagg aagcgggcca
     4801 cctcgtcgag ggggcggacc gtggcggaca ggccgatccg ctgcgccggg cgctccagca
     4861 actcgtcgag gcgttccagg ctgaccgcga ggtgcgcgcc gcgcttggtg gcgaccaccg
     4921 agtggacctc gtcgacgatc accgtgtcga ggtggcgcag cgcctccctg gcctgggagg
     4981 tgagcagcag gaacagcgac tcgggggtgg tgatcaggat gtcgggcggc gcctgggcga
     5041 agcgccgccg cgcatcggcg ggggtgtcgc cggtgcgcat ggcgacgctg agctgcggca
     5101 gcggcaggtc gagccgggcg gccgccgcgc gcagccccgc cagtggcgtg cgcaggttcc
     5161 gctcgatgtc ggcggccagc gccttcagcg gcgagacgta cagcacccgc aaccgctccc
     5221 gcgctgggac cggcggggcg gtcgcgagcc ggtcgatggc ggagaggaac gccgccagcg
     5281 tcttacccga cccggtgggc gccaccacca gggcgtcctg gccgccggcg atcgccgccc
     5341 aagcgcccgc ctgcgccgcg gtcggggcgg cgaaggcgtc ccggaaccac gcccgggtga
     5401 gcggcgagaa atgctccagg ggcgtgttcg gcgtgatcgg tgcgggtgtg gtgttcatag
     5461 ccacatgcta gatcgcggcc gtgacagccc gccgacggta tccacagccc agaacgcggc
     5521 attctgcggc cccatgtcgc gccgccgcct ctcggaacgc atggctgagg gtggcacact
     5581 gggccaaggg atctaattgc cgaggggtgg cgaacagaag atgggttggt tcgatcgacc
     5641 ggagttcgga cccaatatcg gtttgatcga cgaaatttac cgcaaatacc tcgacgatcc
     5701 gacctcggtc agcgaggcct ggcaggagtt cttcgccgag caggagcccg aggacaccga
     5761 cggcgacagc gacagcgacg gcgcggcccc ggcgacggtg gcgaccccaa cggcaccgcc
     5821 cgagccgcca ccggcgcgac ccgccaacgc tgcggccgca ccggccacgg cggaggctgc
     5881 caaggcggag ccgcccaagg ccggacccgc cgccgatggg gacggcgaca gcaacggggt
     5941 gcgccccgta ccgctgcggg gcgcggcagc gcggatcgtg gaggcgatgg aggccagccg
     6001 ggcggtgccg acggcgacct cgttccgggt ggtgccggcg cggctgctcg aggtgaaccg
     6061 caatgtcctc aacgggcacc tacgccgcca ccgcggcggc aaggtcagct tcacccacct
     6121 gatcgcctac gcggcggtca gggcgctcca ggccctaccg gcgatgacct cgatctacca
     6181 ggagcacgcc ggcaagccgc aggtgctgcg gcccgagcac gtgaacctcg gcctcgcggt
     6241 cgacacccgg cgcccggacg gctcccgcac cctgctggtc ccgaacatca agcaggccga
     6301 ccgcctcgac ttcgccagct tcctgcgcgc ctacgaggaa ctcatcgcca aggtccggac
     6361 caacaagctg gccgtggccg acttccaggg caccaccgtg acgatcacca acccgggcac
     6421 cgtcgggacg gtgatgtcgg tgccgcgtct gctggccggc caggcggcga tcatcggcgt
     6481 cggcgtgatc ggctacccgg ccgagtacct gggcgccgac ccggagacgc tggcggagct
     6541 cggcatcggc aagaccgtca cgctgacctc cacctacgac caccgcgtca tccagggcgc
     6601 cgagagcggc gagttcctgg ccaggatgca ccagctgctg ctcggctccg acggcttcta
     6661 cgaagaggtc ttccgcagca tgggcgtccc ctacgtgccg gtgcgctggc acgccgacgc
     6721 ccgcgccgag gacgacctgg aggccaccga gaagcaggcc cgcgtctacc agctgatcaa
     6781 catgtaccgg gtccgcggcc acctgatcgc cgacctcgac ccgctggagg tcaagccgcc
     6841 gtcgatgcac ccggagctgg acccggccag cttcgggctg accatctggg acctggagcg
     6901 ccgcttcctc accggcgggc tgtgccggga gcgggaactg ccgctcgccg agatcctcag
     6961 gatcctccgc gacgcctact gccgcaccgc cagcgtcgag tacatgcaca gctcccaggc
     7021 cgacgagaag gcctggatcc aggaacgggt cgaagtccca ccggcgaccc tgcccaagga
     7081 cgaccagctg cagatcctca acaagctgat cgaggcggag gtgttcgagc gcttcctcca
     7141 caacaagtac gtcggccaga agcgcttcag cctggagggc gcagagagcc tcatcccgat
     7201 gctggacgcg gtccttgacc gcgccgccga cgccgccatg gacggcgtgg tcgtcggcat
     7261 gagccaccgg ggccgcctca acgtcctcgc caacaccgtc ggcaagagct acggcgagat
     7321 cttccacgag ttcgaggggc acctggaccc cgccctggcg cagggctccg gcgacgtgaa
     7381 ataccacctc ggcgcggccg gcaagcacac cgcccgctcc ggccgccagg tgcgcatcga
     7441 ggtcgcctcc aaccccagcc acctcgaggc cgtcaacccg gtggccgaag gcctggtgcg
     7501 ggccaagcag gacctgctcg accggggcga ggaggcgccg gtgctgccgc tgctgctgca
     7561 cggtgacgcg gccttcgccg ggcagggggt ggtagccgag acgctgaacc tgtcgcagct
     7621 gcgcggctac cgcaccggcg ggacagtgca catcatcgtc aacaaccagc tcggattcac
     7681 caccagcccg gactacggcc gctcctcgac ctacgccacc gacgtggcca agatgatcca
     7741 ggcgccaatc ttacacgtga acggcgacga cccggaggcg tgcgtgcggg tcgcgcggct
     7801 ggcgttcgac taccgccaga gcttcaagaa ggacgtcgtc atcgacatgt ggtgctaccg
     7861 ccgcttcggc cacaacgagg gcgacgagcc ggcgttcacc cagccgctga tgtacgcgcg
     7921 catcaaggcg cggccaagcg tccgcaagct ctacaccgag gcgctggtca accgcggcga
     7981 cctgtcgttg gaggaggcgg agcgctcact ggaggcctac cgccagcgcc tccagcaggc
     8041 cttcgacgaa agccacgaac acgaccagga aggaggcggc ggggacgacg acggcgagga
     8101 cggcggttcg ctgccgggtg cgagctcggt cgagcggcgg acgggatcgc tggcaagcga
     8161 cccgccggtg gagaccgccg tcggacagga ccgcctccac cagatcgccg ccaagctcgc
     8221 ctcggtgccg gacgagttcc acgtccatcc gaagctcatc cgctggctcc gcgaccgctc
     8281 cgaggcgctc gggaagaacg ggggggtggt ggactggtcg ctggcggagg cgctggcgtt
     8341 cggctcgctg ctgctggacg gccgcatcgt gcggctgtcg gggcaggaca cccgccgtgg
     8401 caccttcagc cagcgccact cggtactggt cgaccaggag aacggcgagc agtacacgcc
     8461 gctcgccaac ctcgcgcccg accagggccg cttcttcgtc tacgacagcc tgctgagcga
     8521 gttcgccgcg ctcggcttcg aatacggcta cgcggtcgcc aaccccgacg cgctggtgct
     8581 gtgggaagcg cagtacggcg acttcgtcaa cggcgcccag accatcgtcg accagttcat
     8641 cgtcgccgcc gagcccaaat gggggcagcg ctgcggcctg gtgctgctgc tcccccacgg
     8701 ctacgaaggc cagggccccg agcactcaag ctcccgggtg gagcggttcc tgcagctcgc
     8761 cgcagacgac aacctccagg tggcggtgcc ctccacccca gcgcagtact tccacctgct
     8821 gcgccgccag gccctgcgcg ccacccgcaa gccactcgtg gtgctgaccc cgaagtcgct
     8881 gctgcgcctg cccgccgccc gctcgaccgc cgccgcattg agcgatggac gcttccacga
     8941 gatcctggcg gacccgacca accccgaccc gcaacaggtc cggcaggtgc tgctgtgcag
     9001 cggcaaggtc tcctacgacc tcgccaagcg gcgtgccgac cagggcgccg accaaggcgc
     9061 cggggcggtc gcgatcgtcc gcgtggaaca gctctacccg ctgccggccg agcagctccg
     9121 cgcccagctg gacgcctacc ccaacgccga ccgggtgctg tgggtccagg aggaacccga
     9181 gaacatgggc gcctggcggc atctgcgcct ccacgcccgg cgggcgcttg ggatcgagct
     9241 gaagggcgtc acccggccag cggcggcggc gccagccacc ggcagcccca gaatccacca
     9301 gcgcgagcaa caggaggttc tcgaccgggc ctttgcggaa taatccccga acgatcctga
     9361 tccgacgacc ccctcgacgt tccgacggcg gcccgcgacc atgagctgag gaggcgatcg
     9421 ccatgaaggt ccggcaggtg gcgagcagtg cggtggtgac ggtgggtcca atgcagtcgc
     9481 tgcgggaggc ggcgcaggtc atgtcgcggc accgggtcgg ctcggcggtc gtccaggatg
     9541 ccgagcagct gatcggcatc ctcaccgaac gagacgtgct caacgcggtc gccggcggcg
     9601 ccacgcccga ggacgtctcg gtgcaggaga tcatgaccgc cgacgtggtc accgtcgggc
     9661 ccgactggga cctgctggag gcggccggcg agatggccag gcggcgcatc cgccacctgg
     9721 tcgtctacga cggccgccag ctcctcgggg tgctctcggt ccgcgacgtg ctgcccgcga
     9781 tccttcccga cgtcagcacc agcgcggacg cccgaggcct gggatagcat ccgcccatca
     9841 gcgacgacgc ggaggtcacc gcgtatggat ctggacgagg tccttgccaa gatcgccgcc
     9901 gacgaggtcg agttcgtacg cttcgagcag accgacctgc acggcatctc ccgctcaaag
     9961 accatccccg cccgccacgt gggcgccttc gcccgcgacg ggctcaactt cctgctcggc
    10021 cagctcggcc tcgacgccca gggcggcgtg gcgccaggca ccggttacct ggaggaacgt
    10081 ggtttccccg actcccggct gttccccgac cccgagacct acgcggtgct gccctgggcg
    10141 gtggccaccg cccggctgct gtgcgacccg cacttcatcg acggcacccc cgcgccagcc
    10201 tcgccccgcc tggtcatgca acggctctgc caggacctgg ccgacgaggg cttcctgcac
    10261 ctctccggct acgagtacga gctgtacctg gtccgcgccg acgaccgcca gccgccgttt
    10321 cccggcatcc agatcttcgc gacgctgcgc aacaccttcg acgaggcctt cgtcagcgac
    10381 gtcctgcgcg ccatgccgcg cgtcggggtc gacatcatca ccgccaacgc cgagtacggc
    10441 ccaggccagt tggagatcaa cttcgcgccg gccatcggcg tcgccgccgc cgaccaggcg
    10501 ttcaccttca aaaacggcat caaggaaatc gcccgccgcc acggctacct ggcctcgttc
    10561 atgaccaagc cctacgccga ccagagcgcc tccggctgcc acctgcacga gagcctgctc
    10621 gaccgcgaaa ccggccgcaa cgccttcgcg gtggacgaca ccacccccgg caagctctcc
    10681 gacgtcggcc gctggtggat cggcgggcag ctcgcccacc tgcccgccct gaccgccttc
    10741 ttcgccccga cggtcaactg cggcaagcgc ttcaaaccgt ggagcttcgc accggtcaac
    10801 cggacgtggg gtcttgagaa ccgcaccgtc gcgatccgcg tcaaggggct cgcgggtgag
    10861 cgcctccacc tcgagaaccg ggtgccgtgt ggcgcctcca acccctactt ggtgggcgcc
    10921 gccgcgatcg ccgccgggct ggacggcatc cgcaaccgcg tcgagccgcc gcccgagacc
    10981 gacgggctcg cctatgggct ggagggcccg gccctgatcc cgacccggct ggaggccgcc
    11041 ctcgacgagc tggagaagga catggcgttg cggggcctcc tcggcgagga gctgatccgt
    11101 ttgttcctgg cggtcaagcg ccacgaggtc gccaaggccg gcgaccacgc ccgcgactat
    11161 gccgctggcg actgggccga ccgggtggac gagttcgagg tcgcggggct gttcgagttt
    11221 ctgtgaccgg cgacggtctg gctacggcac tgttgcgggg ggcggggtcg ctgcctggtg
    11281 agcgaccctg gcgaggaacg ggatgaggtt gtcgaggaac tcgttgttgg ggctggcggc
    11341 ggccgggtcg ttgtgcgcgt aggtggcctg gcggttgatc agggtcaggt cgcttgaggg
    11401 gatgctggac tgctgggcga ggatggcggc ggtgtccagg acgccctggc cgccgagggc
    11461 ggcgccgaag gcgtagatgg gcaggtgggc gccgaggtcg tggccgtgga tcgcgtcgac
    11521 gccgaggatg gcctgggcgg ggttggcgtt gccgtcggcg accgcgccgg cgtcgatggt
    11581 gagacgcagt gggtggtacc aggcgctgcc gtcgacgccg gccaggctgg ttccggagag
    11641 catgcgggcg tagcgctgga tgggggtgag ctcgccggcg cggacccagc cgcgcggcgt
    11701 cccgctggcg gcgaggtggc cggcgtggac ctgggcggcg accagtgccg gcggggaggt
    11761 ctcggtgtcc acgccgaagc cgaactgggc ctcgttggtg gttggcaccg gcggcctcag
    11821 gtcggcgggc agcagcggga agctctgtgc gagcgagagc ccgtgggggt cgatcaccgc
    11881 ggcggtggag ccggtcgcgg cgaacaggcc gaggaacggc gctgggatgc cgccgaacgc
    11941 cagccatggc gagctggcct ggaggttctg cagggcctgg gtggcctggt cggcggagat
    12001 gggggccggg ttgctgccgc cgtcgatgta gaccagaccg gagaggccgc gcgcgccggg
    12061 gctgccgttg aagtcccagg tggcgtaggc ggtggtgatc gtcccgccaa gggagtggcc
    12121 gccgagcacc actcggcggc cgtgctgacc ggcggcctcg accacgcggt ggaggtcctg
    12181 gatctcgacg ttcatgcccc agccgcgcgc gaacgggacc gccgcgtcgg gtatcggctg
    12241 gaaatgggtg gtgatgccgg ggtcgaccag ccagccgagg tagtagtcga acagctgctg
    12301 cggcgtagcc ttggcctgtt tggcctgggt gagcacggac tggtcttcga gctggttctc
    12361 gcggcgttcc accgaccaga cctgccagcg gcccggggtg cgccgcacga tgtcgcgcgc
    12421 gagcggcagg aagtagccgg cgcccgccga cgtgccgggg ctcaggacga gcacgttgcg
    12481 ggcgcggtcg gagccgactt tgaggacgcc gacacggttg aggctggcgg gggtggcggg
    12541 gtcgtcgaac ccttggagcc aggtgaagtg gatctcggtc gccgcgtcgg gccgcgccgt
    12601 ggtggtggtg gtggccgtgg cggtggcttg ggccagcgcc ggcgttggcg ctgcgaacgc
    12661 cgccaccacc aggcccaggg cgagggtgac tgcgagctgg cggaaccgct ccttgtggtg
    12721 gcgcatgcgc tgctcctcca ttcagcacct tcggcaccgt ggcgggcgcg gggtggccgg
    12781 cacccgccca cgggcggtgg cggcgggcgg cgggcggctc gcccctcgtt ggccgcccac
    12841 cgcccaccgc ctgcctccca ccgcgacgct attggccgcg tggggggcgg ttcaacgacg
    12901 cctggacgaa tttcgtgcgc cgtggctgtg cagctgcaca aggcgtgggg atcagggttg
    12961 tggccgccgc tccgatgtcg ccggcccgcc agggccgggg ccggggctgg gggtgcttgc
    13021 gacggccagg tagacaccgg cgaggtcccg tgcggtggtc aggtccaggc cggtgagccg
    13081 ctcgatgcgg cgcaggcggt aggcgagcgt gttgcggtgg acgtgcaggg cctcgctggt
    13141 ggcggcgcgg tcgtactggt gggcgacgaa ggcgcgcagg gtgcgcagca gctcctcgtg
    13201 atcgggagcg gccagcggcc agagcacccg ggcgcgcagc cgcgccgccg ggcccggcga
    13261 tcgggccacc agcagctcca cagggtgctc ctcggcccgg aggcggcccc gcaggccggc
    13321 ccgccaaccg tgctccgcca gcgccgccac gtcctcgcgg gcatcggcga gcgcggcgtc
    13381 gtcgcgcggc gccgcggacg ccgtcaccag cagcacgtcc ggcccttccc cgaggtcggc
    13441 gacgtcgagc gggcgggtgg cgctggtcag gccgacgacg cgggcgccgt cggtgaccgc
    13501 cagcttccag ccgcggcgcc gcagcctcgc ggccagcgcg gcgtggcgcc gcagcggctg
    13561 gccgggcagc agcaccacga acggcgcgta ggcctcctcc accgtcacgc ccagccgctc
    13621 ggccagctcg cggtcgccga ggtcgagcgg cgcggcgtcg ccgcgggtga gccgctccag
    13681 caggtcccgc gcctggcgct cctcctccga caggagcagg tcgcgttcgg cgaggtaggt
    13741 gtcggcgacc accgccgaga tccggtccac gtagcgcatc agcagcccgg ccgcgtccag
    13801 cagcgcgtcg tgctcgtcgg cgcgcgcgtg gcgccggatc agctcccaac ccacctgccc
    13861 gccgatcccg aacgcccgca ggaggtcttc cagccgtacc ccctcgccgg ccctggcgcg
    13921 ggcccaggcg cgcagcggcg cgaactcctc gtcgggcggc gccaccccca ggatcagcca
    13981 gcgccaccag cgcagcaggc tgcgccgcac gccctccagg acgtcgtcct gggaggcgcc
    14041 gggcagccgt tccagcgcgg cgatctccga ccggaacgcc gcggccatct ctctgctggc
    14101 gacgctcagc gggatcctgg atacgatcgc cgcgatccgc gtgtatgggc tggtctgtgg
    14161 cacctctggc ctccgctccg ccccgacagt ccgcgcgatc gcctcgagaa gctgggacgt
    14221 ggacgctgaa gaccacataa tgcttgatag cggcgtcgag ggtggggtgg cgatggcgag
    14281 acctgaaccg gtaccggcag tcggcagcgt gatgacgaac gcgaccgcgc aggaggacca
    14341 gcaggcgcat cagcggaccg ccgccgcgcg ggtcctggag ttggccgagg ccagcgggct
    14401 cgcgctggtg cggttcctct acgtcgacca cggcggcgtg gtgcgcggca aggcgagcgg
    14461 gcggcacggc cttggcgggc ggctgcgcag cggcatcggg ctgaccctgg cgatgcaggc
    14521 gatgaccatg ctcgacgagc tgcagccggt tgaggggacc gggccggtcg gcgagatccg
    14581 cctcgtcccc gatcccgagt cgttcgtggt gctgccgtac gcgcccggtg ccggggcgat
    14641 gctggccgac atggtgcggc tggacggcga gccgtgggcg gcgtgcccgc gcggcttcct
    14701 caagcaggcg gtggcggcgc tggccgccga gggctacgca ctgcaggccg ccttcgagcc
    14761 ggagttcacc ctgggacgcc gcgtcctggc ccccgacgca ccagcggcca gcggcggtgc
    14821 tccggggctg gagcggctgg agccgctcga ccggagcctt tgcttctcga ccaccggctt
    14881 cgatgcggcg cacggctacg tgatcgagct ggtgcgggcg ctggaggcgc aggggcttgt
    14941 cgtggagcag tactacccgg agctcggctg gggccagcag gagctgtcga tcggctacgc
    15001 gcccgcgctg cgcgccgccg acaaccaggt cctgtaccgg gacaccgccc gtggcgtggc
    15061 ggcgcggcag gggctgtggg cgacgttcgc gccgaagccc atccccgacc aggcaggcaa
    15121 cggctgccac ctgcacgcct cgctgtggga cgccgcgctg cggcgcaacc tcttccacga
    15181 cgcgagtgac cgctacggcc tgtcggagct tgcctaccac ttcgtcggcg ggctgctcgc
    15241 ccacctgccc gcgctggtcg ccctcacctg cggcagcgtg aacagctacc ggcgccttgg
    15301 cccgcggtcc tggtcgagcg cctaccgctg ctacggcccg gacaaccgcg aggcggcggt
    15361 gcggatcgcc tcgcccatgt ggggcgccga ggaggcgtcg accaacctgg agctgaagtg
    15421 ctccgacgcc accggcaacc cctacctcgc gctgggtgcc tacatccatg ccggcctcga
    15481 cggcatccgc aacaagctcg acccgggcga ggcggccgac gccgacccgg acacgctgcc
    15541 ggcggcggtg cggcgacggc gtggcatcga ccgcctgccc gactccctgc cggcggccct
    15601 ggacgccctg gcggccgaca agctgctgat ggaggcgctc gggccgctcc gccgcgacgc
    15661 ctacctgcgc gtcaagcgcg ccgaggcagc cgccttcgcc gccggggacg ccgcgttcga
    15721 gtgctaccac cacttcatga agttctagcc gcaccagtgc caagtgccag ggggatggaa
    15781 ccgggaaggg ggaacggacg atggcgacgg tggagccggt tgccggcggc gagctgctct
    15841 ccgagcgggt cgcgggcggc atcctgccga aggtactcaa ctcgttcgac atggtggcga
    15901 tctttctcgc catcgtgctg ttcatcacca acgccgcggt gatccagtcg gccgggccgg
    15961 ccgcgttcgg ctggtggatc ctcgggttcg tcgccttcct gatccctggt gcgatcgtca
    16021 ctgggcagct cgggttgatg ttccctggcg agggctcgat ctacctgtgg acccacaagg
    16081 ccttcggcgc cttctggggg ttctttgccg ggttctgcgc ctggtggccg ggcgtgctgg
    16141 tgatggtggc caccggcgtc ggcacgctgg cgttcctcgg gttcgtgttc cccggcacgg
    16201 tcggcgcctg gagcgtcaag acccagggta cggcgatcat cgggttcatc gtgctgtcgg
    16261 ccctgctggc ggtgcagcgc ttccgggtca cccagaacat cgtcaacgtc gtggtggtcc
    16321 tctacggcct ggcgatcctg ctgatgctgc tggccgggat cgtgcacctg gccggcgggc
    16381 acgccgccga agtcagcccg gcgcagctgt cgacctgggc gcccggcagc aagagcggca
    16441 tcaacttcac caactggacc ttcttcggcc tggtgatcct cgcgctgctc ggcgtggagg
    16501 tgccgctcaa catgggggtg gagatccgcg aggtccgatc gatcacccgc tacctgttct
    16561 ggggctcgat cggggtgatg gtggcctacc tggtcgccac ctgggcggtg atggtcacgg
    16621 tcccggcggc gtcgggccag tcggcggcgc cggtggcggc ggcggcggtg aagatctcgc
    16681 tcggcacctt ctggggcgat ctggtggggc tgatcctggc gggcttcttc ctcttcatca
    16741 ccgtggtcta caactactcg ttcgcgcggc tgatctttgt ctccgggctg gaccggcgcc
    16801 tgccgcgcgc gctgagccgc gtcaacgccg ccaaggtgcc ggacgtggcg gtctggaccc
    16861 agtcgtgtat cgccgcgctg ttcaccctgg tcgccttcgt gatcctgccg gtcgcctcgg
    16921 gcggcgccaa gcaggtcgac gtccagacca aggtctacga catcctgcag gcggcggtga
    16981 cggtaatctg gtgcgtgtcg atggtggtgc tgttcatcga cgtgctggtg atcgtgcgcc
    17041 gctatgccgg cgtgttcgcg gcgcggcggc tcggcggcgc cgccaacgcg ggggtgttct
    17101 gggtctgctc ggtgatcggc gcgctggcct ccctgatcgg catcgtggcc acactcagcg
    17161 gctcgtggac gccgctgatc tccaacgatg ccggcaccgt ctcggtgttc ggcgcgcaga
    17221 tcacctacgg cgactggttc tactgggtcg gcggcatcgc gctcgcctcg atggtggtcg
    17281 gggcgctgct ctacctgctc gggcgccgca ccgccgcgtc ggcggcacgg tcgggctccg
    17341 ggtctgggac gacgctgccg gcgtcagagg cgggaggctg acccggggcc ggcccccggc
    17401 aagggcgtcg ggcgtcaggt cgtgccgagc atctggtaga gccggaaagc gccgcccgcc
    17461 gccgcgccct ggacccgctg cagcgtgcac cagtacaccg cttcggcgcc ggggacgtcg
    17521 cgcagcaact ggccatcgtc ggtcaccagg cgggcgccgg agcggccaag gaccgcgacc
    17581 accatccagc gtccgctgga gacctggtag gcgtggagca cgccgaccgg cagggtgagg
    17641 tcgacccagc gcttatgggc ctgcagcaac gcgtcgatct cgtgccggtc gcggcgcagg
    17701 ttcggggagt tgggggtata gatcgcggcg agcatggcgg cgtcgcggcg cagcagggcc
    17761 acctgccgga tctgcatgag gtggcggaag cgctgcacaa cagcggcctg cagcgtacgc
    17821 atggcctggt cgcccgagga cggcggcgcg ctggccggcg gcgtggcggt ggtggtggcg
    17881 gtccccttgg tgatgagcac gccaagggcg ccaccgcaga acagcagcac cagcaccagc
    17941 ccgatgaacg cgagcccgat caggaccccg cggtcgcgcg gccctgccgg tccggtgctg
    18001 cgctgcggca accgcagccc gcgacggcgg agggtcaggg cggcatcccc tcacttccag
    18061 tcccgccgct gccgccgcgg cgggcctcgg tacgctcctc ggcggcaagg cggccaaggc
    18121 cggagtcgcc gccgggcccg gcgtcctcct cgtcgtggac cacgaagcgc atcgccgcgg
    18181 cgaggccgtg atggcctgcc cgctcggcga tcagccggtg catccgctgc gccaccagcc
    18241 gataggcggg gtggccctgt ggctcggagc gcagctccgc caggtgcagc agctcccggg
    18301 cgttcagggt gatggcgaag cgaacccggt gggcgaggct gaccgcatag ggcgcttgtg
    18361 ccgggaaccg tggttccagc gcacggtaca gcgaagcaga agcgcgcatc acggcagcga
    18421 accggtcccc gagctcggcg gcctccacgt cggcggggac gtcgtagccg aggtccgggc
    18481 gcagcggctg ccagacgatc gtgcacatgc ggtggcgctg caggtcgcgg aaggcgccgt
    18541 agtcggcgac cacgtcgaag gtgtagaggg cgcgctcgaa cgcccgcccg ggccggtgcc
    18601 gccggttgcc gcgccgcccg acgtaggcgg ccagcagccg cgcgcgctcc tcggtggtga
    18661 gccgtgcgac gcgggcggcg accgcctcct cccccacccg caggtgcggg taggcgatcg
    18721 cggtgaggat cttgtcctcg gcggccgggt cgaactcgac caggcgcacc gacggcgcct
    18781 ggtcgcccac gccgcggtcg gggtcaccga gcagcgcgtc gaccaccttg gcggtctcct
    18841 cggcggtctc ggccaggtag cgggaccagg caacgccgcg gtcaggacgg tcgacccggg
    18901 tgaggaacac cgggatcacc tggcggagct cccgcagcag cagttccgcg gcggcgcggg
    18961 cctccggcag ggggtgggcg cgcagacgca gcagcagcgc ctcgtaggac tgggcgctgg
    19021 cgtagatgcc gacgttcgag gtggtcgcgg cgggcagcag gccccgcagc ccgtccaggg
    19081 cacgcgcctt gaccgcgcgg cgccaagccg gctcgctggc ggccggatcg cgcggcagcc
    19141 gctccgccag aacggcgatc agcgccggca ccagctcgct gtaggcggtg aagagcccgt
    19201 cgagcactcg ccggtactcc gggccgagcg ccgggtcggc gtcgacgtcg gggtccaggt
    19261 ggtagcgcca gcgaccgccg ggccggtcgg tgtaggagac gtagcgggtg gactgctcca
    19321 ggtaggcggc cagccggccc cactccagcg ccttggtgag caggttcgag gcctgctcgc
    19381 aggcgacgtg ggcgccgccg agctgggcca ccgagtcgtc cccgtactgg tccaggacgc
    19441 gatggtacag cgcggcggcg cgcgcctccc ccaccgggcc gctcgttggc ggcgtcgtgg
    19501 gcagctcgcc ggcgaactcg tccaggaaca gccgccgcag gctcttgccg ctgcgggagt
    19561 agcgcgagaa cagcgccccc ttgaccgcct cggggaggtt ccgcagtgcg aacaccggcc
    19621 ggtcggtgtt ggtgaaccag ggggcgagga tggcccgctc ctcctcggtg aacggctcga
    19681 cccaccagga cggcgctgcg gtcatgcgag ccccagcagc ggttcgaggc cgatcgtgag
    19741 gccgggccgg gccgcgaccg cgcggaccgc cagcagcacg ccgggcatga acgagacgcg
    19801 gtccagggag tcgtggcgga cggtgagcag ctcgccctgg ccaccgaaga tgacctcctg
    19861 gtgggcgacc aggccgggga ggcggaccga gtgcacccgc actccgtcga cctcgccgcc
    19921 gcgggcgccg ctgacggact cgcggctcgg tggcggctgg cgctggtccg gtccctgccg
    19981 cgcggcggcg atcgcctggg cggtgcggat cgcggtgccg gagggggcgt cggccttgcc
    20041 ggcgtggtgc agttcgatga cctccgccgc cgggtagtgg cgcgccgcga tggtggcgaa
    20101 ataggtcgcc agcaccgcgc cgagggcgaa gttcggtgcc cacaccgcgt tgccggccga
    20161 cgggccggcg aggctgcgca gctcctccat ggcgccgtcc gccagaccgg tggtgccgga
    20221 caccagctgc accccctcgg gcaggagcgc cctggccgcg gccatggtcg cggcgggggt
    20281 ggagaagtcg accaccacct cggccttggc cgccagcagg tcggcgaccg cgccgcccac
    20341 gaccaggccg ccgggcgcct cagcgaccag tgctcgcagg tcttgcccgc tcccccgctg
    20401 gcccgcgacg gcggcgacca gctcgagatc gtcagtggcc gcgaccgcgc ggcaggtcgc
    20461 ctgccccatc cgcccggtcg ctccgaggac tccgatgcgc atctgccctc ccatggtggg
    20521 gtcgtaccgc cgatcgcgag cctgggggct ggctcgactc cgggattctg gccgacgtcg
    20581 ttgacggccg acggcgtgtc aggataccgc gttgcgtcca tcctggcgga atgcgacggc
    20641 ctgacgcggc cagaggaccc gtttcatggc tttgcgaact caggcagggc gctggtccgc
    20701 cgcctatcgc atgcgaccag cccgaggcca tagcggttgg cggctgctgg cggctacgct
    20761 ggcactatgc tcgcgtcggt agacaacgaa gcggtcgtca tggcggtgct cgccgaccgc
    20821 ttccggttgc cgatggaccg gctcgtgccg ggcgaggtcg ccatcgccga ggtcgaacac
    20881 cccagcctgc cgatccgcgc cttcagccgc gagggcggcg cggtgctcgc ggtcgccccg
    20941 cgccacgcgc cgctgctgcg ccgctacctg cacgacccgg ccacgctgct ccccgccttg
    21001 gaggcggtcg ccgcacgcct ccccgggcat ctgttctccg gcgtcgcccg caccggcgtg
    21061 gccatccccc cgcccgctgc cgaggtcacc gtcctcgacg ccaccgaccc ccgcctcccc
    21121 gactgggtcc acggccactt caccggcgag gcctgggcgg tcctggacga ccacagcgag
    21181 gtgctgtcga ccgcggtcct caaacgctac gacgaccgcc tccgcgagat ctccgtcggc
    21241 accaccgaag ccgcccgcgg ccggggcctc gcccgcgcgg tggtcgccgc cgccgcccgc
    21301 gccgtcctca gcgaaggccg cgcggtcctc tacaaccatg cccctgacaa ctacgcctcc
    21361 gcccgcgtcg ccgaatccgt cggcctccac gaattcggcc gctaccacgc catcgtcccc
    21421 gaccgccgcc tcggcgccga gtacggcgag gacgacacca ccgccgaggc cgacaaccgc
    21481 tgagggccgg ctcttatcag ttcagcgcgt cacggtgacc gctggcgttt ccaacttccc
    21541 agcttgcggg cgatcttgtc gagttcggga tgtgcggtca ccacgcccac gacgaactcg
    21601 actgcctcag cggtcgatgg tggttcgacg tcgtagccgt tcagtccgta gaacaccaca
    21661 gtggcagacc aggccagccg cttattgcca tcaacgagcg cgtgggctcg tgcgaccccc
    21721 tcaagcagcg c
//
LOCUS       DASWDC010000042         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1178383, whole genome shotgun sequence.
ACCESSION   DASWDC010000042 DASWDC010000000
VERSION     DASWDC010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178383"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VF995_01255"
     CDS             <1..1320
                     /locus_tag="VF995_01255"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9342229.1"
                     /translation="LPDLPEPLLAGYLDAALAALRAASAGELPGALRQYQTWTPRRLK
                     HPRVLGLVKRALGTDAAFRAAVDGRVLEEEEALARLLRAGRHVEALASGEAPEAVAKV
                     GLALGAAGALAVQAASDALATSSAQAEAEAARSALAGATGELDAVRVRAEAEAAAGRA
                     AREELRLVRRELRRSERQVEALTERCRELEAEVRRAEAALQAARQQAADEQRRQSGRI
                     AELQAHLAETQRQYRTLRRASVQVDPMLAEAVGALERDLATLRRAAGLPDPGRKPPGS
                     PVVAGRARQPERRTPLPVPGGRGADDPETLAAWMSVPEVLVLVDGYNVTKHERGFPEK
                     SLEDQRTLLVDLCRRMSRRWGAHVTVVFDGANVAPVPTRLPLGAVDVVFTDPGRTADD
                     EIVARVNAAPPERPVVVVSSDNELRQRSAALGASVTRSTALLGLASR"
     gene            complement(1349..1615)
                     /locus_tag="VF995_01260"
     CDS             complement(1349..1615)
                     /locus_tag="VF995_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342230.1"
                     /translation="MAHEEPLRISCDGCPDGCKDCLVGFFFAERDAEVLRLGAVGADA
                     TPALPATVGPGTLPPDLARVLGALSAAGLEPQVLDLRRRRRRAS"
     gene            1898..>2273
                     /locus_tag="VF995_01265"
     CDS             1898..>2273
                     /locus_tag="VF995_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342231.1"
                     /translation="MPVRQRARKSWKVRTPLLLILAVAALSGAGLAIRLPATATPASQ
                     VDLERLGTQIDVLDEQLNQAIIQLGPLNQQLAMAKQSVRQQQVQLDQIQRLIGAQAAT
                     EYKGGGVDLVAALFAGSDSKDLV"
BASE COUNT          305 a          856 c          839 g          273 t
ORIGIN      
        1 ctgccggacc tgccggagcc cctgctcgcc ggttacctgg acgccgccct ggcggcgctg
       61 cgggcggcga gcgccgggga gctgcccggg gcgctgcgcc agtatcagac ctggacgccc
      121 cgccgcctca agcacccgcg cgtgctcggc ctggtcaagc gggcgctggg gaccgacgcg
      181 gccttccggg cggcggtgga cggccgcgtc ctggaagagg aggaggcgct cgcccggctg
      241 ctgcgcgccg gccgccacgt cgaggcgctc gccagcggcg aggcgccgga ggcggtggcc
      301 aaggtcggcc tcgccctggg cgcggcgggc gcgctcgccg tccaggccgc cagcgacgcg
      361 ctggccacca gcagcgccca ggccgaagcg gaggccgccc gctcggctct ggccggagcc
      421 accggggagc tggacgcggt ccgggtccgg gccgaggccg aggccgccgc gggacgggcc
      481 gcgcgagagg agctgcggct ggtccgccgc gagctgcgcc gcagcgaacg gcaggtcgag
      541 gcgctcaccg agcgctgccg cgagctggaa gcggaggtgc gccgggccga ggccgccctg
      601 caggccgcca ggcagcaggc cgccgacgag cagcggcgcc agagcggccg catcgcggag
      661 ctgcaggcgc acctggccga gacccagcgt caataccgca cgctccggcg cgcctcggtg
      721 caggtcgacc cgatgctcgc cgaggcggtc ggcgctctcg aacgggacct cgcgaccctg
      781 cgccgcgcgg cgggccttcc cgaccccggc cgcaagccgc cgggcagccc cgtggtcgcc
      841 ggccgggcgc gccagccgga acgccgcacc ccgctgccgg tccccggcgg ccgcggcgcc
      901 gacgaccccg agaccctggc ggcctggatg tcggtgccgg aggtgctggt gctggtcgac
      961 ggctacaacg tcaccaagca cgagcgcggc ttccccgaga agagcctgga ggaccagcgc
     1021 accctgctgg tcgatctgtg ccggcgaatg tcgcggcgct ggggcgcgca cgtcaccgtg
     1081 gtcttcgacg gcgccaatgt cgccccggtg ccgaccaggc tgccgctcgg cgcggtggac
     1141 gtggtgttca ccgaccccgg ccgcaccgcc gacgacgaga tcgtcgcccg ggtcaacgcg
     1201 gccccgcccg agcggccggt ggtggtggtc tcctccgaca acgagctgcg ccagcgctcg
     1261 gcggcgctcg gcgccagcgt cacccgctcg accgccctgc tcgggctcgc cagccgctag
     1321 gaagcgccgc gcggcggctc ggagaggcct aggaggcgcg gcggcgccgg cggcggaggt
     1381 cgagcacctg cggttccagg ccggcggcgc tcagcgcgcc aagcacccgg gccaggtcgg
     1441 gcgggagcgt ccctgggccg acggtcgcgg gcagcgctgg ggtggcgtcg gcgcccacgg
     1501 cgcccaggcg gaggacctcg gcgtcgcgct cggcgaagaa gaacccgacc aggcaatcct
     1561 tgcagccgtc ggggcagccg tcacaactga tccgcagggg ctcttcgtgc gccatgtcag
     1621 gtcctctcga ttcgccggtg cctcatgatt ttgtggtagt tcgaccttca acgccatgac
     1681 cgtatcgggg gggtgtgaca aaacaacggg cttcggggcg tcgctcgcga aagttttccg
     1741 ccgaaagggc gattcgacag acttgccttc acgttatcga gtcgttacgg ttccgccagc
     1801 accgtcagcg ccccccagcg ctccgtatcc ccccggatag ggcggtgcct ccccccggtc
     1861 cacttgcttc accgcactga agggagaacg acgccacgtg cccgttcgcc agcgggctcg
     1921 gaagtcgtgg aaggttcgga cgccgctgct cctgatcctt gcggtcgccg ctttgagcgg
     1981 cgccggcctg gccatcagac ttcccgccac cgccaccccc gcctcccagg tcgacctgga
     2041 acgcctcggc acccagatcg acgtgctcga cgagcagctc aaccaggcga tcatccagct
     2101 cggcccgctg aaccagcagc tcgccatggc caagcagtcg gtcaggcagc agcaggtgca
     2161 gctcgaccag atccagcgcc tcatcggcgc gcaggccgcc accgaataca agggcggcgg
     2221 ggtcgacctg gtcgcggcac tgttcgccgg cagcgactcc aaggacctgg tcg
//
LOCUS       DASWDC010000043         3760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1178420, whole genome shotgun sequence.
ACCESSION   DASWDC010000043 DASWDC010000000
VERSION     DASWDC010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178420"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VF995_01270"
     CDS             complement(<1..432)
                     /locus_tag="VF995_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9342232.1"
                     /translation="MDALDCILTRRSGGRLTEPAPSGGNLATLLAAAAAAPDHGLLRP
                     WRFIVLRGHALHRLGEVFAKAEEARGAEAGTPVREDALARTRGKPLRAPLIVALIASP
                     RPSPKVPAWEQLASAAAAAQNLCLAAHTLGYGSMWRTGWYGD"
     gene            complement(590..1519)
                     /locus_tag="VF995_01275"
     CDS             complement(590..1519)
                     /locus_tag="VF995_01275"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX9342233.1"
                     /translation="MATARPGPERPRMVTPEAVALDFRTANVGSRLLAYALDILVIGV
                     VIVAVVLMVVLSGTTLPQWLAVASAAILIPAWYFGYFIALETLWRGRTLGKAALGLRV
                     VTREGGPVRFRHAAVRALLGLVDFVASGGFLAVAFILLGRDNQRLGDIVAGTLVLRER
                     SALPAPAPASFEPPSGLRSYTVTLDVSGLANEEYQTLRSFLLRATSLRPAPRAALAQQ
                     LATPLAARLRPPPPAGVSPETYLACVAAAVQQRQRLAAGPARPLPVPAAPQPGAPAPI
                     PAGPLPPADEQTDEQTGDQTGEQGDGGAFTPPA"
     gene            1587..2633
                     /locus_tag="VF995_01280"
     CDS             1587..2633
                     /locus_tag="VF995_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017559250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX9342234.1"
                     /translation="MDIDRFIDANSQLWDRLAQLVGRAERGVGRLDGAELDELVRLYQ
                     RAATQLSYARTEFRDPALVAKLSRLVARAGAVVYGSRPRTLRSFGRFFAVTFPAAVWS
                     ARWFVLVATLVSLLPAAVFGIWLASSPAAIEAAAPAAVREAYVNHAFEDYYPSKPAVD
                     FAAHVFSNNVVVAFEAFALGILGCVGTFYVLLLNGANLGVAAGLFAAVGQQAKFWGLI
                     LPHGMLELTSVFIAGGAGLRLGWTLIDPGDRRRAEALAEEGRRAVVIVLGLVLSFAVA
                     GSIEGFVTGRPWPTALRVGIGALAEAAFLTYVTVCGRRAAAQGLTGALGEEDGGGWLS
                     TGQDQSRPVALTLR"
     gene            complement(2600..>3760)
                     /locus_tag="VF995_01285"
     CDS             complement(2600..>3760)
                     /locus_tag="VF995_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9342235.1"
                     /translation="PAIVPLGAEARITWTIAAQARAVHGGGRGARPRPARVRLADELA
                     PSLRAATRRARLTVPARGRASAATTIRPSRRGRFTPNELVLRVEGPLGLMARQGRRSL
                     PGTLRVYPPFHSRDEAELRINKARILEVGLRSAQGRGGGTEFDSLREYGVDDEFRRMD
                     WAATARAGKPIVRTYRAERNQTVLLLLDCGRTMAGRVAGVPRLDHAMDAVMMLTAVAT
                     RLGDRAGLVAFDAEVRAMVGPGHARDQLARVTEAMYSLEPALAESDYRGAFATTLARF
                     RRRTMLVVFTELAEQAVAETLLPALPLIARDHLVVVAGVTDPEVHAWALATPAEAGTT
                     YRKAAAVAALADRRRTVARLRGLGAVVVDALPGRLAPELADAYLRVKATGRL"
BASE COUNT          461 a         1337 c         1451 g          511 t
ORIGIN      
        1 gtcgccgtac cagccggtgc gccacatgct gccgtagccg agggtatggg cggcgaggca
       61 gaggttctgg gccgcggcgg cggcggaggc gagctgctcc caggcgggga ccttggggct
      121 cggccggggg ctggcgatca gggcgacgat caggggggcg cgcaggggct tgccgcgggt
      181 gcgggcaagg gcgtcctcgc ggacgggggt gccggcttcg gcgccgcggg cctcctcggc
      241 tttggcgaag acctcgccga ggcggtggag agcgtggccc cggagcacga tgaagcgcca
      301 ggggcgcagc aggccgtggt cgggggcggc ggctgccgcc gcgagcaggg tggcgagatt
      361 cccgccgctg ggggccggct cggtcagccg gccgccgctg cgccgggtga ggatgcagtc
      421 aagggcgtcc ataggtcgcg tggtcttcct gacgctgccg attcctggag tttgaggttg
      481 cagcttaggg ttgcctaata cagtagcgga tctcgcgcgg tgcgcggttg tcgcggttgt
      541 ctgggcgccg cgagtgacga gtcggtcctg ttgaggggtg ccgctgacgt cacgccggtg
      601 gcgtgaacgc tccgccgtcg ccctgctcac cggtctggtc accggtctgc tcatcggtct
      661 gctcatcggc tggaggcagc ggcccagccg gaatcggcgc gggcgccccc ggttgcggtg
      721 ctgccggcac cggcagtggc cgcgccggcc cggcggcgag gcgctgacgc tgctggacgg
      781 cggcggcgac gcaggcgagg taggtctccg gcgacacgcc agctggaggc ggcgggcgca
      841 ggcgggcggc gagtggggtg gcgagctgct gggcgagggc tgcccggggg gccgggcgca
      901 ggctcgtggc gcggagcagg aacgaacgga gcgtctggta ctcctcgttg gcgaggccgg
      961 agacatccag ggtgacggtg taggaccgca ggccggacgg gggctcgaag ctggccggcg
     1021 cgggggcggg cagggcgctg cgctcgcgca ggaccagggt gcccgcgacg atgtcgccga
     1081 ggcgctggtt gtcgcggccc agcaggatga aggccaccgc gagaaacccc ccgctggcga
     1141 cgaagtcgac cagcccgagc agcgctcgca cggccgcatg gcggaaacgg accgggccgc
     1201 cctcccgggt caccacccgc aggccgaggg cggccttgcc gagggtgcgg ccgcgccaca
     1261 gcgtctccaa ggcgatgaaa tagccgaaat accaggctgg gatcaggatc gccgcggacg
     1321 cgaccgccag ccactgcggc agggtcgtgc cgctcaacac caccatcagc accacagcga
     1381 cgatcaccac gccgatgacc agtatgtcga gcgcgtaggc gagcaggcgc gaaccgacgt
     1441 tcgcggttcg gaagtcgagc gccaccgcct ccggcgtcac catccggggc cgctcgggac
     1501 caggtcgcgc ggtcgccatg atcagtgagg ttccttctgt tgcccgcggg ttcgtcgccc
     1561 tgactttatc cccgtaggat tcaccagtgg acatcgaccg cttcatcgac gcgaacagcc
     1621 agctctggga ccggctcgcc cagctcgtcg gccgggcgga gcggggcgtc gggcgcctcg
     1681 acggcgctga gctggacgag ctggtgcgcc tctaccagcg ggccgccacg cagctgtcct
     1741 acgcccggac cgagttccgc gacccggcgc tcgtcgccaa gctgagccgc ctggtggccc
     1801 gggccggcgc ggtggtgtat ggcagccggc cgcggacgct gcggtcgttc ggccgcttct
     1861 tcgcggtcac cttcccggcg gcggtctggt cggcgcgctg gttcgtgctg gtggcgaccc
     1921 tggtgagcct gctgcccgcc gctgtgttcg gcatctggct ggccagctcg cccgcggcga
     1981 tcgaggcggc cgcgccggcg gcggtccgag aggcctacgt caatcacgcc ttcgaggact
     2041 actacccctc caagccggcg gtcgacttcg ccgcccacgt cttctccaac aacgtggtgg
     2101 tcgccttcga ggcgttcgcg ctcggcatcc tcggctgcgt cggcaccttc tacgtgctgc
     2161 tgctgaacgg cgccaatctc ggcgtcgcgg ccggcctgtt cgccgcggtc ggccagcagg
     2221 ccaagttctg gggtctcatc ctgccccacg ggatgctgga gctgaccagc gtgttcatcg
     2281 ccgggggggc gggcctgcgg cttggctgga cgctgatcga cccgggcgac cgccgccgcg
     2341 ccgaggccct cgccgaggag ggccgccgcg cggtggtgat cgtgctcggc ctggtgctca
     2401 gcttcgcggt cgccggctcc atcgaggggt tcgtcaccgg gcgtccctgg ccgaccgcgc
     2461 tccgggtcgg gatcggtgcg ctcgccgagg cggccttcct cacctatgtc accgtctgcg
     2521 gccgccgcgc cgccgcccag ggcctgaccg gcgccttggg cgaagaggat ggcggcggct
     2581 ggctcagcac cggccaggat cagagccggc cggtcgcctt gaccctgagg taggcgtcgg
     2641 ccagctccgg cgcgagccgg ccggggagcg cgtcgaccac caccgcgccg aggccgcgca
     2701 gccgcgccac ggtgcggcgg cgatcggcca gcgccgcgac cgccgccgcc ttgcgatagg
     2761 tggtgcccgc ctcggcgggg gtggccagcg cccaggcgtg cacctccggg tcggtcacac
     2821 cggcgacgac caccaggtgg tcgcgggcga tcagcgggag ggccggcagc agcgtctcgg
     2881 cgaccgcctg ctcggccagc tccgtgaaca ccaccagcat ggtccggcgg cggaagcgcg
     2941 ccagggtggt ggcgaacgcg ccccggtagt cgctctcggc cagcgccggc tccagcgagt
     3001 acatcgcctc ggtgacccgg gcgagctggt cgcgggcatg ccccggcccg accatcgctc
     3061 gcacctcggc gtcgaaggcg accagcccgg cgcggtcgcc gagccgggtc gcgaccgcgg
     3121 tcagcatcat caccgcgtcc atggcatggt cgaggcgagg cacgcccgcg acccgcccgg
     3181 ccatggtccg cccgcagtcc agcagcagca gcacggtctg gttccgctcg gcccggtagg
     3241 tccgcacgat cggtttgccg gcgcgggcgg tcgccgccca gtccatcctg cggaactcgt
     3301 cgtcgacgcc gtactcgcgc agcgagtcga attcggtccc gccgccgcgc ccctgcgcgg
     3361 aacgcaagcc cacctccagg atgcgcgcct tgttgatgcg cagctcggcc tcgtcccggg
     3421 aatggaacgg cgggtagacc cgcagggtgc cggggaggct tctgcggccc tggcgggcca
     3481 tcagcccaag cggcccctcc acgcgcagga ccaactcgtt tggggtgaag cgcccacggc
     3541 gggacggccg gatggtggtc gccgcgctcg ccctccctcg tgccggcacg gtgagcctgg
     3601 cccgccgggt ggcggcccgc agcgacggcg ccagctcatc ggcgagccgt acccgggccg
     3661 gccggggccg ggcgccacgc ccgccaccat gaaccgcccg ggcctgggcg gcgatcgtcc
     3721 aggtgatccg cgcctcggcg ccgagcggca cgatcgccgg
//
LOCUS       DASWDC010000044         6525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1090427, whole genome shotgun sequence.
ACCESSION   DASWDC010000044 DASWDC010000000
VERSION     DASWDC010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090427"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VF995_01290"
     CDS             <1..258
                     /locus_tag="VF995_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9342236.1"
                     /translation="SRAEHPTLVLLDIMMPRLDGLEVCRRLKAGPETSDIVIVMLTAQ
                     AQERDRDHGLAAGADDYFTKPFSPLALLNMVENLRVRQNAG"
     gene            complement(284..715)
                     /locus_tag="VF995_01295"
     CDS             complement(284..715)
                     /locus_tag="VF995_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX9342237.1"
                     /translation="MTMPPAALLLFRLGGRGYASPRERVLGIVDVGAQPPVPGLPSST
                     LAALDGNGGLLPLVSLRVALGLPDLGAEGRILVVSTNHGPTGFLVDEVVQVLEPDPSA
                     HPIRVRENRLATGYVTGTVDALGATWLLVNWDAIRVPSRSA"
     gene            939..1475
                     /locus_tag="VF995_01300"
     CDS             939..1475
                     /locus_tag="VF995_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX9342238.1"
                     /translation="MFESREIPGVWLIRLQPVGDVRGRFIETYRTSWLPFAKPMVQGN
                     RSDSVPRVLRGLHYHRRQADLWFVQAGRVTTVLVDTRIGSPTEGKHTTVQMGGGEDLA
                     VYIPEGIAHGFYAHDHVVMTYLVTGEYDGSDELGIAWNDPDLGITWPDQDPILSERDR
                     QNVRLADVPADRRVPYAG"
     gene            1602..3569
                     /gene="ftsH"
                     /locus_tag="VF995_01305"
     CDS             1602..3569
                     /gene="ftsH"
                     /locus_tag="VF995_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX9342239.1"
                     /translation="MLEHPEPHPHPTEHLMSNTAKPGPPPERPPRPAPPPPPKWRGWL
                     PIIGLLLTMLILFLPGRMSQSQVKTLNYQPDLKDKITANQVKSIQLGTDGHIDGVLAD
                     GTHFRSQYPVNDTSFYTLLNDHNVTVVKAVGSQTSLLDVLLGFLPLLLFVGFFIYLGR
                     STRRQLAGLGGVGRSRAKVYDEERPSTTFADVAGYEGAKREVTEVVDFLKYPDRYRRA
                     GAVGPKGVLMVGPPGTGKTLLARAVAGEAGVPFISVTGSSFVEMFVGVGAARVRDLFN
                     DARKRAPSIIFIDEVDAIGQRRGGQFVSNDERDQTLNQMLAELDGFDPATGVVVIAAT
                     NRPEVLDPALLRPGRFDRQVVIPLPTQRERRAILEVHIKGKQLGPDVDLNVVARGTPG
                     FSGADLANLVNEAAIFAVRAGREVISAVDFSEARDRVLLGRRDSSNALLPDEKRAVAV
                     HEGGHALVAALSEHGDPVAKVTILPAGQALGVTEQLPIDERHLYSEGYLKDSLAIRMG
                     GRAAELIVLGEASTGASNDLAGATDLATRMVREFGMSAALGPVGFASGSPMYLGSEEV
                     RSRPYAEATQRVIDTEVAKLLREAEERALRLLRERRGTLDQLTELLLERETIDGTDLD
                     ALLGRIPGPREPIGAGGHRATASAADDQVGG"
     gene            complement(3631..5622)
                     /locus_tag="VF995_01310"
     CDS             complement(3631..5622)
                     /locus_tag="VF995_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342240.1"
                     /translation="MCEHHESATVPDPEPNRHLNSTRRDFLRGAGRIGAALLVPGASQ
                     VLSPTIADAATVDAAAEAGLFPTRLCRVSNAMHIHASFDEVDGSFDAQLAEATRCGIN
                     VMWPTDHDWRMQAIGYITTLRFLNPEMSNGTTVGYQASTSGSLTTSTAALDSVTSPGD
                     PTGGSVHLAATSAGAEPASARMNVLPSDRLRANLTGQTLTMDVLAASVGTDAWLEVLI
                     NLSYHPPQAGRPAGNYQLSYRFGAAPAARRAQGLLGIVTVPVTPGEWNTGIALDPVSD
                     IAALWPDLVANDHHLVDLWFGATSQAGAPAEGWFANGFISRAQTGGDDPLKVQAGIFD
                     ALADRYPTVRIRHGLEISLNARHSNWFGGSFHLPVYGQDLPTNGDVTAEATALIHAAG
                     GLSSINHPFGVQSRDPATGGTSPQAAVDAARHAAALRYLGNNAYGGADIIEAGYHSRG
                     AAAQGAVAALEDHLALWDTLSRNGLFLTGNGVSDNHTGHVGVWSDDSDRNTFATHLWS
                     ASDGEADLLDALRAGRAFCAEILGGFTGALWLQVDANPMGSVSVNPAARRPLIVVATG
                     LPALGVVEVVRGPVDYAGTAQPDPATAVVATLPASAFANRGRVATVVIDTSASCFVRV
                     NVVDTVTARRTAFSNPVWLLREPPPNGIPPARRAPDTHA"
     gene            complement(5754..6428)
                     /locus_tag="VF995_01315"
     CDS             complement(5754..6428)
                     /locus_tag="VF995_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9342241.1"
                     /translation="MTAQDQYQPSERTTLRRLPERGAYDRATVHAILDEGFICHVGFA
                     VDGQPYVVPTGYARVGETLYLHGSSGSRLGLRPGMDVCVTVTLLNGLVLARSAFHHSF
                     NYKSVMALGRTRAVTDPGEKDQALRALVEHIAPGRSEAVRGGDQKELAATAVLAVPLD
                     EVSAKVRTGPPKDDQEDYDLRVWAGVLPFSVVGGEPVADERLDPAIPVPAHVRAWRRP
                     APAKDL"
BASE COUNT          963 a         2328 c         2289 g          945 t
ORIGIN      
        1 tcccgcgccg aacacccgac cctggtgctg ctcgacatca tgatgcccag gctggacggc
       61 ctggaggtct gccgcaggct gaaggccggc ccggaaacca gcgacatcgt gatcgtgatg
      121 ctcaccgccc aggcccagga gcgcgaccgc gaccacgggc tggccgccgg tgccgacgac
      181 tacttcacca agccgttctc gccgctggcg ctgctgaaca tggttgagaa cctgcgggtc
      241 cgccagaacg ccggttgagt atccccgcgc cagggtccta cggtcaggcc gagcgggacg
      301 ggacgcggat cgcgtcccag ttgaccagca gccaggtggc gccgagggcg tcgaccgtgc
      361 cggtgacgta gccagtggcc aggcggttct cgcggacgcg gatcgggtgg gcgctcgggt
      421 ccggctccag cacctggacg acctcgtcga ccaggaagcc agtcgggccg tggttggtgc
      481 tcaccaccag gatgcgtccc tcggcgccga ggtccggcag cccgagggcg acccgcagac
      541 tgaccagcgg cagcaggccg ccgttgccgt ccagcgccgc cagcgtcgag gacggcagcc
      601 cggggactgg cggctgggcg ccgacgtcga cgatcccaag cacccgctca cgcgggctgg
      661 cgtagccgcg gccgcccagc cggaacagca gcagggcggc gggaggcatc gtcatgggcc
      721 ggtcgctccg tgatcgggtc ggggtttgtt ggttttgatc gctgctttct cggcatctgg
      781 gcgcaccatc tggagcaacc ggtcagcggc gccgcgacct ggcctggaaa caggccgtcc
      841 ggaagaaggc acgcgatatc cttggcgaac ggggctcccg ccgggtagcc tccgaacgct
      901 catcaaccgc tcgggggagg tgccgcactg tggcagaggt gttcgagtcc agagagattc
      961 ccggagtctg gctgattcgg ctgcagccgg ttggagacgt gcgaggtcgc ttcatcgaga
     1021 cataccggac gtcctggctg cccttcgcaa agccgatggt gcaggggaac cgctccgact
     1081 cggtccctcg ggtgctccgc ggcctgcact atcaccgccg ccaggccgac ctctggttcg
     1141 tgcaggccgg ccgggtgacc accgtgctgg tcgacacccg catcggctcc cccaccgagg
     1201 gcaagcacac caccgtgcag atgggcggcg gtgaggatct cgccgtctac atccccgagg
     1261 ggatcgccca cggcttctac gcccacgacc acgtggtcat gacctacctg gtcaccggcg
     1321 agtacgacgg ctccgacgag ctgggcatcg cctggaacga ccccgacctc ggcatcacct
     1381 ggcccgacca ggacccgatc ctgtcggagc gggaccgcca gaacgtccgg ctcgccgacg
     1441 tccccgccga ccgccgcgtc ccctacgccg gataacggcc catttcgcat cgggcgctgc
     1501 tcgtgccgtg agcagcgccg gcgagcctga aaccgttccg ttagctgacc gctggcgcta
     1561 ccgtggaggc gacgaacctg tcgctctcca ggagcgaatc aatgctggaa cacccagagc
     1621 cgcacccgca cccgaccgag cacctgatga gcaacaccgc caagccgggc ccgccgcccg
     1681 agcggccgcc ccgcccggca ccaccgccgc cgcccaagtg gcgcggctgg ctgccgatca
     1741 tcgggctgct gctcaccatg ctgatcctgt tccttcccgg ccggatgagc cagagccagg
     1801 tcaagacgct caactaccag cccgacctga aagacaagat cactgcgaac caggtcaagt
     1861 cgatccaact cggcaccgac ggccacatcg acggcgtcct cgctgacggc acccacttcc
     1921 gctcccagta ccccgtcaac gacaccagct tctacacgct gctcaacgat cacaacgtca
     1981 cggtggtcaa ggcggtcggg tcacagacct ccctcctgga cgtgctgctc ggcttcctgc
     2041 cgctgctgct gttcgtcggc ttcttcatct acctgggccg ctccacccgg cgccagctcg
     2101 ccgggctcgg cggggtcggg cgctcccgcg cgaaggtcta cgacgaggag cggccctcca
     2161 ccaccttcgc cgacgtcgcc ggctacgagg gcgccaagcg cgaggtcacc gaggtggtcg
     2221 actttctcaa ataccccgac cgctatcggc gcgccggtgc ggtcgggccc aagggcgtgc
     2281 tgatggtcgg cccgcccggc accggcaaga cgctgctcgc ccgcgcggtc gccggggagg
     2341 ccggggtgcc gttcatctcg gtcactggct cgtcgttcgt ggagatgttc gtcggcgtcg
     2401 gtgccgcccg ggtccgcgac ctgttcaacg acgcgcgcaa gcgggcgccc tcgatcatct
     2461 tcatcgacga ggtcgacgcc atcggccagc gccgcggcgg ccagttcgtc tccaacgacg
     2521 agcgcgacca gaccctcaac cagatgctcg ccgaactgga cgggttcgac cccgccaccg
     2581 gcgtggtggt gatcgccgcc accaaccggc ccgaggtgct cgacccggcg ctgctgcggc
     2641 ccggccgctt cgaccggcag gtggtgatcc cgctgccgac ccagcgcgag cggcgcgcca
     2701 tcctggaggt ccacatcaag ggcaaacagc tcggccccga cgtcgatttg aatgtggtcg
     2761 cccgcggcac ccccggcttc tccggcgcgg acctggccaa cctggtcaac gaggcggcca
     2821 tcttcgcggt ccgggcgggc cgcgaggtga tcagcgccgt cgacttctcc gaggcgcgcg
     2881 accgggtcct gctcggccgg cgggactcct ccaacgcgct gctacccgac gagaagcgcg
     2941 cggtcgccgt ccacgagggc ggccacgccc tggtcgcggc cctgtccgag catggcgacc
     3001 cggtcgccaa ggtcaccatc ctgcccgccg gccaggccct tggcgtcacc gagcagctcc
     3061 cgatcgacga acgccacctc tactccgagg gctacctgaa ggactccctg gcgatccgca
     3121 tgggcggccg cgccgccgag ctgatcgtcc tcggcgaggc ctccaccggc gcctccaacg
     3181 acctggccgg cgccaccgac ctggccaccc ggatggtgcg cgagttcggg atgagcgccg
     3241 ccctcggccc ggtcggcttc gcctccggca gcccgatgta cctcggcagc gaggaggtcc
     3301 gcagccgccc ctacgccgag gcgacccagc gggtcatcga caccgaggtc gccaagctgc
     3361 tgcgcgaggc cgaggagcgc gccctgcggc tgctgcggga gcgccgcggc accctcgacc
     3421 agctcaccga gctgctgctg gagcgcgaga cgatcgacgg caccgacctc gacgcgctgc
     3481 tcgggcgcat ccccggaccc cgcgagccga tcggcgccgg cggccaccgc gccaccgcct
     3541 cagccgccga cgatcaggtc ggcggctgag ctgcggcctg gcccaaggcc gctcacggtc
     3601 tggcggccgc cggtgccgtg acggtcgccg tcacgcgtgg gtgtcgggcg cccggcgggc
     3661 gggtgggatg ccgttgggcg gcggctcgcg cagcagccag accgggttgg agaaggcggt
     3721 gcgccgcgcg gtcacggtgt ccacgacgtt gacgcggaca aagcacgagg cgctggtgtc
     3781 gatgaccacg gtggcgaccc tgccccggtt ggcgaacgcg ctggccggca gggtcgccac
     3841 caccgccgtc gcgggatccg gctgggccgt gccggcgtag tcgaccggcc cgcggaccac
     3901 ctcgacgacg ccgagggccg gcagtccggt cgcgacgacg atcagcgggc ggcgggcggc
     3961 ggggttcacc gagacgcttc ccatcgggtt ggcgtccacc tgcagccaca gcgccccggt
     4021 gaagccgccg aggatctcgg cgcagaacgc ccggcccgca cgcagggcgt cgagcagatc
     4081 ggcctcgccg tccgaggcgg accagaggtg agtggcgaag gtgttgcggt cgctgtcgtc
     4141 gctccagacg ccgacgtggc cggtgtggtt gtcggacacg ccgttgccgg tgaggaacaa
     4201 gccgttgcgc gacagggtgt cccacaacgc cagatggtcc tcgagcgcgg cgaccgctcc
     4261 ctgcgccgcg gcaccgcggc tgtggtagcc ggcctcgatg atgtcggcgc cgccgtaggc
     4321 gttgttgccg aggtagcgca gggcggcggc gtgacgcgcg gcatcgacgg cggcctgcgg
     4381 ggaggtgccg ccggtggcgg gatcacggct ctggaccccg aagggatggt tgatcgacga
     4441 caggcctccc gcggcatgga tcagcgcggt ggcctccgcg gtgacgtcgc cgttggtggg
     4501 gaggtcctgg ccatagacag gcaggtggaa cgacccgccg aaccagttgg agtgcctggc
     4561 gttcagagag atctccagcc catggcggat ccgcacggtc gggtagcggt cggccagcgc
     4621 gtcgaagatg ccggcctgga ccttcagcgg gtcgtcgccg ccggtctggg cacggctgat
     4681 gaacccgttg gcgaaccacc cttcggccgg cgcgcccgcc tggctggtgg caccgaacca
     4741 caggtcgacc aggtggtggt cgttcgccac caggtccggc cacagcgccg cgatgtcgct
     4801 gaccgggtcg agcgcgatcc ccgtgttcca ctcccccggg gtcaccggga cggtcacgat
     4861 gccgagcagc ccctgcgccc gccgagcggc gggtgcggcg ccgaaacggt aggagagctg
     4921 gtagttccca gccggccggc ctgcctgcgg tggatggtag gacaggttga tcagcacctc
     4981 cagccaggca tccgtcccga cgctggcggc cagcacgtcc atggtgagcg tctggccggt
     5041 caggttggcc cgcaggcggt ccgacggcag cacattcatc cgcgcgctgg ccggctccgc
     5101 gccggcgctg gtcgcggcca ggtgcaccga cccgccagtc gggtcaccgg ggctggtcac
     5161 gctgtcaagc gcggcggtcg aggtggtgag gctgccgctg gtggaagcct gatagcccac
     5221 cgtggtcccg ttggacatct ccgggttgag gaaccgcaac gtggtgatgt acccgattgc
     5281 ctgcatgcgc cagtcatggt cggttggcca catgacattg atgccgcagc gggtcgcctc
     5341 cgccagctgc gcgtcgaagc tgccatccac ctcgtcgaag ctggcatgga tatgcatggc
     5401 gttcgacacc cggcacaacc gggttgggaa cagcccagcc tcggcggcgg cgtcgacggt
     5461 ggccgcgtcg gcgatggtcg gcgacagcac ctggcttgcc cctgggacca gcagtgcggc
     5521 accgatcctg ccagcgccac ggaggaagtc tcgccgggtc gagttcaggt gccggttcgg
     5581 ctcaggatcg gggacggtcg cggattcatg atgctcgcac atggccgctc cttggccact
     5641 gggggccacg ggggattggt acatcagcgg catcggagca tactggaata aatatcttgt
     5701 aacgtgcgta gaagcacgat tgttatgcgc cggtcagcga tgtcgcggct tcgtcacaag
     5761 tccttcgctg gcgctgggcg gcgccacgca cggacatgcg ctggcaccgg gatcgccgga
     5821 tcgaggcgct catcagcgac aggctccccg ccaacaaccg agaacggcag cacccccgcc
     5881 cacacccgga ggtcatagtc ctcctggtca tccttgggcg ggccggtgcg aaccttcgcc
     5941 gaaacctcgt cgagcggcac cgcgagcacc gcggtagcag ccagctcctt ctggtcgccg
     6001 ccgcggaccg cctcgctgcg gccaggcgcg atgtgctcca ccagggcccg cagcgcttgg
     6061 tccttctcac ccggatcggt gaccgcccgg gtgcggccga gcgccatcac gctcttgtag
     6121 ttgaaggagt ggtggaacgc gctcctggcg agaaccaggc cgttgaggag ggtgaccgtg
     6181 acgcaaacgt ccatgccggg tcgcaggccg aggcggctgc cgctggagcc gtgcaggtag
     6241 agggtctcgc cgacgcgggc gtatccggtt gggacgacgt acggctggcc gtcgacggcg
     6301 aagccgacgt ggcagatgaa gccttcgtcg aggatggcgt ggacggtcgc gcggtcgtag
     6361 gcgccccgct ccgggaggcg ccggagggtc gtccgctcgc tgggctggta ctggtcctgg
     6421 gcggtcatgt cgtgtcggtc ctggtcttca cgagagcagt gcgatcaccg cccttcacgg
     6481 taccagcggc gcggcctccg acttcgccgc cgcctcagct tcggc
//
LOCUS       DASWDC010000045         8886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_843786, whole genome shotgun sequence.
ACCESSION   DASWDC010000045 DASWDC010000000
VERSION     DASWDC010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843786"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..884
                     /locus_tag="VF995_01320"
                     /pseudo
     CDS             1..884
                     /locus_tag="VF995_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905203.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
     gene            1028..1387
                     /gene="rpsP"
                     /locus_tag="VF995_01325"
     CDS             1028..1387
                     /gene="rpsP"
                     /locus_tag="VF995_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX9342242.1"
                     /translation="MAVKIRLMRMGMKKAPYYRVVVADSRSPRDGRFIENIGLYHPTY
                     EPSRIEIDTDKARSWIAKGAQPTEAVANLLTIVGVEGPAERKAAAKALRATQAEARTA
                     PKAKGAKGKGSAKAKKS"
     gene            1392..1628
                     /locus_tag="VF995_01330"
     CDS             1392..1628
                     /locus_tag="VF995_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX9342243.1"
                     /translation="MRDVVAYLARALVDDPDRVTVQEYRGQRGVSYEVRAAPADVGKL
                     IGRGGRTVKALRKLVKAVAPDGRRIEVEVLAEDE"
     gene            1636..2289
                     /gene="rimM"
                     /locus_tag="VF995_01335"
     CDS             1636..2289
                     /gene="rimM"
                     /locus_tag="VF995_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044489.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX9342244.1"
                     /translation="MNAPARLVVGRVLRPHGIGGEVLVEVLSDTPERFAGGAALDAGD
                     PDVPAALRHLTVEGARSHQGRLLVRFAGVGDRDGAESLRASLLSIPLEAARQLGPDEY
                     WRHQLIGLTVVDHDGQPRGTVTEVLPGAAHDQLQVRRPDGSEVLVPTVAALIQVDLAA
                     GRVEVDAIPGLLDPEVLGNAPNAVAEPPDPSGDSAATARSARSASGRGIVPPERHGA"
     gene            2294..3010
                     /gene="trmD"
                     /locus_tag="VF995_01340"
     CDS             2294..3010
                     /gene="trmD"
                     /locus_tag="VF995_01340"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013708727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX9342245.1"
                     /translation="MRIDIVTIFPGFFASPLATGLLGKAGERGLVEVRCHDLRDWARD
                     RHRSVDDAPFGGGAGMVMLPAPWFEALDTLLAEGPARVVLLAPDGHRFDQASAASLAR
                     EQRLILCCGRYEGIDERVRTRVDEVVSIGDFILAGGESAALVVLDTVARLVPGVMGNA
                     ASATDESFAAGLLEYPQYTRPASYRGLEVPPVLRSGDHGAVAHWRREQALERTHRCRP
                     ELLADADLTDEERRQHPPDK"
     gene            3131..3487
                     /gene="rplS"
                     /locus_tag="VF995_01345"
     CDS             3131..3487
                     /gene="rplS"
                     /locus_tag="VF995_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX9342246.1"
                     /translation="MQISDVVERAQLRDDLPDFRPGDTVKVHVRVVEGGRERIQVFQG
                     FVLRRQGGGLRETFTVRKVSFGVGVERTFPVHAPSIARVELVTRGDVRRAKLYYLRGR
                     IGKAAKIKEKRSPARA"
     gene            3484..4251
                     /gene="lepB"
                     /locus_tag="VF995_01350"
     CDS             3484..4251
                     /gene="lepB"
                     /locus_tag="VF995_01350"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9342247.1"
                     /translation="MSQHGQEPERNDLVTTEVEGDSTADSTADAPNDGSAARGEADQT
                     SEPERNGKGRQPVGPLAVVRETALLVALAILLAVIFKTFLVQAFYIPSASMTSTLEVF
                     DRVLVEKVSYRFGKVHDGDVIVFVHDTGLPPESSNPLSRFLGGLGQAIGVAAPSNRDF
                     IKRVIGTPGDRISCRDGQVYRNGQALNEPYLDPGTPTENCRPVTIPTGKLYVLGDNRT
                     NSEDSRIFGPIDRSTVVGRAFVRIWPLNHIGWLRRDR"
     gene            4334..5020
                     /locus_tag="VF995_01355"
     CDS             4334..5020
                     /locus_tag="VF995_01355"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX9342248.1"
                     /translation="MLATAVLEDRLRHVGFERVAGVDEAGRGALAGPLVAAAVILPAG
                     CPIVGLADSKLLTPERRETLAAEIRRHSLALTVVRAAPETIDRAGLQRTNLALLARAL
                     RELAGGYDYALADGFPLVNMPAPVLGVRKGDQVATCVAAASIIAKVTRDQIMTRAARR
                     IPGYGFERHKGYGTDQHWEALRELGPSAYHRFSFAGVAAPWATRARHPAVGRDAVVDD
                     QDDLASEEGQ"
     gene            5073..5369
                     /locus_tag="VF995_01360"
     CDS             5073..5369
                     /locus_tag="VF995_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HEX9342249.1"
                     /translation="MSAEDLERYEAEMELELYREYRDVLGMFQFVVETERRFYLANKV
                     DLQVRNQDTDTYFDLTMQDAWVWDMYRPARFVTKVRVVTFKDVNIEELIRKDEL"
     gene            5380..5502
                     /locus_tag="VF995_01365"
     CDS             5380..5502
                     /locus_tag="VF995_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342250.1"
                     /translation="MGFNATRRYRDKKTTDVAILVAALVIVAGLLGWVLAAGGH"
     gene            5568..7061
                     /locus_tag="VF995_01370"
     CDS             5568..7061
                     /locus_tag="VF995_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX9342251.1"
                     /translation="MGTRGRAELQRARQRQRRRRVVFAMSIVCAVVLTVVVTVAGYAH
                     FRFGQIKSVNVQGLHSVSAGKPFNLLIVGSDTRADLEDAKNFGGATAPGGQRSDVILI
                     VRVDPATHRAAMLSIPRDTVVQIAGGGRNKINAAFVNGPGQLVKTIEQNFGVQINHYL
                     LVNFDGFQGIVNAIGGIKMNFPFPSRDAFSGLKINTTGCIRLGGAQALALARSRHFMY
                     LQNGRWITADGFAPDIGRIRRQHIFLEQLARTAISKGLTNPIRANAFVGAVVHDITKD
                     SALKITDVVRLANLFRSFNPSAMPAYTLPTGNANDAALGSIQVIKLPDAQRVVNQFLG
                     RPDTTNVAASDNPAVPAVPGVAGTSVQVLNGTSHAGIARQTATSLQAAGFHTGLVGDA
                     PAANPGATRIVYAPDGAAQGQVQALRARLIGPVQLHADSTLAAGQLQIIIGAGFGGIR
                     SATTPSTSGSTGSATATSQASSQAKPPAAAKATTATLDIRPYDPRAC"
     gene            7215..7586
                     /locus_tag="VF995_01375"
     CDS             7215..7586
                     /locus_tag="VF995_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX9342252.1"
                     /translation="MSSSSIDLGRRGEALACDQLAAAGFRVLARNWCWHAGGRSGEID
                     VIAARPGLLVFCEVKTRRGHAYGSPAAAVTSAKQAQLRRLATAYLAATRYPPGRIRFD
                     VITITWARGRDPVVHHLEGAF"
     gene            7586..>8886
                     /locus_tag="VF995_01380"
     CDS             7586..>8886
                     /locus_tag="VF995_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX9342253.1"
                     /translation="MLAKLATTTLVGLTAHPVGVEVDLGRGLPAISVVGLGDTVVLQA
                     RDRVRAAFANAGFDWPDRRITISLPPADLPKQGSGFDLPIAVGLLAAARLVPANAIEA
                     MWALGELDLGGDLRPVRGVLAAALAARDGGARLLLVPSDNLHEAALVPGVAIAGARSL
                     RQVNDWLLGRGQLDPPGSSVPACRQTDAGLEDLTDVRGQPVARRALEIAAAGGHNLLL
                     VGSPGAGKTMLARRLPGLLPELERDEALAVTLVQSAAGMLRPNAPLATARPFRSPHHA
                     ISAAGLVGGGSRTPRPGEVSLAHHGALFLDELAEFPRAAREALRQPLEDGEVTIVRAG
                     ASVRFPARFALIAAANPCPCGYLGDDQRPCRCGAEELRRYARRLSGPLLDRIDLYVHV
                     DRVSAEALADTAPGEASALVRERVHAARAVQRIRNATGPGAG"
BASE COUNT         1336 a         3406 c         2882 g         1262 t
ORIGIN      
        1 tgcagcggcc cgccgccgtc cgccagctcc aggtcctcgg cgagcaggcg aagctgccgg
       61 tctacgcgcc ctacgacggc tcgggggtgg gcgatccggt ccaggtcgcg cgcgacgccc
      121 gggcggaggc cgagcggctc acccgcgacg tggtgatcgt cgacaccgct gggcggctgg
      181 ccgtcgacgc cgagatgatg gcccaggccg cggccatcag cgaggcggtc ggtcccaccg
      241 aggtcctgct ggtggccgac gccatgaccg ggcaggacgc ggtccagacc gccatcgcgt
      301 tccaggaggg ccttgacctc accggcgtgg ttctcaccaa gctcgacggc gatgcccgcg
      361 gtggtgcggc gctgtcgatc cgctcggtga ccggcgtgcc gatcaagttc gccaccaccg
      421 gcgagaagct ggccgacttc gacgccttcc atcccgaccg cctcgccggc cgcatcctcg
      481 gcatgggcga cgtgctcacc ctgatcgaga aggccgagga gctggtcgac cagcaggaag
      541 ccgagcggat gcagcgcaag ctgcgcgagg cctccttcac cctggaggat ttcctcgacc
      601 agctgcagca ggtcaagaag ctcggcccgc tcagccaggt catcggcatg ctgccgggga
      661 tgagccgcga gctgaaggac gtcgaggtcg acgaccgcga cgtcgcccgg atcgaggcga
      721 tcatccgctc gatgaccccg accgagcgca acaaccccaa aatcctcaac ggttcccgcc
      781 gcgcccgcgt cgcccgcggt tccggcacga ccacccagca ggtcaacgcc gtcctcaagc
      841 agttcgacca ggcgcggcgc atgatgcgcc agctcgccgg catgaacccc aagcgcctgc
      901 gccggatggg actgggacgc tgacagcctg tacactccag cagtcccgga cgccaccatg
      961 gcaatgacgg gggagggcag acccggccgg gcgggcgggc caccccgaga aggagcacgt
     1021 tcatcctatg gccgtgaaga tccgccttat gcgcatgggc atgaagaagg cgccctacta
     1081 tcgcgtcgtg gtcgccgact cgcgcagccc ccgtgacggc cgtttcatcg agaacatcgg
     1141 cctctaccac cccacctacg agccctcgcg gatcgagatc gacaccgaca aggcccgcag
     1201 ctggatcgcc aagggcgctc agcccaccga ggcggtcgcc aacctgctca ccatcgtcgg
     1261 ggtcgaaggc cccgccgagc gcaaggccgc cgccaaggcg ctgcgcgcca cccaggccga
     1321 ggccaggacc gcgccgaagg ccaagggggc caagggcaag gggtccgcca aggccaagaa
     1381 gtcctgacgc catgcgcgac gtggtcgctt acctggcacg ggcgctggtc gacgaccccg
     1441 accgggtgac ggttcaggag taccgcgggc agcgcggggt cagctacgag gtccgcgcgg
     1501 cgcccgctga cgtcggcaag ctcatcggac ggggcggccg caccgtcaaa gcactccgta
     1561 agctggtcaa agcggtcgct cccgatggcc gccggatcga ggtcgaggtc ctcgccgagg
     1621 acgagtgacc acccgatgaa cgctcctgcg cgcctggtcg ttggccgcgt gctgcggccg
     1681 cacgggatcg ggggagaggt gctcgtcgag gtcctctccg acacgcccga gcggttcgcc
     1741 ggcggcgccg ccctcgatgc cggcgacccc gacgtgcctg ccgcgctgcg ccacctgacc
     1801 gtcgagggcg cccgctccca ccagggcagg ctgctggtgc gcttcgccgg ggtgggcgat
     1861 cgggacgggg ccgagagctt gcgcgcgagc ctcctctcga ttccgctgga ggcggcccgc
     1921 cagctcggcc ccgacgagta ctggcggcac cagctcatcg gcctcaccgt cgtcgaccac
     1981 gacgggcagc cgcgcgggac ggtgaccgag gtcctccccg gtgccgccca cgaccagctg
     2041 caggtgcggc gccccgacgg cagcgaggtg ctcgtcccca ccgtcgcggc gctcatccag
     2101 gtcgacctcg ccgccggccg cgtcgaagtc gatgccatcc ccggtttgct cgaccccgag
     2161 gtcctcggaa acgctcccaa cgccgttgcc gagcctcctg acccatcggg ggactccgcg
     2221 gcaaccgcgc gatcggcccg cagtgcttct ggccgcggga tcgtgccccc cgaacgccac
     2281 ggggcatgac ggcatgcgca tcgatatcgt cacgattttc ccgggcttct tcgcctcgcc
     2341 gctggctacc gggctgctcg gcaaggcggg ggagcgcggg ctggttgagg tgcgctgcca
     2401 cgacctgcgc gactgggcca gggaccggca ccgtagcgtc gacgacgcgc ccttcggcgg
     2461 cggcgccggc atggtgatgc tccccgcgcc ctggttcgag gcgctcgaca ccctccttgc
     2521 cgagggccct gcccgggtcg tcctgctcgc cccggacggg caccgcttcg accaggccag
     2581 cgccgccagc ctcgcccgcg agcagcggct gatcctctgc tgcggccgct acgagggcat
     2641 tgacgagcgc gtccgcaccc gcgtcgacga ggtcgtctcg atcggcgact tcatcctcgc
     2701 cggtggcgaa agcgccgcct tggtggtgct cgacaccgtc gcccgcctcg tccccggcgt
     2761 catgggcaat gccgcctccg ccaccgacga gtcgttcgcc gccggcctcc tcgaatatcc
     2821 ccagtacacc cgcccggcca gctaccgcgg cctggaggtc cctccggtgc tgcgctccgg
     2881 cgaccacggc gcggtcgccc actggcgccg cgaacaggcg ctggagcgca cccaccgctg
     2941 ccggccggaa ctgctcgccg acgcggacct gaccgacgag gaacgccgcc agcatccacc
     3001 agacaagtag gcacgcctat actgaatccc tgaacctcgt gttaccctcg gtcgtcgtca
     3061 cgtcgcgagg gcggcgcctg acgcctcaat caccgcaatc tttgggcttt ccttgaagga
     3121 gcttcacctg atgcagatca gcgacgtggt cgaacgcgca cagctgcgcg acgacctccc
     3181 cgatttccgg ccgggtgaca ccgtcaaggt gcatgtccga gttgtcgagg gtggccggga
     3241 gcggatccag gtcttccagg gcttcgtgct gcgccggcag ggcggcggcc tgcgggagac
     3301 cttcaccgtc cgcaaggtct cgttcggcgt cggcgtggaa cgtaccttcc cagtccacgc
     3361 cccgagcatc gccagggtcg agctggtcac ccgcggcgac gtccgccgcg ccaagctcta
     3421 ctacctgcgc ggccgcatcg gcaaggcggc gaagatcaag gagaagcgca gccccgcacg
     3481 ggcatgagcc agcacggcca agagcccgag cgcaacgacc tcgtgaccac cgaggtcgag
     3541 ggcgacagca ccgccgacag caccgccgac gcccccaacg acggcagtgc cgcccggggc
     3601 gaggcggacc agacgtccga acccgagcgg aacgggaagg gcaggcagcc cgtcggcccg
     3661 ctcgcagtgg tgcgcgagac cgcgctgctg gtggcgctgg cgatcctgct cgccgtgatc
     3721 ttcaagacct tcctggtgca ggcgttctac atcccgtcgg cgtcgatgac ctcgacgctg
     3781 gaggtcttcg accgggtgct ggtcgagaag gtctcctacc ggttcggcaa ggtccacgac
     3841 ggggacgtga tcgtcttcgt ccacgacacc ggcctgccac cggaatcgtc caacccgctc
     3901 agccggtttc tcggcggact cggccaggcg attggcgtgg cggcaccctc caaccgggac
     3961 ttcatcaaac gggtcatcgg cacgcccggt gaccggatca gctgccggga cggccaggtc
     4021 taccgcaacg gtcaggccct caacgagcca tatctggatc caggcacccc gaccgagaac
     4081 tgccggccgg tcaccatccc gacgggcaag ctctacgtgc tgggggacaa ccgcaccaac
     4141 tccgaggact cccgcatctt tgggccgatc gaccgctcca ccgtggtcgg ccgggcgttc
     4201 gtccgcatct ggccgctcaa ccacattggc tggctgcgcc gcgaccgctg accgccgaac
     4261 tcccggcaga ccgtaaccgc gcggcaatgt tccgcggtaa ccgatcgtcc accgctcgca
     4321 gtagactcca tccgtgcttg ccaccgccgt gctggaagac cggctccgac acgtgggctt
     4381 cgaacgggtc gccggcgttg acgaagccgg ccggggagcc ctggccgggc cgctcgtcgc
     4441 cgccgccgtg atccttcccg cgggctgccc gatcgtcggg ctggccgact ccaagctgct
     4501 cacccccgag cggcgcgaga cgctcgccgc cgagatccgc cgccattccc tggcgctcac
     4561 cgtggtgcgc gccgctcccg agaccatcga ccgtgccggg ctgcagcgca ccaacctcgc
     4621 cctgctggcc cgggcgctgc gggaactggc gggcggctat gactacgcgc tcgccgacgg
     4681 cttcccgctc gtcaacatgc cggcgcccgt cctcggggtc cgcaaaggcg accaggtcgc
     4741 cacctgcgtc gccgccgcct cgatcatcgc caaggtcacc agggaccaga tcatgaccag
     4801 ggcggcgcgc cgcatccccg gctacggctt cgagcggcac aagggctacg ggaccgacca
     4861 gcactgggaa gcgctgcgcg agcttggtcc ctcggcctac catcgcttct ccttcgcggg
     4921 tgtcgccgcg ccatgggcga cacgggcacg gcacccagcg gtcggtcgcg acgcagtcgt
     4981 ggacgaccag gacgacctgg catcagagga ggggcaatga ggcatcacgg tgggcgcgcc
     5041 gccgcgtgga cgaccatccc ggagcgggct gaatgagcgc cgaggacctc gagcggtacg
     5101 aggcggagat ggagctggaa ctctaccgcg agtaccgcga cgtgctcggg atgttccagt
     5161 tcgtggtcga gaccgaacgc cgcttctacc tcgccaacaa ggtcgacctg caggtccgca
     5221 accaggacac cgacacctac ttcgacctca ccatgcagga cgcctgggtg tgggacatgt
     5281 accggccggc acgcttcgtc accaaagttc gcgtggtcac gttcaaggac gtgaacatcg
     5341 aggaactaat ccgcaaagac gagttataac taaccgatca tgggattcaa cgcgacccgg
     5401 cgctaccggg acaagaagac cactgacgtc gcgatcctgg tcgctgcgct tgtcatcgtc
     5461 gccggtctgc tcggctgggt cctcgcggca ggtgggcact gagcgcgcca cccgacacgg
     5521 tcaagggccc gccagtcgcg cagggccggg ctgtcgcgca gggccgcgtg ggcaccagag
     5581 gccgcgccga gttgcagcgt gcccgccagc gccagcgccg ccgccgcgtc gtcttcgcca
     5641 tgagcatcgt ctgcgccgtg gtcctgactg tcgtcgtcac ggtcgccggc tacgcccact
     5701 tccgcttcgg ccagatcaag tcggtgaacg tccaggggct ccacagcgtc agtgcgggaa
     5761 agccgttcaa cctgctgatc gtcggctccg acacccgggc tgacctcgag gacgccaaga
     5821 atttcggtgg tgccaccgcc cctggcgggc agcgcagcga cgtcatcctg atcgtccgcg
     5881 tcgatcccgc cacccaccgc gccgcgatgc tgtcgattcc ccgcgacacc gtcgtccaga
     5941 tcgccggcgg cggccgcaac aagatcaacg ctgcgttcgt caacggcccc ggccagctcg
     6001 tcaagaccat cgagcagaac ttcggggtcc agatcaacca ctatctgctg gtcaacttcg
     6061 acggcttcca gggcatcgtc aacgccatcg gcggcatcaa gatgaacttc ccgttccctt
     6121 ctcgggacgc gttctccggc ttgaagatca acaccaccgg ctgcatccgg cttggcggcg
     6181 cccaggccct ggcgttggcc cgcagccgtc acttcatgta cctccagaac ggacgatgga
     6241 tcaccgccga cgggttcgcc cctgacatcg gccggatccg ccgccagcac atcttcctcg
     6301 aacagctcgc caggaccgcg atctccaagg gcctcaccaa cccgatccgc gccaacgcct
     6361 tcgtcggtgc ggtcgtccac gacatcacca aggactcggc cctcaagatc accgacgtcg
     6421 tccggctcgc gaacctgttc cgcagcttca acccgagcgc catgccggcc tacaccctgc
     6481 caaccggcaa cgccaacgac gcggcgctcg gcagcatcca ggtcatcaag ctccccgacg
     6541 cccagcgggt cgtcaatcag tttctcggcc ggcccgacac caccaacgtg gccgccagcg
     6601 acaaccccgc tgtccccgct gtccccggcg tcgccggcac ctcggtccag gtgctcaacg
     6661 gcaccagcca cgccggcatc gcccgccaga ccgccaccag cctccaagcg gctggcttcc
     6721 acaccggcct ggtgggcgac gcgcccgctg ccaaccctgg cgccacccgc atcgtctacg
     6781 cacccgacgg cgccgcccag ggccaggtcc aagcgctccg cgctcgcctc atcggtcccg
     6841 tccagctcca cgccgacagc accctggcgg ccgggcagct ccagatcatc atcggtgccg
     6901 ggttcggcgg cattcgcagc gccaccactc cgtccaccag cggaagcacg ggcagcgcga
     6961 ccgccaccag ccaggcgagc agccaagcca agccacccgc ggccgccaag gcgacgaccg
     7021 ccaccctcga catccgcccc tacgacccgc gggcttgctg accggtcgcc cgctgattcc
     7081 ggcagcgtcg gcggctgagc ggtcgggcgg ctgctgatcg ctgcctgggc cgtcggccgc
     7141 ggctcgcgaa caaagttatc cacaattccg cccgggcctc cctacacgcc gtgaacgccg
     7201 cgtagggttc ggccatgtca tcatcctcga tcgaccttgg ccggcgcggc gaggcgctcg
     7261 cttgcgacca gctcgccgcc gccggcttcc gggtgctcgc ccgcaactgg tgctggcacg
     7321 caggtggccg ctctggcgag atcgacgtca tcgccgctcg ccccggcctc ttggtcttct
     7381 gcgaggtcaa gaccagacgc ggccatgcct acggcagccc cgccgccgcg gtcacctccg
     7441 ccaagcaggc acaactgcgc cgcctggcca ctgcctacct cgccgccacc cgctacccgc
     7501 ccggccggat ccgtttcgac gtcatcacca tcacctgggc gcgggggcgc gaccccgtcg
     7561 tccatcacct tgagggggcg ttctagtgct cgccaagctc gccaccacca cgctggtcgg
     7621 cctcaccgcc catccggtcg gggtcgaggt cgacctcggc cggggcctgc ccgccatcag
     7681 tgtcgtcggc cttggcgaca ccgtcgtgct tcaggcacgc gaccgcgtcc gcgccgcctt
     7741 cgccaatgcg gggttcgact ggccggaccg gcgcatcacc atctcgcttc cgcctgccga
     7801 cctgcccaag cagggctccg gcttcgatct gccgatcgct gtcggcctgt tggccgccgc
     7861 caggctggtc cccgccaacg ccattgaggc catgtgggcg ctcggcgagc tcgacctcgg
     7921 cggtgatctg cgcccggtac ggggcgtgct cgctgcggcg ctcgccgccc gcgatggcgg
     7981 cgcccgtctc ctgctggtcc ccagtgacaa cctgcacgag gccgcgctgg tccccggggt
     8041 cgcgatcgcc ggggcgcgct cgctccggca ggtcaacgac tggctcctgg gtcgtggcca
     8101 gctcgacccg cccggttcct ccgtgcccgc ttgtaggcaa acggacgccg gtctggagga
     8161 tctcaccgac gtccgcggcc agccggtcgc ccggcgtgcg ctcgagatcg ccgccgccgg
     8221 cggtcataac ctgctgctgg tcggctcacc gggtgcgggc aagacgatgc tggcgcgccg
     8281 cctgcccggc ctgctgcccg agctggagcg cgacgaggcc ctcgcggtca ccctggtcca
     8341 gtcggccgcc ggcatgctcc gcccgaacgc accgctggcc acggcccgtc cgttccgctc
     8401 gccccaccac gccatctcgg cggcgggtct ggtcggcggc ggctccagga caccccgccc
     8461 cggtgaggtg agcctggccc accacggcgc gttgttcctg gacgaactcg ccgagttccc
     8521 ccgcgccgcc cgcgaggcgt tgcgccagcc gcttgaggac ggcgaggtca ccatcgtccg
     8581 cgccggcgcc agtgtccggt tccccgcccg cttcgccctg atcgccgccg ccaacccctg
     8641 cccttgcggc tacctgggcg acgaccagcg tccctgccgc tgcggggcag aggaactgcg
     8701 tcgctacgcg cgccgccttt ccggcccgct gctcgaccgc atcgacctgt atgtgcacgt
     8761 cgaccgggtc agtgccgagg cccttgcgga caccgctccg ggcgaggcct cggcactggt
     8821 tcgcgaacgg gttcacgctg cccgcgccgt gcagcgcatc cgcaacgcca ccggtcccgg
     8881 agccgg
//
LOCUS       DASWDC010000046         3712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_510615, whole genome shotgun sequence.
ACCESSION   DASWDC010000046 DASWDC010000000
VERSION     DASWDC010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510615"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2736
                     /locus_tag="VF995_01385"
     CDS             <1..2736
                     /locus_tag="VF995_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX9342254.1"
                     /translation="QARMAEAARQGRVEITAMPFQLHTEACSTEELQRMLRGVIELRE
                     RYGIPVRSAMHTDVPGATVGLIDALAAVGIPYLSAAHNWAGRSVPYLVGGQELTRPFW
                     WRAPSGNRLLVWHTDTPHGIAYMEGNLVGLAAGYDLALDLVPAYLSALASRPWPFGGK
                     AFGWSGLPEGAEVTMRPYPHDIVHLRVQGSDADNAGPSLIPASIVRAWNQAWAFPRLR
                     MATNHDFLAAAEERLGGALDEFEGDWTDWWADGLGSGARPLGYARRAQRDLRAAETLH
                     ALADVRPPAENGDGDGEMGVSVGAAVDAAYDKLALFDEHSWGSANPWTDGEEGVDSGA
                     IQWARKASFAHDGADDAADLLHAGVRRLGATFARVAGALASIVVFNPSSGEGARTDLA
                     EVWVPASVVDSSVPLALVDGRDGTPVPLLAGPPETVPFRPRGRRLRFIATDLPPLGYA
                     RLDLVTGPVEAHSEAERAAVSAGLGAAGLGAAGLGSAGAAAIPVTNGVVALENEFYRV
                     GYDLTEGYIASVFDKAAGRELVNQDAVGGLNQYVRDRYATAPHFNHLSGHVVANDLTL
                     LGGRFTGHGATVVRAERTAVGERLEVELHGDGTDWIRTVVELPRGVRRVDLTNRIAKV
                     GGAAKESVFFAFPFAMQSPPAAWELAGGVGGSEVAHVPGSAHYLQYLRHWVAFEEAGM
                     TLAWATLEAPLVQFGNIHLPYAPFPPTLDLPHPEPATIWSWAMNNIWDTNFPSEQRGE
                     TTFRYALTSAAGEGTSARQLGPVAAAGLVEPLVAVLIGGPAPAAGAGALDPSGGPAAT
                     VGTSRGGPGRGTVPPEPPAAAPAAGSFVTVSDPLVQVTSLGRSRRGHALMVRLRSLAP
                     EAVEATLRFPLLRPSRAFAGTLLEEGLAELAVDAGDGAVRVRLPACGTAAVAVDL"
     gene            2780..>3712
                     /locus_tag="VF995_01390"
     CDS             2780..>3712
                     /locus_tag="VF995_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018584014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX9342255.1"
                     /translation="MRIVDIRPTTVAVPLEAPLRHSNGAHWGRFVRTIVEVEADNGLV
                     GLGEMGGGGESAEAAFRNLKDYLVGHDPLQLEALRWKIANPTASLYNNRVQQLAAIEF
                     ACIDLVGQHLGVPAHDLLGGRLRDEVPFASYLFFRYPDPADGSGEVRTIDQLVEHARK
                     LKAEHGFTVHKLKGGVFPPGYELACYRALAEALPGDRLRYDPNAVPSVEEAIRFGQAV
                     EDLDNDYLEDPTWGLAGLRRVRERVRIPLATNTVVVDFEQLAANVLQPAADVVLLDTT
                     FWGGMRACVKAAAVCETFQLGIAVHSSGELGVQLA"
BASE COUNT          478 a         1415 c         1313 g          506 t
ORIGIN      
        1 caggcgcgca tggccgaggc ggcccgccag gggcgggtcg agatcaccgc catgcccttc
       61 cagctccaca ccgaagcctg ctccaccgag gagctgcagc gcatgctgcg cggcgtgatc
      121 gagctgcggg agcgctacgg catcccagtg cgcagcgcga tgcacaccga cgtgcccggc
      181 gccaccgtcg gcctgatcga cgcgctggcg gcggtcggga tcccctacct gtcggcggcg
      241 cacaactggg cgggccgctc ggtgccctac ctggtcggcg gccaggagct gacgcggccg
      301 ttctggtggc gggcgccgag cggcaaccgc ctgctggtgt ggcacaccga cacccctcac
      361 ggcatcgcct acatggaagg caacctggtc ggcctggccg ccggctacga ccttgccctg
      421 gacctggtcc cggcctacct gtccgcgctc gccagccggc cctggccctt cggcggcaag
      481 gcgttcggct ggtcggggct gccggaaggg gccgaggtca ccatgcggcc ctacccgcac
      541 gacatcgtgc acctgcgggt ccagggcagc gacgccgaca acgccgggcc gagtttgatc
      601 cctgcgagca tcgtgcgggc ctggaaccag gcctgggcgt tcccgcgcct gcgcatggcc
      661 accaaccacg acttcctcgc cgccgccgag gagcgcctcg gcggcgcgct cgacgagttc
      721 gagggcgact ggaccgactg gtgggccgac ggcctcggct cgggcgcccg gcccctcggc
      781 tacgccaggc gggcccagcg cgacctgcgc gcggctgaga ccctgcacgc gctcgcggac
      841 gtccgccctc cagccgagaa tggcgacggg gacggggaga tgggcgtctc ggtgggcgcg
      901 gcggtggacg ccgcctacga caagctcgcc ctcttcgacg agcactcctg ggggtccgcc
      961 aacccctgga ccgacggcga ggagggggtc gactccggcg cgatccagtg ggcgcgcaag
     1021 gcgagcttcg cccacgatgg cgccgacgac gccgccgacc tgctccacgc gggcgtgcgg
     1081 cgcctgggcg cgaccttcgc gcgggtggcc ggcgcgctcg cctcgattgt ggtcttcaac
     1141 ccctccagcg gggagggcgc ccgcaccgac ctggccgagg tgtgggtgcc agccagcgtg
     1201 gtggactcct ctgtgccgtt ggcgctggtc gacggccgtg acggcacgcc cgtgccgctg
     1261 ctggccgggc cgcccgagac ggtcccgttc cgaccgcgtg ggcgccgtct ccgcttcatc
     1321 gcaacagatc tgccgccgct cggctacgcc cgcctcgacc tggtcaccgg tccggtcgag
     1381 gcgcattccg aagccgagcg ggccgcggtg tccgcgggcc tgggcgcggc gggcctgggc
     1441 gcggcgggcc tgggctccgc gggcgccgca gcaatccctg tcacgaacgg cgtggtcgcg
     1501 ctcgagaatg agttctaccg ggtcggctac gacctcaccg agggctatat cgcctcggtc
     1561 ttcgacaagg cggctggccg cgagctggtc aaccaggacg cggtcggtgg cctcaaccag
     1621 tacgtccgcg accgctacgc caccgcgccg cacttcaacc atctctccgg ccacgtggtc
     1681 gccaacgacc tgacgctgct gggcgggcgc ttcaccggcc acggcgccac cgtggtgcgc
     1741 gccgagcgga cggcggtggg ggagcggctg gaggtggagc tgcacggcga cggcaccgac
     1801 tggatccgca ccgtggtcga gctgccacgc ggcgtgcgcc gggtggacct gaccaaccgc
     1861 atcgccaagg tcggcggcgc cgccaaggag agcgtcttct tcgccttccc gttcgctatg
     1921 caaagcccgc ccgccgcctg ggagctggcc ggcggggtcg gcggcagcga ggtcgcgcac
     1981 gtgcccgggt ccgcccacta cctgcagtac ctgcggcact gggtcgcctt cgaggaggcg
     2041 gggatgacgc tggcctgggc gaccctggag gcgccgctgg tgcagttcgg caatatccac
     2101 ttgccctacg cacccttccc acccaccctc gacctgcccc accccgagcc ggccaccatc
     2161 tggtcgtggg cgatgaacaa catctgggac accaatttcc cgtccgagca gcgcggcgag
     2221 accaccttcc gctatgcgct cacctccgct gcgggcgagg gcacctctgc ccgccagctc
     2281 ggcccggtcg cggcggcggg cctggtcgag ccgctggtcg ccgtcctgat cggcggccca
     2341 gctccggcgg cgggcgctgg cgcccttgac ccatcggggg gccccgcggc cactgtggga
     2401 acctctcgcg gtggtcctgg ccgcgggacc gtgccccccg aaccccccgc cgccgcgccc
     2461 gccgccggga gtttcgtcac cgtgagcgac ccgctggtcc aggtcacctc actgggccgc
     2521 tcccgccgcg gccacgccct gatggtgcgt ttgcgctcgc tggcgccaga agcggtcgag
     2581 gcgaccctgc gcttcccgct gctgcggccg tcgcgtgcct tcgccgggac gctcctggag
     2641 gagggcctcg ccgagctggc cgtggacgcc ggggacggcg ccgtgcgggt gcggctgccc
     2701 gcctgcggga cagcggcggt ggccgtggac ctctgagttc tggagtcgtg gcaagcgtag
     2761 ccagcaagga gtgcagtcca tgaggatcgt cgacatccgg cccaccaccg tggccgtgcc
     2821 gctcgaggcg ccgctgcggc actcgaacgg cgcccactgg ggccgcttcg tccgcaccat
     2881 cgtggaggtc gaggcggaca acggcctggt cggcctcggc gagatgggcg gcgggggaga
     2941 gagcgccgag gccgccttcc gcaacctcaa ggactacctg gtcggccacg accccctcca
     3001 gctggaggcg ctccgctgga agatcgccaa ccccaccgcg agcctctaca acaaccgcgt
     3061 gcagcagctc gccgccatcg agttcgcctg catcgacctg gtcggccagc acctcggcgt
     3121 ccccgcgcac gacctgctcg gcggccggct ccgcgacgag gtccccttcg ccagctacct
     3181 cttcttccgc tatcccgacc cagccgacgg cagcggcgag gtgcgcacca tcgaccaact
     3241 ggtcgagcac gcccgcaagc tcaaggccga gcacggcttc accgtccaca agctgaaggg
     3301 cggggtgttc ccacccggct acgagctggc ctgctaccgg gcgctggccg aggcgctgcc
     3361 cggcgaccgc ctccgctacg accccaacgc cgtccccagc gtcgaggagg cgatccgctt
     3421 cggccaggcc gtcgaggacc tggacaacga ctacctggag gaccccacct ggggactcgc
     3481 cggcctgcgc cgggtacgcg agcgggtgcg gatcccgctg gccaccaaca ccgtggtggt
     3541 cgacttcgag cagctcgccg ccaacgtgct gcagcccgcc gccgacgtgg tcctgctgga
     3601 caccaccttc tggggcggca tgcgcgcctg cgtcaaggcg gcggcggtct gcgagacctt
     3661 ccagctcggc atcgccgtcc actcctccgg cgagctgggc gtgcagctcg cc
//
LOCUS       DASWDC010000047         9312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_756225, whole genome shotgun sequence.
ACCESSION   DASWDC010000047 DASWDC010000000
VERSION     DASWDC010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756225"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..355)
                     /locus_tag="VF995_01395"
     CDS             complement(17..355)
                     /locus_tag="VF995_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342256.1"
                     /translation="MSLGGRCGNCGREVLLDQLVQPGEGFRCPFCGFAFTPGYATVAP
                     GVAAQAMMAHAALVKNLAELHAMTAERLRLDPGAIHDPIRDALGTERLDSSGGPRQPD
                     RRLNPARRFG"
     gene            complement(352..762)
                     /locus_tag="VF995_01400"
     CDS             complement(352..762)
                     /locus_tag="VF995_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342257.1"
                     /translation="MTNILLVAATPHARERLLLALERLEATGEEVHFLEAANGNDALA
                     IAEARRPDLVVVEVGVTPYGGFGLTRDIKAMPELSCPVIVVLERLQDEWLARWSGADA
                     LVTRPVDPFALAQVATKLLGRDQAGQDRELEAAR"
     gene            complement(759..1796)
                     /locus_tag="VF995_01405"
     CDS             complement(759..1796)
                     /locus_tag="VF995_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342258.1"
                     /translation="MPPPAPRRRFGPALLAATMGVALLFGSVGLVAARAVGAHASGAS
                     TPEAAASGLLDAINRHDLNRAATYLRGEERQLVTTYHDRLNQLLAGQGVPTTTGNPLS
                     TLDLTTRDVRFQRVDGSRDVAVLEAVSGTVGIRGPNGARIEVPISQAKDQLAKASGGK
                     LTSLRLVAIRSGGHWYVDLLATGAELGRLADGGASVDYASLTADNSAAGATSAAGAVR
                     TAISTLATDPTQALERLAPDERAVLHTYAGALRQLKRQPSEPITVEGLTTRTERVASD
                     VTKVYLTGGRVRGPGGEVTPLTSLPNELGASSGERQPYAVAVEQNGSWYPSLLFTLTD
                     YALSTAQREQS"
     gene            2441..2716
                     /locus_tag="VF995_01410"
     CDS             2441..2716
                     /locus_tag="VF995_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018223180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX9342259.1"
                     /translation="MSMEMDTPPASAPPVDAPRVVDCLGLACPLPIVRLAKAIAEIEG
                     GGVLDLLADDPGARVDIPVWCRLKDHTFLGRDDHPGGGWVFHIRRVH"
     gene            2880..4211
                     /locus_tag="VF995_01415"
     CDS             2880..4211
                     /locus_tag="VF995_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEX9342260.1"
                     /translation="MHRQRATIQRSAPGQSRTPVTVTLDAATGRDQLARLVARTARRS
                     IRISGRGQGSTLPGRLAERVSPGVLARAAKGLDPLVLVSGTNGKTTTTALLSAALRAA
                     GHRVVTNATGSNLRSGLTSALVCHRGPADCAVLEVDEATMPRVVGDLRPNLLVLLNLS
                     RDQLDRYHEVRSVGAAWRTAATRLGSHARVVAVDHDPLVCYAAQGAPEAVMVGVSGAH
                     LGRDHAGCPACGDLLGSNRGRAACPGCQWQPGPRQVEAFRQGSLVRLRGLGEQVHLSL
                     PVASPGYAANATAAWAAAVTLGVDATAAARAIAGVKVVEARYAEVGWRGRTVRLLLAK
                     NPAGWDEVLAAVAGSERPAVVSLEAGIPDGRDTSWFWDVDIRALRNRPVVVASGSRAR
                     DVALRLGVAGVNYEVEPRLDRALALAGGKGGALDLLADYTSFQAARRLVGG"
     gene            4231..5073
                     /locus_tag="VF995_01420"
     CDS             4231..5073
                     /locus_tag="VF995_01420"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX9342261.1"
                     /translation="MPPGKPGTAPIRIGIVFPQLLGTYGDRGNAEVLAWRLNARGYPA
                     EVVDLLAPDPIAPGLDAYILGGGEDLLQASAVKLLLGPEGRGLRAALAGGAPALAVCG
                     SLQLLGRAYLDGAGRKVPGLQLLDLETTASGTRCVGEMVARMADGTLLTGFENHGGRT
                     RLGAGLQPLGTVLRGHGNNGEDHTEGVRQGNLIATYLHGPILARNPALADMIAVTILR
                     RRGIDAPLPLPPIDDDPGEALHNERLEAAGFGQRRSGRLRLHARPAPSGRDRAAATEP
                     HAGS"
     gene            5082..6167
                     /locus_tag="VF995_01425"
     CDS             5082..6167
                     /locus_tag="VF995_01425"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9342262.1"
                     /translation="MPTPSPRTRPRPHHGHVHRPGRHALAPMLVLALAAAGCGHGRAR
                     NQAQAVPATSSTTTTTSAATTTTKAATKTSGHAATKPKPQAAGPARVIVVVEENHSYS
                     QIIGNPSAPFINQLAGHGTLLTGYFAVSHPSLPNYLAMTSGDTQGITSDCPTCTVPAD
                     NLGAQLNHAGIRWKAYAQSLPGPCSNTPNAGDYARKHEPFMYYDTVRNDGSCGARVVP
                     TSQLDADVAAGTLPSFAFVTPDILHDMHGNGGQSDAQRVAAGDAWLRDLSTRLVASPA
                     WRQDTRLVVTFDEGEGAEGAAGCCGGVANGGHVLTIVTGPRVPSGQDSRPYSHYALLR
                     SIEARFGLPYLRHAGDPASTTIPAIAG"
     gene            complement(6213..7127)
                     /locus_tag="VF995_01430"
     CDS             complement(6213..7127)
                     /locus_tag="VF995_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018535231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9342263.1"
                     /translation="MPDLSQQTILVTGATDGLGRALAAELARRGATVLAHGRSRERLD
                     ETVAELEAASGGDGRVRGYLADLASLEEVAGLAAQVAKAEPRLDVLVSNAGIGSNVPG
                     GEQRLESRDGIELRFAVNYLAGYVLTRQLLPLLQDSVDPSGGPAAAMESARASSGRGT
                     VPPEPPRPARIVNVSSIGQAPIDFDDVQLTRAYSGYRAYCQSKLAQILDTITLAEQLD
                     GSGVTVNALHPATFMATKIVASPISEVAEGVEATLRLATGPALDGVSGRFFDGQREAR
                     ANQQAYDPAARQRLAEVSEQLTGISAKL"
     gene            complement(7213..7566)
                     /locus_tag="VF995_01435"
     CDS             complement(7213..7566)
                     /locus_tag="VF995_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008748399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX9342264.1"
                     /translation="MSEAIQTATPAVVSLTEAAATKVRDLLAREGQAELALRIAVEPG
                     GCSGLRYALFFDDRSLDGDVRGASNGVQVVTDRMSAPYLSGASIDYLDTLEKSGFTIE
                     NPNAQGSCACGESFH"
     gene            complement(7692..8198)
                     /locus_tag="VF995_01440"
     CDS             complement(7692..8198)
                     /locus_tag="VF995_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX9342265.1"
                     /translation="MTEGLAKQPRAVADAARQLGLDLDIREYPEGTRTAADAARAIGC
                     GVEQIVKSLVFVADKDPVLVLTSGANRVDVVKVAKRTGTVDVRKADAEEVRAATGYAI
                     GGTPPFGHPRQLTILVDPHLTTLGTLWAAAGTPRHVFPITPDDLLRATGGAVADVTLQ
                     ESRDERAV"
     gene            complement(8239..>9312)
                     /locus_tag="VF995_01445"
     CDS             complement(8239..>9312)
                     /locus_tag="VF995_01445"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX9342266.1"
                     /translation="QAARAIEAGLRRARPDAESVRLPVGDGGAGTLDAILAAGFQRVG
                     ARVTGPTGEPVDAAFAADGERAFVEMAEASGLKRLPGGRRVPRDATTYGTGELLGAAL
                     DRGARQLVLGIGGSATTDGGAGMASALGARFLDRHGAPLPPGGAALLRLAHVDLSGLD
                     PRLRDLDVTVASDVDNPLVGPEGAAAVYGPQKGATPDDVLLLDSALRRYAQVLNADLG
                     LDLAATPGAGAAGGLGAGAIAFLGGRLEPGIDLVLDLIGFHQALAGADLVITGEGKLD
                     DQSLRGKAPHGVARAATAQGVPVVALAGVVEVAERELRAAGIEEAHALTEIEPDVQRC
                     IAEAAPLLEQLAERVGRAWASLP"
BASE COUNT         1233 a         3434 c         3402 g         1243 t
ORIGIN      
        1 tctcccgccg gacagcctaa ccgaatcggc gcgcagggtt cagtcgacgg tcaggctgcc
       61 gcgggccccc cgatgagtca aggcgctcag tgccgagggc gtcgcggatc gggtcgtgga
      121 tcgcgcctgg gtcgaggcgg agccgctcgg cggtcatcgc gtgcagttcg gcgaggttct
      181 tgaccagcgc ggcgtgggcc atcatcgcct gggcggccac gccgggcgcg acggtggcgt
      241 agccgggcgt gaaggcgaag ccgcagaacg ggcagcggaa cccctcgccg ggctggacga
      301 gctggtcgag caggacctcg cggccgcagt tgccgcagcg ccccccgaga ctcacctggc
      361 ggcctccagc tctctgtcct ggccggcctg gtcgcgtccc agcaatttgg tggcgacctg
      421 ggccagcgcg aacgggtcga ctggccgggt gaccagcgcg tcagcgcctg accagcgggc
      481 cagccactcg tcctggaggc gctccaggac cacgatcacc gggcaggaca gctcgggcat
      541 tgctttgatg tcgcgggtca ggccgaagcc gccgtaggga gtgacgccga cctcgacgac
      601 aaccaggtcg ggacggcggg cctcagcgat cgccagcgcg tcgttgccgt tggcggcctc
      661 caggaagtgg acctcctcgc cggtcgcctc caagcgttcc agggcgagga ggagccgctc
      721 gcgggcgtgc ggcgtggcgg cgaccaggag gatatttgtc acgattgctc ccgttgtgcg
      781 gttgaaagcg cgtagtcggt cagcgtgaac agcagcgacg gataccagga cccattctgc
      841 tccaccgcga ccgcgtacgg ctggcgctca ccgctgctgg cgccgagctc gttcggcagg
      901 ctcgtcagcg gcgtgacctc gccgcccggg ccgcggaccc ggccgccggt gaggtagacc
      961 ttggtgacgt cgctggcgac acgctcggtt ctggtggtga gaccctcgac ggtgatcggc
     1021 tcggacggtt gccgcttgag ctgccgcagg gcgccggcgt aggtgtgcag caccgcccgc
     1081 tcgtcagggg cgagccgctc cagcgcctgg gtcgggtcgg tggcgagcgt gctgatcgcc
     1141 gtgcggacgg cacccgccgc gctggtggca ccggcggccg agttgtcagc ggtgagggag
     1201 gcgtagtcga cggaggcgcc gccgtcggcc aggcggccca gctcggcgcc ggtggcgagc
     1261 aggtcgacgt accagtggcc gccgctgcgg atcgcgacca gccgcagcga ggtgagcttg
     1321 ccgccgctcg ccttggcgag ctggtccttg gcctggctga tggggacctc gatgcgggcg
     1381 ccgttggggc cccggatgcc gacggtgccg ctgaccgctt cgaggacggc cacgtcgcgg
     1441 ctgccgtcga cgcgctggaa gcggacgtcg cgggtggtca ggtcgagggt cgacagcggg
     1501 ttgccggtgg tggtcggcac gccctgcccg gcgagcagct ggttgaggcg gtcgtggtag
     1561 gtggtgacca gctgccgctc ctcgccgcgg aggtaggtgg ccgcgcggtt gaggtcgtgg
     1621 cggttgatag cgtcgagcag gccgctcgcg gccgcctccg gggtgctggc gccgctggcg
     1681 tgggcgccga ccgcgcgggc ggcgaccagc ccgaccgagc cgaacagcag cgccacgccc
     1741 atcgtggcgg cgagcagtgc cggcccgaag cggcgccgcg gcgccggggg cggcaccggg
     1801 gcccagccct caccaagtgg atgggtgacc gtggcggtcg gcgcctcgga agcggaggtc
     1861 gggtctgggg ctgcgagctg cgcgccgcaa ccaccacaga accggtcgtc ggccggagcc
     1921 gggcgaccac agttcgggca gttcacctcg gcacctccaa gggtgcgaaa gagttttcgg
     1981 ttccccccag ttctacggta cccccgggcg gggacttcgg ctggaccgat cagtacttcg
     2041 gcttggccgt cggcccgcgc cagggaaccc ccgtcgtgtg cttccagcag ttgggcccgg
     2101 ggttgtactg ggacagtggc tcgccgcact gggggcaggc gcgcccaccg gggaaggtcc
     2161 gctggacggc tctccccgct cccggctgcg tccgaacacc ctgcaccgca cgtcccttgg
     2221 aagccatgtg accacattcc ttcgcccgcg aggcggctcg cccccatcgg gcatgtcgta
     2281 tccatagttg ggcgccaagg cccagccccc cgtcaggtcg gctaagtctg ccatctccgg
     2341 gcgccgcgac gcaaagcagt caacgtgtca aacgcgcagc tcaccgggcg cgaattggag
     2401 cggatggaag tgtcggcgcc ggcgagtgat actgagcggc atgagcatgg agatggacac
     2461 cccgccggcg agcgctccac cggtggacgc cccacgggtg gtcgactgcc tcgggcttgc
     2521 ctgcccgctc ccgatcgtgc gcctcgccaa ggcgatcgcc gagatcgagg gcggcggcgt
     2581 gctggatctg ctcgccgacg atccgggcgc ccgcgtggac atccccgtct ggtgccggct
     2641 caaggaccac accttcctcg gccgcgacga ccaccccggc gggggatggg tctttcacat
     2701 ccgacgcgtc cactaacaga gccgaatcgg ccgcggcggc caatcaactc accagacttt
     2761 catgtctggg agggtttact gccacgcgtg gtgttcgccg ggatgggaac acttcagaca
     2821 ggcccccagc cgcaggacag gtccatgaac acgcgaaaca cgctaacatc ccaacgccga
     2881 tgcacagaca acgcgccacg atccagcgca gcgccccggg ccagagcagg acgccggtga
     2941 cggtgaccct cgacgccgcg accggtcgcg accagctcgc acggctggtc gcacggaccg
     3001 cccggcgctc catccggatc agcggccgtg gtcagggctc gacgcttccc ggccgcctgg
     3061 ccgaacgggt ctcgcctggg gtgctcgccc gcgccgccaa gggcctggac ccgctggtgc
     3121 tcgtctccgg caccaacggc aagaccacca ccaccgcgct gctgagcgcc gcgctgcgcg
     3181 ccgccggcca ccgggtggtc accaacgcca ccggctccaa cctccgctcc ggcctgacct
     3241 cggcgctggt gtgccaccgc ggcccggccg actgcgccgt gctggaggtc gacgaggcca
     3301 ccatgccccg ggtggtcggc gacctgcgcc ccaacctgct ggtgctgctc aacctgagcc
     3361 gcgaccagct cgaccgctac cacgaggtcc gctccgtcgg cgcggcctgg cgcaccgccg
     3421 cgacccgact gggcagccac gcccgcgtcg tcgctgtcga ccacgacccc ctggtctgct
     3481 acgccgccca gggcgcgccc gaggcggtca tggtcggcgt cagtggggcc catctcggcc
     3541 gcgaccacgc cggctgcccc gcctgcggcg acctgctcgg ctccaaccgc ggcagggccg
     3601 cctgccccgg ctgccagtgg cagccggggc cgcgccaggt cgaggccttc cgccagggct
     3661 ccctggtacg cctgcgcggc ctcggcgagc aggtccacct ctcgctcccg gtggccagcc
     3721 ccggctatgc cgccaacgcc accgctgcct gggccgccgc cgtcaccctc ggcgtggacg
     3781 ccaccgccgc cgcccgcgcc atcgccggcg tcaaggtggt cgaggcccgc tacgccgagg
     3841 tcggctggcg cgggcgtacc gtgcggctgc tgctcgccaa gaacccggcc ggctgggacg
     3901 aggtcctcgc cgccgtcgcc ggcagcgaac gccccgcggt cgtctccctt gaggccggca
     3961 tccccgacgg ccgcgacacg tcctggttct gggacgtcga catccgcgcc ctgcgcaacc
     4021 gcccggtggt ggtcgccagc ggcagccgcg cccgcgacgt ggcgctgcgc ctgggggtcg
     4081 ccggggtcaa ctacgaggtc gaaccgcgcc tggaccgcgc cctcgccctg gccggcggca
     4141 aagggggcgc cctggacctg ctggcggact acacctcatt ccaggctgcc cgccgcctgg
     4201 tcggaggctg agaatcgagg accgaagctg atgccccctg gaaagcccgg caccgcgccg
     4261 atccgcatcg ggatcgtctt cccgcagctg ctcggcacct acggggaccg aggcaacgcc
     4321 gaggtgctcg cctggcgact gaacgcccgc ggctaccccg ccgaggtggt ggacctgctg
     4381 gcacccgacc cgatcgctcc aggcctggac gcctacatcc tcggcggcgg ggaggacctg
     4441 ctgcaggctt cggcggtcaa gctgctgctc ggcccggaag gccgcgggct gcgcgccgcg
     4501 ctcgccggtg gcgccccggc gctggcggtc tgcggcagcc tccagctgct cgggcgggcc
     4561 tacctcgacg gcgccggccg caaggtcccc ggcctccagc tgctcgacct ggagaccacc
     4621 gccagcggca cccgctgcgt cggcgagatg gtggcccgca tggccgacgg caccctgctg
     4681 accggcttcg agaaccacgg cggccgcacc cgcctgggcg cggggctgca gccgctcggt
     4741 accgtcctgc gcggccacgg caacaacggc gaggaccaca ccgaaggcgt ccgccagggc
     4801 aacctgatcg cgacctacct ccacgggccg atcctggccc gcaacccggc cctcgcggac
     4861 atgatcgccg tcaccatcct gcggcgccgc ggaatcgacg ccccgctgcc gctgccgccg
     4921 atcgacgacg acccgggcga ggccctccac aacgagcggc tggaggcggc cgggttcggt
     4981 cagcggcgca gcggtagact ccggctccac gcccgcccgg cgccctctgg ccgggaccgg
     5041 gcggccgcca cggaaccgca cgctgggagc tgaccgccca catgccgacc ccgtcccccc
     5101 ggacccggcc ccgcccgcac cacggccacg tccaccgccc tggccgccac gccctcgccc
     5161 cgatgctggt cctggccctc gccgccgcgg gctgcggcca cggccgcgcc cgtaatcagg
     5221 cgcaggcggt cccggcgacc agttcgacca ccaccacgac cagcgctgcc acgaccacca
     5281 ccaaggcggc gaccaagacc agcggccatg ccgccaccaa gccgaagccc caggccgccg
     5341 gccccgcccg cgtcatcgtg gtggttgagg agaaccactc ctacagccag atcatcggca
     5401 acccgtcggc gccgttcatc aaccagctcg ccggccacgg cacgctgctg accggttatt
     5461 tcgccgtctc gcacccgtcg ctgccgaact acctggcgat gacctccggc gacacgcagg
     5521 gcatcaccag cgactgcccg acctgcaccg tgcccgccga caacctcggc gcccagctca
     5581 accacgccgg gatcaggtgg aaggcgtacg cgcagagcct gccggggccc tgctccaaca
     5641 cgcccaacgc cggcgactac gcccgcaagc acgagccgtt catgtactac gacaccgtcc
     5701 gcaacgacgg cagctgtggc gccagggtcg tcccgaccag ccagctcgac gccgatgtgg
     5761 ccgccggcac cctgccgagc ttcgccttcg tgaccccgga catcctccac gacatgcacg
     5821 gcaacggcgg ccagtccgac gcgcagcggg tcgccgccgg cgacgcctgg ctgcgggacc
     5881 tctctacccg cctggtcgcc tcccccgcct ggcgccagga cacccgcctg gtggtcacct
     5941 tcgacgaggg cgagggagcc gagggcgccg cgggctgctg cggcggcgtc gccaacggcg
     6001 gccacgtcct caccatcgtc accggcccgc gggtccccag cggccaggac agccgcccct
     6061 acagccacta cgccctgctg cgctccatcg aggcccgttt cggcctcccc tacctgcgcc
     6121 acgcgggcga cccggccagc acgaccatcc cggcgatcgc cggatgacct tccgccggat
     6181 gaccatctgg agggcgagcg gcgcgccctg cttcagagct tcgcgctgat ccccgtgagc
     6241 tgctcggaga cctccgccag ccgctggcgc gctgccgggt cgtaggcctg ctggttcgcg
     6301 cgcgcctccc gctggccgtc gaagaaacgt ccgctgacgc cgtccagcgc cgggcctgtg
     6361 gccagccgca gggtcgcctc aacgccctcg gcgacctcgc tgatcggtga ggcgacgatc
     6421 ttggtcgcca tgaaggtggc cgggtgcaac gcgttgaccg tgacgccgct gccgtcgagc
     6481 tgctcggcga gggtgatggt gtcgaggatc tgcgcgagct tgctctggca gtaggcgcgg
     6541 tacccgctgt aggcgcgggt gagctgcacg tcgtcgaagt cgatcggcgc ctggccgatc
     6601 gagctgacgt tgacgatccg cgccggcctg gggggttcgg ggggcacggt cccgcggccg
     6661 gaagaagcac gagccgattc catggcggcc gcggggcccc ccgatggatc aactgagtcc
     6721 tgcagcagcg gcagcagctg ccgggtgagc acatagccgg cgaggtagtt gacggcgaaa
     6781 cgcagctcga tcccgtcgcg gctctccagg cgctgttcgc cgccaggaac gttcgagccg
     6841 atcccggcgt tgctgaccag cacgtccagg cgcggctcgg ccttggcgac ctgcgcggcg
     6901 aggccggcga cctcctccag cgaggccagg tcggcgaggt agccgcgcac tcgcccgtcc
     6961 ccgccgctgg ccgcctcgag ctccgcgacc gtctcgtcga gccgttcacg gctacgtccg
     7021 tgcgccagca cggtcgcgcc ccggcgggcc agctcggcgg cgagcgcgcg gccaaggccg
     7081 tcggtcgctc cggtcacgag gatcgtctgc tgtgaaagat caggcatagg atcacgctcc
     7141 tctcaacgcg gtcgccaacg cagaagcgcc ccgccggtgg ctccggcggg gcacggaagg
     7201 ctgcgagtga cgtcagtgga acgattcgcc gcaggcgcag cttccctggg cgttggggtt
     7261 ctcgatggtg aacccggact tctccagcgt gtcgaggtag tcgatggagg cgccgctcag
     7321 gtacggggcg ctcatgcggt cggtgacgac ctggacgccg ttggacgcgc cacggacgtc
     7381 gccgtcgagg gaacggtcgt cgaagaacag ggcgtagcga aggcccgagc agccacccgg
     7441 ctccacggcg atccgcaggg cgagttcggc ctggccttcg cgggcgagca ggtcgcgcac
     7501 cttggtcgcg gcggcctccg tcaggctcac gaccgccggc gtggcggtct ggatggcctc
     7561 ggacatccgt gctcctttca catccagctc gttccgtctc cagctgtcac tacgtcgtgc
     7621 cggggagatg gttgctcgtg caaagctcgt acccagcatg atagcgccca ccccgcgaga
     7681 cgacccatgg gtcaaacggc ccgttcgtcg cgggactctt gaagggtgac gtcggcgacc
     7741 gcgccgccgg tggcgcggag caggtcgtcc ggggtgatcg ggaagacgtg ccggggcgtg
     7801 ccagcggcgg cccacagggt gcccagggtg gtcaggtgcg gatccaccag gatggtgagc
     7861 tgccggggat ggccgaacgg cggggtgccg ccgatggcgt agccggtggc ggcgcggacc
     7921 tcctcggcgt cggccttgcg gacgtccacg gttccggtcc gcttggcgac cttgaccacg
     7981 tccacccggt tcgcgccgct ggtgagcacc aggacggggt ctttgtcggc gacgaagaca
     8041 agggatttga cgatctgctc gacgccgcag ccgatcgcgc gggcggcgtc ggcggcggtg
     8101 cgggtccctt ccgggtattc acggatgtcc aggtcgaggc cgagctggcg ggcggcgtcg
     8161 gcgaccgccc gcggctgctt ggcgagcccc tcggtcatgg ttgctcccct gatggctgcg
     8221 ttgatggtgt ggcccacctc acggtagcga agcccaggcg cggccgaccc gttcggcgag
     8281 ctgttcgagc agcggggcgg cctcggcgat gcagcgctgg acgtccggtt cgatctcggt
     8341 gagggcgtgg gcttcctcga tgccggcggc gcgcagctcc cgctcggcga cctcgaccac
     8401 gccggcgagg gcgaccacgg ggacgccctg ggcggtggcg gcgcgggcga cgccgtgggg
     8461 ggccttgccg cggaggctct ggtcgtcgag cttgccctcg ccggtgatca ccaggtcggc
     8521 gccggcgagg gcctggtgga agccgatcag gtcgaggacc aggtcgatgc cgggttccag
     8581 ccgtccgccg aggaaggcga tcgcgcccgc gcccagcccc ccggccgcgc ccgcgccggg
     8641 ggtcgccgcc aggtcgaggc cgaggtcggc gttgaggacc tgggcgtagc gacgcagggc
     8701 ggagtcgagc agcagcacgt cgtcgggggt ggcgcccttc tggggaccgt agacggcggc
     8761 ggcgccctcg gggccgacga gggggttgtc gacgtcgctg gcgacggtga cgtcgaggtc
     8821 gcgcaggcgc ggatcgaggc cgctcaggtc gacgtgggcg aggcgcagca gggcggcgcc
     8881 gccgggtggg agcggggcgc cgtgccggtc gaggaagcgg gcgccgagcg cgctggccat
     8941 gccggcgccg ccgtcggtgg tggcgctccc gccgatgccg aggacgagct ggcgggcgcc
     9001 gcggtcgagg gcggcgccga gcagctcgcc ggtgccgtag gtggtggcgt cgcgggggac
     9061 gcgccgcccg ccgggcaggc gcttgaggcc ggaggcttcg gccatctcga cgaaggcgcg
     9121 ttcgccgtcg gcggcgaagg cggcgtcgac cggctcgccg gtcgggccgg tcacccgggc
     9181 gccgacgcgc tggaacccgg cggcgaggat cgcgtcgagg gtacccgcgc cgccgtcgcc
     9241 gaccgggagc cgcacgctct cggcgtccgg ccgggcgcgg cgcaggccgg cctcgatagc
     9301 cctggccgcc tg
//
LOCUS       DASWDC010000048        12906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1011899, whole genome shotgun sequence.
ACCESSION   DASWDC010000048 DASWDC010000000
VERSION     DASWDC010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011899"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VF995_01450"
     CDS             complement(<1..770)
                     /locus_tag="VF995_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9342267.1"
                     /translation="MKIGVAGGTGLVGRETVAALRRSGHAAVVLARSTGVDLTTGEGL
                     AAALDGADAVIDATNTPAADSDQARAFFAATTAHLLAAEHDAGVGHHVVLSIVGVDRV
                     EGIAHYAGKREQERLALSAPVPATVVRATQFFDFAAMMVGWTRNGQSAALPPLLVQPV
                     AVADVAEVLVEAALGEPRNGISELAGPETQDLVDMARRTLAARGESLRLVPTWRGRFG
                     VATAGEVLLPGPGARIAPTTFEEWLATQATQATQATQAT"
     gene            complement(782..1612)
                     /locus_tag="VF995_01455"
     CDS             complement(782..1612)
                     /locus_tag="VF995_01455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9342268.1"
                     /translation="MTAKRGEPLERWRAELAAWAIPDEILAHATESPWGFPVGLFQER
                     AGLARSQAPTPSNQEALRHLPSGGTVLDVGAGAGAASLPLAGSAGRIVAVDESAAMLA
                     AFREEADAAKVEAGAVQGRWPDVAGEVGPADVVVCHHVLYNVADLEPFVAALTSHARH
                     RVVAEISDRHPQVGLGPLWRRFHGLERPEGPSADDAEAALRQLGLEVSRTDWEGTGGS
                     DQANWDEVIAFTRRRLCLPPGREAEVADALEEARAAGQSLPFLGDVPRRLVTLSWSGG
                     "
     gene            complement(1805..2230)
                     /locus_tag="VF995_01460"
     CDS             complement(1805..2230)
                     /locus_tag="VF995_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342269.1"
                     /translation="MSGCPLAGLVVRGTVLEVLLVELPLVELLLVELLLELLAPLEEL
                     ELVDELELLVVGRTVVVVGSTILTGGATRWRTRGAVLGVGSDAVAGDTCDRGARVVVV
                     TTIPLATARCDGGEVMSASALPEIAPTATAAVRIDHRRA"
     gene            complement(2227..3165)
                     /locus_tag="VF995_01465"
     CDS             complement(2227..3165)
                     /locus_tag="VF995_01465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX9342270.1"
                     /translation="MKEQKPAAAERGGAADSGLFGPNSASWRVLRESSVMVGGVRALL
                     MHAAHPLVVAGARQTGMYEHDPWRRLERTLRLTFTVVFGTTAEAQAASRRIDDVHRGI
                     KGIDPVTRLPYDARDPALLLWVHACLVASFLELERRTLGRLDDAGRQRFHEEQMVGVA
                     PLGLPPEGIPPTVAALDAYLQQVIASGILRRTDSAAQVAALIRSPPRQVPRRRLWRLV
                     SFLAFHTLPPELRDLYEVRHRWRHELALRMICGLMRLDRPLLSPRWRFIAPALAAEAR
                     LRGGPVQVSEVGLFAESPPRPEPSAPQGRGSGSSPG"
     gene            3278..3466
                     /locus_tag="VF995_01470"
     CDS             3278..3466
                     /locus_tag="VF995_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342271.1"
                     /translation="MMNTNSIRSFVADEELAAVRRGHERGLVTDEELTQRLGEVERIL
                     WIGSPRSPYRQSDLSVAR"
     gene            complement(3497..4282)
                     /locus_tag="VF995_01475"
     CDS             complement(3497..4282)
                     /locus_tag="VF995_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9342272.1"
                     /translation="MLIQSVGRAARILKALGSESARLGVTELGERLNLPKATVYGLLR
                     TLEAHQFVEQDLETGKYRLGPALLQLGNAFLDNHELRARSLLWAESLAGRAREAVHVG
                     VLYNDMVLIVHHVFRPDNSVQILEVGASIPWHASALGKAMAAHLDPEHRSRLLGGPLP
                     RLTGRTVTDPAELEQQLARVTHDGFATEDQEAIVGEAGIAAPLFDNRGVVVGAIGLAG
                     PVEHLLADGPAASAVAAVKEAARGVSRELGARRLAIPDTGAGA"
     gene            complement(4374..4970)
                     /locus_tag="VF995_01480"
     CDS             complement(4374..4970)
                     /locus_tag="VF995_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX9342273.1"
                     /translation="MGEIGITAGIRSRMAAPATIGGARLVDAACVRTWLEAVAERIEV
                     ERHYLTQLDAAIGDADAFGPRRLLEALRAALAGVQRVGAAAEGDKTMVDALAPGLTAF
                     EDALRGGHSPGGHSPGYGGSGAHGGLEAAARQAHAAAVEGMRATIPMQARKGRASYLG
                     PRSVGHQDPGATSTALMFAALEEAVLAQRARPQRLRKA"
     gene            complement(5003..5404)
                     /gene="dhaM"
                     /locus_tag="VF995_01485"
     CDS             complement(5003..5404)
                     /gene="dhaM"
                     /locus_tag="VF995_01485"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HEX9342274.1"
                     /translation="MVGLVVVSHSAKIAEGVVELAAQMAGEVRIRAAGGTDDGGIGTD
                     ATLIADAISAADDGEGVLVLVDLGSAVLSAQLAIDELIDSERRDRVRIADGPLVEGAV
                     VAAIQASTGSTLDEVAEAAHDAATMRKAASE"
     gene            complement(5439..6440)
                     /gene="dhaK"
                     /locus_tag="VF995_01490"
     CDS             complement(5439..6440)
                     /gene="dhaK"
                     /locus_tag="VF995_01490"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEX9342275.1"
                     /translation="MKKLINGIDDFVPESLEGLARAHADLVAVNYDPAVVYRVDAPRQ
                     GKVGLVSGGGSGHEPLHGGFVGRGMLDAACPGAVFTSPTPDQMEWATTRVDGGAGVLH
                     IVKNYSGDVMNFEMAADLARDNGVEVESVVIDDDVAVKDSLYTQGRRGVGGTVLLEKL
                     AGSKAEEGADLASVTDVARQVIAGVRSMGLALTSCTVPAAGSPTFDLPDDEVEIGIGI
                     HGEPGRERIKLESADALTDRIAGPVIEDLGLTSGDLVLLFVNSMGGTPLAELYVVYRH
                     VAKLLDAGGVQISRSLVGPYITSLEMQGASVTILRLDDELTRLWDAPVHTPALRWGV"
     gene            complement(6538..7338)
                     /locus_tag="VF995_01495"
     CDS             complement(6538..7338)
                     /locus_tag="VF995_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX9342276.1"
                     /translation="MRRNTLLGELSAEFLGTFTLIIFGVGVVAQTVLTPFTAGAQSIH
                     WAWGIGVTMGVYVAGGISGAHINPAVTLALAARRGFAWGKVLPYWLAQTAGAFVAALI
                     IRWNYWEAFDKFDPAKGVKSQSVFSTLPGNNVLPVSQLGALRDQIIGTAILVLVIFAI
                     TDTRNLSPGVNLAPFIIGLLVVGIGMALGADAGYAINPARDFGPRLASLFTGWTHAMD
                     DQNGNFYAWVPILGPFIGGLVGAYVYDGFIGRFLPVAEPEPGRVPETP"
     gene            7649..8215
                     /locus_tag="VF995_01500"
     CDS             7649..8215
                     /locus_tag="VF995_01500"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX9342277.1"
                     /translation="MRVFSFRPAITLRGRGFNGLRGWAGKPFHPPLTDIPVTAYLFGA
                     AFDLVSLLGRSRSWAVDFHRAATYVFIGGLAVSLLTALTGFFDWLRSTPKGTQARRTV
                     NTHAWIMITVTVLVIADVAVRLASESGAVHTGVGVLVLSLLAAALVSLGATYGGTLVY
                     DYGFNVETAGDHPVWHVSETDVFPGAPH"
     gene            complement(8178..9104)
                     /locus_tag="VF995_01505"
     CDS             complement(8178..9104)
                     /locus_tag="VF995_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9342278.1"
                     /translation="MQSRFVDLDGPVHYADFGGGGDAGAGPPLVLVHGLGGSHLNWMA
                     AAPLLARHSRVLAPDLIGFGRTPLAGRQPRIRANQRLLGRFVDEVAGRPAVLVANSMG
                     GLISLLEAAERPERVAGLVLVDPAVPRPRGVRLDREVRLGFTVFAIPGVGERFLAHRR
                     TRLGAERLVADVLRVCCVDSARVAPEVVAAMCALTQERNTLKGNDAAFLAAARSLLLT
                     LARPGPYLRVVQSVAAPTLLLQGDGDRLVPLGSIQAIARARPSWTLQILDGLGHVPHL
                     EDPERFAAAVTAWLDTQALDVSGARPGTRPSR"
     gene            complement(9223..10905)
                     /locus_tag="VF995_01510"
     CDS             complement(9223..10905)
                     /locus_tag="VF995_01510"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9342279.1"
                     /translation="MYSTAPLGTADPKRLGPYFILGRLGGGGMGVVYLGQTPERRLVA
                     IKAIREDLAHDPEFRARFKHEVRAVSGVAGLCTARMLDADTNAERPWYASEFIEGPTL
                     REAVDERGPLKGQQLLGFAVGVAEALEAIHAAGVIHRDLKPSNVLLSPTGPKVIDFGI
                     ARQAGQTALTQTGMLVGSLNWLSPEQLRGETASPASDVFAWGGLVAYAAGGEPPYGTG
                     SAEAVSQRILHDDPDLRGLHGPLLEAVRPALRKDPRQRPSPKALLERLVGGAPADPVA
                     AATDVVQRTWVQPSVQPSVQPAVSGAAAPAPPRESARERERGQRPTPAGGQQPRPRVV
                     SQPVAPSVPPRPRPAPPPFVPPQQPRPQPVPHTRTPVLPQAPGPAAPSPSRPAQVPEG
                     RAQDRDRAGTGERRDPPRSDGRIRVLADLLPGGLLRDLALVAAFAALEAEALASDDPV
                     SSIRVTSAMLLTLAGAALLGRRRAFTGFLFGIAALYGLIGHNGLPSLGDVGGYLLLAS
                     GIVLTGTLARVGRRHKAAMAAFAMAVGFAFVWGVLQLNLHIPNPNVHLPTPF"
     gene            11030..12238
                     /locus_tag="VF995_01515"
     CDS             11030..12238
                     /locus_tag="VF995_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017436944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9342280.1"
                     /translation="MADFGMTLEVREWRSRVSAFMDQHVYPNEPLLARDDDEAAACVS
                     DLRAKARAAGLWAPFIGPEAGGTGKGFLAYAYLNEVIGRSLWAQLVFGCQAPDAGNAE
                     ILQQFGTSEQRERWLAPLVAGEIRSFFSMTEPEVSGADPTGLRTRAVADGDDWVFDGH
                     KWFSSGAEGAAFGIVMAVTDPDAPPHRRASQIIVPADTPGVELVRAIPMLGHRGRDWT
                     THCEVRYHGVRVPQANTLGARGEGFRIAQKRLGPGRIHHVMRWLGQMQRAFELLCRRS
                     LEREAFGGPLADKQTVQNWIADSAAGIQACRLMTLDAAHKIDQGDEARVEISALKFYA
                     AGVLHDVVDRAIQVHGALGLTDQTPLAVMYREARGARIYDGPDEVHRMVVSRRILQVF
                     REGGTWEFAG"
     gene            12320..>12906
                     /locus_tag="VF995_01520"
     CDS             12320..>12906
                     /locus_tag="VF995_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkA"
                     /protein_id="HEX9342281.1"
                     /translation="MARGTTALIGLLAAVSIGLVVVITLLIAVIDPGGDDGGGHHPLL
                     ELWRNFIVAWDLQSVEVQGHAPFLALRLLLALGGIFLVGALIGILATGLESRLEELRK
                     GRSLVVEQGHTVLLGWSEQVFAIIPELVEANANQRRGCVAILAPKDKVEMEDELRAKV
                     GSTRNTRVVCRTGDPMDPDELGIVNPDVARAIMVLT"
BASE COUNT         1812 a         4588 c         4698 g         1808 t
ORIGIN      
        1 gtggcctggg tggcctgggt ggcctgggtg gcctgggtgg cgagccactc ctcgaaggtg
       61 gtcggcgcga tccgcgcgcc gggccctggg agcagcacct cgccggccgt cgcgaccccg
      121 aaccgcccgc gccaggtcgg gaccagccgc agtgactccc cccgcgccgc caacgtccgg
      181 cgggccatgt ccaccaggtc ctgagtctcg gggccggcca gctcactgat cccgttgcgg
      241 ggctccccca gggccgcctc gaccaggacc tcggcgacgt cggccaccgc gaccggctgg
      301 accagcagcg gcggcagcgc cgccgactgc ccgttgcggg tccacccgac catcatcgcg
      361 gcgaagtcga agaactgtgt tgcccgcacc accgtcgccg ggacaggagc ggagagggcg
      421 agccgctcct gctcccgctt gccggcgtag tgcgcgatgc cttccacccg gtccacgccg
      481 acgatcgaca acaccacgtg atggccgacg cccgcgtcgt gctcggcggc cagcaggtgc
      541 gcggtggtgg cggcgaagaa cgcgcgtgcc tggtcgctgt cggcggccgg ggtgttggtg
      601 gcgtcgatca ccgcgtcggc gccgtccagg gcggcagcca acccctcccc ggtcgtcagg
      661 tcgaccccgg tggagcgggc gagcaccacc gccgcatgcc ccgatcgccg cagcgccgcg
      721 acggtctcgc ggccgaccag tccggtcccg ccggcaaccc cgatcttcat gaccactcct
      781 ttcagccgcc gctccatgac agggtgacca ggcggcgggg cacgtcgcca aggaacggga
      841 ggctctggcc ggcggcgcgg gcctcctcca acgcgtcggc gacctcggcc tcacggccgg
      901 gagggaggca cagccggcgg cgggtgaagg cgatcacctc gtcccagttc gcctggtcgc
      961 tgccgccggt gccctcccag tcggtgcggc tgacctccag gccgagctgg cggagggccg
     1021 cctcggcgtc atcggcgctg ggcccctcgg gccgctccag gccgtggaag cggcgccaca
     1081 gcgggccgag gccgacctgc gggtggcggt cgctgatctc ggccaccacc cggtggcggg
     1141 cgtggctggt cagtgcggcc acgaacggct ccaggtcagc gacgttgtag agcacgtggt
     1201 ggcagacgac cacgtcggcg ggcccgacct cgccggccac gtcgggccag cgcccctgga
     1261 cggcgccggc ctcgaccttg gcggcgtcgg cctcctcgcg aaacgccgcc agcatcgcgg
     1321 ctgactcgtc gaccgcgacg atccgcccag cgcttccggc gagcggcagg ctggccgccc
     1381 cggcaccggc accgacgtcc aggacggtcc cgccagaggg caggtggcgc agcgcctcct
     1441 ggttggaggg cgtgggcgcc tgcgacctgg caagtcctgc ccgctcctgg aacagcccga
     1501 ccggaaaccc ccagggtgac tcggtcgcgt gggcgaggat ctcgtcgggg atcgcccagg
     1561 ccgccagctc ggcccgccag cgttccagcg gctcgccccg cttcgccgtc atgcccattc
     1621 cgcctcacct cgccacagtt gcccgcttcg ctcctgtccc gtcttgatca ggcagcctag
     1681 cggcgacccg ccgtttgccc cttgcctctt cgacgggatc ctccgaaata aggagtgatc
     1741 gagaaacgca gggcggctat gatcgaagac gaatcttccg gggggtccgg tccatgtcgt
     1801 ttgttcatgc gcggcggtgg tcgatcctga cggctgccgt ggccgtcggg gcgatctcgg
     1861 ggagcgcgct cgcgctcatc acctcccccc cgtcgcagcg ggccgttgcc agcgggatgg
     1921 tggtgaccac cacaacgcgg gcaccacggt cacaggtgtc gccggcgacg gcgtcgctgc
     1981 ccacccccag cacggcaccg cgggtgcgcc agcgggtcgc cccgccggtg aggatggtgc
     2041 tgcccaccac caccacggta cggccgacga ccagcagttc cagctcgtcg accagctcaa
     2101 gctcctcgag cggcgccagc agctccagca gcagctcgac cagcagcagc tcgaccagcg
     2161 gcagctcgac cagcagcacc tcgagcacgg tcccgcggac aaccaggccg gcgagcgggc
     2221 aaccgctcac ccaggcgatg agcccgatcc gcggccctgg ggggccgaag gctcgggccg
     2281 cggtggcgac tcggcgaaca gcccaacctc gctgacctgg accggcccgc cgcgcaggcg
     2341 tgcttcggcg gcgagcgccg gggcgatgaa gcgccaccgg ggcgagagca gggggcgatc
     2401 cagccgcatg agcccgcaga tcatccgcag cgccagctcg tgccgccagc ggtggcgaac
     2461 ctcgtagagg tcgcgcagct cgggcgggag ggtgtggaag gcgaggaagc tgaccaggcg
     2521 ccacaaccgg cgccggggca cctgccgggg cgggctgcgg atcagcgcgg cgacctgggc
     2581 ggcgctgtcg gtgcggcgca ggatcccgct ggcgatcacc tgctgaagat aggcgtccag
     2641 cgccgcgacg gtcggcggga tgccctccgg cggcaggccc agcggcgcca cgccgaccat
     2701 ctgctcctcg tggaagcgct gacggccggc gtcgtcgagc cggccgaggg tgcgccgctc
     2761 cagctccagg aacgaggcga ccaggcaggc gtgcacccac agcagcagcg ccgggtcgcg
     2821 ggcgtcatag ggcagccgag tcaccgggtc gatccccttg atcccccggt ggacgtcgtc
     2881 gatccgcctg gaggcggcct gggcctcagc ggtggtgccg aagacgaccg tgaaggtcag
     2941 ccgcagggtg cgttccaggc gtctccaggg atcgtgctcg tacatgccgg tctggcgggc
     3001 gccggcgacc accagcgggt gggcggcgtg catcagcaac gccctgaccc cgccgaccat
     3061 caccgagctt tcacgcagga cccgccagga ggcgctgttc ggcccgaaca acccggaatc
     3121 ggccgctcca ccccgctccg ccgccgcggg tttctgctcc ttcatctctt taaggtaccg
     3181 gttgtgcggc taaaggctcc gccagtttgc gcttgagctg gccaaacgaa agaatttcgc
     3241 ttgcacctgt cgttgagggg acctcaagca cttggggatg atgaacacga acagcattcg
     3301 gtcgtttgtc gccgacgagg agctggctgc ggtgcgccgc ggccatgagc gtggcctggt
     3361 caccgacgag gagctcaccc agcgccttgg cgaggtggag cggatcctgt ggatcggctc
     3421 gcctcggtcg ccctatcgac aaagcgattt gtcggtcgcg cggtaagcgc gagcgggcgg
     3481 tagcgcagaa tcgggctcag gcgcccgcgc cagtgtcagg gatggcgagg cggcgggcgc
     3541 cgagttcgcg ggagacgcca cgggcggctt ccttgaccgc ggcgacggcg ctggcggcgg
     3601 gtccgtcggc cagcagatgc tcgaccgggc cggccagccc gatggcgccg acgaccaccc
     3661 cgcggttgtc gaacagcggc gcggcgatcc ccgcctcccc aacaatcgcc tcttggtcct
     3721 cggtggcgaa cccgtcgtgg gtgaccctgg cgagctgctg ctccagctcg gcggggtcgg
     3781 tgacggtccg gccggtgagg cgtggcagcg gcccgccgag cagccggctg cggtgctccg
     3841 ggtcgagatg ggcggccatc gccttgccca gcgcggaggc gtgccacggg atcgaggcgc
     3901 cgacctcgag gatctgcacg gagttgtccg gccggaacac gtggtggacg atcagcacca
     3961 tgtcgttgta gagcaccccg acgtggacgg cctcgcgggc acgcccggcc agggactcgg
     4021 cccacagcag cgatcgggcg cgcagctcgt ggttgtcgag gaaggcgttg ccgagctgca
     4081 gcagcgccgg gccgagccgg tacttgccgg tctccaggtc ctgctcgacg aactggtggg
     4141 cctccagggt ccgcagcagc ccgtacacgg tggccttggg gaggttgagg cgctccccca
     4201 gctcggtcac gccgagccgg gccgactcgg agccgagggc tttgaggatg cgggcggcac
     4261 gcccgaccga ctggatcaac atggtctcac tcgtggtcgc actcgtagtc tcacttcaac
     4321 ctttggcgtt cgctttacgg ttacctccgc tacctccacc ttttacctca ttctcaggcc
     4381 ttgcgcagcc gctgtgggcg cgcgcgctgg gcgaggacgg cttcttcgag cgcggcgaac
     4441 atcagcgcag tcgaggtggc gccggggtcc tgatggccga cgctgcgggg gcccaggtag
     4501 gaggcgcgcc ctttgcgggc ttgcatgggg atggtggcgc gcatcccctc cacggcggcg
     4561 gcgtgggcct ggcgggctgc cgcctccagg ccgccgtgcg ccccgctccc gccgtagccg
     4621 gggctgtggc cgccggggct gtggccgccg cggagtgcgt cctcgaaggc ggtcaggccc
     4681 ggggcgagcg cgtcgaccat ggtcttgtcg ccctcggcgg ccgcgccgac ccgctggacg
     4741 ccggccagcg ccgcccgcag ggcctccagc agccgccgcg gcccgaacgc gtcggcgtcg
     4801 ccgatggcgg cgtcgagctg ggtcaggtag tgccgctcca cctcgatccg ctcggcgaca
     4861 gcctcaagcc aggtccggac gcaggcggca tccaccagcc gggcacctcc gatggttgcg
     4921 ggtgcggcca tcctactgcg tattccggcg gtgatgccga tctcgcccat ccgtataggt
     4981 tctgctgctt cgcagacgcg gactattcgg aggcggcctt ccgcatggtc gccgcgtcgt
     5041 gggcggcctc ggcgacctcg tcgagggtgg agccggtgct ggcctggatg gcggcgacca
     5101 ccgcgccctc gaccaggggc ccgtcggcga tgcggacacg gtcgcggcgc tcgctgtcga
     5161 tcagctcgtc gatggcgagc tgggccgaca gcaccgccga gccgaggtcg accagcacca
     5221 gcacgccctc gccgtcgtcg gcggcgctga tcgcgtcggc gatcagcgtg gcgtcggtgc
     5281 cgatgccgcc gtcgtcggtg ccgccggccg cccggatgcg gacctcgccc gccatctggg
     5341 cggccagctc gaccacgccc tcggcgatct tggccgagtg cgacaccacc acgaggccga
     5401 ccatctcgtg ccctcgcccg cgcagcctcg gccccggcct agacgcccca gcgcaatgcc
     5461 ggggtgtgga ccggggcgtc ccacaggcgg gtcagctcgt cgtcgaggcg caggatcgtc
     5521 accgaggcgc cctgcatctc cagcgaggtg atgtaggggc cgaccaggct gcggctgatc
     5581 tgtaccccgc cggcgtcgag cagcttggcg acgtggcggt agacgacgta cagctcggcc
     5641 aggggcgtgc cgcccatcga gttgacgaac agcagcacca ggtcacccga ggtgagcccg
     5701 aggtcctcga tcaccggccc ggcgatccgg tcggtgagcg cgtcggcgga ttccagcttg
     5761 atgcgctcgc ggccgggctc gccgtggatg ccgatgccga tctcgacctc gtcgtcgggc
     5821 aggtcgaagg tgggactgcc ggccgccggg acggtgcagg aggtgagcgc cagccccatg
     5881 gagcggactc ccgcgatcac ctggcgagcc acgtcggtca cgctggcgag gtcggcgccc
     5941 tcctcggcct tggacccggc gagcttttcc agcaggaccg tcccgccgac gccgcggcgg
     6001 ccctgggtgt agagcgagtc cttgaccgcg acgtcgtcgt cgatcaccac gctctccacc
     6061 tcgacgccgt tgtcgcgggc gaggtcggcg gccatctcga agttcatcac gtcgccggag
     6121 tagttcttga cgatgtgcag caccccggcg ccgccgtcga ccctggtggt cgcccactcc
     6181 atctggtcgg gggtcggcga ggtgaacacc gcccccgggc aggcggcgtc gagcatgccc
     6241 cggccgacga agccgccgtg gagcggttcg tggccggagc cgccgccgct gaccagtccg
     6301 accttgccct gccgtggggc gtcgacccgg tagaccaccg ccgggtcgta gttcacggcg
     6361 acgaggtcgg cgtgagcacg agccagcccc tccagtgact cgggcacgaa gtcgtcgatg
     6421 ccgttgatca gcttcttcat cgctgcctcc tgacgggcga cggtggaccg tccgcggtcg
     6481 gatgctggtc aggtcgtgtg ctggtcaggt cctggtcagg tcctggtcag gtcctggtca
     6541 gggcgtttcg gggacgcggc cgggctcggg ttcggcgacc gggaggaacc gcccgatgaa
     6601 cccgtcgtag acgtaggcgc ccaccaaccc gccgatgaac gggcccagga tcggcaccca
     6661 ggcgtagaag ttcccgttct ggtcgtccat cgcatgggtc cagccggtga acaggctcgc
     6721 cagcctcggc ccgaagtcac gggccgggtt gatggcatag ccggcatcgg cgccaagggc
     6781 catgccgatg cccaccacca gcaggccgat gatgaacggg gcgaggttga cgcccggcga
     6841 gaggttgcgg gtgtcggtga tcgcgaagat caccagcacc aggatggccg tgccgatgat
     6901 ctggtcgcgc agcgcgccga gctggctgac cggcaggacg ttgttgccgg gcagggtgga
     6961 gaacacgctc tgggacttga cgcccttcgc cgggtcgaac ttgtcgaagg cctcccagta
     7021 gttccagcgg atgatcagcg ccgcgacaaa cgcgccggcc gtctgggcca gccagtacgg
     7081 cagcaccttg ccccacgcga acccgcggcg ggccgccagg gcgagggtga cggcggggtt
     7141 gatgtgggcg cccgagatgc cgcccgcgac gtagacgccc atggtgacgc cgatccccca
     7201 cgcccagtgg atcgactggg cgccggcggt gaagggagtg aggacggtct gggcgacgac
     7261 gccgacgccg aagatgatca gggtgaaggt gccgaggaac tcggcggaca gctcacccag
     7321 gagggtgttg cgtctcaacg gtgcctcctt gcagccgact cggcgctgga cagcaatccg
     7381 atgctcgttc gacgatgctg aacagcgtta gataattacg aagccgcagg cttgccttgt
     7441 caaggctcca ctgtcttgat cggccgatcg gctcggatgc gtaagcccgg gcggcccggg
     7501 gtaggaggct ggtgattgca gccggttgca ttggtcccgg aacgcggcga ggatacgccc
     7561 ccaggcgatt cgttccgttt gcacatacag gttgctgccc gcgacagcgc tcgcgcgaca
     7621 actcggggca gcggcaagga ggcaggcgat gcgcgtcttc tcgttccgcc cggcgatcac
     7681 cctccgcggc cgcggcttca acgggctgcg gggctgggcc ggcaagccgt tccacccgcc
     7741 gctcaccgac atcccggtca cggcctacct gttcggcgcc gccttcgatc tggtctcgtt
     7801 gctcggccgc agccggtcat gggcggtgga cttccaccgt gccgccacct acgtcttcat
     7861 cggcgggctg gcggtctcgc tgctgaccgc gctgaccggg ttcttcgact ggctgcgctc
     7921 gaccccgaag ggcacccagg cgcggcgcac ggtcaacacc cacgcctgga tcatgatcac
     7981 cgtcacggtc ctggtgatcg ccgacgtcgc ggtgcggctc gccagcgagt ccggcgccgt
     8041 ccacaccggc gtgggcgtgc tggtgctgtc gctgctggcc gccgccctgg tgtcgctggg
     8101 cgcgacctac ggcggcacgc tcgtctacga ctacggcttc aacgtcgaga ccgccgggga
     8161 ccacccggtc tggcacgtca gcgagacgga cgtgttcccg ggcgcgcccc actgacgtcg
     8221 agggcctggg tgtcgagcca ggcggtcacc gccgccgcga accgttccgg gtcctccaga
     8281 tggggcacgt gcccgaggcc atcgaggatc tggagggtcc aggagggccg ggcgcgcgcg
     8341 atcgcctgga tcgaacccaa gggcaccagc cggtcgccgt cgccctgcag cagcagggtc
     8401 ggcgccgcca ccgactggac gacccgcagg taggggcctg gccgggcgag ggtcagcagc
     8461 agcgagcggg ccgccgccag gaaggccgcg tcgttgcctt tgagcgtgtt gcgttcctgg
     8521 gtgagcgcgc acatggcggc gaccacctcg ggggcgaccc gcgccgagtc cacgcagcag
     8581 acccgcagga cgtcagcgac cagccgctcg gcaccgagcc gggtgcggcg gtgcgccagg
     8641 aagcgctcgc caacgccggg gatcgcgaag acggtgaagc cgaggcgcac ctcgcggtcc
     8701 agccggactc cacgcggccg cggcacggcc gggtcgacca gcaccagccc ggcgacccgc
     8761 tcgggacgct cggcggcctc cagcaggctg atcagcccgc ccatggagtt ggcgaccagc
     8821 accgccggcc tgccggcgac ctcgtcgacg aagcggccga gcagccgctg gttggcgcgg
     8881 atgcgcggct gccgcccggc cagcggggtg cgcccgaagc cgatcaggtc gggcgcgagc
     8941 acccgcgagt gccgcgccag cagcggcgcc gccgccatcc agttgaggtg ggatccgccg
     9001 aggccgtgga ccagcaccag cggcgggcca gcgccggcgt cgccgccgcc gccgaagtcg
     9061 gcgtagtgga caggcccatc gaggtcgacg aaccgggact gcatagcctg gctcctgaac
     9121 cacgggtgcg cgttcgcttg ctcgtcttcc accgtggccc aaagttaccg gaccgtcaag
     9181 ttgttccttg ggacggttcg ctgagcggtt cgcggaacgc gctcagaacg gcgtgggcag
     9241 gtgcacgttc gggttgggga tgtgcaggtt gagctgcagg acgccccaga cgaaggcgaa
     9301 gccgaccgcc atggcgaacg cggccatggc cgccttgtgg cggcggccca cccgggccag
     9361 ggtgccggtc agcacgatcc ccgacgccag cagcaggtag ccgccgacat ccccgaggct
     9421 cggcaggcca ttgtggccga tcaggccgta gagcgcggcg atgccgaaca ggaacccggt
     9481 gaaggcgcgg cgccggccga gcagggccgc accggccagg gtcagcagca tggcgctggt
     9541 gaccctgatc gaggagaccg ggtcgtcgct ggccagcgcc tcagcctcga gcgcggcgaa
     9601 cgcggccacc agtgccaggt cccgcaacag ccccccgggg agcaggtcgg ccagaaccct
     9661 gatcctgccg tcggagcgcg ggggatcgcg ccgctcccct gtcccagccc ggtcccgatc
     9721 ctgggcgcgc ccttcgggta cctgggctgg ccgggagggg gagggtgcgg caggccccgg
     9781 cgcctgcggt aacaccggcg tccgggtgtg cgggacgggc tgcggccgag gttgctgcgg
     9841 cggcacgaac ggcggcggcg cgggccgtgg ccggggtggg acgctcggcg cgaccggctg
     9901 ggagacgacc cgcggccggg gctgctggcc gcctgccggt gtcggtcgtt gcccccgttc
     9961 ccgttcccgc gcgctctccc gcgggggagc gggcgccgcc gcgccgctga cggcgggctg
    10021 caccgaaggc tgcaccgaag gctgcaccca ggtgcgctgc accacgtcgg tggcggcggc
    10081 gaccgggtcg gctggggcgc caccgaccag gcgctccagc agcgccttcg ggctgggacg
    10141 ctggcgcggg tccttgcgca gcgccgggcg gacggcttcc agcagggggc cgtgcaggcc
    10201 gcgcaggtcc gggtcgtcgt ggaggatccg ctggctgacc gcctccgccg agccggtgcc
    10261 atagggcggc tcgccgccgg cggcataggc caccagcccg ccccaggcga acacgtcgct
    10321 ggccggggag gcggtctcgc cgcggagctg ctcaggggac agccagttga gcgagccgac
    10381 cagcatgccg gtctgggtga gggcggtctg gccagcctgg cgggcgatcc cgaagtcgat
    10441 caccttgggg ccggtcggcg acagcagcac gttgcttggc ttgagatcgc ggtgaatcac
    10501 cccggccgcg tggattgcct ccaacgcctc ggccacgccg acggcgaagc cgagcagctg
    10561 ctgccccttg agcggcccgc gctcgtcgac ggcctcgcgc agggtcgggc cctcgatgaa
    10621 ctcgctggcg taccacgggc gctcggcatt ggtgtcggcg tccagcatcc gcgcggtgca
    10681 caagcccgcc accccggata ccgcccggac ctcgtgcttg aagcgcgccc ggaactccgg
    10741 gtcgtgggcc agatcttcgc ggatggcctt gatcgcgacc agccgccgtt ccggcgtctg
    10801 gcccagatag acgaccccca taccgccgcc gccgagccgc cccaaaatga agtagggccc
    10861 cagccgcttt ggatcggccg ttccgagagg tgcggtgcta tacatcccaa aatcacgtta
    10921 ccaggcggct gacacgagcg agcgccagtt acgttgcggc cgggatacgg ttgtttgcag
    10981 caagaaaccg gacgggaaac gatgacggaa cccgacaccg gaggcgacca tggctgattt
    11041 cggcatgacc ctggaggtcc gcgagtggcg ctcgcgcgtc agcgcgttca tggaccagca
    11101 cgtctacccc aacgagccgc tgctggcccg cgacgacgac gaggcggctg cctgtgtcag
    11161 cgacctgcgg gccaaggcca gggcggccgg gctgtgggcg ccgttcatcg gcccggaagc
    11221 gggcggaacc ggcaaggggt tcctcgccta tgcctacctc aacgaggtga tcgggcggag
    11281 cctgtgggca cagctggtgt tcggctgcca ggccccggac gccggcaacg ccgagatcct
    11341 gcagcagttc ggcacctccg agcagcggga acgctggctc gccccgctgg tcgccgggga
    11401 gatccgctcg ttcttctcga tgaccgaacc ggaggtctcc ggcgcggacc cgacggggct
    11461 gcgcacccgc gcggtcgccg acggcgacga ctgggtgttc gacgggcaca aatggttcag
    11521 ctcgggcgcc gagggggcgg cgttcgggat cgtcatggcg gtcaccgacc cggacgcgcc
    11581 gccgcaccgg cgcgccagcc agatcatcgt cccggccgac acccccggcg tcgagctcgt
    11641 ccgcgcgatc ccgatgctcg gccaccgcgg ccgcgactgg accacccact gcgaggtccg
    11701 ctaccacggc gtgcgggtcc cccaggccaa caccctcggc gcccgcggcg aggggttccg
    11761 gatcgcccag aaacgcctcg gccccggccg catccaccac gtgatgcgct ggctcgggca
    11821 gatgcagcgc gccttcgagc tgctgtgccg ccgctcgctg gagcgggagg ccttcggtgg
    11881 cccgctggcc gacaagcaga cagtgcagaa ctggatcgcc gactccgcag ccgggatcca
    11941 ggcatgccgc ctcatgaccc tcgacgccgc ccacaagatc gaccagggcg acgaggccag
    12001 ggtcgagatc tcggcgctga agttctacgc cgccggggtc ctgcacgacg tcgtcgaccg
    12061 cgccatccag gtgcacggcg cgctcggcct caccgaccag accccgctcg cggtcatgta
    12121 ccgtgaagct cgtggggccc gtatctacga cggccccgac gaggtccacc gcatggtggt
    12181 cagccggcgc atcctccagg tcttccgtga gggcgggacc tgggagttcg ctgggtagag
    12241 cggtcgagcg cgcgatcagg gggggtcagt ggcacgctca cgttttgggc gcaaactgcg
    12301 ctattggttc gacaacacga tggcccgggg caccacggcg ctcatcgggc tgctcgcggc
    12361 ggtctccatc gggctggtgg tggtgatcac cctgctgatc gcggtgatcg acccgggcgg
    12421 ggatgacggc ggcggccacc atcccctgct ggagctgtgg cgaaacttca tcgtggcgtg
    12481 ggatctgcag tcggtggagg tgcaggggca tgccccgttc ctggcgctcc ggctgctcct
    12541 cgcgctcggt ggcatcttcc tggtcggcgc cctgatcggc atcctggcca ccgggttgga
    12601 aagccgactg gaggagctgc gcaagggccg ctcgctggtg gtcgaacagg gccacaccgt
    12661 gctgctcggc tggtccgagc aggtcttcgc gatcatcccg gaactggtcg aggccaacgc
    12721 caaccagcgc cgcggctgcg tcgccatcct ggctcccaag gacaaagttg agatggagga
    12781 cgagctgcgc gccaaggtcg gctccacgcg caacacccgg gtggtctgcc ggaccggcga
    12841 tcccatggac cccgacgagc tcgggatcgt caaccccgac gtcgcccgcg cgatcatggt
    12901 cctcac
//
LOCUS       DASWDC010000049         5289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_422606, whole genome shotgun sequence.
ACCESSION   DASWDC010000049 DASWDC010000000
VERSION     DASWDC010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422606"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /gene="hisD"
                     /locus_tag="VF995_01525"
     CDS             complement(<1..1321)
                     /gene="hisD"
                     /locus_tag="VF995_01525"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX9342282.1"
                     /translation="MLSIIDLRGSDASRDPAGLLPRAPADDLAVARASVRALVADVAA
                     RGDPAVTEAARRFDGVDTAPECWRVGADELEAAERALDPALRAALVDAIERVRAFHQA
                     QRPANLVWTDPGRPGVRLAQRFVPLSRAGVYVPGGLGAYPSSVVMNVVPAQAAGVASI
                     ALATPPGPSGRGHPAILGAAAMLGVDEIWLLGGAQAVAALACGTRTVPAADSVTGPGN
                     RYVALAKQEVAGQVRTDGHAGPTEIAIVADHTADPRLVAVDLIAQAEHDSLAACLLIT
                     DDPAIWKAVEPLLAEEVAATRHRDRVERALAGQSAVVLCDPGDRDAMLRVAEAFAPEH
                     LELLVEGAAELAARVRNAGAVFVGPWTPVALGDYAAGTNHVLPTAGTARFAAGLSTLN
                     FLRAMQVAEFDQDALAAAAPTIAALSAAEDLPAHGRAVAVRAGTAPGP"
     gene            1532..2173
                     /locus_tag="VF995_01530"
     CDS             1532..2173
                     /locus_tag="VF995_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX9342283.1"
                     /translation="MELPLFPLSSVLYPGLPIPLHIFEERYREMFGRVLAGERRFGVV
                     AIARGREVGDLPEHHPVGCVAEVREVRRYPDGRLDVVAWGERRFRIEGIAEPAPYIVG
                     DVALLPEPTGAGAERLAARVGWLFTRYLTTLLRFASEGSEQLEVPDDPVAASYLIAAG
                     LQIELADKQRLLVAETAAERLRTEAGLLRRELALLEHLHIAGPATSAVAFTPN"
     gene            2336..3565
                     /locus_tag="VF995_01535"
     CDS             2336..3565
                     /locus_tag="VF995_01535"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342284.1"
                     /translation="MIWPSPARPSTPRGAAGALLVALVVAGLALLPRQSVQPHGNGLA
                     RTPPMGWNNWNHFGCDAKTPSEALVKREADAMVASGMAAAGYQFVNIDDCWALPSRDR
                     AGNLVPDPAKFPDGISGTAAYLQAKGLKLGIYLDAGTQTCAAAIGSGFPGSLGHERQD
                     AKTIAGWGVDYLKYDNCYNGGVDATHRYVAMRDALAATGRPIVFSISEWGQNQPWAWA
                     PRVANLWRTTGDIADDWATVLSVMDANAAHAGAVAPGAWNDPDVLEVGNGGMTPAEDR
                     AHFSMWAVMAAPLIAGNDVATMSAATRAILTNREVVAVDQDPAGIQGTKVAEPAPGLQ
                     VWSKPLYDGSRAVALLNRTARTAPISARWATVGLTTSDAAVRDLWGHANRGTFAGSFT
                     ATVPSHGVVLVRVIPAA"
     gene            complement(3586..4266)
                     /locus_tag="VF995_01540"
     CDS             complement(3586..4266)
                     /locus_tag="VF995_01540"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9342285.1"
                     /translation="MTSDERWLAAAWRFVSDWLPPPPANVLEIGCGPLGGFVPELLHD
                     GYAAVGVDPEAPEGPAYHRIEFEQYEPPQPVEAVVACTSLHHVADLDDVLDRVNRMLA
                     PDGTLVVVEWARERFDEASARWCFSRLAQPAPGAEPGWLNRHRDQWDASGQPWGAYCQ
                     AWADEEGLHTGRAIVHGLEMRFERLRCADGPYFFADLAETTQADEQAAIDAGQIQAAG
                     IWYVGKRR"
     gene            complement(4291..4674)
                     /locus_tag="VF995_01545"
     CDS             complement(4291..4674)
                     /locus_tag="VF995_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018960321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9342286.1"
                     /translation="MTQKPRMALSGIVLGTPDVHELAGFYQRLLGWTVDQDEPDWMKL
                     RPPNGGPGLSFQTERDYVRPTWPAGPGDQQMMLHLDFEVDDLDAAEAQALAAGAVLAD
                     HQPEDDCRVFLDPAGHPFCLFLPDA"
     gene            4775..>5289
                     /locus_tag="VF995_01550"
     CDS             4775..>5289
                     /locus_tag="VF995_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9342287.1"
                     /translation="MRAIVVSQHGGPEVLTVRDQEPPKPGPGGLLVDVAAAGVNFMDI
                     YQRQGQPPYGGTLPVTPGGEGAGTVAAAGPEVTEFKPGDRVAWAGVGGSYAEQNVVPA
                     ERAVAVPDGVDLQVAAAVMLQGMTAHYLAGSSYPVGEGDVVVIHAAAGGVGLLLTQIV
                     KMRGGIVVATTS"
BASE COUNT          741 a         1894 c         1954 g          700 t
ORIGIN      
        1 cggggccagg ggccgtcccg gcccggaccg ccaccgcgcg gccgtgcgcc gggaggtcct
       61 cggccgccga cagggcggcg atggtggggg cggcggcggc gagggcgtcc tggtcgaact
      121 cggcgacctg catggcccgt aggaagttca gggtggacag cccggcggcg aagcgggcgg
      181 tcccggcggt gggcaggacg tggttggtcc cggccgcgta gtcgccgagg gccaccgggg
      241 tccagggccc gacgaacacc gcgccggcgt tgcggacccg ggcggccagc tcggccgcgc
      301 cctccaccag gagctccagg tgctcgggcg cgaacgcctc ggcgacccgg agcatggcgt
      361 cgcggtcgcc ggggtcgcac agcaccacgg cggattggcc ggcgagggca cgctcgaccc
      421 ggtcgcggtg gcgggtggcg gcgacctcct cggcgagcag cggctcgacc gctttccaga
      481 tggcgggatc gtcggtgatc agcaggcagg cggccagcga gtcgtgctcg gcctgggcga
      541 tcaggtcgac ggcgaccaga cgcgggtcgg cggtgtggtc ggcgacgatc gcgatctcgg
      601 tcgggccggc gtgcccgtcg gtgcggacct ggccggcgac ctcctgcttg gccagcgcca
      661 cgtagcggtt gccgggcccg gtgaccgagt cggccgccgg gacggtccgg gtgccgcagg
      721 cgagcgcggc gaccgcctgc gcgccgccga gcagccagat ctcgtccacg ccgagcatgg
      781 cggcggcgcc gaggatcgcg gggtgccccc gcccgctggg gcccggcggg gtcgccagcg
      841 cgatggacgc caccccggcg gcctgggcgg ggaccacgtt catgaccacg ctggaggggt
      901 aggcgcccag cccgcccggc acgtagacac cggcgcggga gagcggcacg aaccgctgcg
      961 ccagccgcac ccccggccgt cccgggtcgg tccagaccag gttggcgggc cgctgcgcct
     1021 ggtggaaggc ccgcacccgc tcgatcgcgt cgacgagagc ggcccgcagc gccggatcga
     1081 gggcgcgctc ggctgcctcc agctcgtcgg cgccgacccg ccagcactcc ggcgcggtgt
     1141 cgaccccgtc gaagcggcgg gccgcctcgg tcacggctgg gtcgccgcgg gcggcgacgt
     1201 cggcgaccag cgcgcggacg ctggcacggg cgacagccag gtcgtcagcg ggggcgcgtg
     1261 gcagcagccc ggccgggtca cggctggcgt cggagcctcg caggtcgatg atggatagca
     1321 cgggcacact ccatgaacga caaagcacgt ggaacggtcc acctccggag agggccaccc
     1381 taccgcagcc gccgggcacc cgcgacgcgg tttccagggg gtccagcggc cgggcgagcg
     1441 gccgggcgag cgaccgggcg agcggccagg cgagccagcg cctaccatga gtcgaattac
     1501 ggcgagattg atcgaagttg agggttcgcg cgtggaactc ccgctgttcc ccctatcgtc
     1561 cgtgctgtat ccggggctgc ccatcccgct gcacatcttc gaggagcgct accgggagat
     1621 gttcgggcgg gtcctggccg gcgagcgccg cttcggggtg gtcgcgatcg cccggggccg
     1681 cgaggtcggc gacctccccg agcaccaccc ggtcggctgc gtcgccgagg tccgcgaggt
     1741 gcgccgctac ccggacggcc gcctggacgt ggtcgcctgg ggcgagcgcc gcttccgtat
     1801 cgaggggatc gcggagccgg cgccctacat cgtcggcgac gtcgccctgc tgccggagcc
     1861 gaccggtgcc ggcgccgagc ggctggccgc ccgcgtcggc tggctcttca cccgctacct
     1921 gacgaccctg ctcaggttcg ccagcgaggg cagcgagcag ctcgaggttc ccgacgaccc
     1981 ggtggccgcc tcctacctga tcgccgccgg gctgcagatc gagctggccg acaagcagcg
     2041 cctgctggtc gccgagacag ccgccgaacg gctgcggacc gaggctggcc tgctccgccg
     2101 cgagctggcc ctgctcgagc acctccacat cgccgggccg gccacctcgg ccgtggcgtt
     2161 cacgccgaat tagaatctct cacccgtgct ggacaagctc gctgcgcacg gccagctccg
     2221 cgaggtcgcc tgacaagcgc acctgatcag ctccagcggt tgcgcgacca ggcccaggcg
     2281 cgcccgcgga gacgtcacca ggcccctggg cgaggccggg tacgatagcg ccctcgtgat
     2341 ttggccatct ccagctcgcc cctcaactcc ccgcggagcg gccggtgcgc tgctggtcgc
     2401 cctggtggtc gcggggctcg ctctgctccc tcgccagagc gtccagcccc atggcaatgg
     2461 gctggcacgg acgccgccga tggggtggaa caactggaac cacttcgggt gcgacgccaa
     2521 gacgccgagc gaagcgctgg tcaagcggga ggccgacgcg atggtggcca gcgggatggc
     2581 ggccgccggg tatcagttcg tcaacatcga cgactgctgg gcgctgccga gccgcgatcg
     2641 tgccggcaac ctcgtgcccg acccggcgaa gttccccgac gggatcagcg ggacggcagc
     2701 ctacctgcag gccaagggcc tgaagctcgg catctacctg gacgccggaa cccagacctg
     2761 tgccgccgcg atcggcagcg ggttcccggg gtcgcttggc catgagcggc aggacgcgaa
     2821 gacgatcgcc gggtggggcg tggactacct caagtacgac aactgctaca acggcggcgt
     2881 cgacgcgacc caccggtacg tggcgatgcg ggacgcgctc gcggccactg gccggccgat
     2941 cgtgttcagc atctccgagt ggggccagaa ccagccgtgg gcctgggcgc cgagggtcgc
     3001 caacctgtgg cgcaccaccg gcgatatcgc cgacgactgg gcgacggtgc tcagcgtcat
     3061 ggacgccaac gccgcgcacg ccggcgcggt cgcgccggga gcctggaacg acccggacgt
     3121 gctggaggtc ggcaacggcg ggatgacccc ggccgaggat cgggcgcatt tcagcatgtg
     3181 ggcggtgatg gcggcgcccc tgatcgccgg aaatgacgtg gccacgatgt cggccgcgac
     3241 ccgggccatc ctcaccaacc gtgaggtcgt cgcggtcgac caggacccgg ccgggatcca
     3301 gggcaccaag gtcgccgagc cggcccctgg cctccaggtc tggtcgaagc cgctctacga
     3361 cggctcgcgc gcggtcgcgc tgctcaaccg gaccgcacgt accgcgccga tcagcgcccg
     3421 ctgggccacg gtcggcctca ccacctccga cgctgccgtc cgtgacctgt ggggtcacgc
     3481 caaccggggc accttcgcgg gcagcttcac cgcgaccgtg ccctcgcatg gcgtggtgct
     3541 ggtccgcgtc atccccgccg cctgagcagc tggcgtggtg gttgatcagc gacgcttgcc
     3601 cacgtaccaa atgccagctg cctggatctg ccccgcgtcg atcgcggcct gctcgtccgc
     3661 ctgggtggtc tcggcgaggt cggcgaagaa gtacggcccg tccgcgcatc gcaggcgctc
     3721 gaacctcatc tccagcccgt gcacgatcgc ccggccggta tggaggccct cttcgtccgc
     3781 ccaggcctga cagtaggctc cccatggctg gccagaggca tcccactggt cccggtgacg
     3841 gttcagccac cccggttcag ctccgggcgc cggttgcgcc agccgggaga agcaccagcg
     3901 cgcgctggct tcgtcgaacc gctcccgtgc ccattcgacg accaccagcg tgccgtccgg
     3961 cgccagcatc ctgttgaccc ggtccagcac gtcgtcgagg tcggccacgt gatgcagcga
     4021 tgtgcaggcg accacggcct ccaccggttg tggcggctcg tactgttcga attcgatccg
     4081 atggtaggca ggtccctccg gcgcttcggg atcgaccccg accgccgcgt acccgtcatg
     4141 cagcaactcg gggacgaagc cgccgagggg cccacagcca atctcgagca cgttggccgg
     4201 cggcggcggc agccagtcac tgacgaaccg ccatgccgct gccagccacc gctcatccga
     4261 tgtcatctgc gctcccaccc tttgttgagg ctacgcgtca ggcaggaaca ggcagaacgg
     4321 gtgcccggcc gggtcgagga agacccggca gtcatcctca ggctgatggt ccgcgagcac
     4381 cgcgcccgcg gccaacgctt gggcttcggc agcgtcgagg tcgtcgacct cgaagtccag
     4441 gtgcagcatc atctgctggt cgccggggcc ggccggccag gtgggccgga cgtagtcccg
     4501 ctcggtctgg aacgagaggc ccggcccgcc gttgggcggg cgaagcttca tccagtccgg
     4561 ctcgtcctgg tccaccgtcc agccgaggag gcgctggtag aagccggcca gctcgtggac
     4621 atcgggtgtg ccaaggacga tcccgctgag agccatccgc ggtttctggg tcattgttgc
     4681 ctccagggcg gggagaagcc gacgatgcga tcttgcgatg cgatcttgca acaccaccac
     4741 gacaggtacc ggaacagaga gagggaggcg ccgcatgcgc gcgatcgtcg tcagtcagca
     4801 cggcggacca gaagtcctca ccgtccgtga ccaggagccg ccgaagccgg gtccaggagg
     4861 gctgctggtg gacgtcgccg ccgccggcgt gaacttcatg gacatctacc agcggcaggg
     4921 ccagccgccc tacggtggca ccctgccggt cacccctggc ggcgagggcg ccgggacggt
     4981 ggcggcggcc ggtcccgagg tgaccgagtt caagccgggt gaccgggtcg cctgggccgg
     5041 ggtcggcggc agctacgcgg agcagaacgt ggtgcccgcc gagcgtgccg tggcggtccc
     5101 cgacggcgtc gatctgcagg tcgcggcggc ggtgatgctg cagggcatga ccgcgcacta
     5161 cctggccggc agctcctacc cggtcggcga aggcgacgtg gtggtgatcc acgcggcggc
     5221 gggcggggtc ggcctgctgc tcacccagat cgtcaagatg cgcggcggga tcgtggtcgc
     5281 caccacctc
//
LOCUS       DASWDC010000050         7390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_89161, whole genome shotgun sequence.
ACCESSION   DASWDC010000050 DASWDC010000000
VERSION     DASWDC010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89161"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..889)
                     /locus_tag="VF995_01555"
     CDS             complement(92..889)
                     /locus_tag="VF995_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX9342288.1"
                     /translation="MSDPLLQVLQVDDLEVRPDDEAAVQRVERWFKEYAKHRDPDLRE
                     RIILSYLGLADRLADRYRGNRSVPLEDLRQVARLGLVKAVDRYEPQRSNPFIPYAIAT
                     VIGEIKRHLRDASWRLRVPRGTKDLALRLCRAVDQLPQQLGHSPTVAELAEHLGTSEE
                     EVLEAIEVARTRSAPSLDQPAGEDGDAVLGDFLEDRSSSEELENLLILPELVERLPER
                     ERRIVLLRYVDDLTQDEIAERMSMSQMHVSRLLRRALERMRVQLVEA"
     gene            complement(1094..1178)
                     /locus_tag="VF995_01560"
     tRNA            complement(1094..1178)
                     /locus_tag="VF995_01560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1142..1144),aa:Leu,seq:taa)
     gene            complement(1196..1855)
                     /locus_tag="VF995_01565"
     CDS             complement(1196..1855)
                     /locus_tag="VF995_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014092866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX9342289.1"
                     /translation="MEHLITHQVLQYGYLAVFVLMVAESACIPIPSEVTMLFGGALAN
                     AAFLASLGQQGRHLDFVTVALLGAAGNLVGSWIAWAVGYRGGRPLIDHFGRYILLRPH
                     EVDRAEVWFQRHGEAAVFVSRLLPVVRTFVSLPAGVARMRFGRFSVYTLAGSLPWTFA
                     LTAVGYALGSQWQKVERWMHPVSYMVAGLIVVAIVWWFARRLTQQRRQPLASSRRDDV
                     A"
     gene            complement(1976..2707)
                     /locus_tag="VF995_01570"
     CDS             complement(1976..2707)
                     /locus_tag="VF995_01570"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX9342290.1"
                     /translation="MAMTEAVQEYLESLYWLGEAGIEPTPANVSRAMQVSPPSVTEML
                     RRLERDGLVRRGPGKGKQITLTDEGEQVAKHVVSRHRLVEAFLVKVMGVSWDEVHEEA
                     QTFEMGVTESLEQRMLTMIGEAKTCPHGHPIGDYPRLPGVPLTSVTAGSLVRILRFEN
                     EDHDLLKLFHSRGVEPRASYTVTADPSDPEAIQLEGAGIASLPRYAARTISVSVTEPG
                     EGEPLDPGLESLAAAYLLGESHWSR"
     gene            2775..3161
                     /locus_tag="VF995_01575"
     CDS             2775..3161
                     /locus_tag="VF995_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9342291.1"
                     /translation="MRFRPVSIRIGGLPAVLVLSAALALAGAGCGSSPSQDKAATTPP
                     VSGVHDVEARSLRFSPPAIQVPAGTTVSWHFDDGAVPHNVTGKGFASPNLKQTTWSHR
                     FAAAGAFTYRCTLHANMVGRVVVTAQ"
     gene            3169..3825
                     /gene="tenA"
                     /locus_tag="VF995_01580"
     CDS             3169..3825
                     /gene="tenA"
                     /locus_tag="VF995_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEX9342292.1"
                     /translation="MSESPREPLSERLLALGEPYLRRQLDHPTVRGIGAGDLDEPRFR
                     AWLVQDYRFLLDYVRLFALAAARAPDQEALSRLVDLAHSTLHQELSLHRSYAAQFGIA
                     EADLEHAPKSRTCAAYTDFLLRTAATADFAETVAALLPCLWGYSEIGRALAAAGAPAE
                     PRYRQWVEAYADPAFAELAAWCATLLDRVTEGAGEATRKRCERAFVTSLEHEMAFWDV
                     "
     gene            complement(3833..5524)
                     /gene="treS"
                     /locus_tag="VF995_01585"
     CDS             complement(3833..5524)
                     /gene="treS"
                     /locus_tag="VF995_01585"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX9342293.1"
                     /translation="MLNDPRWYKTAVFYELYVRGFQDGNADGHGDFRGLTERLDYLQW
                     LGVDCIWLLPIFQSPLRDDGYDVSDYFSVLPEYGTLADFYEFLEAAHRRGMRVIADLV
                     MNHTSDQHPWFQESRQPGSARRDWYVWSDTDQKYQDARVIFVDTETSNWTWDPVAQAF
                     YWHRFFSNQPDLNYDNPEVQEAMLEVVRFWLDLGLDGFRLDAVPYLFEREGTNCENLP
                     ETHVFLKRLRKEVDSKYEDKLLLAEANQWPTDVVPYFGDGDECHMAFNFPVMPRMFMA
                     LRREDRTPIVEIMNRTPKIPDSAQWGLFLRNHDELTLEMVTDEERDYMYTEYAKEPRM
                     RRNIGIARRLAPLLDNGRRQIELLSSLLLSLPGSPVLYYGDEVGMGDNIYLGDRNGVR
                     TPMQWTGDRNAGFSKADPAALYLPFLMDPVYGYQAVNVEAQERVATSLLHWLRRLVQV
                     RKQHAVFGIGDYEPLYPENRRVMAFIRQLGDEVVLVVANLSRFAQPVTLDLSRWIGLL
                     PEEMFGRTPFPIIDGTPYRIAIGPHGFYWFHLVEPPADPADTATFRAIRGDVMTA"
     gene            5752..>7390
                     /locus_tag="VF995_01590"
     CDS             5752..>7390
                     /locus_tag="VF995_01590"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX9342294.1"
                     /translation="MSTSAHARDAAGGSRFRPHRTPRLAAAVVLLVGVIDLLSVLLPA
                     QRARLAALSGFVPGVVEAGASAATAAAGIGLILLAGGLRRRRRSAFVATVVLLLGSAI
                     LHVIKSLDVEEALIEAFLGGLLLGRAERFSARAGPGERGPVVRPAVALVLITYGYGLL
                     GLVINRRNVTGPLSFGRAVVEVTNMALGLGTSLHLTGRFSRFFPASVFVLFISGAVIV
                     LLRALAPVLSSRSRSPALPALVASSDDSLAYFALRDDRATIGPGDAMVSYGAASTVAL
                     AAGDPLGPREHWPAAVDAFLAEAADQGRVAAVIACGAEAMAVYEQAGLRTVYLGDEAV
                     LRLDEFSLEGRAVRIARQSWNRAKRAGYQAEVVRSGELDSATGAALQAVSRHWRGEAA
                     ERGFSMALGRLFDPRDKDTLVVMARDGGGRIRGFLHFVPWERDGVSLDVMRRERDAPA
                     ILNDFLVVEAVRRLPELGVRRMSLNFSFLRAVLAAGSQPGAPLGLRLERWVLLRLSKP
                     FQIETLYRFNKKFNPSWQPRYAAVEAIEDLPMVSMAAL"
BASE COUNT         1059 a         2544 c         2634 g         1153 t
ORIGIN      
        1 cgtagtcttc gatccgagtc atgccgctga ccgtaccacc ccgcgcgggc gctcctcggg
       61 accgatcggc gggagaaacg cgtcgcggcg gtcaggcctc caccaactgg acgcgcatgc
      121 gttccaaggc gcgccgcaac agccgcgaga cgtgcatctg ggacatgctc atccgctcgg
      181 cgatctcgtc ctgggtgagg tcgtcgacgt agcgcagcag cacgatgcgc cgctcccgct
      241 ccggcagccg ctcgaccagc tccgggagga tcagcaggtt ctccagctcc tcgctggagg
      301 agcggtcctc caggaagtcg ccgaggaccg cgtcgccgtc ctcgccggca ggctggtcca
      361 gcgacggcgc cgaccgggtc cgtgccacct cgatcgcctc caagacctcc tcctcgctgg
      421 tgccgagatg ttcggccagc tcggcgacgg tcggggagtg gccaagctgc tgcggcagct
      481 ggtcgaccgc ccggcacagc cgcaacgcca ggtccttggt gccgcgcggc acccgcagcc
      541 gccagctcgc atcgcgcagg tggcgcttga tctcgccgat cacggtcgcg atcgcgtagg
      601 ggatgaacgg gttgctgcgc tgcggctcgt agcggtcaac cgccttgacc agcccgagcc
      661 gggcgacctg gcggaggtcc tccagcggca ccgagcggtt gccgcggtag cggtcagcca
      721 gccggtcggc caggccgaga tacgacagga tgatccgttc gcgcagatcg gggtcccggt
      781 gcttcgcgta ttccttgaac caccgctcga cccgctgcac ggctgcctcg tcgtccggcc
      841 ggacctccag gtcatcgacc tggagaacct ggagcaaggg gtcagacacg accggatcgc
      901 tccgcttgcc gccattcacg ccactgccta cgaacaccat aacgctacct ctgagctgcg
      961 ggaatcctgg ccctcgggtt cagggcgggc cctgcgcggt cgcttccgcc ttacggtgcg
     1021 atcgtttccg ggaaggagat ggctaaacgc accaatcccg ctgccgcgcc gcggcagcgg
     1081 tgcccggggt cggtgccgga ggaggggatc gaaccctcat gtccttgcgg acccaacgct
     1141 tttaaggcgt ggctgtctac cagttgcagc actccggcga ggtgcgagca ggagcctacg
     1201 ctacgtcatc cctgcgcgag gaggcgaggg gctggcggcg ctgctgcgtc aggcggcggg
     1261 cgaaccacca gacgatcgcg accacgatca ggccggcgac catgtagctc accgggtgca
     1321 tccagcgttc gaccttctgc cactggctgc cgagggcgta gcccacggcg gtcagcgcga
     1381 aagtccacgg cagcgacccc gcgagggtgt agacgctgaa gcggccgaag cgcatccggg
     1441 cgacccccgc cgggaggctg acgaaggtgc ggaccaccgg cagcaggcgg gagacgaaca
     1501 ccgcggcctc gccgtggcgc tggaaccaga cctcggcgcg gtcgacctca tgcgggcgga
     1561 gcaggatgta gcggccgaag tggtcgatca gcggacggcc gccgcggtag ccgaccgccc
     1621 aggcgatcca ggagccgacc aggttgccgg cggcgccgag cagggcgacg gtgacgaagt
     1681 cgaggtggcg gccctgctgg ccgaggctgg cgaggaaggc ggcgttggcg agggcgccgc
     1741 cgaacagcat ggtgacttcg ctggggatcg ggatgcaggc cgattccgcc accatcagca
     1801 cgaacaccgc caggtagccg tactgcagga cttggtgggt gatcaggtgt tccacgcggt
     1861 ctcccgacgg tgacgggcat gactggcacg ttccaggcca ggatggcgca tcctacctcg
     1921 attcagcatg agcgccccgt gagcgttacc tgaatgttgg tggagggcgt gggcgctacc
     1981 ggctccagtg gctctcgccg agcaggtagg cggcggcgag gctctccagg cccgggtcga
     2041 gcggttcgcc ttcgccgggt tcggtcaccg agaccgagat ggtgcgggcg gcgtaccgcg
     2101 gcaggctggc aatccccgcg ccctccagct gaatcgcctc cgggtcggag gggtcggccg
     2161 tgacggtgta gctggcgcgc ggctccacgc cccgggagtg gaacagcttg agcaggtcgt
     2221 ggtcctcatt ctcgaagcgg aggatccgga caagactgcc ggcggtgaca ctggtcaggg
     2281 gcacaccagg caggcgcggg tagtcgccga tgggatggcc gtgggggcag gtcttggcct
     2341 cgccgatcat ggtcagcatg cgctgttcga ggctctcggt gacgcccatc tcgaaggtct
     2401 gggcctcctc gtggacctcg tcccaggaga cgcccatgac cttgaccagg aaggcctcga
     2461 ccagacggtg gcggctgacg acgtgcttgg cgacctgctc gccctcgtcg gtgagggtga
     2521 tctgcttgcc cttgccgggg ccacggcgga ccaggccgtc ccgttccagg cggcgcagca
     2581 tctcggtcac gctgggcggg gagacctgca tcgcccgtga gacgttggcg ggggtcggct
     2641 cgatccccgc ctccccgagc cagtagagcg actcgaggta ttcctgcacc gcttcggtca
     2701 tcgccatggt tgcatggtac cgggcggccg ggacgggatg cggggtcctg agggctcggc
     2761 taccatgcgc caccgtgcga ttccgacctg tttccatccg catcggcggg ctgccggccg
     2821 tcctggtcct gagcgccgcc ctggcccttg ccggcgcggg ctgcggctcc agcccgtcgc
     2881 aggacaaagc cgcgacgacc ccgccggtca gcggcgtcca cgacgtggag gcccgttcgc
     2941 tgcggttcag cccgccggcc atccaggtcc cggccgggac cacggtcagc tggcatttcg
     3001 acgacggcgc ggtgccccac aacgtcaccg gcaagggctt cgcctcgccg aacctcaagc
     3061 agaccacctg gagccaccgg ttcgccgccg ctggggcctt cacctaccgc tgcaccctgc
     3121 acgccaacat ggtcggcagg gtggtggtga ccgcccagtg agccctcgat gagcgagtcc
     3181 ccgcgcgagc ccttgagcga gcgcctgctc gcgctcgggg agccgtacct gcggcggcag
     3241 ctcgaccacc cgacggtgcg ggggatcggc gcgggcgacc tggacgagcc gcgcttccgc
     3301 gcctggctgg tgcaggacta ccgcttcctg ctcgactacg tccggctgtt cgcgctggcc
     3361 gcggcgcggg cgcccgacca ggaggcgctc agccggctgg tcgacctggc ccactcgacg
     3421 ctgcaccagg agctgtccct gcaccgctcc tacgcggccc agttcggcat cgccgaggcc
     3481 gacctggagc acgcccccaa gtcacgcacc tgcgccgcct acaccgactt cctgctgcgc
     3541 accgccgcca cggccgactt cgccgagacc gtcgccgcgc tcctgccgtg tctgtggggc
     3601 tactccgaga tcgggcgggc gctggccgcc gccggcgccc ccgccgagcc gcgctaccgc
     3661 cagtgggtgg aggcctacgc cgaccccgcc ttcgccgagc tggccgcctg gtgcgccacg
     3721 ctgctggacc gggtcaccga gggcgccggg gaggccaccc gcaagcgctg cgagcgcgcc
     3781 ttcgtcacca gcctggagca cgaaatggcc ttctgggacg tctgagcgag cgtcaggcgg
     3841 tcatgacgtc cccgcggatc gcccggaagg tcgcggtgtc ggccgggtcg gcgggcggct
     3901 cgaccaggtg gaaccagtag aacccgtggg gtccgatggc gatgcggtat ggggtcccgt
     3961 cgatgattgg gaagggcgtg cgcccgaaca tctcctcggg cagcaggccg atccagcggg
     4021 acaggtcgag ggtcaccggt tgggcgaagc gcgacaggtt ggcgaccacc aggacgacct
     4081 cgtcgccgag ctggcggatg aaggccatca cccggcggtt ctcggggtag agcggctcgt
     4141 agtcgccgat cccgaacacc gcgtgctgct tgcggacctg caccaggcgc cgcagccagt
     4201 gcagcagcga ggtcgccacc cgctcctggg cctcgacgtt gaccgcctgg tagccgtaga
     4261 ccgggtccat caggaacggc aggtagaggg cggccgggtc ggccttggag aagccggcgt
     4321 tgcggtcgcc ggtccactgc atcggcgtgc ggaccccgtt gcggtcgccg aggtagatgt
     4381 tgtcgcccat gccgacctcg tcgccgtagt acagcaccgg gctgcccggc aggctgagca
     4441 ggaggctgga gagcagctcg atctggcgcc ggccgttgtc gagcagcggc gccaggcggc
     4501 gcgcgatgcc gatgttgcgg cgcatgcgcg gctccttggc atactcggtg tacatgtagt
     4561 cgcgttcctc gtcggtgacc atctcaaggg tcagctcgtc gtggttgcgc aggaacaacc
     4621 cccactgcgc cgagtcgggg atcttggggg tgcggttcat gatctcgacg atcggggtgc
     4681 ggtcctcgcg gcgcagcgcc atgaacatgc gcggcatcac cgggaagttg aaggccatgt
     4741 ggcactcgtc gccgtcgccg aagtagggga ccacgtcggt cggccactgg ttggcctcgg
     4801 cgagcagcag cttgtcctcg tacttggagt cgacctcttt gcgcagccgt ttcaggaaca
     4861 cgtgggtttc gggcaggttc tcgcagttgg tcccctcccg ctcgaacagg taggggacgg
     4921 cgtcgaggcg gaagccgtcc aggcccaggt ccagccagaa gcggaccacc tccagcatcg
     4981 cctcctggac ctcggggttg tcgtagttga ggtccggctg gttggagaag aagcggtgcc
     5041 agtagaacgc ctgcgccacc ggatcccagg tccagttgga ggtctcggtg tccacgaaga
     5101 tgacccgggc gtcctggtac ttctggtcgg tatcggacca gacgtaccag tcgcgcctgg
     5161 cggagccggg ctggcgtgac tcctggaacc atgggtgctg gtcggaggtg tggttcatca
     5221 ccaggtcggc gatcacccgc atgccacgcc ggtgcgccgc ctcgagaaac tcgtagaaat
     5281 cggcgagtgt gccatactcg gggaggacgg agaaatagtc cgaaacgtcg taaccgtcgt
     5341 cccgcagcgg cgactggaag atgggcagca gccagatgca gtcgacgccg agccactgca
     5401 ggtagtcgag gcgctcggtg agcccgcgga agtcgccatg gccgtcggcg ttgccgtcct
     5461 ggaagccacg cacgtacaac tcatagaaga cggccgtttt gtaccaccgg gggtcgttca
     5521 acatcccgtc tgccagctcc tcagtcaagc aacctggacg gttcgcccag tcggccgacg
     5581 cggtgcgaag gtctcgccaa gtgacgccgt gggtaacgct gcgcatagtt ggcgttccat
     5641 gcaagcccgc atcgcaacca tgaaccgtca atgctcacgg tacgcatcaa gggaagcaag
     5701 atactgtccg cacttttggt gaaacgctcg gagaggagcg aggatctgag gatgagcacg
     5761 agtgcgcacg cccgcgacgc cgcggggggc agccgcttcc ggccccaccg gacaccccgg
     5821 ctggccgcgg cggtggtgct gctcgtgggc gtcatcgacc tgctctcggt gctgctgccc
     5881 gcccagcggg cccggttggc ggcgctgagc ggattcgtgc ccggggttgt cgaggcaggc
     5941 gcgagcgccg cgaccgccgc ggcggggatc gggctgatcc tgctggccgg cgggttgcgg
     6001 cggcgccggc gttcggcctt cgtggccacg gtggtcctcc tgctcggcag cgcgatcctg
     6061 catgtgatca agagtctcga cgtcgaggag gccctgattg aggcgtttct cggcggcctg
     6121 ctgctcggca gggccgaacg gttcagcgcc cgtgctggac ccggcgagcg tggcccggtt
     6181 gtccgcccgg ctgtggcgct ggtgctgatc acctacgggt acgggctgct cggcctggtg
     6241 atcaaccgcc gcaacgtgac cgggccgctg tcgttcggcc gggcggtcgt cgaggtcacc
     6301 aacatggcgc tcggcctcgg cacctccttg cacctgaccg gccggttcag ccgtttcttc
     6361 ccagcatcgg tgttcgtcct gttcatctcc ggggcggtga tcgtcctgct ccgggcgctt
     6421 gccccggtgc tctccagccg ctcgcgcagc cccgccctgc cggcgctggt cgcctcctcc
     6481 gacgactccc tggcctattt cgcgctgcgc gacgaccgcg ccaccatcgg ccctggcgac
     6541 gccatggtct cctacggcgc cgccagcacc gtggcgctgg cggccggcga cccgctcggc
     6601 ccgcgcgagc actggccagc cgccgtcgac gcgttcctcg ccgaggcggc cgaccagggc
     6661 cgggtcgccg ccgtgatcgc ctgcggcgcc gaggccatgg ccgtctacga gcaggccggc
     6721 ctgcgcaccg tctaccttgg cgacgaggcg gtgctgcgtc tcgacgagtt ctccctggag
     6781 ggccgggccg tacggatcgc gcggcagagc tggaaccgcg ccaagcgcgc cggctaccag
     6841 gccgaggtgg tgcgctccgg cgagctggac agcgccaccg gggcggccct gcaggcggtc
     6901 tcacggcact ggcgcggcga ggcggccgaa cgcggcttct ccatggcgct cggccgcctg
     6961 ttcgaccccc gcgacaagga caccctggtg gtgatggccc gcgacggcgg cggccgcatc
     7021 cgcggcttcc tgcatttcgt cccctgggag cgggacggcg tcagcctcga cgtgatgcgg
     7081 cgcgagcggg acgcaccggc gatcctcaac gatttcctgg tggtagaggc ggtccggcgg
     7141 ctgcccgagc tgggcgtgcg gcgcatgtcg ctgaacttct cgttcctgcg ggcggtcctg
     7201 gcggccggca gccagcccgg cgccccgctg ggcctgcggc tggagcgctg ggtgctgctg
     7261 cggctctcca agccgttcca gatcgagacg ctctaccgct tcaacaagaa gttcaaccca
     7321 tcctggcagc cccgctacgc ggcggtggag gctatcgagg acctgccgat ggtctccatg
     7381 gccgccctcc
//
LOCUS       DASWDC010000051         8108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1508218, whole genome shotgun sequence.
ACCESSION   DASWDC010000051 DASWDC010000000
VERSION     DASWDC010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508218"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..324)
                     /locus_tag="VF995_01595"
     CDS             complement(94..324)
                     /locus_tag="VF995_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9342295.1"
                     /translation="MSEGGYGYAVIIERGETSYGAYAPELPGVGVVGDSIEEVERLIR
                     EAIALHLRSLREHGEPIPEPSAVAATVIPATA"
     gene            complement(321..470)
                     /locus_tag="VF995_01600"
     CDS             complement(321..470)
                     /locus_tag="VF995_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9342296.1"
                     /translation="MLPVDGSYRQFRHPDKPGTVTVAGNPGTDLPPGTLASILRQAGL
                     RRPRA"
     gene            complement(528..3083)
                     /locus_tag="VF995_01605"
     CDS             complement(528..3083)
                     /locus_tag="VF995_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein"
                     /protein_id="HEX9342297.1"
                     /translation="MARRRFVLHVSLILALVMAAAVATATLGMAASSGSLGKLSPTLS
                     TSDRLDDRRFVAAGDRAYEVGTEAGRYPAMGFHTRGEMGGVWTAPLKLLDGLWFGVDG
                     SWVGPATTFTSGYGYAQFQLPGPAGVGITRTDFAPDGRRALEVGLTFDNSGGAKHLSL
                     ALDAHSELMSAYPWGATTPDQTAFNLPDQAAVAGGRLVFREQGTPPVANASAHDWAAL
                     VGSSLTPTASSTGSDFRGPQDPPVICPVSSEPDKSRCDDTAYGKGAGGQLDYALDLAP
                     HASETVWFTVAGSDHGLADATAQFTAASADPAGELAAKAASRLALAKQTQLTLPGDPQ
                     LAQGVEWGKQNLADLTQEAQDLSIRPTEAGTVYPAPVGHVAKIRFEGAGFPDYPWLFA
                     TDGEYTAFALAAAGQFDLIKDHLRALRDVSLIANHGSGKVVHEVVTDGSVYFGLNDEP
                     GDTDETAKFPGVVALVWRWTGDNAFRDEMYAFAKSNLEYLLTRVDVDHDLWPEGSGNV
                     EAPGLGQEKLDVTVYTIRGLFDLADMAASKHDSATQAWALSRANSMVKRFESTWYMPN
                     IPGYADSLVDPGNVKTQQRWWIGVTPMEVELFQGTTPVPGLAATASGNASLTLHESSC
                     YTGTFGLFVQGAAGCDPAPPPASPNRITFSLNTAIQSVGEGNYGRMGADQQQRYTTDN
                     RELMLPNPDEQPGDLPEIAPSPDFTRNIDRPFNERSMVEQAWGQYGLLWPAVHQQLGV
                     SPDLGMGLLEVVPQVPSGQATVSGSNILVGKGAVSVTATHSGTTWTTDVTASAAISAK
                     LTIGHTLPTGTKIASVTLNGSPVPFTTRTTNRGEEALVPATTGTLQHLVITTA"
     gene            complement(3245..4885)
                     /locus_tag="VF995_01610"
     CDS             complement(3245..4885)
                     /locus_tag="VF995_01610"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX9342298.1"
                     /translation="MRNRLWVLLVLVVVALSLLPMGLKVLAADSASTAATATFSNPIV
                     TNRSDPHIIKSGGQYYYTSTDGCPGAICVWQSATLTGLGSAARHVVWTIPACPAINCS
                     QVWAPEIHEIGGQFYIYYTASGGQIADHRLFALRSTDGTPTGTYAEAATGSAHGKLAE
                     SSGLLAIDPDVFTGADGKLYATWSGWPTSAGSEQDIYIAPMSDPLHLSGSRVSISAPT
                     RPWEVVGAKVNEGPVGFVHGGKTFISYSASGCWTSSYAVGLLTSSSGNLLDPASWSKS
                     GPHLKFHSGVSAPASFVPVQSLDDTETWFLVHSNTSACDPGRELRLQRLYWDPSGTPV
                     LGYPVADGVALPAPSGELGSTGTSPFTSGWGSAFGDAAEGNTTAGLRRGSWTTSSSTA
                     ASLTSFGGTTWTQLFRAANPNYETYTVSVQAKWSATGTTSKFPKYGIYASYDDRNNYV
                     AVFIDIKYKVLATYAVVQGVSAGWKNAALPSGFSGGSWHTLKVRKTGATYTFYLDGVQ
                     KQQRTFSGTFPVLLNGQAGLVTEDTKASYQQLAITSTS"
     gene            complement(5033..5533)
                     /locus_tag="VF995_01615"
     CDS             complement(5033..5533)
                     /locus_tag="VF995_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9342299.1"
                     /translation="MTAQERTNSKQQLATAVMAALIRLNKERLRENAERLRRYDLTTQ
                     QVWLLAELPEGEDQGVPIGSLAEALHCHGSNITGMVDRLEARGLVARQPSRTDRRVKF
                     VALTPSGSALRDKVIELARQPVRALLDQLSGDQLETLSELLDKACAGVAPLPRHHQPA
                     ERPREA"
     gene            5701..5898
                     /locus_tag="VF995_01620"
     CDS             5701..5898
                     /locus_tag="VF995_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342300.1"
                     /translation="MVLLHYRVVPGLGATPPPPPTPVPEQHAAPVSNPITLTGPAAAS
                     VTQVDVEEPCDCPGVTVAAAA"
     gene            complement(5928..6641)
                     /locus_tag="VF995_01625"
     CDS             complement(5928..6641)
                     /locus_tag="VF995_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342301.1"
                     /translation="MTSEPTEHLQRAHLRRHLPPALLAAANDRVHGASEIATRAIEGL
                     LEVAARGDPELLEAGAATLLAGQPAMAPVWHVAMAARQPDPVRALAALTRRLGEETEA
                     AARTAAAWLREHAAGPVRTVSESSLVGLTLRRLAEVATEPGKRADEAPVGLVGADAIG
                     PVELLNATGTAGLAAAMPTIVVATSIKLVPAAVFERLAAPGFERIPRERFAAVVLGAE
                     VVDPAEAGRRAAAIRTLGE"
     gene            complement(6678..7142)
                     /locus_tag="VF995_01630"
     CDS             complement(6678..7142)
                     /locus_tag="VF995_01630"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9342302.1"
                     /translation="MEAAKRPALSLGGIEIHVKMRAEETDGALSALEVVVDPGWLALP
                     HRHAWEDEVCRVLDGEVGLRVDDEELRAGAGAYAFLPRGMAHAYWNPGPAPARLQLLS
                     VPGGVERFYQELAGSPLGPLGAASAARRHGLVLVPEWIPDMVARFGLRVAGG"
     gene            complement(7246..7650)
                     /locus_tag="VF995_01635"
     CDS             complement(7246..7650)
                     /locus_tag="VF995_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9342303.1"
                     /translation="MIYLDACALVKFIKPEQETEALRAWRAALPKDTELLSSELALLE
                     ITRALLRGGADPTRVPYYVSQALRGVYVLELSGTVLDRARSYQVAKLGSLDAIHLATA
                     DPFRGELTRFITYDGELASAASGLGFPVAAPA"
     gene            complement(7647..7928)
                     /locus_tag="VF995_01640"
     CDS             complement(7647..7928)
                     /locus_tag="VF995_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9342304.1"
                     /translation="METIGLRELNQNPSRVVARVRAGVSIIVTDRGKPVLRMVPETEN
                     PSTLERMVSDGIAQPPAEQGMPDVISELADGVASLSDLVIADRAKERRR"
BASE COUNT         1145 a         2752 c         2950 g         1261 t
ORIGIN      
        1 ggagtccaaa caggtggtcc acaagaagcc tatgagaacc cctcgtgaac agccactcgg
       61 ccgaactggg aaccgtgcgc gatcgccccc ggctcaggct gtcgccggga tcaccgtggc
      121 ggcaacggcg gaaggctcag ggattggctc gccatgctcg cgaagcgagc ggaggtgcag
      181 cgcgatcgcc tcacggatca gccgctcgac ctcttcgatg ctgtcgccaa ccaccccaac
      241 gcccggcagt tcaggtgcgt atgcgccgta gctggtctcg ccgcgctcaa tgatgaccgc
      301 gtagccgtag ccgccttcgc tcatgccctc ggtctcctca gcccagcctg acgcagaatg
      361 ctggcgagcg tgccaggcgg caggtcagtg cccgggttac ctgcgactgt cacggtacca
      421 ggcttatccg gatgccggaa ttgtcgatag ctcccatcta cgggcaacac aacgcccccg
      481 aggtccgtgt gagaccagcg ggggcgttgg gtttgtctcg gctcaggtca tgcggtggtg
      541 atgaccaggt gctggagggt gccggtggtg gcggggacca gggcttcctc gccgcggttg
      601 gtggtgcggg tggtgaaggg gaccggggag ccgttgaggg tgacggaggc gatcttggtg
      661 ccggtgggga gggtgtggcc gatggtgagc ttggcgctga tcgccgcgct ggcggtcacg
      721 tcggtggtcc aagtggtgcc ggagtgggtg gcggtgacgc tgacggcgcc cttgccgacc
      781 aggatgttgc tgccggagac ggtggcctgg ccgctgggga cctgggggac gacctccagg
      841 aggcccatgc caaggtcggg actgacgccg agctgctggt ggacggccgg ccacagcagg
      901 ccgtactggc cccaggcctg ctcgaccatg gagcgttcgt tgaaggggcg gtcgatgttg
      961 cgggtgaagt ccggcgaggg ggcgatctcg ggcaggtcgc cgggctgctc gtcggggttg
     1021 gggagcatca gctcgcggtt gtcggtggtg tagcgctgct gctggtcggc gcccatgcgg
     1081 ccgtagttgc cctcgccgac cgactggatg gcggtgttga ggctgaaggt gatccggttg
     1141 gggctggccg gcggcggcgc cgggtcgcag ccagcggcgc cctggacgaa caggccgaag
     1201 gtgccggtgt agcaggagct ctcatgcagg gtcagggagg cgttgccgct ggcggtggcg
     1261 gccaggccgg gcaccggggt ggtgccctgg aacagctcga cctccatcgg ggtcacgccg
     1321 atccaccagc gctgctgggt cttgacgttg cccgggtcga ccagcgagtc ggcgtacccg
     1381 gggatgttcg gcatgtacca ggtcgactcg aaccgcttga ccatcgagtt cgcccgggag
     1441 agcgcccacg cctgggtggc gctgtcgtgc ttgctggcgg ccatgtcggc caggtcgaac
     1501 agcccgcgga tggtgtagac ggtgacgtcg agcttctcct ggccgaggcc gggcgcctcg
     1561 acgttgccgg acccctccgg ccacaggtcg tggtcgacgt ccaccctggt gagcagatat
     1621 tccagattcg acttggcgaa ggcgtacatc tcgtcccgga aggcgttgtc gccggtccag
     1681 cgccacacca gcgcgaccac gccggggaac ttggcggtct cgtcggtgtc gccgggctcg
     1741 tcgttgaggc cgaagtagac cgagccgtcg gtgaccacct cgtggacgac cttgccgctg
     1801 ccgtggttgg cgatcaggct cacgtcccgc aaggcccgca gatggtcctt gatcaggtcg
     1861 aactggcccg ccgccgccag cgcgaaggcg gtgtactccc cgtcggtggc gaacagccac
     1921 gggtagtcgg ggaagcccgc gccctcgaac cggatcttgg caacgtgccc caccggcgcc
     1981 ggatacaccg tcccggcctc ggtcgggcgg atcgacaggt cctgcgcctc ctgggtcagg
     2041 tcggcgaggt tctgcttgcc ccactcgacc ccctgcgcga gctgcgggtc gccggggagc
     2101 gtcagctggg tctgcttggc gagggcgagc cggcttgccg ccttggcggc cagctcgccg
     2161 gccgggtcgg cgctggccgc cgtgaactgc gcggtggcgt cggccaggcc gtggtcggag
     2221 cccgcgaccg tgaaccagac cgtctcgctg gcgtgcggcg ccaggtcgag ggcgtagtcg
     2281 agctggccgc cggcgccctt tccgtaggcg gtgtcgtcgc agcgggactt gtccggctcg
     2341 gagctgaccg ggcagatcac cggcgggtcc tgcgggccgc ggaagtcgct gccggtgctg
     2401 gaggcggtcg gggtcagcga gctgccgacc agcgcggccc agtcgtgggc gctggcgttg
     2461 gcgaccggcg gggtgccctg ctcgcgaaac accagccggc cgcccgcgac cgccgcctgg
     2521 tcgggcaggt tgaaggcggt ctgatcgggg gtggtcgccc cccacgggta ggccgacatc
     2581 agctccgagt gggcgtcgag cgccaggctc agatgcttcg cgccgccgct gttgtcaaag
     2641 gtgaggccga cctccagcgc ccggcggccg tccggcgcga agtcggtgcg ggtgatcccg
     2701 acccccgcgg ggccgggaag ctggaactgg gcgtagccgt agccgctggt gaaggtcgtg
     2761 gccgggccga cccagctgcc gtcgaccccg aaccacagcc cgtcgagcag cttcagcggc
     2821 gccgtccaca ccccgcccat ctcgccgcgg gtgtggaacc ccatcgccgg gtagcggcct
     2881 gcctcggtgc cgacctcgta ggcccggtcg cccgccgcca cgaagcggcg gtcgtcgagc
     2941 cggtcgctgg tcgacagcgt cgggctcagc ttcccgagcg agcccgatga ggccgccatg
     3001 cccagcgtgg ccgtggcgac ggcggcggcc atcaccagcg cgaggatgag ggatacgtgg
     3061 aggacgaatc gtcgacgcgc catcgcgttc tccttcttcg ccgtcacctg ggagcctgat
     3121 ccgctggagg ccgggcgccc gactcacccg cccggcggcc atttgcccgg aatgctcgga
     3181 cctaaaccaa gcgaatcgac ggagggacga cggagaaggc cctgagctga tggctcgggg
     3241 ctgctcacga ggtgctggtg atcgccagct gctggtagct cgccttggtg tcctcggtga
     3301 cgaggccggc ctggccgttg aggaggaccg ggaaggtgcc gctgaaggtg cgctgctgct
     3361 tctgcacgcc gtcgaggtag aacgtgtagg tggcgccggt cttgcggacc ttcagggtgt
     3421 gccaggagcc gccgctgaag ccggagggca gggcggcgtt cttccagccc gccgagacgc
     3481 cctgcacgac cgcgtaggtg gcgagcacct tgtacttgat gtcgatgaac acggcgacgt
     3541 agttgttgcg gtcgtcgtag gacgcgtaga tgccgtactt cgggaacttg gaggtcgtcc
     3601 cggtggcgct ccacttcgcc tgtaccgaga cggtgtaggt ctcgtagttc gggttggcgg
     3661 cccggaacag ctgcgtccag gtggtgcccc cgaacgaggt gaggctcgcg gcggtcgagc
     3721 tgctggtcgt ccacgacccg cggcgcaggc cggcggtggt gttgccctcg gcggcgtcgc
     3781 cgaaggcgct gccccagcca gaggtgaagg ggctggtgcc ggtggagccc agctctcctg
     3841 acggcgccgg cagcgcgacg ccgtcggcga cggggtagcc gagcaccggc gtgccgctgg
     3901 ggtcccagta gagccgctgc aggcgcagct ctcggccggg gtcgcaggcg ctggtgttgg
     3961 agtgcaccag gaaccacgtc tcggtgtcgt cgaggctctg gaccggtacg aacgacgcgg
     4021 gcgcgctgac gccgctgtgg aacttgagat gcgggccgct cttggaccac gaggccgggt
     4081 cgagcaggtt gccggaggag ctggtgagca ggcccaccgc gtagctgctg gtccagcagc
     4141 cgctggccga gtacgagatg aacgtcttgc cgccgtggac gaacccgacc gggccctcgt
     4201 tcaccttcgc cccgacgacc tcccagggcc gggtgggcgc cgagatgctc acgcgggagc
     4261 cgctgaggtg cagcgggtcg ctcatcggcg cgatatagat gtcctgctcg ctgccggcgc
     4321 tggtcggcca gccggaccag gtggcgtaca gcttcccgtc ggcgccggtg aagacgtcgg
     4381 ggtcgatcgc cagcagcccg ctggactccg ccagcttgcc gtgcgcggag ccggtggccg
     4441 cctcggcgta ggtgccggtc ggggtgccgt cggtcgaccg cagggcgaac agccggtggt
     4501 cggcgatctg cccgccggag gcggtgtagt agatgtagaa ctggccgccg atctcgtgga
     4561 tctcgggcgc ccagacctgg ctgcagttga tcgcggggca ggccgggatc gtccagacca
     4621 cgtgccgggc cgcggagccg agcccggtga gggtggcgga ctgccacacg cagatcgcgc
     4681 cggggcagcc gtcggtgctg gtgtagtagt actgcccgcc ggacttgatg atgtgcgggt
     4741 cggaccggtt ggtgacgatc gggttgctga aggtggccgt cgccgccgtg ctcgccgagt
     4801 ccgccgccag caccttcagg cccatcggca gcagcgagag cgccaccacc accagcacga
     4861 gcagtaccca cagccggttc cgcacccggt tcctccgtat cgcttgacgc tgttgtcgct
     4921 ggagccgttc atcgtcgccc gatgtcacag ccgttctgac gcgcgccaag ctagccgagt
     4981 aagcaaatcc aggacaaatt aagaaagaat cgtcccaaag gtgaggggcg gctcaggcct
     5041 cgcgcggtcg ttcggcgggc tggtggtggc gggggagggg ggcgacgccg gcgcaggcct
     5101 tgtcgagcag ctcgctgagg gtctcgagct ggtcgccgct gagctggtcg agcagggcgc
     5161 ggacgggttg gcgggcgagc tcgatgacct tgtcgcgcag cgcgctgccg gagggggtga
     5221 gggcgacgaa cttgacccgg cggtcggtgc gggagggctg gcgggcgacc aggccgcggg
     5281 cttccaggcg gtcgaccatg ccggtgatgt tggagccgtg gcagtgcagc gcctcggcca
     5341 gggagccgat cgggacgccc tggtcctcgc cctcgggcag ttcggcgagc agccagacct
     5401 gctgggtggt caggtcgtag cggcgcaggc gctcggcgtt ctcacgcagg cgctccttgt
     5461 tgaggcggat gagcgcggcc atcaccgcgg tggcgagctg ctgtttgctg ttggtccgtt
     5521 cctgggccgt catcgaatct cactgtccaa accactcggg gcaacgaccg ccgactcctc
     5581 atggtagcca ttgcctgagc tatcggcaac tgatgggtcg atagttgata tctcagatgt
     5641 ttatggttat catgatcttc aaccgaacgc caggagctca tcaagaaagg gggtcgcgtc
     5701 gtggtcttgc tgcactaccg cgtcgttccc ggcctgggcg ccaccccgcc cccgccgccg
     5761 acgccagtcc cagagcaaca cgccgccccg gtctccaacc ccatcaccct cactggtccc
     5821 gccgccgcct cggtcaccca ggtcgacgtc gaggaaccct gcgactgtcc cggcgtcacc
     5881 gtcgccgccg ccgcgtgacc gcggcagcgc cccggcgctg atccgcgcta ttccccgagc
     5941 gtcctgatcg ccgccgcccg ccgcccggcc tcggcgggat cgaccacctc cgcgccgagc
     6001 accaccgcag cgaaccgctc gcgtgggatc cgttcgaagc cgggcgcggc caggcgctcg
     6061 aacaccgccg ctggaaccag cttgatcgag gtggccacca cgatcgtcgg catggccgcc
     6121 gccagtcccg ccgtgccggt cgcgttcagc agctctaccg gaccgatcgc gtcggcgccg
     6181 acgagcccaa ccggggcctc gtccgcccgc ttcccaggtt ccgtcgccac ctccgcgagc
     6241 cgccgaaggg tcagcccgac cagcgagctc tcggaaacgg tccggaccgg tccggcggcg
     6301 tgctcccgca gccacgccgc cgcggtccgg gcggccgcct cggtctcctc gccgagtcgc
     6361 cgggtcaggg cagccagtgc cctgaccggg tcaggctgcc gcgcggccat ggcgacgtgc
     6421 cacaccggcg ccatcgccgg ctgaccggcg agcagcgtcg ccgccccggc ctccagcagc
     6481 tccggatcgc cacgcgctgc cacctccaga agtccctcga tggcccgcgt ggcgatctcg
     6541 ctggcgccgt ggacacggtc gttggcggcc gccagcagcg ccggtggcag gtgtcgccgg
     6601 aggtgcgctc gctgtaggtg ttctgttggt tcgctggtca tgccgcgaag cctaccgaag
     6661 gtcgccagcc tcctcggcta tcccccggca actcggagcc cgaaccgggc caccatgtcc
     6721 gggatccatt caggaaccag caccagcccg tgccgccgcg ccgccgaggc ggcccccagc
     6781 ggccccagcg ggctgcccgc cagttcctgg tagaaccgct ccaccccgcc cggcacgctc
     6841 agcaactgca accgcgccgg cgcgggcccc gggttccagt aggcatgcgc catcccacgc
     6901 ggcaggaacg cgtacgcccc agcgccggcc cgcagctcct cgtcgtccac ccgcaatccg
     6961 acctcgccgt ccagcacccg gcacacctcg tcctcccagg catgccggtg cggcaacgcc
     7021 agccaccccg ggtcgaccac cacctccagc gccgacagcg ccccatccgt ctcctcggcg
     7081 cgcatcttga cgtggatctc gatcccaccc agcgacaacg ccggacgctt cgccgcttcc
     7141 atccccggcg cgccccttcc cacgaaccga gcctctgacc cgcctctaat gtcgcatgcg
     7201 ccttatcctg cggctatccc cacgctgggc cgcctgatga ctggtctatg cgggtgctgc
     7261 gactggaaag ccgaggccgc tcgctgccga cgcgagctcg ccgtcgtagg tgatgaagcg
     7321 ggtgagctca ccgcggaacg ggtcggctgt agcgaggtgg atcgcgtcga gcgagccgag
     7381 cttcgccact tgataggacc gggcccggtc cagaacggtg cccgaaagct cgagtacata
     7441 gacgccccgc aaggcttggc tcacgtagta gggcacccgg gtggggtccg caccgccccg
     7501 gaggagtgcc cgcgtgatct ccagcagcgc cagttcgctg gaaagcagct cggtgtcctt
     7561 cgggagcgcc gctcgccaag ctcgcagtgc ctcggtctcc tgctcgggtt tgatgaactt
     7621 caccaacgcg caggcgtcga ggtagatcac cgacggcgtt ccttggcacg gtcagcgatc
     7681 accaggtcgg acaagctggc aacgccgtcg gccaattcgg agatgacgtc aggcatgccc
     7741 tgctcggccg gcggctgcgc gatgccgtcg ctgaccatgc gctcaagcgt gctggggttt
     7801 tcggtctcgg gcaccatccg gagcacgggc ttgccgcgat cggtgacgat gatcgacacc
     7861 ccagctcgca cccgtgcgac caccctcgaa gggttctggt tcagctctcg caagccgatg
     7921 gtctccatcc ccggagtgta cctacaaaag gtaggcacat caaccagacc gctacgccgc
     7981 cagcggcagg gtgagcagga ccgtcgggac caggaggagc gcggcggcga gcaggtaggc
     8041 ggcggggggg acccaggcgg ggacgggggt gggtgggtcg agcagacggc ggacgcgggc
     8101 gagggtgg
//
LOCUS       DASWDC010000052         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_10907, whole genome shotgun sequence.
ACCESSION   DASWDC010000052 DASWDC010000000
VERSION     DASWDC010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10907"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1554
                     /locus_tag="VF995_01645"
     CDS             <1..>1554
                     /locus_tag="VF995_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9342305.1"
                     /translation="CRRARERGERGVKALLLYPMNALATDQARRIGELVASDPALRPV
                     TAGLYIGGDGAHDRMGSDHIIDQRDVITASPPDILLTNYKMLDFLLLREHDAALWRAS
                     RATLRYVVLDEFHTYDGAQGTDVAMLLRRLGATLHADEPGRPLGRICPVGTSATLGDG
                     TDPAAMRAFAERVFGTPFEADAVIGEDRLSAPEVVTAIDYELPVPDVEQVLAARDPVA
                     DDPASWAELAEVFTGQPETDPLALGELLRRHFMTRAVVEALGGDPLTAAEAVEAIADK
                     GVLSWGVHQRKAPGRVELALLRWVALLSYARRPGGKEPETRPLLTVEVQLWIRELRRM
                     LRRVGSEPGFSWWHDGPAEDKARSLPATYCRNCGRSGWTALAPELDGPLGGNPEEIWR
                     ESLTNPARLRTLIAAGADEPEVTWLDSATLERHASHTDGDGRLPVLVTTRQEAAKDEL
                     CPSCGADDSIRFLGSSIATLVSVALTQMFGSDLLREPEKKTLVFTDSVQDAAHRAAFI
                     EGRAFQFNLR"
BASE COUNT          217 a          618 c          525 g          194 t
ORIGIN      
        1 tgccggcggg cgcgggagcg gggggagcgg ggggtcaagg cgctgctgct gtacccgatg
       61 aacgcgctcg ccaccgacca ggcgcggcgc atcggcgagc tggtcgcgag cgaccccgcc
      121 ctccgcccgg tcacggccgg gctctacatc ggcggcgacg gcgcccacga ccgcatgggc
      181 agcgaccaca tcatcgacca gcgcgacgtg atcacagcct ccccgcccga catcctgctg
      241 acgaactaca agatgctcga cttcctgctg ctccgcgagc atgacgcggc cctgtggcgg
      301 gcctcccgcg cgacgctgcg ctacgtcgtg ctggacgagt tccacaccta cgacggcgcc
      361 cagggcaccg acgtcgcgat gctgctgcgg cggcttgggg ccaccctcca cgccgacgaa
      421 cccggccgcc ccctcggcag gatctgcccg gtcgggacct cggcgacgct cggggacggc
      481 accgacccgg cggcgatgcg cgcctttgcc gaacgggtct tcggcacccc gttcgaagcg
      541 gacgcggtca tcggcgagga ccgcctcagc gcccccgagg tcgtcaccgc gatcgactac
      601 gagctgccgg tccccgacgt cgaacaggtc ctcgccgccc gcgacccggt cgccgacgac
      661 cccgcgagct gggccgagct cgccgaggtg ttcaccggcc agccagagac cgacccgctg
      721 gccctcggcg agctgctgcg gcgccacttc atgacccggg ctgtcgtgga ggcgctcggc
      781 ggcgacccgc tcaccgccgc cgaggcggtc gaggcgatcg ccgacaaggg cgtgctgtcg
      841 tggggagtcc accagcgcaa ggcccccggc cgggtcgagc tggcgctgct gcgctgggtc
      901 gccctgctct cctacgcccg ccgccccggc ggcaaggaac ccgagacccg gccgctgctc
      961 accgtcgagg tgcagctctg gatccgggag ctgcggcgga tgctgcgacg ggtcggcagc
     1021 gagccgggtt tctcctggtg gcacgacggc ccggccgagg acaaggcccg gtcgctgccg
     1081 gccacctact gccgcaactg cggccgttcc ggctggaccg ccctcgcccc ggagctggac
     1141 ggcccgctcg gcggcaaccc ggaggagatc tggcgcgaga gcctcaccaa tcccgcacgc
     1201 ctccgcacgc tgatcgccgc tggcgcagac gagcccgagg tcacctggct cgactccgcc
     1261 accctggagc gccacgccag ccacaccgac ggcgacgggc gcctcccggt gctggtaacc
     1321 acccgccagg aggcggccaa ggacgagctg tgcccctcct gcggcgccga cgacagcatc
     1381 cgcttcctcg gctccagcat cgccaccctc gttagcgtcg ccctcaccca gatgttcggc
     1441 tccgacctgc tgcgcgagcc cgagaagaag accctggtgt tcaccgactc ggtccaggac
     1501 gccgcccacc gcgccgcctt catcgagggc agggccttcc agttcaacct ccgc
//
LOCUS       DASWDC010000053         7128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1428540, whole genome shotgun sequence.
ACCESSION   DASWDC010000053 DASWDC010000000
VERSION     DASWDC010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428540"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..2137
                     /locus_tag="VF995_01650"
     CDS             173..2137
                     /locus_tag="VF995_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEX9342306.1"
                     /translation="MRADHSQTTASRRRFARSSALAATMTLTMLFAAGCGAGPSLARG
                     SGTAFIRVNQVGYVGAASKSAYLMAGGSEAGATFSVRDAAGKPVFEARIGANLGRWSS
                     RYPDVYALDFSSVRTAGSYTVTVRGPMAASSPRFRIDTGAHLYAGAIANAVAFYQAER
                     DGPQFVAGPLRSAPGHLHDRTAMTYLTPKVNQDGDFSGDLRPLGVRIDASGGWWDAGD
                     YLKFVETTSYTVDVQLLGVRDFPTQLGPGFAREARFGTDWLRRMWDDHTRTLYYQVGI
                     GEGNARIVGDHDIWRLPQADDHFGGNDPADRYIRNRPVFRAGAPAAPISPNLAGRDAA
                     AFALCYQVFRGSDPAYANRCLLEGEHIFDLADTAPRGHLLTVIPFDFYPETEWRDDLE
                     LGAAELAQALQAATPPAGLPLPHRDPRFYLRQGAHWADAYIHGPNDAADTLNLYDSSA
                     LAHADLSRAIARAGDPAGLEVTRAALLADLRKAIGGALAKAGADPFQFGFPWASYDTQ
                     THGTGLSLMASLYDQLSGTSAFADWSGRWLSGVLGANAWGASFIVGDGTTFPHCMQHQ
                     VANLVGSLDGSPPLLRGAGVEGPNSFAATGRVSGMRTCPPSGGDRFARWSGGGAVFRD
                     DVQSFSTTEPAIDLTVNSPLAFARQAAGLR"
     gene            complement(2182..3186)
                     /locus_tag="VF995_01655"
     CDS             complement(2182..3186)
                     /locus_tag="VF995_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9342307.1"
                     /translation="MTPLRIGIIGTGNMGQEHARRLAGVPGAALVAVADVNVEQAKRV
                     AEAAGARALADSHELIGSDAVEAVLIASPSDTHEEFTQACIAAGKPMFCEKPLAPTAD
                     ACLRVLAAETAQPRRLVQVGFMRRYDDDFRALKQTLDSGQLGRALLLHCRHRNPDSLP
                     GFTSDMMITDSMVHDIDMTRWLLGQEVTAASVFQPRSSSLAPEGLLDPQLVLLETAEG
                     VVVDVESFVNCQYGYDIRYELVGETGTASLGETAGVHVRQRGRHHGRVPASYQERFGS
                     AYQRELRAWVAGACSGELTGPSTWDGYATTAVAEACVQALHEGCRATVALADRPAFYA
                     "
     gene            complement(3183..4139)
                     /locus_tag="VF995_01660"
     CDS             complement(3183..4139)
                     /locus_tag="VF995_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX9342308.1"
                     /translation="MTDNPPIPTRSRLRLGSCPDSWGVWFADDPAQTPWPRFLDELAQ
                     AGYRWLELGPYGYLPTDPARLRDEVDRRGLRVSGQAVFGALHRAERWQSDIEAARRVA
                     ALVTAMGARHVILLPAAFRDLDGTLTEPRELDRDGWCTLVEAASRLGRILHEEYGVTA
                     AFHPHADSHVGTQAQVERFLHDTDPASVSLCLDTGHIAYYRGDNLELIRAYPDRIAYL
                     HLKQADPAVVAQVEAEDLCFAAAARRGVMCEPPQGLPEVESLLDAVDRHLGLGGLGRE
                     LYMIVEQDLYPCDPGDPLPIATRTRKYLRQLGVGSEREGGAR"
     gene            4399..5133
                     /locus_tag="VF995_01665"
     CDS             4399..5133
                     /locus_tag="VF995_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342309.1"
                     /translation="MGTPPPRHLAHPQGRSGLSRRQRATAGVALAVTVAVALAAVALT
                     RVPGSGPARHASPRSRPGAAAGTAPGTGDTFPPMVAMPGNLLDNGDVEGGLSGWSPLG
                     GAALARRSPGHSGRWALAFGVPGSTTSPAPAPGAAGAAPGVRFADVTTSQAGRTYQAT
                     AWVRADRPGTTVAFALRELPAQRSAPASADVLSLTLPADGSWRQIAVVHQARMPGARV
                     VLDLAATRLPGGQVIADQLDVEISQS"
     gene            5294..5827
                     /locus_tag="VF995_01670"
     CDS             5294..5827
                     /locus_tag="VF995_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342310.1"
                     /translation="MGRTRLLVHGLSGPLGLLALPAAILLLGLALGLAGSGSAAAQTS
                     LGLRGVSCDRLTVSGEGLSPSTRFTLTILNPADLRALAQRTVTTSAAGTLATRVSVSL
                     SGLRAVRAALRRPGAAPIWADQSLPAPCPLASTGGGHTAPLLAFAVAALAGGVVLVNA
                     YAYHGRHLVAAGRHARS"
     gene            complement(5855..6364)
                     /locus_tag="VF995_01675"
     CDS             complement(5855..6364)
                     /locus_tag="VF995_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019738620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9342311.1"
                     /translation="MTGRSAGILLYRITTHPPAGAAEADLEVLLVHPGGPFWARKDLG
                     AWSIPKGEYGDGDDPLQAALREFEEETGRRPPGERLLALGTVRQRGGKQVTAWAAAAG
                     ELDLDAAQLRSNTFTLEWPPRSGQLREFPEVDRAGWFDLPTARAKLVGAQAELLDRLV
                     AALGSGQDP"
     gene            complement(6435..>7128)
                     /locus_tag="VF995_01680"
     CDS             complement(6435..>7128)
                     /locus_tag="VF995_01680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020138350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9342312.1"
                     /translation="RSFHAAGAAVVVLDRRPEALAELPGGIGYVAADISDDAAVRAAV
                     AAAAAALGGLDVLVNNAGIGAQGSVEQGRDEEWQRVLDVNVVGTARVSRAVWPHLRES
                     KHAAVVNMASIAATVGLPQRAVYSASKGAVLALTRAMAADGMPDGIRVNAVSPGTADT
                     PWVGRLLDRAPDPAAERAALEARQPHGRLVSAEEVARAALYLASPLAGSTTGAELAVD
                     GGMAGLRLRPRS"
BASE COUNT          923 a         2768 c         2473 g          964 t
ORIGIN      
        1 gcacgcccca acctcaatcg cgcaccaagt cgtaacttct tgcggtgagt gagctggtat
       61 ccccgaagct gcttgcgtca attgtccggc catgccgaaa agagtaaaat agcttattgt
      121 tagagaacct tactaatcgc cgtcagggat cgccgaagga ggcgtggcag cgatgcgcgc
      181 ggaccactcc cagaccactg cctccaggcg gcgcttcgcc cgctcctccg ccctcgccgc
      241 aaccatgacc ctgaccatgc tcttcgccgc cggctgcggc gcggggccct cgctggccag
      301 ggggagcggc accgccttca tccgcgtcaa ccaggtcggc tacgtcggcg ccgccagcaa
      361 gagcgcctac ctgatggccg gcggcagcga ggcgggcgcc accttcagcg tccgggacgc
      421 cgccggcaag ccggtgttcg aagcccggat cggcgccaac ctggggcgct ggagcagccg
      481 ctaccccgac gtctacgccc tcgacttcag ctcggtcagg accgccggca gctacacggt
      541 cacggtgcgc ggcccgatgg ccgcctcgtc gccccgcttc cgcatcgaca ccggcgcgca
      601 cctgtacgcc ggcgcgatcg ccaacgccgt cgccttctac caggccgaac gggacggccc
      661 gcagttcgtc gccgggccgc tgcgcagcgc ccccgggcac ctgcacgacc gcaccgccat
      721 gacctacctg acgccgaagg tcaaccagga cggcgacttc agcggcgacc tccgcccgct
      781 cggggtgcgg atcgacgcct ccggcggctg gtgggacgcg ggcgactacc tgaagttcgt
      841 ggagaccacc agctacaccg ttgacgtgca gctcctcggc gtgcgcgact tccccaccca
      901 gctcggcccc ggcttcgccc gcgaggccag gttcggcacc gactggctgc ggcgcatgtg
      961 ggacgaccac acccgcacgc tctactacca ggtcggcatc ggcgagggga acgccaggat
     1021 cgtcggtgac cacgacatct ggcggctgcc ccaggccgac gaccacttcg gcggcaacga
     1081 cccggccgac cgctacatcc gtaaccggcc ggtgttccgt gccggcgccc ccgcggcgcc
     1141 gatcagcccc aacctcgctg gccgcgacgc cgccgcgttc gccctgtgct accaggtctt
     1201 ccgcggcagc gacccggcat acgccaaccg ctgcctgctg gagggcgagc atatcttcga
     1261 cctggccgac accgcccccc gcggccacct gctcacggtg atcccgttcg acttctaccc
     1321 cgagaccgaa tggcgcgacg acctggagct cggcgccgcc gagctggccc aggcgctgca
     1381 ggccgccacc ccgccggccg ggctccccct cccccaccgc gacccgcggt tctacctgcg
     1441 gcagggcgcc cactgggcgg acgcctacat ccacgggccg aacgacgcgg ccgacaccct
     1501 caacctctac gacagcagcg ccctcgccca tgccgacctg tcccgggcga tcgcccgggc
     1561 cggagacccg gcgggcctgg aggtcacccg cgccgcgctg ctcgccgacc tcaggaaggc
     1621 gatcggcggc gccctcgcca aggccggcgc cgaccccttc cagttcggct tcccctgggc
     1681 cagctacgac acccagacgc acggcaccgg cctgtcgctg atggccagcc tctacgacca
     1741 gctcagcggg accagcgcct tcgctgactg gagcggccgc tggctcagcg gcgtccttgg
     1801 cgccaacgcc tggggtgcct cgttcatcgt cggcgacggc accaccttcc cgcactgcat
     1861 gcagcaccag gtcgccaacc tggtcggctc gctggacggc tcgccgccgc tgctgcgcgg
     1921 cgcgggcgtg gagggcccga actcgttcgc ggccaccggg cgggtcagcg gcatgcggac
     1981 ctgcccgccg tccggcgggg accgcttcgc gcggtggagc ggcggcggcg ccgtcttcag
     2041 ggacgacgtg cagtcgttct cgaccaccga gccggcgatc gacctcaccg tcaacagccc
     2101 gctggccttc gcccgccagg ccgccggcct gcggtaggcg gagtgctccg ccagggcctg
     2161 gctccgggct ccccttcccg gtcaggcgta gaacgctggc cgatcggcca gcgcgacggt
     2221 ggcgcggcag ccctcgtgga gggcctggac gcaggcctcg gccacggcgg tggtggcgta
     2281 gccgtcccag gtgctcgggc cggtcagctc gccgctgcac gcgcccgcga cccaggcgcg
     2341 cagctcgcgc tggtaggcgc tgccgaatcg ttcctggtag ctggccggga cgcggccgtg
     2401 gtggcggccc cgctgcctga cgtgcacgcc ggcggtctcg ccaagcgagg cggttccggt
     2461 ctccccgacc agctcgtagc ggatgtcgta gccgtactgg cagttgacga agctctccac
     2521 gtcgaccacc acaccctccg cggtctccag caggacgagc tgcgggtcca gcagcccttc
     2581 cggggccagg gagctggagc gcggctggaa gacgctggcg gcggtgacct cctggccgag
     2641 cagccaccgg gtcatgtcga tgtcgtggac catcgagtcg gtgatcatca tgtcgctggt
     2701 gaagccgggc aggctgtcgg ggttgcggtg gcggcagtgc agcagcagcg cccggccgag
     2761 ctgcccgctg tcaagcgtct gcttcagagc gcggaagtcg tcgtcgtagc ggcgcatgaa
     2821 cccgacctgc accagccgcc gcggctgggc ggtctccgcc gccagcaccc gcaggcaggc
     2881 gtcggcggtg ggggccagcg gcttctcgca gaacatcggc ttgcccgcgg cgatgcaggc
     2941 ctgggtgaac tcctcgtggg tgtcgctcgg cgaggcgatc aggacggcct cgaccgcgtc
     3001 gctgccgatc agctcgtggc tgtcggcgag ggcgcgcgcc ccggccgctt cggcgacccg
     3061 tttggcctgc tcgacgttga cgtcggcgac cgcgaccagc gcggcgccgg ggacgccggc
     3121 gagccggcga gcgtgctcct ggcccatgtt cccggtgccg atgatcccga tcctgagcgg
     3181 cgtcaccgcg cacccccctc gcgctcgctc cccacgccga gctgccggag gtacttgcgg
     3241 gtgcgcgtcg cgatcgggag cgggtcgcct gggtcgcagg gatacaggtc ctgctccacg
     3301 atcatgtaca gctccctgcc gaggccgccg aggccaagat ggcggtcgac cgcgtcgagc
     3361 agcgactcga cctcgggaag gccctgcggc ggctcgcaca tgacacccct gcgggccgcc
     3421 gccgcgaagc agaggtcctc ggcctcgacc tgcgcgacga cggccgggtc ggcctgcttc
     3481 aggtggaggt aggcgatccg gtcggggtaa gcacggatca actccaggtt gtcgccccgg
     3541 tagtaggcga tgtggccggt gtcgaggcag agcgagacgc tggcggggtc agtgtcgtgg
     3601 aggaagcgct cgacctgcgc ctgggtcccg acgtggctgt cggcgtgggg atggaacgcg
     3661 gcggtgacgc cgtactcctc gtggaggatc cggccgagcc gggaggcggc ctcgaccagc
     3721 gtgcaccagc cgtcgcggtc cagctcacgc ggctcggtga gggtgccgtc caggtcgcgg
     3781 aaggcggcgg gcagcaggat gacgtgcctg gcacccatcg cggtgaccag cgcggccacg
     3841 cgccgtgcgg cctcgatgtc gctctgccac cgctcggcgc ggtgcagggc cccgaacacg
     3901 gcctggccgg agacccggag gccgcgacgg tcgacctcgt cgcggagcct ggccgggtcg
     3961 gtcggcaggt agccgtaggg gcccagctcc agccaccggt agccggcctg ggccagctcg
     4021 tcgaggaacc gcggccaggg cgtctgggcc gggtcgtcgg cgaaccagac gccccacgag
     4081 tcggggcagc ttcccagccg aaggcgggac cgggtcggga tcggtgggtt gtccgtcatg
     4141 ggcagcggtg cacctcctca ccctcacgca tcctcaacgg ccgccggact gctcggcgct
     4201 ggacgtcctg cggagcccgc gccgtctgct ggcggccacg gcagggcgtg gcggcggtcg
     4261 cttatacgta ctgcgcactt aagttactgt caagagtttg tattgacgta ctgacaacgt
     4321 aaccggcttg ccgacccaac cggcggcgcg ggcgcgaggt ttacccctcc caccggctgc
     4381 caagaactcc cgcagaccat gggcactccc ccgccacgcc atctcgccca cccacagggc
     4441 cgctccgggc tgtcacgccg gcagcgcgcg accgccggcg tcgcgctggc cgtgaccgtc
     4501 gccgtcgcgc tcgccgcggt ggcgctgacc cgcgttccgg gctcgggccc ggcccggcac
     4561 gccagccctc gatcacgacc cggcgctgcc gccggcaccg cccccggcac cggcgacacg
     4621 ttcccgccga tggtggcgat gccgggcaac ctgctcgaca acggcgacgt ggagggcggg
     4681 ctcagcggct ggtcaccgct tggcggcgcc gccctggccc gtcggagccc cggccattcg
     4741 ggccgctggg cgctcgcctt cggcgtcccc ggaagcacca cctccccggc cccggcgcct
     4801 ggcgccgccg gggcagcgcc gggggtgcgg ttcgccgacg tcaccaccag ccaagcgggc
     4861 cgcacctacc aggccaccgc ctgggtgcgc gccgaccggc cgggcaccac cgtggccttc
     4921 gccctccgcg agctcccagc ccagcgcagc gctccagcca gcgccgacgt cctcagcctg
     4981 acccttcccg ccgacggttc ctggcggcag atcgcagtcg tccaccaggc ccgcatgccc
     5041 ggcgccaggg tcgtcctgga cctggccgcg acccgcctgc ccggcggcca ggtcatcgca
     5101 gaccagctcg acgtcgagat cagccagagt tgactagccc gaacggcgat tccggccggt
     5161 tctgtgggag cgctcctcag tagtgctgat catcgggcgc tgcttgttta cctgccggtg
     5221 ccggagtgca acctctcaac gcggaacgat caatccggcc acgaacgcac cgcgatccgc
     5281 agcgggaggc cccatggggc gcacacggct cctcgtgcat ggactgtctg gaccgctcgg
     5341 cctgctcgcg ctccccgcag ccatacttct cctgggtctg gcgctgggtc tggccggcag
     5401 cggcagcgcc gccgcccaga cctcgctcgg tctgcgcggt gtcagctgcg accgcctgac
     5461 cgtcagcggc gaaggcctgt cgccctccac tcgcttcacc ctcacgatcc tcaaccccgc
     5521 cgaccttcgt gccctcgccc agcgcaccgt gaccaccagc gccgccggca ccctcgccac
     5581 gcgggtcagc gtctccctca gcggcctccg cgccgtccgc gccgccctgc gccgcccggg
     5641 cgccgccccc atctgggccg atcagtccct cccggctccc tgccccctgg ccagcaccgg
     5701 cggcggccac accgccccgc tgctcgcctt cgccgtcgcc gccctcgccg gcggggtcgt
     5761 gctggtgaac gcctacgcct accatggccg ccacctcgtc gctgccgggc ggcacgcccg
     5821 ctcgtgatcc ggcacggggc aaccctcgcg ggggctacgg gtcctggccg gagccgaggg
     5881 cggcgaccag ccggtcgagc agctcggcct gcgcgccgac cagcttggcg cgagcggtcg
     5941 gcaggtcgaa ccatccggcg cggtccacct cggggaattc ccggagctgc ccggagcgcg
     6001 gcggccactc cagcgtgaag gtgttgctgc gcaactgggc agcgtccagg tccagctcgc
     6061 cggctgctgc cgcccaggcc gtgacctgct tgccgccgcg ctggcggacc gtgcccaacg
     6121 ccagcagacg ctcaccgggc gggcgccggc cggtctcctc ctcgaactcc cgcagggcgg
     6181 cctgcagcgg gtcgtcgccg tccccgtact cgcccttggg aatcgaccag gcgcccaggt
     6241 ccttgcgcgc ccaaaagggc cccccaggat gcacgagtag gacctccaga tcagcctcag
     6301 ccgcccctgc cggcgggtgg gtggtgatgc ggtagagcag aatgcccgca ctgcgcccgg
     6361 tcatgtccgc agcccctcca ggccgtgttc cccgactggc gttgctgtcc agggccattt
     6421 gctgctcacg agtctcatga ccgggggcgg agccggaggc cggccatgcc gccgtcgacc
     6481 gcgagctcgg cgccggtggt cgagccggcg aggggtgagg cgaggtagag cgcggcgcgc
     6541 gcgacctcct cggcgctgac caggcggccg tggggctgcc gggcctccag ggcggcgcgc
     6601 tcggcggcag ggtcgggggc gcggtcgagc agccggccga cccacggggt gtcggcggtg
     6661 ccggggctca cggcgttgac gcggatgccg tcgggcatgc cgtcggccgc catcgcgcgg
     6721 gtcagcgcca gcaccgcgcc cttgctcgcc gagtagaccg cccgctgcgg cagcccgacg
     6781 gtggcggcga tcgaggccat gttgaccacc gccgcgtgtt tcgactcgcg caggtgcggc
     6841 cagaccgccc gcgacacccg cgccgtgccg acgacgttga cgtcgagcac ccgctgccac
     6901 tcctcgtcgc ggccctgctc gaccgatccc tgcgctccga tgccggcgtt gttgaccagc
     6961 acgtccagcc cgccgagcgc ggcagcagcc gccgcgaccg cggcccgcac cgcggcgtcg
     7021 tcgctgatgt cggcggccac gtagccgatc ccgccgggca gctccgccag cgcctccgga
     7081 cggcggtcga gcacgaccac ggccgcgccc gcggcgtgga agctgcgc
//
LOCUS       DASWDC010000054        25036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1180026, whole genome shotgun sequence.
ACCESSION   DASWDC010000054 DASWDC010000000
VERSION     DASWDC010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 25036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180026"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VF995_01685"
     CDS             complement(<1..211)
                     /locus_tag="VF995_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9342313.1"
                     /translation="MPMKSGAIYARISQVLDPEDTLGVRRQIEDCQHGADRRGDRIAH
                     VYTDNDVSATGDKPRPAYQRMLKAIH"
     gene            242..823
                     /locus_tag="VF995_01690"
     CDS             242..823
                     /locus_tag="VF995_01690"
                     /inference="COORDINATES: protein motif:HMM:NF025888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342314.1"
                     /translation="MAQHGLHVNEDHFLVEVIDPTTGSRVAAGERGELVFTTLTKEAM
                     PLLRYRTGDIATLDRLPCPCGRTLVRMSKVLGRVDDMLVVRGVNVYPSEVEAVLLAEP
                     GVGAQYLLVVDRTRTLAALEVVCELAEAASGGGDQAHQAEGVRAAVALALERRLGLRP
                     EVRVLAAGTLPRTEVGKARRVVERSAEHDPLTL"
     gene            960..1532
                     /locus_tag="VF995_01695"
     CDS             960..1532
                     /locus_tag="VF995_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX9342315.1"
                     /translation="MGKVVVTEFISLDGVMEDPGGAEKDRYERGGWTFQFDRGEEGDK
                     FKFDELMASDAGLLGRVTYEGFAKAWPAMDTDDFGRKLNSMPKFVVSSTLENPTWKNT
                     TVLRGDLAEEVGKLKEQFAGDIMVAGSGRLAQSLLARDLVDRLHLMVFPVLLGEGKRL
                     FADGTDLTRWQLVETKQTGEVVILVLERER"
     gene            1623..3671
                     /locus_tag="VF995_01700"
     CDS             1623..3671
                     /locus_tag="VF995_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HEX9342316.1"
                     /translation="MNPKRLTHDLNPEQRAAVEACSGPVCILAGAGTGKTRVISRRVA
                     YAIATGAVDPRHVLVVTFTEKAAGEMRERLAGLGFPGVQAQTFHAAAFRQLRYFWPRL
                     SQEPLPEVADSKAVLLVPLQRSLPGGYKFTALKDLADEIEWAKARRLDPDGYAAAVVR
                     LGRTPPVPADLFTGLFRHYEWAKERAGRIDFEDMLVRMLDALERREDIAEEFRGQYRW
                     FSVDEYQDTNPLQQALLDAWLGGRDDLAVVGDEDQTIYTFTGATSDYLTGFKRRYPEA
                     CVFRLEQNYRSTPEVLAVANRLLTRSGGSPGKRLVATRASGPEPVIAAFESAEGEAAA
                     LVAEVGRLGALGVALEEVAVLLRTNAQIPLVEEALAAAGIRYQVRGVLFFRRQEVRRA
                     LSVLSSRTARAAGGGLVDAIEAIWFERLAFRRDQEPDSEEARQRHASLVSLLGVAERL
                     QADGEHADLAGFLAEVQRRAADEAAGVGGGVNLLTYHKAKGLEFDAVLLPALEEGLLP
                     IRQASEPDEVAEERRLLYVGLTRARVHLWVSWSARRTGPSGREQPRKPSRFLDDLVPV
                     DAGRVRPRAVAAGIGTSRAAPGARAGNGRAGEGTAGNGRVEAALSSALGERLRAWRRD
                     RAKQDEVPSYVVFNDRTLAALTERRPRSRGELLAVEGIGPTKLDRYGEELLRILSADE
                     "
     gene            complement(3947..4588)
                     /locus_tag="VF995_01705"
     CDS             complement(3947..4588)
                     /locus_tag="VF995_01705"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9342317.1"
                     /translation="MPMTTIHLVRHGQVENPARVLYGRLPGFNLSARGRAQAELLARY
                     FAAIPVAAVLASPLERAQQTAAPIAAAHGLEVRADPRLIESSSIFEGVAGSVGWYILR
                     HPASWWKLRDIRTPSWGERNVDLAERVQAAVTSAREEFRGQQVVLVSHQAPIWVARLA
                     YQHRPLGHWPGGRRCTLGSVTTIEFDDDHVRRISYAEPAAAPLQGAAPGPAGA"
     gene            complement(4606..5829)
                     /locus_tag="VF995_01710"
     CDS             complement(4606..5829)
                     /locus_tag="VF995_01710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9342318.1"
                     /translation="MPDRVVRRARIAVAVTFFVNGALIGTWAARIPAVKGHLGLDAPQ
                     LGLALLGPTLGALAGIPLAGALVARRGSRPVTRAAVFAHCAVLPLAGLAPDLMALWAA
                     LLLFGLANGLLDVSMNTQGIAVERAWQRPIMASFHGLFSLGALAGALGGSAAAALGLT
                     PAAHFTLAAASLAAIAAGANRWLLAADDGSRQALRLARPSRWLVLLGLIGFCCLFGEG
                     AAGDWSAVYLREVRAASPAVAALAFAAFSLAMTSGRLLGDRATARWGPRLALRAGASL
                     AAGGLLVALLIPTRPAGILGFGLLGAGLSITIPIVFSSAGRGAGGSGDQGQPSGRPPV
                     QQPAGPAIAAVTGMSYLGGLAGPPLIGMVAGQLGLTRALGLIVLLAAVAAVLAGVAPA
                     AATMPRHIPPPAPLD"
     gene            complement(6007..6246)
                     /locus_tag="VF995_01715"
     CDS             complement(6007..6246)
                     /locus_tag="VF995_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342319.1"
                     /translation="MGTDFPSDTSGSSTPTLQREVCDKCGSRMTLQGSAHGFERWECP
                     KCHQIVGIDRDPEVGGRFQIDRGQPWNYSPDAFKN"
     gene            6594..8162
                     /gene="hutH"
                     /locus_tag="VF995_01720"
     CDS             6594..8162
                     /gene="hutH"
                     /locus_tag="VF995_01720"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017614429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX9342320.1"
                     /translation="MPAPVPGSQPAPRRVVLSGDPLTIDDVVAVARQGILAELGDTGR
                     KRMAAAREVVERAVVEGRRVYGVSTGFGLLANTPVAPGDLKELQRRIVLSHATGSGDP
                     LPAEVVRALQLLRARTLTQGFSGARPAVVEALLAHLDAGITPVIPEHGSVGASGDLAQ
                     LAHLALPLLGEGEVELDGRRMPALQALRQAGLEPLRLSYKEGLSLVNGTEGMLALACL
                     AIADAEALATAADLVCALSIEGLLGTDRPFQQRLHELRPHPGQAASAANLRVLLAGSP
                     ILASHRHSDHAIQDAYSLRCAPQVHGACRDTIAFARQVAERELGSVTDNPVVFADSGD
                     VVSNGNFHGEPLAFVLDYLAIALTELASISERRVDRLLDPALNHDLPAFLTPDPGLNS
                     GLMLTQYTAAALVSDCKVLAHPASVDSIPTSGNQEDHVSMGWTAGLKLRRVVANVRRV
                     LGVEALCAAQAIDLRAPLQPGVAVAGALARLRRDVPPLDSDRYLAPDMRAAEVLIERG
                     ELVAAAEQAVGPLR"
     gene            8236..9879
                     /gene="hutU"
                     /locus_tag="VF995_01725"
                     /pseudo
     CDS             8236..9879
                     /gene="hutU"
                     /locus_tag="VF995_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007382525.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
     gene            10194..11351
                     /gene="hutI"
                     /locus_tag="VF995_01730"
     CDS             10194..11351
                     /gene="hutI"
                     /locus_tag="VF995_01730"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX9342321.1"
                     /translation="MFAGPEAELPDLPLAADAVRIDAGGRAVVPGFVDAHTHLVFAGD
                     RADEYAGRLRGVSYEETLAAGGGINRTVRETRAASDAQLERSTAERLVQAGRHGTTTL
                     EVKSGYGLNVADERRSLEVIARLRARTPLDLVPTFLGAHLIPPEFASDRAAYLDLLEH
                     EMLPVCAPLAEFVDAFCDRGALTVEESRRVLRAGKRHGLALKVHANELGPTGGAWLAA
                     EQRCVSADHLLFLDSAEAAAMRRAGTVAVLLPGTSFLLKTGRAAPIQTLRDAGTTVAL
                     GTDCNPGTCYSESMQLMIALACVHGGLTPEEALLAATDGAAKALSRAGRAGRLAPGSA
                     ADLVVLAGRSYADLAYHLGVNLAHQVVKGGRVVASDGRPVALLAPEPEPAD"
     gene            11351..12265
                     /gene="ftcD"
                     /locus_tag="VF995_01735"
     CDS             11351..12265
                     /gene="ftcD"
                     /locus_tag="VF995_01735"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX9342322.1"
                     /translation="MGQDLFESVPNISEGRRGAVVDEIAAAFAGAHPDVRLLDRSSDP
                     DHNRSVLTLAGPGAALVEAAVAGAAACARLIDLTQQDGVHPRMGALDVLPFIPLGTMR
                     LVEGVPLALAAGRRLVAEVGIPVYFYGAAARRPERAALPRVRGRGFEALLTSAASDPD
                     RAPDLGGPAPHPTAGATAIGVRSFLIAYNVNLASDDLAAARSIATAVRERDGGLPAVR
                     ALGLALPGRGLTQVSMNLLDYRVTPPAAAFAAVAALAAQRGVEVAESELVGLIPEAAI
                     DGVDPAELRLRDFSRGRLLEWRLRQEIG"
     gene            12370..13527
                     /locus_tag="VF995_01740"
     CDS             12370..13527
                     /locus_tag="VF995_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342323.1"
                     /translation="MKRGEGLSGPGSGDNPEWFKSPPPLPRVPVTRRAPVAPATAPPP
                     ASTAPPAATGPLALPDEPPTEQLPLPESESIAEPGEPGGPVAAAAPAGQPRAYWFGLA
                     VLVVGLVAIMSSLATHVTSIVQTVLLATPLLLLGVGLERVGRGVGRASLRTLGAVVIV
                     VAVASPVALSLSRPGSQVSTTVDEQVPAGASNGALHVTLGGGQLHVGPGAPGLVRGEL
                     LSPGTPSTEVSTSGKTAVAALAGPRQHGLLARNRGSDWDVRLGSGLPWTLKVDAGPLT
                     ADFDLRQLNVQGVDLSSGISRVALRLNQPAARVPVSLQVSTGLLDVYLPRSASCEIRV
                     TAPVGALVQSNFGSQGLVSQGGAWRTPGESATATFVIEVRVGAGRVRIHRV"
     gene            13671..14402
                     /locus_tag="VF995_01745"
     CDS             13671..14402
                     /locus_tag="VF995_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342324.1"
                     /translation="MTDQPLPSLPMTATVEGANPPPGGGASSEGSGGAGQRRGKLAAA
                     FSTMMLLIGGLAFGACIALLGGYATRLASEVGGGGNPAADAVAPAAHGAAHGAASGAA
                     SGAASSALSDQAAMTVPGDTASISRFGRAAADITVLHVGVAMVESEPLGARPEQGFFV
                     VVKVKVVATGISFDVSSYDFDVLEPDGSPAPRATPVTAWKPPLYAARLSLAGSTGGAL
                     VFDAPARHGLVAYAPHGVPLVEWRF"
     gene            14535..14870
                     /locus_tag="VF995_01750"
     CDS             14535..14870
                     /locus_tag="VF995_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342325.1"
                     /translation="MSMFLARFVAVVAGVAMITLITGSERARDTIGRHSRAGWFYVLL
                     GIAALEVALWRGGFHLMVRTLGAGLLFVVLVPAVQVLFGLIDGVWSWSRRRVDRRTLY
                     RESDSVPWR"
     gene            15014..15574
                     /locus_tag="VF995_01755"
     CDS             15014..15574
                     /locus_tag="VF995_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342326.1"
                     /translation="MVLVWLIVAVTASILLVFVAGAPSGSQTIELIAVAAVVIGGLRY
                     LSLRRLRLQAFMHVRETIKADNLEAYVHQRQALRGAIRSARRERRLVARSTRLGTFYP
                     PIASRPADPGNYVKLRLGGIASPWVRVQDVRGMIIRWTPFVRSPNEAFLTELTEGVAH
                     FWGVDPTVPIRSNLNLTAKSVQFRLP"
     gene            15936..16466
                     /locus_tag="VF995_01760"
     CDS             15936..16466
                     /locus_tag="VF995_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX9342327.1"
                     /translation="MAEPTTSQPPVRVIRSARRRKTVTAYREGDTVVVLLPARLSRRE
                     EAQWVATMLQRLEQRERRRQPATDETLQRRALLLARRYFDGAVEPASVRWVSNMRTRY
                     GSCTPGDGTIRLSDRIAPYPTWVRDYVLVHELAHLAVPDHSAAFWALVARYPMAERAR
                     GFLIAKGLEEHEAADD"
     gene            complement(16463..17767)
                     /locus_tag="VF995_01765"
     CDS             complement(16463..17767)
                     /locus_tag="VF995_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX9342328.1"
                     /translation="MSANPGEGGEFFGRIPLFREFARLLAGESGPVNWELARQVAVAT
                     AAGAEALGATPALPSPAYVPEPAEQRAWDERLRLAELWVEPVGSLPAPPRPLTARLSS
                     RPDWAEAVLPSVAPLVEPAARRVAEATGSAPGLGAEAGVPPEMAGMVGRLGSLIAGLQ
                     VGGVVGQLARLVLGQYDLAIPPADPATVVLLIENVDAFEQASDVPTDQLRLWLACHEV
                     VRQRLFAGVAWLPGHLRELMEEMARLTEPDPAALMEKLQDLNRLDNLQEMLEGGGLLG
                     EPSPALQAATQRLAVLLALTGGYAVGLSGRVLEGRLPALAAIETAVERRFADPAGAHA
                     LFAQLLRVDPSQAAVARGERFCREVLAATDVEGLDRIWAHPNFLPTPEELDAPGRWLE
                     RIGLVGGQEISLEEGLRALLGDDSAGEDEAEPPRAEGPDAGG"
     gene            17950..19020
                     /locus_tag="VF995_01770"
     CDS             17950..19020
                     /locus_tag="VF995_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342329.1"
                     /translation="MARTVALAGAAHPGAGQLALALEDDPAVERVLAFTHAELPMLGP
                     KLEVVPAGPGEERFAKTLAEADTLVVFPLIDATDRSEAGQRDQVLEGVRAALQGATGA
                     ATVVLWSSGIVYGAHPDNPVPLTEAQPIRPNSDFAPAELLAEVERLVLDTAAAGGRGT
                     VVLRGAAIWAPPWGTFLSRLLQAPAIAAVRGYDPPVQGLDPADAASALALATQGGLSG
                     LYNVAPDDWVLASEAARIAGRRRLEIPERVAFTTTERLGRVGVSSAVSGELHYHMHPW
                     VLDNRRLRAAGWAPGSTTAAAMAAAGQALPEGIHVGRIQVRRSDIYRGAAAGLALVAA
                     LAIARQRQARRSPTRRGTSGPS"
     gene            19073..20116
                     /locus_tag="VF995_01775"
     CDS             19073..20116
                     /locus_tag="VF995_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX9342330.1"
                     /translation="MSRALAIDLGTANTLVYVKGQGVRLTQPTVIALNERTGEILAMG
                     DEAYAMIGRTPGHIVAVRPLRGGAISDFDTAARLLRLLLRRVGAGRLTRPKVVICVPS
                     AITPVERRAVQEATLEAGARAAYLLEEPMAAAIGADLPVLEPAGSLIVDVGGGTTEAA
                     CISLGGIVAHNAIRTAGFDLDAAIIQHVRREYAVTIGERSAEAVKIAVGSAIPPHTRG
                     RGGDGADKVTDKVEVRGRELATGLPKTLLLGAAEVHEALEEPVGQIVQAVLDTLSAAP
                     PDLANDVLDKGIFMTGGTSLLRGLRTRIAQETGVPVHLVEQPLDCVVLGAGQVLESFD
                     NLAPLFVGTPGFG"
     gene            20186..21193
                     /locus_tag="VF995_01780"
     CDS             20186..21193
                     /locus_tag="VF995_01780"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX9342331.1"
                     /translation="MRQWVLVGVGAVVLVAVVLTLNFYQLPVVALSPGPAEDVLSRIK
                     VADSTHVYDSKGKLFLTSVGIDDNVRFYQAILDFANPDVQLRPRQELFPKGQSTQQVD
                     QQNASDMDESKQTAMVVALRQLGYHVEPQSVTVAAVLSGAPADGKLQVGDRILRVDDA
                     AVSSIKQVQDTIRRHHVGQTVAFTVSRHDAARTVAVAVGADGGQPRVGVALHEDFGKL
                     PVNLSIDTEDIGGPSAGLMFTLGIIDKLTPGDLTGGRRIAGTGQIEINQTVDPIGGID
                     EKLVAAKREGATVFLVPADNCAEAKAHPPSGLRLVRVRTLTDALDFLRPKAGDPAPQG
                     C"
     gene            21522..24425
                     /locus_tag="VF995_01785"
     CDS             21522..24425
                     /locus_tag="VF995_01785"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX9342332.1"
                     /translation="MVAARRPPRRWTLAVLLLAFVLLAAVGSLSSFYTDLLWFREVGK
                     SPVFWGQLRAKAMLGLLAGAGTALIVGLNLWLVERLAPRYGLAVVGRPQVERAQQILG
                     PYLRPLRLAIAAFIGLVVGLQATGSWQTFLLWRNAVSFTGADATDPQFHRNVGFYVFQ
                     LPFLRAVFGWLFTTLLLAFLLSAAAHYLLGGIRPQAESHRIAAPTQTHLSIMLGLIVA
                     LKAWGYWLNKYELVYSTRGVVATGASYTDVKVQLPALQMLFWVALICALLFFWGARSR
                     GIAVPLFSIVLLAAVSVIVGGVIPTVVQRFRVAPQELQREQPYIQRNIQATRAAFGLS
                     SVDSQPFPDSTNLTAGDVEANASTVDNIRLWDPEVLQPGIQSLQAVAQYYSFSDVDVD
                     RYKVATDPQSQAQLRQVMVAVREVDPTGLTSSAKTWQNLHLAYTHGFGIVATQVNTSA
                     NQGQPDFIVHDFDQTNSPIPIDQPRVYFGEPPPGSTDYVVANSKQPEVDSPNASGGSN
                     TAPYQGDGGIPLSSFPRRLAFALRFRDINLLISGNLTSHSRLMFNRDIRDRVEKVAPF
                     LQWDGDPYPVVVNGKIMWVRDGYTTTANYPYSSRIDLGNAARRASGQRGVQGTGNYIR
                     NSVKAVVDAYTGKVTLYAFDESDPLLRAWRKAFPSLFAPKASMPADLTQHLRYPEDLF
                     SIQTERFASYHIADAAGFYSKGDFWALPQDRSGVIVSQQNAAQQGTAAGLSLATSQAM
                     RPYYLLTKLPGQTDPQFVLVMPFTPNNKENMVSYMAVDSDPQDYGKITLFSLNRTRQV
                     FGPTQVNNRILADSTISSKISLLNQQGSKVILGNLLIVPVKDSLLYVQPIFVQGSGSN
                     SFPLLQNVAVFYNSAVGFDTTLTGAISQALGAQVQTPSTPSAPSTTPPAGLTATGRQL
                     LQQANHEYALAQQSLKAGNLADYQKHVNNMEKLLQQALGSSTSGVTTTTTPKG"
     gene            24498..24574
                     /locus_tag="VF995_01790"
     tRNA            24498..24574
                     /locus_tag="VF995_01790"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24532..24534,aa:Met,seq:cat)
     gene            complement(24658..24834)
                     /locus_tag="VF995_01795"
     CDS             complement(24658..24834)
                     /locus_tag="VF995_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342333.1"
                     /translation="MMTWPDLHVVLALLLALTPAAIIGWAIGAALDRHVHRHVRPFQG
                     PQTTNYRPTTRVGR"
BASE COUNT         3510 a         9033 c         8931 g         3562 t
ORIGIN      
        1 tatggatggc cttcagcatc cgctgatagg cgggcctggg cttgtcgccg gttgctgaga
       61 cgtcgttgtc ggtgtagacg tgagcaatcc gatcgccgcg ccgatcggca ccgtgctggc
      121 agtcctcgat ctgtcgccgc actcccaagg tatcctcggg gtcgagaacc tggctgatgc
      181 gggcgtagat ggcgccggac ttcatgggca taccaccacc ttactatgtg atttgcgact
      241 cgtggcccag cacggcctgc acgtcaacga ggaccacttc ctggtcgagg taatcgaccc
      301 tacgactggg tcgcgggttg cggcggggga gcggggggag ttggtgttca ccacgctgac
      361 caaggaggcg atgccgctgc tgcgctaccg cacgggtgac atcgcgaccc tggaccgctt
      421 gccgtgcccg tgcgggcgca cgctcgtccg gatgtcgaag gtgctcggca gggtcgatga
      481 catgctggtg gtgcgggggg tcaacgtcta tccgagcgag gttgaggcgg tgctgctcgc
      541 cgagcctggg gtgggggcgc agtacctgct ggtggtggat cggacgcgga cgctggcggc
      601 gcttgaggtc gtctgcgagc tggcggaggc ggcgtccggg ggcggggatc aggcgcatca
      661 ggcggagggg gtccgcgccg cggtcgcgct ggcgttggag cgccggctgg ggttgcgccc
      721 cgaggtgcgg gtgctcgccg cggggacgct tccccgtacc gaggttggga aggcgaggcg
      781 ggttgtggag cgttcagcgg agcacgaccc gctgacgctg tagcggtcgc tggagctggt
      841 gcgaagcggc acggcgactg gtttggcgct gcgccgatga gttcgtgcgg gcgaggcggt
      901 cttccttgca ggatcctggc gaagcaggca gcgaagcagg cgtggacagg agggtgatca
      961 tgggcaaggt tgtggtgacg gagttcatct cgctggatgg cgtgatggag gacccgggcg
     1021 gggcggagaa ggaccgctac gagcgtggcg gttggacgtt ccagttcgac cgtggcgagg
     1081 agggcgacaa gttcaagttc gacgagttga tggcgtctga cgcggggctg cttgggcggg
     1141 tgacctacga ggggttcgcg aaggcgtggc cggcgatgga caccgacgac ttcgggcgca
     1201 agctgaactc catgccgaag ttcgtggtct cgtcgacgct ggagaaccca acgtggaaga
     1261 acacgacggt gctccggggc gacctcgctg aggaggtcgg caagctgaag gagcagttcg
     1321 ccggcgacat catggtcgcg gggagcgggc ggctggcgca gtcgctgctg gcgcgcgacc
     1381 tggtggacag gctgcacctg atggtcttcc cggtgctgtt gggggagggc aagcgcctgt
     1441 tcgcggacgg cacggacctg accaggtggc agctcgttga gacgaagcag accggcgagg
     1501 tcgtgatcct cgtcctcgaa cgggagcggt aggccggggc acgacactgg ggcgggtcgc
     1561 ttcgggcgga gggctgtgga taacccggga ggacggtcgg gctgctgtgg cagactccgg
     1621 gcatgaaccc gaagcgactc acccacgatc tgaatcccga gcagcgcgcc gcggtggagg
     1681 cgtgcagcgg cccggtctgc atcctcgctg gtgcggggac tggaaagaca cgggtgatct
     1741 cgcggcgggt cgcctatgcg atcgccaccg gggcggtcga ccccaggcac gtgctggtgg
     1801 tcaccttcac cgagaaggcg gcgggcgaga tgcgcgagcg gctcgccggg cttgggttcc
     1861 cgggggtgca ggcgcagacc ttccatgcgg ccgcgttccg gcagttgcgc tacttctggc
     1921 cacggctctc gcaggagccg ctgccggagg tggccgactc gaaggccgtg ctgctggtgc
     1981 cgctgcagcg cagcctcccg ggcggctaca agttcaccgc cctgaaggac ctcgccgacg
     2041 agatcgaatg ggccaaggcg cggcgcctgg acccggacgg ctacgcggcg gcggtggtgc
     2101 ggctcggccg cacgccgccg gtgccggccg acctgttcac cggcctgttc cgccattatg
     2161 agtgggccaa ggagcgcgcc ggccggatcg atttcgagga catgctggtt cgcatgctcg
     2221 acgcgctgga acgccgcgag gacatcgccg aggagttccg cggccagtat cgctggttct
     2281 cggtggatga gtatcaggac accaacccgt tgcagcaggc gctgctggac gcctggctgg
     2341 gtggccgcga cgacctggcg gtggtcggcg acgaggacca gacgatctat accttcaccg
     2401 gagcgaccag cgactacctg accggtttca agcgccgcta cccggaggcg tgcgtgttcc
     2461 ggctggagca gaactatcgc tccacgccgg aggtgctggc ggtggcgaac cggctgctga
     2521 cccggtcggg cggttcgccg ggcaagcggc tggtggcgac gcgcgcgtcc ggccccgagc
     2581 cggtgatcgc cgccttcgag tcagccgagg gcgaggccgc cgccctggtc gccgaagtcg
     2641 gccgcctggg cgcgctcggg gtggcgcttg aggaggtggc ggtcctgctg cgcaccaacg
     2701 cgcagatccc gctggtggag gaggcgctgg cagccgcggg gatccgctac caggtccgcg
     2761 gcgtgctgtt cttccgccgg caggaggtgc gccgggcgct ttcggtgctg tcgtcccgca
     2821 ccgcgcgggc cgcgggcggg gggctggtcg acgcaatcga ggcgatctgg ttcgagcggc
     2881 tggcgttccg ccgcgaccag gaacctgaca gcgaggaggc acgccagcgc cacgccagcc
     2941 tggtcagcct gctcggcgtc gccgagcggc tccaggccga cggcgagcac gccgacctgg
     3001 ctggcttcct cgccgaggtg cagcgccgcg ccgccgacga ggccgccggg gtgggcggcg
     3061 gggtgaacct gctcacctac cacaaggcca agggcctgga gttcgacgcg gtgctgcttc
     3121 cggctctgga ggaggggctg ttgccgatcc ggcaggccag cgagccggat gaggtggcgg
     3181 aggagcgccg cctgctctac gtcggcctca cccgggcgcg ggtgcacttg tgggtcagct
     3241 ggtcggctcg gcggacgggg ccgtccgggc gggagcagcc ccgcaagccg tcgcgtttcc
     3301 tcgacgacct ggtcccggtg gacgccggcc gggtgcggcc tcgggcggtc gcggcgggga
     3361 tcgggacgtc acgggccgcc ccgggggcgc gggccggcaa cggccgggct ggggagggca
     3421 ccgccggtaa cgggcgggtg gaggccgcgc tcagctcagc tcttggcgag cggctgcggg
     3481 cttggcgtcg ggaccgcgcc aagcaggacg aggtgccctc ctacgtcgtg ttcaacgacc
     3541 ggaccctggc ggcgctgacc gagcgtcgtc cccgtagccg cggggagttg ctcgcggtcg
     3601 aggggatcgg cccgaccaag ctggatcgct acggagagga gttactacga atccttagtg
     3661 ctgacgagta gcttggccgc ttcaggcagg cgagaatagg ctcatgtcac gtcaaaggga
     3721 acggggcgcc catccgcccg tctgtcagta ctgcgcccgg gtcgccgaca ccaacggtct
     3781 cgggttgccg ttcggctgga ctgtccagac gggagcgcac ggcttgacct tcttgtgctc
     3841 aggctgcacc cgctcgaacc tgcgctcgat cgagtcgcgg atgttcgagc gggacccggg
     3901 cgggttcggg ctgaactgag cggctgctcg ggcgctggcg cctgcttcag gcgccagctg
     3961 ggcccggtgc ggcgccttgc agcggcgcgg cggcgggctc ggcgtagctg atgcgcctga
     4021 cgtgatcgtc gtcgaattcg atggtggtca cgctgccgag ggtgcagcgg cgcccgcctg
     4081 gccagtgccc gaggggtcgg tgctggtagg cgagccgcgc gacccagatc ggcgcctgat
     4141 gcgagaccag cacgacctgc tggccacgga actcctccct ggcggaggtg accgccgcct
     4201 gcacccgctc ggcgaggtcg acgttgcgct cgccccatga cggcgtgcgg atgtcgcgca
     4261 gcttccacca gctggccggg tggcgcagga tgtaccagcc gacgctgccg gccacgccct
     4321 cgaagatcga actggactcg atcaggcgcg ggtcggcgcg gacttccaga ccgtgggccg
     4381 ccgcgattgg cgcggcggtc tgctgcgccc gttccagcgg gctcgccagc accgccgcca
     4441 ccgggatcgc agcgaagtag cgggcgagca gctccgcctg ggcacggccg cgagcggaga
     4501 ggttgaagcc gggcaggcgt ccgtacagga cccgagccgg gttctcgacc tgcccatgcc
     4561 gcaccaggtg gatggtcgtc atcggcatgg gtatgacctc gcggttcagt ccaggggcgc
     4621 gggcgggggg atatgtcggg gcatggtagc agcggccggg gcgacaccgg cgagcacggc
     4681 agcgaccgcg gcgagcagca cgatcagccc gagggcgcgg gtaagaccga gctggccggc
     4741 gaccatgccg atcagcggcg gtccggcgag cccgccaaga tagctcatgc ccgtcaccgc
     4801 cgcgatcgcc gggccggcgg gttgttgcac cgggggccta ccgctcggct gaccttgatc
     4861 cccggacccc ccggcgccac ggccggcgct cgagaacacg atcgggatcg tgatcgagag
     4921 ccccgcgccg agcaggccga agccgaggat gccggccggt cgtgtgggga tcagcagggc
     4981 gaccagcagg cccccagcgg ccaggctggc cccggcgcgc agggccaggc gcggtcccca
     5041 gcgggcggtg gcgcggtcac cgaggaggcg gccgctggtc atcgccaggg agaacgcggc
     5101 aaaggcgagg gcggcgacgg ccggggaggc tgcgcggacc tcgcgcaggt acaccgcgct
     5161 ccagtcgccg gcggcgccct cgccaaacag gcagcagaag ccgatcaggc caagcaggac
     5221 gagccagcgg gacggccggg ccagccgcag ggcctggcgt gacccgtcgt cggcggcgag
     5281 cagccagcgg ttggcgcccg ccgcgatggc cgcgaggctg gcggccgcga gcgtgaagtg
     5341 cgccgccggg gtcaggccca gcgcagccgc ggcgctgccg ccgagcgcgc cggccagcgc
     5401 gccgaggctg aacaggccgt ggaacgacgc catgatcggc cgctgccagg cccgctcgac
     5461 ggcgatgccc tgggtgttca tggagacgtc cagcagtccg ttggcgagtc cgaacagcag
     5521 cagcgccgcc cacagggcca tcaggtccgg ggccaagccc gcaagcggca ggacggcgca
     5581 gtgggcgaac accgccgccc gcgtcaccgg tctgctgcca cggcgggcca ccagcgcgcc
     5641 ggccagcggg atgccggcca gcgcgccgag cgtcggcccg agcagggcga gcccaagttg
     5701 gggggcgtca aggccgaggt gccccttgac cgccgggatg cgcgccgccc aggtgccgat
     5761 cagggccccg ttgacgaaga acgtcaccgc caccgcgatc cgcgcccgcc gcacgacccg
     5821 gtccggcatg aacctcgccg tgttcgtcgt ggcgctcacg ctgttcgagt tgggtcgcgg
     5881 tgacacgatc ggcggctgga gagacaaaaa agcccccgga cgggggcttc aacgccacac
     5941 tgaaagttgt cgagctgacc tccagccggt tctgcgggat tatcccgcga tggctggcgg
     6001 cgcgcgctag ttcttgaacg cgtcaggcga gtagttccac ggctggccgc ggtcgatctg
     6061 gaagcggccg ccgacctcgg ggtcgcggtc gatgccgacg atctggtggc acttggggca
     6121 ttcccagcgc tcaaagccgt gcgcggagcc ttgcagcgtc atccgcgagc cgcacttgtc
     6181 gcacacctcg cgctggaggg tcggggtgct gctaccgctg gtatcgctgg ggaagtcagt
     6241 acccatgtcg tcgtctcctt gctcctcccg gtcgttcccc gagatgcacc tccagcatgg
     6301 cccaagtcgc gcgatcttgc atctgccgat cgcgggattc cgcatgccga tctcgtatca
     6361 ggacctcgcc cgatttgtag ctccttgccc tctaggtagt tctaccgttc ctcggtagtt
     6421 ccaaccggct ccgcaccctc tcgcattccc cgcatagctt ctcacgaagc ttcgacgttg
     6481 aggcaatgga cggccacgac attcaattcg gcggaaggga cgccggagtt caccagaggg
     6541 ttcaccagcc tggccccggc gtcggcccca gcagggccgg tacgatgcgg agcatgccag
     6601 caccagtacc gggcagtcag ccagcgcccc ggcgggtcgt gctctccggc gatccgctca
     6661 ccatcgacga cgtggtggcg gtcgcccggc agggcatcct cgcagagctg ggtgataccg
     6721 gacgcaagcg tatggccgcc gcccgcgagg tggtcgagcg ggccgtggtc gaaggccgcc
     6781 gcgtctacgg cgtttcgacc gggttcggtc tgctcgccaa cacccccgtc gccccgggcg
     6841 atctcaagga gctgcagcgc cgcatcgtcc tgtcgcacgc caccggcagc ggcgacccgc
     6901 tgcccgcgga ggtggtccgc gccctgcagc tgctgcgcgc ccgcactctc acccagggct
     6961 tctccggggc ccgcccggcg gtggtggagg cgttgctcgc ccatctggac gccgggatca
     7021 ccccggtcat ccccgagcac ggctcggtcg gcgcctccgg cgacctcgcc cagcttgccc
     7081 acctcgccct gccgctgctc ggcgagggcg aggtcgagct ggatggccgg cgcatgcccg
     7141 ccctccaggc gctccgccag gccgggctgg agccgctgcg gctctcctac aaggagggcc
     7201 tgtcgctggt caacggcacc gaggggatgc tggcgctcgc ctgcctggcg atcgccgacg
     7261 ccgaggcgct ggctaccgcc gccgacctgg tctgcgcgct gtcgatcgag gggctgctcg
     7321 gcaccgaccg gcccttccag cagcgcctgc acgagctccg gccccacccg ggccaggccg
     7381 ccagcgcggc gaacctccgg gtgctgctgg cgggctcgcc gatcctcgcc agccaccgcc
     7441 actccgacca tgccatccag gatgcctact cgctgcgctg tgccccgcag gtccacggcg
     7501 cctgccgcga cacgatcgcc ttcgcccgcc aggtcgccga gcgggagctc ggctcggtca
     7561 ccgacaaccc ggtcgtcttc gccgactcgg gcgacgtggt cagcaacggc aacttccacg
     7621 gcgagccgct ggccttcgtg ctcgactacc tcgccatcgc cctcaccgaa ctggccagca
     7681 tcagcgagcg ccgcgtcgac cggctgctcg accccgccct caaccacgac ctgcccgcct
     7741 tcctgacccc ggacccgggg ctcaactcgg gtctgatgct cacccagtac accgccgccg
     7801 cgctggtgag cgactgcaag gtgctcgccc acccggcctc ggtcgactcg atcccgacct
     7861 ccggcaacca ggaagaccac gtctcgatgg gctggacggc cgggctgaag ctccgccgcg
     7921 tggtcgcgaa tgtccgccgc gtcctcgggg tcgaggcgct ctgcgccgcc caggccatcg
     7981 acctgcgcgc gccgctccag ccaggggtcg ccgtggccgg cgcgcttgcc cggttgcgcc
     8041 gcgacgtgcc gccgctggac tccgaccgct accttgctcc ggacatgcgg gcagccgagg
     8101 tgctgatcga acgcggcgag ctggtggccg cggccgaaca ggcagtcgga ccgctgcggt
     8161 agggagccgc cgcaacgcca caacgacggc aagtgcgcag gaccgagtgc gctggcaagg
     8221 caaggaggaa ccatcatgga aggcgcacgg acggtgcgtg ccccccgggg cccggagcgc
     8281 tcctgtctgg gctggcagca ggaggcggcc ctgcggatgc tctgcaacaa cctcgacccc
     8341 gaggtcgccg agcggcccga cgacctggtc gtctacggcg gcaccggccg ggcggcgcgc
     8401 gactggaagg cgttcgaccg gatcgttgcg acgctgcggc gcctgcgcgg cgacgagacc
     8461 ctgctggtcc agtcgggcaa gcccgtgggg gtcttccgca cccactcgtt cgcgccccgg
     8521 gtgctgctcg ccaactccct gctggtcccc gcctgggcca cctgggagga gttccgcgag
     8581 ctggaggcgg cggggctgac catgtacggg cagatgaccg ccggctcctg gatctacatc
     8641 ggcacccagg ggatcctcca gggcacctac gagaccttcg cggcgctggc agccgcccgg
     8701 ggctggccga gcctggccgg caaggtcgtg ctcacggcag ggctcggcgg catgggcggc
     8761 gcccagccgc tggcggtcac catgaacgag ggcgtggcgc tgtgcgtcga agtcgatccc
     8821 gagcggatcg agcggcgcct ggccaccggc tatctggacg agcgcgccga cgacctcaag
     8881 gacgccgtgg cacgggtgga ggccgcggcg cgggacggcc gggcgctctc ggtcggcctg
     8941 ctcggcaacg ccgccgacgt gctgcccaag ctgctcgccg ccgggttcca gcccgacgtc
     9001 gtcaccgacc agacctccgc ccacgacccc ctcggcggct atgtcccgag cggcatcagc
     9061 cccggcgacg cgcccgccgc ccaggcggcg gacccgagcg gctacgtcaa gcaggcccgc
     9121 gagtcgatgg cggcccactg cgaggcgatg gtcggcttcc tgcgtggcgg cgccgaggtc
     9181 ttcgactacg gcaacaacct gcgtggcgag gcccgggcgg ccgggttcgc cgacgccttc
     9241 gcctaccccg gcttcgtccc cgcctacatc cggccgctgt tctgcgaggg caagggcccc
     9301 ttccgctggg ccgccctgtc gggcgaggag gccgacatcg cccgcaccga ccgggcggtg
     9361 ctggagctgt tcggcgacga ccagcacctg gtccgctggc tgcgcatggc ctcagagcgg
     9421 gtccatttcc agggcctgcc cgcgcgcatc tgctggctcg gctacggcga gcgccaacag
     9481 gccgggctcg ccttcaacca gctggtcgcc cgcggcgagc tggccgcccc gatcgtgatc
     9541 ggccgcgacc acctggactc cggctcggtc gcctccccct accgcgaaac cgaggcgatg
     9601 gccgacgggt ccgacgcgat cgccgactgg ccgatcctca acgcgctgct caacaccgcc
     9661 tccggggcgt cgtgggtgag cgtccaccac ggcggcgggg tcggcatcgg ccgatcgatc
     9721 cacgcgggcg tggtggtggt cgccgacggc accgaggagg ccgcccgccg ggtcgagacc
     9781 gtcctcacca acgaccccgg caccggggtg atgcgccacg ccgacgccgg ctacgaccgc
     9841 gccgtccagg tggcggggga gcgcggcctc gacctgccgt cggtgacgcc ggccaagcgc
     9901 acgggcgagc caggcgacgc tctccatggg aggacgccgg ctgagcgcgc aggcgagcga
     9961 agcgatgccg tccatgggag gccggcggag gtggaggcgg aggcggcgcc gcccgccctc
    10021 cgcacccgga tccggctgga gggcgggcgg tggcggcggt ggccagaggg gcggtcgact
    10081 tcctgctcac cggggccggg cagctggtca ccgcgagcgg tgacgccggc ccggccgccg
    10141 cccggctcgg tgtggtggag cgaggcgcgg tcgcggccag cggcggcacg gtggtgttcg
    10201 ccgggcccga ggcggagctg ccggacctgc cgctggcggc tgacgccgtc cggatcgacg
    10261 ccggtggtcg ggcggtcgtg cccgggttcg tcgatgccca cacccacctg gtgttcgccg
    10321 gcgaccgcgc cgacgagtac gccggccggc tgcgcggcgt ctcctacgag gagaccctgg
    10381 cagccggtgg cggcatcaac cgcaccgtcc gcgagacccg cgccgccagc gacgcccagc
    10441 tggagcgcag taccgctgag cggctcgtgc aggcggggcg ccacggcacc accacgctgg
    10501 aggtcaagtc gggctacggc ctcaacgtcg ccgacgaacg gcgctcgctg gaggtgatcg
    10561 cgcggctgcg ggcgcgcacc ccgctcgacc tggtgccgac cttcctcggc gcgcacctga
    10621 tcccgccgga gttcgccagc gaccgcgccg cctacctcga cctgctggag cacgagatgc
    10681 tcccggtctg cgcgccgctg gccgagttcg tcgacgcctt ctgcgaccgg ggcgcgctca
    10741 cggtggagga gtcccgccgg gtgctgcggg ccggcaagcg ccacggcctg gcgctgaagg
    10801 tccacgccaa cgagctgggc cccaccggcg gcgcctggct ggccgccgag cagcgctgcg
    10861 tctccgccga ccacctgctg ttcctcgact cggccgaggc agccgcgatg cggcgggccg
    10921 ggacggtcgc ggtgctgctg ccgggcacca gcttcctgct gaagaccggt cgggccgcgc
    10981 cgatccagac cctgcgcgac gccgggacca ccgtcgcgct cggcaccgac tgcaacccgg
    11041 gcacctgcta cagcgagtcg atgcagctga tgatcgccct ggcctgcgtc cacggcggcc
    11101 tcacccccga ggaggcgctg ctcgccgcca ccgacggcgc cgccaaagcg ctcagccgag
    11161 ccggccgcgc cggccggctc gcccccgggt ccgccgccga cctggtcgtc ctggccggcc
    11221 gctcctacgc cgacctggcc taccacctgg gcgtcaacct ggcgcaccag gtggtcaagg
    11281 gcggcagggt ggtcgccagc gacggccgcc cggtcgccct ccttgcccca gagccggaac
    11341 cggcggactg atggggcagg atctgttcga gagcgtcccc aatatttcgg agggccggcg
    11401 gggcgcggtg gtcgacgaga tcgcggccgc cttcgccggg gcgcatccgg acgtccgtct
    11461 gctcgaccgc tcctccgacc cggatcacaa ccgctcggtg ctgacgctgg cagggcctgg
    11521 cgcggccctg gtggaggccg cggtggccgg ggcggccgcg tgcgcccgcc tgatcgacct
    11581 cacccagcag gacggcgtcc atccccgtat gggcgccctc gacgtgctgc cgttcatccc
    11641 gctgggaacc atgcgcttgg tggagggcgt cccgctggcg ctggcggcgg gccgccggct
    11701 ggtcgccgag gtcggcatcc ccgtctactt ctacggcgcc gccgcgcgac gaccggagcg
    11761 ggcggcgttg ccgcgagtcc gcggccgcgg cttcgaggcg ctgctgacga gcgctgccag
    11821 cgacccggac cgcgcgcccg acctcggcgg cccggcgccg cacccgacgg ccggggcgac
    11881 cgcgatcggg gtgcgctcct tcctgatcgc ctacaacgtg aacctggcga gcgacgacct
    11941 cgcggcggca cggtccatcg cgaccgcggt ccgtgagcgg gacggcggcc tgccagcggt
    12001 gcgggcactc ggcctcgccc tgcccggccg gggcctgacg caggtctcga tgaacctgct
    12061 cgactaccgg gtgaccccgc cggcggccgc cttcgccgcc gtcgccgcgc tcgccgcgca
    12121 gcgcggcgtc gaggtggcag agtccgagct cgtcggactg atccccgagg ccgccatcga
    12181 cggcgtcgac ccggccgagc tccggctccg cgacttcagc cgggggcgcc tgctggagtg
    12241 gcgcctccgg caggaaatcg ggtaggaaca ccccgcgcga ttggcgctct ccagccaggc
    12301 gtgctcgaat gcgactggcg atatcgagca gcgacagcgc agcgacccgg ccgtggcggg
    12361 aggagaacgg tgaagcgcgg agagggcctg tcgggcccgg gcagtggcga caaccccgag
    12421 tggttcaaga gcccaccacc gctgcctcgg gtgccagtca cccgcagggc accggtcgcg
    12481 cccgcgacgg cgccgccgcc tgcctcgacg gcgccgccgg cagcgaccgg cccgctggcc
    12541 ctccctgacg agccgccgac cgagcagctt ccgctgcccg agtccgagtc cattgccgag
    12601 cccggcgagc cgggcggccc cgttgcggcc gccgcgccgg cgggccagcc gcgcgcgtac
    12661 tggttcggcc tggcggtgct ggttgtggga ctggtggcga tcatgtcgtc gctggcgacc
    12721 cacgtgacct cgatcgtcca gacagtcctg ctggccaccc cgctgctcct gctcggtgtg
    12781 gggctggagc gggtcggccg cggggtcggt cgcgcctcgc tgcgcacgct cggcgccgtg
    12841 gtgatcgtgg tggccgtggc gagcccggtg gcgctgtcgc tctcccggcc cggctcgcag
    12901 gtcagcacca ccgtggacga gcaggtcccg gcgggcgcca gcaacggggc cctgcacgtc
    12961 accctcggcg gcggccagct ccacgtcggc ccgggggctc ccgggctggt ccggggcgag
    13021 ctgctcagcc cggggacgcc gagcaccgag gtctccacca gcggcaagac cgccgtggca
    13081 gccctggccg gaccgcgcca gcacgggctg ctcgcccgca accgcggcag cgactgggac
    13141 gtccggctgg gcagcggcct gccatggacg ctcaaggtcg acgccgggcc gctgaccgcc
    13201 gacttcgacc tccgccagct caacgtccag ggcgtcgacc tctcctccgg catctcccgg
    13261 gtcgccctgc gcctcaacca gccggccgcc agggtccctg tcagcctgca ggtctcgacc
    13321 gggctgctcg acgtctacct gcccaggagc gcctcctgtg agatccgggt gacggccccc
    13381 gtgggcgctc tggtccagag caacttcggc tcccagggcc tggtgtccca gggaggagcg
    13441 tggcgtacac cgggggagag cgcgaccgcc accttcgtga tcgaggtccg cgtcggcgcc
    13501 ggccgagtcc gcatccaccg cgtctgaccc ccgagcggcc cagcgtccgg cggtgtcagg
    13561 ggacagctgc ttgctggtga ggcaaactac gtagaaaacg cgactggctc gctccctgtg
    13621 aaacgtagaa aatgccccac cgacttgaag ttgccaacag agcggacgtc atgaccgacc
    13681 aacctcttcc cagcctcccg atgaccgcga ccgtggaggg cgccaatccg ccgccgggcg
    13741 gcggtgcttc atccgagggc agcggcggtg ccgggcagcg ccgcggcaag ctcgccgcgg
    13801 cgttctccac catgatgctg ctgatcggcg gccttgcctt cggcgcctgc atcgccctgc
    13861 tcggcgggta cgccacccgg ctcgccagcg aggtcggcgg tggtggcaac ccggccgccg
    13921 acgccgtcgc gccagctgcc catggggcgg cccatggggc ggccagtggg gcggccagtg
    13981 gggcggccag cagcgccctg agcgaccagg ctgcgatgac cgtgccgggc gacaccgcct
    14041 cgatctcgcg gtttgggcgg gccgccgccg acatcaccgt gctccacgtg ggcgtggcca
    14101 tggtcgagtc ggagcccctc ggggcgcggc cggagcaggg cttcttcgtg gtggtgaagg
    14161 tcaaggtggt ggcgacgggc atcagcttcg acgtcagctc ctacgacttc gacgtcctgg
    14221 aacccgacgg gtcgccggcg ccacgggcga ccccggtgac cgcttggaag ccgccgctct
    14281 acgccgccag gctcagcctg gcgggcagca ccggcggggc cctggtgttc gacgcgcccg
    14341 cgcggcatgg gctggtcgcc tacgcccccc atggcgtgcc gctggtggag tggcggttct
    14401 gagccggttc cgcggggacc cgtcgttgat ggccggcgtt catggcatcg acgttgccga
    14461 tgccgcctcg acagggatgg cgcccccggt tatagtgagt aagtcgaaca tgtgttcgac
    14521 agggaggtgc ccgcatgtcc atgttcctgg cccggttcgt cgcagtcgtc gccggtgtcg
    14581 ccatgatcac cctcatcacc ggcagcgagc gcgcccgcga cacgatcggc cgccactccc
    14641 gcgccggctg gttctacgtc ctgctcggca tcgccgcgct tgaggtggcg ctgtggcgcg
    14701 gcggtttcca tctcatggtg cgcaccctcg gcgccggact gctgttcgtc gtgctggtac
    14761 ccgccgtcca ggtgctgttc ggcctgatcg acggcgtctg gtcctggtct cggcgccgtg
    14821 tcgatcgccg caccctctac cgcgagagcg acagcgttcc ctggcgctga cccgcgcagc
    14881 caagcgtcag ccctgagcag gttccaccct ggcagattct gctcaggtat tgcggttccc
    14941 cggcggctat gtgctaattg cgatactcgc gcgtggagcg ctgcgttcgg ggctaaggag
    15001 ggtgaaccgc tccatggtac tggtgtggct gattgtcgct gtgaccgcgt cgatcctgct
    15061 cgtgttcgtc gccggggcgc ccagcgggtc ccagaccatc gagttgatcg cggtcgccgc
    15121 cgtggtgatc ggcggcctgc ggtacctgag ccttcgccgc ctgcggctgc aggcgttcat
    15181 gcatgtcaga gagacgatca aagcagacaa cctggaagcg tacgtccacc aacgccaggc
    15241 gctccgcggc gccatccgca gcgcgcggcg cgagcgtcgg ctggtggctc gatccaccag
    15301 gctcggcacc ttctatccgc cgatcgccag ccgacccgcc gacccgggca actacgtcaa
    15361 gctccgcctt ggcgggatcg cctccccctg ggttcgcgtc caagacgtcc gcggcatgat
    15421 catccgctgg actccgttcg tgcgctcccc gaacgaggcc ttcctcaccg agctgaccga
    15481 gggtgtcgcc cacttctggg gcgtcgaccc gaccgtgccg atccgctcca acctgaacct
    15541 gacggccaaa tcagtccagt tcaggcttcc gtagcggaaa ccgcgaccaa acgcccgcaa
    15601 gaacgcgctg acgaggcccc ggtgaccgtg ggtcccgctc cctgtcttcc ccttacaggg
    15661 agatggccgt tgtctcaccg gaggcctcgt cgtcagcacg atcgcatgcc gccccgggcg
    15721 cgtcaacgaa cctgtcccca gggtctgtgg atagtgctgg ggacaacctg acctgcgctg
    15781 ttgatcatgg atcgggaact gtggacaacc agcgggcggc tggggacagg gagatggaca
    15841 ttcggtgtcc actaccacga tgacctgccc cgcgctcgcc aaagcctgtg gacagagaaa
    15901 tatttctgtc acaccggcgt gccatactcc cccggatggc cgaaccaacc acctcgcagc
    15961 cgcccgtccg ggtgatccgc agcgcccggc gccgcaagac cgtgaccgcc taccgcgagg
    16021 gcgacacggt cgtcgtcctc ctccccgccc ggctcagccg gcgggaggag gcgcagtggg
    16081 tcgccaccat gctgcagcgc ctggagcagc gtgagcgccg ccgccagcca gcgaccgacg
    16141 agacgctgca gcggcgcgcg ctgctgctcg ccaggcgcta cttcgacggc gcggtcgaac
    16201 cggcctcggt gcgctgggtc agcaacatgc gcacccgcta cggttcctgc accccggggg
    16261 acggcaccat ccgcctctcc gaccgcatcg ccccctaccc gacctgggtg cgcgactacg
    16321 tgcttgtcca cgagctggcg cacctcgccg tcccggacca ctccgccgcg ttctgggcgc
    16381 tggtcgcccg ctacccgatg gccgagcgcg cccgcggctt cctgatcgcc aaaggcctgg
    16441 aggaacacga agccgccgat gactagccgc cggcgtccgg gccctcggct cgcggcggct
    16501 ccgcttcgtc ctcgcccgcg ctgtcgtcgc cgagcagggc gcgcaggccc tcctccaagc
    16561 tgatctcctg gccgccgacc aggccgatcc gctccagcca gcggccgggc gcgtcgagct
    16621 cctcgggggt cggcaggaag ttgggatgcg cccagatccg gtcgagtccc tcgacgtcgg
    16681 tggcagccag gacctcgcgg cagaaccgct cgccccgcgc gaccgcggcc tgcgacggat
    16741 cgacccgcag cagctgcgcg aacagggcat gcgcgccggc ggggtcggcg aagcggcgct
    16801 ccaccgccgt ctcgatcgcc gccagggcgg gcaggcgccc ctccagcacc cggccgctga
    16861 gccccaccgc gtacccgccg gtcagcgcca gcagcacggc gagccgctgg gtggcggcct
    16921 ggagcgccgg gcttggctcg ccgagcagcc caccaccctc gagcatctcc tgcaggttgt
    16981 cgagccggtt caggtcctgg agcttctcca tcagcgcggc cgggtccggc tcagtgagcc
    17041 tcgccatctc ctccatcagc tcgcgcagat ggccgggcag ccacgccacc ccggcgaaca
    17101 gccgctggcg gaccacctcg tggcaggcga gccagagccg cagctggtct gtcgggacgt
    17161 cgctggcctg ctcgaaggcg tccacgttct cgatcagcag caccaccgtg gccggatcgg
    17221 caggcgggat cgccaggtcg tactggccga gcaccaggcg ggcgagctgg ccgaccacgc
    17281 cgccgacctg caggccggcg atcaggctgc cgagccgtcc gaccatcccg gccatctccg
    17341 gcggcacgcc agcctcggcc ccgaggcccg gggccgagcc ggtcgcctcg gccacgcggc
    17401 gggcggccgg ttcgaccagg ggtgcgaccg acggcagcac cgcctcggcc cagtccggcc
    17461 ggctgctcag ccgggcggtg aggggacgcg gcggcgccgg caggctgccc accggttcga
    17521 cccacagctc ggcgaggcgc agacgctcgt cccaggcacg ctgctcggcc ggctcgggaa
    17581 cgtacgcggg gcttggcagc gccggcgtgg ccccaagcgc ctcggccccg gcggccgtcg
    17641 ccaccgccac ctggcgggcc agctcccagt tgacggggcc cgactcaccc gccagcagcc
    17701 gcgcgaactc gcggaacagg gggatcctgc cgaagaactc cccgccctca cctgggttgg
    17761 cactcatgca cccaatccta ctcgcgcaac ccccgcccca agccgcgcca agccggtgga
    17821 ttgtgtcctg gttcaccggt gatgctccgg tcataagggt ggcaggaacg gtagcctgaa
    17881 gccgacggca aacggcgttg gcaagccgat ggcatcgggg ctccccgaga cgtggaaggg
    17941 gcggaagcgg tggcacgaac ggtcgccctg gcaggggcag cacatccggg agcggggcag
    18001 ctcgctctgg cgctggagga cgacccggcg gtcgagcggg tcctcgcctt cacccatgcc
    18061 gagctgccca tgctcggccc gaaactggag gtcgtaccgg cgggccccgg cgaggagcgc
    18121 ttcgccaaga ctctggccga ggccgacacg ctggtggtgt tcccgctgat cgacgcgacc
    18181 gaccgcagcg aggccggcca gcgggaccag gtcctggagg gcgtgcgggc cgccctccaa
    18241 ggggccacag gcgcggccac cgtggtgctg tggtccagcg ggatcgtcta cggcgcccat
    18301 cccgacaacc ccgtccccct caccgaggcg cagccgatcc gcccgaacag cgacttcgcg
    18361 cccgcggagc tgctggccga ggtggagcgc ctggtgctcg acaccgccgc cgccggcggc
    18421 cgcggcacgg tggtgctgcg cggcgccgcg atctgggcgc cgccatgggg gacgttcctg
    18481 tcgcggctgc tgcaggcgcc agcgatcgct gcggtgcgcg gctacgaccc gccggtgcag
    18541 ggcctcgacc ccgccgacgc ggccagcgcc ctggccttgg cgacccaggg cggactgagc
    18601 ggcctctaca acgtcgcccc ggacgactgg gtgctggcca gcgaggccgc caggatcgcc
    18661 gggcgccgcc gcctggagat ccccgaacgg gtcgccttca ccactaccga acggctcggc
    18721 cgcgttggcg tgtccagcgc cgtctccggt gagctgcact accacatgca cccatgggtg
    18781 ctcgacaacc gccgcctgcg cgccgccggc tgggcaccgg gttcgaccac cgccgcggcg
    18841 atggccgcag ccggccaggc cctgcccgag gggatccacg tcggccggat ccaggtgcgg
    18901 cgcagcgaca tctaccgggg cgccgccgcc gggctcgcgc tggtcgccgc actcgccatc
    18961 gcacgccagc ggcaggcgcg ccgctcgccg acgcgccgcg gcaccagcgg tccgtcatga
    19021 gtccgtcatg agcgggtcgc gctgaggcca ccatgctgta agagggattc cgatgagcag
    19081 ggcgctcgcg attgacctcg gcaccgccaa caccctggtc tacgtcaagg gccagggcgt
    19141 ccggctcacc cagccgacgg tcatcgcgct caacgagcgg accggcgaga tcctcgccat
    19201 gggcgacgag gcctacgcca tgatcggccg gacccctggc cacatcgtgg cggtgcggcc
    19261 gctgcggggc ggcgcgatct ccgatttcga caccgccgcg cggctgctcc ggctgctgct
    19321 gcggcgcgtc ggcgccggcc ggctgacccg gccgaaggtg gtgatctgcg tcccgtcggc
    19381 gatcaccccg gtggagcggc gggccgtgca ggaggcgacc cttgaggccg gggccagagc
    19441 cgcctacctg ctggaggagc cgatggcggc ggcgatcggc gccgacctgc cggtgctgga
    19501 gccggcgggc agcctgatcg tggacgtggg cggcggcacc accgaggcgg cctgcatctc
    19561 acttggcggg atcgtcgctc acaacgccat ccgcaccgcg ggcttcgacc tggacgccgc
    19621 gatcatccag catgtgcggc gtgagtacgc ggtcaccatc ggcgagcgga gcgccgaggc
    19681 ggtgaagatc gccgtgggct ccgcgatccc gccccacacc cggggccgcg ggggcgacgg
    19741 cgccgacaag gtcaccgaca aggtcgaggt gcgcggccgc gagctggcca ccgggctgcc
    19801 caagaccctg ctgctcggcg ccgccgaggt ccacgaggcg ctggaggagc cggtggggca
    19861 gatcgtgcag gcggtcctgg acaccctctc ggcggccccg ccggacctcg ccaacgacgt
    19921 cctcgacaag ggcatcttca tgaccggcgg gaccagcctg ctgcgcggcc tgcgcacccg
    19981 catcgcccag gagaccggcg tcccggtcca tctggtggag cagcccctcg actgcgtggt
    20041 gctgggcgcc ggccaggtgc tcgagtcgtt cgacaacctg gcgccgctgt tcgtcgggac
    20101 gcccgggttc ggctgagaca tcagacgcgg ccgatgcacc ttatgatgcg ttgagaggca
    20161 ggagcgggtg ggagcgggag gtcgcgtgcg gcagtgggtc ctggtcggcg tcggtgcggt
    20221 ggtcctggtc gccgtcgtgc tgacactgaa tttctaccag ctcccagtgg tggcgctgtc
    20281 gcccggcccg gccgaggacg tgctcagccg catcaaggtg gctgacagca cccacgtcta
    20341 cgactccaag gggaagctct tcctgacctc ggtcgggatc gacgacaacg tgcgcttcta
    20401 ccaggcgatc cttgatttcg ccaaccctga cgtccagctg cgcccccgcc aggagctgtt
    20461 ccccaagggc cagagcaccc agcaggtcga ccagcagaac gccagcgaca tggatgagtc
    20521 caagcagacc gcgatggtgg tggcgctgcg ccagctcggc taccacgtcg agccgcagag
    20581 cgtgacggtc gccgccgtgc tcagcggcgc gcccgccgac ggcaagctcc aggtcggcga
    20641 ccgcatcctg cgggtggacg acgccgcggt cagcagcatc aagcaggtcc aggacaccat
    20701 caggcgccac cacgtcggcc agacggtcgc cttcacggtc agccgccacg acgccgcccg
    20761 caccgtggcg gtcgcggtcg gggccgacgg gggccagccg cgcgtcggag tagcgctcca
    20821 cgaggacttc ggcaagctgc cggtgaacct ctcgatcgac accgaggaca tcggcggccc
    20881 ctcggccggg ctgatgttca ccctggggat catcgacaag ctcaccccgg gcgacctgac
    20941 cggcggccgc agaatcgccg ggaccggcca gatcgagatc aaccagacag tcgacccgat
    21001 cggcgggatc gacgagaagc tggtcgcggc caagcgggag ggcgccaccg tcttcctggt
    21061 ccccgccgac aactgcgccg aggccaaggc ccacccgcca agcggcctgc gcctggtgag
    21121 ggtgcgcacc ctgacggacg cgctcgactt cctgcggccg aaagctggcg acccagcgcc
    21181 acagggctgc tgacgccagc gggggaggcc gctcaggagc tgcgcgggcc gccgccggtc
    21241 gccgccagga tcacgctgcc agccagcagc gcgaacgagg tcgcgatgat caggtcgatg
    21301 aacgttgccg ataccccctt gacgtcccgc aagcggtggc cctcgccggt acaggcgacg
    21361 gcgcgccgaa aacggtacgg gagtcatacc actttgccat ggggcccctg tcgacgccct
    21421 gtggacagtc gccttcgctc tggccgccca agtgctaggg tctggcctag aaacaccctg
    21481 gtccccgcaa cgaaacgtcg tggagtgaaa ggtctggtca cgtggtcgca gcgcgtcgcc
    21541 ctccccgccg ctggacgctc gccgtcctgt tgctggcgtt cgtcctgctg gccgcggtcg
    21601 gcagcctctc cagcttctat accgacctgc tctggttccg cgaagtcggc aagagccccg
    21661 tgttctgggg ccagctccgc gccaaggcca tgctcggcct gctggcgggc gcgggcaccg
    21721 cgctgatcgt ggggctgaac ttgtggctgg tcgagcggct cgcgccccgc tacggcctcg
    21781 cggtggtcgg ccggccccag gtggagcgcg cccagcagat cctcggcccc tacctgcgcc
    21841 cgctgcggct cgccatcgcc gccttcatcg gcctggtcgt cgggctgcag gcgaccggct
    21901 cgtggcaaac cttcctgctg tggcgcaacg cggtctcctt caccggcgct gacgccaccg
    21961 acccgcagtt ccaccgcaac gtcggcttct acgtcttcca gcttcccttc ctgcgggcgg
    22021 tgttcggctg gctgttcacc accctgctgc tcgccttcct gctgtccgcc gccgcccact
    22081 acctgctcgg cggcatccgc ccgcaggccg agtcccaccg catcgccgcg ccgacccaga
    22141 cccacctgtc gatcatgctc ggcctgatcg tcgcgctgaa ggcctggggg tactggctca
    22201 acaagtacga gctggtctac tcgacccgcg gggtggtggc gacgggggcc tcctacaccg
    22261 acgtgaaggt gcagctgccc gcgctccaga tgctgttctg ggtggcgttg atctgcgcgt
    22321 tgctgttctt ctggggggcg cgcagccgcg gcatcgcggt accgctgttc agtatcgtgc
    22381 tgctggccgc cgtctcggtg atcgtcggcg gagtgatccc cacggtcgtg cagcgcttcc
    22441 gggtcgcccc gcaggagctg cagcgcgagc agccctacat ccagcgcaac atccaggcca
    22501 cccgcgccgc gttcgggctg tcgtcggtgg actcgcagcc gttcccggac agcaccaacc
    22561 tgaccgcggg cgatgttgag gccaacgcca gcaccgtcga caacatccgc ctgtgggacc
    22621 cggaggtgct gcagccgggc atccagagcc tgcaggcggt tgcccagtac tactcgttca
    22681 gcgacgtgga cgtcgaccgc tacaaggtcg cgaccgaccc ccagtcccag gcgcagttgc
    22741 gccaggtgat ggtcgccgtg cgcgaggtcg acccgaccgg gctcacctcc tccgccaaga
    22801 cctggcagaa cctgcacctt gcctataccc acggcttcgg gatcgtggcg acccaggtga
    22861 acacgtcagc gaaccagggc cagccggact tcatcgtcca cgacttcgac cagaccaact
    22921 cgccgatccc gatcgaccag ccccgcgtct acttcggcga gccgccccct ggctcgaccg
    22981 actacgtcgt ggccaactcc aagcagccgg aggtcgacag ccccaacgcc agcggcggca
    23041 gcaacaccgc gccctaccag ggcgacggcg gcatcccgct gtcgagcttc ccccgccggc
    23101 tcgccttcgc gttgcgcttc cgcgacatca acctgctcat ctcggggaac ctgaccagcc
    23161 attcgcggct gatgttcaac cgcgacatcc gcgaccgggt cgagaaggtc gccccgttcc
    23221 tgcagtggga cggcgacccc tacccggtgg tcgtcaacgg caagatcatg tgggtgcgcg
    23281 acggctacac caccaccgcg aactacccgt actcgtcgcg gatcgacctc ggcaacgccg
    23341 cccgccgcgc cagcggccag cgtggcgtcc aaggtaccgg caactacatc cgcaactcgg
    23401 tcaaggccgt ggtcgacgcc tacaccggca aggtgacgct gtacgccttc gacgagagcg
    23461 acccgctgct gcgggcctgg cgcaaggcgt tccccagcct gttcgccccc aaggcgagca
    23521 tgcccgccga cctcacccag cacctgcgct acccagagga cctgttctcc atccagaccg
    23581 agcgcttcgc cagctaccac atcgccgacg cggctggctt ctactccaag ggcgacttct
    23641 gggcgctgcc gcaggaccgc tccggcgtga tcgtctcgca gcagaacgcc gcccagcagg
    23701 ggaccgccgc cgggctgagc ctcgcgacga gccaggcaat gcggccctac tacctgctga
    23761 ccaagctgcc cggccagacc gatccacagt tcgtgctggt gatgccgttc accccgaaca
    23821 acaaggagaa catggtctcc tacatggcgg tggactccga cccgcaggac tacggcaaga
    23881 tcaccctgtt cagcctgaac cgcacccggc aggtgttcgg cccgacccag gtgaacaacc
    23941 ggatcctggc cgactccacg atctcctcca agatctccct gctcaatcag cagggctcca
    24001 aggtgatcct cgggaacctg ctgatcgtcc cggtgaagga ctcgctgctg tacgtccagc
    24061 cgatcttcgt gcagggctcg ggcagcaact ccttcccgct gctgcagaat gtcgcggtct
    24121 tctacaacag cgccgtcggc ttcgacacca ccctgacggg cgcgatctcg caggcgctcg
    24181 gtgcgcaggt ccagacgccc tccacgccgt cggcgccctc gaccaccccg cccgccggcc
    24241 tcaccgcgac cggccgccag ctcctgcagc aggccaacca cgagtacgcg ctcgcccagc
    24301 agtcgctcaa ggcgggcaac ctggccgact accagaagca cgtcaacaac atggagaaac
    24361 tgctgcaaca ggcactcggg tcctccacca gcggcgtcac caccacaacc accccgaagg
    24421 ggtgaccgga cggcccgatc acctcgccaa attgctttgc ggccggttgg cgacgtgtgc
    24481 taccgtgcgg ctctcaccgc ggggtggagc agtctggtag ctcgctgggc tcataaccca
    24541 gaggtcgagg gttcaaatcc ctcccccgcc accaagcagg tcagaagtgc tccgaggttc
    24601 tcggagcgct tcttcttttg tcgtcgcttc gtgccgccag tgcggtcgag gagccagtta
    24661 gcggccaact ctggtggtcg ggcggtagtt ggtcgtctgc gggccctgga aggggcggac
    24721 gtggcggtgg acgtggcggt cgagtgcggc gccgatcgcc cagccgatga tcgcagccgg
    24781 ggtgagggcc aggagtaggg ccagaaccac gtgcaggtca ggccaggtca tcatcaactc
    24841 ctcctttcac tgccgaaggt gagtaccgcg ccaagtgcgc ctcgaatcgc gaagttgccg
    24901 attggtgatc gagacgagtg cggagacagg attgtcaagc tcgatcctgc tgggtattct
    24961 acggcattag cagtagggct cgatacggca cgcggaccgt cgctgtcacc attgctcgtc
    25021 atctcgagtt cgaggg
//
LOCUS       DASWDC010000055         7458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_339541, whole genome shotgun sequence.
ACCESSION   DASWDC010000055 DASWDC010000000
VERSION     DASWDC010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339541"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VF995_01800"
     CDS             <1..594
                     /locus_tag="VF995_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9342334.1"
                     /translation="MRSLVMLGVATGARLGELLALRWDHVDLEAGTLKIGWSRRVVNN
                     RMEVKAPKTESGARTLVLGPATVAALRRLRAEQAGRRLALGGAYHHEDLVICKPDGQP
                     YRPDSVSGMFRMFVDKAGLPKAVHVHTLRHSAASFQAAAGVPASDIAAQLGHKDGGAL
                     ALRVYVHPMAEGLARAGAVLDRVVGAGGGGGNSSTAT"
     gene            887..1456
                     /locus_tag="VF995_01805"
     CDS             887..1456
                     /locus_tag="VF995_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342335.1"
                     /translation="MEGACHGRNHREQHKRWKEAKDQGEGQPYAGTARVGFGGQAGSD
                     AECLGAGRERLGRGHSPPFRRIERGGGSAQLVKSSPLGGGTQGRGGRASKVEVRRHPA
                     KLRAERFGAAFSDPLDSKVRVEPGEEAGAEQIDRCRCRFRQRCALSLFGPSASDAHRE
                     KDRRPAEGNRQADDAPGAERHGGEHQCCR"
     gene            complement(2254..2841)
                     /locus_tag="VF995_01810"
     CDS             complement(2254..2841)
                     /locus_tag="VF995_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9342336.1"
                     /translation="MGAIVTRRLPGQGRRRRERLEAAVPEVLDVLRATVVAGAAPRRA
                     LQAAAEVAPPPLQRLLAEAVRAAEFGAGAGQALAAVGRKEPLAELVLAGEALDLAEAT
                     GAPPARVLAGVAAAASDRLRATQATLAATAQARLSARVVAAMAPAFLALLALISPADV
                     TFLVRGRFGWATLAAATAFEAAGIWWADRIVRGTR"
     gene            complement(2886..4115)
                     /locus_tag="VF995_01815"
     CDS             complement(2886..4115)
                     /locus_tag="VF995_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX9342337.1"
                     /translation="MAERVQHRLSGLSGGLQSASGADLRVVVADTLRDEGIVLPASSF
                     ATTVQAVTDELTGLGPISPLLADPAVTDIMINGPDDVWVERNGRIERTAIHLASSAAL
                     AALIQRVVAPLGLRVDEARPWVDARLPGGERFHAVLPPLAPDGPLVTIRTFARQRLTL
                     ADLVDRGTLDATTARLLEAMVSAGIATVASGATGTGKTTLLNVLAAAIPPSERVVTIE
                     DAAELSLNRPHVVRLEARPSNVEDRGEVTLRELVRNALRMRPDRIVVGEARGPEVMDL
                     LQAANTGHRGLLATLHAAGPAEVPARLEALALSAPGAVLEVVRRLIAGGIEAVVHLER
                     TPTGRRVTAVAALTLDHYGQAQAKLVRVAGPEGLDATGYVPSWADRLDPAVLDAFEPS
                     SPAGRVRVLAARRGGHR"
     gene            complement(4433..5716)
                     /locus_tag="VF995_01820"
     CDS             complement(4433..5716)
                     /locus_tag="VF995_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342338.1"
                     /translation="MALHPARAPLPLVVIAHEDPGTAESLRHAVETAAGWRATVADQS
                     PGGLSAALAASPALALVGCGALAELPAGCRVPLLVIGDDSRPADLRAALAVGARGLLP
                     WPDGAADLPAELARIAAETRPSKAVREHFTAPVIAVLAAHLAGAWSRFGPAPILLADL
                     AGGLGYRLDISPAAPTWSDLAPAPGFQPCDLEVAAAVGLSDLTATSGLHAPQPDNGAT
                     PFHAPAPPTETEPEALLGPCPACPRHALEWDGEGLAAALAEPWPGLSVLPLAGLIDGG
                     PEPPPDPCLIQAIFDLARSVYRVVVVDLPPTNSFMFETALELADVLLAVGRCESAGVQ
                     GLLAALEGWSALDRDPSTAGAVLTGVRSRAPLAPKDARKDLHDRLWAAIPAAPRELST
                     AAEEATLLLDREELPAVQAMLTLANRVVPFPAVAG"
     gene            6179..7402
                     /gene="fabD"
                     /locus_tag="VF995_01825"
     CDS             6179..7402
                     /gene="fabD"
                     /locus_tag="VF995_01825"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX9342339.1"
                     /translation="MTVAFVFPGQGSQAPKMGLDWADDGSGDLYGWASACLGWDLAET
                     LAHATPDELRQTYIAQPAIFCVSVAALQALERAGVRPSFVAGHSLGEFSALVAAGVLS
                     FEAGLALVARRAEAMQRAADARPGSMSSVLGLSVEGVEMAVAAAAGNNVLAIANDNAP
                     GNVVVSGDFPALERLPVAAKELGAKRVVPLNVGGAFHSPLMGPAAEAFWPFLAAAPLR
                     DAAVPVVANATAEPVTVAAELRELLGRQLTGPVRWTESVRRLADLGVDTFVEVGPGTV
                     LSGLIKRTLDGVRVLSAGDAAGVAAVAETLREQPDSPQASAASDGEDQGEVVVLPERF
                     AVSPGHGRFYPAAPSRFTDEGAYVEPGDLLGEVRNGNASIPVRSPFRGWVMAHLAWEG
                     ELVTPGQLLLSLRPF"
BASE COUNT         1013 a         2401 c         2983 g         1061 t
ORIGIN      
        1 atgcgcagcc tggtcatgct cggcgtggcg accggcgccc ggctcgggga gctgctggcg
       61 ctgcgctggg atcacgtcga cctggaggcg ggcacgctca agatcggatg gtcacgccgg
      121 gtcgtcaaca accgcatgga ggtgaaggcg ccgaagactg agagcggcgc ccgcaccctg
      181 gtcctcggcc cggccaccgt cgcggcgctc aggcggctgc gcgccgagca ggccgggcgg
      241 cgcctcgccc tcggtggcgc ctaccaccat gaggatctgg tgatctgcaa gccggacggg
      301 cagccctacc ggccagactc ggtgtccggg atgttccgaa tgttcgtcga caaggccggg
      361 ctcccgaagg cggtgcacgt ccacacgctc aggcacagcg cggcgagctt ccaggcggcg
      421 gccggcgtgc ccgcctccga catcgccgcg cagctcggcc acaaggacgg cggcgcgctc
      481 gccctccgcg tctacgtgca cccgatggct gaaggtctcg cccgtgccgg cgcggtcctc
      541 gaccgggtgg tcggcgcggg agggggaggc ggcaacagca gcacggcgac gtagggacgg
      601 cgggtcgccg ggactgtttg gctgcaagtt ggctgcaaag agcgccgccg tacgggacag
      661 gtacgcggcg ctctttgcgt ttccgcaggt caaagaggtg aatgggtatc ggggcggcgg
      721 ggcgcaaggg cgtactcaac ggattcgctg cctgcttcgg cttccttgac gaattcgcgg
      781 aaggggaatc gggagcggat acggctggca atgaacatca cggactcctt tgtgattctg
      841 tggttgtggt tcatgggtgt gtcatcacgg gctacggcgc taaggcatgg agggcgcctg
      901 ccatggtcgg aatcaccgtg agcagcacaa acgctggaag gaagcaaagg accaggggga
      961 aggccagccg tacgccggca cggcccgcgt cggcttcggc ggccaggcgg gctcggatgc
     1021 ggagtgcctc ggcgcgggcc gcgagcgcct cggacggggg cattccccac cgttccgccg
     1081 catcgagcgc ggcggcggca gcgcgcagct cgtcaagtcc agtccgctcg gaggcggcac
     1141 gcagggccgc ggcgggagag cgtccaaggt cgaggtccgc cgccacccgg ccaagctccg
     1201 cgcggagcgc ttcggggcag cgttcagcga cccgctggat tccaaggtgc gggttgagcc
     1261 cggcgaggag gcaggcgctg agcagatcga tcgctgccgg tgccgcttcc gccagcgctg
     1321 cgcgctgtcg ctgtttggcc cgtctgcgag tgacgcgcac cgcgagaagg accgtcgccc
     1381 cgccgagggg aatcgccagg ctgacgacgc tcccggcgcg gaacgccatg gcggcgagca
     1441 ccagtgctgc cgctgaaccg taaatccgac ggcgggtgga ccgacgcgcg gtgccacgct
     1501 cctcgcgggg cgctggctga ggacgccgcc ggctgggtgt ggtgccggtg gtgcgtcccc
     1561 gggggtgaga gcgcggagcg tggtcgttgc cgctggcgtc caaggccgcg cgtgaccagg
     1621 tcagcggatc ggggcgccga cggcggcgcg accgcctggc gggctccgag ccttgcacgg
     1681 ctgcgctcgg cggcgcgctc ggcggcgcgc tcgatggtgc cctcatcggc gggcctggcg
     1741 gcttgccggc cggttcactc ggcgaggtcg atgctcgagc gcgcaaacgt cgcgccaggt
     1801 gtgccggtgg aggtacgacg ggaggcaccg tcgggtgggc ttccggaagg ctggccgagg
     1861 gagttgacgg gtgcgcgttc ggcggtgctg ccgtgcgctc aactcgccgc tccgtaggcc
     1921 ttctgacggg tcgtcgcgag gggccggcca gcgggcgctg gttcggccgc tgcggggcgg
     1981 actcgttcgg agcgcttctt gggatgatcc gttcgggagt atcgctgggc tgagtgtcgc
     2041 gcatgcccag gacgaccgtc gcgtatgggg tgcgccgacg cgctgcctcc aagtcagcag
     2101 cccattccca atcccagtcg gctggcgtgg cgttgccgtc tgaccggcgt cgtctgggcc
     2161 ggcgatgttg ggtttgacgc ggacgtgacg gcgccgccag cgcgagcacg gcgaggatcg
     2221 aggccgcgat catgacggcg agggcgacag cggtcatctg gtcccccgga cgatccggtc
     2281 tgcccaccag atccccgctg cctcaaaggc agtcgcggcg gcgagggtcg cccagccgaa
     2341 acggccccgc acgaggaagg tcacgtcggc tggtgaaatc agggcgagca gggcgaggaa
     2401 ggcaggcgcc atggcggcga ccacgcgggc tgagagtctc gcctgggcgg tcgcggccag
     2461 cgtggcctgg gtcgcgcgta ggcggtcgct ggccgcggcc gccacgcccg cgaggacccg
     2521 agcggggggt gcgccggtcg cctcggcgag gtcgagcgcc tcgccggcaa ggaccagttc
     2581 ggccaggggt tccttgcggc caacggcggc gagtgcctgg ccggcgcccg cgccaaactc
     2641 ggccgcgcgg accgcctcgg cgagcaggcg ctgcaacggc ggcggcgcga cctccgcggc
     2701 agcctgtaac gcacgtcgcg gggcggctcc ggcgaccacg gtggcccgta ggacgtcgag
     2761 gacctcgggc acggctgcct ccagccgctc gcggcgccga cggccctggc cgggcagccg
     2821 ccgcgtgaca atggctccca cgcggctgag aagcccgctt gcggccggcg ggtccaaggc
     2881 accggtcacc ggtggcctcc ccggcgagcg gcgaggaccc ggacccgacc tgcgggagaa
     2941 gatggttcga acgcgtcgag gaccgctggg tcgaggcggt cagcccagga gggcacgtag
     3001 ccggtggcgt cgaggccctc tggaccggcc acccgaacga gcttggcctg cgcctggcca
     3061 tagtggtcga gggtcaaggc ggccaccgcg gtgacccgcc ggccggtggg ggttcgctca
     3121 aggtgcacaa ccgcctcgat gccgccggcg atgaggcggc gaaccacttc gagcaccgcg
     3181 ccgggagcgg acagcgccaa ggcttcgagg cgggcaggca cttcggcggg tccagcggcg
     3241 tggagagtgg cgagcagacc gcggtggccg gtgtttgctg cctgcagcag gtccatgacc
     3301 tcagggccgc gggcttcgcc gacgacgatt cgatcgggac gcatgcggag cgcgttgcgg
     3361 accaactctc ggagggttac ctcgccccgg tcttcgacgt tggacgggcg ggcttcgagg
     3421 cggacgacgt gggggcggtt cagggagagc tcagcggcgt cctcgatggt gaccacccgc
     3481 tcggagggtg gaatcgctgc ggccaggacg ttgagcaggg ttgtcttgcc ggtcccggtc
     3541 gctcccgagg ccaccgtggc gatgccggcg ctgaccatgg cctcaagcag ccgtgcggtg
     3601 gtggcgtcga gggtgccgcg gtcgaccagg tcggcgaggg tgaggcgctg gcgggcgaag
     3661 gtgcggatgg tcaccagcgg gccgtcgggt gccagcggtg gcaacacggc gtggaatcgt
     3721 tccccgccgg gaaggcgggc gtcgacccag ggtcgtgcct cgtcgacgcg caggccgagc
     3781 ggagcgacca cccgctggat cagtgcggcg agcgcggcgc tggaggcgag gtggatcgcg
     3841 gtgcgctcga tgcggccgtt tcgctcgacc cagacgtcgt cgggtccgtt gatcatgatg
     3901 tcggtgaccg ctgggtcggc aagcagcggg ctgatcggac cgagcccggt cagctcgtcg
     3961 gtgacggctt ggaccgtcgt ggcgaagctc gacgctggga ggacgatgcc ctcgtccctg
     4021 agcgtgtcgg cgacgacgac gcggaggtcg gcgccgctgg cgctctggag gccgccgctc
     4081 aggccgctca ggcgatgctg gacacgttcg gccaaggcgg cgactgaggc ggcggagaca
     4141 gggaccggaa agggcggagc ggcggcgtcc cctggccagg ccgcctgcca tccgtcctgc
     4201 caatcgtctt gccagggggt gtcaaagcgt acggcaggcg gttcggcggg ctgatcgaac
     4261 tccatggtgg tgctgaactc ggggtcggcg cgccagacag gtggggtcag gcgaggccat
     4321 gcggttcggc gccctcgggc cggaacggtt ctgggaggca cgtcaggggc gaggtcggtg
     4381 ctgggatgcg cgcccggatg ggggcccgga cgcaggctgg tcgatccgcg gctcatccgg
     4441 ccaccgccgg gaacgggacg acgcggttgg cgagcgtgag catcgcctgg acggcgggga
     4501 gctcctcacg gtccagcagg agggtggcct cctctgcggc ggtggacagc tcccgaggcg
     4561 ccgctgggat cgccgcccag aggcggtcgt ggaggtcctt gcgagcgtct ttcggcgcca
     4621 gcggcgcacg gctgcggacg ccggtgagga cggcaccggc ggtgctgggg tcgcggtcga
     4681 gcgcgctcca tccttcgagc gcggccagga ggccttggac tccggcggat tcgcagcgac
     4741 cgacggcgag caggacgtcc gcgagttcca gcgccgtctc gaacatgaag ctgttcgtgg
     4801 gcggcaggtc gacgacgacg acccggtaga ccgagcgggc cagatcgaag atcgcctgga
     4861 tgaggcatgg gtccggcggc ggctcgggtc ccccgtcgat caggccggcc aggggcagca
     4921 ccgagaggcc gggccacggt tcggccagcg cggcagccaa gccctccccg tcccattcca
     4981 gcgcgtgacg cggacacgcc ggacatgggc caagcagggc ttccggttcc gtctctgtcg
     5041 gcggcgctgg agcgtggaat ggcgtggcgc cgttgtccgg ctgcggcgcg tgcagcccgg
     5101 aagttgcggt caggtcggac agcccaaccg ccgccgccac ctccaggtcg catggttgga
     5161 agcccggggc tggcgcgagg tctgaccagg ttggcgcggc gggagagatg tcgagccggt
     5221 agccgaggcc gccggccaga tcggcgagca ggatcggcgc ggggccgaag cgtgaccagg
     5281 cgccggccag gtgggcggcg aggacggcga tcaccggggc ggtgaagtgc tccctgacgg
     5341 ccttggacgg gcgggtttcg gcggcgatgc gggcgagctc ggcggggagg tcggcggcgc
     5401 cgtcgggcca gggaagcagg ccacgggcgc cgacggcgag tgcggcgcgg aggtcggcgg
     5461 ggcggctgtc gtcgccgatg acgaggaggg gcacgcggca gccggcgggc agctcagcga
     5521 gcgcgccaca gccgacgagc gcgagggcgg gactggcggc gagcgcggcg ctgagcccgc
     5581 cggggctctg atctgcaacg gtggcgcgcc atccggcagc ggtttcgacc gcgtggcgca
     5641 gcgattcggc agtgcctggg tcctcgtggg cgatgacgac gagtgggagg ggggcgcggg
     5701 cggggtgtag ggccattggt ctctcctgtc ctggcgtcaa aggcttggtg ggccggccca
     5761 ccgggccggg gggcggctcc ggtgggccgg cctcgccagg atggaggata ttcgaatcaa
     5821 acgaaagact tcgaatgcaa actggggata acccttggaa gcaatccctg actgtcgcgt
     5881 cgatgccatt ggggtccctt gagtcgggtc cctgacagga tcggtttttg gacaagcggg
     5941 taagatgcgt ggaaccgggt acgactgggg gttgcggaag tggcgcggtc gccgtgaacc
     6001 gtgaggggtt gtaaccagtc gttcgcctgg acggtccaat agccgatacc cgtccgatgc
     6061 cccgtcgtca acttctcccc cgacagcgtc tcgtcgaacc tgacaccgac gtcgatgtcg
     6121 gatgcaagcc tgtggctcgt tgaggtggcg agccgaacaa cgaggtgcca gaagccagat
     6181 gactgttgcg ttcgttttcc caggtcaggg cagccaggcc ccgaagatgg gcctggactg
     6241 ggccgatgac ggctccggcg atctctatgg ctgggcgagc gcgtgtcttg gctgggacct
     6301 ggccgagacg ctggcgcacg ccacgccgga cgagctgcgc cagacctaca tcgcccaacc
     6361 tgccatcttc tgcgtttcgg tggccgccct ccaggcgctg gagcgggcgg gggtgcggcc
     6421 ctcgttcgtg gccgggcact ccctcggtga gttctcggcg ctggtcgcgg ccggggtgct
     6481 gtcgttcgag gccgggctcg ccctggtggc gcgccgggcc gaggcgatgc agcgggccgc
     6541 cgacgcccgg cccggctcga tgagctcggt gctcggcctg tccgtcgagg gcgttgagat
     6601 ggccgtcgcc gcggctgccg gcaacaacgt cctggcgatc gccaacgaca acgcgcccgg
     6661 caacgtggtg gtgtccggcg acttccccgc gctggaacgg ctgcccgtgg cggccaagga
     6721 gctgggcgcc aagcgggtgg tgccgctgaa cgtgggcggg gcgttccact cgccgctgat
     6781 ggggccggcg gcggaagcgt tctggccctt cctggcggcg gcgcccctgc gggacgcggc
     6841 cgtcccggtg gtggccaacg cgaccgccga gccggtcacg gtcgcggccg agctgcgcga
     6901 actgctcggc cggcagctca ccggaccggt ccgctggacc gagtcagtgc ggcggctggc
     6961 cgacctcggc gtcgacacgt tcgtcgaggt gggccccggc acagtgctgt ccggcctgat
     7021 caagcggacg ctcgatggtg tacgggtgct gtccgctggg gacgccgcgg gggtcgcggc
     7081 ggtggccgag acgctgcggg agcagcccga cagcccgcag gcaagcgccg ccagtgacgg
     7141 agaggaccag ggtgaggtgg tggtgcttcc ggaacggttc gcggtgtcgc cgggtcatgg
     7201 ccgcttctat ccggcggccc cgtcccgctt caccgacgag ggggcctatg tggagcctgg
     7261 cgacctgctc ggcgaggtcc gcaacgggaa cgcgagcatc cccgttcgca gcccgttccg
     7321 cggctgggtg atggctcatc tggcctggga gggcgagctg gtcacccccg gtcagctgct
     7381 gctcagcctg cggcccttct gacgttcgct tgaaggccgc tctggcaggg tttcgccgcc
     7441 gggacgttta cggaagtt
//
LOCUS       DASWDC010000056         3043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1597445, whole genome shotgun sequence.
ACCESSION   DASWDC010000056 DASWDC010000000
VERSION     DASWDC010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597445"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VF995_01830"
     CDS             <1..607
                     /locus_tag="VF995_01830"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9342340.1"
                     /translation="AGGGRVLDAGAGTGFLSVLAARLGYQVTALDLSPGMLERLRAAA
                     SREGLAIECVEGGADQPPPGPFDAVIERHLLWTLPDPGLALAAWRAVAPDGRLVLFEG
                     MWGNADPVEAVRHRARELLRRLRGEPDHHHDHYDPAVLAQLPLSGGVTPDQLVSLVER
                     AGWGIIRLERLRDVEWIQQLSLPLSQRLLGVTPRFVIRAGS"
     gene            complement(623..1480)
                     /locus_tag="VF995_01835"
     CDS             complement(623..1480)
                     /locus_tag="VF995_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX9342341.1"
                     /translation="MGVSVRFGARQVGRVALVASGAAMGTLMAEVWAATRHRFLPATQ
                     PHEIDCVLGPEDGEPLVLAVLGDSSIAGVGADSPETTLTYGVAKVMAERYRVTLHALG
                     VSGARLANVVTEQLPQLAEIDPDVVLVCVGTNDITHCTPMLEVRRQLRLLANGLETVA
                     PDAVKVVSGLPPAETSRAFRWPLRNVLGTWARAYTRLYKAELEPHDITVLDIAKVTRA
                     AFHKRPEMFSDDWFHPSSTGYAFLGTLYGSAVREALDTARERGAAALPTATAAEDEPG
                     AALAGDLAG"
     gene            complement(1599..2081)
                     /locus_tag="VF995_01840"
     CDS             complement(1599..2081)
                     /locus_tag="VF995_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342342.1"
                     /translation="MTEDPFTGRLQSEPLVGLRALREALREEERQVSYWRRLVQGRLD
                     LARTALAGGEPTAEFLAITAATRERPSETRARRSPAFTGSLRGRESPPVGDLGRLWET
                     PMPWDDTEQLTAMTNALAEAETALSLYRRTLHQRIDACTAELVGRYRRDLKQLDRIVP
                     "
     gene            2201..>3043
                     /gene="nth"
                     /locus_tag="VF995_01845"
     CDS             2201..>3043
                     /gene="nth"
                     /locus_tag="VF995_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX9342343.1"
                     /translation="MPSSTPKSGPESGPESGPRSGQEPEPGTAADVAPAATRISRRES
                     KAAKAARCGEIQARLAERYPDVTTALDHGSAWELLVATILSAQSTDVTINKIAPELFR
                     RYPTVADLAGADRGELEEVLHPTGFFRNKAKSVQGAAAYLLEHHGGEVPDRMEDLVKL
                     PGVGRKTANVVLGSWFARNEGVVVDTHVGRLSRRLGLTRESDPVKVERDLMRLLPQAD
                     WTVFSHRLIWFGREVCAAKDPRCDRCELADLCPSAFKVGRWIEKAPAKAAKAAKAAKP
                     TATAQ"
BASE COUNT          416 a         1081 c         1110 g          436 t
ORIGIN      
        1 ggccggtggg gggcgggtac tcgacgcggg tgcgggcacc gggttcctgt cggtgctggc
       61 ggcgcggctc ggctaccagg tgacagcgct ggacctgtcg ccggggatgc tggaacggct
      121 gcgcgccgcc gccagccgtg agggtctggc gatcgagtgc gtcgagggcg gcgccgacca
      181 gccgccgccg ggcccgttcg acgccgtgat cgagcggcac ctgctgtgga ccctccccga
      241 cccgggcctg gccctggccg cctggcgtgc ggtggcgcca gacggccgcc tggtgctgtt
      301 cgagggcatg tggggcaacg ccgacccggt cgaggccgtc cgccaccgcg cccgcgagtt
      361 gctgcgccgc ctccgcggcg agccagacca ccaccacgac cactacgacc ccgcggtcct
      421 cgcccagctt cccctctccg gcggcgtcac ccccgaccag ctcgtcagcc tggtcgaacg
      481 cgccggctgg ggaatcatcc gcctggagcg cctccgcgac gtcgagtgga tccagcagct
      541 ctcgctcccg ctgagccagc gcctcctcgg cgtgacccct cgcttcgtga tccgcgccgg
      601 gagctgagcc ccggtcgggg tgctacccag ccaggtcgcc cgcgagcgcg gcacccggct
      661 cgtcctcggc ggctgtggcc gtggggaggg cggcggcgcc gcgctcgcgg gcggtgtcga
      721 gggcctcgcg gacggcgctg ccgtagaggg tgccgaggaa ggcgtagccg gtgctggagg
      781 ggtggaacca gtcgtcgctg aacatctccg ggcgcttgtg gaaggcggca cgggtgacct
      841 tggcgatgtc caggacggtg atgtcgtggg gttccagttc ggccttgtag aggcgggtgt
      901 aggcgcgtgc ccaggtgccg aggacgttgc ggagcggcca gcggaaggcg cgcgaggtct
      961 cggccggcgg taagccggag acgaccttga cggcgtccgg ggccacggtc tcaaggccgt
     1021 tggcgagcag ccggagctgg cggcggacct ccagcatcgg ggtgcaatgg gtgatgtcgt
     1081 tggtgccgac gcagaccagc accacgtccg ggtcgatctc ggcgagctgc gggagctgct
     1141 cggtgaccac gttggcgagc cgcgcgccgg agacgccgag cgcgtgcagc gtgacccggt
     1201 accgctcggc catgaccttc gcgaccccat aggtcagggt cgtctcgggc gagtcggcac
     1261 cgacccccgc gatcgagctg tcacccagca ccgcgaggac cagcggctcc ccgtcctcgg
     1321 ggccgagcac gcagtcgatc tcgtgcggct gggtcgctgg caggaagcgg tgccgggtcg
     1381 ccgcccacac ctctgccatc agcgtgccca tggccgcgcc gctcgcgacc agcgccactc
     1441 gcccgacctg gcgggcaccg aaccggacgc ttactcccat cccactgcac ctcccttaca
     1501 gcagagcgta cggaggaacg ccccgtttgt gaagggaggt gcaattactg tttggaaacg
     1561 cctccgcaac cgggcggcga cgtttcctca gcaggctcct atggcacgat tcggtcaagc
     1621 tgcttcaggt cgcgccggta ccgcccgacc agctcagcgg tgcaggcgtc aatgcgctgg
     1681 tgcagggtgc ggcggtagag cgacagcgcc gtctcggcct cggccagagc attggtcatc
     1741 gcggtgagct gctcggtgtc gtcccagggc atcggggtct cccacagccg gccgaggtcg
     1801 ccgaccggcg ggctctcgcg gccccggagg ctgccggtga acgcgggtga gcggcgggcc
     1861 cgggtttcgc tgggccgctc gcgagtggcg gcggtgatcg ccaggaactc ggcggtcggc
     1921 tcgccgccgg ccaaggcggt gcgggccagg tcgagccggc cctggaccag gcggcgccaa
     1981 tagctcacct ggcgttcctc ctcgcgcagc gcctcgcgca acgcccgcag gccaaccagc
     2041 ggttcgctct gcagccgtcc ggtgaaggga tcctcggtca cggcggctcc tcttcctcgt
     2101 tcctcataac ggtccggacg tgatcgtccg cttcacttct cggcagccgg cgcgccctac
     2161 tcaagcggca ctggtggcac tggttcgata ggatcgggcc atgccgtcct ccaccccgaa
     2221 gtccggtccg gaatccggtc cggaatccgg tccgcgatcc ggtcaggaac ccgaacccgg
     2281 gacggccgcc gacgtcgcac ccgccgccac ccggatcagc cggcgcgagt ccaaggccgc
     2341 caaggccgcc cgttgcggcg agatccaggc acgactggct gagcgctacc cggacgtgac
     2401 caccgcgctc gaccacggct cggcgtggga attgctggtg gcgacgatcc tctcggcaca
     2461 gtcgaccgac gtgaccatca acaagatcgc cccggagctg ttccgccgct acccgaccgt
     2521 cgccgacctg gccggcgccg accggggaga gctggaggag gtcctgcacc caaccggctt
     2581 cttccgcaac aaggccaagt cggtccaggg cgccgccgcg tatctgcttg aacatcacgg
     2641 tggtgaggtg cccgaccgga tggaggacct ggtcaagctc ccgggggtgg gccgcaagac
     2701 cgccaatgtg gtgctcggct cctggttcgc ccgcaacgag ggcgtggtgg tcgacaccca
     2761 cgtcgggcgg ctgtcgcgcc gcctcggcct gacccgggag agcgacccgg tcaaggtcga
     2821 gcgggacctg atgcggctgc tcccgcaggc ggactggacg gtcttctccc accggctgat
     2881 ctggttcggc cgcgaggtgt gcgccgccaa ggacccccgc tgtgaccgct gcgagctggc
     2941 cgacctgtgc ccgtcagcct tcaaggtcgg ccgctggatc gagaaggccc cggcgaaggc
     3001 ggcgaaggcg gcgaaggcgg cgaagccaac ggcaaccgcg cag
//
LOCUS       DASWDC010000057        16091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_423787, whole genome shotgun sequence.
ACCESSION   DASWDC010000057 DASWDC010000000
VERSION     DASWDC010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16091
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423787"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1627
                     /locus_tag="VF995_01850"
     CDS             278..1627
                     /locus_tag="VF995_01850"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX9342344.1"
                     /translation="MDTIVNLAKRRGFVFPSSEIYGGLRSSYDYGPLGVELKNNLKRA
                     WWRSVVQLRDDVVGQDAAIIMAPKVWEASGHLESFSDPLVECTNCHQRFREDHLLEAF
                     EAKHGRPPAAGELHCPNCGKGPFTDPRNFNLMFKTFMGPVEDAAAVVWLRPETAQGIF
                     ADFPNIQQISRKKIPFGIAQVGKAFRNEITPGNFIFRTREFEQMELEFFVEPGSDEGW
                     HEQWIEERRQWYLDLGLRDDYLRLREHGADELAHYAKRAVDIEYKFPFGWSELEGIAN
                     RSDFDLSQHQKFSGQDLTYFDQEANSRFIPYVIEPSGGVDRAALAFLVDAYREEEAPT
                     ASGGTEKRTLLRLDWRLAPMKVAVLPLSRHEQLVPLAREVASSLRDRWMIDYDDARSI
                     GRRYRRQDEVGTPYCVTVDFDSLEDKAVTVRDRDTMRQDRVGLDRLVAYLDDRLPRT"
     gene            1640..2578
                     /locus_tag="VF995_01855"
     CDS             1640..2578
                     /locus_tag="VF995_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX9342345.1"
                     /translation="MASHPQPQPSPAEALPPPAGGKPAGPTAFGPRSAVLAVIGVGPD
                     GSSVDALDPLDLALLRGEAVVEAIRVYQGRPFRLAAHLARLVRSAAALELTLPRGLEA
                     LCSRAIDQAGGGDGILRLLCTRGIEGELASTVFAIITEVPPKLEAHRARGLNLVLLTV
                     AADPLVRAASPWLLPGAKSTSYAVNMAASRAAAARGADDAVFVGLGGELLELPTSNIW
                     WRAGETLYTPSLDLGILAGVNRAALQELAPSLGYRVLEGVFTADDLAAADEAFVSSTI
                     REVMPVVSIDHQPVADGRPGPAATKLHTALRRLATA"
     gene            2687..3811
                     /locus_tag="VF995_01860"
     CDS             2687..3811
                     /locus_tag="VF995_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9342346.1"
                     /translation="MRLPSALRRLRWWRWVVLLAAVAVLVALPALAGALPVPGTGRAL
                     PAATLLAKVQGSAAVPYQGYAESRAGLGLPDVPNASGVVALLGETTRMRAWVASPSSW
                     RVDQLTTIGERDLYHDADGTWLWDSGSRDAEETRGEPAVRFVRPADLLPPELGRRLAA
                     AATPAEATRIGGRRIAGVDAAGLRITPRSADTTIARVDLWADPASGLPVRVEVATKGA
                     STPILVSTFLDLRQAAPSPAVAGFQLPADANVEHVDSPDIAQAIDRWSPYVLPDQVGG
                     LPRRTTVAQAAATYGQRFDLVAALAFPDQISWRTRNFLAQLPQRSGPWGQAGMISTPL
                     LNGMIVEQGGVAYVLAGTVTQAALERAATALVHQPLGARP"
     gene            3808..4803
                     /locus_tag="VF995_01865"
     CDS             3808..4803
                     /locus_tag="VF995_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342347.1"
                     /translation="MTQPSAVATATATASAAIRTEGLTKRFGPVTAVRGLDLEVFEGE
                     LFGFLGPNGSGKTTTVRMLLGLVFATSGRIELLGQPMPAASRAVLPEVGSLIEGPAFY
                     PHLSGRANLTLFDQAGPPGAGGGRATRRRRVGEALERVGLATVGRRPVRAYSTGMRQR
                     LGLAAALLRAPRLLVLDEPTNGLDPQGMREVRDLLAGLVAGGTTVFLSSHLLAEVELI
                     CTRAAIVHQGRLVAQDRVDALLVPTGRVEVATPDLDRATALAGRLPGIGVGEPRAGRL
                     VVQLDGTSPEALNQQLVEGGVRVRELVMVRPSLEDVFLALTGTRTGEREAPDAAG"
     gene            4790..5593
                     /locus_tag="VF995_01870"
     CDS             4790..5593
                     /locus_tag="VF995_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9342348.1"
                     /translation="MLRVELRKLVRRPRTWVSIALLAGLPTLVAVFLKVTGIGPRPGE
                     GPAFLAEVLNNGALFPAAALALVLPLFLPVAVSVTAGDAVAGEASTGTLRYLLTRPVG
                     RTRLLVVKLIVTILFVFLAVVIVAAVAFVVGATMFGVKPLPSVSGQTIAGPEASWRTT
                     VTVIYVAWSMLGVASIALFASTVTDSPLAAALSALAALVTSQVLDLLDAAASVKPYLP
                     THYWLSFIDLFRTPILWHNVSRGFGLQGVYILVFLGAAWANFATKDIKS"
     gene            complement(5692..6483)
                     /locus_tag="VF995_01875"
     CDS             complement(5692..6483)
                     /locus_tag="VF995_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9342349.1"
                     /translation="MRWQEGGERVYRCRYQSFDLNIGVVVGEGEVLVIDTRSWRAEGE
                     QLREDLRRLTPLPCRQVVNTHMHFDHSFGNASFRGAAIWGHPRCAELLRTRGEEQRAQ
                     ALAWADQDDAVAEIRATEIVPPDRLAAGETVLSVGGRAVELRHLGRGHTDNDLVVVVP
                     DAGVLFAGDLVEEGAPPLFEDAFPLEWPGTVERLLAIAPPVIVPGHGDVVAPEFARRQ
                     RDELAEIASLCRQLLSGELSFDEALRRAPYPDAAARQALNRAAAP"
     gene            complement(6480..7295)
                     /locus_tag="VF995_01880"
     CDS             complement(6480..7295)
                     /locus_tag="VF995_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEX9342350.1"
                     /translation="MFSFSSLSPRRRLLLGGLALLVAVATAVSLLFAVVLRPGPSRAP
                     RSRIVPVLLVPGYGGTPSSLNILAARLRGAGRQVSVVGLPDDGTGEIGASAVVLGAAV
                     ARTMAATGADHVDLVGYSAGGLVVRAFLALPGGAGHTRHVVLLGSPNHGALLASVATL
                     LGGQVCSGACPELAPDSAFLQRLNGTREVPAGPDFTSIWTADDRIVEPPASAVLAGAR
                     NIRLQDICASASVDHGGLVRDPLPLALVGRALAGALPAQLGPSDCVTLREGGR"
     gene            7386..10106
                     /gene="ppdK"
                     /locus_tag="VF995_01885"
     CDS             7386..10106
                     /gene="ppdK"
                     /locus_tag="VF995_01885"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX9342351.1"
                     /translation="MTKYVYDFEEGNKDMKYLLGGKGANLAEMTNMGLPVPHGFTVTC
                     EACNAYRAAGRQFPDGMLDEVDEHLRALERKMNRRLGDPGDPLLVSVRSGAPFSMPGM
                     MDTVLNVGLNDNSVDGLAKQTGSERFAWDSYRRLLQMFGKTVLGVDGDRFEDAIDHAK
                     ERKGVRNDVDLDEADLRQLVDRFKAIIREETSRDFPQEPIEQLRLGIKAVFGSWDNKR
                     AIDYRRKNKIDDSLGTAVNVQSMVFGNKGDDSGTGVAFTRNPANGDNHPYGDYLANAQ
                     GEDVVAGIRNTLALDEMGQVQPQAWELLQRHMQTLEQHYKDMCDIEFTVEQGKLWLLQ
                     TRVGKRTAFAEWVIAFDMLDEDLIDVDTAVLRLDANRLEELFKKVIAPGTKAEAIASG
                     LNASPGAAVGRAVFTADDAEAWAQRGEPVILVRRETTPDDYHGMIRSQGILTSAGGTN
                     SHAAVVARGEGIPAVCGADAIRVDRQGKKFTVGSTSVAEGDWVTIDGFSGKVYAGQLE
                     LADSPIERARSGDPEARKEKIWRAYERFMARADEVRRLRVHANADTPGQSANAIERGA
                     EGIGLCRTEHMFLGEERVQAVRKMIFADTDAEEQAAYDALLPLQRDDFVGIFQAMDGK
                     PVTIRLLDPPLHEFLPNQVDLAVAVALAKQAGERQVQLGGDETLPLADAEEMLAKVND
                     LHEANPMLGLRGVRLGIVKPGLYAMQVRAIMEAACQVKQAGGNPVVEIMIPLAATREE
                     LAQMREELEPVVHQILRDHNLELEVLFGTMIELPRAALVAGEVAEVAEFFSFGTNDLT
                     QTAFGFSRDDIGKFLGLYEERKLVPANPFVTVDRPGVGRLMRIAAEEGRATRPDLHLG
                     ICGEHGGDPDSVRFCHEIGLDYVSCSPFRVPTARLAAGQAVLGEAASASK"
     gene            10120..10722
                     /locus_tag="VF995_01890"
     CDS             10120..10722
                     /locus_tag="VF995_01890"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020119043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX9342352.1"
                     /translation="MTEAVGPSTGAAPPPPPPGPRLRLRPVRRDDLALLRRWISDPEA
                     ASFNWFGFISETRWQQDYDENGLLSDDHGNLIVEIDVATGPGGTPTPTPIGDVSYYTV
                     RYGPNTASRCHGIGIHLTPEWRGHGYGTEAQRQLAAYLFATTTIERLEAATDVDNLAE
                     QRSLEKAGFTREATIRQASFREGSWHDLALYRRLRGDQAG"
     gene            complement(10781..11356)
                     /locus_tag="VF995_01895"
     CDS             complement(10781..11356)
                     /locus_tag="VF995_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX9342353.1"
                     /translation="MTHDLLVVQVPLLEKAIRSVAVYLFLLIVLRLAGKREMAQLNNF
                     DLVVLLMLSNTVQNAIIGPDNSLTGGLLGAAILIGANRLVVHLSYDRPMLSRLVQGTP
                     TILVRNGRTLGKNLHRELISKDELLAALRHQGTKKLSDVDLAVLEPSGTLTIEQRDHL
                     EEILDRLQRIEDRLGGPPGAGTGAEPEPARP"
     gene            11439..12497
                     /locus_tag="VF995_01900"
     CDS             11439..12497
                     /locus_tag="VF995_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9342354.1"
                     /translation="MKVTQDWVRLGTVRAELEAAEARTLVPWAARSAESRGRAQPEPE
                     DSVRTCWMRDLDRIIFSRAMMRAHGKTQSFIPAFSGEGPAAGRQGGPYIGDHYVTRAT
                     HMQQTGRIAATIAQALTLNVPLAGAIATGHDLGHPCFGHGGETALQALAPDGFDHAAQ
                     GVRLLGLLEPRNLTAEVLEGIGRHSWKHEPPSTLEGMCARLADRIAYLTADLTDALRA
                     GVLGAIGDLPGPVRRVLGDNPSDMIGTLVEDVIRHSRGAPRVVQGDVCAEAMDRLRSF
                     MFERVYLRPESERQTERARRLLTDLYGHYRENPDTMPMGASLASDLVEQQAVDAIAGM
                     TDRYALAAWKVAFTTDTG"
     gene            12552..13100
                     /locus_tag="VF995_01905"
     CDS             12552..13100
                     /locus_tag="VF995_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEX9342355.1"
                     /translation="MDLEPDQKRALLRASDADREETVQLLRLHHSEGRLTIDEFTERM
                     EQAYAAKTYGDLSVLTTDLPLSVPPTPTVLDHKPPQPKAPANHRRSFRQHLVAYTWVN
                     GFMISLWALASLVSGHMLFFWPIWTILGWGAALGSHALRAFGSPSPEGGHELAYRQRQ
                     HRYDRWSARERRRRRRYYRYYR"
     gene            13253..15214
                     /locus_tag="VF995_01910"
     CDS             13253..15214
                     /locus_tag="VF995_01910"
                     /inference="COORDINATES: protein motif:HMM:NF019880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEX9342356.1"
                     /translation="MAGRIRDDSIAQIREATDIVALVGEYVSLRPAGGTRMKGLCPFH
                     QEKTPSFTVESVKGVWHCFGCQEGGDAIDFLQKQASLSFVEATERLAQRAGIELRYEG
                     RAVGERGSLGRKSRLVAAHAEAVAFYHQLLLSSPEARPARGYLSSRGFDRAAAERFKL
                     GWAPGDQWEALVTHLRGKGYGSQELIDAGLAKPGNRGLRDAFHGRVLFPIFDVAGDPV
                     AFGARILDLDDQGGSGQGGPEHGGSGRGADRGPKYLNTAETVIWRKGQVLYGLNWAKV
                     PIVQASFAVVVEGYTDVIACHRAGVTQAVATCGTALREDHFKLLARFTEKIVLAFDAD
                     TAGGNAAGRGVNELPRPDQASGGTGFAFLGSSMGQLIADRVAALTTYVLVMPAGQDPA
                     DLVVERGGAAFRALIDQAQPLVGWWLDRKLADFDLRNPEGKVRAARELVPLLRSIPDS
                     LQRTEYARSVVQRLHLDEREYLAMVAGQQAPSRVRVGEPKEPPRTRTPQLRVECEALK
                     FALQHSDWTAETAVSWEADWFTAPSTKASFAALVKAGGPGAPLEAVLEAAANETERRF
                     LRGLAVEAFAAEENRGYAGEVFRRLEEFRLTRVIDQLKGTLQRMNPVERPDEYTHLFE
                     ELIAFEARRRALREPRPEGDGDAILQRPI"
     gene            15262..>16091
                     /locus_tag="VF995_01915"
     CDS             15262..>16091
                     /locus_tag="VF995_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9342357.1"
                     /translation="MAMAKEAQIDEVRDLVAKGKERGFLTNEEVIEALAPVDVSAEQM
                     DNILQHLQDENIEVVEMVDELEAEEFAREARSARDDELALKAPTNDPVRMYLKEIGKV
                     PLLTAEQEVILAKAIEEGEAATAELDKAADSGKKLSTTRLRELQRVERQGQLGKKKLI
                     EANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQA
                     ITRAIADQARTIRIPVHMVETINKLIRIQRQLLQDLGREPTPEEIGREMEFSPEKVRE
                     "
BASE COUNT         2434 a         5979 c         5420 g         2258 t
ORIGIN      
        1 ttcccggttt ggcctcggtg cctgtggaaa ccgcgttgct ggaaggcggc cacccaggta
       61 gcgtacgcag cagcgacggc tggtcgcccc ggccgcgtcc ggcggtctgg gcggccacat
      121 ggccgcgcga acgccgcgcc acctggcgtg gcggcggttg ttccctggca ggccgcagac
      181 caccggtcaa cctggtccgc cacgcccaga cccacccctc gaccgtcccg caggccatgc
      241 ggggccccag gaggtatcgc aagtgcccgc cgacctgatg gacaccatcg tcaatttggc
      301 caagcgccgc ggcttcgtct tcccctccag cgagatctac ggtggcctgc gctccagcta
      361 cgactacggc cccttgggcg tcgaactcaa aaacaacctc aagcgggcct ggtggcgcag
      421 tgtggtgcag ctgcgcgacg acgtggtcgg ccaggacgcg gcgatcatca tggcccccaa
      481 ggtgtgggag gcctccgggc acctggagtc gttctccgac ccgctggtgg agtgcaccaa
      541 ctgccaccag cgcttccgcg aggaccacct gctggaggcg ttcgaggcca agcacggccg
      601 cccgccagcg gcgggggagc tgcactgccc caactgcggc aaggggccgt tcaccgaccc
      661 gcgcaacttc aacctgatgt tcaagacctt catgggcccg gtcgaggacg ccgccgcggt
      721 ggtctggctg cggcccgaga ccgcccaggg gatcttcgcc gatttcccga acatccagca
      781 gatcagccgc aagaagatcc ccttcgggat cgcccaggtc ggcaaggcgt tccgcaacga
      841 gatcaccccc ggcaacttca tcttccgcac ccgcgagttc gagcagatgg agctggagtt
      901 cttcgtcgaa cccggcagcg acgaaggctg gcatgagcag tggatcgagg agcgccgcca
      961 gtggtacctc gacctcggcc tgcgcgacga ctacctgcgc ctgcgcgagc acggcgccga
     1021 cgagctggcc cactacgcca aacgcgcggt cgacatcgag tacaagttcc cgttcggctg
     1081 gtcggagctt gaggggatcg ccaaccggtc ggacttcgac ctgtcccagc accagaagtt
     1141 ctccggccag gacctcacct acttcgacca ggaagccaac agccgcttca tcccctacgt
     1201 gatcgagccc tccggcgggg tcgaccgcgc cgccctggcc ttcctggtcg acgcctaccg
     1261 cgaggaggag gcacccaccg cctccggcgg caccgagaag cggaccctgc tgcggctgga
     1321 ctggcgcctg gcgccgatga aggtcgcggt gctgccgctg tcgcgccatg agcagctggt
     1381 gccgctggcc cgcgaggtcg cctcctcgct gcgggaccgc tggatgatcg actacgacga
     1441 cgcccgttcg atcgggcggc gctaccgccg ccaggacgag gtcggcacgc cctactgcgt
     1501 caccgtcgac ttcgactccc tggaggacaa ggccgtcacc gtccgcgacc gcgacaccat
     1561 gcgccaggat cgcgtcggcc tcgaccgcct cgtcgcctac ctggacgacc ggctgccgag
     1621 gacgtagggt cgcacgacca tggccagtca cccgcagccg cagccgagcc ccgccgaggc
     1681 gctcccaccg ccagccggcg ggaagccggc cgggcccacc gcgttcgggc cccggtccgc
     1741 ggtgctggcc gtgatcggcg tcggccctga cggcagctcg gtggacgccc tggacccgct
     1801 cgacctcgcc ctgctgcgcg gcgaggcggt ggtcgaggcg atccgcgtct accagggccg
     1861 cccgttccgg ctggcggcgc acctcgcccg cctggtccgc tccgccgccg cactcgaact
     1921 gaccctccca cgcggcctgg aggcgctctg cagccgcgcg atcgaccagg ccggcggcgg
     1981 cgacgggatc ctgcggctgc tctgcacccg cgggatcgag ggcgagctcg cctccaccgt
     2041 cttcgcgatc atcaccgagg tccccccgaa gctggaagcc catcgggccc gcggcctcaa
     2101 cctggtcctg ctgaccgtcg ccgccgatcc gctggtccgc gccgcctccc cctggctcct
     2161 ccccggcgcc aagagcacca gctacgcggt gaacatggcc gccagccgtg ctgccgccgc
     2221 ccgcggcgcc gacgacgcgg tctttgtcgg cctcggcggc gagctgctgg agctgcccac
     2281 ctccaacatc tggtggcggg cgggggagac cctctacacc ccctccctgg acctcggcat
     2341 cctcgccggc gtgaaccgcg ccgccctcca ggagctggcc cccagcctcg gctaccgcgt
     2401 cctcgaaggc gtcttcaccg ccgacgacct cgccgccgcc gacgaagcct tcgtctccag
     2461 caccatccgc gaggtcatgc ccgtcgtcag catcgaccac cagcccgtcg ccgacggccg
     2521 ccccggcccc gccgccacca agctccacac cgccctgcgt cgcctcgcca ccgcctgacc
     2581 gggcccagct ccgttctccg gctccgttct ccggcgcgga ttcccggtcg gatccccggc
     2641 tcggcgcact ccagcacaat cgggctggac gccctaccgt ggaaggatgc gactgccgtc
     2701 cgctctccgg cggctgcggt ggtggcgctg ggtcgtcctg ctcgccgccg tggccgtgct
     2761 ggtggcgttg ccggcgctgg ccggcgcgct gccggtgcct ggcactggcc gggcgctgcc
     2821 ggcggcgacc ctgctcgcca aggtgcaggg ctcggcggcg gtgccctacc agggctacgc
     2881 ggagtcgagg gcgggcctcg ggctacccga cgtgcccaac gccagcggtg tggtcgccct
     2941 gctcggcgag accacccgga tgcgcgcctg ggtggccagc ccctccagct ggagggtcga
     3001 ccagctcacc accatcggcg agcgcgacct ctaccacgac gcggacggga cctggctgtg
     3061 ggactctggc agccgcgacg ccgaggagac ccgcggcgag ccggcggtcc gcttcgtccg
     3121 gcccgccgac ctgctcccgc cggagctcgg ccgccggctg gcggccgccg cgacccccgc
     3181 cgaggcgacc cgtatcggcg gccgccggat cgccggggtc gacgcggccg ggctgcggat
     3241 caccccacgc tcggccgaca ccaccatcgc ccgcgtcgac ctgtgggccg acccggccag
     3301 cggcctcccc gtccgggtcg aggtcgccac caaaggcgcg tccacgccga tcctggtcag
     3361 caccttcctc gacctgcgcc aggccgcccc cagcccggcg gtggccggct tccagctgcc
     3421 cgccgacgcc aacgtcgaac acgtcgacag ccccgacatc gcccaggcga tcgaccgctg
     3481 gtcgccgtac gtgctgccgg accaggtcgg cggcctgccc cgccgcacca cggtcgccca
     3541 ggcagcggcc acctacggcc agcgcttcga cctggtcgcc gcgctcgcgt tccccgacca
     3601 gatctcctgg cgcacccgca acttcctggc acagctgccg cagcggtccg ggccgtgggg
     3661 gcaggccggc atgatctcga cgccgctgct caacggcatg atcgtggagc agggcggggt
     3721 cgcctacgtg ctcgccggga cggtcaccca ggccgccctg gaacgcgccg ccaccgccct
     3781 ggtccaccag ccgctcggcg cccgcccatg acccagccga gcgccgtcgc cactgccact
     3841 gccactgcca gcgccgcgat ccgcaccgag ggcctcacca aacggttcgg cccggtcacc
     3901 gccgtgcgag gcctcgacct ggaggtgttc gagggggagc tgttcgggtt cctcggcccc
     3961 aacgggtcgg gcaagaccac cacggtgcgg atgctgctgg ggctggtgtt cgccacctcg
     4021 ggacgaatcg agctgctcgg ccagccgatg ccggcggcct cccgcgcggt cctgccagag
     4081 gtcggctccc tgatcgaggg cccggcgttc tacccgcacc tgtccggccg cgccaacctg
     4141 accctgttcg accaggccgg ccctcccgga gcgggcggcg gccgggcgac ccggcgccgc
     4201 cgcgtcggcg aggcgctgga gcgggtcggc ctggcgaccg tcggccgccg cccggtgcgg
     4261 gcctactcga ccgggatgcg ccagcggctc gggctggccg cggcgctgct gcgcgccccg
     4321 cgcctgctgg tgctggacga gcccaccaac ggcctcgacc cgcagggcat gcgcgaggtc
     4381 cgcgacctgc tcgccgggct ggtcgccggc gggacgaccg tgttcctctc cagccacctg
     4441 ctggccgagg tggagttgat ctgcacccgc gccgccatcg tccaccaggg ccgcctggtc
     4501 gcccaggacc gcgtcgacgc cctgctggtc cccaccggca gggtcgaggt cgccaccccg
     4561 gatctcgacc gcgcgaccgc gctggccgga cggctgcccg gcatcggcgt tggcgagccg
     4621 cgcgccggcc gcctggtggt gcagctcgac ggcaccagcc ccgaggcgct caaccagcag
     4681 ctggtggagg gcggggtccg ggtccgcgag ctggtcatgg tgcgcccctc cctggaggac
     4741 gtgttcctgg ccctcaccgg cacgcgcacc ggcgagcggg aggctcccga tgctgcgggt
     4801 tgagctgcgc aagctggtcc gccgcccgcg gacctgggtg tcgatcgcgc tgctggccgg
     4861 cctgcccacc ctggtcgcgg tgttcctcaa ggtcaccggc atcggccccc gccccggcga
     4921 gggcccggcc ttcctcgccg aggtgctcaa caacggcgcg ctgttccccg ccgccgcgct
     4981 ggcgctggtg ctgccgctgt tcctgccggt ggcggtctcg gtgaccgccg gcgacgccgt
     5041 cgccggcgag gccagcacgg gcaccctgcg ctacctgctc acccgaccgg tcggccgcac
     5101 ccgcctgctg gtggtcaagc tcatcgtcac catcctgttc gtgttcctcg ccgtggtgat
     5161 cgtcgcggcg gtcgcgttcg tcgtcggcgc caccatgttc ggcgtcaagc ccctcccgtc
     5221 ggtctccggt cagaccatcg ccggcccgga ggcgagctgg cgcaccaccg tcaccgtcat
     5281 ctacgtcgcc tggtcgatgc tcggcgtcgc ctcgatcgcg ctgttcgcct ccaccgtcac
     5341 cgactcgccc ctggccgccg ccctcagcgc gctcgccgcc ctggtcacct cgcaggtcct
     5401 cgacctgctc gacgcggcag cctcggtcaa gccctacctg cccacccact actggctctc
     5461 cttcatcgac ttgttccgca cccccatcct gtggcacaac gtcagccgcg gcttcggcct
     5521 ccaaggcgtc tacatcctgg ttttcctcgg tgccgcctgg gcgaatttcg ccaccaagga
     5581 catcaagagc tgacccacag cgggtcgccg cgcccctgcc gaaatgcggc ggccacgaag
     5641 gcgacgccgc ggtccaacag gttcaaggac ggctccgggc gactgggcac gtcacggcgc
     5701 cgccgcccgg ttgagggctt ggcgggcggc ggcgtcgggg tagggggcgc ggcgcagcgc
     5761 ctcgtcgaag gacagctcgc cgctgaggag ctggcggcac agcgaggcga tctccgccag
     5821 ctcgtcgcgc tggcggcggg cgaactccgg ggcgaccacg tcgccgtggc cgggcacaat
     5881 caccggtggg gcgatcgcga gcagccgttc gacggtgcca ggccactcca gcgggaacgc
     5941 gtcctcgaac aacggtggcg cgccctcctc aacaagatcg ccggcgaaca gcacgcccgc
     6001 gtccgggacc accacgacca ggtcgttgtc ggtgtggccg cgacccaggt gacgcagctc
     6061 cacggcgcgg ccgccgaccg acaggacggt ctcgccggcg gccagccggt cgggcgggac
     6121 gatctcggtg gcgcggatct ccgccacggc gtcgtcctgg tccgcccacg cgagcgcctg
     6181 ggcgcgctgc tcctcgccgc gggtgcggag cagttcggcg cagcggggat gcccccagat
     6241 ggcggcgccg cggaagctcg cgttgccgaa gctgtggtcg aaatgcatgt gggtgttgac
     6301 cacctggcgg cagggcagcg gggtgaggcg ccgcaggtcc tcgcggagct gctcgccttc
     6361 ggcgcgccag ctgcgggtgt cgatcaccag cacctcgccc tcgccgacga ccacgccaat
     6421 gttcaggtcg aaggactggt agcgacagcg gtagacccgc tcgccgccct cctgccacct
     6481 catcgccccc cctcgcggag ggtcacgcag tcggacgggc cgagctgggc ggggagcgca
     6541 ccggccaggg cacggccgac cagggcgagc gggagcgggt cgcggaccag gccgccgtgg
     6601 tcgacgctgg cgctggcgca gatgtcctgg agccggatgt tgcgggcgcc ggcgaggacc
     6661 gcggaggccg gcggctcgac gattcggtcg tcggcggtcc agatgctggt gaaatcggga
     6721 cctgccggga cctcccgggt gccgttgagg cgctgcagga acgcggagtc cggggccagc
     6781 tcggggcagg cgccgctgca gacctgaccg ccgagcagcg tcgccacgct ggcgagcagg
     6841 gcgccgtggt tggggctgcc gagcaggacc acatggcggg tatggccggc cccgcccggg
     6901 agcgccagga acgcacgcac caccagcccg ccagccgaat agccgaccag gtcgacgtgg
     6961 tcggcgccgg tcgccgccat cgtcctggcg accgccgcgc cgagcaccac cgccgaggcc
     7021 ccgatctcgc cggtgccgtc atcgggcagg cccaccacgc tgacctgccg gccggcgccg
     7081 cgcagccggg cggccaggat gttcagggac gagggcgtgc caccgtagcc ggggaccagc
     7141 agcactggca cgatgcggct gcgcggcgcc cggctcggac ccgggcgcag gacgaccgcg
     7201 aacagcaggc tcaccgcggt cgcgaccgcg accagcagcg cgagtccccc gaggagcagc
     7261 cgccgccgtg ggctcagcga cgagaacgag aacatgaatc cattgttgtc cacagatacc
     7321 cgatctgggg cgcggcccgc gatgtgggca tataggcttg gagggagcgt tcaaagggag
     7381 gcgtcatgac caagtatgtg tatgactttg aagagggcaa caaggacatg aagtaccttc
     7441 tcggcggcaa gggagccaac ctcgccgaga tgaccaacat ggggctgccg gtcccgcatg
     7501 ggttcacggt cacctgtgag gcgtgcaacg cctaccgtgc cgccgggcgg cagttccccg
     7561 acggcatgct cgacgaggtg gacgaacacc tccgggcgct ggagcggaag atgaaccgcc
     7621 gcctcggtga tcccggcgac ccgctgctgg tgtcggtccg ctccggggcg ccgttctcga
     7681 tgcccggcat gatggacacg gtgctcaacg tcggcctcaa cgacaacagc gtcgacggcc
     7741 tcgccaagca gaccggctcc gaacgcttcg cctgggactc ctaccgccgg ctgctgcaga
     7801 tgttcggcaa gaccgtcctc ggcgtcgacg gcgaccggtt cgaggacgcc atcgaccacg
     7861 ccaaggagcg caaaggcgtc cgcaacgacg tcgacctgga cgaggccgac ctgcgccagc
     7921 tggtcgaccg cttcaaggcg atcatccgcg aggagaccag ccgcgacttc ccgcaggagc
     7981 ccatcgagca gctccgcctc ggcatcaagg cggtcttcgg ctcctgggac aacaagcgcg
     8041 cgatcgacta ccgccgcaag aacaagatcg acgactccct cggcaccgcc gtcaacgtcc
     8101 agtcgatggt cttcggcaac aagggcgacg actccggcac cggcgtcgcc ttcacccgca
     8161 accccgccaa cggcgacaac cacccctacg gcgactacct cgccaacgcc cagggcgagg
     8221 acgtcgtcgc cggcatccgc aacaccctcg ccctcgacga gatgggccag gtccagcccc
     8281 aggcctggga gctgctccag cgccacatgc agaccctgga gcagcactac aaggacatgt
     8341 gcgacatcga gttcaccgtc gagcagggca agctctggct gctgcagacg cgcgtcggca
     8401 agcgcaccgc cttcgccgaa tgggtgatcg ccttcgacat gctcgacgag gacctgatcg
     8461 acgtcgacac cgccgtgctg cgcctggacg ccaaccggct ggaggagctg ttcaagaagg
     8521 tcatcgcccc cgggaccaaa gccgaggcga tcgccagcgg cctcaacgcc tccccgggcg
     8581 ctgccgtcgg ccgggcggtg ttcaccgccg acgacgccga agcgtgggcg cagcgcggcg
     8641 agccggtgat cctcgtccgc cgcgagacca cccccgacga ctaccacggc atgatccgct
     8701 cccagggcat cctcacctcc gccggcggca ccaactccca cgccgccgtg gtcgcccgcg
     8761 gcgagggcat cccggcggtc tgcggcgccg acgccatccg ggtcgaccgc caaggcaaga
     8821 agttcacagt gggttcgacc agcgtcgccg aaggcgactg ggtcaccatc gacggcttca
     8881 gcggcaaggt ctacgccggc cagctggaac tcgccgactc cccgatcgag cgggcccgct
     8941 ccggcgaccc cgaagcacgc aaggagaaga tctggcgtgc ctacgagcgc ttcatggccc
     9001 gcgccgacga ggtgcgccgc ctcagggtcc acgccaacgc cgacaccccc ggccagtccg
     9061 ccaacgccat cgaacgcggc gccgaaggca tcggcctgtg ccgcaccgag cacatgttcc
     9121 tcggtgagga acgggtacag gcggtccgca agatgatctt cgccgacacc gacgccgagg
     9181 aacaggccgc ctacgacgcg ctgctgccgc tgcagcgcga cgacttcgtc ggcatcttcc
     9241 aggcgatgga cggcaagccc gtcaccatcc gcctgctcga cccgccgctg cacgagttcc
     9301 tgcccaacca ggtcgacctc gccgtcgcgg tcgcgctcgc caagcaggcg ggcgagcgac
     9361 aggtccagct cggcggcgac gagaccctcc cgctcgccga cgccgaggag atgctcgcca
     9421 aggtcaacga cctgcacgag gccaacccca tgctcggtct gcgcggcgtc cgcctcggca
     9481 tcgtcaagcc cggcctgtac gccatgcagg tccgcgccat catggaggcc gcctgccagg
     9541 tcaagcaggc cggcggcaac ccggtcgtcg agatcatgat cccgctggcc gccacccgcg
     9601 aggaactcgc ccagatgcgc gaggaactcg aaccggtcgt ccaccagatc ctgcgcgacc
     9661 acaacctcga gctggaggtc ctgttcggca ccatgatcga gctgccccgc gccgccctgg
     9721 tcgccggcga ggtcgccgag gtcgccgagt tcttctcctt cggcaccaac gacctcaccc
     9781 agaccgcgtt cgggttctcc cgcgacgaca tcggcaagtt cctcggcctc tacgaggaac
     9841 gcaaactggt ccccgccaac ccgttcgtca ccgtcgaccg ccccggcgtc ggccgcctca
     9901 tgcgcatcgc cgccgaggag ggccgcgcca cccgcccgga cctccacctc ggcatctgcg
     9961 gcgagcacgg cggcgacccc gactcggtcc gcttctgcca cgagatcggc ctcgactacg
    10021 tgtcctgctc acccttccgc gtgccgaccg cccgcctggc cgccggccaa gccgtcctcg
    10081 gcgaagccgc cagcgccagc aagtaaccag ccgccaccga tgaccgaagc cgtggggccg
    10141 tccaccgggg cggccccacc cccaccacca ccgggacccc gcctccggct ccggccagtc
    10201 cggcgcgacg acctcgcgct gctgcgccgc tggatcagcg atcccgaagc cgccagcttc
    10261 aactggttcg gcttcatcag cgagacccgc tggcagcagg actacgacga gaacggcctg
    10321 ctcagcgacg accacggcaa cctcatcgtc gagatcgacg tcgccaccgg accaggcggc
    10381 acgccgacgc ccacgccgat cggcgacgtc agctactaca ccgtccgcta cggccccaac
    10441 accgcctcac gctgccacgg gatcggcatc cacctcaccc ccgaatggcg cggccacggt
    10501 tacggcaccg aagcacagcg ccagctcgcc gcctacctgt tcgccaccac caccatcgag
    10561 cgcctcgaag ccgccaccga cgtcgacaac ctcgccgagc aacgctccct cgaaaaagcc
    10621 ggcttcaccc gcgaagccac catccgccag gcctccttcc gcgagggcag ctggcacgac
    10681 ctcgccctct accgccgtct ccgcggcgac caggccggct gaccgcaccc ggcgaacacc
    10741 tcaccacctc accgacctca cgccgccggc cgcacggccc ctacggcctc gccggttccg
    10801 gctccgctcc cgtccccgcc cccggcggcc cgccgaggcg atcctcgatc cgctgcagcc
    10861 ggtcgaggat ctcctccagg tggtcgcgct gctcgatggt cagggtgccg ctcggctcca
    10921 gcaccgcgag gtccacgtcg gaaagcttct tggtgccctg atggcgcagc gccgccaaca
    10981 gctcgtcctt ggagatcagc tcgcggtgaa gattcttccc cagggtgcgg ccgttgcgga
    11041 ccaggatcgt cggcgtcccc tgcaccagcc tgctcagcat cggccggtca tagctcaagt
    11101 ggaccaccag ccggttcgcc ccgatcagga tggcagcgcc caacagacca ccggtcaggg
    11161 agttgtcggg gccgatgatg gcgttctgga ccgtattgga caacatcagc agcacgacca
    11221 gatcgaagtt gttcaactgg gccatttcgc gtttgccggc gaggcggagg acgatcagca
    11281 ggaacaggta gacggcgact gagcggatcg ccttttcgag caggggtacc tggacgacga
    11341 gcaggtcgtg ggtcatggct gtctcctgtc ggctggttgt cgcaggccag ggtcacactt
    11401 gtcgggatgg gtcgttcgga ctgtaacggg agcacgacgt gaaggtgacg caggactggg
    11461 tgcggcttgg gaccgtgcgg gcggagctgg aggcggctga ggcgcgcacg ctggttccgt
    11521 gggcggcgcg gtcggccgag tcccggggca gggcgcagcc ggagccggag gactcggtgc
    11581 gcacctgctg gatgcgggac ctggaccgga tcatcttctc gcgggcgatg atgcgcgccc
    11641 acggcaagac ccagtcgttc atcccggcgt tctccggtga gggtccggcc gctggccgcc
    11701 agggcgggcc ctacatcggc gaccattacg tcacccgcgc cacccacatg cagcagactg
    11761 ggcgcatcgc tgccacgatc gcgcaggcgc tcaccctcaa tgtgccactg gcgggcgcga
    11821 tcgcgaccgg tcacgacctt ggccatccct gcttcggcca tgggggcgag acggccctcc
    11881 aggcgctggc gccggacggg ttcgaccacg ccgcacaggg cgtgcggctg ctcggcctgc
    11941 tggagccccg gaacctgacc gccgaggtgc tggagggcat cggtcgccat tcgtggaagc
    12001 acgagccgcc ctccacgctg gaggggatgt gcgcccggct ggccgatcgg atcgcctacc
    12061 tgaccgccga cctgaccgac gcgctgcggg cgggggtgct cggcgccatc ggcgacctgc
    12121 ccggcccggt ccggcgggtg ctcggcgaca acccctccga catgatcggc acgctggtcg
    12181 aggacgtgat ccgccactcc cgcggcgctc cgcgggtggt ccagggcgac gtctgcgccg
    12241 aggcgatgga tcgtcttcgc tcgttcatgt tcgagcgggt ctacctgcgg cccgagtcgg
    12301 agcggcagac cgagcgggcg cgccgtttac tgaccgacct gtacgggcat taccgggaga
    12361 acccggacac gatgccgatg ggtgcgagtc tcgcaagcga cctggtcgaa cagcaggcgg
    12421 tcgacgccat cgccgggatg accgaccgtt acgcgcttgc cgcttggaag gtcgccttca
    12481 cgactgatac cggctaggac tgactgctac tctggacttt ggtcgagggg tggtgagcag
    12541 tgggaggcct gatggacctc gagccggacc agaaacgcgc gttgctgcgc gcctccgacg
    12601 ccgaccgtga ggaaacggtc cagctcctgc gcctccacca cagcgagggt cgcctcacca
    12661 tcgacgagtt caccgagcgc atggagcagg cctacgcggc caagacctat ggcgacctga
    12721 gcgtcctgac caccgacctg ccgctgtcgg tcccgccgac ccccacggtc ctcgaccaca
    12781 agccgccgca gccgaaggcg cccgccaacc accggcgcag ctttcgccag catctggtgg
    12841 cctacacctg ggtgaacggc ttcatgatca gcctgtgggc gttggccagc ctggtcagcg
    12901 gccatatgtt gttcttctgg ccgatctgga cgatcctcgg ctggggggcc gcgcttggct
    12961 cccacgcgct gcgcgcgttc ggttcgccct ctccggaggg cggccacgag ctcgcctacc
    13021 ggcagcggca gcatcgctac gatcgctggt ccgcccgtga acgacggcgg cggcgtcgct
    13081 actaccgcta ctaccgatga cgcggtagcg cggcttcagg acttctccac aggttcggct
    13141 tcgggctggc cggcgaccat cgggtgtcgg gtgcgcaacg tacaatcgct ccgcgccctg
    13201 tccctcccca cagcgcgctt ggtattgatc ccatcgaggt gcagggggcg tcatggctgg
    13261 ccgcatccgt gacgactcga tcgcccagat ccgtgaggcg accgacatcg tcgccctggt
    13321 cggcgagtac gtgagcctcc gtcccgccgg cggcacccgc atgaagggcc tttgtccctt
    13381 ccatcaggag aagactccgt ccttcacggt cgaatccgtt aagggggtct ggcactgttt
    13441 cggctgccag gaaggcggcg acgcgatcga ttttctgcag aagcaggcca gcctgtcgtt
    13501 tgtggaggcg accgagcggc tggcccagcg agccgggatc gagctgcgct acgagggccg
    13561 ggcggtgggg gagcggggca gcctcgggcg taagagccgg ctggtggccg cccacgccga
    13621 ggcggtggcg ttctaccacc agctgctgct cagctcgccg gaggcgcggc cggcgcgggg
    13681 ctacctgtcg tcgcggggtt tcgaccgggc cgccgccgag cgcttcaagc tcggctgggc
    13741 gcccggggac cagtgggagg cgctggtgac ccatttgcgc ggcaagggct acggctcgca
    13801 ggagctgatc gacgcgggac tcgccaagcc gggcaaccgg ggcctgcggg atgccttcca
    13861 cgggcgggtg ctgttcccga tcttcgacgt ggccggcgac ccggtcgcct tcggtgcccg
    13921 catcctcgac ctcgacgacc agggtggctc agggcagggt ggcccagagc atggcggttc
    13981 gggtcgaggg gcggaccggg gaccgaaata cctgaacacc gccgagacgg tgatctggcg
    14041 caagggccag gtcctctatg ggctcaactg ggccaaggtc ccgatcgtgc aggcgagctt
    14101 cgcggtggtg gtcgagggct acaccgacgt gatcgcctgc caccgggctg gcgtgaccca
    14161 ggcggtggcc acctgcggga cggcgctgcg agaggatcac ttcaagttgc tcgcccgttt
    14221 caccgaaaaa atcgtgcttg cttttgacgc ggatacggct ggtggtaacg cggcaggacg
    14281 aggggtcaat gagttgcccc gtccagatca ggcaagtggc gggaccggct ttgccttcct
    14341 cggttcgtcg atgggtcagt tgatcgctga tcgcgtagcg gccctgacga cctacgtcct
    14401 tgtcatgcca gcgggacaag atccagctga tcttgttgta gaacgtggag gggccgcatt
    14461 tcgagcgctc atcgaccaag ctcaacctct ggtcggatgg tggcttgatc ggaagctcgc
    14521 tgactttgac ctgcgcaacc cggaaggcaa ggtgcgtgcc gcccgtgagc tggtgccgct
    14581 gctccgcagt attcccgatt cgctgcagcg cactgagtac gcccgctcgg tggtgcagcg
    14641 cctccacctg gacgaacggg agtacctggc gatggtggcc ggtcagcagg cgccctcccg
    14701 ggtccgggtg ggcgagccca aggagccgcc ccgtacccgc accccgcagc tgcgggtgga
    14761 atgcgaggcg ctcaagttcg ccctgcagca ttcagattgg accgccgaga cggcggtcag
    14821 ctgggaggct gactggttca ccgcgccctc caccaaggcg tcctttgccg ccctggtcaa
    14881 ggcgggcggg ccgggcgcgc cgcttgaggc ggtgctggag gcggccgcca atgagaccga
    14941 gcgccgcttc ctgcgcggcc tcgcggttga ggcattcgcg gcggaggaga accgggggta
    15001 cgctggcgag gtattccggc gactcgaaga gttccggcta acccgcgtaa tcgaccagct
    15061 caaggggacc ttgcaacgga tgaacccggt agagcggccg gacgagtaca ctcatctctt
    15121 cgaggaactg atcgccttcg aggcgcgacg gcgagccctg cgggagccgc ggcctgaagg
    15181 cgacggggac gccatccttc agcgccccat atagggacgc agcgccccac gtcccgcacg
    15241 acacgagagg tacttgacca gatggctatg gccaaggaag cccagatcga cgaggtgcgc
    15301 gatctcgtcg ccaagggcaa ggagcgcggc ttcctcacca acgaagaggt gatcgaggcg
    15361 ctggcgccgg tggacgtctc ggccgagcag atggacaaca tcctccagca tctgcaggac
    15421 gagaacatcg aggtcgtcga gatggtcgac gagctcgagg ccgaggagtt cgcgcgcgag
    15481 gcccgctccg cacgagacga cgagctcgcc ctcaaggccc ccaccaacga ccccgtccgc
    15541 atgtacctga aggagatcgg caaggtcccc ctcctcaccg cggagcagga ggtcatcctc
    15601 gccaaggcca tcgaggaggg cgaggccgcc accgccgagc tcgacaaggc cgccgatagc
    15661 ggcaagaagc tctccaccac ccgcctgcgg gagctgcaac gggtcgagcg gcagggccag
    15721 ctcggcaaga agaagctgat cgaggccaac ctgcggctgg tggtgtcgat cgccaagcgc
    15781 tacgtcgggc gcgggatgct gttcctggat ctgatccagg agggcaacct cgggctgatc
    15841 cgagcggtcg agaagttcga ctacaccaag ggctacaagt tctccaccta cgcgacctgg
    15901 tggatccgcc aggcgatcac ccgcgcgatc gccgaccagg cccgcaccat ccgcatcccg
    15961 gtccacatgg tggagaccat caacaagctg atccgcatcc agcgccagct cctccaggac
    16021 ctcggccgcg agccgacccc cgaggagatc ggccgcgaga tggagttctc cccggagaag
    16081 gtccgcgaga t
//
LOCUS       DASWDC010000058         4335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_846451, whole genome shotgun sequence.
ACCESSION   DASWDC010000058 DASWDC010000000
VERSION     DASWDC010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846451"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..805
                     /locus_tag="VF995_01920"
     CDS             38..805
                     /locus_tag="VF995_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9342358.1"
                     /translation="MRIDGAVVLLTGASSGIGAATAPLLAERGARLLLAGRDRDALAA
                     AAERCGGQVLAADLADPGATERLAAEALACHGRVDLLINNAGAGWAGPLAEMALDRAA
                     RLVALNLLAPMALARLLLPGMLERRHGHLVFVTSIAGVTGVGGEAVYAAAKGGLATFA
                     ASLREELSGRGVGISLVVPGVVATPFFDRRGTPYARTWPRPIAPERVAAALLRAVESD
                     RAEVFAPAWMRFPARLAGSAPALYRTLAIRFGATRPS"
     gene            complement(802..1806)
                     /gene="hpnH"
                     /locus_tag="VF995_01925"
     CDS             complement(802..1806)
                     /gene="hpnH"
                     /locus_tag="VF995_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEX9342359.1"
                     /translation="MGVPLRQGMRMGAYLLKQKLAGREKFPLLVELEPLFACNLACAG
                     CGKIQYPAHILKQRMPVETAVAAIEECGAPMVSIAGGEPLIHPEIHQIAAELVKRKKF
                     VYLCTNALLMERKLDRFIPSPYFAWAVHLDGLRERHDESVCQEGVFDKAVAAVREAQR
                     RGFRVTTNTTFFNTDTPQTVREVLDYLNDDLKVDQMMISPAYAYWKAPDQEHFLGVEE
                     TRELFRKAFADGGRKRWRLNHSPLFLDFLEGKADFQCTAWGIPSYSVFGWQRPCYLMS
                     DGYVPTYKELVETTDWSKYGRGRDQRCANCMAHCGYEPSAVLATMGSLKQSLRAISGS
                     "
     gene            complement(1806..3332)
                     /locus_tag="VF995_01930"
     CDS             complement(1806..3332)
                     /locus_tag="VF995_01930"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX9342360.1"
                     /translation="MQQPDQPQLAVAVALAVEGYAVSRALPGARVIRTGMGPERAEQA
                     AVGLFSSSDPSPLAIAGFCAGLDPALEPGDVVVASEVRGVRGTVPCPSAGLLVAALAR
                     RGIRARLGPLESVNHMVTGDERARIHADGPIAADMESAWLAAGAGAAAGRPLAVLRVV
                     TDSPGRELVRPGIVGNGVRAYRALRRAAPALADWAAACSPEPRRVLLAGPRSFCAGVE
                     RAIEVVERALELHGAPVYVRKQIVHNVHVVRRLEELGAVFVDELDEVPEGAMVVFSAH
                     GVSPAVRHEGAARELRVIDATCPLVSKVHAEARRFADYGETVVLIGHAGHEEVEGTMG
                     EIPEAVRLVENVSDVDRLEVDDPQRIAYLTQTTLAVDEAGAIVEALRRRFPALRGPSS
                     DDICYATTNRQDGVREVAQAADVVLVVGSENSSNSKRLVEVAEREGCPAHLLDDHTQL
                     DPAWLAGARTVGLTAGASAPESLVTEVMDALRGLGPVEGSEHPVVIESVRFTLPKEVN
                     "
     gene            complement(3332..>4335)
                     /gene="shc"
                     /locus_tag="VF995_01935"
     CDS             complement(3332..>4335)
                     /gene="shc"
                     /locus_tag="VF995_01935"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX9342361.1"
                     /translation="TIVESDIRRLEACQSPVWDTALALIGLGDAGLPPDHDAMVRGTR
                     WMLGEEVGVKGDWAVRRPELDPGGWAFEFENDVYPDIDDTAEVIMALRRVSSETPGLE
                     AAVRRGVEWTIGMECTDGAWGAFDADNTQELCRKLPFCDFGEVIDPPSADVTAHVVEM
                     LAREGMATHPAVERGVEWLLGEQEADGSWFGRWGANYLYGTGAAVPALVAAGMKPSHP
                     AIVRAVRWLESVQQPGGGWGEDLRSYDDPGAWRARGEPTASQTAWALLALLAAGRHDS
                     EAVRRGTAWLAETQRPDGSWDEPQFTGTGFPGDFSINYHLYRIVFPVMALGRYVRGR"
BASE COUNT          564 a         1595 c         1574 g          602 t
ORIGIN      
        1 tgcagcgctt cgctgagacc gtggaagggc gggcgggttg aggatcgacg gtgccgtggt
       61 gctgctgacc ggggcgtcga gcggcatcgg cgcggccacg gcacccctgc tggccgagcg
      121 gggcgcgcgg ctgctgctgg cgggccggga ccgcgacgcg ctcgccgctg ccgccgagcg
      181 ctgcggcggc caggtcctcg ccgccgacct cgctgacccg ggggcaaccg aacggctcgc
      241 cgccgaggcg ctggcctgcc atggccgggt cgacctgctc atcaacaacg ccggggccgg
      301 ctgggccggg ccgctggccg agatggcgct cgaccgcgcc gcccggctcg tggccctgaa
      361 cctgctcgcc ccgatggcac ttgcccggct gctgttgcct ggcatgctgg aacgccgcca
      421 cggccacctg gtcttcgtca cctcgatcgc cggtgtcacc ggtgtcggtg gcgaggcggt
      481 ctacgccgcc gccaaaggcg gccttgccac cttcgccgcc agcctccgcg aggaactctc
      541 cggccggggc gtcggcatct ccctggtcgt gccaggggtg gtcgcgacgc ccttcttcga
      601 ccgccgcggc accccctacg ctcgcacctg gccccgcccg atcgctcccg agcgggtcgc
      661 cgctgccttg ctccgcgcgg tcgagtcgga ccgcgcggag gtattcgctc ccgcctggat
      721 gcgcttcccg gcccgcctgg ccggctcggc acccgccctc taccggaccc ttgccatccg
      781 cttcggcgcc acccggccca gctagctgcc actgatcgcc cgcagggact gcttgaggct
      841 gcccatggtg gcgaggacgg cgctgggttc gtagccgcag tgggccatgc agttggcgca
      901 gcgctggtcg cggccacggc cgtacttgga ccagtcggtg gtctcgacca gctccttgta
      961 ggtcgggacg tagccgtcgg acatcaggta gcagggacgt tgccagccga agacggagta
     1021 gctggggatg ccccaggcgg tgcactggaa gtcggccttg ccctccagga aatccaggaa
     1081 caacggcgag tggttgaggc gccagcgctt gcggcccccg tcggcgaacg ccttgcggaa
     1141 cagctcgcgg gtctcctcca cgccgaggaa gtgctcctgg tcgggcgcct tccagtaggc
     1201 gtaggcgggg gagatcatca tctggtcgac cttcaggtcg tcgttcaggt agtcgagcac
     1261 ctcccgcacg gtctgcgggg tgtcggtgtt gaagaaggtg gtgttggtgg tcacccggaa
     1321 gccgcggcgc tgggcttccc ggacggcggc gaccgccttg tcgaacacgc cctcctggca
     1381 gacggactcg tcgtggcgct cgcgcaggcc gtcgaggtgg accgcccagg cgaagtacgg
     1441 cgacgggatg aaccgatcga gcttcctctc catcagcagg gcgttggtgc agaggtagac
     1501 gaacttcttg cgcttgacca gctcggcggc gatctggtgg atctcggggt ggatcagcgg
     1561 ctcgccgccg gcgatcgaga ccatcggcgc gccgcactcc tcgatcgccg cgaccgcggt
     1621 ctcgaccggc atgcgctgct tgaggatgtg cgccgggtac tggatcttgc cgcagcccgc
     1681 gcaggcgagg ttgcaggcga acagcggctc cagctcgacc agcaggggga acttctcccg
     1741 gccggccagt ttctgcttga gcaggtaggc ccccatccgc atgccctgcc gcagtggcac
     1801 ccccatcaat tgacctcctt cgggagcgtg aaacggaccg attcgatgac caccgggtgc
     1861 tcggagccct ccaccgggcc gagcccccgg agagcgtcca tgacctcggt gaccagcgac
     1921 tccggggcgg aggcgccggc ggtgagcccg accgtgcgcg ccccggcgag ccatgccggg
     1981 tcgagctggg tgtggtcgtc gagcaggtgg gccgggcagc cctcgcgctc ggcgacctcg
     2041 accaggcgtt tggagttgga ggagttctcc gagccgacca ccagcaccac gtcggcggcc
     2101 tgggcgactt cgcggacgcc gtcttggcgg ttggtggtgg cgtagcagat gtcgtcggag
     2161 gaggggccgc gcagggcggg gaagcggcgc cgcagcgcct cgacgatcgc gccggcctcg
     2221 tcgaccgcca gggtggtctg ggtgaggtag gcgatccgct gggggtcgtc cacctccagc
     2281 cggtccacgt cggagacgtt ctcgaccagg cggaccgcct cggggatctc gcccatggtc
     2341 ccctcgacct cctcatggcc ggcgtggccg atcagcacca ccgtttcgcc gtagtcggcg
     2401 aagcggcgcg cctcggcgtg gaccttggag accagcgggc aggtggcgtc gatcacccgc
     2461 agttcgcggg cggcgccctc atgccgcacc gccggggaga ccccgtgggc ggagaacacc
     2521 accatcgccc cctcgggcac ctcgtcgagc tcgtcgacga agaccgcgcc cagctcctcc
     2581 agccgccgca ccacgtggac gttgtggacg atctgcttgc ggacatagac cggggcgccg
     2641 tgcagctcca gggcgcgctc gaccacctcg atggcgcgct cgaccccggc gcagaacgag
     2701 cgcggcccgg ccagcagcac ccgccgcggc tccggcgagc aggccgccgc ccagtccgcc
     2761 agcgccggcg cggcgcgccg caacgcgcgg taggcgcgca cgccgttgcc gacgatcccg
     2821 gggcggacca gctcgcgccc ggggctgtcg gtcaccaccc gcagcaccgc cagcggccgc
     2881 ccggcggcgg cgccggcacc ggcggccagc caggccgact ccatgtcggc ggcgatcggc
     2941 ccgtcggcgt ggatgcgggc gcgctcgtcg ccggtgacca tgtggttgac cgactccagc
     3001 ggcccgagcc gggcgcggat ccctcgccga gccagggcgg ccaccagcag ccccgccgag
     3061 gggcagggga cggttccccg caccccgcgg acctcgctgg ccaccaccac gtcgccgggc
     3121 tccagcgccg ggtcgagccc ggcgcagaag ccggcgatcg ccagcggcga cgggtcggag
     3181 gagctgaaca ggccgacggc ggcctgctca gcccgttccg ggcccatgcc ggtccggatc
     3241 acgcgggcgc cggggagcgc gcggctgacc gcgtacccct cgaccgcgag cgcgacggcg
     3301 acagccagct gcggctggtc gggttgctgc attatcgccc cctcacgtat cggccaagtg
     3361 ccatgaccgg gaagacgatc cggtacaggt ggtagttgat ggagaagtcg ccggggaacc
     3421 cggtgccggt gaactgcggc tcgtcccagg agccgtcggg gcgctgcgtc tcggcgagcc
     3481 aggccgtccc ccgccgcacc gcttcgctgt cgtggcggcc ggcggcgagc agcgccagca
     3541 gcgcccaggc ggtctgcgat gcggtcggct cgccccgcgc ccgccacgcc cccgggtcgt
     3601 cgtaggagcg caggtcctcg ccccagccgc cgccgggctg ctgcacgctt tccagccagc
     3661 ggaccgcccg gacgatcgcc ggatgggacg gcttcatccc agcggcgacc agcgcgggga
     3721 cggccgcgcc ggtgccgtag aggtagttgg cgccccagcg gccgaaccag gagccgtcgg
     3781 cctcctgctc gccgagcagc cactcgaccc cgcgctccac cgccggatgg gtcgccatcc
     3841 cctcgcgggc gagcatctcg acgacgtggg cggtgacgtc ggcgctcggt gggtcgatca
     3901 cctcgccgaa gtcgcagaac ggcagcttgc ggcagagctc ctgcgtgttg tcggcgtcga
     3961 aggcgcccca ggccccgtcc gtgcactcca tcccgatcgt ccactcgacg ccccgcctga
     4021 ccgccgcctc gagcccgggc gtctcggagg acacgcggcg gagggccatg atcacctcgg
     4081 ccgtgtcgtc gatgtccgga tagacgtcgt tctcgaactc gaacgcccag ccgcccgggt
     4141 cgagctccgg ccggcggacg gcccagtcgc ccttgacgcc gacctcctcg ccgagcatcc
     4201 agcgggtgcc gcgcaccatt gcgtcgtggt cggggggaag accggcgtca ccgaggccga
     4261 tcagcgcgag cgcggtgtcc cagaccgggg actggcaggc ctcgagccgg cggatgtcgc
     4321 tctcgacgat cgtga
//
LOCUS       DASWDC010000059         9762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1598542, whole genome shotgun sequence.
ACCESSION   DASWDC010000059 DASWDC010000000
VERSION     DASWDC010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598542"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VF995_01940"
     CDS             <1..1202
                     /locus_tag="VF995_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342362.1"
                     /translation="KRVTHEDFWALRDVDLEIGTGQTVGLIGANGSGKSTLLKLLAGI
                     LEPTRGRVRVLGRIASLLELGAGFNGELSGRDNIYLNASILGLSRREIERNFDEIVAF
                     SGLEQFIDNQIKHYSSGQYVRLGFAIAVHVNPDILLVDEVLAVGDEAFQKKCLDKIAE
                     FQARGCTILFVTHALDLIPRLCTRAVVLDHGRVLHDGDPLEATDKLRGLLGTAADQTG
                     GAGTAASSLRISGARLYDPASAAQKGEFRAGEPMGIEIDVTAGAEPPAAASVRLAITG
                     PVDVPMWVMDTEPQRFEAGTTSTIRFTVKELPPLQGIYAFAVALRDPARGVAYDARRF
                     GEAFRAVGADDRGLIDVAWTADRGKFEEERNRAKPTTSRGTAGRGPSSSTSTSSRGTG
                     SGRRPRR"
     gene            1216..2616
                     /locus_tag="VF995_01945"
     CDS             1216..2616
                     /locus_tag="VF995_01945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9342363.1"
                     /translation="MTVITVNYKRADLLPPCLEALAAQDLPAGSVRVWVVDNASGDGS
                     VELLRRDFPWVRVFANQRNDGFAGGNNLALREVDTPYVALVNNDARPAPDWLRRLVEP
                     LEAATPGEQLGATTSKLVFLPRFLPLRVSTPGFTPGTLDTRELGVRIYRVLVDGHDVT
                     DLVLWDRLAYGPEGEGEGRFRWTHPSGELLIPVDPDGSGPAASAAPLRITLKLAAETA
                     KPVELTWGGAAGGSGNGGSGNGGGAATVKAGTEPAEADFTVAPGAALADVLNNAGSML
                     WSDGYGADRGYQELDRGQFERPEEVFTFCGAAVCFRREALADAGLFDDDFFLYYEDTD
                     LAWRLRSRGWAIRYQPDSVVRHIHSASSQEWSPLFVFHVDRNRMLMLTKNARAGLALR
                     EVLRYPLTTASLLARGFAQARHTHRRPPLRPTLLRLRVMASYLRLLPRMLARRRQIAA
                     RAAVSRRELANWFVPR"
     gene            2621..3799
                     /locus_tag="VF995_01950"
     CDS             2621..3799
                     /locus_tag="VF995_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9342364.1"
                     /translation="MTVGRSLRVGLDATPLLGPRTGVGHYVTCLVRALVELGPEESEL
                     EEVVLVPFSWRGTRDLPAVPPWDDPSGSGSGSGSRVDSRVRYGSRRVPARLLREGWAR
                     GNWPPVEWFAGAVDVFHATNFVAPPARRAGLVVTVHDLSYLRYPEMVSAASARYRELV
                     PRGIARGAVVCTPTAAVAAELAEAYRVPAERLVVTPLGVGPEWLQAQAPDSDWLVERG
                     LPEHYLLFVGSREPRKNLATLLAAYRQLPPGPDRPALVLAGPQGWGEALDLRGVITPG
                     YLPDEDLLRVVAGAAALVFPSWYEGFGFPPLQALAAGTPVVAADIPALREVTGGQAEL
                     VDPADADALAAALAKVLAGGDGGPAARAARAAHAAAFTWSATARATLGAYRLAAARRP
                     "
     gene            3843..5075
                     /locus_tag="VF995_01955"
     CDS             3843..5075
                     /locus_tag="VF995_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9342365.1"
                     /translation="MTAQPPWPQGRWLQLPPEPPRPPRVGVVAEQLFGPVPGGVGRYV
                     QALVRHLPAVAAAAEGGGAVRWITARHSSAELAEAGLPAERPAGVEAEATDGLTVPLS
                     LPGRVLTRTWVEARQPRLPLWLLRQLDLVHATSAAVPPARGKPLVATVHDVAFRRFPD
                     AYPASGRHFHERAARIVADEAARVLVPSAATARDLTEFYGVEAERITVTPLGAEPPPA
                     PDPGKARGLLDRLGVRGPFLLAVGTLEPRKNLLRLVAAFAEAAAELPEHHLVVAGPAG
                     WGASLRPEAVPPRVALAGPVDEATLHGLYDAADLLAYPSTYEGFGLPVVEAMLHGTPV
                     LTSDRSSLPEVAGDAALLVDPTDEHAIAKGLVELTADRALRDRLIAAGRARAARFTWE
                     ATAAATWAAYQEVLRAHG"
     gene            5062..6288
                     /locus_tag="VF995_01960"
     CDS             5062..6288
                     /locus_tag="VF995_01960"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9342366.1"
                     /translation="MPTDREEAVAAASLRVAVDATAIPARLTGAGIYVARMLAALAGR
                     IQRQRDSGSGEGGEGEGGEGEGGPEAALDLEVFAAPGSLGDLAVPGLQLHAVRAAGRG
                     RPARIAWSQLAAATAARRAGADLLHGVHYELPLQASMPQVVTVHDLTMITHPEWHEAA
                     KVRWFRWAMRRAVARAQRVLCVSATTARDLTERLGVDPARVDVTPLGTDLRRASDGEV
                     AALRARFGLGEREPYLLGLGTLEPRKDLPTLVRAFALLARDLPHRLVLAGLAGWGAGE
                     LRAVVAQSGVADRVLFPGYVPEPDKAALFSGADAFVYPSRYEGFGLPVLEAMACGVPT
                     ITTTGGSLPEVAGDGALLVDPGDPDVLAVAVAKLLGDPDEAAALARRGLARAAAQTWD
                     RCAALTAAAYRRAAAR"
     gene            complement(6291..6719)
                     /locus_tag="VF995_01965"
     CDS             complement(6291..6719)
                     /locus_tag="VF995_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9342367.1"
                     /translation="MIVVDTGALYAVFDRAEPRHERFATFLTRCTEGLVTTPLVLAEL
                     DHLLGTRLGEAVRSRVMLRLSAGAITLSPFDSPAFARAAQVADQHSDMRLGLTDASLM
                     VAAARHHTTRLLTADHKHFRAVRPLDGGDDAYTLLPADAS"
     gene            complement(6716..6964)
                     /locus_tag="VF995_01970"
     CDS             complement(6716..6964)
                     /locus_tag="VF995_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX9342368.1"
                     /translation="MVRTTIYLPERLKERIEAYAARHGSTEAAVIREAVETLLGNEPD
                     DRSWVQELAGIGRSEAGGPVADRVDEALAETGFGTRHQ"
     gene            7071..8189
                     /locus_tag="VF995_01975"
     CDS             7071..8189
                     /locus_tag="VF995_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX9342369.1"
                     /translation="MQAVILAGGFGTRLRPLTSTRPKPLIPFANEPFLLHMLRQLAAG
                     GFSEVVLSTMYLPEAFDEVIPAARKFGVEVTLSSEDTPMGTSGAVKRLADLLEGTFLV
                     LNGDILVDLDFERLVQIHRDRGAAATLALVRVPDPSAFGLVPIDAEGRVQAFLEKPGP
                     DEEAWVTDLINAGVYVLEPSVLDHVPAGKPSSFERNLFPSLLASGALVVGTELHGYWR
                     DLGTPIAYLAAQADLLEGRLNLPISATEHGRYQWLAEGAEIADGAVLRGPVLIGPGAR
                     VEAEGRVFGPSVLGPGAVVAGGARCERSVLLAGARIEQGGKVFDSIIGAGVVVGSGAV
                     VGDGAVLGDNVVIDEGNVLAGGLRIAPGVHLPPGAIRV"
     gene            complement(8233..8466)
                     /locus_tag="VF995_01980"
     CDS             complement(8233..8466)
                     /locus_tag="VF995_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342370.1"
                     /translation="MSMTRTTVCLTQDTKRRLRLAAQRRQCSEAELVREAIDALLASE
                     PERPRPNLPLFTGVDHTVADRVDDIPAAQVPPR"
     gene            8858..>9762
                     /locus_tag="VF995_01985"
     CDS             8858..>9762
                     /locus_tag="VF995_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342371.1"
                     /translation="MTRRGPLFAVVTVVVILAALGVPLVAKALVGATAQTAAQPDGRV
                     SAIAVSGSTIYLGGQFTHVKDLGGTSQARAHLAAVDASTGELLPWAPSTNLPVDALAV
                     SAAGTVFAGGEFTTVNGSARARLAALDGASGALGTWNPGADNTVRALAVSAVNDRLYA
                     GGDFKTIAGTARVRLTAFSLGTGALDATWKPKATGTVNSLALAPGEGRVYAGGAFKSV
                     NSNTAAAFAAAINPTSGALDTTFTASFGGQAGFKLYSLAADADGLYAAGGGVGGHLVW
                     WNADGSLHQPIYLTDGGVQAVTALG"
BASE COUNT         1188 a         3692 c         3536 g         1346 t
ORIGIN      
        1 ccaagcgggt cacccatgag gacttctggg cgctgcgcga cgtcgacctg gagatcggca
       61 ccggccagac ggtcgggctg atcggcgcca acgggtccgg caagtcgacc ctgctgaagc
      121 tgctcgccgg gatcctggag ccgacccgcg gcagggtcag ggtcttgggg cggatcgcct
      181 cgctgctgga gcttggcgcg ggcttcaacg gggagctgtc cggccgcgac aacatctacc
      241 tcaacgcctc gatcctgggg ctgtcgcgcc gcgagatcga gcgcaacttc gacgagatcg
      301 tggcgttctc gggcctggag cagttcatcg acaaccagat caagcactac tcctccggcc
      361 agtacgtgcg gctcggcttc gccatcgctg tccacgtcaa cccggacatc ctgctggtcg
      421 acgaggtgct ggcggtcggc gacgaggcgt tccagaagaa atgcctcgac aagatcgcgg
      481 agttccaggc gcgcggttgc accatcctgt tcgtcaccca cgccctcgac ctgatccccc
      541 ggctctgcac ccgggcggtg gtgctcgacc acggccgggt gctgcacgac ggcgacccgc
      601 tggaggcgac cgacaagctc cgcggcctgc tcggcaccgc cgccgaccag actggcggcg
      661 ccggcacggc cgccagcagc ctgcggatct ccggggcgcg cctgtacgac ccggcgtcgg
      721 cggcgcagaa gggcgagttc cgcgcgggcg agccgatggg catcgagatc gacgtgaccg
      781 ccggggcgga gccgcccgcg gcggcgtcgg tgcggctggc gatcaccggg ccggtggacg
      841 tgccgatgtg ggtgatggac accgagccgc agcggttcga ggcgggaaca acctcgacca
      901 tccgcttcac ggtgaaggag cttccaccgc tgcagggcat ctacgcgttc gcggtcgccc
      961 tccgcgaccc ggcccgcggc gtggcgtacg atgcccgccg cttcggcgag gcgttccggg
     1021 cggtcggcgc cgacgaccgc ggcctcatcg acgtggcctg gaccgccgac cgcggcaagt
     1081 tcgaagagga gaggaaccgt gccaagccca ccaccagccg gggaaccgcc ggccgggggc
     1141 cctccagctc cacctccacc tccagccggg gaaccggcag cggccgccgg cccaggcggt
     1201 gacgctcgcg cgatcgtgac cgtcatcacg gtcaactaca agcgcgccga cctgctgccc
     1261 ccctgcctgg aggcgctcgc cgcccaggac ctgcccgccg ggtcggtgcg ggtctgggtg
     1321 gtcgacaacg cctccggcga cggctcggtg gagctgctgc ggcgcgactt cccctgggtc
     1381 cgggtgttcg ccaaccagcg caacgacggc ttcgccggcg gcaacaacct cgccctgcgc
     1441 gaggtcgaca cgccctacgt ggcgctggtc aacaacgacg cccgcccggc gcccgactgg
     1501 ctgcggcggc tggtcgagcc gctggaggcg gcgacgcccg gcgagcagct cggcgccacc
     1561 acctccaagc tggtgttctt gccgcggttc ctgccgctgc gggtctccac gcccgggttc
     1621 accccaggca cgctcgacac ccgcgagctg ggcgtgcgga tctaccgggt gctggtcgac
     1681 ggccacgacg tgaccgacct ggtgctgtgg gaccgcctcg cctacgggcc ggagggcgaa
     1741 ggggagggcc gcttccgctg gacccacccc tccggtgagc tgctcatccc cgtcgacccc
     1801 gacggcagcg gccccgcggc cagcgccgcg ccgctgcgga tcaccctgaa gctcgccgcc
     1861 gagaccgcca agccggtcga gttgacctgg ggaggggcgg cgggcggcag cggcaatggc
     1921 ggcagcggca atggcggcgg cgccgcgacc gtcaaggccg gcaccgagcc ggccgaggcc
     1981 gacttcaccg tcgcgccggg ggcggcgctg gccgacgtgc tcaacaacgc cggcagcatg
     2041 ctgtggagcg acggctacgg cgccgaccgc ggctaccagg aactggaccg gggccagttc
     2101 gagcggcccg aggaggtgtt caccttctgc ggcgccgcgg tctgcttccg ccgcgaggcg
     2161 ctcgccgacg ccggcctgtt cgacgacgac ttcttcctct actacgagga caccgacctg
     2221 gcctggcggc tgcgctcccg cggctgggcg atccgctacc agcccgactc ggtggtccgc
     2281 cacatccatt cggcgtcgtc acaggagtgg tcgccgctgt tcgtcttcca cgtcgaccgc
     2341 aaccgcatgc tgatgctcac caagaacgct cgcgccggcc tcgccctgcg ggaggtgctg
     2401 cgctacccgc tcaccaccgc gtcgctgctg gcccgcgggt tcgcgcaggc ccgccacacc
     2461 caccgccgcc cgcccctgcg cccgaccctg ctgcgcctgc gggtgatggc ctcctacctg
     2521 cgcctgctgc cccgcatgct ggcccgccgc cgccagatcg ccgcccgcgc cgctgtcagc
     2581 cgccgcgagc tggcgaactg gttcgtccca cggtgagccc gtgacggtcg ggaggtcgct
     2641 gcgggtcggg ctggacgcca cgccgctgct cggcccccgc accggcgtcg gtcactacgt
     2701 gacctgcctg gtccgggcgc tggtcgagct ggggccggag gagtcggagc tggaggaggt
     2761 cgtcctggtg ccgttctcct ggcgcgggac ccgcgacctg ccggccgtcc caccctggga
     2821 cgacccgtcc gggtccgggt ccggatccgg gtcccgcgtg gactcccggg tccgctacgg
     2881 atcccggcgg gtgcccgcgc ggctgctgcg ggaggggtgg gcgcggggca actggccgcc
     2941 ggtggagtgg ttcgcgggcg cggtggacgt cttccacgcg accaacttcg tggcgccccc
     3001 ggcccggcgc gcggggctgg tggtgaccgt gcacgacctc agctacctcc gctacccgga
     3061 gatggtcagc gccgcctcgg cgcgctaccg cgagctggtc ccccggggca tcgcgcgcgg
     3121 cgcggtggtc tgcaccccga ccgccgccgt cgccgccgag ctggccgagg cctaccgggt
     3181 gccggcggag cggctggtcg tcaccccgct cggggtcggc ccggaatggc tccaggcgca
     3241 ggcgcccgac tcggactggc tggtggagcg ggggctgccc gagcactacc tgctgttcgt
     3301 cggcagccgc gagccgcgca agaacctggc gaccctgctg gccgcctacc ggcagctccc
     3361 cccgggcccc gatcggcccg cgctggtgct ggccgggccg caagggtggg gcgaggcgct
     3421 cgacctacgc ggggtgatca cccctggcta cctgcccgac gaggatctgc tgcgggtcgt
     3481 ggccggcgct gccgcgctgg tgttcccgtc ctggtatgaa gggttcgggt tcccgccgct
     3541 gcaggcgctc gccgccggca ccccggtggt cgccgccgac atcccggcgc tgcgtgaggt
     3601 gaccggcggc caggccgagc tggtcgaccc ggcggacgcc gacgccctcg ccgccgcgct
     3661 cgccaaggtg ctggccggcg gcgacggcgg cccggcggcc cgcgccgcgc gggcagccca
     3721 cgccgccgcc ttcacctggt cggcgaccgc ccgcgccacc ctcggtgcct accggctcgc
     3781 ggcggcgcgg cgcccatgac aacgaaggcc atgccagccc tcgtcatcag gagggtggac
     3841 cagtgaccgc ccagccgccg tggccccagg ggaggtggct gcagctgccg ccggagccgc
     3901 cacggccccc gcgcgtcggg gtggtcgccg agcagctgtt cgggccggtg cccggcgggg
     3961 tcggccgcta cgtgcaggcg ctcgtccgcc acctgcccgc ggtcgccgcg gccgccgagg
     4021 gcgggggagc ggtccgctgg atcaccgccc gccactccag cgccgagctc gccgaggccg
     4081 gcctgcccgc cgagcgtccc gcgggcgtcg aggccgaggc caccgacggc ctcaccgtgc
     4141 cgctgtcgct gcccgggcgg gtgctgaccc ggacctgggt ggaggcgcgc cagccgcgcc
     4201 tgccgctgtg gctgctgcgc cagctcgacc tggtccacgc gaccagcgcg gcggtgccgc
     4261 ccgcccgcgg caagccgctg gtcgccaccg tccacgacgt cgccttccgg cgtttccccg
     4321 acgcctaccc ggcctccggg cgccacttcc acgagcgcgc cgcccggatc gtcgcggacg
     4381 aggcggcccg ggtgctggtg ccctcggccg cgaccgcccg cgacctcacc gagttctacg
     4441 gggtggaggc ggagcggatc accgtcaccc cgctcggcgc cgagccgccg cccgcgcccg
     4501 accccggcaa ggcccggggc ctgctggacc ggctgggggt gcgcgggccg ttcctgctgg
     4561 cggttgggac cctggaaccg cgcaagaatc tcctgcggct ggtcgcggcg ttcgcggagg
     4621 ccgccgccga gctgcccgag caccacctgg tggtcgccgg cccggcaggg tggggcgcgt
     4681 cgcttcggcc cgaggcggtc ccaccccggg tggcgctcgc cgggccggtc gacgaggcca
     4741 ccctccacgg cctctacgac gccgccgacc tgctcgccta cccctccacc tacgaggggt
     4801 tcggcctgcc ggtggtggag gcgatgctgc acggcacccc ggtgctgacc agcgaccgct
     4861 ccagcctccc cgaggtcgcc ggggacgccg ccctgctggt cgacccgacc gacgagcacg
     4921 ccatcgccaa gggcctggtc gagctgaccg ccgaccgcgc cctgcgggac cggctgatcg
     4981 ccgccgggcg cgcccgcgcg gcacgcttca cctgggaggc gaccgcggcg gcgacctggg
     5041 ccgcctacca ggaggtgctg cgtgcccacg gatagggagg aggccgtggc cgcggcctcg
     5101 ttgcgggtgg cggtggacgc caccgcgatc ccggcgcggc tgaccggcgc cgggatctac
     5161 gtggcccgga tgctggccgc gctcgccggc cgaatccagc gccagcggga cagcggcagc
     5221 ggcgagggcg gcgagggcga gggcggcgag ggcgagggcg gcccggaggc ggcactggac
     5281 ctggaggtgt tcgccgcgcc cgggtcgctg ggggacctgg ccgtacccgg gctgcaactc
     5341 cacgcggtgc gggccgccgg gcggggacgc cccgcgcgga tcgcctggag ccagctcgcc
     5401 gccgccactg ccgcccggcg cgccggggcg gacctgctgc acggcgtcca ctacgagctg
     5461 ccgctccagg cgagcatgcc gcaggtggtc accgtccacg acctcacgat gatcacccat
     5521 cccgagtggc acgaggccgc caaggtccgc tggttccgct gggcgatgcg ccgcgccgtc
     5581 gcccgcgccc agcgggtcct gtgcgtctcg gccaccaccg cccgcgacct caccgagcgg
     5641 ctcggcgtcg acccggcccg ggtcgacgtc accccgctcg gcaccgacct gcgccgcgcc
     5701 tccgacggcg aggtcgccgc gctgcgggcc cgcttcggcc tcggcgagcg ggagccgtac
     5761 ctgctcgggc tcggcaccct ggagccgcgc aaggacctgc cgacgctggt gcgggcgttc
     5821 gcgctgctcg cccgcgacct gccccaccgg ctggtgctcg ccgggctggc cggctggggc
     5881 gccggcgagc tgcgcgcggt ggtcgcccag agcggcgtcg ccgaccgggt cctgttcccc
     5941 ggctacgtcc ccgagcccga caaggccgcc ctgttcagcg gcgccgacgc cttcgtctac
     6001 ccgagccgct acgaaggctt cggcctcccc gtcctggagg cgatggcctg cggcgtcccc
     6061 accatcacca ccaccggcgg ctccctcccc gaggtcgccg gcgacggcgc cctcctggtc
     6121 gaccccggcg accccgacgt cctcgccgtc gccgtcgcca agctcctcgg cgaccccgac
     6181 gaggccgccg ccctcgcccg ccgcggcctc gcccgcgccg ccgcccagac ctgggaccgc
     6241 tgcgccgccc tcaccgccgc cgcctaccgc cgcgccgccg cacggtagcg tcaggacgcg
     6301 tcggcgggca gcagcgtgta ggcgtcgtcg ccgccgtcga gcggtcgcac ggcgcggaag
     6361 tgcttgtggt ccgcggtgag caggcgtgtg gtgtggtgtc gtgcggcggc aaccatcaga
     6421 gaggcatcgg tcaggccgag ccgcatgtcc gaatgctgat ccgcgacctg cgccgcccgg
     6481 gcgaaggctg gggaatcgaa ggggctgagg gtgatcgcac cggcggacag tcgcagcatg
     6541 acccggctcc gcaccgcctc cccgaggcga gtgcccagca gatggtcgag ctctgccagc
     6601 accagcggtg tggtgaccag cccttcggtg cagcgagtga ggaaggtcgc gaaccgctcg
     6661 tgtctcggtt ctgcccggtc gaagaccgcg tagagcgctc cggtgtccac cacgatcatt
     6721 ggtgacgggt cccgaagccg gtctcggcca aggcctcgtc aacccggtcg gcgaccggac
     6781 cgcccgcttc gctgcgcccg atgccagcca gttcctgcac ccaggagcga tcatcgggct
     6841 cattgccgag cagcgtctcg accgcctcgc ggatgaccgc cgcctcggtg ctgccgtggc
     6901 gggccgcgta ggcctcgatg cgttccttca gacgctcggg caggtagatc gtggtacgga
     6961 ccatgccgcc agtatgccat acctatatgg cgttctccag cgccgaacaa gccctacact
     7021 gcacccggat ctgtttggtg cccgtctgag caagcttcga ggtgaccgcc gtgcaagcag
     7081 tgattctggc tggggggttc gggacccgcc ttcgtccgct caccagtacc cggccgaagc
     7141 cgctgatccc gttcgccaac gagccgttcc tgctgcacat gctgcggcag ctcgcggccg
     7201 gcgggttcag cgaggtggtg ctgtccacca tgtacctgcc ggaggcgttc gacgaggtga
     7261 tcccggcggc gcgcaagttc ggcgtcgagg tgaccctgtc gtccgaggac acgccgatgg
     7321 gcacctccgg cgcggtcaag cggctcgccg acctgctgga gggcaccttc ctggtgctca
     7381 acggcgacat cctggtggac ctcgacttcg agcggctggt ccagatccac cgcgaccgcg
     7441 gcgccgccgc caccctcgcg ctggtccggg tcccggaccc gagcgcgttc gggctggtcc
     7501 ccatcgacgc cgaagggcgc gtccaggcgt tcctggaaaa gcccggcccc gacgaggagg
     7561 cctgggtcac cgacctgatc aacgccggcg tctacgtgct ggagccgtcc gtgctcgacc
     7621 acgtcccggc cgggaagccg tcctcgttcg agcgcaacct gttcccctcg ctgctggcct
     7681 ccggcgcgct ggtggtcggc accgaactgc acggctactg gcgcgatctc ggcaccccga
     7741 tcgcctacct cgccgcccag gccgacctgc tggaggggcg gctgaacctg ccgatcagcg
     7801 ccaccgagca cggccgctac cagtggctcg ccgaaggcgc cgagatcgcc gacggcgccg
     7861 tcctgcgcgg cccggtgctg atcggcccgg gcgccagggt ggaggccgaa ggccgcgtct
     7921 tcggcccgtc ggtgctcggc cccggcgcgg tggtcgccgg gggggcccgc tgcgaacgct
     7981 cggtgctgct ggccggcgcc cgcatcgagc agggcggcaa ggtcttcgac tcgatcatcg
     8041 gcgcgggcgt cgtcgtcggc tccggcgcgg tcgtcggcga cggcgccgtc ctcggcgaca
     8101 acgtcgtgat cgacgagggc aacgtcctcg ccggcggcct ccggatcgcc cccggcgtcc
     8161 acctcccccc gggcgcgatc cgggtctagg gagcgtcggc cgccaggatg gtgaacgcgg
     8221 gggaccgggt ggtcagcggg gcggcacttg ggcggctggt atgtcatcca ccctgtcggc
     8281 aacggtgtga tccacaccgg tgaacagcgg caggttcggc cggggtcgct caggctcact
     8341 ggcgagcagc gcatcgattg cctcgcggac gagttccgcc tcgctgcact ggcggcgctg
     8401 ggctgccagg cgcagccgac gcttggtgtc ctgggtgagg cagaccgtcg tcctcgtcat
     8461 gctcatggca aatacgctac caggtatggc ttcgtgcaag ggacttgtct ggtatgggaa
     8521 gatggattag tgcccggccg ggccctggag tggtctcgca gcctcctctg gtagcccggc
     8581 cgggcctttc gtatgcccaa tctgggcatg cttgtgtggt ccggggaggg tgctaccagg
     8641 ctaacttctc tatagccgcg ttgtgtagaa gcgtcgaggc ggcttcgtcc catttcgttc
     8701 atggatcctg ccgaggttcc ctcggtaggc gctgagcggc ccgagtggcg tagcgcgggt
     8761 cgcgtgaatg tgcccgacaa ttcaggcatc tggtgctagg ttcggacaga gatgtctgaa
     8821 taactcctct tcactgctgt cgagggagga gttggggatg acccgtcgcg gaccgctgtt
     8881 cgctgttgtg actgttgtgg tgattcttgc cgcgctgggg gtgccgctgg tggcgaaggc
     8941 gctggtcggc gcgacggcac agacagcggc gcagccggac ggccgcgtct cggcgatcgc
     9001 ggtcagcggc tccacgatct acctcggcgg gcagttcacc cacgtcaagg acctcggcgg
     9061 gacctcgcag gcacgcgccc acctggccgc ggtcgacgcc tccaccggcg agctgctgcc
     9121 gtgggcgccg agcaccaacc tcccggttga cgcgctggcg gtgtcggcgg cggggaccgt
     9181 gttcgccggc ggggagttca ccaccgtcaa cggatcggcc cgcgcgcgcc tggccgcgct
     9241 cgacggggcc agcggcgcgc tcggcacctg gaacccgggc gctgacaaca ctgtccgggc
     9301 cctggcggtc tcggcggtca acgaccggct gtacgcgggc ggcgacttca agaccatcgc
     9361 cggcaccgcg cgggtgcgcc tcaccgcctt ctcgctcggc accggcgcgc tcgacgccac
     9421 ctggaagccg aaggccaccg gcacggtcaa cagcctggcg ctcgcgcccg gcgagggccg
     9481 cgtctacgcc ggcggcgcct tcaagtcggt caacagcaac accgccgccg cgttcgccgc
     9541 cgccatcaac cccaccagcg gcgcgctcga cacgaccttc accgccagct tcggcggcca
     9601 ggccggcttc aagctctaca gcctcgccgc tgacgccgac gggctctacg cggccggcgg
     9661 cggcgtcggc ggccacctgg tgtggtggaa cgccgacggg tcgctgcacc agccgatcta
     9721 cctgaccgac ggcggtgtcc aggccgtcac cgccctcggc ga
//
LOCUS       DASWDC010000060         5336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_423843, whole genome shotgun sequence.
ACCESSION   DASWDC010000060 DASWDC010000000
VERSION     DASWDC010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423843"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VF995_01990"
     CDS             complement(<1..1298)
                     /locus_tag="VF995_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9342372.1"
                     /translation="MAWANEEIASVFREFAELLELRGETGFRLRAYDRAARALSGYDR
                     DLASLSQAELAAIPGVGKAIAAKAREYLEHGQVRALQELRAEVPEGLRVLRRVPGVGP
                     RKARLIHERLGVATIEDLTAAVAAGKIAGLPGMGARTEQNLRRALERLADGTHGQGIP
                     LADALPLAEWLRDQLARVAGIAIDEVEVAGSLRRMKAIVHDIDLLAAGPDPGAIARAF
                     AALDGVAEVAALGDTKVSILTRQGQQVDLRVVRPELWGAALQYFTGSKEHNVKVRELA
                     VKQGYKLSEYGLQTRGGELVVARDEAVVYQRLGLAWVPPALREDRGEVEAARDGALPR
                     LVAPGDVGGDFHCHTDLSPDGTAPLEVMVDAARARGYRFLAITDHAERLSLGGASRAQ
                     LLAQRERLRALERDRGDIVLLHGAELNIGPDGSLDYDDDFL"
     gene            complement(1305..1880)
                     /locus_tag="VF995_01995"
     CDS             complement(1305..1880)
                     /locus_tag="VF995_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342373.1"
                     /translation="MAAFRRSEPELTIGRAGAAAPAAPDPRSRFAGIVWPIAAALAGA
                     AASTLVTAGAAVVAGRQADLALQPVELALRVGLLLAAPCVGALLAALVLPPGGGRLAI
                     AGVGVGVPFGPLLVLRLDRMRDFGLAPTLLTAAVLVLWGVISALLVAGWWARPARTAV
                     ERTHLPPLPGPDPRSTPGPDSGTGPGVDGAA"
     gene            complement(1847..2779)
                     /gene="argF"
                     /locus_tag="VF995_02000"
     CDS             complement(1847..2779)
                     /gene="argF"
                     /locus_tag="VF995_02000"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX9342374.1"
                     /translation="MTKRDFLSDDDLTPGELTALLDGADQYRDDRRPRATLAGRSVAL
                     IFEKPSTRTRVSFEVAVHELGGYPLPLVASDLQLGRGETIEDTAQVLSRYVHAIVLRT
                     FAQENLERLARASTVPVINALSDYLHPCQALADLQTIRLRKGHLEGLRLGYLGDGNNV
                     AHSLLLAGAKVGMHLAIATPAGYEPIPQIVRRASEIATETGGSVELTNDPATAAKGAD
                     VLYTDVWASMGQELEHGERALIFKPYQLSTDTVNAAAADVVVLHCLPAHRGEEITAEV
                     MDGPSAAVFDQAENRLHTQKALLSWLLSGDPNPS"
     gene            complement(2776..3198)
                     /locus_tag="VF995_02005"
     CDS             complement(2776..3198)
                     /locus_tag="VF995_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9342375.1"
                     /translation="MLVVEDDASVRGLLQAILEDEAFDVVLATDGEEGLRLARAVKPA
                     VVLLDVMMPGLGGPEVLTRLRRPDGSLPFGVLVLTGAAELVTALRGELGPDAILEKPF
                     DITTLASRVKALAAAGDRQHGTHQQHGNHEHENHGGIR"
     gene            complement(3437..4696)
                     /locus_tag="VF995_02010"
     CDS             complement(3437..4696)
                     /locus_tag="VF995_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX9342376.1"
                     /translation="MVDYTVAGVEGGVCAPRGFRAAGVDAGLVAGPGSDLGLVVSQQT
                     AATSGRFTSHRFAAAPVRWSRRQLADGSARAVLVHAGAANAATGARGDADAAALAEAC
                     ATAVGCAPAEVLLLGTGPIGRRLPLEAAAEGAEKAVTALGRRGSAELARALAASGGVA
                     REHAVAVAWSEGEVRIGGVAKASVPFAPSFASTLVLLTTDATVEPPVLDGILERALGR
                     SFERILVDQGPSMADSAILLANATAAAPVLTAGSEGAAAFEAALAYLCESLGEQLAMR
                     VPGSRKLIVVTVHGAASHADALAAARAVAASIALRAALASGLPAWPAVLDAIDRAAIP
                     LDPARVGVRFGPVTVVTGGEAAAHDERAAAAVAAKGQVDLAVDLGAGSAAATITTTDL
                     PPEALGRFEAEGAGTVARGPDGMSDLK"
     gene            complement(4983..>5336)
                     /locus_tag="VF995_02015"
     CDS             complement(4983..>5336)
                     /locus_tag="VF995_02015"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9342377.1"
                     /translation="AAELELTPLLEAAPATRPAMTPSPYPELSFDVAFLVPPGVAAAG
                     LQATLSEAGAELLARLALFDAYQGAPLPPGYRNLAYRVALQAPDRTLTDADAAAVRDR
                     MVALAAERHDATLRA"
BASE COUNT          659 a         1764 c         2227 g          686 t
ORIGIN      
        1 aggaagtcgt cgtcgtagtc gaggctgccg tcggggccga tgttcagctc ggcgccgtgg
       61 agcaggacga tgtcgccgcg gtcgcgttcc agggcgcgga gccgttcgcg ctgggcgagc
      121 agctgggcgc ggctggcacc gccgagggag agccgctcgg cgtggtcggt gatggccagg
      181 aagcggtagc cgcgggcgcg ggccgcgtcg accatgacct ccagcggcgc ggtgccgtcg
      241 ggtgagaggt cggtgtggca gtggaagtcg ccgccgacgt cgcccggggc gaccagccgt
      301 ggcagggcgc cgtcgcgggc cgcctccacc tcgccgcggt cctcccgcag cgccggcggc
      361 acccaggcga ggccgaggcg ctgatagacg accgcttcgt cgcgggcgac gaccagctcg
      421 ccgccgcggg tctggaggcc gtactcgctg agcttgtagc cctgtttgac ggccagctcg
      481 cggaccttga cgttgtgttc cttggagccg gtgaagtact gcagcgcggc gccccagagc
      541 tcggggcgga ccacgcgcag gtcgacctgc tggccttgcc gggtcaggat cgagactttg
      601 gtgtcgccga gggcggcgac ctcggcgacg ccgtccaggg cggcgaaggc acgggcgatg
      661 gcgccggggt cgggcccggc ggcgagcagg tcgatgtcgt ggacgatggc tttcatgcgg
      721 cgcagcgagc cggcgacctc gacctcgtcg atcgcgatgc cggcaacgcg ggcgagctgg
      781 tcgcggagcc actcggcgag cggcagcgcg tccgcgaggg ggatgccctg gccgtgggtg
      841 ccgtcggcga ggcgctccag ggcacggcgg aggttctgct cggtgcgggc gcccatgccg
      901 ggcaggccgg cgatcttgcc ggcggcgacc gcggcggtga ggtcctcgat ggttgcgacg
      961 ccgaggcgtt cgtggatgag gcgcgccttg cgggggccga cgccggggac gcggcgcagg
     1021 acccgcagcc cttcgggaac ttcggcgcgg agctcctgaa gggcgcggac ctggccgtgt
     1081 tcgaggtact cgcgcgcctt ggcggcgatc gccttgccga cgccggggat ggcggcgagc
     1141 tccgcttggg agaggctggc gaggtcgcgg tcgtagccgg agagggcacg ggcggcgcgg
     1201 tcgtaggcgc gcagccggaa gccggtctcg ccgcgcagct cgagcagctc ggcgaactcg
     1261 cggaagaccg aggcgatttc ctcgttggcc caggccatcc cgtgctacgc ggccccgtcg
     1321 acgccgggtc cggttcccga gtcggggcca ggggtggacc tcgggtcggg tccggggagc
     1381 ggtgggagat gggtgcgttc gacggcggtg cgggcggggc gggcccacca gccggcgacc
     1441 agcagagcgc tgatcacgcc ccacaggacc aggacggcgg cggtgagcag ggttggggcg
     1501 aggccgaagt cgcgcatgcg gtcgagccgc aggaccagca ggggtccgaa cggcacgccg
     1561 acgccgaccc cggcgatggc gaggcggccg ccgccggggg ggagcaccag cgcggcgagc
     1621 agcgccccga cgcagggtgc ggcgagcagc agcccgaccc gcagggccag ctcgaccggc
     1681 tgcagggcga ggtcggcctg gcgaccggcg accacggcgg ccccggcggt caccagagtg
     1741 ctggcggccg cgccggccag cgccgccgcg atcggccaga cgatcccggc gaagcgcgaa
     1801 cgcggatcgg gcgccgccgg ggccgcggcg cccgccctcc cgatggtcag ctcgggttcg
     1861 gatcgccgga aagcagccat gacagcaggg ccttctgggt gtggaggcgg ttttcggcct
     1921 ggtcgaagac ggcggcgctg gggccgtcca tgacctcggc ggtgatctcc tcgccgcggt
     1981 gggcgggcag gcagtgcagc accaccacgt cggcggcggc ggcgttgacc gtgtcggtgg
     2041 agagctggta gggcttgaag atcagggccc gctcgccgtg ttcgagctcc tggcccatgg
     2101 aggcccagac gtcggtgtag aggacgtcgg cgcctttggc ggcggtggct gggtcgttgg
     2161 tcagctcgac gctgccgccg gtctcagtgg cgatctcgga ggcgcggcgc acgatctggg
     2221 ggatcggctc gtagccggcg ggggtggcga tggccaggtg catgccgacc ttggcgccgg
     2281 cgagcagcag gctgtgagcg acgttgttgc cgtcgccgag gtagccgagg cgcaggccct
     2341 cgagatggcc tttgcgcagg cggatggtct gcaggtcggc gagcgcctgg caggggtgga
     2401 ggtagtcgga gagggcgttg atcaccggga cggtgctggc gcgggcgagg cgctcgaggt
     2461 tttcctgcgc gaaggtgcgc agcacgatgg cgtggacgta gcgggagagg acctgggcgg
     2521 tgtcctcgat cgtctcgccg cggccgagct ggaggtcgga ggcgaccagc gggagcgggt
     2581 agccgccgag ctcgtgcacg gcgacctcga aggagacgcg ggtgcgggtg gagggcttct
     2641 cgaagatcag ggcgacactg cggccggcga gggtggctcg cgggcgccgg tcgtcgcggt
     2701 actggtcggc gccgtcgagc agggcggtga gctcgccggg ggtgaggtcg tcgtcggaga
     2761 ggaaatcgcg cttggtcatc ggatgccccc gtggttctcg tgctcgtggt tgccgtgctg
     2821 ctggtgggtg ccgtgctgcc ggtcgccggc ggcggccagc gccttgacgc ggctcgccaa
     2881 ggtggtgatg tcgaacggct tctccaggat cgcgtcgggg ccgagttcgc cgcgcagcgc
     2941 ggtgaccagt tcggccgcgc cggtcagcac cagcacgccg aaggggaggc tgccgtccgg
     3001 tcggcgcagc cgggtgagga cctcggggcc gccgaggcct ggcatcatca cgtcgaggag
     3061 cacgacggcc ggcttgaccg cgcgggcgag ccgcagcccc tcctcgccgt cggtggcgag
     3121 gacgacgtcg aacgcctcgt cctcaaggat cgcctggagc agcccgcgaa cgctggcgtc
     3181 gtcctctacc accagcacgc gattcgaacc ccgggcgagg ttcccttctt gaggtggttg
     3241 cgtggccgcg gtggacggcg cgcacggcga agcgccgcac ggttgtgtcg gcgcttcatg
     3301 cgacgccgga gactcgcccg gcgttctaag caccatcaga acggacctcg catctcatcc
     3361 ggcttgcggg ctggctgtgg cgcggccggc agcgcgccga cacgccaccg tcgcgccgat
     3421 cgcgcgacgg gaactcctac ttcaagtctg acatgccatc aggaccccgg gccaccgtcc
     3481 cggcgccctc ggcctcgaac cggccaagcg cctccggggg gaggtcggtg gtggtgatgg
     3541 tggcggcggc gctgccggcg ccgaggtcca cggcgaggtc gacctggcct ttggcggcga
     3601 ccgcggcggc ggcgcgctcg tcgtgggcgg cggcctcacc gccggtgacc acggtgacgg
     3661 ggccgaagcg gacgccgacc cgggcggggt cgagggggat cgcggcccgg tcgatcgcgt
     3721 cgagcaccgc gggccaggcg ggcaggccgc tggcgagggc ggcgcgcagc gcgatcgagg
     3781 cggcgaccgc gcgggcggcg gccagcgcgt cggcgtggct ggcggcgccg tggacggtga
     3841 cgacgatcag ctttcggctg ccgggcacgc gcatggcgag ctgctcgccg agggattcgc
     3901 agaggtaggc gagggcggcc tcgaacgcgg cggcaccttc ggagccggcg gtgagcaccg
     3961 gcgcggcggc ggtggcgttg gcgagcagga tggcgctgtc ggccatgctg gggccctggt
     4021 cgaccaggat tcgctcgaag gagcggccga gggcccgttc gaggatgccg tcgagcaccg
     4081 gcggctcgac ggtggcgtcg gtggtgagca ggaccagggt gctggcgaag gagggcgcga
     4141 acggaaccga ggccttggcg accccgccga tgcggacctc cccttcggac caggcgacgg
     4201 cgacggcgtg ttcccgggcg acgccgccgg aggcggccag ggcgcgggcc agctcggcgg
     4261 agccgcggcg gccgagcgcg gtgacggcct tctcggcgcc ttcggcggcg gcttccagtg
     4321 ggaggcggcg gccgatcggg ccggtgccga gcaggaggac ctcggcgggg gcgcagccga
     4381 cggcggtggc gcaggcctcg gcgagggcgg ccgcgtcggc gtcgccgcgg gcgccggtgg
     4441 cggcgttggc ggcgccggcg tggaccagga ccgcgcgggc gctgccgtcg gcgagctggc
     4501 gccgtgacca gcggactggc gcggcggcga agcggtggct ggtgaagcgg ccagaggtcg
     4561 ccgcggtctg ctggctcacc accaggccga ggtcggaccc gggtccggcg accagcccgg
     4621 cgtcgacccc tgccgcgcgg aacccccggg gtgcgcaaac cccgccctcc accccggcga
     4681 ccgtgtagtc gaccacaacc acctcccttg tcagccgtca gcgccgacaa tccacgcctt
     4741 cggtgcatcc tgggcgttct ggcatggccc ccgcgcgctg ggggcccccg ctgggggtcc
     4801 ccccggtttc aagcctacgg cgcggcgggg tcgctcggca tcccccacag ccacatctgt
     4861 ggataaccag ctgtcccctg aaagggccga cgggtggctt tgccacccgt caaccccctt
     4921 gaccaccgtg gcggccacgc caagaggggg agcctcgagg gggagctgcg ctccccctcg
     4981 attcacgcgc ggagggtggc gtcgtggcgt tcggcggcca gggcgaccat gcggtcgcgg
     5041 accgcggcgg catcggcgtc ggtgagggtc cggtcggggg cctgcagggc gacccggtag
     5101 gcgaggttgc ggtatcccgg cggcagcggc gctccctggt aggcgtcgaa caacgccagg
     5161 cgggcgagca gctccgcgcc agcctcgctg agggtcgcct gcaggcccgc ggcggcgacg
     5221 ccgggtggga ccaggaaggc cacgtcgaag ctcagctccg ggtagggcga cggggtcatg
     5281 gccggccggg tggcgggggc cgcctccagc agcggggtca gctccagctc ggcggc
//
LOCUS       DASWDC010000061         6028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_590979, whole genome shotgun sequence.
ACCESSION   DASWDC010000061 DASWDC010000000
VERSION     DASWDC010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590979"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VF995_02020"
     CDS             <1..850
                     /locus_tag="VF995_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX9342378.1"
                     /translation="APTPDWLSFFMFTFFTDRDGKFQLASLAESAFDPLSRTVQFMLR
                     EEAHHMFIGTTGIARVIQRTCQLMRQHRAEDPEAVRRVGGIDLPLLQKYLNLHYSVSL
                     DLFGGELSSNAASYFSSGLKGRFQETRRSDDHRLVEATYPVSQLEGGRVETVQAPALN
                     AMNECLRDDYIADCQRGVNSWNKLIARAGIEFELRLAHRGFNRHVGRFADAHVAPDGG
                     LLGGAAWQEQVDQWLPTPADEEYVASLMVPVYEPGKMANWIAPPATGINDKPVDYEYV
                     RFEQRP"
     gene            868..2472
                     /locus_tag="VF995_02025"
     CDS             868..2472
                     /locus_tag="VF995_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX9342379.1"
                     /translation="MTAVELPPEVATGVPERLNACEYLLDRRLEAGDGERVALQERGG
                     ATLTYREVAARVARVAGALRALGVRPEERVPLALLDGAEFVASFLGALRIGAVPVPMS
                     TMLTGAELAFQCRDARARVAVASAALGPALAEVAEGSGELADLVLAGDAPVVIPATVR
                     THRFDEWLTGPDPWQAAPAYGSWPDSPAFWLYTSGTTGKPKAAMHRHADLPYTAETYG
                     RQVLGIGPDDRCYSVAKLFFAYGLGNALTFPFSVGASAVLDPGRPTPAGVAETVRSTR
                     PTLFFAVPTFYAALLAAEADGAVPRDTFASVRLGVSAGEPLPAGLCTRLKDRFGLDVL
                     DGLGTTEALHIFLSNRPGEIRPGTTGRVVPGYRVRLVDDDGAAVPPGSGGAGHLLVAG
                     DSVATGYWCRTEVTRRTFQGEWLRTGDVYACDDDGVFTYQGRSDDMIKAGGIWVSPAE
                     VEATLIQHPDVLEAAVVARRTPDGIEEPAAFVVCLPGRAVAADELVAFCRERLAAFKR
                     PRQVFFTDELPKTATGKIQRFKLRGA"
     gene            complement(2545..2754)
                     /locus_tag="VF995_02030"
     CDS             complement(2545..2754)
                     /locus_tag="VF995_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX9342380.1"
                     /translation="MMAVGTVKWFNADKGYGFISPESGEDVFVHFSAIQSRGYRSLNE
                     GQAVEFDITQGPKGAQAANVRPIET"
     gene            complement(2915..4081)
                     /locus_tag="VF995_02035"
     CDS             complement(2915..4081)
                     /locus_tag="VF995_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9342381.1"
                     /translation="MATVTGALDLGALTLVDHHCHGIVLDDLERPAFERLITEGDPPA
                     AEEGAFDSPVGLAIRRWCAPVLGLEPHVPAGRYLERRAELGAPAASRLLLRASGTGTL
                     LLDTGLRPGEILDPAAMGELAGAATRELVRLEAVAEALAAERPPAAEFPDRFAARLRD
                     TVKDAGDGAAGLKSIVAYRLGFDFDPAPPARAEVVAAAGRWLRDLGAVPGAPRVTDPV
                     LLRHGIWAGAELARERGLPLQFHAGFGDTDLDLRRADPLHLTGLIRRFRELGVNLVLL
                     HCYPFHRHAGYLAAAYRNVFFDVGLALSYSGARAAAILAESLELAPFGKLLYSSDAFG
                     LAELYLLGATHFRRALHQVLDGWVASGDCAVPDAAAVAQRIAASNAERLYRLHP"
     gene            complement(4430..5680)
                     /locus_tag="VF995_02040"
     CDS             complement(4430..5680)
                     /locus_tag="VF995_02040"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9342382.1"
                     /translation="MGEGPARRGAVALLAQRDFGLYAVGSLLSNAGNFCQSIALSLLV
                     FRLTRSTLLVGVVNFAQYAGVLLLAPLAGAAADRYERRRLLVASQLAAVLISGALALL
                     VAAGRAGVGSVMVLAALLGVTTAFCFPAQKALVPALVEPADLHRAVGMDSMTMNLSKA
                     LGPALGALLVLRVGFAWAFAANCASYLALIVAVLVIRPRPQASGPGRDDDAAGGRGGG
                     VLGALRVLRGEPELVLLLAVVAVLAIASDPAPTLGPGFAVHLYGRSDTVAGLLFGTFG
                     IGAVLGALTIARPAARPYRRMALLLVGMAVANAAFALSGAGTLWLGLAGLAVAGGTYL
                     AAQTQATTMIHESLDDARRGRVMALWTVAFVGTRPLASLADGALATVVGLRGAALLIS
                     LPLLAVALLVPVARRRQAERRASR"
BASE COUNT          869 a         2165 c         2228 g          766 t
ORIGIN      
        1 cgcgcccacg ccggactggc tgtcgttctt catgttcacc ttcttcaccg accgggacgg
       61 caagttccag ctcgcctccc tggccgagag cgccttcgac ccgctgtcgc gcacggtcca
      121 gttcatgctg cgcgaagagg cgcaccacat gttcatcggc accacgggga tcgcgcgggt
      181 gatccagcgc acctgccagc tgatgcgcca gcaccgcgcc gaggaccccg aggcggtgcg
      241 ccgcgtcggc gggatcgacc tgccgctgct gcagaagtac ctcaacctcc actacagcgt
      301 ttcgctggac ctgttcggcg gggagctgtc gtcgaacgcg gcgagctact tctccagcgg
      361 tctcaaggga cgcttccagg agacccggcg cagcgacgac caccgcctgg tggaggcgac
      421 ctacccggtt tctcagctgg agggcgggcg ggtcgagacc gtgcaggctc cggcgctgaa
      481 cgccatgaac gagtgcctgc gcgacgacta catcgccgac tgccagcgcg gcgtcaactc
      541 ctggaacaag ctgatcgcca gagcggggat cgagttcgag ctgcggctgg cccaccgcgg
      601 cttcaaccgc cacgtcggca ggttcgccga cgcccacgtc gccccggacg gcggcctgct
      661 cggcggggcg gcatggcagg agcaggtgga ccagtggctg ccgacccccg ccgacgagga
      721 gtacgtggcg tcgctgatgg tgccggtcta tgagccgggg aagatggcta actggatcgc
      781 cccgccggcg accggcatca acgacaagcc ggtggactac gagtacgtgc ggttcgagca
      841 gcggccctga tccttgggga gggtgggatg acagcggtcg agctgccacc ggaggtggcc
      901 acaggggtcc ctgagcggct caacgcctgc gagtacctgc tggaccggcg actggaggct
      961 ggggacgggg agcgggtcgc cctccaggag cgcggtgggg cgaccctcac ctaccgggag
     1021 gtcgcggcgc gggtggcgcg ggtcgcgggc gcgttgcggg cgctcggcgt gcggcccgag
     1081 gagcgggtgc cgctggcgct gctggacggg gccgagttcg tcgccagctt cctcggcgcg
     1141 ctgcggatcg gggcggtgcc ggtgccgatg tcgaccatgc tgaccggcgc cgagctggcc
     1201 ttccagtgcc gcgacgcgcg cgcccgggtg gcggtcgcct cggcggcgct cggcccggcg
     1261 ctggcggagg tggccgaggg ctcgggcgag ctggccgacc tggtgctcgc cggggacgcg
     1321 ccggtggtca tccccgcgac ggtgcggacc caccgcttcg acgaatggct cacgggaccg
     1381 gatccgtggc aggcggcgcc cgcctacggg agctggccgg actcgcccgc gttctggctc
     1441 tacacctcgg gcaccaccgg caagcccaag gcggccatgc accgccacgc cgacctgccc
     1501 tacaccgccg agacctacgg ccgccaggta ctcgggatcg gcccggacga ccgctgctac
     1561 tcggtcgcca agctgttctt cgcctacggg ctcggcaacg cgctgacctt cccgttctcg
     1621 gtcggcgcca gcgcggtgct ggacccgggg cgtcccaccc cggccggtgt cgccgaaacg
     1681 gtgcgctcca cccgtccgac gctgttcttc gcggtgccga cgttctacgc ggcgctgctg
     1741 gccgccgagg ccgacggtgc ggtgccgcgc gacaccttcg cgtcggtgcg cctcggcgtg
     1801 tcggccggcg agccgctccc ggcgggactc tgcacccgcc tgaaggaccg cttcgggctg
     1861 gacgtgctcg acggcctcgg gaccaccgaa gcgctgcaca tcttcctctc caaccggccg
     1921 ggcgagatcc gcccggggac gaccggccgg gtggtgcccg gctaccgggt tcgcctggtc
     1981 gacgacgacg gcgcggcggt gccgcccggc tcgggcggag ctgggcacct gctggtcgcg
     2041 ggtgactcgg tggcgaccgg gtactggtgc cgcaccgagg tgacccgccg gaccttccag
     2101 ggcgaatggc tgcgcaccgg ggacgtctac gcctgcgacg acgacggggt gttcacctat
     2161 cagggccgct ccgacgacat gatcaaggcc ggggggatct gggtctcccc cgccgaggtg
     2221 gaagccaccc tgatccagca cccggacgtg ctggaggccg ccgtggtcgc ccgccgcacg
     2281 cccgacggca tcgaggagcc cgccgccttc gtggtctgcc tccccggccg cgccgtggcc
     2341 gccgacgagc tggtcgcctt ctgccgcgaa cgcctcgcag ccttcaagcg gccccgccag
     2401 gtgttcttca ccgacgagct gcccaagacc gccaccggca agatccagcg cttcaagctg
     2461 cgcggcgcct gaggcgtccg gatacgcggt ggccccaccc gggctgcccg ggtggggcca
     2521 ccactgacga ccgtcagggc cgaactacgt ctcgatcggc cgcacgttcg cggcctgtgc
     2581 gcccttcgga ccctgggtga tgtcgaactc gacggcctgc ccctcgttca gcgagcggta
     2641 gccgcggctc tggatcgcgc tgaagtggac gaagacgtct tcgcccgact ccgggctgat
     2701 aaagccgtag cccttgtcag cgttgaacca cttgacggtt ccaacagcca tgattgtttt
     2761 gtccctccct ccgtttctcc ggaggtatag caactcacca gcaccaacgg caccggaggc
     2821 aagggacagc tcgactgctg agtgaagcca aacattgcgt ctcctgcgcg ccagagggta
     2881 gcacgttggc gccctcacgt ggcgcaaaag cttatcaggg gtggaggcgg tagaggcgtt
     2941 cggcgttgct ggcggcgatc cgctgggcga cggcggcggc gtcgggaacg gcgcagtcgc
     3001 cgctggcgac ccagccgtcg aggacctggt ggagggcacg gcggaaatgg gtcgcgccga
     3061 gcaggtacaa ctcggccagg ccgaacgcgt cggagctgta gagcaatttg ccgaagggcg
     3121 ccagctccag cgactcggcc aggatcgccg ccgcccgggc gccgctgtag gagagcgcca
     3181 ggccgacgtc gaagaagacg ttgcggtagg cggcggcgag gtagcccgcg tggcggtgga
     3241 acgggtagca gtgcagcagc accaggttga cgcccagctc gcggaaccgg cggatcaacc
     3301 cggtcaggtg gagggggtcc gcgcggcgca ggtccaggtc ggtgtccccg aacccggcgt
     3361 ggaactgcag cgggaggccc cgctcgcggg ccagttccgc gcctgcccag atcccatggc
     3421 gcagcagcac cgggtcggtg acgcgcgggg cgcccggcac ggctcccagg tcacgcagcc
     3481 agcggccggc ggcggcgacc acctcggcgc gagcgggcgg tgccgggtcg aagtcgaagc
     3541 cgagccggta ggcgacgatg ctcttcaatc cggcggcgcc gtcgcctgcg tccttgaccg
     3601 tgtcgcgcag gcgcgcggcg aagcggtcgg ggaactcggc ggcgggcggc cgctcggcgg
     3661 cgagcgcttc ggcgaccgcc tccaaacgga cgagctcgcg ggtggcggcg ccggccagct
     3721 cgcccatcgc ggccgggtcg aggatctcgc cgggccgcag gccggtgtcg agcagcagcg
     3781 tgccggtgcc gctggcgcgc agcagcagcc tgctcgccgc cggggcgccc agctccgcgc
     3841 gccgctccag gtagcgcccg gccgggacgt gcggctccag gcccaggacg ggggcgcacc
     3901 agcgccggat cgccaggccg accgggctgt cgaaggctcc ctcctcggcc gccggcgggt
     3961 cgccttcggt gatcagccgc tcgaacgcgg gccgctccag gtcgtcaagg acgatcccgt
     4021 ggcagtggtg atcgaccagg gtgagcgcac ccaggtcgag cgcgccggtg acagtcgcca
     4081 tgcagcgatt atgcggcgct tggcgccgat tggcgggaag tcgccgtggg gctggagggc
     4141 caacgggccg cggtcgggat gcggcgctgc cgggcccccc ggcggtagga gcccggcagc
     4201 gcacgggggg cggtcaggga ctgcgggtca cggtggcgag cgcgaggacg gcgacgccga
     4261 ggacggccat cgcaagcgcg atcagcagcg tggacattgg ccgccccttc cgagcaagtt
     4321 tcgcaacttc gcgacttgga tctactgctc tatgcggtag catccgccaa cccggttacg
     4381 accgaagcac ggccgggcat cggctctgga acagcgtgcc agccctgctt cagcggctgg
     4441 ctcgccgctc ggcttggcgc cgtcgcgcga ccggtaccag cagcgcgacg gccagcagcg
     4501 gcaggctgat cagcagcgcg gcgccgcgca ggccgaccac cgtggcgagg gcgccgtcgg
     4561 cgaggctggc gagcgggcgg gtgccgacga aggcgaccgt ccacagcgcc atcacccttc
     4621 ccctgcgggc gtcgtcgagc gactcgtgga tcatcgtggt ggcctgcgtc tgggcggcga
     4681 gataggtgcc gcccgcgacc gccagcccgg cgagccccaa ccacagcgtc ccggcgccgg
     4741 agagcgcgaa agcggcgttg gccacggcca tcccgaccag cagcagcgcc atacgccggt
     4801 agggccgggc ggccggccgc gcgatggtga gcgcgccaag taccgcgccg atcccgaagg
     4861 tgccgaacag cagcccggcg acggtgtcac tgcgcccgta gaggtgcacc gcgaacccgg
     4921 gcccgagcgt cggcgccggg tcggaggcga tcgcgagaac cgccaccacc gccagcagca
     4981 gcaccagctc cggctctccc cgcagcaccc tgagtgcccc gaggactccc ccaccacggc
     5041 ctccagccgc gtcgtcgtct cggccagggc cgctcgcctg aggacggggc cggatcacca
     5101 gcaccgccac gatcaacgcc aggtaggagg cacagttggc ggcgaacgcc caggcgaagc
     5161 cgacccgcag caccagcagc gcccccagcg cgggcccgag cgccttggac aggttcatgg
     5221 tcatcgagtc catcccgacc gcccggtgca ggtccgccgg ctccaccagc gccgggacca
     5281 gcgccttctg cgcggggaag cagaacgcgg tggtcacccc gagcagggcg gccagcacca
     5341 tcaccgagcc gacgccggcg cgcccggcgg ccaccagcag cgccagcgcg cccgagatca
     5401 gcaccgccgc gagctgactg gcgaccagca gccgccgccg ctcgtagcgg tcggcggcgg
     5461 ccccggcgag cggcgcgagc agcagcaccc cggcgtactg ggcgaagttg accaccccga
     5521 ccagcagggt cgagcgggtg aggcggaaca ccagcagcga cagcgcgatc gactggcaga
     5581 agttgcccgc gttggacagc agcgacccga ccgcgtacag cccgaagtcc cgctgggcga
     5641 gcagcgccac cgccccgcgc cgcgccgggc cctcccccac cggcgcgggc gccaggctcg
     5701 cgggactcgc ggagctgaaa tcaccggtta tacgcatacc gagacaatgt agcggtccga
     5761 gggtcctggc cgcggtctcg tctcatgccg tgcgagctga ccttctcctg ctggggttgc
     5821 cggggccgag gccgaggggg aggcctgctg acgcacggcg aacagggccg cgaacgctga
     5881 gccctcgacc agcccggcgc caaagcgcgc cgctgggagc gcctctacgg gagcggagcg
     5941 cccccgccgg aggcgctggc ggcggacgag ttgccggcct gcatgtccag cgcctggagg
     6001 accttggtgc ggtcgaggcc ggggatgt
//
LOCUS       DASWDC010000062         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_263294, whole genome shotgun sequence.
ACCESSION   DASWDC010000062 DASWDC010000000
VERSION     DASWDC010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263294"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="VF995_02045"
     CDS             <1..1395
                     /locus_tag="VF995_02045"
                     /inference="COORDINATES: protein motif:HMM:NF045160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342383.1"
                     /translation="GSLDRAELVALLIEQAGTDEALQHKLTLRQARGAGGRSGPDAAA
                     LRKQVNTALRVRGFVDYRGSYDYSERASEVLDVLDDVLDSGHAAVVVPLAQRALELLV
                     KATLQMDTSSGAPGEACERALDLHARACAQAPPDPVKLAHWLVDLEVEGPGWPDVDLA
                     IYQDGLGEAGIAAYRARVEELWAALPPNPRTPGRVGMHLYGHDDHRRYVLNHMMEELA
                     ELDDDVDQLVAVMAADLSSGWQYVRIATTLQEANRAAEALVWAERGLAASEGRPDARL
                     VDVVVGEYLRTGRTAEAIALRRAALEQYPSQPVYEALRTAAESAGRWPAERASALVLL
                     RKQAAAGGGLANATLIRALLADGETDAAWAAANLKGAPGCTDDLWLTLAEQRAATHPA
                     DAIGVYQQLAERAIDTRNKRGYGQAADLVVRIRDLHRRLDSNAPAFSAYLTDLKARHK
                     PKRNLMAELAGRGL"
     gene            complement(1406..1795)
                     /locus_tag="VF995_02050"
     CDS             complement(1406..1795)
                     /locus_tag="VF995_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018790147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX9342384.1"
                     /translation="MSDTPVLDIPASDSYQAHRQRLLDAEQRLRDQREQVAELRRALP
                     PGPVIDTDYVFREGDPDLNVESADSERDTRLPDLFAPDGTIRLSYAGSPYYGKDEYRG
                     IDLLSPVWHLLDLLPTGRGDWMPSDGH"
     gene            1898..2218
                     /locus_tag="VF995_02055"
     CDS             1898..2218
                     /locus_tag="VF995_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017946642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9342385.1"
                     /translation="MDAIQVVAEPRRREILRLVWEGERSASEIAAQFGVTFGAVSQHL
                     GVLRRAGFVLVRHDGPRRFYRADRAALGPLATLLEQMWASQLDQLAALAGAEAEATRQ
                     REAG"
     gene            2215..>2561
                     /locus_tag="VF995_02060"
     CDS             2215..>2561
                     /locus_tag="VF995_02060"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9342386.1"
                     /translation="MSERGPAPVVTVERYVKAPPETVFTFFADPSRWLQWQGVHAQIE
                     PRPGGRFRMDVMGDGYASGRFVEVTPPRRVVFTWGYEAPGSPVPPGSSTVEIDLLPDG
                     DGTLVRLTHRGLPT"
BASE COUNT          359 a          939 c          916 g          347 t
ORIGIN      
        1 gggtcgctgg accgagcgga gctggtggcg ctgctgatcg agcaggctgg cacggacgag
       61 gccctgcagc acaagctcac gcttcggcag gcgcggggag cgggaggccg gagcggcccg
      121 gacgccgccg cgctgcgaaa gcaggtcaac accgcgctgc gggtccgcgg gttcgtcgac
      181 taccgcggca gctacgacta ctcagaacgc gccagcgagg tcctcgacgt gctcgacgac
      241 gtcctcgaca gcgggcacgc agcggtggtg gtcccgctcg cgcagcgggc gctggagctg
      301 ctcgtcaagg ccaccctgca gatggacacc tccagcggcg cgccgggcga ggcctgcgag
      361 cgcgcgctcg acctgcacgc ccgtgcctgc gcccaggcgc cgcccgaccc ggtgaaactc
      421 gcccactggc tggtggacct ggaggtcgag ggccccggct ggccggacgt ggacctcgcc
      481 atctaccagg acggcctcgg cgaggccggg atcgccgcct accgcgcccg ggtcgaggag
      541 ctgtgggccg ccctgccgcc caacccacga acgcccggca gggtcggcat gcacctctac
      601 ggccacgacg accaccgccg ctatgtcctg aaccacatga tggaggagct cgccgagctg
      661 gacgacgacg tcgaccagct ggtggcggtc atggcggcgg acctgtcatc cggctggcag
      721 tacgtgcgga tcgccaccac cctgcaggag gcgaatcgtg ccgccgaggc gctggtgtgg
      781 gccgaacggg gcctcgccgc cagcgagggt cgccccgatg cgcgcctggt cgacgtcgtc
      841 gtcggcgagt acctgcgcac cggccggacc gccgaggcga tcgccctccg ccgcgccgcc
      901 ttggagcaat accccagcca gccggtctac gaggcgctgc gcacggcggc ggagtctgcc
      961 ggtcggtggc cggcagagcg cgcctcggct ctcgtcctgc tgcgcaagca ggccgccgcc
     1021 ggtggcgggc tcgccaacgc gacgctgatc cgggcgctcc ttgccgacgg tgagaccgac
     1081 gccgcctggg ctgccgctaa cctcaaggga gcgccgggat gcaccgatga cctctggctc
     1141 acgctcgccg agcagcgggc ggcaacccac cccgccgacg ccatcggcgt ctaccagcag
     1201 ctcgccgagc gcgccatcga cacccgcaac aagcggggct acggccaagc cgccgacctg
     1261 gtcgtgcgga tcagagacct gcaccgccgc ctcgactcga acgcccccgc cttcagcgcc
     1321 tacctgaccg acctgaaggc acgccacaaa cccaagcgca acctgatggc cgaactcgcc
     1381 ggccgcggcc tgtagccgca gtggttcagt ggccgtcgga gggcatccag tcgccgcggc
     1441 cggtggggag caggtcgagc aggtgccaga cgggggagag caggtcgatg ccgcggtatt
     1501 cgtccttgcc gtagtagggg ctgccggcgt aggagaggcg gatggtgccg tcgggggcga
     1561 acaggtccgg caggcgcgtg tcccgctcgc tgtccgcgct ctcgacgttc aggtccgggt
     1621 cgccttcgcg gaagacgtag tcggtgtcga tcaccgggcc cggcggcagg gcgcggcgca
     1681 gctccgccac ctgctcgcgc tggtccctga ggcgctgctc ggcgtcgagg agccgttggc
     1741 ggtgcgcctg gtagctgtcg ctggcgggga tgtccaggac cggggtgtcg ctcatctcgc
     1801 atcggctcct tcgggtcggc cgccgtgatt ggctttagtg aagactaaaa tgatgctgcc
     1861 cacagatgca agctttagcc actgctaaac tccgagcatg gacgcgatcc aggtcgtcgc
     1921 cgagcctcgg cggcgcgaga tcctgcggct ggtctgggag ggcgagcgtt ccgcctccga
     1981 gatcgccgcc cagttcggcg tcaccttcgg cgcggtctcg cagcacctcg gcgtgctgcg
     2041 cagggccggc ttcgtgctgg tccgccacga cggcccgcgg cgcttctacc gagcggaccg
     2101 tgccgcgctc ggcccgctcg ccaccctgct cgaacagatg tgggccagtc agctcgacca
     2161 gctcgccgcc ctggccggag ccgaggcgga agccacgcgg cagcgggagg cgggatgagc
     2221 gaacgcgggc ctgccccggt cgtgaccgtc gaacgctacg tgaaggcccc gccggagacc
     2281 gtcttcacct tcttcgccga cccctcgcgc tggctccagt ggcagggcgt ccacgcccag
     2341 atcgagcccc gcccgggggg gcggttccgc atggacgtca tgggcgacgg ctacgcctcc
     2401 ggccgtttcg ttgaggtcac cccaccccgt cgcgtggtct tcacctgggg ctacgaggcg
     2461 ccgggcagcc cggtcccgcc ggggtcgagc accgtcgaga tcgacctgct ccccgacggc
     2521 gacggcaccc tggtccgcct cacccaccgc ggcctgccca c
//
LOCUS       DASWDC010000063         5576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_340551, whole genome shotgun sequence.
ACCESSION   DASWDC010000063 DASWDC010000000
VERSION     DASWDC010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340551"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VF995_02065"
     CDS             <1..397
                     /locus_tag="VF995_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9342387.1"
                     /translation="SAIVHVLTARRIDEDLANRLEHETITAPHLLDVEVLQALRGLTL
                     GHKISHERANHARALYRQLFIVRYPADGLAERIWSLRHNLTAYDAAYVALAEALDCPL
                     VTRDAKLASAGHSANVHIYPRLSPANGDT"
     gene            594..1790
                     /locus_tag="VF995_02070"
     CDS             594..1790
                     /locus_tag="VF995_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX9342388.1"
                     /translation="MAVTARVEHVGSLLRPSYLLEARAAREDGRLGPAAFKAVEDQAV
                     KEVLALQRDVGCPVVTDGELRRDSFQSELSAAADGFAGVDMDAWLWGEWHSGAEGPGD
                     LTVKRPEALGVVAPVRARRRLAAEEFTFTRAVLGDDPDRVAKVTLPSPSLFANLWSPA
                     EHAGERRNAVHGRAAEAKTELSRGAYPTLDAFLADVVGVLQDEVRELVRLGCTYIQLD
                     APHYPLLIDPHWQAFYEARGWPLERWLSYGVELDNAVIDAGRPATFGFHLCRGNQRSR
                     WLVAGGYDAIAGPIFGGVHADRLLLEYDDERSGSFDPLRLVPDDKWVVLGLVTTKTPR
                     VETEADLERRVGDASRWIGRDRLAISPQCGFATSYLGNAISIEDERRKLAVLVRTAER
                     VFGGVR"
     gene            1870..3453
                     /locus_tag="VF995_02075"
     CDS             1870..3453
                     /locus_tag="VF995_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9342389.1"
                     /translation="MRATATDADVLIVGAGPTGLLLAGDLAGAGVACTVLERRVEDSS
                     LTRAFAVHARTLEELDARGVADELVETGATVGSLAVFGGVRLALSQLETRFPFVLVTP
                     QYHTERLLAERARALGAELVRGAEVTGLRQDTGGVDLEVRTKAAGDGEWVAGTRRASY
                     VVGTDGVRSTVRHALGLPFPGRSIARSLMLADVRLAETPPGVLTANGTGRSFAFIAPF
                     GDGWYRVIAWNRRHQVPDSEPVDFDELRAVTRDALGTDYGMRDPRWTSRFHSDERQAP
                     TYRVGRVFLAGDAAHVHSPAGGLGMNTGLQDAANLGWKLAAAVQGSAPDGLLDSYQRE
                     RYPVGTQVLRTSDALLRAAFAQSRALRAVRGVAAALATRIKPVADHFAGRITALGVSY
                     PAPHGAHPLTGKRAADVPLAVTADDSGEQTPGPRSDRLVPPRLYEALRGGQFVLLAPA
                     LGTEAAARLRDAVAPWIDRVDVATPATATSPPTWLLVRPDAYVAWASNEPDPAAGTEA
                     LRAALASWCGAPTPAQAET"
     gene            3600..4292
                     /locus_tag="VF995_02080"
     CDS             3600..4292
                     /locus_tag="VF995_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX9342390.1"
                     /translation="MNPFRGRLLRAAAVILPVAAAAGWYLAPMSAHASSQVAVWQMNE
                     GAGATTMVDSAGGHDGAVKNAQTGVPGFSGNAYRFNGSSSVVVVPASPAFNPGSADWS
                     FTVHVNFTKPPNATVGDYDLFRGPSQGAYKAEILARKNRTQGVASCFFKGSSTKATLT
                     AGPNLADGRWHTITCTKHASNIQLVVDGTTFTRTVTIGTLTNTGPVSLGAKAKGGDWY
                     DGLLDEVSVTSG"
     gene            4377..5015
                     /locus_tag="VF995_02085"
     CDS             4377..5015
                     /locus_tag="VF995_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX9342391.1"
                     /translation="MQAPPYGAYPVQFSVDYPDRPLDRLTSAFRILVAIPILILIGAV
                     SGNAWQWSAGNGTTRTIGGAGGLLFFGPLLMLVFRRKYPRWWFEWNLELLRFSNRVWA
                     YLALMDDHYPSADEQQAVHLEIPYPDAQRLNQWLPIFKWLLAIPHYIVLFFLGIGAFV
                     ASVIAWFAILFTGTYPRGLFHFVEGTFRWTNRVIGYALILVTDEYPPFRLNP"
     gene            5061..>5576
                     /locus_tag="VF995_02090"
     CDS             5061..>5576
                     /locus_tag="VF995_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX9342392.1"
                     /translation="MATHEVRVDYAKTLAEEPETGHNRWHPDIPPIVRCDPGDEVILQ
                     TRDALDGQAGPRAALEAVGAADLNVVHPLTGPVSVSGAEPGDILEAEILRIEADRYGF
                     TVQLPGFGFLRDEFPDPFMVRWAIADGHATSDDLPGVRIPGAPFMGTIGLSPDRRLME
                     VCAAREQAAIDR"
BASE COUNT          795 a         2061 c         1917 g          803 t
ORIGIN      
        1 ctctgcgatc gtccatgttc tcaccgccag gcgcatcgac gaggatctcg cgaaccgctt
       61 ggagcacgag accatcaccg cgccccacct tctcgacgtc gaggtcctac aggccctgcg
      121 cggcctgacg ctgggacaca agatcagcca tgagcgagcc aaccacgcgc gggccctcta
      181 ccgccagcta ttcatcgtcc gctacccggc cgacgggctc gccgagcgga tctggagtct
      241 gcgtcacaac ttgactgcct acgacgccgc ctacgttgcg ctggccgaag cgcttgactg
      301 tcccctcgtc acccgcgacg ccaagctcgc cagcgcaggc cacagcgcga acgtccatat
      361 ctacccgagg ttgagtcccg ccaacgggga tacgtgacac ggtcactgac cttccaccga
      421 gccttgaccg gggggcgagc gtaggtgccc gatcgcgagc gcgtcgaccc tgggtcggcc
      481 aaggcgccca tctcgcggac gggaccggcc gccgcagcga acaacgcatc agcggtgacg
      541 tgacccggta acggcaccaa caccgaaccg gcggggtccg ctaccgtgag gttatggctg
      601 ttacggcgcg agtcgagcac gtcgggagtc tcttgcgtcc ctcctacctg ctggaggcgc
      661 gggcggcgcg ggaggacggg cggctgggcc cggcggcgtt caaggcggtt gaggaccagg
      721 cggtcaaaga ggtgctcgcc ctccagcggg acgttggctg tccggtggtc accgacgggg
      781 agctgcggcg cgactcgttc cagagcgagc tgtcggcggc cgccgacggg ttcgcggggg
      841 tcgacatgga cgcctggctg tggggcgagt ggcactccgg cgccgaaggg cctggcgatc
      901 tgacggtgaa gcggcccgag gccctcgggg tggtcgcgcc ggtgcgggca cggcggcggc
      961 tcgcggccga ggagttcacc ttcacacgcg ctgtcctcgg cgacgacccg gaccgggtgg
     1021 cgaaggtgac gctgccgagc ccgagcctgt tcgccaatct ctggtcgccg gccgagcatg
     1081 caggcgagcg acgcaatgct gtccacggga gggcggcgga ggcgaagacg gagctatccc
     1141 ggggtgccta cccgaccctg gacgccttcc tcgccgacgt ggtcggggtt ctccaggacg
     1201 aggtccggga gctggtgcgg ctcggctgca cctatatcca gctcgacgcg ccgcactacc
     1261 cgctgctgat cgacccgcac tggcaggcgt tctacgaggc gcggggttgg ccgctggagc
     1321 gctggctctc ctatggcgtc gagctggaca acgcggtgat cgatgcgggc cgcccggcca
     1381 ccttcgggtt ccacctctgc cgcggcaacc agcgcagccg ctggctggtc gcgggcggct
     1441 acgacgccat cgccgggccg atcttcggcg gcgtccacgc cgaccgccta ctgctggagt
     1501 acgacgacga acgatccggc agcttcgacc cgctgcgcct ggtccccgac gacaagtggg
     1561 tggtcctcgg cttggtcacc accaagacgc cccgggtcga gaccgaggcg gaccttgagc
     1621 gccgggtggg tgacgcgagc cgctggatcg ggcgggaccg cctggcgatc agcccgcagt
     1681 gcggcttcgc cacctcctac ctcggcaacg ccatcagtat tgaggacgag cgccgcaagc
     1741 tcgctgtcct ggtccgcacc gccgagcggg tctttggcgg cgtccggtag gacccggttg
     1801 cgccgcctta cggccgggta agcgtgccct tggggcgcgt ttcgggggga gtagtaggag
     1861 gtgacgagga tgcgcgccac tgccacagac gccgacgtgc tgatcgtcgg ggccggcccg
     1921 acggggctgc tgctcgccgg ggacctcgcc ggggccgggg tcgcctgcac ggtgctggag
     1981 cggcgggtcg aggactccag cctcacccgc gcgttcgcgg tccatgcccg cacgctggag
     2041 gagctggacg cccgcggcgt cgccgacgag ctggtcgaga ccggggcgac ggtggggtcg
     2101 ctggcggtct tcggcggcgt caggctcgcc ctctcccagc tggagaccag gttcccgttc
     2161 gtgctggtga cgccgcagta ccacaccgaa cggctgctcg ccgagcgggc cagggcgctc
     2221 ggcgccgagc tcgtgcgggg cgccgaggtg accgggctgc gccaggacac cggcggcgtc
     2281 gatctggagg tccgcaccaa ggccgccggc gacggcgagt gggtggccgg cacgcggcgg
     2341 gccagctacg tggtcggcac cgacggggtc cgcagcaccg tgcgccacgc gctcgggctg
     2401 ccgttcccgg gccgctcgat cgcacggtcg ctgatgctgg ccgacgtgcg cctggccgag
     2461 acgccgccgg gcgtgttgac tgccaacggc accggcagga gcttcgcctt catcgcgccg
     2521 ttcggcgacg gctggtaccg cgtgatcgcc tggaaccggc gccaccaggt ccccgacagc
     2581 gagccggtcg atttcgacga gctgcgcgcg gtcacccgcg acgccctcgg caccgactat
     2641 ggcatgcgcg acccgcgctg gacctcgcgg ttccacagcg acgaacgcca agcccccacc
     2701 tatcgggtcg ggcgggtgtt cctcgccggg gacgccgcgc acgtgcactc gccagcgggc
     2761 ggcctcggca tgaacaccgg cctgcaggac gccgccaacc tcgggtggaa gctggcggca
     2821 gccgtccagg gctcggcgcc ggacggcctg ctcgacagct accagcgcga gcgctacccc
     2881 gtcggcacgc aggtcctgcg caccagcgac gccctgctgc gggccgcgtt cgcccagtcg
     2941 cgggcgttgc gcgccgtccg tggcgtggcc gccgcactgg cgacccggat caaaccggtc
     3001 gccgaccact tcgcgggcag gatcaccgcc ctcggcgtca gctaccccgc cccgcacggc
     3061 gcccatccgc tgaccggcaa acgcgcggcg gacgtcccgc tcgcggtcac cgccgacgac
     3121 agcggcgagc agacccccgg gccacggtcg gaccgcctgg tcccgccgcg gctctacgag
     3181 gccctgcgtg gcgggcagtt cgtgctgctc gccccggccc tcggcaccga ggcggcggcc
     3241 aggctccggg acgccgtcgc cccctggatc gaccgggtcg acgtcgccac ccccgcgacc
     3301 gccacctcgc cgccgacctg gctcctggtg cgccccgacg cctatgtcgc ctgggcctcc
     3361 aacgagccgg atcccgccgc cggtaccgag gcgctccgcg ccgccctggc cagctggtgc
     3421 ggcgcgccca cgcccgcgca ggcggaaacc tgagcacaac aggtcccgat ggctctcctc
     3481 gcttcggctt cttcaacgac gtaggctgac gtagggctgc tacctcgcct gcggctggcg
     3541 agcggggcga gcgcacggta ccaacgagcc gaccgagtcg ccgagtcaag gttggaccca
     3601 tgaacccgtt ccgcgggcgc ctgctgcgtg ccgccgccgt catcctgccc gtcgcagcgg
     3661 ccgcggggtg gtacctcgcc ccgatgtccg cgcacgcctc cagccaggtc gccgtctggc
     3721 agatgaacga gggcgcgggc gcgaccacga tggtggacag cgctggcggc cacgacggcg
     3781 ccgtcaagaa cgcgcagaca ggcgtgcccg gcttctcggg caacgcctac cgcttcaacg
     3841 gctccagctc ggtggtggtg gtccccgcct cgcccgcgtt caacccgggc agcgccgact
     3901 ggagcttcac cgtccacgtg aacttcacca aaccgccgaa cgccacggtc ggcgactacg
     3961 acctgttccg cggaccttcg cagggcgcct acaaggcgga gatcctggcc aggaagaacc
     4021 gcacccaggg ggtggcgagc tgcttcttca aggggtccag caccaaggcg accctgaccg
     4081 ccggcccgaa cctcgccgac ggccgctggc acaccatcac ctgcaccaag cacgcgtcca
     4141 acatccagct ggtggtggac ggcaccacct tcacccggac ggtgacgatc ggcacgctca
     4201 ccaacaccgg cccggtgtcg ctcggcgcca aggccaaggg cggcgactgg tacgacggcc
     4261 tgctggacga ggtttcggtc acctccggct gacggttgcc tctgacccat ttttccgccg
     4321 gcccgtttgg cttactgtct tgaagggatg cataaaccgg cagaaaggtg gttgcaatgc
     4381 aagcaccgcc gtacggggca tatccagtgc agttctcggt cgactacccc gaccgaccgc
     4441 ttgaccgcct gacctcggcc ttccggatcc tcgtggcgat cccgatcctg atcctgatcg
     4501 gcgccgtctc ggggaacgcc tggcaatggt cggccggcaa cggcaccacc aggacgattg
     4561 gcggcgctgg ggggctgctg ttcttcgggc cgttgctgat gctggtgttc cggcgcaagt
     4621 acccgcgctg gtggttcgag tggaacctgg agctgctgcg cttcagcaac cgcgtctggg
     4681 cctacctcgc cctgatggac gaccactacc cctccgccga cgagcagcag gccgttcacc
     4741 tggagatccc ctacccggac gcccagcggc tgaaccagtg gctgccgatc ttcaagtggc
     4801 tgctggcgat cccccactac atcgtgctgt tcttcctcgg gatcggcgcg ttcgtcgcct
     4861 ccgtgatcgc ctggttcgcg atcctgttca ccggcaccta cccgcgcggg ctgttccact
     4921 tcgtcgaagg gacgttccgc tggaccaacc gcgtgatcgg ctacgcgctc atcctggtca
     4981 ccgacgaata cccgccgttc cgcctcaacc cctagcctca atccctgacc ctggcacgga
     5041 cgaggacgag gagggcaccg atggccacgc acgaggttcg ggtcgactac gccaagacgc
     5101 tcgccgagga gccggagacc gggcacaacc gctggcatcc ggacatcccg ccgatcgtgc
     5161 gctgcgaccc cggtgacgag gtcatcctcc agacccgcga cgccctcgac ggccaggcgg
     5221 gcccgcgggc ggcccttgag gccgtgggcg cggccgacct caacgtggtg cacccgctca
     5281 ccgggccggt ctccgtcagc ggcgccgagc cgggcgacat cctggaggcc gagatcctcc
     5341 gcatcgaagc ggaccgctac ggcttcaccg tgcagctgcc cggcttcggc ttcctccgcg
     5401 acgagttccc cgacccgttc atggtccgct gggcgatcgc cgacggccac gccaccagcg
     5461 acgacctgcc cggcgtccgc atccccggcg cgcccttcat gggcaccatc ggcctgtccc
     5521 ccgaccggcg cctgatggag gtctgcgccg cccgcgagca ggccgcgatc gaccgg
//
LOCUS       DASWDC010000064         7518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_757951, whole genome shotgun sequence.
ACCESSION   DASWDC010000064 DASWDC010000000
VERSION     DASWDC010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757951"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VF995_02095"
     CDS             <1..720
                     /locus_tag="VF995_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX9342393.1"
                     /translation="RGRIITPLKDRFGAEVRTHYPLRLDDEIRLVRQEAMLTWPRSHL
                     GEPVVPDHILEIIARFTRAVREAPQIDARSGVSARFAIAATETAAASAVRRAVLAGEP
                     VPVVRVVDLPAAVPAALGKVEFDELEEGRELELLHHLLKRALAETYRARLAGADVSGL
                     LRRFDDGATVETGELLSAAELLQRIGPVPGLAQLLRRLGDGDAETPGLAAAALEFAME
                     GLHLAKRLGKEELPGRVVYGR"
     gene            723..2696
                     /locus_tag="VF995_02100"
     CDS             723..2696
                     /locus_tag="VF995_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9342394.1"
                     /translation="MSGAYRYGPWRGGPDPLEPPFDLGEAMDRLGERVLAGQRPGEAL
                     NDLLRKGMPGLRGLRDLLRQARDRRRELLRERGRLGGVLDQVRELLDRALDQERQALF
                     PDPSDAARLAEAELDQLPADTARAVRQLADYQWRSPEAAATYEEIRDLLRREVLDTRF
                     RGMRDALRGATSEQLQRVRDMLGELNAMLEADARGEDTSEQFQRFMQRYGDFFPENPQ
                     TLDELIDVLARRSAAASRLLASLSPEQREELAGLMQAAMGDLGLEYELSRLGQALRSR
                     RPDLRWGERAPIGGQQPLGLADATTALEELADLEALEDSLAQDYPGASLDDVDLDAVQ
                     RALGERATADVEALRALERELTEQGYLTRDHGRLRLTPKAIRRIGAAALQRIFRTLEA
                     RDRGDHEIADAGLAGEPTGSSRPWNFGDEQPLDVVRTVKNAVLRQGPATAPGRPRVTL
                     TPDDFEVLDTERRSSAAVCLLIDLSYSMVLRDAWGAAKTTALALHSLSTSRFPQDELT
                     VIGFSAMARTLPAGELAELEPDMVQGTNLQHALLLARRWLARRPEAEPVVLLITDGEP
                     TAHLEPDGVPWFQWPPLPRTLELTLAEVLRLTKQGITINVFMLDSEPRLVRFVQALAR
                     LNHGRVFSPATDRLGDYVVSDYLRARRGRRRSA"
     gene            2860..3327
                     /gene="dut"
                     /locus_tag="VF995_02105"
     CDS             2860..3327
                     /gene="dut"
                     /locus_tag="VF995_02105"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX9342395.1"
                     /translation="MPEVMPSATTGAVDLPLRRLDPELPLPAYAHPGDAGADLRAATG
                     VTLPPGGRAVVGTGVAVAVPEGYAAFVHPRSGLAARNGITVVNAPGTVDAGYRGEVKV
                     ILLNTDPAEPCTIERGDRIAQLVVQPVVQARFLEVAELPATPRGDGGFGSTGR"
     gene            3406..4209
                     /locus_tag="VF995_02110"
     CDS             3406..4209
                     /locus_tag="VF995_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342396.1"
                     /translation="MDQNQRDQQQRPWGERLADEGAAARDEHSVKRLLSDAIGVVRER
                     LGEVSRNGVGPLLEEAAGQVRPVTREELRRRYPGLDDDEVARRLINRAARTAAGVALG
                     IGGMLAAQEALAVVSAGGTLGTLGVSALAEVLVLFIVEAKLRADLGALAGQPARTPKE
                     LVGAVLGEVAAAGGWRSLRGRSLRRAFPEMAARRVAARVVALVPRRFARIIAPEIVAP
                     LVGAAFAARMAARQVRAAGEQCWAALPRTTATWGTPIPSSGNGSAPPRS"
     gene            complement(4281..5123)
                     /locus_tag="VF995_02115"
     CDS             complement(4281..5123)
                     /locus_tag="VF995_02115"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX9342397.1"
                     /translation="MAKDDALVTTDWLAEHLNDPNTRIVEVDEDTDAYGRGHVPGAVA
                     VNWNTDLQDPLRRDFIPPEAFAKLQDSLGITPDTLVVLYGGNNNWFAAYAYWYYKVYG
                     HGPVKLVDGGRKAWELEGRELTSEPPAISATSGYPVRQPDFTIRAFRDEVLARYVNGA
                     GTALVDVRSPEEYRGEKLAPDHLPQESAQRPGHIPGAANIPWLKAVDGTTGKFLSDDD
                     LRGLYGGAGVTADKDVVAYCRIGERSAHTWFVLHELLGYHNVRNYDGSWTEWGSLVGV
                     PIER"
     gene            5480..6043
                     /locus_tag="VF995_02120"
     CDS             5480..6043
                     /locus_tag="VF995_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX9342398.1"
                     /translation="MTMAFVPDRDDALVVVDVQRDFCPGGSLAVPEGDQVVPLLNRLA
                     PRFATVVTTHDTHPPDHSSFIAQGGPWPPHCVEGTLGWERHPDLRVEADYQVLKGRER
                     DQDGYTGWNQELADFLTKRGVRRVVVGGLALDYCVKATALDARAAGHDVVLLTDATRA
                     VNLQPGDGEQALHALRAAGVREDRAEG"
     gene            6206..7354
                     /locus_tag="VF995_02125"
     CDS             6206..7354
                     /locus_tag="VF995_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HEX9342399.1"
                     /translation="MASKAKATSEPDAKSRARGFREHWADEMDGAAMYRALASATGDG
                     EQREIFLELSKAEERHARHWVDKLAELGEPAPDPATHRRGAKARLLIWLAGRFGASSV
                     LPLVERAEIADAGHYDGVPDATVAMATDERIHARVLAGLTPATAKSGILRGERWHRGD
                     ASGAMRAAIFGVNDGLVSNLSLVMGVTGGEASRHFIVLAGLAGLLAGAFSMGAGEFIS
                     VTSQRELFQREIALESEEIEAMPDEEANELALIYRAKGIGKEEAEAMAARIMRDHASA
                     LDTMAREELGLNPDELGSPTKVAASSFASFASGALFPVLPWLFSAGTAAFVSSVLLSA
                     VVLFVVGAGISLLTGRPLLRVGVRQLLVGGAAAAATFGIGRLIGVSVN"
     gene            7382..>7518
                     /locus_tag="VF995_02130"
     CDS             7382..>7518
                     /locus_tag="VF995_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEX9342400.1"
                     /translation="MAADELQRILLVGPPGAGKGSLAPRLSELLGVPHVSSGQLLRAS
                     VA"
BASE COUNT          988 a         2769 c         2753 g         1008 t
ORIGIN      
        1 cggggccgca tcatcacccc gctgaaggac cgcttcggcg ccgaggtccg cacccactac
       61 ccgctgcggc tcgacgacga gatccgcctg gtccgccagg aggcgatgct cacctggccg
      121 cggagccacc tcggcgagcc ggtcgtgccc gaccacatcc tggagatcat cgcccgcttc
      181 acccgcgcgg tccgcgaggc accccagatc gacgcccgct cgggcgtgtc ggcgcgtttc
      241 gccatcgccg ccaccgagac cgccgcggcc tcggcggtgc gccgcgcggt cctggccggc
      301 gagccggtcc cggtggtgcg cgtggtggac ctgcccgccg ccgtcccggc cgccctcggc
      361 aaggtcgagt tcgacgagct ggaggagggc cgcgagctgg agctgctgca ccacctgctg
      421 aagcgggcgc tcgccgagac ctaccgggcg cggctggccg gcgcggacgt ctccgggctc
      481 ctgcgccgct tcgacgacgg cgccaccgtc gagaccggcg agctgctcag cgccgccgag
      541 ctgctgcagc gcatcggccc ggtgccgggc ctggcgcagc tgctgcgccg cctgggcgac
      601 ggcgacgccg agacgccggg tctcgccgcc gccgcgctcg agttcgccat ggaaggcctc
      661 cacctcgcca aacgcctcgg caaggaagag ctccctggcc gcgtcgtcta cggcaggtaa
      721 ccatgagcgg cgcctatcgc tacggcccct ggcgcggcgg ccccgatccg ctggagccgc
      781 cgttcgatct cggcgaggcc atggaccggc tgggggagcg ggtgctggcc ggccagcggc
      841 ccggagaggc cctcaacgac ctgctccgca aggggatgcc ggggctgcgc ggcctgcgcg
      901 acctgctacg gcaggcgcgg gaccgccgtc gcgagctgct gcgcgagcgg ggccgcctcg
      961 gcggcgtgct ggaccaggtc cgcgagctgc tcgaccgcgc cctcgaccag gagcgccagg
     1021 cgctgttccc ggatccctcc gacgccgcgc ggctggccga ggcggagctg gaccagctcc
     1081 ccgccgacac cgcccgcgcg gtccgccagc tcgccgacta ccagtggcgc tcgcccgaag
     1141 ccgccgccac ctacgaggag atccgcgacc tgctgcgccg cgaggtgctc gacacccgct
     1201 tccgcggcat gcgcgacgcg ctgcgcggcg ccacctccga gcagctccag cgcgtccgcg
     1261 acatgctcgg cgagctgaac gccatgctgg aggccgacgc ccgcggcgag gacaccagcg
     1321 agcagttcca gcgcttcatg cagcggtacg gcgacttctt ccccgagaac ccgcagaccc
     1381 tcgacgagct gatcgacgtg ctggcacgcc gctcggcggc ggcgagccgc ctgctggcgt
     1441 cgctgtcgcc ggagcagcgc gaggagctgg ccggactgat gcaggccgcc atgggcgatc
     1501 tcgggctgga gtacgagctg agccgcctcg gccaggcgct ccgctcccgc cgcccggacc
     1561 ttcgctgggg cgagcgcgcc ccgatcggcg gccagcagcc gctcggcctc gccgacgcca
     1621 ccaccgcgct ggaggagctg gccgacctgg aggcgctgga ggactccctc gcccaggact
     1681 acccgggcgc cagcctcgac gacgtcgacc tggacgcggt ccagcgcgcc ctcggcgagc
     1741 gcgcgaccgc cgacgtcgag gcgctgcggg cgctggagcg ggagctgacc gagcagggct
     1801 acctgacccg cgaccacggc cgcctgcgcc tcacccccaa ggcgatccgc cgcatcggcg
     1861 ccgccgcgct gcagcgcatc ttccgcaccc tggaggcccg cgaccggggc gaccacgaga
     1921 tcgccgacgc gggcctcgcc ggcgagccga ccggcagctc ccgcccatgg aacttcggcg
     1981 atgagcagcc gctcgacgtg gtccggaccg tcaagaacgc ggtcctgcgg cagggcccgg
     2041 cgaccgcgcc ggggcgcccg cgcgtgaccc tcacccccga cgacttcgag gtgctcgaca
     2101 ccgaacggcg ctcctcggcg gcggtgtgcc tgctgatcga cctgtcctac tcgatggtgc
     2161 ttcgcgacgc ctggggggcg gccaagacca ccgcgctggc gctgcacagc cttagtacca
     2221 gcaggtttcc ccaggacgag ctgaccgtca tcggtttctc ggcgatggcg cggaccctgc
     2281 cggcggggga gctggccgaa cttgagcccg acatggtgca gggcaccaac ctgcagcacg
     2341 cgctgctgct cgcgcgccgc tggctggcac gccgtcccga ggcggagccg gtggtgctgc
     2401 tgatcaccga tggtgagccc accgcccacc tggaacctga cggggtgcca tggttccagt
     2461 ggccgccgct gccccgcacc ctggagctga ccctggccga ggtgctgcgc ctcaccaagc
     2521 agggcatcac catcaacgtg ttcatgctcg acagcgagcc gcgcctggtc cgcttcgtgc
     2581 aggcgctcgc gcggctcaac cacggccgtg tattctcacc cgcgaccgac cggctcggcg
     2641 actacgtggt cagcgactac ctgcgggccc gccgtggccg ccgccgctcc gcgtgaacgt
     2701 cttgcgttga catcacggca gcatcaaatt acagtgatgt aagttcccgt ccggctctgg
     2761 ccggcgggat cgggaagggg ccccgggccg cgaggggaag cgtggtcagg ggcccctttc
     2821 ccatgtccgc atgtccgcca gtctggaggt gccggggcca tgcccgaggt gatgccgagc
     2881 gccacaacgg gcgccgtcga cctgccgctg cgccggcttg acccggagct gccgctgccg
     2941 gcctacgccc atccgggcga cgccggcgcg gacctccgcg cggccaccgg cgtgaccctg
     3001 ccgccgggcg gccgggcggt ggtcggcacc ggcgtcgccg tcgccgtgcc cgagggctac
     3061 gccgccttcg tccatccccg ctccgggctc gccgcccgca acggcatcac cgtggtcaac
     3121 gccccgggca ccgtcgacgc cggctaccgc ggcgaggtca aggtgatcct gctcaacacc
     3181 gacccagccg agccgtgcac gatcgagcga ggcgaccgca tcgcccagct ggtcgtccag
     3241 ccggtcgtcc aggcccgctt cctggaggtc gccgagctgc ccgccacacc gcgcggcgac
     3301 ggcggcttcg gctccaccgg ccggtaggca ccgccgctgg gcctcgctgg tcgtatcctg
     3361 ggctcaggat tgcccgtctc gggcagtggg aagtcaggaa gcggcatgga ccagaaccag
     3421 cgcgaccagc aacagcgccc ctggggcgag cggctcgccg acgagggcgc tgccgcccgc
     3481 gacgagcatt ccgtcaagcg cctgctctct gacgccatcg gcgtcgtccg cgagcgtctc
     3541 ggcgaggtca gccgcaacgg cgtcgggccg ctgctggagg aggccgccgg ccaggtccgc
     3601 ccggtcaccc gggaggagct gcggcgccgc tacccgggcc tcgacgacga cgaggtcgcc
     3661 cgccggctga tcaaccgggc cgcccgcacc gccgccgggg tcgcgctcgg catcggcggc
     3721 atgctcgccg cgcaggaggc gctggcagtg gtctccgccg gcggcaccct cggcaccctc
     3781 ggcgtcagcg cgctcgccga ggtgctggtg ctgttcattg tggaggccaa gcttcgcgcc
     3841 gacctcggcg cgctcgccgg ccagcccgcc cgcaccccaa aggagctggt cggcgcggtg
     3901 ctgggcgagg tcgccgcagc cggtggctgg cgcagcctgc ggggccggtc gctgcggcgg
     3961 gccttccccg agatggcggc ccggcgggtc gccgcccgcg tcgtcgcgct ggtgccgcgc
     4021 cgcttcgccc gcatcatcgc cccggagatc gtcgccccac tggtgggtgc ggccttcgcc
     4081 gcgcggatgg cggcccgcca ggtccgcgcc gccggggagc agtgctgggc ggcgctgccc
     4141 cgcaccaccg ccacctgggg cacccccatc ccctccagcg gcaacggctc ggcgccgccg
     4201 cgctcctgag gccagacgcg cgacagcccc ggtggtggga agccggggct gtgcggtgga
     4261 acctgcggtc tagctgcggt ctagcgctcg atcggcacgc cgaccaggga gccccactcg
     4321 gtccaggaac cgtcgtagtt gcggacgttg tggtagccga gcagctcgtg gagcacgaac
     4381 caggtgtgcg ccgagcgctc gccgatgcgg cagtaggcga ccacgtcctt gtcggcggtc
     4441 acgcccgcgc cgccgtagag gccgcgcagg tcgtcgtcgg acaggaactt gccggtggtg
     4501 ccgtcgacgg ccttgagcca ggggatgttg gcggcgccgg ggatgtggcc ggggcgctgc
     4561 gccgactcct ggggcaggtg gtcgggggcg agcttctcgc cgcggtactc ctccgggctg
     4621 cggacgtcca ccagcgcggt gccggcgccg ttgacatagc gggccagcac ctcgtcgcgg
     4681 aacgcgcgga tggtgaagtc cggctggcgc accgggtagc cggaggtggc gctgatcgcc
     4741 ggcggctcgc tggtcagctc gcggccctcc agctcccagg ccttgcggcc gccgtcgacc
     4801 agcttgaccg ggccgtggcc gtagaccttg tagtaccagt aggcgtaggc ggcgaaccag
     4861 ttgttgttgc cgccgtagag caccaccagg gtgtcggggg tgatgccgag cgagtcctgc
     4921 agcttggcga aggcctccgg cgggatgaag tcgcgccgta gcgggtcctg caggtcggtg
     4981 ttccagttga cggcgaccgc gccggggaca tggccgcggc cgtaggcgtc ggtgtcctcg
     5041 tcgacctcaa cgatccgggt gttggggtcg ttgagatgct cggcgagcca gtcagttgtg
     5101 accagggcgt cgtctttggc catgattgga ctccttggtg ctgagacgag atgggaacgc
     5161 ggacagcgag aaggcgagca gggaaacagg aagcggaggg gtccaggccg cggctcggct
     5221 cagcgtcagc gggtgagcgg cgccgcgacc cggcgacacg gcggacggcc agcggccgag
     5281 ttcccggtgc cgaggcccat cggggcgcac gcgcgcgtcg tgagggccgc cggccccgac
     5341 cttcggcggg ccgtcgtggt cggcagcatc agtgcaatca tgtggtgcta caggctgcca
     5401 gctcagccgg cgttacgtca acgtgccggt tgtgaggcaa gcacgggaat cgaatgaatc
     5461 cagcggatcg ggagcggtga tgacgatggc gttcgttcca gacagagatg acgccctcgt
     5521 ggtggtcgac gtgcagcgtg acttctgccc gggcgggagc ctcgcggtgc ccgagggcga
     5581 ccaggtggtg cccctgctca accggctggc gccccgcttc gccacggtgg tgaccaccca
     5641 cgacacccat ccgcccgacc actcctcgtt catcgcgcag ggcggcccct ggccgccgca
     5701 ctgcgtggag ggcacgctcg ggtgggagcg gcatccggac ctgcgggtgg aggccgacta
     5761 ccaggtcctg aagggccgcg agcgggacca ggacggctat accggctgga accaggagct
     5821 ggcggacttc ctgaccaaac gcggtgtccg ccgggtggtg gtcggcgggc tcgccctcga
     5881 ctactgcgtg aaggcgaccg ccctcgatgc ccgcgccgcc ggccatgacg tggtcctgct
     5941 caccgatgcg acccgggccg tgaatctcca gcccggcgac ggcgagcagg ccctgcacgc
     6001 cctgcgcgcc gccggggtcc gcgaggaccg ggctgagggc tgagggccgg ctctgacggc
     6061 tctgaccgct ctggatgacc cgctgactgg cgcagcggcg agcggccgtc caggccgctt
     6121 tgttgcgcag ttcggagggc atggccaaca cttgacggcg cacatggcga tcagcggcca
     6181 gcgagcggtg agaggaggca agggtttggc gtccaaagcc aaggcgacgt ccgagccgga
     6241 cgcgaagtcc agagcgcggg ggttccgcga gcactgggcc gacgagatgg acggcgcggc
     6301 catgtaccgt gccctcgcca gcgccaccgg ggacggggag cagcgggaga tcttcctgga
     6361 gctgtccaag gccgaggagc gccacgcccg ccactgggtc gacaagctgg ccgagctggg
     6421 cgagcccgcc ccagacccgg cgacccaccg gcgcggcgcc aaggcgcggc tgctcatctg
     6481 gctggctggg cgcttcggcg ccagctcggt cctgccgctg gtcgagcggg ccgagatcgc
     6541 cgacgccggc cactacgacg gcgtccccga cgcgaccgtg gcgatggcca ccgacgagcg
     6601 catccacgcc cgcgtgctcg ccggcctgac gcctgcgacc gcgaaatcgg gcatcctgcg
     6661 cggcgagcgc tggcaccgcg gcgacgcctc cggcgcgatg cgggcggcga tcttcggcgt
     6721 gaacgacggc ctggtctcca acctgtcgct ggtcatgggg gtcaccggcg gcgaggccag
     6781 ccggcatttc atcgtgctcg ccgggctcgc cgggctgctc gccggcgcct tctcgatggg
     6841 tgcgggcgag ttcatctcgg tcacctccca gcgggagctg ttccagcgcg agatcgccct
     6901 cgaatcggaa gagatcgagg ccatgccgga cgaggaggcg aacgagctgg ccctcatcta
     6961 ccgcgccaaa ggcatcggca aagaagaagc cgaggcgatg gccgcccgga tcatgcgcga
     7021 ccacgccagc gccctcgaca ccatggccag ggaggagctt ggcctcaacc ctgacgagct
     7081 gggctcgccg acgaaagtgg cggcctccag cttcgcctcc ttcgccagcg gcgcgctgtt
     7141 cccggtgctg ccgtggctgt tctccgccgg gaccgccgcg ttcgtctcgt cggtgctgct
     7201 ctccgccgtg gtcctgttcg tggtcggggc tggcatcagc ctgctcaccg gccgcccgct
     7261 gctgcgcgtc ggcgtccgcc agctgctggt cggtggggcc gccgccgcgg caaccttcgg
     7321 aatcgggcgc ctcatcgggg tgagtgtcaa ttgagcgcga gtgatgacag cgatggcagc
     7381 gatggcagct gacgagctcc aacggatcct cctggtcggc ccgccaggcg cgggcaaggg
     7441 cagcctcgcc ccgcggctct ccgagctgct cggggtgccg cacgtctcct ccggccagct
     7501 gctgcgcgcc tcggtggc
//
LOCUS       DASWDC010000065         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_591517, whole genome shotgun sequence.
ACCESSION   DASWDC010000065 DASWDC010000000
VERSION     DASWDC010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591517"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VF995_02135"
     CDS             complement(<1..368)
                     /locus_tag="VF995_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX9342401.1"
                     /translation="MALTVVDHPLAGTMLTALRDAATPPPLFRLLAKRLSLTLAIEAT
                     RTVPLRPREVTTPLAEAAGTAFAAPLVAVPILRAGLGMLEAVTELFPEVAVGYLGLER
                     DHATFQPSAYYTKLPPLRDS"
     gene            complement(417..1010)
                     /locus_tag="VF995_02140"
     CDS             complement(417..1010)
                     /locus_tag="VF995_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX9342402.1"
                     /translation="MRRVVIGILLAVLAYFAVTTLLVVVWMGRDQRPHADAIVVLGAA
                     QYNGRPSAIYEARLQHAVDLYSSGVARLVVFTGGKEPGDRFTEGGSGAHWAELHGVPA
                     SATVIEDQSRTTYQNLSGTKRLLQSRVRDRPMHIVVVSDPYHMYRAITQARGLGFKAY
                     ASPTRTSPVSASRLKLTEAVLREDIALGGYLLTRGHA"
     gene            1165..2256
                     /locus_tag="VF995_02145"
     CDS             1165..2256
                     /locus_tag="VF995_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9342403.1"
                     /translation="MRRTYRAVALLALVALVAAGCGKKPASTSSGGGGTAAKYPNFLA
                     CEVTDTGGVDDRSFNATSWKGVQNAQKDFGVQIKLLESSTANDYVPNLRAFAQQKCGL
                     IVTVGFLLGDATRDSAKANASEKYAIVDFAYDPKDVPNNNVLGLTFSTDQAAFLAGYV
                     ASAMSKTHKLGTFGGINIPTVTIFMNGFWAGMKYYNKKNNANTQLLGWDPAKPKGGLF
                     TGDFTNQDNGKRLAETLMSQGADIILPVAGNVGLGAAAAVKAAGGDKHMIWVDTDGCV
                     SAAQYCSLFITSVQKHMDVAVEDAVKTTASGAYKGGTYVGTLANNGVGIAPFHDFDSQ
                     VPANVKADLDTIKQGIISGTISVKTSDYS"
     gene            2298..3851
                     /locus_tag="VF995_02150"
     CDS             2298..3851
                     /locus_tag="VF995_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342404.1"
                     /translation="MRLELKGITKRFPGVVANDGIDLTVEEGEIHALLGENGAGKTTL
                     MNVLYGLYHADEGEILLDGEPRRLASPRDAIDAGIGMVHQHFMLVPVFTVAENVMLGM
                     EPTSRFGLLNRARARHQVRALSQRYGLAVDPDALVEDLPVGVQQRVEIVKALLREAKV
                     LILDEPTAVLIPQETEELFEVMRSLKASGHSILFITHKLKEVLPVADVITVLRGGRVV
                     GSTVPGETSEQELASMMVGRTVLLRVAKEPANPQEVVLDVDGLTVLDDRGQVWVDQLS
                     LRVRSGEILAIAGVQGNGQTELVEALTGLRPVAGGTVRLDGNSLTGVHPRGVLRAGVG
                     HVPEDRVKNGLVATFSVAENLVLDTYDTAPFAHGLVMDLDTVDRTAGERAREFDIRTP
                     SIRTAVGTLSGGNQQKVIIARELSRPIKLLIASQPTRGLDVGSIEYVHRRIVEARDQG
                     AAVLIVSSELDEVLALADRIAVMCGGRIVGERPAGADRDEIGLLMAGAGTAGQGGGQT
                     DGEADAEAR"
BASE COUNT          640 a         1378 c         1381 g          536 t
ORIGIN      
        1 tagctgtcgc ggagcggcgg gagcttggtg tagtaggccg acggctggaa ggtggcgtgg
       61 tcgcgttcca ggcccaggta gccgactgcc acctcgggga acagctcggt cacggcctcc
      121 agcatgccca ggcccgcgcg caggatcggg accgcgacca gcggcgcggc gaaggcggtc
      181 ccggcggcct cggccagcgg agtggtcacc tcgcggggcc gcagtggcac ggtccgggtc
      241 gcctcgattg ccagggtcag cgacagccgt ttggccagca gccgaaacag cggcggcggg
      301 gtggcggcgt cgcgcagcgc ggtcagcatc gtgcccgcca gcgggtggtc gacgacggtc
      361 agcgccaccg cgggcctcct ttctcagtgc ctgctcgggc ttgacagggc caggtgtcag
      421 gcgtgtccgc gggtgaggag gtagccgccg agggcgatgt cctcgcgcag caccgcctcg
      481 gtcagcttca gccggctggc gctcaccggg ctggtgcggg tgggggaggc gtaggccttg
      541 aagcccagcc cgcgagcctg ggtgatcgcc cggtacatgt ggtaggggtc ggagacgacc
      601 acgatgtgca tcggccggtc ccgcacgcgg ctctgcagca gccgcttggt ccccgacagg
      661 ttctggtagg tggtgcggct ctggtcctcg atcaccgtgg cgctcgccgg gaccccgtgc
      721 agctccgccc agtgggcccc ggagccgccc tcggtaaagc ggtcgcccgg ctccttgccg
      781 ccggtgaaca ccaccagccg cgccacaccg ctgctgtaga ggtccacggc gtgctgcaac
      841 ctggcctcgt agatcgcgga ggggcggccg ttgtactgcg cggcccccag caccacgatg
      901 gcgtcggcgt ggggccgctg gtcgcgtccc atccagacga cgaccagcag cgtcgtgacg
      961 gcgaaatacg ccagcacggc cagcaggatc ccgatgacga cacggcgcat ccccagattc
     1021 tagaagggtg gggtgccaga cgccctgtcc gcggccctct ggcgacggat cgtgaagggg
     1081 ttgtgcacag gtcgtgacca gccctggtcc caaaggctag actccgcttc agatggtggg
     1141 aaggtgggat tcggaggagg cgccatgaga aggacctacc gggcggtcgc tctgctcgcc
     1201 cttgtcgcgc tggtggcggc gggctgcggc aagaagcccg cgagcacgag ttccggcggt
     1261 gggggcacgg ccgccaagta cccgaacttc ctcgcctgcg aggtgaccga caccggcggc
     1321 gtggacgacc ggtcgttcaa cgcgacctca tggaaaggcg tgcagaacgc ccagaaggac
     1381 ttcggcgtcc agatcaagct gctcgaatcc agcaccgcca acgactacgt gcccaacctg
     1441 cgggccttcg cccagcagaa gtgtggcctg atcgtcaccg tcggcttcct gctcggcgac
     1501 gccacccgcg acagcgccaa ggccaacgcc agtgagaagt acgccatcgt ggacttcgcc
     1561 tacgacccga aggacgtgcc caacaacaac gtcctggggc tgaccttctc gaccgaccag
     1621 gccgcgttcc tcgccggcta cgtcgcctcg gcgatgtcca agacccacaa gctcggcacc
     1681 ttcggcggga tcaacatccc cacggtgacg atcttcatga acggcttctg ggccggcatg
     1741 aagtactaca acaagaagaa caacgccaac acgcagctgc tcggctggga cccggccaag
     1801 cccaagggcg gcctgttcac cggcgacttc accaaccagg acaacggcaa gcggctggcc
     1861 gagacgctga tgagccaggg cgccgacatc atcctgccgg tggccggcaa cgtcggcctc
     1921 ggcgccgccg ccgcggtcaa ggcggctggc ggcgacaagc acatgatctg ggtcgacacc
     1981 gacggctgcg tcagcgccgc gcagtactgc agcctgttca tcaccagcgt ccagaagcac
     2041 atggacgtcg cggtcgagga cgccgtcaag acgaccgcca gcggcgccta caagggcggc
     2101 acctatgtcg gcaccctggc caacaacggg gtcggcatcg cgccattcca cgacttcgac
     2161 agccaggtgc ccgccaacgt caaggctgat ctggacacca tcaagcaggg catcatcagc
     2221 gggacgatct cggtgaagac ctccgactac agttagccgc cggcgaccgg cagtcggctg
     2281 cgaggcaggg ggacgccctg agactcgagc tgaagggcat caccaagcgg tttcccgggg
     2341 tggtcgccaa cgacggcatc gacctcaccg ttgaggaggg cgagatccac gccctcctcg
     2401 gcgagaacgg cgccggcaag accaccctga tgaacgtcct ctacgggctc taccacgccg
     2461 acgagggcga gatcctgctc gacggggaac cgcggcggct ggccagcccg cgggacgcga
     2521 tcgacgccgg gatcggcatg gtgcaccagc acttcatgct ggtcccggtg ttcaccgtgg
     2581 ccgagaacgt gatgctcggc atggagccca ccagccgctt cggcctgctc aaccgcgcca
     2641 gggcacgcca ccaggtgcgc gcgctgtcgc agcgctacgg gctcgcggtc gaccccgacg
     2701 ccctggtcga ggacctgccg gtcggggtgc agcagcgcgt cgagatcgtc aaggcgctgc
     2761 tgcgcgaggc caaggtgctg atcctcgacg agccgaccgc tgtgctcatc ccccaggaga
     2821 ccgaggagct gttcgaggtg atgcgctccc tcaaggcctc gggtcactcg atcctgttca
     2881 tcacccacaa gctcaaagag gtcctgccgg tcgccgacgt catcaccgtg ctgcggggcg
     2941 gccgggtggt cggctcgacc gtgcccggcg agaccagcga gcaggagctg gcctcgatga
     3001 tggtcggccg caccgtgctg ctgcgggtcg ccaaggagcc ggccaacccc caggaggtgg
     3061 tgctcgacgt cgacgggctc accgtgctcg acgaccgcgg ccaggtctgg gtcgaccagc
     3121 tctccctgcg ggtccgctcg ggcgagatcc tggcgatcgc gggggtgcag ggcaacggcc
     3181 agaccgaact ggtcgaggcg ctcaccggcc tgcggccggt ggccggtggc acggtccggc
     3241 tcgacgggaa ctcgctgacc ggcgtccacc cgcgcggcgt gctgcgcgcc ggggtcggcc
     3301 acgtacccga ggaccgcgtc aagaacggcc tggtcgccac cttctcggtg gccgagaacc
     3361 tggtgctgga cacctacgac accgcgccgt tcgcgcacgg cctggtgatg gacctggaca
     3421 ccgtcgaccg caccgcgggc gagcgggccc gggagttcga catccggacg ccctccatcc
     3481 ggacagcggt cgggacgctg tccggcggca accagcagaa ggtgatcatc gcgcgggagc
     3541 tgtcccggcc gatcaagctg ctgatcgcct cgcagccgac ccgcggcctg gacgtcggct
     3601 cgatcgaata cgtccaccgc cgcatcgtcg aggcgcgcga ccagggcgcc gccgtgctga
     3661 tcgtctcctc cgagctcgac gaggtgctgg ccctggccga ccggatcgcg gtgatgtgcg
     3721 gcggccggat cgtcggcgag cgccccgccg gcgccgaccg cgacgagatc ggcctgctca
     3781 tggccggcgc cggaaccgcc gggcagggcg gcggccagac cgacggggaa gctgacgcgg
     3841 aggcccgatg accgagccga tcaccaaggc acccgcgccc gagcagccgt ccgcgccgcc
     3901 gcagccgccc agcttcgccg cgcgcctcct gcggg
//
LOCUS       DASWDC010000066         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_174264, whole genome shotgun sequence.
ACCESSION   DASWDC010000066 DASWDC010000000
VERSION     DASWDC010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174264"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VF995_02155"
     CDS             <1..202
                     /locus_tag="VF995_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9342405.1"
                     /translation="LDAHGHLTSREQEVVELVRAGHNSRQIAERLVVSERTVESHRWN
                     AYQKLGVHNARELHAIFDAETR"
     gene            complement(251..958)
                     /locus_tag="VF995_02160"
     CDS             complement(251..958)
                     /locus_tag="VF995_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9342406.1"
                     /translation="MTIRVLIADDQMLLRAGFRMIIQTQQDMEVVGEAADGVEAVALA
                     RRLLPQVVLMDIRMPNLDGIQATSRITAPGVAVGAGPGTAPKVVILTTYDLDEYVFDA
                     LASGASGFLLKDIPPEDLVRAVRVAAQGEALLAPSVTRRLVEQFVRQRPPTPLAAHRL
                     DPLTEREAEVLRLVARGLSNTEIATALCLGESTVKTHVGHILDKLQLRDRVQAVILAY
                     EAGLVRPGQPEPEPEDA"
     gene            complement(955..2271)
                     /locus_tag="VF995_02165"
     CDS             complement(955..2271)
                     /locus_tag="VF995_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9342407.1"
                     /translation="MSQAAAPEPEPEPPTRAGFWVAGRFTPGQVDWMVVGAAALLAVP
                     TLPHALAGQKPVAVDLAALPFGTVPLLWRRRRPGLVLAVIAAAFAVSAVLGSGTPDPG
                     GVLFGVGAAALYGDRRVRLVAGAVALGALVPTFALMLLSGSFEGRPLGHLAATVSGSA
                     VAWVAGDRTRTRRAYLAQLEERAARLEREREDQARRSAQAERNRIARELHDVVAHNVS
                     VIAVQAGAARMASGADPEQAAQALGVIERTARSTLTELRALLGMLRRQDGERLDASRG
                     PQPTLAQLDALAAQARQAGLRLQTRVRGDLGGLPATVDLSAYRIVQEALTNTLKHAPG
                     ARVHLLVRRTSRALDVVVVDDGPGAPTGGPGPGSESRAGSGAGGQGLIGMRERAALVG
                     GQLRAGPALGGGFRVEAHLPVEAEPVEAEAAEAAEAEAADAGVTLP"
     gene            2454..3173
                     /locus_tag="VF995_02170"
     CDS             2454..3173
                     /locus_tag="VF995_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342408.1"
                     /translation="MAMIEVQGLTKRFGEVLAVDQLSFEVDRGTVVGFLGPNGAGKTT
                     TLRALLGLVRPSDGAATFGGKAYAELAEPLRHVGAVLEASSFYPGRSARNHLRVQATA
                     AGIDPARVEAVLDLVGLQGAARRQVGGFSLGMRQRLGIAAAMLGDPEVLILDEPANGL
                     DPEGVRWLRGLVRGLAEEGRTVLVSSHILAEVAQTVDSVVIIDKGRLVVQSTLRELTA
                     GANLEDVFFQLTAASSDKEGS"
     gene            3173..3934
                     /locus_tag="VF995_02175"
     CDS             3173..3934
                     /locus_tag="VF995_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342409.1"
                     /translation="MTRAVRTELLKLRTIRLPLGLLGTAAGLTALITTLGAARAGGSG
                     HMAPPPLYTAAGQQDLFIRTTFGMLLAAVFGVTVASGEFRHGTATATYLATPNRTRVL
                     IAKAVVAAGAGLLFGLVATALTTGITLAFVAAKGYHVALAGGTIARFAAGAILGSGLL
                     AAAGAGVGSLLRGQLSAIITVFVWGLVLEPLVGGLFDAVQPYLPVTTATTMAGSALGG
                     GVSPLPFAVAAALVAGVAVLLAVVAGWTRVPNDVS"
BASE COUNT          502 a         1427 c         1492 g          514 t
ORIGIN      
        1 cctcgacgcc catggccacc tgaccagccg ggagcaggag gtcgtcgagc tggtgcgcgc
       61 gggccacaac agccgccaga tcgccgagcg gctggtggtc tcggagcgaa ccgtggagag
      121 ccaccgctgg aacgcctacc agaagctcgg cgtccacaac gcccgcgagc tgcacgcgat
      181 cttcgacgcg gagacacgct gacagctgac agtggggggg ctgccggggg actggggttc
      241 gagccgggac tcaggcgtcc tccggctccg gctccggctg gcccgggcgg accaacccgg
      301 cttcgtaggc gaggatcacg gcctggaccc ggtcgcggag ctggagcttg tcgaggatgt
      361 ggccgacgtg ggtcttcaca gtgctctcgc cgaggcagag ggccgtggcg atctcggtgt
      421 tcgacaaccc ccgcgcgacc agccgcagga cctcggcctc gcgctcggtg agcgggtcga
      481 gccggtgggc cgccagcggc gtggggggcc gctggcgcac gaactgctcc accagccggc
      541 gggtcaccga gggggccagc agcgcctcgc cctgggcggc gacccggacc gcccggacca
      601 ggtcctcggg cgggatgtcc ttcagcagga agccgctggc accagatgcc agcgcgtcga
      661 agacatattc gtcgaggtcg taggtggtca ggatcaccac cttcggtgct gtccccggcc
      721 ccgctcccac cgccacccct ggggcggtga tccggctggt ggcctggatg ccgtccaggt
      781 tcggcatgcg gatgtccatc agcacgacct gcgggaggag ccggcgggcc agcgcgacgg
      841 cctccacccc gtcggcggcc tcgccgacga cctccatgtc ctgctgggtc tggatgatca
      901 tgcggaaccc cgcccgcagc agcatctggt cgtcggcgat cagcacccgg atggtcacgg
      961 cagcgtgacc ccggcgtccg ccgcctcggc ctcggctgcc tcggctgcct cggcctcgac
     1021 cggctcggcc tcgaccggga ggtgggcctc gacgcggaag ccgcccccga gcgcggggcc
     1081 ggcgcgcagc tgcccgccga ccagggcggc gcgctcccgc atcccgatca ggccctgccc
     1141 tccagccccc gatccagccc tcgattcgga tcccggcccg ggcccaccgg tcggggcgcc
     1201 ggggccgtcg tcgaccacca ctacgtcgag tgcccggctg gtgcggcgga ctagcaggtg
     1261 gacacgcgcg ccaggggcat gcttgagggt gttggtgagc gcctcctgga cgatccggta
     1321 ggccgacagg tccaccgtgg ccgggagtcc cccgaggtct ccccgcaccc gcgtctggag
     1381 gcggagccct gcctggcgtg cctgggcggc cagggcgtcg agctgggcca gggtgggctg
     1441 cggcccgcgg ctggcgtcga gccgctcgcc gtcctgccgg cgcagcatcc cgagcagcgc
     1501 ccgcagctcg gtcagggtcg accgggcggt gcgctcgatc acgccgagcg cctgggccgc
     1561 ctgctcgggg tcggcgcccg aggccatccg ggcggcgccg gcctgcaccg cgatcacgct
     1621 cacgttgtgg gccaccacgt cgtgcagctc ccgggcgatg cggttgcgtt cggcctgggc
     1681 gctgcggcgg gcctggtcct cgcgttcccg ctccagcctg gccgcccgtt cctcgagctg
     1741 ggcgaggtag gcgcggcggg tccgggtccg gtcgccggcc acccacgcca ccgcggaccc
     1801 ggacaccgtc gccgccaggt gccccagcgg cctgccctcg aaggacccgg acagcagcat
     1861 caaggcgaac gtgggcacca gcgcccccag cgcgacggca ccggcgacga gccggacgcg
     1921 gcgatccccg tacagcgccg cggctcccac accgaacagc acccctcccg ggtcgggcgt
     1981 cccgctcccg aggaccgccg agacggcgaa cgcggcggcg atcacggcga gcaccagccc
     2041 gggccggcgc cgccgccaca gcagcggcac ggtcccgaac gggagggcgg cgaggtcgac
     2101 ggccaccggc ttctggccag ccagtgcgtg cggcagggtc gggaccgcca gcagcgcggc
     2161 ggcgccgacc accatccagt cgacctggcc cggagtgaac cggccggcga cccagaaccc
     2221 cgcgcgggtc gggggttcgg gttcgggttc gggtgccgcg gcctggctca tggcttcgac
     2281 cataacccca ggccaccgcc tgcgcatccg cctgggaggg gagcggccgg cgaccggaat
     2341 ccacctgtgg gaggaggagg aaccacctcg gcggcgatgg cggcggctcc ctgtggccct
     2401 agcgtcggct ctggcagcgc aaacgggacc agcggcacgg caaaggagac gccatggcaa
     2461 tgatcgaggt acaggggctg accaaacggt tcggcgaggt gctcgccgtc gaccagctga
     2521 gcttcgaggt ggaccgcggc accgtggtgg ggtttctcgg gcccaacggc gccgggaaga
     2581 cgaccacgct ccgcgccctg ctcggcctgg tgcggcccag cgacggtgcg gccaccttcg
     2641 gcgggaaggc ctacgcggag ctggctgagc cgctgcggca tgtgggcgcg gtcttggagg
     2701 cgtcgagctt ctatcccggc cggagcgcgc gcaaccacct gcgggtgcag gcgacggcgg
     2761 ccgggatcga cccggcccgg gtcgaggcgg tgctcgacct ggtcggactc cagggcgccg
     2821 cgaggcgaca ggtcggcggg ttctcccttg ggatgcgcca gcgcctcggg atcgccgcgg
     2881 cgatgctcgg cgatcccgag gtgctgatcc tggacgagcc cgccaacggg ctcgaccccg
     2941 agggggtccg gtggctccgc ggcctggtcc gggggctcgc cgaggagggg cggacggtgc
     3001 tggtgtcgag ccacatcctg gccgaggtcg cccagacggt ggacagcgtg gtgatcatcg
     3061 acaaaggtcg cctggtggtc cagtcgacgc tcagggagct gaccgcgggc gccaacctgg
     3121 aggacgtgtt cttccagctc accgccgcaa gcagcgacaa ggaggggtcc tgatgacccg
     3181 ggcggtccgg accgagctgc tgaagctacg caccattcgc ctgccgctcg gcctgctggg
     3241 cacggcggcg gggctgaccg cgctgatcac gacccttggc gcggccagag ccggcgggtc
     3301 cgggcacatg gcgccgccgc cgctgtacac cgcggccggc cagcaggacc tgttcatcag
     3361 gacgaccttc ggcatgctcc tggccgcggt gttcggggtc acggtcgcaa gcggcgagtt
     3421 ccgccacggg accgccacgg cgacctacct ggcgaccccg aaccggaccc gtgtcctgat
     3481 cgccaaggcc gtcgtcgcgg cgggggccgg gctgctgttc ggcctggtcg ccaccgccct
     3541 caccaccggg atcacgctgg cgttcgtggc cgccaagggc taccacgtgg cgctcgccgg
     3601 gggaaccatc gcccgcttcg cggccggcgc gatcctcggg tcggggctgc tcgccgcggc
     3661 tggggccggg gtggggtcgc tgctgcgcgg ccagctcagc gcgatcatca cggtgttcgt
     3721 gtgggggctc gtcctggagc cgctcgtcgg cgggctgttc gacgccgtcc agccctacct
     3781 tccggttacg acagcgacca cgatggccgg gtcggcgctc ggtggcggcg tcagcccctt
     3841 gcccttcgcc gtggcggcgg cgctggtcgc gggggtggcg gtgttgctgg cggtggtggc
     3901 cgggtggacc agggtgccga acgacgtctc gtagc
//
LOCUS       DASWDC010000067         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_925384, whole genome shotgun sequence.
ACCESSION   DASWDC010000067 DASWDC010000000
VERSION     DASWDC010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925384"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VF995_02180"
     CDS             complement(<1..648)
                     /locus_tag="VF995_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX9342410.1"
                     /translation="MLVLDGAAVRALFPMEAAIVEMRGALARYSAGEVVQPLRLVIQA
                     PDSPNLLALMPAWTGPTAGDGGGRGALGLKAVAIFPGNPGRGLDVHQGAVTMFDPDTG
                     VPAATMDAATVTEIRTAAVSGVATDALARPDAGDLAVLGAGVQARSHLEAMAAARPLR
                     RVRVWSRTAAHAQALADWAGTRFGPPVEVCATPAAALAGADLACTTLACVEPVVDA"
     gene            complement(635..2215)
                     /locus_tag="VF995_02185"
     CDS             complement(635..2215)
                     /locus_tag="VF995_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEX9342411.1"
                     /translation="MIRRPLRTRASGQRSGTGGPPWDPAIEPLIRSHRAAHPRADMAL
                     LARAYLVAEREHRGQMRQSGLPFITHPLEVTMLVAALGMDTTTLVASLLHDTVEDTGY
                     TLDLARAEFGDEVALLVDGVTKLDKLTFGAAAEAETIRKMLVAMGRDPRVLVIKLADR
                     VHNMRTLGAKSRPSQVRIARATLEVLAPLADRLGIQVFRRELEDLALAILHPPEYEQL
                     VRLTARRVPQRQRYLATVIEPLEADLRAARVRATVRDHPRHAFSVYETIIQRGGSITD
                     VYDNARVLIVIDGDASGCYTTLGMVHARWTPVSGRFKDFIALPKFNFYQSLHTSIVGP
                     LDQLIDVQIRTEAMQRTAEYGIAANPAAVAERADDLRWLTRLLDWQRFASDPAEFLEA
                     LSFDLVEEEVLVFLPDGEALSLPTDATPVDAAYALSTEIGNRCVGARVNGRLVPLSRP
                     LQDGDVVEVLTSTVRHHGPSREWLRFVKSPKAHVAIRQWMADTGQFPAIGSDTDADDG
                     PGSEGDTQIEEVGSDAGA"
     gene            complement(2398..>3601)
                     /locus_tag="VF995_02190"
     CDS             complement(2398..>3601)
                     /locus_tag="VF995_02190"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEX9342412.1"
                     /translation="DAALARRLAWELQDAPVLKLGWERFARYHAAAARLRRTDDRLHR
                     WPLALGVAAVLVALVQHAVRGRVGAGSVADLALHWTVAALPFLATVVLLAGHRFGPGR
                     RWPLLRAAAEAARRELYRYRTATGPYAPASVPADADSDADSHADSHADSDAGTAARAA
                     ERDATLAGRLGAIDAGLMQTEVAAGGVPSYSGPLPPPWEGTTAGDDGLSPLDPNGYLE
                     LRLAERLGACRARVAVAERRRRRLQLLILAAGIAGSVLAVARLELWVGLSTAALFAAV
                     ASLAHLQVEAELLADNRAAARLEAIRARWLALPTGQRDPRRFAGLVEQTEAAADPAHA
                     DWSQQLADAITAAQSLAMVAAPLARDVGREQDQGDRQGPGALPPAGGSGTKRSPAARL
                     AGLFGNGR"
BASE COUNT          450 a         1297 c         1412 g          442 t
ORIGIN      
        1 ggcgtcgacg accggttcga cgcaggccag ggtggtacag gccaggtcgg cgccggcgag
       61 cgccgcggcg ggggtggcgc agacctcgac cggcgggccg aaacgggtgc cggcccagtc
      121 ggcgagcgcc tgcgcgtggg cggcggtgcg gctccacacc cgcacgcggc gcagcgggcg
      181 ggccgccgcc atcgcctcca ggtggctgcg ggcctgcacc ccggcgccga ggaccgcgag
      241 atcgccggcg tccggccggg cgagcgcgtc ggtggcgacg ccggagacgg cggcggtgcg
      301 gatctcggtg acggtggcag cgtccatggt cgcggccggc acgccagtgt ccgggtcgaa
      361 catggtcacc gcaccctggt ggacgtccag gccgcgaccc gggttgccgg ggaagatcgc
      421 gaccgccttg aggccgaggg cgccccggcc tccaccatcg ccggcggtgg ggccggtcca
      481 ggcgggcatc agggcgagca ggttggggct gtcgggggcc tggatcacca ggcgcagcgg
      541 ctggaccacc tcaccggcgc tgtagcgggc gagcgcgccg cgcatctcca cgatggccgc
      601 ctccatcggg aacagcgccc gcacggcggc gccgtcaagc accagcatcg gatccgacct
      661 cctcgatctg ggtgtcgccc tcgctgcccg gcccgtcgtc cgcgtccgtg tcgctgccga
      721 tcgcggggaa ctggccggtg tcggccatcc actggcggat cgccacgtgc gccttggggc
      781 tcttcacgaa ccgcagccac tcccgcgacg ggccgtgatg ccgcaccgtg gaggtgagca
      841 cctcgaccac gtcgccgtcc tgcagcgggc gcgacagcgg gaccagccgg ccgttgacgc
      901 gcgcgccgac gcagcggttg ccgatctcgg tcgacagggc gtaggcggcg tccaccgggg
      961 tggcgtcggt gggcagcgac agcgcctcgc cgtccggcag gaacaccagc acctcctcct
     1021 cgaccaggtc gaagctgagc gcctccagga actcggccgg atcggaggcg aaccgctgcc
     1081 agtccagcag ccgggtaagc caccgcaggt cgtcggcgcg ctcggcgacc gccgccgggt
     1141 tggcggcgat gccgtactcg gcggtgcgct gcatcgcctc ggtgcggatc tgcacgtcga
     1201 tcagctggtc cagcggccca acgatcgagg tgtgcaggct ctggtagaag ttgaacttcg
     1261 gcagggcgat gaagtccttg aagcggcccg acaccggcgt ccagcgcgcg tggaccatgc
     1321 cgagggtggt gtagcagccg gaggcgtcgc cgtcgatcac gatcaggacg cgggcgttgt
     1381 cgtagacgtc ggtgatgctg ccgccgcgct ggatgatcgt ctcgtagacc gagaaggcgt
     1441 ggcgggggtg gtcgcggacc gtcgccctga cccgcgcggc acgcaggtcg gcctccagcg
     1501 gctcgatcac cgtggccagg tagcgctggc gctgggggac ccgccgggcg gtcagccgca
     1561 ccagctgctc gtactcgggc gggtgcagga tcgcgagcgc caggtcctcc agctcgcggc
     1621 ggaagacctg gatgccgagc cggtcggcga gcggcgccag cacctccagg gtggcgcggg
     1681 cgatgcgcac ctggctcggc cgtgacttgg cgccgagggt gcgcatgttg tggacccggt
     1741 cggccagctt gatcaccagc acgcgcgggt cgcggcccat cgccaccagc atcttgcgga
     1801 tcgtctcggc ctcggcggcg gcgccgaagg tcagcttgtc cagcttggtg acgccgtcga
     1861 ccagcagcgc cacctcgtcg ccgaactcgg cgcgggccag gtcgagggtg tagccggtgt
     1921 cctcgacggt gtcgtgcagc agcgaggcca ccagcgtggt ggtgtccatc ccgagggcgg
     1981 ccaccagcat ggtcacctcc agcgggtggg tgatgaacgg caggccgctc tgccgcatct
     2041 gcccgcggtg ctcccgctcg gccaccaggt aggcacgcgc cagcagcgcc atgtcggccc
     2101 tggggtgggc ggcgcggtgg ctgcggatca gcggctcgat cgccgggtcc cacggcggcc
     2161 cgccggtgcc ggagcgctga ccggaggcgc gggtgcgcag cggacggcga atcaccacag
     2221 gcaccctcca gaacggtcga ccggcgcacc cacgctgctg cgagtggagg tcaatagtat
     2281 tgggcacgtc cagccgcgcc tacgtcttcg gctgggacac ggtgtccggg tcgcgcctcc
     2341 gctgatgtcg aggaccatgc ttcttgaacg agcctgaggt ccacccgggc gctggcctca
     2401 gcgcccgttg ccgaacaggc cggcgagccg ggctgctggt gaccgcttcg tcccggagcc
     2461 gccagccggt ggcagcgccc ccgggccctg gcggtctccc tggtcctgct ccctgcccac
     2521 gtcccgcgcg aggggtgcgg caaccatggc gagggactgc gcggcggtga tcgcgtcggc
     2581 gagctgctgc gaccagtcgg cgtgggcggg gtccgcggca gcctcggtct gctcgaccag
     2641 gccggcgaag cggcgcgggt cgcgctggcc cgtcggcagg gcgagccagc gggcgcggat
     2701 cgcctccagc cgggcggcgg cgcggttgtc ggccagcagc tcggcttcga cctggaggtg
     2761 ggcgagggag gccacggcgg cgaacagcgc cgccgtgctg aggccgaccc acaactccaa
     2821 gcgggcgacc gccagcacgc tgccggcgat cccggcggcg aggatcagca gctgcaggcg
     2881 ccggcgccgc cgttcggcga ccgccacccg ggcgcggcag gcgccgagcc gctcggccag
     2941 gcgcagctcc aggtagccat tcgggtcgag ggggctgagg ccgtcgtcgc cggcggtggt
     3001 cccctcccac ggcggcggca gcgggccgct gtaggagggc acgccgccgg cggcgacctc
     3061 ggtctgcatc aggcccgcgt cgatcgcgcc gagccgaccc gccagggtcg cgtcccgctc
     3121 cgccgcgcga gcggcggtcc cggcgtcgct gtccgcgtgg ctgtccgcgt ggctgtccgc
     3181 gtcggagtcc gcgtcggcgg ggacggaggc gggggcgtac gggccggtgg cggtgcggta
     3241 gcggtacagc tcacgccggg cggcctcggc ggcggcgcgc agcagcggcc agcgccgccc
     3301 cggcccgaag cggtggccgg cgagcagcac caccgtggcc aggaacggca gcgccgccac
     3361 ggtccagtgc agcgccagat ccgccaccga gccggcgccg acccggccgc gcacggcgtg
     3421 ctggaccagc gcgaccagga ccgccgcgac gccgagcgcc agcggccagc ggtgcaggcg
     3481 gtcgtcggtg cgccgcagcc gcgcggcggc ggcgtggtag cgggcgaagc gctcccagcc
     3541 gagcttcagc accggcgcgt cctgcagttc ccaggcgagc cggcgtgcca gcgccgcgtc
     3601 c
//
LOCUS       DASWDC010000068         8906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1348728, whole genome shotgun sequence.
ACCESSION   DASWDC010000068 DASWDC010000000
VERSION     DASWDC010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348728"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..916)
                     /locus_tag="VF995_02195"
     CDS             complement(239..916)
                     /locus_tag="VF995_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9342413.1"
                     /translation="MNTEVEQAARLAKRSRGAVVLVLLVAVLAFGVAKARDTGSLRLK
                     PLVTPTHALAGGDGELLGKASARVLVEEYGDFQCAPCTTFEQQAGPTLQRLAAQGTIR
                     FVFHPVAFSGTDASRAAAAATCAGDEGKFWPFHDRLYRDAQQGQDGPGTDSKANLIAL
                     GAQVGLTDQRFTTCVQHGTYEPWARKLTVDATRRGVNGAPAVFVNGRTSTAYLTPDGL
                     LTAIQGG"
     gene            complement(913..1854)
                     /locus_tag="VF995_02200"
     CDS             complement(913..1854)
                     /locus_tag="VF995_02200"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX9342414.1"
                     /translation="MVGIVGLPLAGPAPGAVVPRPTVGHLLLGWSFDPLVAVPLLAAA
                     ALYVHSWRRLRARGRRWPTQRLACFLCGLGAIALALQSPLEGYDTVLFSVHVTQHLLL
                     AMAAAPLLVLGAPITMLLMTVGTPTRKRVVRIVHSRPIQVIGHPLIAWVLFTLTLYGL
                     YFSPLFAVTLRNPFVHDLIHLHFILVGLLFWWPVVGIDPTRWRLHHLARVLYVFLMVP
                     FHAFLGVAIMGSGHLLAPALGQFTRDWGPAPLADQQAGGAIMWGAGDVISVVSVIAIL
                     AAWAQHEEKVAAREDRRLARARAAAAAAAATAERNQP"
     gene            complement(1997..2281)
                     /locus_tag="VF995_02205"
     CDS             complement(1997..2281)
                     /locus_tag="VF995_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX9342415.1"
                     /translation="MVRGYSDHKDAVRQRLRRVEGQVRGLQRMVEEDAYCIDVLTQIS
                     AVTRALQAVGLELLGDHLGHCVREAIAGGGPEADAKVAEASAAIARMVRS"
     gene            2420..3640
                     /locus_tag="VF995_02210"
     CDS             2420..3640
                     /locus_tag="VF995_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9342416.1"
                     /translation="MDLALSTLGDVAAIDPFAPEYRADPHPIYHRLRQLDPVHRLPGT
                     RDWTVFRHADATAVLRDPRFSADPAHLPPDAQPIAGPRRVESKVLLFLDPPNHTRIRS
                     LVSAAFTPARVRRLRTRVEELVSDLLDRAEAKGSLEVIGDLAYPLPVAVICELLGVPA
                     DADQATFRRWASDATRRLDGELDVDPESWARGEEAVASFIQYFITLINQRREQPEDGL
                     LDALIAAEQAGDRLDPEELLAIVILLFLAGHETTMNLIGNGLWALLRHPDQLERLRRD
                     PSLGPGAVEELLRYDGPVHVTSRIATEDLRVAGTPIRKGEQVIVVLAAANRDPAVFAD
                     PDRLDLGRRENRHLTFSAGMHYCLGAALAQLEGEVAIGALLRRFPTLALAGPEPVYRD
                     HFVLRGLRSLELSV"
     gene            3677..4411
                     /locus_tag="VF995_02215"
     CDS             3677..4411
                     /locus_tag="VF995_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342417.1"
                     /translation="MRRSRSRGNHRRPGGVPTSRLGVRSRGWDPAGPPTLRRSLTPGV
                     SLRLPLAAAAIAAAVAGHALTYLLTVPAAQARALLLAESGHGYWPTAVAAATVLGVIS
                     LVSTAARHFLRGFRRSARALAPGGPLARPKRLEWLALRLALLQISIYAVQEVVERLAA
                     GAPIAGVLHDGVLPIGILVQILVACAVALALAFLSHAAEAAGRALARLIARPSGTAVD
                     HRPVPRLHASSRHAGSLGSRAPPVVA"
     gene            4492..5151
                     /locus_tag="VF995_02220"
     CDS             4492..5151
                     /locus_tag="VF995_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342418.1"
                     /translation="MINRLAPIAAAIGVAALLVPLAATPAFAHEARDFSVFHVEVGFG
                     DEPPYIGDKNSVQLLLSSKDGKPVTDLGDSLQVEVGTGGNPPMKLAMEPNFEVGGDGT
                     PGDYRAWFFPTEPGKYTFHFTGTIHGRKVDQSFTSSPTGFDEVKDPSGVEYPAKDPTS
                     GQVAARLDRETGRLTSAAASAHDAANSAKTLAVVGIVVGVVGLLAGGALGGLGLRRKK
                     A"
     gene            5156..7336
                     /locus_tag="VF995_02225"
     CDS             5156..7336
                     /locus_tag="VF995_02225"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX9342419.1"
                     /translation="MRRPLRLLLRPPFAAAALLVLAGLWLLASALPASAHALLRSSDP
                     AAGADLERAPRQVLLTFTERPDPGLSVVHVLDSTGRPVEGGRAQLVPGHPLQLAVPLG
                     PLGKGAYTVTWRTVSRTDGHVTAGSFAFGIGVAAPTAANAGTVSTGPPPDWIAIVGRF
                     FFDAGTIVLLGAAAAGLLVFRPAGTFSPSRWLLGGALAAAVAGLVVQVAAERSRVGVS
                     FGKLLSSPTGRALLGQAAALVVVVAAVALLLRFRDRVGRGRTASLALLGGGAAAAMLL
                     HADGGHASSKPLDLLVQWAHLLAVGVWIGGLAWLLLGLRGAPAPASAAARFSRLATAA
                     ILVVAATGLTRAVAETGSWSGLFHTSFGHTLLLKVGLFAVVGALGAANRFILVPRLTR
                     AANPAAASRVGVPVGNPAAGGPGRTLPANPAGTAPGDAAGGPGAPASSASSGGGRAAG
                     VLRRTVAGELTVAVPLLLVAALLSELPPASYVTATKARAPAQVSVTGNDYATSVRLTL
                     TATPGAAGINVFQARVVDYDSRKPVTAARVSLRFTLPSRPDVGEATLDLKQQAGAAGA
                     WGGQGGQLSIAGSWSVTALVQLGGQALTVSLTVPVRQAPAPPGQQGVQVLRQPGQPTI
                     YQVPQPGGASIQAYLDPGKAGPDNDLHMTWFTASGSELPVAGATVTATSPAGVPQTLR
                     LIRFGAGHLAATGLKLTPGAWRFQINATAKAGGALPSSFQEVIQ"
     gene            7333..7809
                     /locus_tag="VF995_02230"
     CDS             7333..7809
                     /locus_tag="VF995_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342420.1"
                     /translation="MTKQGNGERTRARRLLLTTALAVAVALVAAGCGGRSGSGSGGGQ
                     TTTTPTLGPRPSTSAKLSILSPRDGQVVHGTTVDLKLRLQGAKIVAVVSKDLRPDRGH
                     VHVRLDGQLVSMTFGLEQKIPSLKPGTHVLQVEFVATDHLPFDPRVLAQTAFQVRS"
     gene            7806..8357
                     /locus_tag="VF995_02235"
     CDS             7806..8357
                     /locus_tag="VF995_02235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9342421.1"
                     /translation="MTPVVFAAGVAFLLARPSLEALPRGGVALLAAGYLSLDVMALPV
                     RLPEPRGEKARPGTLPVLPVLAAGIAAVGLAALLAGPAPALPGGAAPAALALDLVAAV
                     AEEALFRRVLFGWLARWSVPLAVVGSAAAFALVHVPLYGSAALPVDFGAGLLFSWQRA
                     ATGRWTVPAATHAAANLLAVIIR"
     gene            8354..>8906
                     /locus_tag="VF995_02240"
     CDS             8354..>8906
                     /locus_tag="VF995_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9342422.1"
                     /translation="MNAPLNLSRGGASLLAAALAVTLAAAACSGSGARNGAGPPVRVG
                     LMYPMSGSQGPGGLEEERGAQLAAKLANNTRGVAGRPIELVPLDVPEAGAVPAAIQSF
                     ASKGIRLVAGSYGSTISAPAAAAASARGMLFWETGAVGELFLPTDEQGDLVFQVAPSG
                     AVLGRSAIQFVAEHIAPSLHRDPS"
BASE COUNT         1107 a         3458 c         3121 g         1220 t
ORIGIN      
        1 tcgatcgtcc cgccgcctga cgtcagcctg ctgtccggcg accacgtccg gatcgcgatc
       61 aacggcctcg gcgagctggc caacccggtc gaagcggtcg gcttcgaagc cacgccctcc
      121 tagagcaacc accccgaccc cactcgacga ccacactcga cacctcagta ggggtgagct
      181 gcgagccgga gccgccggat ccaccacgcc aaggcgcagg ctcgcacccg gggtatccct
      241 accccccttg gatcgcggtg agcaggccgt ctggcgtcag gtacgcggtg gaggtcctgc
      301 cgttgacgaa gaccgccggg gcgccgttca cgccccggcg ggtggcgtcg acggtgagct
      361 tgcgcgccca gggctcgtag gtgccgtgct ggacgcaggt ggtgaaccgc tggtcggtga
      421 gacccacctg cgcgcccaga gcgatcaggt tggccttgct gtcggtgccc gggccgtcct
      481 gcccctgctg cgcgtcgcgg tagaggcggt cgtggaaggg ccagaacttc ccctcgtcgc
      541 ccgcgcaggt cgcggcggcg gcggcgcggg atgcgtcggt cccgctgaag gcgaccgggt
      601 ggaacacgaa gcggatcgtg ccctgggcgg cgagccgctg gagggtcggg ccggcctgct
      661 gctcgaaggt ggtgcagggg gcgcactgga agtcgccgta ctcctccacc agcacccgag
      721 ccgacgcctt gccgaggagt tcgccgtcac cgccggcgag tgcgtgcgtg ggcgtgacca
      781 gcggcttgag gcggagtgag cccgtgtcgc gggccttcgc gacgccaaag gcgagcacgg
      841 ccaccagcag cacgagcacc accgcgccgc ggctccgttt ggccagccgc gcggcctgct
      901 cgacctccgt gttcacggct ggttccgctc ggcggttgcc gcggcggcgg ctgccgcggc
      961 gcgggcgcgg gcaaggcggc ggtcctcgcg ggcagccacc ttctcctcgt gctgggccca
     1021 ggcggcgagg atggcgatca ccgacaccac cgagatgacg tccccggcgc cccacatgat
     1081 cgcaccgccg gcctgctggt cggcgagtgg cgcgggcccc cagtcgcgcg tgaactggcc
     1141 gagggcgggg gcgagcaggt gcccgctgcc catgatcgcc acccccagga aggcgtggaa
     1201 cggcaccatc aggaacacat acagcacccg ggccaggtgg tgcagccgcc agcgggtcgg
     1261 gtcgatcccc accaccggcc accagaacag cagcccgacc aggatgaagt gcaggtggat
     1321 caggtcgtgg acgaacgggt tgcgcagcgt gaccgcgaac agcggcgaga agtagagccc
     1381 gtacagggtc agggtgaaca gcacccaggc gatcagcggg tggccgatga cctggatcgg
     1441 gcggctgtgg acgatccgta cgacccgttt tcgggtggga gtgccaacgg tcatcagcag
     1501 catggtgatc ggcgcgccca ggacgagcag cggcgccgcc gccatcgcca gcagcaggtg
     1561 ctgggtgacg tggaccgaga acaggacggt gtcatagccc tccagcggcg actgcagcgc
     1621 cagcgcgatc gcccccagcc cgcagaggaa gcaggccagc cgctgggttg gccagcgccg
     1681 cccccgggcg cggagccgcc gccaggagtg gacgtagagc gcggccgccg ccagcaacgg
     1741 caccgccacc agcggatcga acgaccagcc gagcagcagg tgcccaaccg tcggccgggg
     1801 caccaccgcc ccgggcgccg gtccagccag cggcagcccc acaatcccca ccatcgtttc
     1861 gcccttgctc ccctcggccg atcctcacgg ccggcatctc atggtaggcc gggcgagatg
     1921 agtctgtggc ggttatccac aggggcctgg ggggtgctca gcggtgccca gtcgcccagg
     1981 cgatcgccgc cttcagttac gaacgcacca tcctcgcgat cgccgcggac gcctcggcga
     2041 ccttggcgtc ggcctcgggc ccgccgccgg cgatcgcctc acgcacgcag tggcccaggt
     2101 gatcgccgag cagctccagc ccgaccgcct gcagtgcccg ggtcacagcg gagatctgcg
     2161 tcagcacgtc gatgcagtag gcgtcctcct ccaccatgcg ctggagcccc cgcacctggc
     2221 cctccacccg gcgcagccgc tgccgcaccg cgtccttgtg gtcgctgtag cctcgaacca
     2281 ttcccgccct gcctttcgac cgcttcagac ggcgacgcca taccccccaa tggtatatgc
     2341 ggcgagcgag gcttccgctt gaggtcagcg ccccgcgggt gttcactggc caggcagggc
     2401 tgcaccccgg aggtgacgta tggacctcgc gctctccacg ctcggcgacg tcgcggccat
     2461 cgacccgttc gctcccgagt accgcgccga cccccacccc atctaccacc ggctgcgcca
     2521 gctcgacccg gtgcaccgcc tccccgggac ccgcgactgg acggtgttcc gccatgccga
     2581 cgccaccgcc gtcctgcgcg acccccgctt cagcgccgac cccgcgcacc tgccgccgga
     2641 cgcccagccg atcgccggcc cgcgacgggt cgagagcaag gtgctgctgt tcctcgaccc
     2701 gcccaaccac acccgcatcc gttcgctggt ctcggccgcc ttcacccccg cccgcgtgcg
     2761 ccgcctccgg acccgcgtcg aggagctggt cagcgacctg ctcgaccggg ccgaggccaa
     2821 gggcagcctc gaggtcatcg gcgacctggc ctacccgctg ccggtcgcgg tcatctgcga
     2881 gctgctcggc gtgcccgccg acgccgacca ggcgaccttc cgccgctggg cctccgacgc
     2941 gacccgccgc ctcgacggcg agctggacgt cgacccggag agctgggcac ggggggagga
     3001 agcggtcgcc agcttcatcc agtacttcat caccctcatc aaccagcgcc gcgagcagcc
     3061 cgaggatggc ctgctcgacg cgctgatcgc cgccgagcag gccggcgacc gcctcgaccc
     3121 cgaggagctg ctcgcgatcg tgatcctgct gttcctcgcc ggccacgaga ccaccatgaa
     3181 cctgatcggc aacggcctgt gggcgctgct gcggcacccc gaccagctgg agcgcctgcg
     3241 gcgcgaccct tcgctcggcc ccggcgcggt cgaggagctg ctccgctacg acggcccggt
     3301 gcacgtcacc tcccggatcg ccaccgagga tctccgggtc gccggcaccc cgattcgcaa
     3361 gggcgagcag gtgatcgtgg tcctcgccgc cgccaaccgc gacccggcgg tgttcgccga
     3421 tcccgaccgt ctcgacctcg gccgtcgcga gaaccggcat ctcaccttct ccgccggcat
     3481 gcactactgc ctcggcgccg ccctggcaca gctggagggc gaggtggcga tcggcgcgct
     3541 gctgcgccgc ttccccacgc ttgcgctggc cggcccggag ccggtctacc gcgaccactt
     3601 cgtgctgcgc gggctacgat cgctggaact gtcggtttag cgcctgacca cgttacgatc
     3661 tcccaagggg tgagctgtga gacggagccg gagccgcggg aaccaccgta ggcccggcgg
     3721 ggtcccgacc tcgcgacttg gagttcgtag cagaggttgg gaccccgccg ggccacccac
     3781 gctgcggcgc agtctcacac ccggggtatc ccttcgcctc ccgctcgctg ccgcggccat
     3841 cgccgcggcg gtcgccggcc atgcgctgac ctacctgctg acggtcccgg cggcgcaggc
     3901 gcgggcgctg ctgctggccg agagtggtca cggctactgg ccaaccgcgg tcgccgccgc
     3961 gacagtgctc ggcgtcatct ccctggtctc gaccgccgcg cgccacttcc tgcgcgggtt
     4021 ccgccgctcc gcgcgagccc ttgcaccggg tggcccgctg gcacggccga agcggctgga
     4081 gtggctcgct ctccggctcg cgctgctgca gatctccatc tacgcggtcc aggaggtcgt
     4141 cgagcggctc gctgccggcg ccccgatcgc cggggtgctg cacgacgggg tcctgccgat
     4201 cggcatcctc gtccagatcc tggtcgcctg cgccgtcgcg ctggctctgg ccttcctcag
     4261 ccacgccgcc gaggcagccg gccgcgccct cgcccgcctg atcgcccgcc ccagcggcac
     4321 tgcggtcgac caccggcccg tccccaggct ccacgcctcc tcccgccacg ccggctcgct
     4381 cggcagccgc gccccgccag tcgtcgcgta atcccgcaac cccggaatcc cgcagggcgc
     4441 ggtacctggc agcgcgccag gtgtcgcagt ggacaacgag aaggggatca cgtgatcaac
     4501 agactcgcgc ccatcgccgc cgcgatcggc gtcgccgcgt tgctggtccc gctggccgcg
     4561 acgccggcct tcgcgcatga ggcgcgcgac ttcagcgtct tccatgtcga ggtcggcttc
     4621 ggcgacgaac cgccctatat cggtgacaag aacagcgtcc agctgctgct gtcgtccaag
     4681 gacggcaaac ccgtcaccga cctcggcgac agcctccagg tcgaggtcgg caccggaggc
     4741 aacccgccga tgaagctggc catggagccg aacttcgagg tcggcgggga cggcaccccg
     4801 ggcgactacc gcgcctggtt cttcccgacc gagcccggca agtacacctt ccacttcacc
     4861 ggcaccatcc acggccgcaa ggtcgaccag tccttcacct cctcgcccac cggcttcgac
     4921 gaggtcaagg acccctccgg cgtcgagtac ccggccaagg acccgaccag cggccaggtc
     4981 gccgcccgtc tcgaccgcga gacgggccgc ctgaccagcg ccgcggccag cgcccacgac
     5041 gccgccaaca gcgccaagac gctggcggtc gtcgggatcg tggtcggcgt cgtcggcctg
     5101 ctggccggcg gtgccctcgg cggcctcggc ctgcggcgga agaaggcgta ggacgatgcg
     5161 gcgcccgctt cgcctcctcc tgcgcccgcc gttcgccgcc gcggcgctgc tggtcctcgc
     5221 cgggctgtgg cttctggcca gcgcgctgcc ggcgagcgcg cacgcgctgc tgcgctcctc
     5281 ggacccggcc gccggggcgg acctggagcg ggcgccgcgg caggtgctgc tcaccttcac
     5341 cgaacggccc gacccgggcc tgtccgtcgt ccacgtgctg gactcgaccg gccgtccggt
     5401 ggagggcggg cgcgcccagc tggtgccggg acacccgctc cagctggccg tgccactcgg
     5461 ccccctcggc aagggcgcct acacggtgac ctggcggacg gtctcgcgca ccgacggcca
     5521 cgtcaccgcc gggtcgttcg ccttcgggat cggcgtggcc gccccgacag cggccaacgc
     5581 cggcacggtc tcaaccggcc cgccacccga ctggatcgcg attgtcggcc gcttcttctt
     5641 cgacgcgggg acgatcgtgc tgctgggcgc ggctgccgct gggctgctgg tgttccgccc
     5701 ggcggggacc ttctcgccgt cccggtggct gctcggcggc gcgctcgccg ccgccgtcgc
     5761 cgggctggtc gtccaggtcg cggccgagcg gtcgagggtt ggcgtgtcgt tcggcaagct
     5821 gctctcctcg ccgactggcc gcgcgctgct cggccaggcc gccgcgctgg tggtggtggt
     5881 cgcggcggtg gcgctgctgc tccgcttcag ggaccgcgtc gggcgcgggc gcaccgcctc
     5941 gctcgccctg ctggggggtg gcgccgcggc ggccatgctg ctgcacgccg acggcggcca
     6001 cgcctccagc aaaccgctcg acctgctggt ccagtgggcc cacctgctcg cggtcggggt
     6061 ctggatcggc ggtctcgcct ggctgctgct cggcctgcgc ggcgcccccg cgcccgcgag
     6121 tgccgctgcc cgtttctccc ggctcgcaac ggccgcgatc ctggtggtcg cggcgaccgg
     6181 cctgacccgt gcggtcgccg agaccggcag ctggtccggc ctgttccaca ccagcttcgg
     6241 ccacaccctg ctgctcaagg tgggcctgtt cgcggtggtg ggcgccctcg gcgccgccaa
     6301 ccggttcatc ctggtgccac gcctgaccag ggccgccaac ccagcagccg ccagccgtgt
     6361 cggcgtcccg gtggggaacc cggcggcggg cggtcccggt cgcaccctcc cggcgaatcc
     6421 ggctggcacc gctcccggcg acgccgccgg cggtccgggc gccccggcca gctcggccag
     6481 ctcgggtggc ggccgcgcgg ccggggtcct gcgccggacg gtcgccgggg agctgacggt
     6541 cgcggtcccg ctgctgctgg tggccgcgct gctcagcgag ctgccacccg cgtcctacgt
     6601 gaccgccacc aaggcgcggg ctcccgcaca ggtcagcgtc accggcaacg actacgccac
     6661 cagcgtgcgc ctcaccctga ccgcgacccc cggcgccgcc gggatcaatg tcttccaggc
     6721 tcgcgtggtc gactacgaca gccgcaagcc ggtcacggcc gcgcgggtct cgctgcgctt
     6781 caccctgccg tcccggcccg acgtgggcga ggcgaccctg gacctgaagc agcaggcggg
     6841 cgccgccggc gcctggggcg ggcagggcgg ccagctctcc atcgccgggt cctggagcgt
     6901 caccgcgctg gtccagctcg gcggccaggc cctcaccgtg tcgctcaccg tccccgtccg
     6961 gcaggcgccc gcgccgccgg ggcagcaggg cgtccaggtg ctgcggcaac ccgggcagcc
     7021 caccatctac caggtcccgc aacccggcgg cgcctcgatc caggcctacc tcgaccccgg
     7081 caaggcgggc cccgacaacg acctgcacat gacctggttc acggcgtcgg gcagcgagct
     7141 gccggtcgcc ggcgccaccg tcaccgcgac cagcccggcg ggcgtccccc agacgctcag
     7201 gctgatccgc ttcggcgccg ggcacctcgc cgccaccggc ctgaaattga cccccggcgc
     7261 ctggcgtttc cagatcaacg cgaccgccaa ggcaggcggc gccctccctt ccagcttcca
     7321 ggaagtgatc cagtgaccaa gcaaggaaac ggggagcgca cccgagcccg gcgcctcctg
     7381 ctcaccaccg ccctcgccgt cgccgtcgcg ctggtcgcgg ccggctgcgg gggcaggtcc
     7441 gggtccggct ccggcggcgg tcagaccacg accaccccca cgctcggccc gaggccgtcg
     7501 accagcgcca agctgagcat cctgtcgccc cgcgacggcc aggtggtcca cggcaccact
     7561 gtcgacctga agctgcggct ccagggggcc aagatcgtcg ccgtggtcag caaggacctg
     7621 cgccccgacc ggggacacgt ccacgtccgc ctcgacggcc agctggtctc gatgaccttc
     7681 ggcctggagc agaagatccc cagcctcaaa cccggtaccc acgtcctgca ggtcgagttc
     7741 gtcgccaccg accacctgcc cttcgacccg agggtgctcg cccagacggc gttccaggtg
     7801 cggtcgtgac gcctgtcgtg ttcgccgctg gagtggcgtt cctgctggca cggccgtcgc
     7861 ttgaggcgct cccgcgcggg ggggtggcgc tgctggcggc ggggtacctc agcctggacg
     7921 tgatggcgtt gcccgtccgg ttgcctgagc cgcgggggga gaaggcccgg cccggcaccc
     7981 tcccggtgct tccggtgctc gccgccggga tcgcggcggt cgggctggcg gcgctgctgg
     8041 cgggcccggc gccggcgctg ccgggtggtg cggcaccggc ggcgctggcg ctcgacctgg
     8101 tcgccgcggt cgccgaggag gcgctgttcc ggcgggtgct gttcggctgg ctggcgcgct
     8161 ggagcgtgcc gctggcggtg gtcgggtcgg cggccgcgtt cgcgttggtg cacgttccgc
     8221 tctacggtag tgcggccctg ccggtcgatt tcggtgcggg gttgctgttc tcctggcagc
     8281 gcgcggccac cgggcggtgg accgtgcccg cggcgaccca tgccgcggcg aatctcctgg
     8341 cggtgatcat ccggtgaatg ctcccctgaa cctctcccgt ggcggcgcgt cgctgctggc
     8401 ggcggcgctg gccgtgacgc tggcggccgc ggcctgctcc ggctccggcg cgaggaacgg
     8461 agcgggcccg ccggtccggg tcgggctgat gtacccgatg agcggctcgc agggccccgg
     8521 tggcctggag gaggagcgcg gggcgcagct cgccgccaag ctcgccaaca acacccgcgg
     8581 ggtggccggc cggccgatcg agctggtccc cctcgacgtg cccgaggccg gcgccgtccc
     8641 cgccgcgatc cagtcgttcg ccagcaaggg gatccggctg gtggccggca gctacggcag
     8701 caccatctcg gccccggccg ccgccgcggc cagcgcccgg gggatgctgt tctgggagac
     8761 cggcgcggtc ggcgagctgt tcctgcccac cgacgagcag ggcgacctgg tgttccaggt
     8821 ggcgccctcc ggggcggtgc tgggccgctc cgccatccag ttcgtggccg agcacatcgc
     8881 gccgagcctg caccgcgacc cgtcca
//
LOCUS       DASWDC010000069         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_847955, whole genome shotgun sequence.
ACCESSION   DASWDC010000069 DASWDC010000000
VERSION     DASWDC010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847955"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VF995_02245"
     CDS             <1..253
                     /locus_tag="VF995_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342423.1"
                     /translation="SVGLVAGPRVASEAAAVAPQVAADDPGAAGFGGGAGAPAWSTRL
                     ELRTEPDPDLERFDDYLVAAEALAEAYGGEVVDRATYPL"
     gene            406..735
                     /locus_tag="VF995_02250"
     CDS             406..735
                     /locus_tag="VF995_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342424.1"
                     /translation="MASTTWPEPVRRVVAEAEAGATMLIGLHSARPFERWPLGRVTLL
                     GDAAHSLSPARGRGRHPAPAVRAGAAPGGLSPPAEATGPPRLVAGAELPQAVGQGLDA
                     ATLPPSR"
     gene            complement(766..1815)
                     /locus_tag="VF995_02255"
     CDS             complement(766..1815)
                     /locus_tag="VF995_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX9342425.1"
                     /translation="MTRARIVLLASAGWANRGIARKLGLAPNTVGKWRKRFFAEGVDG
                     LRDRKRPGRPRRFPAAVVAACKAIACELPATRGVPLGRWSLAELRTEVLATGLVDDVS
                     TTTLWRWLDEDPIKPWQHRSWIFPRDPDFTAKAGLVLDLYQRLFAGQTLGDDEYVVSA
                     DEKTSIQARCRCHPTLPPGRARMMRVEHEYERGGALAYLAAWDVHQATLFGRCEQTTG
                     IAPFGRLVAQVMTSQPYASAKRVFWVVDNGSSHRGRASVKRLEGEWRNLCLVHLPIHA
                     SWLNQIELVFSVIQRKVLTPNDFTDLAEVEARLLAFQRRYQQTADPFDWRFTRADLHR
                     LLQRLDEPEHLGTAA"
     gene            2009..2368
                     /locus_tag="VF995_02260"
     CDS             2009..2368
                     /locus_tag="VF995_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342426.1"
                     /translation="MSKPIPEPTKTSLGQRLRAQARERWPALAGVQVRFRGRFAYVDG
                     ELSGGEGLPLCRLRYSGSASLWGFAIYLASRDGYEDSVLPSGLPVGTPEEALDCACGL
                     YLADPSVWQNQLPPADP"
BASE COUNT          362 a          955 c          953 g          366 t
ORIGIN      
        1 gtcggtcggg ctcgtggccg ggccgagggt cgcctccgag gcggccgcgg tcgcgcccca
       61 ggtggccgcc gacgacccgg gcgccgccgg cttcggcggg ggggccgggg caccggcctg
      121 gtccacccgc ctggagctgc gcaccgagcc ggacccggac ctggagcgct tcgacgacta
      181 cctggtcgcg gccgaggcgc tggccgaggc gtacggcggc gaggtggtcg accgggcgac
      241 ctacccgctc tagcatcgga cccggccggg cgggaccagc ggcgcggggg acgtggagcc
      301 cccccgactg ccgccggccc ctcgtcacgg cccgggcgca cgccgaccgt gcgaggcgag
      361 gcacctcaca cccctgccga ggtccctcgc cccaggaggc gtcccgtggc atccaccacc
      421 tggcccgagc ccgtccgccg ggtcgtggcc gaggcggagg cgggcgcgac catgctgatc
      481 gggctgcact cggcccggcc gttcgagcgc tggccgctcg ggcgggtgac cctgctcggg
      541 gacgccgccc acagcttgtc gccggcacgc ggccgtggac gccaccctgc gccagccgtt
      601 cgggccggcg cggcgcccgg cgggctgagc ccccccgccg aggccaccgg cccgccccgc
      661 ctggtcgccg gcgccgagct gccgcaagcc gtgggccaag gtctcgacgc ggcaacgctt
      721 cccccgagtc gctaagtggt ccgtcataga agttcgtgcg tggtgtcatg ctgcggttcc
      781 aaggtgctcg ggttcgtcca gccgctggag gagccgatgg aggtcggcgc gggtgaagcg
      841 ccaatcgaac gggtcggcgg tctgctggta gcggcgctgg aaggcgagca ggcgagcttc
      901 gacctcggcg aggtcggtga agtcgttggg ggtcagcacc ttccgctgga tcaccgagaa
      961 gacgagctcg atctggttca gccaggaggc gtggattggc aggtggacca ggcacaggtt
     1021 gcgccattcg ccctcgaggc gcttgaccga tgctcgcccc cggtgggagc taccgttgtc
     1081 gaccacccag aacacccgct tggccgaggc gtagggctgg ctggtcatga cctgggcgac
     1141 cagccggccg aacggggcga tgccggtcgt ctgctcgcag cggccgaaca gcgtggcctg
     1201 gtggacgtcc caggccgcca agtaggccag cgcaccgccg cgctcgtact cgtgctcgac
     1261 ccgcatcatg cgggcccggc ccggcggcag cgtggggtgg cagcggcagc gcgcctgaat
     1321 actcgtcttc tcgtcggcgc tgacgacata ctcgtcgtcg ccgagcgtct ggccggcgaa
     1381 cagccgctgg tagaggtcca ggacgaggcc tgccttggcg gtgaagtcgg ggtcgcgggg
     1441 aaagatccac gagcggtgct gccacggctt gatcgggtcc tcgtccagcc agcgccacag
     1501 cgtcgtggtg gagacgtcgt cgactaggcc ggtggccagg acctcagtcc gtagctcggc
     1561 gaggctccag cggccaagcg gtaccccgcg ggtggcgggc agctcacagg cgatcgcctt
     1621 acaggcggcc accaccgccg cagggaaccg gcgtgggcgg ccaggccgct tgcggtcacg
     1681 aaggccgtca acgccctcgg cgaagaagcg tttgcgccac ttgccgaccg tgttgggcgc
     1741 cagcccgagc ttgcgggcga tccctcgatt ggcccatccc gccgacgcca gcagcacgat
     1801 cctggccctg gtcacctggc ggtgctcggc cgtgccctgg cgcgtcaggg ccttcaacca
     1861 ccggcgctgc gacgccccca gcgtgatctg gtatgggctg cgcctgccac cttgtgccac
     1921 catccctcca gtccccaagc cggccccgcc ggccggcctg gcggaggata cggggatgcg
     1981 accaggcgcc atcagcgaag atgggtcgat gtccaagccc atccccgagc cgaccaagac
     2041 ctcactcggc cagcggctcc gcgcccaggc tcgcgagcgc tggcccgcgc ttgccggcgt
     2101 ccaggtgcgc ttccggggcc gcttcgccta cgtcgacggc gagctcagcg gcggggaggg
     2161 gctgccgctg tgccggctgc gctacagcgg ctcagcgagc ctgtgggggt tcgccatcta
     2221 cctggccagc cgggacggct acgaggattc ggtgctgccc agcggcctgc cggtcggcac
     2281 gcccgaggaa gccctggact gcgcctgcgg gctctacctt gccgacccct ccgtctggca
     2341 gaaccagctc ccacccgctg atccctgacc acgcacgaac ttccctacgg cagcacttag
     2401 tcacctgagg tagatcatcg cgattctcca tccgctggcg gcgatcaggc ggagggcaat
     2461 tggaaagttc tatggctaca ccatcacgcc cgggcaccgc gggcgatgat cagacgcgcg
     2521 aggcgtccgc gaacaactcc gcggcaggcc gagcgcctgg accgcagtcg gtttctggat
     2581 ctcggtcaag atcgcgtcca gggtcaaccc accagagcgg cgctcgggtc acgcca
//
LOCUS       DASWDC010000070         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1599985, whole genome shotgun sequence.
ACCESSION   DASWDC010000070 DASWDC010000000
VERSION     DASWDC010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599985"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2245
                     /locus_tag="VF995_02265"
     CDS             <1..>2245
                     /locus_tag="VF995_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX9342427.1"
                     /translation="AERLTGEVGQADLYAVTPPDFTRRLGLRDAWLEHATRSVRRKLA
                     ERLAFQAHLEFGLRSRLGRTLELTGALVAEVDADLLHLAALIRELHAHLPEATLARPD
                     PRAYLVWLLGLLDRLRTRGGILHPWLDRYIQLDGNRWPIWGGRKPGMPAFPRGVGAPA
                     FYTTAPSSEPFDSLAPHGEQATWLTDWTVRCLQIPAREAKALLLQTATALGGDGLGVL
                     ARRSTRSGATVYGLPPERIAVQRVADEDLASGRTRLACGVCRHVQPSAPERYELRQGS
                     PCPRFRCPGRLEPAPGDAGNFYRKLYRSGRIRRIVAQEHTSILDRDERERIERRFKAG
                     DSPVDPNILACTPTLELGIDIGDLSAVTLASVPRRPANYLQRVGRAGRRSGNAFILAA
                     VPSTARDLYWFAEPRHLIAGDVTPPSCYLDATELLCRQFLAWCIDRAAAGELAVGSRM
                     PHLTGQLVRGGLEPEGWMRRLLDAVHAGHQGLAGDFLALFGDHLLEASREQVRAFAAA
                     GVEVAVSQAFADWNRRERELQDRLIRVQEAIIELEARRDSLEDEERADLRRLIGEKGA
                     LGRLHKTMSSEYALSGLEELGLLPNYNLLDDATVLDVALWWTSEDEGDGEKHRIAQYS
                     YTRGSMTALSELAPGAVFYVAGQRIRVDAVDVGPLDQPLWRPWRLCPECGWGTDEAEC
                     PPSSCPRCASVHVADTGAVHNLLPLERVSAVQSRDDALIDDDQEDRQRVGFTLLTQVD
                     ADPAEVV"
BASE COUNT          294 a          876 c          785 g          290 t
ORIGIN      
        1 tcgccgagcg cctcaccggg gaggtcggcc aggccgacct gtatgcggtc accccgccgg
       61 acttcacccg ccgcctcggc ttgcgggacg cctggctgga gcacgcgacc cgttcggtgc
      121 gccgcaagct ggcggagcgg ctggcgttcc aggcgcacct ggagttcggc ctgcgcagcc
      181 gcctcggccg caccctggag ctgaccggcg cgctggtcgc cgaggtggac gccgacctcc
      241 tccacctcgc cgcgctgatc agggaactgc acgcccacct gcccgaggcc accctggcac
      301 gccccgaccc ccgcgcctac ctggtctggc tgctcggcct gctcgaccgg ctccgcaccc
      361 ggggcggtat cctgcacccc tggctcgacc ggtacatcca gctcgacggc aaccgctggc
      421 cgatctgggg cggccggaag cctggcatgc cggccttccc caggggcgtg ggcgcgcccg
      481 cgttctacac caccgcgccc tcttccgagc cgttcgactc gctggcgccc cacggcgagc
      541 aggcgacctg gctgaccgac tggaccgtcc gctgcctcca gatccccgcc cgcgaggcca
      601 aggcgctgct gctgcagacc gccactgccc tcggcggcga cggcctcggc gtgctggccc
      661 gccgctcgac ccgctccggc gcgaccgtgt acggcctgcc gcccgaacgg atcgccgtgc
      721 agcgggtcgc cgacgaggac ctggccagcg gccggacccg cctcgcctgc ggcgtctgcc
      781 gccacgtcca gccctccgcc ccggaacgct acgagctgcg ccagggaagc ccctgccctc
      841 gcttccgctg ccccggacgg ctggagccgg cgcccggcga cgccggcaac ttctaccgca
      901 agctctaccg cagcgggcgc atccgccgga tcgtcgccca agagcacacc agcatcctcg
      961 accgcgatga gcgggagcgg atcgagcgcc gcttcaaggc cggcgactcc cccgtcgacc
     1021 ccaacatcct cgcctgcacc ccgacgctgg agctaggcat cgacatcggc gacctgtcgg
     1081 ccgtcacgct cgcctcggtg ccgaggcgcc cggcgaacta cctgcagcgg gttgggcgcg
     1141 ccgggcgccg cagcggcaac gccttcatcc tggcggcggt ccccagtacc gcccgcgacc
     1201 tgtactggtt cgccgagccg cgccacctga tcgccgggga cgtcaccccg ccgtcgtgct
     1261 acctggacgc caccgagctg ctgtgccgcc agttcctcgc ctggtgcatc gaccgggcgg
     1321 cggccggcga gttggccgtg ggcagccgca tgcctcacct caccgggcag ctggtgcggg
     1381 gcggcctgga gccggagggg tggatgcgcc gcctgctcga cgcggtccac gccggccacc
     1441 aagggctcgc cggcgacttc ctggcgctgt tcggcgacca cctcctggaa gcgtccaggg
     1501 aacaggtccg cgccttcgcc gcagcggggg tggaggtcgc ggtctcgcag gcgttcgccg
     1561 actggaaccg ccgcgagcgc gagctgcagg accgcctgat ccgtgtccag gaggcgatca
     1621 tcgagctgga ggcgcgtcgc gacagcctgg aggacgagga gcgcgccgac ctgcgccggc
     1681 tgatcggcga gaagggcgcc ctcggcaggc tccacaagac gatgagcagc gagtacgcgc
     1741 tcagcgggct ggaggagctg gggctgctgc cgaactacaa cctgctcgat gacgccaccg
     1801 tgctcgacgt ggcgctgtgg tggacgagcg aggacgaagg cgacggcgag aagcaccgga
     1861 tcgcccagta ctcctacacg cgcggcagca tgacggcgct gtcggagctg gcgcccggcg
     1921 ccgtgttcta cgtggccggg cagcggatcc gcgtcgacgc cgtcgacgtc gggccgctgg
     1981 accagccgct gtggcggccg tggcggctgt gcccggagtg cggctggggc accgacgagg
     2041 ccgagtgccc gccctcctcc tgccctcgct gcgcgtcggt ccacgtcgcc gacaccggag
     2101 cggtccataa cctgctgccc ctggagcggg tgtcggcggt gcagagccgc gacgacgcgc
     2161 tgatcgacga cgaccaggag gaccgccagc gggtcggctt caccctcctc acccaggtcg
     2221 acgccgaccc ggccgaggtc gtcag
//
LOCUS       DASWDC010000071         6252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1016530, whole genome shotgun sequence.
ACCESSION   DASWDC010000071 DASWDC010000000
VERSION     DASWDC010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016530"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VF995_02270"
     CDS             complement(<1..242)
                     /locus_tag="VF995_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9342428.1"
                     /translation="MARLVAISDAHLGRSHYQAVNEQGLNQREADFAAAFQRAVDAAL
                     DLNPDAVLVLGDLFDAPRPTYRAFREGAKGLRRLQQA"
     gene            complement(242..1819)
                     /locus_tag="VF995_02275"
     CDS             complement(242..1819)
                     /locus_tag="VF995_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9342429.1"
                     /translation="MADHPYKGRIFGRSVLDVRFRAFYGEDLFLGELLVGDDSDKGRR
                     YLFRVTDVAYGSESSDESWAERTAGGLMAMDDAGESTAARDLERRLYKVAKVSSLGYV
                     GPEGHFHKPKSLPGQFGMVTEPSDADLAFLSERMGDIEIGRLRSGEDTVDLPVGIRGE
                     TLPSHVGVFATTGMGKSNLMKVLSGQILAAAGRYALLLFDPHGEYLSGGGPGRRGLAH
                     HPWAQQRLRVYSPNPKAGEASLLRLSLAELSVDDLRTAWKFSGPQSEALESARALLGD
                     KGIWLQRLAEEEPEDLKDAELGRHALATIQVLCRRARRIVQLPLMTSDPSQSLTGKVL
                     DDLADGKVVLVDTSGLEAVEETLVASLLTRSLLEERATAYRTDREHFGRLPRTLVALE
                     EAQRVLTRLDDAEFNVFPRLAREGRKFNVGLCAVTQQPKLIDAELLSQFNTYFVLGLA
                     DERDRQILRSSSKQDLSDLGPEIQTLMPGEALVTNPEAPFALPTRVHLYEEWITTVAG
                     PEPAKARPDGALRGFYD"
     gene            complement(1852..2943)
                     /locus_tag="VF995_02280"
     CDS             complement(1852..2943)
                     /locus_tag="VF995_02280"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEX9342430.1"
                     /translation="MHREVQLLVHDYAERVRDAAGYLSRLLADDGTRVDNPTDERLAP
                     EGDQIELRPDAEIIEMGSDPGVGVTVAAIDGGSACLVNGRSFWVIAYRAGTIWHRDRC
                     TFNEDCDQLNFLALTQSDAKAVYADALRRNGVVRDRELSDLGGVVDVLRELAEWHRVT
                     AALEQAAPGDLVLVDGSLHPGAFLPPSLVTPVHQLALEREVDLVGVTKASKLRWGRYA
                     PLVLRVRRRAECEVGPDARWFLQVTGDPEAPDAADPPAEDIGGHGWRSEVYVARLARR
                     GPYAFRVDAVRGRRSSEELFALLAGLSDDPAFLGYPYPLARVHQVVALPGHLLADLRR
                     DLREACLRQGLSEDDVDLVMRDYHLTLNA"
     gene            complement(3052..3306)
                     /locus_tag="VF995_02285"
     CDS             complement(3052..3306)
                     /locus_tag="VF995_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342431.1"
                     /translation="MLSFNAIALLYLLGQVDLGIALAMQGRLDEAYGAGIVALDSPRQ
                     ADSLRSRMDTLDRLLTARYPDATETREFHERYLVAVNSAA"
     gene            3327..3761
                     /locus_tag="VF995_02290"
     CDS             3327..3761
                     /locus_tag="VF995_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342432.1"
                     /translation="MRCRPAGRKARGQLPTANIATPTGERFDSVQLRHATDPEAILSW
                     LADTAEVLAGPVIRSATTSLGFLVKPTQAGDSPAPPVPWLTYHGPGSLVLLPPGWAVD
                     CTELSWVRPLDLEVGAALPPGGPLLDALGRLPAEVRVRSPKL"
     gene            complement(3736..5004)
                     /locus_tag="VF995_02295"
     CDS             complement(3736..5004)
                     /locus_tag="VF995_02295"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9342433.1"
                     /translation="MLRRAGATNLGAAYHTLWTAAAISTLGDGVYLTGLPLLAASLTR
                     DPLRVSIVGAASWLPWVLLGLPAGALVDRWDRRGVMWRVDTGRFLVVGALAVVVLAGW
                     ASIWLLAAAGLLLGAGQVLFDSAAQAILPTMLARDPRRLERANSQLLGTQQVGEQLAG
                     RPLGGLLFSLATAVPFAVDAVSFAVSSLLLITIPGRFAVAAGDAPGAPRPRRRLRAEI
                     AEGLRWLIGHPLFRTMAVVVSVTNLSVSASDAILVLFALEDLKLHSVGFGLLLAGFAV
                     GGILGSVVATRVSRLIGPGAVFVSALLVGALALAGVGATSDPLVAGALLAVEGAVFTI
                     FNIVAITLRQAIVPDRLTGRVVATNRLIVFGTIPFGSILGGVLGRTISLRAPFLGGAA
                     VLLATALLALPRINNRTVNAAQLTASASEP"
     gene            5102..6079
                     /locus_tag="VF995_02300"
     CDS             5102..6079
                     /locus_tag="VF995_02300"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX9342434.1"
                     /translation="MLRTRACEVLGIEVPIVQGPLGGPWEQGMELPAAVCEAGGLGGV
                     PASLRDAAQLRRDMVRLRELTGRPFAVNHTRRPFSEEVFAASLEDPPAVISLALGAPG
                     DLARRAHDAGAVFLQQVTTVEQARQAAEAGVDVISAQGTESGGFSGDVSALALIPQVV
                     DAVAPIPVLASGGISDGRGLVAALALGAQGINIGTRFLASEECAVPDAWKHAILTASS
                     QDAVKIAFATAILPSPTRGGYPTVPRALATPFVSEWNAHPAHAAVLADQLREQVAAAT
                     REGRAHELVPFTGQSAGMITSVLPAAEIIRTILAQAAVTLGTLAELAAR"
     gene            complement(6138..>6252)
                     /locus_tag="VF995_02305"
     CDS             complement(6138..>6252)
                     /locus_tag="VF995_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEX9342435.1"
                     /translation="LTAGAEAQQPISDVGGGKLIASVKDPDGNPIGLLQEA"
BASE COUNT          868 a         2113 c         2429 g          842 t
ORIGIN      
        1 gcctgctgca ggcggcgcag gcccttggcg ccctcgcgga aggcgcggta ggtggggcgg
       61 ggcgcgtcga acaggtcgcc gagcaccagc accgcgtccg ggttcaggtc cagggcggcg
      121 tccacggcgc gctggaaggc ggcggcgaag tcggcttcgc gctggttgag gccctgctcg
      181 ttgacggctt ggtagtggga gcggccgagg tgggcgtcgg agatcgcgac caggcgggcc
      241 atcagtcgta gaacccccgc agggcgccgt cggggcgggc cttggcgggc tccgggccgg
      301 caacggtggt gatccactcc tcgtagaggt gcacccgggt cggcagcgcg aacggcgcct
      361 cggggttggt gaccagcgcc tcgccgggca tcagcgtctg gatctccggg ccgaggtcgg
      421 acaggtcctg cttggaggac gaccgcagga tctgccggtc gcgctcgtcg gccaggccca
      481 gcacgaagta ggtgttgaac tggctgagca gctccgcgtc gatcagcttg ggctgctggg
      541 tgacggcgca caggccgacg ttgaacttgc ggccctcccg cgccaggcgg gggaagacgt
      601 tgaactcggc gtcgtcgagg cgggtcagga cccgctgggc ctcctccagc gcgaccagcg
      661 tgcgcggcag gcggccgaag tgctcccggt cggtgcggta ggcggtggcg cgttcttcga
      721 ggaggctgcg ggtgagcagg gaggcgacca gggtctcctc gaccgcctcc agcccggagg
      781 tgtcgaccag cacgaccttg ccgtcggcga ggtcgtcgag gaccttgccg gtgagcgact
      841 ggctcgggtc gctggtcatc agcggcagct gcacgatccg ccgggcgcgg cggcacagca
      901 cctggatggt ggccagggcg tggcggccga gctcggcgtc tttcaggtcc tcgggttcct
      961 cttcggcgag gcgttgcagc cagatgccct tgtcgccgag gagcgcgcgg gcgctctcca
     1021 gcgcctcgga ctgggggccg ctgaacttcc aggcggtgcg gaggtcgtcg acggacagct
     1081 ccgccagcga caggcgcagc aggctcgcct cgccggcttt ggggttcggc gagtagaccc
     1141 gcaggcgctg ctgcgcccag gggtggtggg cgaggccgcg gcggcccggt cccccgccgc
     1201 tgaggtactc gccgtggggg tcgaacagca gcagggcgta gcggccggcg gcggcgagga
     1261 tctggccgga gaggaccttc atgaggttcg acttgcccat gccggtggtg gcgaagacgc
     1321 cgacgtggct cgggagggtc tccccgcgga tcccgaccgg gaggtcgacg gtgtcctcgc
     1381 cggagcggag cctgccgatc tcgatgtcgc ccatgcgctc gctgaggaag gcgaggtcgg
     1441 cgtcggacgg ctcggtgacc atgccgaact ggccggggag gctcttcggc ttgtggaagt
     1501 gcccctcggg gccgacgtag ccgaggcttg agaccttggc gaccttgtag aggcggcgtt
     1561 ccaggtcgcg ggcggcggtg gactcgccgg cgtcgtccat ggccatgagc ccgccggcgg
     1621 tgcgttcggc ccagctctcg tcggaggact cgctgccgta ggcgacgtcg gtgacacgga
     1681 acaggtagcg ccggcccttg tcgctgtcgt cgccgaccag cagttcgccg aggaacaggt
     1741 cctcgccgta gaaggcgcgg aagcggacgt cgagcacgga acggccgaag atccgtcctt
     1801 tgtagggatg gtctgccatg gatggtcctc tgttcctgtg ggtctgggcg gtcaggcgtt
     1861 gagggtcagg tggtagtcgc gcatcaccag gtcgacgtcg tcttcggaga ggccctggcg
     1921 caggcacgcc tcgcggaggt cgcggcgcag gtcggcgagc aggtggccgg ggagggcgac
     1981 gacctggtgg acgcgcgcca gcgggtaggg gtagccgagg aaggccgggt cgtcggaaag
     2041 cccggcgagc agcgcgaaca gctcctcgga ggagcgccgc ccgcggaccg cgtcgacccg
     2101 gaaggcgtag gggccgcgcc gggcgaggcg ggcgacgtag acctcggagc gccagccgtg
     2161 cccgccgatg tcctcggctg gagggtcggc ggcgtcgggt gcttcggggt cgccggtgac
     2221 ctggaggaac cagcgggcgt cggggccgac ctcgcattcg gcgcggcggc ggacccgtaa
     2281 caccagcggg gcgtagcggc cccagcggag cttggaggct ttggtcacgc cgaccaggtc
     2341 cacctcgcgt tccagggcga gctggtggac gggggtgacc agggagggcg gcaggaacgc
     2401 gcccgggtgc aggctcccgt cgaccagcac gaggtcgccg ggagcggcct gctccaaggc
     2461 ggcggtgacg cggtgccact cggccagctc gcggagtacg tccacgaccc cgccgaggtc
     2521 ggacagctcc cggtcgcgga ccaccccgtt gcggcgcagc gcgtcggcgt agaccgcctt
     2581 ggcgtccgac tgggtgaggg caaggaagtt gagctggtcg cagtcctcgt tgaaggtgca
     2641 ccggtcccgg tgccagatgg tcccggcgcg gtaggcgatc acccagaagc tgcgcccgtt
     2701 gaccaggcag gccgacccgc cgtcgatggc ggcgacggtg accccgacgc cggggtcgct
     2761 gcccatttcg atgatctcgg cgtcggggcg cagctcgatc tggtcccctt cgggcgcgag
     2821 gcgctcgtcg gtggggttgt cgacccgggt gccgtcgtcg gcgagcagac ggctgaggta
     2881 gccggccgcg tcccgcaccc gctcggcgta gtcgtggacg agcagttgga cctccctgtg
     2941 caccaagcgg accgaacaga cgttcccgag cgtaaccctc actcgggccc aggggaagca
     3001 ccggcggagt ctaccaaccg cccgcgacga aaccgcgccc cgaatgccgc ttcaggccgc
     3061 cgaattcacc gcaaccaggt agcgctcgtg gaattcacgg gtttccgtgg cgtccgggta
     3121 cctggcggtg agcaggcggt ccagggtgtc catgcgggag cggagcgagt cggcctgccg
     3181 tggtgagtcc agagcgacaa tgccagcgcc gtaggcctcg tcgaggcggc cttgcatcgc
     3241 gagagcgatg ccgagatcaa cttgtcccaa gaggtagagg agcgcaatgg cgttgaatga
     3301 caacatcgtt cctgggccga cagcgaatgc gttgtcgacc ggcaggtcgg aaagctcgcg
     3361 gacagctccc caccgccaac atcgccaccc cgactggtga gcgcttcgac agcgtccagc
     3421 tccgccacgc caccgatccc gaggcgatcc tcagctggct cgccgatacc gccgaggtcc
     3481 tcgccggccc ggtgatccgc tcggccacga cctccctcgg ctttctggtg aagccgaccc
     3541 aggccggcga ctcccccgcc ccaccggtcc cgtggctcac ctaccacggg cctggcagcc
     3601 tggtcctgct gccgcctggc tgggcggtgg actgtaccga gctgtcctgg gtccgccctc
     3661 tcgacctgga ggtcggtgcg gcgctgccgc ctggcggccc gctgcttgac gcgctcgggc
     3721 gcctcccggc ggaggtcagg gttcgctcgc cgaagctgtg agctgcgccg cgttgacggt
     3781 gcggttgttg atcctcggga gggcgagcag cgccgtcgcg agcaggaccg cggcgccgcc
     3841 caggaacggt gcgcgcaggc tgatggtacg gccgagcacg ccgccgagga tgctgccgaa
     3901 ggggatggtc ccgaagacga tcagccggtt ggtggcgacg actcgcccgg tgaggcggtc
     3961 aggcacgatc gcctggcgca gcgtgatcgc gacgatgttg aagatggtga acaccgcgcc
     4021 ctccaccgcc agcagcgcgc cggccacgag cgggtcggag gtggcgccga cgccggccag
     4081 ggccagcgct cccaccagca gcgccgacac gaacaccgcg ccggggccga tcaggcggct
     4141 gacgcgggtc gcgaccacgc tgccgaggat gccgccgacc gcgaagccgg ccagcagcag
     4201 gccgaagccg acgctgtgca gtttgaggtc ctccagggcg aacaggacga ggatcgcgtc
     4261 gctggccgag accgacaggt tggtgacact gaccaccacc gccatggtgc ggaacagcgg
     4321 gtggccgatc agccagcgca gcccctcggc gatctcggcc cgcaggcggc gccggggacg
     4381 cggcgcgccc ggggcgtcgc cggccgcgac ggcgaaccgg ccagggatcg tgatgagcag
     4441 cagcgagctg accgcgaacg agaccgcgtc gaccgcgaac gggaccgcgg tggccaggga
     4501 gaagagcagc ccgccgagcg gtctgccggc cagttgctcg cccacctgct gggtgccgag
     4561 cagctggctg ttggcgcgct ccagccgccg ggggtcccgg gccagcatgg tgggcaggat
     4621 cgcctgggcg gcgctgtcga acaggacctg cccggcgccg agcagcaacc cggcggcggc
     4681 gagcagccag atgctcgccc agccggcgag caccaccacc gcgagggcgc cgacgaccag
     4741 gaagcggccg gtgtcgaccc gccacatcac cccgcggcgg tcccagcggt ccaccagcgc
     4801 cccggcgggc aggccgagca ggacccaggg gagccagctg gccgcgccga caatcgagac
     4861 ccgcaggggg tcgcgggtga ggctggccgc cagcagcggc aggccggtga ggtagacgcc
     4921 gtcgccgagg gtggagatcg ccgcggcggt ccacagcgtg tggtaggcgg cgccgaggtt
     4981 ggtcgcacca gcgcggcgga gcatgcctca tggtaggtcg cggccccgga cttgtaacac
     5041 gccttccgct gcgagcgata gtgatgcagg aggcgtcgca gccggagtcg gggaggggcg
     5101 gatgttgcgg accagggcgt gcgaggtgct gggcatcgag gtcccgatcg tgcagggccc
     5161 gctcggcggg ccgtgggagc aggggatgga gctcccggcg gcggtgtgcg aggcgggcgg
     5221 gctcgggggc gtcccggcct cgctgcggga cgcggcgcag ctccgccggg acatggtgcg
     5281 gctgcgggag ctgaccgggc gcccgttcgc ggtcaaccac acccgccggc cgttcagcga
     5341 ggaggtcttc gccgccagcc ttgaggatcc gccggcggtg atctccctgg cgctcggggc
     5401 gccgggggat ctggcccgcc gggcgcatga cgcgggggcg gtgttcctgc agcaggtcac
     5461 caccgtcgag caggccaggc aggcggcgga ggcgggggtt gacgtgatca gcgcgcaggg
     5521 cacggaatcg ggcggcttca gcggcgacgt gagcgccctc gcgctgatcc cgcaggtggt
     5581 cgacgcggtg gcgccgatcc cggtgctcgc ctcgggcggc atctccgacg ggcgcggcct
     5641 ggtcgccgcc ctcgccctcg gagcgcaggg gatcaacatc ggcacccgct tcctggcatc
     5701 cgaggaatgc gccgtccccg acgcctggaa gcacgcgatc ctcacggcgt cgtctcagga
     5761 cgcggtcaag atcgccttcg ccaccgcgat cctgccctcc cctaccaggg gcggctaccc
     5821 gaccgtgccc cgcgcgctcg cgaccccgtt tgtgtccgag tggaacgcgc accccgccca
     5881 cgccgccgtc ctcgccgacc agctccgcga gcaggtcgcc gcggcaacgc gcgagggccg
     5941 cgcccacgag ctggtcccct tcaccggcca gagcgccggg atgatcacct cggtcctgcc
     6001 agccgccgag atcatccgca cgatcttggc ccaggcggcg gtgaccctgg gcacgctggc
     6061 ggagctcgcc gcacgctgag aactggtcga cgcggtggcc ggcgccgagt gacgccggcc
     6121 accgcctcca cgggtgatca cgcttcctgg aggaggccga tggggttgcc gtcggggtcc
     6181 ttgaccgagg cgatcagctt gccgccgccg acgtcgctga tcggctgctg ggcttccgcg
     6241 ccggcggtga gg
//
LOCUS       DASWDC010000072         3215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_424879, whole genome shotgun sequence.
ACCESSION   DASWDC010000072 DASWDC010000000
VERSION     DASWDC010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424879"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..909)
                     /locus_tag="VF995_02310"
     CDS             complement(196..909)
                     /locus_tag="VF995_02310"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9342436.1"
                     /translation="MLASGVSYRRLDVPGLDRLSGAGVFYGAAVAEAAAMAGQEVFVV
                     GAGNSAGQAALHLARHAAHVTILARGQSLAVSMSDYLVKEIGHTANLTVRLHTQVAAV
                     HGAGQLEGLTLQDISGGPPAEVPAAALFILIGLIGAQPHTDWLADTLTRDDRGFVLTG
                     RDLPRTPGPRTDGRWAAYRCCWNRACPGCSRPATCATGRSSGSRPPPATAPSPSSSST
                     STSANSDTALDREELDREE"
     gene            complement(948..1568)
                     /locus_tag="VF995_02315"
     CDS             complement(948..1568)
                     /locus_tag="VF995_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342437.1"
                     /translation="MRPWGHPEERPYPLIGELLSAWVKTTGRARLAAIRVVGRRRSPR
                     SHQLRNLLERNGVAYGFYFDDSAEGRQLLREAHQDGTRLPVVVMWDGRVLVDPSVAEI
                     VGAAGGETRPDPGVYDVAIVGAGPAGLAAATYGASEGLHTLQLEREAVGGQAGTSSLI
                     RNYLGFPRGSAAGSWPAGRPSRRGCSARASSSTSRPACTPAEPTGS"
     gene            2813..2947
                     /locus_tag="VF995_02320"
     CDS             2813..2947
                     /locus_tag="VF995_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342438.1"
                     /translation="MRPPRARLEGRLAAQHRRRLADVEAALAALDAVVHAVLDRDRDR
                     "
BASE COUNT          418 a         1111 c         1244 g          442 t
ORIGIN      
        1 gacccgctac caccccttga ggccgacctg tcggacctcc ctgggagaga cctgctgccg
       61 aacccacctg acgggggaag acggctcggc cgctgggtcg tcgggtgagg aacctctcga
      121 ggagatcgac gaggtcgccg aggtcgccga ggtcgccgcg gaagcatgtg gggaccagcg
      181 atccacaaga tttgcctact cctctcggtc cagttcctcc cggtccaggg ccgtgtcact
      241 gttcgctgag gtactcgtgg acgagctgga tggcgatgga gccgtcgccg gcggcggacg
      301 cgacccgctt gaccgaccgg tagcgcacgt cgccggccgc gaacaccccg ggcatgctcg
      361 attccagcaa caacggtagg cggcccagcg gccatcggtg cgggggcctg gagtcctggg
      421 caggtcgcgg ccggtgagga cgaacccgcg atcgtcacgt gtgagagtgt cggcgagcca
      481 gtcggtgtgc ggctgggcgc cgatcaggcc gatcaggatg aacagcgccg ccgccgggac
      541 ttctgcgggc ggcccgccgc tgatgtcttg gagcgtgagg ccctccagct gacccgcgcc
      601 gtggacggcg gcgacctggg tgtgcaggcg gacggtgagg ttggccgtgt ggccgatctc
      661 cttgaccagg taatcggaca tgctgaccgc cagtgactga ccccgggcca ggatcgtgac
      721 gtgggcggca tgcctggcca ggtgcagggc ggcctggccg gcggagttgc cggccccgac
      781 cacgaagacc tcctggcccg ccatggccgc ggcctccgcg acggccgcgc cgtagaacac
      841 cccagcgccg ctcaggcggt ccaggccggg gacatccagg cgccggtagg acacgccgct
      901 ggcaagcacg acggcccggc tggtcgcctg gctcccgtcg gccagggtca ggacccggtg
      961 ggctccgctg gtgtgcaggc cggtcgcgac gttgaagacg aagctcgcgc cgaacagcca
     1021 cgcctgctcg gacgccctgc tggccagctc ccggccgctg accctcgggg gaagccgagg
     1081 tagttgcgga tcagcgagct ggtgccggcc tggccgccga ccgcctcgcg ctccagctgc
     1141 agggtgtgta gcccctcgga cgcgccgtag gtggcggcgg ccaggccggc tgggccggcg
     1201 ccgacgatcg ccacgtcata gacgccggga tcgggtcggg tctcgccgcc ggcggcgccg
     1261 acgatctcgg ccaccgacgg gtcgaccagc acccggccat cccacatcac caccaccggc
     1321 agccgcgtcc catcctggtg ggcttcccga aggagctggc gtccctcggc ggagtcgtcg
     1381 aagtagaagc cgtaggcgac gccgttgcgc tccagcagat tcctgagctg gtgggagcgt
     1441 ggcgaccggc ggcgcccgac gaccctgatc gccgcgagcc gcgcacggcc ggttgtcttc
     1501 acccaggcgc tgagcagctc cccgatcagg gggtagggcc gctcctcggg atgcccccag
     1561 ggccgcacac tgtcgtcgtc gatctggccg agggggcatc gcctgcagga ccgctgcggc
     1621 cgcctggccg cccatgggga ccagcaggac gcgtttggcg gccgggtaca gttggtgggc
     1681 gcgggcgagg aacgccgcac cggtcagctg gggcggcgat tgggctgcga ccagcagagc
     1741 gacctggccg ccgcgtgcgc tcagccgctc caggaccgcc agccccccgg tgggcgagtt
     1801 ggcggtgtgg atctggtagt cggctgcgta gcgtcgctgc agggtttgct cgaggacgct
     1861 gagcgcctcg gggtcggcgt cgacgaccat caggatcggc ttggccaccg acgcacctcc
     1921 gcgggttggt cgggctgagc ggggcaggct cgtggccgac tggtcggcgc tgaccatggt
     1981 gcgatcagtg aagcaccaca gccagccctc gccgggctcc agcgagcggg tgatctggtg
     2041 gccgggcgcg cgctcggtgg cgtggcggtg ggctcggccg ctggtaggag ctcgcgacgc
     2101 gctcgctggc ccgcgggccg ggcggcacac ccacgtggcg aaccggggcg gatacccgcg
     2161 accattggga cggtcacctg ggacggtcac cgacggctca tgtgcgagcg ccgccgtgcg
     2221 ggtctgggcg cggcgtccct gctcgtggcg gtgttccggg cggggacagc ccggcccggc
     2281 cgccgacgtc ggcgcccggc cgccgcgtgt ccggcgggtc cccggctgac gcggcgatga
     2341 agagcgaccg gtcagcttgc cttgggcggc ggccagattg ccgtggccga gcaacagggg
     2401 cggtgggcag gcgttcgacg caggcgtaac gcgatgcgga gcgacgacgc tctttttgtt
     2461 tgatgagtgg tcccccagcg ctctcgtcgc gagcaaccga tcgtcgagca acggcaatgc
     2521 ccggccgccg gaccgtctgc tcgaccacct gcccatgccc gacctcaacc cgctgccgcc
     2581 gtgctacacg cggcgccgac cgtgacgtgg cccgcaggcc ggccgccggc cttggagggg
     2641 ttggattgga ggcgatgctc gatggaatcg ccgactggat cgccgctgct gtccggcgag
     2701 gccgcagggg acctcgcccg agtccgagcg gtgctgaacg gggttcgtgg cgagcggcag
     2761 caagcggcga tccaccacga gcaggcggcg gccggccacg ctgcccacgg cgatgcggcc
     2821 accgcgcgcg cggctcgagg gccgcttggc cgcccagcac cgccggcggc tggccgacgt
     2881 cgaggccgcc cttgcggcgc tggatgccgt cgtgcacgcg gtcctggacc gggaccggga
     2941 ccggtgagct gcgatgccgc gggatggcca ccgccgttcc gccgccctgc ggtggcggtt
     3001 gggcgcccgc gggcgcacgg ggccggacgg cccacatgca ggagctctga agacggcgtg
     3061 gggtgttccg ggtggggctg gagcaggcga cgttggcggg tgtgcccggc gcggctgttg
     3121 tccggctcgg ctcgtcgatc cgcgatgcgg cggccccaga cccggggtgg tgggtggtga
     3181 tggccaagcc tgccaacacc gccacgctcg gcggt
//
LOCUS       DASWDC010000073         5111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_174941, whole genome shotgun sequence.
ACCESSION   DASWDC010000073 DASWDC010000000
VERSION     DASWDC010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174941"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /gene="rho"
                     /locus_tag="VF995_02325"
     CDS             complement(<1..1680)
                     /gene="rho"
                     /locus_tag="VF995_02325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX9342439.1"
                     /translation="MATTERGILEAKVLPELQAIASSMGVQGYQRLRKADLIGAIIAK
                     AQGVEFVPSSAPASRGRRNSGTPAAAAPDENAGNAAGPPQSARGDGGDGAVAQEASTA
                     ARSFEAAPPAEGVAGNGGNGAAPPSQASQGESTQPAQSEAAAVPGPAQQQLVPAAVSA
                     PAPAQAHESDGQSQGEAPQAQQHQGEAAQGETRPATGADGQSDGQSGGQSGGQSGGGQ
                     SDGQAGGQTQGGGQRNEQQRDHSGGDRGPRRDWSQQQGGRWDNQQQGAGRRRNRNRNR
                     NRGGGGSGSGSGGGGGGGGNRFGNEQGNVALLERGADRGAAPDDRPEVARTGTLDIRS
                     QDGYGFLRVNGFLPGQDDVYVPLSMIRRLGLRRGDEVEGTVKLPRDSEKYAALQRVDK
                     VNGMDPEEARNRRDFKDLTPLYPQERLRLETEANQMTTRIIDLIAPIGKGQRGLIVSP
                     PKAGKTLVLQALANAISRNNPEVHLMVVLVDERPEEVTDMQRSVKGEVIASTFDRQAD
                     DHTIVAELAIERAKRLVELGHDVVVLLDSITRLGRAYNIAAPASGRILSGGV"
     gene            complement(2164..4386)
                     /locus_tag="VF995_02330"
     CDS             complement(2164..4386)
                     /locus_tag="VF995_02330"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX9342440.1"
                     /translation="MVDKVTSPQGEEFELADESVNRVLVKGVAAVLDQGTQRLWSTKE
                     GDMFLCADAHGNVDAEAAIGAGLYYKDTRFLSDYMLTVDGHVPLLLSTSADRAYASHV
                     DLANQDLVAVDGAIQAIQGTVNIRRTRVIKGRLYERIRVKNYNANPVELTLALRFFSD
                     FADIFEVRGLKRAQRGKFARPKADKAGAVLAYAGQDGVFRETRIGFGTPATTAEIHDE
                     QIVAVTWQLRLAPNETMVIPLTVEPRVGGEVPAQQSFDEAVHDLRRSYEEWERSCTRV
                     WTDNQLYNSVLTRAMRDLRALLTPTRHGKVVAAGIPWFVAPFGRDALLTCHQTLMLNP
                     DLTRTTLEVLAGFQADAVDKFRDAEPGKILHELRQGELAGAGIIPHSPYYGSIDSTSL
                     WLLLLGTYYRWTGDLDFCRQMLPHVERALSWVDTYGDLDGDGFLEYQSHSPRGLTNQG
                     WKDSHDSVVHLDGKLAEGPIALAEVQAYTYLAKLRVADVYEALGRSDQALVLRDQAGT
                     LRARFNERFWVEDEQFYAEALDGEKRPVASITSNPAHGLYCGIIDADRAALLARRLLA
                     PDMFSGWGIRTMSKSSVAYNPMSYHNGSVWPHDNAFIGAGLKRYGYIKATNRLATAVF
                     DMAVTVDDMRLPELFCGFTRRSPNRPVAYPVACSPQAWASGTPFLLLQSMLGISAQAP
                     QNTLTINQPLLPPWLNTVELHNLRVGRSTISIVFRREGEVTGFSLLEKEGNVRVLMEE
                     "
     gene            complement(4566..>5111)
                     /locus_tag="VF995_02335"
     CDS             complement(4566..>5111)
                     /locus_tag="VF995_02335"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX9342441.1"
                     /translation="DLLALASELEGHPDNVAPCLAGGATLCWGEAGRPRCRVLPIASE
                     LAALALVADQPMNTQEARRLLPERVPFGYAVHTAGRAALLPLALGGDLELLLPATEDV
                     LHQPRRLAVQPVAAHALGLLRARGHAAFLSGAGPSLLVLCPRDALHHTRDDAREALRD
                     ASSWRIEDLEISGGGATIELD"
BASE COUNT          707 a         1760 c         1845 g          799 t
ORIGIN      
        1 cacaccaccg gacaggatcc gcccgctggc cggggcggcg atgttgtacg cgcgcccgag
       61 ccgggtgatc gagtccagca acacgaccac gtcgtggccc agctcgacca ggcgcttggc
      121 ccgctcgatc gccagctccg cgacgatcgt gtggtcgtcg gcctgccggt cgaaggtcga
      181 ggcgatcacc tcacccttca ccgaccgctg catgtcggtg acctcttccg gacgctcgtc
      241 caccaggacg accatcaggt gcacctcggg gttgttgcgg ctgatcgcgt tcgccagcgc
      301 ctgcaggacc agggtcttgc cggccttcgg gggcgagacg atcagccctc gctggccctt
      361 gccgatcggg gcgatcagat cgatgatccg ggtggtcatc tggttcgcct cggtctccag
      421 gcgcagccgc tcctgcgggt acagcggggt caggtccttg aagtcgcggc ggttgcgcgc
      481 ctcctcgggg tccatgccgt tgaccttgtc gacgcgctgg agggcggcgt atttctcgga
      541 gtcgcgtggc agcttgacgg tgccttcgac ctcgtcgccg cgacgcaggc cgaggcggcg
      601 gatcatcgac agcggcacgt agacgtcgtc ctggccgggc aggaagccgt tgacccgcag
      661 gaagccgtag ccgtcctggg agcggatgtc gagcgtgccg gtgcgggcca cctcgggccg
      721 gtcgtccggc gccgcgccac ggtcggcgcc gcgctccagc agcgccacgt taccctgctc
      781 gttcccgaag cggttgccgc caccgccacc gccaccgccg ctgccgctgc cgctgccgcc
      841 gccgccgcgg ttgcggttcc ggttgcggtt gcggcgccgc ccggcgccct gctgctggtt
      901 gtcccagcgg ccgccctgct gttggctcca gtcccgccgg gggccgcggt cgccgccgct
      961 gtggtcgcgc tgctgctcgt tccgctggcc gccgccctgg gtctgcccgc cggcctggcc
     1021 atccgactgg ccaccgccgg actgcccgcc ggactgcccg ccggactgcc cgtcggactg
     1081 cccgtcggcg ccggtggcgg gacgggtctc accctgagct gcctctccct gatgttgctg
     1141 agcctggggc gcctcgccct ggctctggcc gtcgctctca tgggcctgag ccggtgcggg
     1201 cgcggacacc gccgccggga ccagctgctg ctgcgccggg ccgggaaccg ccgcggcctc
     1261 gctctgggcc ggctgggtgg attcgccctg ggaagcctgg gaaggtggcg cggcgccgtt
     1321 gccgccgttg ccggcgacac cctcggcggg aggggcagcc tcaaaggacc gcgccgccgt
     1381 ggaggcctcc tgggcaaccg cgccgtcacc cccatccccg cgagcgctct gcggcggccc
     1441 ggcggcgttg ccggcgttct cgtccggcgc agcggcggcg ggagtccccg agttgcggcg
     1501 gccccggctg gccggcgcgg aggaggggac gaactcgaca ccctgagcct tggcgatgat
     1561 cgcgccgatc aggtcggcct tgcgcagacg ctgatagccc tggacaccca ttgacgaagc
     1621 gatcgcctgc agctcaggca gcaccttggc ctcaaggatg ccgcgttcgg tggttgccat
     1681 cttcaccttc ctgtggtgat tccagcctga tcggcggact ctcgtcgcat tgccgaacga
     1741 agctttgtgt cggcgcctcc atccggcgcc agagatctcg tgcttgctcc tcttcccgtc
     1801 accaggatcc ggccgcccag cacgaagctc gcgccacatt gcgaaagctt cacactcagc
     1861 tgtgacctac cctcagccga ggtgtcggat caccaggatc cagttgatca atagaaagga
     1921 acgactggtc ttcgggtcgc tcgccaggct cgccggtcgc ccccggaagg ttgactgcaa
     1981 cgtcgatctg cactgcaagc cacaaaaatc accgaagtga tgtgccgggt ggcgggggcg
     2041 tcgaccgaat tggccagcga ctgagacctg aagcattgta cgagtgatca caaggccgat
     2101 gcaaccgtgc cttcgacgcc accgacggca gcgggcctgg gtgatgcccc ggcaggccgg
     2161 cgtctactcc tccatcagca cgcggacgtt gccctccttc tccagtaggg aaaaccccgt
     2221 gacctcgccc tcccggcgga acacgatcga gatcgtcgac cggcccaccc ggaggttgtg
     2281 cagctcgacc gtgttgagcc agggtggcag cagcggctgg ttgatggtga gggtgttctg
     2341 cggcgcctgc gccgagatcc cgagcatgga ctgtagcagc aggaacggcg tgccagaagc
     2401 ccatgcctgc ggcgagcagg ccaccgggta ggcgaccggc cggttcgggg agcggcgggt
     2461 gaaaccgcag aacagctcgg gcaggcgcat gtcgtcgacg gtcaccgcca tgtcgaacac
     2521 cgcggtggcc aggcggttgg tcgccttgat gtagccgtag cgcttcaggc ccgcgccgat
     2581 gaaggcgttg tcgtgcggcc acaccgagcc gttgtggtag ctcatcgggt tgtaggcgac
     2641 cgagctcttg gacatcgtgc ggatgcccca gccggagaac atgtcggggg ccagcagccg
     2701 ccgggccagc agcgccgccc ggtcggcgtc gatgatgccg cagtagagcc catgggcggg
     2761 gttggaggtg atcgacgcga ccgggcgctt ctccccgtcc agcgcctcgg cgtagaactg
     2821 ctcgtcctcg acccagaagc gctcgttgaa gcgcgcccgc agggtgccgg cctggtcgcg
     2881 cagcaccagc gcctggtcgg agcggcccag cgcctcgtac acgtcggcca cgcgaagctt
     2941 ggcgaggtag gtgtaggcct gcacctcggc cagggcgatc ggcccctcgg cgagcttgcc
     3001 gtccaggtgc accaccgagt cgtgggagtc cttccagccc tggttggtga ggccgcgcgg
     3061 gctgtggctc tggtactcga ggaagccgtc cccgtccagg tcgccgtagg tgtcgaccca
     3121 gctgagcgcc cgctcgacgt gcggcagcat ctggcggcag aagtccaggt cgccggtcca
     3181 gcggtagtag gtcccgagca gcagcagcca cagcgaggtc gagtcgatcg agccgtagta
     3241 ggggctgtgc gggatgatcc cggcgccggc cagctcgccc tggcgcagct cgtggaggat
     3301 cttgccgggc tcggcgtcgc ggaacttgtc gaccgcgtcg gcctggaagc cggccaggac
     3361 ctccagggtg gtgcgggtca ggtccgggtt gagcatcagg gtctggtggc aggtcagcag
     3421 cgcgtcgcgc ccgaacggcg ccacgaacca gggaatcccg gcggcgacca ccttgccgtg
     3481 gcgggtcggg gtgagcagcg cccgcagatc gcgcatggcc cgggtcagca ccgagttgta
     3541 gagctggttg tcggtccaga cgcgggtgca ggaccgctcc cactcctcat aggagcggcg
     3601 caggtcatgg acggcctcgt cgaagctctg ctgcgccggc acctccccgc cgacgcgggg
     3661 ctccaccgtc agcgggatca ccatggtctc gttcggggcg aggcgcagct gccaggtgac
     3721 ggcgacgatc tgctcatcgt gaatctcggc ggtggtggcg ggcgtgccga agccgatgcg
     3781 ggtctcgcgg aagacgccgt cctggccggc gtaggccagc accgcgccgg ccttgtcggc
     3841 cttgggccgg gcgaacttgc cacgctgggc gcgcttcagg ccgcgcacct cgaagatgtc
     3901 ggcgaagtcg ctgaaaaagc gcagcgccag ggtcagctcc accgggttgg cgttgtagtt
     3961 cttgacccgg atgcgctcgt agaggcgacc tttgatcact cgggtgcggc ggatgttgac
     4021 ggtgccctgg atcgcctgga tggccccgtc gaccgccacc aggtcctggt tggcgaggtc
     4081 gacgtggctg gcataggccc ggtcggcgga ggtcgacagc agcagcggca cgtggccgtc
     4141 gaccgtgagc atgtagtcgg agaggaaacg ggtgtccttg tagtagaggc cggcgccgat
     4201 ggccgcctcg gcgtcgacgt tgccgtgcgc gtcggcgcac aggaacatgt cgccttcctt
     4261 ggtggaccac agccgctgcg tcccctggtc caggacggcg gcgacaccct tgaccaggac
     4321 ccggttgacc gactcgtcgg ccagttcaaa ctcctcaccc tgcggcgagg tcaccttgtc
     4381 gaccacagaa acgactccgc atgcctcatg acccgtatcg gtctggattt tgtacctgcc
     4441 gcggcgccgg attccgcaac cggaactcgg cggtcccgat tcccgattcg cggatcccga
     4501 ttcgcggatc ccgattcccg gatcccgatt cgcgggaagg cgcggcgacg ggggcagtct
     4561 accggtcaat ccagctcgat cgtcgcaccc cccccagaga tctcgagatc ctcgatccgc
     4621 cagctggagg cgtcccggag tgcctcgcgg gcgtcgtccc gtgtgtggtg gagggcgtcc
     4681 cgggggcaga gcaccagcag ggaggggccc gcgccggaga ggaacgcggc gtgcccgcgg
     4741 gcccgcagca gcccaagggc gtgggccgcg accggctgga cggcgaggcg acggggctgg
     4801 tgcagcacgt cctcggtcgc gggcagcagc agctccaggt cgccgccgag ggcgagcggg
     4861 agcagggcgg cccggccggc ggtgtggacg gcgtagccga acggcacccg ctcggggagc
     4921 aggcggcggg cctcctgggt gttcatcggc tgatcggcga ccagcgcgag cgcggccagc
     4981 tccgaggcga tcggcaggac ccggcagcgg gggcggccgg cctcccccca gcacagggtg
     5041 gcgccgcccg caaggcaggg cgcgacgttg tcgggatggc cctccagctc gctggccagc
     5101 gcgagcaggt c
//
LOCUS       DASWDC010000074         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1432941, whole genome shotgun sequence.
ACCESSION   DASWDC010000074 DASWDC010000000
VERSION     DASWDC010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432941"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..542)
                     /locus_tag="VF995_02340"
     CDS             complement(258..542)
                     /locus_tag="VF995_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342442.1"
                     /translation="MSSSASGFSMRLQAGERRDTATSLSAAQRATLTRIADKRDGVQH
                     CDLPAGDLVTLLRSYLVRFSTPDLVEATARGRQALEGDAPAQAPSASADA"
     gene            738..974
                     /locus_tag="VF995_02345"
     CDS             738..974
                     /locus_tag="VF995_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF397 domain-containing protein"
                     /protein_id="HEX9342443.1"
                     /translation="MPDPQNGIPAGELGDLQWRKSGRSSAQGNCVETAQLTGGGAAIR
                     NSRHPEGPALVFHADEIRAFVEGAKDGDFDYLLA"
     gene            1203..1697
                     /locus_tag="VF995_02350"
     CDS             1203..1697
                     /locus_tag="VF995_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9342444.1"
                     /translation="MRDVPEAFALHPSSTPHPEPRLRILIADDHLGVREAMACLLTLI
                     GFEIVAQAEDGAVAVALAKQVTPDLILMDLSMPVLNGLDATRLIHRALPGTPVVILSA
                     YDSAELRHAAQAAGAAAYLVKGLPVETLRTTLLRLLPTAPNAGPTTADIVAVRSGRDR
                     DQVG"
     gene            complement(1694..2416)
                     /locus_tag="VF995_02355"
     CDS             complement(1694..2416)
                     /locus_tag="VF995_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9342445.1"
                     /translation="MLGTMADNSPAGSGFERLNRELVECRRCPRLVAWREQVARERRA
                     AFRDQAYWGRPVPGFGDPAARLLVLGLAPAAHGGNRTGRIFTGDRSGDWLFASLYRTG
                     FANQPASVARDDGLALADCYITAPVKCAPPDNKPLPEERATCAPWLRQELALLDATLH
                     VVVALGGYAWDTALRHLGPVRPKPRFAHGAEAALPHGRVLLGSFHVSQQNTSTRKLTE
                     PMLDGVFLHAWELLGGRGPARD"
     gene            2514..2996
                     /gene="folB"
                     /locus_tag="VF995_02360"
     CDS             2514..2996
                     /gene="folB"
                     /locus_tag="VF995_02360"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX9342446.1"
                     /translation="MGDVVFIQGLEVDAVIGAYDWERQLRQRLVLDLELAADVRKAAA
                     SDAVADAVDYAAIAERVTEFVQASDTQLIETLAERVAELITAEFGVPWLRLELRKPRP
                     LSGRLVAGVRIERGERPADPPPSSDHALHLERLRTLAQAADLGPQDPPHTDPTTAAPR
                     "
     gene            2993..>3472
                     /locus_tag="VF995_02365"
     CDS             2993..>3472
                     /locus_tag="VF995_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9342447.1"
                     /translation="MNAASSSSAEQVEEALGRIEALDGRLGAFVTVLAEQARAEAAER
                     DAERAAGRVRGPLHGAPVAVKDLIDVAGVVTSAGSPKLAGNRAERDAPVVARLREAGA
                     VLIGKTRTHEFAYGVLTPGTVNPWDETRIAGGSSGGSAAAVAAGLVRFAVGTDTAGSI
                     "
BASE COUNT          536 a         1241 c         1217 g          478 t
ORIGIN      
        1 taccggattt agcgaacgta gcattaaggg tcaggagcat gccgggatgc cccgaacggc
       61 caggtaggtg aacacctgcg gataggcgaa acacgcgttc gattgccgat gtggaaccgg
      121 tccaacggac aacccctacg ccccgttgcc gagttgccga cagaggagtt tctataccct
      181 ttgcccggag gaagggaacg gccggtccaa gcttctagga cattctgtcc tggaagtcgc
      241 gctagctacg agacgcgcta ggcgtcggcg ctggcgctgg gggcttgcgc cggtgcgtcg
      301 ccctcgagcg cctgccggcc gcgtgcggtt gcttcgacca ggtcaggggt gctgaagcgg
      361 acgaggtagg agcgcagcag ggtcaccaga tcgccagctg ggaggtcgca gtgctggacg
      421 ccgtcgcgct tgtcggcgat gcgcgtcagg gttgcccgct gcgcggcgct gaggctggtt
      481 gcggtatcgc gccgctcgcc agcctgaagc cgcatcgaga agccggatgc agaggaggac
      541 atgatccgta ccaccgcgac ctttgccgtt ccgggtgacg cacgccgtgt gccttggcgc
      601 ctcttatgat ttaccatgcc aagtgcagat gcaactgcga atgcaatcgc cagacgtgaa
      661 gtgcagacgc taagtgcaga tcgggagcgt ccgccgcaga aggtcggcac tttgcaagac
      721 catcaaagag gacgaacatg cctgacccgc agaacggcat ccccgccggc gaactggggg
      781 atcttcagtg gcgcaagagc gggcgcagca gtgcccaagg caactgcgtc gagaccgccc
      841 agctcacggg tggtggcgct gccatccgca actcccggca cccggaaggc ccggccctgg
      901 tcttccacgc cgacgagatc cgcgccttcg tcgaaggcgc caaggacggc gacttcgact
      961 atctgctcgc gtagcccgtc ccagacgcag cccacgatcg gcaccgcccg cccgacgatc
     1021 gtgggcgtcg ttatttgaca gcacgatcga cgccaggcag tcatcatgtc ccgcgtggcc
     1081 cgggtcggct tgcgtcgctg cgcgatcttt gcgctggccg atcgttttgc gtccgaggcg
     1141 gttttcaggc taaagttagc cactcgcggg aggtgggtgg cgtgcgactg catgagcagg
     1201 atgtgaggga tgttccggaa gcgttcgccc tccatccatc ctcgacaccg caccccgagc
     1261 cccgtctccg catcctgatc gccgacgacc acctcggcgt cagggaagcg atggcctgcc
     1321 tgctgactct gatcggcttc gagatcgtcg cccaggccga ggacggcgcc gtcgccgtcg
     1381 ccctcgccaa gcaggtcacc cccgacctga tcctcatgga cctctccatg cccgtcctca
     1441 acggcctcga cgccacccgc ctgatccacc gcgccctccc cggcaccccc gtcgtcatcc
     1501 tcagcgccta cgactccgcc gagctccgcc acgccgccca agccgccggc gccgccgcct
     1561 acctcgtcaa gggcctcccc gtcgagaccc tccgaaccac cctcctccgc cttctgccaa
     1621 ccgcgccgaa tgctggcccc acgaccgccg acatcgtcgc cgtccgatcc ggtcgggatc
     1681 gcgaccaagt cggctaatcg cgggccggcc cccggccgcc aagcagctcc caggcgtgga
     1741 ggaacacgcc gtccagcatc ggctcggtga gcttcctggt cgaggtgttc tgctggctga
     1801 cgtggaagct gccgaggagg acccggccgt gggggagggc cgcctcggcg ccatgggcga
     1861 agcggggttt ggggcggacg gggccgaggt ggcgcagggc ggtgtcccag gcgtagccgc
     1921 cgagggcgac gaccacgtgg agggtggcgt cgaggagcgc cagctcctgc cgcagccagg
     1981 gagcgcaggt ggcgcgttcc tcggggaggg gcttgttgtc ggggggagcg cacttcaccg
     2041 gggcggtgat gtagcagtcg gcgagggcga ggccgtcgtc gcgggcgacc gaggccggct
     2101 ggttggcgaa cccggtgcgg tacaggctgg cgaacagcca gtcgccggag cggtcgccgg
     2161 tgaagatccg gccggtgcgg ttgcccccgt gggcggcggg ggccaggccg aggaccagca
     2221 gccgcgcggc cgggtcgccg aacccgggca ccgggcgccc ccagtaggcc tggtcgcgga
     2281 aggcggcgcg cctctcgcgc gcgacctgct cacgccaggc gaccaggcgc gggcagcggc
     2341 ggcactcgac cagctcgcgg ttgagccgct cgaatccgga tccggccgga gagttgtccg
     2401 ccatggtgcc gagcatgctc tcattgtcac cgggcggcgg accaccgtcg gcaaagccgt
     2461 cggcaaagcc gtcggcaaag ccgtgcgcag ggcgagcgaa aggggacaca ggcttgggcg
     2521 acgtcgtctt catccagggg cttgaggtcg acgcggtgat cggcgcctac gactgggagc
     2581 gccagctgcg ccagcggctg gtgctcgacc tggagctggc cgccgacgtg cgcaaggccg
     2641 ccgccagcga cgccgtcgcc gacgcggtcg actacgccgc catcgccgag cgggtcaccg
     2701 agttcgtcca ggcgtccgac acccagctca tcgaaaccct cgccgagcgg gtcgcggaac
     2761 tgatcacggc cgagttcggc gtcccctggc tgcgcctgga actgcggaaa ccccggccgc
     2821 tcagcggccg cctggtcgcc ggcgtgcgca tcgaacgcgg cgagcgcccc gccgaccccc
     2881 cgccgagcag cgaccacgcc cttcacctgg aacgcctccg gacgctcgcc caggccgccg
     2941 acctcggccc gcaggacccg ccccacaccg acccgaccac agcggctccc cgatgaacgc
     3001 cgcctccagc tccagcgccg aacaggtcga ggaggccctc gggcgcatcg aggcgctcga
     3061 cgggcggctc ggcgcgttcg tcaccgtgct cgccgagcag gcacgagccg aggcggcgga
     3121 gcgggacgcc gaacgggccg ccgggcgggt ccgggggccg ctgcacgggg cgccagtggc
     3181 ggtcaaggac ctgatcgacg tcgcgggcgt ggtcaccagc gccggatcgc cgaagctcgc
     3241 cggcaaccgg gcggaacgcg acgccccggt ggtggcacgg ctccgcgagg ccggcgccgt
     3301 gctcatcggc aagacccgca cccacgagtt cgcctacggc gtgctgaccc ccggtaccgt
     3361 caacccctgg gacgagaccc gcatcgccgg cggctccagc ggcggctcgg ccgcagcggt
     3421 cgccgccggc ctggtccgct tcgcggtcgg aaccgacacc gccggttcga tc
//
LOCUS       DASWDC010000075         3785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_683579, whole genome shotgun sequence.
ACCESSION   DASWDC010000075 DASWDC010000000
VERSION     DASWDC010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683579"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /gene="larC"
                     /locus_tag="VF995_02370"
     CDS             complement(<1..272)
                     /gene="larC"
                     /locus_tag="VF995_02370"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HEX9342448.1"
                     /translation="MTFAYFDCFAGIAGDMVLGALLDAGASEERLRAGLDALHPGVGP
                     IELEIAATERGGIGATRVVVRAPGPPVVRDWAALRTVLDAADLPESV"
     gene            complement(279..1037)
                     /gene="larB"
                     /locus_tag="VF995_02375"
     CDS             complement(279..1037)
                     /gene="larB"
                     /locus_tag="VF995_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX9342449.1"
                     /translation="MDEREIRQLVDAVAQGVLPADEAVRRLARLPFEDLGFARVDHHR
                     ALRGLGPEVVYGRGKTPEEVAAIVAALLEAGGAPVLVTRAGQGHADAVREIVPEARYH
                     PRSSLLVCRAAPPDPDVGLVAVVCAGTSDLPVAEEAWVTAEAFGCRVDTLVDVGVAGV
                     HRVLAETARLQAADVLVVVAGMEGALPSLVAGLVAAPVVAVPTSVGYGASFGGVAALL
                     GMLNSCAVGIGVVNIDNGFGAGALAARILAQRKR"
     gene            1149..1709
                     /locus_tag="VF995_02380"
     CDS             1149..1709
                     /locus_tag="VF995_02380"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEX9342450.1"
                     /translation="MVVPPLPVARLTLLLYVAFGFCGLLAVVVAALGLRLRGIRRSYA
                     QLLDGGDHGDLLTLVGRQIEAAGQLRGTLDLISRETAQLRQRLSTVIRTVGFTRYNAF
                     PGSGGELSFSAAFLDEAGNGMVLSSISGRSESRLYAKQIVNGRCGHGLSDEERAAIAM
                     AIDSSATTPAPAPDAGSSGPIARSCR"
     gene            1865..3133
                     /gene="serS"
                     /locus_tag="VF995_02385"
     CDS             1865..3133
                     /gene="serS"
                     /locus_tag="VF995_02385"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX9342451.1"
                     /translation="MLDLKLIRDDPETVRAALARRGGDAAAAVDRLLELDATRRALVT
                     EVDGLRAEQKRGGRDVASAPPGERARLLASLKDLAARLDQAEGRLRTVDTDLYDLLAR
                     VPNLPDPSVPPGGEDDAEELRQVGTVPALGFPVRDHLELGELLGAIDTVRAAKVSGAR
                     FGYLTGPGAMLEFSLVRFAMDLLAGAGFTPMVPPVLVRREAMFGTGFLPTDEQQIFRT
                     ADDDLYLAGTSEVPLVSYHADEILDPADLPRRYVGFSTCFRREAGSYGKDTRGIFRVH
                     QFDKVEMVSFVLPEASRDEHELLLAHEEEILQALEIPYRVVNIAAGDLGGSAAKKYDC
                     EAWIPSQETWRELTSTSNCTDYQARRIGCRVREATGNRPVHTLNGTAIAVGRTIIALL
                     ENHQQHDGSVHVPKALQPYFGTEVIAPASG"
     gene            3144..3230
                     /locus_tag="VF995_02390"
     tRNA            3144..3230
                     /locus_tag="VF995_02390"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3178..3180,aa:Ser,seq:tga)
     gene            3400..>3785
                     /locus_tag="VF995_02395"
     CDS             3400..>3785
                     /locus_tag="VF995_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter"
                     /protein_id="HEX9342452.1"
                     /translation="MTWFKESLQQHPELAIFLTLAIGFFVGRLRYKSLTLGSVTGTLL
                     AGVLVGQLHIEIPDLVKTVAFIAFLFALGYQVGPQFFAGLRKGGAKQLILALISCVIG
                     LAGAYAVAKALGFGPGWGAGLLAGGLT"
BASE COUNT          545 a         1450 c         1257 g          533 t
ORIGIN      
        1 accgattccg gcaggtcggc cgcgtccagg accgtccgca gcgccgccca gtcacgcacc
       61 accggcggcc ccggggcacg caccaccacc cgggtggcgc cgatgccgcc gcgctccgtc
      121 gcggcgatct ccagctcgat cggcccgacg ccgggatgga gggcgtcgag tccggcgcgc
      181 agccgctcct ccgacgcgcc cgcatccagc agcgcgccga gcaccatgtc accggcgatc
      241 ccagcgaagc agtcgaaata cgcgaaggtc ataagcattc agcgctttcg ctgcgccagg
      301 atccgggcgg cgagggcgcc ggcgccgaag ccgttgtcga tgttgaccac gccgatcccg
      361 accgcgcagg aattcagcat accgagcagc gcggcgacgc cgccgaagct ggccccgtac
      421 ccgacgctgg tcggcaccgc caccaccggc gcggcgacca gcccggcgac cagcgacggc
      481 agcgcgccct ccatcccagc gaccaccacc agcacgtccg cggcctggag ccgggcggtc
      541 tcggcgagga cccggtggac cccggccaca cccacatcga ccagggtgtc aacccggcag
      601 ccgaatgcct cggccgtgac ccaggcctcc tccgcgaccg gcaggtcgct ggtgccggcg
      661 cacaccacag cgaccaggcc cacgtcaggg tccggtggcg cggcgcggca caccagcagg
      721 ctggagcgcg gatgatagcg tgcctctggg acaatctccc ggaccgcgtc ggcgtggccc
      781 tgcccggccc tggtcaccag caccggagcg ccacccgctt cgagcagggc cgccacgatg
      841 gccgcgacct cctcgggggt cttgccgcgg ccatagacga cctcgggtcc cagcccgcgc
      901 agggcgcggt gatgatccac ccgcgcgaag ccgaggtcct cgaagggaag cctggcaaga
      961 cggcggaccg cctcatcggc tggcagcacc ccctgggcca cggcatccac aagctgccgg
     1021 atttcgcgtt cgtccaaagc catcgcattg tacaatcgcc ccggccctgc cctcggcagc
     1081 gcctctaatt agcaccattg ccgtcgcccc gttcgccgtt ccccgttgtg atctcgcagg
     1141 agctcatcgt ggtcgtccct ccgctgccgg tcgcgcgcct gaccctcctg ctgtatgtcg
     1201 cgttcgggtt ctgcggcctg ctggccgtcg tggtggccgc tctggggctg cgcctgcggg
     1261 ggatccgccg cagctacgcg cagctgctcg acggcggcga ccacggcgac ctcctcaccc
     1321 tggtgggccg ccagatcgag gccgccggcc agctccgcgg cacgctcgac ctgatcagcc
     1381 gcgagaccgc ccagctgcgc cagcgcctct cgaccgtgat ccggacggtc ggcttcaccc
     1441 gctacaacgc cttccccggc agcggcgggg aactgtcgtt ctcggccgcc ttcctggacg
     1501 aggcgggcaa cggcatggtg ctctccagca tcagcggccg ctcggagagc cgcctgtacg
     1561 ccaagcagat cgtcaacggc cgctgcgggc acggcctctc cgacgaggag cgtgccgcga
     1621 tcgccatggc gatcgacagc agcgccacga cccctgcccc ggcccctgat gcgggcagtt
     1681 cgggtccgat cgcccgatct tgtcgctgac cggccgctgc ccgagtatgc aagcgagcga
     1741 agcgatgccg tccatgggac ggccgccgga ggcggagctt ggcgggattg gccggcaggc
     1801 attggcgcat cctgcccacg gactcgttac gtttagtgtg gattcgctga atctggaggc
     1861 aaccgtgctc gatctcaagc tcatccgtga cgacccggaa acggtccgcg ccgctctggc
     1921 ccgccggggc ggtgacgccg ccgcggcggt cgaccgcttg ctggagctgg acgccacccg
     1981 ccgcgccctg gtcaccgagg tcgacggcct gcgcgccgag cagaagcgcg gcggcaggga
     2041 cgtcgccagc gccccacccg gggagcgtgc ccggctgctc gccagcctga aggacctcgc
     2101 cgcccgcctc gaccaggccg agggccggct gcgcaccgtc gacaccgacc tctacgacct
     2161 gctcgcgcgg gtcccgaacc tgcctgaccc gtcggtgccg ccaggtggcg aggacgacgc
     2221 cgaggagttg cgccaggtcg gcaccgtccc cgcgctcggc ttccccgtcc gcgaccacct
     2281 ggagctgggc gagctgctgg gcgcgatcga caccgtgcgc gccgccaagg tctcgggcgc
     2341 ccgtttcggc tacctcaccg gccccggggc gatgctcgag ttctcgctgg tccgcttcgc
     2401 gatggacctg ctcgccgggg ccggcttcac cccgatggtt cccccggtgc tggtgcggcg
     2461 cgaggcgatg ttcggcaccg ggttcctgcc aaccgacgag cagcagatct tccgtaccgc
     2521 cgacgacgac ctgtacctcg ccggcacctc ggaggtgccg ctggtcagct accacgccga
     2581 cgagatcctc gaccccgctg acctgccgcg ccgctacgtc ggcttctcga cctgcttccg
     2641 ccgcgaggcc gggtcctacg gcaaggacac ccgcgggatc ttccgggtgc accagttcga
     2701 caaggtcgag atggtctcgt tcgtgctgcc ggaggcgtcg cgggacgagc acgagctgct
     2761 gctcgcccac gaggaggaga tcctgcaggc cctggagatc ccctaccggg tggtcaacat
     2821 cgccgccggc gacctcggcg gctcggccgc caagaagtac gactgcgagg cctggatccc
     2881 ctcgcaggag acctggcggg aactgacctc gacctccaac tgcaccgact accaggcccg
     2941 ccgcatcggc tgccgggtcc gcgaggcaac cggcaaccgc ccggtgcaca ccctcaacgg
     3001 caccgcgatc gccgtcggcc gcaccatcat cgccctcctg gagaaccacc agcagcacga
     3061 cggctcggtc cacgtcccca aggccctgca gccctacttc ggcaccgaag tgatcgcccc
     3121 cgcctccggg tagactcacc tgcggaggag tggccgagcg gccgaaggca gcgaccttga
     3181 aagtcgccgg gcccgggagg gtcccgtgag ttcgaatctc acctcctccg ctggaacgcc
     3241 ggccgaaaga tcggcgtcga cgcgatcacc tcaagccggg atactcgacc ggcgttccag
     3301 ctccctggcc cctgccagcc ccgtttcggt caagccgccc ggctgggcgc ccgacaccta
     3361 accacatggt cgtgcgggac gggcgaaggg gcggaagccg tgacgtggtt caaggagtcg
     3421 ctgcagcagc accctgagct ggcgatcttc ttgacgctgg cgatcgggtt cttcgtcggc
     3481 cggttgcgct acaagtcgct gacgctcggc tcggtgaccg ggacactgct ggccggcgtg
     3541 ctggtcgggc agctccacat cgagatcccg gacctggtca aaacggtcgc gttcatcgcc
     3601 ttcctgttcg ccctcggcta ccaggtcggg ccgcagttct tcgccgggct gcgcaagggc
     3661 ggcgccaagc agctcatcct cgcgctcatc tcctgcgtca tcggcctggc cggcgcgtac
     3721 gcggtcgcca aggccctcgg cttcgggccc ggctggggcg cggggctgct ggcgggcggc
     3781 ctcac
//
LOCUS       DASWDC010000076         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1601452, whole genome shotgun sequence.
ACCESSION   DASWDC010000076 DASWDC010000000
VERSION     DASWDC010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601452"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VF995_02400"
     CDS             <1..954
                     /locus_tag="VF995_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9342453.1"
                     /translation="PNLTASSLRRADRKTSAIGLLLDDVANPFSSALHRAVEEVARGR
                     GTLVFAGSSDEDPDRERELLLALVARRVDGLIAFPVPAGARNPDARNPDAPGGAPGGA
                     FGGAGGGRARLLSESRLGRPMVVVDRPLPTGDVDSVTVDNRDSSRRAIGHLASHGHRR
                     IAFLGDLRTIWTAAERYIGYLEGLAASGLPLDPRLVRHDLRSIDDAERATVELLGAAP
                     PATRPSALFTGRNLITIGAVRALQRLGLHQQVALVGFDDILLGDLLAPRISVIAQDHA
                     AIGRTAAELLFGRLDGDHGPARQLVVPTRLIPRGSGELPAR"
     gene            complement(1000..1965)
                     /locus_tag="VF995_02405"
     CDS             complement(1000..1965)
                     /locus_tag="VF995_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018836697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEX9342454.1"
                     /translation="MVDVLTPIGPAELVRLAAVARRFYIDGRSKLEIAAEFELSRFKV
                     ARLLDQALTHGIVRIEIGVPAQIDAALSERIRAAYQLRHAIVVDTPEHPEADLRAHLA
                     RVAADLLAETVVEGDILGLGYGRTLTVMTEALTRIARCTVVQLSGALLGVNVQENTIE
                     LVRRVATVSGGPAFPIYTPQVLPDFATAEALRREPQVAEAQRRWNQLTRAIVAVGSWD
                     PPHSQLYVALSQRERDRLRAQGACAELCANLLDADGQPVATDFTDRCITITAAQLRAI
                     DEVVAVGGGADKVAAFKAVLRGGYATSLITDAIVARALLEGVRPR"
     gene            2106..>2791
                     /locus_tag="VF995_02410"
     CDS             2106..>2791
                     /locus_tag="VF995_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9342455.1"
                     /translation="MRDRALLLVLALALAAGACSGAGGGGGGGSQGGKTTITVATVAN
                     PQMQDIQKLSSAFEKDNPNINVKFVILPENELRDRVTQDIATKGGQYDVVTIGTFETP
                     QWAKNGWIENLGPYTQKDPSYDAGDLIPTVAKALSYNGSLYAVPFYGESSFLMYRKDL
                     FQQAGLTMPAQPTWDQVAQFAAKLNNPKKNQAGICLRGLPGWGELFAPLNTVVNTFGG
                     RWYDMNWNAQL"
BASE COUNT          413 a         1022 c          978 g          378 t
ORIGIN      
        1 cccaacctga ccgccagcag cctgcgccgc gccgaccgca agacctccgc catcggcctg
       61 ctgctcgacg acgtcgccaa cccgttctcc tcggcgctgc accgcgccgt cgaagaggtc
      121 gcgcgcgggc gcggcaccct ggtgttcgcc ggcagcagcg acgaggaccc cgaccgcgaa
      181 cgggagctgc tgctcgccct ggtggcgcgg cgggtcgacg ggctcatcgc cttcccggtc
      241 cctgccggcg cccgcaaccc cgacgcccgc aaccccgacg cccccggcgg cgcccccggc
      301 ggcgcctttg gcggtgcggg cggtggccgc gccaggctgc tcagcgagtc gcggctcggc
      361 cgtccgatgg tggtcgtcga ccggccgctg cccaccggcg acgtcgacag cgtcaccgtc
      421 gacaaccgcg acagctcccg gcgcgccatc ggccacctgg ccagccacgg ccaccgccgg
      481 atcgccttcc tgggcgacct ccgcaccatc tggaccgcgg cggagcgcta tatcggctac
      541 ctggagggtc tcgcggccag cggcctcccg ctcgacccgc gcctggtccg ccatgacctg
      601 cggagcatcg acgacgccga gcgcgccacc gtggagctgc tcggcgccgc acccccggcc
      661 acccggccct cggcgctgtt caccggccgg aacctgatca ccatcggggc cgtgcgtgcg
      721 ctccagcgcc tcggcctcca ccagcaggtc gcgctcgtcg gcttcgacga catcctcctc
      781 ggggacctgc tcgccccgcg catctcggtg atcgcccagg accacgcggc gatcggccgc
      841 accgccgccg agctgctgtt cggccgcctc gacggcgacc acggcccagc ccggcagctg
      901 gtcgtcccga cccgcctgat cccccgcggc tccggcgagc tcccagcccg gtgacgtcgc
      961 cgcggatgtc gaggccgagc tcgacaacga tcgccggggt cagcgcggcc ggacgccctc
     1021 cagcagcgcg cgggcgacga tcgcgtcggt gatcaggctg gtggcgtagc cgccgcgcag
     1081 gaccgccttg aaggcggcga ccttgtcggc gccgccgccg accgcgacca cctcatcgat
     1141 ggcgcgcagc tgcgcggcgg tgatggtgat gcagcggtcg gtgaagtcgg tggcaaccgg
     1201 ctggccgtcg gcgtcgagca ggttggcgca cagctccgcg cacgcgccct gggcccgcag
     1261 ccggtcgcgt tcgcgctggc tcagcgcgac gtagagctgc gagtgcggcg ggtcccagga
     1321 gccgacggcg acgatcgccc gggtgagctg gttccagcgc cgctgcgcct cggcgacctg
     1381 cggctcgcgc cgcagcgcct cggcggtggc gaagtcgggc aggacctggg gggtgtagat
     1441 cgggaacgct gggccgccgg agaccgtggc gacccggcgg accagctcga tggtgttctc
     1501 ctggacgttg acgccgagca gggcgccgga cagctgcacc acggtgcagc gggcgatgcg
     1561 ggtgagcgcc tcggtcatga cagtcagggt gcgaccgtag ccgaggccga ggatgtcgcc
     1621 ctcgaccacc gtctcggcga gcaggtcggc ggcgacccgg gcgaggtggg cgcgcaggtc
     1681 ggcctcgggg tgctcggggg tgtccaccac gatcgcgtgg cgcagctggt aggcggcccg
     1741 gatccgctcc gacagcgcgg cgtcgatctg ggcgggcacg ccgatctcga tgcggacgat
     1801 gccatgggtg agggcctggt cgagcagccg cgcgaccttg aaccggctga gctcgaactc
     1861 cgccgcgatc tcgagcttgg agcggccgtc gatgtagaag cggcgcgcga cggcggccag
     1921 gcggaccagc tcggcggggc cgatcggagt caggacatcc accatgcagg aacctcgcac
     1981 aagtgagcga tgcttcgctc attctttcat agggctcttg acaggccgaa taccctcgcc
     2041 ttcaatgaat tttagcaaac gagctataga gtgctcaaat gagcacccgc ctgggaggtg
     2101 ggtcgatgcg tgatcgcgcg ctcctgctcg tcctggccct ggccctggcc gccggcgcct
     2161 gcagcggcgc tggcggtggt ggcggtggtg gctcccaggg ggggaagacc accatcaccg
     2221 tcgccacggt cgccaacccg cagatgcagg atatccagaa gctgtccagc gccttcgaga
     2281 aggacaaccc gaacatcaac gtcaagttcg tgatcctgcc ggagaacgag ctgcgcgacc
     2341 gggtgaccca ggacatcgcc accaagggcg ggcagtatga cgtggtgacg atcgggacct
     2401 tcgaaacgcc gcagtgggcc aagaacggct ggatcgagaa cctcgggccg tacacccaga
     2461 aggaccccag ctatgacgcc ggcgacctga tcccgacggt cgccaaggcg ctgtcctaca
     2521 acggcagcct ctacgcggtg ccgttctacg gcgagtcctc gttcctgatg taccgcaagg
     2581 acctgttcca gcaggcgggg ctgacgatgc ccgcgcagcc cacctgggac caggtcgccc
     2641 agttcgccgc caagctcaac aacccgaaga agaaccaggc tggcatctgc ctgcgcggcc
     2701 tgcccggctg gggcgagctg ttcgcgccgc tcaacaccgt ggtcaacacc ttcgggggcc
     2761 gctggtacga catgaactgg aacgcacagc t
//
LOCUS       DASWDC010000077         8505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1184766, whole genome shotgun sequence.
ACCESSION   DASWDC010000077 DASWDC010000000
VERSION     DASWDC010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184766"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF995_02415"
     CDS             <1..237
                     /locus_tag="VF995_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX9342456.1"
                     /translation="STPYVTFYEGTAIGIELPAAVELEVRETEPGLKGDRVSGATKPA
                     TVETGAVVQVPLFVEVGDKIKVDTRSGSYLTRVQ"
     gene            234..680
                     /gene="nusB"
                     /locus_tag="VF995_02420"
     CDS             234..680
                     /gene="nusB"
                     /locus_tag="VF995_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX9342457.1"
                     /translation="MSDDARSPPPPGSRTAARRRALDVLYAADLLDQPIAKILADALH
                     EPDDSRPTPYAVELASGVERYRSELDTRIEAAAERWTLDRMPLVDRNVLRLATYELLA
                     EPTLATAVILNEAVELAKLLSTADSGRFVNGVLSRIARETRPEPAR"
     gene            complement(734..1951)
                     /locus_tag="VF995_02425"
     CDS             complement(734..1951)
                     /locus_tag="VF995_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX9342458.1"
                     /translation="MAGAARRISVEERRARLAWRHHLAAEARSDDVVGVVRDLVGLHA
                     SDPASVFLAVATRTRPAAAAAAVQRALYDDRALVRMLAMRRTMFVVPTELAPVVQAGV
                     TRAIAALERRRFVQLLDQAGVAGGADAGSWLQEVEASTLRALLARGEATAAELVADEP
                     RLRSQITLFADRAYGGAQNITTRVLLLLAADGRIVRGRPRGSWTSTQNRWAPAEAWFA
                     DPGALADLPIEAAQTELARRWLAAFGPAPLADLRWWTGWTAAETKRALKAVAPAEVDL
                     DGAPGLALPDDLEPVPSPAESWVALLPALDPTVMGWSGPGRSWFLGEHAPALFDRSGN
                     AGPTVWYNGRVIGGWAQRKTGAIAYRLLEDPGADASAAVDAAADRLQTWIGEVRLAPR
                     ARVPTALERELSA"
     gene            complement(1969..2331)
                     /locus_tag="VF995_02430"
     CDS             complement(1969..2331)
                     /locus_tag="VF995_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342459.1"
                     /translation="MCTAVRVVVAAADHARRLELRRAVVTAEWEVVGEADSPEAAYRE
                     AVERRARFLVLDATAAGSRPEGIVRRLKSVSPNAFVVTVGDLAAAGADAAVPADGLDA
                     LRDTMRDLLHASGDHAHR"
     gene            2408..3244
                     /locus_tag="VF995_02435"
     CDS             2408..3244
                     /locus_tag="VF995_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX9342460.1"
                     /translation="MPHGPGRLAPAFAPAAVLVVSALLTAACGGSGGARPAATTTSPT
                     PGTRAGAGGTLRVLVFSKTAGFRHASIPAGIAAIQELGRGNGFAVDATEDATRISDAG
                     LAPYRVLVFLSTTGDFLDSAQQAALQRWVERGGGWVGIHAAADAEYHWAFYGELVGAW
                     FARHPKVQRATIHVTDRRHPATAALPETWTRTDEWYDFKTNPRGAVHVLATVAESSYQ
                     GGSMGADHPIIWCHPVGAGRSFYTALGHTTESFAEPLYLAQLLGAIRWAGGQAAGDCG
                     SG"
     gene            3519..4271
                     /locus_tag="VF995_02440"
     CDS             3519..4271
                     /locus_tag="VF995_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342461.1"
                     /translation="MSRLDEDDAAIGDMVGRIAKTQERHGRKLNDVRTRVGAIEENHG
                     KKLDGLGRRLDGLAATVNAIEEDHGAKLDHLGTRVGTVEGKLETLQGSVDGVRMRVAA
                     VDLKMGLQISRIDRMQGTLDDQSSVIADHGQNIAALHSAIRDQDGRLGGVEDRLAGVE
                     GRMGAVEGSVREQGGRLVAVEVRLGGVEGRLGGVEGRLGGVEGAVREQGERLGGVQET
                     LREHSDHFGGIQGTLRMILDRLPERGNGGDGS"
     gene            4351..5001
                     /locus_tag="VF995_02445"
     CDS             4351..5001
                     /locus_tag="VF995_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9342462.1"
                     /translation="MTTTSSAQPVPSPRRVRARRGEGERLRDEILAAAARLLIETGDE
                     SAVSIRAVAAAVGVTPPSIYLHFGDKNELLRAVCSEQFRQLAERNEAAAASTDDPVEA
                     LRRCGRAYVAFGLENPEAYRILLMCHDREGPERAQPEAVANAAAFFDLVQRVQRAIDA
                     GALGPVDPVQAALGLWATAHGLTSLLIAHPAFPWPQDRAQLIDRILDQALTGLLAG"
     gene            complement(5019..5300)
                     /locus_tag="VF995_02450"
     CDS             complement(5019..5300)
                     /locus_tag="VF995_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342463.1"
                     /translation="MIVTASALCAAAGRDCGTLQEVVVLAWSEGAISYAVAVNNPSGL
                     APDKSSAGTAWDAELLRVASGLACPANSRRLVAAHEVVFPDGVRQPSPC"
     gene            complement(5308..6900)
                     /locus_tag="VF995_02455"
     CDS             complement(5308..6900)
                     /locus_tag="VF995_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEX9342464.1"
                     /translation="MTDEEQAAFSAFVRARSPALLRTAYLLTGDAMLAEDLLQTALVK
                     TLLAWERIRDRELVDAYVRRVMVNTQVTWWRRRWRLEQPADSLADRPVADGCAEVDER
                     DRLRRVLARLPVRQRTVVVLRYFDDLSEADTAALLGCSVGTVKSQSARALAKLRVALA
                     LDGHPWRTPAAVRAGHLRPVAERDDPATATEDSNRGGGRPAVRLTEGELREGLQALGE
                     QVDESPHRLSTEERLRRVLEQCRACEHASQWPGRDGRTWTRPHAGTRPPAGARRPRWP
                     RRRGLLARARERCAVAPVGAVALLVALAAGLAVVQGELRDGHWTVLTPKAAGGADANV
                     MVLDPKRGDSMTTATTARIELPRSASAVSCKLYQEVDGAPCGLLGDWLQPTSDPAILP
                     PGAHLMVALRGKLGWQAPATLGGSVLRRDFAEVFGDGTTAAGFTAVAEGATAVGAAVV
                     RKDGSSSASARWWDVKVTTNPLLTGAFQVPEPVAAPSLTPRYRQPTYLPAGARLAVKG
                     VELAAHGFVVRYTLPGPGRPAA"
     gene            7232..8395
                     /locus_tag="VF995_02460"
     CDS             7232..8395
                     /locus_tag="VF995_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342465.1"
                     /translation="MATPRNRTASDHNSGHALNAAISQLYIRCAVNGLDPDQIQAEIR
                     RAHPRIHPLEARRRAINWSRQQFMDAIAVVLWERDGVPSPLRDPDVFEWERKGRVPLQ
                     YIDVICRVLRCSAADIGFPEYSADHRPRLQPQDAQNQEIRTDLRYAAGESALLDAVAQ
                     GASIAYIGMLHSPGIDAEQYVVRVDGRCVLVDRRTFVQLAGAIPGFAFLGTIDFGLQP
                     DGRARIAGALTGAGSVDLSLVADLRQSRKSSAKLLEAEQLLDKADPSDTPAFIYHFKP
                     AWELPVIRGHCFRYLGRWEEAANAQTECLGAYPADRIRDRGVAHARRSLASLLDGHAD
                     AACAEAQLALPIALETASARILNDLRTLRATALRRWPEYPSVRDLNDLLSPVA"
BASE COUNT         1282 a         3201 c         2931 g         1091 t
ORIGIN      
        1 tccaccccct acgtgacctt ctacgagggc accgccatcg gcatcgagct ccccgccgcc
       61 gtcgagcttg aggtccgcga aaccgagccc ggcctcaagg gcgaccgcgt ctccggcgcg
      121 accaaacccg ccaccgtcga aaccggcgcc gtcgtccagg tccccctgtt cgtcgaggtc
      181 ggcgacaaga tcaaggtcga cacccgctcc ggctcctacc tcacccgagt ccagtgagcg
      241 acgacgcccg ctcgccgccg ccccccgggt cgcgcacggc cgcccgccgc cgcgccctcg
      301 acgtcctcta tgccgccgac ctgctcgacc agccgatcgc caagatcctc gccgacgccc
      361 tccacgagcc cgacgacagc cgtcccaccc cctacgccgt cgagctcgcc agcggcgtcg
      421 agcgctaccg cagcgaactc gacacccgca tcgaggccgc cgccgagcgc tggaccctcg
      481 accgcatgcc cctggtcgac cgcaacgtcc tgcgcctcgc cacctacgag ctcctcgccg
      541 aacccaccct cgccaccgcc gtgatcctca acgaggccgt cgaactcgcc aagcttctct
      601 ccaccgccga ctccggccgc ttcgtcaacg gcgtcctcag ccgcatcgcc cgcgaaaccc
      661 gccccgaacc ggcacgctag ccttcgcttg agaggacgtc ttcgagcagg ggtcgggctg
      721 agcggaagtc aggtcaggcg gagagttccc gctccagcgc ggtggggacg cgggcgcgcg
      781 gggcgaggcg gacctcgccg atccaggtct ggaggcggtc agccgccgcg tcgacagcgg
      841 cgctggcgtc ggcaccgggg tcttcgagga ggcggtaggc gatcgcaccg gtcttgcgtt
      901 gggcccagcc gccgatgacg cggccgttgt accagaccgt ggggccggcg ttgccggagc
      961 ggtcgaagag ggcaggggca tgctcgccga ggaaccagga gcggcccggg ccggaccagc
     1021 ccatcacggt ggggtcgagg gcggggagca gcgccaccca cgactcggcg ggggagggga
     1081 ccggctccag gtcgtcgggg agggccagcc ccggggcgcc gtccaggtcg acctcggcgg
     1141 gggcgacggc cttgagcgca cgcttggtct cggcggcggt ccagccggtc caccagcgca
     1201 ggtcggcgag gggggcgggg ccgaaggcgg cgagccagcg ccgcgccagc tcggtctgag
     1261 ccgcctcgat gggcaggtcg gcgagggcgc cgggatccgc gaaccacgcc tcggccgggg
     1321 cccagcggtt ctgggtgctg gtccaggagc cgcgcggccg gccgcggacg atgcgcccgt
     1381 cggcggcgag cagcagcagc acccgcgtgg tgatgttctg cgcgccgcca taggccctgt
     1441 ccgcgaacag cgtgatctgg gagcgcaggc ggggctcgtc ggcgaccagc tccgccgcgg
     1501 tcgcctcgcc ccgagccagc agcgcccgca gggtcgacgc ctcgacctcc tggagccagc
     1561 tccccgcgtc ggcgccgccg gcgactccgg cctggtcgag cagctggacg aagcggcgcc
     1621 gctccagcgc cgcgatcgcc cgggtcacgc ccgcctgcac caccggcgcg agctcggtcg
     1681 gcacgacgaa catcgtgcgg cgcatggcga gcatccgcac cagcgcccga tcgtcataca
     1741 gcgcccgctg gaccgccgca gccgccgccg ccgggcgggt ccgcgtcgcc accgccagga
     1801 acaccgacgc cgggtcgctc gcgtgcagcc cgaccaggtc ccgcaccacc ccgaccacgt
     1861 cgtcgctgcg ggcttcggcg gcgagatggt ggcgccaggc gagccgtgcg cgccgctcct
     1921 cgacgctgat ccgccgtgcc gctcccgcca tggccgtggc ccccgggctc agcggtgggc
     1981 gtgatcgccg ctggcgtgga gcaggtcccg catggtgtcg cggagggcgt cgaggccgtc
     2041 ggcggggacg gccgcgtccg cgcccgccgc tgccaggtcg ccgacggtga ccacgaaggc
     2101 gttggggctg accgacttga ggcgccgcac gatcccctcg ggccgggagc cggcggcggt
     2161 cgcgtccagg accaggaagc gggcgcggcg ctcgaccgcc tcgcggtagg cggcctctgg
     2221 gctgtccgcc tcgccgacca cttcccactc ggcggtcacc accgcgcggc gcagctccag
     2281 gcgccgggcg tggtcggcgg cggcgacgac gacgcggacg gcggtgcaca cagcggcaac
     2341 tccccccaga tcagcagacc tgtcgacgag aacacaacga cgcgaagact cgtatcctac
     2401 cggtgcgatg ccacatggac cgggccgcct ggcgcctgcc ttcgcgcccg ccgccgtgct
     2461 cgtggtcagc gcgctgctga ccgccgcctg cggcgggtcg ggcggcgccc ggccggcggc
     2521 caccacgacc agcccgacgc ccgggacgcg ggccggggcc ggcgggacgc tgcgagtgct
     2581 ggtgttctcc aagaccgccg ggttccgcca cgcctccatc ccggcgggga tcgccgccat
     2641 ccaggagctc ggccgcggca acgggttcgc cgtcgacgcc accgaggacg ccacccggat
     2701 cagcgacgcc ggcctcgccc cctatcgggt gctggtgttc ctgtcgacca ccggcgactt
     2761 cctcgacagc gcccagcagg ccgcgctgca gcgctgggtc gagcgcggcg gcggctgggt
     2821 cggcatccac gccgctgccg acgccgagta ccactgggcc ttctacgggg aactggtcgg
     2881 ggcgtggttc gcgcgccacc cgaaggtgca gcgagccacc atccacgtca ccgaccgccg
     2941 ccacccggcc accgccgcgc ttccggagac ctggacccgg accgacgagt ggtacgactt
     3001 caagaccaac ccgcgcgggg ccgtccacgt cctggccacc gtggcggagt cgtcgtacca
     3061 gggcggctcg atgggtgccg accatccgat catctggtgt catccggtgg gcgccgggcg
     3121 ctccttctac accgccctcg gccacaccac cgagagcttc gccgagcccc tctacctggc
     3181 gcagctcctc ggcgcgatcc gctgggcggg cgggcaggcc gccggggact gcggctccgg
     3241 atagcggctt gaccaccact gccgaaaccg atgcaaacaa tcgctgaaac aagtgttcga
     3301 cagtgaacgt atgttcgact atactgacgc gaccgaagca accatcccga agggaacggg
     3361 gtggcgtcac agggtcccgg gacggagaat ccgggtccaa cccgaacgga cgccggctgg
     3421 cggtgcgacc ccgccgtcac cggatcacag cgaggacgcg gggggcagca atgagcgaga
     3481 tgactatcgg agagcaggtc tcgcggctca cggccagggt gagccgcctg gacgaggacg
     3541 acgccgccat cggggacatg gtcggcagga tcgcgaagac gcaggagcgg cacggcagga
     3601 agctgaacga cgtcaggacc agggtcggcg cgatcgagga gaaccacggc aagaagctgg
     3661 acggcctcgg caggcgcctg gacggtctcg cggccaccgt caacgcgatc gaggaggacc
     3721 atggcgcgaa gctggaccac ctcggcaccc gggtcggcac ggtcgaaggc aagcttgaaa
     3781 ccctccaggg gagtgtcgac ggcgtccgga tgcgagtcgc cgcggtggat ctcaagatgg
     3841 gcctgcagat cagccgcatc gaccggatgc aaggaaccct ggacgatcag tcctcggtga
     3901 tcgccgacca cggtcaaaac atcgcggcac tccacagcgc gatccgtgac caggacggcc
     3961 gtctgggcgg cgtcgaggat cgtctggccg gggtcgaagg acgcatggga gcagtcgaag
     4021 gctcagtgcg agaacaaggt ggccgcctgg tagcggtcga agtgcgcctc ggtggcgtcg
     4081 aaggacgcct tggtggcgtc gaaggacgcc ttggtggcgt tgaaggggcc gtgcgggagc
     4141 agggcgagcg ccttgggggc gttcaagaga cgcttcgtga gcacagcgac catttcggag
     4201 ggatccaggg gacgctgcgg atgatcctcg accggcttcc tgagcgtgga aacggaggcg
     4261 atggctcctg acgggaacct caagggtagg ctgaagcgtc cgatgacacg aagagactag
     4321 accgctttcg ccggggacga ccaagttcgg atgacaacca cctcatcagc acaacctgtt
     4381 ccttctcctc gtcgggtccg cgcccggcgg ggagaagggg agcggctgcg tgatgagatc
     4441 ctcgccgccg ccgcccggct gctgatcgag accggggacg agtcggcggt gtccatcaga
     4501 gcggtcgccg ccgcggtggg ggtcacgccg ccctcgatct acctgcattt cggggacaag
     4561 aacgagctgc tgcgcgcggt ctgcagcgag cagttccgcc agctcgccga gcggaacgaa
     4621 gcagccgccg ccagcaccga cgacccggtc gaggcgctgc ggcgctgcgg gcgcgcctat
     4681 gttgccttcg ggctggagaa ccccgaggcc tacaggatcc tcttgatgtg ccatgaccgc
     4741 gagggacctg aacgcgccca gccggaggcg gtcgccaacg ccgcggcctt cttcgacctg
     4801 gtccagcggg tccagcgcgc gatcgacgcc ggcgccctcg gtcccgtcga cccggtccag
     4861 gccgccctgg gcctgtgggc gaccgcccat gggctcacct cgctgctgat cgcccatccc
     4921 gccttcccct ggccacagga ccgtgcgcag ctcatcgacc gcatcctcga ccaggcgctg
     4981 accggtctgc tcgccggata gcccgcggat agccctgctc agcaggggct cggctgccgt
     5041 acgccgtccg ggaagaccac ctcgtgggcg gcgacgaggc gcctcgagtt cgccgggcag
     5101 gcgaggccgc tggcaacccg gagcagctcg gcgtcccagg cggtcccggc cgaggacttg
     5161 tccggggcga gccccgaggg gttgttcacc gccacggcgt aggagatcgc cccttccgac
     5221 caggccagca ccaccacctc ttggagggtg ccgcagtcgc gccccgccgc cgcgcagagt
     5281 gccgaggccg tgacgatcac cggatggtca ggctgctggc cggcccgggc ccggaagggt
     5341 gtagcgcacg acaaagccat gtgcggccag ctccacgccc ttcacggcga gccgcgcccc
     5401 cgccggcagg taggtcggtt gccggtagcg cggcgtgagc gacggcgccg cgactggctc
     5461 cggcacctgg aacgctccgg tgagcagggg gttcgtcgtg accttcacat cccaccagcg
     5521 ggcgctggcc gagctggagc catccttgcg cacgacggcg gcgccgaccg cggtggcacc
     5581 ctcggcgacg gcggtgaacc cggcggcggt agtgccgtcg ccgaacacct cggcgaagtc
     5641 gcggcgcagg accgagccgc ccagggtggc aggcgcctgc cagcccagct tgccgcgcaa
     5701 cgcgaccatc agatgcgcgc caggcggcag gatcgccggg tccgaggtgg gctggagcca
     5761 gtcgcccagc agcccgcacg gtgcgccatc gacctcctgg tagagcttgc agctcaccgc
     5821 actggcgctg cggggcagct cgatccgggc cgtggtcgcc gtggtcatgg agtcaccccg
     5881 ttttggatcg aggaccatga cgttcgcgtc ggcgccgccc gctgccttcg gcgtcagcac
     5941 ggtccagtgg ccgtcccgca gctcaccctg gaccaccgcg agcccggcgg ccaaggcgac
     6001 cagcagcgcc accgcgccga ccggcgcgac cgcacagcgc tcccgcgcac gcgccagcag
     6061 cccccgacgg cgcggccatc gtggacggcg agcgcccgcg ggcgggcggg tgccggcgtg
     6121 cggacgcgtc caggtcctgc cgtcccgccc cggccactgg ctggcatgct cgcatgccct
     6181 gcactgttcg agcacgcgcc gcagccgctc ctcggtgctc aggcggtgcg gagattcgtc
     6241 gacctgctcg ccgagggcct gcaggccctc gcgaagctcc ccctcggtca gccgcacggc
     6301 cggcctccct cctcctctgt tgctgtcctc tgttgctgtc gctgggtcgt cgcgttcggc
     6361 caccggtcgg aggtgcccgg cccgcacggc ggccggggtc cgccacgggt ggccgtccag
     6421 cgccaaagcc acgcgcagct tggccagcgc ccgggcgctc tggctcttca ccgtcccgac
     6481 cgagcagccg agcagggcgg cggtgtcggc ctcgctcagg tcgtcgaagt agcggagcac
     6541 caccacggtg cgctgccgca ccggcagccg ggcgagcacg cgccgcaggc ggtcacgctc
     6601 gtccacctcg gcgcagccgt ccgccaccgg ccggtcggcg agcgagtcgg ccggctgctc
     6661 cagccgccag cggcgccgcc accaggtcac ctgggtgttg accatcaccc ggcgcacgta
     6721 ggcgtcgacc agctcgcggt cgcggatccg ctcccaggcg agcagcgtct tgaccagcgc
     6781 ggtctgcaac aggtcctcgg ccagcatggc atccccggtg aggagatagg cggtgcgcag
     6841 caacgccggc gagcgcgccc gcacgaaggc gctgaaggcc gcctgctcct cgtcggtcac
     6901 atacgcctcc tcacacgaag gagacgctcc ggcaagccac gaaggttgcc cggcgtgtcc
     6961 cggtgtgggc agtacatgaa ccgcgtcggc gccacggccg cgttcgcggg agaccgttgc
     7021 cctggtccag aacaagtctc ccacccgccc cgcgcccgcg ctgtgccaga cggcacacgg
     7081 gggatgcatc ggcgcggtcc ggtagaaacc ttcggatcgc tccgataaga atttcagtgt
     7141 atttcccgcg ctatgcgcat accgtctagc gttccggcat caacccacaa cgccgcaccc
     7201 agacccagag agcgccgcag agggggcacc catggccaca ccacgcaacc gcaccgccag
     7261 cgaccacaac tccggacacg cgctcaacgc cgccatcagc cagctctaca tccgctgtgc
     7321 cgtcaacggc ctggaccccg accagatcca ggccgagatc cgccgcgccc acccccgaat
     7381 ccacccgctg gaggcccgcc gccgcgctat caactggtcc cgccagcagt tcatggacgc
     7441 catcgcggtc gtcctgtggg agcgggatgg cgtcccctcg ccgctgcgcg atcccgacgt
     7501 gttcgagtgg gaacgcaaag gccgcgtgcc gctgcagtac atcgacgtga tctgccgggt
     7561 gctgcgctgc tcggccgctg acatcggctt cccggagtac tccgccgacc accgcccgcg
     7621 cctccaacca caagacgcgc agaaccagga aattcggaca gatctgcggt acgctgctgg
     7681 cgagagcgcg ctgctcgacg cggttgccca gggagcgagc atcgcctaca tcgggatgct
     7741 ccacagcccc ggcatcgacg ccgagcagta cgtcgtacgg gtcgacggga ggtgcgtact
     7801 cgtggaccgc cggacatttg tgcagctcgc gggagcaatc ccggggttcg ccttcctcgg
     7861 gaccatcgac ttcggcctcc agcccgacgg gcgtgcccgg atcgccggag cgctcaccgg
     7921 agctggctcc gtcgatttga gcctggtcgc cgacctccgc cagagccgga aatcctcggc
     7981 caagctcctt gaggccgaac agctcctcga caaggcagac cccagcgaca caccggcctt
     8041 catctaccac ttcaagccgg cgtgggagct tccagtcatc cgtgggcact gcttccgcta
     8101 cctcggccgc tgggaagagg cagccaacgc ccaaaccgaa tgccttggcg cctacccagc
     8161 agaccgcatc cgcgatcgcg gcgtcgccca cgcccgcaga tccctcgcct ccctcctcga
     8221 cggccacgcg gacgccgcct gcgccgaggc ccagctcgcc ctccccatcg ccctcgaaac
     8281 cgcctcagcc cggatcctga acgacctccg caccctccgc gccaccgccc tgcgccgctg
     8341 gcccgagtac ccctccgtcc gggacctcaa cgacctcctc agccccgtcg cgtaggaggc
     8401 cgcaaggcgg ccactccaca cgaccgctgt cgcatgcggg tttgccgggt gtggcggata
     8461 gccgctagca tcctgcgttg gaagcacccc tttaagggct ggtcc
//
LOCUS       DASWDC010000078         3328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1351396, whole genome shotgun sequence.
ACCESSION   DASWDC010000078 DASWDC010000000
VERSION     DASWDC010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351396"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..706
                     /locus_tag="VF995_02465"
     CDS             146..706
                     /locus_tag="VF995_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342466.1"
                     /translation="MNPGIQTESDTGAPPPAPGPAPRWARWYAGMFLAAFLVCGVVGI
                     EAWPLTGWRLFADARSARETSFQAATVDHAGRETPIRFQDLPIRDHGNVQVLKGFAAL
                     SPAKQAAVCAAWADAVRAQGGDVAALRVYQLDVDLSQREGQRGAPTRRTLRWTCQPPP
                     QGSGSGAGGGSGQGAGQGWRVDAAAG"
     gene            690..1664
                     /locus_tag="VF995_02470"
     CDS             690..1664
                     /locus_tag="VF995_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342467.1"
                     /translation="MRRRVEGLWATFQAWLFAPGDPRRLAALRIGLAGLLTIRLASGP
                     YLDLAHQPAALFRPISFLKLLPHMPPPGVVVPLQVVGVAAALLATVGLWTRVALPVAY
                     LAALPLIAMTSSLGKTVHNDVLLLLCLFPLLPAPAGDAWSLDLRRRPPAERRPAWSWR
                     YGWPPRTAMVVIAGAYSFTGYAKLLHAGPAWVTSGNLRWVLYGSSDAQAHPNVAALFV
                     ADRPWLAHLFAAGTLAVELGFPLVLWRPRLAWLFVPAVVALHTGIWVAMRLDYYPMAA
                     TVLIVFVDWPALVSGRLPSGPFPGGSRSGRRGRPGGSRSPAPGRAALR"
     gene            1898..2908
                     /locus_tag="VF995_02475"
     CDS             1898..2908
                     /locus_tag="VF995_02475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006377877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9342468.1"
                     /translation="MAVAPRELGRSGIRVSAMGFGCWAIGGPFWQGSQPLGWGEVDDD
                     ESVRALRRAFDLGISFFDTADVYGAGHSERVLGRALAEAGARDQVAIATKFGNRFEEA
                     TRQLTGSDASPAYIRQACEASLRRLGTDRIDLYQLHLGDLDPAAAEAAFDTLEALAEQ
                     GLIRAYGWSTDDPERARLLAGRPHFAAVQHDLNVLLDAPEVLAACDQLGVASVNRGPL
                     AMGLLSGKFQADSRLPADDVRGKEPIWMRYFQDGRPSPAWLARLDAVREVLTSGGRTL
                     AQGALAWIWARSERTIPIPGVRTVPQVEENAGALAFGPLEPGQLTEIDALLGRTTAGA
                     QP"
     gene            complement(2956..>3328)
                     /locus_tag="VF995_02480"
     CDS             complement(2956..>3328)
                     /locus_tag="VF995_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9342469.1"
                     /translation="TSAFSQHAAIAALEGPWDPVEAMVAAFRARRDVIVTGLNRIPGV
                     SCLQPQGAFYAFPRVADLGLPAAELAGALLERAGVACLPGTAFGAYGEGYLRFSYANS
                     IPNIEAALAAMEDLTAEVAQA"
BASE COUNT          416 a         1243 c         1195 g          474 t
ORIGIN      
        1 atggaaagcc tcggcctgga ggggcgcggc ggcgcggtcc gcgccgggtt cctgcactac
       61 aacacccccg ccgaggtcga ccgcttcctg gaggcgctgc accacatcgc cacctgaccc
      121 ctggccggtg cggtagcgtc cacccatgaa cccgggcatc cagaccgaga gcgacaccgg
      181 ggcgccgccg ccggcgccgg gcccggcgcc gcgctgggcg cgctggtatg ccgggatgtt
      241 cctggccgcg ttcctggtct gcggagtggt cgggatcgag gcctggccgc tcaccggctg
      301 gcggctgttc gcggacgcgc gcagtgcccg cgagaccagc ttccaggccg ccaccgtcga
      361 ccacgccggc cgcgagaccc cgatccgctt ccaggatctg ccgatccgcg accacggcaa
      421 cgttcaggtc ctcaagggtt tcgccgccct gtcgcccgcc aagcaggcag ccgtgtgcgc
      481 cgcgtgggcg gacgccgtcc gcgcccaggg cggcgacgtc gcggcgctgc gcgtctatca
      541 gctcgacgtg gacctgtccc aacgggaggg gcagcggggc gcaccgaccc ggcgcacgct
      601 gcgctggact tgccagccgc cgccgcaggg ctccggctcc ggagccggcg ggggttccgg
      661 ccagggcgca ggccagggat ggagggtcga tgcggcggcg ggttgaggga ctgtgggcga
      721 ccttccaggc ctggctgttc gctccgggcg acccgcggcg gctcgcggcg ctgcggatcg
      781 ggctcgccgg gctgctgacg atccggctcg ccagcgggcc ctacctcgac ctcgcccacc
      841 agccggcggc gctgttccgg ccgatctcct tcctgaaact gctgccgcac atgccgccgc
      901 cgggcgtggt cgtcccgctc caggtcgtcg gggtggccgc cgcgctgctc gccacggtgg
      961 gactgtggac ccgggtggcg ctgccggtcg cctacctcgc cgcgctgccg ttgatcgcca
     1021 tgacctccag ccttggcaag acggttcaca acgacgtgct gctgctgctg tgcctgttcc
     1081 cgctgctccc cgcaccggcg ggggacgcct ggtcgctgga ccttcggcgg cgccctccag
     1141 ccgaacgacg ccccgcgtgg tcgtggcgct acggctggcc gccgcggacc gccatggtgg
     1201 tgatcgctgg cgcgtactcc ttcaccggct acgccaagct gctccacgcc gggccagcct
     1261 gggtgaccag cgggaacctc cgctgggtcc tctacggctc cagtgacgcg caggcccatc
     1321 ccaacgtcgc cgcgctgttc gtcgccgacc ggccctggct cgcccacctg ttcgccgccg
     1381 gcaccctcgc ggtcgagctg ggcttcccgc tggtgctgtg gcgcccgcgg ctggcctggc
     1441 tgttcgtccc cgccgtggtc gccctccaca ccggcatctg ggtggcgatg cgcctcgact
     1501 actacccgat ggccgccacc gtcctgatcg tgttcgtcga ctggccggcg ctcgtcagcg
     1561 gtcgccttcc atccggtcca ttccctggag gatcgcgctc cgggcggcgc ggacgccctg
     1621 gcggtagccg atcgccagca cctggtcgag ctgcgcttcg ctgatggtgc cgtaggcgcg
     1681 cacctgggcc cggatcgttt cgaggtactg ggcgtcgtag ccggcccgga cggtggacgg
     1741 gaggtcgtac gggtcatcgc tcatccgcga attctcgcat cctcgcagca tcggacgaaa
     1801 acggagagtg cttgccttgc ctgtcgtgct gcctgcttcg cctctaccct gagccgcgac
     1861 gaaaccggac aggaacgaag aatcaggagg gaagcgcatg gcggtggcgc cacgcgagct
     1921 gggtcgcagc gggatccggg tgagcgcgat gggctttggg tgctgggcga tcggcgggcc
     1981 gttctggcag ggaagccagc cgctcggctg gggcgaggtg gacgacgacg agtcggtgcg
     2041 ggcgctgcgg cgggcgttcg acctggggat cagcttcttc gacaccgccg acgtctacgg
     2101 cgccgggcac agcgagcggg tcctcgggcg agccctggcg gaggcgggtg cgcgcgacca
     2161 ggtggcgatc gccaccaagt tcggcaaccg cttcgaggag gccacccgcc agctcaccgg
     2221 cagcgacgcc agccccgcct acatccgcca ggcgtgcgag gcgtcactgc ggcggctcgg
     2281 gaccgaccgg atcgacctgt accagctgca cctcggcgat cttgacccgg ccgccgccga
     2341 agccgccttc gacaccctgg aggcgctggc cgagcagggc ctgatccgcg cctacggctg
     2401 gagcaccgac gaccccgaac gcgcccgcct gctcgcggga cggccgcatt tcgccgccgt
     2461 ccagcacgac ctgaacgtgc tgctggacgc gcccgaagtg ctggccgcct gcgaccagct
     2521 cggcgtggcc agcgtcaacc gcggcccgct ggcgatgggg ctgctcagcg gcaagttcca
     2581 ggccgactcg cgcctgcccg ccgacgacgt gcgcggcaag gagcccatct ggatgcgcta
     2641 cttccaggac ggccgcccaa gccccgcgtg gctcgcccgc ctcgatgccg tccgcgaggt
     2701 cctcaccagc ggcggccgca ccctggccca aggcgccctc gcctggatct gggcccgcag
     2761 cgagcgcacc atccccatcc caggcgtccg caccgtcccc caagtcgagg agaacgccgg
     2821 cgcgctcgcc ttcgggccgc ttgaacctgg ccagctcacc gagatcgacg ccctgctcgg
     2881 ccgcaccacc gctggcgcgc agccataggc tctggaaggc ccgcatcccg cccgggcgag
     2941 gtcccggttc ccctgtcagg cttgggcaac ttccgcggtc aggtcttcca tcgccgccag
     3001 ggcggcctcg atgttgggga tcgagttggc gtaggagaag cgcaggtagc cctcgccgta
     3061 ggcgccgaag gcggtgcccg gcaggcaggc gaccccggcg cgttccagca gcgccccggc
     3121 cagctcggcg gcgggcaggc cgaggtcggc gacccgtggg aaggcgtaga aggcgccctg
     3181 cggctgcagg cacgagacgc cggggattcg gttcaggccc gtgacgatca cgtcgcggcg
     3241 ggcgcggaag gcggcgacca tcgcctcgac cggatcccag gggccctcca gcgcggcgat
     3301 cgcggcgtgc tggctgaacg ccgaggtg
//
LOCUS       DASWDC010000079         4656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1268639, whole genome shotgun sequence.
ACCESSION   DASWDC010000079 DASWDC010000000
VERSION     DASWDC010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268639"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VF995_02485"
     CDS             <1..356
                     /locus_tag="VF995_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342470.1"
                     /translation="RRVYRLFPARGPSQRLNAGRSRFCAMRKTCPARFGVLASSLRLS
                     GELAREVLRVLREEDPGLPEDSTVVALVVEPRHANQQPPPPAAGLEGLIGVLGDVEVP
                     TDASERVDEYLYGRR"
     gene            510..773
                     /locus_tag="VF995_02490"
     CDS             510..773
                     /locus_tag="VF995_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9342471.1"
                     /translation="MGRTYRAFDEVLRRIENGSLTVLDLNSDDYARVRHLCAQYGDWP
                     LGFVDAAVVALAERERQTRIATLDRRHFGAIKPAHVPAFELLP"
     gene            complement(808..948)
                     /locus_tag="VF995_02495"
     CDS             complement(808..948)
                     /locus_tag="VF995_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342472.1"
                     /translation="MAGALSLGMASAQAASGTKSAPTQAPTAPATGGCPHHSSTLSTP
                     SV"
     gene            complement(1142..1867)
                     /locus_tag="VF995_02500"
     CDS             complement(1142..1867)
                     /locus_tag="VF995_02500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9342473.1"
                     /translation="MTVPAAGLATPGPAAERTARGVRRMFDRVAPRYDLANTVLSLGQ
                     DRHWRSAAAGATGLGDGEAAVDVACGTGKLAADLARLAPGATVIGLDFSVEMLRRAGG
                     SVPVAAGDALALPLADASVDVVTIAFGLRNLPDPAQGLREFRRVLRPGGRLVVCEFSH
                     PVLPGFREVYERYLNRLLPLAARRLSPDPEAYEYLARSIAAWPSQPDLARWLQRARFS
                     AVRWRSLTGGIVALHHGTVPTVP"
     gene            complement(1889..2731)
                     /locus_tag="VF995_02505"
     CDS             complement(1889..2731)
                     /locus_tag="VF995_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX9342474.1"
                     /translation="MPRPRPRRAVSLAALTLVALLVFVAAMMATALSASAAPALPNSI
                     AAIGDSISQAFDSCCLWGDHPSHSWSTGYDSGDGITSHYEHVLAANGGISGHYHDDAV
                     TGAKMAAGPGQAASAVSQGAQYVTVLLGANDVCTSSSSNMTSTTDFTNQFTSTMNTLQ
                     GLPSGAHVFVSSIPNVYQLWATLHNNILAEAVWKVASICQSMLNTSNSEATRQAVLAH
                     ERDLNNVLQSVCARYSNCRFDNFAVFNTSFSSSQVSGLDYFHPNQSGQALLANVTWHA
                     SYWG"
     gene            complement(2971..3432)
                     /locus_tag="VF995_02510"
     CDS             complement(2971..3432)
                     /locus_tag="VF995_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9342475.1"
                     /translation="MASKDPHLDAIDLSILRVLQVNARATYTEIGKAVGLSAPSAHDR
                     VHRLEDRGVIRGYHAAIDPELLGLDVLALVGVVPSDTANLAAMEGAFAAAGQIEAAYT
                     VTGEESHLLAVRAHSLMELSEVLQRIRDVDGVAHTRTAVVLSTPFLRGPAL"
     gene            complement(3479..4471)
                     /gene="ccsB"
                     /locus_tag="VF995_02515"
     CDS             complement(3479..4471)
                     /gene="ccsB"
                     /locus_tag="VF995_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEX9342476.1"
                     /translation="MSPASLANLSETLVWFAVIAYVVAAVLYGLEFAYRIHWTGRAGL
                     AVTIAGLGANVAAAVTRGLAAHRVPWGNMYEYSIMVGVILVGGFLVWARRRPEVRPLG
                     VFFLFPAVLAIGIAGVALRVPAGPLVPALNSGWIKIHVAAAITGSSVLSLSAIFSALF
                     LVKDRVERRRETRLPPLVAGGARTADQVELQEALAGYEAEAEVEAEPTADPQAEGLAP
                     RSLWDRLPAARTLDELAYRTAAFGFPIWTFAIIAGAIWAQSAWGRYWGWDPKETWSFI
                     VWVVFAAYLHARATVGWRGRRAAVISLVGMGCLLFNFYVVNTWIVGLHSYAGVK"
BASE COUNT          712 a         1540 c         1712 g          692 t
ORIGIN      
        1 cgcgcagagt ctacaggctt ttccccgctc gtgggccgag tcagcgactg aacgcgggca
       61 ggtcccgatt ctgcgccatg aggaagacct gcccagcgcg gttcggcgta ttggcttcca
      121 gcttgcgcct ctccggagag ctcgcccgtg aggtcctccg tgtcctgcgt gaggaggacc
      181 cagggctgcc ggaagactcg acggtcgtgg cgcttgttgt cgaaccacga cacgcaaacc
      241 agcaacctcc tcctcctgcc gccgggctcg aagggttgat cggcgtcctc ggcgatgttg
      301 aggtgccgac ggacgcgtcc gaacgtgtcg acgagtacct ctacggccgc cgttgatcgt
      361 cgacaccgga gccctctacg cggccatgtc tcctcgtgac cgtcaccatg tgccttgcgc
      421 ggcactgctg cggacgggag agcaattcct gctgcctgcg ccagtcctgg tcgagctgga
      481 ctatttcctt atggagctga ccggaacgta tcgggcgaac gtatcgggcg ttcgacgagg
      541 tgctccgccg gattgaaaac ggctcgctca ccgtcttgga cctgaacagt gacgactacg
      601 ctcgggtccg gcacctgtgt gcacagtacg gcgattggcc gctcggcttc gtcgacgcgg
      661 ccgtggtggc gctcgcggag cgagaacggc agacgcgaat cgccaccctg gatcgccgcc
      721 acttcggggc gatcaagccg gcgcacgttc cagccttcga attgctgccg tagccgcggc
      781 ttccgcccga cgcctcaggt acaaccctca gacggagggc gtgctgaggg tggaggagtg
      841 gtgcgggcag cctcctgttg ccggggcggt gggagcctgg gtcggggcgg acttggtccc
      901 gctggcggcc tgggcgctgg ccatgccgag gctcagggct ccggccacgg cgaggacgag
      961 gagaatcctt cggacggtcg gtcgtgacaa caccggtacc tcccgtttgt agcgatacct
     1021 cagtgtaaca gccaccgatg gggtcgagac tccggcctga gcatgctgtt ccggcctacc
     1081 gggatgagat cgagcagtgg atcagcgaca gtgcgcatgg cctccagggc ggcatccatg
     1141 ctcagggcac ggtcgggacg gtgccgtggt ggagggcgac gattcccccg gtgaggctgc
     1201 gccagcggac ggcgctgaac ctggctcgct ggagccaacg ggcgaggtcg ggctggctgg
     1261 gccaggcggc gatcgagcgg gcgaggtact cgtaggcttc ggggtcgggg ctgaggcgcc
     1321 gcgcggcgag ggggagcagg cggttcaggt agcgctcgta gacctcgcga aagcctggca
     1381 gcaccgggtg ggagaactcg cagacgacca ggcgcccccc ggggcgcagg acgcggcgga
     1441 actcccgcag gccctgggcc ggatcgggga ggttgcgcag cccgaaggcg atcgtgacca
     1501 cgtcaaccga ggcgtcggcc agcgggaggg ccagcgcgtc cccggcggcg accggaacgc
     1561 tgccgccggc ccgccgcagc atctccaccg agaagtcgag gccgatcacg gtggcgcccg
     1621 gggcgaggcg ggcgaggtca gcggcgagct tcccggtgcc gcaggcgacg tcaaccgcgg
     1681 cctcgccgtc acccagcccg gtggcgcccg ccgccgcact gcgccagtgg cggtcttgcc
     1741 ccagcgaaag cacggtgttg gccaggtcgt agcggggtgc gacgcggtcg aacatgcgcc
     1801 gcacccctct ggcggtccgc tccgccgccg ggccgggggt cgccaggccg gcggcgggca
     1861 ctgtcatgtg gcgctcacgc gcctgagctt acccccagta ggaggcgtgc caggtgacgt
     1921 tggcgaggag cgcctggccg ctctggttcg ggtggaagta gtccaggccg gagacctggc
     1981 tggagctgaa cgaggtgttg aacaccgcga agttgtcgaa gcggcagttc gagtacctgg
     2041 cgcagacgct ttgcaggacg ttgttcaggt cccgctcgtg ggcgaggacc gcctgccggg
     2101 tcgcctcgct gttggaggtg ttgagcatcg actggcagat gctggcgacc ttccagaccg
     2161 cctcggcgag gatgttgttg tggagcgtcg cccacagctg gtagacgttc gggatgctcg
     2221 acacgaagac gtgcgccccg gacggcagac cctgcaaggt gttcatggtg ctggtgaact
     2281 gattggtgaa gtcggtcgtc gaggtcatgt tcgacgacga cgaggtgcag acgtcgttgg
     2341 cgcccagcag gacggtcacg tactgcgccc cctgggacac tgcggacgcc gcctggcccg
     2401 gcccggcggc catcttcgcg ccggtgacgg cgtcgtcgtg atagtggccc gagatgccgc
     2461 cgttggcagc gaggacgtgc tcgtagtggc tggtgatgcc gtcgccgctg tcgtagccgg
     2521 ttgaccagga atggctgggg tggtcacccc agagacagca ggagtcgaac gcctggctga
     2581 tcgagtcgcc gatggcggcg atcgagttcg gcagggctgg ggcggcgctg gcactgagcg
     2641 cggtcgccat catggccgcg acaaagacca gcagggcgac gagggtgagg gcggcgaggg
     2701 acaccgcgcg gcggggacgt ggccggggca tggccaactc cttccggggg ggcgagcatg
     2761 ccaacctcgc gacgcggcta cgcgcgcggg gggaaggcat gcgacctatg ggaggagcct
     2821 ggggggctcg ctacccttga ttgggcggct tcggactcgt ccgtgtcaag ggtctatgcg
     2881 aattccaccc acttcgcccg cagatgcccg gggttgcgaa ggtgttgcag gggattcgca
     2941 ggggatttgc agcggttgtt gcggcgccgc tcacagcgcc gggccgcgca ggaacggggt
     3001 ggacaggacc accgcggtgc gggtgtgggc caccccgtcg acgtcgcgga tgcgctgcag
     3061 cacctcggac agctccatga gggagtgggc gcggaccgcc agcaggtggc tctcctcgcc
     3121 cgtcacggtg taggccgcct cgatctggcc ggccgcggcg aaggcgccct ccatcgccgc
     3181 caggttggcc gtgtcgctcg ggaccacccc gaccagcgcc agcacgtcca gaccgagcag
     3241 ctcggggtcg atggcggcgt ggtagccgcg aatcacgcca cggtcctcca gccggtgcac
     3301 ccggtcgtgg gccgagggcg ccgacaggcc gaccgccttg ccgatctcgg tgtaggtggc
     3361 gcgggcgttg acctgcagca cccggaggat gctcaggtcg atcgcgtcga gatgcggatc
     3421 cttgctggcc atctggcctg gttctgcccc atccgagcgc tccgcaatcc gcgggcgcct
     3481 atttcactcc cgcgtagctg tggaggccga cgatccaggt gttcaccacg tagaagttga
     3541 acagcaggca gcccatgccg accagggaga tgacggcggc gcggcggccc cgccagccga
     3601 cggtggcgcg ggcgtggagg taggcggcga acaccaccca gacaatgaac gaccaggtct
     3661 ccttggggtc ccagccccag tagcggcccc aggcggactg cgcccagatc gccccggcga
     3721 tgatcgcgaa ggtccagatc gggaagccga aggcggccgt ccggtaggcc agctcgtcga
     3781 gcgtccgtgc cgccgggagg cggtcccaga ggctccgcgg ggccaacccc tcggcctgcg
     3841 ggtccgctgt gggctcggcc tcaacctcag cttcggcctc gtagccggcg agcgcctcct
     3901 gcagctccac ctggtcggcg gtccgcgctc cacccgcgac caggggcggc agcctggtct
     3961 cgcggcggcg ctcgacccgg tccttgacca ggaacagcgc gctgaagatc gccgagaggc
     4021 tgagcaccga cgaaccggtg atggcggcgg cgacgtggat cttgatccag ccggagttga
     4081 gcgccggcac cagcggcccg gcgggcaccc gcagcgccac cccggcgatg ccgatcgcca
     4141 gcaccgccgg gaacaggaag aagaccccca gcggccggac ctcgggccgg cgcctggccc
     4201 acaccaggaa gccgccgacg aggatgaccc cgaccatgat cgagtactcg tacatgttgc
     4261 cccacggcac ccggtgggcg gccaggccgc gggtgaccgc cgcggccacg ttggcgccca
     4321 ggcccgcgat ggtgacggcc agcccggcgc ggccggtcca gtggatgcgg taggcgaatt
     4381 cgaggccgta cagcaccgcg gccaccacgt aagcgatgac ggcgaaccac accagcgtct
     4441 cggagaggtt cgccaggctt gccggggaca tgaactcctc ctacgtacct cttgacctct
     4501 tggccttcac cgccggaccg aggatgaccc gctcagggta gacccagcct gctccccggg
     4561 cgactcgaac ccgggcccga gcaattcgcg gcggacctcg tcggcgatct ccttgaactc
     4621 ctccacgaag gcgggtttcc cctggagggc gagccc
//
LOCUS       DASWDC010000080         5660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_518034, whole genome shotgun sequence.
ACCESSION   DASWDC010000080 DASWDC010000000
VERSION     DASWDC010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518034"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /gene="speB"
                     /locus_tag="VF995_02520"
     CDS             <1..950
                     /gene="speB"
                     /locus_tag="VF995_02520"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX9342477.1"
                     /translation="DPLSVPRFAGPSTFARLPRRDEVERCDVAVVGVPFDSGVTYRPG
                     ARFGPAHVRAGSRLLRPYHPGLDVEPFAVQQVADAGDIACNPFDIQEAIGQIQRAADG
                     LLADSGCLVAIGGDHTIALPLLRAAHHLWGPVALLHFDAHLDTWDTYFGAPYTHGTPF
                     RRASEEGLLRRDRSVHVGTRGPLYAPTDLTDDADLGFQVIDVMTVGRDGPDAIAERIR
                     GQVGDAPLYVSVDIDVLDPAHAPGTGTPEAGGLSSRELLAILRGLAARDTAEHPAARI
                     VGVDVVEIAPAYDHAEITAIAAAHVIYELLSVLAVGRTG"
     gene            complement(984..1604)
                     /locus_tag="VF995_02525"
     CDS             complement(984..1604)
                     /locus_tag="VF995_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9342478.1"
                     /translation="MSSEVATQPEPGTAGPGLRERKKARTRAAIRQHAMRLFHEQGYD
                     ATTVEQIAAAAEVSPSTFFRYFPAKEDVVLADDLDPPLIAAFQAQPSELPPIAALRGA
                     MRTVIDALPPEEWAQERARIALIRSVPELRARMMEGFATQLQLAAEIVAERVGRRPDD
                     FEIRVLTGALLGIVLATWFSWSEESGTDYYTVLDAALAHLEAGLPL"
     gene            complement(1594..3153)
                     /locus_tag="VF995_02530"
     CDS             complement(1594..3153)
                     /locus_tag="VF995_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9342479.1"
                     /translation="MISLGSRRWWALGALALALLAVGLDLTVLNLALPTLATDLHASN
                     AQLQWFIDAYTLVLAAMLLPAGLLGDRYGRKKLLVGALALFGVASLGCAYATSAGGLI
                     AARAVLGLGGAFLIPLSMAVLPVLFTAEERPKAIAVWVAANAVSWPIGPILGGWLLDN
                     YWWGSVFLINVPVTVVGLIAVAVLLPESRSIHRPRLDITGVLISSAGLVGLTYGAIEA
                     GQHGWGSPAAAASLIAGVLLLVVFILVQRRISQRPEGQPLIDLSLFRSASFTWGTTLA
                     TLVSFALFGVLFAVPQYFQAVGGTTAMGAGLRLLPIIGGLLVGSQVAERLARRAGAKV
                     TVATGFVLLAAGLATGAASGVHSGYGFAATWVSIVGIGMGFTLPTSMDAALSALSPER
                     SGTGSGVIQALRQVGGTIGVAVLGTVLSSAYHARLDLTGLPTQAASAVRDSVSAGVAV
                     AHQLGSTPLLDSVRAAFAHGMDVMLLACGGIALLGIVLTLAFLPRRALVPAADAAATT
                     SQSEPESEIVV"
     gene            complement(3225..4532)
                     /locus_tag="VF995_02535"
     CDS             complement(3225..4532)
                     /locus_tag="VF995_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEX9342480.1"
                     /translation="MALATVVFFPEGAFGPTNNCVGIGDVLRRRGHRVVFIVEESFAG
                     TLAAKGFEERLMRLGPPSEVEEAPGQFWKDFIRDTAPVFRRPTIEQLEGFIAPTFQAL
                     VDGARYVDDRLREIIGEIQPDVLVEDNVVAFPALPASGRPWVRITSCNPLELKDPDLA
                     PVFSGYPEADRSGWAAFRAEYRRTHSALYDDFSQFCQDRGAPPLPELEFIHESPWLNL
                     YLYPREADYRRRRPLAATWHNLEACVRATDQPWELPASLAGGPEAGPLLYLSLGSLGA
                     ADVELMQHLIDVLGRTRHRVIVSMGPQHELLRLADNMAGAEFLPQVSILPQVDLVITH
                     GGNNTTTECLWFGKPMVLLPLFWDQYDNAQRMQEAGFGARLPTYAFEEGELVAAVERL
                     PADQALTARLQTTSQRLRASPGTVRAADLIERLAKEQAAVTDG"
     gene            4718..5608
                     /locus_tag="VF995_02540"
     CDS             4718..5608
                     /locus_tag="VF995_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX9342481.1"
                     /translation="MHPPRFDYVAPRELGEALDHLAEHGDDAKVLAGGQSLIPLLKLR
                     FAGPALLVDVNRIEGLDTLAEHDGWLRVGALVRHRDAEHAALLRERYPTMAAAAPQVS
                     DPLVRNLGTVCGSLCHADPSGDWGAVMLALSAQLLARSRDGERTIPAREFFAGPFETT
                     LRPDELLVEARVPAPATRRGGGAYLKLERKVGDFATAAVAVHLSMANGHIGAAGIGLT
                     AVGPTNLAATEADAVLVGAEPSDELFREAAELAAQAAQPSDDQRGPAAYKRNVVRVFT
                     ERGLRAAAAMARTQDTGGGA"
BASE COUNT          846 a         1973 c         2095 g          746 t
ORIGIN      
        1 tcgacccgct ctcggttccg cgcttcgccg gcccatccac gttcgcccgg cttccccgcc
       61 gcgacgaggt ggagcggtgc gacgtcgccg tggtcggggt ccccttcgac agcggcgtca
      121 cctaccgccc cggcgcccgc ttcggccccg cccacgtccg tgccgggtcc cggctgctgc
      181 gcccctacca ccccggcctc gacgtcgagc cgttcgccgt ccagcaggtc gccgacgccg
      241 gcgacatcgc ctgcaacccc ttcgacatcc aggaggccat cggccagatc cagcgcgccg
      301 ccgacggcct gctcgccgac agcggctgcc tggtcgccat cggcggcgac cacaccatcg
      361 cgctgccgct gctgcgcgcc gcccaccacc tctgggggcc ggtcgccctg ctccacttcg
      421 acgcccacct cgacacctgg gacacctact tcggcgcccc ctacacccac ggcaccccct
      481 tccgccgcgc cagcgaggag ggcctgctgc gcagggaccg ctccgtccac gtcggcaccc
      541 gcggccccct ctacgccccc accgacctca ccgacgacgc cgacctcggc ttccaggtga
      601 tcgacgtgat gaccgtcggc cgcgacggcc ccgacgccat cgccgagcgc atccgcggcc
      661 aggtcggcga cgcacccctg tacgtctcgg tcgacatcga cgtcctcgac ccggcccacg
      721 cccccggcac cggcaccccc gaagccggcg gcctctccag ccgggagctg ctcgcgatcc
      781 tgcgcggcct cgccgcccgc gacaccgccg aacaccccgc cgcccgcatc gtcggggtcg
      841 acgtagtgga aatcgcaccg gcctacgacc atgcggagat caccgcgatc gctgcggcgc
      901 atgtgatcta cgagttgctg tcggtgctgg cggtcgggcg gacgggctga cgctgccgcg
      961 tgcggccggg ccggccggcc aggctagagc ggcaggccag cttccaggtg cgcgagggcg
     1021 gcgtcgagca ccgtgtagta gtcggttccg gactcctcgc tccagctgaa ccaggtggcg
     1081 agcacgatcc caaggagcgc gccggtgagg acgcgtatct cgaagtcgtc gggacggcgc
     1141 ccgacccgct cggcgacgat ctcggcggcc agttggagct gcgtcgcgaa cccttccatc
     1201 atccgggcgc gcagctcggg gacggagcgg atcagggcga tgcgtgcccg ttcctgggcc
     1261 cactcctcgg gtgggagcgc gtcgatgacg gtgcgcatcg cgccgcgcag ggcggcgatc
     1321 ggcggcagtt cgctgggctg ggcctggaag gccgcgatca ggggcgggtc gaggtcgtcg
     1381 gcgagaacca cgtcttcttt ggcggggaaa tagcggaaga aggtgctggg cgagacctcc
     1441 gccgccgcgg cgatctgctc gacggtggtg gcgtcgtagc cctgctcgtg gaacagccgc
     1501 atggcgtgct ggcggatcgc ggctctggtc ctggccttct tgcgttcgcg cagcccgggc
     1561 ccggcggtac cgggctcggg ctgcgtggct acctcagacg acaatttcgc tctccggttc
     1621 cgattgagac gtcgtcgccg ccgcgtcggc tgcgggtacg agggcccgtc ggggcaagaa
     1681 ggccagtgtc agcacgatac cgagcagggc gatgccgccg cacgccagca gcataacgtc
     1741 catgccatgg gcgaaggcgg cgcgcacgga gtccagcagc ggcgtcgagc cgagctggtg
     1801 ggcgacggcg acgccggcgg agacgctgtc ccggacggcg ctggcggcct gggtgggcag
     1861 gccggtgagg tcgaggcggg cgtggtaggc ggagctgagc acggtgccga ggacggcgac
     1921 gccgatggtg ccgccgacct ggcggagcgc ctggatcacg ccggagccgg tgccgctgcg
     1981 ctcgggcgac agggcgctga gggcggcgtc catcgaggtc gggagggtga accccatgcc
     2041 gatgccgacg atcgagaccc aggtggcggc gaagccgtag ccgctgtgga cgccgctggc
     2101 ggcgccggtg gcgagcccgg cggcgagcag cacgaacccg gtcgcgacgg tgaccttggc
     2161 gccggcgcgg cgggcgagcc gctcggcgac ctgcgagccg acgagcaggc cgccgatgat
     2221 cgggaggagg cgcaggccgg cgcccatggc ggtggtgccg ccgacggcct ggaagtactg
     2281 cgggacggcg aacagcaccc cgaacagcgc gaacgacacc agggtcgcca gggtggttcc
     2341 ccaggtgaag ctggcggagc ggaacagcga gaggtcgatc agcggctggc cctctggccg
     2401 ttggctgatg cggcgctgga cgaggatgaa caccacgagc agcaggacgc cggcgatcag
     2461 ggaagcggct gcggcggggc tgccccagcc gtgctggccc gcttcgatgg cgccgtaggt
     2521 gaggccgacc aggccggcgc tggagatgag gacgccggtg atgtccaggc gggggcggtg
     2581 gatgctgcgc gactctggca gcaggacggc cacggcgatg agtccgacga cggtgaccgg
     2641 cacgttgatg aggaagaccg aaccccacca gtagttgtcg agcagccagc cgccgaggat
     2701 cgggccgatc ggccagctga cggcgttggc ggcgacccag acagcgattg ccttgggccg
     2761 ctcctcggcg gtgaacagca ccgggaggac cgccatcgac agcgggatca ggaacgcgcc
     2821 gccgagcccg agcacggcgc gggcggcgat cagtcccccg gccgaggtgg cgtaggcgca
     2881 gccgagggac gccacgccga agagggcgag tgcgccgacg aggagcttct tgcggccgta
     2941 gcggtcgccg agcagcccgg cgggcagcag catcgcggcc aggacgaggg tgtaggcgtc
     3001 gatgaaccat tgcagttggg cgttgctggc gtgcaggtcg gtcgccaggg tcggcagtgc
     3061 caggttgagg acggtcaggt ccaggccgac cgccagcagc gccagggcga gcgcgccgag
     3121 cgcccaccac ctgcgggatc cgagagaaat catgagagct acaacctttc aggagggacc
     3181 ctgaaatgat agtaactctc ataaggtagc gactgtcaag cttttcagcc gtcggtgacc
     3241 gccgcctgct ccttggcgag ccgttcgatc aggtcggcgg cccgcacggt gccgggactg
     3301 gcccgcaggc gttgcgaggt ggtctggagg cgcgctgtca gggcctggtc ggcgggcagg
     3361 cgctcgaccg cggccaccag ctcgccctcc tcgaaggcat aggtgggcag gcgggcaccg
     3421 aagccggcct cctgcatccg ctgggcattg tcgtactggt cccagaacag cggcagcagc
     3481 accatcggtt tgccgaacca gaggcactcg gtggtggtgt tgttgccgcc gtgggtgatc
     3541 accaggtcga cctggggcag gatcgacacc tgcggcagga actcggcgcc ggccatgttg
     3601 tcagccaggc gcagcagctc gtgctggggg cccattgaca cgatcacccg gtggcgggtg
     3661 cggccgagca cgtcgatgag gtgctgcatc agctcgacgt cggcggcgcc gagcgagccg
     3721 aggctcaggt agagcagcgg cccggcctcg gggcccccgg ccaggctggc gggcagctcc
     3781 cagggctggt cggtggcgcg cacgcacgcc tccaggttgt gccaggtggc cgcgagcggg
     3841 cgccggcgcc ggtagtcggc ctcgcgcggg tacaggtaca ggttgagcca gggcgactcg
     3901 tggatgaact cgagctccgg cagcggcggc gcgccgcgat cctggcagaa ctggctgaag
     3961 tcgtcgtaca gcgcactatg ggtgcggcgg tactcggcgc ggaacgccgc ccagccgctg
     4021 cggtcggcct cggggtagcc ggagaacacc ggggccaggt ccgggtcctt cagctccaga
     4081 gggttgcagg aggtgatccg gacccagggg cgcccgctgg ccggcagggc agggaacgcg
     4141 accacgttgt cctcgaccag cacgtccggc tggatttccc cgatgatctc gcgcagacgg
     4201 tcgtccacgt agcgggcgcc gtcgaccaac gcctggaagg tgggggcgat gaagccttcc
     4261 agctgctcga tcgtggggcg gcggaagaca ggcgcggtgt cgcggatgaa gtccttccag
     4321 aactgcccgg gcgcctcctc gacctcggac ggggggccga gccgcatcag gcgctcctcg
     4381 aagcccttgg ctgccaaggt gcccgcgaac gactcctcca cgatgaagac gacccggtgc
     4441 ccgcggcggc gcagcacgtc gccgatgccg acgcagttgt tggttgggcc gaacgcacct
     4501 tcggggaaga acacgaccgt cgccaaggcc acctcctgct cgcgaacctc accgggaacc
     4561 agcgccagat caagcaggtt aacgcaagca ggcaaggcaa tcggacattg acgcattgac
     4621 aggctctctc gcccgacgga gcatcgtggg cgctaggtgc attccttcct gatccaccga
     4681 ttctgtccag atccgccgcc tgtcaggagg aggccgcatg caccctcccc gctttgacta
     4741 tgtcgcgccc cgcgaactcg gcgaggcgct cgaccacctc gccgaacatg gcgacgacgc
     4801 caaggtgctg gccgggggcc agagcctgat cccgctgctg aagctgcggt tcgcggggcc
     4861 cgcgctgctg gtcgacgtga accggatcga aggcctggac acgctcgccg agcacgatgg
     4921 atggctgcgg gtcggggcgc tggtccgcca ccgcgacgcc gaacacgccg cgctgctgcg
     4981 ggagcgctat cccaccatgg cggcggccgc gccgcaggtc tccgacccac tggtgcgcaa
     5041 cctcggcacc gtctgcggat cgctctgcca cgcggacccg tccggcgact ggggtgcggt
     5101 gatgctcgcg ctcagcgcgc agttgctggc ccgctcccgc gacggcgagc ggacgattcc
     5161 cgcgcgggag ttcttcgccg ggccgttcga gaccacgctg cgacccgacg agctgctggt
     5221 cgaggcccgc gtcccggcac cggcgacgcg ccgcggcggc ggggcctacc tgaagctgga
     5281 gcgcaaggtc ggcgacttcg ccaccgccgc ggtcgcggtg cacctgtcga tggccaacgg
     5341 ccacatcggc gcggcgggca tcgggctgac cgcggtcgga ccgaccaacc tggccgccac
     5401 cgaggccgat gcggtccttg tcggtgccga gccgagcgac gagctgttcc gggaggcggc
     5461 ggagcttgcc gcgcaggccg cccagccaag cgacgaccag cgcggccccg cggcctacaa
     5521 gcgcaacgtc gtgcgcgtgt tcaccgagcg tggcctacgc gcggcggccg cgatggcccg
     5581 gacccaggac acaggcggag gtgcgtgagg catgaaggtc accgtcacgg tgaacggcag
     5641 cgagcacagc gccgaggtcg
//
LOCUS       DASWDC010000081        19250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1434422, whole genome shotgun sequence.
ACCESSION   DASWDC010000081 DASWDC010000000
VERSION     DASWDC010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19250
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434422"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VF995_02545"
     CDS             complement(<1..1314)
                     /locus_tag="VF995_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9342482.1"
                     /translation="MPEALYPRRWWTLGVLCLSLLVIGLDNTILNVALPTLVRDLHAS
                     GSQLQWMVDSYALVFAGMLLTAGALGDRFGRRRVLAAGMLLFGTASGLSAFAGSAGTL
                     IAWRAVMGLGAALIMPSTLSIVTNVFPASERGRAIGIWAGVSGLGIAIGPALGGWLLQ
                     HFWWGSVFFVNVPVTAAAILAGLALIPESRDPAATPLDPAGVVLSITGLGALVYGLIE
                     APSHGWTSGTILGSLALAAVLLLAFVLVERRQRTPMMDVRLFENARFTAASLTTALVF
                     FALFGALFFLSQYLQFVLGYDTLASGMRILPVSVALIVSAPLSARLTERLGTKLVVGA
                     GMLFVTAGLALLSTASTASGYGLVAASLVLLGVGMGNVMAPATEAIMGALPPARAGVG
                     SAVSSTVRQVGGVLGVAVLGSLLSSAYATRMGATLHGVPTAVAKAA"
     gene            complement(1433..1888)
                     /locus_tag="VF995_02550"
     CDS             complement(1433..1888)
                     /locus_tag="VF995_02550"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX9342483.1"
                     /translation="MYDETSPYDTRWPHPGPAMVLVAALAGLIGGIVLGLAVSAFQGP
                     SSKASASPPTTTPAVSTTLPDRFFTVQLASFNNAGPRPDATVARYRAQHIDVAVLNSS
                     DYHGFTPGFWVIYSGTLSTRTDARAYQQKLVSDHPGLDGGVVKGPITRR"
     gene            2076..2297
                     /locus_tag="VF995_02555"
     CDS             2076..2297
                     /locus_tag="VF995_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342484.1"
                     /translation="MTVFSGVARYAHERPDIEEMARARNSARLQALERLRDYELLAGG
                     TFIERSASDCIIYGPGGLLHVYRITAELL"
     gene            2388..3539
                     /locus_tag="VF995_02560"
     CDS             2388..3539
                     /locus_tag="VF995_02560"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX9342485.1"
                     /translation="MTRIGVLTGGGDVPGLNPAIKTIAYGAIDRGHQVVGIRRGWAGL
                     LDYDLNATGPQPDCVRPLGKNDVRTIDRTGGTFLHTSRTNPGKVGPRNLPEFLKGQVQ
                     GDGPFDFTPHVVQVVERLGLDWLVAIGGDDTLSYAERLSHEGPRVVAIPKTMDNDVYG
                     TDYCIGFSTAVSRSVQFINQLRTSTGSHERIAVVELFGRNCGETSLLSAYLAGVDRAI
                     ISEVPFDPELLARYLLEDKAANPSNYAMVTVSEGASMIGGEVIESGEADAYGHRKLGG
                     IGLATGDALKRLTGQDIVYQQVGYLMRSGAPDALDLMVAVNYANMALNLIDHDSSGRM
                     VALRKGAYTSVPITTIMEGVKRVDVEDLYDEKQYRPLVRQVDGKPMFLY"
     gene            3665..4306
                     /locus_tag="VF995_02565"
     CDS             3665..4306
                     /locus_tag="VF995_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342486.1"
                     /translation="MHSLRRVVRTRPRPLRPPRGILASVRWGAPVVLILALALALAAC
                     GGKAKSPGVPSLNPTSSTSARNASSQDPYQQALAFARCMRQQGINMPDPKQNASGGIT
                     QQMGGNDTPVSKAKVDAAQKVCQKYAPQGKAGGHASQADLQKALAFARCMRQHGVNMP
                     DPKIGSDGGIGVTIGANQNDKAKIQAAQSACQKLMPGPGSSQGSGGGTNQGGN"
     gene            4306..5475
                     /locus_tag="VF995_02570"
     CDS             4306..5475
                     /locus_tag="VF995_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX9342487.1"
                     /translation="MSLDTDAAQRQQAALPDGGEVDEGRRRRRPWRVVGVLALIAVVG
                     AASWMTATRLGNNNKANAATPAPSLSTATVSRGSITSTQSVDGTLGYGSAVTVTGKLN
                     GTVTKLPTDGAIIARDHELYELDGLNPVIMLTGSRPAWRPFSSGMTDGPDVTELEQNL
                     KALGYDPNGDMTVDDHFSAATAAAIKRWQKAHNLDQTGSVPLGQVVFLPWTKVRVSSL
                     SASAGGQGMGPLMTVTSTTKQATAKLDAGSAYLVHKGMAVEIQLPGGKTSSAKISDVG
                     TVATAPNNNNPNSNGNGSSTVNVTATLTDQRAAGSLDQAPITMNITSDRRNNVMYVPV
                     TALVALAEGGFGVEVVRSDGTHQLVGVTTGLYSADGNVEVTGSGLHVGDRVVVSQ"
     gene            5526..6227
                     /locus_tag="VF995_02575"
     CDS             5526..6227
                     /locus_tag="VF995_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342488.1"
                     /translation="MTLLARPVLELRAVRKVYPGPLEVLHGIDLEIRQGELVAIIGPS
                     GSGKSTLLHIMGTLDRPSSGTVRLEGYDINRLRDRDLAALRARRIGFVFQQFFLLEGM
                     SALDNVAGGLLYSGVPLAERRRRAAGVLRRVGLGNRLYQSPAKLSGGERQRVAIARAL
                     VTRPAVVFADEPTGNLDSASGLAILDLLHELNQEGTTIVVITHDRDVAAAMNREVHIR
                     DGMIESDTMTREALV"
     gene            6224..7438
                     /locus_tag="VF995_02580"
     CDS             6224..7438
                     /locus_tag="VF995_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018585666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9342489.1"
                     /translation="MSTAALAPSRLRAADLLRLGGVGMRTRRLRATLSALGIAIGIAA
                     MVAVLGISQSSKAALLAQLDRLGTNLLTVSPGQTLGGDTAELPLAAPGMIRRIAPVQQ
                     VTSAGVTSASIYRNNHIDSANSGGLSVQAASLDLISTLGGTLHQGRWLDKATQKYPAV
                     VLGSAAAAQLGVDKVGVKVWIGGISGKAGHWFSVIGILDPNVLTPDLDRAAFVGYPIA
                     ASLLGHDGHPSTVYLRADPDSVDAVESVLGATANPQNPNEVNVSRPSDALAARIAAKG
                     AFTTLFLGLGAVALLVGGVGIANVMVIAVLERRSEIGLRRALGATKRHVWAQFLAESQ
                     VLSLLGGVAGVALGALVVAGYAANRGWAVQVPAVAVVGGIAAALVIGAIAGLYPAVRA
                     ARLSPTDALRTV"
     gene            complement(7479..8792)
                     /locus_tag="VF995_02585"
     CDS             complement(7479..8792)
                     /locus_tag="VF995_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9342490.1"
                     /translation="MSIRLRLTLVYGGLFLLAGVVLLALTFSLVRDHFAVPFRVTTVV
                     NGTSEVITGQATPFGGSVAFEGSSTQARPDAAAGPPSKEALQAVMQRARGDALRLLLV
                     NSGVALTGMAVVSFVIGWAMAGRVLRPIHRITATARRLSEQTLHERIGMRGPADELKE
                     LADTFDAMLGRLDAAFDTQRRFVANASHELRTPLAIARTEVDVTLADPDASAAELRVM
                     GERVRDATDRSERLIEGLLTLARSERRLAKREPVDLGEAAAEALAQNDAEIEALGLRV
                     SQVLGAAPAHGDHALLERMVANLVENAVRHNHRGGHIEVDSGRVGSGFAGRARMADRA
                     WVQVRVANSGPVLTEQQAASLFEPFRRLTERTGSDRGSGLGLSIVRSVAAAHGGHAQA
                     SPLPLGGLEVTVTLPADPPATPVLTATPGRPSPEPGRVTARRLEG"
     gene            complement(8806..9519)
                     /locus_tag="VF995_02590"
     CDS             complement(8806..9519)
                     /locus_tag="VF995_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9342491.1"
                     /translation="MRVLVVEDEAVLAAAITRGLRREGLAVDQSQDGADALDKLAVNR
                     YDLVVLDRDLPKVHGDEVCRQLVDPDADGNGRPSMDGVASSAGMEGRRPLILMLTASG
                     TVEDRVDGLNLGADDYLGKPFAFAELVARVHALGRRTGDPRPAVLERHGVRLDPARRE
                     ATRDGHFLHLTRKEFGVLEVLMAAGGAVVSAEDLLERVWDEHTDPFTNTVRVTLMNLR
                     RKLGRPPLIETVIGSGYRL"
     gene            complement(9682..9936)
                     /locus_tag="VF995_02595"
     CDS             complement(9682..9936)
                     /locus_tag="VF995_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342492.1"
                     /translation="MADPMLVRRLAVDLRNLSNKTLELRGAVEDYRHDMVRCADDGAF
                     DDDELRALQREIQNLWSAMDRAEAELRKASRKLSPLLWLE"
     gene            10146..10391
                     /gene="purS"
                     /locus_tag="VF995_02600"
     CDS             10146..10391
                     /gene="purS"
                     /locus_tag="VF995_02600"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX9342493.1"
                     /translation="MPRIAVDVMLKREILDPQGQAVERALPELGFEGVSDVRIGKHVE
                     LTIADGDDGEAAARRMAAALLANPVIEEFSVRVLPSS"
     gene            10425..11219
                     /locus_tag="VF995_02605"
     CDS             10425..11219
                     /locus_tag="VF995_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342494.1"
                     /translation="MPSLRLRLRRSPRLRPRPLTSTDVAAIKARPWPRLPWWLWAAMS
                     LTTVAMVVVVIGLIHGGKQASVPLGARRLPPSGDLTHTVGNYRVPALSGAAGADLRRH
                     AGCPRLASVTLAGTADQVAQLEAAAEDACGLRTTTPIERARQALDHTNAEVAFAAFQL
                     TGAESTTQLRPGQPPLVLVNADFQLRSAQAIAVELIHEGSHLADGRQPTAADELAARR
                     AELDACQRLFTPGQRPSPNPGCVDAGALLAKGDADALAELRKAGYR"
     gene            11216..12109
                     /locus_tag="VF995_02610"
     CDS             11216..12109
                     /locus_tag="VF995_02610"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9342495.1"
                     /translation="MSAQGPEPEPGPGRGAEPGRLDWPVAHGGTAVVTPERVELALVP
                     GTDPEAAFEVASVTKLLTSLAALAAVQSGRLALDQEIGDHHLPAGVTARHLLAHASGL
                     PFEEGNTRPPGTRRIYSNLGFRLLGEVVADAVGLSFEGWLRSAVLDPLGMAATSLTAR
                     RWTQADPAAGAVSTLPDLVKLARCLLDRGAPLLSQDLFGEAASVQFPGLAGLLPGIGR
                     FDPCDWGLGFELKDGKQPHWMGERRSPDSFGHFGASGCFLWVDPAVPVAAVAVSDRAF
                     DDDRWAMAVWPGWSDSVATVV"
     gene            12124..12822
                     /gene="purQ"
                     /locus_tag="VF995_02615"
     CDS             12124..12822
                     /gene="purQ"
                     /locus_tag="VF995_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX9342496.1"
                     /translation="MKRIGVITFPGSCDDRDARAAAELMGGGTVALWHSDADLRGVDA
                     LIIPGGFSYGDYLRCGAIARFSPVMGAVAGFAADGGPVLGICNGFQVLCEAGLLPGAL
                     LRNQGLRFVCREVQVIVRSTGTPWTARCQVGQRLTIPVKHGEGRYVHDDPERLAGEGQ
                     VAFTYAAGENPNGSLADIAGVRNLAGNVVGLMPHPEHAVDPLLGPTGGRALFESLLAA
                     EAEAEAEAAPVSTS"
     gene            12842..15103
                     /gene="purL"
                     /locus_tag="VF995_02620"
     CDS             12842..15103
                     /gene="purL"
                     /locus_tag="VF995_02620"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX9342497.1"
                     /translation="MSSTEGALPLHRQLGLTDEEHQRVIGSLGREPNQAELAMYSVMW
                     SEHCSYKSSKVHLRQLPVDGPRVLQGPGENAGVVDIGDGLAAVFKIESHNHPSFVEPF
                     QGAATGIGGIIRDILAMGARPLASLNSLRFGDLADPLQRRLLAGVVAGIGHYGNCVGV
                     ATVGGEVAFDPCYAGNPLVNALTLGVMPAARLLRGRAEQPGDLAVLLGAKTGRDGIGG
                     ASVLASASFEAGEEAKRPNVQVGDPFQEKLLIETCLELVERELLAGLQDLGAAGISCA
                     VSEVAARAGMGMEVDLDAVPLREPSMEPWEILISESQERMLALVHPGKLDEVTAACQR
                     WGVLATVIGTMVEGGTLTVRRSGEVVAEVPAKSLADDGPVYERPMAARPANGAATAVE
                     GERDAGEAGIGLDGTAELPPDADADPAKALLALAADPTCASKRWVYEQYDRFVGHGTV
                     AGPGSSAAVIRVPGSRRAVALATDGNGRYARLDPAAGAALAVAEAARNVACTGATPIA
                     ATKCLNFASPERPEVMGDFAAAIAGMAGACRALGTPVTGGNVSFYNESDGRPIHPTPV
                     VGVLGLLEDAGLAVPSGFPAAGLDVWLLGETRAEFGGSAWQRLAGQLEGQPPDLDLDA
                     EAALQRLLVELATRRLPASAQDLSEGGLAVALVEAVLARRIGATVELPLFEKPLVLLV
                     SESASRALLSAVPDHRAELAALAAAAGVPATRVGATGGDHLVIPAVLDLGLDELRDAY
                     EGALERRLAAAGS"
     gene            15267..15785
                     /locus_tag="VF995_02625"
     CDS             15267..15785
                     /locus_tag="VF995_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342498.1"
                     /translation="MKRGTRVGITALGLTLGLVVALGGCAKGHSTTNNSNASSGGASL
                     PTVNVSVQKFLYSGFPDTVKANQDFLVNFTNKETYPMNHEMVMVAVPSGKTKDDIVAD
                     AKKLKDKSEDNYLHFGEVPDVGTGATKVSIFNLPPGTYVIACWQSGTPQGADSGGPNH
                     LTVGMIHQFTVS"
     gene            15790..16806
                     /gene="coxB"
                     /locus_tag="VF995_02630"
     CDS             15790..16806
                     /gene="coxB"
                     /locus_tag="VF995_02630"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX9342499.1"
                     /translation="MRRRVGALVASLLCALTLSGCIRQGVSTQAQDIHDLYMRIVLLA
                     APVFVGVEVFLLYCVVRYRRRKGDNAPAPQDAGSGKILGVFFAIPVVIIAMLFPFGEQ
                     TLSFVQKQDKNPSVKINVDGFQWEWTFDYVNEGLIESGKTLVRPAVMGVPIDETIHIH
                     LQSRDVMHEFFVPSLNFMRNAIPGHPNDFDFTPTKLGTYQAQCAEFCGLWHSKMTFSV
                     LVMTRPDYVAWIGKEQAAALNVSCPPGPASTTLVAQNTSWNTDCLGMPANASLSFTVN
                     NKDSIDHSLGVFDSIKRKVKYLDTGHIPSGTRSFTIQGLRPGRYYFECIIHGPAMAGT
                     LIVR"
     gene            16823..18487
                     /gene="ctaD"
                     /locus_tag="VF995_02635"
     CDS             16823..18487
                     /gene="ctaD"
                     /locus_tag="VF995_02635"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX9342500.1"
                     /translation="MAITEVHPPALAPRPSPASRLLAWLTTTDHKKIGILYFWNSFAV
                     FAVAGLFALLMRTELAKPGMQLLTVHAYNQVFTLHGTLMIFLFIFPVLSGFGNYFVPL
                     QIGALDMAFPRINALSFWLLPVSALTILAGFLVKGGAAASGWTNYVPISIQQGAGEDL
                     WIAGLLLAGTASILGAINFIVTILRLRAPGMTMMRLPVFTWSTLATSLLVVLATPVIT
                     AGLLMLFADRRLGTVFFNPDRGGDVLLWQHVFWFFGHPEVYMLILAAWGMVSEILPVF
                     SGRPLYGYTGVVLSFLLITALSFSVWAHHMFVTGQVALPFFSVTTEMISVPTGVLFFN
                     WLATLRKGKIRFEPPMLFALGFIAMFLIGGLDGVWMASPAMDFAVHDTYWVVAHIHYV
                     LFGGTVFGVMAGMYYWFPKITGRMLNRRLGQWHFWLQLVGFNVTFFTMHLLGLKGMPR
                     RIADYAPNRGWTTLNGVATLGSYVIGISMIVFLVNVIVTLRQPRTAGDDPWGANTLEW
                     ATSSPPPAYNFDRLPRVRSNRPVYDARLAAAAAGAGSTEREEGAPA"
     gene            18484..19116
                     /locus_tag="VF995_02640"
     CDS             18484..19116
                     /locus_tag="VF995_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX9342501.1"
                     /translation="MSSADAAAPGPGPGAGAQSIDERAVASTSSSVLGMVLFVASESM
                     FFAAFFGAYVTSYVNNKIWPPNNTPAPGTTMPTIAVVALALSALLLQLAIRAGHRGEP
                     SAVNRWLALSMATAIITVILLVAGYSDVGFGLKAGIFASLFYLMTGLGLAHVAGGVVL
                     LGLVAWQGISGRLALVRYQPLQAAQVYWNFVLVLGLVIYVVFYVLTNTGG"
BASE COUNT         2845 a         6989 c         6673 g         2743 t
ORIGIN      
        1 ggccgccttg gcgacggcgg ttgggacccc gtgcagcgtg gcgcccatgc gggtggcgta
       61 ggccgacgac agcaggctgc cgagcacggc gaccccgagc acgccgccga cctggcggac
      121 ggtggagctg actgccgagc cgaccccggc gcgggcgggc ggcagggccc ccatgatcgc
      181 ctcggtggcg ggggccatga cgttgcccat gccgacgccg agcagcacca gggaggccgc
      241 taccaggccg tagccgctcg cggtcgaggc ggtcgagagc agcgccaggc cggcggtgac
      301 gaacagcatc ccggcgccga ccaccagctt ggtgccgagg cgctccgtca gccgggccga
      361 cagcggcgcg ctcacgatca gcgcgaccga caccggcagg atgcgcatgc cggaggcgag
      421 ggtgtcgtag ccaagcacga actgcaggta ctggctgagg aagaacagcg cgccgaagag
      481 ggcgaagaac accagggcgg tggtcaggct ggcggcggtg aagcgggcgt tctcgaacag
      541 gcgcacgtcc atcatcgggg tgcgctggcg ccgctcgacc agcacgaagg cgagcagcag
      601 gaccgcggcc agcgccagcg agccgaggat cgtgccgctg gtccagccgt gcgacggggc
      661 ctcgatcagg ccgtagacca gggcgccgag cccggtgatc gacagcacca cgccagcggg
      721 gtcgagcggg gtggcggccg ggtcgcgcga ctcggggatc agcgccaggc cggcgaggat
      781 cgccgccgcg gtgaccggga cgttgacgaa gaacaccgag ccccaccaga agtgctgcag
      841 cagccagccg ccgagcgccg ggccgatggc gatgccgagc ccggagacgc cggcccagat
      901 gccgatggcg cggccccgct cgctggccgg gaagacgttg gtgacgatcg acagcgtcga
      961 gggcatgatc agcgcggcgc cgaggcccat cacggcgcgc caggcgatca gcgtcccggc
     1021 gctgccggcg aaggccgaga gcccggaggc ggtgccgaag agcagcatcc cggcggcgag
     1081 cacgcggcgc cgcccgaagc ggtcgccgag cgccccggcg gtgagcagca tcccggcgaa
     1141 gaccagcgcg taggagtcga ccatccactg cagctggctg ccactggcgt gcaggtcgcg
     1201 gaccagcgtg ggcagcgcca cgttgaggat ggtgttgtcg agcccgatga ccagcaagct
     1261 caggcacagc accccgagcg tccaccagcg cctcgggtag agcgcctctg gcattggagc
     1321 ccccttccat ggaacggaac cgtatcgttt cgtaatctgg aagcaggcta gcacgcccta
     1381 cgaggaaacg caacggtctc gtatcgttat ttggggaggg cgagcgggcc gcctatcgcc
     1441 gggtgatcgg gcccttgacg acgccgccgt ccagccctgg atggtcgctg accagcttct
     1501 gctggtaggc cctggcgtcc gtcctggtgc tgagggtgcc gctgtagatc acccagaagc
     1561 cgggggtgaa gccgtggtag tcggagctgt tcagcacggc cacgtcgatg tgctgggcgc
     1621 ggtaccgggc gacggtggcg tcggggcgcg gcccggcgtt gttgaagctg gcgagctgca
     1681 ccgtgaagaa gcgatcaggc agcgtggtgc tcaccgccgg cgtcgtcgtc ggggggctgg
     1741 cactcgcctt gctggatgga ccttggaagg cactcacggc caagcccagg acgatcccgc
     1801 cgatcaggcc ggcgagcgcg gcgaccagca ccatggccgg cccggggtgg ggccagcggg
     1861 tgtcgtaggg gctggtctcg tcgtacatgg gcatacgcta gccgccgtat tcttgatcct
     1921 caagtctgct cggcgttctc gaatgatcat cttctcgccc gctcgttacc agggttgctt
     1981 gcccggtaaa cgaccggtaa gcgataaggt agagcggtgc cggccgaccc agcggccgct
     2041 ggtcccactc ggtccatccg gaggtcgcgc aagcaatgac cgtcttctca ggcgtcgccc
     2101 gctacgctca cgagcgtccg gacatcgagg agatggcccg ggccaggaac tcggcccggc
     2161 tgcaagcgct ggagcggctg cgggactacg agctgctggc cggtgggacc ttcatcgagc
     2221 ggtccgccag cgactgcatc atctacgggc ccggcgggct gctgcacgtc taccgcatca
     2281 ccgccgagct gctgtagctg gtctaataac ggcgacatac ctgcctgaac gagacctgcc
     2341 tgaacgggct cggcctacaa gctccatcgc gcggaggctt caaccacatg actcgcatcg
     2401 gcgtgctcac cggcggggga gacgtccccg gactcaaccc tgccatcaag acgatcgcct
     2461 atggcgcgat cgaccgtggt caccaggtcg tcggcatccg ccgcggctgg gccggcctgc
     2521 tcgactacga cctgaacgcg accggcccgc agccggactg cgtgcggccg ctcggcaaga
     2581 atgacgtccg caccatcgac cgcaccggcg gcaccttcct gcacaccagc cgcaccaacc
     2641 ccggcaaggt cggcccgcgc aacctgcccg aattcctcaa gggccaggtc cagggcgacg
     2701 gccccttcga cttcaccccc cacgtggtgc aggtcgtcga gcggctcggc ctcgactggc
     2761 tggtggcgat cggtggcgac gacaccctct cctacgccga gcggctctcg cacgagggcc
     2821 cgcgagtggt cgccatcccc aagaccatgg acaacgacgt ctacgggacc gactactgca
     2881 tcggcttctc caccgccgtc tcccgcagcg tccagttcat caaccagctg cgcacctcca
     2941 ccggctcgca cgagcgcatc gcggtggtgg agctgttcgg ccgcaactgc ggcgagacct
     3001 cgctcctgtc ggcctacctc gccggcgtgg accgggcgat catctccgag gtccccttcg
     3061 acccggagct gctggcccgc tacctgcttg aggacaaggc cgccaacccg tcgaactacg
     3121 ccatggtgac ggtcagcgag ggcgccagca tgatcggcgg cgaggtgatc gagtccggcg
     3181 aggccgacgc ctatggccac cgcaagctcg gcggcatcgg cctcgccacg ggcgacgcgc
     3241 tgaaacggct caccggccag gacatcgtct accagcaggt cggctacctg atgcgctccg
     3301 gcgcgcccga cgcgctggac ctgatggtgg cggtcaacta cgccaacatg gccctcaacc
     3361 tgatcgacca cgactcctcc ggccgcatgg tggcgctgcg caagggcgcc tacacctcgg
     3421 tgccgatcac caccatcatg gagggcgtca agcgcgtcga cgtcgaggac ctctacgacg
     3481 agaagcagta ccgccccctg gttcgccagg tcgacggcaa accgatgttc ctgtattgac
     3541 ctggttggcc ggtgtcgcat ccgcgtaacg tggacgtttt acgcagatga tcgtgccatc
     3601 cgagtagacc tcctggtgac agccgcgacc cgcgcggccc gatccaggag gttcctcgtt
     3661 gttcatgcat tctctcaggc gcgtcgtgcg cacgcggcca cggccgctgc gcccaccgcg
     3721 cggcatcctc gcctccgtcc gctggggagc gccggtggtc ctcatcctgg cgctcgcgct
     3781 ggccctggcg gcctgcggcg gcaaagccaa gagtcccggc gtgccgagcc tcaacccgac
     3841 gtccagcacc tcggcccgga acgccagcag tcaggacccc taccagcagg ctctcgcctt
     3901 cgcccgctgc atgcgccagc agggcatcaa catgcccgac ccgaagcaga acgccagcgg
     3961 gggcatcacc cagcagatgg gcggcaacga cacccccgtc tccaaggcga aggtcgacgc
     4021 ggcgcagaag gtctgccaga agtacgcccc gcagggtaaa gccggcggcc atgccagcca
     4081 ggcggacctc cagaaggccc tcgccttcgc ccgctgcatg cgccagcacg gggtcaacat
     4141 gcccgacccc aagatcggca gcgacggcgg gatcggggtg accataggcg cgaaccagaa
     4201 cgacaaggcc aagatccagg cggcacagtc ggcctgccag aagctcatgc cagggccagg
     4261 atccagccaa gggtcgggcg gcggcaccaa ccaggggggt aactgatgag cctcgacacc
     4321 gacgcggccc agcggcagca ggccgctctc cccgacgggg gcgaggtgga cgagggccgc
     4381 aggcgccggc gcccgtggcg ggtggtcggc gtcctcgccc tgatcgcggt ggtcggggcg
     4441 gcctcctgga tgaccgccac acggctcggc aacaacaaca aggccaatgc ggccacgccg
     4501 gcgccgtccc tgtccaccgc caccgtctcg cgggggagca tcacctcgac ccagtcggtc
     4561 gacgggaccc tcggctacgg cagcgccgtc acggtgacgg gcaagctgaa cggcaccgtc
     4621 accaagctgc caaccgatgg cgccatcatc gcccgcgacc acgagctgta cgagctggac
     4681 gggctcaacc cggtgatcat gctgaccggc tcccggcccg cctggcgacc gttcagcagc
     4741 ggcatgaccg atggtcccga cgtcaccgaa ctggagcaga acctcaaggc gctcggctac
     4801 gaccccaacg gcgacatgac cgtcgacgac cacttcagcg cggcgaccgc cgccgcgatc
     4861 aagcgctggc agaaggccca caacctcgac cagaccggca gcgtcccgct cggtcaggtg
     4921 gtgttcctgc cgtggaccaa ggtgcgggtg tcgtcgctga gcgcctcggc cggcggccag
     4981 gggatggggc cgctcatgac cgtcacctcg accaccaagc aggcgaccgc caagctcgac
     5041 gcgggctcgg cctacctcgt tcacaagggc atggccgtgg agatccagct gcccggcggc
     5101 aagaccagca gcgccaagat cagcgacgtc ggcaccgtcg ccaccgcgcc caacaacaac
     5161 aaccccaaca gcaacggcaa cggcagctcg acggtgaacg tcaccgccac gctgaccgac
     5221 cagcgcgccg ccggcagcct cgaccaggcg cccatcacca tgaacatcac cagcgacagg
     5281 cgcaacaacg tcatgtacgt gccggtgacc gcgctggtcg ccctcgcgga gggcggcttc
     5341 ggggtcgagg tcgtccgctc cgacggcacc caccagctgg tcggcgtgac caccgggctc
     5401 tactcggccg acggcaacgt ggaggtcacc ggcagcggcc tgcacgtcgg cgaccgcgtg
     5461 gtggtgtccc aatgagcgac gaggtgagcg cggccggggc ggcggacaac ggcgacggtg
     5521 cgtcgatgac gctcctggcg cggccggtcc tggagctccg ggcggtccgc aaggtctacc
     5581 ccggcccgct ggaggtcctg cacgggatcg acctggagat ccgccagggc gagctggtcg
     5641 cgatcatcgg gccgtccggg tcggggaaat cgacgctgct ccacatcatg ggcacgctcg
     5701 accggccaag ctcgggcacg gttcgcctgg aggggtatga catcaaccgg ctgcgcgacc
     5761 gggatctcgc ggcgctgcgg gcgcggcgta tcggcttcgt cttccagcag ttcttcctgc
     5821 tggagggcat gagcgcgctc gacaacgtgg ccggcggcct gctctacagc ggcgtgccgc
     5881 tggccgagcg gcgccgccgc gccgccgggg tgctgcgccg cgtcggcctc ggcaaccgcc
     5941 tgtaccagtc gccggccaag ctctcgggtg gcgagcggca gcgggtggcg atcgcccgcg
     6001 cgctcgtcac caggccagcg gtggtgttcg ccgacgagcc caccggcaac ctcgactccg
     6061 cctctggcct ggccatcctc gacctgctcc acgaactgaa ccaggaaggc accaccatcg
     6121 tggtgatcac ccacgaccgc gacgtcgccg cggcgatgaa ccgggaggtc cacatcaggg
     6181 acggcatgat cgaatcagac acgatgacga gggaggcatt ggtatgagta ccgcggcgct
     6241 cgcgcccagc cggttgcggg ccgccgacct gctgcgactc ggcggggtgg gcatgcgcac
     6301 gcgcaggctg cgcgccaccc tgtccgcgct cgggatcgcc atcggcatcg ccgcgatggt
     6361 ggcggtgctc ggcatctcgc agtccagcaa ggcggccctg ctcgcccagc tcgaccggct
     6421 cggcaccaac ctcctgacgg tgagcccggg gcagaccctc ggcggcgaca ccgccgagct
     6481 gccgctggcc gcaccgggaa tgatccgccg tatcgccccg gtgcagcagg tcacctcggc
     6541 tggggtaacg tcggccagca tctaccgcaa caaccacatc gactcggcca acagcggcgg
     6601 cctcagcgtg caggcggcca gcctcgacct gatcagcacc ctcgggggca ccctgcacca
     6661 gggccgctgg ctcgacaagg ccacccagaa gtacccggcg gtggtgctcg gcagcgccgc
     6721 cgcggcccag ctcggcgtgg acaaggtcgg cgtgaaggtc tggatcggcg gcatcagcgg
     6781 caaagccggg cactggttct cggtgatcgg catcctcgat ccgaacgtgc tgacccccga
     6841 cctggaccgg gccgccttcg tcggctaccc gatcgcggcc agcctgctcg gccacgacgg
     6901 ccacccgagc acggtgtacc tgcgcgccga cccggacagc gtcgacgccg tggagagcgt
     6961 gctgggcgcc accgccaacc cgcagaaccc caacgaggtg aacgtcagcc gcccgtccga
     7021 cgcactggcg gcgcggatcg cggccaaggg cgccttcacc acgctgttcc tcggcctcgg
     7081 cgcggtcgcg ctgctggtcg gcggggtcgg catcgccaac gtgatggtga tcgcggtgct
     7141 ggagcgccgc tccgagatcg gcctgcgccg cgcgctcggc gccaccaaac ggcacgtgtg
     7201 ggcgcagttc ctggccgagt cgcaggtgct cagcctgctc ggcggcgtcg ccggggtcgc
     7261 tctcggcgcc ctggtggtgg cgggctacgc cgccaaccgc ggctgggcgg tgcaggtccc
     7321 agcggtcgcc gtggtcggcg gcatcgccgc cgcgctggtg atcggcgcga tcgccggcct
     7381 ctacccggcc gtgcgtgccg ctcggctctc accgaccgac gccctccgga cggtgtgagg
     7441 tgtgagcaca cggcacggcc aaccccacgg cccggcggct acccctccag ccgccgggcc
     7501 gtgacacgtc cgggttcagg gcttggtcgt ccaggggtcg ccgtgagcac cggcgtggcc
     7561 ggtgggtccg ctgggagggt cacggtcacc tccaggccac cgagggggag cgggctggcc
     7621 tgggcgtggc cgccgtgggc ggcggcgacc gagcggacga tcgacaggcc gaggccgctg
     7681 ccccggtcag agccggtgcg ctcggtgagg cgccggaacg gctcgaacag ggaggcggcc
     7741 tgttgctcgg tgagcaccgg gccgctgttc gccacccgga cctgcaccca ggcgcggtcc
     7801 gccatccggg cgcggccggc gaagccgctg cccacccggc cgctgtcgac ctcgatgtgg
     7861 ccgcctcggt ggttgtggcg cacggcgttc tcgaccagat tcgccaccat gcgctccagc
     7921 agggcatggt cgccgtgggc cggcgccgcg ccgagcacct ggctgacccg caggccgagc
     7981 gcctcgatct ccgcgtcgtt ctgggcgagc gcctcggcgg cggcctcgcc caggtcgacc
     8041 ggctcgcgct tggccaggcg gcgctcgctg cgcgccagcg tcagcagccc ctcgatcagg
     8101 cgctcgctgc gatcggtggc gtcgcgcacc cgctcgccca tgacgcgcag ctcggccgcg
     8161 ctcgcgtccg ggtcggccag ggtgacgtcc acctcggtcc gggcgatcgc cagcggggtg
     8221 cgcagctcgt gggaggcgtt ggcgacgaag cggcgctggg tgtcgaaggc ggcgtcgagg
     8281 cgcccgagca tggcgtcgaa ggtgtcggcc agctccttca gctcgtcggc ggggccgcgc
     8341 atgccgatgc gctcgtgcag ggtctgctcg gagaggcgcc tggcggtggc ggtgatccgg
     8401 tggatcgggc gcagcacccg cccggccatg gcccagccga tcacgaacga gacgaccgcc
     8461 atgccggtga gcgccacccc ggaattgacc agcaggaggc ggagcgcatc cccgcgtgcc
     8521 cgctgcatca cggcctgcag ggcctccttg ctgggcgggc cggccgcggc atcaggacgg
     8581 gcctgtgtcg aggagccctc aaatgcgacc gagccgccga acggggtcgc ctgcccagtg
     8641 atcacctcgc tggtgccgtt caccacggtc gtgaccctga acggcacggc gaaatggtca
     8701 cgcaccagcg agaacgtcag cgccagcaag acgaccccgg cgagcaggaa cagccctccg
     8761 tagaccaggg tcagccgcag ccggatgctc atcggcccgg tccggtcaca gccggtaccc
     8821 ggagccgatc acggtctcga tcagcggcgg gcggccgagc ttgcggcgca ggttcatgag
     8881 ggtcacccgg acggtgttgg tgaacgggtc ggtgtgctcg tcccagaccc gttccagcag
     8941 gtcctcggcg ctcaccaccg cgccgccggc ggccatcagg acctcaagga cgccgaactc
     9001 cttgcgggtc aggtgcagga agtggccgtc gcgggtggct tcgcgccggg ccgggtcgag
     9061 gcggacgccg tggcgctcca gcaccgccgg gcgcgggtcg ccagtgcgcc ggccgagcgc
     9121 gtggacccgc gccaccagct cggcgaaggc gaacggcttg ccgaggtagt cgtcggcgcc
     9181 aaggttgagg ccgtcgaccc ggtcctccac ggtgccggag gcggtcagca tcaggatcag
     9241 cggccgccgg ccttccatgc cggcggacga agcgacgcca tccatggaag gccggccgtt
     9301 gccgtccgcg tcgggatcga ccagctggcg gcagacctcg tcgccgtgga ccttgggcag
     9361 gtcccggtcg aggaccacca ggtcgtagcg gttcacggcc agcttgtcga gggcatccgc
     9421 gccgtcctgg gactggtcga cggccagccc ctcccggcgc agcccccggg tgatcgccgc
     9481 cgccagcacg gcctcatcct ccactacgag cacgcgcatc cgcccgcgag cacctcccca
     9541 tctcgtccac gattcgaccg gtcccgcggg cgaggaccgg tcggggtcgt tgaccgctcg
     9601 ccggcgggac cggcgagacc agggtgccgc aggacgccta acgcctgcgt aaggcgacgc
     9661 accaggaggc tttggggcgg gctactcgag ccagagcagc ggcgacagtt tccgggaggc
     9721 tttgcggagc tccgcctcgg cccggtccat ggcgctccag aggttctgga tctcgcgctg
     9781 gagggcgcgc agctcgtcgt cgtcgaaggc gccgtcgtcg gcgcagcgga ccatgtcgtg
     9841 gcggtaatcc tcgaccgcgc cgcgcagctc gagggtcttg ttcgagagat tgcgcagatc
     9901 gactgccagc cgccgaacga gcatggggtc cgccatcaat cgatcaacct cccgaggctc
     9961 ccggtcctag atgaagccaa tggagcatag cgccatgaag ccgaaaagaa aggcacaaga
    10021 acggatgaat ggatgaaaag ttgctgagaa gtgtttgcca cccctgaggg gtccgaacgg
    10081 cggggcgacc gggcccaatg atagccttcg gtaccgactt cggagggctc agagaggatg
    10141 aacccgtgcc tcggatcgcc gtcgacgtga tgctgaaacg cgagatcctg gacccgcagg
    10201 gccaggcggt cgagcgtgcc ctgccggagt tgggcttcga gggcgtcagc gacgtccgca
    10261 tcggcaagca cgtcgagctc acgatcgctg acggcgacga cggtgaggcg gccgcccgcc
    10321 gcatggccgc ggcgcttctc gccaacccgg tcatcgagga gttctcggtc cgggtcctgc
    10381 cctcgtcctg atcttctggc cgctgctccg tggaccgctc actcgtgcca agcctccgcc
    10441 tccgcctccg ccgctctccc cggttgcggc cccggccgct cacctccacc gacgtggccg
    10501 ccatcaaagc ccgcccctgg ccgcgcctgc cctggtggct gtgggccgcc atgtcgctga
    10561 ccaccgtggc catggtggtg gttgtgatcg gcctgatcca cggtggcaag caggcgtcag
    10621 tgccgctcgg cgcccgccgc ctgccgccga gcggcgacct gacccatacg gtcggcaact
    10681 accgggtccc ggcgctcagc ggcgctgccg gcgccgacct gcggcgccac gccgggtgcc
    10741 cgcggctcgc cagcgtcacc ctggcgggca ccgcggacca ggtcgcccag ctggaggccg
    10801 ccgccgagga cgcctgcggg ctgcgcacga ccacgccgat cgagcgggcc cggcaggcgc
    10861 tcgaccacac caacgccgag gtcgcgttcg ccgcgtttca attgaccggg gccgagtcca
    10921 ccacccagct gcggccgggc cagccgccgc tggtgctggt caacgcggac ttccagctcc
    10981 gctccgcgca ggcgatcgcg gtggagctga tccacgaggg cagccacctc gccgacggcc
    11041 gccagccgac cgccgccgac gagctggccg cccgccgcgc cgagctggac gcctgccagc
    11101 ggctcttcac ccccggccag cggccgtccc cgaatcccgg ctgcgtggac gcgggcgcgc
    11161 tgctggccaa gggcgacgcc gacgccctcg ccgagcttcg caaggccggc taccggtgag
    11221 cgcgcagggg ccggaaccgg agccggggcc agggcgtggg gcggagccgg ggcggctcga
    11281 ctggccggtg gcgcatggcg ggaccgccgt ggtgaccccg gagcgggtcg agctcgcgct
    11341 ggttcccggg acggatccgg aggccgcttt cgaggtggcc tcggtgacca agctgctcac
    11401 ctcgctggcg gcgctggcgg cggtgcagtc cgggcggctc gccctcgacc aggaaatcgg
    11461 cgaccaccac ctcccggcgg gcgtgaccgc ccggcacctg ctcgcccacg cctctgggct
    11521 gccgttcgag gaggggaaca cgcgcccgcc cgggacgcgg cggatctact ccaacctcgg
    11581 tttccgcctg ctcggcgagg tggtcgccga cgcggtcggg ctgagcttcg agggctggct
    11641 gcggagcgcc gtcctcgacc cgctcggcat ggccgcgacc agcctgaccg cccgccggtg
    11701 gacccaggcg gacccggccg ccggagcggt cagcaccctc cccgacctgg tcaagctggc
    11761 ccgctgcctg ctcgatcggg gcgccccgct gctcagccag gacctcttcg gcgaggccgc
    11821 cagcgtccag ttccccgggc tcgccgggct gctccccggg atcgggcgct tcgacccctg
    11881 cgactggggc ctcggcttcg agctgaagga cggcaagcag ccccactgga tgggggagcg
    11941 ccgctccccg gacagcttcg gccacttcgg cgccagcggt tgcttcctct gggtcgatcc
    12001 cgccgtgccc gtcgccgccg tcgccgtcag cgaccgtgcc ttcgacgacg acaggtgggc
    12061 gatggccgtc tggcccggat ggtctgacag cgtggcgacc gtggtctgat agcgtgtggc
    12121 cgcatgaaac ggatcggggt catcaccttc ccgggctcct gcgacgaccg ggacgcccgt
    12181 gccgccgccg agctgatggg cggtggcacc gtcgcgctgt ggcacagcga cgcggacctg
    12241 cgcggggtcg acgcgctgat catcccgggc ggcttttcct acggcgacta cctgcgttgc
    12301 ggcgcgatcg cgcggttctc gccggtgatg ggcgcggtcg ccggcttcgc cgccgacggc
    12361 ggcccggtgc tcgggatctg caacggcttc caggtgctgt gcgaggcggg cctgcttccc
    12421 ggcgcgctgc tccgcaacca gggcctccgc ttcgtctgcc gcgaggtcca ggtcatcgtc
    12481 cgctcgaccg gaacgccgtg gaccgcccgc tgccaggtgg gccagcgcct gaccattccc
    12541 gtgaagcacg gcgagggccg ctacgtccac gacgaccccg agcgcctggc cggcgagggc
    12601 caggtggcgt tcacctacgc ggcaggggag aaccccaacg gctccctcgc cgacatcgcc
    12661 ggggtccgga acctcgccgg caacgtggtc ggcctgatgc cccatcccga gcatgccgtg
    12721 gacccgctgc tcggcccgac cggcggccgc gccctcttcg agagcctgct ggcggccgaa
    12781 gccgaagccg aggccgaggc ggcgccagtg tccacgtcct gatcgtatgg agggggagca
    12841 cgtgtccagc accgaggggg ctctgccgct gcaccggcag cttggcctga ccgacgagga
    12901 gcaccagcgc gtcatcggga gcctcggccg cgagccgaac caggccgagc tggcgatgta
    12961 ctcggtgatg tggtcggagc actgctccta caagtcctcc aaggtccacc tgcgccagct
    13021 ccccgtcgac gggccccggg tgctgcaggg gcccggcgag aacgccgggg tggtcgacat
    13081 cggcgacggc ctcgccgcgg tcttcaagat cgagtcccac aaccacccgt cgttcgtgga
    13141 gccgttccag ggtgccgcca ccgggatcgg ggggatcatc cgcgacatcc tcgcgatggg
    13201 cgcgcgaccg ctggccagcc tcaactcact ccgcttcggg gacctggccg acccgctgca
    13261 gcggcgcctg ctggccgggg tggtcgccgg gatcggccac tacggcaact gcgtcggcgt
    13321 cgccaccgtc gggggcgagg tcgccttcga cccctgctac gccggcaacc cgctggtcaa
    13381 cgcgctcacc ctgggggtga tgccggccgc ccggctgctc cgcggccggg ccgagcagcc
    13441 cggcgacctg gcggtgctgc tcggcgccaa gaccggccgc gacggcatcg gcggcgccag
    13501 cgtgctcgcc tccgcctcct tcgaggccgg ggaggaggcg aagcgcccga acgtccaggt
    13561 cggcgacccg ttccaggaga agctgctgat cgagacctgc ctggagctgg tcgagcggga
    13621 gctgctcgcc ggcctccagg acctgggcgc ggcggggatc tcctgcgcgg tcagcgaggt
    13681 ggccgcccgt gccgggatgg gcatggaggt cgacctcgac gcggtgccgc tgcgcgaacc
    13741 ctccatggag ccgtgggaga tcctgatctc cgagtcgcag gagcgcatgc tggcgctggt
    13801 ccaccccggc aagctcgacg aggtcacggc ggcctgccag cgctggggcg tgctcgccac
    13861 cgtcatcggg acgatggtgg agggcggcac cctgacggtc cgccgcagcg gcgaggtggt
    13921 cgccgaagtg cccgccaaga gcctcgccga cgacggcccg gtctatgagc gccccatggc
    13981 agcgcgaccg gcgaacggcg cagctactgc ggtggagggc gagcgggacg cgggcgaagc
    14041 cggcatcggg ctcgacggca ccgccgagct gccgccggac gccgacgccg acccggccaa
    14101 ggcgctgctg gcgctcgccg ccgacccgac ctgcgcctcc aagcgctggg tctacgagca
    14161 gtacgaccgc ttcgtcgggc acggcaccgt cgccggacca gggtcgagcg cggccgtgat
    14221 ccgggtgccc gggagccgcc gcgcggtcgc cctggccacc gacggcaacg gccgctacgc
    14281 ccgcctcgac ccggccgccg gtgccgccct ggccgtggcc gaggcggccc gcaacgtcgc
    14341 ctgcaccggc gcgaccccga tcgccgccac caagtgcctg aacttcgcca gccccgagcg
    14401 ccccgaggtg atgggcgact tcgccgccgc gatcgccggc atggccggcg cctgccgcgc
    14461 gctcggcact ccggtcaccg gcggcaacgt gagcttctac aacgagtccg acggccgccc
    14521 gatccacccg acacccgtcg tcggggtgct cggcctgctg gaggacgccg gcctggcggt
    14581 cccgagcggc ttccccgccg ccggcctgga cgtctggctg ctgggcgaga cccgcgcgga
    14641 gttcggcggc tcggcctggc agcgcctggc cggccagctg gagggccagc cgcccgacct
    14701 cgacctggac gccgaggcgg ccctgcagcg gctgctggtc gagctggcaa cccgccgcct
    14761 gcccgcgagc gcccaggacc tgtccgaggg cggcctcgcg gtggccttgg tcgaggcggt
    14821 cctcgcgcgc cgcatcggcg ccaccgtcga gctgcccctc ttcgagaagc cgctggtgct
    14881 gctggtcagc gagtcggcct cgcgggcgct gctcagcgcc gtccccgacc accgcgccga
    14941 gctggccgcc ctcgccgccg cggccggggt gcccgccacc cgcgtcggcg cgaccggcgg
    15001 cgaccacctg gtcatccccg ccgtcctcga cctcggcctg gacgagctgc gcgacgccta
    15061 cgagggcgcg ctggagcgaa ggctggcggc agccgggtca tgaggcccgc tcgatgaagg
    15121 attcatgaca ggactgtcat ctgtgggcgc ggctgacaag ggacgtccca gcgcctgaat
    15181 gacagtcctg tcaccgttgc tgacctccgc acttcggcgc cgtagcgttg gcccaccgtt
    15241 acctctcgac gatggaggag cctgcgatga aacgcgggac acgggtggga atcacggccc
    15301 tggggctgac cctcgggctc gtggtcgccc tcggcggctg cgccaagggc cattcgacca
    15361 ccaacaacag caacgcttcc agtggcggcg cctcgctgcc gactgtcaac gtctcggtcc
    15421 agaagttcct ctacagcggc ttcccggaca cggtaaaggc gaaccaggac ttcctggtca
    15481 acttcaccaa caaagagacc tacccgatga accacgagat ggtcatggtc gccgtcccct
    15541 ccggcaagac caaggacgac atcgtggccg acgccaagaa gctgaaggac aagtccgagg
    15601 acaactacct tcacttcggc gaagtccccg acgtcggcac cggcgccacc aaggtgagca
    15661 tcttcaacct gccgccgggc acctatgtga tcgcctgctg gcagagcggc accccgcagg
    15721 gtgcggactc cggcggcccg aaccacctca cggtcggcat gatccaccag ttcacggtga
    15781 gctgacgcga tgcgccggcg tgttggcgcc ctggtcgcgt ccctgctctg tgccctgacg
    15841 ctcagtgggt gcatccgcca gggcgtcagc acccaggcgc aggacatcca cgatctgtac
    15901 atgcggatcg tgctgctggc cgcgccggtg ttcgtggggg tcgaggtgtt cctcctgtat
    15961 tgcgtcgtgc gctaccgcag gcgcaagggt gacaacgccc cggcccccca ggacgccggc
    16021 agcggcaaga tcctcggggt gttcttcgcc atcccggtgg tgatcatcgc gatgctgttc
    16081 cccttcgggg agcagaccct cagcttcgtc cagaagcagg acaagaaccc cagcgtcaag
    16141 atcaacgtcg acggcttcca gtgggagtgg accttcgact acgtcaacga gggcctgatc
    16201 gagagcggca agacgctggt gcgcccggcc gtgatggggg tcccgatcga cgagacgatc
    16261 cacatccacc tgcagtcccg cgacgtgatg cacgagttct tcgtgccgtc cctgaacttc
    16321 atgcgcaacg ccatccccgg ccatcccaac gacttcgact tcacgcccac caagctcggc
    16381 acctaccagg cgcagtgcgc cgagttctgc gggctgtggc actcgaagat gaccttcagt
    16441 gtcctggtga tgacccggcc cgactacgtc gcctggatcg gcaaggagca ggcagccgcc
    16501 ctcaacgtca gctgcccgcc tgggccggcc tcgaccacgc tggtcgccca gaacacctcc
    16561 tggaacaccg actgcctggg catgccggcc aacgcctcgc tcagcttcac ggtcaacaac
    16621 aaggactcga tcgaccacag cctcggggtg ttcgactcga tcaagcgcaa ggtcaagtac
    16681 ctggacaccg ggcatatccc cagcgggacc cggtccttca cgatccaggg gctgcggccc
    16741 ggccgctact acttcgagtg catcatccac ggcccggcca tggccggcac cctgatcgtc
    16801 agataggagg gaccagcttc acatggcgat cactgaggta cacccgccgg cgctggctcc
    16861 ccggccgtcc ccggcctccc ggctgctggc ctggctgacc accaccgacc acaagaagat
    16921 cgggatcctg tacttctgga actcgttcgc ggtcttcgcg gtcgccgggc tgttcgcgct
    16981 gctgatgcgc accgagctgg ccaagccagg catgcagctg ctcaccgtgc acgcctacaa
    17041 ccaggtcttc acgctgcacg gcacgctgat gatcttcctg ttcatcttcc cggtgctcag
    17101 tggcttcggg aactacttcg tgccgctgca gatcggcgcc ctggacatgg ccttcccgcg
    17161 gatcaacgcg ctgtcgttct ggctgctgcc ggtgtcggcg ctgacgatcc tggccgggtt
    17221 cctggtcaag ggcggcgcgg cggcctccgg ctggaccaac tacgtgccga tctcgatcca
    17281 gcagggagcc ggcgaggacc tgtggatcgc cgggctgctg ctggccggca ccgcgtcgat
    17341 cctcggggcg atcaacttca tcgtgaccat cctgcggttg cgcgccccgg gcatgaccat
    17401 gatgcggctg ccggtgttca cctggagcac cctggcgacc tcgctgctgg tggtgctggc
    17461 gaccccggtg atcaccgccg ggctgctgat gctgttcgcc gaccggcgcc tcggcacggt
    17521 gttcttcaac cccgaccggg gcggcgacgt gctgctgtgg cagcacgtct tctggttctt
    17581 cgggcatccc gaggtctaca tgctgatcct ggcggcctgg gggatggtca gcgagatcct
    17641 gccggtgttc tcgggccggc cgctgtacgg ctacaccggc gtggtgctgt cgttcctgct
    17701 gatcaccgca ctgtcgttca gcgtctgggc gcaccacatg ttcgtcaccg gccaggtggc
    17761 gctgccgttc ttctcggtga ccaccgagat gatctcggtc ccgaccgggg tgctgttctt
    17821 caactggctc gccaccttgc ggaagggcaa gatccgcttc gagccgccga tgctgttcgc
    17881 cctcggcttc atcgccatgt tcctgatcgg cggccttgac ggcgtctgga tggcgtcccc
    17941 ggcgatggac ttcgccgtcc acgacaccta ctgggtagtc gcccacatcc actacgtgct
    18001 gttcggcggc accgtgttcg gcgtgatggc gggcatgtac tactggttcc cgaagatcac
    18061 cggacggatg ctcaaccgcc gcctcgggca gtggcacttc tggctgcagc tcgtcggctt
    18121 caacgtgacc ttcttcacca tgcacctcct ggggttgaag ggcatgccgc gccgcatcgc
    18181 cgactacgcg cccaaccgcg gctggacgac cctcaacggc gtcgccaccc tcgggtccta
    18241 cgtcatcggc atctcgatga tcgtgttcct ggtcaacgtg atcgtcaccc tgcgccagcc
    18301 gcgcaccgcc ggcgacgacc cctggggggc caacaccctg gagtgggcga ccagctcgcc
    18361 gccgccggcc tacaacttcg accggctgcc acgggtccgc tccaaccggc cggtgtacga
    18421 cgcccggctc gccgcggcgg ccgctggcgc tggttccact gagcgtgagg agggagcgcc
    18481 agcatgagca gcgccgacgc cgcagccccc ggcccgggtc caggcgcggg cgcccagagc
    18541 atcgacgagc gggcggtcgc cagcacctcc agctccgtgc tcggcatggt gttgttcgtc
    18601 gcctccgagt cgatgttctt cgccgccttc ttcggcgcct acgtcacctc ctacgtcaac
    18661 aacaagatct ggccgcccaa taacactccc gcgccaggga ccaccatgcc gaccatcgcg
    18721 gtggtggcgc tggcgctctc ggcgctgctg ttgcagctcg ccatccgcgc tgggcatcgc
    18781 ggcgagccca gcgcggtcaa ccgttggctc gcgctcagca tggccaccgc gatcatcacg
    18841 gtgatcctgc tggtcgccgg ctacagcgac gtcggcttcg gcctcaaggc cgggatcttc
    18901 gcctcgctgt tctacctgat gaccggcctc ggcctggccc acgtggccgg cggggtggtg
    18961 ctcctcggcc tggtcgcctg gcaggggatc agcggccgcc tcgcgctggt ccgctaccag
    19021 ccgctgcagg ccgcccaggt gtactggaac ttcgtgctgg tcctcggcct ggtcatctac
    19081 gtggtcttct acgtgctcac caacaccgga gggtagggtc gacgtgaagc tctacgcgcg
    19141 ggtcgcgctc ggcatcggcg tcttcctgtt cgtcgccggc ctggtctaca actcgctcta
    19201 caaggaacgc atcggcggcg tggagatgct gatcgccgct ggcggcttca
//
LOCUS       DASWDC010000082        10360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_685556, whole genome shotgun sequence.
ACCESSION   DASWDC010000082 DASWDC010000000
VERSION     DASWDC010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685556"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VF995_02645"
     CDS             complement(<1..467)
                     /locus_tag="VF995_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX9342502.1"
                     /translation="MTTDLRSDTMTKPTPAMRQAMADAEVGDDQWREDPTTKRLEAAV
                     AERFGREAALFVPSGHMGNQICLRLGAAPGSECLAHEDNHIFWYENAAGSLLSGIQFH
                     AMTGPRGLLDPAAVRARVRTAYYGVPTSAIAVEQTHNRGGGAVWPLATLEELRA"
     gene            581..1819
                     /locus_tag="VF995_02650"
     CDS             581..1819
                     /locus_tag="VF995_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEX9342503.1"
                     /translation="MSTTCTETLEITGHVIDSMLFTRILDQVRSVMADYVIEQFDVGH
                     TPADPSYARLLIKADDPAQLEELVQSLMGLGMTRVDVEAGAADLVPAETDGVLPDGFY
                     SSTNLETEVHDDGRWLRVENPEMDCAIVVKDRRARTVPMSDVLKGDPVVVSSQGIRVR
                     PRDRGRQGEVFEFMSSQVSSEKPQGLLVRRVAAAMRATRQRGERILWVAGPAVVHTGA
                     GPAVTRLIEGGYMDVLFAGNALATHDIEGALYGTSLGVNLAEGIGVEHGHEHHIRALN
                     TVRRCGSIAKAVEQGVLTTGIMHACVLHGVDYVLGGSVRDDGPLPDTVTDVIDAQRQM
                     RTLLPGVGYAIMVATMLHSIATGNLLPAAVPLVCVDINPATVTKLADRGSTQATGVVT
                     DVGLFLGQLADELAPAPRRP"
     gene            complement(1715..2383)
                     /locus_tag="VF995_02655"
     CDS             complement(1715..2383)
                     /locus_tag="VF995_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX9342504.1"
                     /translation="MSGGLLWYVARAGGLVAWALAASAVIWGLALSTRVLGRRPRPAW
                     LFDLHRFLGGLALVFTGVHVVAVLGDSYVSFSLVNVLVPLTGSWHPVAVAWGIVGFYL
                     LLAVELTSLARRHVPLRIWRRVHYGSFILFASTTVHALTAGTDRHAAAFLVAVVAVCG
                     LVALLTVVRLAMARAAPPAPAPARARAASGAPRGRGQLVGELAKEQADVGDHAGRLRR
                     APVG"
     gene            complement(2433..3473)
                     /locus_tag="VF995_02660"
     CDS             complement(2433..3473)
                     /locus_tag="VF995_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX9342505.1"
                     /translation="MEGEARFRAMGSDVHVIVVGGRVTLLEAARELIEELELRWSRFK
                     PSSEISRLNAHRAEPVMVSPPTLALVQRALEGAHITGGRYDPTVLDAVLQAGYDRSFE
                     LLANPATATATGAGPAPGPDGGGRLPGAPPRHRHRAWRAIAVDPEASTVTLPGGVGFD
                     PGGIGKGYAADLLVAELRRQGAAGVCANLGGDLRVEGEPSAGRSWRVAIEHPLWRTPV
                     ATVGLRSGAVATSSRTRRAWGPPHDRRHHLIDPATGLPARSGVATASVVAAEAWQAEV
                     LAKALFIGGLGDGFALLDQTGTEGLLIDDHGAMHPSAEFGRFIGATGRRPSVAAGVAE
                     PPFEFEFEEALR"
     gene            3526..3819
                     /locus_tag="VF995_02665"
     CDS             3526..3819
                     /locus_tag="VF995_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342506.1"
                     /translation="MSGLADQEVEPELAVLPELDDEDEEDEDEVVVLVAVVVVSAADL
                     AAIPAPSPRNRTALSAPATIRERKAAWRRRRRRAGGFGGRGARSSMANLRLVG"
     gene            4046..4264
                     /locus_tag="VF995_02670"
     CDS             4046..4264
                     /locus_tag="VF995_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342507.1"
                     /translation="MIDPASMGDWAQATALGVSFGGLALAGVLAFRKVRHEELQEDLG
                     AELIAIEARADQYRAERARMAEMGTRGA"
     gene            4264..5007
                     /locus_tag="VF995_02675"
     CDS             4264..5007
                     /locus_tag="VF995_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9342508.1"
                     /translation="MEASAALGGREQPASRLLVVEDDPNILELLSASLRFAGFEVVTA
                     TGGFEAIRAVERHRPDLVVLDVMLPDVDGFDVARRLRSGNATTSAGSALPVLFLTARD
                     ATEDKVAGLTLGGDDYVTKPFSLEEVIARIRTILRRAGSGPPPVPTRLRVADLELDEE
                     SHEVWRAGQRIPLSPTEFKLLRYLMSNAGRVLSKGQILDYVWNYDFQGDARIVESYVS
                     YLRRKIDFVEPRLLHTIRSVGYVLRVADE"
     gene            5043..6638
                     /locus_tag="VF995_02680"
     CDS             5043..6638
                     /locus_tag="VF995_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018744082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9342509.1"
                     /translation="MTGARWGGIRRLASRPWSASQRLLARTPLRVKLIVAVLLLVTVA
                     LGLISVASIASLRGYLVSRLDNQLQLVASRPAPNPLQNGGGSGNGGGGEGQNNQRSQP
                     PSPYWVQFLDADGNVRRDSSGNVMVFSNPLLQGQQPPKLRYDAAWLRAHAGGPFTLAA
                     TSGASHWRVLVQFHLDGTSTVAAASLDDISGTITRLQRIDLIVSAVVLVVLAGIVVMI
                     VRANLRPLVEIEQVAGAIAEGELTRRVPDRDPRTEVGQLGRALNAMLAQIEAAFGARA
                     ASEVAARRSEERMRRFVSDASHELRTPLTTIRGFAELYRQGAAREPAELDRLMRRIED
                     QAASMGLLVNDLLLLARLDQQRPLERHPVDLLTLVADAVQDARAVAPDRQVALLLGDG
                     DAPGEPLIVLGDESRLRQVIANLMSNALTHTPPSTSVEVRARTDAQHAVVEVADHGPG
                     LTPEQTERVFERFYRADPARSHAQAGTSGTDGGAGAGLGLAIVAALVAAHSGTVEVDS
                     TPGRGATFKLLLPLAPDTAEAPA"
     gene            complement(6635..6970)
                     /locus_tag="VF995_02685"
     CDS             complement(6635..6970)
                     /locus_tag="VF995_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342510.1"
                     /translation="MTDASAIRASSDDYQAFLESQETTTLRDWALRVARERHDVGFFW
                     DLAKHLPDVEDANLDWAAYDPIDAIRELTHLVTHFREEAAGPDVASLLRIRYVEYLVD
                     AGEHRFDKA"
     gene            complement(7031..9346)
                     /locus_tag="VF995_02690"
     CDS             complement(7031..9346)
                     /locus_tag="VF995_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9342511.1"
                     /translation="MSQPATLPATVRTSLTRPAWLLVVASMAVAAIVAGSLAPSAVHD
                     LTHGQTGVVWTALLYAGGLVMAERFVVKVPVRNHRFSIGVTDMVIALGFVFLAPPLLV
                     LATAVGIAISQWLFESQLVKRLFNVPQYMLSVAAAASVALAMRTLLHHLRPFGIDVPA
                     TATGSFKPSLAVVWVVGMAAFFLVNHSLVSIVISLLVGQGFVQCWLRAAPVALADWAA
                     STAYGLVIAALLGHDRALLPFLIVPIVLTFIGNRAWARSLAQGQRMHSLYAAGRALSN
                     RLGDISAWQEFVQQVPEVLNCEGAAVFLGRADDGTLEVVESGGGFDRLNVSPSPVAWE
                     QAAKGYATRADWRRSTMVMMEVEGRVVGYAVAFGPRVDADFTQDDRDILLTLANQGAA
                     ALLNEELFREAENERTTLRDIVGHSTDGIYTVGPDRTVRSWNPAMVALTGYAEDEAVG
                     QKCFNLLRARDDNGVDMCAADCPILVAARSGHEEVRDASVLNKDGVARWIRYNHAPVM
                     GSDGDMDADVVVVRDVTRERQTDELKADFVASVSHELRTPLTPIKGFLMTLLREDRDF
                     TQERRREYYKLMLTQTQRLERLIEDLLEVTRLEAGADLVDAIAIDAIDLVRQVVDRST
                     AEDPERIINVRTPGHAVYCRGDWMRTDQVLGNLLSNAMRYSPPHETVEVRLVPQGREV
                     VFEVRDAGPGIPNDEQSRIFERFHRVGHYLTREPGGAGLGLYLAKRLVEAMGGRIWVS
                     SRLGHGSVFSFALPSEPSLNAVRLDDRRRLG"
     gene            complement(9432..9743)
                     /locus_tag="VF995_02695"
     CDS             complement(9432..9743)
                     /locus_tag="VF995_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342512.1"
                     /translation="MRTHNLHACHLTPLRPASPEQVRKGEEFHACRTVPSYSQDRNHA
                     GFSRFEHLVPSGCLLDMVASNGLVDVVARVLDVVASGRLQYLVASGRVVDVVLSWGFL
                     D"
     gene            complement(9959..>10360)
                     /locus_tag="VF995_02700"
     CDS             complement(9959..>10360)
                     /locus_tag="VF995_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9342513.1"
                     /translation="ALAGRVADAWNAWGVTPDELAAGLATVRQAAEQAGRDPATVRAT
                     WGGQVLIGTDAAEARVLLDRWAERREPQDVARTVAGDAATVLARLHELGEAGAVWCIA
                     ALVGGPDQQLASMRSLLAKAAGFAMRVPASA"
BASE COUNT         1558 a         3632 c         3739 g         1431 t
ORIGIN      
        1 gcgcgcagtt cctccagggt ggcgagcggc cagaccgcgc ccccgccgcg gttgtgggtc
       61 tgctccaccg cgatggcgct ggtcggcacg ccgtagtagg cggtgcgcac gcgggcgcgg
      121 accgccgccg ggtcgagcag cccgcgcgga ccggtcatgg cgtggaactg gatgccgctg
      181 agcagcgacc cggccgcgtt ctcgtaccag aagatgtggt tgtcctcatg cgccaggcac
      241 tcgctgcccg gcgcggcgcc gagccgcaga cagatctggt tgcccatgtg cccagacggc
      301 acgaacaggg cggcctcgcg gccgaaccgc tcggcgaccg ccgcctccag ccgcttggtg
      361 gtgggatcct cgcgccactg gtcgtcgccg acctcggcgt cggccatggc ctggcgcatc
      421 gccggggtcg gtttcgtcat cgtgtcggaa cgcaggtcgg tcgtcatcgc cgacgattgt
      481 agcgagggcc tgccgcgacg gccctggtga cctgggcgtg gcacaattcg ccgggtcagg
      541 ctgcgcaaga ccgcgtcgca accgacctga tcggggggcc atgtccacga cctgtacgga
      601 aacgctggag atcaccgggc atgtgatcga ctcgatgctg ttcacccgca tcctcgacca
      661 ggtgcgcagc gtcatggccg actatgtgat cgagcagttc gacgtcggcc acacgcctgc
      721 cgacccgtcc tacgcccgcc tgctgatcaa ggccgacgac cccgcgcagc ttgaggaact
      781 ggtccagtcg ctgatgggcc tcggcatgac ccgtgtggac gtcgaggccg gcgccgccga
      841 cctggtcccc gccgagaccg acggcgtgct gcccgacggc ttctactcct ccaccaacct
      901 ggagaccgag gtccacgacg acggccgctg gctgcgggtc gagaaccccg agatggactg
      961 cgccatcgtg gtgaaggacc gccgtgcccg caccgtcccg atgagcgacg tcctcaaggg
     1021 cgacccggtg gtggtctcca gccagggcat ccgcgtgcgc ccccgcgacc gtggccgcca
     1081 gggggaggtc ttcgagttca tgtcctccca ggtctccagc gagaagccgc agggcctgct
     1141 cgtccgcagg gtcgccgccg cgatgcgcgc caccaggcag cgcggcgagc ggatcctgtg
     1201 ggtggctggc cccgccgtgg tccacaccgg cgcggggccg gcggtgacgc ggctgatcga
     1261 ggggggctac atggacgtgc tgttcgccgg caacgcgctg gccacccacg acatcgaggg
     1321 cgccctctac gggacctcgc tgggggtgaa cctcgccgag gggatcggcg tcgagcacgg
     1381 ccacgagcac cacatccgcg ccctcaacac ggtccgccgc tgcgggtcga tcgccaaggc
     1441 ggtcgagcag ggggtgctca ccaccgggat catgcacgcc tgcgtgctcc acggcgtcga
     1501 ctacgtgctc ggcgggtcgg tccgcgacga cgggccgctg cccgacaccg tcaccgacgt
     1561 gatcgacgcg cagcggcaga tgcgcacgct gctgcccggg gtcggctacg cgatcatggt
     1621 ggcgaccatg ctgcattcga tcgccaccgg gaacctgctg ccggccgcgg tgccgctggt
     1681 ctgcgtcgac atcaacccgg cgacggtgac caagctagcc gaccggggct cgacgcaggc
     1741 gaccggcgtg gtcaccgacg tcggcctgtt ccttggccag ctcgccgacg agctggcccc
     1801 ggcccctcgg cgcccctgac gctgcccgtg cccgtgccgg ggccggtgcc ggaggggctg
     1861 cgcgggccat cgccaggcgg accacggtca gcagcgccac cagcccgcag accgcgacca
     1921 cggcgaccag gaaggcggcg gcgtggcggt cggtgccggc ggtcagggca tggacggtgg
     1981 tggaggcgaa caggatgaag ctgccgtagt ggacccggcg ccagatccgg agcgggacgt
     2041 ggcggcgcgc cagcgaggtc aactccaccg cgagcaggag atagaagccg acgatgcccc
     2101 acgcgaccgc gacggggtgc cagctgccgg tcagggggac caggacgttg accaggctga
     2161 agctgacgta gctgtcaccg aggaccgcga ccacgtggac gccggtgaac accagcgcca
     2221 ggccgccgag gaagcggtgc aggtcgaaca gccaggccgg gcgcggcctg cggccgagca
     2281 cccgggtcga gagcgcgagg ccccagatga ccgcgctggc ggccagcgcc caggcgacca
     2341 ggccgcccgc ccgggccacg taccagagca ggccgccgct cattcggcac gctccggcat
     2401 cgtcgcccac cgcagcgcgc tcaccgtgga actcaccgga gtgcctcctc gaactcgaac
     2461 tcgaacgggg gctcggccac gcccgcagcg accgagggtc ggcgcccggt ggcgccgatg
     2521 aaacggccga actcggcgct cgggtgcatc gccccgtggt catcgatcag caggccttcg
     2581 gttccagtct ggtcgagcag ggcgaacccg tcgccgaggc cgccgatgaa cagggccttg
     2641 gccaggacct cggcctgcca ggcctcggcg gcgaccacgc tggcggtggc gacgccgctg
     2701 cgggccggga gtccagtcgc cgggtcgatc aggtggtggc ggcggtcatg tggcggaccc
     2761 caggcgcggc gggtccggct gctggtggcg acggcgccgc tgcgcagccc gacagtcgcc
     2821 accggcgtgc gccacagggg gtgctcgatc gcgacccgcc aggagcgccc cgccgacggc
     2881 tcgccttcga cccgcaagtc gccgccgagg ttcgcgcaca ccccggcggc gccctggcgg
     2941 cgcagctcgg cgaccagcag gtcggcggcg tagcccttgc cgatcccgcc cgggtcgaag
     3001 ccgacgccgc cgggcagggt gacggtggag gcctccgggt cgaccgcgat cgcccgccag
     3061 gcgcggtggc ggtggcgcgg cggggccccg gggagccgcc cgccgccgtc cgggccggga
     3121 gcggggccgg cgccggtggc ggtggcggtg gcggggttgg ccagcagctc gaagctgcgg
     3181 tcgtagccgg cctggagcac cgcgtcgagc accgtcgggt cgtagcggcc gccggtgatg
     3241 tgggcgccct ccagggcccg ctggaccagc gcgagggtcg gtggggagac catgaccggc
     3301 tcggcgcggt gggcgttgag ccggctgatc tcgctgctcg gcttgaagcg gctccagcgc
     3361 agctccagct cctcgatcag ctcgcgggcg gcctcaagca gggtcacccg cccgccgacg
     3421 acgatgacgt ggacgtcgct gcccatcgcc cggaaacgtg cctcgccttc catcgttgtg
     3481 cctcagcttc ctgagctggt ggtgatcgcc tgaccagagg atgcggtgtc agggctcgcc
     3541 gaccaggagg tagagcccga gctggccgtg ctgcccgaac tcgacgacga ggacgaggag
     3601 gacgaggacg aggtggtggt tctggttgcc gtggtggtgg tgtcggccgc tgacctggcc
     3661 gccattcccg cgccaagccc caggaacagg acggcgctga gcgcacccgc gaccatccgg
     3721 gagcggaagg cggcgtggcg ccggcggcgc cgccgggccg gggggttcgg ggggcgcgga
     3781 gcccggtctt ccatggcaaa cctccgattg gttggttgat tgcccgcttc gagcttgcac
     3841 gtcccggctg gggccgagct gcgaggtagc tgggagcttc ctgaacgtcc tgctggatac
     3901 cggaaacgcc cgattggcag ggctcgtggc caatgactcc cagcaaactc ccagctgagt
     3961 ttcaggttgt tcgtgggcgc ggcggttaga ccgaagacaa ggacaggatc cgacttgtcg
     4021 agcaccaacg agagaggcga acaagatgat tgacccggct tcaatgggcg attgggcgca
     4081 ggcgacggcg ctgggcgtgt cgtttggcgg cctggcgctc gcgggtgtgc tggcgttccg
     4141 caaggtgcgg catgaggagt tgcaggagga tctcggcgcg gagctgatcg ctatcgaggc
     4201 ccgcgcggac cagtaccggg ccgagcgtgc gaggatggcg gagatgggga cgagaggagc
     4261 gtgatggagg cgtcagccgc gctgggcggc cgggagcagc cggcgtcgag gctgctggtg
     4321 gtcgaggatg acccgaacat cctcgagctg ctgtcggcca gcctccggtt cgctggtttc
     4381 gaggtcgtca cggccacggg cgggttcgag gcgatccggg cggtggaacg ccaccgcccg
     4441 gatctcgtcg tgctcgacgt gatgctgccg gacgtggacg gcttcgacgt cgccaggcgg
     4501 ctgcgctccg gcaacgccac caccagcgcc ggcagcgcgc tcccggtgct gttcttgacc
     4561 gcgcgggacg ccaccgagga caaggtcgcc gggctgacac tcggcggcga cgactacgtc
     4621 accaaaccgt tcagcctgga agaggtcatc gcccgcatcc gcacgatcct gcgccgcgcc
     4681 ggcagcggcc cgccgccggt cccgacgcgc ctgcgcgtcg ccgacctgga actggacgag
     4741 gagagccacg aggtgtggcg cgccggccag cgcatccccc tgtcccccac cgagttcaag
     4801 ctgctccgct acctgatgag caacgcgggc cgggtcctgt ccaaggggca gatcctcgac
     4861 tacgtctgga actacgattt ccagggcgac gcccgcatcg tcgaatccta cgtgtcgtac
     4921 ctgcgtcgca agatcgactt cgtggaaccg cgcctgctgc atacgatccg cagcgtcggc
     4981 tacgtcctgc gggtcgcgga cgagtagcga ccctcgatcc gacaaatcca gaggcgtcag
     5041 tcatgaccgg tgcgcgatgg ggtgggatac ggcggctggc cagccggccg tggtcggcga
     5101 gccagcggct cctggcgcgc actcccctgc gcgtcaagct gatcgtcgcc gtgctgctgc
     5161 tggtgaccgt cgcgctcggc ctgatcagcg tggcgagcat cgcctccctc cgcggctacc
     5221 tggtcagccg cctggacaac cagctgcagc tggtggccag ccgtccagcc cccaacccgc
     5281 tgcagaacgg tggcggaagc ggaaacggtg gcgggggtga gggccagaac aatcagcggt
     5341 cccagccgcc cagcccgtac tgggtgcagt tcctcgacgc cgacggcaac gtcaggcgcg
     5401 actcgagcgg caacgtcatg gttttcagca accccctgct gcagggccag cagccaccca
     5461 agctgcgcta tgacgccgcc tggctgcggg cgcacgccgg ggggccgttc accctcgcgg
     5521 ccaccagcgg cgcaagccac tggcgcgtgc tcgtccagtt ccacttggac gggaccagca
     5581 ccgtcgccgc cgccagcctc gacgacatca gcggcaccat cacacgcctg cagcgcatcg
     5641 acctgatcgt cagcgccgtg gtgctggtgg ttctggccgg catcgtggtg atgatcgtgc
     5701 gggccaacct gcggccactg gtcgagatcg agcaggtcgc gggcgcgatc gccgaaggcg
     5761 agctgacccg gcgggtgccc gaccgggacc cgcgcaccga ggtggggcag ctcggccggg
     5821 cgctcaacgc catgctggcg cagatcgagg ctgccttcgg cgcccgcgcc gcctccgagg
     5881 tcgccgcgcg ccgctccgag gagcggatgc ggcgcttcgt ctccgacgcc agccacgagc
     5941 tgcgcacccc cctgacgacc atccgcggct tcgccgagct gtaccggcag ggcgcggcgc
     6001 gggagccggc cgagctggac cggctgatgc gccgcatcga ggaccaggcc gccagcatgg
     6061 gcctgctggt caacgacctg ctgctgctcg cccgcctgga ccagcagcgc cccctggaac
     6121 gccacccggt ggacctgctc accctggtcg ccgacgcggt ccaggacgcc cgcgcggtcg
     6181 ccccggaccg ccaggtcgcg ctgctgctcg gcgacggcga cgctcccggc gagccgctga
     6241 tcgtgctcgg cgacgagtcg cgcctgcgcc aggtgatcgc gaatctcatg agcaacgcgc
     6301 tcacccacac ccccccgagc acctcggtcg aggtgcgggc acgcaccgat gcccagcacg
     6361 ccgtcgtgga ggtcgccgac cacggccccg gcctcacccc cgagcagacc gagcgcgtct
     6421 tcgagcgctt ctaccgcgcc gacccagccc gctcccacgc ccaggccggg acctccggaa
     6481 ccgacggcgg cgccggcgcc gggctcggcc tcgcgatcgt cgccgctctg gtcgccgccc
     6541 acagcggcac cgtcgaagtg gacagcaccc caggcagggg cgcgaccttc aagctgctgc
     6601 tgccgctcgc ccccgacact gccgaggccc cagcctaagc cttgtcgaaa cggtgctcgc
     6661 cggcgtcgac caggtactcg acgtagcgga tccgcagcag ggaggccacg tccggccccg
     6721 ccgcctcctc gcggaagtgc gtcaccagat gggtcagctc acggatcgcg tcgatcgggt
     6781 cataggcggc ccagtccagg ttggcgtcct cgacgtccgg caggtgcttg gcgagatccc
     6841 agaagaaccc gacgtcgtgc cgctcacggg cgacgcgcag cgcccagtcg cgcagcgtcg
     6901 tcgtctcctg cgactccagg aacgcctggt agtcatcgct gctggcacgg atggcactgg
     6961 catcggtcat cgtcgggctt cctccgctcg gaaccgggct tcctcggcgg aggccacctt
     7021 accgcctgtg tcacccgagg cggcgacggt cgtccaggcg gacggcgttg aggctcggct
     7081 cggaggggag ggcgaaggag aagactgagc cgtggccgag gcggctggag acccagatgc
     7141 ggccgcccat cgcctcgacc aggcgtttgg ccaggtagag gccgaggccg gcgccgcctg
     7201 gctcgcgggt gaggtagtgg ccgacacggt ggaaccgctc gaagatgcgt gactgctcgt
     7261 cgttggggat gcccggtccg gcgtcgcgga cctcgaagac gacctcgcgg ccctgcggca
     7321 ccagacggac ctcgacggtc tcgtgcggcg gggagtagcg catggcgttg gacaggaggt
     7381 tgccgagcac ctggtcggtc cgcatccagt cgccccggca gtagaccgcg tggccggggg
     7441 tgcgcacatt gatgatccgc tcggggtcct cggcggtgga gcggtcgacc acctggcgga
     7501 ccaggtcgat ggcgtcgatc gcgatcgcgt cgaccaggtc ggcgccggcc tccaggcggg
     7561 tcacctccag caggtcctcg atcaggcgct ccagacgctg ggtctgggtg agcatcagct
     7621 tgtagtactc ccggcgccgc tcctgggtga agtcgcggtc ctcgcgcagc agcgtcatca
     7681 agaacccctt gatgggggtc agcggggtgc gcagctcgtg cgagacgctg gcgacgaagt
     7741 cggccttcag ctcgtcggtc tggcgctcgc gggtgacgtc gcggaccacc accacgtcgg
     7801 cgtccatgtc gccgtcggag cccatcaccg gcgcgtggtt gtagcggatc cagcgggcga
     7861 cgccgtcctt gttgagcacc gaggcgtcgc ggacctcctc gtggccggat ctggcggcga
     7921 ccaggatcgg gcagtcggcg gcgcacatgt cgacgccgtt gtcgtcgcgg gcgcgcagca
     7981 ggttgaagca cttctgcccc accgcctcgt cctcggcgta gccggtgagg gccaccatcg
     8041 cgggattcca ggagcgcacc gtgcggtcgg ggccgacggt gtagatgccg tcggtggagt
     8101 ggccgacgat gtcgcgcagg gtggtgcgct cgttctcggc ctcgcggaac agctcctcgt
     8161 tgagcagcgc ggcggcgccc tggttggcca gcgtcagcag gatgtcgcgg tcgtcctggg
     8221 tgaagtcggc gtcgaccctg ggcccgaagg cgacggcgta gccgaccacg cggccctcga
     8281 cctccatcat caccatggtg ctgcggcgcc agtcggcgcg ggtggcatac cccttggcgg
     8341 cctgctccca ggcgaccggc gacgggctga cgttgaggcg gtcgaagccg ccgccgctct
     8401 cgacgacctc cagggtgccg tcgtcggcgc ggccgaggaa caccgctgca ccctcgcagt
     8461 tgagcacctc ggggacctgc tggacgaact cctgccaggc ggagatgtcg ccgagccggt
     8521 tggagagcgc gcggccggcc gcgtacaggg agtgcatgcg ctggccctgc gcgaggctgc
     8581 gcgcccaggc gcggttgccg atgaaggtga gcacgatcgg cacgatcagg aacggcagca
     8641 gcgcccggtc gtggccgagc agtgcggcga tcaccaggcc gtaggcggtg ctggcggccc
     8701 agtcggcgag cgcgaccgga gcggcgcgca gccagcactg cacgaagccc tgccccacca
     8761 gcaggctgat cacgatgctg accagcgagt ggttgaccag gaagaaggcg gccatgccga
     8821 ccacccagac gactgccagg ctgggcttga agctgccggt cgcggtcgcg gggacgtcga
     8881 tgccgaaggg gcgcaggtgg tgcagcagcg tccgcatcgc gagcgcgacg ctggccgccg
     8941 ccgccaccga gagcatgtac tgcgggacgt tgaacagccg cttgaccagc tgggactcga
     9001 acagccactg gctgatcgcg atgccgaccg cggtcgcgag caccagcagc ggcggtgcca
     9061 ggaacacgaa cccgagcgcg atcaccatgt cggtcacgcc gatcgagaac cggtggttgc
     9121 gcaccggcac cttgacgacg aaccgctcgg ccatgaccaa cccgccggca tacagcagcg
     9181 cggtccacac cacgccggtc tgcccgtgcg tcaggtcatg gaccgcgctg ggcgcgaggc
     9241 tgccagcgac gatcgcggcc acggccatac tggcgacgac cagcagccat gcgggccgcg
     9301 tcagcgaggt ccggacggtc gccggcaggg tcgccggttg gctcacgaca ccatcgaggt
     9361 tgggtccctt tcacgcatgc ggcaatccgc accttgagcg tgaccagaga gaagagacta
     9421 acggtcacac ctcagtctag gaagccccaa ctcaggacca cgtcgacgac gcgaccggac
     9481 gcgaccaggt actggaggcg accggacgcg accacgtcga ggacgcgagc gaccacgtcg
     9541 acgaggccgt tggacgcgac catgtcgagg aggcaaccgg acgggaccag gtgctcgaag
     9601 cgtgaaaaac ctgcatgatt ccggtcttgg gaataggacg gcacagtgcg gcaggcatgg
     9661 aactcctccc ctttgcgtac ctgctcgggg gaagcaggcc gcagcggcgt taagtggcag
     9721 gcatgaagat tgtgcgtgcg cacggtcaga agcgcaatgc cgtttcccca acaaacggta
     9781 ctaataatcg caacgatgcc tagactttat ttgctagtca atcagccctg gctcgccctg
     9841 ctctggccgt gagcagcgag aacctagcag gtttcggcga agctgtcttg tcaggcgctc
     9901 gccttgccaa tatcgcctgg ctggacggat ccgtccggcg agcagattct ccgctgaatt
     9961 acgcactcgc tgggacgcgc atggcgaatc cagcggcttt ggcaagcaat gaccgcattg
    10021 aagcgagctg ctggtcgggt cctccaacca gtgcggcaat acaccacacc gcgcccgctt
    10081 cgcccagctc gtgcaggcgg gccaaaaccg tggcggcgtc accggcaacg gtccgcgcca
    10141 catcctgggg ctcgcgccgc tcggcccagc ggtccagcag cacccgggcc tccgccgcgt
    10201 cggtaccgat caggacctgc ccgccccagg tggcacgcac cgtcgcgggg tcgcgcccgg
    10261 cctgctcggc tgcctggcgt accgtcgcca agccggcggc gagctcgtcg ggggtcaccc
    10321 cccaggcgtt ccaggcgtcc gccaccctgc cggcgagcgc
//
LOCUS       DASWDC010000083         9517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_685658, whole genome shotgun sequence.
ACCESSION   DASWDC010000083 DASWDC010000000
VERSION     DASWDC010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9517
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685658"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VF995_02705"
     CDS             complement(<1..338)
                     /locus_tag="VF995_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342514.1"
                     /translation="MLPTTDPRLFDLQLEDPARPRAGARAGNGASRRRRRPAPLLLAV
                     DGNGLTHRAFHAVGGADAPPGAAVERVVAMLARLAAELRPTACVIGFDDAGRSLRRER
                     CAHYKSARPP"
     gene            464..1837
                     /locus_tag="VF995_02710"
     CDS             464..1837
                     /locus_tag="VF995_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007383860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342515.1"
                     /translation="MKLRLRPLLLAVLVVAAMLGGATAAHADGPTTLTGTLPDGATWK
                     AEVPSPWNGTLILYNHGYVAPLPGAANPAVDVSDPLTGGYLLGQGYALAGSSYASTGW
                     AVKDALSDQLAVLDVFAQQVGKPAATYTWGDSLGGMITAGLVQTAPSRFAGALPMCGV
                     LSGGVGTWNQGLDSAFALKTLVAPNSALQVVHITDPFGNLNLAEQGLAAAQTTPQGRA
                     RIALAAAVADVPGWFNPASPEPAPGDVATQEANQFTAFQQQGIPFAFAFRAELEARAG
                     GNPSWNTGVDYAAQLRKSVDRKEVAALYQQAGLSLSADLATLNQAPRISADTPAVRYL
                     TQFITYNGRLGHIPVLTIHTTGDTLVVVENEQAYASVVARDGNTGQLREAFVHRANHC
                     TMTPAERIAALQALFDRVHSGHWDTSPASLDARAAALPSSLQLAPPSYVTFRPAPFLR
                     PFDRLSH"
     gene            complement(2024..3190)
                     /locus_tag="VF995_02715"
     CDS             complement(2024..3190)
                     /locus_tag="VF995_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018813479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX9342516.1"
                     /translation="MTTRKYALPVEQTRWHVPSGSTTTFSWDYDEGRDRLLTLYEKGK
                     RRQWDAAVRIDWSQEIGLDNPLRIPDESVPIYGSRTWDRLGERDRAIVRRHSASWQFS
                     QFLHGEQGALICASKIVQTVPDLDAKFYAATQVMDEARHVEAYSRYLHDKLQLAYPIN
                     PNLKALLNDVIADSRWDMTYLGMQVLIEGLALAAFGLIRDLTPEPLAKSVNAYVMQDE
                     ARHVAFGRLALREYYPQLTQAERDEREEFCVEACYLMRDRFLAEEVWRNLDYDPDDCI
                     EHVRTSETLRQFRSLLFSRIVPTLRDIGMFGPRIRAAFADMGVLGFEAVNVEELMAGD
                     EALAEQIDAARIQQVGETIEAGRAATEAATGAATGAATGAATGTGTGVGQAGTE"
     gene            complement(3333..3554)
                     /locus_tag="VF995_02720"
     CDS             complement(3333..3554)
                     /locus_tag="VF995_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342517.1"
                     /translation="MNPTAALLLLLVLLALAIPLFIRVARFEASCLADLAEAEGAELL
                     VFSRQGWAAIILLMIPIGGFLYLRFGRIR"
     gene            3642..3758
                     /locus_tag="VF995_02725"
                     /pseudo
     CDS             3642..3758
                     /locus_tag="VF995_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936596.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3898..5235
                     /locus_tag="VF995_02730"
     CDS             3898..5235
                     /locus_tag="VF995_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9342518.1"
                     /translation="MTTAPLRTGTAVLELLRAGRFAEIRDLFAPQLRPMVPVESLQAG
                     WTAALDQYGPVASVGAPLSEPAGPGAVVVKLPVRFERGEQTVAISVTDAGVLTGLQLL
                     PASAAQPTEPWEPPAYADPEQFDEQAVTVGDGPLAVPGTLSLPRLPREPRQPGAAPGP
                     WPAVVLLTGSGPHDRDETIGRNKPFKDLAWGLASRGVAVLRFDKVTYTHPSQLATARD
                     FTVADEYLPHAVAAVHLFQRHPAIDAARVFVLGHSLGGTVAPRVAAAEPRVAGLVVLA
                     GGTQPLHWAAVRQVRHLASLDSAMAAASEATIEALTRQARMIDSPGLSPSTPPSELPF
                     GVPASYWLDLRGYDPVATAAALGKPMLLLQGGRDYQVTVADDLAGWKAGLAGRSGVTI
                     RVYDADNHLFFPGTGPSSPAEYEPAQHLDPAVVADVASWLASTLPAPGADGLG"
     gene            5275..6216
                     /locus_tag="VF995_02735"
     CDS             5275..6216
                     /locus_tag="VF995_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9342519.1"
                     /translation="MRAVTIEDGRLAIREHADPVPSGGELLVRVRAAGLNGADLWQVR
                     GHYPAPPGSPQDIPGLELAGEVAAVGPGVTRFRQGDQVMGLVGGGGQAELAIVHERVA
                     MPVPDGVAWGAAGGFPEAFTTAHDALFTQCGLGLGDRVLVHGAAGGVGTAAAQLAAAA
                     GARVVASVRNPDHHRAVGFLGADQVVLPDAVEATGPFDIILELVGAPNFPTNLELLAT
                     GGRIMVIGTGAGPAIELDLRLLLARRARLLGSTLRSRPLEQKAEAARRVEAQVLPGLA
                     SGQLRVPVAATYPLKAAQTAYDHFAAGGKLGKIVLEP"
     gene            6352..7101
                     /locus_tag="VF995_02740"
     CDS             6352..7101
                     /locus_tag="VF995_02740"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX9342520.1"
                     /translation="MGISIFAATHRGLVRDGNEDSCFVGVTVIAVADGLGGHQAGEIA
                     SATAIEPVAKLDGQPFPTPEAARDAVVAAIEQGNQAVIEKAVAHDEYWGMGTTLTAAI
                     VGDGWLQLGHVGDSRAYLLRDAILRQLTNDHTIVAEAVRQGRLSPGEASHHPERSILT
                     RAVGLDPNLRVEAPPAFELEPGDQVLLCSDGLTEPVDEDQIALLLATTSDGQAACDAL
                     IDTANEAGGPDNITVILLRVDDADRASATVR"
     gene            7143..7805
                     /locus_tag="VF995_02745"
     CDS             7143..7805
                     /locus_tag="VF995_02745"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9342521.1"
                     /translation="MDRCRAVLFDWRGTLAHFPEPRWWVERALESLGRPPDLALVNSA
                     VTGLRAAAALPEVREAERHEDCSAASHRSVTMRRFELAGLDPDLAEALYRVDFDPASH
                     PLYPDASEVLAALRARGVRVALVSDIHFDLCAVLAEHGIAKFVDAYVLSFEHGFQKPD
                     RRMFTLALDALGAEPGEALMVGDRETHDGGAASVGIATLLLPMPPTLRPRGLEVVLRM
                     VG"
     gene            7946..8974
                     /locus_tag="VF995_02750"
     CDS             7946..8974
                     /locus_tag="VF995_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAF repeat-containing protein"
                     /protein_id="HEX9342522.1"
                     /translation="MRPTPSLLSRAAFLLLGTLLAASVLAAPASARPAARFRIIDLGT
                     LGGANSQALAVNNHGQVVGTSDVRPGSEDSHAFLWADGRMRDLGTFGGPISAALDIND
                     HGQVVGAADTASFVGHAFLWAHGRMRDLGTLGGTFSVATGINDQGVVSGYSATPSGAT
                     HAFLWRDGHMNDLGLGDSSTANDLNNHGQIVGGYPSPLGGFHAYRWQHGAATPIDTQS
                     NYSEAFAINARGQAAGWAGFPGSDFFHAALFSHGQTIDLGTLAGAFSKANGIDHDGRV
                     VGQSDSPTGPLHAFLWQGGRMTDLGALSDQGMNGGSSANDINDHGLIAGSSAVTTGDS
                     AHAVLWRG"
     gene            complement(9053..>9517)
                     /locus_tag="VF995_02755"
     CDS             complement(9053..>9517)
                     /locus_tag="VF995_02755"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742417.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX9342523.1"
                     /translation="VGLQYGVPLEALVAKFTNMIFEPNGLTDDPDVRIAQSLVDYVAR
                     RLAIDYLDTETRHALGIKTTAERIAEVNGTTPPASPNTNANTTTNTPAPASPPAQAPK
                     QAAPADNRGAGLTFTPGTMSVDAPFCSTCGVRMRPAGSCYVCESCGSTSGCS"
BASE COUNT         1340 a         3562 c         3278 g         1337 t
ORIGIN      
        1 ttgggcgggc gcgccgactt gtagtgggcg cagcgctcgc gccgcagcga gcgccccgcg
       61 tcgtcgaagc cgatcacgca cgcggtcggg cgcagctcgg cggcgagccg ggcgagcatc
      121 gctaccaccc gctcgaccgc ggcgccgggc ggcgcgtcgg caccgccgac cgcgtggaag
      181 gcacggtggg tgagcccgtt gccgtcgacc gccagcagca acggcgcagg ccggcgccgc
      241 cgcctgctgg ccccgttgcc ggcgcgggcg ccggcccgcg gccgcgctgg gtcctccagc
      301 tggaggtcga agagtcgcgg atcggtcgtc gggagcatgt ggacacgatc tccacttgtc
      361 gcggcgggtg caagcggcag ccaggcccgg accagctatt cccctgatcc ttactgaacg
      421 gtagcatccg cctgtcccaa tgatggagat gaggaggtct cggttgaagc tgcgccttcg
      481 tccgttgctg ctcgccgttc tggttgtcgc cgccatgctc ggcggcgcga cggcggcgca
      541 cgctgacggg cccaccacgc tcaccggcac gcttccggat ggcgcgacct ggaaggcgga
      601 ggtcccgtcc ccgtggaacg gcacgctgat cctctacaac cacggctatg tcgcgccgct
      661 gcccggcgcc gccaacccgg cggtcgacgt gagcgacccg ctcaccggcg gctacctgct
      721 cggccagggc tacgcgctgg ccggctcctc ctacgccagc accggctggg cggtcaagga
      781 cgccctctca gaccagctcg ccgtgctcga cgtgttcgcc cagcaggtcg gcaagcccgc
      841 cgccacctac acctggggcg actcgctcgg cggcatgatc accgccgggc tggtgcagac
      901 ggcaccctcg cgcttcgccg gcgcactgcc gatgtgcggc gtgctgagcg gcggggtggg
      961 cacctggaac cagggcctcg acagcgcctt cgccctcaag acgctggtgg cgcccaactc
     1021 ggcgctacag gtggtccaca tcaccgaccc gttcggcaac ctcaacctcg ccgagcaggg
     1081 cctggcggcc gcgcagacca ccccgcaggg ccgcgcccgc atcgcgctgg cggccgcggt
     1141 cgccgacgtc cccggctggt tcaacccggc ctccccggag cccgcgccgg gcgacgtggc
     1201 cacccaggag gccaaccagt tcaccgcctt ccagcagcag ggcatcccgt tcgcgttcgc
     1261 ctttcgcgcc gagcttgagg cccgcgccgg cggcaacccg tcgtggaaca ccggcgtcga
     1321 ctacgccgcg cagctgcgca agtccgtcga ccgcaaggag gtcgcggccc tctaccagca
     1381 ggccggcctg agcctgtcgg cggacctcgc caccctcaac caggcgccgc ggatctcggc
     1441 cgacaccccg gcggtgcgct acctcaccca gttcatcacc tacaacggcc gcctcggcca
     1501 catcccggtg ctcaccatcc acaccaccgg cgacaccctg gtcgtggtcg agaacgagca
     1561 ggcctacgcc agcgtggtgg cccgcgacgg caacaccggg cagctgcggg aggcgttcgt
     1621 gcatcgcgcc aaccactgca ccatgacccc ggccgagcgg atcgccgcgc tgcaggcact
     1681 gttcgaccgg gtccacagcg gccactggga caccagcccg gcctcgctcg acgcccgcgc
     1741 ggcggcgctg ccgtcgagcc tccagctggc gcccccgtcc tacgtcacgt tccggccggc
     1801 gccgttcctg cggccgttcg accggctgag ccactgagcc acagcgccgc ggcggcctga
     1861 cccgcaccaa gaaggcacgc gcccggcccc ggaattccgg ggccgggcgc gtcagcaccg
     1921 agccgtcccg ccggcgtccg cgctcgccgg cgaccggggg ggctaccgcc cggagccggt
     1981 ctggccgctc ggggggcgga cggccaacgc cggcggcggg cggctactcg gtgcctgcct
     2041 gtcccactcc tgttcctgtt cctgtcgccg ctcctgtcgc cgctcctgtc gccgctcctg
     2101 tcgccgcttc tgtcgccgct cttcccgcct cgatcgtctc ccccacctgc tggatccggg
     2161 ccgcgtcgat ctgctcggcg agcgcctcgt caccggccat cagttcctcc acgttgaccg
     2221 cctcgaagcc gagcacgccc atgtcggcga aggccgcccg gatcctcggg ccgaacatgc
     2281 cgatgtcgcg cagggtcggg acgatccggc tgaacagcag cgaccggaac tggcgcaggg
     2341 tctcgctggt cctcacgtgc tcgatgcagt cgtcggggtc gtagtcgagg ttgcgccaca
     2401 cctcctcggc gaggaagcgg tcgcgcatca ggtagcacgc ctccacgcag aactcctcgc
     2461 gctcgtcacg ctcggcctgg gtgagctgcg ggtagtactc gcgcagggcg aggcggccga
     2521 aggcgacgtg gcgggcctcg tcctgcatca cataggcgtt gaccgacttg gcgagcggct
     2581 ccggggtgag gtcgcggatc agcccgaacg ccgccagcgc cagaccctcg atcagcacct
     2641 gcatgccgag gtaggtcatg tcccagcggc tgtcggcgat gacgtcgttc agcagcgcct
     2701 tgaggttcgg gttgatcgga taggccagct gcagcttgtc gtgcaggtag cgggagtagg
     2761 cctcgacgtg gcgggcctcg tccatcacct gggtggcggc atagaacttg gcgtcgaggt
     2821 cgggcaccgt ctggacgatc ttggaggcgc agatcagcgc gccctgctca ccgtggagga
     2881 actgcgagaa ctgccaggac gcgctgtggc ggcggacgat ggcgcggtcg cgctcgccga
     2941 gccggtccca ggtgcgggaa ccgtagatcg gcaccgactc gtccgggatc cgcagcgggt
     3001 tgtcgaggcc gatctcctgc gaccagtcga tccggaccgc cgcgtcccac tggcggcgct
     3061 tccccttctc gtagagggtg aggagccggt cgcggccctc gtcgtagtcc caggagaacg
     3121 tggtggtgct gccgctgggg acatgccagc gggtctgctc gacgggaagg gcatactttc
     3181 gggtcgtcac aatctcggcc gcctcgccgc cctcggctcc ggttaccaga gggtaaatgg
     3241 cttgcggcat cgcgtcaaac gtatcgtctg ttgaagtttg tgacgttctg tcgatgcacc
     3301 gttcagccgc gactcagccg tgacgccagc gctcagcgga tcctcccgaa cctcaggtac
     3361 aggaacccac cgatcgggat catcagcaga atgatggccg cccagccctg gcgcgagaag
     3421 acgagcagct ccgcgccctc ggcctcggcg agatcggcca ggcaggaggc ctcaaaccgg
     3481 gccaccctga tgaacagggg aatcgcgagg gccaggagga ccaggaggag caggagcgcg
     3541 gcggtcgggt tcaacgaggt caatgaggtt ctccagctgc tggctaatct caccatcgag
     3601 aacgttagca agggtgagaa caaaccacta cggtgagcgg catggcaacc gctgatctcc
     3661 tcctgcaccc ggtccggcag cggatcctgc agaccttcct cggcgaccgg gcgctgacca
     3721 ccagcgaact gcgcgccgag ctggccgacg tccccgccgg ccccgggccg cgagcggcgc
     3781 atcatcggct acgtcctgct ccccggagac gatgcccctg gtgatggccg tggccggcac
     3841 gccgagcacc gccggccaga agggccataa ggccgcgaac caacggaggg atgaaggatg
     3901 acgaccgcgc cgctgcgcac tgggactgct gtcctcgaac tgctgcgggc gggacgcttc
     3961 gcggagatcc gcgacctgtt cgcgccgcag ctgcgcccga tggtgccggt cgagtcgctc
     4021 caggccggct ggacggccgc gctcgaccag tacggcccgg tcgcctctgt cggcgcgccg
     4081 ctgagcgagc cggccgggcc tggggcggtg gtggtgaagc ttcccgtgag gttcgagcgt
     4141 ggcgagcaga cggtggccat ctcggtcacc gatgcgggcg tgctgaccgg gctgcagctc
     4201 ctgcctgcca gcgcggcaca gcccaccgag ccgtgggagc cgccggccta cgcggatcca
     4261 gagcagttcg acgagcaggc ggtgacggtc ggcgacggtc ccctggcggt gcctggcacg
     4321 ctcagccttc cgcggctgcc gcgcgagccg cgccagccag gggctgcgcc ggggccgtgg
     4381 cccgctgtcg tgctgctcac cgggtcagga ccgcacgacc gcgacgagac gatcggacgc
     4441 aacaagccct tcaaggacct cgcctggggg ctggcgagcc gcggcgtggc cgtcctgcgc
     4501 ttcgacaagg tcacctacac ccacccgagc cagctcgcca ccgcgcgcga cttcaccgtg
     4561 gccgacgagt acctgccaca cgcggttgcg gccgtccacc tgttccagcg gcacccggcc
     4621 atcgacgccg cgcgggtctt cgtgctcggc cacagcctgg gcggcaccgt cgccccgcgc
     4681 gtcgcggcgg ccgagccgcg ggtcgccggg ctggtcgtcc tggccggcgg aacccagccg
     4741 ctgcactggg cggccgtccg gcaggtccgc cacctcgcct cgctggactc cgcgatggcc
     4801 gccgcatccg aggccaccat cgaggcgctc accaggcagg cgaggatgat cgacagcccg
     4861 ggcctctccc cctccacgcc gcccagcgag ctgcccttcg gcgtgccagc ctcctactgg
     4921 ctggacctgc gcggctacga cccggtcgcg accgccgcgg cgctcggcaa gccaatgctg
     4981 ctcctccagg gtggccggga ctaccaggtg acggtcgccg acgacctcgc gggctggaag
     5041 gccggcctcg ccggccgctc cggggtgacg atccgcgtct acgacgccga caaccacttg
     5101 ttcttccccg gcaccggccc gtcgtcaccc gcggagtacg agccggcgca gcacctggac
     5161 ccggcggtgg tcgccgacgt cgccagctgg ctggcgtcca ccctgccagc acccggggcc
     5221 gacggactag gctgaggccg ctccctctcc tctctccgac ctcaaggagc ggcaatgcgc
     5281 gcggtgacga tcgaggacgg ccgcctggcg atccgtgaac acgccgaccc cgtgcccagc
     5341 ggcggcgagc tgctcgtccg ggtgcgggcg gccgggctca acggcgccga cctgtggcag
     5401 gtccgcggcc actaccccgc cccgccgggc agcccccagg acatccccgg cctggagctg
     5461 gccggcgagg tggcggcggt tgggccgggc gtgacccgct tccggcaagg cgaccaggtg
     5521 atggggctgg tcggcggggg cggccaggcc gagctggcga tcgtgcacga gcgcgtcgcc
     5581 atgccggtcc ccgacggcgt cgcctggggg gccgccggcg ggttccccga ggcgttcacc
     5641 accgcccacg acgccctctt cacccagtgc ggcctcggcc tcggcgaccg ggtcctggtc
     5701 cacggcgccg ccggcggcgt cggcaccgcc gccgcccagc tcgccgccgc ggccggcgcc
     5761 cgggtggtgg cgagcgtccg caaccccgac caccacaggg ctgtcggctt cctcggcgcc
     5821 gaccaggtgg tgctgcccga cgcggtggag gccaccgggc cgttcgacat catcctggag
     5881 ctggtcggcg ccccgaactt ccccaccaac ctggagctcc tcgccaccgg cggccgcatc
     5941 atggtgattg gcaccggcgc cggtcccgcg atcgagctcg acctgcgcct gctgctcgcg
     6001 cggcgggcgc ggctgctcgg ctcgacgctg cgctcccgcc cactggagca gaaggccgag
     6061 gccgcccgcc gggtcgaggc ccaggtcctg cccggtctcg ccagcgggca gctgcgtgtc
     6121 ccggtcgccg ccacctatcc gctgaaggcg gcgcagaccg cctacgacca cttcgcggca
     6181 gggggcaagc taggcaaaat cgtgcttgag ccgtaggcga actgcggtcc ttgcaggggt
     6241 ccatgccgga tacgacacca gtcaactgga gaaagatgcg gccaggtgtg gccgggcggg
     6301 tgcgggagcg cgcagaatgc agctgctctc attggatgga ggacggcctt catgggcatc
     6361 agcatcttcg ccgcgacgca ccgtggcctg gtgcgggacg gcaacgagga cagctgcttc
     6421 gtgggcgtca cggtgatcgc ggtggccgac gggctcggcg gccaccaggc cggcgagatc
     6481 gcctcagcca ccgcgatcga gccggtcgcc aaactcgacg ggcagccctt cccgactccc
     6541 gaggccgcca gagacgcggt ggtggcagcg atcgagcagg gcaaccaggc ggtcatcgag
     6601 aaggccgtcg cccatgacga gtactggggc atgggcacaa cgctcacggc ggcgatcgtc
     6661 ggcgacggct ggctccagct cggccacgtc ggcgacagcc gcgcctacct cctccgcgac
     6721 gccatcctgc gccagctcac caacgaccac accatcgtcg ccgaagcggt ccgccagggc
     6781 cgtctcagcc ccggcgaagc cagccaccac cccgaacgca gcatcctcac ccgcgccgtc
     6841 ggcctcgacc ccaacctccg ggtggaggcc cccccggcct tcgagctgga gcccggcgac
     6901 caggtcctgc tctgctccga cggcctcacc gagccggtcg acgaagacca gatcgccctg
     6961 ctgctggcca ccaccagcga cggccaagcc gcctgcgacg ccctgatcga caccgccaac
     7021 gaggccggcg gccccgacaa catcaccgtg atcctgctcc gggtcgacga tgccgacagg
     7081 gccagcgcaa ccgtccggta ggaaccagga cgcccgggcg gcgcagctgg caaactctgg
     7141 ccgtggaccg ctgtcgcgcc gtgctcttcg actggcgagg aaccctcgcc cactttccgg
     7201 aaccgaggtg gtgggtcgaa cgcgcgctgg agtccctcgg gcggccgcct gacctcgcgt
     7261 tggtcaacag cgccgtcacc ggcctacgtg cggcggcggc gcttcccgag gtgcgggagg
     7321 ccgagcgcca cgaggactgc tcggccgcgt cgcaccgctc ggtcacgatg cgccgcttcg
     7381 agctcgccgg cctcgatccc gacctcgctg aggcgctcta ccgcgtcgac ttcgacccgg
     7441 cgagccatcc cctgtacccg gacgcctccg aggtgctcgc ggccctgcgg gctcgtggcg
     7501 tgcgggtcgc gctggtcagc gacatccact tcgacctgtg cgccgtgctc gccgagcacg
     7561 ggatcgcgaa gttcgtcgac gcctacgtgc tgtcgttcga gcacggcttc cagaaacccg
     7621 accgtcgcat gttcaccctt gccctcgacg ctctcggcgc cgagccgggt gaggcgctga
     7681 tggtcggcga ccgcgagacc cacgacggcg gcgccgcctc ggtcggcatc gccactctgc
     7741 tgctgccgat gccaccgacg ctgcggcctc gcggcctcga ggttgtcctc cgcatggtcg
     7801 ggtaggcgct cgggttggcg gatgagcgat cagatcgtta tcggcggatc gtgtagcgag
     7861 accggatcat gctgatttcg tgaccacgac ctgagacgtt ctcagcctgc agcacgcctg
     7921 tcgcgacccc tgggggaggg ctctcatgcg accaacacca tccttgctgt cccgtgcagc
     7981 attcctgctg cttggaacct tgctggcggc cagcgtcctg gccgcgccag catccgcccg
     8041 accggcggca cggttccgca tcatcgacct cggcaccctt ggcggggcga acagccaagc
     8101 tctcgccgtc aacaaccacg gccaggtcgt cggcaccagc gacgtccgtc ccggcagcga
     8161 agacagccac gcgttcctgt gggccgatgg ccgcatgcgc gacctcggca ccttcggcgg
     8221 ccccatcagc gcggcgctgg acatcaacga ccacggtcaa gtcgtgggcg ccgccgacac
     8281 ggcatcgttc gtcgggcatg cgttcctgtg ggcgcacggg cgcatgcgcg acctcggcac
     8341 gctcggcggc accttcagcg tggccaccgg catcaacgac cagggggtgg tcagcggcta
     8401 cagcgccacc ccgtccggtg cgacgcacgc gttcctgtgg cgcgacgggc acatgaacga
     8461 cctcgggctc ggcgattcca gcaccgccaa cgatctcaac aaccacggcc agatcgtcgg
     8521 cgggtacccg agcccgctgg gcgggttcca cgcctaccgc tggcagcacg gggccgcaac
     8581 gcccatcgac acgcagtcga actacagcga ggcgttcgcg atcaacgccc gcggccaggc
     8641 cgccggctgg gcaggcttcc caggttccga cttcttccac gccgcgctct tctcgcacgg
     8701 ccagacgatc gacctcggca ccctcgccgg tgcgttcagc aaggcgaacg gcatcgacca
     8761 cgacggccgg gtcgtcggcc agagcgactc gcccaccggc ccgctgcacg ccttcctgtg
     8821 gcagggcggc cgcatgaccg acctcggcgc cctctccgac cagggaatga acggcggcag
     8881 cagcgccaac gacatcaacg accacggcct catcgccggc tccagcgccg tcaccaccgg
     8941 cgacagcgcc cacgccgtcc tctggcgagg gtagcggcct ggcggtggtg ctcaacgcct
     9001 gagggggaag ctcgcagcct gcgggcttcc ccctccgccg cgcctgctgg tatcagctgc
     9061 agccgctggt cgagccgcag gactcgcaga cgtagcagga gccggcgggg cgcatcctga
     9121 ccccgcaggt cgagcagaac ggcgcgtcca ccgacatcgt gcccggcgtg aaggtgagcc
     9181 ccgccccccg gttgtccgcc ggggcggcct gcttgggtgc ctgggccggc ggcgaggccg
     9241 gggccggggt gttcgtagtc gtgttcgcgt tcgtgttcgg cgaggccggt ggtgtggtgc
     9301 cgttcacctc ggcgatccgt tcggcggtgg tcttgatgcc gagggcgtgg cgggtctcgg
     9361 tgtcgaggta gtcgatcgcc aggcggcgcg ccacgtagtc gaccaggctc tgggcgatgc
     9421 ggacgtccgg gtcgtcggtg aggccgttgg gctcgaagat catgttggtg aacttcgcca
     9481 ccagcgcctc cagcggcacg ccgtactgca ggccgac
//
LOCUS       DASWDC010000084         8898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_343355, whole genome shotgun sequence.
ACCESSION   DASWDC010000084 DASWDC010000000
VERSION     DASWDC010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343355"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..208)
                     /locus_tag="VF995_02760"
     tRNA            complement(133..208)
                     /locus_tag="VF995_02760"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:His,seq:gtg)
     gene            326..817
                     /locus_tag="VF995_02765"
     CDS             326..817
                     /locus_tag="VF995_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9342524.1"
                     /translation="MVNEIKTERLWLRQLPVEEARALLNGTAQPELPWMAGYPIEGSM
                     IAAEAYVRAIDGGQQVGPYGLYQIVVTEEGLVVGDIGFHGPPDPVRSVTIGYGLAAPA
                     RGHGYVTEALRALIAWAMAQPEVRRVEGDAVHANLASQRVMERAGMRYVGSDVQLRYY
                     RYP"
     gene            883..1680
                     /locus_tag="VF995_02770"
     CDS             883..1680
                     /locus_tag="VF995_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9342525.1"
                     /translation="MNQTPTRVFLVTGASSGIGEAAARRLVREPAAHVILLARRRERL
                     EALAAELTAAGGSASVIGIDLTDYDAPGMVAAVLERDHGRLDLLVNNAGVGGRGSFAE
                     SGFAEVQRTMRVNFGAPVRLTEALLPLLRRSAPSAIVNVASVAGRVGRPGTSAYSASK
                     FALIGWSESLQLEERPHGVHVGLVVPGFVSTEGFPQRELLAHRATRWLVSTTDKVADA
                     ITEVGLGRKAERYAPRPWAVVPFLKVLLPGLYRRAVGGGRFTAAAKR"
     gene            complement(1714..2694)
                     /locus_tag="VF995_02775"
     CDS             complement(1714..2694)
                     /locus_tag="VF995_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342526.1"
                     /translation="MSDHRRSDRDPGLLSRRDLLRSATLGAGALGVSALVSACGSKGG
                     GSSSTGTAPAGSLKALAAGATQLSLLGTADAQNPLPVGRSLFTFGLVTPDNQLISGGS
                     PQVWLAKDDTSKALGPFPATSFHMSAYGLTGDKSPATELTSFYGAEVEAPSAGNWLIV
                     AVADLNGTHVAGQGGAPVSEKVVGAIGSKALSEPTPVAASTAARAKICTRNPPCTMHS
                     ISLDKALRSGRPTVISFATPLLCQSRMCGPVVDEQLVAFHQVGAAKANFIHVEEFPTR
                     DPSKPAKAFSAWGFQTEPWVFVIDKGGVIRARYMGPVTAVQIRDALHPLL"
     gene            complement(2856..3539)
                     /locus_tag="VF995_02780"
     CDS             complement(2856..3539)
                     /locus_tag="VF995_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9342527.1"
                     /translation="MITLVLADDHQAFAEGLGMVLDAEDDIELVGVARDGGEALELIS
                     SLAPTVLLLDAHMPNTSLTQVLRDAKVKAPQTKVMVLSADTRRETITAATQAGADGFL
                     AKDTSGRQVASMIRRLVDGEGGFFTPAGAARQPRDPSVELRVRTLSARERQILTMLAN
                     GYSNRRIAEECFLSLNTVRTHVQNVLVKLGVHSKLEAVAFALEHQVVPAGPGGVPAPW
                     PDDNDNDGV"
     gene            3920..4666
                     /locus_tag="VF995_02785"
     CDS             3920..4666
                     /locus_tag="VF995_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9342528.1"
                     /translation="MARVFLADCQPLFNEALEALIARDGQNEVVGQASSAPEVTDAVV
                     HLKPDLVVLDADLALASRPSLVEAILDELPATRVILLAQDTDLELLLMAIRAGALGVV
                     GKKSGTQTVLRAVQAVLDGEGVVPRAMLPALFRRLLDLGDRAADTPLNRLSPREQEVL
                     ALLGRGWSNARIGKELFISPHTVRTHVQNVLQKLEMHSKLEAATFAMQHELAAKLPAP
                     GSGPGAPEQHQGDAPRPPARKPARKQSTGR"
     gene            4888..8109
                     /locus_tag="VF995_02790"
     CDS             4888..8109
                     /locus_tag="VF995_02790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9342529.1"
                     /translation="MDDRTVATAAAPATTATASAPPPARDAIRDGEGFPRSVLLLVAI
                     LAVLAGAAALTDRAPGSGPVATIAVIGLLAALTAAGSLHIDFRYHDEIEALELFESVL
                     LVAVYALSGRLVVVVTAVAAALSESLLRVRPLKAAFNVAQWSVAAAAGSIAFAALRSG
                     TALTPRNLTALLAAMVVVALVNHCSLVVVFGLVRRERLGRVLRGLAEVILPGWVLGGA
                     INLAFGVLFLATWIWWPFAAPLFLLPLGILHWAARVYAAIRIDRTRLTGMQEATHLLV
                     SSLYPSEAMPAFLAAVRRCFEAEAVELVLAGDPSGDEATLWRSADGDPGEVGRTTPES
                     FGLAVLRLGTAVRARAGGDGELAAPLALAGWRDCLAAPLRAGAAAGMLCTYNRNGLEG
                     FEEGELAVLEALAAELGTALEKSDLLQTMLEERAKLSEIVDHTSDGIAALDPDGTVSS
                     WNPGFERITGYSAADLLGTRGLARLRPRDQAGREVRFERWAEEGTKLPPVLEVLNPEG
                     EHCWIVCSYSMVPALSDGQPRRMVITSRDITRERELERAGETLRKSAARFRALVQNSS
                     HLVVILDAGGGISYASPAFHRALGYPDAAEAELEQNLFDLVHPDDVQPLRTRFGEHLA
                     LASAAGAGALEFRFLAADGTWRHLEALANNLLDDPAVGGIVFNAHDVTERKRAEALLA
                     GQAHVLDLIARDAPLMEVATALARLVEREADGARCAVLLLDDSGEALTVAAAPSLVDV
                     GLHEADGLIVGPAAGASGTAAWRRESVICGDVASDPLWADKRQVALPRGLRAAWAMPI
                     MAPDGDRALGTVTLYFDEPREPQPFELQLLDLAVHLADIAVERAEAQAKLAHQAAHDA
                     LTGLPNRVFFQDRAAHALARTRRSRSAVAVLFLDLDRFKCINDSLGHEAGDRLLVELA
                     HRLQEVVRPGDTVARFGGDEFIVLCEGIVDEAHALSIAERVGEVAATPFALGGGEVFV
                     SLSVGIAMASGAGARPDTLVENADAAMYRAKARGGNRCEMFDRAMRSRAKRRLTLHSG
                     LHRAVERGEFQVVYQPIVALGSRRPVGAEALRRWSAGRCRTAA"
     gene            8109..8786
                     /locus_tag="VF995_02795"
     CDS             8109..8786
                     /locus_tag="VF995_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9342530.1"
                     /translation="MPPGEFIGLAEETGLIMPIGTYVLREACRRAGQWHAAGAPGDNG
                     DPFCIKVNLSARQFVHPDLVEVIAGILNETSVDPASVYLEITESVLMEDAESTVTALR
                     DLKALGVSLAVDDFGTGYSSLTYLKRFPVDELKVDRSFVAGLLADPEDAAIVAAVVNL
                     AHTLGLKAVAEGVESAGQLTRLRDLGCDLGQGYYFGRPMPAESMATRLGLGAASNGSP
                     GRAEVLT"
BASE COUNT         1213 a         3166 c         3224 g         1295 t
ORIGIN      
        1 cgagcgggcg ttcccccccg gagcggaggt cgtacgtccg cttccgctca ccttcccgcc
       61 gagggggacg ccgaagcacc gaaatcgcgc gagaggtgcc gccgcggcag gcgcggagct
      121 gagaggggtg gagggtgacc gaggggactc gaacccccga ccttcgggac cacaacccga
      181 cgctctaacc aactgagcta cgatcaccat ggccaagcga ggggaaggat acccggctcc
      241 gagccgcgtg gccagcgagc gcgtcgactc gcgtcagcgt tggcagcggc gctggcgcgg
      301 gcccgccggc tgggaggggg gcgcgatggt caacgagatc aagaccgagc ggttgtggct
      361 tcgccagctc ccggttgagg aggcgcgggc gctgctcaac ggcacagcgc agccggagtt
      421 gccctggatg gccgggtatc cgattgaggg gtcgatgatc gcggccgaag cctacgtccg
      481 cgccatcgac ggcggccagc aggtcggtcc gtacggcttg taccagatcg tcgtcaccga
      541 ggagggcctg gtcgtgggcg acatcggctt ccacggacca cccgatccgg tccggtcggt
      601 gaccatcggg tacgggctcg ccgcgcccgc gcgagggcac gggtacgtga ccgaggcgct
      661 gcgggcgctg atcgcctggg cgatggccca gccggaggtg cggcgggtcg aaggcgacgc
      721 ggtccacgcc aacctggcgt cgcagcgcgt gatggagcgc gcgggcatgc gctatgtcgg
      781 cagcgacgtg cagttgcgct actaccgcta cccgtgatca tcctggaggg cgagccgtgg
      841 agcggcgcgt gacgtcaacc ccgccatccc cggaggatgc tcatgaatca gacgccgacg
      901 cgcgtcttcc tcgtcaccgg cgcctccagc gggatcgggg aggcggccgc ccgccggctg
      961 gtcagggagc ctgccgccca cgtgatcctg ctggcgcggc ggcgcgagcg gctggaggca
     1021 ctcgccgccg agctgaccgc cgccggtggc agcgccagcg tcatcggcat cgacctcacc
     1081 gactacgacg cgcccggcat ggtcgcggcc gtgctcgagc gtgaccacgg ccgcctcgac
     1141 ctgctggtca acaacgccgg cgtgggtggg cgcggcagct tcgccgagag cggcttcgcc
     1201 gaggtgcagc ggaccatgcg ggtcaacttc ggggccccgg tccgcctcac cgaggcgctg
     1261 ctgccgctgc tgcgccgctc tgcgccgagc gcgatcgtga acgtcgccag cgtcgccggc
     1321 cgggtcgggc ggcccggcac cagcgcctac tcggccagca agttcgcgtt gatcggctgg
     1381 agcgagtcgc tgcagctgga ggagcgcccc cacggcgtcc acgtcggcct ggtggtgccc
     1441 gggttcgtct ccaccgaggg gttcccgcag cgggagctgc tggcccaccg ggcgacccgc
     1501 tggctggtca gcaccaccga caaggtcgcc gacgcgatca ccgaggtcgg cctggggcgc
     1561 aaggcggaac gctacgcacc gcggccctgg gcggtggtgc cgttcctgaa ggtcctgctg
     1621 ccggggctct accgccgcgc ggtcggcggc ggccgcttca ccgccgccgc caagcgctga
     1681 gccagcccgg tccgggtgct gggccgggga cgttcagagc agtgggtgga gggcgtcccg
     1741 gatctggacc gccgtgaccg ggcccatgta gcgggcgcgg atcacgccgc ccttgtcgat
     1801 cacgaacacc cacggctcgg tctggaagcc ccaggcgctg aacgctttcg ccggcttgct
     1861 cgggtcgcgg gtcgggaact cctcgacgtg gatgaagttc gccttggccg cgccaacctg
     1921 gtggaaggcg acgagctgct cgtcgaccac cgggccgcac atgcggctct ggcacaacag
     1981 cggagtggcg aacgagatca ccgtgggtct gccggagcgc agcgccttgt ccagcgagat
     2041 ggagtgcatc gtgcagggag ggttgcgggt gcagatcttg gcccgcgcgg ccgtgctggc
     2101 cgccaccggg gtcggctccg acagcgcctt gctgccgatc gcgccgacga ccttctcgct
     2161 gaccggggcg ccgccctggc cggcaacgtg cgtgccgttg aggtcggcga ccgccacgat
     2221 cagccagttg cccgccgacg gcgcctcgac ctcggcgccg tagaagctgg tcagctcggt
     2281 cgcgggagac ttgtcgccgg tcaagccgta ggcgctcatg tggaacgagg tggccgggaa
     2341 cgggccgagt gccttgctgg tgtcgtcctt ggcgagccag acctggggag agccgccgct
     2401 gatcagctgg ttgtccgggg tgacgaggcc gaaggtgaag aggctccggc cgactggaag
     2461 ggggttctgc gcgtcagcgg tgccgagcag ggagagctgc gtggcgccgg ccgccagcgc
     2521 cttcagcgat ccggcgggcg ccgtgccagt cgaggaggag ccgccgccct tgctgccgca
     2581 ggcgctgacc agggccgaga cccccagggc gccggctccc agcgtggccg agcgcagcag
     2641 gtcgcggcgc gagagcaggc cggggtcgcg gtcggagcgc cggtgatcgc tcatcttccc
     2701 ctccgcgaag gtgcggcaac gaggtggcgg tgcacaacgc accatacgaa ctggaagcgt
     2761 agtggagttc acccgattcc gcgcggcgac gccggaccgt ccgggcccgc agccgcgaca
     2821 gcgaccgcga cacagcgacc gctacacagc gaccgctaca cgccgtcgtt gtcgttgtcg
     2881 tccggccagg gggcgggcac gccgcctggg ccggccggga ccacctggtg ctccagggcg
     2941 aaggcgaccg cttccagttt ggagtggacg ccgagcttga cgagcacgtt ctggacgtgg
     3001 gtgcggacgg tgttgaggga gaggaagcac tcctcggcga tacggcgatt ggagtagccg
     3061 ttggcgagca tggtgaggat ctgccgctcg cgcgcgctga gggtgcgtac ccgcagttcc
     3121 acgctggggt cgcggggctg gcgtgccgcc ccggcggggg tgaagaagcc gccctcgccg
     3181 tcgaccaggc ggcggatcat gctggcgacc tggcggccgg aggtgtcctt ggcgaggaag
     3241 ccgtcggcgc cggcctgggt ggcggcggtg atggtctccc ggcgggtgtc ggcggagagc
     3301 accatcacct tggtctgcgg cgccttcacc ttggcgtcgc gcagcacctg ggtgaggctg
     3361 gtgttgggca tgtgggcgtc gagcagcagc acggtgggcg cgaggctgct gatcagctcc
     3421 agggcctcgc cgccgtcgcg ggcgacaccg acgagttcga tgtcgtcctc ggcgtcgagc
     3481 accatcccga ggccttccgc gaaggcctgg tgatcgtcgg caagcacgag tgtgatcatg
     3541 tcccccccag gacatccccg gctatccaat aggcacgcac gccacggctg ctgacatccg
     3601 gatgtggcgg cgaccaccag tagcctacgg cacctggttg accgcgctta tccggagaag
     3661 tgatgagttc gggtcccggc gtgcgaccag agcttccgag actggtgagt tttgctggta
     3721 ttgccggtct gaagccgttc acctgctgct tcttggctgg cattggtatg ctccgtagtc
     3781 tagcttgcgt caaataccgt agaagatgtg cctaccgatc acgcatgcaa tgcaaggtgg
     3841 actgcggcaa cctgttgatt ctgcggtgac gacatgtctg ctggccgggg gaggccgcag
     3901 cgttcgaagg gacagcttga tggctcgcgt gttcctggcc gactgccagc cactgttcaa
     3961 cgaggcgctg gaggcgttga tcgcccggga tggccagaac gaggtcgtcg ggcaggcttc
     4021 gtccgctccc gaggtcaccg acgcggtcgt ccacctcaaa cccgatctgg tggtgctgga
     4081 cgccgacctg gcgctggcca gccggccctc actggtcgag gcgatcctcg acgagctgcc
     4141 cgccacccgg gtcatcctgc tggcgcagga caccgacctg gagctgctgc tgatggcgat
     4201 ccgtgccggc gccctcggcg tcgtgggcaa gaagagcggc acccagaccg tgctccgtgc
     4261 ggtccaggcg gtcctcgacg gcgagggcgt ggtcccacgg gcgatgcttc ccgcgctgtt
     4321 ccggcgcctg ctggacctcg gcgaccgcgc cgccgacacc ccgctgaacc gcctctcgcc
     4381 gcgcgagcag gaggtgctgg cgctgctcgg ccgcggctgg agcaatgccc gcatcggcaa
     4441 ggagctgttc atcagccccc acacggtccg cacccacgtg cagaacgtcc tccagaaact
     4501 ggagatgcac tccaagctgg aggcggccac cttcgcgatg cagcacgagc tggccgcgaa
     4561 gctgccggcg ccagggtcag ggccgggcgc gccagagcag caccagggcg acgccccccg
     4621 acctccggca cgcaagccgg cacgcaagca gagcacgggt cgataaacgc ggcgagagcg
     4681 cacaatctgg gcgtcttcca ctgtgtcttt gggaagctct gcgcgtaagg ccatcatatc
     4741 cgtcagtatt ttgacgtagg gctggctatt ttgtcttggg ctttggtgga gttccgcgca
     4801 ttatgaccgt aacaagccgc cggagccttc cgagcggcta gtgacgcatc tcgctacagc
     4861 cgaaccgggc gaaagggatg gggggcgatg gacgaccgga ctgtggcgac cgctgccgct
     4921 ccagcgacga cagcgaccgc ctccgctccg cctccggccc gggatgcgat ccgggacggg
     4981 gagggcttcc cccgttcggt gctgctgctg gtcgcgatcc tggcggtgct cgcgggtgct
     5041 gccgcgctca ccgaccgggc gcccggcagc gggccggtcg ccaccatcgc cgtgatcggg
     5101 ctgctggcgg cgctcaccgc cgccgggtcg ctgcacatcg acttccgcta ccacgacgag
     5161 atcgaggcgc tggagctgtt cgaatcggtg ctgctggtgg ccgtctacgc gctctccggg
     5221 cggctggtgg tggtggtgac cgccgtggcc gccgccctgt cggagtcgct gctgcgcgtc
     5281 cgcccgctca aggccgcctt caacgtggcg cagtggtcgg tcgcggcggc cgcgggcagc
     5341 atcgccttcg cggcgctgcg cagcggcacc gcgctcaccc cccgcaacct gaccgccctg
     5401 ctggccgcga tggtggtggt cgcgctggtc aaccactgca gcctggtggt ggtcttcggg
     5461 ctggtccgcc gcgagcgcct cggccgggtg ctgcgcggcc tcgccgaggt gatcctgccc
     5521 ggctgggtgc tcggcggcgc catcaacctc gccttcggcg tcctgtttct cgccacctgg
     5581 atctggtggc cgttcgcggc gccgctgttc ctgctgccgc tcggcatcct gcactgggcc
     5641 gcgcgcgtct acgcggcgat ccgcatcgac cgcacccggc tcaccggcat gcaggaggcc
     5701 acccacctgc tggtctcctc gctctacccg agcgaggcga tgcccgcctt cctcgccgcc
     5761 gtgcgccgct gcttcgaggc cgaagccgtc gagttggtgc tggccggcga ccccagcggc
     5821 gatgaggcga ccctgtggcg cagcgccgac ggcgacccgg gcgaggtcgg ccgcaccacg
     5881 ccggagtcgt tcggcctcgc ggtgctccgg ctcggcacgg cggttcgggc gcgggccggc
     5941 ggggacggcg agctggccgc gccgctcgcg ctggctggct ggcgcgactg ccttgccgcg
     6001 ccgctgcggg ccggcgccgc cgccgggatg ctgtgcacct acaaccgcaa cggcctggaa
     6061 ggcttcgagg agggcgagct ggccgtcctt gaggcgctcg cggcggaact gggcactgcg
     6121 ctggaaaaga gcgacctgct ccagacgatg ctggaggagc gtgccaagct ctccgagatc
     6181 gtcgaccaca cctccgacgg gatcgccgcg ctcgaccccg acggcaccgt gtcgagctgg
     6241 aaccccgggt tcgagcggat caccggctac tccgccgccg acctgctcgg cacccgcggc
     6301 ctcgcccgcc tgcggccacg agaccaggcc ggccgcgagg tgcgcttcga acgctgggcc
     6361 gaggagggca ccaaactccc cccggtgctg gaggtgctca accccgaggg cgagcattgc
     6421 tggatcgtct gctcctacag catggtcccg gcgctgagcg acggccagcc gcgccgcatg
     6481 gtgatcacct cccgcgacat caccagggaa cgggagctgg agcgcgccgg ggagaccctg
     6541 cgcaagtcgg cggcgcgctt ccgcgccctg gtgcagaatt cctcccacct ggtggtcatt
     6601 ctcgacgcgg gcggaggcat cagctacgcc agcccggcct tccaccgcgc cctcggctat
     6661 cccgacgccg ccgaggccga gctggagcag aacctcttcg acctggtcca ccccgacgac
     6721 gtccagccgc tgcgcacccg cttcggtgag catctggcgc ttgcgagcgc cgccggcgcg
     6781 ggcgccctgg aattccgctt cctggccgcc gacgggacct ggcgccacct ggaggcgctg
     6841 gccaacaacc tgctcgacga ccccgccgtc ggcgggatcg tgttcaacgc ccacgacgtc
     6901 accgagcgca agcgcgccga ggcgctgctg gccgggcagg cccacgtcct cgacctgatc
     6961 gccagggacg cccccttgat ggaggtggcc accgccctcg cccgcctggt cgagcgcgag
     7021 gctgacgggg cccgctgcgc cgtgctgctg ctcgacgaca gcggcgaggc gctcacggtc
     7081 gcggcggcac ccagcctggt cgacgtcggc ctccacgagg cggacgggct gatcgtcggg
     7141 cccgccgccg gagccagcgg caccgccgcc tggcgccggg agtcggtgat ctgcggcgac
     7201 gtcgccagcg acccgctgtg ggccgacaag cgccaggtcg ccctgccgcg cggcctgcgg
     7261 gccgcctggg cgatgccgat catggcgccg gacggcgacc gcgccctcgg cacggtgacg
     7321 ctctacttcg acgagccacg cgagccgcag ccgttcgagc tgcaattgct ggacctggcc
     7381 gtccacctcg ccgacatcgc cgtcgagcgt gccgaggccc aggccaagct cgcccaccag
     7441 gccgcccacg acgccctcac cggcctgccc aaccgggtct tcttccagga ccgcgccgcc
     7501 cacgccctgg cgcgcacccg ccgcagccgc tccgcggtgg cggtcttgtt cttggacctg
     7561 gaccgcttca agtgcatcaa cgactccctc ggccacgagg ccggcgaccg cctgctggtc
     7621 gagctggccc accgcctgca agaagtggtc cgccccggcg acacggtggc ccgcttcggc
     7681 ggcgacgagt tcatcgtgct gtgcgagggc atcgtcgacg aggcccacgc cctgtcgatc
     7741 gccgagcgcg tcggcgaggt cgccgccacc cccttcgcgc tcggcggcgg cgaggtgttc
     7801 gtcagcctca gcgtcggcat cgccatggcc agcggcgccg gcgcccggcc cgacacgctg
     7861 gttgagaacg ccgacgccgc catgtaccgg gccaaggcgc gcggcggcaa ccgctgcgag
     7921 atgttcgacc gcgccatgcg ctcccgcgcc aaacgccgcc tgacgctcca cagcgggctg
     7981 caccgcgccg tcgagcgggg cgagttccag gtcgtctacc agccgatcgt cgccctcggc
     8041 agccgccgcc cggtgggcgc cgaggcgctg aggcgctggt ccgctgggcg ctgccggacc
     8101 gcggcctgat cccccctggc gagttcatcg gcctggcgga ggagaccggc ctgatcatgc
     8161 cgatcggcac ctacgtgctg cgcgaggcct gccggcgcgc cggtcagtgg cacgccgccg
     8221 gcgcgccggg agacaacggc gacccgttct gcatcaaggt gaacctgtcc gcccgccagt
     8281 tcgtccatcc cgacctggtc gaggtgatcg ccggcatcct caacgagacc agcgtcgacc
     8341 cggccagcgt ctacctggag atcaccgaga gcgtgctgat ggaggacgcc gagtcgaccg
     8401 tgaccgccct gcgcgacctc aaggccctcg gcgtgtcgct cgccgtcgac gactttggca
     8461 ccggctactc gtcgctgacc tacctgaagc ggttcccggt cgacgagctg aaggtcgacc
     8521 gcagcttcgt ggccgggttg ctcgccgacc cggaagacgc cgccatcgtc gccgccgtcg
     8581 tcaacctcgc ccacaccctc ggcctcaagg cggtcgccga gggggtggag tcggccgggc
     8641 agctcacccg cctgcgcgac ctcggctgcg acctcggcca ggggtactac ttcggccggc
     8701 cgatgcccgc cgagtcgatg gcgacgcgcc tcggcctggg cgcggcgtcg aacgggtcgc
     8761 cggggcgggc cgaggtgctg acctagtggc ggccacctgc gtggcagcca cgtaaccgtc
     8821 aggctgaggg tgagggcacc aggcgggcgt cgaccaccag gttgtcgcgg tagctgtggc
     8881 gggcgccgtc gaagctgc
//
LOCUS       DASWDC010000085         4472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_760726, whole genome shotgun sequence.
ACCESSION   DASWDC010000085 DASWDC010000000
VERSION     DASWDC010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760726"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VF995_02800"
     CDS             <1..608
                     /locus_tag="VF995_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEX9342531.1"
                     /translation="FRPAADGGVLDRAGVVDYATGPVAPGVFVVARSNEPVVIEEMAY
                     LSMGPGPYFSFYRPYHLASIEAPLSVAEAVLDRAPSLVPECWMAEVIALAKRPLKAGE
                     RLGGIGGDHYYGVVDSAAVAKAEGLVPIGVVAGATVTRNVPVDQPLTWDDVELDPDSV
                     IVSLRRLQDRLFDAPQAHAQARNGIDPALPQALSAATTHIR"
     gene            630..1631
                     /locus_tag="VF995_02805"
     CDS             630..1631
                     /locus_tag="VF995_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEX9342532.1"
                     /translation="MVRASRSTDELAKVAQLYYLDDLSQQEIAERLGTTRSNVSRMLK
                     AAKQAGLVEIRVVHPTQRDRELEAALKRRFRLQEARVAPFDPAAGAGAAAGHGQQPLA
                     QVGTLAASWLDETLRDGMRLGLSWGISLQAMVDEVTPERRHAVELMQLVGGLSALQAG
                     ASGHELIRGLAERLGADYAYLHAPALFESQAACRTLLEEPSIRDYLERARQVDVALVG
                     IGARGVGSSAAIVEALNLSPAERAELEASGFVGDVCARYFDLNGQEVRSAARNRVLAV
                     SLDDLRRIPTVVGVLAGREKALGTLGALRGQLLDVLVCDEAAARSILEFDGAGPPGT"
     gene            1749..2678
                     /locus_tag="VF995_02810"
     CDS             1749..2678
                     /locus_tag="VF995_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX9342533.1"
                     /translation="MWTIRHFEEAVDAMFARGLMHGTMHLSIGQEAVATGACRALRTD
                     DLITSTHRGHGHCIAKGADPGRMMAELLGSEAGYCRGRGGSMHIADVASGNLGANGIV
                     AGGIPIAVGASLAHRLQGSDRVTVCFFGDGAANEGAFHEAANLAAIWDLPVVLICENN
                     HYGMSMPTTRAFRVEQIAARAAAYGFGGETVDGNDLQAVSDAVDEAVARARAGAGPTL
                     IEAVTYRWKGHSKSDQNRYRTREEIADWKQRDPIARFEAATRDAGTLDDAGLAAIRAT
                     ARDTVREAIRAGRAGPPPSPDDLLPAVYAQGVV"
     gene            2680..3684
                     /locus_tag="VF995_02815"
     CDS             2680..3684
                     /locus_tag="VF995_02815"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX9342534.1"
                     /translation="MPAATTVTMTYAEAVRAALAEELEADPRVVLLGEDIGVYGGAFG
                     VSQGLLDRFGAERVRDTPISEEGIVGAAVGLALCGMLPVAEMQFSDFTANAMDQLVNQ
                     AAKLHFMLGGAVRVPLVLRLPAGSGTGAAAQHSQSQEAWYVHTPGLKVVMPSNPADAK
                     GLLHSAIHDPNPVVFFEHKLLYRTSGPVPPEAGFRVPLGKAAVPREGRDLTIVATGVM
                     VSRSLAAADELAGEGVEACVVDPRTLSPCDDATILERVSATGRVLLVQEAPRTAGFMA
                     EVAARIAESSAFSDLAAPVARLCGLDTPIPYNPDLEKAAVPQVPDIVQAASRLLKGND
                     "
     gene            3684..3956
                     /locus_tag="VF995_02820"
     CDS             3684..3956
                     /locus_tag="VF995_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX9342535.1"
                     /translation="MRELQVPMPKLSMTMEEGELAAWHKHEGDVVEAGEILCEVASDK
                     AEIEVESPVDGTVVTLHAAEGDVVKVGAPIATLATEADDLVGDLLD"
     gene            complement(3953..4270)
                     /locus_tag="VF995_02825"
     CDS             complement(3953..4270)
                     /locus_tag="VF995_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342536.1"
                     /translation="MEHVHLVVLNCANCHGEQLHEVVYAGRLLAATVCSNCGHEIAKD
                     LPTLRRAYVSDVEHRVLTKPMRMLRAAIGHPLSFARALPGAVITKPAKLEHELRVLQG
                     RRD"
     gene            complement(4243..>4472)
                     /locus_tag="VF995_02830"
     CDS             complement(4243..>4472)
                     /locus_tag="VF995_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX9342537.1"
                     /translation="AYEAAASDGFAGSDTAACVERYTEVPVRVVPGDPGNLKVTFPDD
                     LFIAERLLAAQPRTSGHRSRGTPWSMSISSF"
BASE COUNT          648 a         1715 c         1521 g          588 t
ORIGIN      
        1 tcttccggcc ggccgccgac ggcggcgtgc tcgaccgcgc cggggtggtc gactatgcca
       61 ccgggccggt cgcgccgggg gtgttcgtgg tggcgcgcag caacgagccg gtggtcatcg
      121 aggagatggc ctacctcagc atgggccccg gcccctactt cagcttctac cgcccctacc
      181 acctggccag catcgaggca ccgctgtcgg tggccgaggc ggtgctcgac cgcgccccct
      241 cgctggtccc cgaatgctgg atggccgagg tgatcgccct ggccaagcgg ccgctgaaag
      301 ccggcgagcg cctcggcggc atcggcggcg accactacta cggcgtggtc gactcggccg
      361 ccgtcgccaa ggccgagggc ctggtgccga tcggggtcgt cgccggcgcc accgtgaccc
      421 gcaacgtgcc ggtcgaccag ccgctcacct gggacgacgt cgagctcgac ccggactcgg
      481 tgatcgtcag cctgcggcgg ctccaggacc gcctcttcga cgccccgcag gcgcatgccc
      541 aggcgcggaa cggcatcgac ccggcgctcc cgcaggcgct gtccgccgcc acaacgcaca
      601 tccgttagca ataccgcaca atgttgccta tggtccgagc gagcagatca actgacgaac
      661 tggccaaagt ggcgcagctc tactacctcg acgacctctc ccagcaggag atcgccgagc
      721 gcctcggcac gacccgctcc aacgtgtcgc gcatgctgaa ggccgccaag caggccgggc
      781 tggtggagat ccgtgtcgtc cacccaaccc agcgcgaccg cgagctggag gctgccctca
      841 agcgacgctt ccgcctccag gaggcccgcg tcgcaccctt cgacccggcc gccggtgccg
      901 gtgccgcagc cggccacggc cagcagccac tcgcccaggt cggcacgctc gccgccagct
      961 ggctcgacga gacgctgcgc gacggcatgc ggctcggcct gtcctggggc atctcgctgc
     1021 aggcgatggt ggacgaggtc acccccgaac gccgccacgc cgtcgagttg atgcagctgg
     1081 tcggcggcct gtcggcgctg caggccggcg ccagcggcca cgagctgatc cgcgggctgg
     1141 ccgagcggct cggcgccgac tacgcctacc tgcacgcgcc cgccctgttc gagtcgcagg
     1201 ccgcctgccg caccctgctg gaggagccct ccatccgcga ctacctggag cgcgcccgcc
     1261 aggtggacgt ggcgctggtc ggcatcggcg cccgcggggt cggctcctcc gccgcgatcg
     1321 tggaggcgct caacctgagc ccggccgagc gcgccgagct ggaggcctcc ggcttcgtcg
     1381 gcgacgtctg cgcccgctac ttcgacctga acggccagga ggtccgctcc gccgcccgca
     1441 accgggtgct cgccgtcagc ctcgacgacc tgcgccgcat ccccacggtg gtgggcgtgc
     1501 tggcgggccg cgagaaggcc ctcggcacgc tcggcgcgct gcgcggccag ctgctggacg
     1561 tgctggtctg cgacgaggcg gccgcccgct cgatcctcga attcgacgga gccggcccac
     1621 ccggcacctg aaacctggac ctcaacctca acctcaattt gagcctcaac cgcgatacga
     1681 cgaaaggaag ttcccgcgtg accaagctcg ccaccgaccc agatgccgcc cgtcgtgccc
     1741 tgaccaccat gtggacgatc cggcacttcg aggaagcggt cgacgccatg ttcgcccgcg
     1801 gcctgatgca cggcaccatg cacctgtcga tcgggcagga agcggtcgcg accggcgcct
     1861 gccgtgccct ccgcaccgac gacctgatca cctccaccca ccggggccac ggccactgca
     1921 tcgccaaggg cgccgacccc ggccggatga tggccgagct gctcggcagc gaggccggct
     1981 actgccgcgg ccggggcggc tcgatgcaca tcgccgacgt cgccagcggc aacctcggcg
     2041 ccaacggcat cgtcgccggc ggcatcccga tcgccgtcgg cgccagcctc gcccaccgcc
     2101 tccagggctc cgaccgggtg acggtctgct tcttcggcga cggcgccgcc aacgagggcg
     2161 ccttccacga ggccgccaac ctggccgcca tctgggacct cccggtggtg ctgatctgcg
     2221 agaacaacca ctacggcatg tccatgccca ccaccagagc cttccgtgtc gagcagatcg
     2281 ccgcccgcgc cgccgcctac ggcttcggtg gcgagaccgt cgacggcaac gatctgcagg
     2341 cggtcagcga cgcggttgac gaggccgtgg cgcgggcccg cgccggcgcc ggcccgaccc
     2401 tgatcgaggc ggtcacctac cgctggaagg gtcactccaa gagcgaccag aaccgctacc
     2461 ggacccgcga ggagatcgcc gactggaagc agcgcgaccc gatcgcccgc ttcgaggccg
     2521 ccacccgcga cgccggcacc ctggacgacg ccggcctcgc cgcgatccgc gccaccgccc
     2581 gcgacaccgt ccgcgaggcg atccgcgccg gccgcgccgg cccaccgccc tcacccgacg
     2641 acctgctgcc agcggtctac gcgcaggggg tggtctgaga tgccggcggc cacgaccgtg
     2701 accatgacct acgccgaggc ggtccgcgcc gcgctcgccg aggagctgga ggccgacccc
     2761 agggtggtgc tgctcggcga ggacatcggc gtctacggcg gcgccttcgg cgtcagccag
     2821 ggcctgctcg accgcttcgg cgcggagcgg gtccgcgaca cgcccatctc ggaggagggg
     2881 atcgtcgggg ccgcggtcgg gctggcgctg tgcggcatgc tcccggtcgc cgagatgcag
     2941 ttctccgact tcaccgccaa cgccatggac cagctggtca accaggccgc caagctgcat
     3001 ttcatgctcg gcggcgcggt ccgggtcccg ctggtgctgc gcctgccggc cggctccggc
     3061 accggcgcgg ccgcgcagca ctcccagagc caggaggcct ggtacgtgca cacccccggc
     3121 ctcaaggtgg tgatgccgtc caacccggcc gacgccaaag gcctgctgca cagcgccatc
     3181 cacgacccca acccggtggt gttcttcgag cacaagctgc tctaccgcac ctccggccca
     3241 gtgccgcccg aagcgggctt ccgcgtcccg ctggggaagg cggcggtgcc acgcgagggc
     3301 cgcgacctga ccatcgtcgc caccggcgtg atggtctcgc gttcgctggc agccgcggac
     3361 gagctggccg gcgagggtgt ggaggcctgc gtggtggacc cccgcaccct ctccccgtgc
     3421 gacgacgcga cgattctcga gcgggtcagc gccaccggcc gggtcctgct ggtccaggag
     3481 gcgccccgca ccgccgggtt catggccgag gtcgccgccc gcatcgccga gtccagcgcc
     3541 ttcagcgacc tggccgcgcc ggtcgcgcgg ctgtgcggcc tcgacacccc gatcccctac
     3601 aaccccgacc tcgaaaaggc cgccgtgccg caggtcccgg acatcgtcca ggcggcaagc
     3661 cggctgctga aagggaacga ctgatgcgtg aactccaggt gcccatgccc aagctctcca
     3721 tgaccatgga ggagggcgag ctcgccgcct ggcacaagca cgaaggcgac gtcgtcgaag
     3781 ccggcgagat cctctgcgaa gtcgcctccg acaaggccga gatcgaggtc gagtccccgg
     3841 tggacggcac cgtggtcacg ctccacgccg ccgagggcga cgtcgtcaag gtcggcgccc
     3901 cgatcgccac cctcgccacc gaagccgacg acctggtcgg cgacctcctg gattagtccc
     3961 ggcggccctg cagcacccgc agctcgtgct ccagcttggc cggcttggtg atgaccgcgc
     4021 cgggcagggc gcgggcaaac gacagcgggt ggccgatggc cgcgcgcagc atccgcatcg
     4081 gcttggtcag cacccggtgc tcgacgtcgc tgacgtaggc gcggcgcagg gtggggaggt
     4141 ccttggcgat ctcgtggccg cagttggagc agactgtggc ggccagcagc cggccggcgt
     4201 agacgacctc gtgcagctgc tcgccgtggc agttcgcgca gttcagaacg acgagatgga
     4261 catgctccat ggcgtacctc gtgaccggtg gccggacgtt cgcggttgcg cggccagcag
     4321 gcgctccgcg atgaacagat cgtcagggaa ggtgaccttg agattccccg ggtcgcctgg
     4381 caccacccgc accggcacct cggtgtagcg ctccacgcag gccgcggtgt ccgagccggc
     4441 gaacccgtcg ctcgccgccg cctcataggc cg
//
LOCUS       DASWDC010000086        12179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_18637, whole genome shotgun sequence.
ACCESSION   DASWDC010000086 DASWDC010000000
VERSION     DASWDC010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12179
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18637"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VF995_02835"
     CDS             <1..944
                     /locus_tag="VF995_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009069194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX9342538.1"
                     /translation="DAMDDDTLTEFLIGGATKRELLERAEAPPSLVFHTIGMDDFLLA
                     PLPNHLFTRDASCWIGDGVAINSMRKKARRRETIHYEAIYRWHPLFSSQDFGIWSQGT
                     EGGVATVEGGDVLVIGNGAVLVGMSERTTPAGVERLARRLFEAGAARRIVALELPKAH
                     AFMHLDTVMTMVDGDTFTMYAGLPPLAAHTIEPGDAPGDAGPGSGPAKELKVTDHPPE
                     PAAMRGAIAATLGLDDVKILTATQDVRSAEREQWDDGCNVLAVAPGVVMAYERNVTTN
                     TWLRRNGVEVIIVRGGELGRGRGGPRCMSCPIEREAA"
     gene            1007..2455
                     /locus_tag="VF995_02840"
     CDS             1007..2455
                     /locus_tag="VF995_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005485523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9342539.1"
                     /translation="MLRTKPVEELVGESGRGHEQGAELRRSMGLLQLILLSVGATLGT
                     GIFVILGDAAPLAGPAVVLSFLLAAVTALFSALSYAELAGTIPVSGSSYSYAYATLGE
                     LAAWVSGWCLMAEYGVSVSAVAVGWGQYLNELGRSTLGFSLPAAIANPPGEGGVVNLT
                     AIVVVAAAALLLMRGTSESAAVNTVMTLIKLAVMVFFCAVACTAFRAHNLRPFAPLGF
                     AGISAAGATVFFSYIGFDAASTAGEEARNPKRDLPMAIIISLGIVTLVYAAVALAAVG
                     VTPWKQLGGLQAPLASVLSDATGSTWPAAVLSFGAVIAIASVVLTVMYGQTRILFAMA
                     RDGLVPRVFDRVSPRTQTPVANTLIVAGFIAALAGFFPLGHLADAASIGTLAAFAIVN
                     FGVLVLRRTRPDAPRSFRVPFYPLTPILGVCLCAWLMAKFDRVTWVAFVLWTVAGLAA
                     YAAYGYRHSRLGQAAAAAATPDARTRGASGTR"
     gene            complement(2412..4013)
                     /gene="aceB"
                     /locus_tag="VF995_02845"
     CDS             complement(2412..4013)
                     /gene="aceB"
                     /locus_tag="VF995_02845"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX9342540.1"
                     /translation="MASLRPSGVEVHGPAQQKYPQILSDEALAFVAELQREFNPTLEE
                     LLAKRRERQAAFEAGELPDFPPETASIREGDWRVAPYPADLADRRVEITGPTDRKMLI
                     NALNSGARVFMADFEDANAPTWDNLFGGQANLIEAIERTISFDGPDGRHYQLNDEVAV
                     LMPRPRGWHLPEKHLLVDGQPAAGALVDFGLYFFHNATRLLGKGTGPYFYLPKLESHL
                     EARLWNDVFVLAQDHLGIPRGTIRATVLIETITAAFEMDEILYELREHSSGLNAGRWD
                     YMFSVIKKFRTRPEFTLPDRNQVTMTVPFMRAYTELLVRTCHHREAFAMGGMAAFIPS
                     RRDPEVNRVALAKVRDDKRREAGDGFDGTWVAHPDLVQAAAECFDEVLGTRSNQIERK
                     RDDVQVRASDLLNVPATPGDVTEEGLRNDVSVAIQYISSWLRGSGAAAISNLMEDVAT
                     AEIARSQVWQWVHNGVKLSDGREVTPDLVRQVEAEELDKLRQTFGEEVFAASRADEAQ
                     ALFEQVALADDFIEFLTLPAYEHLE"
     gene            4179..4385
                     /locus_tag="VF995_02850"
     CDS             4179..4385
                     /locus_tag="VF995_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018542743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9342541.1"
                     /translation="MNNRVRVLRTERGLSQGELAEAMGVSRQTINSIETERYTPSLPL
                     AIALAHYFAKPVEEVFNANGTQST"
     gene            4366..4794
                     /locus_tag="VF995_02855"
     CDS             4366..4794
                     /locus_tag="VF995_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342542.1"
                     /translation="MAPNQREPRGLVSRLCGGLGGWGMFLFSLALGAVMWTASWIGGH
                     PMLGLAMFGIMAAFGAVFVVGRRSETIRLMAAGRHADERWRSIDLRATAFMGIVLGLA
                     VIVAFLWAIAHGRDGQPYAPLGAIAGISYLIAIIYLRWRS"
     gene            complement(4823..5446)
                     /gene="pdxH"
                     /locus_tag="VF995_02860"
     CDS             complement(4823..5446)
                     /gene="pdxH"
                     /locus_tag="VF995_02860"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX9342543.1"
                     /translation="MAGLEIAEVDPDPIGQFERWFKEAVAAALPEPTAMALATATPAG
                     RPSVRMVLLKGVDQRGFVFYTNHESRKGQELAANPLAALTFWWPPPVHRQVRVEGRVE
                     RVPLEESEAYFRTRPVGSRLGAWASPQSSVIPGRDVLERRYETLAARYGGDAADAEVP
                     LPPFWGGYRLTPGVIEFWQGRENRLHDRLRYTRERPTAPWTIERLAP"
     gene            complement(5579..8122)
                     /locus_tag="VF995_02865"
     CDS             complement(5579..8122)
                     /locus_tag="VF995_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9342544.1"
                     /translation="MLKATLKGLAAHKLRMSLTALAIVLGVGFVAGTFVLTDTINKTF
                     DNLFNQVTQGVDVAVRTNATFNAQGGQTRAPMPSSVLDQVKGVQGVAAAEGSVNGYAQ
                     FIGSNGKPVTTGGAPTLGVSLSSVPQLQSATTLRGGARPTSSDQVAIDAATAKKQGFK
                     VGDRVKILFQGPPREFTVSGILGFGTADNLAGATLAGFDLKTAQQVLNRVGTYDEVDA
                     VAASGVSPATLRDRVKAVVDPKFQVLTGKQLAQDDSKTVGSFTKFINIALLAFAFVAL
                     FVGSFIIVNTFSIILAQRTRELALLRCLGASRRQVLRSVLAEAAIVGLAGSVVGIGFG
                     ILVAIGLNNAFKALGVDLPQTTPVIAPRTIIVALVVGLGVTLLASIMPAIKATRVPPV
                     AALQQEAHAAPTHHTWRRVVTGTVVTVIGVALLLTGLFASTGNRLINIASGAVIIFLG
                     VGFLSPLVARPLARLLGWPFAHWAGEPGKLARENAMRSPRRTASTAAALMIGLALVSF
                     VTIFAASLKTSSNKILDESVAADYILSGPSNSGVGFSPQVVQRISQQPEIESATGIRF
                     GAFKLNGKTSQLQAIDPATFPRTVRTDMVSGNLSNLASGDVAVRKDVAKSNSWKVGDT
                     VTMDFPVGGSQQERIAAIYEDNHLNGSYLLTLADYQNHYPDQLDQLGLVKAKPNVSTD
                     ASRAAVERVTNDFPIVQVKDQAQFKRDQAKSINQILLLFYLLLALAVIVAFIGIINTL
                     ALSVLERIRELGLLRAVGMTRAQLRRMIRWEAVIVAVLGAVLGLVVGGFFGWTMVRAL
                     HNQGITEFTLPVGSLLAFVIAAGLAGMAAAIFPGRRAAKVDVLRAITTE"
     gene            complement(8122..8910)
                     /locus_tag="VF995_02870"
     CDS             complement(8122..8910)
                     /locus_tag="VF995_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018846864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342545.1"
                     /translation="MSTSVTPPRETTDQLAARAVDVVKVYGQGETAVRALDGVTVDFP
                     TGRFTAIMGPSGSGKSTLMHCLAGLDRVTSGNIYIGDTDLSGLSEKQLTILRRQRVGF
                     VFQAFNLLPTLTAGENIELPMAIAGRKPDRSWVDRVIDAVGLRDRLGHKPAELSGGQQ
                     QRVAGARALASKPDIVFADEPTGNLDSVASAELLGFMRRSVDEFGQTIVMVTHDPTTA
                     SYSDLVLFLNDGHIVDEMPNPTAEKVLDFLKKLGADMKRVEGEL"
     gene            complement(9163..9549)
                     /locus_tag="VF995_02875"
     CDS             complement(9163..9549)
                     /locus_tag="VF995_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX9342546.1"
                     /translation="MFVGVGDDRRSRVAAALLEHRAKGRVQAVSMSPATVRPDPVVAR
                     SLIGLGIDLRGQRSMPVSQELLERATTVVLMGYTADELAVRTEDWYIDDPSGKDPQTV
                     RYLLDAIDRRVQRLLARLEVGRNLLS"
     gene            9866..11215
                     /locus_tag="VF995_02880"
     CDS             9866..11215
                     /locus_tag="VF995_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX9342547.1"
                     /translation="MQYNSGDTAWVLGSAALVLFMTPGLAFFYAGMVRTKNVLGMLMQ
                     NYVVMGLVSLIWVLVSYSLAFGVDLGGHGIIGTLRFAGLAHMTQPVPGYSGPIAQTIP
                     PLAFVVFQMMFAIITPALITGSTADRLKFGGFVAVIALWAVLVYAPVAHWVFSPTGWL
                     FKLGAEDFAGGTVVHINAGIAGVALAILVGRRRGWPKEPMPPHALPLTLLGAGVLWFG
                     WFGFNAGSALAANQLAAQAFLNTNTATAAALLGWIATEKLRNGRSTTLGAASGAVAGL
                     VAITPACGFVNVVGATVIGLAAGAICALATSVKYRFGLDDSLDVGAVHLVGGVTGALL
                     IGFFGTSTVGGADGLLYGGGLGLLGHQAVAVVVVAVFSFAVTFAIGKLVDVTIGLRVD
                     EAAEREGLDTSQHAETAYELGLVLARGLGVSSATRDHPAPGPPTPAAARNPALGADS"
     gene            11212..11550
                     /locus_tag="VF995_02885"
     CDS             11212..11550
                     /locus_tag="VF995_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX9342548.1"
                     /translation="MRLVTAIVKPFKLGEVKDALSEIGVAGLTVSEVQGYGRQRGHTE
                     VYRGAEYTIDFVPKVRIEVLVTDEAAPGVIDALVKAARTGQIGDGKVWSVPVDEVVRV
                     RTGERGPDAL"
     gene            11501..>12179
                     /locus_tag="VF995_02890"
     CDS             11501..>12179
                     /locus_tag="VF995_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX9342549.1"
                     /translation="MRSSACGPASEAPTRSSPGPGTGPSQPPASSEPRADAERNPLAG
                     AANRAERLAAAKRWLTGLLGDPGPGIALAVGGAYGRGEPAPGSDLDLLLLHTRKPGDQ
                     RQQREVAALAERLWYPIWDAGVRLDHSVRTPEEALAVADEDLKTALGLLDLRHIAGDA
                     ALTQRLREAAGQRWRNTASRRLPELREACRRRAERHGELAFLLEPDLKEARGALRDGH
                     AVSQVAAT"
BASE COUNT         1702 a         4240 c         4362 g         1875 t
ORIGIN      
        1 tcgacgccat ggacgacgac accctgaccg aattcctcat cggcggggcg accaagcggg
       61 agctgctgga gcgtgccgag gcgccgcctt cgctggtgtt ccacacgatc ggcatggatg
      121 acttcctgct cgcgccgctc cccaaccacc tcttcacgcg cgacgcctcc tgctggatcg
      181 gcgacggcgt ggcgatcaac tccatgcgca agaaggcccg caggcgcgaa accatccatt
      241 acgaggcgat ctaccgctgg cacccgctgt tctccagcca ggacttcggc atctggtcgc
      301 agggcaccga gggcggggtc gccaccgtcg aaggcggcga cgtgctggtc atcggcaacg
      361 gcgcggtcct cgtcggcatg agcgagcgca ccacgccggc gggggtggag cggctcgcgc
      421 gccgcctgtt cgaggccggg gcagcgcgcc gcatcgtggc gctggagctg ccgaaggcgc
      481 acgcgttcat gcacctggac acggtcatga cgatggtgga cggcgacacc ttcacgatgt
      541 acgcgggcct gccgccgctg gccgcgcaca cgatcgaacc gggcgacgcc ccgggcgacg
      601 ccggccccgg ctccggcccc gcgaaggagt tgaaggtcac cgaccacccg ccggagccgg
      661 cggcgatgcg cggcgcgatc gcggcgaccc tcggcctcga cgacgtcaag atcctcaccg
      721 ccacccagga cgtgcgctcg gccgagcggg agcagtggga cgacggctgc aacgtgctgg
      781 cggtggcgcc cggcgtggtc atggcctacg agcgcaacgt caccaccaac acctggctgc
      841 ggcgcaacgg cgtggaggtc atcatcgtcc gcggcggcga gctgggccgg ggccggggcg
      901 gtccgcgctg tatgagctgc ccgatcgagc gggaggcggc gtgaccgtcg agaccgtcaa
      961 gcagcggtcc ggcccgggcc gggagcagcg caatggctgg cggagcctgc tgcgcaccaa
     1021 accggtcgag gagctggtcg gcgagagcgg ccgtggccac gagcaaggcg ccgagctgcg
     1081 ccgctcgatg gggctgctgc agctcatcct gttgagcgtc ggcgccacgc tcggcaccgg
     1141 catcttcgtc atcctcggcg acgccgcccc gctcgccggg ccggcggtgg tgctctcgtt
     1201 cctgctcgcg gcggtcaccg cgctgttctc cgcgctctcc tacgcggagc tggccgggac
     1261 catcccggtc tccgggtcct cctactccta cgcctacgcc acgctcggcg agctggctgc
     1321 ctgggtttcc ggctggtgcc tgatggcgga gtacggggtg tcggtgtcgg cggtcgcggt
     1381 cggctggggc cagtacctca acgagctggg ccgctccacc ctcgggttca gcctgccggc
     1441 ggcgatcgcg aacccgcccg gcgagggtgg ggtggtcaac ctcacggcga tcgtggtggt
     1501 ggcggcggcc gcgctgctgc tcatgcgggg aacctcggag agcgccgccg tcaacacggt
     1561 catgacgctg atcaagctgg ccgtgatggt cttcttctgt gccgttgcct gcaccgcgtt
     1621 ccgggcccac aacctgaggc cgttcgcccc gctcggcttc gcgggcatca gcgcggcggg
     1681 cgcgaccgtg ttcttctcct acatcggctt cgacgcggcc tccaccgccg gcgaggaggc
     1741 ccgcaacccc aagcgcgacc tgcccatggc gatcatcatc tcgctcggca tcgtgacgct
     1801 cgtctacgcg gcggtcgcgc tcgccgcggt cggtgtcacg ccatggaaac agctgggcgg
     1861 gctgcaggcg ccgctcgcca gcgtgctcag cgacgcgacc gggagcacct ggccggcggc
     1921 ggtgctgtcg ttcggcgcgg tgatcgcgat cgccagcgtc gtgctcacgg tgatgtacgg
     1981 gcagacgcgc atcctgttcg ccatggcccg cgacggcctc gtcccccgcg tcttcgaccg
     2041 ggtgagcccc cggacccaga ccccggtggc caacacgctg atcgtggccg ggttcatcgc
     2101 ggccctggcc ggcttcttcc cgctcggtca tctggccgac gccgccagca tcggcacgct
     2161 ggccgccttc gcgatcgtca acttcggcgt gctggtgctg cgccggacca gaccggacgc
     2221 gccgcgcagc ttccgcgtgc cgttctaccc gctgacgccg atcctcgggg tgtgcctctg
     2281 cgcctggctc atggccaagt tcgacagggt gacctgggtc gccttcgtgc tgtggaccgt
     2341 ggccggcctg gccgcctatg ccgcctacgg ctaccgccac tcacggctcg gccaggccgc
     2401 cgctgctgcc gctactccag atgctcgtac gcggggagcg tcaggaactc gatgaagtcg
     2461 tcggcgaggg cgacctgctc gaacagggcc tgggcctcgt cggcgcggct ggcggcgaag
     2521 acctcctcgc cgaaggtctg gcgcagcttg tccagctcct cggcctcgac ctggcggacc
     2581 aggtcggggg tgacctcgcg gccgtcgctg agcttgacgc cgttgtggac ccactgccag
     2641 acctgggagc gggcgatctc ggcggtggcg acgtcctcca tcaggttgga gatcgcggcc
     2701 gcgccgctgc cgcgcagcca tgaggagatg tactggatgg ccacgctgac gtcgttgcgc
     2761 aggccctcct cggtgacgtc gccgggggtg gcgggcacgt tcagcaggtc ggaggcgcgc
     2821 acctggacgt cgtcgcgctt gcgctcgatc tggttggagc gcgtgccgag cacctcgtcg
     2881 aagcattcgg cggcggcttg gaccaggtcg gggtgggcga cccaggtgcc gtcgaagccg
     2941 tcgccggcct cgcggcgctt gtcgtcacgg accttggcca gggcgacgcg gttcacctcg
     3001 gggtcgcggc ggctggggat gaacgcggcc atcccgccca tggcgaaggc ctcgcggtgg
     3061 tggcaggtcc ggaccagcaa ctcggtgtag gcgcgcataa acgggacggt catggtcacc
     3121 tggttgcggt cgggcagggt gaactccggc cgggtgcgga acttcttgat gaccgagaac
     3181 atgtagtccc agcggccggc gttcagcccg gaggaatgct cgcgcagctc gtagaggatc
     3241 tcgtccatct cgaacgcggc ggtgatcgtc tcgatcagca cggtggcgcg gatggtgccg
     3301 cgcgggatgc cgagatgatc ctgcgcgagc acgaacacgt cgttccacag ccgcgcttcc
     3361 aggtggctct ccagcttcgg caggtagaag tacgggccgg tacccttgcc gagcaggcgg
     3421 gtggcgttgt ggaagaagta gaggccgaag tcgaccaggg cgccggcggc cggctggccg
     3481 tcaaccagca ggtgcttctc gggcaggtgc cagccgcgcg ggcgcggcat cagcaccgcg
     3541 acctcgtcgt tcagctggta gtggcggcca tcggggccgt cgaagctgat ggtgcgctcg
     3601 atcgcctcga tcaggttggc ctggccgccg aagaggttgt cccaggtggg ggcgttagcg
     3661 tcctcgaagt cggccatgaa tacccgcgcg ccggagttga gcgcgttgat cagcatcttg
     3721 cggtcggtcg ggccggtgat ctcgacgcgc cggtcggcga ggtccgccgg atagggcgcc
     3781 acccgccagt cgccctccct gatggaggcc gtctccggcg ggaagtcggg cagctcgccg
     3841 gcctcgaagg cggcctgccg ctcgcggcgc ttggccagca gctcctccag ggtcgggttg
     3901 aactcgcgct gcagctcggc gacgaacgcc agcgcctcgt cgctcaggat ctgagggtac
     3961 ttctgctgcg ccggcccgtg gacttcgacg ccgcttgggc gtagggacgc catcagggca
     4021 cctccgttcg tattggccct gccattcggc tctgccgtgg tgcctcattg tgccgcatgg
     4081 aaagtctgct tgacagccgc caggtgtaaa gctaccttgt caatgtaaag cgcgcttgac
     4141 tccacgagac gcagtgtcct tccgccggag gtgatgccat gaacaacagg gtccgcgtcc
     4201 tccgcaccga gcgcgggctg tcccagggcg agctggccga ggcgatgggc gtctcgcgcc
     4261 agaccatcaa ctcgatcgag accgagcgct acacgccgtc actcccgctc gcgatcgcgc
     4321 tggcccacta cttcgccaag ccggtcgagg aggttttcaa tgcgaatggc acccaatcaa
     4381 cgtgagcccc gcggcctcgt cagccgcctg tgcggcgggc ttgggggctg gggcatgttc
     4441 ctgttctcgc tcgcgctcgg cgcggtcatg tggaccgcct cgtggatcgg cggccacccg
     4501 atgctcgggc tggcgatgtt cggcatcatg gcggccttcg gcgccgtgtt cgtcgtcggc
     4561 cggcgtagcg agacgatccg cctgatggcg gcgggccggc acgccgatga gcgctggcgc
     4621 tcgatcgacc tgcgcgccac ggccttcatg ggcatcgtgc tggggctcgc ggtgatcgtc
     4681 gccttcctct gggcgatcgc ccacggccgc gacggccagc cctacgcgcc cctcggagcg
     4741 atcgccggga tctcctatct gatcgcgatc atctacctgc gctggaggtc gtgacctgcc
     4801 cagcgggcag gccgcaaggg cctcacggcg cgagccgctc gatcgtccac ggggcggtcg
     4861 ggcgctcgcg cgtgtagcgg aggcggtcgt ggaggcggtt ctcccggccc tgccagaact
     4921 cgatcacgcc aggggtgagc cggtagccgc cccagaacgg cggcaggggg acctcggcat
     4981 ccgcggcatc gccgccgtag cgggccgcca gcgtctcgta gcgccgctcc agcacgtccc
     5041 ggcccgggat cacgctgctc tgcggtgacg cccaggcacc gagccggctg cccactggcc
     5101 gggtgcggaa gtaggcctcg gactcctcca gcgggacccg ctccacccgc ccctcgacgc
     5161 ggacctggcg atgcaccggg ggcggccacc agaacgtcag cgccgcgagc gggttggcgg
     5221 ccaactcctg gcccttgcgg ctctcatggt tcgtgtagaa cacaaacccc cgctgatcca
     5281 cccctttgag cagcaccatc cgcacggacg gccgccccgc gggcgtcgcg gtcgccagcg
     5341 ccatcgcggt cggctccggc agcgccgcgg ccactgcctc cttgaaccac cgctcgaact
     5401 gcccgatcgg atccggatcc acctcagcga tttccaggcc ggccaatccc gctcctctcc
     5461 ccgcgcgctg ggctcactgt accgagatgc cagagcggcg gccccaggcc tgtgcccgtg
     5521 agccgccgct ccgcctcccc ccggagtttg aacgaccgac aacgcctgtt cgcaacgcct
     5581 attcggtcgt gatcgccctc agcacgtcga ccttggcggc gcggcggccg gggaagatcg
     5641 cggcggccat gccggcgaga cccgcggcga tcacgaaggc cagcagcgag ccaaccggca
     5701 gggtgaactc ggtgatgccc tggttgtgga gggcgcggac catcgtccag ccgaagaacc
     5761 cgccgaccac caggccgagg accgcgccga ggaccgcgac gatcacggcc tcccagcgga
     5821 tcatgcggcg tagctgcgcc cgggtcatgc cgaccgcgcg cagcaggccg agctcgcgga
     5881 tccgctccag caccgacagc gccagggtgt tgatgatgcc gatgaaggcg acgatcacgg
     5941 ccagggcgag cagcaggtag aagagcagca ggatctggtt gatcgacttc gcctggtcgc
     6001 gcttgaactg cgcctggtcc ttcacctgga cgatcgggaa gtcgttggtc acccgctcga
     6061 ccgcggcgcg tgaggcgtcg gtggagacgt tcggcttggc cttgacgagc ccgagctggt
     6121 cgagctggtc gggatagtgg ttctggtagt cggccagggt cagcaggtag ctgccattga
     6181 ggtggttgtc ctcgtagatc gcggcgatcc gctcctgctg gctgccgccg accgggaagt
     6241 ccatggtgac ggtgtcgccg accttccagc tgttggactt ggcgacgtcc ttgcgcacgg
     6301 cgacgtcacc gctggccaag ttcgaaaggt tgccggagac catgtcggtg cgcacggtcc
     6361 tggggaaggt ggccgggtcg atcgcctgga gctggctggt cttgccgttc agcttgaagg
     6421 cgccgaagcg aatgccggtg gcggactcga tctcgggctg ctgcgagatg cgctgcacca
     6481 cctgggggct gaagcccacc ccgctgttgc ttgggcccga gaggatgtag tcggccgcga
     6541 cggattcgtc gaggatcttg ttggaggagg tcttcagcga tgcggcgaag atcgtcacga
     6601 agctgaccag cgcgaggccg atcatcagcg ccgcggcggt ggaggcggtg cggcgcgggc
     6661 tgcgcatggc gttttcgcgc gcgagcttgc ccggctcgcc ggcccagtgc gcgaacggcc
     6721 agccgagcag ccgtgccagc ggccgggcca ccagggggct gaggaagccg acgccgagga
     6781 agatgatcac cgcgccgctg gcgatgttga tcaggcggtt gccggtgctg gcgaacagcc
     6841 cggtcagcag cagtgcgacg ccgatcacgg tgacgaccgt gccggtgacc acgcggcgcc
     6901 aggtgtggtg ggtcggggcg gcgtgcgcct cctgctgcag ggcggccacc ggcggcaccc
     6961 gggtcgcctt gatggccggc atgatcgagg cgagcagggt caccccgagg ccgacgacca
     7021 gcgcgacgat gatcgtgcgc ggcgcgatca ccggggtggt ctgcggcagg tcgaccccga
     7081 gcgccttgaa cgcgttgttg agcccgatgg cgaccaggat cccgaagccg atgccgacca
     7141 ccgagccggc caggccgacg atggccgcct cggccagcac cgatcgcagc acctgccgcc
     7201 gcgaggcgcc caggcagcgc agcagcgcca gctcccgggt gcgctgcgcc aggatgatcg
     7261 agaaggtgtt gacgatgatg aacgagccga cgaacagcgc cacgaaggcg aaggccagca
     7321 gagcgatatt gatgaacttg gtgaacgacc cgaccgtctt ggagtcgtcc tgcgccagct
     7381 gcttaccggt gagcacctgg aacttggggt cgacgacggc cttgacccgg tcgcgcagcg
     7441 tcgccgggga cacgcccgag gcggccacgg catcgacctc gtcgtaggtg ccgacacggt
     7501 tcagcacctg ctgcgcggtc ttcaggtcga agccggccag ggtcgcgccg gcgaggttgt
     7561 cggccgtgcc gaagccgagg atgccgctca cggtgaactc gcgcggcggt ccctggaaca
     7621 ggatcttgac ccggtcgccg accttgaagc cctgcttctt ggcggtggcc gcgtcgatcg
     7681 cgacctggtc cgagctggtc ggccgcgctc ccccacgaag ggtggtcgcg gactgcagct
     7741 gcggcaccga gctgagcgag acgccgaggg tgggcgcgcc gccggtggtg actggcttgc
     7801 cgttgctgcc gatgaactgg gcgtagccgt tgacgctgcc ctcggcggcc gcgacgccct
     7861 ggacgccctt gacctggtcg aggactgagg agggcatcgg cgcgcgggtc tggccgccct
     7921 gggcgttgaa ggtcgcgttg gtccggacgg ccacgtcgac gccctgggtg acctggttga
     7981 agaggttgtc gaaggtcttg ttgatggtgt cggtgagcac gaaggtgccc gccacgaagc
     8041 cgaccccgag cacgatcgcc agggcggtca gggacatgcg cagcttgtgg gccgccagtc
     8101 ccttgagggt ggccttcagc atcagagctc gccctccacc cgcttcatgt cggcaccgag
     8161 cttcttcagg aagtcgagca ccttctcggc ggtcgggttc ggcatctcgt cgacgatgtg
     8221 gccgtcgttg aggaacagca ccaggtcgga gtaggaggct gtggtcgggt cgtgggtgac
     8281 catcacgatc gtctggccaa actcgtccac gctgcggcgc atgaagccga gcagctcggc
     8341 gctggcgacc gagtcgaggt tgccggtcgg ctcgtcggcg aacacgatgt ccggcttgga
     8401 ggccagcgcc cgggcgccgg cgacgcgctg ctgctggccg ccggacagct cggcgggctt
     8461 gtggccgagg cggtcgcgca ggccgaccgc gtcgatcacc cggtcgaccc aggagcggtc
     8521 cggcttgcgg ccggcgatcg ccatgggcag ctcgatgttc tcgccggcgg tgagggtcgg
     8581 gagcaggttg aacgcctgga agacgaagcc gacccgctgg cgccgcaata tggtgagctg
     8641 cttttcggag aggcccgaga ggtcggtgtc gccgatgtag atgttgccgg aggtgacccg
     8701 gtcaaggccg gccaggcagt gcatcagggt cgacttgccg gaaccggacg ggcccatgat
     8761 cgcggtgaaa cggccagtgg ggaagtcgac cgtgacgccg tcgagcgcgc ggacggcggt
     8821 ctcgccctgg ccgtagacct tgacgacgtc gaccgcccgc gccgcgagct ggtcagtggt
     8881 ttcccgcggg ggggtcacgc tggtggacat gctcagccct tctgctcggc agcttttcat
     8941 gcggttgatt ggctttgagt gaggctacgc gatgccagtg acggtggcgt ccaccggaca
     9001 ggggattctc catccacctc gaggaggatc tccacccgat cgcgccccgc tacgatgcgc
     9061 cctggcgctg ccaccgcgct gccgtgccgc tgacaccgct gacaccgcgc tgacgcacga
     9121 actgttcgct tcgcgcggac aatcctagct tgggcgaaga cgtcaagaaa gcaaattgcg
     9181 tccgacctcg aggcgcgcga gcaggcgctg gacgcggcgg tcgatcgcgt cgaggagata
     9241 gcggacggtc tgggggtcct tgccgctcgg gtcgtcgatg taccagtcct cggtacggac
     9301 ggccagctca tcggcggtgt agcccatcag caccaccgtc gtggcgcgtt ccagcagctc
     9361 ctgcgacacg ggcatggagc gctgcccgcg caggtcgatg ccgagcccga tcagggagcg
     9421 cgccaccacc gggtctggcc gcaccgtcgc cgggctcatc gacacggctt ggacgcgccc
     9481 cttggcgcgg tgctccagca gcgcggccgc gaccctgctg cggcggtcgt cgccgactcc
     9541 cacgaacacc accagaggga gtcgctggcc cgggaccaac cgcaggtgtc ccggcttcac
     9601 accgatggcc gcaccgtgct cgccgtgcat gggtcgcgtc gccacccgct gtccacctct
     9661 ctcctgtcgg cccgatccgg gctgggccta cctacaaaga cgccggagac gcccacttgg
     9721 ttccctttgg tttgaggatc gacccgcttg ccccgttcac agggccgtaa cagtggtgta
     9781 gcaacccgta aacagccgcg tagcctaggg gaaacgggca gccctgcatg cttttccagt
     9841 cgcgcaatac ggcaagggga gagctatgca gtacaactcc ggagatacgg cgtgggtgct
     9901 gggcagtgcg gccctggtcc tgttcatgac gccggggctg gcgttcttct acgcggggat
     9961 ggtccgcacc aagaacgtgc tcggcatgct gatgcagaac tacgtggtga tgggcctggt
    10021 cagcctgatc tgggtgctgg tcagctacag cctcgccttc ggggtcgacc tgggcggcca
    10081 cgggatcatc gggacgctgc gcttcgccgg gctggcgcac atgacgcagc cggtgcccgg
    10141 ctacagcggg ccgatcgcgc agacgatccc gccgctggcg ttcgtggtct tccagatgat
    10201 gttcgccatc atcacccccg cgctgatcac cgggtccacc gccgaccggc tcaagttcgg
    10261 cggcttcgtc gcggtgatcg cgctgtgggc ggtgctggtc tacgcgccgg tggcgcactg
    10321 ggtgttctcc cccaccggct ggctgttcaa gctcggcgcc gaggacttcg ccggagggac
    10381 ggtggtgcac atcaacgccg gcatcgccgg ggtggcgctg gccatcctgg tgggccgccg
    10441 ccgcggttgg cccaaggagc cgatgccgcc ccacgcgctc ccgctgaccc tgctcggcgc
    10501 cggggtgctg tggttcggct ggttcggctt caacgccggc tcggcgctgg ccgccaacca
    10561 gctcgccgcg caggcgttcc tcaacaccaa caccgccacc gccgccgcgc tgctcggctg
    10621 gatcgccacc gagaagctcc gcaacggcag gtccaccacc ctcggcgccg cctccggcgc
    10681 ggtcgccggg ctggtcgcga tcaccccggc ctgcggcttc gtcaacgtcg tcggggccac
    10741 cgtgatcggc ttggccgccg gggcgatctg cgcgctggcc accagcgtca agtaccgctt
    10801 cggcctggac gactcgctcg acgtcggcgc cgtccacctg gtcggcgggg tcaccggcgc
    10861 gctgctgatc ggcttcttcg gcacctcgac ggtgggcggg gccgacgggc tcctctacgg
    10921 cggcggtctc ggcctgctcg gccaccaggc ggtggccgtg gtggtggtcg cggtgttctc
    10981 cttcgcggtc accttcgcga tcggcaagct ggtcgacgtc accatcggcc tgcgcgtcga
    11041 cgaagcggcc gagcgcgagg gcctggacac cagccagcac gccgagaccg cctacgagct
    11101 gggcctggtc ctggcccgcg gcctcggcgt cagcagcgcc acccgcgacc acccggcccc
    11161 ggggccgccc acccccgcgg ccgcccgcaa cccagcgctt ggagcggact catgagactc
    11221 gtcaccgcga tcgtcaagcc gttcaagctc ggcgaggtca aggacgccct cagcgagatc
    11281 ggcgtcgccg gcctgaccgt cagcgaggtc cagggctacg gccgccagcg cggccacacc
    11341 gaggtctacc gcggcgccga gtacaccatc gacttcgtcc ccaaggtcag gatcgaggtc
    11401 ctggtcaccg acgaggccgc cccgggagtc atcgacgcgc tggtcaaggc ggcccgcacc
    11461 ggccagatcg gcgacggcaa ggtctggtcg gtcccggtcg atgaggtcgt ccgcgtgcgg
    11521 accggcgagc gaggccccga cgcgctctag cccgggtccc ggcaccggcc ccagccagcc
    11581 cccggccagt tccgagccga gggcggacgc cgaacgcaac ccgctggccg gcgccgcgaa
    11641 ccgggcggag cggctcgccg ccgccaaacg ctggctgacc ggcctgctgg gcgaccccgg
    11701 ccccggcatc gcgctggcgg tgggcggcgc ctacgggcgc ggcgagccgg cacccggcag
    11761 cgacctcgac ctgctgctgc tgcacacccg caagcctggc gaccagcggc agcagcggga
    11821 ggtggccgcg ctggcggagc ggctctggta cccgatctgg gacgccggcg tccgcctcga
    11881 ccacagcgtc cgcaccccgg aggaggcgct cgcggtcgcc gacgaggacc tcaagaccgc
    11941 cctcggcctg ctcgacctgc gccacatcgc cggtgacgcc gcgctcaccc agcggctgcg
    12001 cgaggcggcc gggcagcgct ggcgcaacac cgccagccgc cgcctgccgg agctgcgcga
    12061 ggcgtgccgg cgccgcgccg agcgccacgg cgagctggcc ttcctgctgg agcccgacct
    12121 caaggaggcc cgcggcgcgc tgcgcgacgg ccacgccgtc agccaggtcg cggccacct
//
LOCUS       DASWDC010000087         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1435766, whole genome shotgun sequence.
ACCESSION   DASWDC010000087 DASWDC010000000
VERSION     DASWDC010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435766"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /gene="metG"
                     /locus_tag="VF995_02895"
     CDS             complement(<1..1067)
                     /gene="metG"
                     /locus_tag="VF995_02895"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX9342550.1"
                     /translation="MSGKDTFYITTPIYYINDVPHIGHAYTTVAADVLARWRRAHGEQ
                     VMFLTGTDEHGQKVARAAEAAGTTPKAWADRTVERWKETWAQLDISYDDFIRTTEQRH
                     VTAVQHLWQRLHDQGDVYLDSYEGLYCVDCEQFYSEEELVDGCCPIHGRPVELVREEN
                     YFFRISNYADRLLELYRAHPEFVRPEGRLREVTSFVEQGLQDLSISRSSFDWGVPIPW
                     DPKHVMYVWIDALQNYLTAAGWDADMEQYAHLWPADYHFVGKDILRFHAVTWPAILMA
                     AGLPVPRTVQAHGWLLVGGEKMSKTKLTGIAPHDLVEPFGSDAFRYFFQREVAFGQDG
                     NFSWEAMVGRYNADLANGLGNL"
     gene            complement(1128..2072)
                     /gene="rsmI"
                     /locus_tag="VF995_02900"
     CDS             complement(1128..2072)
                     /gene="rsmI"
                     /locus_tag="VF995_02900"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX9342551.1"
                     /translation="MSDALSDPFWFWCKLCGDMPTISTSAADGSPGTGKLVVCATPIG
                     NLEDASPRLARTLAEADLVACEDTRQTRKLLSHFGVRARTVSYHQANEPRRAAQLVDE
                     IEAGATVALVSDAGMPGVSDPGHDLIGACLARGIAVEVVPGPSAVLTALVVSGLPTDR
                     FCFEGFLPRRGGPRARRLEELAAEPRTMVFFEAPHRALATLDAMAAAFGDDRACALAR
                     ELTKLHEEILRGSLAEVRDRLALEPNGPRGEITLVVGGAPAPPAGEVTSADLAAEVAR
                     QVEGGRNSKEAISAVAHATGTPRRVVYQAVLDARKMRC"
     gene            complement(2081..>2422)
                     /locus_tag="VF995_02905"
     CDS             complement(2081..>2422)
                     /locus_tag="VF995_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342552.1"
                     /translation="VLRPGAPLAIYDYVAAAPIAGGAPLANVFVRPQVLIRRAQRAGL
                     TVLASGRPPEVGPVPAPWLEATAAVEAEIVRRHRRDPWCRTELRERAAFTRLRRSGRI
                     IPWLLLLRRDD"
BASE COUNT          344 a          814 c          924 g          340 t
ORIGIN      
        1 aggttgccga ggccgttggc gaggtcggcg ttgtagcggc cgaccatcgc ctcccaggag
       61 aagttgccgt cctggccgaa ggcgacctcg cgctggaaga agtagcggaa ggcgtcggag
      121 ccgaacggct ccaccaggtc gtggggggcg atgccggtga gcttggtctt gctcatcttc
      181 tcgccgccga ccagcagcca gccgtgcgcc tgcacggtgc ggggcaccgg caggccggcg
      241 gccatcagga tcgccggcca ggtgaccgcg tggaagcgca ggatgtcctt gccgacgaag
      301 tggtagtcgg ccggccagag gtgggcgtac tgctccatgt cggcgtccca gccggcggcg
      361 gtgaggtagt tctgcagcgc gtcgatccac acgtacatga cgtgcttggg gtcccagggg
      421 attggcacgc cccagtcgaa gctcgaccgc gagatcgaca ggtcctggag gccctgctcg
      481 acgaacgagg tcacctcgcg caggcgcccc tcggggcgga cgaactcggg gtgggcgcgg
      541 tacagctcca gcaggcggtc ggcgtagttg gagatgcgga agaagtagtt ctcctcgcgg
      601 accagctcga ccgggcggcc gtggatcggg cagcagccgt cgaccagctc ctcctcggag
      661 tagaactgct cgcagtccac gcagtacagc ccctcgtagc tgtcgaggta gacgtcgccc
      721 tggtcgtgga gccgctgcca gaggtgctgc acggcggtga cgtggcgctg ctcggtggtg
      781 cggatgaagt cgtcgtagga gatgtcgagc tgggcccagg tctccttcca gcgctccacg
      841 gtccggtcgg cccacgcctt gggggtggtc ccggcggcct cggcggcgcg ggcgaccttc
      901 tggccgtgct cgtcggtgcc ggtgaggaac atgacctgct cgccgtgggc ccgccgccag
      961 cgggccagca cgtcggcggc cacggtggtg tacgcgtgcc cgatgtgcgg cacgtcgttg
     1021 atgtagtaaa tcggtgtcgt aatgtagaag gtgtcttttc cgctcaccgt cgctcccgtc
     1081 cggcctgtcc gagccgcaaa tccgaggcta gcagccaaag gcggaggcta gcagcgcatt
     1141 ttgcgggcgt cgagcacggc ctgatagacg acccggcggg gggtcccggt ggcgtgggcg
     1201 acggcgctga tcgcctcctt ggaattgcgg ccgccctcca cctgacgggc cacctcggcg
     1261 gccaggtcgg cgctggtcac ctcgccggct ggaggggcgg gcgcaccccc cacaaccagc
     1321 gtgatctcgc cccgggggcc attcggctcg agggcgagcc ggtcgcggac ctcggcgagg
     1381 gagccgcgca ggatctcctc gtggagcttg gtcagctccc gggcgagcgc gcaggcacgg
     1441 tcgtcgccga aggcggccgc catggcatcg agggtggcca gggcgcggtg gggggcctcg
     1501 aagaacacca tggtgcgggg ttcggcggcc aactcctcca gccggcgggc acgggggccg
     1561 ccgcggcgcg gcaggaaccc ctcgaagcag aaacggtcgg tgggcaggcc gctcaccacc
     1621 agggcggtca gcaccgcgct cggtccgggc acgacttcga ccgcgatgcc ccgcgcgagg
     1681 caggcgccga tcaggtcgtg gcccgggtcg gagacgccgg gcatgcccgc gtcggagacc
     1741 agcgcgacag tcgccccggc ctcgatctcg tcgaccagct gggctgcccg gcgcggctcg
     1801 ttggcctggt ggtagctgac cgtccgggcc cggaccccga agtggctgag gagcttgcgg
     1861 gtctgccgag tgtcctcgca ggcgaccagg tcagcttcgg cgagggtccg cgccagccgg
     1921 ggcgaggcgt cctccaaatt cccgatcggg gtggcgcaga ccaccagctt ccccgtcccg
     1981 ggggagccgt ccgctgcgga cgtggagatg gtcggcatgt cgccgcacag cttgcaccag
     2041 aaccagaacg ggtcgctcaa cgcgtcgctc aacgcgtcgc tcagtcgtcg cggcgcagca
     2101 gcagcagcca ggggatgatc cggcccgagc ggcgcaggcg ggtgaaggcc gcccgttcgc
     2161 gcagctccgt acggcaccac gggtctcggc ggtggcggcg gacgatctcg gcctcgaccg
     2221 cggcggtcgc ctccagccag ggcgccggga ccgggccgac ctccggaggc cggccgctgg
     2281 ccagcacggt cagaccagcg cgctgcgcgc ggcggatgag cacctgcgga cggacgaaga
     2341 cgttggcgag cggagcgccg ccggcgatcg gcgccgccgc gacatagtcg tagatcgcca
     2401 gcggcgcgcc gggccgcagc ac
//
LOCUS       DASWDC010000088         7199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1188009, whole genome shotgun sequence.
ACCESSION   DASWDC010000088 DASWDC010000000
VERSION     DASWDC010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188009"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VF995_02910"
     CDS             <1..1112
                     /locus_tag="VF995_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX9342553.1"
                     /translation="RPRRRKALVASVLVLLLAGGGAGAYAVLSNRWSNSDSTVAVKAV
                     TTAAVTRANLVDTEQDDGSLSYDDQQTLHAGNRGVLTALPDEGATVSRGQSLYRVDDK
                     PVTLFYGNLPLYRTLQDGADDGADVKQLEENLDALGYGDNLTVDDHFSSATTDAVKAW
                     QADRGLDQTGTVDATQVLFLPHAVRVGQHKADVGDSVSGGMAVATISSTTRIATVDLP
                     ADNASIARVRDKVDIELPDGHTVKGTITEIAKVATTASAASGSSGGSASSGSSSSSDP
                     TIAVTIRVDDPRATGDLDQAPVKVDFTKQRKTGVLAVPVTALVALSEGGYAVEVSDGG
                     SATHLVKVTTGLFASGQVEVRGSGLRRGQRVVVPA"
     gene            1109..1837
                     /locus_tag="VF995_02915"
     CDS             1109..1837
                     /locus_tag="VF995_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017241104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342554.1"
                     /translation="MRTNRATVAASATDQAAGPVLELRGVRKVYPGPLEVLHGIDLTI
                     EQGELVAILGPSGSGKSTLLHLMGTLDRPSSGTVEVDGHDVAGMSDRALSALRVRRIG
                     FVFQQFFLLDGMSALENVAGGLLYAGVPAARRRELAAATLTRVGLGHRLKHHPAAMSG
                     GERQRVAIARALVAHPAIMFADEPTGNLDSSAGLEILRLLEELHADGTTIVVITHNQE
                     VAAAMRRQIEVLDGRIRYDSGRAA"
     gene            1834..3108
                     /locus_tag="VF995_02920"
     CDS             1834..3108
                     /locus_tag="VF995_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003957984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9342555.1"
                     /translation="MTATLQPQRPRSGRDAPRQGQALAPSRLLPGDLLRVGSIGLRTR
                     RLRAALSALGIAIGIAAIVGVLGISESSSADLLAQLDALGTNLLSVQPGQTLGGANAK
                     LPEGSEGLVSRIGPVQAVSSVASVSATVLRNDRVDPNATGGISVKAARPNLLDVLRGS
                     VAQGQFLTAASERYPVVVLGSVAAQRLGIITLRPGIAVYLGGKWFTVAGILDSMPLAP
                     DIDRSALIGYPEAKAAFPPSGYHSDNVVAPTTVYARTDPDQVQAVHDVLAATADPQHP
                     EEVQVSQPSNALTARAAAKSAFNSLFLGLGGVALLVGGVGIANVMVISVLERRSEIGL
                     RRALGATKGHVRTQFLAESLLLALIGGSGGVAIGAAATAAYATTRSWQIVIPPSAVAA
                     GLGAAVLIGAVAGLYPAVRAARLAPTEALRSV"
     gene            complement(3173..4069)
                     /locus_tag="VF995_02925"
     CDS             complement(3173..4069)
                     /locus_tag="VF995_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9342556.1"
                     /translation="MSSTSASTSIRRVRERQPPPARAAGPAPRPGVEAGRSGSPGVHA
                     ALSVLELLVAHAPLGLSELAAELRLAKSTLHRICAVLVERGWVVRDREGRFDLGARAI
                     GIGARSGELPVVTGFRGIAARLLTAHNETVCLAVLDGDDSMFIALEETSHPVRLVTTV
                     GNRTPAFAAASGQVVLAERSPAVIEADYGGRLLVTPTGRRLRDVGELQEILAQVRRQG
                     YAESHGETAEGLYSVSAPVRNDAGAVLAAMTLCVPTCRVDPARREALISDLVAASAGL
                     SAEVAWLPAWNATRADRPRTAL"
     gene            4381..6072
                     /locus_tag="VF995_02930"
     CDS             4381..6072
                     /locus_tag="VF995_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9342557.1"
                     /translation="MDGLQRRFDEVRADRTELENHLIDELAAGRLTRRDFVRRGTVLG
                     MSAAVLSFVAAACGKSTTNTNTGGSTGSVKKGGAMTVGIIAPSGAINPLTVADEGGLA
                     VLGQAGEYLTWSDKDLKLSGVLAESWSPNADASVWTFKLRQNVTFNDGTSMKAADVVK
                     TFQAHSDPNGKSNALSAFSGVLSPHGVQAVDDHTVAFHLDAPNGNFPYLVSSDNYNTI
                     VLPASYDYAGKYEQKFAGTGPWKMTSFKTGSEVAYEKNPSYWQASAGFPVLNNLTLKF
                     YDTEQPRILALQSKSLDVVSQFSASTGQQLLSNPSIKVLALRSSAHRQVHMRTDKEPF
                     TDKLVRQAIALLIDRPGLVNGLFNGKAEAGNDSPFAAVFPSTDTSVPQRTQNVDQAKQ
                     LLAAAGKSGGFTVTLATWNGYEIPLLAQAIKAAVKQVGGTINLSITPPGTYYGDAVFG
                     KSPWLDSTMGITDYGHRGVPNVFLGAPLTSKGTWNSAHFHNAQYDGLVKQYVAAVDLS
                     SQRAVAKQIETLLLDETPIIFPYFYSHLTATQSNIANVEPTAMGHIRMLQAGFTS"
     gene            6092..7045
                     /locus_tag="VF995_02935"
     CDS             6092..7045
                     /locus_tag="VF995_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9342558.1"
                     /translation="MARFLLRRFGLALLTLWLLATIVFLGAHVLPGDVGRRILGPFAA
                     PGAVKALNHELGTDQPIPLQYWHWLSSAARGRLGESLSFRVPVSSLLGPALLNSLKLA
                     LVAFVLVVPLGVLGGVVAGLADGRPLDRLVTVGGLSATVVPEFVWSIVVILVFAVKLR
                     LLPVTAQAPLGSGPLTQFHYLILPAFPLVFVLFGYIARITRAGMIEALEADYTRTAVL
                     KGISRRAVIARHVLRNGLLPTIAVVATQTGYLIGGLVVIELIFNYQGIGQLVYLSAQK
                     KDFPLLQGTVLVIGIVYLTVTLLADITYSLLNPRIRYGSAE"
BASE COUNT         1066 a         2698 c         2442 g          993 t
ORIGIN      
        1 ggcgcccacg gcgccgcaag gcgctggtcg ccagcgtgct ggtgctgctg ctcgccgggg
       61 gcggagccgg cgcctacgcc gtgctcagca accgctggtc gaactcggac agcaccgtcg
      121 ccgtcaaggc cgtcaccacc gccgcggtca cccgcgcgaa cctggtcgac accgagcagg
      181 acgacggctc gctcagctac gacgaccagc agacgctcca cgccggcaac cgcggcgtgc
      241 tcaccgccct gcccgacgag ggcgccacgg tcagccgcgg ccagagcctg taccgggtgg
      301 acgacaagcc ggtgacgctg ttctacggca atctccccct gtaccggacg ctccaggacg
      361 gggccgacga cggcgccgac gtgaagcagc tcgaggagaa cctggacgcg ctcggctacg
      421 gcgacaacct caccgtcgac gatcatttct cgtcggcgac caccgacgcc gtcaaggcct
      481 ggcaggccga ccgcgggctg gaccagaccg gcacggtcga cgccacccag gtcctgttcc
      541 tgccccacgc cgtccgtgtc ggccagcaca aggccgacgt cggcgacagc gtcagcggcg
      601 ggatggcggt ggccaccatc tcctccacca cccgcatcgc caccgtcgac ctgccagccg
      661 acaacgccag catcgcccgc gtccgcgaca aggtcgacat cgagctgccg gacggccaca
      721 ccgtcaaggg caccatcacc gagatcgcca aggtcgccac caccgccagc gcggcctcgg
      781 gcagctctgg cggctcggcc tcaagcggct cctccagctc cagcgacccg acgatcgcgg
      841 tcaccatccg ggtcgacgat ccccgcgcga ctggcgacct cgaccaggcg ccggtgaagg
      901 tggacttcac caagcagcgc aagacgggcg tgctggcggt gccggtcacc gcactggtcg
      961 cgctgtcgga gggcggctac gcggtcgagg tcagcgacgg cggctctgcc acgcacctgg
     1021 tcaaggtcac caccggcctg ttcgccagcg ggcaggtcga ggtgcgcggc agcggcctgc
     1081 ggcggggcca gcgggtggtg gtgccggcat gaggaccaac cgcgcaaccg tcgccgcctc
     1141 ggcgacggac caggcggccg ggccggtgct ggagctccgc ggcgtccgca aggtctatcc
     1201 gggcccgctc gaggtgctgc acggcatcga cctgaccatc gagcagggcg agctggtcgc
     1261 gatcctgggc ccgtcgggct ctggcaagtc gaccctgctg cacctgatgg gcaccctcga
     1321 ccggccgtcg tccggcaccg tcgaggtcga cggccacgac gtcgccggca tgagcgaccg
     1381 cgcgctgtcg gcgctgcggg tgcggcggat cggcttcgtg ttccagcagt tcttcctgct
     1441 ggatgggatg agcgcgctgg agaacgtcgc cggtggcctg ctctacgccg gcgtgccggc
     1501 ggcccgccgc cgggagctgg cagccgcgac gctcacccgg gtcggcctcg gccaccgcct
     1561 caagcaccat ccggcggcca tgtccggcgg ggagcgccag cgggtcgcga tcgccagggc
     1621 cctcgtcgcg cacccggcga tcatgttcgc cgacgagccg accggcaacc tcgactcctc
     1681 ggccggcctg gagatcctcc ggctcctcga ggaactgcac gccgatggca ccaccatcgt
     1741 cgtgatcacc cacaaccagg aggtggccgc ggccatgcgg cgccagatcg aagtgctcga
     1801 cggccgcatc cgctacgact ccgggagggc ggcatgaccg cgacgctgca accccagcgg
     1861 ccgcggtccg gccgcgacgc gccgaggcag gggcaggcgc tggcgccgag ccgcctgctg
     1921 cccggcgacc tgctgcgggt cggctcgatc gggctgcgca cgcgccggct gcgggccgcg
     1981 ctgtcggcgc tcggcatcgc catcggcatc gcggcgatcg tcggcgtgct cggcatctcc
     2041 gagtcgagca gcgccgacct gctggcgcag ctcgacgccc tcggcaccaa tctgttgtcg
     2101 gtgcagccgg gccagaccct cggcggcgcc aacgccaagc tccccgaggg gtccgagggg
     2161 ctggtcagcc ggatcggccc ggtccaggcg gtgtcgtcgg tggccagcgt gtcggcgacg
     2221 gtgctccgca acgaccgggt ggaccccaac gccaccgggg gcatcagcgt caaggcggcc
     2281 aggcccaacc tgctcgacgt gctgcgcggc agcgtcgccc aagggcagtt cctcaccgcc
     2341 gccagcgagc gctaccccgt ggtcgtgctc ggctcggtgg cggcgcagcg gctcggcatc
     2401 atcaccctgc ggccggggat cgccgtgtac ctcggcggca agtggttcac ggtggccggg
     2461 atcctcgaca gcatgccgct ggcgcccgac atcgaccgct cggcgctgat cggctacccc
     2521 gaggcgaagg cggcgttccc gccgtcgggc taccactccg acaacgtcgt cgcgcccacg
     2581 accgtctatg cccgcaccga tcccgaccag gtccaggcgg tccacgacgt gctcgccgcc
     2641 accgccgacc cccagcaccc cgaggaggtg caggtcagcc agccctccaa cgcgctgacc
     2701 gcccgcgcgg cggccaagag cgccttcaac tcgctgttcc tcggcctggg cggcgtcgcg
     2761 ctgctggtcg gcggagtcgg catcgccaac gtcatggtca tctcggtgct ggagcgccgc
     2821 tccgagatcg gcctgcggcg cgcgctcggc gccaccaagg ggcacgtgcg cacccagttc
     2881 ctggccgagt cgctgctgct cgccctgatc ggcggcagcg gcggggtggc gatcggcgcg
     2941 gcggcgaccg ccgcctacgc caccacccgg agctggcaga tcgtgatccc cccgagcgcg
     3001 gtcgcggccg gcctgggcgc cgccgtgctc atcggcgcgg tcgcggggct gtacccggcc
     3061 gtccgggccg cccgtctggc accgaccgag gccctgcgct cagtctgagc gctgggccgc
     3121 atgccgggga ggaggccgcc gcctcctccc ccaaccctgt cacgagggtc gactacaggg
     3181 ccgtgcgggg gcggtcggcg cgggtcgcgt tccaggcggg cagccaagcg acctccgccg
     3241 acagccccgc gctggccgcc accaggtcgc tgatcagcgc ctcccggcgg gccgggtcaa
     3301 ctcggcaggt ggggacgcac agggtcatcg cggccagcac cgccccggcg tcgttgcgga
     3361 ccggggcgga gacgctgtag aggccctcgg cggtctcgcc gtggctctcg gcgtagccct
     3421 ggcgacgcac ctgggcgagg atctcctgca actcgccgac gtcgcgcagc cgccgcccgg
     3481 tgggggtgac cagcagccgg ccgccgtaat cggcctcgat caccgcgggg gagcgctcgg
     3541 cgaggaccac ctggccgctg gcggcggcga acgccggggt gcggttgccg acagtggtga
     3601 ccagccgaac cgggtgggag gtctcctcca gggcgatgaa catcgagtcg tcgccgtcga
     3661 gcaccgccag gcagaccgtc tcgttgtggg cggtgagcag ccgcgcggcg atcccccgga
     3721 agccggtgac caccggcagc tcccccgacc gcgcaccgat cccgatggcg cgggcgccca
     3781 ggtcgaagcg cccctcccgg tcacgcacca cccagccccg ctccaccagc accgcgcaga
     3841 tccgatgcag ggtgctcttg gccagacgca gctcggcggc cagctccgag aggccgagcg
     3901 gcgcgtgtgc caccagcagc tccagcacac tgagagccgc gtggaccccg ggcgacccgg
     3961 agcgtccggc ctccaccccc ggccgcgggg ccggcccagc cgcccgggcg gggggcggct
     4021 ggcgctcgcg cacccgcctg atcgaagttg acgctgaggt actggacatc ctggtgctcc
     4081 tcaagcccga gccgcccatc ctcgcggccg ccgccggact gtttgatccc gccgaagggg
     4141 ggcctgaacc cgggctgccg cgacgctatg ttccatcggt ggaccaggct cctttcagct
     4201 gaacgcatgg tcagcccggc gaaagggcct gtcaaggcca caaccagccg gatatggata
     4261 cttgacagca caggacgtac atgcaaaaag tttccgagta tccattggca gaacgcatat
     4321 ccgcagaagg ttcgcaaagc aggttcgcgg ccgtgagctc cgggattccg ggggtgggac
     4381 atggacgggt tgcagcgccg tttcgacgag gttcgggccg accggaccga gctggagaac
     4441 cacctgatcg acgaactggc cgcgggccgg ctgacgcggc gtgacttcgt ccgccgcggc
     4501 accgtcctcg ggatgtccgc ggcggtgctg agcttcgtcg ccgcggcctg cgggaagtcc
     4561 acgaccaaca ccaatacggg cggcagcacc gggtcggtca agaagggcgg cgcgatgacc
     4621 gtggggatca tcgccccctc cggcgcgatc aaccccctga ccgtcgccga cgagggcggc
     4681 ctggccgtgc tcggccaggc cggcgagtac ctcacctggt cggacaagga cctgaagctc
     4741 agcggcgtgc tggccgagtc atggtcgccc aacgccgacg cctcggtgtg gaccttcaag
     4801 ctgcgccaga acgtcacctt caacgacggc acctcgatga aggccgccga cgtggtcaag
     4861 accttccagg cccactccga ccccaacggc aagtcgaacg cgctgtccgc gttctccggg
     4921 gtgctgagcc cccacggcgt ccaggcggtc gacgaccaca ccgtcgcctt ccacctcgac
     4981 gcccccaacg ggaacttccc gtacctggtc agctccgaca actacaacac catcgtcctg
     5041 ccggccagct acgactacgc cggcaagtac gagcagaagt tcgccggcac cggcccgtgg
     5101 aagatgacca gcttcaagac cggctccgag gtcgcctacg agaagaaccc gagctactgg
     5161 caggcctcgg ccgggttccc ggtgctcaac aacctcaccc tcaagttcta cgacaccgag
     5221 cagccgcgga tcctggcgct gcagtccaag agcctcgacg tggtcagcca gttctccgcc
     5281 tccaccggcc agcagctgct cagcaacccc tccatcaagg tgctggcgct gcgctcgtcc
     5341 gcgcaccggc aggtgcacat gcgcaccgac aaggagccct tcaccgacaa gctggtccgg
     5401 caggcgatcg ccctgctgat cgaccgcccc ggcctcgtca acgggctctt caacggcaag
     5461 gccgaagccg gcaacgactc gccgttcgcc gcggtgttcc cctccaccga cacctcggtg
     5521 ccgcagcgca cccagaacgt cgaccaggcc aagcagctcc tcgccgccgc cggcaagtca
     5581 ggcggcttca ccgtgacgct ggcgacctgg aacggctacg agatccccct gctggcgcag
     5641 gcgatcaagg cggcggtcaa gcaggtcggc ggcaccatca acttgagcat caccccgccg
     5701 gggacctact acggcgacgc ggtgttcggc aagtcgccct ggctcgactc gaccatgggg
     5761 atcaccgact acggccaccg cggcgtcccg aacgtgttcc tcggtgcccc gctcacttcc
     5821 aaggggacct ggaactctgc ccacttccac aacgcccagt acgacgggct ggtcaagcag
     5881 tacgtggccg cggtcgacct gagctcgcag cgcgccgtcg ccaagcagat cgagaccctg
     5941 ctgctggacg agaccccgat cattttcccg tacttctact cgcacctgac cgccacccaa
     6001 tccaacatcg ccaacgtgga gcccaccgcc atggggcaca tccgcatgct ccaggcaggg
     6061 ttcacgtcgt agcgggggcg ggcggggcat cgtggcccgg ttcctgctac gccgcttcgg
     6121 cctggcgctg ctgacgctgt ggctgctcgc caccatcgtc ttcctcggcg cgcacgtgct
     6181 cccgggtgac gtcggccgcc gcatcctcgg cccgttcgcc gcgccggggg cggtcaaggc
     6241 gctgaaccac gaactcggca ccgaccagcc gatcccgctg cagtactggc actggctgtc
     6301 gagcgcggcg cgggggcggc tcggcgagtc gctctcgttc cgggtgccgg tgtcgtcgct
     6361 gctcgggccg gcgctgctca actcgctgaa gctcgcgctg gtggcgttcg tgctggtggt
     6421 gccgctgggc gtcctcggcg gggtggtggc ggggctcgcc gacggccgcc cgctcgaccg
     6481 gctggtgacc gtgggtggcc tgtcggccac ggtggtcccc gagttcgtct ggagcatcgt
     6541 ggtgatcctg gtgttcgcgg tgaagctgcg gctgctgccg gtcaccgccc aggcgccgct
     6601 cggcagcggg ccgctcaccc agttccacta cctgatcctg cccgcgttcc cactggtgtt
     6661 cgtgctgttc ggctacatcg cccggatcac ccgcgcgggc atgatcgagg ccctcgaagc
     6721 cgactacacc cgcaccgcgg tgctcaaggg catctcacga cgggcggtga tcgcccgcca
     6781 cgtgctccgc aacggcctgc tcccgacgat cgcggtggtg gccacccaga ccggctacct
     6841 gatcggcggg ctggtggtga tcgagctgat cttcaactac cagggcatcg gccagctggt
     6901 ctacctctcc gcccagaaga aggacttccc cctgctgcag ggcaccgtcc tggtcatcgg
     6961 catcgtctac ctgacggtga ccctgctggc cgacatcacc tactcgctgc tcaacccacg
     7021 gatccgctac ggaagcgcgg agtgaacgtg agccagcgat gagacaccct ttgtcaacca
     7081 cgggttcagg cggcgcggcc gagggcgcgg ttgcggttgg cgacgtggat ggcctcgtcg
     7141 tacagctcgc ccttgcgcag gcagtgccac aggacttcca gccagcggtt gccgagcgc
//
LOCUS       DASWDC010000089         8976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1436095, whole genome shotgun sequence.
ACCESSION   DASWDC010000089 DASWDC010000000
VERSION     DASWDC010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8976
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436095"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="VF995_02940"
     CDS             complement(<1..1397)
                     /locus_tag="VF995_02940"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX9342559.1"
                     /translation="MNVLEATGVVKAYGGVVALRGCDFAVAPGTVHALLGENGAGKST
                     LVKILTGAVRPDAGVVALDGKPVRFSDTADAARNGVAVVSQELNLFPDLDVLANLFTK
                     RELLRGVLFDRRRMTAEAEPVLAELGLDVSPRTPVSDLSLAQRQLVEVGKALLARPRV
                     LILDEPTSALDDSGVRRLLGVIEVLRQRQVAVVFVSHILEESMQLSDVVTVLRDGQVV
                     MAAAQRRSLTIDDIVHSMLGDKLVAAEERTAALVRHRVGRSNNGSVPKAEAEPAAAGG
                     GPGQGTTADALVCQDLTVPEVLDGVSLTAHVGEIVGLAGLAGAGHHALLEVVAGQLAA
                     SQGTIRLPRGRAPDGGRDGGRDGGDQRPRRPPRSLRQAVRHGIAVVSGDRRAGLMLDK
                     PIWHNVAQVRAVAQLGDGLVLRPQRLRRRAWDRITQLGVRTSSVDREAGLLSGGNQQK
                     VVLAKWLEAPPPPCCS"
     gene            complement(1439..2551)
                     /locus_tag="VF995_02945"
     CDS             complement(1439..2551)
                     /locus_tag="VF995_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX9342560.1"
                     /translation="MKSSIVGDRLGLWRMPAVAVVAALLFAATACSSKSNNASGGGGG
                     GGSCATASKKLNLAFVYASTTDNPFQEMALGAKAAADEDGNVKLTETAPTGIDGPKEV
                     TLFQSAARSATDGVAYMTTFPNLFVRPLQSALSANVPIVAVDALAPKGADVKTAVANS
                     NTALGVALGEAFVKANPNPNGEVVEGLDIPTLPLLQQRMAGFTSVVKQKLPNMKISGP
                     FDTKAEATDNYNAWKAVVQAHPNATAFVGVGGQDGVSLPLIEQQTGKHFLAGSADLPP
                     QALTAVKKGQIFALSSPEHWMKGYIAMRMLIQSKRNCTAIPEGWWNSGNLIVDKSNID
                     DIIARQQTADSRKAWFQKVVSEQLTSKPIQPLSAAN"
     gene            complement(2623..3693)
                     /locus_tag="VF995_02950"
     CDS             complement(2623..3693)
                     /locus_tag="VF995_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9342561.1"
                     /translation="MATDGTAREGQVVTAASPEPEAPPPPVGRRLREGIGRVVRAPSA
                     SVVAATVLLVLLIGALRPNFLQASQMFDILQGSTYVGLLAGGMAYLIAMREIDLSVGS
                     MFGLCLIAVGLLIRSGMNPWLAALAGIGLGGVLGAVNATIVAWIRIPTIVATLATLSV
                     YRGLTFGITAGDQVVNLPVEKSFFTVLGGKVGGVPMAVIVLALVALLVGLALHQTPFG
                     YRVLAIGSNPEAAAFSGISVTRVRTQVLILTGVLAGIAGVLGLAFFTSGDPNIGSGFE
                     LQAIAAAVIGGTPLRGGSATILGAAFGAILLNVVASGLSYFSIPANWSAFATGLVILL
                     AVGVDSFVRSRRQARKGAGAGL"
     gene            3886..4764
                     /locus_tag="VF995_02955"
     CDS             3886..4764
                     /locus_tag="VF995_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9342562.1"
                     /translation="MEERRAVGRPFRAVYDDASRSVLARGLRLLDAFGSGDTTLTLTE
                     LAERTGLPKPTAYRLLSQLVAWGGLERDGCAYRIGMKLFVLGQRVPLQRRLRDAALPY
                     LEDLYEATHENIHLSVLDGAGTLFLEKVSGRDSMPIGSRVGVRMPAYCTATGKVLLAF
                     GPAEQLHRVVDAGLVRHTPRTIVLPDLLRLDLARTLERGYGINREEAEVGVSAVAAPV
                     FDHHQRVIAAISITGYAHRLDHERLAPAVVTAARSLSRELSREAVNEASLRFTPLPPA
                     PAPQPLAPSDHRLTAS"
     gene            4859..5374
                     /locus_tag="VF995_02960"
                     /pseudo
     CDS             4859..5374
                     /locus_tag="VF995_02960"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
     gene            5523..6698
                     /locus_tag="VF995_02965"
     CDS             5523..6698
                     /locus_tag="VF995_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX9342563.1"
                     /translation="MRPHGRGARPAKHHLDRHGRHHVPGRPGAGAARPGPACYGGGGD
                     EPTVTDVDVVLGIINPEHFLGGRKRLDPELAAEAIRRRVAEPLGLPVGDAAAAIYAIQ
                     NGQTADLVRKVVVNSGQDPRDFVLYSYGSYGGAGPVHCASYAADLGVPEVIVPLGATA
                     AVFSAYGLTASDIVLTAELSQPANFPVPPDEISEVFRGLEKDLRARLAEQQLDFAAVA
                     YEREADLRYTMQLAEVPTPVPPGSTSAGLPWTASPRSLACSAGGLAEADVAEIGRQFE
                     ISYEALYGKGSGFSDAGLQVITYRLRATGQLPTRPRLPRLAAASTDRPAPTGTRQVFL
                     DVRHGWRTTSIYDYRDLAAGHRIEGPAVVEAPTTTVALPEGCSARVDTLGNLVLQLA"
     gene            6942..7550
                     /locus_tag="VF995_02970"
                     /pseudo
     CDS             6942..7550
                     /locus_tag="VF995_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
     gene            7657..>8976
                     /locus_tag="VF995_02975"
     CDS             7657..>8976
                     /locus_tag="VF995_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX9342564.1"
                     /translation="MTKQGDTLRFDCTGTDPQADGFINCTLAGLRGGIIPIVLTMLCG
                     DIPWAPGGVFRCIEVISEPGTLNHCTFPAGIGKASVASAWATQNAVSECVAAMLATSA
                     EHDTSLMAVCCGTWDLALIAGIDQRGASFVTLLCDPMAGGLGARVDRDGVDTGGVNNI
                     PMGRCADVEINEFSFPILYLWRREEPDSGGPGRFRGGLGGSSCFVPHDSPTGGVHLVV
                     SAPGKAIPQAPGISGGYPANTQYDVLVRDAGVRERLAAGHIPAALDELGGQAQVVPPH
                     LETDMAAGDVYFTHWQGGGGYGDPLLRDPALVAADVRDRKVSPFAADLVYGVALRPDG
                     TADAEATAAHRSRLRRERAGLAADREESEADGEAESEADGEEAERLKIDDNVVVAADG
                     TTACAHCSATLGTAAREPLERALRRERPATDAGPGVHADPALFTDRPV"
BASE COUNT         1271 a         3218 c         3222 g         1265 t
ORIGIN      
        1 gagcagcagg gtggaggggg tgcctccagc cacttggcga gcacgacctt ctgctggttg
       61 cccccggaga gcaggccggc ctcccggtcg acgctggagg tcctgacgcc gagctgggtg
      121 atgcgatccc aggcgcgcct gcggagccgc tgggggcgca ggacgaggcc atcgccgagc
      181 tgggcgaccg cacggacctg ggcgacgttg tgccagatgg gcttgtcgag catgagcccg
      241 gccctgcggt cgccggagac caccgcgatg ccgtggcgca cggcctggcg gaggctccgg
      301 ggtggccgcc gggggcgctg gtcgccgccg tcgcggccac cgtcgcggcc accgtccggg
      361 gcccggccac ggggcagccg gatggtcccc tgggaggcgg ccagctggcc ggcgacgacc
      421 tccagcaggg cgtggtgccc ggcacccgcc aggccggcga gacccacgat ctcgccgacg
      481 tgggcggtga ggctgacgcc gtcgagcacc tccgggacgg tcaggtcctg gcagacgagg
      541 gcgtcggcgg tcgtgccctg gccggggcca ccgcccgcgg ccgcgggctc ggcttcggcc
      601 ttggggacgc tgccgttgtt gctccggccg acgcggtggc ggaccagcgc cgcggtgcgc
      661 tcctcggcgg cgaccagctt gtcgccgagc atgctgtgca cgatgtcgtc gatcgtcaga
      721 ctgcggcgct gcgcggcggc catcaccacc tggccgtcgc gcagcacggt gaccacgtcg
      781 ctgagctgca tcgactcttc gaggatgtgg gagacgaaca ccaccgccac ctggcgctgg
      841 cgcagcacct cgatgacgcc gagcagccgc cgcacgccgc tgtcgtccag cgccgaggtg
      901 ggctcgtcga ggatcaggac gcgcggccgg gcgagcagcg ccttgccgac ctcgacgagc
      961 tggcgctgcg ccaggctcag gtcgctgacg ggcgtgcgcg gcgacacgtc gaggccgagc
     1021 tcggcgagca ccggctcggc ctccgcggtc atgcggcggc ggtcgaacag cacgccgcgg
     1081 agcagctccc gcttggtgaa gaggttcgcc agcacgtcga ggtcggggaa gaggttcagc
     1141 tcctgggaga ccaccgcgac gccgttgcgt gccgcgtcgg cggtgtcgga gaagcgcacc
     1201 ggcttgccgt ccagggcgac cacgccggcg tcgggccgca ccgcgccggt gaggatcttg
     1261 accagggtgg acttgccggc gccgttctcc cccagcaggg cgtgaacggt gccaggggcg
     1321 acggcgaagt cgcagccccg cagggcgacg accccgccgt aggctttgac gaccccggtg
     1381 gcctcgagca cgttcacgcc cactgcgctc cctccggtgg ggtgcttcga ttcgccgctc
     1441 agttggcggc ggacagcggc tggatcggct tggaggtgag ctgctcgctg acgaccttct
     1501 ggaaccacgc cttgcggctg tccgcagtct gctggcgggc gatgatgtcg tcgatattgg
     1561 acttgtcgac gatcaggttg cccgagttcc accagccttc ggggatcgcc gtgcagttgc
     1621 gcttggactg gatgagcatg cgcatcgcga tgtagccctt catccagtgc tccggcgagg
     1681 acagcgcgaa gatctgcccc ttcttgacgg cggtgagggc ctgcggcggc aggtccgccg
     1741 agccggcgag gaagtgcttc ccggtctgct gctcgatcag cggcagcgac acgccgtcct
     1801 ggccgcccac gccgacgaac gcggtcgcgt tcgggtgcgc ctgcacgacc gccttccagg
     1861 cgttgtagtt gtcggtcgcc tcggccttgg tgtcgaacgg gccgctgatc ttcatgttcg
     1921 gcagcttctg cttgaccacg ctggtgaacc cggccatgcg ctgctgcagc agcggcagcg
     1981 tcgggatgtc gaggccttcc acgacctcgc cgttcgggtt ggggttggcc ttgacgaagg
     2041 cctcgccgag cgccacgccg agcgcggtgt tcgagttggc gaccgcggtc ttgacgtcag
     2101 cgcccttcgg cgcgagcgcg tccacggcga cgatcgggac gttcgcgctc agcgcgctct
     2161 gcagcggccg cacgaacagg ttggggaagg tggtcatgta ggccacgccg tcggtggcgc
     2221 tgcgggccgc cgactggaag agggtgacct ccttgggccc gtcgatgccg gtgggcgccg
     2281 tctccgtcag cttgacgttg ccgtcctcgt cggcggctgc cttggcgccg agcgccatct
     2341 cctggaaggg gttgtcagtg gtggaggcgt agacgaacgc caggttgagc ttcttgctgg
     2401 cggtggcgca ggagcctccg ccgcccccgc caccactggc attgttcgac ttcgagctgc
     2461 aagcggtggc ggcgaacagc agggcggcca cgacggccac cgccggcatg cgccagaggc
     2521 cgagccgatc acccacgatg gacgacttca tgcctgtctc cttgccttgg acggaagagg
     2581 gctcccgatc acctgtcaca tacgtgccga tcacatacga cctcacaaac cggccccggc
     2641 acccttcctt gcctggcggc ggctgcggac gaagctgtcg acgccgaccg cgagcaggat
     2701 caccaggccc gtggcgaacg cgctccagtt ggccgggatg ctgaagtagg acaacccgct
     2761 cgcgaccacg ttgagcagga tcgcgccgaa cgcggccccg aggatcgtcg cgctgccccc
     2821 tcgtaggggg gtcccgccga tcaccgccgc ggcgatggcc tggagttcga agcccgagcc
     2881 gatgttgggg tcgccggagg tgaagaacgc cagcccgagg acgcccgcga tgcctgccag
     2941 cacgccggtc aggatgagga cctgggtgcg cacgcgggtg acggagatgc cggagaacgc
     3001 cgcggcctct gggttggagc cgatggcgag cacccggtag ccgaacggtg tctggtgcag
     3061 cgccaagccg accagcagcg cgaccagcgc caggacgatc acggccatcg ggacgccgcc
     3121 gaccttgcct ccgagcacgg tgaagaacga cttctcgacc ggcaggttga cgacctggtc
     3181 gccggccgtg atgccgaacg tcaggccgcg gtagaccgac agcgtcgcca gggtcgccac
     3241 gatcgtcggg atccggatcc aggcgacgat cgtggcgttg accgcgccga ggacgccgcc
     3301 gaggccgatc cccgccagcg cggccagcca gggattcatg ccagagcgga tcagcagccc
     3361 cacggcgatg aggcagaggc cgaacatcga tcccaccgac aggtcgatct cgcgcatcgc
     3421 gatgaggtag gccatgccgc cggcgagcag cccgacgtag gtcgaaccct gcaggatgtc
     3481 gaacatctgg ctggcctgca ggaagttggg ccgcagcgcg ccgatgagga gcaccagcag
     3541 caccgtggcc gccacgacgc tcgccgaggg cgcccgcacc accctgccga tcccctctcg
     3601 gaggcgcctg cccaccggcg gcggcggcgc ctcgggttcg ggagacgccg cggtgacgac
     3661 ctggccctct ctggccgtgc catcagtcgc cacgaatgcg ctcactcctt tcttgaaccg
     3721 ccgcttcttg aagagccgct gcgctggtgt gggtgaaggc tcttctggcg cgctcgcggt
     3781 cacaacaggt ctgtttcagt gaatgaaatc gctgtgccgc gggtgcgggg ggccgcgtgc
     3841 ctgcagccgt ttgcacaggt tgtgacgtgg cgagatgatc aaccgatgga agagcggcga
     3901 gcggtgggga ggcccttccg ggccgtatac gatgacgcct ccagatcggt cctcgcccgg
     3961 gggctgcggc tgctggacgc cttcggcagc ggcgacacca cgctgaccct caccgagctg
     4021 gccgagcgca ccggcctgcc gaagccgacc gcctaccggc tgctcagcca gctggtcgcc
     4081 tggggcggcc tggagcgcga tggctgcgcc taccgcatcg gcatgaagct cttcgtgctc
     4141 ggccagcggg tccccctgca gcgccgcctg cgcgacgccg cgctgcccta ccttgaggac
     4201 ctctacgagg ccacccacga gaacatccac ctgtccgtgc tcgacggcgc cggcacgctg
     4261 ttcctggaga aggtgagcgg gcgcgactcg atgccgatcg gctcccgggt cggggtgcgc
     4321 atgccggcct actgcaccgc gaccggcaag gtgctgctgg cgttcggccc ggcagagcag
     4381 ctccaccgcg tggtggacgc cgggctggtg cgccacacgc cgaggacgat cgtgctgccc
     4441 gacctgctgc gcctggatct cgccaggacg ctggagcggg gctacggcat caaccgggag
     4501 gaggcggagg ttggtgtctc ggcggtggcg gctccggtgt tcgaccacca ccagcgggtg
     4561 atcgcggcca tctcgatcac cggctacgcc caccgcctcg accacgaacg cctcgccccc
     4621 gcggtggtca cggcggcccg gtccctgtcc cgcgagctgt cccgcgaggc cgtcaacgag
     4681 gcgtcgctcc ggttcacccc gctgcctccc gcaccggcgc cccagccgct cgcgccgtcc
     4741 gaccaccggc tcaccgcgtc ctgacgctcg caaccgcacg gtcggctccc agagcatcgc
     4801 gttatcagtt ataaccatcc ggtctgatgg cagatatgat ctcagatatg aggtggctat
     4861 ggcctacgtc atcgggatcg acaccggcgg gaccttcacc gacgccttcg ccgccgatca
     4921 ggacgggcgc atcgcctcgg cgaaggtgcc gtcgacgccg ccggacttcg cccggggctt
     4981 cctcgccgtc atcgacgagc tggcggccac cctcgggatc agcaccggcg cgttgctggc
     5041 ggagaccgcc tacctcaccc acggcaccac ctcgacgctg aacgccctgc tcaccgggga
     5101 ggtggccagc gtcggcctga tcaccaccca ggggcacgcc gactccgtcg cgatcatgaa
     5161 cgtcgagggc cgctacgccg gcctcggccc cgacgaggtc cagaacatgc cccgcaccag
     5221 caagccgccc ccgctggtgc cgcgccggcg gatccgggag gtccacgaac gggtggacta
     5281 caagggcgaa gtggtggtcc cactcgacga ggccggggcc cgagacgcgg tcgccgccct
     5341 gctcgccgac ggggtggacg cgatcgccgt gtcgctcttg tggtcgttcc gcaatccggt
     5401 gcacgagctg cgcgtccgcg agctggtccg cgaggccgcc ccggacctgt acgtggcgct
     5461 gtccagcgag gtcagccccc gcatccgcga atacgcgcgc actgccacca cgatgtggtc
     5521 ggctgcggcc acatggccga ggcgctcggc cagcgaaaca tcatctcgac cgacatgggc
     5581 ggcaccacgt tcctggtcgg cctggtgcgg gagcggcgcg gcccggcccc gcctgctacg
     5641 gcggaggcgg cgacgagccg accgtcaccg acgtcgacgt ggtcctcggg atcatcaacc
     5701 ccgagcactt cctcggcggg cgcaagcgcc tggacccgga gctggccgcc gaggcgatcc
     5761 gacgccgggt cgccgagccg ctggggctgc cggtcggcga cgcggccgcc gcgatctacg
     5821 cgatccagaa cggccagacc gccgacctgg tccgcaaggt ggtggtcaac agcggccagg
     5881 acccgcgcga cttcgtcctc tactcctacg gctcctacgg cggcgccggg ccggtgcact
     5941 gcgccagcta cgcggcggac ctcggcgtcc ccgaggtgat cgtgccgctg ggcgcgaccg
     6001 cggcggtgtt ctcggcctac gggctgaccg catcggatat cgtcctcacc gctgagctgt
     6061 cgcagccggc caacttcccg gtgccgcccg acgagatcag cgaggtcttc cgcggcctgg
     6121 agaaggacct gcgggcacgc ctggccgaac agcagctcga cttcgccgcc gtggcctacg
     6181 agcgcgaggc ggacctgcgc tacaccatgc agctggcaga ggtcccgacc ccggtcccgc
     6241 ccggctccac ctccgccggc ctcccatgga cggcatcgcc tcgctcgctt gcatgctcgg
     6301 ccggcgggct ggccgaggcg gacgtcgccg agatcggccg gcagttcgag atcagctacg
     6361 aagccctcta cggcaaggga tcggggttca gcgacgccgg cctgcaggtg atcacctacc
     6421 gcctgcgcgc caccgggcag ctccccaccc gcccgcgact gccgcggctc gcggcggcga
     6481 gcaccgaccg gcccgccccg accggcaccc ggcaggtgtt cctggacgtc cgccacggct
     6541 ggcggacgac ttcgatctac gactaccgcg acctggccgc cggccaccgg atcgagggac
     6601 cggcggtggt cgaggccccc acgaccacgg tggcgctgcc ggagggctgc tcggcccggg
     6661 tggacacgct cggcaacctg gtcctccaac tcgcctgatg actgacgaca tggcgcgacc
     6721 ctgaacgcct gaaggaaggt gtggcacaga tgccggtgat ccccgtcgcg gggtcggaga
     6781 agttcgactg ccagccgcgg ccctccgacg agctgcgcga gctgctcgcc tcctccggga
     6841 gcggcttgtc gctgcacacc gtcgacgccg acgccgtcga agcgctggac ccgctcacct
     6901 acgaggtggt gcggcaccgc ctggtggcga tcaccgggga gatgggcgcg gcgctcaagc
     6961 gcatgtcggg ctcggtggtg gtcactgact gcaacgactt cggcgccgcc atcctcgacg
     7021 agggcggcga catggtccag gtcggcctct acaacatgca gctggccgcc tcgctggaca
     7081 tggcggtcaa gtggaccctg gagcaccgct cgcggaaccc cgggatccgg ccaggggaca
     7141 tgttcctgtg caacgatccc tgggtcggcg gcggcctcca ccagaacgac gtcagcgtgc
     7201 tggccccgct gttccacgat ggcgagctgt tcgcctggac cggggcggtg gcacatcagg
     7261 tcgacctcgg cggggtctca ccggggagct ggtcggttgg ggggctggac gtgttctggg
     7321 agtcgacccc ggtcccgccg gtcaggatcg tggaggatgg cgtgctgcgc caggacgtcg
     7381 aggacgtcta cctgcgccgc tcgcgggtgc cgaagctgat cgcgctggac ctccgcgcca
     7441 agatcggcgc caactcggtg gcgcaggacc gcctcaggag gctgatcgag aagtacgggc
     7501 cggacacggt caaggctgtg atgaagcggc tgatggacga cgcggagacg cgcctgcgcg
     7561 ccaagctgcg ttcgctgccg gacggcacct ggagcgccgt cggccaccag gacgccgcgc
     7621 gcgggcgacc gcggcctcta caaggtcgtc gtgacgatga ccaagcaggg cgacaccctg
     7681 cggttcgact gcaccggcac cgacccgcag gccgacggct tcatcaactg caccctggcc
     7741 gggctgcgcg gcgggatcat cccgatcgtg ctgacgatgc tctgcggcga catcccctgg
     7801 gcgccgggag gggtcttccg ctgcatcgag gtgatcagcg agccgggcac cctcaaccac
     7861 tgcaccttcc cggccggcat cggcaaggcg tcggtggcct cggcgtgggc gacccagaac
     7921 gcggtctcgg agtgcgtggc ggcgatgctg gccaccagcg ccgagcacga cacctctctg
     7981 atggcggtct gctgcgggac ctgggacctg gcgctgatcg ccgggatcga ccagcgcggc
     8041 gccagcttcg tcacgctgct ctgcgacccg atggccggcg gcctcggcgc ccgggtggac
     8101 cgcgacggcg tcgacaccgg cggggtcaac aacatcccga tgggccgctg cgccgacgtg
     8161 gagatcaacg agttcagctt cccgatcctc tatctgtggc ggcgcgagga acccgactcc
     8221 ggcgggcccg gccgcttccg gggcgggctc ggcgggtcga gctgcttcgt cccgcacgac
     8281 tcgccgaccg gcggggtcca cctggtggtg tcggcgccgg gcaaggcgat cccgcaggct
     8341 ccggggatca gcggcggcta tcccgccaac acccagtacg acgtgctggt ccgcgacgcc
     8401 ggcgtgcgcg agcggctcgc cgccgggcac attcccgccg ccctcgacga gctgggcggc
     8461 caggcgcagg tggtgccgcc gcacctggag accgacatgg ccgccggcga cgtctacttc
     8521 acccactggc agggaggcgg cggctacggc gacccgctgc tgcgcgaccc ggcgctggtc
     8581 gccgccgacg tccgcgaccg caaggtgtcg ccgttcgcgg cggatctcgt ctacggcgtg
     8641 gccctgcgcc cggacgggac cgccgacgcc gaggccaccg cggcccaccg gtcccggctg
     8701 cgccgcgaac gcgccggcct cgccgccgat cgtgaggagt ccgaggccga cggcgaagcc
     8761 gagtccgagg ccgatggcga ggaggccgag cgcctgaaga tcgacgacaa cgtggtggtc
     8821 gccgccgacg ggaccaccgc ctgcgcgcac tgctcggcga cgctcgggac cgccgcgcgg
     8881 gagccgctgg agcgggccct gcgccgcgag cggcccgcca ccgacgccgg accgggcgtc
     8941 cacgccgacc cggccctgtt caccgaccgt ccggtg
//
LOCUS       DASWDC010000090        14971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_594842, whole genome shotgun sequence.
ACCESSION   DASWDC010000090 DASWDC010000000
VERSION     DASWDC010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14971
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594842"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VF995_02980"
     CDS             <1..904
                     /locus_tag="VF995_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9342565.1"
                     /translation="TVGEPIEPDVWRWYHDVVGKGEAVIVDTWWQTENGGFLGSTLPA
                     LLPMKPGSCGPGVLGVYPVIYDEEGNEVEAGSGRAGNICIRNPWPGIFQTIWGQPERF
                     VSTYYEKYNRDKDSKDWRDWPYFAGDGAVKAADGYFRILGRVDDVINVAGHRLGTKEL
                     ESASITVNEVAEAAAVPVIDDLRGRAVEMYIALKPGVQASNDVAGKVTKAIETEIGKI
                     ARPKNVWIVPDMPKTRSGKIMRRVIASISNFADVGDITTLANPEIVDSIRHQVQSEKV
                     SRGETPRELTPQELEEIKRFGQSE"
     gene            complement(972..1997)
                     /locus_tag="VF995_02985"
     CDS             complement(972..1997)
                     /locus_tag="VF995_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9342566.1"
                     /translation="MFWLEIGPGDLAASRFAISPIHEAVVAVRALAGIESGVDATVRQ
                     WLEPKRAQFERLRRNAPMVGVLVELLSASTYTPTFLAPPPSRSRGALESQLEAVRATP
                     LPLARAEIAACLSGSARPLPLPVRAVLAEPDIAIRLADALEAAWNELIRPDWPVIHTI
                     LQQDQQHWARRLLSQGWPGVLRHMPMGRLSWRASGSTQTLHGPDIKEDLVVEHCEPGS
                     GGIVFGPTLAAGMSAVLDPGWQREIRYPALGRATLFQPSQRSQPASDPLGKLIGHTRA
                     RLLCGLDQPTTTTQLARLHRLSLGTVAHHLSAMRDAGLITCIRDGPSVIYYRTLLGDA
                     LVDANLL"
     gene            2385..2458
                     /locus_tag="VF995_02990"
     tRNA            2385..2458
                     /locus_tag="VF995_02990"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2419..2421,aa:Arg,seq:cct)
     gene            2471..3013
                     /locus_tag="VF995_02995"
     CDS             2471..3013
                     /locus_tag="VF995_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX9342567.1"
                     /translation="MGKLLYSVTMSLDGFIAGPGGDMSWLTEHLGPNPAVDVLIGQIG
                     ALLVGNRTFRGDDPHKGTGKEGKPFGGGWSGPQFVLTHDPPDTPVEGVTFVGDLGSAI
                     AAAKAAAGDRYVNVLGADTARQCLDAGALDEILVLIAPVLLGDGVRLFDRPGGANVKL
                     ERISLTQAPQATNLWFRVAR"
     gene            complement(3050..3205)
                     /locus_tag="VF995_03000"
     CDS             complement(3050..3205)
                     /locus_tag="VF995_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobic protein"
                     /protein_id="HEX9342568.1"
                     /translation="MVWIALLIVLILFGFGFAVKALLWIALILLVLWLLGWLIRPGAT
                     TRRWYYW"
     gene            3482..6994
                     /locus_tag="VF995_03005"
     CDS             3482..6994
                     /locus_tag="VF995_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX9342569.1"
                     /translation="MKRGAMTDGDPVADDRFLDELLQAINAAKAGDQTVRLPARRDGL
                     TATVARAFNELVERNPAKEMARVISAVAEGDLTQKMALDFDGRPVEGEFKRIGTALNS
                     MVDQLRAFSAEVTRVAREVGTEGRLGGQAEVPGVAGTWKDLTDSVNGMASNLTDQVRN
                     IAQVTTAVASGDLSRKITVEARGEVAELKDTINTMVDQLGSFADEVTRVAREVGTEGR
                     LGGQAYVKGVSGTWKDLTDNVNQLAANLTAQVRNIAQVTTAVASGDLSRTITVEALGE
                     VAELKDTINTMVDRLSAFADEVTRVAREVGTEGKLGGQAYVKGVSGTWKDLTDSVNSM
                     ADNLTDQVRNIAQVTTAVASGDLSQKITVDVRGEILLLKNTINTMVDQLGSFADEVTR
                     VAREVGTEGKLGGQAEVEGVSGTWKRLTENVNQLAANLTTQVRAIAEVATAVTEGDLT
                     STITVDAPGEVGQLKDNINQMIANLRETTTRNEDQDWLNSNLARIGNMLQGHRDLRTL
                     SRLLMSDLTPLVGAQYGAFFIAGDGDNDKDKDKDRDAAEPRYKLLASYGFRHRRGVTT
                     SFRAGEGLVGQVVVDKRPIVVEDAPTGYIRISSGLGEGAPVNIVVLPVVFEERVLAVI
                     ELASFQRFSAVRRAFLEQLVETVGVVLNTILATMRTEELLKQSQSLAEELQLQQEELR
                     KQQDELQHTNDELQEKAMQLARQNRAIEVKNAEIELARRELEERAEQLALSSRYKSEF
                     LANMSHELRTPLNSLMILAKLLAENQSGNLTEKQVEFAQSMYEAGNDLLKLISDILDL
                     SKVEAGKMDISIAPVDLVTLANDIEQMFRPIAEQQALDFAVEVAPDTPDPVVTDERRV
                     QQVLKNLLSNAFKFTEEGSVRLRIGPPPAGTRFTTPGLRAAPEVIAFSVVDSGIGIAS
                     DQLLVIFEAFQQADGTTNRRYGGTGLGLSISREIARLLGGELHVESEPARGSVFTLLL
                     PAVAEPREAGPEDESAAEPAGDAAADPAGSGAAVAAAVAAATRRGEVAVDGPASVPGS
                     DAARAAGLGLSAAETMLAGKCVLIVDDDVRNVYALTSVLENRGIEVLVAEDGRTGIEL
                     LKRRPAVDVVLMDIMMPVMDGYETTRAIRQLDGFRDLPIIALTAKAMRGDREKCLAAG
                     ASDYITKPVDTDQLLDMLRVWLYR"
     gene            7153..8331
                     /locus_tag="VF995_03010"
     CDS             7153..8331
                     /locus_tag="VF995_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9342570.1"
                     /translation="MDDKPGNLLALTAVLEQLDVDLVGVGSGEEALRRLLVEEFALIV
                     LDVQMPGLDGFETARLIKQREKTRYIPIIFLTAISGAPEHYLRGYGLGAVDYVYKPFA
                     PDILRAKVGVFLELWQRGTLIERQRAELERRLAELDRAHETLARQANDLERSNAALQR
                     FAEVAAHELQAPLHNAAGFLDLLLARHRDALDEEATLLAERAARGVTEMRALTGRLLD
                     YAKAATDTLATEQVALPEVLEAAERDLAEPLGAAGAVVLAERLPTVPGDRRMLICLLV
                     NLLDNAVKFRGEAAPLVRVSAEAAPGLWRVTVRDNGVGVDPDELPRLFTVFARLHPTA
                     DRPGHGIGLATCRRIVERHGGVIWCEPVPPEEGGGALFRFVLPAGAEWPPAGDAEEPR
                     "
     gene            8331..9122
                     /locus_tag="VF995_03015"
     CDS             8331..9122
                     /locus_tag="VF995_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9342571.1"
                     /translation="MPRVLIVDDDPDYRLLVRLALATDPRFEVVGEAEDGNLAAAAAT
                     LCEADLALLDCSMPGADAFDALPAIRRARPSCRVVLLSGHATEDLRAAAWSSGVLGYL
                     GKDVRPSRLGEELVAVIGMVDAVESVLKETSTQLAHDPRSPRVARAFVRRTMEGWRLQ
                     RPLDTAVLLVSELVTNAVLHAGTDVRVILQLTEHTARVEVADQASAPPPLLRRDHDPA
                     SPSGRGLRMVSSLARRWGTYHESAGGKTVWFELARDGGPIGTGLG"
     gene            9164..10477
                     /locus_tag="VF995_03020"
     CDS             9164..10477
                     /locus_tag="VF995_03020"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX9342572.1"
                     /translation="MQRRAIERLEGERDRLERLLLRLPSLMGELDVGKLAQGVVEAAV
                     ELSGARFGAFAAADSDEETVVFVGATGPPPAFAETPALKAAPLLAGAIWSGEAVRVDD
                     VTAWASRDESARAYGVFADGRLVRSWLAVPVRGRGGAVLGAIYLGHHRAHAFSGRQHE
                     LVGGLCSQLGVAMENAELFAERGRIVATLQKSLLPPLLPDIPGVDLAARYRPTGATNL
                     VGGDFFDVFEMADDRTWGLILGDVSGFGAEAAALTGIARYTVRAIAGPGDKPSDVLAQ
                     LNEAMLHQGPAERFCTALYALATLPSAAEGTGGGVEVRLANGGHPPGLVLRDDESVER
                     LEEGAGILLGVFSDPVFADEQVTLRPGDALVLYTDGVIEARDLTGTQFGEDRLAAVLA
                     TCAGRSADGIARRVELAALDHQGNQPSDDIAIVVLRARPTAHPAS"
     gene            complement(10418..10693)
                     /locus_tag="VF995_03025"
     CDS             complement(10418..10693)
                     /locus_tag="VF995_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342573.1"
                     /translation="MRKHPELSQASQEERMVYKIGGLCFAVLGAVLGVAGLLHGGQAV
                     VSLGFGAVTFLLLGGVCYLTGRDPVREPLGSRVGGGAGAQDHDGDVV"
     gene            10870..12180
                     /locus_tag="VF995_03030"
     CDS             10870..12180
                     /locus_tag="VF995_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX9342574.1"
                     /translation="MHRTRLPATLLAAALIAMGLLVAASRPVAAAATVLYVDQSNASC
                     SDSGAGTQTAPFCTIGQGAAVATAGQTVQVASGTYNELVTVAHSGTSGAPIVLTAAPG
                     ASVIVSGKADAFKIASKSYVTVRNFTVTGTSSYGVLVANSSNIVISGNHVSAAGQRVS
                     GSTKYGIKFVGSTNALVSGNVSHDNSDAGIFLDATTNGVEVTGNTTYGNARGYTRAAP
                     GIDVRGHHNTVDRNVSHNNEDSGLQFYSGSHDNLVVDNLSYRNGDHGIDDLHSTGQRI
                     LSNTVFGNVTAGVNLEGSSTGATVENNISVDNGVNSPRTKSNIRVDATSTSGTTVNWN
                     LVDLSVSGQVMYVWGAKSYATLSAFQSASGQESHGRQANPSWVAAGSNFHLKAGSPAI
                     DSANSGVSGETSTDLEGKARRDDSATANTGTGPRTYDDRGAYEF"
     gene            complement(12168..14774)
                     /locus_tag="VF995_03035"
     CDS             complement(12168..14774)
                     /locus_tag="VF995_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX9342575.1"
                     /translation="MVERSDGDARRERRPLHLIGNSHIDPVWLWRWPEGYQAVLATFR
                     SVLERMGEYPEFVFTCDSVAFLAWVEEHDPELFGEIRRRVAEGRFEIAGGWWVEADCN
                     LPCGESLVRQALYGQRWLLDKLGITATVGCNLDPFGHPATLPQLLRKSGLDSYLFLRP
                     GPGELPLAGAGFWWQSADGSRVLAFRVPHEYQTPGIDLAEHLRAALAQLAEVGADHEV
                     ACCYGVGNHGGGPTRANLDSIRRLGGTDGFPPLECSRAREFFDRLQAADGDHLPVVAG
                     ELQPHAVGCYAAHSGIKRQNRRVEHLLLAAEKWAVGAAVLSEGACPYPAAALTHAWKQ
                     LLFNQAHDVLAGTAVAAAYDDARDQLGEAAAIASRALYGAAQAVAGRVGIPEEPGTTP
                     LVAFNPHPWPVRAAIEFEVARGSALLVTDDGGDPVPSQATRSHATVPWRERVVFAAAL
                     PALGWVLFRLRSKALDSALQSWFANDIMSRGGGVVDPPANGPRTPSLDILEGEHLLLR
                     VDAATGWLASLRDKASGVELLPPESSPHAVVIDDGSDTWGHGVRSFTGVGDPEDDPQV
                     VGTFTCRSVRLVEHGPVRSVLRVESEYGHSTLVEEFLLGREARHLEVRVTVDWRERLK
                     LLKLRFPTALEAVTATAEVPCGQVVRATDGEERPAQAWVDVSGHLPGAGRRAGLALLN
                     DGKYSFDVGGGTIGMTVLRSPVSAWHDPEPLRPGVPYEYLDQGVQRFTYRLVPHAGDW
                     RAAGVVRLAAELNQQPFALLESAHPGSLAPRGSLATVTPAAVVVTALKRAEGEGPAAP
                     GDPGGDLIVRAYESAGRPAEATIDLPLVGRTVRARFSPGEIKTFRVPLDPALPARETD
                     LLEFPLELPQNS"
     gene            complement(14783..>14971)
                     /locus_tag="VF995_03040"
     CDS             complement(14783..>14971)
                     /locus_tag="VF995_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342576.1"
                     /translation="RVVGDAFVSAVHVGYLLSAAAGLICATLAVTLLGAFRRQPAPST
                     PAGTEAAAPAPDEPLRRP"
BASE COUNT         2342 a         5390 c         5251 g         1988 t
ORIGIN      
        1 gaccgtcggc gagcccatcg agccggatgt gtggcgctgg taccacgacg tcgtcggcaa
       61 gggcgaggcc gtgatcgtcg acacctggtg gcagaccgag aacggcggct tcctcggcag
      121 caccctgccg gccctgctgc caatgaagcc cggcagttgc ggccccgggg tcctcggcgt
      181 ctacccggtg atctacgacg aggaaggcaa cgaggtcgaa gccggcagcg gccgggccgg
      241 caacatctgc atccgcaacc cctggcccgg catcttccag accatctggg ggcagcccga
      301 gcggttcgtg tcgacctact acgagaagta caaccgcgac aaggacagca aggactggcg
      361 cgactggccg tacttcgccg gcgacggcgc ggtcaaggcg gccgacggct acttccgcat
      421 cctcggccgg gtcgacgacg tgatcaacgt cgccggccac cgcctcggca ccaaggagct
      481 ggagtcggcc agcatcaccg tcaacgaggt cgccgaggcc gccgcggtcc cggtgatcga
      541 cgacctccgc ggccgcgcgg tggagatgta catcgccctc aagcccggcg tgcaggcgag
      601 caacgacgtc gccggcaagg tcaccaaggc gatcgagacc gaaatcggca agatcgcccg
      661 gccgaagaac gtctggatcg tgccggacat gcccaagacc cgctccggca agatcatgcg
      721 ccgggtgatc gcctcgatct ccaacttcgc cgacgtcggc gacatcacca ccctcgccaa
      781 ccccgagatc gtcgacagca tccgccacca ggtccagagc gagaaggtct cccggggcga
      841 gaccccccgc gagctgaccc cgcaggaact ggaggagatc aaacgcttcg gccagtccga
      901 ataatcgata atcggcgaac cagtcacaaa gggggccgcg ttcgtgggcg cggcttcctt
      961 tgttcccgtt gctacaggag gttcgcgtcg acgagcgcgt caccaaggag ggtgcggtag
     1021 tagatgacgg acggcccgtc gcggatgcag gtgatcagcc ccgcgtcgcg catcgcggac
     1081 aggtgatggg cgacggtgcc gaggctgaga cgatggagac gggcgagctg cgtggtcgtg
     1141 gtcggctggt cgagtccgca cagcaggcgg gcgcgggtgt ggccgatcag cttgcccaac
     1201 gggtcgctgg ccggctggga ccgctgggac ggctggaaga gggtggcccg ccccagcgcg
     1261 gggtagcgga tctcgcgctg ccagcctgga tcgagcacgg ccgacattcc cgcggccagg
     1321 gttgggccga agacgatccc gccgctgccg ggctcgcagt gctcgacaac caggtcttcc
     1381 ttgatgtcgg gtccgtgcag cgtctgggtg ctgccgctgg cgcgccagga gaggcggccc
     1441 attggcatgt gccgcagcac gcccggccag ccctggctga gcagccgccg ggcccagtgc
     1501 tgctgatcct gttgcaggat ggtgtggatg accggccagt ccgggcggat cagctcgttc
     1561 caggcggcct ccagggcgtc ggccaggcgg atcgcgatat cgggttctgc cagcacggcc
     1621 cgcaccggca gggggagcgg ccgagcgctg ccggacaggc aggccgcgat ctcggcgcga
     1681 gccagtggca gcggcgtggc ccgcacggct tcgagctggg attcgagcgc gccgcgggag
     1741 cggctgggcg gtggagcgag gaaggtcggc gtatacgtgc tggcgctcag gagctcgacc
     1801 aggacgccga ccatcggcgc gttgcggcgc aggcgctcga actgcgcgcg tttcggttcc
     1861 agccattgcc tgacagtggc gtcgaccccc gactcgatgc ccgccaaggc ccggaccgcg
     1921 accacggcct catgtattgg ggaaatggca aaccggcttg ccgccaggtc accaggtccg
     1981 atctccagcc agaacatgtt acgagcgtaa tcgaaacgtt ggcgcaggaa cgccagagcg
     2041 ggcagagtcg ctttcggcca cgccggtccg gtggctccgt gggcagagcg gttcaaccag
     2101 ccgcacctcc tgatctgccc atcgtcatcg gcggcggcaa gcggctgttg cgcccgacgc
     2161 caacaggtcc accccctcgc gcttgccgcc gccgagacct ccagcaagtc agaggcgccc
     2221 ggcggcgatg atccgcttca ggaaggtctg cgtgcgcggg tgcgccgggg cggtgaagat
     2281 ctgctccgga tcgccctgct cgcaaatcac cccgtcgtcc aggaagcaga cccggctgga
     2341 gacctccctg gaagcgtcaa tgacggcggt agactcagcc tcgcgcccgc gtagctcagt
     2401 cggatagagc agccgcctcc taagcggcag gccgcgcgtt cgagtcgcgc cgcgggcacg
     2461 gaggtgatcc gtggggaagc tgctgtattc ggtcaccatg tcgctggacg ggttcatcgc
     2521 cgggccgggt ggtgacatgt cgtggttgac cgagcatctc gggcccaatc cggcggtcga
     2581 cgtgctgatc ggccagatcg gcgccctgct ggtcgggaac cggaccttcc ggggcgacga
     2641 tccccacaag ggcacgggca aggagggcaa gccgtttggc ggggggtgga gcgggccgca
     2701 gttcgtgctc acccacgacc cgcctgacac gccagtggag ggggtcacct tcgtcgggga
     2761 tctcggcagc gcgatcgcgg cggccaaggc tgcggcgggt gatcgctacg tcaacgtgct
     2821 cggcgccgac accgcgcggc agtgcctcga cgcgggtgcg ctcgacgaga tcctggtgct
     2881 gatcgcgccc gtcctgctcg gcgacggcgt ccgtctgttc gaccgcccgg gcggcgccaa
     2941 cgtcaagctc gaacgcatca gcctgacgca ggcgccgcag gccacgaatc tgtggttccg
     3001 cgtcgcccgc tgagccggtg atccggccgc cggccgcggt gatcattggt taccagtagt
     3061 accagcggcg ggtggtggcg ccagggcgga tcagccagcc gaggagccag agcaccagca
     3121 ggatcagggc gatccacagg agtgctttga cggcgaagcc gaatccgaac aggatcagga
     3181 cgatgagcag cgcgatccaa accatgtgcc tcccctcctt ccgttttgat gtggtccgaa
     3241 gggtctctgc tcgaatatga gcgttggagt ggcccgccaa accaacccgc caaaccagcc
     3301 ggtcagacca gggacggcgc gctcatccgg tatggcgagc gtggcctatg ccttgactga
     3361 ctcgtccgga tagaatcgcc gcctcagaag ctggcaattt gctccagtgc cgccagggcg
     3421 ccctgctccc gcgttcgtgc gacgccgtga tgcccgggac gggaccatta tgtagtttga
     3481 gatgaagaga ggcgccatga ccgacggcga cccagtggcc gacgaccgct tcctcgacga
     3541 gctcctccaa gcgatcaacg ccgccaaggc cggcgaccag acggtccgcc tgccggcacg
     3601 ccgggacggc ctgaccgcga cggtggcgcg cgccttcaac gagctggtgg agcgcaaccc
     3661 cgccaaggag atggcccggg tcatctcggc ggtggccgag ggcgacctca cccagaagat
     3721 ggccctcgac ttcgacggac ggccggtgga gggcgagttc aagcgcatcg gcaccgccct
     3781 caactcgatg gtcgaccagc tgcgcgcctt ctcggccgag gtcacccggg tggcgcgcga
     3841 ggtcggcacc gaaggccgcc tcggcggcca ggcggaggtc cccggcgtgg cggggacctg
     3901 gaaggacctg accgactcgg tcaacggcat ggcatccaac ctgaccgacc aggtccgcaa
     3961 catcgcccag gtcaccaccg cggtcgcctc cggcgacctg tcccgcaaga tcaccgtcga
     4021 ggcgcgcggc gaggtcgccg agctgaagga caccatcaac accatggtcg accagctcgg
     4081 gagcttcgcc gacgaggtca cccgcgtcgc ccgcgaggtc ggcaccgaag gccgcctcgg
     4141 cggccaggcc tacgtcaagg gcgtctccgg cacctggaag gacctcaccg acaacgtcaa
     4201 ccagctcgcc gccaacctga ccgcccaggt ccgcaacatc gcccaggtca ccaccgcggt
     4261 cgcctccggc gacctgtccc gcaccatcac cgtcgaggcc cttggcgagg tcgccgagct
     4321 gaaggacacc atcaacacca tggtcgaccg gctctcggcc ttcgccgacg aggtcacccg
     4381 cgtcgcccgc gaggtcggca ccgaaggcaa gctcggcggc caggcctacg tcaagggcgt
     4441 ctccggcacc tggaaggacc tcaccgactc ggtcaactcg atggccgaca acctcaccga
     4501 ccaggtccgc aacatcgccc aggtgaccac ggcggtggcc tccggcgacc tgtcgcagaa
     4561 gatcaccgtc gacgtccgcg gcgagatcct gctgctgaag aacaccatca acaccatggt
     4621 cgaccagctc ggctccttcg ccgacgaggt cacccgcgtc gcccgcgagg tcggcaccga
     4681 aggcaagctc ggcggccagg ccgaggtcga gggggtctcc ggcacctgga agcggctgac
     4741 cgagaacgtc aaccagctgg ccgccaacct caccacccag gtgcgggcga tcgccgaggt
     4801 ggccacggcg gtgaccgagg gcgacctgac cagcacgatc acggtggacg cgcccggcga
     4861 ggtcgggcag ctgaaggaca acatcaacca gatgatcgcc aacctgcgcg agaccaccac
     4921 gcgcaacgag gatcaggact ggctcaactc caacctcgcc cgcatcggca acatgctgca
     4981 gggccaccgt gacctgcgca ccctgtcgcg gctgctgatg tcggacctga cgccgctcgt
     5041 gggcgcgcag tacggggcct tcttcatcgc cggggacggc gacaacgaca aggacaagga
     5101 caaggacagg gacgccgccg agccgcgcta taagctgctg gcgagctacg ggttccgtca
     5161 ccgccgcggc gtcaccacca gcttccgcgc cggcgagggc ctggtcggcc aggtggtggt
     5221 ggacaagcgg ccgatcgtgg tcgaggacgc ccccaccggc tacatccgca tcagctccgg
     5281 cctgggcgag ggcgcgccgg tgaacatcgt ggtgctgccg gtggtcttcg aggagcgcgt
     5341 gctggcggtc atcgagctgg cctcgttcca gcgcttcagc gcggtgcggc gggccttcct
     5401 ggagcagctg gtcgagaccg tcggggtcgt gctcaacacg atcctggcca cgatgcgcac
     5461 cgaggagctg ctgaagcagt cccagagcct cgccgaggag ctgcagctcc agcaggagga
     5521 gctgcgcaag cagcaggacg agctgcagca caccaacgac gagctgcagg agaaggcgat
     5581 gcagctggcc cgccagaacc gggccatcga ggtcaagaac gccgagatcg agctggcccg
     5641 ccgggagctg gaggagcgcg ccgagcagct ggcgctcagc tcgcgctaca agtcggagtt
     5701 cctcgccaac atgtcgcacg agctgcgcac gccgctcaac agcctgatga tcctggccaa
     5761 gctgctggcc gagaaccaga gcggcaacct gaccgagaag caggtcgaat tcgcgcagag
     5821 catgtacgag gccggcaacg acctgctcaa gctgatctcc gacatcctcg acctgtccaa
     5881 ggtcgaagcc ggcaagatgg acatcagcat cgccccggtc gacctggtca ccctcgccaa
     5941 cgacatcgag cagatgttcc ggccgatcgc cgagcagcag gcgctcgact tcgcggtgga
     6001 ggtcgccccg gacaccccgg acccggtggt caccgacgag cgccgcgtcc agcaggtgct
     6061 gaagaacctg ctctccaacg ccttcaagtt caccgaggag ggctcggtgc ggctgcggat
     6121 cgggccgccg ccggctggga cccgcttcac cacccccggc ctgcgcgccg cgccggaggt
     6181 gatcgccttc tcggtggtcg attctggcat cggcatcgcc tccgaccagc tgctggtgat
     6241 cttcgaggcg tttcagcagg ccgacgggac caccaaccgc cgctacggcg gcaccggcct
     6301 cggcctgtcg atcagccgcg agatcgcccg cctgctcggc ggcgagctgc atgtcgagag
     6361 cgagcccgcc cggggcagcg tgttcaccct gctgctcccg gccgtggccg aaccacgcga
     6421 ggctggaccc gaggacgaga gcgccgccga acccgccggc gacgccgccg ccgaccccgc
     6481 tggttctggc gcggctgtcg cggcggccgt ggcggcggcg acgcggcgcg gggaggtcgc
     6541 tgtcgatggc cccgcctccg tgcccggcag cgacgcggcc cgggcggcgg ggctgggcct
     6601 gtcggccgcg gagacgatgc tggccggcaa gtgcgtgctg atcgtggacg acgacgtgcg
     6661 caacgtctac gcgctcacca gcgtgctgga gaaccgcggc atcgaggtgc tggtcgccga
     6721 ggacggccgc accggcatcg agctgctgaa gcgccggcca gccgtggacg tggtgctgat
     6781 ggacatcatg atgccggtga tggacggcta cgagaccacc cgggcgatcc gtcagctcga
     6841 cgggttccgg gacctgccga tcatcgcgct gaccgccaag gcgatgcgcg gcgaccgcga
     6901 gaagtgcctg gcagccggcg cttccgacta tattaccaaa ccggtcgata ccgaccaact
     6961 cctcgatatg ctgcgcgtgt ggttgtatcg atgagtcgtg gggaacgccc cttgccggga
     7021 gtgcgacggg aaggagcggc agtgggtggt tcggggggca cggtcccgcg gccggaagga
     7081 ccacaggacc gtgccaaggc agccgcgggg ccccccgatg cgacgggggg ttcgggggcc
     7141 agggtgctgt tggtggacga caaaccaggc aacctgctcg cgctgaccgc tgtcctcgaa
     7201 cagctcgatg tggacctggt cggggtcggg tccggggagg aggcgctgcg gcggctgctc
     7261 gtcgaggagt tcgccctgat cgtgctcgac gtgcagatgc cggggctgga cggcttcgag
     7321 acggcccggc tgatcaagca gcgcgagaag acccgctaca tcccgatcat cttcctgacc
     7381 gcgatcagcg gggcgcccga gcactacctg cgtggctacg ggctgggcgc ggtcgactac
     7441 gtctacaagc cgttcgcccc cgacatcctg cgtgccaagg tcggcgtctt cctggagctg
     7501 tggcagcgcg gcaccctgat cgagcggcag cgcgccgagc tggagcggcg cctggccgag
     7561 ctggaccggg cccacgagac gctcgcccgg caggccaacg acctggagcg ctccaacgcg
     7621 gcactgcagc gcttcgccga agtggccgcg cacgagctgc aagcgccgct gcacaacgcc
     7681 gccggcttcc tcgacctgct gctggcgcgc caccgcgacg ccctggacga ggaggcgacg
     7741 ctgctggccg agcgcgcggc ccgcggcgtc actgagatgc gggcgctcac cgggcggctg
     7801 ctcgactacg ccaaggccgc caccgacacg ctggcgaccg agcaggtggc gctgccggag
     7861 gtgctggagg cggccgagcg ggacctggcc gaaccgctcg gcgccgccgg tgccgtggtg
     7921 ctcgccgagc ggcttcccac ggtccccggc gaccggcgca tgctcatctg cctgttggtg
     7981 aacctgctcg acaacgcggt gaagttccgg ggggaggcgg ccccgctggt ccgcgtctcc
     8041 gccgaggccg ccccgggcct gtggcgggtg accgtgcgtg acaacggcgt cggcgtcgac
     8101 cccgacgagc tgccccgcct gttcaccgtg ttcgcgcgcc tgcacccgac cgccgaccgg
     8161 cccggccacg gcatcgggct ggcgacctgc cggcggatcg tcgagcgcca cggtggggtc
     8221 atctggtgcg agccggtccc gccggaggaa ggtggcggcg cgctgttccg cttcgtcctg
     8281 ccggccggtg ccgagtggcc gccggcaggg gacgcggagg agccgcgctg atgccgcgcg
     8341 tcctgatcgt ggacgacgac ccggattacc ggctgcttgt ccggctggcg ctggcgaccg
     8401 acccgcgttt cgaggtggtg ggtgaggcgg aggacggcaa cctggcggcc gccgcggcga
     8461 ccctctgcga agcggatctg gcgctgctgg actgctccat gcccggcgcc gacgccttcg
     8521 acgcgctgcc cgcgatccgc cgcgcccgcc cgagctgccg ggtggtgctg ctgagcggcc
     8581 acgccaccga ggatctgcgt gccgccgcct ggtcgagcgg ggtcctcggc tacctgggca
     8641 aggacgtgcg gccgagccgg ctcggggagg agctggtggc ggtgattggg atggtcgacg
     8701 cggtcgagtc ggtgctcaag gagacctcca cccagctggc gcacgacccg cgcagccccc
     8761 gggtcgcgcg cgccttcgtg cggcggacga tggagggctg gcgcctgcag cgtccgctcg
     8821 acaccgcggt gctgctggtg agcgagctgg tcaccaacgc ggtgctgcac gccggcaccg
     8881 acgtccgggt gatcctgcag ctcaccgagc acaccgcccg ggtcgaggtg gccgaccagg
     8941 ccagcgcgcc cccgccgctg ctgcggcgcg accacgaccc agcctccccc tccgggcgcg
     9001 gcctgcggat ggtctcgtcg ctggcgcgcc gctgggggac ctatcatgaa tccgcgggcg
     9061 gcaagaccgt ctggttcgaa ctggcccgcg acggcgggcc catcggcacc gggctggggt
     9121 gagcgcacag gtgacgggag aagccccgga agggcgcttg acgatgcagc gccgcgcgat
     9181 cgaacggctg gagggcgagc gtgaccggct cgaacggctg ctgctgcgcc tgccgtccct
     9241 gatgggcgag ttggacgtcg gcaagctggc ccagggggtg gtcgaggccg cggttgagct
     9301 gtccggcgcc cgtttcggcg cgttcgccgc cgccgacagc gacgaggaga ccgtggtgtt
     9361 cgtcggcgcc accgggccgc cgcccgcgtt cgccgagacg ccggccctga aggcggcgcc
     9421 gctgctcgcc ggcgccatct ggagcggcga ggccgtgcgg gtcgacgacg tgaccgcgtg
     9481 ggcctcccgc gacgagagcg cccgcgccta tggcgtgttc gccgacggcc gcctggtgcg
     9541 cagctggctg gccgtgccgg tgcgaggccg cggcggcgcc gtgctcggcg cgatctacct
     9601 cggccaccac cgcgcccacg ccttctccgg ccgccagcac gagctggtcg gcgggctctg
     9661 ctcgcagctc ggcgtggcga tggagaacgc ggagctgttc gccgagcggg gccggatcgt
     9721 ggccacgctg cagaagagcc tgctgccgcc gctgctgccg gacatccccg gggtcgacct
     9781 ggccgcgcgc taccggccga ccggggccac caacctggtc ggcggggact tcttcgacgt
     9841 cttcgagatg gccgacgacc gcacctgggg gctgatcctg ggggacgtga gcggcttcgg
     9901 cgccgaggcg gcggcgctga ccggcatcgc ccgctacacc gtgcgcgcca tcgccgggcc
     9961 cggcgacaag ccgagcgacg tgctcgccca gctcaacgag gcgatgctcc accaggggcc
    10021 ggcggagcgc ttctgcaccg cgctgtacgc gctggcgacc ctgccgtcgg cggcggaggg
    10081 cacgggcggc ggggtcgagg tgcggctcgc caacggcggc cacccgcccg ggctggtgct
    10141 gcgcgacgac gagtcggtgg agcggctcga agagggggcc ggcatcctgc tcggcgtctt
    10201 ctccgacccg gtcttcgccg acgaacaggt gaccctgcgg ccaggggacg cgctggtcct
    10261 ctacaccgac ggcgtcatcg aggcacgcga cctgacgggc acccagttcg gcgaggaccg
    10321 cctggcggcg gtgctggcca cctgcgccgg ccgctccgcc gacggcatcg cccgccgcgt
    10381 cgagctggcc gccctcgacc atcagggcaa tcagccgtca gacgacatcg ccatcgtggt
    10441 cctgcgcgcc cgccccaccg cccacccggc ttcctagcgg ctcgcgcacg gggtcgcgtc
    10501 ccgtcaggta gcagacccca ccgagcagca ggaaggtgac cgccccgaag ccgaggctga
    10561 ccaccgcctg gccgccgtgc agcaggccgg ccacgccgag gacggcgccc aggacggcga
    10621 agcacaggcc gccgatcttg tagaccatcc gctcctcttg gcttgcctgg ctcagctcgg
    10681 ggtgcttgcg cacgtgactt ggtagcgtaa ccgccgcccc ccggcgtgtc gatgtcagct
    10741 ctcgcgcagt tatcccctct gtcgcagtat gcgcgcctac ggcagtcaac ggttcgcggg
    10801 gggccgggtg cgccgtacgt tcctcagatc aagacaagcg agcgcacctc tgtcgccggg
    10861 gaggctccga tgcaccgcac acgactgccc gcaaccctgc tggccgccgc cctcatcgcc
    10921 atggggctgc tggtcgccgc ctcacggccg gtggcagcgg cggcgaccgt cctctatgtg
    10981 gaccagagca acgccagctg cagcgacagc ggcgccggca cgcagacggc gcccttctgc
    11041 accatcggcc agggcgcggc ggtcgccacc gccggccaga ccgtccaggt cgccagcggc
    11101 acctacaacg agctggtcac cgtcgcccac tccggcacct ccggcgcacc gatcgtgctg
    11161 accgccgcgc cgggggccag cgtcatcgtc agcggcaagg ccgacgcctt caagatcgcc
    11221 tccaagagct atgtgaccgt gcggaacttc accgtcacgg ggacctccag ctacggcgtc
    11281 ctggtggcca attcctcgaa catcgtcatc agcggcaacc acgtcagtgc cgccggccag
    11341 cgagtgtccg gttccacgaa gtacggcatc aagttcgtcg gctcgaccaa cgcgctggtg
    11401 tccggcaacg tctcccacga caactccgac gccggcatct tcctggacgc caccaccaac
    11461 ggcgtcgagg tcaccggcaa caccacctac ggcaacgccc gcggctacac ccgcgccgcc
    11521 cccggcatcg acgtccgcgg ccaccacaac accgtcgacc gcaacgtcag ccacaacaac
    11581 gaggactccg gcctgcagtt ctacagcggc tcccacgaca acctggtggt cgacaacctc
    11641 agttaccgca acggcgacca cggcatcgac gacctgcact cgaccggtca gcggatcctc
    11701 tccaacaccg tcttcggcaa cgtgaccgcc ggggtcaacc tcgagggcag ctccaccggc
    11761 gccaccgtgg agaacaacat cagcgtcgac aacggcgtca actcgccgcg caccaagagc
    11821 aacatccgcg tcgacgccac ctcgaccagc ggcaccacgg tcaactggaa cctcgtcgac
    11881 ttgagcgtct ccggccaggt catgtacgtg tggggcgcca agagctacgc cacgctgtcc
    11941 gccttccagt cagccagcgg ccaggagtcg cacggccgcc aggccaaccc gagctgggtc
    12001 gcggccgggt ccaacttcca cctgaaggcc ggctcgcccg cgatcgactc ggccaactcc
    12061 ggcgtcagcg gcgagaccag caccgacctg gagggcaagg cccgcaggga cgactcggcc
    12121 accgccaaca ccggcaccgg cccacggacc tacgacgacc gcggcgccta cgagttctga
    12181 gggagttcga gcgggaattc cagcaggtcg gtctcgcggg ccgggagggc cgggtcgagc
    12241 gggacccgga acgtcttgat ctcccccggg ctgaagcgcg cccgaaccgt gcgcccaacc
    12301 agtggcaggt cgatggtggc ctcggccggt cggccggcgc tctcataggc acggacgatc
    12361 aggtcgccgc cgggatcgcc aggagcggcg ggaccctcgc cctcggcgcg cttcagggcg
    12421 gtgacgacca ccgccgcggg cgtcaccgtg gcgagcgacc cgcgtggtgc cagcgaaccg
    12481 ggatgcgcgg attcgagcag cgcgaacggc tgctggttca gctcggcggc gaggcgcacc
    12541 acgcccgccg cgcgccagtc gccggcgtgc ggcaccagcc ggtaggtgaa gcgctgcacg
    12601 ccctggtcca ggtactcgta cggcacgccg ggccgcagcg gctccgggtc gtgccaggcg
    12661 gagacggggc tgcgcagcac cgtcatgccg atcgtcccgc cgccgacgtc gaagctgtac
    12721 ttgccgtcgt tgagcagcgc gagccccgcc cggcggccag caccggggag gtggccgctc
    12781 acgtcgaccc aggcctgcgc gggccgctcc tccccgtcgg tcgcgcggac gacctgcccg
    12841 cagggcacct ccgcggtcgc ggtcaccgcc tcaagggcgg tcgggaagcg cagcttcagc
    12901 agcttgagcc gctcccgcca gtcgacggtg acccgcacct ccaggtgtcg cgcctcccgc
    12961 ccgagcagga actcctccac cagcgtcgaa tgcccgtact cactctcaac ccgcagcacc
    13021 gagcggaccg gcccgtgctc gaccagccgc accgaccggc acgtgaaggt gccgacaact
    13081 tgtgggtcat cttctgggtc gcccacaccg gtgaacgacc gcaccccatg cccccaggtg
    13141 tcgctcccgt cgtcgatcac cacggcgtgc ggcgaggact cgggcggcaa cagctccacc
    13201 ccggacgcct tgtcccgcag gctcgccagc cagccagtgg ccgcgtccac ccgcagcaag
    13261 agatgctcgc cctccaggat gtccaggctt ggcgtcctcg gcccatttgc cgggggatcc
    13321 accacacctc ctccccggct cattatatcg ttcgcaaacc aactctgcaa ggcgctatcc
    13381 aacgctttcg agcgcaaacg gaagagaacc cagccaagcg ccggcagcgc cgctgcgaac
    13441 accacgcgct cgcgccacgg cacggtggcg tgtgagcggg tcgcctggct cggcaccggg
    13501 tcgccgccgt cgtcggtgac cagcagggcg ctgccgcgcg caacctcgaa ctcgattgcg
    13561 gcgcgcaccg gccagggatg cgggttgaag gccaccaggg gcgtggtgcc cggctcctca
    13621 gggatgccga cccggcccgc gaccgcctgc gcggcgccgt acagcgcccg gctggcgatc
    13681 gcggcggcct cgccgagctg gtcgcgggcg tcgtcgtagg cggcggcgac cgcggtgccc
    13741 gccaggacgt cgtgcgcctg gttgaacagc agctgcttcc aggcgtgggt cagtgcggcg
    13801 gccgggtatg ggcaggcacc ctcgctgagg accgccgcac ccaccgccca cttctccgcc
    13861 gcgagcagca ggtgctcgac gcggcggttc tgccgcttga tgccggagtg cgcggcgtag
    13921 cagccgaccg cgtgcggctg cagctcgccc gcgacgaccg gcaggtggtc gccgtcggcg
    13981 gcctggagcc ggtcgaagaa ctcccgcgcg cgggagcact ccagcggcgg gaacccgtcc
    14041 gtgccgccga gccggcggat cgagtcgagg ttcgccctgg tcggcccgcc gccgtggttg
    14101 ccgacgccgt agcagcaggc cacctcgtgg tccgcgccga cctcggcgag ctgcgccagc
    14161 gccgcgcgca ggtgctcggc caggtcgatg ccaggcgtct ggtactcatg cgggacccgg
    14221 aaggcgagca cccgcgatcc gtccgccgac tgccaccaga atcccgcacc cgccaacggc
    14281 agctcccccg gccctggccg caggaacagg tagctgtcca gcccgctctt gcgcagcagt
    14341 tgcggcagcg tggcgggatg cccgaacggg tccaggttgc acccgacggt cgcggtgatc
    14401 ccaagcttgt cgagcagcca gcgctgcccg tagagggcct ggcgcaccag cgactccccg
    14461 catggcaggt tgcagtccgc ctcgacccac cacccacccg cgatctcgaa acgcccctcg
    14521 gcgacccggc gccggatctc cccgaacagc tccgggtcgt gctcctccac ccaggcgagg
    14581 aacgcgaccg agtcgcaggt gaacacgaac tccgggtact cccccatgcg ctccagcacc
    14641 gaccggaagg tcgccagcac cgcctggtag ccctccggcc agcgccacag ccacaccggg
    14701 tcgatgtggc tgttgccgat caggtggagg gggcggcgct cccgcctcgc atcgccgtcg
    14761 gaccgctcca ccacagcgcg gatcagggcc ggcgcagcgg ctcgtcgggc gccggggctg
    14821 ccgcctcggt cccagcgggt gtcgagggag ccggctggcg gcggaaggcg ccgagaaggg
    14881 tgaccgcgag ggtcgcgcag atcagcccgg cggcggcgga cagcaggtag ccgacgtgga
    14941 cggcgctgac gaaggcgtcg ccgacgaccc g
//
LOCUS       DASWDC010000091         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_853472, whole genome shotgun sequence.
ACCESSION   DASWDC010000091 DASWDC010000000
VERSION     DASWDC010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853472"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VF995_03045"
     CDS             <1..386
                     /locus_tag="VF995_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9342577.1"
                     /translation="QRLSNVGLLLALLVAGCSLAIAVSAGLIERKRPFALLRLSGVPV
                     SSLNRVVLAEAAAPLLLVAAASAGLGLGVAAMILKVVSVSGWHPPAAGYWYALAAGIS
                     FALLTVVATLPLLKRVTSLETARFE"
     gene            474..893
                     /locus_tag="VF995_03050"
     CDS             474..893
                     /locus_tag="VF995_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX9342578.1"
                     /translation="MMNYRIGQVAELLGVSADTVRRWADAGRLGARRTEGGQRVIEGA
                     ALAEFVTGLRESSAAERDELVMAQSARNRFAGIVTRVVKDTVMAQVELQAGRYRVVSL
                     MSREAADELALAPGMLAVAAVKATNVVVELPSQGSSR"
     gene            924..1706
                     /gene="modA"
                     /locus_tag="VF995_03055"
     CDS             924..1706
                     /gene="modA"
                     /locus_tag="VF995_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9342579.1"
                     /translation="MLRSRPSLRHAALLLLVAVLATACGGKKPTAGSGSSSTEIKVFA
                     AASLTAAFTKLGTDFTAAHPGVKVTFNFAGSQALATQVQQGAPADVFASADTTNMDKV
                     KSLVGTPQLFASNLLQIVVAPGNPKGIKSLADLARGDLKVVLEAPEVPAGKYAGQALA
                     AQHVTVKPVSLEDNVKAVVTKVSLGEADAGIVYVTDVAAAKAKVAGVDIPKDQNVPAT
                     YPIAVVTASKHAQDAQAFMDLVRSTQGQQVLQSFGFLPPPTS"
     gene            1711..>2003
                     /locus_tag="VF995_03060"
     CDS             1711..>2003
                     /locus_tag="VF995_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX9342580.1"
                     /translation="MAVTARRQRTARKTTGGAAAGAGRAPRAFVALVGLAVAFLAIPL
                     AGLLVRTPWGSLATDLRDPAVLTALRLSLVCSVGAVAASTLVGVPLAWLLARVP"
BASE COUNT          274 a          741 c          680 g          308 t
ORIGIN      
        1 tgcagcgcct cagcaacgtc ggcctgctgc tggccctgct ggtcgctggc tgcagcctcg
       61 ccatcgccgt cagcgcgggc ctgatcgagc gcaagcgtcc cttcgccctg ctgcgcctga
      121 gcggcgtccc ggtgtcgagc ctcaaccggg tcgtcctcgc ggaagccgcc gcgccgctgc
      181 tgctggtcgc cgccgccagc gccggcctcg gcctcggcgt cgccgccatg atcctgaagg
      241 tggtcagcgt ctcagggtgg catcccccgg ccgccggcta ctggtatgcc ctggccgccg
      301 gcatctcctt cgccctactg acggtcgtcg cgaccctgcc gctgctcaaa cgcgtcacct
      361 cgctcgaaac cgcccgcttc gagtagaacg ccaaggaaag caaccttgca tatgcaaggt
      421 ttgagcgtgg attgctttga aatgcggctg cgaaccgcta gcatcggccg cagatgatga
      481 actatcggat cggccaggtc gcggagctgc tcggcgtcag cgccgacacc gtgcgccgct
      541 gggcggacgc cggccggctc ggggcgcggc gcaccgaagg cggccagcgc gtgatcgagg
      601 gcgccgccct ggctgaattc gtcaccgggc tgcgggagtc gtcggcggcc gagcgcgacg
      661 agctggtgat ggcgcagtcg gcccgcaacc ggttcgcggg gatcgtcacc cgggtggtca
      721 aagacaccgt catggcccag gtcgaactgc aggccggccg ctaccgggtg gtctcgctga
      781 tgtcgcgcga ggccgccgac gagctggcgc tcgcccccgg catgctcgcg gtcgcagccg
      841 tcaaggcgac caacgtcgtg gtcgaactgc cgtcccaggg ttccagtcgt tgagttgttg
      901 ttgagttgtt gaggagggca tccgtgcttc gctcgcgtcc cagtctccgc catgccgccc
      961 tgctcctgct ggtggcagtg ctcgccaccg cctgcggcgg gaagaagccc accgctggga
     1021 gcggaagctc ctccacggag atcaaggtgt tcgccgccgc ttccctgacc gccgcgttca
     1081 ccaagctcgg gaccgacttc accgccgccc accccggggt gaaggtcacc ttcaacttcg
     1141 ccggcagcca ggcgctcgcc acccaggtcc agcagggcgc cccggctgac gtgttcgcct
     1201 ccgccgacac caccaacatg gacaaggtca agagcctcgt cgggaccccg cagctgttcg
     1261 ccagcaacct gctccagatc gtggtcgcgc ccggcaaccc gaaggggatc aaaagcctcg
     1321 ccgacctggc ccgcggcgac ctcaaggtgg tgctggaggc gccggaggtg cccgccggca
     1381 agtacgccgg ccaggccctc gccgcccagc acgtcaccgt caaacccgtc tcgctggaag
     1441 acaacgtcaa ggccgtggtg accaaggtct ccctcggcga ggctgacgcc gggatcgtct
     1501 acgtgaccga cgtcgccgcc gccaaggcca aggtcgcggg cgtcgacatc cccaaggacc
     1561 agaacgtacc cgccacctac ccgatcgcgg tggtcaccgc cagcaagcac gcccaggacg
     1621 cccaggcgtt catggacctg gtccgttcca cccagggcca gcaggtgctc cagagcttcg
     1681 ggttcctgcc gcccccgacc tcgtgacggc atggccgtga ccgcgcggcg ccagcggact
     1741 gccaggaaga cgaccggcgg ggcagcggcc ggggccgggc gggcgccgcg cgccttcgtc
     1801 gcgctggtcg ggctcgcggt cgcgttcttg gcgatcccgc ttgccgggct gcttgtccgc
     1861 acgccgtggg ggagcctcgc caccgacctg cgcgatccgg cggtgctgac cgcgttgcgg
     1921 ctgtcgctgg tgtgctccgt cggcgcggtg gccgcctcga cgctggttgg ggtgccgctc
     1981 gcctggctgc tcgcccgcgt ccc
//
LOCUS       DASWDC010000092         4487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_854965, whole genome shotgun sequence.
ACCESSION   DASWDC010000092 DASWDC010000000
VERSION     DASWDC010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854965"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /gene="aspS"
                     /locus_tag="VF995_03065"
     CDS             complement(<1..1056)
                     /gene="aspS"
                     /locus_tag="VF995_03065"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012182003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX9342581.1"
                     /translation="MRSHACGSLRAADTGTEVRLAGWVDRRRDHGGVVFLDLRDASGV
                     VQVVVHPETAPAATDTAHTLRNEDCIRIRGTVAPRPAGNENPDLPSGAVEVTATELEV
                     LAPSATPPFPVADRVDADETLRLRYRYLDIRRPSVRQALVLRAHANAVLRRMLEERGF
                     LEVETPYLTRSTPEGARDFLVSTHLQPGEFYALPQSPQLFKQLLMVAGVERYYQIVRC
                     FRDEALRADRQPEFTQLDIELSFLDEEDIYQLGEDLMTAVLREVLGVALPTPFPRLSF
                     AEAMRRFGTDKPDLRYGMELADLGEVFAAGGVGVFTRALEAGGSVLAIRIPRGGDLTR
                     RELDTLTDWAKARGARGL"
     gene            complement(1083..2402)
                     /gene="hisS"
                     /locus_tag="VF995_03070"
     CDS             complement(1083..2402)
                     /gene="hisS"
                     /locus_tag="VF995_03070"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005513519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX9342582.1"
                     /translation="MTARFEAPKGTYDHLPPHAAQRAAVAGVMLEQARLAGYQRVETP
                     IFEDTALFARGVGESTDVVQKEMYTFTDKGGRSLTLRPELTAGVLRALIEHNRVGATA
                     GLVKVACYGPQFRYERPQAGRQRQFSQVDIEAVGSADPAVDADVILVGEEVFRRLGAE
                     VTLLLTSLGDATCRPLYRERLLGFLRGLGDRLPAEARARAEQNPLRVLDMKDPSIQEL
                     TAAAPLLRDSLCDTCQAYHEAVCELLTAAGVKWVEAPRLVRGLDYYLRTTFEWQAEGM
                     DAAQNALGGGGRYDGLSELLGGPPAPGIGWALGVDRTMLALERAGRLPETQEALAVLV
                     VPLAGADQTRATGVGVTLVRDLRRAGIAADLPYAERPLRRHLQAAAKRGARAVVLLGD
                     EELAAGQATVRDMAAHDQTQVPLDAVVTEIRGILLSAPPTDDPGAAT"
     gene            complement(2399..3109)
                     /locus_tag="VF995_03075"
     CDS             complement(2399..3109)
                     /locus_tag="VF995_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9342583.1"
                     /translation="MLIAGFPAGSFQTNCYLVATGPGSEALVIDPGQDALPGIEQLCA
                     RHRLNLVAALLTHGHLDHVWTVAPLAGAHGIPAFIHPDDQYLLSDPLAGFGPEARDWF
                     ASLGLDLKEPDDVRELADGQTIALAGVELTVDHTPGHTRGHVTFRAPADPGEPGTLFA
                     GDLVFAGSIGRTDLPGGDSRAMRSSIATRFLTLPDETEVLPGHGPETTVGQERATNPF
                     LAGLTPEPAVRRAAGGAG"
     gene            3368..4204
                     /locus_tag="VF995_03080"
     CDS             3368..4204
                     /locus_tag="VF995_03080"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9342584.1"
                     /translation="MTKQTRQRELARLRARREAERHRTQRKRALLTYGVLGVVVLLLG
                     TVGAIALANRPKHKAAAATTTSTTTTTIPPTGARIDAPAAPATVACGAKLPPRHQHPS
                     FKKAPTSRLDPSKTYVVTLKTSCGVIKLQMDPKTAPIAAANFVFLAQKGFYDATWFHR
                     IVPGGAAGLAVIQGGDPKGTGQGGPGYSIKDEPPKKIKDPYKQYTIAMAKTSQPNSAG
                     SQFFVNSGDNAKLPADYALFGKVISGQKVVDKIAAIPVGGSSGDTPLQSAWIEQATVQ
                     VS"
     gene            complement(4235..>4487)
                     /locus_tag="VF995_03085"
     CDS             complement(4235..>4487)
                     /locus_tag="VF995_03085"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020310871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX9342585.1"
                     /translation="LDTGGGVLVVSQFTLHGDARRGRRPSYIDAARPEHAEPLVDAVA
                     RCLAGSGLKVATGRFRAYMLVESVGDGPITILLDSAKTF"
BASE COUNT          643 a         1549 c         1703 g          592 t
ORIGIN      
        1 gaggcccctg gcgccccggg ccttggccca gtcggtgagg gtgtccagct cacggcgggt
       61 gaggtcgccg cctcgtggga tcctgatcgc gagcaccgag ccgccggcct ccaaggcgcg
      121 ggtgaacacc ccgacgccgc cggcggcgaa cacctcgccg aggtcggcca gttccatgcc
      181 gtagcgcagg tcgggcttgt cggtgccgaa gcggcgcatc gcttcggcga agctgaggcg
      241 cgggaagggg gtcggcagcg cgacgccgag gacctcgcgc agaaccgccg tcatcaggtc
      301 ctcgccgagc tggtagatgt cttcttcgtc gaggaacgac agctcgatgt cgagctgggt
      361 gaactcgggc tggcggtcgg cgcgcaacgc ctcgtcgcgg aagcagcgca cgatctggta
      421 gtagcgctcg accccggcga ccatcagcag ctgcttgaac agctgcgggg actgcggcag
      481 cgcgtagaac tcgcctggct gcaggtgggt ggagaccagg aaatcgcggg cgccttccgg
      541 ggtggagcga gtcaggtagg gggtttcgac ctcgaggaag ccgcgttcct ccagcatgcg
      601 gcgcaggacc gcgttggcgt gggcgcgcag caccagcgcc tggcgcaccg aggggcggcg
      661 gatgtcgagg tagcggtagc gcagccgcag ggtctcgtcg gcgtcgacgc ggtcggcgac
      721 cgggaacggc ggggtcgcgg agggggcgag gacctccagt tcggtggcgg tcacctcgac
      781 cgcgccgctg ggcaggtcgg ggttctcgtt gccggcgggg cgcggggcga ccgtgccgcg
      841 gatgcggatg cagtcctcgt tgcggagggt gtgggcggtg tcggtggcgg cgggggcggt
      901 ctcagggtgg acgaccacct ggacgacccc ggaggcgtcg cgcaggtcga ggaagaccac
      961 cccgccgtgg tcgcggcggc ggtcgaccca gccggccagg cgcacctcgg tgccggtgtc
     1021 ggcggcgcgg aggctgccgc aggcatgcga gcgcatggca cccagcaatg gacgggggcg
     1081 ggtcacgtgg cggctccagg atcgtcggtg ggcggggcac tgaggaggat gccgcggatc
     1141 tcagtgacga ccgcgtccaa cggcacctgc gtctggtcgt gggcggccat gtcgcggacg
     1201 gtggcctggc cggcggccag ttcctcgtcg ccgaggagca ccaccgcgcg ggcgccgcgc
     1261 ttggcggcgg cctgcaggtg gcggcgcagc ggccgctcgg cgtagggcag gtcggcggcg
     1321 atcccggcgc ggcgcaggtc gcggaccagt gtgacgccga ccccggtcgc tcgggtctgg
     1381 tcggcgccgg ccagcggcac gaccagcacc gccagcgcct cctgcgtctc cggcaggcgg
     1441 ccagcgcgct ccagggcgag catggtgcgg tcgacaccga gcgcccagcc gatcccgggt
     1501 gccggcgggc cgccgagcag ctcggacagt ccgtcgtagc gaccgccgcc gccgagggcg
     1561 ttctgggcgg cgtccatgcc ctcggcctgc cactcgaagg tggtgcgcag gtagtagtcc
     1621 aggccgcgga ccagccgcgg cgcctcgacc catttcacgc ccgccgccgt gagcagctcg
     1681 cagacggcct cgtggtaggc ctggcaggtg tcgcagaggc tgtcgcgcag cagcggcgcg
     1741 gcggcggtca gctcctggat ggaggggtct ttcatgtcga gcacccggag cgggttctgc
     1801 tcggcgcggg cgcgcgcctc ggcggggaga cggtcgccga gcccgcggag gaatcccagg
     1861 agccgctcgc ggtacagcgg ccggcaggtg gcgtcgccga ggctggtgag cagcagcgtc
     1921 acctcggcgc cgaggcggcg gaagacctcc tcaccgacca ggatgacgtc ggcgtccacc
     1981 gccgggtcgg cgctgccgac cgcttcgatg tcgacctggg agaactggcg ctgccggcct
     2041 gcctggggtc gttcgtagcg gaactgcggc ccgtagcagg cgaccttgac caggccggcg
     2101 gtcgcgccga cgcggttgtg ctcgatcagc gcgcgcagca cgcccgcggt cagctccggc
     2161 cggagggtca gggagcggcc gcccttgtcg gtgaaggtgt acatctcctt ctggacgacg
     2221 tcggtggact cccccacccc gcgggcgaac agggccgtgt cctcgaagat cggcgtctcg
     2281 acgcgctgat agccagccag ccgcgcctgc tccagcatca ccccggcgac cgccgcccgc
     2341 tgcgccgcgt gcggcgggag gtggtcgtag gtgcccttgg gcgcctcgaa gcgggcggtc
     2401 atccggcacc tccggcggca cgccggaccg cgggttccgg ggtgagcccg gcgaggaacg
     2461 ggttggtggc ccgctcctgg ccgacggtgg tctcgggtcc gtggccgggc aggacctcgg
     2521 tctcgtcggg gagggtcagg aaccgggtcg cgatcgagct gcgcatcgcg cggctgtcac
     2581 cgccgggcag gtcggtgcgg ccgatcgacc cggcgaacac caggtcgccg gcgaacagcg
     2641 tccccggctc gccgggatcc gccggtgccc ggaaggtgac gtgcccgcgg gtgtggcccg
     2701 gggtgtggtc gacggtcaac tcgaccccgg ccagggcgat ggtctgcccg tcggccagct
     2761 cgcggacgtc gtcgggctct ttgaggtcga ggccgaggct ggcgaaccag tcgcgcgcct
     2821 ccgggccgaa cccggccagc gggtcgctga gcaggtactg gtcatccggg tggatgaacg
     2881 ccgggatgcc gtgggcgccg gccagcggcg ccactgtcca gacgtggtcc aggtggccgt
     2941 gggtgagcag cgcggccacg aggttcagcc ggtggcgggc gcacagctgc tcgatccccg
     3001 gcagggcgtc ctgtccggga tcgatgacca gcgcctcgct gcccggtccg gtggcgacca
     3061 ggtagcagtt ggtctggaac gatcctgcgg gaaatccggc gatcagcatt ggcggcacaa
     3121 tccggttcgg gaactgggtc gagggcgagt ctaccggaag ccaaattccc gtcacttgag
     3181 ttatccacag ggaccgacgg aagctcgtgc gcccaagtag actcgggacg cgcaagcgac
     3241 cggctcggcc ggcctcccac ggacggcatc gctccgctcg cctgcgtgct cggccggccg
     3301 ctggtgggag gccccgcgct caaagctttt gacgcagctt tcatttcgta gcctcggagg
     3361 accttgcatg acgaagcaga cacgccaacg cgagctggcc aggctccgcg cccggcggga
     3421 ggccgagcgc caccggacgc agcgcaagcg ggcgctgctg acctacggcg tgctcggcgt
     3481 ggtcgtgctg ctgctgggca cggtgggcgc catcgcgctc gccaaccggc ccaagcacaa
     3541 ggccgccgcg gccaccacga cctcgacgac caccacgacc atcccgccga ccggcgcccg
     3601 gatcgacgcg ccggccgccc cggcgacggt cgcctgcggc gccaagctgc cgccccgcca
     3661 ccagcacccc agcttcaaga aggcgccgac cagccgcctc gacccttcca agacctacgt
     3721 ggtcacgctg aagacctcct gcggcgtcat caagctccag atggacccca agaccgcgcc
     3781 gatcgcggcg gcgaacttcg tgttcctggc acagaagggc ttctacgacg ccacctggtt
     3841 ccaccgcatc gtccccggcg gcgccgccgg actcgcggtc atccagggcg gcgaccccaa
     3901 gggcaccggc cagggcggcc ccggctacag catcaaggac gaaccgccca aaaaaatcaa
     3961 agacccatac aagcaataca ccatcgccat ggccaagacc tcccagccga attccgctgg
     4021 cagccagttc tttgtcaaca gcggcgacaa cgccaagctc ccggccgact acgccctgtt
     4081 cggcaaggtc atctccggcc agaaggtggt cgacaagatc gccgcgatcc cggtcggcgg
     4141 cagcagcggc gacaccccgc tgcagtcagc ctggatcgag caggccaccg tgcaggtgag
     4201 ctgacccgga cggctgacgg ctgaaggctg acggctagaa ggtcttggcg ctgtcgagca
     4261 ggatcgtgat cgggccgtcg ccgaccgact cgaccagcat gtaggcgcgg aagcggccgg
     4321 tggcgacctt gaggccgctc ccggcgagac accgggcgac cgcgtcgacc agcggctcgg
     4381 cgtgctcggg ccgggctgcg tcgatgtagg aggggcggcg gccgcggcgg gcgtcgccgt
     4441 ggagggtgaa ctggctgacc accagcaccc cgccgccggt gtcgagc
//
LOCUS       DASWDC010000093         5589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1274241, whole genome shotgun sequence.
ACCESSION   DASWDC010000093 DASWDC010000000
VERSION     DASWDC010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274241"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VF995_03090"
     CDS             <1..276
                     /locus_tag="VF995_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006087716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9342586.1"
                     /translation="ERGFRAQPDNQPDNRHAGGDIGLGLYLCRELARGIGAQVLLERS
                     GPDGSVFSVRLPAPATTPLGLSTQTQRDASPGPGTSPAPLAKEQVAS"
     gene            complement(315..833)
                     /locus_tag="VF995_03095"
     CDS             complement(315..833)
                     /locus_tag="VF995_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9342587.1"
                     /translation="MVEDLQDSRRTEPAYIADERPMLEAWLEFHRMTLQLKCEGLDRA
                     GLVARPVPTSLLSLHGLVRHMADVERNWFTRVLLRASDTKPLFYDLAVDDSELVPLDN
                     ADFDHDLAAWQQECAASREHAARFALDDTGLRRGEEVSLRWIYVHMIEEYARHNGHAD
                     LIRELVDGAVGW"
     gene            complement(837..1664)
                     /locus_tag="VF995_03100"
     CDS             complement(837..1664)
                     /locus_tag="VF995_03100"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX9342588.1"
                     /translation="MSVTFEVPERLTAAAAAVTPRLPERPLVALVLGSGLGGLADQVE
                     GAVRIPAAEVPGLPVPRVPGHIGALVAGRLGGVPVLVLAGRVHLYEGWSAAEAAFATR
                     LAALLGCRTLLATNAAGGINLDFEPGDLMALRDHISLLPDNPLRGSATFVDLAGAYDK
                     DLRAEAARIAAERGIPLREGVYLAAPGPSYETPAEIAMFRSWGADAAGMSTVPEVIAA
                     RAHSLRVAAVSAITNVHRVGGSPVTHQEVLEVTARIQPRFRDLVLALLPAAADKPEE"
     gene            complement(1675..2046)
                     /locus_tag="VF995_03105"
     CDS             complement(1675..2046)
                     /locus_tag="VF995_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEX9342589.1"
                     /translation="MPVLGASEILGAIIQDDRPWGNFRQFTHNQESTVKILTVDAGQT
                     LSLQQHEKRDELWIVLDTGLRVQVDDDVFEPAVGDEILIMRGSLHRLSSAGPRGRVLE
                     IAFGFFDESDIIRLEDAYNRA"
     gene            complement(2079..2258)
                     /locus_tag="VF995_03110"
     CDS             complement(2079..2258)
                     /locus_tag="VF995_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX9342590.1"
                     /translation="MAVSADLLAILACPNCRGEVDYREAEQVIECRQCHYRYPVRDDI
                     PIMLVDEAEKPASSS"
     gene            complement(2312..3703)
                     /gene="manB"
                     /locus_tag="VF995_03115"
     CDS             complement(2312..3703)
                     /gene="manB"
                     /locus_tag="VF995_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653455.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX9342591.1"
                     /translation="MAAADLGDLSKVFKAYDVRGEYPDQLDEGLAERIGRAFIPLTGA
                     RTLAIGQDMRPSSGPLADALAKGATSQGADVLRIGLCSTDMLYYVSGSLDVAAIMLTA
                     SHNPSRYNGLKFCRPGAVPVGEESGLTDLRNMVAAGEPATDAAQPGQVEAREDLVDRY
                     AAHVRGFVDVSALRPLKVVADAGNGMAGFVLPRIFERLPCQLVPMYFELDGNFPNHPA
                     DPINPDNLKDLQARVLEEGADVGLAFDGDADRVFLVDERGGIVQSSLVGALVARTMCE
                     REPGATVLYNLICSHVVPETIREAGGTPVRTRVGHSFIKAIMADTGAIFGCEHSGHYY
                     FRDNYRADSGLIAALVTLEVLSRAEAPLSRVLAPYRRYADSGEVNRRVAGDDPKAVIE
                     QVAEAFDGQRQDRTDGLTVEADADKDAGGWWFNLRPSNTEPLLRLNVEADTDERMAAV
                     RDRVLGLIDGESA"
     gene            4238..4603
                     /locus_tag="VF995_03120"
     CDS             4238..4603
                     /locus_tag="VF995_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX9342592.1"
                     /translation="MQPAGTGESAFMRLVEAAVASIPEEFRQRLDNVEFVVEEIPRGD
                     EVPGEGIPLGLYLGVPLTERNAGYYGVLPDRIVIFRRPIEARARSEAALAALVREVVI
                     HEVAHYFGIDDDRLDELGW"
     gene            complement(4613..>5589)
                     /locus_tag="VF995_03125"
     CDS             complement(4613..>5589)
                     /locus_tag="VF995_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342593.1"
                     /translation="IANPDADDAVVDLVPHLPNGALVAETDEIFVPRRSTVARPVKFS
                     AAQGLPFSVEAVARTGRFVVGGVITHQGQPSTFVPGQSTPRSSWSFAGGFSGNGHQTT
                     LLVSNPSTSPLQVSARVIGDKGAFDLHDPAFDKPIPSGALAQIPINPPTTASSPASGG
                     SFGLQVSSRDGTHYIAALRVSSALAGAQQVAYVDPGTALPDRSWLVPGATGGKVVLVN
                     TSDSAVTAQLIPVGGQPSGQAATRNGGKAATGTGRGATAPPPTQALTLPPGRVVVRDV
                     PGQVDALDVEAGEPGVLVALLGEGEAIPGAVVGGVPAGGPVVQGPAAS"
BASE COUNT          782 a         1907 c         2088 g          812 t
ORIGIN      
        1 gagcgcggct tccgggccca gccggacaac cagccggaca accgccatgc cggtggcgac
       61 atcgggctcg gcctctacct gtgccgggag ctggcccgcg gcatcggcgc gcaggtcctg
      121 ctggagcgct ccggccccga cggatcggtg ttcagcgtgc ggctgccggc cccggcaacc
      181 accccgctgg gcctgagcac ccagactcag cgggacgcga gcccgggacc cggcaccagt
      241 cccgcgccgt tggcgaaaga gcaggtcgcc tcatagcacc gcgcagccgt ccggaatagc
      301 ggtcacggtt gcggtcacca gccgacggcg ccgtcgacca gttcacggat gaggtcagcg
      361 tggccgttgt ggcgggcgta ctcctcgatc atgtggacgt agatccagcg cagggagacc
      421 tcctcgccac ggcgcaggcc ggtgtcgtcg agcgcgaagc gggcggcgtg ctcgcggctg
      481 gcggcgcatt cctgctgcca ggccgccagg tcgtggtcga agtcggcgtt gtcgagcggg
      541 accagctcgc tgtcgtcgac agcgaggtca tagaagagcg gcttggtgtc ggaggcgcgc
      601 agcaggaccc gggtgaacca gttgcgctcg acgtcggcca tgtggcgcac caggccgtgc
      661 agcgacagca acgaagtcgg gaccgggcgg gcgaccaggc cggcgcggtc gaggccctcg
      721 cacttcagct gcagcgtcat gcggtggaac tccagccagg cttccagcat cgggcgctcg
      781 tcggcgatgt aggccggctc ggtgcggcgt gagtcctgca ggtcttcgac catcgttcac
      841 tcctccggtt tgtcagccgc ggcgggcagc agcgccagca ccagatcgcg gaaacgcggc
      901 tggatacggg cggtgacctc aaggacttcc tggtgggtga ctggggagcc gccaacccgg
      961 tggacgttgg tgatggcgga gacggcggcg acgcggaggc tgtgggcgcg ggcggcgatc
     1021 acctcgggga cggtggacat gccggccgcg tcggcgcccc agctgcggaa catggcgatc
     1081 tcggcggggg tctcgtagct cgggccgggg gcggcgaggt agacgccttc gcgcaggggg
     1141 atgccccgct cggcggcgat gcgggcggcc tccgcgcgca ggtccttgtc gtaggcgccg
     1201 gccaggtcga cgaacgtggc gctgccgcgc agggggttgt ccggaaggag gctgatatgg
     1261 tcgcgcaacg ccatcaggtc gccaggctcg aagtccaggt tgatcccacc ggccgcgttg
     1321 gtggccagca aggtgcggca gccgagcagg gccgccaggc gcgtggcgaa ggccgcctcg
     1381 gccgccgacc agccctcgta caggtggacc cgcccggcca gcaccagcac cggcacgccg
     1441 ccgagccggc cggccaccag ggcaccgatg tgcccgggga cccgtggcac cggaaggcct
     1501 ggcacctcgg cggctgggat ccgcaccgcg ccctccacct ggtcggccag gccgcccaga
     1561 cccgagccga gcaccagcgc gaccagcggc cgctccggca gccgcggcgt caccgccgca
     1621 gcggcggcgg tgagccgctc agggacctcg aaggtgacgc tcacgatagc ggcctcaggc
     1681 gcggttgtag gcgtcctcca agcggatgat gtcgctctcg tcgaagaacc cgaaggcgat
     1741 ctcgagcacc cggccgcggg gtccggcgga ggagaggcgg tgcagcgagc cgcgcatgat
     1801 gaggatctcg tcgccgacgg cgggctcgaa gacgtcgtcg tcgacctgga cgcggaggcc
     1861 cgtgtccagg acgatccaga gctcgtcgcg cttctcgtgc tgctggaggc tgagcgtctg
     1921 gccggcgtcg accgtcagga tcttgaccgt cgactcctgg ttatgggtga actgccggaa
     1981 gttcccccat ggacggtcgt cctggatgat cgcgcccagg atctccgaag cgccgaggac
     2041 tggcatcgca gtggttcctt tcacgttcgc ggtagcgctc aggagctgga cgccggcttc
     2101 tcggcctcgt cgaccagcat gatcgggatg tcgtcacgga ccggatagcg gtagtggcac
     2161 tggcggcact cgatcacctg ctccgcctcg cggtagtcga cctcgccgcg gcagttcggg
     2221 caggccagga tcgcgagcag gtcagcactc acagccatgt ggactctcct caatctccag
     2281 tggacgtcga aacttcgctt gaagcttcag cttaagccga ctcgccgtcg atcaggccga
     2341 gcacgcggtc gcgcaccgcg gccatccgct cgtcggtgtc ggcctcgacg ttgagccgca
     2401 gcagcggctc ggtgttgctc gggcgcaggt tgaaccacca gcccccggcg tccttgtccg
     2461 cgtccgcctc gacggtgagg ccgtcggtgc ggtcctgccg ctggccgtcg aacgcctcgg
     2521 cgacctgctc gatgaccgcc ttggggtcgt cgccggcgac ccggcggttg acctcgccgg
     2581 agtcggcgta gcggcggtag ggcgccagca cccgcgacag cggcgcctcg gcgcgggaca
     2641 gcacctccag ggtcaccagc gcggcgatca ggccggagtc ggcgcggtag ttgtcgcgga
     2701 agtagtagtg gccggagtgc tcacagccga agatcgcgcc ggtgtcggcc atgatcgcct
     2761 tgatgaacga gtggcccacc cgggtgcgga ccggggtgcc gccggcctcg cggatcgtct
     2821 ccggcaccac gtgggagcag atcaagttgt agagcacggt cgcgcccggc tcccgctcgc
     2881 acatggtccg cgccaccagc gcgccgacca ggctggactg gacgatgccg ccccgctcgt
     2941 cgaccaggaa cacccggtcg gcgtcgccgt cgaaggcaag cccgacgtcg gcgccctcct
     3001 caaggacccg ggcctgcagg tccttgaggt tgtcggggtt gatcgggtcg gcgggatggt
     3061 tggggaagtt gccgtccagc tcgaagtaca tcgggaccag ctggcacggc agcctctcga
     3121 agatcctggg gagcacgaag ccggccatgc cgttgccggc gtcggcgacc accttgagcg
     3181 gccggagggc ggacacgtcg acgaagccgc gcacgtgcgc cgcgtagcgg tcgaccaggt
     3241 cctcgcgggc ctcgacctgg ccgggctggg cggcgtcggt cgccggctcc cccgccgcga
     3301 ccatgttcct gaggtcggtg aggccggact cctcccccac cgggaccgcg ccggggcggc
     3361 agaacttcag gccgttgtag cgcgaggggt tgtgggaggc ggtgagcatg atcgcggcca
     3421 cgtccagcga cccggagacg tagtagagca tgtcggtcga gcacagcccg atccgcagca
     3481 cgtcggcgcc ctggctggtg gcgcccttgg cgagcgcgtc ggccagcggg cctgacgacg
     3541 ggcgcatgtc ctgcccgatc gccagcgtcc tggcgccggt cagcgggatg aacgcccgcc
     3601 cgatccgttc cgccaggccc tcgtcaagct ggtcggggta ctcgccccgc acgtcatagg
     3661 ctttgaagac cttgctcaga tcccccagat cggcggccgc caccctcact ccttagcacg
     3721 gaacttggtc gagggccgag tttacccgtc ctgacgcctc ttgaagcgcc gccgcgacgg
     3781 tcgaagcgcc gttcggaggc gagggtgagg acgtctttga gggcgacggt gcgctcgccc
     3841 ttgtaccagc ccttccattc gcagttacgg caggaggcga aggtgcgctg ctcctcgggg
     3901 gcgagctgga tggtgatggc ggtgaggcgg cgaccggcgc agttcgggca ggctgacgct
     3961 gacatgggct tctcccgtgg gcttccgtgg gcttctcccg gcggagcggc tggcggctgg
     4021 cttggcgtag atctgctggc attgatcgga tgcgccggcc cataccttga ggccaacctc
     4081 ggggtcaagc accgtcaacc gtcgccacgg gcggccggag gccctatcgt ggtgtcatcc
     4141 caggccccct tccccgggcc gctcgttcga ggaggtcagg ctgccacgtc cccgtcacgc
     4201 gcgacggccg ccgcggccgc gcctgaccct gcccgaggtg cagcccgcgg gcaccgggga
     4261 gagcgccttc atgcggctgg tcgaggcggc ggtggcctcg atccccgagg agttccgcca
     4321 gcggctcgac aacgtcgagt tcgtggtcga ggagatcccc cgcggcgacg aggtccctgg
     4381 cgagggcatc cccctcggcc tctacctggg cgtcccgctg accgagcgga acgcgggcta
     4441 ctacggcgtg ctccccgacc ggatcgtgat cttccgccgc ccgatcgagg cgcgtgcccg
     4501 ctccgaggcg gccctggccg cgctggtgcg cgaggtcgtc atccatgagg tggcccacta
     4561 cttcgggatc gacgacgacc gcctcgacga gctgggctgg tagcccgttc gttcaggagg
     4621 cggcgggtcc ctggaccact ggaccgccgg ccgggacacc gccgaccacg gcgcccggga
     4681 tcgcctcgcc ctcgccgagg agcgccacca gcacgccagg ctcgccggct tcgacgtcga
     4741 gcgcgtcgac ctgcccgggg acgtcccgga ccacgacccg tcccggcggg agggtcagcg
     4801 cctgggtcgg tggtggcgcg gtggcgcccc tgccggtgcc agtcgcggcc ttgccgccgt
     4861 tccgcgtggc ggcttggccg gagggctggc cgccaaccgg gatcagctgc gcggtgacgg
     4921 cgctgtcgga ggtgttgacc aggacgacct tgccgccggt ggcgccgggg accagccagc
     4981 tgcggtcggg gagggcggtg ccgggatcga cataggcgac ctgctgcgcg ccagccaggg
     5041 cgctcgacac ccgcagggcg gcgatgtagt gggtgccgtc gcgtgagctg acctggaggc
     5101 cgaagctgcc gcccgaggcc ggtgaggacg cggtcgtcgg cgggttgatc gggatctgcg
     5161 ccagggcgcc gctcggaatg ggcttgtcaa aagccgggtc gtgcaggtcg aaggcaccct
     5221 tgtcgccgat gaccctggcg ctgacctgca gcgggctggt ggaggggttg gagaccagca
     5281 gggtggtctg gtggccgttg ccgctgaagc cgccggcgaa cgaccagctg gagcggggcg
     5341 tggactggcc gggcacgaag gtcgacggct ggccctgatg cgtgatgacc ccgccgacca
     5401 caaaccggcc ggtgcgggcc accgcctcca ccgagaacgg caggccttgg gcggcgctga
     5461 atttgaccgg cctcgccacg gtggagcggc gcggcacgaa gatctcgtca gtctcagcga
     5521 ccagggcgcc gttgggcagg tgcgggacca ggtcgaccac ggcgtcgtcg gcgtcgggat
     5581 tggcgatca
//
LOCUS       DASWDC010000094         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1439756, whole genome shotgun sequence.
ACCESSION   DASWDC010000094 DASWDC010000000
VERSION     DASWDC010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439756"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VF995_03130"
     CDS             complement(<1..651)
                     /locus_tag="VF995_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9342594.1"
                     /translation="MAMIRVAIIDDHPVARWGVEHLFQAHPEIEVVCAAPSVAEVEAA
                     GVAPDVVILDLYLEPGRPSLSAIAALAPRCRVLVMSASRTRGDILDAIRAGADGYLTK
                     QASDDAFIEGVRQVAAGGFYLSSQLADMLQADLDQDQAPGHNGSRPDWGQLAPREQET
                     LSCIAAGFTRGQTARRMGVSQSTVDTYVKRIRQKLGLGNKADLTRMAMELGHRDPPA"
     gene            complement(708..2048)
                     /locus_tag="VF995_03135"
     CDS             complement(708..2048)
                     /locus_tag="VF995_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9342595.1"
                     /translation="MQSPSRTGEAQASSERFLLLLFSLMRLAVLVQVTVASVVAWEHF
                     RHPVLLVVLLAGVWTESVLLVEAGRRRGALASERLLAVDIAAGMVSLWVLTALLKPSH
                     NPYVDIFFYPYTVVAMALVGIVVRRLSGVVLLTALMSGVYIGVMTARFGFGSTLLVNS
                     VTYWAYALVAWGVCGWYRRLSRQLDQARQEAVERGMELTSSRHAQELQELRMRAVEAA
                     LEHERERAGNFRDLHDRVLQTLEFLGRGGWVPDGRIRDRIAGEAVWLRQLIEGGLDGA
                     RGDLVAALGEVVERQAAAGLRVDLNAARIAADGLAVAGEVVDAVAGAVEEALGNVRKH
                     AGTNRAVVRAVPERGGVQVSVLDQGRGFDPATVRHGVGIPHSLTARVRQVGGAVRIDS
                     TPGTGTYVELWVPALAAAPAVAPAPASAPGPCPQNSGRAPVQPSTEQSADRQQP"
     gene            complement(2108..3256)
                     /locus_tag="VF995_03140"
     CDS             complement(2108..3256)
                     /locus_tag="VF995_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012835787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX9342596.1"
                     /translation="MARIVVFGAAGYTGRLTVEALTAAGQRPVLAGRGRERLAALADQ
                     HGGLEAAVADATDPASVRALVEPGDVLVSTVGPFTRYGEPAVAAAAGMGVHYVDSTGE
                     APFIRRVFAEFGPKAERSGAALLTAFGYDFVPGNLAGGLAIQDARNAAGGAADGAADG
                     AGAVPRRVEIGYFLTGDTSASGMSSGTRATIATGPGQRQHVWRDGRLVTEPIARRVRR
                     FADDGRQLPAVSFGGSEQLALPRWAPQLREVDVYLGWFGRRSRALQLASLLEPVALAL
                     PGARRRLQQRGERALGRTGQGPDAAARARTGALVIAVARDAAGRELARVRLAGGNGYD
                     LTGQLLAFAATRLAAGEVNGAGALGPVDAFGLEVLEAACRDAGLRRTA"
     gene            3389..4069
                     /locus_tag="VF995_03145"
     CDS             3389..4069
                     /locus_tag="VF995_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9342597.1"
                     /translation="MSKRKVSNQLALAVLAHLEERPMHPYEMATTMRERGKEHSIKLN
                     FGSLYTVVESLQRAGFITAQETVREGRRPERTVYEITEAGRAELYDWLCELLGEPAKE
                     YPRFMAGLSLLGVLPPAKGIAVLKQRERRLAELLTEGRQMLSGWIDGTRGTAPLPRVF
                     LIETEYELAMREAELNWLRGLLREIEGGTLSGVELWEQFHQLKAAGTSEPPATAPGEE
                     GAARTPPP"
BASE COUNT          620 a         1558 c         1510 g          512 t
ORIGIN      
        1 cgccggcggg tcgcgatggc cgagttccat cgccatcctg gtgaggtcgg ccttgttgcc
       61 gagccccagc ttctggcgga tgcgcttgac gtaggtgtcc accgtcgact ggctcacccc
      121 catccgccgt gccgtctgcc cgcgggtgaa ccccgccgcg atgcagctga gcgtctcctg
      181 ctccctcggg gcgagctgcc cccagtccgg ccggctcccg ttgtggcccg gcgcctggtc
      241 ctggtccagg tcggcctgga gcatgtcggc gagctgcgag gagaggtaga agccgccggc
      301 cgcgacctgg cggactccct cgatgaaggc gtcgtcgctg gcctgcttgg tgaggtagcc
      361 gtcggcgccg gcccggatcg cgtccaagat gtcgccgcgt gtccgggagg ccgacatcac
      421 cagcacccgg cagcgcggcg ccagcgccgc gatggcgctg agcgagggcc tgcccggctc
      481 caggtacagg tcgaggatca ccacgtccgg ggcgacgccg gccgcctcga cctccgccac
      541 cgagggcgcg gcgcagacga cctcgatctc cgggtgcgcc tggaacaggt gctcgacgcc
      601 ccagcgcgcc accgggtggt cgtcaataat cgcgacccga atcatcgcca tcggcacatc
      661 cctgattccc cccacggacc ggatgtcatg ccagcgccac gccttggtca tggctgctga
      721 cgatctgcag attgttccgt ggacggctgg accggcgcac gtcccgaatt ctggggacac
      781 ggccccggcg cggacgcggg tgcgggtgcg acagcgggtg cggcggcgag cgcgggcacc
      841 cacagctcga cataggtgcc ggtgccgggg gtgctgtcga tgcggaccgc cccgcccacc
      901 tgccgcacgc gggcggtcag cgaatggggg atcccgaccc cgtggcggac ggtggccggg
      961 tcgaagccgc ggccctggtc gagcacgctc acctgcaccc cgccgcgctc gggcacggct
     1021 cggaccaccg cccggttggt gcccgcgtgc ttgcggacgt tgccgagcgc ctcctcgacc
     1081 gcgccggcga ccgcgtccac cacctcgccg gcgacggcga ggccgtcagc ggcgatccgg
     1141 gcggcgttca ggtcgacccg cagccccgcc gcggcctgcc gctcgaccac ctccccgagg
     1201 gcggcgacca ggtcgccgcg ggcgccgtcg aggccgccct cgatgagctg gcgcagccac
     1261 accgcctcgc cggcgatgcg gtcgcggatg cgcccgtccg gcacccagcc gccccgcccc
     1321 agaaactcga gcgtctgcag cacgcggtcg tggaggtcgc ggaagttgcc ggcccgctcc
     1381 cgctcgtgct ccagcgcggc ctcgaccgcc cgcatgcgca actcctgcag ctcctgcgcg
     1441 tggcgcgagc tggtcagctc catgccgcgc tcgaccgcct cctggcgggc ctggtcgagc
     1501 tgccggctga ggcgccggta ccagccgcac accccccagg cgaccagggc atatgcccaa
     1561 taggtcaccg agttgaccag cagggtgctg ccaaacccga agcgggccgt catcaccccg
     1621 atgtagacgc cgctcatcag cgcggtgagc agcaccacgc cggaaaggcg gcggaccacg
     1681 atcccgacca gcgccatggc gacgacggtg tagggataga agaagatgtc cacgtagggg
     1741 ttgtgggagg gcttgagcag cgcggtcagg acccacagcg acaccatgcc ggcggcgatg
     1801 tcgacggcca gcagccgctc gctggccagc gcgccccggc gccgcccggc ctccaccagc
     1861 agcacgctct ccgtccagac gccagccagc agcaccacca gcaggaccgg gtgccggaag
     1921 tgctcccagg cgaccacgct ggcgaccgtg acctggacca ggacggccaa ccgcatcaac
     1981 gagaagagca gcagcaggaa ccgctcgctg ctcgcctggg cttcaccggt ccggctgggg
     2041 gactgcatcg ggccaccacc acgtcacgcc aaaaggggca acggcggtag tgccggattc
     2101 tactagatca agcagttctt ctcagaccgg cgtctcggca ggcggcctcc aggacctcca
     2161 ggccgaaggc gtcgaccggg ccgagggcgc cggcgccgtt cacctcgccg gcggcgaggc
     2221 gggtggcggc gaaggcgagc agctggccgg tgaggtcgta gccgttgccg ccggccaggc
     2281 gcacgcgcgc cagctcgcgg ccggcggcgt cgcgggcgac cgcgatgacc agggcgccgg
     2341 tgcgggcccg cgcggcggcg tcggggccct ggccggtgcg gccgagcgcc cgctcgccgc
     2401 gctgctggag acggcggcgg gcgccgggca gggccagcgc caccggctcc agcagggagg
     2461 cgagctgcag ggcgcggctg cggcgcccga accagccgag gtagacgtcg acctcccgca
     2521 gctgtggcgc ccaccgtggc agggcgagct gctcgctgcc gccgaaggag acggcgggca
     2581 gctgccggcc gtcgtcggcg aagcggcgca cccgccgggc gatcggctcg gtcaccagcc
     2641 ggccgtcccg ccagacgtgc tggcgctgcc ctgggccggt ggcgatggtc gcgcgggtgc
     2701 cgctgctcat ccccgacgcg ctggtgtcgc cggtcaggaa gtagccgatc tcgacccggc
     2761 gcggcaccgc tccggcgccg tcagcggcgc cgtcagcggc gccgccggcg gcgttgcggg
     2821 cgtcttggat ggcgagcccg cccgccaggt tcccggggac gaaatcgtag ccgaaggcgg
     2881 tgagcagcgc ggcgccgctg cgctcggcct tggggccgaa ctcggcgaag acccggcgga
     2941 tgaagggcgc ctcgccggtg ctgtcgacgt agtggacgcc catgccggcc gcggcggcga
     3001 ccgctggctc gccgtagcgg gtgaacggcc cgaccgtcga gaccagcacg tccccgggtt
     3061 cgaccagcgc ccgcaccgag gccgggtcgg tcgcgtcggc gacggcggcc tccagcccgc
     3121 cgtgctggtc ggccagcgcg gcgagccgtt cccggccgcg cccggccagc accggccgct
     3181 gcccggcggc ggtcagcgcc tcgacggtga gccggccggt gtacccggcg gcgccgaaga
     3241 caacgatgcg agccatagat acctattgca cctgtcgagg agggctggaa acaagtactt
     3301 gcgcgcggat gatcgcctgc gtatactcga ctgcgagtat tagccaccga gcaacccgac
     3361 gctgaaccca cctgggagcc acctgagcat gagcaagcgg aaggtctcca accagctggc
     3421 gctggcggtc ctggcgcatc tcgaggagcg tcccatgcac ccctacgaga tggccaccac
     3481 catgcgcgag cgtggcaagg agcacagcat caagctcaac ttcggctccc tctacaccgt
     3541 cgtcgagtcg ctgcagcggg ccggcttcat caccgcgcag gagaccgtcc gcgagggccg
     3601 ccgccccgag cggaccgtct acgagatcac cgaagccggg cgcgccgaac tgtacgactg
     3661 gctgtgcgag ctgctcggcg agccagccaa ggagtacccg cggttcatgg ccgggctgag
     3721 cctgctgggc gtcctccccc ccgccaaggg catcgcggtc ctcaagcagc gcgaacggcg
     3781 cctcgccgag ttgctcacgg aaggccggca gatgctgtcc ggatggatcg acggcacgcg
     3841 cggcaccgcg cccctgccgc gcgtgttcct gatcgagacg gaatacgagc tggccatgcg
     3901 cgaggccgag ctgaactggc tgcgcgggct gctccgcgag atcgagggcg gcaccctcag
     3961 cggggtggag ctgtgggagc agttccacca gctcaaagcc gccggaacaa gcgaaccgcc
     4021 cgcaaccgcc ccaggagagg agggagccgc acggacgccg ccaccatgac gcgccacgac
     4081 ccccagcggg gtgttgccgc acaccgccag gggtcgtgat ccccaaatgc cccggtccgc
     4141 agagcgaacc cgacactcga gggctgcatc ccgagcatag tcaggtcgga ctccaaggac
//
LOCUS       DASWDC010000095         3495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_930527, whole genome shotgun sequence.
ACCESSION   DASWDC010000095 DASWDC010000000
VERSION     DASWDC010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930527"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VF995_03150"
     CDS             complement(<1..1080)
                     /locus_tag="VF995_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9342598.1"
                     /translation="MEQTLEDVLGRGDAADLALIVPGPGGPRLTYRQLRARVAELAQR
                     LAGMGVGRGDRVAIVFPNGAEAIVAFLAAATVATAAPLNPAYTETEFRFSLEDSGARV
                     LVTGPGQAAAARAAIPAGGLVVEAAFSDQNSLKVKAQTPRSNPVDNHSEAAPVPDSLA
                     LGGAPQGWGGDGAEPGVAAAGFTAEPSRLREVAAADGLSPDDVALVLHTSGTTSRPKR
                     VPLRHRNLAASVDNIVRSYQLGPGDVSLCAMPLFHVHGLVASALATFGAGGTVVAPAL
                     DHPLGLLPLLASQRVTWFSGSPTLHRLLLRRAAGAAGDRARFPALRFVRSCSAALPLD
                     QFHEMQAAYGVPVLEAYGMTEASHQM"
     gene            complement(1125..2219)
                     /gene="mtnA"
                     /locus_tag="VF995_03155"
     CDS             complement(1125..2219)
                     /gene="mtnA"
                     /locus_tag="VF995_03155"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX9342599.1"
                     /translation="MTRAQPQRTALDLVAERRSVAWDGGLLIIDQTLLPGRVEVLRLE
                     TVARVEEAIQRLRVRGAPAIGLCGAYGVVVGLMERERADLGQALAALAEAAERIGSAR
                     PTAVNLSWAVGRVRAAAERGRDPGEFRALALAEAERIRLENRDACRRLAEHGRAELEG
                     VARVLTHCNTGWLATAEVGSALGVVYQKAERGEPVAVLACETRPLLQGSRLTVWELLA
                     AGIDVTLLPDGAGPWALASGQADAVLVGADRIARNGDTANKIGTYAHALAARAAGVPF
                     YVAAPLATFDPRVASGAEITIELRAPAEVRDLPIPGAPGTTVAPAGASAWNPAFDVTP
                     AALISAFITERGVLRPPFERSIAAALGASS"
     gene            complement(2239..2733)
                     /locus_tag="VF995_03160"
     CDS             complement(2239..2733)
                     /locus_tag="VF995_03160"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEX9342600.1"
                     /translation="MGRTEAAIKVGGAWMSGQYPQVQTQTRPATSKPVVPAKAGTAQQ
                     VDRRARLVLRRLDPWSVLKFSLLFYSCLMLIGLLVLAVLWFVLTQMGFFDTISGFADK
                     FTLTVQFSAGQVFRWYVLLGVFGVVLWSVVTVLLTLLFNLVNDVTGGIEVVLAERERR
                     PPVR"
     gene            complement(2748..>3495)
                     /locus_tag="VF995_03165"
     CDS             complement(2748..>3495)
                     /locus_tag="VF995_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX9342601.1"
                     /translation="VYRVKVHELPEAARNSRGTYIANIPGATFQPDEKIAAVLDLDDY
                     DDGKFLVLGTKNGMVKKTALLEYDSPRVGLIAINLRDDDELIGVKLTSGDDDLLFVSR
                     KAMAVRFSEASVRPMGRQTSGVTAMKLRSNDQVLAMEVVRSGADLLVITDAGFGKRTP
                     LEQYARKGRAIQGVKTAELIEARGFLAGAMVVQEEDDVFLITDSGQVIRTRVNEIRRA
                     SRATQGVRIMRLGSNGTRVAAVAPVVKDEE"
BASE COUNT          502 a         1240 c         1288 g          465 t
ORIGIN      
        1 catctggtgg ctcgcctcgg tcatgccgta ggcctccagc accggcacgc cataggccgc
       61 ctgcatctcg tggaactggt cgagcgggag ggccgcgctg caggagcgca cgaaccgcag
      121 cgccgggaag cgggcgcgat ccccagcggc gccggccgcg cgccgcagca gcagccggtg
      181 cagcgtcggg gagcccgaga accaggtgac ccgctgcgac gccagcaacg gcagcagccc
      241 gagcgggtgg tccagcgccg gcgcgaccac cgtgccaccc gcgccgaagg tcgccagtgc
      301 ggaggcgacc agcccgtgca catggaacag cggcatcgcg cagagcgaca cgtcccccgg
      361 tccgagctgg tagctccgca cgatgttgtc aaccgacgcg gcgaggttcc ggtggcgcag
      421 cggcacccgc ttcggccggc tggtggtccc actggtgtgc aggacgagcg cgacgtcatc
      481 gggagacagg ccgtcagcgg cggcgacctc cctgaggcgg ctcggttcgg cggtgaagcc
      541 cgccgcggcg acgcccggtt ccgcgccgtc acccccccac ccctgggggg cccccccgag
      601 agccagacta tcaggaaccg gggctgcttc ggagtggttg tccacagggt ttgacctggg
      661 cgtttgggct ttgaccttga gcgaattctg gtcgctgaag gccgcctcga ccaccaggcc
      721 gccagcggga atggcggccc gcgcggcagc ggcctgcccg gggccggtga cgagcaccct
      781 ggcgccggag tcttccaggg agaagcggaa ctcggtttcg gtgtaggcgg ggttcagcgg
      841 ggccgcggtc gccacagtgg cggcggcgag gaacgccacg atcgcctcgg cgccgttggg
      901 gaagacgatc gcgacccggt cgccgcgccc aactcccatg ccggcgaggc gctgggccag
      961 ttccgccacc cgcgcccgca gctggcgata ggtgagccgc ggcccccccg ggccagggac
     1021 gatcagcgcg agatcagccg cgtcgccgcg gccgaggacg tcctccagcg tctgctccat
     1081 gacccgatgg tagcggcggc tggtagcggc ggcggcttgg ttgctcagct gctcgctccg
     1141 agcgcggcgg cgatcgaccg ctcgaagggc ggccgcagca cgccacgctc ggtgatgaag
     1201 gcgctgatca gcgcggcggg ggtgacgtcg aaggcggggt tccaggcgct ggcgcccgcc
     1261 ggcgccaccg tcgtgcccgg ggctccgggg atggggaggt cgcggacctc ggcgggggcg
     1321 cgcagctcga tggtgatctc cgcgccgctg gcgacgcgcg ggtcgaaggt ggccagcggc
     1381 gcggccacgt agaacgggac cccggcggcg cgggcggcga gggcgtgggc ataggtgccg
     1441 atcttgttgg cggtgtcgcc gttgcgggcg atgcggtcgg cgccgaccag gaccgcgtcg
     1501 gcctgcccgc tggccagcgc ccatgggccg gcgccgtcgg gcagcagggt cacgtcgatg
     1561 ccggcggcca gcagctccca cacggtgagg cggctgccct gcagcaacgg gcgggtctcg
     1621 caggcgagca cggcgacggg ctcgccccgt tcggccttct ggtagacgac gccgagcgcg
     1681 gagccgacct cggcggtggc gagccagccg gtgttgcagt gggtgagcac ccgcgcgacg
     1741 ccctccagct cggcccggcc gtgctcggcg aggcggcggc aggcgtcgcg gttctccagg
     1801 cggatccgct cggcctcggc cagcgccagc gcccggaact cgcccgggtc gcggccacgc
     1861 tcggcggccg cacggacccg gccgaccgcc catgacagat tgaccgcggt cgggcgggcg
     1921 ctcccgatgc gctcggcggc ctcggcgagc gcggcaagcg cctgccccag gtcggcgcgc
     1981 tcgcgctcca tcaagccgac caccacgccg taggcgccgc agagaccgat cgccggcgcg
     2041 ccgcgcaccc ggaggcgctg gatcgcctcc tcgacccgcg cgacggtctc caggcgcagg
     2101 acctcgacgc ggccaggcag gagggtctgg tcgatgatca gcaggccacc gtcccaggcg
     2161 accgagcggc gttcggcgac caggtcgagg gcggtccgct ggggctgggc cctggtcatc
     2221 ggccgcgatg gaggccggtc agcggacggg cgggcggcgc tcccgctcgg cgagcacgac
     2281 ctcgatgccg ccggtgacgt cgttcaccag gttgaagagc agggtgagca ggacggtcac
     2341 cacactccac aacaccaccc cgaacacgcc gagcaggacg taccagcgga acacctggcc
     2401 tgccgagaac tgcaccgtga gcgtgaactt gtcggcgaag ccgctgatcg tgtcgaagaa
     2461 gcccatctgg gtcagcacga accagagcac cgccaagacc agcagcccga tcagcatcag
     2521 gcaggagtag aacagcagcg agaacttcag caccgaccag gggtcgagcc gccggagcac
     2581 cagccgggcg cgccggtcga cctgctgggc ggtccccgcc ttggcgggca cgaccggctt
     2641 ggaggtggcc gggcgggtct gcgtctgcac ctgcgggtac tggccgctca tccacgcacc
     2701 gccgaccttg atcgccgcct cagtgcgccc cacgtgccgc tcatgtgcta ctcctcgtcc
     2761 ttgacgaccg gggccacggc cgccacccgg gtgccgttgg agcccagccg catgatccgg
     2821 acgccctgcg tggcacggct ggcgcgccgg atctcgttga cgcgggtgcg gatgacctgg
     2881 ccgctgtcgg tgatgaggaa gacgtcgtct tcttcctgca ccaccatcgc gcccgcgagg
     2941 aaccctctgg cctcgatcag ttcggcggtc ttgacaccct ggatggcgcg gcccttgcgg
     3001 gcgtactgct ccagcggggt gcgcttgccg aagccggcgt cggtgatgac cagcaggtcg
     3061 gcgccggagc ggaccacctc catcgccagg acctggtcgt tggagcgcag tttcatcgcg
     3121 gtgactccgc tggtctggcg gcccatcggg cgcacgctcg cctcgctgaa ccggaccgcc
     3181 atcgccttac gcgagacgaa cagcaggtcg tcgtcgccgg aggtgagctt gacgccgatg
     3241 agctcatcgt catctctcag gttgatcgcg atcaggccga cacggggcga gtcgtactcc
     3301 agcagagcgg tcttcttcac catcccattc ttggtaccaa gaacaaggaa cttaccatcg
     3361 tcgtagtcgt ccaggtcgag cacggccgcg atcttctcgt ccggctggaa ggtcgcccct
     3421 gggatgttgg cgatgtaggt gccgcgcgaa ttgcgcgccg cctcgggcag ctcgtgcacc
     3481 ttcacccggt agacg
//
LOCUS       DASWDC010000096         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1358830, whole genome shotgun sequence.
ACCESSION   DASWDC010000096 DASWDC010000000
VERSION     DASWDC010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358830"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VF995_03170"
     CDS             complement(<1..231)
                     /locus_tag="VF995_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX9342602.1"
                     /translation="MELPMAVSIREVGPRDGLQGEAPVPVEARVRLVDALSAAGVGRI
                     EVGSFVSPKAVPAMAGTDRVFAAIRRAPGVVYS"
     gene            complement(311..931)
                     /locus_tag="VF995_03175"
     CDS             complement(311..931)
                     /locus_tag="VF995_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX9342603.1"
                     /translation="MGFPRRLLAENEELILDLRPHWWQLVGPALVTVLIVVAVAVALL
                     LMPSADRSGVVGLAAGVAAVLLFLVLVLPRVVTWATTHFVLTSDRLIYRHGLIAKYSK
                     EIPLERLNDVTFSQSFFERLIGAGDLQIESAGEHGQSTFQDIRRPEAVQLEIYRQMEA
                     NQQRMTGGGRPPSALEDLERLAALRDRGVITEAEFQRKKQDLLGRM"
     gene            complement(991..1875)
                     /locus_tag="VF995_03180"
     CDS             complement(991..1875)
                     /locus_tag="VF995_03180"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX9342604.1"
                     /translation="MTGVPTGTSVKNVDELGGYEERLRAALPSFFSPLTVVAKTPSTM
                     AVAADLADAGAPEGTTVVAEEQTAGRGRLGRSWVAPPGSSLLVSVVLRPPLPVRSVWL
                     CAALAGVSLAEAVRAVAAADLPVGSPTNSPVGLKWPNDLLIGGRKAAGLLAETRMEGE
                     RLAGVILGMGVNVNQGLMEGVAGWAPEVASRATSVALAAGHPVDRAALLGAWGERFLA
                     GYRQLADGAPGAILAAYRDQLETIGQPVRATRLDAAPVDGTAVDLTPNGSLVVLTSSG
                     RRVEVSAADVEHLRLATG"
BASE COUNT          330 a          704 c          737 g          251 t
ORIGIN      
        1 cgaatacacc acccccggag cccgccggat cgccgcgaac acccgatcag tcccggccat
       61 cgccggcacc gccttcggcg acacgaacga ccccacctcg atccgcccca ccccagcggc
      121 gctgagggca tccaccagcc gcacccgcgc ctccaccggc accggcgcct ccccttgcag
      181 cccatcacga ggcccgacct cgcggatgct caccgccatt ggcagctcca tggacacctc
      241 ttttggaggg tacggaccag acgatcgtag cggcctcgcg actgaagccg gggaggctgg
      301 acgaaccggc ctacatgcgg ccgaggaggt cctgcttctt gcgctggaac tcggcttcgg
      361 tgatgacgcc gcggtcgcgg agggcggcga ggcgttcgag gtcctcgagg gccgaggggg
      421 ggcggccgcc accggtcatg cgctgctggt tggcctccat ctggcggtag atttcgagct
      481 ggacggcctc ggggcggcgg atgtcctgga aggtggactg gccgtgctcg ccggcggatt
      541 cgatctggag gtcgccggcg ccgatcaggc gctcgaagaa cgactgggag aaggtcacgt
      601 cgttgaggcg ctccaaggga atctccttgg agtacttggc gatcaggccg tggcggtaga
      661 tcagccggtc gctggtcagc acgaagtggg tggtggccca ggtcaccacc cgtggcagca
      721 ccagcaccag gaacaggagc acggcggcga caccggccgc cagcccgacg accccggagc
      781 ggtcggcgga cggcatgagc agcagcgcga ccgccacggc caccacgatc agcaccgtga
      841 ccagcgcggg accgaccagc tgccaccagt ggggccggag atcgaggatc agctcctcgt
      901 tctcggccaa gagccgacgc gggaacccca cctcgatagc ctccttgaga atgccacccg
      961 ggcggagcgt agcaactagg cgaagcctac tcaaccagtg gcaaggcgga gatgctcgac
     1021 atcggcggcg ctgacctcga cgcggcggcc gctgctggtg aggacgacca ggctgccgtt
     1081 gggggtgagg tcgacggcgg tgccgtccac cggggcggcg tccaggcggg tggcgcggac
     1141 tggctggccg atggtctcca gctggtcgcg gtaggcggcg agaatcgcgc cgggagcgcc
     1201 gtcggcgagc tgccggtagc cggcgaggaa gcgctcgccc caggcgccga gcagggcggc
     1261 ccggtcgacc gggtgaccgg cggccagcgc gacgctggtg gcgcggctcg ccacctcggg
     1321 tgcccacccc gccacgccct ccatcagacc ctggttgacg ttgacgccca tgccgaggat
     1381 gacgccggcg agccgctctc cctccatccg ggtctcggcg agcagcccgg cggccttgcg
     1441 gccaccgatg agcaggtcgt tcggccactt cagcccgacc gggctgttcg tagggctccc
     1501 gaccgggaga tccgcagcag caaccgcccg gacggcctca gccagcgaga ccccggcgag
     1561 cgcggcgcac agccagaccg accggaccgg gagcgggggg cgcagcacca ccgagaccag
     1621 cagggagctg cccggcgggg cgacccagct gcggccgagg cggccacggc cggcggtctg
     1681 ctcctcggcg accacggtgg tcccctcggg ggcgccggcg tcggccaggt cggcggcgac
     1741 cgccatggtg gatggggttt tcgcgaccac ggtcagtggg gagaagaacg acggcagggc
     1801 agcgcgaagc cgctcctcgt agccgcccag ctcatccaca ttcttcacag atgttcccgt
     1861 cgggacgccg gtcatgcggt aaccgtacat agaatgcctt ggtctatggg ccgactgggc
     1921 gatggggcgg gagggtcgag gtggcaccac cacagccgaa gacgatgaag gagcggatcg
     1981 acgagctccg caagcgccgg gagcaggagc tgcacgccgg ct
//
LOCUS       DASWDC010000097         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_692307, whole genome shotgun sequence.
ACCESSION   DASWDC010000097 DASWDC010000000
VERSION     DASWDC010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692307"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VF995_03185"
     CDS             complement(<1..411)
                     /locus_tag="VF995_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9342605.1"
                     /translation="MTPAEPGPEPVLEYRRATIAYDRVSVVEAVDGVVTAGSSVALIG
                     PNGAGKSTLIKATLGLVPVASGSIRVLGRPPAATRREVAYVPQADTLDTEFPVSAAQV
                     VLMGRYRRIGWLRGAGRSDRAIAAAALHAVGLGER"
     gene            complement(413..1336)
                     /locus_tag="VF995_03190"
     CDS             complement(413..1336)
                     /locus_tag="VF995_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX9342606.1"
                     /translation="MLITALAALIVAAGCSSSTSTGPGAGTGGGKVLKVVATTTQVQD
                     FARVIGGDRIKLTGVLKPNVDPHDYEPSPADIDAIAQADLVVKNGVGLEKWFDDAVRS
                     AGFTGTVVDASQGVKIRSGNGTAEEKTGDPHIWHNPRNAKVMSANIERAFAAADPAGA
                     AAFKANLDAYDAKLTSLDADIARQVSGLANKKLVTNHDAFGYYVDRYGLEFVGSIIPS
                     FDTSAELSGKDVADLVAKIKETGVKAIFSESSLPPKTADTIGREAHVKVVEGENALYG
                     DTLGPPGSAGATYLQMEEHNTRTIVSALGGV"
     gene            1579..1863
                     /locus_tag="VF995_03195"
     CDS             1579..1863
                     /locus_tag="VF995_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342607.1"
                     /translation="MHPQLTSELVALRRSEFLHQAEQARKIAQSRSPGAAASPATRTP
                     WRSAAGFVLVRLGMRLAGRAFDNPVVVVRRLHDGEQAAVAVIWNKLDKAA"
     gene            1971..2588
                     /locus_tag="VF995_03200"
     CDS             1971..2588
                     /locus_tag="VF995_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEX9342608.1"
                     /translation="MQAGVGMRADSWQRFAGLGRAWGWVLFFGVLTLAVGLAAIVWPG
                     RTVVVIAVLFGVQLLVWGVFRLIQALALGEASGGARVLYALIGILSIIVGMLCLRHVL
                     QTIAVLALLLGVYWLVSGVVETWTALAHSDLPHRGMSIVMGVLGLIAGIVVLAAPGIS
                     LLTLTIVLGCWLMLLGISEVVAALRLRRLKEMSGNHRDYGGVQPA"
     gene            complement(2621..>3590)
                     /locus_tag="VF995_03205"
     CDS             complement(2621..>3590)
                     /locus_tag="VF995_03205"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEX9342609.1"
                     /translation="TPAERHPTCGELAAARQALQPQPQPQPRGQTWPLPPSQVQTKVQ
                     AERQAELQAQVGGPPRTPTQRLPGPATGTGTGTGPVPTLGPWHYQSLRAPSARLRVAL
                     WLTAAAGVASGLTNLGDTTLAHLFREGHWDTARLPLATSTTQVLLFLVTAYLFLVWVR
                     RAYDNLVAFGGRQLRFAPVWASVGWFVPVLGLFRPKQIVDDLWRASDPALPLRDELEG
                     AGGVGGTAGRWWQRPVPPLLTWWWYAFLASVLARATWFATVRAFASLLTLGLSSVLLE
                     DLRPSAGVAAVADLLTVAAALLAIGVVNEVTARQEARAVTVWPKRR"
BASE COUNT          491 a         1246 c         1308 g          545 t
ORIGIN      
        1 tcgctcgccc agcccgacgg cgtgaagcgc cgcggcggcg atcgcgcggt cggatcggcc
       61 ggcgccgcgg agccagccga tgcggcggta gcggcccatc agcaccacct gcgcggcgct
      121 gaccgggaac tcggtgtcga gggtgtcggc ctgcggcacg taggcgacct cgcgccgggt
      181 ggcggccggc ggccggccga gcacccggat cgacccgctg gccaccggga ccaggccgag
      241 agtcgccttg atcaaggtgg acttgccggc gccgttgggg ccgatgagcg cgaccgagct
      301 gcccgccgtg accacgccgt cgaccgcctc caccacggag acgcggtcgt aggcgatcgt
      361 ggcccgccgg tactcgagca ccggctccgg tcccggctcc gcgggcgtca cctcagaccc
      421 caccgagggc gctcacgatc gtcctggtgt tgtgctcctc catctggagg taggtggcgc
      481 ccgcggagcc gggcgggccg agcgtgtcgc cgtacagggc gttctcgcct tcgaccacct
      541 tgacgtgggc ctcgcggccg atggtgtcgg cggtcttggg cggcagggag ctctccgaga
      601 agatcgcctt cacgccggtc tccttgatct tggcgacgag gtcggcgacg tccttgccgg
      661 acagttcggc ggaggtgtcg aagctcggga tgatcgagcc gacgaactcg agcccatagc
      721 gatcgacata gtagccaaag gcgtcgtggt tggtgaccag cttcttgttg gcgagcccgc
      781 tgacctgccg ggcgatgtcg gcgtcgaggc tggtcagctt ggcgtcgtag gcgtcgaggt
      841 tggccttgaa ggcggcggcg ccggccgggt cggcggcggc gaaggcccgc tcgatgttgg
      901 cggacatgac cttggcgttg cgcgggttgt gccagatgtg cggatcgccg gtcttctcct
      961 cggcggtgcc gttaccggag cggatcttga cgccctggct cgcgtccacc acggttccgg
     1021 tgaagcccgc gctcctgacc gcgtcgtcga accatttctc caggccgacg ccgttcttga
     1081 ccaccaggtc ggcctgggcg atcgcgtcga tgtcggccgg ggagggctcg tagtcgtggg
     1141 gatcgacgtt cggcttgagc acgccggtca gcttgatgcg gtcgccgccg atcacccgcg
     1201 cgaagtcctg cacctgggtg gtggtggcca ccaccttgag caccttgccg ccaccggttc
     1261 cggcgccggg accggtgctc gtggaactgg agcagcccgc cgccacgatc agggcggcga
     1321 gggcggtgat gagcatgacc aggtgacggt tgcgcacgtt agctcccaga agcagcggtc
     1381 tcgaaccagc acctacatga aaatggttat cagattcgtt gtcaactggc gcgatcacga
     1441 gcagagcgac cagcggaccc ggcaggacac caccgagggc cggtccgctg tcggcaaatt
     1501 cggtttggaa tgtcacaggc agatgctagt ttcgctgtag acgagaatga tttggccgga
     1561 tattcggcgg aggcccgcat gcacccacag ttgacttcgg aactggttgc gctacggcgc
     1621 agcgaattcc tgcaccaggc cgagcaggcc cgcaagatcg cccagagccg ctcgcccggc
     1681 gccgcggcgt cacccgcgac ccgcaccccg tggcgctccg ccgccgggtt cgtgctggta
     1741 cggctcggta tgcgcctggc tggacgcgcg ttcgacaacc cggtggtggt ggtccggcgc
     1801 ctgcacgacg gcgagcaggc cgcagtcgcg gtcatctgga acaagctgga caaggccgcg
     1861 taaagctacg ggaacccgcg ccgcgtgcgc ggatctgggt ctaggcatcg ccatcccgct
     1921 ccgacaacgg ggaagaagag caacatctcc aacccttgag ggagcggaac atgcaagctg
     1981 gcgttggcat gcgggctgat tcctggcagc ggttcgccgg gctcggccgc gcctggggct
     2041 gggtcctctt cttcggggtc ctgaccctgg ccgtcgggct ggcggcgatc gtctggccag
     2101 gccggacggt cgtggtgatc gccgtcctgt tcggggtcca gctgctggtc tggggcgtct
     2161 tccgcctgat ccaggcgctc gccctcggcg aggccagcgg gggcgcccgg gtgctgtacg
     2221 ccctgatcgg catcctgtcg atcatcgtcg ggatgctgtg cctgcggcac gtcctccaga
     2281 cgatcgcagt gctcgcgctg ctgctggggg tctactggct ggtcagcggc gtggtggaga
     2341 cctggaccgc gctggcgcac agcgacctgc cgcaccgggg catgtcgatc gtcatggggg
     2401 tgctcggcct gatcgccggg atcgtggtgc tcgccgcgcc gggcatctcg ctgctgaccc
     2461 tcacgatcgt gctgggatgc tggctgatgc tgctcggcat ctccgaggtg gtcgccgcgc
     2521 tccgcctgcg ccgcctcaag gagatgagcg gcaaccaccg cgactacggc ggcgtgcagc
     2581 ccgcctgacg cgatgcgccg acgtggcgtg ccgcggctgc tcagcgccgc ttcggccaga
     2641 cggtcacggc ccgcgcttcc tggcgggccg tgacctcgtt caccaccccg atcgccagca
     2701 gcgcggcggc caccgtcagc aggtcggcga cggccgccac ccccgcggag ggccgcaggt
     2761 cctccagcag caccgacgac agccccagcg tcagcagcga ggcgaacgcc cgcaccgtgg
     2821 cgaaccaggt cgcccgcgcc agcaccgacg ccaggaacgc gtaccaccac caggtgagca
     2881 gcggtggcac cggccgctgc caccagcgcc cggccgtgcc cccgaccccg cccgcgccct
     2941 ccagctcgtc gcgtagcggc agggcggggt cgctggcgcg ccagaggtcg tcgacgatct
     3001 gcttggggcg aaacaatccg agcacgggca cgaaccagcc gacgctcgcc cataccggcg
     3061 cgaagcggag ctggcgcccg ccaaacgcca ccaggttgtc ataggcgcgc cggacccaca
     3121 ccaggaacag gtaggcggtc accaggaaga gcagcacctg cgtggtgctg gtggccagcg
     3181 gcagccgcgc ggtgtcccag tgcccctcgc ggaacaggtg cgcgagggtg gtgtcgccga
     3241 ggttggtgag cccgctggcg accccggccg ccgccgtcag ccacagggcg acccgcagcc
     3301 gtgccgaagg cgcccgcaac gactggtagt gccacggccc gagcgtcggc accggcccgg
     3361 tccccgtccc tgtccccgtg gcagggcccg gcaggcgctg cgtcggcgtc cgcggcggtc
     3421 cccccacctg ggcttggagc tcggcctggc gctcggcctg gaccttggtc tggacctgac
     3481 tcggcggcaa cggccaggtc tgaccccgcg gctgcggctg gggctggggc tgcagggctt
     3541 ggcgggcggc ggccagctcc ccgcaggtgg ggtggcgctc ggccggggtc
//
LOCUS       DASWDC010000098         3902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_931527, whole genome shotgun sequence.
ACCESSION   DASWDC010000098 DASWDC010000000
VERSION     DASWDC010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931527"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VF995_03210"
     CDS             complement(<1..310)
                     /locus_tag="VF995_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342610.1"
                     /translation="MRARRPLQWLRLRLVWLRTPLRLVHFPGIAVAVVAAAVVLAGTG
                     TASRLFLASTGEAAFKRAHDPRQEVMRFAGYESPYGAKAGEDPLRDEESGTDPIVQPL
                     V"
     gene            493..714
                     /locus_tag="VF995_03215"
     CDS             493..714
                     /locus_tag="VF995_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEX9342611.1"
                     /translation="MRTLYLRGVPDDVVERLERLAANEGTSVNAVAVRELAEISRRAD
                     NPALLGALPNLDIAPASIVADLDTERGTR"
     gene            711..1094
                     /locus_tag="VF995_03220"
     CDS             711..1094
                     /locus_tag="VF995_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9342612.1"
                     /translation="MIVVDASAALAALLNAGPARRTLGAEQLHVPHLIDAEVASGLRR
                     KVAGGEIGAGAGWTALDTWRHLGMVRYPVFGILDRVWQLRDNLSAYDACYVALAEQLE
                     CPLLTADRRLAREPGIACLITVVPR"
     gene            complement(1150..2148)
                     /locus_tag="VF995_03225"
     CDS             complement(1150..2148)
                     /locus_tag="VF995_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9342613.1"
                     /translation="MARPLAEIREQLAGIEADVRAGIERVEAALRDAVATADPFVQEA
                     AGYLVRAGGKRFRPTLVLLSAHLGDPTRPKVIGAAAAIELTHLSTLYHDDVMDEAEVR
                     RGARSANARYDNKIAILTGDYLFARASELTADLGTEPTRILAHAITRLVQGQIRDVRG
                     PLEGQDPVDHYLGVLTDKTGALIAAACRLGAQLAGAPDGIVKALTEYGERIGVAFQLG
                     DDLLDITAETSVAGKQPGTDLRAGLRTLPVLYLLRAGGPDADRLATLLHHPDQPGSAT
                     VEEALALLRGSPALAEARKAAEAEVARAKAALTPLPEGPARLALEAVADQVFDRDV"
     gene            complement(2151..3659)
                     /gene="nuoN"
                     /locus_tag="VF995_03230"
     CDS             complement(2151..3659)
                     /gene="nuoN"
                     /locus_tag="VF995_03230"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX9342614.1"
                     /translation="MHLLAQTAAAETPLAFPTVAWRAIAPELALAVAGVVLLLVVAWA
                     PNARRGFLATLTLLGIAVAAAFTAWNWSHASVAFDGAISLDGITRYARLILLAVGALA
                     TLMAAQDGARDKEAPPEVFPLLLFALTGMILLAAATDLLVVFIALEILSLALYIMAGA
                     TRRARAQEAAMKYFLLGAFSSAFLLYGIALCYGATGTTSLLGIARASADPTIHSALLL
                     AGMAMLAVGFCFKVAAVPFHMWTPDVYQGAPIPVTAFMAAGTKAAGFAVFLRVFAGAL
                     GGLVLDWRPVISAVAVVTMLVGAVLALVQSDLKRMLAYSSISHAGYLLIGLVAGATVP
                     EGVSASMFYLLAYAFTVMGAFMVVQWTSRTAEGAGEERSSLEDYRGFGRRHPWHGALL
                     VLFMLSLAGIPPLSGFFAKYYVFQAGIDAGLTWLVVVGVVSSVIAAFFYLRVIVLAFL
                     QEPAEAAADPAAGERAPVLGVALAIAAVLTVAIGLAPQVLVSAAQTAGKILG"
     gene            complement(3689..>3902)
                     /locus_tag="VF995_03235"
     CDS             complement(3689..>3902)
                     /locus_tag="VF995_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342615.1"
                     /translation="VYMLWAYQRMWQGQITDDENAVIADLTVREWVVVIPLLAAIIAL
                     GMFPRPLLQRVEPSARQVTQSVQSIR"
BASE COUNT          592 a         1359 c         1436 g          515 t
ORIGIN      
        1 ggaccagagg ctgaacgatc gggtcggtgc cgctctcctc gtcccgcagt gggtcctcac
       61 cggccttcgc cccgtagggg gactcgtagc ccgcgaagcg catgacctcc tggcgcgggt
      121 cgtgcgcccg cttgaacgcg gcctctcccg tcgaggcgag aaacagccgg ctcgcggtcc
      181 cggtccccgc cagcaccacc gccgcggcca ccaccgccac cgcgatccca gggaaatgga
      241 cgagccgcag cggcgtccgc agccacacca gccgcagccg caaccattgc aatggccgtc
      301 gcgctctcat gtcccaccac cgcgctccgg cgctacatta tcggcgccat ccaggccacg
      361 caggagtacc cgggtcctgt gggacaattc tcgcatgccc cttcgtgccc aaccaggcct
      421 ggtttgcttg gtcaacagta ccaggacgca cgacctagca gttttgcaag caacttgcta
      481 gcattctgcg acatgagaac gctctatctg cgtggcgtgc ctgacgacgt cgtcgagcgc
      541 ctcgaacggc tggccgccaa cgagggaacc tccgtcaatg ccgtggcggt ccgcgaactg
      601 gcggagatct cccgccgggc cgacaacccc gcgctgcttg gcgccctgcc caacctggac
      661 atcgcgccag cgtcgatcgt ggcggacctg gataccgagc gcggcacgcg atgatcgtgg
      721 tcgacgcctc cgccgccctc gcggcgctgc tgaacgccgg ccccgcacgc cgaacccttg
      781 gcgcggagca actccacgtc ccccacctca tcgacgcgga ggttgccagc ggcctgcgac
      841 gcaaggtcgc tggcggggag atcggcgcgg gcgccggctg gaccgccctc gacacctggc
      901 ggcacctggg catggtccgc tatccggtct ttgggatcct ggaccgtgtc tggcagctcc
      961 gggacaacct ctcggcttac gacgcctgct acgtcgctct cgctgagcag ctggagtgcc
     1021 cgctgctgac cgccgaccgg cgcctcgcgc gggaaccggg gatcgcctgc ctcatcaccg
     1081 tggttcctcg ttagcgtctg cctggcgatt cgacgcggta gcggcgcacg ggccgcgcca
     1141 tccagaattt caaacgtcgc ggtcgaagac ctggtcggcg acggcctcca gggcgaggcg
     1201 ggcggggcct tcggggagcg gggtgagggc ggccttggcg cgggcgacct ccgcttcggc
     1261 cgccttgcgg gcctcggcga gcgccgggga gccgcgcagg agggcgagcg cttcctcgac
     1321 ggtggcgctg ccaggctggt cggggtggtg gagcagggtg gcgaggcggt cggcgtcggg
     1381 cccgcccgcg cgcagcaggt agaggaccgg gagggtgcgg aggccggcgc ggaggtcggt
     1441 gccgggctgc ttgccggcga cgctggtctc ggcggtgatg tcgaggaggt cgtcgccgag
     1501 ctggaaggcg acgccgatcc gttcgccgta ctcggtcagc gccttgacga tcccgtcggg
     1561 ggcgccggcg agctgggcgc cgaggcggca ggcggcggcg atcagcgcgc cggtcttgtc
     1621 ggtcagcacg ccgaggtagt ggtcgaccgg gtcctggccc tccagggggc cgcggacgtc
     1681 gcggatctgg ccctggacca ggcgggtgat cgcgtgggcg aggatccggg tcggctcggt
     1741 gccgaggtcg gcggtgagct cggaggcgcg ggcgaagagg tagtcgccgg tgaggatggc
     1801 gatcttgttg tcgtagcggg cgttggcgga gcgggcgccg cggcgcacct cggcctcgtc
     1861 catgacgtcg tcgtggtaga gggtcgacag gtgggtcagc tcgatcgcgg cggcggcgcc
     1921 gatcaccttg ggcctcgtgg ggtcgccgag gtgggcgctg agcagcacca gcgtcgggcg
     1981 gaaacgcttg cccccagcgc ggacgaggta gccggcggcc tcctggacga acgggtcggc
     2041 ggtggcgaca gcgtcgcgca gggcagcctc gacccgctcg atgcccgcgc ggacgtcggc
     2101 ctcgatcccg gcgagttgct ccctgatctc cgcgagcggc cgcgccatgc ctagcccagg
     2161 atctttccgg ccgtttgcgc ggcgctgacc agcacctgcg gtgcgaggcc gatggcgacg
     2221 gtcagcaccg cggcgatcgc cagcgccacc ccgaggacgg gggcgcgctc gccggcggcc
     2281 gggtcggcgg cggcctcggc gggctcctgc aggaaggcga gcacgatcac gcgcaggtag
     2341 aagaacgcgg cgatcaccga gctgaccacc ccgacgacca ccagccaggt gagcccggcg
     2401 tcgatgccgg cctggaacac gtagtacttg gcgaagaacc ccgacagcgg cgggatcccg
     2461 gccagcgaca gcatgaacaa gaccagcagc gccccgtgcc atgggtggcg ccgaccgaag
     2521 ccgcggtagt cctcaagcga cgagcgctcc tcgcccgcac cctcggccgt ccggctggtc
     2581 cactgcacca ccatgaacgc gcccatcacg gtgaaggcgt aggcgagcag gtagaacatg
     2641 ctggccgaga cgccctccgg gacggtcgcg ccggccacca ggccgatcag caggtagccg
     2701 gcgtgggaga tcgaggagta ggccagcatc cgcttgaggt ccgactggac gagggcgagc
     2761 accgccccga ccagcatggt gaccaccgcg acggcgctga tcaccggtcg ccagtcgagc
     2821 accagcccgc cgagcgcccc ggcgaagacc cgcaggaaca ccgcgaaccc ggccgccttg
     2881 gtcccggcgg ccatgaaggc ggtcaccggg atcggcgcgc cctggtagac gtccggggtc
     2941 cacatgtgga acggcaccgc cgcgaccttg aagcagaacc cgaccgccag catcgccatc
     3001 ccggcgagca gcagcgccga gtggatggtc gggtcggcgc tggcgcgggc gatcccgagc
     3061 aggctggtgg tcccggtcgc gccgtagcac agcgcgatcc cgtacagcag gaacgcggag
     3121 gagaaggcgc ccagcaggaa gtacttcatg gccgcctcct gcgcccgcgc ccggcgggtc
     3181 gcgccggcca tgatgtagag ggcgagcgag aggatctcca gcgcgatgaa caccaccagc
     3241 aggtcggtgg cggcggccag caggatcatc ccggtgagcg cgaacagcag cagcgggaac
     3301 acctcgggcg gcgcttcctt gtccctcgcg ccgtcctgcg cggccatcag ggttgccagc
     3361 gcgcccaccg ccagcaggat cagccgtgcg tagcgggtga tcccgtcgag ggagatggcg
     3421 ccgtcgaagg cgaccgaggc gtggctccag ttccaggcgg tgaaggcggc ggcgacggcg
     3481 atcccaagca gcgtgagggt ggcgagaaag ccgcgccggg cgttcggcgc ccaggcgacc
     3541 accagcagca gcaccacccc ggcgacggcg agggccaact ccggggcgat cgcgcgccag
     3601 gcgaccgttg ggaaagccaa cggcgtctcg gcggcggcgg tctgggccag caggtgcacg
     3661 gttccgctct ccctccctat gacttgcgct agcggatcga ctggacggac tgggtgactt
     3721 gccgagcgga cggctcgacc cgctgcagca gcggccgggg aaacatgccg agcgcgatga
     3781 tcgcggccag cagcgggatc accacgaccc attcgcgcac ggtcaggtcg gcgatgacgg
     3841 cgttctcgtc gtcggtgatc tgcccctgcc acatgcgctg gtaggcccac agcatgtaga
     3901 cg
//
LOCUS       DASWDC010000099         3138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_346922, whole genome shotgun sequence.
ACCESSION   DASWDC010000099 DASWDC010000000
VERSION     DASWDC010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346922"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /gene="lnt"
                     /locus_tag="VF995_03240"
     CDS             complement(<1..1397)
                     /gene="lnt"
                     /locus_tag="VF995_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX9342616.1"
                     /translation="MSRLRLTGPAPRVAAALLTGVALALAFPGADLGSVAFVALVPLL
                     LAVETAGPRRAGALGYLAGLTFFGLHLAWIEAFLSWTGPVAWLAWGALSAVEALFVAA
                     FFALVPATRRLGDWRVVLLPACWATLELIRAHVPIGGFPWGLLGVSQHHGGPLLPLAR
                     VVGVYGLSAVLVAVNLALARALRALGRGFAAQRQPHSAGVVRSRRWVAPAVAWPLLAA
                     AFAVSGLLAPAPPPVSGPPVDVAVVQGNVPGAARSTRADRGATTEQVFANHVRDTEAL
                     ANRAGPRPQLIIWGEGAVDDDPFADADRLTRIAQAASAVGAPLLVGASDNVGTDHLAT
                     DALLFSPDGRLRDRYRKRRLVPFGEYVPWGGFLGVLIPATREGVPTDKVPGRSLATMT
                     APGVAVRFGTLICWESTYAEDARTLARDGAGFLVVITNNASFGVSAGSPQHLAAGQLR
                     AVEEGRTVVQAAVSGP"
     gene            complement(1379..1642)
                     /locus_tag="VF995_03245"
     CDS             complement(1379..1642)
                     /locus_tag="VF995_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HEX9342617.1"
                     /translation="MPEDEEDEAQVEASLDELIAKKADRPTPVDEDEDEVLLALSRDE
                     ARESLTTKVLPVQQNEFVCKSCFLVKHRSQLADRRRQLCRDCA"
     gene            complement(1818..>3138)
                     /locus_tag="VF995_03250"
     CDS             complement(1818..>3138)
                     /locus_tag="VF995_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342618.1"
                     /translation="ERELTAARERHAEASELLGACAARLEAATAAAERHASLDAKAPC
                     PTCGQPLGTAHAEVARAHARELAAARAAHELASAERDTVTETGKALAARREALQQDLK
                     AAREAEQRAARAVIRAEEAERAIGVAADEVAARAAELAAIPDPGFDPDAHTAAKQAAE
                     ALQRARLAFGELRGRAERADTERALAKEALARADQADSQAANLDADLAAPALVGAERA
                     LATARAAEAAATQAARHTHGAQQRTAEALAGARRLVEQRATELAAARARHDEVAALEE
                     HSAYLARLVDLVAGFRLHLVSRLGRRLSVEAAGLFAELTDHEYQDLVVDPEDYSVRIA
                     DNGAEYELARFSGSENDLASLSLRVAVSLVIAEGAGELGLLVLDEVLGSLDRERRERM
                     LSALTTLQGRFRQVLLVTHNDEVKDMLPAAIEVRKGPHRTSTATVLA"
BASE COUNT          395 a         1104 c         1249 g          390 t
ORIGIN      
        1 gggccggaga cagcggcctg gacgacggtg cggccctcct cgacggcgcg gagctgcccg
       61 gcggccaggt gctgcggcga gccggcgctg acgccgaagg aggcgttgtt ggtgatcacc
      121 acgaggaagc cggcgccgtc gcgcgcgagg gtgcgggcgt cctcggcgta ggtggactcc
      181 cagcagatca gggtgccgaa gcggaccgcg acgccggggg cggtcatggt cgccagcgag
      241 cggccgggga ccttgtcggt ggggacgccc tctcgggtgg cggggatgag cacgccgagg
      301 aacccacccc aggggacata ttcgccgaag gggaccaggc ggcgtttgcg gtagcggtcc
      361 cgcaggcgcc cgtcggggct gaacagcagc gcgtcggtgg cgaggtgatc tgtgccgacg
      421 ttgtcggagg cgccgaccag cagcggcgcg ccgacggcgc tggcggcctg ggcgatgcgg
      481 gtgagccggt cggcgtcggc gaaggggtcg tcgtcgaccg cgccctcccc ccagatgatc
      541 agctggggac gcggcccggc gcgattggcg agggcctcgg tgtcacggac gtgattggcg
      601 aacacctgct cggtggtggc gccgcggtcg gcgcgggtgc tgcgggcggc gccggggacg
      661 ttgccctgga cgacagccac gtcgaccggc gggccgctga ccggcggcgg cgccggggcg
      721 agcagccccg acaccgcgaa cgcggcggcc agcagcggcc aggcgaccgc cggcgcgacc
      781 cagcgccgcg agcggaccac cccggccgag tgcggctggc gctgagcggc gaacccgcgc
      841 ccgagcgccc gcagcgcccg tgccagggcg aggttgaccg cgaccaggac cgcgctgagg
      901 ccgtagacgc cgacgacgcg ggccagcggc agcagcgggc cgccgtggtg ctggctcacc
      961 ccgagcagcc cccaggggaa gccgccgatg gggacgtggg cgcggatcag ctccagggtc
     1021 gcccagcagg cgggcagcag caccacccgc cagtcgccga gccgccgggt cgccgggacc
     1081 agtgcgaaga acgcggcgac gaacagcgcc tcgaccgccg acaaggcgcc ccaggcgagc
     1141 caggcgaccg gcccggtcca ggacaggaat gcctcgatcc aggcgagatg cagcccgaag
     1201 aaggtgagtc cggccagata gccgagcgcc ccagcccgcc gcggcccggc cgtctcgacg
     1261 gcgagcagca gcgggaccag ggcgacgaag gccaccgacc caaggtcggc tcccgggaag
     1321 gcaagcgcga gcgcgacccc ggtgagcagc gcggcggcga cccgcggcgc cggtccggtc
     1381 aggcgcagtc gcgacacagc tggcgccggc ggtcggcgag ctggctgcga tgcttgacca
     1441 gaaagcagct cttgcagacg aactcgttct gctggaccgg cagcaccttg gtggtgaggg
     1501 actcgcgggc ctcgtcgcgg ctgagcgcga gcaggacctc gtcctcgtcc tcgtccaccg
     1561 gcgttggccg gtcggccttc ttggcgatca gctcgtcgag cgatgcttcg acctgagctt
     1621 cgtcctcttc atcctcaggc accattagtt cctttcattc cgttcgtgat gtcgggagcg
     1681 aggtgcgccg gcttatgtat cacgaactga cgtactacgt gccggtgctg tgtgcgtaac
     1741 agcgcgcgga ccctggttgt tcccggcgaa tccggcggga atcaccggcg caaactgtac
     1801 ggtcagcgcg gcgtctgtca ggcgagcacc gtcgccgtgg aggtccggtg ggggcctttg
     1861 cggacctcga tggctgcggg aagcatgtcc ttcacctcgt cgttgtgggt caccagcagg
     1921 acctggcgga agcggccctg cagggtcgtg agggcggaca gcatacgctc gcggcgctcc
     1981 cggtccagcg aaccgagcac ctcgtcgagc accagcagac cgagctcacc cgcaccctcg
     2041 gcgatcacca ggctgaccgc cacccgcaac gagaggctgg cgaggtcgtt ctcggagccg
     2101 gagaagcgtg ccagctcgta ttcggcgccg ttgtcggcga tccggacgga gtaatcctcc
     2161 ggatcgacca ccaggtcctg gtactcgtgg tcggtcagct cggcgaacag cccggccgcc
     2221 tcgaccgaca ggcgccgccc gagccgtgag acgaggtgga ggcggaaccc ggcgaccagg
     2281 tcgaccagcc gggcgaggta ggccgagtgc tcctcgaggg cggcgacctc gtcgtggcgg
     2341 gcgcgggcgg cggccagctc ggtggcgcgc tgctcgacga ggcgccgcgc gccggccagc
     2401 gcctcggcgg tgcgctgctg cgcgccgtgg gtgtggcggg ccgcctgggt ggccgcggct
     2461 tcggccgcgc gggcggtggc cagggcccgc tcggcaccga cgagggccgg tgcggcgagg
     2521 tccgcgtcga ggttggcggc ctggctgtcg gcctggtcgg cacgggcgag tgcctccttg
     2581 gccagcgccc gctcggtgtc ggcgcgttcc gcccggccgc gcagctcccc gaacgcgagc
     2641 cgggcccgct gcagcgcttc ggcggcctgc ttcgccgcgg tgtgggcgtc cgggtcgaag
     2701 ccggggtcgg ggatggcggc gagctccgcg gcgcgggcgg cgacctcgtc ggcggccacg
     2761 ccgatggccc gttccgcctc ctcggcgcgg atgaccgcgc gggcggcgcg ctgctcggcc
     2821 tctcgcgccg ccttcaggtc ctgctgcagg gcctcgcgcc gggcggccag ggccttgccc
     2881 gtctcggtga cggtgtcccg ctcggcgctg gccagctcgt gggcggcgcg ggcggcggcc
     2941 agctcccgcg cgtgggcgcg ggcgacctca gcgtgcgccg tgccgagcgg ctggccgcag
     3001 gtcgggcagg gcgcctttgc gtccaggctg gcgtggcgct cggccgcggc ggtggccgcc
     3061 tccaggcgcg cggcgcaggc gccgagcagc tcgctcgcct cggcgtgccg ctcgcgcgcg
     3121 gcggtcagct cccgctcg
//
LOCUS       DASWDC010000100         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_180167, whole genome shotgun sequence.
ACCESSION   DASWDC010000100 DASWDC010000000
VERSION     DASWDC010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180167"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..734)
                     /locus_tag="VF995_03255"
     CDS             complement(21..734)
                     /locus_tag="VF995_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX9342619.1"
                     /translation="MAAESREEAVAGTRVATTAVVRPGVRLGPGAVVGEWCLLGEPPA
                     GHPPSEDGNPLEGGALTVLGAGALLRSHTVIYAGNRIGDRFQSGHHVLVREDNEIGDD
                     VAIGSGTMVEHHVRVGHRVRLHGGVFVPELCVLEDDCWLGPRVCLTNAPYPRCPEVSA
                     CMRGVRVGRRAKLGANVTVLPGVTIGEDALIGAGAVVTDDVPAGAVVAGNPARTLGWA
                     GDLTCPVGLDHRPYPLERP"
     gene            complement(724..1740)
                     /locus_tag="VF995_03260"
     CDS             complement(724..1740)
                     /locus_tag="VF995_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9342620.1"
                     /translation="MRIALLGYGYWGPNLARNLHVGVGQEWVACADLDPGRRAEVARR
                     YPWVRVHADADAVVDDPEVDALVVATPGRSHAGLVRRALQAGKHVLVEKPLALNTAEA
                     VALAESAARAGLVLMVGHTFEYNPAVTKIRALLDAGELGELWYLHSQRVNLGRIQRDV
                     SALWSIGPHDVSIANYLVGSPPRWVSARGARYLHEEVEDVVFATLGYDGGVLAHLHVS
                     WLDPSKVRRTTVVGSRRMVVFDDLDSEAKLRVYDKGADPVEGGFGEYQFRVRSGDIHV
                     PRTDLAEPLALELEHFLDCCASGARPRTDAWNGVRVVAALEAAQQSLDKGGQQVEVVL
                     PGGR"
     gene            1922..3100
                     /locus_tag="VF995_03265"
     CDS             1922..3100
                     /locus_tag="VF995_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9342621.1"
                     /translation="MSEAFTLTEDQKLFRKEWRRLFEDKFAARAAEIDQKAEFPWDNV
                     EVLKSAGLLGLNIPEEYGGGGADALTHCLLVEEVARVCASTSLIPAVNKLGTLPILFA
                     GTDEQKRRFLTPIAEGDALISYCLTEPGSGSDAAAMASRAQRDGDDYVISGTKRFITG
                     GGVSKLYVWYAVTDPAKGGRGISAFVLDAAMPGFEIGKHEDKLGIRGSPTTEVICNDV
                     RVPAASRLGEEGAGFPLALRALQHSRITIGSQALGIAQGAFDFALNYVQERHQFNQPI
                     ADFQGIQWMLADMAMQLEAARALVYTAAAKADREDDDLTFFSAAAKCFASDMAMKVTN
                     DAVQLLGGYGYIRDFPAERMLRDARITAIYEGTNQIQRVVIARHLLGGLTAEARASKR
                     "
     gene            3180..>3585
                     /locus_tag="VF995_03270"
     CDS             3180..>3585
                     /locus_tag="VF995_03270"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9342622.1"
                     /translation="MGVAHANLMGNVHGGEIMKLIDTAAGIAASRHAGGPAVTVSLDQ
                     MSFLSPVRVGNVVFVHASVNDVGRTSMEVGVRVEAEDFVTGQRTHTYSAYLVFVALDE
                     QQRPRPVPPLEPETEVERRRQAEAKIRREFRLA"
BASE COUNT          554 a         1347 c         1205 g          479 t
ORIGIN      
        1 gccgtcaggc cgtcaggccg tcaggggcgc tccagcggat agggccggtg gtccaggccg
       61 accgggcagg tgaggtcgcc cgcccagccg agcgtccggg ccgggttgcc ggccaccacc
      121 gcgcccgccg gcacgtcgtc ggtcaccacc gcacccgcgc cgatcagggc gtcctcgccg
      181 atggtgacgc cgggcaggac ggtcacgttg gcgcccagct tggcgcggcg ccccacccgc
      241 acaccgcgca tgcaggcgct cacctcgggg cagcgggggt agggcgcgtt ggtcaggcag
      301 acgcgcgggc cgagccagca gtcatcctcc aacacgcaca gctccgggac gaacacgccg
      361 ccgtgcagcc gcacccggtg gccgacgcgg acgtggtgct ccaccatcgt gcccgagccg
      421 atcgcgacgt cgtcgccgat ctcgttgtcc tcgcggacca acacgtgatg gccgctctgg
      481 aagcggtcgc cgatgcggtt gcccgcgtag atcaccgtgt gcgagcgcag cagcgcaccc
      541 gcgccgagca cggtcagcgc cccgccctca agggggttgc cgtcctcgga tggagggtgc
      601 ccggcgggcg gctccccgag caggcaccac tctccaacga ccgcgcccgg cccgagccgg
      661 acccccggcc gcaccaccgc cgtggtcgcc acgcgggtcc ccgccacggc ctcctcccga
      721 gactcagcgg ccaccgggga gcaccacctc gacctgctgg ccgcccttgt ccagcgactg
      781 ctgcgcggcc tccagcgcgg ccacgacccg cacgccgttc caggcgtcgg tccgcggccg
      841 ggcgccgctg gcgcagcagt ccaggaaatg ctccagctcc agcgcgagcg gttcggccag
      901 gtcggtgcgc ggcacgtgga tgtcgccgga gcggacccgg aactggtatt cgccgaagcc
      961 gccctcgacc gggtcggcgc ccttgtcgta gacccgcagc ttggcttcgg agtccaggtc
     1021 gtcgaacacc accatgcggc gcgagccgac caccgtggtg cgccgcacct tcgacgggtc
     1081 cagccaggac acgtgcaggt gggccagcac cccgccgtcg tagccgaggg tggcgaacac
     1141 cacgtcctcg acctcctcat gcaggtagcg ggcccctcgc gccgagaccc agcgcggcgg
     1201 ggagccgacc aggtagttgg cgatcgagac gtcgtgcggc ccgatcgacc acagcgcgct
     1261 cacgtcccgc tggatccgcc cgaggttgac ccgctgcgaa tgcaggtacc acagctcgcc
     1321 cagctcgccc gcgtccagca gcgcccggat cttggtcacc gccgggttgt actcgaaggt
     1381 gtggcccacc atcagcacca gcccggcccg cgccgccgac tccgccagcg ccaccgcctc
     1441 cgccgtgttg agcgccagcg gcttctcgac cagcacgtgc ttgccggcct gaagcgcccg
     1501 ccgcaccagc cccgcgtgac tgcgccccgg cgtcgccacc accagcgcgt cgacctcggg
     1561 gtcgtcgacg accgcgtcgg cgtcggcgtg cacccgcacc cacgggtagc gccgcgccac
     1621 ctccgcccgc cgccccgggt cgaggtccgc gcaggcgacc cactcctgcc caaccccgac
     1681 atgcaggttc cgggccaggt tcggccccca gtagccgtag ccgagcagtg cgatcctcac
     1741 gtctgaaccc ctccggaggg acttgtccac aggggggcca tcctaccctg ggggttcggg
     1801 ggcatggtcc cgcggccgga aggaccgcag gccgatccca cggctgccgc gggcccccga
     1861 tggatcaagc cggtcactat cattggggag cgcgccgccg tacgagctga gaggggcaac
     1921 gatgtccgag gccttcaccc tcaccgagga ccagaaactg ttccgcaagg agtggcggcg
     1981 gctgttcgag gacaagttcg ccgcccgtgc cgccgagatc gaccagaagg cggagttccc
     2041 ctgggacaac gtcgaagtgc tcaagtccgc cgggctgctc ggcctcaaca tccccgagga
     2101 atacggcggc gggggcgccg acgcgctcac ccactgcctg ctggtggagg aggtcgcccg
     2161 ggtctgcgcc agcacctcgc tgatcccggc ggtcaacaag ctcggcaccc tgccgatcct
     2221 gttcgccggc accgacgagc agaagcgccg cttcctcacc ccgatcgccg agggcgacgc
     2281 gctcatctcc tactgcctca ccgagccggg gtcgggatcg gacgccgccg ccatggcctc
     2341 ccgcgcccag cgcgacggcg acgactacgt gatcagcggc accaagcgct tcatcaccgg
     2401 cggcggcgtc agcaagctct acgtctggta cgccgtcacc gacccggcca agggcgggcg
     2461 cggcatctcc gcgttcgtgc tcgacgccgc catgcccggc ttcgagatcg gcaagcacga
     2521 ggacaagctc ggcatccgcg gctcgcccac caccgaggtc atctgcaacg acgtccgcgt
     2581 ccccgccgcc agccgcctcg gcgaggaggg cgccggcttc cccctggccc tgcgcgccct
     2641 gcagcacagc cgcatcacca tcggctccca ggccctcggc atcgcccagg gcgccttcga
     2701 cttcgccctc aactacgtcc aggaacgcca ccagttcaac cagccgatcg ccgacttcca
     2761 gggcatccag tggatgctgg ccgacatggc catgcagctc gaagccgccc gcgctctggt
     2821 ctacaccgcc gccgcaaagg ccgaccgcga agacgacgac ctcaccttct tctccgctgc
     2881 cgccaaatgc ttcgcctccg acatggcgat gaaggttaca aacgacgcgg tacagctcct
     2941 tggtggctat gggtatattc gcgacttccc cgctgagaga atgctccgcg acgctcgcat
     3001 taccgccata tacgagggga caaatcaaat tcagcgggtc gttatcgcac gtcatttgct
     3061 tggcggcctg accgccgagg ccagagccag caagcggtag agggagagga gggacgctgt
     3121 ccgggagcca tgaagagcca aaggcgccgc ggcagtcggc ggtgacgttg tcgcaggcga
     3181 tgggagtggc gcacgccaac ctgatgggga atgtgcatgg cggggagatc atgaagttga
     3241 tcgacaccgc cgcggggatc gccgcgagcc ggcatgccgg ggggccggcg gtgacggtgt
     3301 cgcttgacca gatgagcttt ctctcgccgg tgcgcgtcgg gaacgtggtg ttcgtgcatg
     3361 ccagcgtcaa cgacgtcggg cggacctcga tggaagtcgg cgtccgggtc gaggctgagg
     3421 acttcgtgac cgggcagcgg acccatacct actcggcgta tctggtgttc gtcgcgctgg
     3481 acgagcagca gcgaccccgg ccggtgccgc cgctggagcc ggagaccgag gtggagcggc
     3541 ggcggcaggc cgaggcgaag atccgccggg agttccgcct ggccc
//
LOCUS       DASWDC010000101         7604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1359905, whole genome shotgun sequence.
ACCESSION   DASWDC010000101 DASWDC010000000
VERSION     DASWDC010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359905"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VF995_03275"
     CDS             complement(<1..387)
                     /locus_tag="VF995_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX9342623.1"
                     /translation="MEAIHPKVEEAVDTLELNPVTRRRLLTGVGLTSASAAAAALLAA
                     CSSKKSTTSAKASGAGQFPSTPKWKFTFINHVTTNPFFVPTQYGLADAASLLGIPKPA
                     WTGSANSSVPEMVSATNAAISSKANGI"
     gene            complement(523..1665)
                     /locus_tag="VF995_03280"
     CDS             complement(523..1665)
                     /locus_tag="VF995_03280"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015621662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX9342624.1"
                     /translation="MAVIIAAFGMAASAASPAHPDPSITKAPFGTTPDGQAVARYTLS
                     NSHGMQVRIITYGGIVQSIKVPDRHGHFTDVALGFATLDDYIAHNNPGPYFGALIGRY
                     ANRIAKGTFTLDGKTYHLPINNPPNTLHGGFKGFDTKVWTVDGTETGKDSVSLELSLV
                     SPNGDQGYPGRLTAHVTYTVTNRDEIRIDYRATTNAPTIVNLTNHSYFNLSGEGTGDI
                     YGEKMLINASHYTPVDSTLIPTGQIAPLAGTPLDFSTPTAIGARIRDNFQQLVFGRGY
                     DHNFVLNRPAGDTTTLELAARVTDPASGRVLELFTDQPGMQFYSGNFLDGTLYGTSGH
                     QYRQSDGFALETQHFPDSPNHPNFPSTVLRPGQTYRTTTVYRFSTD"
     gene            complement(1795..2361)
                     /locus_tag="VF995_03285"
     CDS             complement(1795..2361)
                     /locus_tag="VF995_03285"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX9342625.1"
                     /translation="MNPPSSWNPLDPMVLSARASGPDADAGAAVGLKLLEARRLVEVG
                     VAELAAVRRSEAQLDAMTQALVQQRGAHDAGDTDRFVAADIRFHQALMEAAGNEVVAA
                     LFGPIQSLVYEGRRQTSSWPDARALAIRAHERILTAVRRQDPGAARRRMLDHLTATAA
                     ALEEHVATPDSRSEPSTPEAEVAIDRST"
     gene            complement(2659..3822)
                     /gene="xylA"
                     /locus_tag="VF995_03290"
     CDS             complement(2659..3822)
                     /gene="xylA"
                     /locus_tag="VF995_03290"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003977662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEX9342626.1"
                     /translation="MAYEPTRADRFSFGLWTVGHPGHDPFGDPVRPWLDPVETVQRLS
                     DLGAYGVTFHDDDLLPPGSDGSVAERAKALKRFRAALDQTRMTVPMATTNLFRHPVFR
                     EGAFTANDPAVRRHAIRKVLANLDVAAELGARVYVCWGGREGCESDTKDVRAALDRYK
                     EAIDLLCAYVRERGYQLRFALEPKPNEPRGDIFLATVGHALAFIERLEHPELVGLNPE
                     VGHETMAGLNFHHGVAQALWAGKLFHIDLNAQRGPRYDQDLRFGSEDQKGAFFLVDLL
                     ERAGWAGPRHFDARAYRTEDPEGVWQFARGCMRTYLILRERAAAFRADPEVAEAMAAA
                     GIPDLATPTLRPGETLEGLRAADASFDPEARARRGSGYERLDQLVVDHLLGAR"
     gene            complement(3841..4953)
                     /locus_tag="VF995_03295"
     CDS             complement(3841..4953)
                     /locus_tag="VF995_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018893266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9342627.1"
                     /translation="MVGYAFMGRAHSQGWRTAGHFFDLPLTPRMAVICGRDQGKVARA
                     AERLGWAAWETDWKRLVARDDVQLVDVCTPGSSHAEISIAALEAGKHVLCEKPLANSV
                     AEARAMAEAAERAARRGVRAMVGFNFRRLPAVAFARELIAAGRLGTIRQARGAYLAAH
                     AADPGTPLAWRHQVEAAGSGALGDLGAHVVDLTQYLIGDRIAGVAALTETFTRERPLT
                     AEGGPHGPVTVDDAALFIARFTGGATGSFEATRVATGHNEDLRVEVNGEHGSLVFDLG
                     RLNELHYYAAGEPATSGFRRIQVTRAEHPYAGAWWPDGHTIGYEHTFTHELRDLLEAI
                     AAGNDPVPSFADGLQVQQVLDAVLRSAAAGSVWTAV"
     gene            5144..6565
                     /gene="xylB"
                     /locus_tag="VF995_03300"
                     /pseudo
     CDS             5144..6565
                     /gene="xylB"
                     /locus_tag="VF995_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574509.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
     gene            6618..>7604
                     /locus_tag="VF995_03305"
     CDS             6618..>7604
                     /locus_tag="VF995_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX9342628.1"
                     /translation="MTPQPAPARQRSLREHNLALVLQRLASDGPASRAQLAAVTGLTK
                     ATVSSLVDDLVAGRLVVELGPEARGAVGRPGSALALNRTGLAGVGLEVNVDYLAVCVT
                     DLAGEVRYRRTRAGDNRRQSPGRVLARAARMARAALAAAEAEGLAAAGLAAALPGLVE
                     TEQGLLRTAPNLGWQDVPVGEFFSGRFRLPVAVDNEANLAALGELWFGGHDGPNGILT
                     DFVHVSGEIGVGGGIVIGRELFRGVRGFAGEIGHLTVQPDGPPCRCGGRGCLEQLAGQ
                     EAILRAAGLSGAAGTSMGDPGGSVAALVARARAGDSRTLQAIEAAAHALGIAI"
BASE COUNT          934 a         2740 c         2821 g         1109 t
ORIGIN      
        1 gatcccgttc gccttcgacg agatggcggc gttggtggcg ctgaccatct cggggacgct
       61 cgagttcgcc gagccggtcc aggcgggctt ggggatcccc agcagcgacg ccgcatcggc
      121 cagcccgtac tgggtgggga cgaagaacgg gttggtggtg acgtggttga tgaacgtgaa
      181 cttccacttg ggcgtggacg ggaactgccc ggcgccggac gccttggcgc tggtggtgct
      241 cttcttcgac gagcaggcgg cgagcagcgc cgccgccgcg gccgaggcgc tggtcaaccc
      301 gacgccggtc agcagccgcc gccgggtcac cggattgagt tcgagcgtgt cgacggcttc
      361 ttcgaccttg ggatggatcg cttccatcgc agacctccca ccgctggagt ctgaagttga
      421 gtttcccgaa ctctgcgagc cttgccaagg gccttgctgt gaaggccgcc gtcccggccc
      481 tcggggggac ggcggccgtc gtgctgagtc gtgctgatca ggtcagtcag tcgagaaccg
      541 gtacaccgtg gtggtgcggt aggtctggcc cggccgcagc accgtcgaag ggaagttggg
      601 gtggttcggc gagtcgggga agtgctgggt ctccagcgcg aacccgtcgc tctgccggta
      661 ttggtggccg ctggtgccgt acagggtccc gtccaggaag ttcccggagt agaactgcat
      721 cccgggctgg tcggtgaaca gctccagcac ccgcccgctg gccgggtcgg tcacccgggc
      781 cgccagctcc agcgtggtgg tgtcgccggc ggggcggttc agcacgaagt tgtggtcgta
      841 gccgcgcccg aacacgagct gctggaagtt gtcgcggatg cgcgccccga tcgccgtcgg
      901 cgtggagaag tccagcggcg tcccggccag cggcgcgatc tggccggtgg ggatcagggt
      961 ggagtcgacc ggcgtgtagt ggctggcgtt gatcaacatc ttctcgccgt agatgtcgcc
     1021 ggtgccctcg ccggagaggt tgaagtagga gtggttggtg aggttgacga tcgtcggcgc
     1081 gttggtggtg gcgcggtagt cgatacggat ctcgtcgcgg ttggtgaccg tgtaggtcac
     1141 gtgcgcggtg agcctgcccg ggtagccctg gtcgccgttc gggctgacca ggctcagctc
     1201 caggctgacc gagtccttcc cggtctcggt cccgtccacc gtccagacct tggtgtcgaa
     1261 gcccttgaag ccgccgtgca gcgtgttggg cgggttgttg atcggcaggt ggtaggtctt
     1321 gccgtcgagg gtgaaggtcc ccttggcgat acggttggcg taccggccga tcagcgcgcc
     1381 gaagtacggg cccgggttgt tgtgggcgat gtagtcgtcc agggtggcga agccgagcgc
     1441 cacgtcggtg aagtggccgt ggcggtcggg gaccttgatc gactggacga tgccgccgta
     1501 ggtgatgatc cgcacctgca tgccgtggct gttgctgagc gtgtagcggg ccacagcctg
     1561 gccgtccggg gtggtgccga acggcgcctt ggtgatgctc gggtcggggt gcgcgggcga
     1621 cgccgccgac gcggccatcc cgaaggccgc gatgatcacg gccatgagga ccgctgcggt
     1681 gccacgccgc cttgcggtgg cggtttccca tccggtgcgg agtcggtaca tggaccctcc
     1741 ttggtcgtcc atccggacat ccgaaacggg ctgcggagct gggctgtgga ccggtcaggt
     1801 ggagcggtcg atcgctacct ctgcctctgg ggtcgatggt tcggatcggg aatcgggagt
     1861 ggcgacgtgc tcttcgagcg cggccgcggt cgccgtcagg tggtcgagca tccgccgccg
     1921 cgcggcgccc gggtcctggc ggcggaccgc cgtcaggatc cgctcgtggg ccctgatcgc
     1981 cagcgcccgc gcgtcgggcc agctggaggt ctggcgccgg ccctcgtaga cgaggctctg
     2041 gatgggtccg aacagggcgg ccaccacctc gttgccggcg gcctccatca gcgcctggtg
     2101 gaacctgatg tcggcggcca cgaagcggtc ggtgtcgccc gcgtcgtgag cgccgcgctg
     2161 ctggaccagc gcctgggtca tggcgtcgag ctgtgcctcg ctgcgccgca ccgccgccag
     2221 ctcggccacg ccgacctcga ccagccgccg ggcctccagc agcttcagcc cgaccgccgc
     2281 gcccgcgtcc gcgtccgggc cggacgcgcg ggcgctgagg accatcgggt cgagcgggtt
     2341 ccagctggac ggcgggttca cgaaggtgcc tgccctgctc gacgcggacc acgcccatgg
     2401 cctggagcac cttcatcgcc tcccgcagcg tcaggcggct gacgccggtc atcgcgacca
     2461 gctccggttc cggcggcagt cgcgaccccg gctcgtaggc gcccccgacg atcgcggcga
     2521 ggaactcgtg ggcgacctgg gcggggagcg actgccgccg gatccggaac ggcagctgag
     2581 gcttgatcat cagatgttca gagtggaacg ggtggcagac catgtcaaga gccgcgttcg
     2641 cgctgccccc ccggctcctc atcgggcgcc cagcaggtgg tcgacgacca gctggtcgag
     2701 ccgctcgtag ccgctccccc gccgggcccg ggcctccggg tcgaacgagg cgtcggcggc
     2761 gcgcagcccc tccagcgtct cccccggccg cagcgtcggc gtggcgaggt cggggatccc
     2821 ggcggcggcc atcgcctcgg cgacctccgg gtcggcgcgg aaggcggcgg cgcgctctct
     2881 caggatcaga tacgtccgca tgcagccacg cgcgaactgc cagacgccct cggggtcctc
     2941 ggtgcggtag gcgcgcgcgt cgaagtgccg gggcccggcc cagcccgcgc gctccaaaag
     3001 atccaccagg aagaaggcgc ctttctggtc ctccgacccg aagcgcaggt cctggtcgta
     3061 acggggcccc cgctgcgcgt tcaggtcgat gtggaacagc ttgccggccc acagcgcctg
     3121 tgccacgccg tggtggaagt tcagcccggc catggtctcg tgcccgacct cggggttgag
     3181 gccgaccagc tccgggtgct ccagccgctc gatgaacgcc agcgcgtgcc cgacggtggc
     3241 caggaagatg tccccgcgcg gctcgttggg tttcggctcc agcgcgaacc tgagctgata
     3301 cccgcgctcc cggacatagg cgcagagcag gtcgatggct tccttgtagc ggtcgagggc
     3361 ggcgcggacg tccttggtgt cggactcgca gccctcccgg ccaccccagc agacgtagac
     3421 ccgcgcgccc agctcggccg ccacgtcaag gttggccagg acctttctta tcgcatggcg
     3481 cctgaccgcc gggtcgttgg cggtgaaggc cccctcccgg aacaccgggt ggcgaaacag
     3541 gttcgtcgtg gccatcggca cggtcatcct ggtctggtcg agcgccgcgc ggaatcgctt
     3601 gagcgccttg gcccgctcgg cgacgctccc gtccgatccc ggcggcagca ggtcgtcgtc
     3661 gtggaaggtc accccgtagg cgcccaggtc cgagagtcgc tggaccgtct cgaccgggtc
     3721 cagccagggc cgcaccgggt ccccgaacgg gtcgtgcccg ggatgcccga ccgtccacag
     3781 cccgaagctg aacctgtccg cgcgggtcgg ttcgtatgcc atctccgatg ccatctccga
     3841 tcagaccgcc gtccagacgg aaccggccgc cgcgctgcgc aggaccgcgt cgaggacctg
     3901 ctggacctgg agcccgtcgg cgaagctcgg gaccgggtcg ttgccggcgg cgatcgcctc
     3961 cagcagatcg cgcagctcat gggtgaaggt gtgctcgtag ccgatggtgt ggccgtcggg
     4021 ccaccacgcc ccggcgtagg ggtgctcggc cctggtcacc tggatgcggc ggaagccgct
     4081 ggtggccggc tccccggcgg cgtagtagtg cagctcgttg aggcggccga ggtcgaagac
     4141 gaggctgccg tgctcgccgt tgacctcgac ccgcaggtcc tcgttgtgcc cggtggcgac
     4201 gcgggtcgcc tcgaagctgc cggtcgcgcc gccggtgaac cgggcgatga acagcgcggc
     4261 gtcgtcgacg gtgaccggcc cgtgcggccc gccctccgcg gtgagaggcc gctccctcgt
     4321 aaaggtctcg gtcagcgcgg cgacgccagc gatacgatcg ccgatcagat attgcgtgag
     4381 gtcgacgacg tgggcgccca ggtcgccgag cgcgcccgac cccgccgcct cgacctggtg
     4441 gcgccaggcc agcggcgtcc cggggtcggc ggcgtgggcg gccaggtagg cgccgcgcgc
     4501 ctggcggatc gtgccgaggc ggccggcggc gatcagctcg cgggcgaacg cgaccgccgg
     4561 caggcggcgg aagttgaagc cgaccatcgc ccgcaccccg cgccgggcgg cccgctcggc
     4621 ggcctccgcc atggcccgcg cctcggccac gctgttggcc agcggcttct cgcagagcac
     4681 gtgcttgccg gcctcaagcg ccgcgatcga gatctcggcg tggctcgacc cgggcgtgca
     4741 gacgtcgacc agctgcacgt cgtcgcgggc gaccagccgc ttccagtcgg tctcccaggc
     4801 ggcccagccg agccgttcgg cggcccgcgc gaccttgccc tggtcgcggc cgcagatcac
     4861 cgccatgcgc ggggtgagcg gcaggtcgaa gaagtggccg gcggtgcgcc acccctgcga
     4921 gtgggccctg cccatgaagg cgtagcccac catccccacg ccgagcgaac tggtgctcac
     4981 ggcagcccct gggtcggcct tccagatgat ttgtcagttc accatacgaa ctaaagcgcc
     5041 cgcgccgaac catgtcaatg gcgtagttgt gatcttcagc aaggcgacgt tgcatagtat
     5101 gttgagtgac tgaacgaaag atcccccggg gaggagcctt gccatggccg ctgtcgccgg
     5161 catcgactgc tcaacccaag ccaccaaggt cctggtctgc gacgccgaca gcggcgagcc
     5221 ggtcgccgag cgctccgcgc agcaccccga cggcaccgag gtcgacccgg cggcctggtg
     5281 gggcgcgttc gaggcggcct cggccgggct gctcgacggc gtgcgcgcgg tcgccgtcgg
     5341 cggccagcag cacggcctgg tcgccctcga ccagaccggc gcggtggtgc gcccggcgct
     5401 gctgtggaac gacgtccgct ccgccggcgc cgccgaagat ctgatcaagg aactgggagg
     5461 gccggccgcc tgggcggctg ctgtggggct ggtgccggtc gccagcttca ccatcaccaa
     5521 gctgcgctgg ctagcgaccc acgagcccgg ccacgccgcc cgggtcgcct ccgtgttgct
     5581 cccccacgac tggctcacct ggcgcctggc gggatcgggc acgcccgtca ccgaccgggg
     5641 cgacgcctcc ggcaccggct actgatcccc gcgcaccggc gcctaccgta ctgacctgct
     5701 ggaattggcg ttcggccggg tgccctccct gccgcaggtg ctggcacccg acgaggcggc
     5761 cggcgagacc gccagcggcg cggtggtcgg cgccgggacc ggggacaaca tggccgccgc
     5821 gctcggtctg ggcgccgggc cgggcgacgt ggtggtgtcg gtcggcacca gcggcacggt
     5881 gttcgccgtc cacgaggagc ccgccgagga cccgagcggc accgtcgccg ggttcgccga
     5941 cgcgaccggg cggttcctgc cgctggtctg caccctgaat gccgcacggc cgctcggcgg
     6001 ggtcgcggcc atgctcggcg tcgacctggc cgccctcgac cgtctcgcgc tggccgcccc
     6061 gccgggcggc ggcgggctgg tgctgctccc ctacctcgac ggcgagcgca ccccgaacct
     6121 ccccagcgcc agcggcctgc tcgccgggct gcgcctggcc aacatgacgc ctgagaacct
     6181 cgcccgcgcc gccgtcgagg gcgtgctctg cggcctcgcc gacggcctcg acgccctccg
     6241 cgccgtcggc gtgccggtcc ggcgggtgct gctgatcggc ggcggcagcc gatcggcggc
     6301 ggtccgcgcg ctcgcccccg cgatcctcga cgccccggtg gaggtgcccc gccccgccga
     6361 gtacgtcgcc cggggcgccg cccgccaggc cgcctgggcg ctcagcggcg cccccgaacc
     6421 gccgccctgg ccgctcgccc gcacggccgg cgcggatgtc caggtggagg gcgggccgcc
     6481 tggcgtgggt gcccggatcc gtgcccgcta cgccgaggcg cgcgagggcg ccagcgccgg
     6541 gctgtggggc ggagccatcc ggtgaggcaa gcgcgcgtgc tccatgtgcg acgaccccgg
     6601 agaggagctg tgacgcggtg acgccgcagc cggcgcccgc gcggcagcgc tccctgcggg
     6661 agcacaacct cgccctggtg ctgcagcggc tggccagcga cgggccggcc tcccgcgccc
     6721 agctcgccgc cgtcaccggg ctgaccaagg cgaccgtctc cagcctggtc gacgatctgg
     6781 tggcgggccg gctggtggtc gagctgggcc ccgaggcgcg cggcgcggtc ggcaggcccg
     6841 gcagcgcgct cgccctcaac cggaccggcc tcgccggcgt gggcctggag gtgaacgtcg
     6901 actacctggc ggtctgcgtc accgacctgg ccggggaggt ccgctaccgg cgcacccggg
     6961 cgggcgacaa ccgccgccag tcgcccggcc gggtgctcgc ccgcgccgcc cgcatggccc
     7021 gcgccgcgct ggccgccgcc gaggcggagg ggctggccgc cgccggcctc gccgccgccc
     7081 tgcccggcct ggtcgagacc gagcagggac tgctgcgcac cgcccccaac ctcggctggc
     7141 aggacgtgcc ggtgggtgag ttcttcagcg gccgcttccg cctgcccgtc gccgtggaca
     7201 acgaggccaa cctggccgcc ctcggcgagc tgtggttcgg cggccacgac ggcccgaatg
     7261 gcatccttac cgacttcgtg cacgtctccg gcgagatcgg ggtcggcggc ggcatcgtca
     7321 tcggccgcga gctgttccgc ggggtccgcg gcttcgcggg cgagatcggc cacctcaccg
     7381 tccagcccga cggacccccc tgccgctgcg gcggccgcgg ctgcctggag cagctcgccg
     7441 gccaggaggc gatcctgcgc gccgccggcc tcagcggcgc ggccggcacc agcatgggcg
     7501 atcccggcgg ctcggtcgcc gccctggtcg cccgcgcccg cgccggcgac tcccgcaccc
     7561 tccaggcgat cgaggccgcc gcccacgcgc tcggcatcgc catc
//
LOCUS       DASWDC010000102         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1611058, whole genome shotgun sequence.
ACCESSION   DASWDC010000102 DASWDC010000000
VERSION     DASWDC010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611058"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VF995_03310"
     CDS             complement(<1..286)
                     /locus_tag="VF995_03310"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX9342629.1"
                     /translation="MGPWPPSATSARSAVAAALAVAEHADASGEDLLRAVTIGYEVGA
                     RVALAVGPAHYQHWHTTGTVGALGAAAAAAEVLRLDAGQFANALATAATMS"
     gene            174..1091
                     /locus_tag="VF995_03315"
     CDS             174..1091
                     /locus_tag="VF995_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9342630.1"
                     /translation="MVTARSRSSPEASACSATASAAATAERAEVADGGHGPIVLPAGR
                     SWWQRHGETTTVWTLRLLLLGAVLAAWQLTAGHLFDRLFTSSPSEVAAKLDTWARDGT
                     LWSNTWVTIQEIVVGYVFGAAAGVLAGFALASQRIAWRVLEPVVMALYAIPKVALAPL
                     FIIWFGIGLKMKVLVAAVSVFFLVFINTAAGVHAVDQSLVDAVRLMGGKRRDITLKVM
                     LPGAMSGVLTGLRVAIPYALIGAVIAELVASNRGLGYLISDSAARFDTAGVFAALVVL
                     SVIAAVLGALVNVLGRYANRWKPLEQRHH"
     gene            1164..2261
                     /locus_tag="VF995_03320"
     CDS             1164..2261
                     /locus_tag="VF995_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9342631.1"
                     /translation="MRTPDPGMGKGTRTGRGHGLGWRARLLAAGAVLVLVAAGCKSSG
                     GGSGGGGGSDMTLNVGRVSNSVAFLPFYVAERQGYFAAEGVKLGDQPVLGTGAKVAAA
                     LSSGSIDIGGSVMTDVLNMARAHQDVKLLAPLVNAYYIDVTVSSRFAQPSGDLNAKIM
                     ALKGRKIGITGPGSGTEALLVYLFKQVGLDTKKDATLMPLGSDATAALGALKSGRVDA
                     LSLAQPVGELVEAQGIGTIYISPSRGDIPALRGATQGVAFTTQSAINRKGKAIAAFLR
                     AIAKAEQLIHSDAATTQKLLAGYQPTMKPATVEKLVPILRQEIPATPTFTQQGYQTIV
                     TFHTSSGLVSSAPPFASVVPTAWINQALAGG"
BASE COUNT          337 a          888 c          892 g          331 t
ORIGIN      
        1 cgctcatggt ggcggcggtg gccagggcgt tggcgaactg gccggcgtcc agcctgagca
       61 cctcggcggc ggccgcggcg gcgccgaggg cgccgactgt cccggtggtg tgccagtgct
      121 ggtagtgcgc ggggccgacc gccagcgcga cgcgcgcgcc gacctcgtag ccgatggtca
      181 cggcgcggag caggtcctcc ccggaggcgt cggcgtgctc ggcgaccgcc agcgccgccg
      241 cgaccgccga gcgcgccgag gtggccgacg gcggccatgg cccgatcgtg ctgccggccg
      301 gtcgcagctg gtggcagcgg cacggcgaga ccaccacggt gtggacgctg cggctgctgc
      361 tgctcggggc cgtgctggct gcctggcagc tcaccgccgg ccacctgttc gaccggctgt
      421 tcaccagcag cccctcggag gtggccgcca agctcgacac ctgggcgcgc gacggcaccc
      481 tgtggtccaa cacctgggtc accatccagg agatcgtcgt cgggtacgtg ttcggcgcgg
      541 cagccggggt gctcgccggg ttcgcgctgg cctcccagcg gatcgcctgg cgggtgctgg
      601 agccggtcgt gatggcgctg tacgccatcc ccaaggtggc gctggcgccg ctgttcatca
      661 tctggttcgg catcggcctg aagatgaagg tgctggtcgc cgcggtcagc gtcttcttcc
      721 tggtgttcat caacaccgcc gccggtgtcc acgcggtgga ccagagcctg gtggacgcgg
      781 tgcggctgat gggcggcaag cggcgcgaca tcaccctcaa ggtgatgctg cccggggcca
      841 tgtccggcgt gctcaccggc ctgcgggtgg cgatccccta cgccctgatc ggcgccgtca
      901 tcgccgagct ggtcgcctcc aaccgcggcc ttggctacct gatcagcgac tccgcggcca
      961 ggttcgacac cgccggggtg ttcgccgcgc tggtggtcct gagcgtgatc gcggcggtac
     1021 tcggcgccct cgtcaacgtc ctgggccggt acgccaaccg ctggaagccg ctcgagcagc
     1081 gccaccactg accccccgcc gcctcccccc aaagcgcacg acagcgtctc cccagcacag
     1141 cccctccgat ggaaggagca gcgatgcgaa ccccggatcc gggtatgggc aagggcacca
     1201 ggaccggtcg cggccacggc cttggctgga gggcccggct gctggccgcg ggcgccgtcc
     1261 tcgtcctggt cgccgccggc tgcaagtcca gcggcggcgg cagcggcggg ggcggcggca
     1321 gcgacatgac cctgaacgtc gggcgggtca gcaactcagt cgcgttcctg cccttctacg
     1381 tcgccgagcg tcagggctac ttcgccgccg aaggggtgaa gctcggcgac cagccggtgc
     1441 ttgggaccgg cgccaaggtc gccgccgcgc tgtcctccgg gtcgatcgac atcggcggga
     1501 gcgtcatgac cgacgtgctg aacatggcca gggcccacca ggacgtgaag ctgctcgcgc
     1561 cgctggtcaa cgcctactac atcgacgtca ccgtgagcag ccgcttcgcc cagccgagcg
     1621 gcgacctgaa cgccaagatc atggcgctga agggcaggaa gatcggcatc accggcccgg
     1681 gcagcggcac cgaggcactg ctggtctacc tgttcaagca ggtcgggctg gatacgaaga
     1741 aggatgccac cctgatgccg ctcggcagcg acgccaccgc ggcgctcggc gcgctcaaga
     1801 gcgggcgggt ggacgcgctc tcgctcgccc agccggtcgg cgagttggtg gaggcgcagg
     1861 gcatcggcac gatctacatc tcgcccagcc ggggcgacat ccccgcgctg cggggcgcga
     1921 cccagggggt ggccttcacc acccagtcgg cgatcaaccg caagggcaag gcgatcgccg
     1981 cgttcctgcg tgcgatcgcc aaggcggagc agctgatcca ctccgacgcc gccaccaccc
     2041 agaagctgct cgccggctac cagccgacca tgaagcccgc caccgtcgag aagctggtcc
     2101 cgatcctgcg gcaggagatc cccgccacgc cgaccttcac ccagcagggc taccagacca
     2161 tcgtcacgtt ccacaccagc agcggcctgg tcagctccgc gcccccgttc gcgtcggtcg
     2221 tcccgacggc ctggatcaac caggccctcg cgggcgggta gggatcggga agggatcggg
     2281 accgcgtcgc gccgcgcggg caggggcacg ggcgcgggca ggggcgcggg cgtgggcgcc
     2341 ggtgtcaggt cgccggcggc cccgccaggg tgcggaggac cgccgccggg ttggagagca
     2401 cccgccggat gaccgtgccc gccgcgcccc tgaccgcggc gtcggggc
//
LOCUS       DASWDC010000103         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1611200, whole genome shotgun sequence.
ACCESSION   DASWDC010000103 DASWDC010000000
VERSION     DASWDC010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611200"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VF995_03325"
     CDS             complement(<1..274)
                     /locus_tag="VF995_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011208811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9342632.1"
                     /translation="MDETEAGTAREHLAANRALWDEWTGVHLRSEFYDVAGWKAGRRP
                     MRAFLLEELGDVNGKDVLHLQCHFGLDTLALARLGARVTGVDFSERA"
     gene            complement(361..1380)
                     /locus_tag="VF995_03330"
     CDS             complement(361..1380)
                     /locus_tag="VF995_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9342633.1"
                     /translation="MEFPVALQRSRAESQGQQPLAAYRWISSLPLLAVTFGAVVIAFS
                     AILVRLSGASPEAAATYRCVYAVPVLWLLAFLEDRQFGRRPLGDRLLAVPAGVLLAAD
                     LILWHHAIGAVGAGLATVLANLQVVIVGLAAWVLLHERPGRLLAAVPLALFGVLLVSG
                     LLERGASGSDPALGVLFGSVASLCYAAFLLLLRHLGRDLRRPAGPLFDVTAVAAVGCA
                     AAGLVLDPRSLVPSWPAQGWLVLLALLPQVVGWLLISVALPRLPAALGSALLLIQPAS
                     SVGPRSGVARRASLGLPVARRCPDSRRGAACRSGRGVAVTSATQRGRGSHSPWPNVGP
                     PMRRT"
     gene            1555..2886
                     /locus_tag="VF995_03335"
     CDS             1555..2886
                     /locus_tag="VF995_03335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9342634.1"
                     /translation="MTSTGASQPQPGDGTPPPVKLHGWRDAAILTVAGLTVAAGFAQF
                     GATAALGDVAKTFGAAATGRSIAAQVGLSGTTLGLGLAIIRLASLLSLPLTGLADRHG
                     RRRTLISCVTIGLALTATAALSPTYWWFVALFAAGRPLLSATNALSGVVAAEETQTRD
                     RAKAIAMVTAGYGLGAGLTALARGTAGNHLSFRGLFALALIPLAAMPLLTRRLEEPDR
                     YRRLAHTDPATAAATLPPGGLRQLWAPGRVRAELRSRLWLLTALYFSFSFVTGPAVTF
                     VFLYAENALGMPRSATTLMVLAAGPLGLLGLLSGRWAADTLGRRRAAIGAQASVALAG
                     IITYSGTPAGAIAGYLLGIIAGGAYSPASWALAAELFPTSLRATIFGWLTAANVLGAV
                     FGLIIFGRLADSLSSFPLAATIICIPVIAASLFYIRLPETAGLELEQSAPE"
BASE COUNT          532 a         1257 c          818 g          336 t
ORIGIN      
        1 ccgcccgctc agagaaatcc accccggtca cccgcgcccc caaccgcgcc aacgcgagcg
       61 tgtcaagccc gaaatggcac tgcaggtgca gcacgtcctt gccgttcacg tcgccaagct
      121 cctccagcag gaacgcccgc atcggcctgc gtcccgcctt ccaccccgcc acgtcataga
      181 actccgagcg gagatgcacc ccggtccact cgtcccacag cgcccggttc gccgcgagat
      241 gctcccgcgc cgtccccgcc tccgtctcgt ccatggtcca agcctcccac ccgcccgggg
      301 gtaaccgcag ccggttttcc acaatttcgg ccgcaccctc cacccggatc attgcttcgg
      361 ttacgttcgg cgcatcgggg ggcccacgtt cggccacgga gagtgagagc cacgaccacg
      421 ttgagtcgcc gaggttacag cgaccccccg gcccgagcgg caagcagcac cccgacgaga
      481 atcagggcaa cgccgagcaa ctggaagtcc gagggaagct cgccgagcaa cacccgagcg
      541 aggacccacc gaggacgccg gctggataag cagcaacgcc gagccgagcg cggcgggcag
      601 acgcgggagc gcgaccgaga tcagcaacca cccgacaacc tgcgggagca gcgccagcag
      661 cacaagccag ccctgcgccg gccacgacgg caccaggcta cgcggatcca ggacaaggcc
      721 ggcggcagca caacccaccg cggcgacggc ggtcacgtcg aacagggggc ccgctgggcg
      781 gcggagatcc ctgcccagat gacgcaacaa cagcaggaac gccgcatagc agagcgaagc
      841 aactgagccg aacagcacac ccaacgcagg atcgctccca gaggcgcccc gctccagcag
      901 acccgagacc agcagcaccc cgaacagcgc cagcgggacc gcagccagca gccgaccggg
      961 ccgctcatgc aacagcaccc acgcggcaag cccgacgatc accacctgca ggttggcgag
     1021 caccgtcgcc aaacccgcgc cgaccgcccc gatcgcgtga tgccacagaa tcaggtcagc
     1081 ggcgagcagg acaccggctg gcaccgccag cagccgatcc ccgagcggac gccgcccgaa
     1141 ctggcgatcc tcaaggaagg cgagcaacca caggaccggc accgcgtaga cacaccggta
     1201 ggtagcagcc gcctccgggg acgcgccgga aagacgcacc agaatcgccg aaaacgcgat
     1261 caccaccgcg ccgaacgtga ccgccagcag cggcaacgac gagatccacc gataggcggc
     1321 gagcggctgc tgaccctgtg actctgcccg cgaccgctgc agagccacag ggaattccac
     1381 ctgatgccac tcctgccctc ggacaacgcg tcccctcaca gtgccagaaa gccaggcagg
     1441 cagcccaacg acaacgatcg gcgaaccgca ccggtaccac ccccggaccg ggcgtaaagt
     1501 gcctgccagc gcaccgcgcc gccgcgcccc gcccaacgaa cgggaacaac cgcattgacc
     1561 tcgaccggcg ccagccagcc acaacccggg gacggcacac cgccgccggt caagctccac
     1621 ggctggagag acgcggcgat cctcaccgtc gccgggctca ccgtcgcagc cgggttcgca
     1681 cagttcggcg caaccgccgc actcggcgac gtcgccaaaa ccttcggcgc cgcagcgacc
     1741 ggccggtcaa tcgccgcaca ggtcggactc tccggcacca ccctcggact cggcctcgcg
     1801 atcatccggc tcgcctcact cctcagcctc ccactcaccg gcctcgccga ccgccacgga
     1861 cggcgccgca ccctgatcag ctgcgtcacc atcgggctcg ccctcaccgc caccgccgcg
     1921 ctcagcccca cctactggtg gttcgtcgcc ctcttcgccg ccggacggcc gctcctctcc
     1981 gccaccaacg ccctctccgg cgtcgtcgcc gccgaagaaa cccaaacccg cgaccgagcc
     2041 aaagccatcg ccatggtcac cgccggatac ggcctcggcg ccggcctcac cgccctcgcg
     2101 cgcggcaccg caggcaacca cctcagcttc cggggactgt tcgcactcgc cctcatcccc
     2161 ctcgccgcga tgccactcct cacccgacgc ctcgaagaac ccgaccgcta ccggcgcctc
     2221 gcccacaccg acccagcaac cgccgccgcc acgctccccc cgggcggcct ccggcaactc
     2281 tgggcacccg gccgcgtccg cgccgaactc cggtccaggc tgtggctgct caccgccctc
     2341 tacttctcgt tctcgttcgt caccgggcca gcggtcacct tcgtgttcct ctacgccgaa
     2401 aacgccctcg gcatgcccag atccgctacc accctcatgg tcctcgccgc cggccccctc
     2461 ggcctcctcg gcctcctcag cggccgctgg gccgccgaca ccctcggccg ccgccgcgcc
     2521 gccatcggcg cccaagccag cgtcgccctc gccggcatca tcacctacag cggcaccccg
     2581 gcgggcgcca tcgccggcta cctcctcggc atcatcgccg gaggcgccta ctcccccgcc
     2641 agctgggccc tcgccgccga actcttcccc accagcctcc gcgccaccat cttcggctgg
     2701 ctcaccgccg ccaacgtcct cggcgcggtc ttcggcctga tcatcttcgg ccgcctcgcc
     2761 gacagcctct ccagttttcc tctcgccgcc acgatcatct gcatccccgt gatcgccgcc
     2821 agcctcttct acatccgcct ccccgaaacc gcaggattgg aacttgagca gtccgccccg
     2881 gagtaacgct cacgcggtga gcaccgtccg gagttccgcc acgccgtagg tcgtgctggc
     2941 cga
//
LOCUS       DASWDC010000104         7839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_97362, whole genome shotgun sequence.
ACCESSION   DASWDC010000104 DASWDC010000000
VERSION     DASWDC010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97362"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VF995_03340"
     CDS             <1..581
                     /locus_tag="VF995_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX9342635.1"
                     /translation="RTAHVIVNGELVDGLGGGVCQAGTTMFNAALFGGLPVLERRNHS
                     LHISHYPLGRDATLNWPDTDLKFKNDSPYGIYITSTWSASSLTFTLWSSSRHYQVSLA
                     TSAESNFRDPPTKVEDDPSLPAGQQQPKETGSAGFDVTVTRTVTLSGQLVRRDQFTSR
                     YDPWPTIISRGTGQPAPATTVPATAIATGTSA"
     gene            623..1003
                     /gene="gcvH"
                     /locus_tag="VF995_03345"
     CDS             623..1003
                     /gene="gcvH"
                     /locus_tag="VF995_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX9342636.1"
                     /translation="MYPEDLKYTREHEWARIKRGRATMGITDYAQDALGDIVYVDLPA
                     VGATVTNGEALGEVESTKSVSELFSPVSGTVTERNNELDKHPEIINADPYGQGWLLVI
                     ELSDPAEAGQLLDAADYSRLVEES"
     gene            1180..1665
                     /locus_tag="VF995_03350"
     CDS             1180..1665
                     /locus_tag="VF995_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX9342637.1"
                     /translation="MFCTQCGHRNPDGAHFCANCGTALVPPPDDSTTTISLAPQDLEA
                     EVEDEVAAPVEDLREGTAMLVVRRGPNAGSRFLLDKEVVTAGRHPESDIFLDDITVSR
                     RHAEIRRTPGGFTVHDVGSLNGTYLNRERVEDGELVGGDELQIGKFKLVFFAGGRPGG
                     G"
     gene            1746..2741
                     /locus_tag="VF995_03355"
     CDS             1746..2741
                     /locus_tag="VF995_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9342638.1"
                     /translation="MPSRPHPRRDQATGPAGTMSIGEVLAILKPEFPDITVSKIRFLE
                     GAELVRPGRSASGYRMFSEDDVARLRFVLRAQRDQYLPLRVIRQRLAELDQVGGLYAR
                     GGTLAHGPEPGGEASPTGPTGSGSGSGSAAAGRAGPAAGGRRTRTRSATGAGLPPGGA
                     AAAASAAPSAAPSAAPSAGGSGAGAAARPGLLGAGAHPEEAQFSREELCRAAGSSVEA
                     LAELESFGLMSPRGAGERGSWYNGEDLLLLRVARDLSGYGIEARHLRMYKTFAEREAS
                     LIEQVVAPLVRQRNPDARSRARETIEALAELGSRMRDLALRAAVQGLADGHDFHR"
     gene            2738..4093
                     /locus_tag="VF995_03360"
     CDS             2738..4093
                     /locus_tag="VF995_03360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX9342639.1"
                     /translation="MSRRRGRSRGLTLGLSLIFTIAVTLSEPAGVLASSPLHAVASSP
                     AAPAAGSPATVALSGEAAASADQSVPGPVAPPTISAHAVLLADEATGQVLYERNPRLR
                     LPMASTTKVMTALLTLERVPQSRRVVVGSEPIKVGDDTLGLRVGERLSVHQLLLALLV
                     KSANDSAVALADAVDGTQAAFVARMNRRAAALGLTDTHYVTPYGLDRPGHQTSARDLA
                     RLWEVAMRRADFRALVATRAARIPGGGPLRSFVTTDKLLGSYPWIVGGKTGFTNKAGR
                     CLVASASRGGRRLVAVVLGAPDAFVAERALFDYGFDQFLWVRVARRGQTVPVSLGSGS
                     DTQPQPYQAAADVDALVRRDLLGAIHIDPASSGPAASTTPTTAKAEPGGSVRLWLSAG
                     AIPLAPLPLQVPPAAKAAAPPGAGAGSGSGTAGAAFTPGLVPAGSRGSTVDPLLQRAA
                     A"
     gene            4090..4704
                     /gene="hpt"
                     /locus_tag="VF995_03365"
     CDS             4090..4704
                     /gene="hpt"
                     /locus_tag="VF995_03365"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX9342640.1"
                     /translation="MTAGTGPAGPTAGGSDQPAVRTFLDRAALRARVAELGAQLRADY
                     HGLDPILVTVLRGAVIFLADLVRAAAMPCRVDFMAISRFDAPPTPETGVVRILKDLKL
                     DVSGVHVLVVEDVVDTGRTLAYLLKILAAREPASLRVCTLLDRRSRRAVEVPLAYVGF
                     GAPDAFLFGYGLDLDERERGRDEILLAADPEALRRDPSLVPPAH"
     gene            4820..5293
                     /locus_tag="VF995_03370"
     CDS             4820..5293
                     /locus_tag="VF995_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX9342641.1"
                     /translation="MVELTLVGVRIELPTQTPIVLLKERDGERFLPIWIGNVEATAIA
                     FALQGVATARPLTHDLLKNLLDELLVSVERVVITELKEGTFYATIELRQNGSRFSVSS
                     RPSDAIALAVRANTPIFAEESVLTEASILIKSDDEEREVEKFREFLDQVSPEDFQ"
     gene            5520..6095
                     /locus_tag="VF995_03375"
     CDS             5520..6095
                     /locus_tag="VF995_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9342642.1"
                     /translation="MLPMGGRHSSSPVPAEPGYRGPTVCRLVDITYRQLDYWARTGLV
                     TPSVRAAEGSGSQRLYSFTDVVELRIIKRLLNAGVSLQRIREAITYLRQEFKGRFSDV
                     TLMCDGPKRVYVCHSPNEIVDIFQKGQGVFGIAVGAVWADTAGDVAHLPDDQQEHEHE
                     PEHERAAGDRAAGDTGDTGEPGDPPRARQRA"
     gene            6265..7638
                     /gene="gcvPA"
                     /locus_tag="VF995_03380"
     CDS             6265..7638
                     /gene="gcvPA"
                     /locus_tag="VF995_03380"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX9342643.1"
                     /translation="MSYQPHTDADVRRMLDTLGLVDVDELFATIPADLRAPSGLDLPR
                     PHAEQEVAAELSRLAGRNRHLDELTCFLGAGVYDHYVPAVVWYLAGRGEFATSYTPYQ
                     PEMSQGVLQALFEYQTLISELTGLAVSNASLYDGASAVAEAATMAVAATGRGELLVSA
                     AVAPRVRELLETYGHGPGAKLVEIPALGGHTNLAEAAELAGEQTAAVIVAQPNFFGVI
                     EDAAAAAALAHRAGARLVVSFDPLAAGLLEPPGRLGADIVVGEGQALGNHPSFGGPAF
                     GFLACAQQDVRRLPGRLVGQTVDSDGQRAFVLTLQAREQHIRRERATSNICTNQTLNA
                     IAAAVYLAWLGPDGLDDLSHLCLRLARYAADRLTALPGVRLAFPGQPFYKEFTLRLPA
                     DPAEVCRRLADDHGLLAGLPLAGTVPGLDDGLLVAVTERRSKADIDHLADAMGSVLTD
                     LEKEGAA"
     gene            7635..>7839
                     /locus_tag="VF995_03385"
     CDS             7635..>7839
                     /locus_tag="VF995_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342644.1"
                     /translation="MSAAAAANPDGPAFEPAPWAAGTPGEREPERLIFERSAPGRRAS
                     SFPDGADLPDIDVDLAAVLPAGAR"
BASE COUNT         1142 a         3053 c         2636 g         1008 t
ORIGIN      
        1 accgcaccgc ccacgtgatc gtcaacggcg agctggtcga cgggctcggc ggcggggtct
       61 gccaggccgg caccacgatg ttcaacgcgg cgctgttcgg cggcctgcca gtgctggagc
      121 gccgcaacca cagcctccac atcagccact acccgctcgg ccgggacgcg accctcaact
      181 ggcccgacac cgacctgaag ttcaagaacg actcccccta cgggatctac atcacctcga
      241 cgtggagcgc ctccagcctg accttcacgc tgtggtccag ctcccggcac taccaggtgt
      301 cgctggccac ctcggcggaa agcaacttcc gcgacccacc caccaaggtc gaggacgacc
      361 cgagcctgcc ggcgggccag cagcagccca aggagaccgg ctcggccggg ttcgacgtca
      421 ccgtcacccg cacggtgacg ctgagtgggc agctcgtgcg gcgcgaccag ttcacctcgc
      481 gctacgatcc ctggccgacg atcatcagcc gcgggaccgg ccagccggca ccagccacca
      541 cggtgccggc cacggccatc gccacgggca cgtcggccta gccggcgaga gcgaccaacc
      601 gacacaaggg gcaggagcgg acatgtaccc agaggacctg aagtacacgc gtgagcacga
      661 gtgggccagg atcaagcgcg gccgcgccac catgggcatc accgactacg cgcaggacgc
      721 gctcggtgac atcgtctacg tcgacctccc cgcggtcggc gccacggtca ccaacggcga
      781 ggcgctcggt gaggtcgagt ccaccaagtc ggtctcggag ctgttcagcc cggtgtccgg
      841 cacggtcacc gaacgcaaca acgagctgga caagcacccc gagatcatca acgccgaccc
      901 ctacgggcag ggctggctgc tggtcatcga gctgagcgac ccggccgagg ccggccagct
      961 cctggacgcc gccgactaca gtaggctggt cgaggagagc tgagtcggcc gtgtcaacgt
     1021 ccggtcgagc ctagcgccgg atctcgatca actctcgatc agaccattgc gttgacccat
     1081 tttcagccgg tcatctacag tcagcgtcca tacaggagcg ccgcgcaggg ctcggacccc
     1141 aagcggcccg tcgtcgagcc caagagcccg gagggcagca tgttctgcac ccagtgcggt
     1201 cacaggaacc ccgacggggc ccacttctgc gcgaactgtg ggaccgcgct ggtgcctccc
     1261 cccgacgact ccaccaccac catcagcctg gccccccagg acctggaagc tgaggtggag
     1321 gacgaggtcg ccgccccagt cgaggacctg cgggagggga ccgcgatgct ggtggtccgc
     1381 cgcggcccga acgcgggcag ccgcttcctg ctcgacaagg aggtcgtgac cgccgggcga
     1441 cacccggaaa gcgacatctt cctggacgac atcaccgtct cgcggcgcca cgccgagatc
     1501 cgccgcaccc ccggcggctt caccgtgcat gacgtcggct cgctcaacgg cacctacctg
     1561 aaccgtgaac gggtcgagga cggcgagctg gtcggcggcg acgagctgca gatcggcaag
     1621 ttcaagctgg tgttcttcgc cggcgggcgg ccggggggcg gataggcgcg atgcgggatc
     1681 ccgtcgggcg cgatgccatg cggccaccac cagcttcagg tggcggcggg cagcgggagc
     1741 ggggggtgcc gtcccgcccg caccccagac gcgaccaggc caccggcccg gcagggacca
     1801 tgagcatcgg cgaggtgctc gcgatcctca agccggagtt ccccgacatc accgtctcca
     1861 agatccgttt cctggaggga gccgagctgg tgcggccggg ccgttctgcc tcgggctacc
     1921 gcatgttctc cgaggacgac gtcgcgcggc tgcgcttcgt cctgcgcgcc cagcgcgacc
     1981 agtacctgcc gctgcgggtg atccgccagc ggctcgccga gctcgatcag gtcggcggcc
     2041 tctacgcccg cggaggcacg ctggcgcacg gccccgagcc cggcggcgag gccagtccca
     2101 ccggtcccac tggcagcggc agcggcagcg gcagcgccgc cgccggcagg gccgggccgg
     2161 ccgcaggggg gcgccgcacc aggaccagga gcgccactgg cgccggcctg ccacctggtg
     2221 gcgccgctgc cgctgccagc gcggcgccca gcgcggcgcc cagcgcggcg cccagcgcgg
     2281 gcggctccgg cgccggagcc gcggcccgtc ccgggctgct cggcgcgggc gcgcatcccg
     2341 aagaggcgca gttcagccgc gaggagctgt gccgcgccgc cggcagcagc gtcgaagcgc
     2401 tcgccgagct ggagtcgttc gggctgatga gcccccgcgg cgctggtgag cggggcagct
     2461 ggtacaacgg cgaggacctg ctgttgctgc gggtcgcccg cgacctgtcc ggctacggca
     2521 tcgaagcgcg ccacctgcgc atgtacaaga ccttcgccga gcgggaggcg tccctgatcg
     2581 aacaggtcgt cgccccgctg gtgcgccagc gcaaccccga cgcgcgctcc cgcgcccgcg
     2641 agaccatcga ggcgctggcc gagctcggca gccgcatgcg cgacctggcc ctgcgcgccg
     2701 ccgtccaggg cctggccgac ggccacgact tccatcgatg agccggcgcc gcgggcgcag
     2761 ccgcgggctc accctcggcc tcagcctcat cttcaccatc gcggtcacct taagcgagcc
     2821 cgctggcgtg cttgcctcgt caccgctcca cgccgtcgcc tccagtcccg ccgcaccagc
     2881 ggctgggtcg ccggccacgg tggcgctcag cggcgaggcg gcggccagtg ccgaccagtc
     2941 ggtccccggg ccggtcgcac cgcccaccat cagcgcccac gcggtgctgc tggccgacga
     3001 ggccaccggg caggtgctct acgagcggaa cccgcggctg cgcctcccga tggcctccac
     3061 gaccaaggtg atgaccgcgc tgctcaccct ggaacgcgtg ccgcagagcc gccgggtcgt
     3121 ggtcggcagc gagccgatca aggtggggga cgacaccctg ggactccggg tcggggagcg
     3181 gctcagcgtc caccagctgc tgctcgccct gctggtgaag agcgccaacg actccgcggt
     3241 cgcgctcgcc gacgcggtcg acggcaccca ggccgcgttc gtggcgcgca tgaaccgccg
     3301 cgccgccgcc ctcggcctca ccgacaccca ctacgtcacc ccctacggcc tcgaccgtcc
     3361 cggccaccag accagcgccc gcgacctcgc caggctgtgg gaggtggcga tgcgccgggc
     3421 cgacttccgc gccctggtcg ccacccgcgc cgcgcgtatc cccggcggcg ggccgctgcg
     3481 cagcttcgtc accaccgaca agctgctcgg cagctacccc tggatcgtcg gcggcaagac
     3541 cggcttcacc aacaaggccg gccgctgcct ggtcgcctcc gccagccgcg gcggccgccg
     3601 gctggtcgcg gtggtgctcg gcgcgcccga cgcgttcgtc gccgagcggg ccctcttcga
     3661 ctacggcttc gaccagttcc tctgggtgcg ggtcgcccgc cgcggccaga ccgtcccggt
     3721 ctcgctcggc agcggcagcg acacgcagcc gcagccctac caggccgccg ccgacgtcga
     3781 cgcgctggtc cgccgcgacc tcctcggcgc catccacatc gaccctgcca gcagcggccc
     3841 ggcggcctcc accaccccaa ccaccgccaa ggccgagccg ggcgggtcgg tacggctgtg
     3901 gctcagcgcc ggcgccatcc ccctggcccc cctgccgctg caggtccccc cggcggccaa
     3961 ggccgccgcg ccgcccggcg ctggcgccgg ctccggcagc ggcaccgccg gagcggcctt
     4021 caccccaggc ctggtcccgg ccggctcgcg gggaagcacc gtcgatccgc tgctgcagcg
     4081 ggccgcggca tgaccgccgg gaccggcccg gccgggccga cggcaggcgg cagcgaccag
     4141 cccgccgtgc ggaccttcct ggaccgggcg gcgctgcggg cccgcgtcgc cgagcttggc
     4201 gcccagctcc gcgccgacta ccacggcctc gacccgatcc tcgtgacggt gctgcgcggc
     4261 gcggtgatct tcctcgccga cctggtccgc gcggcggcga tgccctgcag ggtcgacttc
     4321 atggcgatct cccggttcga cgccccaccg acaccggaga ccggcgtggt ccgcatcctc
     4381 aaggacctca agctggatgt ctccggcgtg cacgtgctgg tcgtcgagga cgtcgtcgac
     4441 accggccgga ccctcgccta cctgctcaag atcctggccg cgcgggagcc ggccagccta
     4501 cgggtctgca ccctgctcga ccggcggtcc cgccgagcgg tcgaggtgcc gctcgcctac
     4561 gtcggcttcg gcgcccctga cgcgttcctg ttcggctacg gcctggatct ggacgagcgc
     4621 gaacggggcc gcgacgagat cctcctcgcc gccgatcctg aggcgctgcg ccgcgatccc
     4681 agcctggttc caccagctca ctgaaggacc gctgggcgag cgccgggcgt gtcgcgcccg
     4741 tcgggagcgt ctgatagcct ctgagtgaga ggcgcccgac ccgggcgcag cgctgttcga
     4801 cggtcgaggg ggttgcgcgg tggtcgagct caccctcgtt ggtgtccgca tcgagctccc
     4861 cacccagaca cccatcgtgc tgctcaagga gcgcgacggc gaacgcttcc tgccaatctg
     4921 gatcggcaac gtcgaggcga ccgcgatcgc cttcgccctc caaggcgtcg ccaccgcgcg
     4981 gccgctcacc cacgacctgc tcaagaacct gctcgacgag ctgctcgtct cggtcgagcg
     5041 ggtggtgatc accgagctca aggaaggcac cttctacgcc accatcgagc tgcgccaaaa
     5101 cggctcccgg ttctcggtct ccagccgccc cagcgacgcc atcgccctcg cggtccgcgc
     5161 caacaccccg atcttcgcgg aggagagcgt cctcacggag gcctccatcc tgatcaagag
     5221 cgacgacgag gaacgcgaag tcgagaaatt ccgcgaattc cttgaccagg tcagccctga
     5281 ggatttccag taggaatcgc ctcggcatcc cttcagaggg aatttgcggg tccgggaaat
     5341 ggcgcgcctt cgttgacgat cagcggcttg atccctacgc tgaaggtcac atcgctgtaa
     5401 ttcgcatgcg accgtagggg gttgcgatga ccggacgccg gcctcccatg caggcgggcg
     5461 agagggatgc cgtccatggg aggccggcgg agccgccgag tgtgcaggcg ggcgaagcga
     5521 tgctgcccat gggaggccgg cacagctcct caccagtccc ggcggagccg ggctatcgcg
     5581 gcccgaccgt ctgccggctg gtcgacatca cctaccgcca gctcgactac tgggcccgga
     5641 ccgggctggt caccccctcg gtgcgggccg ccgaagggtc cggcagccag cgcctctaca
     5701 gcttcaccga cgtggtggag ctgcgcatca tcaagcggct gctgaacgcg ggcgtctccc
     5761 tgcagcgcat ccgcgaggcg atcacctacc tgcgccagga attcaaaggc cgcttcagcg
     5821 acgtcaccct gatgtgcgac ggccccaaac gggtctacgt ctgccactcg cccaacgaga
     5881 tcgtcgacat cttccagaag ggccagggcg tgttcggcat cgccgtcggc gcggtctggg
     5941 ccgacaccgc aggcgacgtc gcccacctcc ccgacgacca gcaggagcac gagcacgagc
     6001 cggagcacga gcgggcagcc ggtgaccgcg ccgccggcga caccggcgac accggggagc
     6061 cgggcgaccc gccgagggcg cgccagcggg cctgagcttg cgccgctcca acgccccgtc
     6121 cgttttgggc ggattccgtt gcgggatccg gaacgtcggt ggttgaatcg tccggttcca
     6181 gccaagcgaa acagatgaac aaatccctat ggcgacatgt cgacaaagcg acaggtcgac
     6241 ggtcgtctcg gaagagggga cagcgtgagc taccagccgc acaccgacgc cgacgtccgc
     6301 cgcatgctcg acaccctcgg gctcgtcgac gtcgacgagc tgttcgccac catcccggcg
     6361 gacctgcgcg ccccgtccgg cctcgacctg ccccggcccc acgccgagca ggaggtcgcc
     6421 gccgagctca gccgactcgc cggccgcaac cgccacctcg acgagctgac ctgcttcctc
     6481 ggcgccggcg tctacgacca ctacgtccca gcggtggtct ggtacctcgc cgggcggggc
     6541 gagttcgcca cctcctacac cccctaccag cccgagatga gccagggcgt gctgcaggcg
     6601 ctgttcgagt accagacgct gatcagcgag ctgaccggcc tggcggtctc caatgcctcc
     6661 ctctacgacg gcgcctccgc cgtcgccgag gccgccacca tggccgtcgc cgccaccggc
     6721 cgcggcgagc tgctggtctc ggccgcggtc gccccccgcg tgcgagagct gctggagacc
     6781 tacggccacg gccccggcgc caagctggtc gagatcccgg ccctcggcgg ccacaccaac
     6841 ctcgccgagg ccgccgagct ggccggtgag cagaccgccg cggtgatcgt cgcccagccg
     6901 aacttcttcg gcgtgatcga ggacgccgcc gccgccgccg cgctggccca ccgtgccggc
     6961 gcgcggctgg tggtcagctt cgacccgctc gccgccgggc tgctggaacc gcccggccgc
     7021 ctcggcgccg acatcgtcgt cggcgagggc caggcgctcg gcaaccaccc gagcttcggc
     7081 ggccccgcct tcggcttcct cgcctgcgcc cagcaggacg ttcgccgcct gcccggccgg
     7141 ctggtcggcc agaccgtcga cagcgacggc cagcgcgcct tcgtcctcac cctgcaggcc
     7201 cgcgagcagc acatccgccg cgagcgggcg accagcaaca tctgcaccaa ccagaccctc
     7261 aacgccatcg ccgccgccgt ctatctcgcc tggctcggcc ccgatggcct cgacgacctc
     7321 agccatctct gcctgcggct ggcccgctac gccgccgacc ggctcaccgc cctgcccggc
     7381 gtgcggctcg ccttccccgg ccagccgttc tacaaggagt tcacgctgcg cctgcccgcc
     7441 gaccccgccg aagtctgccg ccgcctcgcg gacgaccatg gcctgctcgc cgggctgccg
     7501 ctggccggga ccgtccccgg cctcgatgac ggcctgctgg tcgccgtcac cgagcggcgc
     7561 agcaaagccg acatcgacca cctcgctgac gcgatgggca gcgtcctcac cgacctggag
     7621 aaggagggcg cggcatgagc gcggcagcgg cagcgaaccc ggacggcccg gcgttcgagc
     7681 cggcgccgtg ggcggccggc acgccggggg agcgcgagcc ggagcggctc atcttcgaac
     7741 ggtccgcgcc aggccggcgg gcgtccagct tccccgacgg cgccgacctg cccgacatcg
     7801 acgtcgacct ggcggcggtc ctgccggccg gagcgcggc
//
LOCUS       DASWDC010000105        10181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1279190, whole genome shotgun sequence.
ACCESSION   DASWDC010000105 DASWDC010000000
VERSION     DASWDC010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10181
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279190"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VF995_03390"
     CDS             complement(<1..828)
                     /locus_tag="VF995_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX9342645.1"
                     /translation="MKERVVITGIGALSPLGLDAPSTWAAMLEGRSGIDTIAAFDPSP
                     FPVRIAGEVRGFNPETVVSRKDARRMDRGVLFAVAAADEAVADAGLDKDSLVPERTGV
                     VVGSAIGGVALIEQQQKILLERGPDRVSPTWLPNALVDTATSYIATRFGAIGCNFAVV
                     SACATGSHAIGEAADLIRRGRADVFVAGGTEACITPLVLAGFCQMQALAPRNDEPSAA
                     SRPYDRARSGFVVSEGAAVVVLESEAHARARGAKVYAEVAGYGASNDAYHVATPHPES
                     "
     gene            complement(838..2367)
                     /locus_tag="VF995_03395"
     CDS             complement(838..2367)
                     /locus_tag="VF995_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018721833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX9342646.1"
                     /translation="MGDRRVLALDLGTSSVRALLFDGAGRALPGMLARRATELEIADD
                     GKAELDPEAVVAALCDCLDELARQGYLDGVTDVAISCAWHSVIAADAAGRPLTGALTW
                     ADTRAAGMVKEVRARADVERLHAVTGTLPHTTYWTVKIPWLARNCNPAAERAGEPSNA
                     VHGRPAEAPARYLGLSEYATRALTGEAGASVSMASGTGLLDLASMTWDPEALGLAGVD
                     EAAVPGLLEEGWRGRLIPELARRWPALAGAAWHPPSGDGAASNVGAGCVTPDQVAINV
                     GTSAAVRAVHDDGDAREGGPLGRALWRYRVDQDRLVTGAAYSGGGNLYAWARKLLALP
                     ADEQVEAALWTVPPGAEGVQVMPYHAGSRAPLDLVAGSGVIAGLSLATTAVEVVAASL
                     EAVCFQLAAGVEALAASLARPDFEMVASGGAIVASRWWQQTLANVLDRPVRVVDEPEA
                     SARGAALLALGLAGGEGARGEGVAAPTTRHVVAPDPAGVKAERAARAAHQRLADCLGY
                     T"
     gene            complement(2409..4664)
                     /locus_tag="VF995_03400"
     CDS             complement(2409..4664)
                     /locus_tag="VF995_03400"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HEX9342647.1"
                     /translation="MLRNAPIRSKFILTLIVPLLALTVVTLLGLRASLAEGARAGHVN
                     QLARFADGLAPVVHELQNERSLSRNVIGSSRASGASILRAQRRVTDQAAASYRTAASR
                     LRLGPGDSGLRLRIDYAFGELAKLTRQRELIDRSPIRGDELADQPLLEQAELEGNVHL
                     GEGHPPVTNLQQALDQYSDTVNDLLDINGEIVPGSSGQRLLDSMAGSVALGRAMDFVD
                     LQRGFLFGLLSQHRTALGDTEHGRLTVLMAAESIYLAQFQNHATPALRQLLNQTVTGP
                     QVAQVAAVEREAALRERFPGGLTPGAWYSAASANLDRLRQVELAQNHDIVTTSAAVVA
                     SAGRRALLYSLLLVTALVLAVGLSLISARSMIVPLSRLKVAADEIAERTLPSVVRRLR
                     DGEEVDLAAESAGPVAVDSSDEIGQLAESFNAVHQVAVQLAGKEAALRRSIGEMFLNL
                     ARRSQSLLDRQLELLRAVPLTGPAELPGAVAELDHLASRMRRNAENLIVLSGADPSRR
                     WRGPVPVTELLAAAVAEINDHTRVELLSLPDAPIAGHAAADVMHLLAELIENAVTFSA
                     PDTRALVAGQLLPAGYLLEIEDQGIGMSDDQLVDANQRLAQPPEVDLAQARLLGFFVV
                     GHLAARHGMKVQLRHSWSGGITALVLLPADLLARQPVHGQPDRWGIAAARESAPTGVD
                     PLGTPNGNGLGAPSSPGRRAQARTFAAADAVADGELDERALAELGLGPGWFDSRIPSV
                     HAPLRRQPPGE"
     gene            4912..5565
                     /locus_tag="VF995_03405"
     CDS             4912..5565
                     /locus_tag="VF995_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEX9342648.1"
                     /translation="MAEDIEAVRGTGDPTRAVTPPVDPAAGAIADPAPLGLAGFALTT
                     FVLSMFNAGLVGKAGEPVVLGLALAYGGGAQLLAGMWEFRKGNTFGATAFSSFGAFWI
                     SYWVFVAFFAKPGTPQAVPATAVGLYLIAWGIFTFYMWIASFRTTAAVNVVFLLLWVT
                     FVLLGIGAAGGHSSITKLGGWVGLATAVAAWYASFAGVANWTFGREMVPVRALRRAA"
     gene            complement(5624..7816)
                     /locus_tag="VF995_03410"
     CDS             complement(5624..7816)
                     /locus_tag="VF995_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX9342649.1"
                     /translation="MSLSWSWRERIKPWHRPIPRISRELLGAWLELFVLTGFAIAQPI
                     LDVTGEAPDFFLFRRASRLDMLLLVLAVTIAPALVLGLVELAAELAGERARELVHLVM
                     VAGLSALIALEALKKLLPMRGKRLALLALLLAAGFALLYQKGPWVRLWLRYLVPAPLV
                     FALLFATTSPSAELLRPVRTAAVAPLRTASGRALPPIVMVFFDEFPLQSLLDSSGQVD
                     RRVYPNFADFAAHSTWYRNATGVSGFTPYAVPAMLTGRYPSKVVAPILQNYPENLFTL
                     FGSWYNLKVSETITQLCPPVRCGVTAGSRDASGLGAVLKDSAGIYKSIVSPYDTPVNP
                     ASFADTQTAAEAGGKGGKKLDTKDPSALFKNNLSNAELNAPSRFTDFLDSIKASDAQP
                     TLYFLHILMPHTPWRFEPDGALYNQGSLPFLLSLPEEQWPRQYVELTHERHLLQLAYT
                     DKLVGDLIQRLKDQGLYDKSLVLMTADHGNGFATGERARRLGPSNAPSLMWVPLLVKA
                     PNQHAGKVDDRNWEQVDLLPTLADMAGIKVPWKMDGLSEVGPPARTRGDKYWYDHPGD
                     RVSRDGPSNWATVLKGVTDSLVRAHQHGDQGFYQYGWFADWVYKPPSAIGRVAARPAA
                     KATIRNWKLFATIDPKAATVPALVVGQLTTGVPPPGMTVLVAVNGRVGGASLFYADHA
                     GQPADKFAVIVPDFLYKRGPGQSQLQVYLVKDGGGQVPKRLLPVALSG"
     gene            complement(7830..9254)
                     /locus_tag="VF995_03415"
     CDS             complement(7830..9254)
                     /locus_tag="VF995_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX9342650.1"
                     /translation="MPFAVEPGSFRDPAGSVFYLDGRVLRGFDPTGWADFEALAATRF
                     WQRATERGELVKTTAVELATLPEATTKLTGIAGVVEHERIPVVSYPFEWTFSMLRAAA
                     LCHLDLLLAGLAEGFTMKDGYAYNLQFRGARPVFIDVGSFTRVQPGEPWAGYRQFCQT
                     MLYPLLLQAHRGVRFQPFLRGAVQGITPQDARGLLRGAGRRAGRRAGVLKHVVLHQAI
                     DARMSGARASDTRKELAAAGFSAELQAATVKAIRKLVAKLSWSEAGKPSTWSDYQRTS
                     TYTDAERERKEAFVERSLAAAAPLGICADLGGNDGTYARIAQRHASYVVTADADAVTV
                     DRLWQRLAAEGNERILPLVVDLTDPSPGLGWRGTERGPLFERLRPDAVLALALVHHLA
                     ISGNVPLPALVDWWHGLGGRLVVEFVDPKDPMADRLLANKPAGMHGDYRREVFERLLE
                     QRFEVLDREELPSGTRMLYHAVPR"
     gene            complement(9260..9433)
                     /locus_tag="VF995_03420"
     CDS             complement(9260..9433)
                     /locus_tag="VF995_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342651.1"
                     /translation="MRSLAYLDAMSGSMVVQAVVAGVAGIAVFFKLFWRRLTAPFRGR
                     RAAQVEMAESDER"
     gene            9689..>10181
                     /locus_tag="VF995_03425"
     CDS             9689..>10181
                     /locus_tag="VF995_03425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9342652.1"
                     /translation="MNTNNAGIEIRPIAAEEFPAYCQVPANAFGQTLTPERVKDWSEG
                     FELDRTLAAFDGDRIVGTGGADSLELTLPGGTVIPVGGLTAISVLPTHRRRGILRAIM
                     ERHFQDVEARGEVVSALLASESAIYSRFGYGPATYGADLEIDPRSSAFLRPPPGGGRA
                     RLLD"
BASE COUNT         1441 a         3612 c         3712 g         1416 t
ORIGIN      
        1 gctctccggg tgcggggtgg cgacgtggta ggcgtcgttg gaggcgccgt agccggcgac
       61 ctcggcgtag accttggcgc cgcgggcgcg ggcgtgcgcc tcggactcga gcaccaccac
      121 cgccgcgccc tcgctcacga cgaatcccga ccgcgcccgg tcgtagggcc gcgacgccgc
      181 cgagggctcg tcgttgcggg gcgcgagcgc ctgcatctgg cagaacccgg ccagcaccag
      241 cggggtgata caggcctcgg tgccgccggc gacgaacacg tcggcacggc cgcgccggat
      301 caggtcggcc gcctcgccga tcgcgtgcga gccggtggcg caggccgaca ccaccgcgaa
      361 gttgcagccg atcgccccga accgggtggc gatgtagctg gtcgcggtgt cgaccagggc
      421 gttcggcagc caggtcgggg agacccggtc gggcccgcgt tccagcagga tcttctgctg
      481 ctgctcgatc agggcgaccc cgccgatcgc cgagccgacc accaccccgg tccgctccgg
      541 caccagcgag tccttgtcca ggccggcgtc ggcgaccgcc tcgtcggcgg cggcgaccgc
      601 gaacagcacg ccccggtcca tccgccgggc gtccttgcgc gaaaccaccg tttccgggtt
      661 gaagccgcgc acctccccgg cgatcctgac cgggaagggc gacgggtcga aggcagcgat
      721 cgtgtcgatg ccgctgcgtc cctccagcat cgccgcccag gtcgaggggg cgtccagccc
      781 gagcgggctg agcgcgccga ttccggtgat caccacacgt tccttcataa acgaagccta
      841 cgtgtacccg aggcagtcgg ccagtcgctg gtgggcggcg cgggcggcac gctcggcctt
      901 gacccccgcc gggtcggggg cgaccacgtg cctggtggtg ggcgcggcca cgccctcccc
      961 cctggcgccc tctccgccag cgaggccgag ggcgagcagg gccgcgcccc gcgccgacgc
     1021 ctccggctcg tccaccaccc ggacggggcg gtcgaggacg ttggcgaggg tctgctgcca
     1081 ccagcgcgac gcgacgatcg ccccgcccga ggcgaccatc tcgaagtccg ggcgcgcgag
     1141 gctcgccgcc agcgcctcga ccccggcggc gagctggaag cagaccgcct ccaggctggc
     1201 ggcgaccacc tcgacggcgg tggtagccag cgacagcccg gcgatcaccc ccgacccggc
     1261 gaccaggtcc aggggcgccc gcgacccggc gtggtagggc atgacctgga cgccctcggc
     1321 accgggcggg accgtccaca gggcggcctc gacctgctcg tccgccggga gggcgagcag
     1381 cttcctggcc caggcataga ggttgccccc gccggagtac gccgccccgg tcaccaggcg
     1441 gtcctggtcg acccggtagc gccacagcgc ccgtcccaga ggaccgcctt ccctggcgtc
     1501 accgtcatcg tggaccgcac gcaccgccgc cgaggtgccg acgttgatcg ccacctgatc
     1561 gggggtgacg cagcccgcgc cgacgttgga ggcggccccg tcgccgctgg gcgggtgcca
     1621 ggcagccccg gccagcgccg gccagcgccg cgccagctcc gggatgagcc tgccgcgcca
     1681 gccctcctcc agcaaccccg gcacggccgc ctcgtccacg ccggcgagcc cgagcgcctc
     1741 cggatcccag gtcatcgagg ccaggtccag caggccggtc ccggacgcca tcgacaccga
     1801 agcgcccgcc tcgccggtca gcgccctggt ggcgtactcg ctcagcccga ggtagcgggc
     1861 gggcgcctcc gccggcctcc cgtggacagc attgcttggc tcgcccgcac gctcggccgc
     1921 cggattgcag ttgcgcgcca gccaggggat cttcacggtc cagtaggtgg tgtgcggcag
     1981 cgtcccggtg acggcgtgca ggcgctcgac gtccgcccgc gcccgcactt ccttgaccat
     2041 ccccgccgcc cgggtatcgg cccaggtgag cgccccggtg agcggcctgc cggcggcgtc
     2101 cgccgcgatc accgaatgcc acgcgcagga gatcgccacg tctgtcacac cgtcgaggta
     2161 gccttgccgg gccagctcgt ccaggcagtc gcaaagcgcc gcgaccaccg cctccgggtc
     2221 cagttccgcc ttgccgtcgt cggcgatctc aagctccgtc gcccgccgcg ccagcatccc
     2281 tggaagcgcc cgccccgcgc cgtcgaacag cagcgcccgc accgacgagg tccccaggtc
     2341 gagcgcgagc acgcgccggt cgcccaccgt ccacctcctc ccccggtagc ttccccaggg
     2401 gcctcaagct actcgccggg aggctggcgg cgcaggggcg cgtggacgct cggaatgcga
     2461 ctgtcgaacc agccgggacc gaggccgagt tcggcgagcg cgcgctcgtc gagctcgccg
     2521 tcggcgacgg cgtcggcagc ggcgaaggtc cgcgcctggg cgcggcggcc gggggagctg
     2581 ggcgcgccca atccgttgcc gttgggggtg ccgagcgggt cgacgccggt cggggcggat
     2641 tcccgggcgg cggcgatgcc ccagcggtcc ggctggccgt ggaccggctg gcgggcgagc
     2701 aggtcggcgg ggaggaggac cagggcggtg atgccgccgg accaggagtg gcgcagctgg
     2761 accttcatgc cgtggcgggc ggcgaggtgg ccgacgacga agaagccgag gaggcgggcc
     2821 tgggcgaggt cgacctcggg gggctgggcg aggcgctggt tggcgtcgac gagctggtcg
     2881 tcgctcatgc cgatgccctg gtcctcgatc tccaacaggt agccggcggg gaggagctgg
     2941 ccggcgacca gcgcccgggt gtcgggggcc gagaaggtga cggcgttctc gatcagctcg
     3001 gcgagcaggt gcatcacgtc ggcggcggca tgcccggcga tcggcgcgtc cggcagcgac
     3061 agcagctcga cccgggtgtg gtcgttgatc tcggcgaccg cggcggcgag cagctcggtc
     3121 acggggaccg ggccgcgcca gcggcgtgag gggtcggcgc cggacagcac gatcaggttc
     3181 tcggcattgc gccgcatgcg gctggccagg tggtccagct cggcgacggc gccgggcagc
     3241 tcggcgggcc cggtcagcgg gacggcgcgg agcagctcca gctggcggtc gagcaggctc
     3301 tggctgcgcc gcgccaggtt gaggaacatc tcgccgatgc tgcgccgcag cgcggcctcc
     3361 ttgccggcca gctgcacggc gacctggtgg acggcgttga aggactcggc gagctggccg
     3421 atctcgtcgg aggagtccac cgcgaccggg ccggccgact cggcggcgag gtcgacctcc
     3481 tcgccgtccc gcaggcggcg gaccacgctg ggcagcgtgc gctcggcgat ctcgtcggcg
     3541 gcgaccttga gccgggacag cggcacgatc atcgatcggg cactgatcag cgagaggccg
     3601 accgcgagta ccagcgcggt gaccagcagc aggctgtaga gcagcgcgcg ccggccggcg
     3661 ctggccacca ccgcagcgct ggtggtgacg atgtcatggt tctgcgccag ctccacctgc
     3721 cggagccggt cgagattggc gctggcggcg ctgtaccagg ccccgggggt gaggccgccg
     3781 gggaagcgct cccgcagcgc tgcctcgcgc tcgacggcgg cgacctgggc gacctgcggg
     3841 ccggtgacgg tctggttgag gagctggcgc agcgcggggg tggcgtggtt ctggaactgc
     3901 gccaggtaga tgctctcggc ggccatcagc acggtcagcc ggccgtgctc ggtgtcgccg
     3961 agggcggtgc ggtgctgcga cagcaggccg aacaggaaac cgcgctgcag gtcgacgaag
     4021 tccatcgcgc gccccagcgc caccgacccg gccatcgagt cgagcagccg ctggccgctg
     4081 gagcccggga cgatctcgcc gttgatgtcc agcaggtcgt tgaccgtgtc gctgtactgg
     4141 tcgagcgcct gctgcaggtt ggtgacgggc gggtggccct cgccgaggtg gacgttgccc
     4201 tccagctccg cctgctcgag cagcggctgg tcggccagct cgtcgccccg gatcgggctg
     4261 cggtcgatca gctcgcgctg gcgggtgagc ttggcgagct cgccgaaggc gtagtcgatg
     4321 cgcaggcgca ggccgctgtc gcccgggccc aggcggagcc gggaggcggc cgtgcggtag
     4381 ctggccgccg cctggtcggt gacccggcgc tgcgcccgca ggatcgaggc gccggaggcg
     4441 cggctggagc cgatgacgtt gcgcgacagg ctgcgctcgt tctgcagctc gtggaccacc
     4501 ggggcgaggc cgtcggcgaa gcgggcgagc tggttcacgt gcccggcgcg ggcgccctcg
     4561 gccaggctgg cccgcaaccc gagcagggtg accacggtca gtgccagcag cgggacgatc
     4621 agggtgagga taaatttcga ccggatcggc gcgtttcgta acatcagccg gccccttcca
     4681 atgaggggca agacgctatc gtccacgctt gataagggca aacgcgcacc cgaagccgct
     4741 caccagccgc aagcggggca agcgtcgctt tcgttcccaa tacttgcccc gctgcctttc
     4801 cggcagaagc cctctatagg tttcggggga atcttgaccg gatgtgagat gaagcgcacg
     4861 atccgagcac aggcggaatt ccgcgtgccg ttgcgcaagg gagggcttcc gatggcggaa
     4921 gacatcgaag ctgtgcgggg gaccggcgac ccgaccaggg cggtaacgcc tccagtggac
     4981 ccggcggccg gggcgatcgc cgacccggcc ccgctcggcc tggcaggctt cgcgctgacc
     5041 accttcgtcc tcagcatgtt caacgccggc ctggtcggca aggcgggcga gccggtcgtc
     5101 ctcggcctgg cgctcgccta cggcggcggc gcccagctcc tcgccgggat gtgggagttc
     5161 cggaagggca acaccttcgg cgccaccgcg ttcagctcgt tcggcgcctt ctggatctcc
     5221 tactgggtgt tcgtggcctt cttcgccaag ccgggcaccc cgcaggccgt cccggcgacc
     5281 gccgtgggcc tgtacctgat cgcctggggg atcttcacgt tctacatgtg gatcgcctcg
     5341 ttcaggacca ccgccgccgt gaacgtggtg ttcctgctgc tgtgggtcac tttcgtcctg
     5401 ctcggtatcg gcgcggccgg tggccacagc tcgatcacca agctgggcgg ctgggtcggc
     5461 ctcgccaccg cggtcgcagc ctggtacgcc tccttcgccg gcgtcgccaa ctggaccttc
     5521 ggccgcgaaa tggtcccggt gcgcgccctt cgccgcgccg cctgatccca ggccacgcgg
     5581 tcactggcag cgcgggaagg aacaggctcc cgcgctgcca gcctcaaccg gagagggcga
     5641 ccggcaggag ccgcttcggg acctggccgc cgccgtcctt gaccaggtag acctggagct
     5701 gggactggcc gggtccccgc ttgtagagga agtcggggac gatcaccgcg aacttgtcgg
     5761 cgggctggcc agcgtggtcg gcatagaaca gcgacgcccc gccgacccgg ccgttgaccg
     5821 ccaccagcac ggtcattccc ggcggtggca ccccggtggt gagctggccg acgaccaggg
     5881 ccgggacggt cgccgccttc gggtcgatgg tcgcgaacag cttccagttc ctgatggtcg
     5941 ccttggcggc tggccgcgcg gcgacccgcc cgatcgcgct gggcggcttg tagacccagt
     6001 cggcgaacca gccgtactgg tagaagccct ggtcgccgtg ctggtgggcc cgcaccagcg
     6061 agtcggtcac gcccttcaat acggtcgccc agttcgacgg cccgtcgcgg ctcacgcggt
     6121 cgccgggatg gtcgtaccag tacttgtcgc cgcgggtccg ggccggcggg cccacctcgg
     6181 agaggccgtc catcttccag gggaccttga tgccggccat gtcggcgagg gtcgggagca
     6241 ggtcgacctg ctcccagttg cggtcgtcca ccttgccggc atgttggttc ggcgccttga
     6301 cgagcagcgg cacccacatc agcgacggcg cgttgctggg gccgaggcgg cgggcacgct
     6361 cgccggtggc gaagccgttg ccgtggtcgg cggtcatcag caccagggat ttgtcataca
     6421 ggccctggtc cttgagccgc tggatcaggt cgccgaccag cttgtcggtg taggcgagct
     6481 gcagcaggtg gcgctcgtgg gtcagctcga cgtactgcct gggccactgc tcctcgggga
     6541 ggctcagcag gaacggcagg ctgccctggt tgtagagggc cccgtcgggc tcgaagcgcc
     6601 acggcgtgtg cggcatcagg atgtgcagga agtacagcgt cggctgcgcg tcgctggcct
     6661 tgatcgagtc caggaagtcg gtgaagcgcg acggggcgtt gagctccgcg ttgctgaggt
     6721 tgttcttgaa gagggcggag gggtccttgg tgtcgagctt cttcccgccc ttgccgcccg
     6781 cttcggcggc ggtctgggtg tcggcgaacg aggccgggtt caccggggtg tcatagggcg
     6841 agacgatact tttgtagatg ccggccgagt ccttgaggac cgcgcccagg ccggaggcgt
     6901 cccgggagcc ggccgtgacg ccgcagcgga ccggcgggca aagctgcgtg atcgtctccg
     6961 agaccttcag gttgtaccag ctgccgaaga gggtgaagag gttctcgggg tagttctgca
     7021 ggatcggcgc caccaccttg gaggggtagc ggccggtgag catcgccggg accgcgtacg
     7081 gcgtgaaccc cgacaccccg gtggcgttgc ggtaccacgt ggagtgcgcg gcgaagtccg
     7141 cgaagttggg gtagacgcgc cggtcgacct ggccgcttga gtccagcagc gactgcagcg
     7201 ggaactcgtc gaagaacacc atcacgatcg gcggcagcgc gcggcctgag gccgtccgca
     7261 gcggcgccac cgcggcggtc ctgaccgggc gcagcagctc ggcgctcggc gaggtggtgg
     7321 cgaacagcag cgcgaacacc agcggcgcgg gcaccaggta gcgcagccac aaccgcaccc
     7381 aggggccttt ctggtagagc agggcgaacc cggcggccag cagcagcgcc agcagcgcca
     7441 gccgcttgcc ccgcatcggg aggagcttct tcaacgcctc cagcgcgatc agcgcggaca
     7501 gcccggcgac catcaccagg tggaccagct cgcgggcccg ctccccggca agctcggcgg
     7561 ccagctcgac cagaccgagc acgagcgccg gcgcgatcgt gacggcgagc accagcagca
     7621 gcatgtcgag gcggctcgcc ctccggaaga gaaagaaatc cggtgcttcg ccagtgacgt
     7681 cgaggatcgg ctgggcgatc gcgaagccgg tcagcacgaa cagttccagc catgccccga
     7741 gcagctcacg ggagatacgg gggatgggac ggtgccaggg tttgatccgc tctcgccagc
     7801 tccagctcag gctcattcgg ggaccgggct catctgggga ccgcgtgata gagcatccgg
     7861 gtgccgcttg gcagctcctc gcggtccagc acctcgaagc gctgctccag caggcgctcg
     7921 aagacctcgc ggcggtagtc gccgtgcatg ccggcgggct tgttggccag cagccggtcg
     7981 gccatcgggt ccttcgggtc gacgaactcg accaccagcc ggccgccgag gccatgccac
     8041 cagtccacca gggccggcag cggcacgttg ccgctgatcg ccaggtgatg gacgagggcg
     8101 agcgccagca ccgcgtcagg gcgcagccgc tcgaacagcg ggccccgctc ggtccctcgc
     8161 cagccgaggc cgggcgaggg gtcggtcagg tccacgacga gtggcaggat ccgctcgttg
     8221 ccctcggcgg cgaggcgctg ccagaggcgg tcgacggtga ccgcgtcagc gtcggcggtc
     8281 accacgtagc tggcgtggcg ctgggcgatc cgggcatagg tgccgtcgtt gccgccgagg
     8341 tcggcgcaga tcccaagcgg cgcggcggcg gccagggagc gctccacgaa cgcctccttg
     8401 cgctcccgct cggcgtcggt gtaggtgctg gtccgctggt agtccgacca ggtggagggc
     8461 ttgccggcct cgctccagga gagcttggcg accagcttgc ggatcgcctt gacggtggcg
     8521 gcctgcagct cggcggagaa accggcggcc gcgagctcct tgcgggtgtc tgaggcacgg
     8581 gcgccggaca tgcgcgcgtc gatcgcctgg tgcagcacca cgtgcttgag caccccggcc
     8641 ctgcgcccgg ccctgcgccc ggcgccgcgc agcagcccgc gggcgtcctg gggggtgatg
     8701 ccctgcaccg cgccgcgcag gaacggctgg aagcggacgc cccggtgcgc ctggagcagc
     8761 agcgggtaca gcatcgtctg gcagaactgc cggtagcccg cccacggctc gcccggctgc
     8821 acccgcgtga acgagccgac gtcgatgaac accggccgcg cgccgcggaa ctgcaggttg
     8881 taggcgtagc cgtccttcat cgtgaagccc tcggccagcc cggccagcag cagatccagg
     8941 tggcacaggg ccgccgcccg gagcatcgag aaggtccact cgaacggata ggacaccacc
     9001 gggatgcgct catgctcgac gaccccggcg atgccggtca gcttcgtggt cgcctccggc
     9061 agggtggcca gttcgaccgc ggtggtcttg accagctcgc cgcgctcggt cgcccgctgc
     9121 cagaaccgcg tggcggccag cgcctcgaag tccgcccagc ccgtcgggtc gaacccgcgc
     9181 agcacacggc cgtccaagta gaacaccgat cccgccgggt cgcggaacga gcccggctca
     9241 accgcgaacg gcatccccgc taccgctcgt cggactcggc catctcgacc tgggctgccc
     9301 ggcgcccacg gaacggggcc gtcaggcgcc gccagaacag cttgaagaac acggcgatcc
     9361 ccgcgactcc cgctacgacc gcctgaacaa ccatgctgcc ggacatggcg tcaagatatg
     9421 ccaagcttct catccggggc gtttctcctt attgctgggc tgatccacgt gtcctgcccc
     9481 gcaacctgtt tcggcgggga aaggcacgct cacgggtggc actacgaaac gatgcgttca
     9541 ggtcaggatg gcgacagacg atatcacgac cctccacaga accgtcattc atcatccatg
     9601 ccgtattcac gttcagctga caaaaccggc cgccaaactg tgggaaattc gcgtgtgccg
     9661 gcacgcgaga tccatgacaa tcggcgtcat gaataccaac aacgccggga ttgagatccg
     9721 gccgattgcc gctgaggagt tccccgccta ctgccaggtg ccagccaacg ccttcgggca
     9781 gaccctgacg cccgaacggg tcaaggactg gtccgaaggc ttcgagctcg accgcacctt
     9841 ggccgcgttc gacggtgacc gcatcgtcgg caccggcggt gccgactcac tggagctgac
     9901 cctgccgggc gggaccgtca tcccggtcgg cggcctgacc gcgatctcgg tgctgccgac
     9961 ccaccgccgc cgcggcatcc tccgcgccat catggagcgc cacttccagg acgtcgaggc
    10021 tcgcggcgag gtcgtctccg cgctgctcgc ctcggagtcg gccatctaca gccgcttcgg
    10081 ctacggcccg gcgacctacg gggccgacct ggagatcgac ccgcgctcaa gtgccttcct
    10141 gcggccgccc cccggaggcg gccgggcgcg cctgctggac c
//
LOCUS       DASWDC010000106         4294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1612703, whole genome shotgun sequence.
ACCESSION   DASWDC010000106 DASWDC010000000
VERSION     DASWDC010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612703"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VF995_03430"
     CDS             complement(<1..1026)
                     /locus_tag="VF995_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like protein"
                     /protein_id="HEX9342653.1"
                     /translation="MPVSRPVPRYPGVTTTTTRPPLPIDSREQLVYLLTQACELEQGV
                     LCEYLFALYSLKRDPHCQEGLSAEQAAQVTRWGQTLSQIAVQEMLHLTLATNLLTAVG
                     ATPHFHRPNFPISCRWYPPDVRIALAPFGEAALRHFLYLERPDHIDVEDADAFAAACE
                     CRPLTATPTTMMAAPQDYSTVGHLYRSIEHGFSRLVERYGEAGVFFGPPHAQATRQLL
                     GWPELVTVTDLASAKAAIATIVEQGEGTCGDWREAHFGRLAGILDEFLAATQADPGFS
                     PAWPARPAYVRRPPDQPQAVVIGDPLTARVADLFDAAHQTMLQALCRLWVRSGDDDAG
                     GTGDGEAA"
     gene            complement(1054..1845)
                     /locus_tag="VF995_03435"
     CDS             complement(1054..1845)
                     /locus_tag="VF995_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX9342654.1"
                     /translation="MTPLAHNRYCDEIITQTKLLRETVKGADLTAIVPTCPDWTLAEL
                     VRHIGGNLRFVETAVRTRTAVDDPQRQVPDGAGPDADDPAALDAWLADAAARFADTLR
                     AAGSDAEAQVWVFNRRTAFWARRAALDLVVHRADAAGTVGADYSVAPELAADAIDELL
                     ELFGDPEIAAASPQLAEPHGAGGSIHFHATDADAALAAEWLIELGSDGFSWRRGHERA
                     TVALRGPLTDVLLVFYRRLPPGSERVEVLGDAALLDFWLDRVALR"
     gene            1921..3591
                     /locus_tag="VF995_03440"
     CDS             1921..3591
                     /locus_tag="VF995_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019327324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9342655.1"
                     /translation="MVANGHKPVLLTVDDDPGVSRAVARDLRRRYGQDYRVVRAESGR
                     QALEALREMKLRGQVAAVLLADYRMPEMNGLEFLEQAMDLFPHARRALLTAYADTDAA
                     IQAINVVDLDHYLLKPWDPPEEKLYPVVSALIELWRATGEKPVSATQVVGHRWSAHSF
                     EVRDFLARNSVPYRWLTAEQPEGRRLLEAAGLDATSIPLVVTPDGDTLVDPSAEELAA
                     RVGLSTTPTTDFYDTVIVGGGPAGLGAAVYAASEGLRTVLVERQATGGQAGQSSRIEN
                     YLGFPDGVSGAQLTDRARRQAHKFGAEVLTARGVAAVEVRESSRVVRFTDGVEIAAHT
                     VVLATGVAYHRLDVPGADRLTGLGLYYGSAATEAPACAGEDVYVVGGANSAGQAAMFL
                     SRHARRVTLLVRADGLERSMSYYLIRQIRETPNVVVRPYTQVIGAAGDEHLEQLILCD
                     FRDGRTETVPASYLFVFIGAAPCTDWLDGVVARDPKGFLLTGPDLLAGGQRPAGWPLD
                     RDPYYLEGSVPGIFAAGDVRANSVKRVASAVGEGAMAIQLVHRYLEAS"
     gene            3588..>4294
                     /locus_tag="VF995_03445"
     CDS             3588..>4294
                     /locus_tag="VF995_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9342656.1"
                     /translation="MTAERLSPDELRELFLFEKLNGEQLAWLAKRGEVRHYPAGTVVH
                     SSGEPAVLLFVLLEGAISLSVRAGGAEIEVNRTDYRGTYSGAFLAYLDLPNAQSYRGT
                     LRTVTGCRFWALPAADFGWAVREWFPMATHLLEGFAVQGMGTMETVSTRERLVALGTV
                     TAGLTHELNNPATATARAATALRERLSALLDELVIIAHACSPSPTPTSGEGQIATLVD
                     LVRQTLHPDHRAELGPLS"
BASE COUNT          581 a         1520 c         1600 g          593 t
ORIGIN      
        1 cgccgcctca ccgtcgccag tgccgccggc gtcgtcatcg ccgctgcgga cccagaggcg
       61 gcacagcgcc tgcagcatgg tctggtgggc ggcgtcgaac aggtcggcga cccgcgcggt
      121 cagcgggtcg ccgatcacca ccgcctgggg ctggtcgggt gggcggcgca cgtacgcggg
      181 ccgcgccggc caggccggcg agaagcccgg gtcggcctgg gtcgccgcca ggaactcgtc
      241 gaggatgccg gccaggcggc cgaaatgggc ttcccgccag tcgccgcagg tgccctcgcc
      301 ctgctcgacg atggtggcga tggcggcctt cgccgaggcg aggtcggtga cggtcaccaa
      361 ctccggccag ccgagcagct ggcgggtcgc ctgcgcgtgg ggcgggccaa agaacacacc
      421 cgcctcgccg tagcgctcga cgagccggct gaagccgtgc tcgatgctgc gatagagatg
      481 cccgaccgtg gagtagtcct gcggggcggc catcatggtg gtcggggtcg cggtcagcgg
      541 ccggcattcg cacgccgcgg cgaaggcgtc ggcgtcctcg acgtcgatgt ggtcggggcg
      601 ttccaggtag aggaagtggc gcagcgccgc ctcaccgaac ggcgccagcg cgatccgcac
      661 gtcaggcggg taccaccggc agctgatcgg gaagttcggc cggtggaaat gcggtgtggc
      721 gccgaccgcg gtcagcaggt tggtggccag ggtcaggtgc agcatctcct gcacggcgat
      781 ctgggacagg gtctggcccc agcgggtcac ctgggcggcc tgctcggcgc tcaatccctc
      841 ctggcagtgg gggtcgcgtt tcagtgagta gagggcgaac aggtactcgc agagcacccc
      901 ctgttccaac tcgcaggcct gcgtgagcag atagacgagc tgctcgcggc tgtcgattgg
      961 cagtggcggc cgggtggtgg tggtcgtcac gccgggatac cgagggacgg gacgggacac
     1021 gggcacgggc aacctccttc acgcggcagc gcgtcagcgc agggcgacac gatcgagcca
     1081 gaaatccagc agggcggcgt cgcccagcac ctccacccgc tcgctgcccg gggggagccg
     1141 gcggtagaag accagcagca cgtcggtgag cgggccgcgc agcgcgaccg tggcgcgctc
     1201 gtggccgcgg cgccagctga agccgtcgct gccgagctcg atcagccact cggccgccag
     1261 cgccgcgtcg gcgtcggtcg cgtggaagtg aatgctcccg ccagcgccgt gcggctccgc
     1321 cagctgcggc gaggcggccg cgatctccgg gtcgccgaac agctccagca gctcgtcgat
     1381 ggcgtcggcg gccagctccg gcgcgacgct gtagtcggcg ccgaccgttc cggccgcgtc
     1441 ggcgcggtgg accaccaggt cgagcgcggc acggcgggcc cagaaggctg tcctgcggtt
     1501 gaagacccag acctgcgcct cggcgtcgga gccggcggcg cgcagggtgt cggcgaagcg
     1561 cgcggcggcg tcggcgagcc aggcatccag ggcggccggg tcgtcggcgt ccggtccggc
     1621 gccatcgggg acctgccgct gcgggtcgtc caccgccgtc ctggtccgga cggcggtctc
     1681 gacgaagcgg agattgccgc cgatgtggcg cacgagctcg gcgagcgtcc agtcggggca
     1741 ggtcgggacg atcgcggtca ggtcggcgcc cttcaccgtt tcccggagca gcttggtctg
     1801 cgtgatgatc tcgtcgcagt agcggttatg tgctaaaggc gtcatgtggg cacgctacac
     1861 caggggcgcg accgctcgcg ttgtcgcctg tcgctggtga tccgtaccat cgggagcgct
     1921 gtggttgcga acggacacaa gccagtcctg ctgacggttg acgacgaccc cggggtctcg
     1981 cgcgcggtcg cgcgcgacct gcggcgccgg tacgggcagg actaccgggt cgtgcgagcc
     2041 gagtcgggac gccaagccct ggaggcgctc cgggagatga agctccgcgg ccaggtcgcg
     2101 gcggtactgc tggccgacta ccgcatgcca gagatgaacg gcctggagtt cctggagcag
     2161 gccatggacc tgttccccca cgcgcgccgg gcgctgctga ccgcctacgc cgataccgac
     2221 gcggcgatcc aggcgatcaa cgtggtggac ctcgaccact acctgctgaa accgtgggat
     2281 ccgccggagg agaagctcta cccggtggtc agcgcgctga tcgagttatg gcgcgccacc
     2341 ggggagaagc cggtcagcgc cacccaggtg gtcgggcatc gctggtcggc gcactcgttc
     2401 gaggtgcggg acttcctggc gcgcaactcg gtgccctacc gctggctcac cgccgagcag
     2461 ccggagggcc ggcgcctgct cgaggccgcc ggcctcgacg ccaccagcat cccgctggtg
     2521 gtgacccccg acggcgacac cctggtcgac ccgagcgccg aggagctggc cgcccgggtg
     2581 ggcctgtcca ccacgcccac caccgacttc tacgacaccg tgatcgtcgg cggcggcccc
     2641 gccgggctcg gcgcggccgt ctacgccgcc tcggaggggc tgcggacggt cctggtcgag
     2701 cggcaggcga ccggcggcca ggccgggcag agctcccgga tcgagaacta cctcggcttc
     2761 cccgacgggg tcagcggtgc gcagctcacc gaccgggccc gccgccaggc ccacaagttc
     2821 ggcgcggagg tcctcaccgc tcgcggcgtc gccgccgtcg aggtgcgcga gtcctcccgg
     2881 gtggtgcgct tcaccgacgg ggtcgaaatc gccgcccaca cggtggtgct ggccactggg
     2941 gtcgcctacc accggctcga cgtgcccggt gccgaccggc tcaccggcct ggggctctac
     3001 tacgggtcgg ccgcgaccga ggcgcccgcc tgcgcgggcg aggacgtcta cgtcgtcggg
     3061 ggcgccaact ctgccgggca ggcggcgatg ttcctgtcca ggcacgcccg gcgggtcacg
     3121 ctgctggtgc gcgccgacgg cctggaacgg tcgatgtcct actacctgat ccgccagatc
     3181 cgcgagacgc ccaacgtcgt ggtgcggccc tacacccagg tgatcggggc ggcgggcgac
     3241 gagcacctgg agcagctcat cctctgcgac ttccgcgacg gccgcaccga gaccgtgccg
     3301 gcgagctacc tgttcgtgtt catcggcgcg gcgccctgca ccgactggct cgacggcgtg
     3361 gtcgcgcgcg accccaaggg gttcctgctg accggcccgg acctgctggc cggcggccag
     3421 cggccagcgg gctggccgct ggaccgcgac ccgtattatc tggagggcag cgtgccgggg
     3481 atcttcgcgg ccggcgacgt ccgcgccaac tcggtgaagc gggtcgcctc cgccgtcggc
     3541 gagggcgcga tggcgatcca gctcgtccac cgctacctgg aggcatcatg accgccgagc
     3601 ggctcagccc tgacgagctg cgggagctgt tcctgttcga gaagctgaac ggggagcagc
     3661 tggcctggct ggcgaagcgc ggcgaggtca ggcactaccc cgccgggacg gtggtccatt
     3721 ccagcggcga acccgcggtg ttgctgttcg tcctgctgga gggcgccatc tcgctgtcgg
     3781 tgcgggcggg cggcgccgag atcgaggtga accggaccga ctaccggggg acctactcag
     3841 gtgcgttcct cgcctacctg gacctgccca acgcgcagtc gtaccgcggc accctgcgga
     3901 cggtgaccgg ctgccgcttc tgggcgctgc ccgccgccga cttcggctgg gcggtgcgcg
     3961 agtggttccc catggcgacc cacctgctgg aggggttcgc ggtccagggg atgggcacca
     4021 tggagacggt cagcacccgc gagcggctgg tcgccctcgg cacggtcacc gccgggctca
     4081 cccacgagct gaacaacccg gcgaccgcca ccgcgcgtgc cgccaccgcg ctgcgcgagc
     4141 ggctgtcggc cctcctggac gaactggtga tcatcgccca cgcctgcagc ccgtcgccca
     4201 cgcccacgag cggcgagggt cagatcgcca ccctggtcga cctggtccgc cagaccctcc
     4261 acccggatca ccgtgccgag ctgggcccgc tctc
//
LOCUS       DASWDC010000107         7546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1361752, whole genome shotgun sequence.
ACCESSION   DASWDC010000107 DASWDC010000000
VERSION     DASWDC010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361752"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VF995_03450"
     CDS             complement(<1..162)
                     /locus_tag="VF995_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX9342657.1"
                     /translation="MDQRTGYELERQLSGGGAARIAGVDEVGRGAWAGPVMVGAVIVR
                     PGFPAPPEGL"
     gene            complement(226..870)
                     /locus_tag="VF995_03455"
     CDS             complement(226..870)
                     /locus_tag="VF995_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9342658.1"
                     /translation="MRVVIAEDAAILREGLAALLTDRGHEVCAAVADAEALRGAVASH
                     RPDVAIVDIRMPPTHTDEGLRAAIELRREHPGTGVLVFSQYIEAGYATRLLATGSAGV
                     GYLLKERVAKVAEFTDALTRVAAGGTALDPEVVTQLARASRRADDLAALTPREREVLS
                     LMAEGRSNAAIATTLVIGAGAVEKHVAGIFAKLGMPPSEDDNRRVLAVLAYLNQ"
     gene            complement(888..2327)
                     /locus_tag="VF995_03460"
     CDS             complement(888..2327)
                     /locus_tag="VF995_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016641111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEX9342659.1"
                     /translation="MASTTPMTGTPSPSSPPRRRRPGGGDAVATVAAVARAAVETPFT
                     RRAWGEFAYCLAGFPLGVAGFAGTVVLLALGAGLSLTMAGMVLGVPLLIGVLALVRGL
                     GALHRRLAAALLGERVAAPPRPRPGRGLTGRLGARLRDRASWRAVAYLLVKLPVGILG
                     LYSVALWGGGLVNMTYVLWWAGFRHHPPGTHLRPVPVLTLLPMGSFQVATFAGTFAAF
                     AVGAATVLLAPWLVRAVAAADLRLVRGLLGPGALAERVRALEETRALAVDDAAATLRR
                     LERDLHDGTQARLIALAMNLGLASEKLGDGDAGAGQPPEVARARELVASAHQTATEAI
                     AELRDLARGIHPPVLDTGLADALATLAARSAVPVRLSVEIPRRPTAAIETIAYFCAAE
                     LLANVTRHSGARHASVDAAGHDGVLRLRVSDDGRGGARLDTGTGAGSGSGSGLRGLAQ
                     RVRTVDGSLMISSPPGGPTVVSVELPLHA"
     gene            complement(2344..3126)
                     /locus_tag="VF995_03465"
     CDS             complement(2344..3126)
                     /locus_tag="VF995_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9342660.1"
                     /translation="MTTTAVPHESRGHYRFSQVARMEWIKLRSLRSTWWTLTLTVAGA
                     VAIAVAVGANTKDAAADLTNNVLAGTSLGLLLSGAVGVLAMTSEYSSGMIRATLAAIP
                     NRPLALAAKAAVVGAVTFAAGEGASFVAFLAGRAAMPATVPAPSLGQPGVLRAVMLGG
                     AGFCLIGLIGLGLGAIIRHSGAAIAVLVGGVYLAAQFVALVAHSIMPYLPVAIVANSL
                     TVVRPIVDPIRFLSPWAGLGVLSLEAAVTLAAGAWLLARRDA"
     gene            complement(3123..3845)
                     /locus_tag="VF995_03470"
     CDS             complement(3123..3845)
                     /locus_tag="VF995_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9342661.1"
                     /translation="MIEVRGISKRYGATVAVDRLSFDVRPGVVTGFLGPNGSGKSTTM
                     RMILGLDRPDAGSVRIGGRRYRELGWPLREVGGLLEARAFHPGRSARAHLAALAASNG
                     LPRSRVEEVLGIVGLAGAAQRRAGKFSLGMAQRLGIAAALLGDPGVLLLDEPVNGLDP
                     EGIRWIRNLLKSLAAQGRAVLVSSHLISEIALAADHLVVIGQGRLLADTSVAELAARS
                     TSLEEAFFQLTDGAIESQGQKS"
     gene            complement(3982..4635)
                     /locus_tag="VF995_03475"
     CDS             complement(3982..4635)
                     /locus_tag="VF995_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342662.1"
                     /translation="MLGCKRVLLSNDYYPALLRDNVALVTEPIREVVPDGIVTADGTR
                     HQVDTIIFSTGFQVTDTPVKDHIRGRGGRLLAESWTPTMHAHLGTMVCGFPNLFLLLG
                     PNTGLGHTSVVLMVESQLAQVVAVLRHLRRSGRRSGAVAIEPTVEAQRRHSAWIDARM
                     AGTVWTAGGCASWYLDATGRNSTLWPGFATGYRLRLRRFRPGDYILSAAVAAPAPPA"
     gene            complement(4932..5036)
                     /locus_tag="VF995_03480"
                     /pseudo
     CDS             complement(4932..5036)
                     /locus_tag="VF995_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
     gene            complement(5100..6854)
                     /locus_tag="VF995_03485"
     CDS             complement(5100..6854)
                     /locus_tag="VF995_03485"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9342663.1"
                     /translation="MASVWLAWRPGSHNAADVLDGRGAGRREPVAVAPRNKAPQRAGR
                     FAQAAAEEFRLLVEGVKDYAIFLLDRNGYVMTWNAGAERIKQYSAAEILGEHFSRFYP
                     PADREAGRPAMALETAAREGRFEGHGWRVRKDGSRFYAHVIITALFDEHGELHGFAKV
                     TQDATAQRETERLLREREQQLADAQELAELGGFDWDIRANRVSWSPELCRIYGLEPRA
                     FTSTLNLLARSVHPDDRSLLVGALRRAASTGSPFRLEQRIVWPDGTVRYLLIRARVVR
                     DERGRPQRVLGVCLDLTDQREGERQLAEASAQADLSRELQNGLLPDISLRDPTLVLRT
                     RYRAGLERALLGADFYDALELPDGTVATLIGDVAGHGPGEAAVGVALRAAWRALILAG
                     HGPKDVLDGLDRVLRSARRSEELFATACLLWVSPDRGSVAVTLAGHPPPLLARDGTVE
                     VVEVPSGPALGILDDDYPWEAGTLTVGDAWTLLCYTDGLVEGQRAPGSVERFGIEAVS
                     EAADKLLQGETSADLDRLLDGLLDVVVEANGEDLSDDVAMLCLTTAEREQSASGSGLG
                     PGRGEATVDIEVLGETAR"
     gene            7087..>7546
                     /locus_tag="VF995_03490"
     CDS             7087..>7546
                     /locus_tag="VF995_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9342664.1"
                     /translation="MRVEQEVRGGCVIVTLNGALDLGGVAEVRRVLWKRLADQPLAVI
                     CDLAGVCRVDPRSAGVFSSVAGHAAERWPETVLVLAGAAPEMVTALSRQGVTRLVPLY
                     GTLKEAMRHVDERPSYRYDELLLMPTAAAPGIARRFVQGLYGSWWGPEGDG"
BASE COUNT         1061 a         2730 c         2785 g          970 t
ORIGIN      
        1 cagcccttcc ggcggcgccg ggaagcccgg ccggacgatc acggcgccga ccatgaccgg
       61 tccggcccac gcgccccggc cgacctcgtc gacgccggcg atccgggccg ctcccccgcc
      121 ggacagctgg cgttcaagct cgtatccggt cctctggtcc atggcgaacc atgatgccgt
      181 tcgcgcgcgg cggccgcctt gggttcgcag cccgaactgc gcccgttact ggttgaggta
      241 ggcgaggacg gccaggacgc ggcggttgtc gtcttccgac ggcggcatgc cgagcttggc
      301 gaagatgccg gcgacgtgct tctccaccgc gccggcgccg atcaccaggg tggtggcgat
      361 cgcggcgttg gagcgcccct cggccatcag cgagaggacc tcacgctccc ttggggtgag
      421 cgcggccagg tcgtcggcgc ggcggctggc ccgcgcgagc tgggtgacca cctcggggtc
      481 cagcgcggtg ccgcccgcgg ccacccgggt gagcgcgtcg gtgaactccg ccaccttggc
      541 gacccgctcc ttgagcaggt agccgacccc cgccgagccg gtggcaagca ggcgggtggc
      601 gtatccggcc tcgatgtact gggagaagac cagcacgccc gtccccgggt gctcgcggcg
      661 cagctcgatc gccgcccgca ggccctcgtc ggtgtgggtg ggcggcatgc ggatgtcgac
      721 gatggcgacg tcggggcggt ggctggcgac cgcgccgcgc agcgcctcgg cgtcggcgac
      781 ggcggcgcac acctcgtggc cgcggtcggt gagcagcgcg gcgagcccct cccgcaggat
      841 ggcggcgtcc tcggcgatca ccacgcgcat ggccatgatc gtcgcgccta ggcgtgcagc
      901 ggcagctcga cgctgaccac cgtcgggccg cccggcgggc tggagatcat caagctgccg
      961 tccacggtgc ggacccgttg cgccagcccg cgcaggccgc tgccgctgcc gctgccggcc
     1021 ccggtgccgg tgtcgagccg ggcgccgccg cggccgtcgt cgctgacgcg caggcgcagc
     1081 accccgtcgt gcccggccgc gtcgaccgag gcgtggcgcg cgccgctgtg cctggtcacg
     1141 ttggcgagca gctcggcggc gcagaagtag gcgatcgtct cgatcgccgc ggtgggccgc
     1201 cgcgggatct cgaccgacaa cctgaccggg acggcgctgc gggccgccag cgtcgccagc
     1261 gcgtcggcca ggccggtgtc cagcaccggc gggtggatcc cgcgggcgag gtcgcggagt
     1321 tcggcgatcg cctcggtggc ggtctggtgc gcgctagcga ccagctcgcg ggcacgggcg
     1381 acctcgggcg gctggccggc gccggcgtcg ccgtcgccga gcttctcgct ggccagcccg
     1441 aggttcatcg ccagggcgat caggcgggcc tgcgtcccgt cgtgcaggtc gcgctccagg
     1501 cgccgcagcg tcgccgcggc gtcgtccacc gccagcgccc tggtctcctc cagggcccgg
     1561 acccgctcgg cgagcgcgcc cgggccgagc agcccgcgga ccagccggag gtccgctgcc
     1621 gccaccgccc ggaccagcca cggggccagg agcaccgtcg ccgcgccgac cgcgaacgcg
     1681 gcgaaggtgc ccgcgaaggt ggcgacctgg aacgacccca tcggcagcag ggtgaggacc
     1741 ggcacgggcc gcaggtgcgt gccgggcggg tggtgccgga accctgccca ccacagcacg
     1801 taggtcatgt tgaccagccc gccgccccac agggcgaccg agtagagccc gaggatcccg
     1861 accggcagct tcaccagtag gtacgccacc gcccgccagc tggcgcggtc ccgcagcctg
     1921 gcgcccaggc ggccggtcag gccacggccg ggccggggcc gtggcggcgc ggcgacccgc
     1981 tcgcccagca acgccgcggc caggcgccgg tgcagcgcgc cgagccctcg gaccagggcg
     2041 agcaccccga tgagcagcgg caccccgagg accatgccgg ccatggtgag cgacaggccg
     2101 gcgccgagcg cgagcagcac gacagtgcca gcgaagccgg cgacgccgag cgggaacccc
     2161 gccaggcagt aggcgaactc gccccaggcc cggcgggtga acggcgtctc gaccgccgcg
     2221 cgagcgaccg cggcgacggt cgccaccgcg tccccgccgc cgggtcggcg acgccgcggc
     2281 ggggacgagg gcgagggcgt gccggtcatc ggtgtggtgc tggccatgcg cccattgtcc
     2341 tgctcacgcg tcgcgtctcg ccagcagcca cgcgccggcg gcgagcgtca cggcggcctc
     2401 gaggctgagc accccgaggc cggcccaggg cgacaggaag cggatcgggt cgacgatcgg
     2461 ccgcaccacg gtcagcgagt tggcgacgat cgcgaccggc agatacggca tgatcgaatg
     2521 cgccaccaag gcgacaaact gcgccgcgag gtacacaccg ccaacgagca cggcgatcgc
     2581 ggcgccgctg tggcggatga tcgcgccaag gccaagcccg atcagcccga tcaggcagaa
     2641 ccccgccccg cccagcatca ccgcccgcag cacgccgggc tggccaagcg acggggctgg
     2701 caccgtggcc ggcatggcgg cccgcccagc gaggaaggcg acgaagctgg cgccctcccc
     2761 ggctgcgaag gtgacggcgc cgaccaccgc cgccttggcg gccagcgcca gcggccggtt
     2821 ggggatcgcg gccagggtcg cgcggatcat cccggaggag tattcgctgg tcatcgccag
     2881 cacccccacg gcgccgctca gcagcaggcc gagggaggtc ccggcgagga cgttgttggt
     2941 gaggtcggcg gctgcgtcct tggtgttggc gccgacggcg accgcgatcg cgaccgcccc
     3001 ggcgacggtg agcgtcagcg tccaccaggt ggagcgcagg ctccgcagct tgatccactc
     3061 catccgcgcc acctgggaga accggtagtg gccgcgggac tcgtgcggga cggcggtcgt
     3121 cgtcatgact tctgcccctg agactcgatg gcaccgtcgg tcaattggaa aaacgcctcc
     3181 tccagcgagg tcgaccgggc cgccagctcc gccaccgagg tgtcagcgag cagccgcccc
     3241 tggccgatca ccaccagatg gtcggcggcg agcgcgatct cgctgatcag gtggctggag
     3301 acgagcacgg cgcggccctg cgcggccagc gacttcagca ggtttcggat ccagcggatc
     3361 ccctcggggt cgagcccgtt gaccggctcg tccagcagca gcaccccggg gtcgccgagc
     3421 agcgccgcgg cgatgccgag ccgctgcgcc atgcccagcg agaacttccc cgcccgccgc
     3481 tgcgcggccc cggccaggcc gacgatgccg aggacctcct cgacccggga gcgtggcagg
     3541 ccgttgctgg cagccagcgc cgccagatgg gcacgggcgc tgcggccggg gtggaacgcc
     3601 cgcgcctcca gcaagccccc gacctcccgg agcggccagc caagttcgcg gtagcggcgc
     3661 ccgccgatcc gcacgctgcc ggcgtcgggc ctgtccaggc cgaggatcat ccgcatggtg
     3721 gtggatttcc ccgagccgtt cgggccgaga aagccggtca cgacgcccgg ccgcacgtcg
     3781 aagctgagcc ggtcgaccgc gacggtggcg ccgtagcgtt tgctgatccc tcgaacctcg
     3841 atcatcgcaa cctcctctgg cacgcggtcg cgcgccgtgg agatcacgct atgcgggagt
     3901 ccgccgcgcc ggaatccgac caccccccga gttcaggcgg gggatttccc ccctgcggcc
     3961 ggggacacgt tgtccgggtg atcaggcagg cggcgcggga gccgcgacgg cggcggagag
     4021 gatgtagtcg cctgggcgga agcggcgcag ccgcaggcgg tagccggtgg cgaagccggg
     4081 ccacagggtg gagttgcggc cggtggcatc cagataccag ctggcgcagc cgccggcggt
     4141 ccagacggtc ccggccatcc gcgcgtcgat ccaggcgctg tggcgccgct gcgcctcgac
     4201 ggtcggctcg atcgcgaccg cgccgctgcg gcgtccgctg cgacgcaggt gccgcaggac
     4261 ggcgacgacc tgggcgagct ggctctcgac catcagcaca accgaggtgt ggccaagccc
     4321 ggtgttcggg ccgagcagca ggaacaggtt cgggaaccca cagaccatgg tgccgaggtg
     4381 ggcgtgcatc gtcggcgtcc acgactcggc cagcagccgc ccgccgcggc cgcggatgtg
     4441 gtccttgacg ggcgtgtcgg tgacctggaa gccggtgctg aagatgatcg tgtcgacctg
     4501 gtgccgggtc ccgtcggcgg tgacgatccc gtcggggacg acctcacgga tcggttcggt
     4561 gaccaacgcg acgttgtcgc gcagcagcgc cgggtagtag tcgttggaga gcaggacccg
     4621 cttgcagccg agcacgtagt ccggggtgag ggcggcccgc agcccagggt cggggacctg
     4681 gcggcgcagg tgccgcgcgg cgagccgctg gaccaggcgg tgaaggctcc ccgcgaggtc
     4741 tggacctgcc agcgctgcgc ggcgtcgtcc caggccagct cctggacggc atggccgagc
     4801 cgcaggtgcg ggccgatgcc gaaccgctcc acgcagccgc gcaggtacgc ccagatctcc
     4861 cgctgccccg agaaggtgtc ggaccagtcc gggttcggcg cgaaggagaa gcagtacagg
     4921 tgcgacggca cgtcgcaggc gcagccgggg taggtgttgt cgcgccaggt gccgccaacg
     4981 tcgtcggcct gctcgaagac gaggaaatcg tggaagcctt cccgccgcag gcggatcgcc
     5041 gcgcggcagg cctcacatta ctagttagta acctcccgcg agggaaaccg caaggccgct
     5101 cagcgagctg tttcgccgag gacctcgatg tccacggttg cctcgccacg tccggggccc
     5161 agtccggagc cggaggcgga ctgctcgcgc tcggcggtcg tgaggcacag catcgcgacg
     5221 tcatcggaca ggtcttcgcc attggcctcg acaaccacgt cgagcagccc gtccagcagg
     5281 cggtccagat ccgcgctcgt ctcaccctgc agcagcttgt cggcggcctc gctgaccgcc
     5341 tcgatcccga accgctccac cgatccgggc gcccgctgcc cctcgaccag cccgtcggtg
     5401 tagcacagca gcgtccaagc gtccccgacc gtgagcgtgc cggcctccca gggatagtcg
     5461 tcgtcgagga tgccgagcgc cgggccgctg ggcacctcga cgacctcgac cgttccgtcg
     5521 cgggcgagca gcggcggggg atggccggcc aaggtgaccg cgacgctgcc tcggtcgggg
     5581 ctgacccaca gcaagcaggc ggtggcgaac agctcctcgg agcgccgcgc cgaccgcagc
     5641 acccggtcca gcccgtcgag cacgtccttg ggtccgtgcc cggccaggat cagcgcccgc
     5701 cacgcggccc gcagcgcgac cccgaccgcg gcctcgccgg gaccgtgccc ggcgacgtcg
     5761 ccgatcaggg tcgcgaccgt gccgtcgggc agttccagcg cgtcatagaa gtccgcgccg
     5821 agcagggcgc gttcgaggcc ggcccggtag cgggtgcgca gcaccagcgt cgggtcgcgc
     5881 agcgagatgt ccggcaggag cccgttctgc agctcgcgcg acaggtcggc ctgggcggat
     5941 gcctcggcga gctgccgctc gccctcacgc tggtcggtca ggtccaggca gacgccgagg
     6001 actcgctgcg gccggccgcg ctcgtcacgc acgacccggg ccctgatcag caggtagcgg
     6061 accgtgccgt cgggccacac gatgcgctgc tccagccgga acgggctccc ggtgctggcc
     6121 gcccggcgga gcgcgccgac cagcagcgac cgatcgtcgg gatggacgct gcgggcaagc
     6181 aggttgagcg tcgaggtgaa cgcccggggc tccaggccat agatccggca cagctccggg
     6241 ctccaggaca cccggttggc gcggatgtcc cagtcgaagc cgcccagctc ggcgagttcc
     6301 tgcgcgtcgg cgagctgctg ctcacgttcc cgcagcaggc gttcggtctc gcgctgcgcg
     6361 gtcgcgtcct gggtgacttt ggcgaatccg tgcagctcgc catgttcgtc gaagagcgcg
     6421 gtgatgatca cgtgcgcgta gaagcggctc ccgtccttgc ggacgcgcca gccgtgcccc
     6481 tcgaagcggc cttcccgcgc cgcggtctcc agcgccatgg cgggccgccc ggcctcccgg
     6541 tccgcgggtg gataaaagcg cgagaaatgc tcgccgagga tttcggcagc gctgtactgc
     6601 ttgatccgct cggcgcccgc gttccaggtc atcacataac cgttgcggtc aagcaggaag
     6661 atggcgtagt ccttcacgcc ttcgaccagg agccggaatt cctctgccgc tgcttgagcg
     6721 aaccgtccgg cgcgttgtgg tgccttgttc cgcggtgcca ccgctaccgg ctcacgcctt
     6781 cccgcccctc gtccatccag cacgtcggca gcattgtggc tgcctggtcg ccaggcgagc
     6841 cagactgacg ccatcagcgc tcagctgtca accgtggacc caaatcaacc actacctata
     6901 tgagcaggct gaagcctctc tacacgcagc ttcagtaatt caggcggaaa taggtacagt
     6961 ggcgggctgg accggctgga tgcgggttga ccggtgccgg tggcgggtag gccactccgg
     7021 gtaccgagct gtcctggcac cgaacaaaga cggcaaggcc gagacagcgc tcagacaggc
     7081 ggggcgatgc gggtcgagca ggaggtgcgg gggggttgcg tgatcgtgac cctgaacggt
     7141 gcgctggatc ttggcggcgt cgccgaagtg cgccgtgtgc tgtggaaacg cctcgccgac
     7201 cagccgctgg cggtgatctg cgacctggcc ggcgtgtgcc gggtcgaccc ccgctccgcc
     7261 ggggtgttct cgtcggtcgc aggccatgcg gcggaacgct ggcccgagac cgtcctggtg
     7321 ctggccggcg cggctcccga gatggtaacg gcgctgtccc gccagggggt gaccaggctg
     7381 gtcccgctgt acggcaccct caaggaggcc atgcggcacg tcgacgagcg gccctcctac
     7441 cggtacgacg agctgctgct gatgccgacc gccgccgccc ccgggatcgc caggcggttc
     7501 gtgcaagggt tatacggctc ttggtggggg ccggaggggg acgggg
//
LOCUS       DASWDC010000108         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1361811, whole genome shotgun sequence.
ACCESSION   DASWDC010000108 DASWDC010000000
VERSION     DASWDC010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361811"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..781
                     /locus_tag="VF995_03495"
     CDS             2..781
                     /locus_tag="VF995_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018959717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9342665.1"
                     /translation="MASTIRVLLVDDQALVRTGFRMILAAAPDLEVVGEAGDGAEAVE
                     LAQATRPDVVLMDVRMPGVDGIEATRRIRQIASSTAAAASVAPLGDPGAKAPAERPAP
                     RVLILTTFDLDEYVFDGLRAGASGFLLKDSLAPDLLAAIRVVASGDAVVAPAITRRLL
                     ERFIHNTPAPDRSDTRRLEVLTPREREVLELIGRGCSNAEIAERLYLSEGTVKTHVSR
                     VLAKLGLRDRVHAVILAYETGLIRAGDAGAGDGDGDARDQG"
     gene            complement(772..1596)
                     /gene="ppk2"
                     /locus_tag="VF995_03500"
     CDS             complement(772..1596)
                     /gene="ppk2"
                     /locus_tag="VF995_03500"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX9342666.1"
                     /translation="MTTDKVTHPAKAKTPAKMKRKPYERELERLQIELVELQDWIKHE
                     GLRVVVLFEGRDAAGKGGVIKRITERLNPRICRVVALGTPTERDKTQWYFQRYAAELP
                     AAGEMVLFDRSWYNRAGVERVMGFCTEAEVEEFFRSCPQFEDMLIRSGIILIKYWFSV
                     SDEEQERRFQERANDPTKRWKLSPMDLKSREHWLDYSRAKDEMFAYTDTKTSPWWVVN
                     ADVKRTARLNCISHLLSRIPYEDVTPKPIKLPPRQEHGKYARPPLSDQTFVPERYP"
     gene            1705..2658
                     /locus_tag="VF995_03505"
     CDS             1705..2658
                     /locus_tag="VF995_03505"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9342667.1"
                     /translation="MRPSYDELLSGAAGTRVPGSSWGLWGDGDPEVGTLNDISAADVV
                     AAATLVARGAVFSLNWSLALPSPALFSRGPIEHVLRDDGFGMDDHLNRFYPQASSQWD
                     ALNHFAHPEHGFYGGRTSDRLKGPQAENSIANLARRGIAGRFVLADVGRWRDRQGRPI
                     DQEGGEVIPVADVAATLAAQGSAVRPGDILLLRYGWTTWYEGLDQAGREALAALDDWA
                     FKAAGLEHGEDAVRWLWDSGVAAAVADNPSFEAFPFDPRGDNLHSSLIALLGMPLGEL
                     WQLDELAADCTADGRYTGLLASAPLNMPGCVGSPANALALK"
BASE COUNT          386 a          984 c          994 g          442 t
ORIGIN      
        1 catggcgagc accatccggg tcttgctggt cgacgaccag gcgctggtcc gcaccgggtt
       61 ccgcatgatc ctggcggcgg cgccggacct ggaggtggtc ggcgaggccg gcgacggcgc
      121 cgaggcggtc gagttggcgc aggccacccg gcccgacgtg gtgctgatgg acgtccgcat
      181 gcccggagtc gacgggatcg aggcgacccg ccggatccgg cagatcgcca gctcgaccgc
      241 ggctgcggct tcggtggcgc cgcttggaga tccaggtgcg aaggccccgg cggagcgtcc
      301 ggcgccccgc gtgctcatcc tcaccacctt cgacctcgac gagtacgtct tcgacgggct
      361 gcgcgcgggc gccagcggct tccttctcaa agactccctc gcccctgacc tgctcgccgc
      421 gatccgggtg gtcgcctccg gcgacgccgt ggtcgctccg gccatcaccc gccgcctgct
      481 cgaacgcttc atccacaaca cgccagcgcc cgaccggtcg gacacccgca ggctggaggt
      541 gcttaccccc cgtgaacgcg aggtgctcga actgatcggc cgcggctgct ccaacgccga
      601 gatcgccgag cgcctgtacc tcagcgaggg caccgtgaag acccacgtca gccgggtgct
      661 cgccaagctc ggcctgcgcg accgggtcca tgccgtgatc ctcgcctacg agactggcct
      721 gatccgtgcc ggggatgccg gggccgggga tggggatggg gatgcgcggg atcagggata
      781 gcgttcgggg acgaaggtct ggtcgctgag cgggggccgc gcgtacttgc cgtgctcctg
      841 ccggggcggc agcttgatcg gcttgggggt gacgtcctcg tacgggatcc tgctcagcag
      901 gtggctgatg cagttgagcc gggcggttcg cttcacgtcg gcgttcacca cccaccaggg
      961 cgaggtcttg gtgtcggtgt aggcgaacat ctcgtccttg gcacgggagt agtcgagcca
     1021 gtgctcgcgg gacttcaggt ccatcgggct gagcttccag cgcttggtcg ggtcgttggc
     1081 gcgctcctgg aagcggcgct cctgttcctc gtcgctgacc gagaaccagt acttgatgag
     1141 gatgatgccc gagcggatca gcatgtcctc gaactgcggg caggagcgga agaactcctc
     1201 cacctccgcc tcggtgcaga accccatcac ccgctcgacc ccggcgcggt tgtaccagct
     1261 gcggtcgaac agcaccatct cgccggcggc gggaagctcg gcggcgtagc gctggaagta
     1321 ccactgggtc ttgtcccgct cggtcggggt gccgagcgcc accacccggc agatccgggg
     1381 attcagccgc tcggtgatcc gcttgatgac cccgcccttg ccggcggcgt cgcggccctc
     1441 gaacagcacg accacccgga ggccctcgtg cttgatccag tcctgcagct cgaccagctc
     1501 gatctggagc cgctcaagct cgcgctcgta gggcttgcgc ttcatcttgg ccggggtctt
     1561 ggccttggcg ggatgggtca ccttgtcggt tgtcatggtg gctccatcgt agccagccgc
     1621 gcgacccagg gttttgaaat tttactcact gaaggtgaaa tgtttcgatg cgggaagggc
     1681 tggctcgaca tggaggtgca gcgaatgcgt ccaagctacg acgaactgct cagcggcgcc
     1741 gccgggaccc gggtgcccgg gtcgtcgtgg ggcctgtggg gcgacggcga cccggaggtc
     1801 ggcaccctca acgacatcag cgccgccgac gtggtcgccg ccgccacgct ggtcgcgcgc
     1861 ggcgcggtgt tctcgctgaa ctggagcctc gcgctgccgt cgcccgcgct gttctcccgg
     1921 ggcccgatcg agcacgtgct gcgcgacgac ggcttcggca tggacgacca cctcaaccgg
     1981 ttctacccgc aggcctccag ccagtgggac gccctcaacc acttcgccca ccccgagcac
     2041 ggcttctacg gcggccggac ctccgacagg ctgaagggcc cgcaggccga gaacagcatc
     2101 gccaacctgg cccggcgggg gatcgccggc cgcttcgtgc tcgccgacgt gggccgatgg
     2161 cgggatcgtc agggccgccc gatcgatcag gagggcggtg aggtgatccc ggtcgccgac
     2221 gtcgccgcca ctctggccgc acagggcagc gccgtgcggc caggcgacat cctgctgctc
     2281 cgctacggct ggaccacctg gtatgagggc cttgaccagg ccgggcggga ggcgctggcc
     2341 gccctggacg actgggcgtt caaggcggcc ggcctggagc acggcgagga cgccgtccgc
     2401 tggctgtggg actccggcgt ggccgcggcg gtcgccgaca acccctcgtt tgaggcgttc
     2461 ccgttcgacc cgcgcggcga caacctgcac agcagcctga tcgccctgct cggcatgccc
     2521 ctcggcgagc tgtggcagct cgacgagctg gccgccgact gcaccgccga cggccgctac
     2581 accgggctgc tggcgtccgc gccgctgaac atgcccggct gcgtcggctc gcccgccaac
     2641 gccctggccc tcaagtgaac cggagggagc tctgatgcca gcagcggagc agggccgggt
     2701 cgccggcaag gtggtgatcg tcaccggcgc cgctcagggc cagggcgccg ccgaaagcct
     2761 ggcgctggcc cttgagggcg ccatcgtggt cgcggccgac ctgcgc
//
LOCUS       DASWDC010000109         4925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1100342, whole genome shotgun sequence.
ACCESSION   DASWDC010000109 DASWDC010000000
VERSION     DASWDC010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100342"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VF995_03510"
     CDS             complement(<1..705)
                     /locus_tag="VF995_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX9342668.1"
                     /translation="MSTQTQPQDQPQRESDRADLRWRGVAAEESDKLPSGVSTLLRAR
                     SRRLLGSLLRPHRRALILAVVVILIENAAAMAAPYLVKVGIDRGIPPLATGGRSGPLL
                     LIVALLLAAALLQAVTLRIFLILTGRLGQQLLLDLRTRVFDHFQRLSLSFHERYTSGR
                     VISRLTSDMDAIAEVLDGGLDEMAAAVLSIASITVIILLLDPPLGLVTLGTFPLLLWL
                     SAWFRRNSAAAYRRTRE"
     gene            complement(702..2414)
                     /locus_tag="VF995_03515"
     CDS             complement(702..2414)
                     /locus_tag="VF995_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342669.1"
                     /translation="MLLMLAMTMLGVGAGIVIPLVTKAIIDGPVAGGQRGKLLPLALL
                     AFGLGAVEAVFTMIRRWVQASAALGIETDMRSDLYRHLQALPVSFHDRWQSGQLLSRA
                     ISDLSVIRRFIGFGLIFLVVNFLTYITVIALLLHLDTALGLVVVAWTLPIALVSRSFA
                     RQYFTVSRRVQDQQGDVATQVEEAAVGIRVVKAFGRRRLVGERFGAAARTLYGSSMDS
                     VRLRARFWAMLDVIPNLGLGVALLLGAAAVAHQRLTVGGLVAFLALQLQLVWPIDALG
                     WLLAVGQEAATAAERVYEVLDAPLEIADRPGARPLAPRSVQGRLRFERVGFRYPGTRH
                     PVLHDVRLQIEPGETVALVGATGSGKTTLVSLVPRLYDVTEGRITLDGHDLRDLELTS
                     LRRIVAVGFEEPILFSASVRENLLLGDPDASDQRIAEALATAQAEFVNDLPWGLETRI
                     GEQGLSLSGGQRQRLALARAVLAHPQVLVLDDPLSALDVHTEALVEEALARVLAGTTG
                     LIVVHRPSTIALADRVALLEDGAITAVGTHAELLAAVPSYRAILSQDADLAASDGQAE
                     AAAS"
     gene            2693..4171
                     /locus_tag="VF995_03520"
     CDS             2693..4171
                     /locus_tag="VF995_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX9342670.1"
                     /translation="MARPAPSSELAREAIGLREVLFQSVTHMAPAAAVAFSIPFGAPF
                     GAGALPLAVVLALVACLLVAVSIGQLAKHLPSAGGFYTYTARGLHPSVGFLVAFGYAL
                     AEPLVAPLLYLILGNVVASTLHTEFGWSFGLWWRIAAVAGALVVFLLGYFGIRISAAT
                     GTILGAFEIAVFAALALWLIGKAGSHNTLSVFSTSHATVPGFKGASGVVAGSIYSILA
                     FIGFEAAAPMAEEARDPRRTIKLAVFWSALGIGLFYVLTTYAATVFFGPDKMAGFAAS
                     GGGNPWDGLARTVWGVGWVVVFLAIVNSAVANSNAGSNATTRTWFAMGRIRLLPAVFA
                     RTHPRWRSPYVAVIAQLVVGLVVSLWLGQKFGPLTAFGLIATIDTTVIILIYMLVNLS
                     CLVYYLRERRGEFNPLLHAVIPVVGILAFVPAFFTALGIGKSVFKFVSVLPYPLNRSG
                     LVVGIWFVLGILYLAWLLVRRPERVRETGRVFIDAPARETPV"
     gene            4273..>4925
                     /locus_tag="VF995_03525"
     CDS             4273..>4925
                     /locus_tag="VF995_03525"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9342671.1"
                     /translation="MLEQEQQPRRQQRRVAGRYALGAELGRGGMGTVWRATDELLGRE
                     VAVKEIALPETVGGPGQDAASIRARVLREARAAARLNHPGIVTVHDVLDALDRLFIIM
                     ELVEAPSLAAVVAKEGPLPPARVARIGLGLVDALEAAHAAGIVHRDVKPGNVLLLPGD
                     RVKLADFGIAAVQGDPQLTASKVAMGSPAFMAPSRRAAARSALRPTGGRSARLCISR"
BASE COUNT          673 a         1625 c         1907 g          720 t
ORIGIN      
        1 ctcgcgggtg cggcggtagg cggcggcgga gttgcgccgg aaccaggccg acagccacag
       61 cagcagcggg aaggtgccga gggtgaccag gccgaggggc gggtcgagca gcaggatgat
      121 gacggtgatg ctggcgatcg acaggaccgc cgcggccatc tcgtcgaggc cgccgtcgag
      181 gacctcggcg atggcgtcca tgtcggaggt gagccgcgag atcacccggc cggaggtgta
      241 gcgctcgtgg aaggagaggc tgaggcgctg gaagtggtcg aagacgcggg tgcgcaggtc
      301 gagcaggagc tgctggccga gccggccggt gaggatcagg aagatccgca gggtgacggc
      361 ctggagcagc gccgcggcga gcagcagggc gacgatcagg agcagcggcc cgctgcgccc
      421 gccggtggcg agcggcggga tgccccggtc gatgccgacc ttgaccaggt agggcgcggc
      481 catggcggcg gcgttctcga tcaggatcac cacgacggcc aggatcagcg cgcggcggtg
      541 ggggcgcagc agcgagccga gcagccggcg gctgcgggcg cgcaggaggg tggagacgcc
      601 gctggggagc ttgtcgctct cctcggcggc gacgccgcgc cagcgcaggt cggcccggtc
      661 gctctcgcgc tggggttggt cttggggctg ggtctgggtg ctcatgaggc ggccgcctcg
      721 gcttggccgt cggaggcggc gaggtcggcg tcctgggaga ggatggcgcg gtaggagggg
      781 acggcggcga gcagctcggc gtgggtgccg acggcggtga tggcgccgtc ctccagcagc
      841 gcgacccggt cggcgagggc gatggtggag gggcggtgca ccacgatcag gccggtggtg
      901 ccggccagga cgcgggcgag cgcctcctcg accagcgcct cggtgtggac gtcgagggcg
      961 gagagggggt cgtcgagcac cagcacctga ggatgggcga ggaccgcgcg ggccagggcg
     1021 aggcgctggc gctgcccgcc ggagaggctg aggccctgct cgccgatgcg ggtctccagc
     1081 ccccatggga ggtcgttgac gaactcggcc tgggcggtgg cgagcgcctc ggcgatccgc
     1141 tggtcgctgg cgtcggggtc gccgagcagg aggttctcgc gcacgctggc ggagaacagg
     1201 atcggctcct cgaacccgac cgcgacgatc cggcgcagcg aggtcagctc caggtcgcgc
     1261 aggtcgtggc cgtcgagggt gatgcggccc tcggtgacgt cgtagaggcg cggcaccagc
     1321 gacaccaggg tggtcttgcc ggagccggtg gcgccgacca gggcgacggt ctcgccgggt
     1381 tcgatctgga ggcggacgtc gtggaggacg gggtggcggg tgccggggta gcggaaaccg
     1441 acccgctcga aacggaggcg gccctgcacg ctcctcggcg cgagcggccg ggcgccgggc
     1501 cggtcggcga tctccagggg cgcgtcgagg acctcgtaga cccgttcggc ggcggtggcg
     1561 gcctcctggc cgacggcgag cagccagccg agcgcgtcga tcggccagac gagctggagc
     1621 tggagcgcga ggaaggcgac caggccgccg acggtgaggc gctggtgggc gacggcggcg
     1681 gcgccgagca gcagggccac gcccaggccg aggttgggga tgacgtcgag catcgcccag
     1741 aagcgggcgc gcagccgcac gctgtccatg gaggagccgt acagcgtgcg ggcggcggcg
     1801 ccgaagcgct ccccgaccag ccggcggcgg ccgaacgcct tgaccacgcg gatcccgacg
     1861 gccgcctcct ccacctgggt ggcgacgtcg ccctgctggt cctggacgcg gcgggagacg
     1921 gtgaagtact ggcgcgcgaa gctgcgcgag accagcgcga tcggcagcgt ccaggcgacc
     1981 accaccagcc cgagggcggt gtcgaggtgg agcagcagcg cgatcaccgt gatgtaggtg
     2041 aggaagttga ccaccaggaa gatcaacccg aagccgatga agcgccggat gacgctgagg
     2101 tctgagatcg cccgggacag cagctggccg gactgccagc ggtcgtggaa ggagaccggc
     2161 agcgcctgga ggtgccggta gaggtccgac cgcatgtcgg tctcgatgcc gagggcggcg
     2221 ctggcctgga cccagcggcg gatcatggtg aacaccgcct cgaccgcgcc caggccgaag
     2281 gccagcagcg ccagcggaag cagcttgccg cgctgcccgc cggcgacggg gccgtcgatg
     2341 atcgccttgg tgaccagtgg gattacgatc ccggcgccga cgccgagcat ggtcatcgcc
     2401 agcatcagga gcatcttggc gagatgcgga cggacgtacg accggacccg ccacagtgcc
     2461 tgcagccttc tcgatccgct cctggatccg cctctggatg gtcccacgct tcgaccatac
     2521 cggagcgagc acaccaaagc acccgaatta cgggcttcgc ggcttcactg gcgcggcttc
     2581 tgccctgtgg cgcgctcctg atggttgacc gacagttgac acgcgtccgt cccggcgttt
     2641 ctgtacttgt aatgcttgat gctgcttgat ctcttcagcg ggaggtggcg cagtggcacg
     2701 acccgcccca tcgagcgaac tggctcgcga ggcgatcggc ctgcgagagg tcctgttcca
     2761 gtccgtcacg cacatggcgc cggccgccgc cgtcgccttc tcgatcccct tcggggcgcc
     2821 gttcggcgcg ggcgcgctgc cgctggcggt ggtgctggcg ctggtcgcct gcctgctggt
     2881 ggcggtctcg atcgggcagc tcgccaagca cctgccgtcg gcgggcggct tctacaccta
     2941 caccgcgcgg ggcctgcacc cgtcggtcgg gttcctggtg gcgttcgggt acgcgctggc
     3001 cgagccgctg gtggcgccac tgctgtacct aatcctcggc aacgtggtgg cgagcacgct
     3061 gcacaccgag ttcggctggt cgttcgggct gtggtggcgg atcgccgcgg tggcgggcgc
     3121 gctggtggtg ttcctgctcg gctatttcgg gatccggatc agcgccgcga ccggcacgat
     3181 cctcggcgcc ttcgagatcg cggtgttcgc cgcgctggcg ctgtggctga tcggcaaagc
     3241 cggcagccac aacaccctgt cggtgttcag caccagccac gccaccgtcc caggcttcaa
     3301 gggcgcctcc ggggtggtgg ccgggtcgat ctacagcatc ctggcgttca tcggcttcga
     3361 agccgccgcg cccatggccg aggaggccag ggacccgcgg cgcaccatca agctggcggt
     3421 gttctggtcg gcgctcggga tcgggctgtt ctacgtgctg accacgtatg cggccacggt
     3481 gttcttcggc ccggacaaga tggccggctt cgcggcgtcg ggcggcggca acccgtggga
     3541 cgggctcgcc cggacggtct ggggtgtcgg ctgggtggtg gtgttcctgg cgatcgtgaa
     3601 ctcggcggtg gccaactcca acgccggctc caacgcgacc acccggacct ggttcgcgat
     3661 ggggcggatc cgcctgctgc ccgccgtctt cgcccgcacc catccgcgct ggcgctcgcc
     3721 gtatgtggcg gtgatcgcac agctggtggt cgggctggtg gtgtcgctgt ggctcgggca
     3781 gaagttcggg ccgctgaccg ccttcgggct gatcgccacc atcgacacca ccgtcatcat
     3841 cctgatctac atgctggtga acctctcctg cctggtgtac tacctgcgcg agcggcgcgg
     3901 cgagttcaac ccgctgctgc acgcggtgat cccggtcgtc ggcatcctcg cgttcgtccc
     3961 ggcgttcttc accgcgctcg gcatcggcaa gtcggtgttc aagttcgtca gcgtgctccc
     4021 ctacccgctg aaccgcagcg gcctggtggt ggggatctgg ttcgtgctcg ggatcctcta
     4081 cctggcgtgg ctgctggtca ggcgcccgga gcgcgtccgc gagaccgggc gggtgttcat
     4141 cgacgcgcca gcgcgggaga cgccagtgtg agcgagctgc aagcccagca cgcttgagcc
     4201 gttcggcggc cgttcaccgg ttgttaccag agtgctgctc gtgtcgagat atgcgtggtt
     4261 tacgctcagc ccatgcttga acaggagcag caaccgcggc gccagcagcg gcgcgtcgcc
     4321 ggccgctacg ccctcggggc cgaactgggc cgcggtggca tggggaccgt gtggcgcgcc
     4381 accgatgagc tgctcggccg cgaggtcgcc gtcaaggaga tcgcgctgcc cgaaaccgtc
     4441 ggcggccccg gccaagacgc cgccagcatc cgggcgcggg tgctgcggga ggcccgcgcg
     4501 gccgcacggc tcaaccaccc cgggatcgtc accgtccacg acgtgctgga cgcgctcgac
     4561 cgcctgttca tcatcatgga gctggtcgag gcgccgagcc tggcggcggt ggtcgccaag
     4621 gagggcccgc tgccgcccgc acgcgtggcc aggatcggcc tggggctggt ggacgcgctc
     4681 gaagcggccc acgccgccgg gatcgtgcac cgcgacgtca agcccggcaa cgtcctgctg
     4741 ctgcccggcg accgggtcaa gctggccgac ttcgggatcg cggcggtcca gggcgacccg
     4801 cagctcaccg cctcgaaagt ggcgatgggc tcccccgcct tcatggcccc gagcaggcga
     4861 gcggccgccc ggtcggccct ccggccgact ggtgggcgct cggcgcgact ctgtatttcg
     4921 cggtg
//
LOCUS       DASWDC010000110         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_277773, whole genome shotgun sequence.
ACCESSION   DASWDC010000110 DASWDC010000000
VERSION     DASWDC010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277773"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VF995_03530"
     CDS             <1..629
                     /locus_tag="VF995_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342672.1"
                     /translation="DWREAHFGRLAGILDEFLAATQADPAFSPAWPARPGYVRRPPDQ
                     PQAVVIGDPLTARVADLFDAAHQTMLQALCRLWVRSGDDDAGGTGDGEAATLAGIAIE
                     LMASVLRPLGSALVTLPLGPDNPGELAGPAFFMVHPSQFILPHREAAWKVIAQRLGEI
                     ADACAELRHESGLATIIAGQIAQLEPTVRGLAAQLSRSGETAERTSGA"
     gene            complement(626..1576)
                     /locus_tag="VF995_03535"
     CDS             complement(626..1576)
                     /locus_tag="VF995_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX9342673.1"
                     /translation="MLVLAAGIGLAASPAAQAAYPGHDGKVAYVITSPSGTSHDLWTV
                     NTDGTGNRRLTSDGTSWHPRWSPDGKQIAFERSKDIYIMTSTGTLVRRVTTTGRAHQP
                     TWSPNGKRLAFVNVLSNGHGDIFTVAATGGAITRITHDAATTCGDDRPTWSPLGGLIV
                     YHQLINTGSGCTHNRILLVTLSSGAERVLIEDGGVGPNGPVTNPDFMPDGLHVLFMAK
                     CFDVGDCVSVQSYNPVVVDLNGANPRFLDHSDATEGDYRSFETAGAPDGQRVVIATLS
                     WPGDPPVDSFLFDPVARTTIVSVDQASVGTIAQPDWQPLP"
     gene            2058..2507
                     /locus_tag="VF995_03540"
     CDS             2058..2507
                     /locus_tag="VF995_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342674.1"
                     /translation="MTEISHRDQVLGSAFRVLVMLEALSFLVFAVLHLGVRIPLGFAV
                     ISTDTIDDAVIAEGLSGAFLALSAAALFTRRRWALRATVFAQAFSLAAVLVGIAAIAA
                     GLGPSSLENDVYHRVMAPVLALSLVWLATPSGRGALQLSGEDATLRR"
     gene            2504..>2945
                     /locus_tag="VF995_03545"
     CDS             2504..>2945
                     /locus_tag="VF995_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020380366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9342675.1"
                     /translation="MTASTWHQFATEAPELATAVRARLEATKHHVLATLRRDGSPRVS
                     GTEVTFFGADLMVGSMWGAVKARDLQRDGRFALHANPGDGSMEGGDAKLSGRAVEITD
                     PNELRAFTKAEEPPEPYHLFRLLLDQAVLTSVANDRLVIASWRPG"
BASE COUNT          403 a         1028 c         1058 g          456 t
ORIGIN      
        1 gcgactggcg ggaagcccat ttcggccgcc tggccggcat cctcgacgag ttcctggcgg
       61 cgacccaggc cgacccggct ttctcgccgg cctggccggc gcggcccggg tacgtgcgcc
      121 gcccacccga ccagccccag gcggtggtga tcggcgaccc gctgaccgcg cgcgtcgccg
      181 acctgttcga cgccgctcac cagaccatgc tgcaggcgct gtgccgcctc tgggtccgca
      241 gcggcgatga cgacgccggc ggcactggcg acggtgaggc ggcgaccctg gcgggcatcg
      301 cgatcgagct gatggccagc gtgctgcggc cgctgggatc ggcgctggtc accctaccgc
      361 tcggccccga caatcccggg gagctggccg gcccggcgtt cttcatggtg catccgtccc
      421 agttcatcct gccgcaccgc gaggcggcct ggaaggtcat cgcccagcgg ctgggcgaga
      481 tcgccgacgc ctgcgccgaa ctccggcacg agtccggcct cgccacgatc atcgccggcc
      541 agatcgccca gctggagccg accgtccgcg gcctcgccgc ccagctgtcg cggagcggcg
      601 agaccgcgga gcgtacctcg ggtgcctagg gcagcggctg ccagtccggc tgggcgatcg
      661 ttccgacgct cgcctggtcc acgctcacga tggtcgtccg ggcgacgggg tcgaacagga
      721 agctgtccac cggcgggtcg cccggccagc tcagagtggc gatgaccacg cgctgaccgt
      781 cgggggcgcc ggcggtctcg aacgagcggt agtcgccctc ggtggcgtcg gagtggtcga
      841 ggaagcgggg gttggcgccg ttcaggtcga ccaccaccgg gttgtagctc tggacgctga
      901 cgcagtcgcc gacgtcgaag cacttggcca tgaacagcac gtgcaggccg tcgggcatga
      961 agtcggggtt ggtgaccggg ccgttcgggc cgacgccccc gtcctcgatc aggacgcgct
     1021 cggctccgct gctcagggta accagcagga tgcgattgtg ggtgcagccg ctgccggtgt
     1081 tgatgagctg gtggtagacg atcaagccgc cgagcggcga ccaggtgggc cggtcgtcgc
     1141 cgcaggtggt ggcggcatcg tgggtgatcc gggtgatcgc gccgccggtg gcggcgacgg
     1201 tgaagatgtc cccgtggccg ttcgagagca cgttgacgaa ggcgagccgc ttgccgttcg
     1261 gcgaccaggt gggctgatgc gcccgcccgg tggtggtgac ccggcggacc agggtgcccg
     1321 tcgaggtcat gatgtagatg tccttgctgc gctcgaaggc gatctgcttg ccgtccggcg
     1381 accagcgagg gtgccagctc gtgccgtcgg aggtgagccg gcgattcccg gtgccgtcgg
     1441 tgtttaccgt ccagaggtcg tggctggtgc cgctcggact ggtgatgacg taggcgacct
     1501 tgccgtcgtg gcctgggtag gccgcctgcg ccgctggtga ggccgcgagc ccgatcccgg
     1561 ccgcgagcac caacattcct gcgacaaggg caacaatccg gtgcctcacg ccgcatcccc
     1621 tccccccggg gtcagccgcg gcggcccgct ctcgacatcg gtcccgcgtg ctgaggccgc
     1681 cgcgtgaacc tctcaaggct gacggatcga gcgcagccat cgcatgcagc gatcgccgca
     1741 aagcggccta ccgccgttgt gtgacagtgg gtacggtcag gaggattgcg cggcggactc
     1801 gccatctggc ccgtgatcac gatgcgccgg ccagcggcgg cgtccggctt cggagcctcg
     1861 ctttgaagag acgcgctgag tccagctgag cggcttgagt tgggacatcc tccccaggtg
     1921 gtaggcagcg gtgtggcaac tactcccagc gcggtacgcc agaacggtct cgccgggcga
     1981 cgccatctgg gcggcgctcc gccaccatga ctcgtgaccc ggaacgaccc gtcaggacga
     2041 aacgtcagga ggtggcgatg accgagatct cgcatcgcga ccaggtgctg ggcagcgcct
     2101 ttcgcgtcct cgtcatgctg gaggccctgt cgttcctcgt cttcgccgtc ttgcacctgg
     2161 gcgtccggat cccgctcggg ttcgccgtga tcagcaccga cacgatcgac gacgcggtga
     2221 tcgccgaagg cctcagcggg gcgttcctgg cgctgagcgc cgccgcgctg ttcacgcgaa
     2281 gacgctgggc gctgcgggcg acggtgttcg cgcaggcgtt ctccctggca gccgtcctgg
     2341 tcggcatcgc ggccatcgcc gccggtctcg ggccgagcag cctggagaat gacgtctacc
     2401 accgcgtcat ggccccggtg ctggccctgt cgctcgtctg gctggcgacc ccctccggac
     2461 gcggagcact tcaactgtca ggggaggatg cgacgctgcg acgatgacag catccacctg
     2521 gcaccagttc gccaccgagg cgcccgagct ggccacggcc gtccgggcac gcctggaggc
     2581 gaccaagcat cacgtcctcg ccaccctgcg gcgcgacggc tcgccccggg tcagcggcac
     2641 cgaggtgacc ttctttggcg cggatctcat ggtcggctcc atgtggggcg cggtgaaagc
     2701 ccgcgacctg cagcgggacg ggcgcttcgc cctgcacgcc aatccgggcg acggctcgat
     2761 ggagggtggc gacgccaagc tgtccggacg agcggtcgag atcaccgacc ccaacgagct
     2821 ccgcgccttc accaaggccg aggagccgcc ggagccctac catctcttcc ggctgctgct
     2881 cgaccaggcg gtcctcacct cggtcgccaa cgaccgcctg gtgatcgcga gctggcgtcc
     2941 cggcg
//
LOCUS       DASWDC010000111         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_27919, whole genome shotgun sequence.
ACCESSION   DASWDC010000111 DASWDC010000000
VERSION     DASWDC010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27919"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VF995_03550"
     CDS             <1..797
                     /locus_tag="VF995_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9342676.1"
                     /translation="TSVAHGRFLNPATAREPVAVLGAAAAGRLGIDRLYPGERIWVGQ
                     TPRIGQWFYLAGILHPAILTPEIDTAVLIGFPAAQTYLGFDGHPSQIYLRAAIDQVDA
                     VHGVLAATANPEHPGQVNVSQPSDALVARAQAKAALNGLFLGLGAVSLLVGAVGVANT
                     MIISVLERRSEIGLRRALGATKGHIRTQFLSEAMLLATLGGAAGLAAGAAATAIYAHT
                     KHWSTVIPTLAWAGGLAAALLIGALAGLLPALRAARMSPTQALWTV"
     gene            complement(1031..1447)
                     /locus_tag="VF995_03555"
     CDS             complement(1031..1447)
                     /locus_tag="VF995_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006826328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX9342677.1"
                     /translation="MNVEAINIGPSEALAPVSSVSVAAGKGLKGDRHFREDGDDPDRA
                     LTLIEAEVLEDVGLSGAQSRRQVVVRGVQLNDLVGRRFRVGTVECLGTKLCEPCQHLQ
                     SLTRPGIINDLVHRGGLTAQILNDGLISVGDAVVIA"
     gene            complement(1513..>2196)
                     /locus_tag="VF995_03560"
     CDS             complement(1513..>2196)
                     /locus_tag="VF995_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616753.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEX9342678.1"
                     /translation="AEAAGFDLVELHCAHGYLLSSFVSPLTNQRRDRYGGSLAKRLRY
                     PLEVFAAMRAVWPSGKPMTVRISATDWAEGGITGTDAVAIARAFKAVGAAAIDVSTGQ
                     VSPDEQPGFGRSYQTPFADAIRNQVGIATIAVGAISSYDDVNSIVLAGRADLCALGRV
                     HLYDPNWTLHAAVEQDYDGPGVSWPLPWQAGRRKPQTSRSDGPRPRLQLIREGPTGTR
                     HTRWRPTAR"
BASE COUNT          328 a          836 c          728 g          304 t
ORIGIN      
        1 gcacctcggt ggcccacggc cggttcctca acccggccac cgcccgcgag ccggtcgcgg
       61 tgctcggcgc cgccgccgcc gggcggctgg gtatcgaccg gctctaccca ggcgagcgga
      121 tctgggtcgg ccagacgccg aggatcggcc agtggttcta cctggccggc atcctgcacc
      181 cggccatact cacccccgaa atcgacaccg ccgtgctcat cggcttcccc gccgcccaga
      241 cctacctcgg cttcgacggc cacccctcgc agatctacct acgcgcggcc atcgaccagg
      301 tcgacgccgt ccacggcgtg ctcgccgcca ccgccaaccc cgaacacccc ggccaggtca
      361 acgtctccca accctccgac gcgctggtcg cccgcgccca agccaaggct gcgctcaacg
      421 gcctgttcct cggcctgggc gccgtgtccc tgctcgtcgg cgccgtcggc gtcgccaaca
      481 ccatgatcat ctccgtgctg gagcgacgct ccgagatcgg gctacgccgc gccctcggcg
      541 ccaccaaagg ccacatccgc acccaattcc tgtccgaagc catgctgctc gccaccctcg
      601 gcggcgcggc cggcctcgcc gccggcgccg ccgccaccgc catctacgcc cacaccaaac
      661 actggtccac cgtcatcccc accctcgcct gggccggcgg gctggccgcc gccctgctca
      721 tcggcgccct cgccggccta ctccccgcgt tgcgggccgc acggatgtca cccacccaag
      781 cgctctggac cgtataggag gcaggcgatc ggaaagcagg ccgtcccgag cacatcgacg
      841 accaggcaca gccacgacca acccctgagg agggcatgat gaacgacagc acccgcgtca
      901 cacgccggcc acgacgggca cccccgaaga agccctcgac tgcgcctgcc gcctctacct
      961 gaccgaccca accgcttggt catcggatac aacaccgacg aattaacaag cggaaccact
     1021 agtggcgcct ctaggcgatg acgacggcgt ccccgacgga gatcaagccg tcgttgagga
     1081 tctgcgcggt gagcccgccc cggtggacca ggtcgttgat gatcccgggc cgtgtcagcg
     1141 actgcaggtg ctggcacggc tcgcagagct tggtgccgag gcactccacc gtgccgaccc
     1201 ggaagcgccg gccgaccaga tcgttgagct ggaccccgcg caccaccacc tgccggcgcg
     1261 actgggcgcc gctgaggccg acgtcctcca ggacctccgc ctcgatcagc gtcagcgccc
     1321 gatccgggtc gtcgccgtcc tcacggaaat ggcggtcgcc tttgagtcct ttgcccgccg
     1381 ccacgctgac ggagctgacc ggcgcgagtg cttcgctcgg tccgatgttg attgcttcga
     1441 cgttcatcag ccgattgtgc ctagccggct accgcctcgc cacgcaatta ccagcgccga
     1501 cctcctccgg cctcacctgg cggttgggcg ccagcgggtg tggcgggtgc cggtggggcc
     1561 ctcgcggatc agctgcaggc ggggacgggg gccgtcgctg cggctggtct ggggtttacg
     1621 gcgaccggcc tgccagggca gcggccagct gacgccgggg ccgtcgtagt cctgctcgac
     1681 ggcggcgtgc agggtccagt tggggtcgta gaggtggacg cgaccgagcg cgcagaggtc
     1741 ggcgcggccc gcgaggacga tcgagttgac gtcgtcgtag gacgagatcg cccccaccgc
     1801 gatggtcgcg atgccgacct ggttgcggat cgcgtcggcg aagggcgtct ggtacgagcg
     1861 gccgaagccg ggttgctcgt cgggggagac ctggccggtc gagacgtcga tggcggcggc
     1921 gccgacggcc ttgaaggcac gggcgatcgc caccgcgtcg gtgccggtaa tgccgccctc
     1981 ggcccagtcg gtggcggaga tgcgcacggt catcggcttg ccggacggcc agacggcgcg
     2041 catggcggca aacacctcca gcgggtagcg cagccgcttg gcgagcgacc cgccgtagcg
     2101 gtcgcggcgc tggttggtga gcggcgagac gaacgacgac agcaggtagc cgtgggcgca
     2161 gtgcagctcc accaggtcga acccggccgc ctccgc
//
LOCUS       DASWDC010000112         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_528775, whole genome shotgun sequence.
ACCESSION   DASWDC010000112 DASWDC010000000
VERSION     DASWDC010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528775"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VF995_03565"
     CDS             complement(<1..1374)
                     /locus_tag="VF995_03565"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX9342679.1"
                     /translation="MAVHHIQGAVGGREMYLETGKLAQQAGGAVVTGIGETQVLVTAT
                     AGKSPKEQLDFFPLTVDVEERMYAAGKIPGGFFRREGRPSETAILTARLIDRPLRPSF
                     PKGLRNEIHVVATIISADMENNPDIAAINGASAAVQLAGIPFSGPVGAVRLGRHGTDW
                     LVNPTYDELGECTFDMVVAGRQNESGGVDILMVEAEATEHAIALMEDGAPKPTEEALG
                     LALELAKPHIATACQLQLALVHEAGVQHKEYPLFSEYGEDVAGAVRGAVEAQAREAIT
                     IADKAAREARLDELKAEGVQAATDQLGEALAGRERELSAAFRSLTKELVRQRVVKEAK
                     RIDGRGPRDIRPLEAEVGIVPRAHGSGLFQRGETQVLNITTLGMVRMEQIVDTLDPED
                     RKRYMHHYNFPPFSTGETGFMRGPKRREIGHGALAERALLPVIPDKEEFPYALRLVSE
                     VLSSNGST"
     gene            complement(1764..2030)
                     /gene="rpsO"
                     /locus_tag="VF995_03570"
     CDS             complement(1764..2030)
                     /gene="rpsO"
                     /locus_tag="VF995_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX9342680.1"
                     /translation="MLEKVAKTKIITDYATKAGDTGSPEVQVALLTERINQLTEHLRA
                     HKHDHHSRRGLLQMVGRRRRLLRYLRGNDVARYRALIERLGLRR"
     gene            complement(2163..>2642)
                     /locus_tag="VF995_03575"
     CDS             complement(2163..>2642)
                     /locus_tag="VF995_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEX9342681.1"
                     /translation="SSTRIRAELVAGDVQAAARLLDRPFTLEGHVVPGHRRGRLLGIP
                     TANLAVPARLAVPAIGIYAGHLAAEGLGGFGGAPDASGWLPAVTSVGVRPQFGGTELS
                     VETYVLDFEGDLYGRRVTVSFEHRLRGEATFPSVADLVAQMQEDVRQARHLLGMTGR"
BASE COUNT          368 a          889 c          956 g          429 t
ORIGIN      
        1 ggtggagccg ttggacgaca gcacctcact gaccagccgc agcgcgtagg ggaactcctc
       61 cttgtcgggg atcaccggca gcagcgcccg ctcggcgagg gcgccgtggc cgatctcgcg
      121 gcgcttgggg ccgcgcatga aaccggtctc gccggtggag aagggcggga agttgtagtg
      181 gtgcatgtag cgcttgcggt cctcggggtc gagggtgtcg acgatctgct ccatgcggac
      241 catgcccagg gtggtgatgt tgagcacctg ggtctcaccg cgctggaaca gcccagagcc
      301 gtgagcgcgc ggcacgatgc cgacctcggc ctccagcggc cggatgtcgc gtggcccgcg
      361 gccgtcgatg cgcttggcct ccttgactac gcgctggcgg accagctcct tggtgaggct
      421 gcggaacgcg gccgacagct cccgctcccg gccggccagc gcctcgccga gctggtcggt
      481 ggcggcctgg acgccctcgg ccttgagctc gtccaggcgc gcctcgcggg cggccttgtc
      541 ggcgatggtg atcgcctcgc gggcctgcgc ctcgacggcg ccgcggacgg ccccggcgac
      601 gtcctcgccg tactcgctga acagcgggta ctccttgtgc tggacccccg cctcgtgcac
      661 cagcgccagc tgcagctggc aggcggtggc gatgtgcggc ttggccagct caagggcgag
      721 gccgagcgcc tcctcggtgg gcttgggggc gccgtcctcc atcagggcaa tggcgtgctc
      781 ggtggcctcg gcctcgacca tcaggatgtc gactccgccg gactcgttct ggcgccccgc
      841 gaccaccatg tcgaaggtac attctccgag ttcgtcgtag gtcgggttga ccagccagtc
      901 agtgccgtgg cggccaaggc ggacagcgcc caccgggccg gagaagggga tgccggcgag
      961 ctgcacggcg gccgacgcgc cgttgatggc ggcgatatcg gggttgttct ccatgtcggc
     1021 ggagatgatc gtggcgacca cgtggatctc gttgcgcagg cccttcggga acgacgggcg
     1081 cagcgggcgg tcgatcaggc gtgcggtgag gatggcggtc tcggacggcc gcccctcacg
     1141 acgaaagaag ccgccgggga tcttgccggc ggcgtacatc cgctcctcga cgtcgacggt
     1201 gagcgggaag aagtcgagct gttccttggg gctcttgccg gcggtggcgg tgaccaggac
     1261 ctgggtctca ccgatgccgg tgaccacggc gccgccagcc tgctgggcca gcttgccggt
     1321 ttccaggtac atctcacggc cgccgacggc gccctggatg tgatgaactg ccatgaagtt
     1381 gttgtctcct tgtcgtcatt cgcccgcgag gcttcagatc cgcgactcgg cgggcgatgc
     1441 tctggtggtt ccgcaggccg gacctcggcg aattcagcgg acaccgtcct gggacgcggc
     1501 caattcccgg ttttggttcc cgaatctcgg gctgccgcga ggcgagcctg acgagtgatc
     1561 agcgagcggc ggaggtgcca gttctcagtc gtaaggcctg gaggcccgcg gagaaaatcc
     1621 gcgcatccag ggcttaccac tgacaactgg cacgggcggt cggcaccagg cccgtcatgc
     1681 tcgggtcagt gcaccagggg cgacaccgcc tctcctcgtc ggtggaactc cttgtcctgt
     1741 cgggtcgtgg ccgtcagttg gcgtcagcgg cgcaggccga gccgctcgat cagcgcccgg
     1801 tagcgggcga cgtcgttgcc gcgcagatag cggagcagcc gccgacggcg gccgaccatc
     1861 tggagcagcc cacggcgcga gtgatggtcg tgcttgtggg cccgcaggtg ctcggtgagc
     1921 tggttgatcc gctcggtgag cagcgccacc tggacctcgg gcgagccggt gtcgcccgct
     1981 ttggtggcat agtccgtaat gatctttgtc ttggcaacct tctccagcat gtgccgcttt
     2041 ccttcctgaa cggtgcggaa tctgcctggg ggcaccgggg cgctgtggcg cccacgaagt
     2101 cccgcatccc aggcggcagc cggaatcaca caagtgaagc cagccgcccg tagtacagat
     2161 tatcaccgcc ctgtcatgcc gagcagatgc cgcgcttggc gcacgtcctc ctgcatctgc
     2221 gccaccaggt cggcgacgct cgggaaggtc gcctcgccgc gcaggcggtg ctcgaacgag
     2281 acagtgaccc gccgcccgta caggtcgcct tcgaagtcga gcacgtaggt ctcgacgctc
     2341 agctcggtcc cgccgaactg gggccgcacg ccgaccgagg tgaccgcggg gagccagcct
     2401 gacgcatcgg gggctccccc gaacccccca aggccctcgg ccgccagatg gccggcgtag
     2461 atcccgatcg ccgggaccgc caggcgtgcc ggcaccgcca ggttcgcggt cgggatcccg
     2521 agcaggcgac cgcgccggtg ccccgggacc acgtggccct ccagtgtgaa ggggcggtcg
     2581 agtaggcggg ctgcggcctg gacgtcgccg gcgaccagct ccgcgcggat ccgggtcgag
     2641 ga
//
LOCUS       DASWDC010000113         2521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1362458, whole genome shotgun sequence.
ACCESSION   DASWDC010000113 DASWDC010000000
VERSION     DASWDC010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362458"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VF995_03580"
     CDS             complement(<1..343)
                     /locus_tag="VF995_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342682.1"
                     /translation="MGPYHTLLAAASVQGALGAVGRSLWSLISGLAVITILTTIVGRL
                     LGVRLRWWRALAAGFLGIIAGYLFDWAITGHPRNPHFSPGVIALSAVPATMVIAVMME
                     LLARPGRFANVQ"
     gene            complement(447..1349)
                     /locus_tag="VF995_03585"
     CDS             complement(447..1349)
                     /locus_tag="VF995_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9342683.1"
                     /translation="MSPAQGRKGAPEDNGLAGDGLDPVLAWAEEAVDFLGRRLTGRYA
                     VDEFGYDRDLVEHVIAPLLRPLYRRWWRVQVRGLEHVPEKGAALVVGNHAGTLPFDAM
                     MVALAIYDEHPAHRVLRMLAADLAFTLPLVAPLARKSGNTLACGEDAERLLRGGELVG
                     VWPEGYKGLGKPFRERYRLQRFGRGGFVGLALRHRVPIVPVAVVGGEEIYPMVGNLRR
                     LARVLGFPYLPVTPTFPLLGPLGLVPLPSKWLIEFCPPLDTSAFPPDAADDPMVVFDL
                     TDQVRDTIQQALNRNLTERDGVFH"
     gene            complement(1346..>2521)
                     /locus_tag="VF995_03590"
     CDS             complement(1346..>2521)
                     /locus_tag="VF995_03590"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9342684.1"
                     /translation="LMAKAAAAAMKPARRVLVTGAARSTGRRLVAALAAEKGIAKVIG
                     VDMDLPAEPFPLPDAGEKGRRGRRSGKIEFLAGGLRSQALTEAVRTSGIDTLVHLGAT
                     AEPLAAGGRATMKDQNVLGTMQLLAAAYHAPELRRVVLKSTTAVYGSAPDDPAVWTEG
                     MPHETPRSGFGKDAIEVEDSVRSFQRRRPDVAVTVLRLANQVGPTVSNPLTRYFELLA
                     CPVVFGFDPRLQFLHEDDTQRVLRRAVLGQAAAGTFNLAGDGVVLLSQAVRIAGKPPL
                     PIPEPLVGLAAELLRRTIDFSPEQVRFLQFGRVADTGKAHERLGFTPEWSSLAAFEDF
                     ASRLPHLVTEERVAEAQKAALDTLARAATWLRRFAGAAGAAGSAARTVGARGSGAGS"
BASE COUNT          366 a          913 c          935 g          307 t
ORIGIN      
        1 actggacgtt ggcgaagcgg ccggggcggg ccagcaactc catcatgacg gcgatcacca
       61 tggtcgcggg gaccgccgag agggcgatca cccccggcga gaaatgcggg ttccggggat
      121 gccccgtgat tgcccagtcg aacaggtagc ccgcgatgat gccgaggaag cccgcggcga
      181 gcgcccgcca ccagcgcagc cggaccccca gcaggcgccc gacgatcgtc gtcaggatgg
      241 tgatcaccgc caggccggag atcaggctcc agagggaccg cccgaccgct ccgagggcgc
      301 cctgcacgga ggcggcggcg agcaacgtgt ggtagggacc catcagacac ccttccggtc
      361 aacggttggc gaagtccccc catgcgaagc taccgcgtcg ctggaccgac ggcacagcca
      421 ggttatgggc cgacgccgac cgcgggtcag tggaagacgc cgtcgcgctc ggtgaggttg
      481 cggttgagcg cctgctggat ggtgtcgcgg acctggtcgg tgaggtcgaa gaccaccatc
      541 gggtcgtcgg ccgcgtccgg cgggaaggcg ctggtgtcca gcggcgggca gaactcgatc
      601 agccacttgc tgggcagcgg caccaggccg agcggcccga gcagcgggaa ggtcggcgtc
      661 accggcaggt aggggaagcc gagcacgcgg gcgaggcggc gcaggttgcc gaccatcggg
      721 tagatctcct ccccgccgac caccgccacc ggcacgatcg ggacgcggtg gcgcagcgcc
      781 aggccgacga agccgccgcg cccgaaacgc tgcagcctgt agcgctcccg gaagggcttg
      841 ccgagaccct tgtagccctc cggccagacg ccgaccagct cgccgccgcg cagcagccgc
      901 tcggcgtcct cgccgcaggc cagggtgttg ccggacttgc gggccagcgg cgccaccagc
      961 ggcagggtga aggccaggtc ggcggccagc atgcgcagga cccggtgggc gggatgctcg
     1021 tcgtagatcg ccagggcgac catcatcgcg tcgaacggca gcgtgccggc gtggttgccg
     1081 accaccaggg cggcgccctt ctccggcacg tgctcgaggc cgcggacctg cacccgccac
     1141 cagcgccggt acagcggccg cagcagcggc gcgatcacgt gctcgaccag gtcgcggtcg
     1201 tagccgaact cgtccaccgc atagcggccg gtgaggcgcc ggccgaggaa gtcgaccgct
     1261 tcctccgccc aggccaggac cgggtcgagc ccgtcgccag cgagcccgtt atcctccggc
     1321 gcgcccttcc ggccctgggc cgggctcacg atcctgcccc tgagcctctt gcccccaccg
     1381 tcctggccgc cgacccggcc gctccggccg ccccggcgaa gcgccgcagc caggtggcgg
     1441 cgcgggcgag ggtgtcgagg gcggccttct gcgcctcggc cacacgttcc tcggtgacca
     1501 ggtgcgggag gcggctcgcg aagtcctcga aggcggccag cgacgaccac tccggggtga
     1561 agccgagccg ctcgtgggcc ttgccggtgt cggccacccg gccgaactgc aggaagcgga
     1621 cctgctccgg ggagaagtcg atggtgcgcc gcagcagctc ggccgcgagc ccgaccagcg
     1681 gctccgggat cggcaggggc ggcttgccgg cgatccggac cgcctggctg agcagcacca
     1741 ctccgtcgcc ggccaggttg aaggtgccgg ccgccgcctg gccgagcacc gcgcggcgca
     1801 gcacccgctg ggtgtcgtcc tcgtgaagga actgcaggcg cgggtcgaag ccgaacacca
     1861 ccgggcaggc cagcagctcg aagtagcggg tgagcggatt cgagacggtt gggccgacct
     1921 ggttggcgag ccgcagcacg gtgacggcca cgtcgggccg gcgccgctgg aacgagcgga
     1981 cggagtcctc gacctcgatg gcgtccttgc caaaaccgga ccggggtgtc tcgtggggca
     2041 tcccctcggt ccacaccgcc gggtcgtcgg gcgcggagcc atagaccgcc gtggtcgact
     2101 tgagcaccac ccgccgcagc tcgggcgcgt ggtaggcggc ggccaggagc tgcatcgtgc
     2161 cgagcacgtt ctggtccttc atggtggccc ggcccccggc tgccagcggc tcggcggtgg
     2221 cgccgagatg gaccagtgtg tcgatcccgg aggtccgcac cgcctcggtc agcgcctggc
     2281 tgcgcaggcc cccggcgagg aattcgatct tgccgctcct gcggccccgg cggcccttct
     2341 cgccagcgtc ggggagcggg aacggctcgg ccgggaggtc catgtcgacg ccgatcacct
     2401 tggcgatgcc cttctcagcc gccagggccg cgaccagccg ccggccggtc gagcgcgccg
     2461 cccccgtcac caggacgcgg cgcgccggct tcatcgcagc agcggcggcc ttggccatca
     2521 g
//
LOCUS       DASWDC010000114         8229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_432011, whole genome shotgun sequence.
ACCESSION   DASWDC010000114 DASWDC010000000
VERSION     DASWDC010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432011"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VF995_03595"
     CDS             complement(<1..1164)
                     /locus_tag="VF995_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9342685.1"
                     /translation="MGVRGRGVGVVAVLTLVLTLAACSGKSSSGGSGGSSGDTIKIGA
                     SIPISGGLASFGFFEKWGYQHAVNAINAAGGIEVDGKKKKIDLILLDDATDPNQVVNN
                     IQRLITSDRVDALLGSCTNSLVEPGALVADRASVPFVTGCATTNGFEAAKKWTWAWDL
                     FFNSEQLTETPFNTVDELGLQTNKKVAILHSNGKSENDIGATEWPKWAKKHGWTVTFN
                     QAFPPGTTQFNSALQSIKSSGADMLLSVFPPPASLAIRKQMISAGYNPKLLVQEEGGE
                     PVQYAQALGSQANGVMVGGFWDPSFPYPGAQDLRKLFEAQTKQTFSQHIADTEAAAQI
                     LLDAFKRAGTTDKTKLNAAIGQTDAMYVVGQVKFDANHTYTLPMVEEQWQNGKN"
     gene            complement(1370..3331)
                     /locus_tag="VF995_03600"
     CDS             complement(1370..3331)
                     /locus_tag="VF995_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9342686.1"
                     /translation="MTGVAPYGSWKSPISAEQLARGGVGLGELQTDGDAVLWLELRPA
                     EGGRQVVCRWEGGAVAQLTPDGFNVRTRVHEYGGGAFLARRGVVWFSNFADQRLYRQD
                     PDASPRPITPEPAVPAALRYADACVTPDGALLVCVRERHEDGAVTNELVALPADGSAE
                     PRVISGGRDFYASPRLAADGRQLAWLEWDHPNMPWDGTELRVAEVEREPGSGQLRLAG
                     AKLVAGGSEESVFQPAWSADGELYFVSDRTGWWNLYRLAQGGEAEPLAPMEAEFGLPQ
                     WVFGMTSYALLPGGRIACVYGRGPVRSLGVLAPGSVRPDPIQLPYTSFTGLHALGGSL
                     AAGTVACVAGSPALAPEVALIDLDSGAATTLRRSREVDFDPAYLSSPQPIEFPTEGGQ
                     TAHALYYPPANPAFQAPEGERPPLLVVSHGGPTAQASSVLNVGVQFFTSRGFAVCDVD
                     YGGSTGYGRAYRQRLNGRWGVVDMADCANAALYLAGQGEADSARLAIRGGSAGGYTTL
                     CALTFRDDFAAGASYFGVADLAALAQDTHKFESRYLDRLIGPYPEAAVVYYERSPIRY
                     TERLSCPVILLQGLEDMVVPPAQAEAMAAALDAKGIPYAYLPFEGEQHGFRRAEHIQR
                     AAEAELYFYSRIFGFELADPVEPVEIHNL"
     gene            complement(3462..3818)
                     /locus_tag="VF995_03605"
     CDS             complement(3462..3818)
                     /locus_tag="VF995_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX9342687.1"
                     /translation="MTAIADPDCLFCRIVAKDIPADVVLDRDDVLAFRDVNPQAPTHV
                     LVIPKEHIPSLEAVVAEHGELLASLVGAINEVAAADGISGGFRVVTNIGPSAGQSVDH
                     LHLHVLGGRMFGWPPG"
     gene            complement(3815..5179)
                     /gene="mtaB"
                     /locus_tag="VF995_03610"
     CDS             complement(3815..5179)
                     /gene="mtaB"
                     /locus_tag="VF995_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX9342688.1"
                     /translation="MTHRGAPRPPGDRLPVLPATGPCPPNPPKVAFKTLGCRLNQAES
                     EKALEALGAQGFDLAHAGEEPDVVVINTCTVTVESTASSRRLIRKTAAAHPGATVVVT
                     GCYAVSDPEAVRAIPGVDLVVDNDGKDRIGELIVAVPKVRRLPLMVRPAPAERLRTRV
                     AIKAQTGCDEWCTFCIIPRTRGPLKSYPEDEIVADVRRQVARGVREVVLTGVHLGKYG
                     DDRGERDGLARLVDRLTAIDGLLRLRLSSVLPVQVTPALVERVRDDPKVCRHLHVPLQ
                     AGDDQVLAAMHRPYRIAEVLERVEHAKAEIPSLGLSTDIIVGFPGETAAQFAATLAVT
                     ERVGFSKLHVFRYSPRPGTPAATMADQVADVDKKARARELIALGGELRRRFHEQHLGS
                     LLAVLVESAEGSADGEGLAEGTSDNYIKVRFPWRGGPELSGQLVTVRGIRADNEGMEG
                     ELVA"
     gene            complement(5187..6011)
                     /locus_tag="VF995_03615"
     CDS             complement(5187..6011)
                     /locus_tag="VF995_03615"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX9342689.1"
                     /translation="MSARPLTLLGCFAHPDDETWSMSGSFALLAPKGVHCAVWTATRG
                     EAGEIAPETGVTRERLAEVREAEERAALAVLGVEDATFGDFADGKVSERADDLVRAIQ
                     VTLDRVQPDVVVTMEPNGVTAHADHMAVSAATRTAFSAYVALRGNGELERDVREPRLY
                     YWGMPNSKLDRFREIGAEIGMEIPAEGEPFAPRGTPDEQFTCHVDTAAVVDLAVRALR
                     QHRTQMGGFYALLDRPDLLADVFDVVDFIRIHPAPQPGDPPESSLLEAFLGGPASA"
     gene            complement(6070..6558)
                     /locus_tag="VF995_03620"
     CDS             complement(6070..6558)
                     /locus_tag="VF995_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9342690.1"
                     /translation="MATYAEQVGERLRNIRLQKGLSLHDVEEGSSKEFKASVLGAYER
                     GERSISVPRLQRLAMFYDVPVDHLLPKLGPPPGGEVAVPAEGDSVCIDLEALEGRREP
                     EARTLVRYVSLIQMQRGDFNGKMLTIRWDDVRVLAAVLDQSPGGLVGRLAELGLRRPS
                     HN"
     gene            complement(6767..7726)
                     /gene="nrfD"
                     /locus_tag="VF995_03625"
     CDS             complement(6767..7726)
                     /gene="nrfD"
                     /locus_tag="VF995_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX9342691.1"
                     /translation="MTTTRGERVGASSGPGGRPGVNMVGKAEFRSYYGRPILKEPVWT
                     WEIPWYFFLGGIAGASAPLAFAARLAGNHRLARAALTTGAAALAASPALLVSDLGRPE
                     RFHHMLRVFRPTSPMNMGSWLLAALSPPLLGAAAADRLGVAPRLARGAEGAAAVLGPG
                     LATYTAVLVADTAVPVWHEAGKELPFVFAGSAAAAAGAAACLLTPPEHAGPARRLAIG
                     GAAAELAAGAVMERRLGELAEPYGQPPVRRLARLAKGLGVAGALTLGLAGRRRFGATV
                     GAALLLASSACERQAVVGAGRLSARDPKYVVKPQRERLAQRSR"
     gene            complement(7723..>8229)
                     /locus_tag="VF995_03630"
     CDS             complement(7723..>8229)
                     /locus_tag="VF995_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9342692.1"
                     /translation="ALFRTEIGSVVVQPDICNGCGYCVPACPFGVIDRREDDGRAWKC
                     TLCYDRSRDGLEPACAKACPTDSIQYGPLDELRERADRRLDTLRERGVESASLYLADE
                     ANGVGGAGAFFLLLDEPEVYGLPPDPVSTTRDLGGLWKAAAMAAGALLAGVAIAFAGG
                     GNAKGGRR"
BASE COUNT         1150 a         2884 c         3016 g         1179 t
ORIGIN      
        1 gttcttgccg ttctgccact gctcctcgac catcggcagc gtgtaggtgt ggttggcgtc
       61 gaacttgacc tggccgacca cgtacatggc gtcggtctgc ccgatcgccg cgttcagctt
      121 ggtcttgtcg gtggtgccgg cgcgcttgaa ggcgtcgagc aggatctgcg ccgccgcctc
      181 ggtgtcggcg atgtgctgcg agaacgtctg cttggtctgg gcctcgaaca gcttgcgcag
      241 gtcctgcgcg ccggggtacg ggaacgacgg gtcccagaag ccgcccacca tcacgccgtt
      301 ggcctggctg ccgagcgcct gggcgtactg gaccggctcg ccgccctcct cctgcacgag
      361 gagcttgggg ttgtagccgg ccgagatcat ctgcttgcgg atcgcgagcg aggccggcgg
      421 cgggaacacg ctcaggagca tgtcggcgcc ggacgacttg atgctctgga gcgccgagtt
      481 gaactgggtg gtcccgggcg ggaaggcctg gttgaaggtg accgtccagc cgtgcttctt
      541 ggcccatttc ggccattccg tggcgccgat gtcgttctcg ctcttgccgt tggagtgcag
      601 gatcgcgacc ttcttgttgg tctgcaggcc caactcgtca acggtgttga aaggggtctc
      661 ggtgagctgc tcggagttga agaacaggtc ccaggcccag gtccacttct tggccgcctc
      721 gaagccgttg gtggtggcgc agccggtgac gaacggcacg ctggcccggt cggcgaccag
      781 cgcgccaggc tcgaccagcg aattggtgca ggagccgagc agcgcgtcga cccggtcgct
      841 ggtgatcagc cgctggatgt tgttgaccac ctggttgggg tcggtggcgt cgtcgagcag
      901 gatcaggtcg atcttcttct tcttcccgtc gacctcgatg ccgccggcgg cgttgatcgc
      961 gttgaccgcg tgctggtagc cccacttctc gaagaagccg aagcttgcga gcccgccgct
     1021 gatcgggatc gacgcgccga tcttgatcgt gtcgcccgag gagccgccgc tcccgccgga
     1081 cgagctcttg ccgctgcatg cggcgagcgt gagcaccagc gtgagcaccg cgaccacgcc
     1141 gacgcctcgg cccctaactc ccatcaaggt ccacctccgg gttgcttcct cgccgacggg
     1201 accgccctga tacggtgtcc gcctgcccgc aatgtggata gacagttcca actagtggga
     1261 cggctgacgg taatcagcga gcggacccgc tgtcaagacc caagcgagcc aagggaccat
     1321 ggccccgaca acctgggagc cagcggtctg tctggaggcg gcggcgcgct cagaggttgt
     1381 ggatctcgac cggctcgacc gggtcggcca gctcgaagcc gaagatgcgg ctgtagaagt
     1441 acagctccgc ctcggcggcg cgctggatgt gctcggcgcg gcggaagccg tgctgctcgc
     1501 cctcgaacgg caggtaggcg taggggatcc ccttggcgtc cagggccgcc gccatggctt
     1561 cggcctgggc gggcgggacg accatgtcct ccaggccctg gagcaggatc accgggcacg
     1621 acagccgctc ggtgtagcgg atcggcgagc gctcgtagta gaccaccgcc gcctccgggt
     1681 aggggccgat caggcggtcc aggtagcgcg actcgaactt gtgggtgtcc tgcgccagcg
     1741 ccgccaggtc ggcgacgccg aagtagctcg ccccggcggc gaagtcgtcg cggaaggtga
     1801 gcgcgcacag ggtggtgtag ccgccggcgc tgccgccgcg gatcgccagg cgggcgctgt
     1861 cggcctcgcc ctggccggcc aggtacaggg ccgcgttggc gcagtcggcc atgtcgacga
     1921 ccccccaccg gccgttgagg cgctgccggt aggcgcggcc gtagccggtc gagccgccat
     1981 agtcgacgtc gcacacggcg aacccgcggc tggtgaagaa ctgcacgccg acgttcagca
     2041 cgctgctcgc ctgggcggtc ggcccaccgt ggctgaccac caggaggggc ggccgctcgc
     2101 cttccggagc ctggaacgcc gggttggccg gcgggtagta cagcgcgtgg gcggtctggc
     2161 cgccctcggt ggggaactcg atcggctggg gggatgacag gtaggccggg tcgaagtcga
     2221 cctcgcggct gcggcgcagg gtggtggccg cgccgctgtc caggtcgatc agcgcgactt
     2281 ctggggcgag ggccgggctc ccggccacgc aggcgacggt gccggcggcg aggctgccgc
     2341 cgagtgcgtg caggcccgtg aaactggtgt agggcagctg gatcgggtcg gggcggacac
     2401 tgcccggggc gaggaccccg agggagcgga ccgggccgcg gccgtagaca caggcgatcc
     2461 gcccgccggg cagcagcgcg tagctggtca tgccgaagac ccactgcggc agcccgaact
     2521 cggcctccat cggggcgagc ggctcggcct cgcccccttg ggcgaggcgg tagaggttcc
     2581 accagccggt gcggtcggag acgaagtaca gctccccgtc cgcactccac gctggctgga
     2641 agaccgactc ctcggagccg ccggcgacca gcttcgcccc ggccagccgg agctgccccg
     2701 atcccggctc ccgctcaacc tcggcgacgc gcagctcagt gccgtcccag ggcatgttgg
     2761 ggtggtccca ttcgagccag gcgagctgac ggccgtcggc cgccaggcgc ggagaggcgt
     2821 agaagtcccg gccgccgctg atcacccggg gctccgccga accgtcggcc ggcagggcca
     2881 ccagctcgtt ggtgaccgcg ccgtcctcgt ggcgttcgcg gacgcagacc agcagggcgc
     2941 cgtccggggt cacgcaggcg tccgcgtagc ggagggccgc cgggacagcc ggctcagggg
     3001 tgatcggccg gggcgacgca tcggggtcct ggcggtacag ccgctggtcg gcgaagttgg
     3061 agaaccacac cacgccgcgg cgggcgagga aggcgccgcc gccgtactcg tgcacgcgcg
     3121 tgcgcacgtt gaagccgtcc ggggtgagct gggcgacggc ccccccttcc cagcggcaga
     3181 cgacctggcg gccgccttcg gctggccgca gttccagcca caggacggcg tcgccgtcgg
     3241 tctgcagctc gccgagcccg acgccgccgc gcgcgagctg ctcggcactg atcggcgact
     3301 tccacgagcc gtagggcgcc acgcccgtca tccgctcccc tccttctccg ttctccgttg
     3361 ccgcattgcg aaaacgcccc tcggtcgagg ggggtccccc tgtcaaaagt ccatcacatt
     3421 cggccggtgg cgtgagtcgt tgtccacagg tcaggtccgg gctaaccggg cggccagccg
     3481 aacatgcggc cgccgagcac gtgcaggtgg agatggtcga cggactgccc ggcgctcggc
     3541 ccgatgttgg tgaccacccg gaaccccccg ctgatcccgt cggcggcggc gacctcgttg
     3601 atggcgccga ccagactggc gagcagctcg ccgtgctcgg cgacgaccgc ctccaggctg
     3661 gggatatgct ccttggggat caccagcacg tgggtcgggg cctgcgggtt gacgtcgcgg
     3721 aaggccagca cgtcgtcgcg gtcgaggacc acgtcggcgg ggatgtcctt ggcgacgatc
     3781 ctgcagaaca ggcagtccgg gtcagcgatg gcggtcatgc gaccagttct ccctccatgc
     3841 cttcgttgtc ggcgcggatg cctcgcacgg tcaccagctg gccggacagt tccgggccgc
     3901 cgcgccaggg gaagcggacc ttgatgtagt tgtcgctggt gccctcggcg aggccctcgc
     3961 cgtcggcgga cccttcggcc gactcgacca ggaccgccaa gaggctgccg aggtgctgtt
     4021 cgtggaagcg gcggcgcaac tcgccgccga gggcgatcag ctcgcgggca cgagccttct
     4081 tgtcgacgtc ggcgacctgg tcggccatgg tcgcggcggg ggtgcccggc cgcggtgagt
     4141 agcggaacac gtgcagcttg gagaacccga cccgctcggt gaccgccagg gtggcggcga
     4201 actgcgcggc ggtctcgccg gggaagccga cgatgatgtc ggtcgacagg cccaggctgg
     4261 ggatctcggc cttggcgtgc tcgacccgct ccaggacctc ggcgatgcgg tacgggcggt
     4321 gcatggcggc gagcacctga tcgtcgccgg cctgcagcgg cacgtgcagg tggcggcaga
     4381 ccttggggtc gtcgcgtacc cgctcgacca gcgccggggt gacctggacc ggcaggacgc
     4441 tggagaggcg caaccgcagc agcccgtcga tcgcggtgag gcggtcgacg agccgggcga
     4501 ggccgtcgcg ttcgccccgg tcgtcgccgt atttgccgag gtggacgccg gtgagcacca
     4561 cctcgcggac gccgcgggcg acctggcggc gcacgtcggc gacgatctcg tcctccgggt
     4621 agctcttcag cggcccgcgg gtgcgcggga tgatgcagaa ggtgcaccac tcgtcgcagc
     4681 cagtctgcgc cttgatcgcc acccgggtcc ggagccgctc ggccggcgcg ggacggacca
     4741 tcagcggcag gcggcgcacc ttcgggaccg ccacgatcag ctcgccgatc cggtccttgc
     4801 cgtcgttgtc gaccaccagg tccacaccgg ggatcgcccg gacggcctcg gggtcgctga
     4861 ccgcgtagca gccggtcacc accaccgtcg cgccgggatg ggcggcggcg gtcttgcgga
     4921 tcagccgtcg cgacgacgcg gtcgactcca ccgtgacggt gcaggtgttg atcactacca
     4981 cgtcgggttc ctcgcccgcg tgggccaggt cgaagccctg ggcgccgagg gcctccaggg
     5041 ccttctccga ctcggcctgg ttgaggcggc agccaagggt cttgaaggcg accttcgggg
     5101 ggttcggggg gcatggtccc gtggccggaa ggaccggaag ccgatcccca ggtggccgcg
     5161 gggccccccg atgggtcaag ccctgctcag gcactggctg ggccgccgag gaaggcctcc
     5221 agcagcgacg actcgggcgg gtcgccgggc tgcggggccg ggtggatgcg gatgaagtcc
     5281 accacgtcga agacgtcggc gagcaggtcg ggacggtcga gcagggcgta gaagccgccc
     5341 atctgggtac ggtgctgacg cagcgcgcgg acggcgaggt cgaccacggc ggcggtgtcg
     5401 acgtggcagg tgaactgctc gtcgggggtg ccgcgcgggg cgaacggctc gccttcggcg
     5461 ggaatctcca tgccgatctc ggcgccgatc tcgcggaagc ggtcgagctt cgaattcggc
     5521 atcccccagt agtacaggcg cggctcgcgc acgtcgcgct ccagctcgcc gttgccgcgc
     5581 agcgcgacat aggcgctgaa ggccgtccgg gtcgcggcgc tcaccgccat gtggtcggca
     5641 tgggcggtga cgccgttcgg ctccatggtc accaccacgt cgggctggac ccggtccagg
     5701 gtcacctgga tcgcgcggac caggtcgtcg gcgcgttcgc tgaccttgcc gtcggcgaag
     5761 tcgccgaagg tggcgtcctc gacgccgagc acagccagtg cggcacgctc ctcggcttcg
     5821 cggacctcgg cgaggcgctc ccgcgtcacc ccggtctcgg gggcgatctc gcccgcctcg
     5881 ccccgggtcg ccgtccagac cgcgcagtgg actcccttgg gggccagcag cgcgaacgat
     5941 ccgctcatcg accaggtctc gtcgtcgggg tgcgcgaaac aaccgagcag ggtcagcgga
     6001 cgagcggaca tgcgacctcc agcttcagct ccagcggcca gtcacgggcc agtcacggca
     6061 gatgcggact cagttgtggc ttggacggcg caggcccagc tcggcgagcc gcccgaccag
     6121 gccgccagga gactggtcga gcaccgctgc caggacgcgc acgtcgtccc acctgatggt
     6181 gagcatcttg ccgttgaaat cgccgcgctg catctggatt agggagacat accgtacgag
     6241 ggtacgcgcc tctggctccc gccgaccctc cagcgcctcc aggtcgatgc agaccgagtc
     6301 accctccgcc gggacggcga cttcgccgcc gggcggcggt ccgagcttcg ggagcaggtg
     6361 gtcgacgggc acgtcgtaga acatcgccag ccgctgcagg cgcgggaccg agatgctgcg
     6421 ctcgccgcgc tcgtaggcgc ccagcacgct ggccttgaac tccttgctgg agccctcctc
     6481 aacgtcgtgc agcgagagtc ccttctgtaa ccggatattg cggagccgtt cccccacttg
     6541 ctccgcatag gtcgccatga caacctcccg gctctggttt gcccccctct gggcacattg
     6601 tgggcggaca atagcatcac accctaagct cttcaatcac cgaaggtggc cgaatccgca
     6661 ccattggtgc gggatgttcg agcgtctgga ccctgccaac cgcctgccgc caccgcgcaa
     6721 atcgcaacga cgcggggcga gcgccgaggc gagcgccgaa cggaacctac cgcgaccgct
     6781 gcgccaggcg ctcccgctgc ggcttgacca catatttcgg gtcgcgtgcc gacaagcgcc
     6841 cggcaccgac gaccgcctgg cgttcgcagg cggagctggc cagcagcagc gccgcgccga
     6901 cggtggcacc gaagcggcgc cgcccggcca ggccgagcgt cagcgcaccc gccacgccga
     6961 ggcccttggc gaggcgggcg aggcgacgca cgggcggctg cccgtacggt tcggccagct
     7021 caccgagacg gcgctccatg accgcgccag cggccagctc ggcggcggcg ccgccgatcg
     7081 ccaggcggcg ggcgggcccg gcgtgctcgg gcggggtgag caggcaggcg gcggcgcccg
     7141 cggcggcggc ggcgctgccg gcgaacacga acggcagctc cttgccggcc tcgtgccaga
     7201 ccgggacggc ggtgtcggcg accagcacgg cggtgtaggt ggcaaggccc gggccgagca
     7261 cggcggcagc accctccgca ccacgggcca atctgggcgc cacgccgagc cggtcggcgg
     7321 cggcggcgcc cagcagcggc gggctgagcg cggccagcag ccagctgccc atgttcatcg
     7381 gcgaggtcgg ccggaagacc cgcagcatgt ggtggaagcg ctccggccgg ccgagatcgg
     7441 agaccagcag cgccgggctg gccgccagcg ccgccgcgcc ggtggtgagc gcggcgcgag
     7501 cgagccggtg gttgccggcg aggcgcgccg cgaacgccag cggcgccgac gccccggcga
     7561 tcccgccgag gaagaagtac caggggatct cccaggtcca gaccggctcc ttgaggatcg
     7621 ggcggccgta gtagctgcgg aactccgcct taccgaccat gttgacgccg ggccgcccgc
     7681 ccgggcccga gctggcgccc acccgctccc ctctcgtcgt tgtcacctgc ggcctccctt
     7741 ggcgttgccg ccgcccgcga acgcgatcgc cacgcccgcg agcagagcgc ccgccgccat
     7801 tgccgcggcc ttccagaggc cgccgaggtc gcgggtcgtg ctgaccgggt ccggcggcag
     7861 cccgtagacc tcgggctcgt cgagcagcag gaagaacgcg ccggccccgc cgacgccgtt
     7921 ggcctcgtcg gcaagataca ggctggcgct ctccaccccc cgctcccgca gggtatccag
     7981 ccgccggtcg gcgcgttcgc gcagctcgtc gaggggaccg tactggatgg agtcggtcgg
     8041 gcacgccttg gcgcaggccg gctccagccc gtcccggctg cggtcgtagc acagcgtgca
     8101 cttccacgcc cgcccgtcgt cctcgcgacg gtcgatcacc ccgaaggggc aggcgggcac
     8161 gcagtacccg cagccgttgc agatgtcggg ctgcaccacc accgacccga tctcggtgcg
     8221 aaacagcgc
//
LOCUS       DASWDC010000115         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1613951, whole genome shotgun sequence.
ACCESSION   DASWDC010000115 DASWDC010000000
VERSION     DASWDC010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613951"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..910)
                     /locus_tag="VF995_03635"
     CDS             complement(158..910)
                     /locus_tag="VF995_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9342693.1"
                     /translation="MLGNDPGDGFHADRLERARRLVARSQFELAERELRGVLGHGAGH
                     GGTHALLALCLARQGDLADAEREAAEAVGLDPDGALGHYILAGVLRRRGRFDEAEAAA
                     RKAIVLAPGNADVLAALAQIQLAKRRWAEAAATAAQGLDANPDHRDCANLRAFALIRL
                     GRRAEAATVLAGLLARDPENPRTHANQGWLLLYTGDYDGAAEHFREALRLLPTLEWAR
                     RGLSLTQKAHNPLYRALLRLTLRPSALFGGLL"
     gene            complement(1043..>1843)
                     /locus_tag="VF995_03640"
     CDS             complement(1043..>1843)
                     /locus_tag="VF995_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX9342694.1"
                     /translation="RWSPRDGMIDAAALAGVDAVVNLAGKGIGARPWTAGVKREITES
                     RVRGTRLLAETLAGLARESGGSTGPRVLVVASGAHYYGDRGDESLSEGSSPGQGFLSG
                     VCQAWEAAADPARAAGIRVVHVRTGPVQARGGMLAVLAPLYKLGLGGRLGSGRQWWSW
                     IALDDVVGVYRHAIATEELEGPVDATAPHPVTNAEHSATLARVLGRPALLPVPRFAPR
                     LLLGDMAEELLFYSAKAAPAAALATGYVFRFPALEPALRHVLRRPSAS"
BASE COUNT          260 a          674 c          681 g          228 t
ORIGIN      
        1 cgcctcggcc gcgccgcccc cgccctgctg gcacgaggcc gcctggaacg cgccgaaggc
       61 gccaccaacc tgctcgccga ccagctccgc gcccttccga tggaggtcgc gctccgctcc
      121 cgcgacttcc ggtagcagcg atcaggaatg atcagcgtca aagaaggccg ccgaacaggg
      181 cgctggggcg cagggtgagg cgcaggagcg cccgatacag cggattgtgg gccttctggg
      241 tgagggacag gccgcggcgg gcccactcca gggtggggag caggcggagc gcctcgcgga
      301 agtgctcggc ggcgccatcg tagtcaccgg tatagagcag cagccagccc tggttggcat
      361 gggtgcgtgg gttctccggg tcacgggcca gcaggccggc gagcaccgtc gcggcctcgg
      421 cccgtcgccc gaggcggatc agggcgaagg cacgcaggtt ggcgcagtca cggtggtccg
      481 gattggcgtc cagaccctgc gcagcggtcg cggcggcctc ggcccagcga cgcttggcga
      541 gctgaatctg tgccagcgcg gcgagtacgt cggcgttgcc cggcgccagg acaatcgcct
      601 tgcgggcggc agcctcggcc tcgtcgaacc gtccccggcg gcgcagcacg cccgcgagga
      661 tgtagtggcc gagcgctcca tccgggtcca ggccgaccgc ctcggccgcc tcgcgttcgg
      721 cgtcggcgag atcgccctga cgggccaggc agagggcgag cagcgcgtgt gtcccgccat
      781 gcccggcgcc gtggccgagc acgccgcgca gctcccgctc cgcaagctcg aactgggatc
      841 gcgcgaccag ccgccgcgcc cgctcgagcc ggtcagcgtg gaacccgtcg ccgggatcat
      901 tgccgagcat gagcgctcct cgcacgtttg ttgctcggtc gaatatacga caccggccga
      961 ccaggacctc catggcatgt gggaaattca ggaccccggg cgcagatacg cacgcgtcgt
     1021 gcgggttgac cgttcagggt cgctagctgg ctgacgggcg ccgcaggacg tgacggaggg
     1081 cgggctccag ggcggggaag cggaacacgt agcctgttgc gagcgcggcg gccggcgccg
     1141 ccttggcgct gtagaagagc agctcctccg ccatgtcacc gaggagcagg cggggagcga
     1201 accggggaac cggcagcaga gccgggcggc caagcacccg cgccagcgtg gcgctgtgct
     1261 cggcgttggt gaccgggtgc ggcgcggtcg cgtcgaccgg gccttccagc tcctcggtgg
     1321 cgatagcgtg gcggtagacg ccgaccacgt cgtccaaggc gatccagctc caccactgcc
     1381 gcccggagcc aagccggcca ccgaggccca gcttgtagag cggggccagg acggcgagca
     1441 tcccgccgcg agcctgcacc gggccggtgc ggacgtggac gacccggatt cccgccgccc
     1501 tggcgggatc cgccgccgcc tcccacgcct ggcagacacc ggacaggaag ccctggccgg
     1561 gcgaactccc ctcgctgagc gactcgtcgc cgcggtcgcc gtagtaatgg gcccccgagg
     1621 cgaccaccag tacccggggc cccgtgctcc cgccgctctc gcgggcgagg ccagccaagg
     1681 tctcggccag cagccgggtg ccgcgcaccc ggctctcggt gatctcccgc ttcacccccg
     1741 cggtccaggg gcgcgcgccg atccccttgc cggccaggtt gacgaccgcg tcgaccccgg
     1801 ccagcgcggc ggcgtcgatc atcccgtcgc gggggctcca gcg
//
LOCUS       DASWDC010000116         6850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_29134, whole genome shotgun sequence.
ACCESSION   DASWDC010000116 DASWDC010000000
VERSION     DASWDC010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29134"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VF995_03645"
     CDS             complement(<1..192)
                     /locus_tag="VF995_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342695.1"
                     /translation="MAKTRDALSVQQGPTRKGLQVRRLFTTKDRHPYDELSWEWRDAV
                     ITNWRDGTTAFEQRGLEFPS"
     gene            448..954
                     /gene="nrdR"
                     /locus_tag="VF995_03650"
     CDS             448..954
                     /gene="nrdR"
                     /locus_tag="VF995_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX9342696.1"
                     /translation="MRCPICGATDSRVIDSRPSDDGTAIRRRRECTTCGRRFNTFERV
                     EEVPLLVLKRRPGIREPFDPGKLVAGLEKACKNRPISRQEIIGIASDIEETLRARRQR
                     EVESQEIGIEVLNALQELDPVAYMRYASVYKDFQDPADFERELDSLASLRKSTPPKPH
                     PAAAPGRS"
     gene            complement(1031..1309)
                     /locus_tag="VF995_03655"
     CDS             complement(1031..1309)
                     /locus_tag="VF995_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9342697.1"
                     /translation="MRIRLLITVLLAVMLLLLVPGLARARGNGSAHPPAASGTPYTVA
                     PGDTLWSIANQIAPGRDPRPVIDRLQAANHLYGSLQAGQTLTIPASSR"
     gene            1561..2202
                     /gene="lexA"
                     /locus_tag="VF995_03660"
                     /pseudo
     CDS             1561..2202
                     /gene="lexA"
                     /locus_tag="VF995_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
     gene            complement(2250..2558)
                     /locus_tag="VF995_03665"
     CDS             complement(2250..2558)
                     /locus_tag="VF995_03665"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX9342698.1"
                     /translation="MATGEEVPGGVVKVIELVGSSPRSFSDAVRNAVQTAAQTIRNIR
                     GVEVISSSADVDGTGLITNYKVDCKIAFLIEATATASTGTLADTGEAVTTTATVSPPA
                     "
     gene            complement(2685..4085)
                     /gene="hflX"
                     /locus_tag="VF995_03670"
     CDS             complement(2685..4085)
                     /gene="hflX"
                     /locus_tag="VF995_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX9342699.1"
                     /translation="MTIMTDNTQNQPAPNQQTPTTPRRDDSDIHLVELRKLRLERVIL
                     VGVAIGGGTTHSAERSLEELRRLAETAGAEVLDGMLQRRARPDAGTFVGKGKTRELTE
                     RVRQLGADTVIMDDELSPSQLRKLEELVDAKVIDRTALILDIFAQHATSREGKAQVEL
                     AQLNYLLPRLRGWGESLSRHGGGIGPMRGPGETKIEIDRRRIRRRLTRLRRDLVQMGR
                     TRDIKRQGRERAGVPAVALAGYTNAGKSTLLNRLSGAGVLVEDRLFSTLDPTTRRLNL
                     PDGRSATLTDTVGFIAKLPHDLVEAFKSTLEEVGRADLLVHLVDAAQPDPGQQIEAVR
                     AVLAEVGAAEVPELLVLNKADLADDLTRARLARQFPDTPLVSATTGEGVERLLAEVAA
                     RLPHPVVCLTALLPYSRGDLLDRVYREGEVLEAEHVEEGTRLTLRAPAALAHALEPYG
                     TVVPTTTTSTTSTVSG"
     gene            complement(4184..4960)
                     /locus_tag="VF995_03675"
     CDS             complement(4184..4960)
                     /locus_tag="VF995_03675"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX9342700.1"
                     /translation="MFSGGAPLDAVVVERAVALLDAGVVRVAEPGEDGTWVTREWLKQ
                     AILLYFRARRMERMEVGPFEYYDKIPLKRGFEQLGVRVVPPATARYGSYLSRGVVLMP
                     SYVNIGAWVGPGTMVDTWATVGSCAQIGAGVHLAGGVGIGGVLEPVQARPVIVEDGAF
                     IGSRCIIVEGVRVGEQAVLGANVVVTASTPVIDVTVPEPVVHKGEIPPRAVVVPGTRP
                     RRFPAGEYHLATGLIIGWRDASADQRVSLNQVLREFDIAG"
     gene            complement(5083..6234)
                     /locus_tag="VF995_03680"
     CDS             complement(5083..6234)
                     /locus_tag="VF995_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9342701.1"
                     /translation="MRISPKLTQLGSYPFVQLDQAKDRMRARGVPLIDFGVGDPREET
                     PAFIRRALAGAITPRSSYPRAIGLPELREAIAAWVAGRFGVPLDPATEIIPTLGSKEV
                     IFSLAQVVIDPGGGKDLVLTTTPAYDVPRRGALMAGAEVAELPLQERNGFLPDLDAVT
                     DATWQRAALVWVNYPNNPTAAVAPLGFYQRLAELARRHDYLLASDEAYSELWYDQPPA
                     SALQVADRSNVVAVNTLSKRSCMTGYRSGFVAGDPRLVRALATYRPSTGVTPQEFVQR
                     ASIAAWGDEAHVETLRHRYAAKRRVLLDVLDAQGLRTVGSAATFYLWVAAPDGETSLG
                     FAQRLLEDGGIIVTPGSFLGAAGEGYVRVALVPTLEECERAATLLAKLL"
     gene            complement(6231..>6850)
                     /gene="dapF"
                     /locus_tag="VF995_03685"
     CDS             complement(6231..>6850)
                     /gene="dapF"
                     /locus_tag="VF995_03685"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX9342702.1"
                     /translation="RGYVTGAFALATRAGVKQLELHVDGRGCVDQVTVDMGAPGPARE
                     QPLALDGLLGPGWDGTGTAATLVSLGNPHAVVFVDGLVAGLDQLPVETVGPRIEHDRA
                     VFPAGGNVEFVQVLDAGAVRVRTWERGVGETLACGTGACAVAVAGQLRGVTGERLTVR
                     LRGGDLRLDWAPGRTVRMTGSAREVAHGTLGADLLARLGEGSLPA"
BASE COUNT          979 a         2357 c         2506 g         1008 t
ORIGIN      
        1 gctggggaac tcgaggccgc gctgttcgaa ggcggtggtg ccgtcgcgcc agttggtgat
       61 gacggcgtcg cgccactccc aggacagctc gtcgtagggg tggcggtcct tggtggtgaa
      121 caggcggcgg acctgaagac ccttgcgggt cgggccctgt tggacggaca gcgcatcccg
      181 cgtcttggcc acgaatcaac ctccttgcgg cccccgcgtc gggccggcga acggttttca
      241 cgctcccgcg ggggaaggct gggcagggcc cacctgggcg ggaggggctg ttggtttgta
      301 ctcaagcctg cgaactacag ctctgtgatt aagcacccgc tgtggggttc aggtcaagac
      361 cctgagcaca acatcttgca tcttgaagcc gattctacca ctatatcttg tggtggccga
      421 ttcacttcgg cccagggagg gaaagccgtg cgttgcccca tctgtggcgc tactgacagc
      481 cgagtcatcg actcgcgccc gtccgatgac gggaccgcca tccggcgtcg gcgggagtgt
      541 acgacctgcg gccggcgctt caacaccttc gaacgcgtcg aagaggttcc cctgctggtg
      601 ctcaaacggc gcccggggat cagggagccg ttcgacccgg gcaagctcgt cgccggtctg
      661 gagaaggcct gtaagaaccg gccgatctcc aggcaggaga tcatcggtat cgccagcgac
      721 atcgaggaga cgctccgggc tcgccgccag cgcgaggtgg agagccagga gatcggcatc
      781 gaggtcctga atgccctgca ggaactggac ccggttgcct acatgcgcta tgccagtgtc
      841 tacaaggact tccaggaccc ggccgacttc gagcgggagc tcgacagcct ggcgtcgctg
      901 cgcaagtcca ccccgccaaa gccacatccg gcggcggcgc cgggccgttc ctgagacccc
      961 cgcaccgccc cgtgtggcgt ctggcggccg gcccgcgacc agccatcctc tggtcgcggg
     1021 ccggccatgg tcaccggctg gatgccggga tggtgagcgt ctggccagcc tgcagcgagc
     1081 cgtacaggtg attggcggcc tgcaggcggt cgatcacggg ccgggggtcg cgtcccgggg
     1141 cgatctggtt ggcgatcgac cagagggtgt ccccgggggc gacggtgtag ggagtcccgc
     1201 tggcggcggg cgggtgggcg gagccgttgc ccctggccct ggccaggccg gggacgagca
     1261 gcagcagcat gacggcgagc agcacggtga tcagcagtcg gatgcgcaac ggccgggccc
     1321 cccttgacgt gacggatcgg atgcggagtt ctgaacgtac gttcgacgga tcgcatcttc
     1381 cctcgaacat ccgttcgtgt caacttcatc cggcgatccc ggagcgaccc gacccgcgag
     1441 cggggtcgaa caaaggatcg ccgaacagat gtttgatttc cgccatcggc gctgctagag
     1501 tcgttcactg agcagcaggc ggcggtcacc cgccacaatc catccagcaa ctgggcatca
     1561 gggggtaggt cgatggcgga ggagctcacc accaaacagc ggcgcatcct ggaggtgatc
     1621 cgcgaagccg tcaacgaccg cggctatccc ccctcgatcc gcgagatctg cgagaaggtc
     1681 ggcctcacct ccacctccag cgtccactcc cagctcttag ccctggaaaa gaagggctac
     1741 atccgccgcg accccaccaa gccacgcgcg atcgaggtct acctcgagga cccggtcgcc
     1801 gccgcccccc gcccgctgcc cacctacgcc ccgctgctga gcggcccgat cgccgccggt
     1861 accggcgtcc tacccgacga gcgggttgag ggcatgctcc cgctgccacg cgagctggtg
     1921 ggccagggcg agctgttcgt cctgcgggtc cgtggcgagt cgatgatcga ggcgggcatc
     1981 ttcagcgacg actacgtggt cgtccgccgt cagcccaacg ccgacaacgg cgacatcgta
     2041 gccgccatcg tcccgaatct cgaggaagag gcgaccgtca agcgactgtc acgacgggac
     2101 gggcacgtgc agctcctgcc ggccaacccg gcgttccagc caatcgatgg cgaccgggcc
     2161 agcatcctcg gccgggtcgt ggcggtgctc cgccgcctct gacgcccgcg ccgcccgcgt
     2221 ccggtccgtg ggcccggagc ggcgcggcgt tacgccggcg gcgacaccgt cgccgtggtg
     2281 gtgaccgcct cgccggtgtc ggcgagggtg ccggtcgacg ccgtcgcggt ggcctcgatg
     2341 agaaacgcga tcttgcagtc gaccttgtag ttggtgatca gcccggtgcc gtccacgtcg
     2401 gcgctgctgg agatcacctc gaccccgcgg atgttgcgga tcgtctgcgc ggcggtctgg
     2461 accgcgttgc ggacggcgtc gctgaacgac ctcggtgagc tgccgaccag ctcgatcacc
     2521 ttgacgaccc cgccgggaac ctcttcgccc gtagccatcg gctacctcca aaccgtgggt
     2581 gtggatccat ggtggctgtg gattccttgc ctatcccaat gagccggcct gccaaaccgg
     2641 caggattcac gggcagccag atccgatcca gctggagggg aacctcagcc gctcaccgtc
     2701 gaggtggtcg aggtggtcgt ggtggggacc acggtcccgt agggctccag ggcatgggcg
     2761 agcgcggccg gtgcccgcag cgtgaggcgg gtaccctcct cgacgtgctc ggcttcgagg
     2821 acctcgccct cccggtagac gcggtcgagc aggtcgccac ggctgtaggg gagcagcgcg
     2881 gtgaggcaca ccaccgggtg cggcaggcgg gccgcgacct cggcgagcag gcgctccacg
     2941 ccctcaccgg tggtggccga gaccagcggc gtgtcgggga actggcgggc cagccgggcc
     3001 cgggtcagat cgtcggccag gtcggccttg ttcagcacca ggagctccgg cacctcggcg
     3061 gcgccgacct cggcgagcac cgcccgcacg gcctcgatct gctggcccgg gtcgggctgg
     3121 gcggcgtcga ccaggtggac gagcaggtcg gcgcgcccga cctcctccag ggtggacttg
     3181 aacgcctcca ccaggtcgtg cggcagcttg gcgatgaacc cgaccgtgtc ggtcagcgtc
     3241 gccgagcgcc cgtcgggcag attcagccgc ctggtggtcg ggtcgagggt cgagaagagc
     3301 ctgtcctcga ccagcacccc cgcgccggag agccggttga gcagggtcga cttgcccgcg
     3361 ttggtgtagc cggccagggc gaccgccggg acgccggcgc gctcgcggcc ctgccgcttg
     3421 atgtcgcggg tgcggcccat ctggaccaag tcgcggcgca gtctggtgag gcgccggcgg
     3481 atgcggcgcc ggtcgatctc gatcttggtc tcgccggggc cgcgcatcgg gccgatgccg
     3541 ccgccgtggc gtgacaggct ctcgccccag ccgcgcagcc ggggcagcag gtagttgagc
     3601 tgggccagct cgacctgggc cttgccctca cggctggtgg cgtgctgggc gaagatgtcg
     3661 aggatcagcg cggtccggtc gatcaccttg gcgtcgacca gctcctccag tttgcggagc
     3721 tggctgggcg acagctcgtc atccatgatc acggtgtccg cgcccagctg gcggacccgc
     3781 tcggtcagct cccgcgtctt gcccttgccg acgaaggtgc cagcgtcggg gcgagcgcgg
     3841 cgttgcagca tcccgtcgag gacttcggcg ccggccgtct cggcgaggcg gcggagctcc
     3901 tccagggagc gctcggcgct gtgggtggtg ccgccgccga tggcgacgcc gaccaggatc
     3961 acccgctcga ggcggagctt gcgcagctcg acgaggtgga tgtcgctgtc gtcacggcgg
     4021 ggagtcgtgg gggtctgctg gttgggtgcc ggctggttct gggtgttgtc cgtcatgatc
     4081 gtcataccgt acaggctcaa ccgagcgtgg tcgcccggat attccacaga accatggccc
     4141 agggcgggcg ccccggcgag gcgggtcctc gcggccatgg ccctcaacct gcgatgtcga
     4201 actcccgcag gacctggttc agggagaccc tctggtcggc cgaggcgtcg cgccagccga
     4261 tgatcaggcc ggtggccagg tggtactcgc cagccgggaa gcgccttggc cgggtgccgg
     4321 ggacgaccac ggcgcggggc gggatctcgc ccttgtggac caccggctcg gggacggtga
     4381 cgtcgatcac cggggtcgag gcggtgacca ccacattggc gccgaggacc gcctgctcgc
     4441 cgacccggac gccctccacg atgatgcagc gcgagccgat gaaggcgccg tcctcgacga
     4501 tcaccgggcg tgcctggacc ggttcgagca ccccgccgat gccgaccccg ccggccaggt
     4561 ggactcccgc gccgatctgg gcgcaggagc cgaccgtcgc ccaggtgtcg accatggtgc
     4621 cggggccgac ccaggcgccg atgttcacat agctcggcat cagcaccacg ccgcgtgaca
     4681 ggtagctgcc gtagcgggcg gtcgcgggcg gcaccacccg cacgccgagc tgctcgaacc
     4741 cgcgcttgag cgggatcttg tcgtagtact cgaaggggcc gacctccatc cgctccatcc
     4801 gccgggcacg gaagtacagc aggatcgcct gcttgagcca ctcacgtgtc acccaggtgc
     4861 cgtcctcgcc cggctccgcg acgcggacca cacccgcgtc gagcagggcg acggcccgct
     4921 cgaccaccac ggcgtcgagc ggcgcgccgc cgctgaacat cgccgtgacc tgcccctcca
     4981 gttgggacag ctcggcatcg tccctggcgt cacggtcctg ggtcagcatc ggtggttggc
     5041 tcctcgtggg tgaattgttg gcgttcgtct gccagcagcg ttctacagca gcttggcgag
     5101 cagcgtcgcg gcgcgctcgc actcctccag cgtcgggacg agcgcgacgc gtacgtaccc
     5161 ctcgccggcc gcgcccagga acgacccggg cgtcacgatg atcccgccgt cctccagcag
     5221 gcgctgcgcg aacccgagcg aggtctcccc gtctggggcc gcgacccaga ggtagaaggt
     5281 ggcggcggag ccgacggtgc gcaggccctg ggcgtcgagc acgtcgagca gcacccgacg
     5341 tttggcggcg tagcggtggc ggagcgtctc gacgtgcgcc tcgtcgcccc aggcggcgat
     5401 cgaggcgcgc tggacgaact cctgcggcgt cactccggtc gagggacggt aggttgccag
     5461 cgcgcgcacc agccgggggt cgccggcgac gaagccggag cggtacccgg tcatgcagga
     5521 gcgtttggag agggtgttga ccgcgacgac gttgcttcgg tcggcgacct gcagcgccga
     5581 agccggcggc tggtcgtacc acagctcgct gtaggcctcg tcggaggcga gcaggtagtc
     5641 gtggcggcgg gccagctcgg cgaggcgctg gtagaacccc agcggggcga ccgcggcagt
     5701 cgggttgttc ggatagttca cccacaccag cgcggcgcgc tgccaggtcg cgtcggtgac
     5761 cgcgtcgagg tctggcagga agccgttgcg ttcctggagc ggcagctccg ccacctcggc
     5821 gccggccatg agcgcgcccc ggcgggggac gtcgtaggcg ggcgtggtgg tgagcacgag
     5881 gtctttgccg ccgccggggt cgatgaccac ctgggcgagg ctgaagatga cctccttgct
     5941 gcccagcgtc gggatgatct cggtggccgg gtccagcggc acgccgaagc gtccggcgac
     6001 ccaggcggcg atcgcctccc gcagctccgg caggccgatg gcgcgcgggt aactggaacg
     6061 gggggtgatc gcgccggcga gtgcccggcg gatgaacgcg ggcgtctcct cgcgggggtc
     6121 gccgacgccg aagtcgatca gcgggacgcc gcgggcgcgc atccggtcct tggcctggtc
     6181 gagctgcacg aacgggtagc tgccaagctg cgtgagcttc ggggagatcc tcatgctggc
     6241 aagcttccct ctccgagacg ggccagcagg tcggcgccca gagtgccgtg cgcgacctcg
     6301 cgagcggagc cggtcatgcg gacggtgcgg ccgggcgccc agtcgagccg caggtcgccg
     6361 ccgcgcaggc ggacagtgag gcgctcgccg gtcacgccgc gcaactgccc ggcgaccgcc
     6421 acggcgcagg cgccggtgcc gcaggcgagc gtctcgccga cgccgcgctc ccaggtgcgg
     6481 acccgcacgg cccccgcgtc gaggacctgg acgaactcga cgttgccgcc ggcggggaag
     6541 accgcccggt cgtgctcgat gcgtggcccg acggtctcga cggggagctg gtcgaggccg
     6601 gcgaccaggc cgtcgacgaa caccaccgcg tgggggttgc cgagcgacac cagggtcgcg
     6661 gcagtcccgg tcccgtccca gccgggcccg agcagcccgt cgagggcgag cggctgctcg
     6721 cgggcggggc cgggggcgcc catgtcgacg gtgacctggt cgacgcagcc gcggccgtcg
     6781 acgtgcagct ccagctgctt gacgccggcg cgggtcgcca gcgcgaaggc gccggtcacg
     6841 tagccgcggt
//
LOCUS       DASWDC010000117         9220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_29333, whole genome shotgun sequence.
ACCESSION   DASWDC010000117 DASWDC010000000
VERSION     DASWDC010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9220
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29333"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="VF995_03690"
     CDS             <1..1879
                     /locus_tag="VF995_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9342703.1"
                     /translation="YMAPEQAFAGTVDARSDLYATGIVLFELLCGQVPFDGDTPVAIL
                     IQQVRADLPPPRSLNPDLPVEVERLLVKALAKDPDQRYQSAAAMSEAISAVLAELSPR
                     VQARARVAAPAPASTSPSVVSGAGGSSPGGTAGGGTEELSAAYAAGVAAYSAGRWAEA
                     VTHLQRVATSDPGFEDVEILLETAKTKTAKAASGGAAVPEALLAPAPAAAASEAVPEP
                     RPDHAPGARNGSTPAPPAPSPAGRAGVPPVARRRPAAPAGPSAATPVSVPVPATARPR
                     AGGLDSEPGPGRRAGLRRWPLLVMLAVVAAVGIGLAFAAFGKPQPKGSTATSGRSGAP
                     AANPPWAALPPAPAVEESGGVAGFAGKVWVAGGLDATRQPLATVQVYDPALRTWSQGP
                     ALPKPVNHVALVSTGKQLLVIGGYANAGGGPVATVRRLDPVAGTWLDGPSLPAPRGAG
                     AAAWDGHRIVFAGGVGPDGRPSTDVFALQNGSWQQIGTLPAAREHLAAASDGRGTTFF
                     LGGEVNHPGGTKTVFNEVDVVHGSSIQQLRTLPTPRSSVAAFWSPASGACVVGGRGAD
                     QLTLSTVECVDAAGTMRNLASLATARHGLGAAVVNGTAYAVLGETANQRLKVGESLLL
                     R"
     gene            complement(1882..3264)
                     /locus_tag="VF995_03695"
     CDS             complement(1882..3264)
                     /locus_tag="VF995_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15"
                     /protein_id="HEX9342704.1"
                     /translation="MNPRLLLAIGVPVVLVAALLGSTRTSAPPPLFSSGLLGNATTLL
                     TISLEAGQTQDAAYVPGSTVLRLADGRLRYLPAGAIEAVTVPAGDPGAAAQIAAERAW
                     LALGWVPGASAAERDAASRSLLFLRLLQRPGGGVVAAATPYWAEVWPRDASFAAAAFG
                     RTGHQQEAGAVLDYLARIQRPDGSWPARAHPDGTPVSDGRQPELDADGWVCWATWLVS
                     GQGHDRALAALHWPMVRAAADLAASSLRANGLPPASPDYWEHHERQPTLGTAAALLTG
                     LRAAADLAAALGHDQDLRRWAAAAERLAAATREAFASPSGTAYHRYPGPASGQTFGLA
                     VAGGPDAAVTWLAPPFVPPDPRVRRAVARAATQLAPSGAGAVPGLAWTDPWTPATASF
                     ALAETALGDRAAAARQLGWLLAHRTSLGAFPERVRRADGAPRSVAPLAWTHAIVLLTL
                     AEAQHPLPIP"
     gene            complement(3285..4604)
                     /locus_tag="VF995_03700"
     CDS             complement(3285..4604)
                     /locus_tag="VF995_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342705.1"
                     /translation="MVVALLAGSGTALVVAKTTGTHGRAAAPATTPATAASATTATTA
                     PPATAAGPLAAQVRQVEAAVERLRQLRFTRSVPVTVESPQKMAAQLLKEINAGTDDAQ
                     LQRLDRALELLGELPPHTDLPKLLRAVQTESVLGFYVPGKPPGKGRLYVRSNNGLDPY
                     ARFVLSHELTHAVTDQHYDLTHSDRLNAPGKEDQSAAYTALLEGDATVMMGRYHDEVL
                     TAAEQADADRTGLTQATPELDAAPAALRESLLFPYEAGVAFVRALYAQGGWAAVDRAY
                     RDPPTSTEEILHPEKYLGTRDQPKPVQVPDLSGSLGSGWRTGTVAGWGEFDVRLMLEG
                     ALDVTTAERAAAGWGGGAVRSFQNGSRTALALRTVWDSPAEATEYCGLVTNWATSRFG
                     APVAGNRRWSGSGQQTALRCQGSTATWLSAPDGSSLDRLVTGLGGSS"
     gene            complement(4709..5419)
                     /gene="prcA"
                     /locus_tag="VF995_03705"
     CDS             complement(4709..5419)
                     /gene="prcA"
                     /locus_tag="VF995_03705"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEX9342706.1"
                     /translation="MSIGFGYVPPEQIIKDKADYARKGIARGRPMVVLEYDGGVLFVT
                     DNPSTHLHKVAELYDHIAFAAVGRFSEFENLRVAGITLADIRGYQYGREDVTSRSIAN
                     AYSQELGRIFMEGSKPYEVELCVAEVGSGDTPNALYHIGFEGTVNDHKRFLAMGGQAD
                     ELNAELQRNYGEGIDLDQALRLAVSTLSRAVQSGDGGPASGRLEVAVLERDRPRRAFR
                     RLSGDDLNARLKTGDGSG"
     gene            complement(5423..6223)
                     /gene="prcB"
                     /locus_tag="VF995_03710"
     CDS             complement(5423..6223)
                     /gene="prcB"
                     /locus_tag="VF995_03710"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEX9342707.1"
                     /translation="MRDPVGGLARFSPSFIDALREHAPELLPQPGSVAAGQQMPEVPH
                     GTTVAAIRYADGVIVGGDRRSTEGYAIAQRDVEKVFPADEYSAVAIAGAAGPAVEMVR
                     LFQTELEHYEKVEGLPLSLEGKANKLGQMVRGNLGMAMQGLVVVPLFCGYDQRRNLGR
                     IFRYDVTGGRWEDAEYHANGSGGKDARATIKKRYRHGMDREIALDTLVEGLYDAADED
                     AATGGPDLVRGIFPVVATVTAGGYQRLNDDDVRKAAERVVAARARGEG"
     gene            complement(6226..6441)
                     /locus_tag="VF995_03715"
     CDS             complement(6226..6441)
                     /locus_tag="VF995_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEX9342708.1"
                     /translation="MASGSGGGAEQKRRATGKQDAEQEDVQASADVTEKGEALKDELD
                     SLLDEIDDVLEENAEEFVKQYIQKGGQ"
     gene            complement(6532..8016)
                     /gene="dop"
                     /locus_tag="VF995_03720"
     CDS             complement(6532..8016)
                     /gene="dop"
                     /locus_tag="VF995_03720"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEX9342709.1"
                     /translation="MAITKVMGIETEYGITVPGSPDSNPVLASSLLVNAFSGRGRKVR
                     WDYEEESPLRDARGFEAPREPEVPSDDDLGLANVILTNGARYYVDHAHPEYSSPECLD
                     PRTLVVYDKAGERILAESAVRATQLTPGGRRIQVYKNNTDGKGTSYGTHENFLVDRTT
                     PFTRLVRDLTPFLCSRQIFAGAGSLVRDPADNSVAYQIAQRSQFFEVEVGLETTLKRP
                     IINTRDEPHADPERYRRLHVIIGDANMNEVATFLKTGSLAILLNLIEDDAVPSDDWQL
                     ANPVQALRAIAKDPTLRATVERKDGRMVTACEIQWGYLDAAKKYLKDRDIQHWQVEVL
                     ERWEAVLTQLEDDPFKAHRTVDWVTKYQLMRQIAERDSLELTDPKLALVDLQYHDVRL
                     DRGLYYRLAASGRVERLVAEQEIARAVADPPEDTRAFFRGRCLAKYASQIAAAGWDAI
                     IFDTGRETLQRVPMLEPTRGTAAHTAAILDASPAAEDLIRNLQG"
     gene            complement(8131..>9220)
                     /gene="arc"
                     /locus_tag="VF995_03725"
     CDS             complement(8131..>9220)
                     /gene="arc"
                     /locus_tag="VF995_03725"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEX9342710.1"
                     /translation="EVPDIAYQDIGGLSSQIEQIRDAVELPFLHADLFAEHQLKPPKG
                     ILLYGPPGCGKTLIAKAVANSLAKQVAEMTGRPSGRSYFLNIKGPELLNKYVGETERQ
                     IRLIFQRAKEKSNEGVPVIVFFDEMDSIFRTRGSGVSSDVENTIVPQLLSEIDGVEQL
                     KNVIVIGASNREDMIDPAILRPGRLDVKIKIERPNKDAAGDIFSKYLLATLPLATDVL
                     ERADGDREAALQRMIRETVERMYSDADENRFLEVTYANGDKEVLYFKDFNSGAMIENI
                     VSRAKKMAIKRFLKTGEKGIKTEDLNAAVTDEFKENEDLPNTTNPDDWARISGKKGER
                     IVYVRTLVGGSSKDFGRAIEQVNTGQYL"
BASE COUNT         1184 a         3164 c         3436 g         1436 t
ORIGIN      
        1 ctacatggcg ccggagcagg ccttcgccgg gaccgtggac gcccgctctg acctgtacgc
       61 caccggcatc gtgctattcg agctgctgtg cggccaggtg ccgttcgacg gcgacacccc
      121 ggtcgccatc ctcatccagc aggtccgcgc ggacctgccg ccgccgcggt cgctcaaccc
      181 cgacctgccg gtggaggttg agcgcctact ggtgaaggcg ctcgccaagg accctgacca
      241 gcgctatcag agcgccgccg cgatgagcga ggcgatcagc gcggtgctcg ccgagctgtc
      301 acccagggtc caggcccggg cccgggtggc cgctccggct ccggcctcga cgtcgcccag
      361 cgtggtgtct ggagctggag ggtcgtcgcc tggtgggaca gccggagggg gaaccgagga
      421 gctgtcagcc gcctacgcgg ccggcgtcgc cgcctacagt gccggccggt gggcggaggc
      481 ggtcacccac ctgcagcggg ttgccacctc cgatcctggc ttcgaagatg tggaaatcct
      541 gcttgagacc gccaagacca agaccgccaa ggcggccagt ggcggcgctg cggtccccga
      601 ggcgctcctc gcgccggccc cggcggcagc agcctccgag gcggtccctg agccccgtcc
      661 tgatcatgcg ccgggagcgc gcaatggttc cacacccgct cccccggccc cgtccccggc
      721 gggcagggcc ggggttccgc cggtggcacg gcgccggcct gccgcgcctg ccgggccttc
      781 tgcggcgacc cctgtgtcgg tcccggtccc agccacggct cggcctcggg ccggtggtct
      841 tgacagcgag ccgggcccgg gcaggcgtgc cggattgcgg cgctggccgc tgctggtcat
      901 gctcgcggtc gtcgcggcgg tggggatcgg gctggccttc gccgccttcg gcaagccgca
      961 gccgaagggg tcgacggcta catccgggcg ctcgggggca ccagcggcca acccgccgtg
     1021 ggccgcgctg ccaccggcgc cggccgtgga ggagtcgggc ggtgtcgccg ggttcgccgg
     1081 caaggtctgg gtcgcgggcg gcctcgacgc cacccggcag ccgctggcca ccgtgcaggt
     1141 ctacgacccg gccctccgga cgtggagcca ggggccggcg ctgcccaagc cggtcaacca
     1201 cgtcgcgctg gtgtccaccg gcaagcagct gctggtgatc ggcggctacg ccaatgccgg
     1261 tggcggccca gtggcgaccg tccgccggct cgacccggtg gcgggaacct ggctcgacgg
     1321 gccgagcctg ccagcacccc gtggcgcggg cgccgcggcg tgggacggcc accggatcgt
     1381 gttcgccggt ggcgtcggcc cggacggcag gccgtccacg gacgtcttcg ccctgcagaa
     1441 cggctcgtgg cagcagatcg gcaccctgcc ggcggcgcgg gagcacctgg ccgccgctag
     1501 cgacgggcgg ggaaccacct tcttcctcgg cggcgaggtc aatcaccccg gcggcacgaa
     1561 gaccgtcttc aacgaggtcg acgtggtgca cggcagcagc atccagcagc tcaggacgct
     1621 gccgacgcca cgcagctccg tcgcggcgtt ctggtcgcct gccagcgggg cttgcgtggt
     1681 cggcggccgg ggcgcggacc agctgacgct ctccacagtg gagtgcgtcg acgccgccgg
     1741 taccatgcgg aacctcgcgt cgctggccac cgcacggcac ggccttggcg ccgcagtggt
     1801 caacggcacc gcctacgcgg tgctcggcga gaccgccaac cagcgtctca aggtcggcga
     1861 atccttgctg ctgcgctgac gctacgggat cggcaggggg tgctgggcct cggcgagggt
     1921 gagcaggacg atcgcgtggg tccaggccag cggggcgacc gatcggggag cgccgtcggc
     1981 acggcggacc cgttcgggga aggcgccgag gctggtgcgg tgggcaagca gccagccaag
     2041 ctgccgggcg gcagcggcac ggtcgcccag ggcggtctcg gccagggcga atgaggcggt
     2101 tgccggtgtc cacgggtcgg tccaggccag gcccgggacg gcgccagcgc cgctgggggc
     2161 gagctgcgtg gcggcgcggg cgacggcacg ccgcacgcgg ggatccgggg gtacgaacgg
     2221 cggggccagc caggtcactg cggcgtccgg gccaccagcg acggcaagac cgaaagtctg
     2281 gccagacgcc gggcccgggt agcggtggta ggcggtcccg ctggggctgg cgaacgcctc
     2341 ccgggtcgcg gcggcgagcc gttcggcggc agccgcccag cgccgcaggt cctggtcgtg
     2401 gccgagtgcg gcggccaggt cggcagcggc gcggagcccg gtgagcagcg cggcggcagt
     2461 gccgagggtg ggctgacgct cgtggtgctc ccagtagtcc gggctggcgg gtggcaggcc
     2521 gttggcgcgc agggagctgg cggccaggtc ggcggcggcg cggaccatcg gccagtgcag
     2581 cgcggccagg gcgcggtcat gtccctggcc actgaccagc caggtggccc agcagaccca
     2641 gccgtccgca tccagctccg gctggcggcc gtcgctgacc ggggtgccgt caggatgcgc
     2701 acgtgccggc cagctaccgt cgggccgctg gatgcgggcc aggtagtcga ggaccgcgcc
     2761 ggcctcctgc tgatggccgg tgcggccgaa ggcggcggcg gcaaagctcg cgtcgcgcgg
     2821 ccacacctcg gcccagtacg gcgtagccgc ggcgacgacc ccgccgccgg ggcgctgcag
     2881 caggcgcagg aacagcagcg accggctggc cgcgtcgcgc tcggcggcgc tggcgccggg
     2941 gacccagccg agcgcgagcc aggcacgctc agcggcgatc tgggccgccg cgcccgggtc
     3001 accggccggg acggtcaccg cttcgatagc tccggcgggg aggtagcgga gccgcccatc
     3061 ggcgagccgc agcaccgtac tgccgggcac gtaggccgcg tcctgggtct gccctgcctc
     3121 cagcgagatg gtcagcaggg tggtggcgtt gccgagcagc ccgctgctga acagcggcgg
     3181 cggcgcgctc gtcctggtcg acccgagcag ggccgccacc agcacgaccg gcacgccgat
     3241 cgccagcagg agccgaggat tcatccagcg ccggcctcgc cggctcagga ggacccgccg
     3301 agacccgtga cgagccggtc gaggctcgac ccatccgggg cggagagcca ggtcgccgtg
     3361 ctgccctggc agcggagcgc ggtctgctgg ccggaacccg accagcggcg gttgccggca
     3421 accggagcgc cgaaccggct ggtcgcccag ttggtgacca acccgcagta ctcggtggcc
     3481 tcggcaggcg agtcccacac cgtccgcagg gccagggcgg tccgtgaacc gttctggaag
     3541 ctgcgcaccg caccgccccc ccagccggca gcggcccgct ccgcggtggt gacgtcaagg
     3601 gcgccctcca gcatcaaccg cacgtcgaac tcgccccagc cggccaccgt gccggtccgc
     3661 cagccgctgc cgagcgaccc ggagaggtct ggcacctgga ccggcttcgg ctggtcgcgg
     3721 gtgcccaggt acttctcggg gtggaggatc tcctcggtgg aagtgggtgg gtcgcggtag
     3781 gcacggtcca ccgccgccca gccaccctgc gcgtacaggg cccgcacgaa ggcgactccg
     3841 gcctcatagg ggaacagcag cgactcccgc agggccgccg gggccgcatc cagctcgggc
     3901 gtcgcctgcg tgaggccggt ccggtcagcg tctgcctgct cagcggcggt gaggacctca
     3961 tcgtggtagc ggcccatcat cacggtcgcg tcgccctcaa gcagcgccgt gtaggcggcc
     4021 gactggtcct ccttgcccgg cgcgttgagg cggtcgctgt gggtcaggtc gtagtgctgg
     4081 tcggtgaccg cgtgggtcag ctcgtgggag agcacgaagc gggcgtaggg gtccaggccg
     4141 ttgttgctgc ggacgtacag acgccccttg ccgggaggct tgccggggac gtagaagccg
     4201 agcacgctct cggtctggac cgcccgcagc agcttcggca ggtcggtgtg gggcggcagc
     4261 tcgccgagca gctcaagggc ccggtccagg cgctgcagct gggcgtcgtc ggtgccggcg
     4321 ttgatctcct tgagcagctg cgccgccatc ttctgcggcg actcgacggt gaccggcacc
     4381 gaccgggtga agcggagctg gcgcagccgc tccacggccg cctccacctg gcggacctgg
     4441 gcggcgagcg gtcctgcggc ggtggccggc ggcgccgtgg tggccgtggt ggcgctggcc
     4501 gctgttgccg gggtggtggc gggtgccgca gcgcggccgt gggtgccggt ggtcttggcg
     4561 accaccagcg cggtgccgga gccagccagc agcgccacga ccacgatgag gacgagggcg
     4621 agcctgcgcc tgcctggtgc cggtccctgc ggcggcggta tcggggccgt tgggggcggg
     4681 tcaggcgggg ctggctcgcc ccaggcggtc atcccgaacc gtctcctgtc ttcagccggg
     4741 cgttcaggtc gtcgccggag aggcggcgga aggcacgtcg gggccggtcc cgctccagca
     4801 cggcgacctc cagccggccg ctggccgggc cgccgtcgcc gctctgcacg gccctggaga
     4861 gggtgctgac ggcgagccgt agggcctggt ccaggtcgat gccctcgccg tagttgcgtt
     4921 gcagctcggc gttcagctcg tcggcctggc cgcccatggc gaggaagcgc ttgtggtcgt
     4981 tgacggtgcc ctcaaagccg atgtggtaga gggcgttggg ggtgtcgccg ctgcccacct
     5041 cggcgacgca cagctcgacc tcgtagggtt tgctcccctc catgaagatg cggccgagct
     5101 cctgggagta ggcattggcg atcgaccgag aggtgacgtc ctcgcgcccg tattggtagc
     5161 cgcggatgtc ggctagggtg atgccggcca cgcgcaggtt ctcgaactcc gagaagcgcc
     5221 ccaccgccgc gaacgcgatg tggtcgtaca gctcggcgac cttgtggagg tgggtcgagg
     5281 ggttgtcggt gacgaacagg acgccgccgt cgtattcgag cacgaccatc ggccggccgc
     5341 gggcgatgcc tttacgcgca tagtcggcct tgtccttgat gatctgctca ggggggacat
     5401 acccgaaccc gatcgacacc gctcagccct ccccacgtgc gcgggcggcg accactcgct
     5461 cggccgcctt gcgcacgtcg tcgtcgttga ggcgctggta gcccccggcg gtcacggtcg
     5521 ccaccaccgg gaagatgccg cggaccaggt cgggcccgcc ggtggcggcg tcctcgtcgg
     5581 ccgcgtcata gaggccttcg accagcgtgt cgagcgcgat ctcgcggtcc atgccgtggc
     5641 ggtagcgctt cttgatggtg gcgcgggcgt ccttgccgcc ggagccgttg gcgtggtact
     5701 cggcgtcctc ccagcggccg ccggtgacgt cgtagcggaa gatgcggccc aggttgcggc
     5761 gctggtcgta gccgcagaac agcggcacca ccaccagccc ctgcatggcc atgccaaggt
     5821 tgccgcgcac catctgcccg agcttgttgg ccttgccctc cagcgacagg ggaagcccct
     5881 cgaccttctc gtagtgctcc agctcggtct ggaacagccg caccatctcg acggccggcc
     5941 ccgccgcccc ggcgatcgcc accgcggagt actcgtcggc cgggaagacc ttctcgacgt
     6001 cccgctgggc gatggcgtac ccctcggtcg aacgccggtc acccccgacg atcaccccgt
     6061 cggcataccg gatcgctgcc accgtggtcc cgtgcggcac ctcgggcatc tgctgcccgg
     6121 ccgcgacgct acccggctgc ggcagcagtt caggcgcatg ctcccgcagc gcatcgataa
     6181 acgatgggct gaaccgcgcc agcccgccga cggggtctct cacgcctatt gaccgccctt
     6241 ctgaatgtac tgtttgacga attcttccgc attttcctct aggacgtcat cgatctcatc
     6301 aaggagagaa tcgagttcgt ccttgagtgc ttctcccttt tcggtcacgt cggccgatgc
     6361 ctggacgtct tcttgctcgg cgtcctgctt gccggtcgcg cgccgcttct gttcggcgcc
     6421 gccgccgctg ccgctggcca tcgcgtccct cctccacgtg ctgatctggc gattctgcca
     6481 cggttgggcc accgcgtccg gacgacccgg ttctgccggt tgggttgtgg gctatccctg
     6541 caggttacgg atcaggtctt cggctgccgg gctggcgtcg aggatagcgg cggtgtgcgc
     6601 ggccgtgcct ctggtgggtt ccagcatcgg gacccgctgc agggtctcgc ggccggtatc
     6661 gaagatgatc gcgtcccagc cggccgcggc gatctgcgag gcgtacttgg ccaggcagcg
     6721 gccgcggaag aaggcgcggg tgtcctcggg cgggtccgcc accgcgcggg cgatctcctg
     6781 ctcggcgacc agccgctcga cccgcccgga ggcggcgagg cggtagtaca ggccgcggtc
     6841 caggcgcacg tcgtggtact gcaggtcgac gagggcgagc ttggggtcgg tcagctccag
     6901 gctgtcccgc tcggcgatct ggcgcatgag ctggtacttg gtgacccagt cgacggtgcg
     6961 atgggccttg aaggggtcgt cctccagctg ggtgaggacc gcctcccagc gttccagcac
     7021 ctcgacctgc cagtgctgga tgtcgcggtc cttaaggtac ttcttggccg cgtccaggta
     7081 gccccactgg atctcacagg cggtgaccat gcggccgtcc ttgcgctcga cggttgcccg
     7141 cagggtcggg tccttggcga tggcgcgcag cgcctgcacc gggttggcga gctgccagtc
     7201 gtcgctgggg acggcgtcgt cttcgatcag gttgaggagg atcgccagcg acccggtctt
     7261 gaggaaggtg gcgacctcgt tcatgttggc gtcgccgatg atcacgtgca ggcggcggta
     7321 gcgctcgggg tcggcgtgcg gctcgtcgcg ggtgttgatg atgggccgct tgagggtggt
     7381 ctccaagccg acctcgacct cgaagaactg ggagcgctgg gcgatctggt aggcgaccga
     7441 gttgtcggcg gggtcgcgca ccaggctgcc ggcgccggcg aagatctggc ggctgcacag
     7501 gaacggggtc aggtcgcgga ccagccgggt gaagggggtg gtccggtcga ccaggaagtt
     7561 ctcgtgggtg ccgtaggagg tgcccttgcc gtcggtgttg ttcttgtaga cctggatgcg
     7621 gcggccgccg ggggtgagct gggtggcgcg gaccgcggac tcggccagga tgcgctcccc
     7681 cgccttgtcg tagaccacca gggtgcgcgg gtcaaggcac tccggcgagg agtactcggg
     7741 atgggcgtgg tcgacgtagt agcgggcgcc gttggtgagg atgacgttgg ccaggccaag
     7801 gtcgtcatcg ctgggcactt cgggctcgcg cggtgcctcg aagccccgcg catcgcgcag
     7861 cggcgactcc tcctcgtagt cccagcggac cttgcggccg cggcctgaga atgcgttcac
     7921 aagcaggctc gatgccagga cggggttgga gtcgggtgag ccgggcacgg taatcccgta
     7981 ctcggtctcg atccccatga ccttggtgat ggccatccgt ccccctatcc ccggatgcgg
     8041 cgtggttggc cggacgcgac cggtgcgcca ccgggaggga acggggcggc ccaatggccg
     8101 ccccgcgcgt ccggctcgtc cgtctcgatg ctacaggtat tggccagtgt tgacttgttc
     8161 gatcgcacgc ccgaagtcct tggagctgcc gccgaccagg gtccggacgt agacgatgcg
     8221 ctcgcccttc ttgccggaga tgcgagccca gtcgtcgggg ttggtggtgt tgggcaggtc
     8281 ctcgttctcc ttgaactcgt cggtcaccgc cgcgttcagg tcctcggtct tgatgccctt
     8341 ctcgccggtc ttcaggaacc gcttgatggc catcttcttg gcgcgcgaca cgatgttctc
     8401 gatcatcgcg cccgagttga agtccttgaa gtacaggacc tccttgtcgc cgttggcgta
     8461 ggtgacctcg aggaagcggt tctcgtcggc gtcggagtac atccgctcga cggtctcgcg
     8521 gatcatccgc tgaagcgccg cctcgcggtc gccgtcggcc cgctctagga cgtcggtcgc
     8581 cagcggcagg gtcgccagca ggtacttgga gaagatgtcg ccggccgcgt ccttgttggg
     8641 ccgctcgatc ttgatcttca cgtccaggcg gccgggccgc aggatcgccg ggtcgatcat
     8701 gtcctcgcgg ttggaggcgc cgatcacgat cacgttcttg agctgctcca caccgtcgat
     8761 ctcgctgagc aactgcggga cgatggtgtt ctcgacgtcg gaggagacgc ccgagccgcg
     8821 ggtgcggaag atcgagtcca tctcgtcgaa gaacacgatc accggcacgc cctcgttgga
     8881 cttctccttg gcgcgctgga agatcagccg gatctgccgc tcagtctcgc cgacatactt
     8941 gttgagcagc tccgggccct tgatgttgag gaagtagctg cgcccgctgg gccggccggt
     9001 catctccgcc acctgcttgg ccagcgagtt ggcgaccgcc ttggcgatca gcgtcttgcc
     9061 gcagcccggc gggccgtaca gcaggatgcc cttgggcggc ttgagctggt gctcggcgaa
     9121 caggtcggcg tgcaggaacg gcagctccac cgcgtcgcgg atctgctcga tctgggagga
     9181 gagcccgccg atgtcctggt aggcgatgtc gggcacctcc
//
LOCUS       DASWDC010000118         1753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_432343, whole genome shotgun sequence.
ACCESSION   DASWDC010000118 DASWDC010000000
VERSION     DASWDC010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432343"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VF995_03730"
     CDS             complement(<1..473)
                     /locus_tag="VF995_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9342711.1"
                     /translation="MTALLEVSDLSKHFLKDEREIVALRDFTLSVEQGSFVTLLGRSG
                     CGKSTLLNLVAGLLSGTSGTVAYRGQQVDGPRRELGYLTQSDTLMPWRDVRRNVELPL
                     ELRGVDKRERRSVAHRLIDTVGLHGFERHYPRELSGGMRRRASLARMLCGDPETLL"
     gene            612..>1753
                     /locus_tag="VF995_03735"
     CDS             612..>1753
                     /locus_tag="VF995_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9342712.1"
                     /translation="MPGPRDAPSTAAVVADYLRAAGVRFVFAYPGESIIELMEAARGA
                     GVEVILGRREGTAAFMAEAAAMATGTPGVCLSTLGPGSTALVNGVAAATWDRVPMLAI
                     SGQIETSREQLFTHQVVDHKLLFSPVAKWAGRIEASAAGGIMRKALALAVAERPGAVH
                     LTGAADTFKAPARDAEVALPPLGAAVETVQVRYAPGSHPGGRADPVERLRRARRPVVL
                     AGIAATRCDASGALVRLAETAGVPVVVAPMSKGVFPEDHPYFAGVLDMACNQVLWDFL
                     AGSDLLVSAGFDPVELLRPWPLTMPVLHVDTTPNVDQIYFSDCEVVGDVAASLDWLTG
                     AWHGEPRWTEREVAAHRDRLRTAYYAGRVEGRLNPTDVVDVMRAATS"
BASE COUNT          228 a          640 c          629 g          256 t
ORIGIN      
        1 aggagggtct ccggatcacc gcagagcatg cgggcgaggc tggcacggcg gcgcatgccc
       61 ccggacagct cccgtgggta gtgccgctcg aagccgtgca gcccgacagt gtcgatcagg
      121 cggtgcgcca ccgaacgccg ctcccgcttg tcgaccccgc gcagctccag cggcagctcc
      181 acgttgcggc gcacgtcccg ccatggcatc agcgtgtcgc tctgggtgag gtagcccagc
      241 tcccgccggg ggccgtcgac ctgctggccg cggtaggcga ccgtcccgct ggtgcccgac
      301 agcaatccgg cgacgaggtt gagcagggtc gacttgccgc agccgctgcg gccgagcagc
      361 gtgacgaagc tgccctgctc cacgctgagg gtgaagtcgc gcagggccac gatctcccgc
      421 tcgtccttga ggaagtgctt ggagagatcg ctgacctcca gcagcgcggt catcgcgctc
      481 tggggtcccg cggattcgca gtcatttgga atgcattatg catgatttgt gggatgatag
      541 tagacattat tgtcaacaat ctgacccggg ctggcggcgc gatgccggcg gcacggtgga
      601 ggtgatggcg gatgcctggt ccgcgcgacg cgcccagcac ggccgcggtg gtggccgact
      661 acctccgcgc ggccggcgtc cggttcgtgt tcgcctatcc gggcgagtcg atcatcgagc
      721 tgatggaggc cgcccgcggc gccggtgtcg aggtgatcct cggccggcgg gagggcaccg
      781 ccgcgttcat ggccgaagcc gccgcgatgg cgacgggcac cccgggcgtg tgcctgtcga
      841 ccctcgggcc cggctcgacc gcgctcgtca acggcgtcgc agcggcaacc tgggaccggg
      901 tccccatgct ggcgatctcc ggccagatcg agaccagccg cgagcagctc ttcacccacc
      961 aggtcgtcga ccacaagctg ttgttctccc cggtcgccaa gtgggccggc cggatcgagg
     1021 ccagcgcggc cggcggcatc atgcgcaagg cgctggccct ggccgtcgcg gaacgtccgg
     1081 gcgccgtcca cctcaccggg gcggccgaca ccttcaaggc gccggcgcgg gacgccgagg
     1141 tcgccctccc gccgctgggc gccgccgtgg agaccgtcca ggtgcgctac gccccgggct
     1201 cccaccccgg cggccgcgcc gatccggtgg agcggctgcg gcgcgcgcgg cgcccggtgg
     1261 tgctcgccgg catcgcggcg acccgctgcg acgcctccgg ggcgctggtc cgcctggccg
     1321 agaccgcggg cgtccccgtg gtggtggcgc cgatgtcgaa gggcgtgttc ccggaggacc
     1381 atccgtactt cgccggcgtg ctcgacatgg cctgcaacca ggtgctgtgg gacttcctcg
     1441 ccggcagcga cctgctggtc agcgccgggt tcgacccggt cgagctgctt cggccctggc
     1501 cgctgaccat gccggtcctc cacgtcgaca ccacgccgaa cgtcgaccag atctacttca
     1561 gcgactgcga ggtggtcggc gacgtcgccg ccagcctcga ctggctcact ggcgcctggc
     1621 acggcgagcc ccgctggacc gagcgggagg tcgccgcgca ccgcgaccgc ctccgcacgg
     1681 cctactacgc cgggcgggtc gagggccgcc tcaatccgac cgacgtcgtc gacgtcatgc
     1741 gcgcggccac ctc
//
LOCUS       DASWDC010000119         3727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1281412, whole genome shotgun sequence.
ACCESSION   DASWDC010000119 DASWDC010000000
VERSION     DASWDC010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281412"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VF995_03740"
     CDS             <1..1464
                     /locus_tag="VF995_03740"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX9342713.1"
                     /translation="ARRLAPLSAVALVVLLVVLFTAGPVTANVALTLVSTDPFTNTTS
                     QHQTEVEPDTFVNGSTIVSAFQVGRFFDGGSSDIGFATSTNGGASWTNGFLPGITKFQ
                     GGGTFDRTSDPAVAFDAAHNVWMISSLGITESGGVRAIAVLTSRSTDGGLTWSNPVTT
                     ATGTNLDKNWIVCDNTASSPFFGHCYTEFDDPNDSDRLKMSTSTNGGTAWGAALNTGD
                     NATGLGGQPVVRPNGTVIVPYANAGETQIRSFRSVDGGASWRSTVPVSAVTRHTVAGG
                     LRTGPLPSAEIDSAGTVYVVWQDARFVSGGTRNDIVMSKSTSETTWGPVTRVTSDGAD
                     HFIPGIGVDKSTAGATARVAITYYDYTTPSCMAPNCQLNVASVTSTNGGSTWSAPLQL
                     AGPMTLSWLANTNQGRMVGDYVSTSIPSGANAFSVFAVATAPTGTTFHENMDVPTGGV
                     PVTGGAIRAARTPRFRGAGGGGAPAAPGAARPPLLSR"
     gene            complement(1644..3566)
                     /locus_tag="VF995_03745"
                     /pseudo
     CDS             complement(1644..3566)
                     /locus_tag="VF995_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
BASE COUNT          518 a         1356 c         1336 g          517 t
ORIGIN      
        1 gcccgcaggc tcgcacccct ctccgccgtg gccctcgtcg tcctgctggt cgtgctgttc
       61 accgccggtc cggtgaccgc caacgtcgcg ctcaccctgg tgagcaccga tcccttcacc
      121 aacaccacca gccagcacca gaccgaggtc gagccggaca cgttcgtgaa cggctccacg
      181 atcgtgtcgg ccttccaggt gggccgcttc ttcgacggcg gctcctccga catcggcttc
      241 gccacctcga ccaacggagg cgccagctgg accaacgggt tcctgcccgg gatcaccaag
      301 ttccagggtg gcggcacctt cgaccgcacc agcgaccccg cggtcgcctt cgacgcggca
      361 cacaacgtct ggatgatctc ctcgctgggg atcactgaga gcggcggggt ccgcgcgatc
      421 gccgtgctca ccagccgctc caccgacggc ggcctcacct ggtccaaccc ggtgaccacc
      481 gccaccggca ccaacctcga caagaactgg atcgtctgcg acaacaccgc gtcgagcccg
      541 ttcttcggcc actgctacac cgaattcgac gaccccaacg acagcgaccg cctcaagatg
      601 agcacctcca ccaacggtgg gaccgcctgg ggcgcggcgc tcaacaccgg cgacaacgcg
      661 accggcctcg gcggccagcc ggtggtacgc cccaacggca ccgtgatcgt cccctacgcc
      721 aacgccggcg agacccagat ccgctccttc cgctcggtcg acggcggcgc gagctggcgc
      781 agcaccgtgc cggtgtcagc cgtgacccgc cacaccgtcg cgggcggcct gcgcaccggc
      841 ccgctgccgt cggccgagat cgacagtgcc ggcacggtct acgtggtctg gcaggacgcc
      901 cgcttcgtct ctggcggcac ccgcaacgac atcgtcatga gcaagtcgac cagcgagacc
      961 acctggggtc cggtcacccg cgtcaccagc gacggcgccg accacttcat ccccgggatc
     1021 ggggtcgaca agagcaccgc gggcgcgacc gcccgggtcg ccatcaccta ctacgactac
     1081 accaccccga gctgcatggc gcccaactgc cagctcaatg tcgcctcggt gacctcgacc
     1141 aacggcggca gcacctggtc cgcgccgctg cagctcgccg gcccgatgac gctgagctgg
     1201 ctggccaaca ccaaccaggg ccgcatggtc ggcgactacg tctccacctc gatcccgtca
     1261 ggcgccaacg ccttctcggt cttcgccgtg gcgaccgccc cgaccggcac caccttccac
     1321 gagaacatgg acgtcccgac cggcggcgtc ccggtcaccg gcggggcgat ccgcgccgct
     1381 aggacgcctc ggttccgagg agcgggaggc ggcggtgcgc cagctgctcc aggagccgct
     1441 cggcctccgc tcttgtcgcg gtgacgttgg cgagcttgac gtgctcgtag ccgcggatgc
     1501 ggtcgggcag gtcggcgagt gcggccacca ggtcgtggtt gtccgggcgc aggcgctcca
     1561 gcgcccgggc gaccacatcc tggtaccaga tcctggtacc agctgatcag ctcgcgttcg
     1621 gtgcggcgca acttggtgta gccgaacggg tcgaagggcg tgccgcggag gcggcgcagg
     1681 gcccgtagcg cccggaacgt ggggcgggcc gccgccgggc tgagcgacag cttgcggtcc
     1741 aggccgaggg cgcgcagcac cggcgggtgc agctggtagc gcacctgccg ggggttcgcg
     1801 aaggttgccc gcacgccggc gtcgagggcg ggctgcaggt acaggcgcgc gacctcgtac
     1861 tcgtccttgt aggcgagcag cttgtagagg ttgcggatga ccgcgtgggt gacctcgtgg
     1921 cgctccgggc ccacggcccg ctcccgcgcg tcggccgcca ggaccgcgtc caggtagcga
     1981 cccgcgtagg cggggctctg gtagttggcg aggtcggcaa gccgagtggc cagcagctcc
     2041 cgggaggtct cgtcgagcct ggcggtggcg gctcggtcgc gcagccgctc gaccgccgcc
     2101 cgccctccct tcgaaagacc ggcgagggcg atctgctccg gggtgccggc cgggttcggc
     2161 ggccgatcgg ccgcggcgcg gaccgcctcc tggtcggcga cccaggcgcg gccgtggcgg
     2221 aatgcctgga ggttggcctc gacgccgacg ccgttcatgg tgatcgcctg ctcgatgctg
     2281 gcggcgctga tcggcagccg ccccgcctgc cacgcggcgc cgaggacgat catgttggtc
     2341 atcatgaagt cgccgaacag cgactcggcc agctcgcggg cggggacggc gacggtccgg
     2401 tcggggcggg tgtggcgggc gagcatcgcc agcatgccgt ccacgtcggg gaccgcccag
     2461 acgtcgcgga ccatctcgcc ggtggggaac agggtcgagt tgaccaccgc ggcggtccgc
     2521 gccgggtcgg cccggtccag gttctccggc gagacggtgg cgaccaggtc caggccgagg
     2581 atcacgtcgg cctggccgag gccgaccttg gaggccggca gcggcgtgcc cgccggggcg
     2641 acgatcaggc tggaaagcac cggaccccac ttctgggccg cgccggtctg gtcgaagctg
     2701 gcaacctcca ggccgtccag ggtcgcggcc accgcgagga tcgcgttggc ggtgagcact
     2761 ccggtcccgc cgacgccggg cgcgtagagg cggtaggggc ccgccagcgc gacccgctcc
     2821 ggctccggca gcgcggccgg gtccagggcc ggggccgtgc ggcgggtgta gccggtgccg
     2881 ggggcgacgt ccacggtgac gaaggagggg cagtcgccct gcaggcaggt gtagtcctgg
     2941 ttgcaggtcg aggtgtggat ctgggtcttg gggccgaact cggtgtccac cttctgcagc
     3001 gacatgcagt tggagacccg gccgcagtcg ccgcagttct cgcagacccg ctcgttgatc
     3061 atgacgtagc ggtcggggcg ctccagcgtg ccgcgcttct gctggcggcg gcgctcgttg
     3121 gcgcacgact cgtcgtagag catcacggtc accccggtca gggcggccag ctcgcgctgg
     3181 acggcgacca cgtcccgggc ggggcgcacc tggaccgggg agggcaggcc aagtttcgtg
     3241 tagcggccgg gctccttggt gaccagggcg acgcgggcga cgccctccag cgccagcagc
     3301 cgcgccagct ccggtaccga gcgggcgcca ggggcggcct gggcgccggt gttggcgacc
     3361 gcgccgttgt agagcagctt gaaggtgatg ttcacgccgg ccgcgacaca ggcgcggatg
     3421 ttgtcgtagc tggagtgcca cagcgacccg tcgcccacgt tctggaacat gtgcggcttg
     3481 tcggtgaagg gcgccagccc gatccacggc aggccctcgc cgccgtactg ggtcatcgcc
     3541 tcgacgcgcc gctccggctg ctccatcacc gacgcgaagc tgtgacagcc cgggctgccc
     3601 caggcgatct ccccctcggg caggcgggtc gagtgggagt gggggcagcc cgagcagtag
     3661 ttcggcgtgc gccgcacgat cgcctggtag cgccggcccc ggacgccctc gatctggccc
     3721 aggcgcg
//
LOCUS       DASWDC010000120         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1446739, whole genome shotgun sequence.
ACCESSION   DASWDC010000120 DASWDC010000000
VERSION     DASWDC010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446739"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VF995_03750"
     CDS             <1..244
                     /locus_tag="VF995_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342714.1"
                     /translation="CSPEVATRALATGTYMAQELRRYWAFAATLALGTGIGPAHPPVA
                     EVAAGLARDLPATVIHLPNQPGPRLAELLEEAKTWT"
     gene            235..1683
                     /locus_tag="VF995_03755"
     CDS             235..1683
                     /locus_tag="VF995_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX9342715.1"
                     /translation="MDVTVLTTDHSEREPRPGARVFASGDAPANPAVLRLLGDKLAGA
                     DLATGPVVLPDFHHKSSMEMPSSIAVATRGTIRPTLTSSSVNCGMALLAFDAEVPSDA
                     AITRFYDKVLERFPHPATRRRDLSGHEVIRAAEEGGDFAAERWGVDPANLTRMEKDGR
                     IDLARHGGAARLRKELPWLVVQFARMRFGTIGPSNHFVELQRVEEVFDQRTAEAFGVR
                     EGQLTLQYHGGGGVLAGQLGRLFGRREKISRPVKAEMAVTKPLYHLASARSLAEFRLR
                     RALYFSDGCPPVDADGPEGARLLLAQAAAMNYGFAFRLATYASLQEFAAQVFGARDSH
                     LVVDSPHNSIYEEELDGERVILHRHNSCQAYPASRMAGHPVFGTTGQAILLPGTNKTS
                     SYLCAAEEGAGASLWSACHGAGTMIDDFVARGRSGPDPKGHTTLRFTYKGDAPAVVPH
                     LDDNGVNEALDILRVHGIARPVARLRPLAVLN"
     gene            1712..>2681
                     /locus_tag="VF995_03760"
     CDS             1712..>2681
                     /locus_tag="VF995_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX9342716.1"
                     /translation="MRRTVTDTTASTGRVVARNTGAQVVCFGARVIAGLLAVVLVARQ
                     GGPHELGVFQFGLTLSQMFGFAIGLGLPNLLTREIARDPEHSRDWVGLGVFVALSAGT
                     IVTALLTIGMRFSGRPHEMVVSVSLAAVALCFDTAGRLEFAAFEGWQRMWLEAGATCL
                     QEAAFIAGTITMLATGHGAAGVMVAYLLSRVLGAFAGWVIASWYLHGPVAPRPPLAFL
                     RRTLRVTVPFALDDAMSLTYIRIDAVLLGFIKGADAVGLYQAATNLVLYLNILARMLN
                     TALYPRMSKAWPDPARLGRWRDLSLRTLGLISLPAMVGSFLLAHQIF"
BASE COUNT          345 a         1051 c          907 g          378 t
ORIGIN      
        1 ctgcagcccc gaggtcgcca cccgggcact ggccaccggc acctacatgg cgcaggagct
       61 gcgccgctac tgggcgttcg ccgccaccct cgcgctcggc accgggatcg gcccggcgca
      121 cccgccggtc gccgaggtcg ccgctggcct cgcccgcgac ctgccggcga ccgtgataca
      181 tctcccgaac cagccggggc cccgcctcgc ggagctcctc gaggaggcca agacgtggac
      241 gtgacggtcc tgaccaccga ccacagcgag cgcgagccac ggcccggagc gcgggtcttc
      301 gcctccggcg acgcgcccgc caacccggcg gtgctgcggc tgctcggcga caagctcgcc
      361 ggcgccgacc tcgccaccgg cccggtggtg ctgcctgact tccaccacaa gtcgtcgatg
      421 gagatgccct ccagcatcgc cgtggcgacc cggggcacga tccgccccac cctgaccagc
      481 tcctcggtca actgcggcat ggcgctgctc gccttcgacg ccgaggtccc cagtgacgcg
      541 gcgatcaccc gcttctacga caaggtcctg gagcgcttcc cgcacccggc cacccgccgc
      601 cgcgacctga gcggccacga ggtcatccgc gccgccgagg agggcggtga cttcgccgcc
      661 gaacgctggg gcgtcgaccc cgccaacctc acccggatgg agaaggacgg ccgcatcgac
      721 ctggcccgcc acggcggcgc cgcccggctc cgcaaggagc tgccatggct ggtggtgcag
      781 ttcgcgcgga tgcgctttgg caccatcggc cccagcaacc acttcgtcga gctgcagcgc
      841 gtcgaggagg tcttcgacca gcgcaccgcc gaggcgttcg gcgtcaggga aggccagctc
      901 accctgcagt accacggcgg cggtggggtg ctcgccgggc agctcggccg gctgttcggc
      961 cgccgcgaga agatctcccg cccggtcaag gccgagatgg cggtcaccaa gccgctgtac
     1021 cacctcgcct cggcgcggtc gctggccgag ttcaggctgc gccgcgccct ctacttctca
     1081 gacggctgcc cgccggtcga cgccgacggg cccgaaggcg cccggctgct gctcgcccag
     1141 gccgccgcga tgaactacgg cttcgccttc cggctggcca cctacgcgtc cctgcaggag
     1201 ttcgccgcgc aggtcttcgg cgcccgcgac agccacctgg tggtcgactc gccccacaac
     1261 tccatctacg aggaggagct ggacggcgag cgggtcatcc tgcaccgtca caactcctgc
     1321 caggcctacc cggccagccg catggccggc cacccggtgt tcggcaccac cggccaggcg
     1381 atcctgctgc ccggcaccaa caagacctcc tcctacctgt gcgccgccga ggagggcgcc
     1441 ggcgcgagcc tgtggtcggc ctgccacggc gcgggcacga tgatcgacga cttcgtcgcc
     1501 cgcggccgct ccggccccga cccgaagggg cacaccaccc tgcggttcac ctacaagggc
     1561 gacgcgcccg cggtcgtgcc ccacctggac gacaacggcg tcaacgaggc gctcgacatc
     1621 ctgcgggtcc acggcatcgc ccgcccggtc gcgcgcctgc ggccgctcgc ggtgctcaac
     1681 tagggatcca cggagaggca gggagcgcgg agtgcgccgg acggtcaccg acaccaccgc
     1741 ctcgaccggg cgggtcgtcg cccgcaacac cggggcgcag gtggtctgct tcggcgcccg
     1801 ggtgatcgcc gggctgctgg ccgtcgtcct ggtcgcccgg cagggcggcc cgcacgagct
     1861 cggcgtgttc cagttcgggc tgaccctgtc gcagatgttc ggcttcgcca tcgggctcgg
     1921 gctgccgaac ctgctcaccc gcgagatcgc ccgcgacccg gagcacagcc gcgactgggt
     1981 cgggctcggc gtgttcgtgg cgctgtcggc cgggacgatc gtgaccgcgc tgctcaccat
     2041 cgggatgcgg ttcagcggcc ggccgcacga gatggtcgtg tcggtgtccc tggcggcggt
     2101 ggcgctctgc ttcgacaccg ccggacggct cgagttcgcc gccttcgagg gctggcagcg
     2161 gatgtggctg gaggccggcg cgacctgcct gcaggaggcc gccttcatcg ccggcaccat
     2221 cacgatgctg gcgaccgggc acggcgccgc cggcgtgatg gtcgcctacc tgctcagccg
     2281 cgtgctcggc gccttcgccg gctgggtgat cgccagctgg tacctccacg gcccggtcgc
     2341 gccccgcccg ccgcttgcgt tcctgcgtcg cacgctgcgg gtgaccgtcc cgttcgcgct
     2401 cgacgacgcg atgtcgctga cctacatccg catcgacgcg gtgctgctcg ggttcatcaa
     2461 gggcgccgac gcggtcggcc tttaccaggc ggccaccaac ctggtcctgt acctgaacat
     2521 cctcgcccgg atgctcaaca ccgccctcta cccgcggatg agcaaggcct ggcccgaccc
     2581 ggcgcggctg ggccgctggc gggatctgtc gctgcggacc ctcgggctga tctcgctgcc
     2641 ggccatggtc gggtcgttcc tgctcgccca ccagatcttc c
//
LOCUS       DASWDC010000121         5786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1615082, whole genome shotgun sequence.
ACCESSION   DASWDC010000121 DASWDC010000000
VERSION     DASWDC010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615082"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VF995_03765"
     CDS             complement(<1..1049)
                     /locus_tag="VF995_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX9342717.1"
                     /translation="MVTREEVITALGQVMDPEIHKPITELDMVKDVSIDGGVVTVEVL
                     LTIAGCPLRDRITRDVTNAVQPLPGVERVAVGIGTMTDEQRQALVGRLRGGQAQPQKQ
                     ISFWGEASTTRVLAVASGKGGVGKSSVTVNLAAALAAQGHRVAVVDCDIYGFSVPRML
                     GATGRPVGFNGMIMPLEAYGVRVISIGFFIEPGKPVMWRGPMLHRAIQQFLTDVYWGE
                     LDYVLADLPPGTGDVSISLAQMLPGAEMLLVTTPQEAAERVAVLAGKFAQQNQMKIAG
                     VVENMSWFVCPGCEERHLIFGSGGGEELARDLDTKLLGQIPIDLRLREGADAGKPLVV
                     SDPDAPASRELLRIAA"
     gene            1188..2921
                     /locus_tag="VF995_03770"
     CDS             1188..2921
                     /locus_tag="VF995_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9342718.1"
                     /translation="MAEYDAIVAGSGPNGLAAALRLAEAGWTVCVLEASDRIGGGAAT
                     IESTLPGFKHDFGAAFFPMAAISPALGDRDLAPYGLRLLHAPLAAAHPFPGNRAVALG
                     GDVEETAASLDKAHLGDGAAWIDLDRDYGDALEAFLRAQMVRWPLAEALRLAGALGVL
                     PIGGSRGHHGTGSGSFRPRDRAPRTPPVGRATGFARMLLQGAATVAERFESDEARAFL
                     VGPAMHADLEPEMPGSGVYALVLGLLGQRYGMPVAEGGSGAVSAALAARFRELGGTIL
                     TGQRIDRIVVEGGRVLGVEAGDAAFAAGRAVVAALDPRQVIALAGPDVFPVGAVAQIR
                     SFRPGLGTFKVDWALDGPVPWSAEECRRAAVVHVGDTVTAMSRSAWEASHGLLPARPT
                     LILGQQSLADPSRAPAGKHTLWGYAHVPARPSGDAARPRAKGDWERSADPFLDRVEAA
                     IEAHAPGFRELVLARKVWTPADLEAADANLTGGDIAGGSFAVDQQLIFRPGPSWWRWG
                     TPVKGLYLASAAVPPGAGVHGACGDQAALQALADQRRPRLLATGAAAGAALLMARSAR
                     SRSRRRRASKP"
     gene            complement(2946..3791)
                     /locus_tag="VF995_03775"
     CDS             complement(2946..3791)
                     /locus_tag="VF995_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342719.1"
                     /translation="MTELSEVLELLHRGGSGISTLSATMRHWVDMDRQHAAFERAASE
                     SGNQGFSMASMSASRPGPPPHRRQEYQLTFRYRSPDKYRSEQGGNTPYGPPKSLHIRD
                     GTRSWSYFPGQSEAVYQDTSRGEPQLDEFLDPSWLAAAYQLTVTGRTTHEERPAITLT
                     GLPRPIQRAGDHHRLGAEEIRASIDAETGLLLELTSIFEGEPFSIQGLHDLIIGEPLD
                     DDLFQFTPPPGVEVKDVEAQFPMPRRVRWLMPLHWLWLWRMRRKRIAWYGPAREHRKR
                     RWWRS"
     gene            complement(3912..4823)
                     /locus_tag="VF995_03780"
     CDS             complement(3912..4823)
                     /locus_tag="VF995_03780"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX9342720.1"
                     /translation="MSGLDEIVQAVAQVATQVGGSVVGIGRGSGRGSGVVFADGLVVT
                     NAHNIRGDEVTAVFAGGRSAVGQIRGIDVDGDLAVVAVDTEGAAPITWAETPAGVGAA
                     VFALANPGGRQRVTFGLVSAVDRAFRGPRGRRIAGSVEHTAPLARGSSGGPIVNAAGA
                     LLGLNTNRLGDGFYLALPADAELRRRIDALARGESTGPPHLGIGLAPARVARRLRRAV
                     GLPDRDGLLVRVVEEGSPADRAGIQGGDLIVTAGGRPVTEIDDLYGVLDGLAADATLT
                     LGLVRGTEERTVTVSFGAGGAQNVGMA"
     gene            4910..5362
                     /locus_tag="VF995_03785"
     CDS             4910..5362
                     /locus_tag="VF995_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9342721.1"
                     /translation="MPTAETPQRPDAGQPDAGLAEAVALVGDRWTLLIVQALLGGSRR
                     FNDLLADVPGIASNILAQRLKDLERAALVVSQRYSERPPRFAYQLTAVGQELAGALRL
                     LAQWGAQRSGEGDSLRHLECGTPMEARWYCPTCARVVADNEAGDLRFA"
     gene            5397..>5786
                     /locus_tag="VF995_03790"
     CDS             5397..>5786
                     /locus_tag="VF995_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9342722.1"
                     /translation="MKLGFALPHYDFSFPEGRAAGPVAFQRVAGYAERAEALGFAQLW
                     VSDHFWTDLARYGGPSGRQGTLECWTTLAGLAMRTSRVRLGSLVLAAGFRPPSLLAKM
                     VATLDQLAGGRLDVGLGAGWNQDEFVES"
BASE COUNT          747 a         2128 c         2121 g          790 t
ORIGIN      
        1 gcggcgatgc ggagcagctc ccggctggcg ggggcgtcgg ggtcggagac caccagcggc
       61 ttgccggcgt cggcgccctc gcgcagacgc aggtcgatcg ggatctggcc gagcagcttg
      121 gtgtcgaggt cgcgggccag ctcctcgccg ccgccgctgc cgaagatcag gtggcgttcc
      181 tcgcagcccg ggcagacgaa ccagctcatg ttctcgacca cgccggcgat cttcatctgg
      241 ttctgctggg cgaatttgcc ggccaggacc gcgacccgct ccgccgcctc ctgcggggtc
      301 gtgaccagca gcatctcggc gccggggagc atctgcgcca gcgagatcga cacgtcgccg
      361 gtgccgggcg gcaggtcggc gagcacatag tccagctcgc cccagtagac gtcggtgagg
      421 aactgctgga tggcgcggtg cagcatcggc ccgcgccaca tcaccggctt gcccggctca
      481 atgaagaacc cgatcgagat gacccgcacg ccgtaggcct ccagcggcat gatcatcccg
      541 ttgaaaccga ccggccggcc ggtcgcgccg agcatccgcg gcaccgagaa cccgtagatg
      601 tcgcagtcca ccacggcgac gcggtggccc tgcgcggcca gcgccgcggc caggttgacg
      661 gtgaccgacg acttgccgac gccgcccttg ccggaggcga ccgccagcac ccgggtggtg
      721 gacgcctcgc cccagaagct gatctgcttc tgcggctgcg cttggccgcc gcggagccgg
      781 ccgaccagcg cctggcgctg ctcgtcggtc atggtgccga tgccgacggc gacccgctcc
      841 accccgggga ggggctggac cgcgttggtg acgtcgcggg tgatgcggtc gcgcaggggg
      901 cacccggcga tggtcagcag gacctcgacg gtgaccacgc cgccgtcgat cgacacgtct
      961 ttcaccatgt cgagctcggt gatgggcttg tggatctccg ggtccatcac ctgcccgaga
     1021 gcggtgatga cctcctcacg ggtgaccatc gggaagtttc acctcacagg gcgccgcagc
     1081 ggcgccgaac agatcaagac agatcaagaa ggggcgcacc cctacactac ctgcgagtgt
     1141 agcaaccgca cgctccccgc cgagcggtgg gggaggaggc accgaccgtg gcagagtacg
     1201 acgcgatcgt cgctggtagc gggcccaacg ggctggccgc cgcgctgcgg ctggccgagg
     1261 ccggctggac cgtctgcgtg cttgaggcct cagaccggat cggcggcggc gccgccacca
     1321 tcgagtccac cctgcccggc ttcaagcacg acttcggcgc ggcgttcttt cccatggcgg
     1381 cgatctcgcc cgccctgggc gaccgcgacc tcgcccccta cggcctgcgg ctgctgcacg
     1441 cgccgctggc ggcggcgcac ccgttccccg gcaaccgggc ggtcgccctc ggcggcgacg
     1501 tggaggagac cgccgcctcc ctcgacaagg cccacctcgg cgacggcgcc gcctggatcg
     1561 acctcgaccg cgactacggc gacgccctgg aggcgttcct gcgggcccag atggtccgct
     1621 ggccgctggc ggaggccctg cggctggccg gcgccctcgg cgtcctgccc atcggggggt
     1681 cccgcggcca ccatggaaca ggctctgggt ccttccggcc gcgggaccgt gccccccgaa
     1741 ccccccccgt cggccgggcg accggattcg cccgcatgct gctgcagggt gccgccacgg
     1801 tcgcggagcg gttcgagtcg gatgaggccc gcgcgttcct ggtggggccg gcgatgcacg
     1861 ccgacctgga gccggagatg cccgggtccg gcgtgtacgc gctggtcctc gggctgctcg
     1921 ggcagcgcta cggcatgccg gtggccgagg gcggctccgg ggcggtcagc gccgcactcg
     1981 ccgcccggtt ccgggagctg ggcgggacca tcctgaccgg ccagcggatc gaccggatcg
     2041 tggtggaggg cgggcgggtg ctcggggtcg aggcgggcga cgccgcgttc gccgccggcc
     2101 gggcggtggt ggccgcactc gacccgcgcc aggtgatcgc gctggccggg ccggacgtgt
     2161 tcccggtggg cgcggtcgcg cagatccgtt cgttccggcc gggccttggc accttcaagg
     2221 tcgactgggc gctcgacggg ccggtgccct ggtcggccga ggagtgccgc cgcgccgccg
     2281 tggtccacgt cggcgacacc gtcaccgcga tgagccgctc ggcctgggag gccagccacg
     2341 gcctgctgcc ggcccggccg accctgatcc tcggccagca gtcgctcgcc gacccgagcc
     2401 gcgcccccgc cggcaagcac accctgtggg gctacgccca cgtccccgcc cgcccatccg
     2461 gcgacgcggc ccgcccacga gccaagggcg actgggagcg ctccgccgac ccgttcctcg
     2521 accgggtcga ggccgccatc gaggcccacg cccccggctt ccgcgagctg gtcctcgccc
     2581 gcaaggtctg gactccggcc gacctggagg ccgccgacgc caacctgacc ggcggcgaca
     2641 tcgccggcgg ttccttcgcc gtcgaccagc agctcatctt ccgccccggc ccctcctggt
     2701 ggcgctgggg cactcccgtc aagggcctct acctcgccag cgccgccgtc cccccaggcg
     2761 cgggcgtcca cggcgcctgc ggcgaccagg ccgccctcca ggccctcgcc gaccagcgcc
     2821 gcccccgcct gctggcgacc ggcgcggcgg cgggcgccgc gctgctcatg gcaagatccg
     2881 cccgctcgcg ctcccgccga aggcgagctt ccaagccgtg aagggagagg gcgtggcggg
     2941 gcgcgtcagg accgccacca cctgcgcttg cggtgctccc gagcagggcc gtaccaggcg
     3001 atccgcttgc ggcgcatgcg ccacagccac agccagtgca gcggcatcag ccagcggacg
     3061 cgccgaggca tggggaactg ggcctccacg tccttgactt ccacgcctgg cggcggggtg
     3121 aactggaaca ggtcgtcatc gagcggctca ccgatgatca ggtcgtgcag gccctggatg
     3181 gagaacggct cgccttcgaa gatgctggtc agctccaaca gcagaccggt ctccgcgtcg
     3241 atgctggcgc ggatctcctc tgcgccgagc cggtggtggt caccggcgcg ctggatcggc
     3301 cgtgggaggc ccgtgagggt gatcgccggg cgttcttcgt gggtcgttcg accagtgacg
     3361 gtgagctggt aggcggcggc caaccaggac gggtcgagga actcgtcgag ctgcggttcc
     3421 ccccttgagg tgtcctggta caccgcctcg ctctgccccg ggaagtagga ccagcttcgc
     3481 gtcccgtcgc ggatgtgaag cgacttcggg gggccgtatg gcgtgttgcc cccctgttcg
     3541 ctgcggtact tgtcgggaga ccggtagcgg aaggtgagct ggtactcctg gcgcctgtgc
     3601 gggggagggc cgggacggga ggcgctcatc gatgccatgc tgaacccctg gttgcccgac
     3661 tcggaggccg ctcgctcgaa ggcggcgtgc tggcgatcca tgtcgaccca gtggcgcatc
     3721 gtggccgaga gcgtgctgat cccgctcccg ccccggtgca gcagctccag gacctcgctc
     3781 agctcagtca tggcgaaaca gtaggcggtg acgggcgcgc cgtacaggac ttttcacccc
     3841 agggaggtcc cgtgcagcgg cccgccaccg cctacccgct cagcgggttc gcgcaggctc
     3901 aggccattgc cctaggccat gccgacgttc tgggcgccgc cggcgccgaa gctgaccgtg
     3961 acggtgcgct cctcggtgcc tcggaccagg ccgagggtga gggtggcgtc ggcggcgagg
     4021 ccgtcgagca ccccgtagag gtcgtcgatc tcggtgaccg ggcggccgcc ggcggtgacg
     4081 atcaggtcgc cgccctggat gccggcgcgg tcggcggggc tgccctcctc gaccacgcgg
     4141 accagcaggc cgtcgcggtc ggggaggccg acggcgcggc gcaggcgcct ggcgacccgg
     4201 gcgggcgcca ggccgatgcc gaggtgcggc gggccggtgg actcgccacg ggcgagcgcg
     4261 tcgatccggc ggcgcagctc cgcgtcggcg ggaagcgcca ggtagaagcc gtccccgagc
     4321 cggttggtgt tgaggccgag cagcgcgccg gcagcgttca cgatcggccc gccggaggag
     4381 ccgcgggcga gcggggcggt gtgctcgacg ctgccggcga tgcggcgccc gcgcgggccc
     4441 cggaacgccc ggtcgaccgc cgagaccagc ccgaaggtga cccgctggcg gccaccagga
     4501 ttggccaggg cgaacaccgc cgcgccgacc ccggcgggcg tctcggccca ggtgatcggg
     4561 gccgcaccct cggtgtcgac cgcgaccacc gcgaggtcgc cgtcgacgtc gatcccgcgg
     4621 atctgcccga cggcgctgcg gccgcccgcg aagacggcgg tgacctcgtc cccgcggatg
     4681 ttgtgggcgt tggtcactac cagcccgtcg gcgaagacca cccccgaccc ccggccggac
     4741 cctcggccga tgccgaccac cgacccgccg acctgggtcg cgacctgggc gaccgcctgc
     4801 acgatctcgt cgagtccact catcctgcgc ctcccgagag gggtaactag cagattgcaa
     4861 gttactactc tctcgtcgag catggcaaga attccgtacg atatgcggta tgccaacagc
     4921 ggaaacaccc cagcgccccg acgccgggca gcccgacgcc gggctcgccg aagccgtcgc
     4981 cctggtcggc gaccgctgga cgctgctgat cgtgcaggcc ctgctcggcg ggtcgcggcg
     5041 cttcaacgac ctgctcgcgg acgtgcccgg gatcgcgtcg aacatccttg cccagcgcct
     5101 caaggacctg gagcgcgccg cgctggtggt cagccagcgc tactccgagc ggccgccccg
     5161 cttcgcctac cagctcaccg ccgtcggcca ggagctggcg ggcgccctgc ggctgctcgc
     5221 ccagtggggc gcccagcgct ccggcgaggg cgacagcctg cgccacctgg agtgcggcac
     5281 cccgatggag gcccgctggt actgcccgac ctgcgctcgc gtggtcgccg acaatgaggc
     5341 gggagacctg cgcttcgcgt agtcgagggg gaatggcgaa ccgttcagga ggcgtgatga
     5401 agctcgggtt tgcgctgccg cactacgact tctcgttccc tgaagggcgt gccgccgggc
     5461 cggtcgcctt ccagcgggtg gccggctacg cggagcgggc cgaggcgctc gggttcgcgc
     5521 agctgtgggt ctccgaccac ttctggacgg atctggcgcg ctacggcggg ccgtccggcc
     5581 gccagggcac cctggagtgc tggaccacgc tcgcggggct ggcgatgcgg accagcaggg
     5641 tgcgcctggg gagcctggtg ctggccgccg ggttccggcc gccgagcctg ctcgccaaga
     5701 tggtcgccac cctcgaccag cttgcgggcg gccggctcga cgtcggcctc ggcgccggct
     5761 ggaaccagga cgagttcgtc gagagc
//
LOCUS       DASWDC010000122         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_98820, whole genome shotgun sequence.
ACCESSION   DASWDC010000122 DASWDC010000000
VERSION     DASWDC010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98820"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VF995_03795"
     CDS             <1..811
                     /locus_tag="VF995_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342723.1"
                     /translation="SWRHHPALALPDAADSTLSGLLAATERRDDPAASDQILAALAQL
                     ARGNPDDATHDPLAARTLLQTLPPGAKALARRLRWLGDPQERAAAVTATLYEQIRTYP
                     IQRRPARIAANLLADTHQRLLRRAGSSSSSSSSSAPHRRQVQVGGGTVAVPVVSLDEL
                     AAHGDAMLPLPAPSQPCPAEELLALLAWAVRGGWLTPAQAALIGQSRIADIPAEELGA
                     LAGLGAHSLRRRRQRAEQALRHAATASALSPEDLARLAPQLACRVPATICA"
     gene            complement(957..1892)
                     /gene="mdh"
                     /locus_tag="VF995_03800"
     CDS             complement(957..1892)
                     /gene="mdh"
                     /locus_tag="VF995_03800"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX9342724.1"
                     /translation="MKVTVVGAGKYGSTTAQRIAEKDLCDEVVMTDIVEGLPQGLALD
                     MTQARSIEHFETRIVGTNGYEETAGSDVCVITAGLPRKPGMSRMDLLETNARIVGEVT
                     RKLVERSPDTILIVVSNPLDEMTALAAQVSGLPHQRVMGQAGMLDTARLKHFVAEELG
                     TSPSGVEAITLGSHGDTMVPVPSQVTVDGKPLTELADPATVERLVQRTRDGGAEVVAL
                     LKSGSAYYAPSSAATAMVEAVLTDSKALMPVCAWVNGEYGIDGVYLGVPARLGRAGVE
                     EIVELPLTDAEVANLRDAAEAVRSKQADVAKLVTG"
     gene            complement(1889..>2154)
                     /locus_tag="VF995_03805"
     CDS             complement(1889..>2154)
                     /locus_tag="VF995_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX9342725.1"
                     /translation="FEATHGSAPKYKGQNKVNPMAMMLSGMLMLRHLGESAAADRLER
                     ALAAVIAEGRDVTYDMKPRRDDPSAVGTSQVADAVIARMEEGV"
BASE COUNT          278 a          839 c          750 g          287 t
ORIGIN      
        1 cagctggcgc caccaccccg ccctggccct cccggacgcc gcggacagca ccctcagcgg
       61 cctgctcgcg gccaccgaac gccgcgacga ccccgccgcc agcgaccaga tcctcgccgc
      121 cctcgcccag ctcgcccgcg gcaaccccga cgacgccacc catgacccgc tggccgcccg
      181 cacgctgctg cagacgctgc cgcccggcgc caaagccctc gcccgccgcc tccgctggct
      241 cggcgacccc caggaacgcg ccgccgccgt caccgccacc ctctacgagc agatccgcac
      301 ctaccccatc cagcgccgcc ccgcccgcat cgccgccaac ctgctcgccg acacccacca
      361 gcggctgctc cgccgcgccg gctccagctc cagctccagc tccagcagcg ccccgcaccg
      421 ccgccaggtc caggtaggcg gcggcaccgt ggccgtgccg gtggtgtcgc tggacgagct
      481 ggccgcccac ggcgacgcca tgctgcccct accagccccc agccagccct gcccggccga
      541 ggagctgctg gcgctcctgg cctgggcggt ccgcggcggc tggctcaccc ccgcccaggc
      601 cgccctgatc ggccagagcc gcatcgccga catccccgcc gaggaactgg gcgccctggc
      661 cgggctcggc gcccacagcc tgcggcgccg ccgccagcgc gccgagcagg ccctcaggca
      721 cgcggcgacc gcgtccgccc tctccccgga ggaccttgcg cggctggcgc cgcaactggc
      781 gtgtcgcgtc ccggccacga tctgcgcata gccaaagtag acgcccacca ccacaagcaa
      841 cgcagcgatc agcatccacc cacagcaggg tgggcgtcgg ggccaggccg gcagcggcgg
      901 accggaaacg tgcccggcct ggccccctcc aattcccttc gagcggtgcg gcgctgtcag
      961 ccggtgacga gcttggcgac gtcggcctgc ttggagcgga ccgcctcggc ggcgtcgcgg
     1021 aggttggcca cctcggcgtc ggtgagcggc agctcgacga tctcctcgac gccggcacgg
     1081 ccgaggcggg cggggacgcc gaggtagacg ccgtcgatgc cgtactcgcc gttgacccag
     1141 gcgcagaccg gcatgagcgc cttggagtcg gtgaggacgg cctcgaccat ggcggtggcg
     1201 gcgctggagg gggcgtagta cgcggaaccc gacttgagca gggcgaccac ctcggcgccg
     1261 ccgtcgcggg tgcgctgcac cagccgctcg acggtggcgg ggtcggccag ctcggtgagc
     1321 ggcttgccgt cgacggtgac ctggctcggg accgggacca tggtgtcgcc gtgggagccg
     1381 agggtgatcg cctcgacgcc gctcgggctg gtgcccagct cctcggcgac gaagtgcttg
     1441 aggcgggcgg tgtcgagcat gccggcctgg cccatgaccc gctggtgcgg gaggccggag
     1501 acctgcgcgg ccagcgcggt catctcgtcg agcgggttgg agaccacgat caggatcgtg
     1561 tcgggtgagc gctcgaccag cttgcgggtg acctccccga cgatcctggc gttggtctcc
     1621 agcaggtcca tgcggctcat gccgggcttg cggggcaggc cggcggtgat cacgcagacg
     1681 tcggagccgg cggtctcctc gtagccgttg gtgccgacga tccgggtctc gaagtgctcg
     1741 atcgagcggg cctgcgtcat gtcgagggcg aggccctggg gcagcccctc gacgatgtcg
     1801 gtcatcacga cctcgtcgca gaggtccttc tcggcgatcc gctgcgcggt ggtggagccg
     1861 tacttgccgg ccccgacgac ggtgaccttc ataccccttc ctccatcctg gcgatgaccg
     1921 cgtccgcgac ctggctggtg ccgacggcgc tggggtcgtc gcggcgcggc ttcatgtcgt
     1981 aggtgacgtc cctgccctcg gcgatcacgg ccgccagggc gcgctccagc cggtcggcgg
     2041 cggcgctctc gccgaggtgg cgcagcatca gcatgccgga gagcatcatc gccatcgggt
     2101 tgaccttgtt ctgccccttg tacttggggg cggaaccatg ggtggcctcg aaca
//
LOCUS       DASWDC010000123         4036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_280134, whole genome shotgun sequence.
ACCESSION   DASWDC010000123 DASWDC010000000
VERSION     DASWDC010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280134"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VF995_03810"
     CDS             <1..501
                     /locus_tag="VF995_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9342726.1"
                     /translation="DGRMTVRRRRFAPDDLDGMFLCVAASPDPVERAAIHREARARQV
                     LVNVMDDPAHCDFAAPAVVRRGDLAIAVSTGGRSPALASRLREQLEGRYGPEWAALLD
                     LLAEVRAQTIAALPDVGERIRRWRGALRLDELEALLRDGRQEEARRRLLDRLTARDAD
                     GEGGGA"
     gene            494..1255
                     /gene="cobA"
                     /locus_tag="VF995_03815"
     CDS             494..1255
                     /gene="cobA"
                     /locus_tag="VF995_03815"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX9342727.1"
                     /translation="MPEARAGIVYLVGAGPGDPGLLTLRGAALLRGADVVVHDRLAPP
                     ELLGLAPEGAEVVDVGKRPGDASRSQEAVNKLLVERALQGKLVVRLKGGDPFVFGRGG
                     EEALACAEAGVPFEVVPGVSAAIAAPAYAGVPVTQRAMAASFAVVTASRAGGINPDLK
                     RVATAVDTLVLLMAAGRLREVCEALIAAGRPGDDPALVVQWAATPRQRSVSGSLAALP
                     DLAADEGLGAPATLVVGAVASLAEQLRWFGNSPQI"
     gene            1528..2379
                     /locus_tag="VF995_03820"
     CDS             1528..2379
                     /locus_tag="VF995_03820"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX9342728.1"
                     /translation="MAVVATEWSPAVALPRLRPFEIEAAAERLESCSAQEVLAWAVAT
                     FGDHFAIVTSFQREGMAILDMALRLDRSVRVLTIDTGRLPAETYEMIDTVRARYGVEV
                     EVIAPDTASLEAMQGLHGPNLFRRDRSLRRLCCHIRKVEPLERALEGLGAWATGLRRD
                     GGLARAGIAKVELDEAHGGIVKLNPLADWTRERVVAYTTSRNLPEHPLYAEGYTSIGC
                     APCTRPTAAGESERAGRWWWEADELRECGLHHRSASQRLDLALAEVHTRAAAGTATAT
                     ATTRRTA"
     gene            2376..3947
                     /locus_tag="VF995_03825"
     CDS             2376..3947
                     /locus_tag="VF995_03825"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX9342729.1"
                     /translation="MTNQVEQVKAALDPIDPEVEQRIRRYARDGYQSIDPEDLKVRFR
                     WWGLYTQRPEAAGYFMMRIRVPGGALTAEQLDTIGWITRQRTRNLADVTDRQNIQLHW
                     VVIQDVPKIWDTLATVGLATNQTCGDTVRNILGCPVAGADANEVFDATPDLRAAEARL
                     ARTQEFSNLPRKYKLSITGCADHCALHEINDGGLVGVIHPDGREGYDVYVGGGLSSSP
                     RFGERLGVFVPRAEAPPFLVALTSLFRDHGGRDKRTRARLKFLVAAWGVEKLREVLED
                     DYLGHPLEDGPQASPSASVHRDHVGVHRQKDGRFYVGVAPLVGRVSGDQLIEVARIAR
                     ELGSGQVRLTTQQKLVVLGVPGDSVDEAVSRLKAIGLPADPSPFHRGAIACTGIQFCK
                     LALVETKQRAMDLVQALEARYPDFDGKVRLNVNGCPNSCARYQIADLGLAGGESAGDG
                     NYQLHLGGDLGEFKALGQRVRERVLADHSEASLTSILDAYLDGRHDGESLQAWLRRQA
                     PETLAAISKPALAGS"
BASE COUNT          545 a         1509 c         1446 g          536 t
ORIGIN      
        1 gacggccgca tgaccgtgcg gcggcggcgg ttcgccccgg acgacctcga cgggatgttc
       61 ctgtgcgtgg ctgcctcacc cgacccggtg gagcgggcag cgatccatcg ggaggcccgg
      121 gcgcggcagg tgctcgtcaa cgtgatggac gacccggcgc actgcgactt cgccgcgccg
      181 gcggtggtac ggcgcggcga cctggcgatc gcggtctcga ccggggggcg ctccccggcg
      241 ctggcgagcc gcctgcggga gcagctggag ggacgctacg ggcccgagtg ggcggcgctg
      301 ctcgacctgc tcgccgaggt gagagcccag acgatcgccg cgttgccgga cgtcggcgag
      361 cggatccgcc gctggcgtgg ggcgctgcgc ctcgacgagc tggaggcgct gctccgcgac
      421 ggccgccagg aggaggcgcg gcgccgcctg ctcgaccgcc tcaccgccag ggatgccgat
      481 ggggagggcg gcggtgcctg aggccagggc cgggatcgtg tacctggtcg gcgctggacc
      541 tggggatccg gggctgctga cgctgcgcgg cgccgcgctg ctgcgaggtg cggacgtggt
      601 ggtccacgac cggctcgcac cgcccgagct gctcggcctc gccccggagg gtgcggaggt
      661 cgtcgacgtg ggcaagcggc ccggggacgc cagccgcagc caggaggccg tcaacaagct
      721 gctggtggag cgggctctgc agggcaagct ggtggtgcgg ctcaagggcg gcgacccctt
      781 cgtcttcggg cgcggcggcg aggaggcgct ggcctgcgcc gaggccggcg tccccttcga
      841 ggtggtcccg ggggtgagcg ccgcgatcgc ggcgcccgcc tacgccgggg tgccggtcac
      901 ccagcgggcg atggcggcga gcttcgcggt ggtgaccgcg agccgggccg gtgggatcaa
      961 cccggacctc aagcgcgtcg ccaccgccgt ggacacgctg gtgctgctga tggccgccgg
     1021 gcggctgcgg gaggtctgcg aggcgctgat cgcggccgga cggccgggtg acgacccagc
     1081 gctggtggtg cagtgggccg cgaccccgcg ccagcgctcc gtgtcgggat cccttgccgc
     1141 gctgcccgac ctcgccgccg acgagggcct cggagcgccc gccacgctgg tggtcggggc
     1201 ggtcgccagc ctcgccgagc agctccgctg gttcggcaat tccccacaga tttagtcgag
     1261 aaatggggcg gtcaggaccc agccagcatt tcttgacagc ttgcccctct ctccggcacg
     1321 gtttatgcgc gatgactgtc ctcctgctga cacgcaaccg cgccttggac cacgggcacg
     1381 tcgtttgctg cgcctgtcgg caggcccggc ggtcctgccg cgcaacgtag cgcagcacct
     1441 cgctccctcg accgccgggc ctgaccggtc caccgcattc gcttgcgttt cgctaccgct
     1501 acccgcgtgc cgagaggagc ccccactgtg gccgtcgtcg ccactgaatg gtcacccgcc
     1561 gtcgccctgc cccgcctccg cccgttcgag atcgaagccg ccgccgagcg gctcgagtcg
     1621 tgttcggcgc aggaggtgct tgcctgggcg gtcgccacct tcggggacca tttcgcgatc
     1681 gtgacgtcgt ttcagcgcga gggcatggcg atcctcgaca tggcgttgcg gctcgaccgg
     1741 tcggttcggg tgctgaccat cgacaccggg cgcctgcccg ccgagaccta cgagatgatc
     1801 gacacggtcc gcgcccgcta cggcgtcgag gtcgaggtga tcgcccctga caccgccagc
     1861 ctggaggcga tgcagggtct ccacggcccc aacctgttcc gccgcgaccg ctcgctgcgg
     1921 cggctctgct gccacatccg caaggtcgaa ccgctggagc gcgccctgga gggcctgggc
     1981 gcctgggcga ccgggctgcg ccgcgacggc ggcctcgccc gcgccggcat cgccaaggtc
     2041 gaactggacg aggcccacgg cgggatcgtg aagctgaacc cgctggccga ctggacccgc
     2101 gagcgggtcg tggcctacac cacttccagg aacctgccgg agcaccccct ctacgccgag
     2161 ggctacacca gcatcggctg cgccccctgc acccgcccga ccgctgccgg cgagtccgaa
     2221 cgcgccggcc gctggtggtg ggaggccgac gagctgcgcg aatgcgggct gcaccaccgc
     2281 agcgcctcgc agcgcctcga cctggccctc gctgaggtgc acacccgggc cgccgccggc
     2341 accgccaccg ccaccgcgac caccaggaga accgcatgac caaccaggtg gagcaggtca
     2401 aagccgcgct cgacccgatc gaccccgagg tcgaacagcg catccgccgc tacgcccgcg
     2461 acggctacca gagcatcgac cccgaggacc tcaaggtccg cttccgctgg tgggggctgt
     2521 atacgcagcg tcccgaggcg gcgggctact tcatgatgcg catccgcgtc cccggcggcg
     2581 cgctcaccgc tgagcaactg gacaccatcg gctggatcac caggcagcgc acccgcaacc
     2641 tcgccgacgt caccgaccgg cagaacatcc agcttcactg ggtggtgatc caggacgtcc
     2701 ccaagatctg ggacaccctc gccaccgtcg gtctggccac caaccagacc tgtggcgaca
     2761 ccgtccgcaa catcctcggc tgtcccgtcg ccggggccga cgcgaacgag gtcttcgacg
     2821 ccaccccgga cctgcgcgcg gcggaggcgc ggctggcccg cacccaggag ttctccaacc
     2881 tgccgcgcaa gtacaagctg tcgatcaccg gctgcgccga ccactgcgcc ctccacgaga
     2941 tcaacgacgg cggcctggtc ggggtcatcc atcccgacgg gcgcgagggc tacgacgtct
     3001 acgtcggcgg cgggctgtcc agctcgcccc ggttcggcga gcggctcggc gtgttcgtcc
     3061 cccgcgccga ggcgcccccg ttcctggtcg cgctcacctc gctgttccgc gaccacggcg
     3121 gccgcgacaa gcgcacccgc gcgcggctga agttcctggt ggccgcctgg ggtgtcgaga
     3181 agctccgcga ggtgctggag gacgactacc tcggacatcc gctggaggat ggcccgcagg
     3241 cgtccccgtc ggcgagcgtg caccgcgacc acgtcggcgt ccaccgccag aaggacggcc
     3301 gcttctacgt cggcgtcgcg ccgctggtcg ggcgggtctc cggcgaccag ctgatcgagg
     3361 tggcccgcat cgcccgcgag ctcggcagcg gccaggtgcg gctcaccacc cagcagaagc
     3421 tcgtcgtgct cggcgtcccg ggtgacagcg tcgacgaggc ggtgtcgcgg ctcaaggcga
     3481 tcggcctgcc ggccgacccg agcccgttcc accggggcgc gatcgcctgc accggcatcc
     3541 agttctgcaa gctcgccctg gtcgagacca agcagcgggc catggacctg gtccaggcgc
     3601 tggaggcccg ctacccggac ttcgacggca aggtccgcct caacgtcaac ggctgcccca
     3661 actcctgcgc ccgctaccag atcgccgacc tcggcctggc cggcggcgag agcgccgggg
     3721 acggcaacta ccagctgcac ctcggcggcg acctcggcga gttcaaagcg cttggccagc
     3781 gggtccgcga gcgggtcctc gccgaccaca gcgaggcctc cctgaccagc atcctcgacg
     3841 cctacctgga cgggcgccac gacggcgagt cgctgcaggc gtggctgcgg cgccaggccc
     3901 ccgagacgct ggcggccatc agcaagccgg cgctggccgg cagctgagcc ttcacaccag
     3961 ccgcagccaa tcccgatccg ggagagggcc gggcgccccc cgcgggaccg tgtggctagc
     4021 ggcgtgcgcc tgggct
//
LOCUS       DASWDC010000124        19174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_934328, whole genome shotgun sequence.
ACCESSION   DASWDC010000124 DASWDC010000000
VERSION     DASWDC010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19174
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934328"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VF995_03830"
     CDS             complement(<1..599)
                     /locus_tag="VF995_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007734646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342730.1"
                     /translation="MQLSEVGALLDPTPLPIETGYERFPNGVLHVAARTDLHGCSGEM
                     FEWWFRFRPDTQQYIWWHPVDHQFSDWTETREGTHIGSIHVVVEEFTGLPAEKLSIQF
                     REPSEFFDAGAYAAARASGAVSGAVLGRVGLSHTPDRDPSGAIIGGRLLHIVRDTPWG
                     AALRSHFYLGQDLPAQGLSPAEITAAFSDEFGQALLQHA"
     gene            690..1523
                     /locus_tag="VF995_03835"
     CDS             690..1523
                     /locus_tag="VF995_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX9342731.1"
                     /translation="MSVVITVAPTGPIATKADNPSLPTTPEEIAKAAGDAYSLGASVA
                     HVHLRDEQERPTADLRIGRRVMDMIADRCPILVQLSTGVGLSVPFEEREQLVELRPRM
                     ATLNPCSMSFGEGEFRNPPTGVRRLATRMRELGVKPELEIYDTGHLDACLRLRDEGLL
                     DEPLQFSIVLGVRGGMAATPENLLTMVRRLPEGAIWQVIAVGRASLPLTAVGLAIGGN
                     ARAGMEDTIHLAKGKLTDGNDPLVQRAVGLAHDLGMEVAGTDGTEKLLQLPRPIEPAT
                     S"
     gene            complement(1527..3620)
                     /locus_tag="VF995_03840"
     CDS             complement(1527..3620)
                     /locus_tag="VF995_03840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9342732.1"
                     /translation="MTTTIDKPTAWGSAAPASGSRRTGLLRRLVRGRESDQPWVRPAL
                     LALLAATALLYLWSLGASGYANTFYAAAVQAGSKSWKAFFFGSSDSSNFITVDKTPAS
                     LWVMDISARIFGVNSWSLLVPQALEGVATVGILYAAVRRWFSAPAALLAGAVMALTPV
                     AVLMFRFNNPDALLTLLLVAGAYAMVRAVENGRTSWLVLAASCVGFGYLTKMLQALLV
                     VPAFALVYLVAGPTPLRRRIGQLLMAGVAMVVSAGWWVAIVAMIPAADRPYIGGSQHN
                     SILELTFGYNGFGRLTGNETGSVGGGGAGGGGRWGTTGLFRLFSEMGGEVTWLLPAAL
                     ILLGAGLWLTRRTPRADRTRAALLLWGGWLVVTGLTFSYAAGIIHPYYTVALAPAIGA
                     VVGIGAAFFWRYRSHFVARAVLAASLAATGWWSFTLLERVSGWLPWLAPVVLLGSLAV
                     GALLLAMPSLRFTRRAALAVAAAGLIVALAAPAAYSMSTAAQPHSGAIPSVGPASAGR
                     LGFGGGRPGGGGNFGTNRQFGNGQGQAPGGFPFGGTNGNGQGNANGQGTTGGPGNFAN
                     PGGQGGRGGIGSLLNGSTPSSQLVALLKQGASGYRWTAAAVGSNSAAGVQLAAGEPIM
                     AIGGFNGTDPTPTLAQFKQYVAQGKIHYFIGGSGLGSGQGGSSTSSQITSWVQQTFKS
                     TTVGGTTVYDLTTTA"
     gene            complement(3617..5005)
                     /locus_tag="VF995_03845"
     CDS             complement(3617..5005)
                     /locus_tag="VF995_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HEX9342733.1"
                     /translation="MTRTTEHTVTGEGEGKADRRSQMAAPLAAPLAAGAAVSQGASAA
                     PSAAPAVEVVIPVYNEERDLERSVTRLHTYLAESFPFSFRITIADNASSDQTWPIARV
                     LSASLPGVRAVHLDEKGRGRALRAVWGASDATVLAYMDVDLSTDLSALLPLVAPLLSG
                     HSDVAIGTRLNASSRVVRGPKREIISRCYNLLLRTTLRARFSDAQCGFKAIRADRARE
                     LLPLVEDTAWFFDTELLVLAERAGLRIYEVPVDWVDDPDSRVDIVATALADLRGVRRL
                     AWGLASGKIPVRTLRQTATAHGPEGAPDPLPGVPSGLARQLLRFAVVGVASTLAYLVL
                     FVLLHDWMGAQVANALALLLTAVGNTAVNRRVTFAVRGVARAGRHHLQGLAVFALGLV
                     LTSSTLAILHAVTRTPARMLELGVLVAANLAATLLRFVLFRSWVFPTTPTTTAPPPLT
                     PPPTPSMQRIHP"
     gene            complement(5115..5654)
                     /locus_tag="VF995_03850"
     CDS             complement(5115..5654)
                     /locus_tag="VF995_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9342734.1"
                     /translation="MQIRRVVTGHTAEGKAVVVSDGPAPRSHDFVHIPMMATTMLWAT
                     EPGEPISRDGADPTAAVRSQVPEPGGTCVVIVTFPPDSVYADPGFDPVAADAEGRLAT
                     PGIADRFEPDNPGMHWTPTVDYQIVLDGELWLELDDGALTHLRQGDVVIQNGTRHGWR
                     NHTDRPATLAVFHVGAERV"
     gene            complement(5676..6650)
                     /locus_tag="VF995_03855"
     CDS             complement(5676..6650)
                     /locus_tag="VF995_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9342735.1"
                     /translation="MELGCLFPPTMETPAHIERAEALGYRRALVYDSPAFLADPWVTL
                     ALAAARTSRIGLGVGVITPRLRHVVASAGALATLSTLAPGRIEVGVGAGFTSQLMLGK
                     PPARWAEVEAYTIALRALLRGEEVEWDGSVIGLRYGTRAGVRLPADLPIRIAAHGPRG
                     YAAAERVGDGIITNLSHRGSNPLPPDMGRVQVLYYGTVLEEGEDPGSERVLHAAGPYA
                     AFQLHIGDHGIVAEAPESAAFHQAVARLDPRRRHLEVHRGHLIEVTDLERPLVTGTLI
                     AGTTGTGTPGEVRRRLDEIAASGVEGVLYGPMGPDIPRELAAFAAVWD"
     gene            6824..7639
                     /locus_tag="VF995_03860"
     CDS             6824..7639
                     /locus_tag="VF995_03860"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007989199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9342736.1"
                     /translation="MVVEPATGLVFVELSHVWGAHTPAFPGYKDIQVHRLSTHAQYGV
                     LTQRIVTVMHHGTHVNAPLHLVAGGESVGDLPLDRFFGNGVVLPVPKGEWELIEPDDL
                     EAAQAASGEQVQPGDIVLLNTGWHHGWSDSMEYFGHSPGLSEAAAQWLADHQVKLAGI
                     DTATVDHPMATSLARNYRGVGPIVRELPRRWRARTGHEPQEDFPAWMPAHRLLLGAGI
                     PTIENVGGDLDSVTGMRCAFHAIPWYWPEGDACVIRLVAILDPTGEYRLEKGS"
     gene            7644..8474
                     /locus_tag="VF995_03865"
     CDS             7644..8474
                     /locus_tag="VF995_03865"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003916848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9342737.1"
                     /translation="MAGTPSPSTSTDGLRFYSLSHPLGHNMPQWPSPMATPLVTSRLY
                     YHAMHGFCTYEYDGIMHRGTHMDVPVHVTANTPYLLDFPLWRFFGTGVAISVPKGKWG
                     VVTPDDLERAEPKIQPGDIVMINTGFHHYFGDNDRYYAYSGGLYKEAAQWLVDKQVKL
                     VGIDVQALDHALGTRMVEHGPGPTHPHLMEEYRAETGRDVMMDFPDWEPAHKTMLLAG
                     IPGVENVGGDLDAVTGKRCTFMVFPWRWANGDGCSVRVVAVIDPEQKFRIPTGTPVGA
                     "
     gene            8476..9300
                     /locus_tag="VF995_03870"
     CDS             8476..9300
                     /locus_tag="VF995_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007989193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9342738.1"
                     /translation="MVADDALHNPLSLFDVSGKVTVITGASGALGHAVAVALAALGSK
                     LLLVSGSEQALEAVAAEARETAAARNSGGEVQTLVRRPETLEDAEVIISTAVAAFGQV
                     DSMLVASGLNQPGPIEEQPYEDWQAVMDANVRGPWLLAKVFGPHVFARGGKGKLVLIS
                     SVRGRHGHPVGYSAYCTSKGATDALTRTLAVEWATRGINVNAIAPAVFRSTLTDWIFA
                     DTKLGEEARARNYSRIPMKRLGEPQDFIGIALYLLSDASDWVTGQIIYVDGGYTAA"
     gene            9297..10235
                     /locus_tag="VF995_03875"
     CDS             9297..10235
                     /locus_tag="VF995_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9342739.1"
                     /translation="MTGEPRVSIVGAGLMGAGIAQVFVAAGLEVSIYDPAPSALASAP
                     DRVRAGLELLDPERDSGEALRRLALNTDLAALVEGADLVIEAGPERVEVKRAIFADLD
                     RLAPASAVLTSNTSAIPIKEIARDCEQPERVIGTHFWNPPALVPLVEVVQSEASEPTL
                     VEWTIRLLGQVGMKPVHVRSDTPGFVGNRMQHALKREAIALVAEGVCDAETVDVVARF
                     GFGARLGILGPLEQSDLVGLGLLLDIHKTLMPALNRTAVPHPYLVEKVRRGETGAAVG
                     HGFREWTPEEAAARQAEVTRELVAAARRRREAEAQQ"
     gene            10297..11094
                     /locus_tag="VF995_03880"
     CDS             10297..11094
                     /locus_tag="VF995_03880"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9342740.1"
                     /translation="MTRLAGKVALVTGAGGGIGRAAAVRFAEEGARVGITDQDAANLE
                     ETAAAVKAEFAKVAGTGDSGAGDGVVQVVGDIVDPATIDQLATQTVERFGQIDVLFNN
                     VGILILKSLADTTPEDFDRLMHVNVLSHLLGIQRVAPEMRRAGGGSIINVASVGGLVA
                     LPNVSAYSASKSAVIGLTRSAAVEFGPEIRVNAVCPGGVDTPMAQRHLASFEDKQAAM
                     KLLTGRQIIKRYAEPREIADVVVFLASDEASFVTGTAIAADAGHTTW"
     gene            11135..11722
                     /locus_tag="VF995_03885"
     CDS             11135..11722
                     /locus_tag="VF995_03885"
                     /inference="COORDINATES: protein motif:HMM:NF036707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342741.1"
                     /translation="MVTAPEWGDRIEVLSPTRVRVAALDRLGGITPAMIDWWFGRMDE
                     ETYFRFHPVDHQKFAWVRGKQPGSYIGATHLTYHCYGGLGPVLRSHITFIPPRELLDE
                     GLFEAHGVGAAVCAVVHLLDEHDRPEQEEAGRFMHVAVERGYGTELRSCWWLTVGPDS
                     DVELLTTGRLRHVHEEFAYLTGFLPGLYKEEVGQR"
     gene            11719..12522
                     /locus_tag="VF995_03890"
     CDS             11719..12522
                     /locus_tag="VF995_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX9342742.1"
                     /translation="MTLRFAANLSILWTDRPFAERFERAAAAGFDTVELWWPGLEAAE
                     ELPELTARNRLGLALLNFDGGDMAAGERGLANDPERHEQLRANIPAALRIAEACGCTR
                     LNLLVGQQHDRATLEQQHGWAAGEVAWAADQAAPRGCEVLIEAINSFENGPYLLTTTA
                     AAADFAQRVGRPNVRLQYDAYHMQRMEGNLTATIDTYWPLVSHIQIADAPGRNEPGTG
                     EINYRYVLDHIERKGYDGFVGLEYRPSTGNADDSFGWLDTLGRPRGSRT"
     gene            12519..13463
                     /locus_tag="VF995_03895"
     CDS             12519..13463
                     /locus_tag="VF995_03895"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9342743.1"
                     /translation="MSSNSSGTVGFIGLGVMGRPMAENLLRAGVDLVVWNRSQEPQEA
                     LVAQGATGVVTIQDVGKRADVIITMLADDLAVRDVILHRLVLLPAARPGSLIIDMSTV
                     SPAISREIAERAAGKGIHTLDAPVSGGDVGAREGTLSIMVGGEAADLERARPLLEILG
                     GSIVHVGPAGAGQVVKACNQLLVAITIAGVSEALVLGSKLGVAPGAILEVLSRGLAGN
                     RVMELRRDNFLEHRFTPGFKVDLHHKDLDIALASGGQANVPLALTAAAQQLLRQLRAA
                     GRGGEDHSAILAAVEAQAAHRIGTPTATGATEEQGESP"
     gene            13460..14350
                     /locus_tag="VF995_03900"
     CDS             13460..14350
                     /locus_tag="VF995_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9342744.1"
                     /translation="MTIPTGSAFINALVSGLLLGGLFAVTALGLSLVFGVMRLINLVH
                     GELIVFGAYLALELTRHSHVTPLLTIVVVAPVLFLVAAPVQRLLLEPLMAKGPEPALL
                     TTFGLSVIAQNLFVLVWSGDVQSLPARYATGAVKVLGLRIADMYLISFAVALVLCGLL
                     HLMLQRTRLGLRIRASSEDAEVSDALGVDVRRVHLFVYGLAAACAAIGGVLVGITFSF
                     TPTTGLTYLLTGFAVVVLGGLGSVKGTLLGALLLGVIESVGGAFFGDGYRDFIGFVAF
                     LIVLSVRPQGLFGRQEAVRQ"
     gene            14374..15237
                     /locus_tag="VF995_03905"
                     /pseudo
     CDS             14374..15237
                     /locus_tag="VF995_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
     gene            15555..16961
                     /locus_tag="VF995_03910"
     CDS             15555..16961
                     /locus_tag="VF995_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9342745.1"
                     /translation="MLGVVGPNGAGKSTLIGLLSGAIAGDGQVELFGEDASGLGAQAR
                     ARRGIGRTHQVPRPFGQLTVLENLMVAQLHGARRGRRAARAECRRILARCGLLEYAGV
                     PAADLGLLRLKRLELARALAVHPRILLLDEIGAGLIEAELRELIALIQSLRDEVEAIL
                     IVEHVLDVIRECCDRLIVLDGGKLLVEGDPDQVLRDPQVAAVYLGTSGGGEVATLRDG
                     TAAGDGRRGGERPILEVKGIAAQYGHFRALHDVSLSVAAGEVLALLGANGSGKTTTAR
                     TINGMLPISAGEIWFDGQRVDGRAAHQIVRQGVAHCMEGRRIFADLTVEENLILGART
                     ANSKAELERRLGAVYELFEALREHRDNSGAALSGGQQQMLAIGRALMADPRLVIFDEI
                     SLGLAPIMVGRLYQALAEINGRGVAMIVIEQNVERGLALADHVAVLEKGRVALTGTPA
                     DVRSDDRLVSLYVGEARG"
     gene            17038..17235
                     /locus_tag="VF995_03915"
     CDS             17038..17235
                     /locus_tag="VF995_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342746.1"
                     /translation="MNVEASDMVTIPRAELDAMQAELRRLRRQVGRGVAMRRIQADPG
                     PGDEVPTFTRAELAGAWGIRE"
     gene            17228..17497
                     /locus_tag="VF995_03920"
     CDS             17228..17497
                     /locus_tag="VF995_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342747.1"
                     /translation="MSDRLVRFPGTSWDEIDALPQPLRWVVQRTIFHLLDEPAPTLAE
                     PFPDDDPLPGAYELHLPTDGVTIWYTVTMHEGQEVISVQHVSLDT"
     gene            complement(17561..18319)
                     /locus_tag="VF995_03925"
     CDS             complement(17561..18319)
                     /locus_tag="VF995_03925"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9342748.1"
                     /translation="MKDPRVVRKARAAAAAAAAPAAPRVPLSDAAYAEIRGRVVRCEL
                     APGQRFTEKQLAAELGFGVTPVREALARLDNEGLVVTMPRKGYQVAPLTIKVVDDLFG
                     VWRIVGPELVKLAVENMTQTQHQQIRQAFMKQRRIAKSRKLPPAAEWIGTAHESFLLL
                     AQATGNDRLVEIYQRLSNEMARVFTMVYVVSPPREWFPGGEQDYVGALDKRDAALMSS
                     LVRAFIERAHIEILKALWSWPSIRATEVVPPPAV"
     gene            18510..>19174
                     /locus_tag="VF995_03930"
     CDS             18510..>19174
                     /locus_tag="VF995_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9342749.1"
                     /translation="MRLTGKILWRGEPGYEEARLKPARAEMDWNARRVDRYPDAVVRA
                     TSDQDVVAAVRLARQRGLKVKARSGGHSWVDASIRDGGILVDLSQLDEVVVDPETRTA
                     WAGPGVKSQELFPHLKPHGLCFPSGFPSGGCRDVGLGGYVLQGGFGFNGGRIGLAAMN
                     VLAMDVVTAGGELVRADETTNPDLFWAARGSGSGFFGIVTRFHLRLHPLPGAVRTRAW
                     FFSA"
BASE COUNT         2845 a         6658 c         6941 g         2730 t
ORIGIN      
        1 taagcgtgct gcagcagggc ctggccgaac tcgtcggaga aggcggcggt gatctcggcg
       61 ggtgagaggc cctgggcggg gaggtcctgg ccgaggtaga agtggctgcg gagggcggcg
      121 ccccaggggg tgtcgcggac gatgtggagc aggcggccgc cgatgatcgc gccggagggg
      181 tcgcggtcgg gggtgtggct caggccgacg cggcccagga cggcgccgct gaccgcgccg
      241 ctggcacggg cggcggcgta ggcgccggcg tcgaagaact cgctgggctc gcggaactgg
      301 atggagagct tctcggcggg caggccggtg aactcctcga cgaccacgtg gatggagccg
      361 atatgcgtgc cttccctcgt ctcggtccag tcgctgaact ggtggtcgac cgggtgccac
      421 cagatgtact gctgggtgtc ggggcggaag cggaaccacc actcgaacat ctcgccgctg
      481 cagccgtgca ggtcggtccg ggcagcgacg tggaggaccc cgttgggaaa gcgttcgtag
      541 ccggtctcga tcgggagtgg ggtggggtcg agaagggcgc cgacctcgga aagctgcatg
      601 gatgttgcct cctctgctat tgatgcttgc cacgtgctcg cgcgcaatgc gggatgatac
      661 gtccaagtgg tggaactccg ggaggaccta tgagcgtcgt catcacggtc gcgccgaccg
      721 gcccaatcgc gaccaaagcc gacaacccct cgttgccgac cacgcccgag gagatcgcca
      781 aggccgccgg ggatgcttac agccttggtg cctccgtcgc ccacgtccat ctcagggacg
      841 agcaggagcg acccaccgcc gacctccgta tcggccgccg cgtgatggac atgatcgccg
      901 accggtgccc gatcctggtc cagctttcga ccggggtcgg cctgtcagtg ccgttcgagg
      961 agcgcgagca gctggtcgag ctgcggcccc gcatggcgac gctgaacccc tgctcgatga
     1021 gcttcggcga gggcgagttc cgcaacccgc ccaccggggt ccgccgcctc gccacccgca
     1081 tgcgggaact gggcgtcaag cccgagctgg agatctacga caccggccac ctggacgcct
     1141 gcctgcgact gcgagacgag gggctgctcg acgagccact gcagttcagc atcgtcctcg
     1201 gcgtccgcgg cggcatggcc gcgacccccg agaacctgct gacgatggtc cgccgcctcc
     1261 cagaaggagc catctggcag gtgatcgcgg tcgggcgggc gagcctcccg ctgaccgcgg
     1321 ttggccttgc aatcggcggc aacgcccgcg ccgggatgga ggacaccatc cacctcgcca
     1381 agggcaagct taccgacggc aacgacccgc tggtccagcg cgccgtcggc cttgctcacg
     1441 acctcggcat ggaggtggca ggcaccgatg ggacggagaa gctgttgcag ctcccccgac
     1501 ccatcgagcc ggccacctcc tgaccgctat gcggtggtgg tgaggtcgta gacggtggtg
     1561 ccgccgacgg tggtggattt gaaggtttgt tggacccagg aggtgatttg gctggaggtg
     1621 ctggagccgc cctggccgga gccgaggccg ctgccgccga tgaagtagtg gatcttgcct
     1681 tgggcgacgt attgcttgaa ttgggccagg gtgggggttg ggtcggtgcc gttgaagccg
     1741 ccgatggcca tgatcggttc gccggcggcg agctggacgc cggcggcgga gttggagccg
     1801 acggcagcgg cggtccagcg gtagccgctg gcgccctgct tcaggagcgc gaccagctgg
     1861 ctgctcgggg tgctgccgtt gagcaggctg ccgatgccac cacggccacc ctggcccccc
     1921 gggttcgcga agttgccggg gccgccggtg gtgccttggc cattggcgtt gccctggccg
     1981 ttgccgttgg tgcctccgaa ggggaagccg ccgggggcct ggccctggcc gttgccgaac
     2041 tgacggttgg tgccgaagtt gccgccacct ccggggcggc cgccgccgaa gccgaggcgg
     2101 ccggcgctgg cggggccgac cgacgggatc gcgccgctgt ggggctgtgc cgcggtggac
     2161 atcgagtagg cggctggggc ggcgagcgcg acgatcaggc cggctgccgc caccgccagg
     2221 gctgcccggc gggtgaagcg aaggctcggc atggcgagca gcagggcgcc gacggcgagg
     2281 ctgccgagca gcaccaccgg ggccagccac gggagccagc cggagacccg ctccagcagg
     2341 gtgaacgacc accagccggt ggcggccagg gaggcggcga gcaccgcccg cgccacgaag
     2401 tgggagcggt agcgccagaa gaaggccgcg ccgatgccga cgaccgcgcc gatcgcgggt
     2461 gccagcgcca cggtgtagta ggggtggatg atgccggcgg catagctgaa ggtcaggccg
     2521 gtgacgacca gccagccgcc ccacagcagc agggccgcgc gggttcggtc ggcccggggc
     2581 gtgcggcggg tcagccacag cccggcgccg agcaggatca gtgccgccgg gagcagccag
     2641 gtgacctcgc cgcccatctc gctgaagagg cggaacagcc cggtggtccc ccagcggccg
     2701 ccaccgccgg ccccgccgcc accgacactg ccggtctcgt tgccggtgag gcggccgaag
     2761 ccgttgtagc cgaaggtcag ctcaaggatg ctgttgtgct gcgacccgcc gatgtagggc
     2821 cggtcggcgg cggggatcat cgcgacgatc gccacccacc agccggcaga gacgaccatg
     2881 gcgaccccgg ccatcagcag ctggccgatc cgccgccgca gcggcgtcgg gcctgccacc
     2941 aggtagacca gcgcgaaggc gggcaccacc agcagcgcct gcagcatctt ggtgaggtag
     3001 ccgaagccga cgcagctggc ggccagcacc agccagctgg tgcggccgtt ctcgaccgcc
     3061 cgcaccatcg cgtaggcgcc ggcgacgagc agcagggtga gcagcgcgtc ggggttgttg
     3121 aagcggaaca tcagcaccgc gaccggcgtc agcgccatga ccgcgccggc cagcagcgcg
     3181 gcaggggccg agaaccagcg ccgcaccgcg gcgtacagga tcccgacggt ggcgacgcct
     3241 tcgagggctt ggggaaccag caggctccag gagttgacgc cgaagatacg cgccgagatg
     3301 tccatgaccc acagcgaggc cggggtcttg tccacggtga tgaagttgga cgagtcggag
     3361 gaaccgaaga agaacgcctt ccagctcttg ctcccggcct ggacggcggc ggcgtagaag
     3421 gtgttggcgt acccggaggc gccaagcgac cacaggtaca gcagcgcggt cgcggccagc
     3481 agcgccagca gcgccgggcg gacccagggc tggtcgctct cccgcccgcg caccaggcgc
     3541 cgcagcaggc cggtgcgcct ggagccgctg gcgggagcgg cactccccca ggcggtcggc
     3601 ttgtcgatgg tggtagtcat gggtggatcc tttgcatcga tggagttggc ggcggagtca
     3661 gtggcggcgg tgctgtcgtt gtcggtgtgg ttgggaacac ccagctgcgg aacagcacga
     3721 accgcagcag ggtggcggcg aggttggcgg cgaccagcac gccaagctcc agcatgcggg
     3781 cgggcgtcct ggtcacggca tgcaggatcg ccagggtgct gctggtcagc accaagccga
     3841 gcgcgaacac ggccaggccc tggaggtggt ggcgcccggc gcgggcgacg ccgcggacgg
     3901 cgaaggtgac gcgccggttg accgcggtgt tgcccacggc ggtcagcagc aacgcgaggg
     3961 cattggcgac ctgcgcgccc atccagtcgt gcagcagcac gaacagcacc aggtaggcaa
     4021 gcgtgcttgc caccccgacc acggcgaagc gcagcaactg ccgggcgagg ccgctaggca
     4081 cccccggcag cggatccggg gcgccctcgg ggccgtgggc ggtggccgtc tggcggaggg
     4141 tacgcaccgg gatcttcccg ctggcaagcc cccaggccag gcgccgcacg ccgcgcaggt
     4201 cggcgagggc ggtcgcgacg atgtccacgc gggagtccgg gtcgtcgacc cagtccaccg
     4261 gcacctcgta gatccgcagc cccgcccgct cggcgaggac cagcagctcg gtgtcgaaga
     4321 accaggccgt gtcctccacc aatggcagca actcgcgggc ccggtcggcg cggatggcct
     4381 tgaagccgca ctgcgcatcg gagaaccggg cccgcagggt ggtgcgcagc agcaggttgt
     4441 agcagcggga gatgatctcg cgcttgggcc cgcggaccac ccgcgacgac gcgttcagcc
     4501 gggtgccgat ggcgacgtcg ctgtggcccg acagcagcgg ggccaccagc ggcagcagcg
     4561 cggacaggtc ggtcgacaga tccacgtcca tgtaggccaa aacggtggcg tcgcttgccc
     4621 cccacacggc ccgcagcgcc cgcccccgcc ccttctcgtc caggtggacg gcgcggacgc
     4681 cggggagcga ggcgctcagc acgcgggcga tcggccaggt ctggtcgctg ctggcgttgt
     4741 cggcgatggt gatgcggaag ctgaacggga acgactcggc caggtaggtg tgcagccggg
     4801 tgacgctgcg ttcgaggtcg cgctcctcgt tgtagaccgg gatgacgacc tcgacggcgg
     4861 gggctgcaga gggggcggcg gacgcccctt gcgacacggc cgctccggcc gcgagggggg
     4921 ccgcaagggg cgccgccatc tgggaccggc ggtcggcttt gccttcgcct tcgccggtca
     4981 cggtgtgctc cgtcgtcctg gtcatggcgc cacagtgacc gccctagctg gggggcggct
     5041 gaagccaacc tgtgagttcg ctggaaagcg gagcgagccg gaaataccgt gtatgaacgc
     5101 cttataggtg ccggctacac tcgctcggcg ccgacgtgga agaccgccag ggtcgctggt
     5161 ctgtcggtgt ggttccgcca gccgtggcga gtgccgttct ggatgaccac gtcaccttgg
     5221 cgaagatgcg tgagggcgcc gtcgtccaac tccagccaga gctcgccgtc gaggacaatc
     5281 tggtagtcga cggtcggggt ccagtgcatg cccgggttgt caggctcgaa ccgatcggcg
     5341 atccctggag tggcgaggcg cccctcggcg tcggccgcca ccgggtcgaa gcccgggtcg
     5401 gcgtagacgg agtccggcgg gaaggtcacg atgacgacgc aggtgccgcc gggctcgggc
     5461 acctgggagc ggaccgccgc ggtggggtcg gcgccgtccc ggctgatcgg ctcgcccggc
     5521 tcggtcgccc acagcatggt ggtggccatc atcgggatgt gcacgaagtc gtggctgcgg
     5581 ggagcgggcc cgtcgctgac gacgacggct ttcccctcag cagtgtgacc ggtgaccacg
     5641 cgacgtatct gcaacggata ctcctttcag ttccatcagt cccatacggc ggcgaatgcc
     5701 gccagttccc gtgggatgtc cggtcccata gggccgtaca gcaccccctc cacgccggag
     5761 gcggcaatct cgtcgaggcg tcggcgcacc tcgcccggcg tgccggtgcc ggtcgtcccg
     5821 gcgatcagcg tgccggtgac cagcgggcgc tccaggtcgg tcacctcaat caggtggccg
     5881 cggtggacct ccaggtggcg gcggcgcggg tcgagccgcg cgacggcctg gtggaacgcg
     5941 gccgactcgg gcgcttcggc cacgatgccg tggtcgccga tgtgcagctg gaaggcggcg
     6001 taggggccgg cggcgtgcag cacccgctcg ctgccggggt cctcgccctc ctccagcacc
     6061 gtgccgtagt acaggacctg cacgcggccc atgtccggcg gcagcgggtt gctcccgcgg
     6121 tggctcaggt tggtgatgat gccgtcaccg acgcgctcag cggcggcgta gccgcgcggc
     6181 ccgtgcgcgg cgatgcggat ggggaggtca gccggcagcc gcaccccggc gcgggtgccg
     6241 tagcgcagac cgatgaccga cccgtcccat tcgacctcct cgccccgcag cagcgcccgc
     6301 agggcaatgg tgtaggcctc cacctccgcc cagcgggcgg gcggcttgcc cagcatgagc
     6361 tggctggtga agcccgcgcc aacgcccacc tcgatgcggc cgggcgccag cgtgctgagg
     6421 gtcgccagcg ccccggcgct ggcgacgacg tggcgcaggc gcggggtgat cacccccacg
     6481 ccgaggccga tccgcgaggt cctggcagcc gccagggcga gcgtcaccca ggggtcggcc
     6541 aggaacgccg gcgagtcgta gaccagcgcg cggcggtacc cgagcgcctc cgcccgctcg
     6601 atgtgggcgg gcgtctccat cgtcggggga aacaggcagc cgagttccat ccttcgggct
     6661 cctcaacctc gatttcgcca gtgtggccag ccctgggcat gccttgacaa gccatcaggg
     6721 cgcctcctaa tgtctcgttc tgcggaccag ggtgaccgca tcctggaaca ttatcttcgg
     6781 gtacgcaacg tcttcaatcg caaggactgg agaggagcca acggtggtcg ttgaacccgc
     6841 cacgggactc gtcttcgtcg agctgagcca cgtttggggc gctcacaccc cggcattccc
     6901 gggttataag gacatccagg tccaccggct ctccactcac gcacagtacg gcgtcctgac
     6961 ccagcgcatc gtcacagtga tgcaccacgg cacccacgtg aacgcccccc tgcacctggt
     7021 ggctgggggg gaaagcgtcg gcgacctgcc gctcgaccgc tttttcggca acggcgtggt
     7081 gctgccggtc cccaagggcg aatgggagct gatcgagcct gacgacctgg aggccgccca
     7141 ggcggccagc ggcgagcagg tccagccggg cgacatcgtc ctgctcaaca ccggctggca
     7201 tcacggctgg tccgacagca tggagtactt cggccacagc cccggcctca gcgaggcggc
     7261 ggcgcagtgg ctggccgacc accaggtcaa gctcgccggc atcgacaccg ccaccgtcga
     7321 ccaccccatg gccacctcac tggcccgcaa ctaccggggc gtcggcccaa tcgtcaggga
     7381 gttgccgcgc cgctggcgcg cccgcaccgg gcacgagccg caggaggact tccccgcgtg
     7441 gatgcccgcc caccggctcc tgctcggcgc cggcatcccc accatcgaga acgtcggcgg
     7501 ggacctcgac agcgtcaccg gcatgcgctg cgccttccac gccatcccgt ggtattggcc
     7561 agagggtgac gcctgcgtga tccgcctggt ggccatcctc gacccaaccg gcgaataccg
     7621 gctggagaaa ggttcctgag ctgatggctg gcaccccttc accttcaacc agcaccgacg
     7681 gcctgcggtt ctacagcctc agccacccgc tgggccacaa catgccgcag tggccctccc
     7741 ccatggccac gccgctggtc accagcaggc tctactacca cgccatgcac ggcttctgta
     7801 cctacgagta cgacggcatc atgcaccgcg ggacccacat ggacgtcccg gtgcacgtga
     7861 ccgccaatac gccctacctg ctcgacttcc cgctgtggcg cttcttcggc accggcgtgg
     7921 ccatctcggt cccgaagggc aagtggggcg tggtcacccc cgacgacctg gagcgggccg
     7981 agcccaagat ccagccgggc gacatcgtca tgatcaacac cggcttccac cactacttcg
     8041 gcgacaacga ccgctactac gcctactccg gcggcctcta caaggaggcg gcgcagtggc
     8101 tggtcgacaa gcaggtcaag ctggtcggca tcgacgtgca ggcgctcgac cacgccctcg
     8161 gcacccgcat ggtggagcac ggccccggcc cgacccaccc gcacctgatg gaggagtacc
     8221 gcgccgagac cggccgggac gtgatgatgg acttccccga ctgggagccc gcccacaaga
     8281 ccatgctgct ggccgggatc cccggggtgg agaacgtcgg cggcgacctg gatgcggtga
     8341 ccggcaagcg ctgcaccttc atggtgttcc cgtggcgctg ggccaacggc gacggctgct
     8401 cggtccgggt ggtcgcggtc atcgacccgg agcagaagtt ccgcatcccg acgggaaccc
     8461 cggtgggagc ctgagatggt ggctgacgac gcgctccaca accccctgtc gctgttcgac
     8521 gtctccggca aggtcacggt gatcaccggc gcctcgggtg cgctcggcca cgcggtcgcg
     8581 gtcgccctcg ccgcgctcgg cagcaagctg ctgctggtct ccggctccga acaggccctg
     8641 gaggcggtcg ccgccgaagc ccgggagacc gccgcagcgc gcaactccgg cggcgaggtc
     8701 cagaccctgg tgcgccggcc cgagaccctg gaggacgccg aggtcattat cagtacggcg
     8761 gtggcggcgt tcggccaggt ggactcgatg ctggtcgcct ccggcctcaa ccagccgggc
     8821 ccgatcgagg agcagcccta cgaggactgg caggccgtca tggacgccaa cgtccgcggc
     8881 ccctggctgc tggccaaggt cttcggcccg cacgtgttcg cccggggcgg caagggcaag
     8941 ctggtgctga tctcctcggt gcgcggccgc cacggccacc cggtcggcta cagcgcctac
     9001 tgcacctcca agggcgccac cgacgccctc accaggacgc tggcggtcga gtgggcaacg
     9061 cgcgggatca acgtcaacgc catcgccccg gcggtgttcc gctccaccct caccgactgg
     9121 atcttcgccg acaccaagct gggcgaggag gcgcgggcgc gcaactactc ccgcatcccg
     9181 atgaagcggc tcggcgagcc gcaggacttc atcggcatcg ccctctacct gctgtccgat
     9241 gcctccgact gggtcaccgg gcagatcatc tacgtggacg gagggtacac agcagcatga
     9301 ccggtgagcc gagggtctcc attgtgggtg cggggttgat gggcgcgggc atcgcccagg
     9361 tgttcgtggc ggccggcctg gaggtcagca tttacgaccc ggcgccgtcg gcgctggcct
     9421 cggcgccgga ccgggtccgg gcggggctgg agctgctcga tccagagcgg gacagcggcg
     9481 aggccctgcg gcggctcgcc ctcaacacgg atctggcggc cctggtggag ggcgcggacc
     9541 tggtgatcga ggcgggcccc gagcgcgtgg aagtcaaacg ggccatcttc gcagacctgg
     9601 accggctcgc ccccgccagc gcggtgctga cgagcaacac ctccgccatc cccatcaaag
     9661 agatcgcgcg agactgcgag cagcccgagc gggtcatcgg cacccacttc tggaacccgc
     9721 cggcgctggt cccgctggtc gaggtggtgc agtcagaggc aagcgaacca accctggtcg
     9781 agtggaccat ccggctgctc ggccaggtgg gcatgaaacc ggtgcacgtc aggagcgata
     9841 cgccgggctt tgtcggcaac cgcatgcagc acgccctgaa gcgcgaggcg atcgcgctgg
     9901 tcgccgaggg tgtctgcgac gccgagacgg tggacgtcgt ggcccggttc gggttcgggg
     9961 cgcggcttgg catcctcggc cccctggagc agtcggacct ggtcgggctt gggttgcttc
    10021 tggacatcca caagacgctg atgccagcct tgaatcgcac ggcggtcccc catccctacc
    10081 tggtggagaa ggtccgccgg ggcgagaccg gcgcggcggt gggtcacggc ttccgcgagt
    10141 ggacgcccga ggaagcggcc gcgcgccagg cggaggtgac ccgcgagctg gtcgcggcgg
    10201 cccggcggcg ccgggaggcc gaggcgcagc agtaaccgca ggaactatgc acggagcacg
    10261 acgagcaacc tggcgaaccg acggaggagg tcgacgatga ctcggctggc gggaaaggta
    10321 gcgctggtca ccggtgccgg cggcggcatc ggccgggcgg cggcggtgcg cttcgcggag
    10381 gagggcgcgc gggtcggcat caccgaccag gacgccgcca acctggagga gaccgccgcc
    10441 gcagtcaaag ccgaattcgc gaaggtcgcc ggcaccggcg acagtggtgc aggcgatggc
    10501 gtcgtgcagg tcgtcgggga catcgtcgac ccggccacga tcgaccaact ggccacgcag
    10561 acggtggagc ggttcggcca gatcgacgtg ctgttcaaca acgtcggcat cctgatcctg
    10621 aagtcgctgg ccgacaccac ccctgaggac ttcgaccggc tgatgcacgt caacgtcctg
    10681 tcccacctgc tggggatcca gcgggtcgcg ccggagatgc ggcgcgcggg cggcggctcg
    10741 atcatcaacg tcgcctccgt cggcggcctg gtcgccctgc cgaacgtgag cgcctactcg
    10801 gcctcgaagt cggcggtgat cggcctgacc cgctcggccg cggtcgagtt cggccccgag
    10861 atccgcgtca acgcggtctg ccccggcggc gtggacaccc cgatggcgca acgccacctc
    10921 gccagcttcg aggacaagca agcggcaatg aagctgctca ccggccgcca gatcatcaag
    10981 cgctacgccg agccgcgcga gatcgccgac gtggtggtgt tcctggccag cgacgaggcc
    11041 tccttcgtca ccgggacggc catcgccgcc gacgccggcc acaccacctg gtaagaggaa
    11101 ggccccaaca gtggagttgg cagaggcgaa ccaagtggtg acggcgcccg agtggggcga
    11161 ccggatcgag gtgctgtcac caacccgggt ccgggttgcc gcgctggacc ggctcggggg
    11221 gatcacgcca gcgatgatcg actggtggtt cggccggatg gacgaggaga catatttcag
    11281 gttccatccg gtggaccatc agaaattcgc gtgggtgcgt ggcaaacagc cgggcagcta
    11341 catcggcgcg acgcacctca cctaccactg ctacggaggg ctcggcccgg tgcttcgcag
    11401 ccacatcacc ttcatcccac caagggagct actcgatgag ggcttattcg aggcgcacgg
    11461 ggtgggcgcg gcggtctgcg cggtggtgca cctgctggac gagcacgacc ggcccgaaca
    11521 ggaggaggcc ggacgcttca tgcacgtcgc ggtcgagcgg gggtatggga ccgagttgcg
    11581 gagctgctgg tggctgacgg tcgggcccga cagcgacgtg gagctgctga ccaccgggcg
    11641 gctgcggcat gtgcacgagg agttcgccta cctgacaggc ttcctgcctg gactctataa
    11701 ggaggaggtg ggccagcgat gacgctgcgg ttcgcggcca acctgtcgat tctgtggacc
    11761 gaccggccct tcgcggagcg gttcgagcgg gcggccgccg ccgggttcga cacggtcgaa
    11821 ctgtggtggc cggggttgga ggcggccgag gagctgcccg aactcaccgc tcgcaaccgg
    11881 ctcgggctgg cgctgctcaa cttcgacggc ggggacatgg cggccggcga gcgcgggctg
    11941 gccaacgacc cggagcgcca cgagcagctc cgcgccaaca tcccggcagc gctccggatc
    12001 gctgaggcct gtggctgcac gcggctgaac ctgctggtcg gccagcagca cgaccgcgcc
    12061 accctggaac agcagcacgg ctgggcggcc ggcgaggtcg cctgggcggc ggatcaggcc
    12121 gccccgcgcg gctgcgaggt gctgatcgag gcgatcaact ccttcgagaa cggcccctac
    12181 ctgctcacca ccaccgcggc ggcggcggac ttcgcgcagc gggtcgggcg gcccaacgtc
    12241 cgcctccagt acgacgccta ccacatgcag cgcatggagg ggaacctcac ggcgaccatc
    12301 gacacgtact ggccgctggt cagccacatc cagatcgccg acgcgcccgg ccgcaacgag
    12361 ccgggcaccg gcgagattaa ctaccggtac gtcctcgatc acatcgagcg caagggctac
    12421 gacggcttcg tcgggctgga gtaccggcct tccaccggga acgccgacga ctccttcggc
    12481 tggctcgaca cgctcggacg accgcgtggg agcagaacat gagcagcaac agcagcggga
    12541 ccgtcggctt catcggcctc ggcgtcatgg gccgcccgat ggcggagaac ctcctccgcg
    12601 ccggggtcga cctcgtcgtc tggaaccgca gccaggagcc gcaggaggcg ctggtcgcgc
    12661 aaggcgcgac cggggtcgtc accatccagg acgtcggcaa gcgggccgac gtgatcatca
    12721 ccatgctggc cgacgatctg gcagtccgcg acgtgatcct gcaccgcctg gtgctgctgc
    12781 cagccgccag gcccggatcg ctgattatcg acatgagcac ggtctcgccc gcgatcagcc
    12841 gcgagatcgc cgagcgcgcg gccggcaagg gcatccacac gctcgacgcc ccagtcagcg
    12901 gcggcgacgt cggcgcgcgg gagggcaccc tctcgatcat ggtcgggggg gaggccgccg
    12961 acctggagcg agcccggccc ttgctggaga tcctcggcgg cagcatcgtc cacgtcgggc
    13021 cggcaggagc cggccaggtg gtcaaggcct gcaaccagct gctggtggcg atcaccatcg
    13081 cgggggtgag cgaggcgctg gtgctcggct ccaagctcgg cgtcgcgccc ggcgccatcc
    13141 tcgaggtgct gtcgcggggg ctggcgggca accgcgtgat ggagctccgc cgcgacaact
    13201 tcctggagca ccgcttcacc cccggcttca aagtcgacct gcaccacaag gacctggaca
    13261 tcgccctggc cagcggcggc caggccaacg tccccctggc cctcaccgcc gccgcgcagc
    13321 agctcctgcg ccagctccgc gcagcagggc gtggcggcga ggaccactcc gccatcctcg
    13381 ccgccgtcga ggcccaagcc gcgcaccgca tcggcacccc gaccgccaca ggcgccacag
    13441 aggagcaagg ggagtcgcca tgaccatccc gaccggctcc gcgttcatca acgcgctggt
    13501 ctccggcctg ctgctcggcg ggctgttcgc ggtcaccgcg ctgggcctgt cgctggtctt
    13561 cggcgtgatg cgcctgatca acctggtcca tggggagctg atcgtgtttg gcgcctacct
    13621 ggcgctggag ctgacccgcc acagccacgt cacgcccctg ctgaccatcg tggtggtggc
    13681 cccggtgctg ttcctggtcg ccgcgccggt gcagcggctg ctgctggagc cgctgatggc
    13741 caagggcccg gagccggcgc tgctgaccac cttcgggctc tcggtgatcg cccagaacct
    13801 gttcgtgctg gtctggtcgg gtgacgtgca gtccctgccg gcccgttacg ccaccggtgc
    13861 ggtgaaggtc ctggggctgc ggatcgccga catgtatctg atttcgttcg cggtggcgct
    13921 ggtgctgtgc gggctgctcc acctgatgct gcagcgaacc cgcctcggcc tgcggatccg
    13981 cgccagctcc gaggacgccg aggtctccga cgcgctcggc gtggacgtgc gccgcgtgca
    14041 cctgttcgtc tacgggctgg cggcggcctg cgcggcgatc ggcggggtgc tggtcgggat
    14101 caccttcagc ttcaccccga cgaccggcct cacctacctc ttgaccgggt tcgcggtggt
    14161 ggtgctcggc gggctcgggt cggtcaaggg caccctgctc ggcgcgctgc tgctgggcgt
    14221 gatcgagagc gtcggcgggg cgttcttcgg cgacggctac cgcgacttca tcggctttgt
    14281 cgccttcctg atcgtgctgt cggtccggcc gcaaggcctc ttcgggcggc aggaggcggt
    14341 gcggcaatga aggcggtcaa agcgacgcgg gggatgcgca cgagcgggct ggtcacgccc
    14401 cgccggatcg tgccgctggt gctctgcgcc gcggtgctgg tggcgttcat ctcggcgccg
    14461 cagtgggcca gccagtacca gctgctggtc ggcttcgagg tcctccagct cgccgcgctc
    14521 gcacaggcgt ggagcatgct ggccggctac ggcggtgtgg tgtcgctggc ggtgtcggcc
    14581 ttcgtcgggg tcggcgccta cgcgaccgcc aagctggcgg tcgccgccgg gctcgggatc
    14641 ctgccgtcga tccttgccgg cgggctgttc gcggtggtgt tcgcggtgat cgtggcggtg
    14701 ccgctgctgc gcttccgcgg gctgtacttc accatcgcct cgctggtgct ggcgcaggcg
    14761 ctggcgatct tcatgtccaa ctacaacggc ctcggcggca accacggcat caccctgctg
    14821 agcgccgcgc cgacccaggc ccaggtctac aacttctccc tggtggtcgc gatcgcggcg
    14881 acgctggcgg cctggtggat cgtgcgcagc cgcctcggcc tcgggctgat ggcgatccgc
    14941 gacgacgagg aggtggccga acgggtcggc gtcgccacct tccgcaccaa gctggtcggg
    15001 ttcgccctgg cggcgctggt gatgggcctg gtcggcggga tccaggcaca gcgggtcggc
    15061 tacatcgagc cgctgggcgc cttcaacctc aactggacca tcgagtcgat caacgccgcg
    15121 atcatcggcg gcgtcggcac catcgccggg ccgctgctcg gctcggccgc ttcgatcgcg
    15181 ctctctgaga agctcgccaa ctaccccgag ctgcatctca tcttcgtcgg ggtcatcctc
    15241 atcgtgatca tccgcctggc ccccgccggc ctgtggggca ccgcgctgcg gctcgcacgc
    15301 cgctggcggc agggcgcggc ggcacccgcc cgtgcggcgg cggccggggc cgtggccgga
    15361 gccgtggccg gggcgcaggc ggtcgtgacg ccggcggcag ccgccaatcc cggagggaac
    15421 gcggacgccg ccgacggcga cgcgacggcg acggcacgac ccgcacgccc ctcctgcggg
    15481 ccgccagggt cggcaagcgc tacggcggcg tccacgcggt caacggcgtc gacctgcaga
    15541 tccgcgccgg ggaggtgctt ggcgtcgtcg gccccaacgg cgccggcaag agcaccctca
    15601 tcggcctgct ctccggcgcg atcgccgggg acggccaggt cgagctgttc ggcgaggacg
    15661 ccagcggcct cggcgcccag gcccgcgcgc ggcgcgggat cggccgcacc caccaggtcc
    15721 cgcggccgtt cgggcagctc accgtgctgg agaacctcat ggtcgcccaa ctgcacggcg
    15781 cccggcgggg acggcgggcc gcacgcgccg agtgccgccg catcctggcg cgctgcggcc
    15841 tgctggagta cgcgggcgtg cccgccgccg acctcggcct gctgcgcctc aagcgcctgg
    15901 agctggcccg ggcgctcgcc gtgcacccgc gcatcctgct gctcgacgag atcggcgccg
    15961 gcctgatcga ggcggagctg cgcgagctga tcgccctcat ccagtcgctg cgcgacgagg
    16021 tcgaggcgat cctgatcgtc gagcacgtcc tcgacgtgat ccgggagtgc tgcgaccgcc
    16081 tgatcgtcct cgacggcggc aagctgctgg tggagggcga ccccgaccag gtgctgcgcg
    16141 acccgcaggt ggcagccgtc tacctcggca cctccggcgg cggcgaggtc gcgacgctgc
    16201 gcgacggcac cgccgccggc gacggccgcc gcggcggcga gcgcccgatc cttgaggtca
    16261 agggcatcgc cgcccagtac gggcacttcc gggccctcca cgacgtctcc ctctcggtcg
    16321 cggccggcga ggtgctggcg ttgctgggtg ccaacggcag cggcaagacc accaccgccc
    16381 ggacgatcaa cgggatgctg ccgatcagcg ccggggagat ctggttcgac ggccagcggg
    16441 tcgacggccg cgccgcccac cagatcgtcc gccagggcgt ggcccactgc atggagggcc
    16501 gccgcatctt cgccgacctc accgtcgagg agaacctgat cctcggcgcc cgcaccgcca
    16561 actcaaaggc cgagctggag cgccgcctgg gcgccgtcta cgagctgttc gaggcgctgc
    16621 gcgagcaccg cgacaactcc ggcgcggcgc tgtccggcgg ccagcagcag atgctcgcca
    16681 tcggccgcgc gctgatggcc gacccgcgcc tggtgatctt cgacgagatc tccctcggcc
    16741 tcgcgccgat catggtcggc cgcctctacc aggcgctcgc cgagatcaat gggcgcggcg
    16801 tcgccatgat cgtgatcgag cagaacgtcg agcgcggcct cgccctcgcc gaccacgtcg
    16861 ccgtcctgga aaagggccgg gtcgccctca ccggcacccc cgccgacgtc cgcagcgacg
    16921 accgcctcgt cagcctctac gtcggcgagg cccgcggctg acctcagcaa agaacagccg
    16981 tcggggcctc atcccgctag actttcagcc gagagcgctg agccgaggga gccgcgtgtg
    17041 aacgtcgaag catccgacat ggtcaccatc ccccgcgccg agctggatgc gatgcaagct
    17101 gaactgaggc gcctgcgacg ccaggtcggg cgaggcgtgg ccatgcgacg cattcaggcc
    17161 gatcccggcc ccggcgacga ggtacccacg ttcacccgtg cggagctcgc cggagcctgg
    17221 ggaatccgtg agtgaccgcc tcgtccgctt ccccggcacc tcctgggacg agatcgacgc
    17281 cctgccgcaa cccttgcggt gggtagttca acgcacgatc ttccatctgc ttgacgagcc
    17341 ggccccaacc ctcgccgagc cgtttcctga tgacgatccg ctgcccggcg catacgaact
    17401 gcacttgcct accgatggtg tgacgatctg gtacaccgtc acgatgcacg aaggccaaga
    17461 agtcatcagc gtgcaacacg tcagcctcga cacctgaccc gaccgcccgg agagcgcgcc
    17521 gcagcggagc tcggctagca cgcgaacgcg gaacgctcac tcagacggcg ggtgggggga
    17581 cgacttcggt ggcgcggatg gagggccagc tccagagggc cttgaggatt tcgatgtggg
    17641 cgcgttcgat gaaggcgcgg accagggagg acatcagggc ggcgtcgcgt ttgtcgaggg
    17701 cgccgacgta gtcctgttcg ccgccgggga accattcgcg gggcggggag accacgtaga
    17761 ccatggtgaa gacgcgggcc atctcgttgg agaggcgctg gtagatctcg accaggcgat
    17821 cgttgccggt ggcctgggcg agcagcagga agctctcgtg ggcggtgccg atccactcgg
    17881 cggcgggggg aagcttgcgg ctcttggcga tccggcgctg cttcatgaag gcctggcgaa
    17941 tctgctggtg ctgggtctgg gtcatgttct cgacggcgag cttgaccagc tcggggccga
    18001 cgatgcgcca gacgccgaag aggtcgtcga ccactttgat cgtcagcggg gcgacctggt
    18061 agcccttgcg cggcatggtg acgaccagac cctcgttgtc gaggcgggcg agggcctcgc
    18121 ggacgggagt cacgccgaaa ccgagctcgg cggcgagctg cttctcggtg aagcgctgcc
    18181 cgggggccag ctcgcagcgg acgacgcggc cgcggatctc ggcgtaggcg gcgtcggaga
    18241 gcgggacccg cggcgctgcc ggggcggcgg ctgctgctgc tgcagcgcgg gccttgcgga
    18301 ccactcgcgg atctttcatg tacgagtcga tctctggcac cgctggcact ggcgccggct
    18361 tcgagcgggc gggactcatt ggcagcatct cctcatacgg cgcattgttc ccgggcgacg
    18421 gcagcggcgc aaccgccgcc ggactggaac tcgggcatct gatatatcat cagctccaac
    18481 tttagacctc acagacctgg cggtcagcaa tgcggctcac gggcaagatc ctgtggcgtg
    18541 gcgagcctgg ctacgaggag gcccggctga agccagcacg ggccgagatg gactggaacg
    18601 cccgccgcgt cgaccgctac ccggacgcgg tggtgcgggc caccagcgac caggacgtgg
    18661 tcgcggcggt gcggctcgcc cgccagcggg gcctcaaggt caaggcgcgc tccggcggcc
    18721 acagctgggt ggacgcgagc atccgcgacg gcggcatcct ggtcgacctc tcccagctgg
    18781 acgaggtggt ggtcgacccg gagacccgca ccgcctgggc cgggccgggg gtcaagagcc
    18841 aggagctgtt cccccacctc aagccgcacg gcctctgctt cccctctggt ttcccctctg
    18901 gcggctgccg cgacgtcggc cttggcggtt acgtcctgca gggcggcttc ggcttcaacg
    18961 gcggccgcat cggcctcgcc gccatgaacg tgctcgccat ggacgtggtc accgccggcg
    19021 gcgagctggt tcgcgccgac gagaccacca accccgacct gttctgggcg gcgcgcggct
    19081 ccggctccgg cttcttcggg atcgtgaccc gcttccacct gcgcctgcac ccgctccccg
    19141 gcgcggtgcg gacccgcgcc tggttcttca gcgc
//
LOCUS       DASWDC010000125        12898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1517948, whole genome shotgun sequence.
ACCESSION   DASWDC010000125 DASWDC010000000
VERSION     DASWDC010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517948"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(475..810)
                     /locus_tag="VF995_03935"
     CDS             complement(475..810)
                     /locus_tag="VF995_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342750.1"
                     /translation="MTSGVSPGIDRNARSSAASAGAGVAALTGAAPAGRASGLPPRSP
                     PMAPPLKRRTARPATTSPPAPTAARRNSRRDRPSPRAGGGTTSRSSRPSAPPSASANT
                     APAAPTKAA"
     gene            1158..1676
                     /locus_tag="VF995_03940"
     CDS             1158..1676
                     /locus_tag="VF995_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9342751.1"
                     /translation="MAYGMNGQSLPRAHGYPARIVAPGIYGMKCVKWLQRIEVVNYDY
                     QGYWQRGAGWDNIATIKSASRIDVPHDLAGVKGELMVAGLAWAGDRGIDRVEVSFDGG
                     TTWTPAILRRELARAAWRQWRLPWRPSGAGKTVLKVRAVDGQGQPQSAGVAPPYPSGA
                     TGYQQIEVITST"
     gene            2004..2639
                     /locus_tag="VF995_03945"
     CDS             2004..2639
                     /locus_tag="VF995_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9342752.1"
                     /translation="MAADGSSGTRAPADETPEERERRFADEAIPLLDTIYAGALRLTR
                     NPADAEDLVQETFLRAYNAFHQFEPGTNLKAWLYRILTNTYINSYRKQQREPQSVPTE
                     DVDDFSLYQHIANANPAGAESPEEVLLDQIPEDEVRQALDSLPEQFRVAVLLADVEGF
                     SYKEIADIMGTSIGTVMSRLHRGRRQLQKRLWDFAAGRGLLEEGQEWVRPK"
     gene            2621..2893
                     /locus_tag="VF995_03950"
     CDS             2621..2893
                     /locus_tag="VF995_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX9342753.1"
                     /translation="MGAPEVGDCGCDGVLNHVWSYLDHESDPPTCAELEAHLRTCTYC
                     QGLVQFNEQLKKLVRRCADPGPAPAAASAQLRARVQAIIKVRRTLS"
     gene            3012..3212
                     /locus_tag="VF995_03955"
     CDS             3012..3212
                     /locus_tag="VF995_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342754.1"
                     /translation="MRSENVKGHRPGNDPAPTKVERVGRVCREADCETRLSIYNKTGF
                     CWQHQPLIFPNYRGKRLANSGS"
     gene            complement(3313..3714)
                     /gene="sodN"
                     /locus_tag="VF995_03960"
     CDS             complement(3313..3714)
                     /gene="sodN"
                     /locus_tag="VF995_03960"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEX9342755.1"
                     /translation="MGKRLATWLQPTRVAHAHCDLPCGVYDPAQARIEAESVAKIIEK
                     YHQSNDQDFRTRALIIKEQRAELVKHHLWVLWTDYFKPPHLEQFPNLHALFWQATKQA
                     GEAKHSTDPAVAQKLLEQIDEIAGIFQKTKS"
     gene            3833..4297
                     /locus_tag="VF995_03965"
     CDS             3833..4297
                     /locus_tag="VF995_03965"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX9342756.1"
                     /translation="MNIRRAVPAVVVLIGAAGLLTLTLRRTRLEPMLVDGDSMRPTLA
                     PGQRIAVAPLTGPPPRGALVVLRRPASQGPQGSQVEGERLEVVKRVAALPGDRVWLAG
                     TETTLAADEYLVLGDNPARSIDGRSFGPVHSDDFVGIVRFAYWPPHRLARRG"
     gene            4370..5320
                     /locus_tag="VF995_03970"
     CDS             4370..5320
                     /locus_tag="VF995_03970"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9342757.1"
                     /translation="MLVVVAGGAGFLGANLVDRLQAEGDEVVVVDDLSHGHLANLTEA
                     RRRGGVRFHRMDIAQGGLATLAARIRADAWVHLATPTDPVRAWTDASTDGRLLVLGAT
                     ELLQVAAASGARVVLASSGAYLFPNQLTGVTTADDQPAPTHPHGAGRLAVEAYANAFA
                     ARGLEIAVLALGTLYGPRQDPQRPGLVARAAWSMLQGKAPRVEGDGRQCRDFLFVDDA
                     VDALARAVHTDVRGRLLLGTGSATSVLEVIEQLASLAGWAGEPEWAPARPGDPRRCAL
                     DPNPAGHALGWQPWTRLEEGLAMTVEWLKARLPADHRYTE"
     gene            5604..5792
                     /locus_tag="VF995_03975"
     CDS             5604..5792
                     /locus_tag="VF995_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9342758.1"
                     /translation="MEAPSYLRTAEVADILHVSPKTVSRWAKEGKLPFLKTLGGHRRY
                     PEAKIQELAEELREEPTG"
     gene            complement(5883..6503)
                     /locus_tag="VF995_03980"
     CDS             complement(5883..6503)
                     /locus_tag="VF995_03980"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HEX9342759.1"
                     /translation="MGLLLLLLFVAVAGIGIGVVRGGQPKGLAEASVRGKPLGLICLM
                     LQGVIGALALRSIGLPRGVGVLLLALTLVVLLGVARANGQLPGVPLLALGLLCNLLVL
                     ILNLGVPVAATTLEHAGITVERPLAHRPDAKHVLEGGGTRLAALGDQLAVRPLHAVVS
                     VGDVAELAGIFLLLQGMVLATPSRRRADAAHGAHEARTVPWSTWAD"
     gene            complement(6607..7254)
                     /locus_tag="VF995_03985"
     CDS             complement(6607..7254)
                     /locus_tag="VF995_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9342760.1"
                     /translation="MDDGVVRILVADDHPLTRDGIKAALSLDETLKVVGEARDGDEVV
                     RLAAELQPDLILMDLRMPHRGGLSATQAISHVAPGARVVILTVEEGQARVGDAIQAGA
                     VGYLLKDIDGPELARSVRLAASGNAVIHPTLTRQFIEEIRQLTRGEQSVSALSCREIE
                     VLQMIAYGSTNREVAHALHISPQTVKTYLERIFTKLGVSDRTRAVAVALKHGIVE"
     gene            complement(7436..7684)
                     /locus_tag="VF995_03990"
     CDS             complement(7436..7684)
                     /locus_tag="VF995_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX9342761.1"
                     /translation="MDWRHRARCRDVDPELFFPVGTTGPAEAQVQAAKAICALCSVRE
                     ECLQWALDTAQDAGVWGGLSEEERRALRRGQRRRARIA"
     gene            8151..8999
                     /locus_tag="VF995_03995"
     CDS             8151..8999
                     /locus_tag="VF995_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX9342762.1"
                     /translation="MTNSELPRILGHRGARFTAPENTVASFQAAIAEGADGVELDLRR
                     TLDQALVCLHDPGLGRTTDGRGPVRQWTLEELRRLDASAGLKFGRNGSAGLPWTASPG
                     SPGSPARSAGDWPFRGIQIPTIAQALDALPRSALVDVEAKFRGEGPDGPAEVAELLAA
                     ELAGRPDRDRIMVSSFSRRLMAAAVPKLPGMRVGIVTSALTPLGRALRVAEAAGCTLL
                     AAQAHAYLGPMAKAAASRAADAGILLLAWTVDDPETALRLADLGVSVIVSDRPGALRQ
                     ALAQSP"
     gene            complement(8950..9900)
                     /locus_tag="VF995_04000"
     CDS             complement(8950..9900)
                     /locus_tag="VF995_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX9342763.1"
                     /translation="MRVALIVNPNATRVSQRLTDRVAKLVAAMHHKVEVQPTTQPRQA
                     TALASAAVAGGAELVVTLGGDGTVNEVANGMRGSGVPLGLAGGGKTNVFQRGLGLPSD
                     AVAATSRLLELLADGNGGRRRISLGAANGRAFTFAAGLGLDGAIVREVERRRRAKQLY
                     GDHAYVVAGLKTMLLTYDRDNPHLAIRFPDGRPALSGYFALVTNGDPYTYLRHRPFRP
                     TPEASFEGGLDVLVGQTMSAVRLTRALTGMLSHLPRPAYPGLPVLHDEEAFTLSSDVP
                     LAFQLDGEYLGDFTTVAFERLEGALTVIAPAPAAARRAGR"
     gene            complement(9942..10841)
                     /locus_tag="VF995_04005"
     CDS             complement(9942..10841)
                     /locus_tag="VF995_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="HEX9342764.1"
                     /translation="MAHPVSPPGAVNNENGASELTGGGGPSAAGHATAPPSDQDQNQN
                     QNRDQERERDRAADRAQARELFHRYAATRDPAVREQLVGMHLPLVEYLARRFRNRGEP
                     LEDLVQVGTIGLLKAIDRFDLERAVEFSTYATPTIVGELKRHFRDKGWAVRVPRRLQE
                     LNLSLTKAVAELSQEIGRSPTVREIAAKVHLSEDEVLEGLDTSNAYAMVSLDAPAGDL
                     DAPMVSDQIGAEDEGLVALEYRAALGPLIAELPERERRILYLRFFRGMTQSQIADRLG
                     ISQMHVSRLLNRTLSALRAGLLE"
     gene            complement(10801..11235)
                     /locus_tag="VF995_04010"
     CDS             complement(10801..11235)
                     /locus_tag="VF995_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9342765.1"
                     /translation="MASPSPSSVVHLEIPASGAYLAVARATATGLAAQLHFTYDQLDD
                     LRIAVDEACTQLLARAGGHGAGTLRVAYHLEEEAGELVVEVSVAAPDGASPVARDSFA
                     WQILNAMTDEVTEHLEESRLRLSLRKRGGHGAPGEPTRSGEQ"
     gene            complement(11311..12069)
                     /locus_tag="VF995_04015"
     CDS             complement(11311..12069)
                     /locus_tag="VF995_04015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9342766.1"
                     /translation="MSREDRRLGGRQGAYHRYAEVYDRVWKAAPHHRFVDLYLEAAKL
                     AGVAVHRVVDAACGTGTAALELARRGYRVTAFDLSGAMVSRARDKCLEAGLGDLHLLV
                     GDLRAVPLADGCADLVTVLNASINYLLDPEELVGAFGQLRRLVGERGAVVFEPLSARF
                     LYDGYEPNRHLTDGDFRLDASYELQDDLLVERIRWRVEGVEATECYRQRYYDDARLKS
                     LLAAAGLTVADHLPMYPAIPAEPARGRTLWIAVP"
     gene            12189..12602
                     /locus_tag="VF995_04020"
     CDS             12189..12602
                     /locus_tag="VF995_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX9342767.1"
                     /translation="MSTRRTTEQHGRSTRQRTAVLAALRRVPGPLSAQDLHARLDRTV
                     GLATVYRALQDLVDAGQVDVFRRENGEALFRLCNPVHHHHLVCQRCGRVEEIDACEVE
                     PWASRAAERRGFTVSGHQADVFGLCPRCQHATSAS"
     gene            complement(12631..>12898)
                     /locus_tag="VF995_04025"
     CDS             complement(12631..>12898)
                     /locus_tag="VF995_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342768.1"
                     /translation="KTITMYVSDIDQTEIPPGKVYQVQVRHPDGSTQQLDISEKNYRD
                     LKLEGMGKTLKKRGRKPGSTSKVAGATAAAVRTRRGPRAEDKLV"
BASE COUNT         1954 a         4466 c         4535 g         1943 t
ORIGIN      
        1 ctccgggcga ttacgagggg cttacggctg cccggccgcg acgccaagcg ggccgcttgc
       61 cgttattgtt tgagatatgc gggcagaacc ggaaccgcgg ctgcgggcct cggcggcgtt
      121 tgtcgccgga gtctcggcga tgctgctcgc gaccgggctg ctggggctcg ccagcctggt
      181 gcaggccagc gtgtcgtttc cgccgctcgg cctcgcgcag cgcttcctgc acctgattcc
      241 cgggcgggtg gacgtcttct tcatcgacct gatggggcac tgggccctgc ggtcgttcgc
      301 ggcgggcttc accgtcggca gcatcctcgc gggcggtgtg gccggcatcc tggtcgcccg
      361 cgtccccggc ccaggcagcg ggcgcaccgc cgccatctgg ggcgccgcgg cagtgctcgc
      421 cgcgctggcg ttcgctggct ggagctccgc gcccggaggg ccctcggcgc tcacctacgc
      481 cgccttggtc ggggccgccg gggcggtgtt cgcgctggcg ctcggcgggg cgctggggcg
      541 gctggagcgc gacgtggtcc caccccccgc ccgggggctc gggcggtccc ggcgggagtt
      601 cctgcgcgcc gcggtcggcg ctggcgggct ggtggtggcg ggcctggcgg tgcgccgctt
      661 cagcggcggc gccatcggcg gggaccgtgg cggcaagccg ctcgcccggc cggcgggcgc
      721 ggcgccggtc aaggcggcca cgccggcgcc agcggacgcc gccgacgacc gtgcgttcct
      781 gtcgatcccc gggctgaccc cggaggtcac cccgaaccgg ctccactacg tggtggacga
      841 gtcgatcatc gaccccaatg tcgacagcgg ttcctggcgg ctgcgggtcg acggactggt
      901 cggcgagccg ctcacgctga cctaccagga cctgctcgcc atgcccgcga tcgagcagta
      961 cgtcaccctg cagtgcatct ccaacctggt cgggggagac ctggtaagca ccgccaagtg
     1021 gaccggcgtg ccactcaagg cggtgctggc acgggctggc ggtgtggcgc cgggggcggt
     1081 tcgggtggtg ttccacgcgg tcggcggcta caccgactcg ctgccggtcg ccaaggcact
     1141 cgacggcgag acggtgggtg gcctatggca tgaacggcca gagcctgccg cgcgcccacg
     1201 gctacccggc gcggatcgtg gcgcctggta tctatgggat gaaatgcgtc aagtggctgc
     1261 aacgcatcga agtggtcaac tacgactatc aaggctattg gcagcgcggc gcgggctggg
     1321 acaacatcgc caccatcaag tccgccagcc gcatcgacgt cccccacgac ctcgccggcg
     1381 tcaagggcga gctgatggtc gccggcctgg cctgggcggg ggaccggggc atcgaccggg
     1441 tcgaggtgtc gttcgacggc ggcaccacct ggacgcccgc gatcctgcgc cgcgagctgg
     1501 ccagggccgc ctggcggcag tggcgcctgc cctggcggcc cagcggcgcc ggcaagacgg
     1561 tgctcaaggt ccgcgcggtc gacgggcagg gccagcccca gagcgccggc gtggccccgc
     1621 cctacccaag cggcgccacc ggctaccagc agatcgaggt catcaccagc acctagcagg
     1681 cacatagcca atcacaaggg gcagaccggg cagcggtacc gccgcctggg gcaccgtcgg
     1741 cgcgccgacg ggaatgcgac gggggcttcg gtggttgcag ccgacgtaac gtgaccggcc
     1801 acctcggtcc caacccggtc caacccggtc gcgttcgctg acctgacagc ggtcctgaca
     1861 cgtgagaggg catgttgcga actttgctgg agcgccagcg tccgctggag acgctcctgt
     1921 atcctgctgc ggagcaattg atccgatcgg gaggcagctg ggtgcaacct tcggctcgtg
     1981 aactcggcca agcagccgag agcgtggcag ccgatggttc ctctggcacc cgcgccccgg
     2041 ccgacgagac gcccgaggag cgcgagcggc gcttcgccga cgaggcgatc ccgctgctcg
     2101 acaccatcta cgccggcgcg ctgcggctga cccgcaatcc cgccgacgcc gaggacctgg
     2161 tccaggagac cttcctgcgg gcctacaacg ccttccacca gttcgagccc ggcacgaacc
     2221 tgaaggcgtg gctgtaccgg atcctcacca atacctacat caactcctac cgcaagcagc
     2281 aacgcgagcc gcaatcggtt cccaccgaag acgtcgacga cttctcgctg taccagcaca
     2341 tcgccaacgc caacccggca ggggcggagt cgcccgagga ggtcctcctc gaccagattc
     2401 ccgaggacga ggtccggcag gcgctcgact cgctgcccga gcagttccgc gtcgccgtgc
     2461 tcctcgccga cgtcgagggt ttctcgtaca aagagatcgc ggacatcatg gggacctcga
     2521 tcgggacggt gatgtcccgg ttgcaccggg gtcgccgcca gctccagaag cggctgtggg
     2581 acttcgccgc tggccgcggc ttgctcgagg agggacagga atgggtgcgc ccgaagtagg
     2641 cgactgcggc tgcgacgggg tcctcaacca cgtctggtcc tatctcgacc acgagagcga
     2701 tccgcccacc tgcgccgagc tggaggcgca cctgcggacc tgcacctact gccaggggct
     2761 ggtccagttc aacgagcagc tcaagaagct ggtgcgccgt tgcgccgacc ccggcccggc
     2821 ccccgccgcc gcgtcggcgc agctccgcgc ccgcgtccag gcgatcatca aggtgcgcag
     2881 gacgctctcc tgaggttctc actggtttcc gccacgcctg gaacctctta ggcccttttg
     2941 atgttccctg gtgtagagag aaggggacag ggcccatcgg ccgacgatgg ggaggacggt
     3001 gacggaagac gatgcgatca gagaacgtca agggacaccg gccgggcaac gacccagcac
     3061 cgaccaaggt ggagcgggtc gggcgagtgt gccgggaagc cgactgcgaa accaggctgt
     3121 cgatctacaa caaaaccggg ttctgctggc agcaccagcc gctgatcttt cccaactacc
     3181 gcggcaagcg cttggccaac tccggtagct agaacgaacc agcgatttca cgggggttga
     3241 ggcaagacgc cgcggccggc cccgtccagc gggccggccg cgcgacgtgc tgcctgatgg
     3301 acctggacgg cctcagctct tggtcttctg gaagatcccg gcgatctcgt cgatctgctc
     3361 gaggagcttc tgcgccaccg ccgggtcggt cgagtgcttg gcttcgccgg cctgcttggt
     3421 cgcctgccag aagagggcgt gcaggttggg gaactgctcc aggtgcggcg gcttgaagta
     3481 gtccgtccac agcacccaca ggtgatgctt cactagctca gcgcgctgct ccttgatgat
     3541 cagggcgcga gtccggaagt cctggtcatt ggactggtgg tacttctcga tgatcttcgc
     3601 caccgattcg gcctcgatac gggcctgagc cgggtcgtag acgccacagg ggaggtcaca
     3661 gtgcgcgtgc gcgaccctgg tcggctgcag ccatgtggcc aatcgcttgc ccatcataag
     3721 cagtgtccct ttcgtgtggg cctgttcttg tggacctgat cttatggatg catcctcggt
     3781 cgaacttacc accggtctcg ccgccgtcgc ccggcatcta acatcatcat tcatgaacat
     3841 ccgacgggcg gttccggcag ttgtcgtgct catcggcgcc gccggactgt tgacgctgac
     3901 gctgcgccgt acccggctgg aacccatgct ggtcgacggg gacagcatgc gcccgaccct
     3961 ggcgcccggc cagcgcatcg cggtcgcacc gctgaccggc ccgccgcctc gcggtgcgct
     4021 ggtggtgctg cgccgccccg cgtcgcaggg gcctcaaggg tcgcaggtgg agggcgagcg
     4081 gctcgaagtg gtcaagcggg tggccgcgct cccaggcgat cgggtctggc tggccggcac
     4141 cgagaccacg ctcgccgccg acgagtatct cgtgctcggc gacaaccccg cccgcagcat
     4201 cgacgggcgc agcttcggcc cggttcacag tgacgatttc gtcggtatcg tcagattcgc
     4261 ctactggcct ccacatcgcc tggcccggcg cggataggat tcattcgaca cagacggggc
     4321 cgatggctac agacggggcc gatggccacg ggccggagga gggagtcgag tgctcgtcgt
     4381 cgtggccggt ggggctggtt tcctcggcgc caacctggtc gaccggctcc aggccgaggg
     4441 cgatgaggtg gtggtcgtcg atgacctctc ccatggccac ctggccaacc tgaccgaggc
     4501 gcggcggcgg ggcggggtgc gcttccaccg gatggacatc gcccagggcg gcctggccac
     4561 cctggccgcg cggatccgcg ccgacgcctg ggtccacctg gccaccccga ccgacccggt
     4621 gcgcgcctgg accgacgcct ccaccgacgg gcgcctgctg gtgctgggcg cgaccgagct
     4681 gctgcaggtg gccgccgcca gcggcgcgcg ggtggtgctg gcgtcctccg gggcctacct
     4741 gttccccaat cagctgactg gggtgaccac ggccgacgac cagcccgcgc cgacccaccc
     4801 tcacggggcc gggcggctgg ccgtggaggc gtacgccaac gccttcgcgg cccgcgggct
     4861 ggagatcgcc gtgctcgcgc tggggaccct ctacgggccg cgccaggacc cgcagcggcc
     4921 cgggctggtc gccagggcgg cctggtcgat gctgcagggc aaggcgccca gggtcgaggg
     4981 ggacgggcgt cagtgccgcg acttcctgtt cgtggacgac gccgtggacg ccctggcccg
     5041 ggcggtccat accgacgtgc gcgggcggct gctgctcggg accgggtcgg ccacctcggt
     5101 gctggaggtg atcgagcagc tggcctcgct ggccggttgg gcgggggagc cggaatgggc
     5161 gccggcccgg ccgggggacc cgcggcgctg cgccctcgac cccaatcccg ctggtcacgc
     5221 gcttggctgg cagccgtgga cacgcttgga ggagggtctg gccatgacgg ttgagtggct
     5281 caaggcgcga cttccggccg atcaccgcta cactgagtga ccgtaactca ctggtggttc
     5341 cctatcactt actagggagg gtacagtagg tagcatccga gctgaaacgg cccaaccggg
     5401 ctatggtggt cgcttagtcc ggtctcaata atcgccttgt tcgtactgct ggctgcaggt
     5461 cgaccgacag ggccgtcccc gccgcacgtc cccaaattgc agtggtcgga cgaacatccg
     5521 gtccattcgg gccttggcct cggtgccagg acccgggctc gagaaaggga tgaacggtgg
     5581 tagtttctcg caaggcaacc cgtgtggagg ccccgagcta cctgcgcact gcggaggtag
     5641 ccgatatcct acacgtgtcg ccaaagacgg tctctcgctg ggccaaggaa ggcaagctcc
     5701 ccttcctcaa gacgctgggc ggccaccgcc gctacccgga ggcgaagatc caggagctcg
     5761 ccgaggagct gcgcgaagag ccaaccggct gagcggcccc aacccgtgat ccacgagatc
     5821 caaacgatcc acaagatcgc tccgcaccgc cgtcaccgct gacgcagacc gatcgctgac
     5881 gatcagtccg cccacgtcga ccagggcacc gtacgggcct catgggcgcc gtgggcggcg
     5941 tcagcgcggc ggcggcttgg cgtggcgagc accatcccct gcaacagcag gaaaatgcca
     6001 gccagctcgg ccacgtcgcc gacgctcacc acggcatgca ggggccgcac cgccagctga
     6061 tccccaaggg cggcaaggcg cgtgccgccg ccctccagga cgtgcttggc gtccgggcga
     6121 tgggcgagtg gccgttcaac ggtgatgccg gcatgctcca gggtcgtggc ggccaccggc
     6181 acgcccaggt tgaggatgag caccagcagg ttgcagagca gcccgagggc aagcagcggc
     6241 acgccgggaa gctggccgtt ggcccgggcg acgccgagca gcacgaccag ggtcagcgcc
     6301 agcagcagca cgccgacgcc gcgtggcagg ccgatcgagc gcaatgccag cgccccgatc
     6361 acgccctgca gcatcaggca gatcagtccc agcggtttgc cgcgcacact ggcctcggca
     6421 agcccctttg gctgaccgcc gcgaaccacg ccgatcccga tcccagcgac ggcgacgaac
     6481 agcaacagca gtagcaggcc catgcgcgag aatctaccac ttcgatccca ctgttcggcc
     6541 gccggccgag catgcaggcg agcgaagtgt tgcggtccat gggaggccgg cggagccgcc
     6601 gagccgctat tcgacgatgc cgtgcttgag ggcgacggcg accgcgcggg tccggtcgga
     6661 gacgccgagc ttggtgaaga tccgctccag gtaggttttg accgtctgcg gcgagatgtg
     6721 cagcgcgtgg gcgacctcgc ggttggtgga gccgtaggcg atcatctgca ggacctcgat
     6781 ctcgcggcac gacagcgccg agacgctctg ctcgccgcgg gtgagctggc ggatctcctc
     6841 gatgaactgg cgtgtgaggg tggggtggat gacggcgttg ccgctggcgg ccaagcgcac
     6901 cgagcgcgcc agctcggggc cgtcgatgtc cttcagcagg tagccgaccg ccccggcctg
     6961 gatcgcgtcg ccgacccgtg cctggccctc ctcgaccgtc aggatcacca ccctggcccc
     7021 cggggccacg tggctgatcg cctgggtggc gctcaggccg ccccggtgcg gcatgcggag
     7081 gtccatcagg atcagatccg gctgcagctc cgccgccaag cggaccacct cgtcaccgtc
     7141 gcgcgcctcg ccgaccacct tgagggtctc gtcgagggag agggcggcct tgatcccatc
     7201 ccgcgtcagg ggatggtcgt ctgccaccag gatgcgaacg acgccgtcgt ccatgctcca
     7261 ctcacagttc gacgcgccgc cccggctcgc tcaccgagcc gaagcggcgc tctcactggt
     7321 ttcctttgcc ccgcttgccg ttcgcgatcg taccgcttcc gcttgacgga aggaaaccgc
     7381 cgatccggac gcggcgaact ggatcagctc tcgacggcgg cgtggcagtg aaacgtcagg
     7441 cgatccgggc cctcctgcgc tggccgcgac gcagcgctcg gcgctcctcc tcggaaagac
     7501 cgccccaaac gccagcgtct tgggcggtgt ccagcgccca ttgcaagcac tcttcgcgga
     7561 ccgagcaaag cgcgcagata gccttcgccg cctgcacctg ggcctcggca ggcccggtcg
     7621 taccgactgg gaagaacagt tccgggtcaa cgtcacggca tcgggcacga tggcgccaat
     7681 ccattgacaa tcctcacttt ctcgtgcaaa gccctggggg aagggcaagt gctcgaatgc
     7741 ccgtgaaggg gtccaatgtt gcgacccttc catatgaaga gcgcgccgag tctaagacag
     7801 tgcgaagccc ttgggaagaa cccaattctc gcgagagtaa gggattccag cgcaaacctg
     7861 cgcgcatcag gcccctgacc ggccagttca ggccccagaa tcgcccatcc gcacccgtcg
     7921 aagagggcaa atatgaccct ctcggcgaag attgatgagt gggaaataac tagttacgcg
     7981 aatctcctcc atagaaatac gcaggtagca ttcatggtgg ggcaggaatg gatgaacttg
     8041 ccaatccgtc ctgctccaga agcgccatga agccgccagg cgcggtccgc caggcgcggt
     8101 ccgccaggcg cggtccgccg actcccccag tcgcgtagag tttgtccctg atgacgaaca
     8161 gcgagctgcc gaggatcctg gggcaccggg gcgcccgttt cacggcccct gagaacaccg
     8221 tcgcctcctt ccaggcggcc atcgccgagg gtgccgacgg cgtcgagctg gacctgcgcc
     8281 gcaccctcga ccaggcgctc gtctgcctcc acgacccagg ccttggccgt accaccgacg
     8341 gtcgcggacc ggtccgccag tggaccctgg aggagctgcg gcgcctggac gcctccgccg
     8401 gcctcaagtt cggccgcaac ggctccgccg gcctcccatg gacggcatcg cctggctcgc
     8461 ctggctcgcc tgcacgctcg gccggggact ggcccttccg cggcatccag atcccgacga
     8521 tcgcgcaggc gctcgacgcg ctgccccggt cggcgctggt ggacgtcgag gcgaagttcc
     8581 gcggcgaggg ccccgacggg cccgccgagg tggccgagct gctcgccgcc gagctggccg
     8641 ggcggcccga ccgcgaccgg atcatggtgt cttcgttctc ccggcgcctg atggccgcgg
     8701 ccgtgccgaa gctgcccggt atgcgggtcg ggatcgtgac ctcggcgctc accccgctgg
     8761 ggcgggcgtt gcgggtcgcc gaggcggccg gctgcaccct gctcgccgcc caggcgcacg
     8821 cctacctcgg cccgatggcc aaggcggcgg ccagccgtgc cgccgacgcc ggcatcctgc
     8881 tgctggcctg gacggtcgac gaccccgaga ccgcgctgcg cctggccgac ctgggggtca
     8941 gcgtgatcgt cagcgaccgg cccggcgcgc tgcggcaggc gctggcgcaa tcaccgtgag
     9001 ggcgccctcc agccgctcga acgccacggt ggtgaagtcg cccaggtact cgccgtccag
     9061 ctggaaggcg agcggcacgt cagaggagag cgtgaaggcc tcctcgtcgt gcagcaccgg
     9121 cagcccggga taggccggtc gtggaaggtg cgacagcatc ccggtgagcg cgcgggtgag
     9181 cctgaccgcc gacatcgtct ggccgaccag cacgtcgagg ccgccttcga agctggcctc
     9241 cggggtcggc cggaacgggc ggtggcgcag ataggtgtag gggtcgccgt tggtgaccag
     9301 cgcgaagtac ccgctgagcg ccgggcggcc gtcagggaag cggatcgcca ggtgcgggtt
     9361 gtcgcggtcg taggtgagca gcatggtctt caggcccgcc acgacataag catggtcgcc
     9421 gtagagctgc ttggcgcgcc ggcgccgctc gacctcgcgc acgatcgcgc cgtccagccc
     9481 gagccctgcc gcgaaggtga acgcccgccc gttggcagcg ccgagcgaga tccgtcgccg
     9541 tccaccgttg ccgtcagcca gcagctccag cagcctgctc gtcgcggcaa cggcgtcact
     9601 gggcagacca aggccgcgct ggaacacgtt ggtcttgcca ccaccggcca gcccaagcgg
     9661 cacgccgctg ccgcgcatgc cgttggccac ctcgttgacg gtgccgtccc cgccgagcgt
     9721 caccaccagc tcggcgccgc cggccaccgc cgcgctggcc agcgcggtcg cctgccgggg
     9781 ctgtgtggtc ggctggactt cgaccttgtg gtgcatggcc gcgaccagct tggcgacccg
     9841 gtcggtgagg cgctgcgaga ccctggtcgc gttcgggttg acgatcagcg cgacgcgcat
     9901 agcgcaagcc tacccgtaca agcacaccgc cagtccgagg gtcactcgag gaggccggcg
     9961 cgcagggcgc tgagggtgcg gttgagcagg cgcgagacat gcatctggga gatgcccagg
    10021 cggtcggcga tctgcgactg ggtcatgccg cgaaagaagc ggagatagag gatgcggcgt
    10081 tcccgttcgg gcagctcggc gatcagggga ccgagggcgg cgcggtactc cagcgccacc
    10141 aggccctcgt cctcggcgcc gatctggtcg gagaccatcg gggcgtcgag gtcgccggcg
    10201 ggggcgtcca gtgacaccat ggcgtaggcg ttggaggtgt caagaccttc gagcacctcg
    10261 tcctcgctca ggtgaacctt ggcggcgatc tcgcggacgg ttggggagcg gccgatctcc
    10321 tgggacagct cggcgaccgc cttggtgagg gagaggttca gctcctggag gcggcggggg
    10381 acccgcaccg cccagccctt gtcgcggaag tggcgtttca gctcaccgac gatcgtgggg
    10441 gtggcgtagg tggagaactc caccgctcgt tccaggtcga agcggtcgat ggccttcagc
    10501 aggccgatgg tgcccacctg gacgagatcc tccagcggct cgccgcggtt gcggaagcgg
    10561 cgcgccaggt actcgaccag cggcaggtgc atccccacca gctgctcgcg cacagcggga
    10621 tcgcgggtgg ccgcgtagcg gtggaacagc tcgcgcgcct gggcgcggtc agcggcgcgg
    10681 tcgcgttcgc gctcctggtc gcggttctgg ttctggttct ggtcctggtc gctcggcggt
    10741 gcggtcgcgt gaccagccgc ggacggccct ccgcccccgg tcaattcgga cgcaccattc
    10801 tcattgttca ccgctccggg tgggctcacc gggtgcgcca tggccgcctc gcttgcggag
    10861 gctgagccgg aggcggctct cctccaggtg ctcggtcacc tcgtcggtca tggcgttgag
    10921 gatctgccac gcgaaggagt ccctggccac tgggctggcg ccgtcgggcg ccgccaccga
    10981 gacctcgacg accagctcgc cggcctcctc ctccaggtgg taggcgaccc gcagggtgcc
    11041 ggcgccgtgg ccgccagcac gcgccagcaa ctgcgtacag gcctcgtcca ccgcgatgcg
    11101 gaggtcgtcg agctggtcgt aggtgaagtg gagctgggcg gccagcccgg tcgcggtggc
    11161 gcgcgccacc gccagatagg ccccggaggc ggggatctcg aggtgcacca cggaactggg
    11221 actgggactc gccattgact gccgctcctt cacaaccacc ggtaccactc tcggcggccg
    11281 ctccacaaat cctatgtgcc ggcgcaggac ctatggaacc gcgatccaaa gggtacgtcc
    11341 ccgagcgggt tctgcgggga tcgccggata cattggcagg tgatcggcca cggtcaagcc
    11401 ggcggccgcc agcaggctct tcagcctggc gtcgtcgtag tagcgctggc ggtagcactc
    11461 ggtcgcctcg acaccctcga cccgccagcg gatacgctcg accagcaggt cgtcctggag
    11521 ttcgtagctc gcgtccaggc ggaagtcccc gtcggtcaga tggcggttgg gttcatagcc
    11581 gtcgtagagg aagcgggccg agagcggctc gaacaccacc gcgccccgct cgccgaccag
    11641 ccgccgcagc tggccgaagg cgcccaccag ctcctcgggg tccagcaggt agttgatgct
    11701 ggcgttgagg accgtcacca ggtcggcgca gccatcggcc agcggcacgg cgcgcaggtc
    11761 gccgaccagc aggtggaggt cgccaagccc tgcctccagg catttgtcac gcgcccgcga
    11821 caccatcgcg ccagagaggt cgaaggcggt cacgcgatag ccgcggcggg ccagctcgag
    11881 cgcggcggtg ccggtgccgc aggccgcgtc gaccacccgg tggaccgcca cgccggcgag
    11941 cttcgccgct tccaggtaca ggtcaacgaa gcggtgatgc ggcgcggcct tccagacccg
    12001 gtcgtagacc tcggcgtagc ggtggtaggc accctggcgc ccgccgaggc ggcggtcctc
    12061 gcgcgacacg ccacctccct catatcgctc acgacgaccg ggctcggaat aatctagccc
    12121 attttaacta gtttcaacaa ggcaaacagg cagagacgct gccagcagcc acgagtaaac
    12181 tggaattgat gtctacgcgc cgcacaaccg agcagcacgg acggagcacc cgtcagagga
    12241 cggccgtgct ggcggcccta cggcgggttc cagggccgct ctcggcgcag gacctgcacg
    12301 cgcggctgga ccgcacggta ggacttgcga cggtctaccg cgccctgcag gacctggtcg
    12361 acgcgggtca ggtggacgtg ttccggcggg agaacggcga ggcgctgttc cggctctgca
    12421 atccggtgca ccaccatcac ctggtctgcc agcgttgcgg gcgcgtcgaa gagatcgatg
    12481 cctgcgaggt cgagccctgg gcgagcaggg cggcggagcg gcgcgggttc acggtcagcg
    12541 ggcaccaagc cgacgtgttc ggcctctgcc cccgctgcca gcacgcaacc tcggccagtt
    12601 gaggctccgg ggggtggaga gccaggccgg tcagaccagc ttgtcctcag cacggggacc
    12661 acggcgggtg cggacggcgg cggcggtggc accggcgacc ttggaggtgc tgccgggctt
    12721 gcggccacgc tttttcagcg tcttgcccat cccctccagc ttcaggtctc tatagttctt
    12781 ctccgagata tcgagctgct gcgtcgagcc gtcgggatgg cggacctgga cctgataaac
    12841 cttccccggg ggaatctcgg tttggtcgat gtccgagacg tacatggtga tcgtcttg
//
LOCUS       DASWDC010000126         5462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_698104, whole genome shotgun sequence.
ACCESSION   DASWDC010000126 DASWDC010000000
VERSION     DASWDC010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698104"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VF995_04030"
     CDS             complement(<1..474)
                     /locus_tag="VF995_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342769.1"
                     /translation="MRKHRAWLAADLPAALATLRDASRRHRSVVLAVGIPAVIAAVVF
                     AAVSVPFAHAASDPQDGSSGSGSSSSSTSTASCTGSSVAKSTDGGAAVTFNTSNNKLT
                     ACDIKKDGRSAVGEWGTSSTGTFTVVELDDGAGTADESDNAISVTKGQMIFIRACT"
     gene            complement(650..1801)
                     /locus_tag="VF995_04035"
     CDS             complement(650..1801)
                     /locus_tag="VF995_04035"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005569816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9342770.1"
                     /translation="MAEVFIVEAVRTPIGRKGGGLAAAHPADLGAHVLAALVRRSGID
                     PAVVDDVIFGCVDQVGAQAINLARTAWLAAGFPEEVPGTTVDRQCGSSQQAVHFAAQG
                     ILAGSYDLAIAGGVENMSMVPIGGNVIAGIQAGYGSPLAADGWLARYGDQEISQFRGA
                     ELIAERWDLSRLAMEELAFRSHTRAARAWAEGRFDREVVPYGAVTRDEGFRADVSLAK
                     MASLPTLSPTGRLTAATSSQISDGAAALLLASEAAVRRHGLRPRARIRAMTVVGADPV
                     LMLTGPIPATQRILKQTGLSLDDLDLVEINEAFAPVVLAWQRETGADLERVNVNGGAM
                     ALGHPLGATGAKLLTTMLHELERREGRYGLQTMCEGGGMANATIIERLA"
     gene            2327..4012
                     /locus_tag="VF995_04040"
     CDS             2327..4012
                     /locus_tag="VF995_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HEX9342771.1"
                     /translation="MHSILGVNCFSHDTAACLLLDGRIVAFAEEERFNRERHTKRFPD
                     AAIAFCLTRAGIGARDLDVVAFAHRPGLDFARGTLDALRRGSPKRLAVQAATDANLTR
                     KELAFRRRWSYGGRIAHVGHHDAHGASAFFASPFEEAAVLTLDRGGDFLSTTLQHGQG
                     STLRPLAAVRNPDSLGEVYSAFTWLLGFTPNADEGKVMGLAPYGSDKLDAELASLIHV
                     TPDGLFRVNLAQFAYQRQGGWFSKGFLDRYGPPRVPESELTELHANLAHAVQGLLEET
                     ALHVARELRRRSGSPNLCLAGGVALNSVMNSRLLKETDFERIFVQPAASDAGNALGAA
                     LWVWHQQLGKPRREAMTHAFHGQDHPAGAYEQALAGVGAGLVVRRVGDPAAEAARLLA
                     DGRVVGWFQGRAEVGPRALGARSILADPRRAAMKDIVNQRVKHREPFRPFAPSVLAER
                     GPDWFAGYCPSPFMLLILPVRADRRAAIPAVTHIDGSARPQTVDAATNPLFHRLISQF
                     EARTGVPVVLNTSFNLRGEPIVQRPAEAVADFLTTGMDALILGDYLVEKTSHH"
     gene            4021..4911
                     /locus_tag="VF995_04045"
     CDS             4021..4911
                     /locus_tag="VF995_04045"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX9342772.1"
                     /translation="MPNRHPNPLITTEELAELLDGPNPPVVLDARWSLQGPPARERYD
                     QAHLPGAVYVDLDTDLSDPQGPGTGRHPLPDPERFQATMRAAGVDADRPVVTYDSPDP
                     ISAAARAWWLLRYFGHPDVRVLDGGFAAWQAAGLSVTAVVPTPEPGDWQAQPGKLPLL
                     KARQVAGMARDHVLLDFRAPERYSGEHEPLDPVGGHIPGAGNLPASETLAADGHQLPP
                     GELRERFAASGIGEGTLVGAYCGSGVAASQGILALHAAGLDGALYVGSWSEWVSDPAR
                     PVATGPEPGEFPPRAPGEGD"
     gene            5029..>5462
                     /locus_tag="VF995_04050"
     CDS             5029..>5462
                     /locus_tag="VF995_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012769539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9342773.1"
                     /translation="MNIGYARVSTADQNLQLQKDALRKANCDTIREEVKSGKADVERP
                     VRDAILRELERGDTLTVWKLDRLGRSIRELDAIVRDLGQRGVKFKSVTETIDTSTPQG
                     KLFFWLLSAFAEFERAQLIERTKAGRAARIANGLHPGGPRSYG"
BASE COUNT          843 a         2038 c         1821 g          760 t
ORIGIN      
        1 ggtgcaggcc cggatgaaga tcatctggcc tttggtgacg ctgatggcat tgtcgctttc
       61 gtcggctgtg ccggcgccgt cgtcgagctc cacgaccgtg aacgtcccgg tcgacgaggt
      121 gccccactcg ccgacggcgg accggccgtc cttcttgatg tcacacgccg tgagcttgtt
      181 gttgctggtg ttgaaggtca ccgcggcgcc gccgtcggtg gacttcgcca ccgaactgcc
      241 cgtgcacgac gcggtcgagg tcgatgagga cgacgaaccg gagccggagg acccgtcctg
      301 ggggtccgag gcggcgtggg cgaacgggac ggacacggcg gcgaagacga ccgccgcgat
      361 cacggcaggg atgccgaccg ccagcacgac actgcggtgg cgccggctcg cgtcccgcag
      421 cgtggcgagc gcggccggga ggtctgccgc gagccaggcg cgatgcttgc gcatctttct
      481 cctctctgtc tgtcaaggcc ggagctgggg gagtgctcga ggcagggacc tgcgctcgaa
      541 agacctatgc gcacccggcc gcccagacgc gacacgtccg gcgggacgga aagaagcgag
      601 acgaaaggca ggaaaaggca ggaaagggca ggcaaagagc ggtggaagct caggcgaggc
      661 gttcgatgat ggtggcgttg gccatgccgc cgccttcgca catcgtctgc agcccgtagc
      721 ggccctcgcg gcgctccagc tcgtggagca tggtggtcag cagcttcgcg ccggtcgcgc
      781 cgagcgggtg gccgagcgcc atggcaccgc cgttgacgtt gacccgctcc agatccgcgc
      841 cggtctcccg ctgccatgcc agcaccaccg gcgcgaacgc ctcgttgatc tcgaccaggt
      901 ccaggtcgtc caaggacagg cccgtctgct tgaggatccg ctgggtcgcc gggatcgggc
      961 cggtgagcat cagcaccgga tcggcgccca ccaccgtcat cgcccggatg cgggcccggg
     1021 gccgcaggcc gtggcggcgc accgccgcct ccgaggcgag cagcagcgcg gcagcaccgt
     1081 cggagatctg gctggaggtc gcggcggtca ggcgaccggt cggactgagg gtcggcagcg
     1141 acgccatctt ggccagcgac acgtccgcgc ggaacccctc gtcacgggtg accgcgccgt
     1201 agggcaccac ctcgcggtcg aaccggccct ccgcccaggc acgcgccgcc ctcgtgtggc
     1261 tccggaacgc cagctcctcc atggcaaggc ggctcagatc ccagcgctcg gcgatcaact
     1321 cggcgccgcg gaactggctg atctcctggt cgccgtagcg ggccagccag ccgtcggcgg
     1381 ccagcgggct gccatagccc gcctggatcc cggcgatcac attgccgccg atcgggacca
     1441 tgctcatgtt ctcgaccccg ccagcgatcg ccaggtcgta gctgccagcg aggatcccct
     1501 gcgcggcgaa gtgcaccgcc tgctgcgagg agccgcactg ccggtcgacg gtggtccccg
     1561 ggacctcctc cgggaagcca gccgccagcc acgccgtccg ggccaggttg atcgcctgcg
     1621 cgccgacctg gtcgacacag ccgaagatca cgtcgtcgac caccgccggg tcgatcccgc
     1681 tgcgcctgac cagcgccgcc agcacatgtg cccccaggtc ggccgggtgc gccgcggcca
     1741 ggccgccgcc cttgcgcccg atcggggtgc ggaccgcctc aacgatgaag acctcggcca
     1801 tcgtccagcc tcctcgctcg aatcctgcta ccaggatgcc cgttcatgcc ggtgtttggg
     1861 agaggaagga tccggagaga tctctcacgc gggtgtcgtg cagcttctgt gaccgttcgt
     1921 gaccatgccg tgtcgtaggt ctctgccagt tcgttaccta cgcaagtaat gctcatgacc
     1981 atcaacagag ctaccgaaca caacgcgaca catcacggaa cgtgacgggg ggaggacacc
     2041 aatgcgactg cggaacctga cagcatggtg gcgcgggcgg aagcacgaac gcgacacgcg
     2101 cgccggcttc gacgccaccg gcgaattcga gctctacacg ctcctggacg aggcatcgaa
     2161 gccgtcgttc cgagcttgac gccacccacc cccccggacc caacaccaac accaacacca
     2221 actccaagac caccaccgcc cctggcgcaa ccgccggggg cggttgtatt tccgaaggtt
     2281 gtggtagaag tagaacttgg gtgggatcga ccggggagcg gcggcggtgc acagcatcct
     2341 tggcgtcaac tgcttcagcc acgacacggc cgcctgcctg ctgctggacg gccgcatcgt
     2401 cgccttcgcc gaagaggagc gcttcaaccg cgagcggcac accaagcgct ttcccgacgc
     2461 cgcgatcgcg ttctgcctca cccgcgccgg gatcggtgcg cgtgacctgg atgtggtcgc
     2521 cttcgcccac cggcccgggc tcgacttcgc ccggggcacc ctcgacgcgc tgcggcgcgg
     2581 ctcgcccaag cggctcgcgg tccaggctgc caccgacgcc aacctgaccc gcaaggagct
     2641 ggccttccgg cgccgctggt cctacggggg ccggatcgcc cacgtcggcc atcacgacgc
     2701 ccacggcgcc agcgccttct tcgccagccc cttcgaggag gccgccgtgc tcaccctcga
     2761 ccgcggcggc gacttcctgt cgaccaccct gcagcacggc cagggatcca cgctgcggcc
     2821 tcttgcggcg gtccgcaacc ccgactcgct cggcgaggtc tactccgcct tcacctggct
     2881 gctcggtttc acccccaacg ccgacgaggg caaggtgatg ggcctggcac cctacggcag
     2941 cgacaagctc gacgccgagc tggccagcct gatccacgtc accccggacg gcctgttccg
     3001 ggtcaacctg gcgcagttcg cctaccagcg ccagggcggc tggttctcca aggggttcct
     3061 ggaccgctac ggcccgcccc gcgtgccgga aagcgagctg accgagctgc acgccaacct
     3121 cgcccacgcc gtccagggcc tgctggagga gaccgccctg cacgtcgccc gcgagctgcg
     3181 gcggcgcagc ggctcgccga acctctgcct ggccggcggg gtggcgctca actcggtgat
     3241 gaacagccgc ctgctcaagg agacggactt cgagcggatc ttcgtccagc cggccgcctc
     3301 cgacgccggc aacgccctcg gcgcggcgct gtgggtctgg caccagcagc tcggcaagcc
     3361 ccgccgggag gcgatgaccc acgccttcca cggccaggac caccccgccg gcgcctacga
     3421 gcaggccctg gccggggtcg gcgccgggct ggtggtccgc cgcgtcggcg accccgccgc
     3481 cgaggcggcc cggctgctcg ccgacggcag ggtggtcggc tggttccagg gccgcgccga
     3541 ggtcgggccg agggcgctcg gcgcccgctc gattctggcc gacccgcgcc gcgcggcgat
     3601 gaaggacatc gtcaaccagc gggtcaagca ccgcgagccg ttccggccgt tcgcgcccag
     3661 cgtgctcgcc gaacgaggcc ccgactggtt cgccggctac tgcccgagcc ccttcatgct
     3721 gctgatcctg ccggtccgcg ccgaccggcg ggccgccatc ccagccgtca cccacatcga
     3781 cggcagcgcc cgcccgcaga ccgtcgacgc cgccaccaac ccgttgttcc accgcctgat
     3841 cagccagttc gaggcccgca ccggcgtgcc ggtggtgctc aacaccagct tcaacctgcg
     3901 cggcgagccg atcgtccagc ggcccgccga ggcggtcgcc gacttcctca ccaccgggat
     3961 ggacgcgctg atcctgggcg actatctcgt cgagaagacc tcgcaccact aaggagcagc
     4021 atgccgaacc gacaccccaa tccgctgatc accactgagg agctggccga gctgctggac
     4081 ggcccgaacc cgccggtggt gctcgacgcc cgctggtcgc tccagggccc gccagcgcgg
     4141 gagcgatacg accaggcgca cctccccggc gcggtctacg tcgacctcga caccgacctg
     4201 tccgacccgc aaggccccgg gaccggacgt cacccgctgc cggacccgga gcggttccag
     4261 gccaccatgc gggccgcggg cgtggacgcc gaccggccgg tggtcaccta cgactcgccg
     4321 gatccgatca gcgcggcggc ccgcgcctgg tggctgctgc gctacttcgg ccacccggac
     4381 gtccgcgtac tcgacggcgg tttcgccgcc tggcaggccg ccgggctgtc agtgaccgca
     4441 gtggttccca cccccgagcc aggcgactgg caggcacagc cgggcaagct gccgctgctc
     4501 aaggcgcgtc aagtcgccgg catggctcgc gaccacgtcc tgctggactt ccgggcgcca
     4561 gagcgataca gcggcgagca tgagccactc gacccggttg gcggccacat cccaggcgcc
     4621 ggcaacctcc ccgccagcga gaccctcgcc gccgacgggc atcagctgcc acccggcgag
     4681 ctccgcgagc gcttcgccgc ctccggcatc ggcgagggca ccctggtcgg cgcctactgc
     4741 ggctcaggcg tggccgcctc gcagggcatc ctcgctcttc acgccgcagg gttggacggc
     4801 gccctctatg tcggctcctg gagcgagtgg gtcagcgacc cggcccgtcc ggtcgccacc
     4861 ggccctgagc ccggcgagtt cccgccccgg gcgccgggcg agggcgacta gcccggcgcc
     4921 agttccgtcc agccgtcgcc acgccctctc cctggaacac cagctcagtg cagctgcacc
     4981 agcgggaagg cgtgtgatag gctttgctac atggagaggc gggacccggt gaacatcggc
     5041 tacgcgcgag tcagcaccgc tgaccagaac ttgcagctcc agaaggacgc actccgcaaa
     5101 gctaactgtg acaccatccg cgaagaggtc aagtccggga aagccgacgt cgagcgtccc
     5161 gttcgtgacg ccatcctccg ggaactggag cgcggcgaca cgctcacggt ctggaagctc
     5221 gaccggctcg ggcgcagcat ccgcgagctg gatgccatcg tgcgggatct tgggcaacgc
     5281 ggcgtcaaat tcaagtccgt caccgagacg attgacacct ctactcccca gggcaaattg
     5341 ttcttctggc tgctgagcgc cttcgcggaa ttcgagcgcg cccagctcat cgagcggact
     5401 aaagctgggc gggctgcccg gattgccaat ggcctccatc ctggcgggcc ccgaagctat
     5461 gg
//
LOCUS       DASWDC010000127         7978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_280668, whole genome shotgun sequence.
ACCESSION   DASWDC010000127 DASWDC010000000
VERSION     DASWDC010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280668"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2021)
                     /locus_tag="VF995_04055"
     CDS             complement(<1..2021)
                     /locus_tag="VF995_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9342774.1"
                     /translation="MVACTNSACSFGDPPEGAKFCPGCGRPLSLITAEGDESQRRTVT
                     IVYCDIQNSTTLSGRLDLEVLGNRLERFAQTAREVFTRHGGRAGTRQGDSMMGVFGIE
                     VVSDDHALRAVRAAAELRDALMPLADELATEFGLEFAVRIGVNTGQVLVRPGKEAVEE
                     QVEGQEVALAKRLEEHARPGQILVGEETYELVRDAIHTTEQVTLKLNGFREPKKAWRL
                     GEVYRGRPGRIPHLTVPLVGRQHELTLLKDLFQWLEAEKSRQLVTLLGEAGVGKTRLA
                     EEFVRGLGDQVTALRGHCLSYGEGVTFWPIKEIVRKAVGIKEAETTAAGSEQQLAKLS
                     KGLSELLSGDDRDREALTTVKQVLGIGGEEAPSPADTPVALARLLENLARRKPLVLLI
                     DDLHLADAALLDVLEKIAVAAPEREAPIMLLCMARPDELFVKREAWPKGRMNSFSLSL
                     PPLKQEEGEQLIGHLLPAMQLRNEELAHLTRLAQGNPLIVEELVAMFVGKGWLRLDEG
                     GWIATPDVYRYVAPPSVNVILQARLDKLDDEERKVIERAAVVGEQFHAIDVEALSPTL
                     DSTTVAGRLDALVRHQIIQEDQAVATTPTAVGSEGYRFRHILMRQAAYDLMTLPVRAE
                     LHERYADWLAVAAGERVSELDELIASHLYEAYKYLRRQGTPDERVTALG"
     gene            complement(2162..3562)
                     /locus_tag="VF995_04060"
     CDS             complement(2162..3562)
                     /locus_tag="VF995_04060"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX9342775.1"
                     /translation="MADIDAFARAEGEALIAELSEWCMIPSISTDPAYAVAVRRSAEH
                     LAGLMESAGLDADILSVDGSQPAVYGEWLGAGPDAPTITVYGHHDVQPVDPLELWDNP
                     PFEPVVRDGCLYARGASDDKGQIHFQVAAVRHLLAADGRLPVNLKFIVEGEEEAGSPN
                     IEAFLAEHADRLACDVIFVSDTGMYAEDVPTLVTAMRGLLYLQVDLRTATTDLHSGEF
                     GGTVPNAAAALVELLAKLKDRDGRIAVPGWYDDVLDLTPAERAHFAALPFDPDAFMRA
                     AGVRALPGERGWSPLERVGGRPSADINGVWGGYSGAGSKTIVPAEAHAKLSFRLVADQ
                     DPARLQPLFERWACDNAPAGAEIEVRPFGRVRPCVTPLDHPGNLAASRAIARAWGIDP
                     LFTREGGSGPERALSDALDAACVYIGVMLPEDHIHAPNERLLLANYFRGLRAAAYAYE
                     EFARPEVGGPLLARRR"
     gene            3855..4046
                     /locus_tag="VF995_04065"
     CDS             3855..4046
                     /locus_tag="VF995_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342776.1"
                     /translation="MELGSAAREDHQLHVGLPVGLADCSACLSAGSVRRGVCDICGTR
                     ADDGPPVEAWPQESDPIPA"
     gene            4376..5770
                     /locus_tag="VF995_04070"
     CDS             4376..5770
                     /locus_tag="VF995_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HEX9342777.1"
                     /translation="MTVAKSRNRLHRGDAGPGGQTRWLLLLGAAAVTLPGSLLGLVES
                     FHLAEPSVVPWQGALLFGLAVVGSAFMLAWAAEAVQVDVSASLAIAALALIAVLPEYA
                     VSFVFAWKSGVDVRQFGPGCAPPGGGAAPNCSLVLANMTGSNRLLIGIGWALVVLLAW
                     WRSRRGGERRDGITLPRVQSVELTYLALACAYSLTLPLKRTVTLLDAAVLVGIFVAYS
                     VRMARAPASEPELYGPAAAIGGLSRRRRRALIVALFLFAAGVILLVAEHFAEALVATG
                     SELGVNQFLLVQWLAPLASETPELLVAGFFAWRLKTSAALTTLVSSKVNQWTLLVGTL
                     PVVFAIASHGFHGLPIDPAQREELLLTAAQTVFALGILANLTLSVGEALGLFSLFAAQ
                     FVLRALLPEGARGTELVLLSAVYLLFGLALLVRHRHELPQRLRDGLVTPYAALEAPEP
                     EPGPRSGPGRDTRS"
     gene            complement(5767..6975)
                     /locus_tag="VF995_04075"
     CDS             complement(5767..6975)
                     /locus_tag="VF995_04075"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX9342778.1"
                     /translation="MLGFGAAVSTASSFRRALDETVPAALAQAGGDPDLAICFLSMEH
                     APASGDLVRVLAERTGGAMVLGCTAQGVIGDARELEDTPALALWVARLPGVRLRPFHL
                     RLVELDEGYAITGWPDLDPDLEGEERGERQYVVLLADPFSFPADQLVERLNEERPGLA
                     VIGGMASGATGPGGHRLLLGTDAFHRGAVGVVLSGPVEVRTVVSQGCRPVGAPYAVTR
                     ATGNLVQELGGRPAVQRLRELLSDANPEVQALLAGGVQIGRVIDEHKVDFECGDFLIR
                     NLLGADPESGALAVGDAVAVGQTMQFHVRDAATATEELETLLAPVPSWRPRPSGVLLF
                     SCNGRGRRFFGEPDHDASMVAQATAAVPMAGFFAQGELGPVGGRNFLHAYTASMALFC
                     EATAAGSGSR"
     gene            complement(7010..>7978)
                     /locus_tag="VF995_04080"
     CDS             complement(7010..>7978)
                     /locus_tag="VF995_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342779.1"
                     /translation="GGFSPAEIDQVMASDRVAGIAAVRGGLIGVRGTSASYRVVPVEV
                     MAVDPEAYASAAGRAGDALRKGLPHGVVLSQTGASLRHLTVGGRLRLTGGGAPLTVSG
                     VVDDQLLGGYEAAISRTLAPRFGLSHASYLLLRPRGTMADLEAAVQSLLHRRILRFVL
                     PGQRSWFRSGETLPLEQVKVRFGELSVQSLANPAPSPVWLAAKVVARNVPVLGQVHCH
                     RAIIADLSAAMTELQRLHLDRFIDLAAAGEHGGCMDPRHPVAVSASTGAALPLSHAAW
                     GIDLELGTTAKVDQRLVQVMAQHGFSWGGRWLPAAPNRFEWVGAGA"
BASE COUNT         1071 a         2813 c         2912 g         1182 t
ORIGIN      
        1 tggccaagcg ctgtcacccg ctcgtccggg gtgccctggc ggcgcaggta cttgtaggcc
       61 tcgtagagat gggaggcgat cagctcgtcg agctccgaga cccgctcgcc ggccgcgacc
      121 gccagccagt cggcgtagcg ctcgtgcagc tcggcgcgga ccggcaaggt catcaggtca
      181 tacgccgcct ggcgcatcag gatgtggcgg aaacgatagc cctcgctgcc cacggcggtc
      241 ggtgtcgtcg ccaccgcctg gtcctcctgg atgatctggt ggcgcaccag cgcgtccaga
      301 cgcccggcga cggtggtgct gtcgagggtc ggcgacagcg cctccacgtc gatggcgtgg
      361 aactgctcgc cgaccaccgc ggcccgctcg atgaccttgc gctcctcgtc gtcgagcttg
      421 tcgaggcgtg cctgcaggat cacgttgacc gacggcggcg ccacgtagcg gtacacgtcc
      481 ggggtggcga tccagccacc ctcgtccagg cgcagccagc ccttgccgac gaacatcgcc
      541 accagctctt cgacgatcaa cgggttccct tgggccagcc gcgtgaggtg ggccagctcc
      601 tcgttgcgga gctgcatggc ggggagcagg tggccgatga gctgctcgcc ctcttcctgc
      661 ttgaggggcg gcagcgacag cgagaagctg ttcatcctgc ctttgggcca agcctcgcgt
      721 ttgacgaaca gctcgtccgg ccgggccatg cagagcagca tgatcggcgc ctcacgctcc
      781 ggcgcggcga cagcgatctt ctcgagcacg tcgagcaggg cagcatccgc caggtggagg
      841 tcgtcgatca gcagcaccag cggtttgcgg cgtgccaggt tctccagcag ccgcgcgagt
      901 gccaccggcg tatcggccgg cgagggcgcc tcctcaccgc cgatgccgag aacctgcttg
      961 acggtggtga gcgcctcacg gtcgcggtcg tcgccgctga gcagctccga aagccccttg
     1021 gagagcttcg cgagctgctg ctcggaaccg gcggcggtgg tctcagcctc cttgatgccg
     1081 accgccttcc gcacgatctc cttgatcggc cagaaggtga cgccctcgcc gtaggagagg
     1141 cagtggcccc gcaacgcggt gacctggtcg ccgaggccac gcacgaactc ctcggccagc
     1201 cgggtcttgc caacgccggc ctcgccgagc agggtgacga gctgacggct cttttcggcc
     1261 tccagccact ggaacaggtc cttgagcagg gtcagctcgt gctgccgccc caccagcggc
     1321 acggtcaggt gcgggatgcg ccccggacgg ccccggtaga cctcgccgag gcgccaggcc
     1381 ttcttcggct cgcggaagcc gttgagcttg agggtgacct gctcagtggt gtgaatggcg
     1441 tcgcgcacca gctcataggt ctcctcgccg accaggatct ggcccggcct ggcgtgctcc
     1501 tccagccgct tggccagcgc gacctcttga ccctcaacct gctcctcgac cgcttccttg
     1561 ccggggcgca ccagcacctg gccagtgttc acaccgatcc gcaccgcgaa ctcgagaccg
     1621 aactcggtcg ccaactcgtc ggccaggggc atcagcgcgt cgcgcagctc cgccgccgcc
     1681 cgcaccgccc gcagggcgtg gtcgtcgctc accacctcga tgccgaacac gcccatcatg
     1741 ctgtcgccct gccgggtccc ggcacgaccg ccatggcggg tgaagacctc gcgggcggtc
     1801 tgggcgaagc gttccagtcg gttgcccagc acctccaggt cgagccggcc gctgagggtg
     1861 gtggagttct ggatgtcgca gtagacaatc gtgaccgtac gccgctggct ctcgtcaccc
     1921 tcggccgtga tcaggctgag cgggcgccca catcccgggc agaacttcgc cccctctgga
     1981 gggtcgccga agctgcacgc gctgttcgtg caagccacca tactcgtcgc cacgaccgcc
     2041 gccccgatcc ccggttctgt cgctggtttc aaatagcaca ctggtgtcgc caaggctgtc
     2101 gcattgctga caggccagtg aagtgagcac gatcgaggcc gctcttcatg gtcgcccggc
     2161 atcacctccg gcgggcgagc agggggccgc cgacctcggg gcgggcgaac tcctcgtagg
     2221 cgtaggcggc ggcgcgcagg ccgcggaagt agttggcgag cagcaggcgc tcgttggggg
     2281 cgtggatatg gtcctcgggg agcatcacgc cgatgtagac acaggcggcg tcgagggcgt
     2341 cgctgagcgc tcgttcgggg cccgagccgc cctcgcgggt gaacagtggg tcgatgcccc
     2401 aggcgcgggc gatggcgcgg ctggcggcga ggttgccggg gtggtcgagg ggggtgacgc
     2461 agggccggac gcggccgaag gggcggacct cgatctcggc gccggccggg gcgttgtcgc
     2521 aggcccagcg ttcgaacagg ggctggagcc tggcggggtc ctggtcggcg accaggcgga
     2581 aggacagctt ggcgtgggcc tcggcgggga cgatcgtctt cgacccggcg ccgctgtagc
     2641 cgccccagac gccattgatg tcggcgctcg ggcggccgcc gacgcgctcc agcggcgacc
     2701 agccccgctc gccggggagg gcgcgcacgc cggcggcgcg catgaaggcg tcgggatcga
     2761 acggcagggc ggcgaaatgg gcccgctccg ccggggtcag gtcgagcacg tcgtcgtacc
     2821 aaccggggac ggcgatgcgg ccgtcgcggt ccttgagctt ggcgagcagc tcgaccaggg
     2881 ccgcggccgc gttcggcacg gtgccgccga actcgccgga gtgcaggtcg gtggttgcgg
     2941 tgcgcaggtc cacctgcagg tacagcaggc cccgcatggc ggtcaccagg gtggggacgt
     3001 cctcggcgta catgccggtg tcggagacga agatcacgtc gcaggcgagc cggtcggcat
     3061 gctcggccag gaacgcctcg atgttggggg agccggcctc ctcctcgccc tcgacgatga
     3121 acttgaggtt gaccggcagg cggccgtcgg cggcgagcag gtgacgcacg gccgccacct
     3181 ggaagtggat ctggcccttg tcgtcggagg caccgcgggc gtagaggcaa ccatcccgca
     3241 ccaccggctc gaacggcggg ttgtcccaca gctccagcgg gtcgaccggc tgcacgtcgt
     3301 ggtggccgta cacggtgatc gtcggggcgt ccggcccggc gccgagccac tcgccataga
     3361 cagcgggctg gctgccgtcg accgagagga tgtccgcgtc gaggccggcg gactccatca
     3421 gcccggccag gtgctccgcc gagcgccgca ccgccaccgc ataggccggg tcggtggaga
     3481 tcgacgggat catgcaccac tcggacagct ccgcgatcag cgcctcgcct tcggccctgg
     3541 cgaacgcatc gatatcagcc acaggtgctc ctggtcctgg acggcactgc tttcgctcgc
     3601 ttgcatgctc ggcggctccg ccggcctccc atgggcggca tcgcattcgc tcgcttgcat
     3661 gctcggccgg cggggtaagg agtctggttg gaccgacgca gggtagcaga ccccatcaga
     3721 ctacaaacaa acactctcct tacccccgcg cccgtactgt ccttacctga gcaggctacc
     3781 gttgcagcat cgtgactagg ccaattgcgg tagttcctct acagtgaatc tcccaccgga
     3841 gaaagaaggc cccgatggaa ctgggcagcg ccgctcgtga agatcaccag ctccatgtcg
     3901 ggcttccggt cgggcttgcc gactgctccg cctgcctgag tgccggctcg gttcggcggg
     3961 gtgtctgcga catctgcggc acccgcgccg acgatggccc gcccgtggag gcgtggccgc
     4021 aggaaagcga cccgatcccc gcctgactcc cgaccactga ccgcgcgggc ctcggcggag
     4081 gcccgccgcc ttgtcccggt ccgccttcgg ctccgttccc cgccttgttc gtctgccctc
     4141 tgggcagcgg cagtgccggc ctgtaggatg gtcggcgaag gggagtagtc tccgggttgg
     4201 ccgtctggcc gcccggcgag ttcgccgtca ggacggtggc gtgccgttgc gccgcccggc
     4261 gcctcacccg tctgtcagat ggtccggccg gtccggctgg atcgccgcgt tgacgggcag
     4321 acgagacctt cggttagcag catcccttgt ggtctgcaga gccgaaggag cgcccgtgac
     4381 cgtcgccaag tcccgcaatc gcctccaccg gggcgatgct gggccaggcg gccagacccg
     4441 ttggctcctg ctgctgggtg cggcggcggt gacgcttccc ggcagcctcc tcgggctggt
     4501 ggagagcttc cacctggccg agccgtcggt ggttccgtgg cagggggcgc tgctgttcgg
     4561 gctggcggtg gtgggttccg cgttcatgct ggcctgggcc gccgaggcgg tgcaggtgga
     4621 cgtgtcggcg agcctggcga tcgcggcgct ggcgctgatc gcggtcctgc cggagtacgc
     4681 ggtcagcttc gtgttcgcct ggaagagcgg cgtcgacgtg cgccagttcg gtccgggctg
     4741 cgccccgccc ggcggcggcg cggcgccgaa ctgctcgctg gtgctggcga acatgaccgg
     4801 ttccaaccgg ctgctgatcg ggatcggctg ggcgctggtg gtgctgctcg cctggtggcg
     4861 gagccgccgc ggcggcgagc gccgcgacgg gatcacgctg ccgcgcgtcc agtcggtgga
     4921 gctgacctat ctggcgctgg cgtgcgccta ctcgctgacg ctgccgctga agcggaccgt
     4981 cacgctgctg gacgcggccg tgctggtcgg tatcttcgtc gcctactcgg tgcggatggc
     5041 gagggccccg gccagcgagc cggagctgta cgggccggcc gccgcgatcg gcgggctgtc
     5101 ccggagacgg cggcgggcgc tgatcgtggc gctgttcctc ttcgccgccg gggtgatcct
     5161 gctggtggcc gagcatttcg ccgaggcgct ggtggcgacc ggcagcgagc tcggcgtcaa
     5221 ccagttcctg ctggtgcagt ggctggcgcc gctcgcctcg gagacgccgg agctgctggt
     5281 cgccgggttc ttcgcctggc ggctgaagac cagcgcggcg ctgaccacgc tggtgtcgtc
     5341 caaggtcaac cagtggacgc tgctggtcgg cacccttccg gtggtgttcg cgatcgcctc
     5401 ccacgggttc cacggcttgc cgatcgaccc ggcgcagcgt gaggagctgc tgctgaccgc
     5461 cgcccagacc gtgttcgcgc tggggatcct cgccaacctg accctctcgg tgggcgaggc
     5521 gctcggcttg ttctccctgt tcgcggccca gttcgtgctg cgcgccctgc tgccggaggg
     5581 tgcccggggc accgaactgg tactgctctc cgccgtctac ctgctgttcg gtctcgccct
     5641 gctggtgcgc caccgccacg agctgccgca gcggctgcgc gacggcctgg tgacccccta
     5701 cgccgcgctg gaggcgccgg aacccgaacc agggccaagg tcagggccag ggcgcgacac
     5761 ccggagctag cgcgaacccg agccggcggc ggtcgcctcg cagaacaacg ccatgctggc
     5821 ggtgtaggcg tgcaggaagt tgcggccgcc gacggggccc agctcgccct gggcgaagaa
     5881 gccggccatc gggacggcgg cggtggcctg ggcgaccatg gaggcgtcgt ggtcgggctc
     5941 gccgaagaag cggcgcccgc ggccgttgca ggagaacagc agcaccccgc tcggccgcgg
     6001 gcgccaggac gggaccgggg cgagcagcgt ctccagctcc tcggtggcgg tggcagcgtc
     6061 gcggacgtgg aactgcatgg tctggcccac cgccacggcg tcgccgacgg cgagggcgcc
     6121 gctctccggg tcggcgccga ggaggttgcg gatcaggaaa tccccgcatt cgaagtcgac
     6181 cttgtgctcg tcgatcaccc ggccgatctg gacgccgccc gcgagcagcg cctgcacctc
     6241 ggggttggcg tcgctgagca gctcgcgcag tcgctgcacc gccggtcggc cacccagctc
     6301 ctgcaccagg ttgccggtgg cgcgggtgac ggcatagggc gcgccgaccg ggcggcagcc
     6361 ctggctcacc accgtgcgga cctcgaccgg gccgctcagg acgacgccga cggcgccccg
     6421 atggaaggcg tcggtgccga gcagcaggcg gtggccgccg ggcccggtcg cgcccgacgc
     6481 catcccgccg atcaccgcca ggccgggccg ctcctcgttg agccgctcga ccagctggtc
     6541 cgccgggaag ctgaacgggt cggcgagcag caccacgtac tgacgctccc cgcgctcctc
     6601 gccctccagg tccgggtcca ggtcgggcca gccggtgatg gcgtagccct cgtccagctc
     6661 caccagccgc aggtgaaacg ggcgcaggcg cacgccgggc aggcgcgcga cccacagtgc
     6721 caacgcgggg gtgtcctcca gctcccgcgc gtcgccgatc accccctggg cggtgcagcc
     6781 gagaaccatc gcgccgccgg tgcgctccgc cagcacccgc accaggtcgc ccgaggccgg
     6841 cgcgtgctcc atggagagaa agcagattgc caggtcagga tcgccgccgg cctgcgcgag
     6901 ggcggccggg acggtctcgt ccagcgccct gcggaagctg gaggcggtgg agacagcggc
     6961 gccgaaaccg agcatgcgag tcaccttact tcacttcgtg cgccggacgt caggcgccgg
     7021 cgcccaccca ctcgaagcgg ttgggagcgg cgggcagcca gcgcccgccc cagctgaagc
     7081 cgtgctgggc catgacctgg accagccgct ggtcgacctt ggcggtggtg cccagctcca
     7141 agtcgatccc ccacgccgcg tggctgagcg ggagcgcggc accggtggag gcggagacgg
     7201 ccaccgggtg gcggggatcc atgcagccgc cgtgctcgcc ggcggccgcc aggtcaatga
     7261 agcgatccag gtggaggcgc tgcagctcgg tcatggcggc gctcaggtcg gcgatgatcg
     7321 cgcggtggca gtgcacctgc ccgagtaccg ggacgttgcg ggcgaccacc ttggcggcca
     7381 gccagacggg gctcggggcc gggttggcga ggctctggac cgacagctcc ccgaagcgga
     7441 ccttgacctg ctccagcggc agggtctcgc cggagcggaa ccagctccgc tggccgggga
     7501 ggacgaaccg gaggatgcgg cggtgcagca ggctctggac cgccgcctcc aggtcggcca
     7561 tggtcccgcg ggggcgcagc agcaggtagc tggcgtgcga cagaccgaac cggggcgcca
     7621 gggtgcggct gatagccgcc tcgtagccgc ccaggagctg gtcgtcgacc acgccgctga
     7681 cggtcagggg ggcgccgccg ccggtcagcc gcagcctgcc gccgacggtg aggtggcgca
     7741 ggctggcgcc ggtctgggac agcaccaccc cgtgcggcaa gcccttgcgc agcgcgtcgc
     7801 cggcccggcc ggcggcgctg gcgtacgcct ccgggtcgac cgccatcacc tcgaccggga
     7861 ccacccggta gctggcgctc gtgccccgca cgccgatcag gccgccgcgc accgccgcga
     7921 tcccagcgac ccggtcggag gccatcacct ggtcgatctc agccggggag aagccgcc
//
LOCUS       DASWDC010000128        12077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_182953, whole genome shotgun sequence.
ACCESSION   DASWDC010000128 DASWDC010000000
VERSION     DASWDC010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12077
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182953"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(471..2045)
                     /locus_tag="VF995_04085"
     CDS             complement(471..2045)
                     /locus_tag="VF995_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX9342780.1"
                     /translation="MDEWPRGQGGGGAGRGTGSLPPEVRRAIRRAAPSRERQQHQRRQ
                     RRLIIALVSVLVVALVVAVGGYGYAHWRFGQIASINLPGLHKAAQPGKPMVLLVVGSD
                     SRSALTQAGDKAEFGSSQDASGQRSDVIMLVRIVPAAHQIKILSIPRDTLVPIAGTSG
                     RNKINAAFDKGPEQLIETIQQDFGIPINHYLLVNFDGFRSIINALGGINLDFPFPVRD
                     WDGSHNLSGLNITRTGCLHLSGDQALSVARSRHYQYLKNGRWHDDPLSDLGRIQRQQT
                     FLRVVLKKGLSEGLTNPLKANQFIGAVVHQLTIDSGMSVGTAVSLARQFRSFDPNQLA
                     NVTMPTTVANHYQSFGDVLLPNPAEDQHVIASFLGQTTPSPSPSSSGSGSGAASLAKI
                     GVKVQNGTKIGRLAARVAGQLHGIGVSGAVVDGNALPATIDPAGATSIRYPAGAQGLA
                     AAQSLGGMILGTVQLRQDATLPASTLVVVVGTGYKGLRNAPAAASTATTAAGPPTTAK
                     PATPSSSIRDFDPHPC"
     gene            complement(2144..3304)
                     /locus_tag="VF995_04090"
     CDS             complement(2144..3304)
                     /locus_tag="VF995_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX9342781.1"
                     /translation="MAEVEIGIGKSGRRAYGFDDIAIVPSRRTRDPDDIDISWDLDAY
                     QLALPLLASAMDAVVSPRTAIELGRLGGLGVLNMEGLWTRYEDPDILYQEIAGLPDAE
                     ATKRMQAIYREPIKPELIGQRVKEIKDAGALAAGSLTPQRVARYHQHALDAGLDVLVI
                     QGTVVSAEHVSQTTEPLNLKTFIARYDLPVIVGGCASYSTALHLMRTGAAGVLVGVGP
                     GAACTSRGVLGIGVPQATALADAAGARVRHLEETGRYVQVIADGGMRTGGDVAKAIAC
                     GADAVMIGSPLARAVEAPGRGYHWGMATFHPDLPRGTRVKTSALGTLKEILLGPAPVN
                     DGTMNLFGALRTSMATCGYQTLKEFQKAEVMVAPALQTEGKSLQRSQGVGMG"
     gene            3532..4023
                     /locus_tag="VF995_04095"
     CDS             3532..4023
                     /locus_tag="VF995_04095"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HEX9342782.1"
                     /translation="MPDPLDPFGSEDESESSDFEPIDEEERTALLEDLRDLAEFREAL
                     EPRGFRGVVIPCPDCEEDHYFSWSLLRENLEHILDHGEPRVHEPAFEPQVDHYVTWDY
                     GRGFVDGLLEGERETASTALRDEWTSPVEASRRLHRALLTRGLTEDEVAAVLNEGGLP
                     PAE"
     gene            complement(4052..4501)
                     /locus_tag="VF995_04100"
     CDS             complement(4052..4501)
                     /locus_tag="VF995_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9342783.1"
                     /translation="MGVKAKVPDKLRRQMVLPVPRKLVWQALTDPDQLAGWFCNSAEL
                     DLRPGGAVRFDFGAGERASAEIERVEARRRLAFRWRPAGDDPATTLVQFALEDVPRGT
                     RLTLTESGFAGLAEEVRERAYLDNEWGWDDELGELRGYLRVPVPMRM"
     gene            complement(4492..5331)
                     /locus_tag="VF995_04105"
     CDS             complement(4492..5331)
                     /locus_tag="VF995_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX9342784.1"
                     /translation="MTTPRPAEQAKAAFFDVDKTLLPGSSLYLFARGLYRRGFYSWRD
                     VAGFAYGQFTFRLTGAEGRGGMEAARESALAFVEGKRRDELTQLGHDIVVEVIGPRIY
                     PGMRRVVDAHHAAGDVTWLVTAAPRELAGEIARYLGMDGALGTEAELADGVYTGRLLG
                     PVLHGPAKVEAVTALAAERGIDLAASSAYSDSVNDRPLLEGVGHPVAVNPDRMLRQLA
                     AERGWPVQDFRRHRRLWLVWLPSGVAVAASGVAVAAAVASWRDRLRNRRHDRGLGRER
                     QWG"
     gene            complement(5328..6278)
                     /locus_tag="VF995_04110"
     CDS             complement(5328..6278)
                     /locus_tag="VF995_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX9342785.1"
                     /translation="MPRAVITGGAGFLGSNLCRRLLAEGFDVVCMDSLLTGRAANVDE
                     LQGPGFRLIDVDVTNFIHVAGPVDVVLHFASPASPIDYLRYPIQTLKVGALGTYRALG
                     LAKDKRARFLLASTSEVYGDPQVHPQPEIYWGHVNPIGPRGVYDEAKRFAEALTMAYH
                     RSHGLDTRIVRIFNTFGPFMRIDDGRAFCTFAVQALRGEPLTVHGDGSQTRSLCYVDD
                     LVEGIWRLLWSDLSGPVNIGNPEEVTVLELAKLVARAAGQDDPRIVFKPRPEDDPEVR
                     RPDITLATSRLGWKPQVSLAEGIERTLPWFRTQLGLDQPK"
     gene            complement(6282..7640)
                     /locus_tag="VF995_04115"
     CDS             complement(6282..7640)
                     /locus_tag="VF995_04115"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX9342786.1"
                     /translation="MRVTVIGAGHVGLVTAVCLAKIGHEVVADDDDQAKIDLMRSGKA
                     WFYEPDLEPLMAEALANGRLRFTSDKGEAVRHGEVIFVSVGTPPRPDGSPNLRFVEAV
                     AREVARNLPVGEFRLICEKSTVPVQTGERVAQVIAREAPEGVDYEVASNPEFLREGSA
                     VEDTLRPDRIVVGTESQRGTEVLRQLYAPITERPGTEFLATDRNTAELIKHASNAFLA
                     TKISFINQVARVCERAGADVELVARGMGLDPRIGAAFLRAGPGYGGSCFPKDVAAFAH
                     RSQELGVDFAILREVARVNSDARRAIVEKVRHLLWHLEGKHIGVLGLSFKPDTDDLRE
                     SPAIDVARMLLDEGASVLAYDPVASSAEVTQLAPGVELAAKAADVAIGAHALVLMTEW
                     GEFAELDPADLAGRMAYPILVDARNALDQAAFVAAGFTVAGVGRPPAGPASDAHHAHR
                     AG"
     gene            7807..8994
                     /locus_tag="VF995_04120"
     CDS             7807..8994
                     /locus_tag="VF995_04120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX9342787.1"
                     /translation="MRHRVRLAAVATALAAALAAGLAPGPAFAATATAAAAGPSVAGR
                     SAILVDAASGAILWSRDPATPRAPASLTKMLTALLVRATLPLDAVAVAGKDAATAAPT
                     RLGLRAGQKLRVGPALEAMMILSANDVAVLLADRAGGSPQRFQAAMNAEAALLGLRSS
                     AWHSPNGLDNPGHQASAYDLAILGRAVLRDPWLARVVRMRQTTITGPDGHPRGLHTHS
                     GFLVSYPGAIGIKTGFTNGAGHCLAAAATRHGRTLIAVVLDSPSPPADAAQLMNWGFA
                     GGSSGSSGGSSRRVSAATAAVAILPPYVQPVGVNSLVPPATVPAASLVTPAVPRGPGG
                     GCPGTAARPLRRRGRGSVRPAAGRRCRVAHQPAAPAAALPLQRRAWRHHDQLQPLSRF
                     RRW"
     gene            complement(8981..9652)
                     /gene="thiE"
                     /locus_tag="VF995_04125"
     CDS             complement(8981..9652)
                     /gene="thiE"
                     /locus_tag="VF995_04125"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX9342788.1"
                     /translation="MAADPDDRAADRRQARRERLAAARLYVCTPIRPDLAAFADAIYG
                     AGVDLVQLRDKRAEAVPLLRAAAVLREAADRHGGLLAVNDRADVALAAGADILHLGQD
                     DLPMAWAERIVGGDGLLLGRSTHDLGQARRAIEEGWDYFAVGPVHATATKPGRPAVGT
                     GLLEAVAALHPPVPWFAIGGIDRATLPAVSAAGATRAVVVRAITDAPDPAAAAAELRA
                     QLTTA"
     gene            complement(9642..10442)
                     /locus_tag="VF995_04130"
     CDS             complement(9642..10442)
                     /locus_tag="VF995_04130"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018837684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX9342789.1"
                     /translation="MDDPLTIAGQAFSSRLIMGTGGASSMEALERALVASGAELATVA
                     LRRTSPDARGSVLDVLRRVGCKLLPNTAGCFTARDAVRTARLAREAFETDWIKLEVIG
                     DDRILLPDGAGLLDAAETLVDDGFTVLAYSNDDPILARQLQGLGCAAVMPLGSPIGSG
                     MGIRNPYNLRIILEHATVPIVLDAGIGTASDAAVAMELGCDAVLLASAVTRAREPALM
                     AEAMRKAVEAGRLAHLAGRIPRRLYAEASSSFEGLADLSAPLPEPDGR"
     gene            complement(10447..10653)
                     /gene="thiS"
                     /locus_tag="VF995_04135"
     CDS             complement(10447..10653)
                     /gene="thiS"
                     /locus_tag="VF995_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017541361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX9342790.1"
                     /translation="MTVLVNGVPTELDPGATVAAALGVAGHTPTATGIAVALNGEVVP
                     KGAWTTTTLGEQDRVEVLGAAQGG"
     gene            complement(10650..11855)
                     /gene="thiO"
                     /locus_tag="VF995_04140"
     CDS             complement(10650..11855)
                     /gene="thiO"
                     /locus_tag="VF995_04140"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006143607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEX9342791.1"
                     /translation="MREPAGTGHAPDLVIVGGGVIGLGIAWRAAGRGLAVTVVDDQPG
                     HGASWAAAGMLAPVGEVHYGEQALLRLNLAAAERWPGFAAELEELSGCQVGYRRNGTL
                     IVARDGDDNAALDDLYRFQRGLGLESERLRGRECRQLEPGLAPGVRGGILASSDAQVD
                     NRAVAEALRAACERAGVRVAPGRVATVHLEGVGPRRARGVTLATGERIDAGTVLLAAG
                     CWSAGLEGVPADLVPPVRPVKGQLLHLRGPQEAPVCRHNVRGLDVYVVPRGDGRVVVG
                     ATVEERGFDATVTVRAVHDLLRAALELLPDTGELRLIETVAGLRPGSPDNAPLLGQLS
                     DGLVVATGHYRNGILLTPVTADAIAELLATGQVPELIAPFSPLRFGAAAATAAPATAA
                     AAETGGGQP"
     regulatory      complement(11850..11966)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         1615 a         4290 c         4482 g         1690 t
ORIGIN      
        1 gtgatgcgga agttgccgta gacctccttg ttgtactgct cggtgagcgg tagtccgacc
       61 gatgcatggg cgggcgtgtg cgacagcacc gccggtgcaa cggccgccac caggacggcg
      121 gcggtgagca cggcgcgcga cacgagcccc aaccgtcggg atcgtgctcg cgccactctc
      181 gatcgcgctt ctcccatact tcccccttgc gacgggcttg gctgatttgc gtgagcgccg
      241 gcggtcgcca ctgcccgtcg catccatgcg ggcggcctcc ggcggcggcg taataaaagc
      301 cgttctcctc gatcaagtca agctctctaa agtcttcaaa acatcaatgc tggatgggtg
      361 aggcgggctc gcaggaacag gcaggcaaca cccacactcg gggcataggt tgctctgcct
      421 gggaagatgc gccggcgcgg cggtcctgcc gacctcgcgg taccgcggtc tcagcagggg
      481 tgcgggtcga agtcccggat cgagctggag ggcgtggccg gcttggcggt ggtggggggc
      541 ccggccgccg tggtggccgt ggaggctgcc gccggggcgt tgcggagccc cttgtagcca
      601 gtgcccacca cgaccaccag cgtcgaggcg ggcagggtgg cgtcctgccg cagctggacg
      661 gtcccgagga tcatcccgcc gagcgactgg gcggcggcca ggccctgggc gcccgccggg
      721 tagcggatgc tggtggcacc ggccgggtcg atggtggcgg ggagggcgtt gccgtcgacg
      781 acggcgccgc tgacgccgat cccgtggagc tggccggcga cccgggcggc cagacggccg
      841 atcttggtcc cgttctggac cttgaccccg atcttggcga gcgaagccgc gccggagccc
      901 gacccggacg aggacgggga cggggacggg gtggtctggc cgaggaagct ggcgatcacg
      961 tgctggtcct cggcggggtt gggcagcagc acgtcgccga agctctgata gtggttggcc
     1021 accgtggtgg gcatcgtcac gttggcgagc tggttggggt cgaagctgcg gaactgccgc
     1081 gccaggctga ccgcggtgcc gaccgacatg ccgctgtcga tggtcagctg gtgcacgacc
     1141 gcgccgatga actggttggc cttgaggggg ttggtgagcc cctcggagag gcccttcttg
     1201 aggaccaccc gcaggaaggt ctgctggcgc tggatgcggc cgaggtcgct gagcgggtcg
     1261 tcgtgccagc gcccgttctt gaggtactgg tagtggcgcg agcgggcgac cgacagcgcc
     1321 tggtcgccgc tcaggtggag gcagccggtc ctggtgatgt tcagaccgga gaggttgtgg
     1381 ctgccgtccc agtccctgac agggaagggg aagtcgaggt tgatcccgcc gagcgcgttg
     1441 atgatcgagc ggaacccgtc gaagttgacc agcagatagt ggttgatggg gatgccgaag
     1501 tcctgctgga tcgtttcgat gagctgctcg gggcccttgt cgaaagcggc gttgatcttg
     1561 ttgcgcccgc tggtcccggc gatcgggacc agcgtgtcgc gcgggatcga caggatcttg
     1621 atctggtggg cggccgggac gatgcgcacc agcatgatga cgtcggagcg ctggccgctg
     1681 gcgtcctggc tgctgccgaa ctcggccttg tcgccggcct gggtcagggc gctgcgggag
     1741 tcggaaccca ccaccagcag caccatcggc ttgccgggct gggccgcctt gtgcaggccg
     1801 ggcaggttga tggaggcgat ctgcccgaag cgccagtgcg cgtacccgta gccgcccacg
     1861 gccacgacca gcgcgaccac cagcacgctg acgagcgcga tgatcagccg gcgctggcgg
     1921 cgctggtgct gctggcgttc acgcgacggc gcggcacgcc ggatcgcccg ccggacctcc
     1981 ggcgggagcg accccgtgcc tcgtcccgca ccgccgccac cttggccgcg gggccactca
     2041 tccacgaccg gccctcaccc cacctcgcta tggataccgg acatggtgga gcctgccacc
     2101 taaagtttgg atgaggaagc caagccgacc tcctccagtt ggctcagccc atgccgacgc
     2161 cctgggagcg ctgcaggctc ttgccctcgg tctggagggc gggggcgacc atcacctcgg
     2221 ccttctggaa ctccttgaga gtctggtagc cgcaggtggc catcgaggtc cgcagcgcgc
     2281 cgaacaggtt catggtgccg tcgttgaccg gcgcggggcc gagcaggatc tccttgaggg
     2341 tgccaagcgc ggacgtcttc acccgcgttc cgcgcggcag gtccggatgg aaggtggcca
     2401 tgccccagtg gtagccgcgg ccgggtgcct ccaccgcgcg cgccagcggc gagccgatca
     2461 tcaccgcgtc ggcgccgcag gcgatcgcct tggccacgtc gccgccggtg cgcatgccgc
     2521 cgtcggcgat cacctggacg tagcggccgg tctcctccag gtgccgcacc cgcgccccgg
     2581 ccgcgtcggc cagggcggtc gcctgcggca cgccgatccc gagcaccccc cggctggtgc
     2641 aggccgctcc ggggcccacg ccgaccagca cgccggccgc gccggtgcgc atcaggtgca
     2701 gcgcggtcga gtaggaggcg cagcccccca cgatcaccgg caggtcgtag cgggcgatga
     2761 aggtcttgag gttgagcggc tcggtggtct gcgagacgtg ctcggccgag acgaccgtgc
     2821 cctggatgac cagcacgtcc aggccggcgt cgagggcgtg ctggtggtag cgggcgaccc
     2881 gctgcggggt cagagagccg gctgccagcg cgccggcgtc cttgatctcc ttgacccgct
     2941 ggccgatcag ctctggtttg atcggctcgc ggtagatcgc ctgcatccgc ttggtcgcct
     3001 cggcgtcggg gaggccggcg atctcctggt agaggatgtc cgggtcctcg tagcgggtcc
     3061 agaggccctc catgttgagc accccgaggc cgccgaggcg gcccagctcg atcgcggtgc
     3121 ggggcgagac caccgcgtcc atcgccgagg cgagcagggg cagcgcgagc tggtaggcgt
     3181 cgaggtccca ggagatgtcg atgtcgtcag ggtcgcgggt gcgccggctc ggcacgatgg
     3241 cgatgtcgtc gaagccgtag gcgcggcgac cgctcttgcc gatgccgatc tcgacctcag
     3301 ccacgggatc ctccaggttt gggagagaac gggggaaaac caagaaaaca aaatgacccg
     3361 gcccgctggt cggccaggtc agatcgggac tccatacctg aagctaccag cgcggttttc
     3421 ccccggtcaa ggacccctca cgagccaccc gaagggggcc acgtgacgcg gaagcgcggt
     3481 accattccac cgtcaggacc agcgtcaccc gactgcttgc aagaggttca cgtgcccgac
     3541 ccacttgacc cgttcgggtc tgaagatgag agcgagagct cggatttcga gccgatcgac
     3601 gaggaggagc gcaccgcgct cctggaagac ctccgcgacc tggccgagtt ccgtgaggcg
     3661 ctcgaaccac gcggcttccg tggcgtcgtg atcccctgcc ccgactgcga ggaggaccac
     3721 tacttcagct ggagcctcct gcgcgagaac ctggagcaca tcctcgacca cggcgagcct
     3781 cgcgtccacg agccggcctt cgagccgcag gtcgaccact acgtgacctg ggactacggc
     3841 cgcggcttcg tcgacggcct gctggagggc gaacgggaga ccgcctccac cgcgctgcgc
     3901 gacgaatgga cctccccggt cgaggcgtcc cggcgcctgc accgcgccct gctcacccgc
     3961 ggcctcaccg aggacgaagt cgccgcggtc ctcaatgagg gcggcctgcc ccccgccgag
     4021 tagccggcgc ccgcgccctc tccatcttcg gctacatccg catcgggacg gggacccgca
     4081 ggtagccgcg gagctctccc agctcgtcgt cccagcccca ttcgttgtcg aggtaggcgc
     4141 gctcgcggac ctcctcggcc agcccggcga agccggactc ggtcagggtg agccgggtgc
     4201 cccgggggac gtcctcaagg gcgaactgga ccagcgtggt ggccgggtcg tcgccggccg
     4261 gccgccagcg gaaggcgagg cggcgccggg cctcgaccct ctcgatctcg gcgctggccc
     4321 gctcgccggc gccgaagtcg aaccgcaccg cgccgcccgg ccgcaggtcc agctcggcgc
     4381 tgttgcagaa ccagccggcg agctggtcgg ggtcggtcag cgcctgccac accagcttgc
     4441 ggggcaccgg caggaccatc tgccgccgca gcttgtcggg gaccttcgcc ttcaccccca
     4501 ctggcgctcc cggcccaggc cccggtcgtg gcggcggttg cgcaggcggt cgcgccagga
     4561 ggcgacggcc gccgcgaccg ccacgccgct ggccgccacc gccaccccgg acggcagcca
     4621 caccagccag agccggcggt ggcggcggaa gtcctgcacc ggccagccgc gttcggcggc
     4681 gagctggcgc agcatccggt cggggttgac cgcgaccgga tggccgacgc cctccagcag
     4741 cggccggtcg ttgaccgagt cggagtaggc gctggaggcc gccaggtcga tgccacgctc
     4801 cgcggccagg gcggtcaccg cctcgacctt ggcggggccg tgcagtaccg gtccgagcag
     4861 ccggccggtg tagacgccgt ccgcgagctc cgcctcggtg ccgagcgcgc cgtccatgcc
     4921 gaggtagcgg gcgatctcgc cggccaactc gcgcggcgcg gcggtgacca gccaggtgac
     4981 gtcgcccgcc gcgtggtggg cgtcgaccac ccggcgcatc cccgggtaga tgcgcgggcc
     5041 gatcacctcc accacgatgt cgtgcccgag ctgggtcagc tcgtcccgcc gcttgccctc
     5101 gacgaaggcc agcgccgact cgcgcgcggc ctccatgccg ccacggccct ccgcgccggt
     5161 gagccggaag gtgaactgcc cgtaggcgaa cccggcgacg tcccgccagc tgtagaaccc
     5221 gcgccggtag agcccgcgcg cgaacaggta caggctggac ccgggaagca gggtcttgtc
     5281 gacgtcgaag aacgccgcct tcgcctgttc tgccggccgt ggcgtggtca tttcggctgg
     5341 tcaaggccta gttgggtgcg gaaccacggc agcgtgcgct cgatcccctc ggcgaggctg
     5401 acctgcggct tccagccgag ccggctggtg gccagggtga tgtcgggccg ccgcacctcg
     5461 gggtcgtcct ccggccgggg cttgaagacg atgcgcgggt cgtcctgccc cgccgcccgc
     5521 gccaccagct tggccaactc cagcacggtg acctcctcgg ggttgccgat gttgaccggc
     5581 ccgctgaggt cgctccacaa cagccgccag atgccctcca ccaggtcgtc cacgtagcac
     5641 agcgagcgcg tctggctgcc gtccccgtgc accgtcagcg gctccccccg cagtgcctgc
     5701 accgcgaagg tgcagaacgc ccggccgtcg tcgattctca tgaaaggtcc gaaagtgttg
     5761 aaaatacgca caatgcgtgt atccagccca tgcgagcggt ggtaggccat ggtgagggct
     5821 tcggcgaagc gcttggcttc gtcgtagacg ccgcgggggc cgatcgggtt gacatggccc
     5881 cagtagatct cgggttgggg gtggacctgg gggtcgccgt agacctcgct ggtggaggcg
     5941 agcaggaagc gggcgcgttt gtccttggcc aggccaaggg cgcggtaggt gccgagggcg
     6001 ccgaccttga gggtctggat cgggtagcgg aggtagtcga tcgggctggc cggcgaggcg
     6061 aagtggagca ccacgtcgac cgggccggcg acgtggatga agttggtcac gtccacgtcg
     6121 atcaggcgga agcccgggcc ctgcagctcg tccacgttgg cggcacggcc ggtcagcaga
     6181 gagtccatgc agaccacgtc gaagccctcg gccaggaggc ggcggcagag gttggagccg
     6241 aggaagccgg cgcccccggt gatgaccgcc cgcggcacca gctaccccgc ccggtgggcg
     6301 tggtgggcgt cggaggcggg accggcgggc ggccggccga ccccggcgac ggtgaacccg
     6361 gcggcgacga aggcggcctg gtcgagggcg ttgcgggcgt cgaccaggat cgggtaggcc
     6421 atgcggccgg ccaggtcggc cgggtcaagc tcggcgaact cgccccattc ggtcatcagt
     6481 accagcgcgt gggcgccgat ggccacgtcg gcggccttgg cggccagctc gaccccgggg
     6541 gcgagctggg tgacctcggc gctgctggcg accgggtcgt aggcaagcac gctggcgccc
     6601 tcgtcgagca gcatcctggc cacgtcgatg gcgggcgact cgcgcaggtc gtcggtgtcg
     6661 ggcttgaagc tcagcccgag gaccccgatg tgcttgcctt ccaggtgcca cagcaggtgg
     6721 cggaccttct cgacgatcgc gcggcgggcg tcggagttga cccgggcgac ctcgcgcagg
     6781 atggcgaagt cgacgccgag ctcctgggag cggtgggcga aggcggcgac gtccttgggg
     6841 aagcagctgc cgccgtagcc gggcccggcg cgcaggaagg cggcgccgat gcgcgggtcg
     6901 aggcccatgc cgcgggcgac cagctcgacg tcggctccgg cgcgctcgca gacccgggcg
     6961 acctggttga tgaagctgat cttggtggcc aggaaggcgt tggaggcgtg cttgatcagc
     7021 tcagcggtgt tgcggtcggt ggccaggaac tcggtgccgg gccgctcggt gatcggggcg
     7081 tagagctgcc gcagcacctc ggtgccgcgt tgggactcgg tgccgaccac gatccggtcg
     7141 ggccgcaggg tgtcctcgac cgcggagccc tcacgcagga actcgggatt ggaggcgacc
     7201 tcgtagtcga cgccttcggg cgcctcgcgg gcgatcacct gggcgacccg ctcgccggtc
     7261 tgcaccggga cggttgattt ctcgcagatc agccggaatt cgccaaccgg caggttgcgg
     7321 gcgacctcgc gggcgaccgc ctcgacgaag cggaggttgg ggctgccgtc ggggcgcggc
     7381 ggggtcccga ccgagacgaa gatcacctcg ccgtgacgca ccgcctcgcc cttgtcggag
     7441 gtgaagcgga gccgcccgtt ggccagcgcc tcggccatca gcggctccag gtccggctcg
     7501 tagaaccagg ccttgccgct gcgcatcagg tcgatcttgg cctggtcgtc gtcgtcggcg
     7561 accacctcgt ggccgatctt cgccaggcac accgcggtga caagcccgac gtggcccgcc
     7621 ccgatcaccg tcacccgcat gtcagtcgct cccgtcgcgg tcctcgtagt gggcgatctc
     7681 taaagactca ggaaacagac ggcgatctta ccaatgcgtc gatccctgac gagcttcatc
     7741 cccatcttcc cggaactcgg gaacgtctcg atcacggtgc gtactcgaat gcgtaagcga
     7801 acccagatgc gtcatcgcgt ccgcctggcc gcggtcgcca ccgccctcgc ggccgccctc
     7861 gccgccggtc tggcgccggg tccggccttc gcggccacgg ccacggctgc cgccgccggg
     7921 ccgagcgtcg cgggacgttc ggcgatcctg gtggatgccg ccagcggcgc gatcctgtgg
     7981 agccgcgacc cggccacgcc gcgggcgccg gcgagcctca ccaagatgct gaccgcgctg
     8041 ctggtccgcg ccaccctgcc gctggacgcg gtggcggtgg ccggcaagga cgcggccacg
     8101 gccgcgccca cccggctggg gctgcgtgcc ggccagaagc tccgggtcgg gccggccctg
     8161 gaggcgatga tgatcctgtc ggccaacgac gtggcggtgc tgctcgccga ccgggccggc
     8221 ggctcaccgc agcggttcca ggcggcgatg aacgccgagg cggcgctgct cgggctgcgc
     8281 tcctcggcct ggcacagccc caacgggctg gacaaccccg ggcaccaggc cagcgcctac
     8341 gacctggcga tcctcggccg cgcggtgctg cgcgacccgt ggctcgcccg ggtcgtgcgg
     8401 atgcgccaga ccaccatcac tggcccggat ggccaccctc gcggcctcca tacccattcg
     8461 gggttcctgg tgagctaccc cggcgcgatc ggcatcaaga cgggcttcac gaacggcgcg
     8521 gggcactgcc tggccgccgc cgcgacccgc cacggccgca ccctgatcgc ggtggtgctc
     8581 gacagcccca gccccccggc cgacgcggcg cagctgatga actggggctt cgccggcggc
     8641 agctccggca gctccggcgg ctccagccgc cgggtctccg cggcgaccgc cgccgtggcg
     8701 atcctgccgc cgtatgtcca gccggtgggg gtgaactcgc tggtccctcc tgccacggtc
     8761 ccggctgcct cgctggtgac gccggcggtg ccccggggcc cgggtggcgg ctgcccaggc
     8821 actgccgctc ggccgcttcg ccggcgaggc cgcggttctg tgcggcctgc tgctggtcgg
     8881 cgctgccgtg tcgctcacca gccggcggcg ccggcggcgg cgctacctct tcagcgacgc
     8941 gcctggcgac accacgacca gctccagcct ctgagccgct tcaggcggtg gtgagctggg
     9001 cgcgcagctc ggcggctgcg gccgccgggt cgggggcgtc ggtgatggcg cgcaccacca
     9061 cggcgcgggt ggcgccggcg gcgctgaccg cgggcagagt cgcccggtcg atgccgccga
     9121 tcgcgaacca ggggaccggc gggtggagcg cggcgaccgc ctccagcagg ccggtcccga
     9181 cggcggggcg gccgggcttg gtggcggtgg catggaccgg gccgaccgcg aagtagtccc
     9241 agccctcctc gatcgcccgg cgggcctggc cgaggtcgtg ggtggagcgg ccgagcagca
     9301 ggccgtcacc gccgacgatc cgctccgccc aagccatcgg gaggtcgtcc tggccgaggt
     9361 ggaggatgtc ggcaccggcg gccagggcga cgtcggcgcg gtcgttgacc gccagcagcc
     9421 cgccgtggcg gtcggcggcc tcgcgcagca cggcggcggc ccgcagcagg ggcacggcct
     9481 cggcccgctt gtcgcggagc tgcaccaggt cgaccccagc gccgtagatc gcgtcggcga
     9541 aggcggccag gtccggccgg atcggcgtgc agacgtacag ccgcgcggcc gccagccgct
     9601 cccggcgggc ctgccgccgg tcagcggccc gatcgtcggg gtcagcggcc atctggctcg
     9661 gggagcggcg ctgacaggtc ggcgaggccc tcgaaggagc tggaggcctc ggcgtagagg
     9721 cgccggggga tcctgccggc gaggtgggcg aggcggccgg cctcgaccgc cttgcgcatc
     9781 gcctcggcca tcagggccgg ctcccgcgcg cgggtgaccg cgctggccag cagcaccgcg
     9841 tcgcagccga gctccatggc gacggcggcg tcggaggcag tgccgatgcc ggcgtcgagc
     9901 acgatcggga cggtcgcgtg ttcgaggatg atgcgaaggt tgtagggatt gcggatgccc
     9961 atgccgctgc cgatcggcga gccgagcggc atcaccgcag cgcagccgag cccctggagc
    10021 tggcgggcga ggatcgggtc gtcgttgctg taggcgagca cggtgaagcc gtcgtcgacc
    10081 agcgtctcgg ccgcgtcgag cagcccggcg ccgtccggca gcaggatgcg gtcgtcgccg
    10141 atcacctcca gcttgatcca gtcggtctcg aacgcctccc gcgccagccg cgcggtgcgc
    10201 accgcgtccc gggcggtgaa gcacccggcg gtgttgggca gcagcttgca gccaacccgc
    10261 cgcagcacgt ccagcaccga cccccgggcg tccgggctgg tccgccgcag cgccaccgtg
    10321 gccagctcgg ccccggacgc caccagcgcc cgctccagcg cctccatgct cgacgctccc
    10381 ccggttccca tgatcagccg ggacgagaac gcctgcccgg cgatggtgag cgggtcatcc
    10441 atggttctag cctccctgcg ccgcgccgag cacctcgacg cggtcctgct cgccgagcgt
    10501 ggtggtggtc caggcgcctt tggggaccac ttcgccgttg agggcgaccg cgatcccagt
    10561 cgcggtgggt gtgtggccgg ccacgccgag cgcggcggcg acggtcgcgc ccgggtccag
    10621 ctcggtcggg acgccgttga cgagcacggt catggctgcc cccctcccgt ctcggccgca
    10681 gccgccgtgg ccggcgccgc cgtggccgcc gccgcgccga agcgcagcgg cgagaacggg
    10741 gcgatcagct cggggacctg gccggtggcg agcagctcgg caatcgcgtc ggcggtcacc
    10801 ggggtcagca ggatgccgtt gcggtagtgc ccggtggcga cgaccaggcc gtcgctgagc
    10861 tggccgagca gcggcgcgtt gtccggggag ccgggccgca ggccggcgac ggtctcgatc
    10921 agccgcagct cgccggtgtc gggcagcagt tccagggccg cgcggagcag gtcgtggacg
    10981 gcgcggacgg tgacggtggc gtcgaagccc cgctcctcca cggtggcgcc gaccaccacc
    11041 cggccgtcgc cgcgaggcac cacgtagacg tccaggccgc ggacgttgtg gcggcacacc
    11101 ggcgcttctt gtggtccgcg caggtgcagc agctgcccct tgaccggccg gacggggggt
    11161 accagatccg ctgggacgcc ctccagcccg gcgctccagc agcccgccgc gagcagcacc
    11221 gttccagcgt cgatgcgctc gccggtggcg agcgtgacgc ctcgggcccg gcggggcccc
    11281 acgccctcaa gatgaaccgt ggccacgcgg ccgggcgcca cgcggacccc ggcccgctcg
    11341 caggcggccc gcagcgcctc ggccacggcc cggttgtcga cctgcgcgtc gctggaggcg
    11401 aggatgccgc cgcggacccc gggtgcgagg cccggctcga gctggcggca ttctcggcct
    11461 cgcagccgct cggattccag tccgagccca cgctggaagc ggtagaggtc gtcgagggcg
    11521 gcgttgtcgt cgccgtcgcg ggcgacgatc agggtgccgt tgcggcggta gccgacctgg
    11581 cagccgctga gctcctccag ctcggcggcg aagcctggcc accgctcggc cgctgccagg
    11641 ttgaggcgca gcagcgcctg ctcgccgtaa tggacttcgc cgacgggcgc gagcatcccg
    11701 gccgccgccc aggaggcgcc gtgccccggc tggtcgtcca ccacggtcac ggcgagccca
    11761 cgcccggcgg cccgccaggc gatcccgagc ccgatcacac ccccgccgac gatgacgagg
    11821 tcaggagcgt gccccgttcc ggctggctcc cgcatcgttg atctcctccc tccgccggca
    11881 ttacccggag caggttcaaa cggtcggcgg cacaacgaat tagccgccct ctcagcccgg
    11941 tggcccggac tcccgtgcgc ttcccagcgt actcttcacc ccgagatggc cagcaaggtc
    12001 gatatctacg tggaacaggg cgtccgcgcg cacgcgggcg gcgccgtggt ggcgtggacc
    12061 ttctcgctgc cgcactg
//
LOCUS       DASWDC010000129         7130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1616108, whole genome shotgun sequence.
ACCESSION   DASWDC010000129 DASWDC010000000
VERSION     DASWDC010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616108"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VF995_04145"
     CDS             <1..1157
                     /locus_tag="VF995_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX9342792.1"
                     /translation="APRVRVLGSRHSFTDLADSAELLSLEGLPADVAVDHAAGTVAFS
                     GGLTYGELATALQAEGLALANLASLPHISVAGAVATATHGSGDANGNLATAVAGLELV
                     TASGELLTVARGDADFDGLVVGLGALGAVTRISLDVEPAYQVRQRVFEGLAWDALLEH
                     FDEITSAGYSVSVFTRWGEAIDQVWVKSRVTDAPVRVRGDLFGAIAATVQRHPVLGLD
                     PVNTTPQLGVPGLWSDRLPHWRMGFTPSTGEELQSEYLVPRRHAAAAIQALWALGEVV
                     RPVLQVSEIRTVAADRLWMSPQYGQDTIAFHFTWTPDQDAVERVLVDVEAALAPFAAR
                     PHWGKMFIADAAAIASLYARLPDFARLVMRLDPRGAFRNAWLDTRVLGRQ"
     gene            1238..2866
                     /locus_tag="VF995_04150"
     CDS             1238..2866
                     /locus_tag="VF995_04150"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9342793.1"
                     /translation="MAAAIDHVIVVALENRSFDQVLGFHPSSNPDFDGLLDGGPYQNP
                     GYDGGPQVDAASTGKHVLPIDPDHSHDAVMLQLGLTSGAATPTNAGFVHSYERKGRGL
                     AQPAFGGLLAPLLKLLHVGQSTGTATDLIAGRGPLIMRCLAPEHVPVLTELAASFGVC
                     TRWFASVPGETWPNRNFLHAATSDGTTDIEPRFYDNTTIFEVLEKVGKSWHVYYDDTP
                     QVWAFKRLWDEGRARNWFRSAQFAEHVRSGQLPNYSFIEPNHRPPVHATPFVHVAGVH
                     GHSNSQQPGNNLVPDTDYDAAAASGHGDFERGESLIAEVYEALRANPEVFNRSVLLVT
                     YDEHGGFFDHVPPPTGVPAPGGRLRPGLLGLLARSLLHRKAAVFDFTMLGVRVPAVVI
                     SPLVPAGSVDATVRDHASVPATLRALFAPDEPPLSERDRWSPPFLSLLSLRDPRTTDL
                     PDLSAWVAQEPGPAAAAPPPVPPPTPPAQPEPPVPESFSDLVGLARMVDRELPGPTAP
                     PQLGPRATARQVTEEFQGHAEAIRQQAPAEGGSP"
     gene            2863..3768
                     /locus_tag="VF995_04155"
     CDS             2863..3768
                     /locus_tag="VF995_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342794.1"
                     /translation="MTAAVIDVVVGLAFLYFLLSLVSSGVNELIAGVLRLRARNLELG
                     IGRLLGDEDLKRRLFDHPLIKPLGQPRGTLNGPGRKPSYIPANKFTLALLDLVAAPAT
                     GTGTVPEGVEAQVQEAIAGLPEDLRASLTLLWRRAAGDLDAFQAQVEGWFNEAMERVS
                     GWYRRKVRVLLIAIGIVIAVALNISTVTVASRLWTDAPLRAAVVAQASKAQLPATAGQ
                     QSIQDAARAVQTGIGSVTQLALPMGWNRQLRPAGSPLAWLAAVVGWLLTAAAVSLGAP
                     FWFDLLNKVANLRAGGAPPKDSNRT"
     gene            3885..4307
                     /locus_tag="VF995_04160"
     CDS             3885..4307
                     /locus_tag="VF995_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9342795.1"
                     /translation="MRPLQTIIVGYDETEPARRALDRAVQLARAFDAKLVVTSVAPTM
                     LGVGRSAGAIDPTDTPTDHRHQLAHARELLVQLGMDAELVPALGEPAHTIVELAEDRD
                     ADLIVVGTREPSVLARLLGQSVSSSVARQTHCDVLIVH"
     gene            complement(4335..4419)
                     /locus_tag="VF995_04165"
     tRNA            complement(4335..4419)
                     /locus_tag="VF995_04165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4384..4386),aa:Leu,seq:tag)
     gene            4977..5753
                     /locus_tag="VF995_04170"
     CDS             4977..5753
                     /locus_tag="VF995_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342796.1"
                     /translation="MTVLDQGKGRARQLVVVIGASGAAEVAWSAAGAAVLPGLPALYR
                     LDAEAGEGIMRPIDAPAGLVADPAPAPGSVVAPEGFTVTIAPASAPWQAGPVLHRVTY
                     SWSGTLPYDTYEWPDEVPVSFAEAVERCCLLVRRARCLATRRLVEPGARRRAEAWGEN
                     VLLHQLDPWQRAQYEAEQRFLVQAPSGRHYLITRQRCGNVYALDGLGRSTAAWSLLPA
                     VPVSLAHQLGQQAWLLAHDEERFLRFALAAQFEEGGERVP"
     gene            5804..6994
                     /locus_tag="VF995_04175"
     CDS             5804..6994
                     /locus_tag="VF995_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX9342797.1"
                     /translation="MNGLLIEEEPAPASAALVLPAPRPGLAFELPAVLRAGAPPESRG
                     LARDEVRLMVSEGSRAPLSTSFRDLPRHLRPGDLLVINTSATMAALLDATTPGGPGGR
                     RVEVELRLVTPLPPHGLWVVELPATAMRRPDASGGPAATLASARGSFGRGTVPPEPPD
                     ASGGGGGALPLAGGGYLQLLAPYGNKLRAVEQPRLWIGALHLPAQLGAYLHAYGRPAR
                     PPGVTDDWPLSVYQTCFARQPGSTAMASAGRAFTPELLCALLAGGIAVAPLCLHTCGV
                     ADDDAPLPEPYEVPAATARLVNATRAAGGRVVAVGTTVVRALETVTDSQGQAHPAQGL
                     TDTVVTPEHGVRGCDGLLTGWHEPNGSHLHRWRRSPGWSRWTAPTAPHSTAAMPGALS
                     ATST"
BASE COUNT          979 a         2597 c         2505 g         1049 t
ORIGIN      
        1 acgcaccccg ggtgcgggtg ctcggctcgc ggcactcgtt caccgacctg gccgactccg
       61 cggagctgct cagcttggag ggcctgcccg cggacgtcgc tgtcgaccac gcggccggga
      121 cggtcgcctt ctctgggggc ctgacctacg gcgagcttgc cacggcgttg caggccgagg
      181 ggttggccct ggccaacctg gcgtcgctgc cgcacatttc ggtcgcgggt gcggtcgcga
      241 ccgccaccca tggctccggc gacgccaacg gcaacctcgc gacggcggtc gcggggttgg
      301 agttggtcac cgcctcgggc gagctgctca ccgtcgcaag aggcgacgcc gacttcgacg
      361 gcctggtcgt cgggctcggc gcgctcggtg cggtcacccg tatcagcctg gacgttgaac
      421 ccgcctatca ggtgcgccag cgggtgttcg aaggactcgc ctgggacgcc ctgcttgagc
      481 acttcgacga gatcacctcg gctggctaca gcgtgagcgt gttcacccgc tggggcgagg
      541 cgatcgacca ggtctgggtg aaaagccggg tgaccgatgc tcctgtgcgg gttcgtggcg
      601 acctgttcgg tgcgatcgct gcgaccgtcc agcggcaccc ggtcctcggc ctcgacccgg
      661 tcaacaccac accgcagctc ggcgtgcccg gcctgtggtc cgaccgcctt ccgcactggc
      721 gcatggggtt cacgccgagc accggcgagg aactccagtc ggagtacctg gtgccgcgcc
      781 gccacgccgc tgcggcgatc caggcgctgt gggcgctcgg cgaggtggtc cggcccgtgt
      841 tgcaggtgtc cgagatccgc accgtcgccg ccgaccggtt gtggatgagc ccacagtatg
      901 gacaggacac gatcgcattc cacttcacct ggacgcctga ccaggacgct gtggagcgcg
      961 tgctcgtcga cgtcgaggcc gcgctggcgc cgttcgccgc gcgcccgcac tggggcaaga
     1021 tgttcatcgc ggatgcggcg gcgatcgctt cgctgtatgc gcgcctgccc gacttcgccc
     1081 gcctggtcat gcggctcgat ccgcgcggcg cgttccgcaa cgcctggctg gatacccgcg
     1141 tgctcggccg ccaatagccc cctaccaact cgacgctcgc gcggattggg actttaatgc
     1201 caggcggtac ccggctgcgg tcgatcgggg gaagcccatg gcagctgcaa tcgaccatgt
     1261 catcgtggtc gcactggaaa accgctcgtt cgaccaggtc ctcggcttcc atcccagcag
     1321 caacccggac ttcgacgggt tgctggacgg tggcccctac cagaacccgg gctacgacgg
     1381 cggcccacag gtcgacgccg catcaaccgg caagcacgtc ctccccatcg accccgacca
     1441 ctcccacgac gcggtcatgc tccaactcgg gctcacaagc ggcgctgcca ccccgacgaa
     1501 cgccggcttt gtccacagct acgagcgcaa aggcaggggt ctcgcccagc ccgcgtttgg
     1561 cgggctgctc gcacccttgc tgaagctgtt gcacgtcgga cagtccaccg gcaccgcgac
     1621 cgatctcatc gctggccgcg ggccgctgat catgcgctgt ctggcgcccg agcacgtccc
     1681 ggtgctgacc gagctggcgg ccagcttcgg cgtgtgcacc cgctggttcg cctcggtgcc
     1741 gggcgagacc tggcccaacc gcaacttcct gcacgcggcg acctccgacg gcaccaccga
     1801 catcgagccg cgcttctacg acaacacgac catcttcgag gtgctggaga aggtcggcaa
     1861 gagctggcac gtctactacg acgacactcc ccaggtgtgg gcgttcaagc gcctgtggga
     1921 cgagggccgc gctcgcaact ggttccgttc ggcgcagttc gccgagcatg tgcgctccgg
     1981 tcagttgccc aactactcgt tcatcgagcc gaaccaccga ccgcccgtgc acgccacccc
     2041 gttcgtccac gtcgccggcg tgcatggcca cagcaacagc cagcaacccg gcaacaacct
     2101 ggtccccgac accgactacg acgccgccgc ggcgagcggg cacggcgact tcgaacgcgg
     2161 cgagtcgctg atcgccgagg tctacgaggc gctccgcgcc aatcccgagg tgttcaaccg
     2221 ctcggtgctg ctcgtcacct acgatgagca cggcggattc ttcgaccacg tgccgccgcc
     2281 caccggcgtg ccagcgccgg gtggccggct acggcccgga ctgctcgggt tgctggcccg
     2341 ctccctgctc caccgcaagg ccgcggtgtt cgacttcacc atgctcgggg tgcgggtccc
     2401 agccgttgtg atctcgccgt tggtgccagc cggctcggtc gacgcgaccg tccgcgacca
     2461 tgccagcgtg ccggccacgc tgcgggcgct gttcgccccc gacgagccac cgctcagcga
     2521 acgcgaccgc tggtcgccgc cgttcctctc cttgctgtcg ctgcgggacc cacggacgac
     2581 cgaccttccc gacctgtccg cgtgggtcgc ccaggagccc ggacccgccg cggccgcgcc
     2641 gccgccggtc cctccgccca ccccgcccgc gcagcccgaa ccgccggtcc ccgaatcctt
     2701 cagcgacctg gtcggcctcg ccaggatggt tgaccgcgag cttcccggcc cgacggcccc
     2761 accgcaactg gggccacgcg ccacggcgcg ccaggtcacc gaggagttcc aaggccacgc
     2821 cgaggccatc cgccagcaag cccccgcaga gggggggtcg ccgtgaccgc ggccgtcatc
     2881 gacgtcgtcg tcggactcgc gttcctgtac ttcctgctca gcctggtgtc ctcgggggtc
     2941 aacgagctga tcgccggagt gctgcggctg cgggcacgca acctcgaact cgggatcggc
     3001 cgcctgctcg gcgacgagga cctgaagcgc cgcctgttcg atcatccact catcaagccg
     3061 ctcggccaac ctcgggggac gctcaatggg cctggacgca agccctcgta tattcccgcg
     3121 aacaagttca ccctcgcact gcttgacctg gtggcagcac ctgcgacggg taccgggacg
     3181 gtcccagagg gcgtcgaggc gcaagtccag gaggcgatcg ctgggctccc ggaagacctt
     3241 cgggcgtcgc tcaccttgct gtggcggcgc gcggcagggg atctcgacgc gtttcaggcg
     3301 caggtcgagg gatggttcaa cgaggcgatg gagcgggtct cgggctggta tcggcgcaag
     3361 gtccgggtgc tgctgatcgc gatcggcatc gtgatcgcgg tcgccctcaa catcagcacc
     3421 gtgaccgtcg caagccgcct ctggaccgac gccccgctgc gggcggcggt ggtcgcccag
     3481 gcaagcaagg cacagctccc ggcaaccgcg ggccagcagt cgattcaaga cgccgcacgg
     3541 gcggtgcaga ccgggatcgg cagcgtgacg cagctcgccc tgccgatggg atggaaccgg
     3601 cagcttcggc cggcgggctc cccgctggcc tggctcgctg cggtggtcgg ttggctgctc
     3661 accgcggcgg cggtgtccct tggtgcgccc ttctggtttg acctgctgaa caaggtggcc
     3721 aaccttcgcg ctggtggtgc gccgcccaag gacagcaaca ggacgtgacg gcagcgcccg
     3781 cgactggtcg cccggcgccg tgattgacgg cgcgtgggct cgggaatgct gctcatggag
     3841 cccgcgaccg gtcgcgggcg acggcggctg gttgggaggt tgcgatgcga ccgctacaga
     3901 cgatcatcgt tggctacgac gagaccgaac ccgcccgccg ggcgctggac cgtgccgtcc
     3961 agctcgcccg cgccttcgac gccaagctcg tggtgaccag cgtggccccg acgatgcttg
     4021 gcgtcggccg ctcggccggg gcgatcgacc ccaccgacac gcccaccgac caccgccacc
     4081 agctcgccca cgcccgcgag ctgctggtcc agcttgggat ggacgctgag ctggtgccgg
     4141 cgctggggga gccggcacac accatcgtcg agctcgccga ggaccgcgac gccgacctga
     4201 tcgtggtcgg cacccgcgag ccgagcgtgc ttgcgcgcct gctgggtcag agcgtcagca
     4261 gctcggttgc ccgccagacc cactgcgacg tcctgatcgt gcactgacgg caacagatcg
     4321 cctgctcgac cgagtggtgc gagcggtggg acttgaaccc acacggccaa gggccaccgg
     4381 gtcctaagcc cggcgcgtct gccattccgc cacgctcgcg tggtgcgcat gagtggtgcg
     4441 cttgagtgag ccgcacaagg gtagcgcagg cggctgggtg ggacgggatg ggccatcttc
     4501 ctgccgagat tgattggccc agttgtgcta gtcaggcggc ctgccgtggc gggtatggcg
     4561 gagtggctgg tcaggggttt gttggcgaca tcaaacttgc ttattcagct ttagcgtgac
     4621 gttgagagcc aacttgacga aaaggcgcag tcgactcgat actctgcatc gtcacatggt
     4681 tcattggggg gccagggatg gaccagctgc agcatgcggc cgttgccgag gtacgacgcg
     4741 cggcgcggcc ccacctcact ctgcgagggc tcgtgcgagc gccgcgctcc cctgtcgttg
     4801 gttggtacac cgtcccggag cactagcgct acctcgagaa catccgcccc tgctccagtg
     4861 acgagcagcg gcgtcacagc cataccacat gcggcaggac ggcagcgtcg ccgatcagac
     4921 agccatcggc ggcggccggg cgggcacgcc catgcccgcg aatcagaggg gggaacatga
     4981 ccgtgctcga ccaggggaag ggccgcgcgc ggcagttggt cgtcgtcatc ggcgcgagcg
     5041 gggcggccga ggtcgcctgg agcgcggccg gggcggcggt gctgcccggc ctgccggcgc
     5101 tctaccggct ggacgccgag gccggcgagg gcatcatgcg gccgatcgac gcgccggccg
     5161 gcctcgtggc cgacccggcg ccagcgccgg gctcggtggt ggcgccggag ggattcacgg
     5221 tcaccatcgc gccggcctcg gcgccgtggc aggccggccc ggtgctgcac cgggtcacct
     5281 acagctggtc gggcaccctg ccctacgaca cctacgagtg gcccgacgag gtcccggtca
     5341 gcttcgccga ggcggtggag cgctgctgcc tgctggtgcg tcgggcacgg tgcctggcga
     5401 cccggcggct ggtcgagccg ggcgcccggc ggcgcgccga ggcgtggggg gagaacgtcc
     5461 tgctgcacca gctcgacccg tggcagcgcg cccagtacga ggcggaacag cgcttcctgg
     5521 tccaggcgcc gagcggccgg cactacctga tcacccggca gcgctgcggc aacgtctacg
     5581 cgctcgacgg cctcggacgg agcacagcgg cctggtcgct gctgccagcc gtgccggtca
     5641 gcctcgccca ccagctcggg cagcaggcgt ggctgctggc ccacgacgag gagcggttcc
     5701 tgcgcttcgc cctggcagcg cagttcgagg aaggcgggga gcgagttcca tgagaagcac
     5761 gcggagcgca tcaggagtgg tgcatgacga tggcggtgcg tgaatgaacg ggttgctgat
     5821 tgaggaggag ccggctcccg cctccgccgc cttggtgctg ccggcgccga ggccggggct
     5881 cgccttcgag ctgccggcgg tgctgcgggc gggcgcgccg ccggagtccc gggggctcgc
     5941 ccgggacgag gtgcggctga tggtctcgga ggggagccgg gcgccgctct ccaccagctt
     6001 ccgggacctg ccgcgccacc tgcggccagg ggacctgctg gtgatcaaca cctcggcgac
     6061 catggcggcg ctgctcgacg ccaccacgcc cggcggcccc ggaggccggc gggtcgaggt
     6121 ggagctacgc ctggtgacgc cgctcccgcc gcacgggctc tgggtggtcg agctgcccgc
     6181 cacggccatg cggcgccccg acgcatcggg gggccccgcg gccacgttgg catcggctcg
     6241 cggttctttt ggccgcggga ccgtgccccc cgaacccccc gacgcatcgg ggggcggcgg
     6301 gggggcgttg ccgctggccg gaggcggcta cctccagctg ctcgccccct acggcaacaa
     6361 gctgcgggca gtggagcagc cccgcctgtg gatcggggcg ctgcacctgc cggcccagct
     6421 cggcgcctac ctccatgcgt atggacggcc cgcgcgccct ccaggggtca ccgacgactg
     6481 gccgctgagc gtctaccaga cctgcttcgc ccgccagccc gggtcgaccg cgatggcgag
     6541 cgccgggcgg gccttcaccc ccgagctgct gtgcgcgctg ctggccggcg gcatcgcggt
     6601 ggcgccgctg tgcctccaca cctgcggggt cgccgacgac gacgcgccgc tgccggagcc
     6661 ctacgaggtg cccgccgcca ccgcgcggct ggtgaacgcc acccgcgccg ccggcgggcg
     6721 cgtggtcgcg gtcggcacca cggtggtgcg cgccctggag acggtcaccg actcccaggg
     6781 ccaggcccac cccgcccaag gcctcaccga cacggtcgtc acccccgaac acggcgtgcg
     6841 cggctgcgac ggcctgctca ccggctggca cgagccgaac gggtcgcacc tgcaccgctg
     6901 gaggcgctcg ccgggctgga gccgctggac tgcgcctacc gcgccgcact cgaccgccgc
     6961 tatgcctggg gcgctttcgg cgacctccac ctgatcctgg ccgacagcgc cgccggcccc
     7021 acactcggtt ccgggactcc ctgtcagcga aaggggtact gagatgcgct accggactct
     7081 cgggcgggac ggcccggcac tgtcggtggc cggcttcggc gcctgggcga
//
LOCUS       DASWDC010000130         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_767172, whole genome shotgun sequence.
ACCESSION   DASWDC010000130 DASWDC010000000
VERSION     DASWDC010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767172"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VF995_04180"
     CDS             <1..342
                     /locus_tag="VF995_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9342798.1"
                     /translation="LAARKHHNAGQLSGGEQQMLAIARALMTNPRLLVMDEPSEGLAP
                     VIVESLGEHLQALRASALSILLVEQNYTLAMTLADTVYILENGRIVFHGPPATLDASD
                     DVKRRHLGVGV"
     gene            464..607
                     /locus_tag="VF995_04185"
     CDS             464..607
                     /locus_tag="VF995_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342799.1"
                     /translation="MLAPILVGGVLAILTFAGLQLFDRLHSDQYDETYEQALRERRSP
                     HTW"
     gene            complement(918..1400)
                     /locus_tag="VF995_04190"
     CDS             complement(918..1400)
                     /locus_tag="VF995_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9342800.1"
                     /translation="MIPVLNRTYDTQICSIARTLEVVGERWTLLVLRDVLRGTRRFEE
                     FVQSLGVARNVLSDRLDRLVAHGLVERKPYQDHPPRFEYHPTPKGSELLVVILGLMHW
                     GDRHLAGRAGPPRLAEHRDCGGAVVEQLTCTSCGQALTPGEITTRPGPGLREHDPAAV
                     "
     gene            complement(1429..3195)
                     /locus_tag="VF995_04195"
     CDS             complement(1429..3195)
                     /locus_tag="VF995_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9342801.1"
                     /translation="MTETETQTRQPLSRNLQLEALREALRWPNPNSLTVMALAGQLLA
                     ARQDEQGSAVFTELAEAAPDQPLYAALAGFFQVRAGHDLDHALRLLDRAASQAPGLAN
                     YFRGLVLANLPASLGKAETAVADLELVLAIPEQAGFPAGLRRVVYPALAAAYATLGRS
                     DDAEAARRQAGPAAAGPDGPALTTDWWVTRDDGFHLGPPRVVERAPGVFVAQGFDFAD
                     VVAIDTGAGVVLVDTTTSRAHLRAALAALRTASGSMAPITHVILTHAHTDHVGGLDEV
                     RAANPGVEVIAQVNFADELRLQHAAPAILFRLGETSGAGRWVDVVPDRLVGAPEKLAV
                     GGVELALYPIDGGETSDGLVVHLPDRGVAIVGDMLMPQLGAPFLPEGSAEGLFDAMTL
                     VEGLGPRLVIHGHTPLTELFTIEIFPSLRVALTELYQQTLAAIREGVPLAGILARNHL
                     PASLRDHPDAVLPYLVMRDNLIQRVQHQRTGYWKADGEGIEVFTATEWAAALDLLGGS
                     TAGAFTRAGGDLLDRGDVPLALRLVDYGRLRHPDDEGLRDLRRRVLSRLLERYQQLSP
                     FKFALYAATAGVEVPPAPAGRL"
     gene            3389..>3868
                     /locus_tag="VF995_04200"
     CDS             3389..>3868
                     /locus_tag="VF995_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342802.1"
                     /translation="MSTRVRPKSKAPTAPVGSAGRPVLLATMDVPFERQAWVVAVDAA
                     VESGQPLIVANMVVLPPLPLSVMMGYDQLDYTPELAKALREPAELAQSLGLRVERLRV
                     RSLHPIQALVELVRELRPGLLVFGPDRTKLPRRTYRRAARTLRQRIDCLIWLAPEAES
                     "
BASE COUNT          551 a         1335 c         1414 g          568 t
ORIGIN      
        1 ctcgccgccc gcaagcacca caacgccggc caactctccg gcggcgaaca gcagatgctc
       61 gccatcgccc gcgccctgat gaccaacccc cgcctgctgg tcatggacga gccgtccgaa
      121 ggcctcgccc ccgtgatcgt cgagtccctc ggcgagcact tgcaggccct gcgcgcctcc
      181 gccctgtcga tcctcctggt cgaacagaac tacacgttgg cgatgaccct cgccgacacc
      241 gtctacatcc tggagaacgg ccgcatcgtc ttccacggcc cccccgccac cctcgacgcc
      301 tccgacgacg tcaagcgccg ccacctcggc gtcggcgtct agcgcgtatc tgggtggagg
      361 ctgagctgat cggccgttta gcgtagttga cgagctgagt cgctcatgcg atggttacgg
      421 tggcagaccg tacccggacc tctctttgaa aggagctttt ttcatgcttg ccccgattct
      481 tgtgggcggc gtcctcgcca tcctcacctt cgccgggcta caactctttg accggttgca
      541 ttcggaccag tacgacgaga cctacgagca ggcactccgc gagcgccgct cgccgcacac
      601 ctggtagcgc tcccccggtc ccacaggcat gcccggtcgg gcatccaaat gcccggccgg
      661 gcactcccga aaggagtcgg cagaccgccc cggggacccg gccgggcact cactgatcat
      721 cgcatactgg acaagcccct tgcccacgaa ccccgccgca cccggcaagc gcgccgtcgc
      781 cgagttgccc gaagccgttc tggccctagg cgagcgcccc ttggggggtg gccgccgacg
      841 ccgggacccg agagcgcgat cgtcaggccg gtgcttcggc gctgatccgc acctcgcgtt
      901 ccgcacgcgg gtgtagctca gacggcggcg gggtcgtgtt ctcggaggcc cggtcctggg
      961 cgggtagtga tctcaccggg agtgagcgcc tggccgcaag acgtgcaggt gagttgctcg
     1021 acgaccgcgc cgccgcagtc gcggtgctcg gcgaggcggg gcgggccggc gcggccggcg
     1081 aggtggcggt cgccccagtg catcaggccg aggatgacca ccagcaactc gctgcccttg
     1141 ggggtggggt ggtactcgaa gcgtggcggg tggtcctggt agggcttgcg ttcgacgagg
     1201 ccgtgggcga ccaggcggtc gaggcggtcg ctgaggacgt tgcgggcgac gcccaggctc
     1261 tggacgaact cctcgaagcg gcgggtgccg cgcaggacgt cccggaggac gagcagggtc
     1321 cagcgttcgc ccacgacctc gagggtgcgg gcgatggagc agatctgagt gtcgtaggtt
     1381 cggttgagca cgggaatcat cgtatcccgg tcggtttcgt gacgctacct acaggcgacc
     1441 cgcgggcgcg ggcgggacct cgacgcctgc cgtggcggcg tagagggcga acttgaacgg
     1501 actgagctgc tggtagcgct ccaggagccg ggacaggacc cggcgccgca ggtcccgcag
     1561 gccctcgtcg tcggggtggc gcaaccggcc gtagtcgacc aggcgcagcg ccagcggcac
     1621 gtcgccgcgg tccagcaggt cgccgcccgc gcgggtgaag gcaccggcgg tcgaccctcc
     1681 aagcaggtcc agggcggcgg cccactcggt cgcggtaaag acctcgatcc cctccccgtc
     1741 ggccttccag tagccggtgc gctggtgctg gacccgctgg atgaggttgt cgcgcatcac
     1801 caggtagggc agcaccgcgt cagggtggtc gcggaggctg gccggcaggt ggttgcgtgc
     1861 caggatgccg gccagcggga caccctcccg gatggccgcc agggtctgct ggtacagctc
     1921 cgtgagggct acccgcaggg acgggaagat ctcgatggtg aacaattcgg tgagcggtgt
     1981 gtggccgtgg atgaccagac gtggccccag gccctccacc agggtcatgg cgtcgaacag
     2041 gccctcggcg gacccctccg gcaggaacgg cgcgccgagc tgcggcatga gcatgtcgcc
     2101 gacgatggcg acgccccggt cggggaggtg gacgacgagc ccgtcgctgg tctcccctcc
     2161 atcaatcggg tagagcgcca actccacgcc gccgacggcc agcttctccg gtgcgcccac
     2221 cagccggtcg gggacgacgt caacccagcg gcccgctccg gaagtttcgc ccagccggaa
     2281 gaggatcgcc ggggcggcgt gctggaggcg aagctcgtcg gcgaagttca cctgggcgat
     2341 cacctcgacg ccggggttgg cggcgcggac ctcgtcgagg ccgccgacgt ggtcggtatg
     2401 ggcgtgggtg agaatgacgt gggtgatcgg cgccatgctg ccgctggccg tccgcaacgc
     2461 ggcgagggcc gcgcgcaggt gcgcccggct ggtggtggtg tcgaccagca cgacccccgc
     2521 gccggtgtcg atggcgacga cgtcggcgaa gtcaaacccc tgcgcgacga acaccccggg
     2581 agcgcgctcg acgacccggg gcgggccgag gtggaagccg tcgtcgcggg tcacccacca
     2641 gtcggtggtg agcgccgggc cgtcggggcc ggcggcggcc ggcccggcct gacggcgggc
     2701 cgcctcggcg tcatcgctgc ggcccagcgt cgcgtaggcc gcggccaggg ccgggtagac
     2761 cacacggcgc agcccggcgg ggaagccggc ttgctccggg atggcgagca ccagctccaa
     2821 gtcagcaacg gccgtctcgg ccttgccgag agaggcgggg aggttggcca ggaccagccc
     2881 ccggaagtag ttggccaggc cgggcgcctg gctggctgcc cggtcgagca ggcgcagcgc
     2941 gtggtcgagg tcatggccgg cgcggacctg gaagaacccg gccagcgcgg cgtacagcgg
     3001 ctggtccggg gcggcctcgg cgagttcggt gaacacggcg ctgccctgct cgtcctgcct
     3061 ggccgcgagc agctggcccg ccagggccat taccgtcagg gaattcgggt tcggccaccg
     3121 cagcgcctcg cggagcgcct caagctgaag gttgcggctg agcggctggc gcgtctgggt
     3181 ctcggtctcg gtcatggtcg cgtcctccag ctccaaggtg agttgcttga cgaaactcta
     3241 tgtagcctgc aaggtttcgt caagcaactc gttcgagggc gtcgcgcgct gtggaaggga
     3301 ccgagccggc cgcggggttg atctcctctc ccggattgat gcgtgccaga ctcttgagcg
     3361 caggcacacg ctcccccttg gagggccgat gtcgacccgt gtccgcccga agtccaaggc
     3421 tcccaccgcg ccggtcgggt cggcgggacg gccggtgctg ctggccacca tggacgtccc
     3481 gttcgagcgg caggcctggg tggtggcggt ggacgccgcg gtggagtcgg ggcagccgct
     3541 gatcgtggcg aacatggtgg tgctcccgcc gctgccgctg tcggtgatga tgggctacga
     3601 ccagctcgac tacacccctg agctggccaa ggccctgcgc gagccggcgg agctggcaca
     3661 gtcgctgggc ctgcgggtcg aacgcctccg ggtcaggagc ctccacccga tccaggccct
     3721 ggtggagctg gtgcgcgagc tcaggcccgg cttgctggtg ttcgggcccg accggaccaa
     3781 gctcccccgc cgcacctacc gccgggcggc ccggaccctt cgccagcgga tcgactgcct
     3841 gatctggctg gcacccgaag ccgagtca
//
LOCUS       DASWDC010000131         6044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_280930, whole genome shotgun sequence.
ACCESSION   DASWDC010000131 DASWDC010000000
VERSION     DASWDC010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280930"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2259)
                     /locus_tag="VF995_04205"
     CDS             complement(<1..2259)
                     /locus_tag="VF995_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342803.1"
                     /translation="MKDQAKANDKAKRRKGKDAKGQSGDARRVIRLIRRFLAGQRRVV
                     RLALLMLVIESITAVAEPGPIAYLINFLKGGAHSGQFGSRNMTVALVTGAIVVLAAVN
                     SLCDSVAEINFARGGRMLGFNLRVKLYDHLQKLSLAFHNQGRTGDVLARVTSDVTALE
                     DFVIESSSDIIGSVFILVGTLGFLLYKSPQVALIALLIIPILASVSNFFTGRIKSASK
                     RQRAREGELASTAQEMLTSIRVIQIYGRGGREVQRFAKENQQAMNAALDAARVEATFS
                     WTVSVLEALCISGVVWMGLFLFDRQQLSLGLLVAFVLWIENMFKPTRRIIKEWNTLGK
                     VFASVERIGEVLDREPAVQDLPGAVPAPPLLGHLEFREVSFAYQAEPDAAKAKASANG
                     GAVGAGAGLRLALDHLSFSVRAGESVALIGHSGAGKSTVAQFAPRLYDPHDGQVLIDG
                     HDLREFTLESLRDQVTMVLQEAILFNGTVADNIAYGRDGATREEIVEAAKKANAHDFI
                     LQLPDGYDTELAERAQNLSGGQRQRISIARAFIRDTPLLILDEPTTGLDAESTDAVLE
                     ALRLLSGGKTTLVISHDMGLISDADRILVLEGGHISQEGTHQSLLAQGGLYADLYHRQ
                     YGAAEVERGLAESALAEHDRPLPMPASPPVKPPVPALTAAALDDDAAPAGVRRKVFET
                     VLMQALPLPASRQEFERMTMTHHAPPRPPTAPPLPPAETPPRPSTAPPRSPTAPPLPP
                     QPPPPSRQPSRRP"
     gene            complement(2256..3602)
                     /locus_tag="VF995_04210"
     CDS             complement(2256..3602)
                     /locus_tag="VF995_04210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9342804.1"
                     /translation="MKTSREPQRPATPALGVLVRGWPRLSQTFVLDEVLALERLGLRL
                     RIFALTSTSTEALTQPELTAVRAPVEYLDGGTPRRRTAVAAHLRALLASPPRYLATAW
                     YVARHPEWDRGYHAASRGRCLTLAATLAATLRRAGREGEGGAVGRLHAHFAHDPALVA
                     LLTHRLTGIPWSFTAHARDLFQVPPAAVADRVAAAEAVVTCCGPNADYLRDVLGDGLG
                     ERVRLIHHGVDVEAFQPATGHGPGRNREGDRDAARDAAAPRIVSVGRLVEKKGFADLL
                     AACHRLKQRGQRFHLELYGDGPLGEELAATVDRLGLGDEVVLAGSRTRPELRAALRRA
                     DLFALTPFVTADGDRDGIPNVLLEAMACGLPVVSTTVAGVTEVMRHGADGLLAPPRDV
                     DAIADHLAVLLADERLRARLGGQARRTVVERFDGRDAAATLAALLAPASVSAGGSS"
     gene            complement(3586..4620)
                     /locus_tag="VF995_04215"
     CDS             complement(3586..4620)
                     /locus_tag="VF995_04215"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX9342805.1"
                     /translation="MTSTSVGKHPTALHLTGLPILQVALNVQRMTDKLAPLLEALAGP
                     DGPPPVTYARLLAYKQGNRGTIRYQVATEGGPTTVLGKLYPQPGQAGRVDTILRGLAA
                     AFAATPRLGVPRPLGCLPDLAMLVYIPVEGEILDEVLQSSAGGAGAIERTADWLATLH
                     RARFELDRRFQPSLELVNLQAWGALISQAHPDLASTVAHLSDRLQASGPQLQFRDDTP
                     IHKDFHYKHVLVDGGLRVIDFDEVRLGDPAYDVAHFCVHLRLLACRALGDPAGYAGLE
                     RAFLAAYTRRAGGVPVDRYAWFATYTCLKIAKQLCTTRGVRPRPDGEELRRQVRIMLA
                     QGLAYSEDIP"
     gene            4826..>6044
                     /locus_tag="VF995_04220"
     CDS             4826..>6044
                     /locus_tag="VF995_04220"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9342806.1"
                     /translation="MTDLTGQRLGQYELHALIRRGGMATVYKAHQESLDRWVAVKVLE
                     RSGDPQFVARFRREARSVAQLLHPNIVPIFDYGEEDGRPFLVIQYVDGGQTLGDLMGE
                     ALEPALSIQLMLHLLEALGYAHERGIVHRDLKPGNVLMPTPIWPMLSDFGIVKLLLES
                     DQTQLTQQGLIVGTAAYMAPEQAFGMAVDARTDLYSAGVVLYELTCGQVPFEADTPVS
                     VLMKQAYEPPPSPRSRNPDLPPELERILLRALAKSPDDRYPTAADMAADLQQVLAELQ
                     PLPAVQRDEDPLDSLPGAYAQGVEAFAAGAWEEAIDHFTKVTTYDPGYEDAESLLATA
                     LAVRDQARPPHTAGPAAEPVDVPSAEPPVEPPVEPPVPAPAPVAEPPPAARPGPRPRP
                     SPSRWWLAVVALLL"
BASE COUNT          846 a         2134 c         2186 g          878 t
ORIGIN      
        1 cggtcgccgg gacggctgcc gggacggagg tgggggctgc ggcggcagtg gcggcgccgt
       61 gggcgatcgc ggcggtgcgg tcgatggtcg cggcggcgtc tccgccggcg gcagcggtgg
      121 tgcggtgggg ggtcgtggcg gtgcgtggtg ggtcatcgtc atccgttcga actcctgccg
      181 cgacgctggc agcggcagtg cctgcatcag cacggtctcg aacactttgc gccgcactcc
      241 cgccggcgcg gcgtcgtcgt ccagcgccgc cgcggtcagc gccgggaccg gcggtttcac
      301 cggcggcgac gccggcattg gcaggggccg gtcgtgctcg gccagggcac tttcggcgag
      361 gcctcgttcg acctcggcgg cgccgtactg gcggtggtag agatcggcat acaagccgcc
      421 ctgggcgagc agggactggt gggtgccctc ctggctgatg tggccgccct ccagcaccag
      481 gatccggtcg gcgtcgctga tcaggcccat gtcgtgggag atgaccaggg tcgtcttgcc
      541 acccgagagg agccgcagcg cctccagcac cgcgtcggtc gactcggcgt ccagcccggt
      601 ggtcggctcg tcgaggatca gcagtggggt gtcgcggatg aacgcgcggg cgatcgagat
      661 ccgctgccgc tggccgccgg acaggttctg ggcacgttcg gccagctcgg tgtcgtagcc
      721 gtcggggagc tgcaggatga agtcgtgagc gttggccttc ttggcggcct ccacgatctc
      781 ctcgcgggtg gcgccgtcgc ggccgtaggc gatgttgtcg gccaccgtgc cgttgaacag
      841 gatcgcctcc tgcaacacca tggtgacctg gtcgcgcagc gactccaggg tgaactcgcg
      901 cagatcgtgg ccgtcgatca gcacctgccc gtcatgcggg tcgtagaggc ggggggcgaa
      961 ctgggcgacg gtgctcttgc ccgcgccgct gtggccgatc agcgccaccg actcgcccgc
     1021 ccgcaccgag aagctcagat ggtcgagggc gagccgcaac cccgcgcccg cgcccacggc
     1081 gccgccgttg gcactggcct tggccttggc ggcgtctggc tcggcctggt aggcgaagct
     1141 gacctcgcgg aactccagat gtccgagcaa cggcggtgcc ggcaccgcgc cggggaggtc
     1201 ctgcaccgcc ggctcgcggt cgagcacctc gccgatgcgc tccacgctgg cgaacacctt
     1261 gccgagggtg ttccactcct tgatgatccg ccgggtcggc ttgaacatgt tctcgatcca
     1321 cagcacgaac gccaccagca ggccgagcga gagctgctgg cggtcaaaca ggaacagccc
     1381 catccagacg accccgctga tacacagtgc ctccagcacg ctcaccgtcc agctgaaggt
     1441 cgcctcgacc ctggcggcgt ccagggcggc attcatggcc tgctggttct ccttggcgaa
     1501 gcgctgcacc tcgcgaccgc cgcggccgta gatctggatc acccggatgg aggtgagcat
     1561 ctcctgcgcg gtggaggcga gctctccctc gcgtgcccgc tgccgtttgc tcgccgactt
     1621 gatccgcccg gtgaagaagt tcgagacgct ggccaggatc gggatgatca gcagggcgat
     1681 cagtgccacc tgcggcgact tgtagagcag gaaccccagc gtgccgacca ggatgaagac
     1741 gctgccgatg atgtctgagg acgactcgat gacgaagtcc tccagcgcgg tgacgtcgct
     1801 ggtcacccgg gcgagcacgt cgccggtgcg cccctggttg tggaacgcca gcgacagctt
     1861 ctgcaggtgg tcgtagagct tcacccgcag gttgaagccg agcatgcgac cgccgcgggc
     1921 gaagttgatc tcggccaccg agtcgcacag gctgttgacc gcggcgagca ccacgatcgc
     1981 gccggtcacc agcgccacgg tcatgttgcg ggagccgaac tgcccgctgt gggcgccgcc
     2041 cttgaggaag ttgatcaggt aggcgatcgg ccctggctca gccaccgcgg tgatcgactc
     2101 gatgaccagc atcagcagcg ccagccgcac gacccggcgc tggccggcga ggaaccggcg
     2161 gatcagccgg atcacccggc gggcgtcgcc gctctggccc ttggcgtcct tgcccttgcg
     2221 acgcttggcc ttgtcgttgg ccttcgcctg gtccttcatg agctacctcc ggcggagaca
     2281 gaagccgggg ccagcaacgc cgccagggtg gcggcggcat cgcggccgtc gaagcgctcc
     2341 accaccgtac ggcgggcctg gccgccgagg cgagcgcgca gccgctcgtc ggcgagcagg
     2401 acggcgaggt ggtcggcgat cgcgtccacg tcgcgcggtg gcgccagcaa cccatcggcg
     2461 ccgtggcgca tgacctcggt cacgccggcg acggtggtgc tcaccaccgg cagcccgcag
     2521 gccatcgcct ccagcagcac gttcgggatg ccgtcgcggt cgccgtcggc ggtgacgaac
     2581 ggtgtgaggg cgaacaggtc ggcgcgccgc aatgccgcac gcagctccgg ccgggtccgc
     2641 gacccggcga gcaccacctc gtcgccgagg ccgagccggt cgacggttgc cgccagctcc
     2701 tcgcccaacg gtccgtcgcc gtacagctcg aggtggaagc gctgcccgcg ctgcttgagg
     2761 cggtggcagg ccgccagcag gtcggcgaag cccttcttct cgaccaggcg gccgaccgac
     2821 acgatccgcg gcgccgcggc atcccgggcg gcgtcccggt ccccttcgcg gttgcggccg
     2881 ggaccatgac cggtggcggg ctggaatgcc tccacgtcga cgccgtggtg gatcaggcgt
     2941 acccgctcgc cgagcccgtc gccgagcaca tcgcgcaggt agtccgcgtt gggaccgcag
     3001 caggtgacca ccgcctcagc cgccgcgacc cggtcggcca cggccgctgg cggcacctgg
     3061 aagaggtcgc gggcgtgcgc ggtaaagctc cacgggatcc cggtcagccg gtgagtgagc
     3121 agggcgacca gcgcggggtc gtgcgcgaag tgggcatgca ggcgcccgac cgccccgcct
     3181 tccccctcgc ggccggcgcg ccgcaacgtc gccgccagcg tcgccgccag ggtcaggcag
     3241 cggccccgcg acgccgcgtg gtagccccgg tcccactcgg gatgccgcgc gacgtaccag
     3301 gccgtcgcca ggtagcgtgg tggcgacgcc agcaacgccc gcagatgcgc ggcaaccgcc
     3361 gtacggcgcc gcggggtgcc gccatcgagg tactcgactg gcgcgcgcac cgccgtcagt
     3421 tccggctggg tcagggcctc ggtggaggtg ctggtcaacg cgaagatccg cagccgcagg
     3481 ccgagccgct ccagtgccag cacctcgtcg agcacgaacg tctgcgacag gcgcggccag
     3541 ccgcgtacca ggacgcccag cgccggcgtt gccgggcgct ggggctcacg ggatgtcttc
     3601 actgtaggcg agtccctggg cgagcatgat ccgcacctgc cgccgcagct cctcgccgtc
     3661 ggggcgaggg cgcacgccgc gggtggtgca gagctgcttg gcgatcttca ggcaggtgta
     3721 ggtggcgaac caggcatagc ggtccaccgg cacgccgccg gcgcggcggg tgtaggcggc
     3781 gaggaaggcc cgctccagcc cggcgtagcc ggccgggtcg ccgagcgcgc ggcaggcgag
     3841 caagcgcagg tggacgcaga agtgcgccac gtcgtaggcg ggatcgccga ggcgcacctc
     3901 gtcgaagtcg atcacccgca ggcctccgtc caccagcacg tgcttgtagt ggaagtcctt
     3961 gtggatcggg gtgtcgtcgc gaaactgcag ctgcgggccg ctggcctgga ggcggtcgga
     4021 caggtgcgcg acggtgcttg ccaggtcggg atgggcctgg ctgatgagcg cgccccacgc
     4081 ttgcaggttg accagctcca gcgacggctg gaagcggcgg tccagctcga agcgggcgcg
     4141 gtgcagcgtc gcgagccagt cggcggtgcg ctcgatcgcg ccggcgcccc cggcgctgct
     4201 ctgcagcacc tcgtcgagga tctcgccctc caccgggatg tacaccagca tggccaggtc
     4261 ggggaggcag ccgagcgggc gcggcacacc gaggcggggc gtcgcggcga aggcggcggc
     4321 gaggccacgc agaatggtgt cgacccggcc ggcctggcca ggctgcgggt acagcttgcc
     4381 gagcacggtg gtcgggccac cttcggtggc gacctggtag cggatcgtgc cgcggttgcc
     4441 ctgcttgtag gcgagcaacc gggcgtaggt caccggcggc ggcccgtccg gccctgccag
     4501 cgcctccagc agcggcgcca gcttgtcggt catccgctgc acgttgagtg ccacctgcag
     4561 gatcggcagc ccggtcaggt gcagcgccgt gggatgcttg ccgacgctgg tggaggtcat
     4621 gacctcagcg atcccggtct cacgtggagg aggtgacccg ggcggcgttg gtcggctcgg
     4681 cacgcgcgtc aacccccctc ccctctcccc gctgagggtt ctgttggcga tactagcatc
     4741 ctgttcttat catccggttc gccaatttgt gcgaagtagt ttccatgtcg ttaacaatgg
     4801 cgtcggtgaa cgtttgggga ggcccgtgac ggacctgacc ggtcagcggc tcggccagta
     4861 cgagttgcat gcactcatcc gccgtggcgg catggcaacg gtctacaagg cgcaccagga
     4921 gtccttggac cgctgggtgg cggtgaaggt cctggaacgg tccggcgacc cgcagttcgt
     4981 cgcccgcttc cggcgggagg cgcgctcggt cgcccagctg ctgcatccca acatcgttcc
     5041 gatcttcgat tacggcgagg aggacggccg ccccttcctg gtgatccagt acgtggatgg
     5101 agggcagacg ctcggcgacc tgatggggga ggcgctggag ccggcgctct cgattcagct
     5161 gatgctccac ctgctggagg cgctcggcta cgcccacgag cgcggcatcg tccaccgcga
     5221 cctcaagccg ggcaacgtgc tgatgccgac gccgatctgg ccgatgctgt ccgacttcgg
     5281 catcgtcaag ctgctgctcg agagcgacca gacgcagctc acccagcagg gcctgatcgt
     5341 cggcaccgcc gcctacatgg cgccagagca ggcgttcggc atggcggttg acgcccgcac
     5401 cgacctctac tcggccggcg tggtgctcta cgagctgacc tgcggccagg tgccgttcga
     5461 agcggacacg ccggtgtcgg tgctgatgaa gcaggcctac gagccgccac catcgccgcg
     5521 cagccgcaac cccgacctcc cacctgagct ggagcgaatc ctgctgcgcg cgctcgccaa
     5581 gagccccgac gaccgctacc cgaccgccgc ggacatggcc gcggacctcc agcaggtcct
     5641 cgccgagctg caacccctgc cagccgtcca gcgggacgag gacccgctcg actccctgcc
     5701 gggcgcctac gcccaaggcg tcgaggcgtt cgccgcgggc gcctgggagg aggcgatcga
     5761 ccacttcacc aaggtgacca cgtacgaccc cggttatgag gacgccgagt cgctcctcgc
     5821 caccgccctc gccgtccgcg accaggccag gcctccacac accgcggggc cggcggccga
     5881 gccggtcgac gtgccctccg ccgagccgcc ggtggagccg ccggtggagc cgccggttcc
     5941 cgctcccgcg ccggtcgcgg aaccgccacc agccgcccgc cccggccccc gcccccgccc
     6001 cagccccagc cgctggtggt tggcggtggt cgcgctgctg ctcg
//
LOCUS       DASWDC010000132         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_281064, whole genome shotgun sequence.
ACCESSION   DASWDC010000132 DASWDC010000000
VERSION     DASWDC010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281064"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF995_04225"
     CDS             complement(<1..204)
                     /locus_tag="VF995_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9342807.1"
                     /translation="MSEILRTPTGTIYDRGYRHYDGPREGRSRGVRAIVVASIRRALG
                     LRRGWKTKVVPFGLLVLAFAPAAA"
     gene            complement(257..1240)
                     /locus_tag="VF995_04230"
     CDS             complement(257..1240)
                     /locus_tag="VF995_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008746915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9342808.1"
                     /translation="MPVAEVAGLSKRFGAVQALDHVDITVEAGCTGLVGSNGAGKSTL
                     IKLLLGLIAPDGGRATVLGHDVAADPLAIRAQVGYLPEGSCLPLDVTAADFVSFMAQL
                     GGLSPQPARERASEVCYQVGLYEERYRAIGGFSTGMQQRVKLAVALVHDPRLLLLDEP
                     TDGMDPQGREDMLELIDRVRSELRVDVLVSTHLLGDVERVCDRVVMVHAGHVLTQGSI
                     TAVAGTAEAGFAVQLGDNGEAVSAALTAAGLQVRPGADDLQVQGAAGAADVIRDLAAE
                     HGWPLRRLIARAPSLEETFLRLQATATATADGAAPVSAAAEPQRSPEGVPR"
     gene            1449..2591
                     /locus_tag="VF995_04235"
     CDS             1449..2591
                     /locus_tag="VF995_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEX9342809.1"
                     /translation="MTVVWDESLAAYDLGPEHPLRPARVRLTMALARSTGLLDQARLD
                     VPQPLSGNDLARVHDLDYLAAVKAASIDGHAIPDRGLGSGDTPSFPGVHDASALVCAA
                     TVTAAGVVIGGGPAHAFSPAGGLHHAMPGHASGFCVYNDPAVAIAWMLDQGVGRVAYV
                     DVDVHHGDGVEAIFAGDPRVLTVSVHESGRYLFPGTGEAHEIGAGAARGTIANLPLPP
                     TTTDDLYLAAFDALVPALVRAFEPEVLVTQLGCDSHYTDPLAHLGLTTRAYAQLAGRL
                     HDLAHTVAGGRWLATGGGGYQWATVVPRAWCTYLAEMVGVELPERLPGAYLAEVMEQH
                     GVALDPAMVDEVVALRSDRREWVERQIARSIEIARGNLFPLYGLGA"
     gene            2674..>3049
                     /locus_tag="VF995_04240"
     CDS             2674..>3049
                     /locus_tag="VF995_04240"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEX9342810.1"
                     /translation="MPEMTRDELGRHLVATRIAGQVATPVSSVLMKAERVAAGDPEHC
                     FGLSGMSRYSRDDVLAEITAQFGWTRSAGEPGDDPTYIDPELLLDELDRAGERLAGAT
                     RNGEHVLFATGHPTGVFSLHQQL"
BASE COUNT          409 a         1139 c         1079 g          422 t
ORIGIN      
        1 cgcggccgcc ggggcgaacg cgagcaccag cagcccgaac ggcaccacct tggtcttcca
       61 gccgcgacgc aggccgagcg cgcggcggat gctggcgacc acaatcgccc gcacccccct
      121 ggagcggccc tccctgggtc cgtcgtagtg ccggtagccc cggtcgtaga tggtcccggt
      181 gggtgtccgc agtatctcgc tcattcggca cgctccggca tcatcgtcca ccgtggcagc
      241 gcgctcaccg ttccgctcac cgtggcaccc cctcgggaga ccgctgcggt tcggcggcgg
      301 ccgagactgg ggccgcgccg tcggcggtgg cggtggcggt ggcctgcagc cgcaggaagg
      361 tctcctccag gctgggggct cgcgcgatga ggcgccgcag cggccagccg tgttcggcgg
      421 cgaggtcgcg gatcacgtcg gcggcaccgg cggcgccctg cacctgcagg tcgtcggcgc
      481 cggggcggac ctgcagcccg gcggcggtca gggcggcgct gaccgcttcg ccgttgtcgc
      541 cgagctggac cgcgaagccc gcctcggcgg tgccggcgac cgccgtgatc gagccctggg
      601 tgagcacgtg gccggcgtgg accatcacca cccggtcgca gacccgctcg acgtcgccga
      661 gcaggtgggt ggagaccagc acgtcgaccc gcagctcgct gcggacccgg tcgatcagct
      721 ccagcatgtc ctcgcggccc tgggggtcca tgccgtcggt gggctcgtcg agcagcagca
      781 ggcgcgggtc gtggaccagc gcgaccgcca gcttgacgcg ctgctgcatg ccggtggaga
      841 agccgccgat ggcccggtag cgctcttcgt agaggccgac ctggtagcag acctcgctgg
      901 cgcgctccct ggcgggctgg ggcgagaggc cgccgagctg ggccatgaac gagacgaagt
      961 cagcggcggt gacgtcgagg ggtaggcagg atccctccgg caggtagccg acctgggcgc
     1021 ggatcgccag cggatcggcg gcgacgtcgt ggccgagcac ggtcgcccgc cctccatcag
     1081 gagcgatcag tccgagcagg agcttgatca gggtggactt gccggcaccg ttggacccga
     1141 ccagtccggt gcagccggcc tcgacggtga tgtcgacgtg gtcgagcgcc tgcaccgccc
     1201 cgaagcgctt gctgaggccc gcgacctccg cgaccggcat gtcccacctc ccttggccga
     1261 gacccgaatc ccaacggcag ctccgctggc ctgagcaaca ctgcccgccc cgggcggccc
     1321 ggtcaaccca cctcaccaac ctgtggataa cgatccgggc gcgcattcga gggtacgctg
     1381 tgcccgagtt cgctcagaga tcacgaattc gtaaagctcg ggcaagggac ggtgcgatgc
     1441 ctggtccggt gaccgtcgtc tgggacgagt ccctggcggc ctacgacctc ggccccgagc
     1501 atccgctgcg gcccgcgcgg gtccggctga ccatggcgct ggcgcgctcc accgggctgc
     1561 tcgaccaggc acgcctcgac gtcccgcagc cgctctccgg caacgacctg gccagagtcc
     1621 acgacctcga ctacctggcg gcggtcaagg ccgccagcat cgatgggcac gcgattcccg
     1681 accgcggcct cggctccggg gacacgccgt cgtttcccgg cgtgcacgat gcctcggcgc
     1741 tggtctgcgc cgccaccgtg accgccgcgg gggtggtgat cggcggcggg ccggcgcacg
     1801 ccttctcgcc ggctggcggg ctgcaccacg ccatgcccgg tcacgccagc ggcttctgcg
     1861 tctacaacga cccggcggtg gcgatcgcct ggatgctcga ccagggggtc gggcgggtcg
     1921 cctatgttga tgtcgacgtc catcacggcg acggggtcga ggcgatcttc gccggcgacc
     1981 cgcgcgtcct gaccgtctcg gtgcacgagt cgggccgcta cctgttcccc gggactgggg
     2041 aggcgcacga gatcggcgcg ggcgcagcac gcggcaccat cgccaacctg ccgctgccgc
     2101 cgaccaccac cgatgacctc tacctcgccg ccttcgacgc gctggtgccc gcgctggtcc
     2161 gcgccttcga gccggaggtg ctggtcaccc agctcggctg cgacagccac tacaccgacc
     2221 ccctggccca cctcggcctc accacccggg cctacgccca gctcgccggc cgcctccacg
     2281 acctcgccca cacggtcgcg ggcggccgct ggctcgccac cggcgggggc ggctaccagt
     2341 gggccacggt ggtaccccgc gcctggtgca cctacctcgc cgagatggtc ggggtggagc
     2401 tgcccgagcg cctccccggc gcctacctcg ccgaggtcat ggagcagcac ggcgtcgccc
     2461 tcgacccggc catggtcgac gaggtggtgg cgctgcgctc cgaccgccgc gagtgggtcg
     2521 agcgccagat cgcccgctcg atcgagatcg cccgcggcaa ccttttcccc ttgtacggcc
     2581 tcggggcctg agcctccgta gaccccgggg tggacagggg tgctccgcta ccatgggaga
     2641 tggcttgaaa ccggactggt gctggagtgt cgtatgcccg aaatgacccg agacgaactt
     2701 ggccgccacc tggtggccac caggatcgcg gggcaggtgg ccacccccgt ttcgagcgtg
     2761 ctgatgaagg ccgaacgggt ggccgccggg gacccggagc actgcttcgg cctctccggg
     2821 atgagccgct actcgcgcga cgacgtgctc gccgagatca ccgcccagtt cggctggacc
     2881 cgcagcgccg gcgagccggg cgacgacccc acctacatcg atccggagct gctgctcgac
     2941 gagctggacc gcgcgggcga gcggctcgcc ggcgcgaccc gcaacggcga gcacgtgctg
     3001 ttcgccaccg ggcacccgac cggcgtgttc agcctccacc agcagctct
//
LOCUS       DASWDC010000133         9331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_281230, whole genome shotgun sequence.
ACCESSION   DASWDC010000133 DASWDC010000000
VERSION     DASWDC010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281230"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VF995_04245"
     CDS             <1..642
                     /locus_tag="VF995_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9342811.1"
                     /translation="PEEVAAIGRWAAERGLWVITDEIYEHLVYGDNQFSSMPALVPEL
                     AERCVVVNGVAKSYAMTGWRVGWLIGPPDVVRAAINLQSHLTTNVANVSQAAALAAVS
                     GDLEAVAEMRVAFDRRRRRVHELLNAIPGVVCVEPQGAFYAFPSVKGVLGREVAGHRP
                     QTSLELAEVILEEAKVAIVPGEAFGAPGYARLSYALGDDDLVEGVTRIAKLLG"
     gene            complement(958..1557)
                     /locus_tag="VF995_04250"
                     /pseudo
     CDS             complement(958..1557)
                     /locus_tag="VF995_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636279.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
     gene            complement(1585..2709)
                     /locus_tag="VF995_04255"
     CDS             complement(1585..2709)
                     /locus_tag="VF995_04255"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX9342812.1"
                     /translation="MTMPKRRIRLALLYGGRSAEHPVSVVSARSVTEALDPDRFEVVP
                     IGITRDGAWMLPAKPPLELTAPEGGLPEVEAAGTAVALRPERHAAALAPLGAGGAQAP
                     DLALDAVDVVFPLLHGPYGEDGTVQGLFELADLPYVGSGVLASALAMDKAMAKVVLAA
                     AGIEQAPYLVVRAGEWRADPDGVYARVGGQLAYPVFTKPARLGSSIGITKVKAPEALA
                     DGLEAAFAHDAKVVVEQGLTARELECGVLGNEAPEASVVGEIVPGHEFYDFEAKYLDD
                     AAKLVIPAPVPDAVAERVRTLSLRVFQALDCAGLARVDFFYEEASGRVVVNEVNTIPG
                     FTPRSMFPMLWAASGVSYSDLVAKLVDLAVQRHAASADKR"
     gene            complement(2706..3881)
                     /locus_tag="VF995_04260"
     CDS             complement(2706..3881)
                     /locus_tag="VF995_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9342813.1"
                     /translation="MGRRFGTRAVHGADLPPPAQQPLAIPIWQTSAFAFDDADQFAHT
                     LAQPREGYVYSRYENPTTAALEATVADLEGAAEGLAAASGMAAIATVLLSLAGAGDHL
                     VVQRDVYGGTFSLATELAGRFGIETTVVDVADPAAVAAALRPTTRALYAETIANPTMA
                     VADLPALAEATRDAGVPLVVDNTVASPYLCRPIEHGAAIVVHSATKYLGGHSDVIGGL
                     ALFADRAAHAAAWRTMLDLGGSADPFAAWLVLRGIKTLPLRMERHGANAARVAALLEG
                     HPKVERVYWPGMPDHPSHDTAKRLLDGYGGFLSFDLAGGREAGRRFIEATRVARLAPS
                     LGGPETLVAHPASTTHRQLAAAALAAAGIGAGMVRVSVGLEDADDLCDDFSQALEVA"
     gene            complement(3896..4447)
                     /locus_tag="VF995_04265"
     CDS             complement(3896..4447)
                     /locus_tag="VF995_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342814.1"
                     /translation="MTMPMPEPEMKAAEAAAPAVERFQPGGVAQALVGGLLELALAAI
                     LLAASLEAGRRSQSAAATSLAVAAALWAALSLFSLATSALRAKVLAAELDDEAVVLHG
                     LGGARRYRYDELSAVEVSRARTRLVARDGRTRPVRGVRGASQGRRFRTRVLARATEAA
                     GRHAASLSGGAEQAPEPEPEGPS"
     gene            complement(4496..5167)
                     /locus_tag="VF995_04270"
     CDS             complement(4496..5167)
                     /locus_tag="VF995_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9342815.1"
                     /translation="MPVYALAKTVVPPVIRFWVRLDCQGLDNVPHEGPVIIAANHVSY
                     FDPLCLGVYIHTAGRQVRFLAKAELFRNPVLRWVLLGAGQIPVYRESRDAARSLADAV
                     DAMAEGAAVAIYPEGTTTRNPDFSPMPAKSGVARLAALTGAPVVPVGQWGAQVLFTRG
                     KIGPFRRGARVVLRAGSPLDLGLTPDSTLDALNAGRDKVMAAIVALVDEAKQDWTPPR
                     WYRGQ"
     gene            5209..5904
                     /gene="cofC"
                     /locus_tag="VF995_04275"
     CDS             5209..5904
                     /gene="cofC"
                     /locus_tag="VF995_04275"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX9342816.1"
                     /translation="MLLPLLPVKSLATSKGRLAPPLNPIERRLLTIAMLEDVIAALQA
                     AAMELEGGRLGRPVVISPDREVWRRAEALGCRVVEEPPPTDADGDGDGDGLNRSLARA
                     ANTGVVKDAGLLVVAADLPLASAAAFTRVGEAAERAPVVVVPSYDGTGTNVLAWRDAA
                     SFAPSFGRGSAARHLALPGAVRLDEPSLAHDVDTVADLRAVAISLDPASVTAQRLQAV
                     RSLANIAANVAEE"
     gene            5907..6137
                     /locus_tag="VF995_04280"
     CDS             5907..6137
                     /locus_tag="VF995_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342817.1"
                     /translation="MPDALLQGTVKSFDQASKAAVVLTDTRVEYPVPPEAFRRSGLRM
                     LQLGQRVKFRVEGEGGDARVTYVNILTLPDPE"
     gene            complement(6240..6770)
                     /locus_tag="VF995_04285"
     CDS             complement(6240..6770)
                     /locus_tag="VF995_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9342818.1"
                     /translation="MNRKELVDAIQQHTEVSRADVERVLSSLLQQTQLAVKKGERVSL
                     VGFGTFERRDRGARTARNPRTGDVVKVKATKVPAFRPGQGFKDAVSGKAPAAKGALAA
                     RPVRSAGASKPAAKMAAAPVAPSGAGLDGKAKGKGKAKADGKTSAGGGKATKAAKASK
                     TSKASAKVAAKSGKKK"
     gene            7517..8539
                     /locus_tag="VF995_04290"
     CDS             7517..8539
                     /locus_tag="VF995_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9342819.1"
                     /translation="MTGQARLADIAVHAGVSEATVSRVLNDKAGVAPTTRQAVLAALD
                     LLGYERPAKLRTRSAGLIGLVVPELDNPVFPVFAQEIESMLAQHRYTPVLCTQTPGGV
                     TEDDYVEMLLERGVSGIVFVSGLHADSTSDPARYLRLVEKKLPIVLVNGYMARIDAPF
                     ISTDDEEAAEMAVNHLVALGHERIGLAVGQERFVPCLRKCNGFRRAMKALLDQDDVTG
                     LIAHSLFSIEGGRVAATRLLSKGVTAIICSSDVMALGAIRAVRQGGLKVPDDVSVIGY
                     DDSLFVAFIDPPLTTVRQPVQAMARTAVQFLLDELAGIPAPRDEYVFRPELVVRGSTN
                     RCRAAP"
     gene            8763..>9331
                     /locus_tag="VF995_04295"
     CDS             8763..>9331
                     /locus_tag="VF995_04295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9342820.1"
                     /translation="MQAIIGQPSHANDASAPLRIAVVAPPWFEVPPEGYGGIEAMCAT
                     LVNKLVSRGHEVFLLGAGRDRTRGRFVPVLAEPPSIRLGDPMVETSYASRSARALREL
                     DVDVVHDHCLAGPLSAAGRFAPTVVTVHGPVHADLGDYYRALDDTIGLVAISEAQRRH
                     APDLPWEGTVHNGVVVDDFPFRDRAAKED"
BASE COUNT         1305 a         3322 c         3362 g         1342 t
ORIGIN      
        1 cccgaggagg tcgccgcgat cggccgctgg gccgccgagc ggggcctgtg ggtgatcacc
       61 gacgagatct acgagcacct ggtctacggc gacaaccagt tctcctccat gccagcgctg
      121 gtgcccgagc tggccgagcg ctgcgtcgtg gtcaacggcg tcgccaagag ctacgcgatg
      181 accggctggc gggtgggctg gctgatcggc ccgccggacg tggtcagggc ggccatcaac
      241 ctgcagtcgc acctgaccac caacgtcgcc aacgtgagcc aggcggcggc gctggcggcg
      301 gtctccggcg acctggaggc cgtggccgag atgcgggtcg ccttcgaccg gcggcggcgc
      361 cgcgtccacg agctgctgaa cgcgatcccc ggggtggtct gcgtggagcc gcagggcgcc
      421 ttctacgcgt tcccgtcggt caaaggggtg ctcggccgcg aggtagccgg acaccggccg
      481 cagaccagcc tggagctggc cgaggtgatc cttgaggagg ccaaggtcgc gatcgtgccc
      541 ggcgaggcgt tcggcgcgcc cggctacgcg cgcctgtcgt acgcactcgg cgacgacgac
      601 ctggtggagg gcgtcacccg gatcgccaag ctactcggct gagccctcgg ccgggcctgc
      661 ggcgtagtcg agcggcacgg tggtggtcag cttgtcgccg ctgtcggtgg tgacggtcag
      721 ggtgaggctg cgcacccccg gcggcggcgt cggggagaag ctcacctggt agaggtgcag
      781 gtgcttgtgg ccgccgacag agatgctgtg gcagatgtac ttcgtgccgg cgtcgtcggc
      841 agccacccag tcgccgaggc cacggatgga ctgtggcggg atcatcttgg cgccggtcgc
      901 cggcagcgcc gcgaagcgca ggtcgaacca gccggaccag acctccagcg cgagcagcac
      961 cagccgtacg cccgcttcgg cctccagttg gcggccgatg gccacgacgc cgacgaactt
     1021 gggcgcccgg ctgcgcggcg ccatctggaa gcccacaccg atcgccgccg caggcggtgt
     1081 tgtgatctgg gccgcatctg cgggtaccgc ctcgccaggc tcgccggaga ggagctggag
     1141 gaccgcctgc cgcgcccgcg cgcgatcggc gccgagcttg gcgagcactt gggcgccaac
     1201 gccctcgccc tcgcggacga ggccgaggag caggtgctcg gtgccgatgt agttgtggcc
     1261 gagttgcagc gcctcacgca gcgacagttc caggatcttc ttggcgcgtg gcgtgaatgg
     1321 gatgtgcatg ctctgcggct tcttcttgcc gggtttgacc gccttctcga tttcgctgcg
     1381 gaccgtctcg agggacacgc cgagcacttc gagcgcccgg gcggctacgc cctcttgctc
     1441 ctggatcagc ccgagcaaca ggtgctcggt gccgatccag tcgtggtcga ggaggcgggc
     1501 ctcctcctgg gcgagtacga ccacccggcg ggcacggtca gtgaagcgtt cgaacatggg
     1561 ggcgggcccc tttgcgacgg ggtgtcagcg cttgtcggcg ctggcggcgt ggcgctggac
     1621 ggcgagatcg accagttttg cgacaagatc agaataactc acgccggagg ccgcccacag
     1681 catcgggaac atgctccgcg gcgtgaaccc ggggatggtg ttgacctcgt tgaccaccac
     1741 gcggccgctg gcctcctcgt agaagaaatc gacccgggcc aggccggcgc agtcgagcgc
     1801 ctggaagacc cgcagcgaca gcgtccgcac ccgctcggcg acggcgtcgg gcaccggcgc
     1861 ggggatcacg agcttggcgg cgtcgtcgag gtatttcgcc tcgaagtcgt agaactcgtg
     1921 accagggacg atctccccca ccacgctggc ttcgggggcc tcgttgccga gcacgccgca
     1981 ttccagctcg cgcgcggtga gaccctgctc caccaccacc ttggcgtcgt gagcgaaggc
     2041 ggcctccagg ccgtcggcga gcgcctcggg cgccttcacc ttggtgatgc cgattgagga
     2101 gccaagccgc gccggcttgg tgaacaccgg gtacgcgagc tggccgccga cacgcgcgta
     2161 gaccccgtcg ggatcggcac gccactcgcc cgcacggacc accaggtagg gggcctgctc
     2221 gatgccggcg gcggcgagca ccaccttggc catggccttg tccatcgcca gcgccgaagc
     2281 gagcacgccg ctgccgacat agggcagatc ggccagctcg aacagcccct gcacggtgcc
     2341 gtcctcgcca taggggccgt gcagcagcgg gaagaccacg tcgacggcgt ccagcgccag
     2401 gtccggggcc tgcgcgccgc cggcgccgag tggggcgagg gcggcagcgt gacgctccgg
     2461 ccgcagcgcc acggcggtgc cggcggcctc gacctcgggc aggccgccct ccggcgcggt
     2521 cagctccagc ggcggcttgg ccggcagcat ccaggcaccg tcgcgggtga tgccgatcgg
     2581 gaccacctcg aaccggtcgg ggtccagcgc ctcggtcacc gaccgggccg acaccaccga
     2641 gaccgggtgc tcggccgagc gccctccgta cagcaacgcc aggcggatcc gccgcttcgg
     2701 catcgtcatg ccacctccag ggcctgagag aagtcgtcgc agaggtcatc ggcgtcttcg
     2761 aggccaaccg aaacccgcac catgcccgcg ccgatcccgg cggcggccag cgcggcggcg
     2821 gcgagctgcc ggtgggtggt cgaggcgggg tgcgccacca gcgtctctgg accgcccagc
     2881 gacggcgcca gccgggccac gcgggtcgcc tcgatgaagc ggcggcccgc ctcgcgcccg
     2941 ccggccaggt caaaggagag gaagccgccg tagccgtcca gcagccgctt ggcggtgtcg
     3001 tggctgggat ggtccggcat ccctggccag tacacccgct cgaccttggg gtggccctcc
     3061 agcagcgcgg cgacgcgggc ggcgttggcg ccgtggcgct ccatgcgcag cggcagcgtc
     3121 ttgatgccgc gcagcaccag ccaggcggcg aacgggtccg cggagccgcc gaggtcgagc
     3181 atggtgcgcc acgccgcggc gtgggcggcc cggtcggcga acagcgccag cccgccgatc
     3241 acgtcgctgt ggccaccgag gtacttggtg gcggagtgga ccacgatggc ggcgccatgc
     3301 tcgatcgggc ggcacaggta gggcgaggcg acggtgttgt cgaccaccag cggcaccccg
     3361 gcgtcgcggg tggcctctgc cagggcgggc aggtccgcca cggccatggt cgggttggcg
     3421 atcgtctcgg cgtacagcgc ccgggtggtg ggccgcagcg ccgcggcgac cgcggcgggg
     3481 tcggcgacgt cgaccacggt cgtctcgatg ccgaagcggc ccgccagctc ggtcgccagc
     3541 gagaacgtcc cgccgtacac gtcgcgctgc accaccaggt ggtcgccggc accggcgagg
     3601 ctcaacaaca ctgtcgcgat cgccgccatg ccggaggcgg ccgccaggcc ctcggcggcg
     3661 ccctccaagt cggccacggt ggcctccagc gccgcggtgg ttgggttctc gtagcggctg
     3721 tagacgtagc cctcccgcgg ctgcgcgagg gtgtgggcga actggtcggc gtcgtcgaag
     3781 gcgaacgctg acgtctgcca gatcgggatg gcgagcggct gctgcgccgg cggcggcagg
     3841 tcggctccgt ggactgctcg ggtgccaaag cgtcggccca ttcgttccct cccggtcagc
     3901 tcggtccctc cggttctggt tccggtgcct gttcggcgcc gccgctgagg ctagcagcgt
     3961 ggcgaccggc cgcctcggtg gcgcgggcca ggacccgcgt gcggaagcgc cggccctggc
     4021 tggcgccgcg gacgccgcgg acggggcgcg tccgcccgtc gcgcgccacc agccgggtgc
     4081 gggcgcggga cacctccacc gccgacagct cgtcgtagcg gtagcggcgc gcgccgccga
     4141 gcccatgcag caccaccgcc tcgtcgtcca gttcggcggc gagcaccttg gcccgcagcg
     4201 ccgaggtcgc caggctgaag agcgacagcg ccgcccacag cgccgccgcg accgccagcg
     4261 aagtggcggc ggccgactgg ctgcggcggc cggcctcaag ggaggcggcc agcaggatcg
     4321 cggccagcgc aagttccagc aggccgccga ccagcgcctg cgccaccccg ccgggctgga
     4381 accgctcgac cgcgggcgcc gcggcctccg ccgccttcat ctcgggctcc ggcattggca
     4441 tggtcattgg cgcatcgctc gcgtcgttgg ccgcatcgcc gcgctcacct ggcggtcact
     4501 ggccgcggta ccaccggggc ggcgtccagt cctgcttggc ctcgtcgacc agcgcgacga
     4561 tcgcggccat gaccttgtct cggccggcgt tgagggcgtc cagggtcgag tccggggtga
     4621 ggccgaggtc gaggggcgac ccggcgcgca gcaccacccg ggcgccgcgc cggaacgggc
     4681 cgatcttgcc gcgggtgaac agcacctgcg cgccccactg gcccacgggg accaccggcg
     4741 cgccagtgag cgcggcgagc cgggccacgc cgctcttggc tggcatcggg gagaagtccg
     4801 ggttgcgggt ggtggtgccc tccgggtaga tcgccacggc ggcgccctcg gccatggcgt
     4861 cgacggcgtc ggcgagcgac ctggcggcgt cgcggctctc ccggtacacc gggatctgcc
     4921 cggcgccgag caggacccag cggagcaccg ggttgcggaa cagctcagct ttggccagga
     4981 accgcacctg ccggccggcc gtgtggatgt agaccccgag gcacagcggg tcgaagtagg
     5041 agacgtggtt ggctgcgatg atcaccggcc cctcgtgggg aacgttgtcc agcccctggc
     5101 agtcgagccg tacccagaac cggataaccg gcggcacgac ggtcttggcc agggcataga
     5161 cgggcattcc tcggcgcgcc atgctgccgc gatagtacgc tgcacgccgt gctgctaccg
     5221 ctgctgcccg tcaagtccct cgccacgtcg aagggccgcc tcgcgccgcc gctgaacccg
     5281 atcgagcggc gcctgctcac catcgccatg ctggaggatg tgatcgcggc gttgcaggcc
     5341 gcggccatgg agctggaggg cggccggctc ggccgtccgg tggtgatctc gcccgaccgg
     5401 gaggtgtggc gccgcgccga ggcgctcggc tgccgagtgg tcgaggagcc gccgcccacc
     5461 gacgctgacg gcgacggtga cggtgacggg ctcaaccggt cgcttgcccg cgccgccaac
     5521 acgggcgtcg tgaaggacgc cgggctgctg gtggtcgcgg ccgacctgcc actcgcatca
     5581 gcggcggcgt tcacgcgggt gggcgaagcc gcggagcggg caccggtggt ggtcgtgccg
     5641 agctatgacg gcaccggcac caacgtgctc gcctggcgcg acgccgcctc attcgcgccg
     5701 tcgttcgggc gcggcagcgc cgccagacat cttgccctgc ccggcgcggt gcggctcgac
     5761 gagccgtcgc tcgctcacga cgtcgacacc gtcgccgacc tccgcgccgt cgccatcagc
     5821 ctggatccag ccagcgtgac cgcgcagcgc ctgcaagccg tgcgcagcct ggccaacatc
     5881 gccgccaatg tcgccgagga gtgaccatgc cggacgcttt gctgcaaggg accgtgaaga
     5941 gctttgacca ggccagcaag gcggccgtgg tgctgaccga tacccgcgtg gaatacccgg
     6001 tgccgcccga ggcgttccgg cgctccggcc tccgcatgtt gcagctcggc cagcgggtca
     6061 agttccgggt cgaaggcgaa ggcggcgacg cgcgcgtcac ctacgtcaac atcctgacgc
     6121 tccctgatcc cgaataacgc gaaaacgtcg taaccgacac ggaaaacgcg agcggcccct
     6181 gcgcggggca ggggccgcct tctggtttca ggcttccgga tttcgaagcc ggcacagcgc
     6241 tacttcttct tgccggactt cgccgccacc ttggcgctcg ccttcgaggt cttcgacgcc
     6301 ttggcggcct tggtagcctt gccgccgccc gcgctcgtct ttccgtccgc cttggccttg
     6361 cctttgcctt tggccttgcc gtcgagcccg gcgccgctgg gcgcaaccgg tgccgcggcc
     6421 atcttggcag cgggcttgga ggcgcccgcc gaccgtaccg gcctggccgc cagcgccccc
     6481 ttggcggcgg gggccttgcc gctcaccgcg tccttgaagc cctggcctgg acggaacgcc
     6541 gggaccttgg tcgccttgac cttgaccacg tcgccggtgc gggggttccg cgcggtacgg
     6601 gcaccgcggt cgcggcgctc gaaggtcccg aagcccacca gtgacacccg ctcgcccttc
     6661 ttgaccgcga gctgggtctg ctgaagaagg ctgctgagca ctcgctcgac atccgcccga
     6721 gacacctcgg tgtgctgctg aatggcgtcg acgagttctt tgcggttcaa ggcgcctcct
     6781 tgcccaatag ggaacattcc ccgcccggga cacggccacc gaaattagcc aagccctgtc
     6841 cctttcggag ctgcatgcta aagagccaca taagaaaggt caacaacccc gagcgctgaa
     6901 ccccttgagc gacaaggcgc tccgccccgg aggccgcgtt cgcggccacc tgaattgcga
     6961 acggcctcaa gcccggacga tggaatatgc gcaggtttgt gctggctcgc aaccggcttc
     7021 tcgtttcccg actcacattt cctttcgggc ggtccattac cctgatcaac gatctggata
     7081 ctcccactcc ctatcggctt cagtatcggt cgctggagcg actgctggag cgaggatgcg
     7141 gggaaacaac ggcgcggggt ccggtaggcg cctgtcgcgg cggcggcggg caggaacggt
     7201 gcaagttccc cagcgagggt ccggcaggcg tccaccagca gcgccagcgg ttcccggagc
     7261 gccgcgaacg cggcggcgcg gcggtcgacg agctggcgca cgggcacgcg ctgcgcctcg
     7321 gcggcgcggg tggtcttgac ggtgtgggcg accatgatcg gtgcgctcgc gttgtcctcg
     7381 gcggcggcct gtgccagggc gcgctccagc ggctcggccg tcgtagccgc atgtgagggc
     7441 gaccccggcg ccagggtcgc gccgccggat cgcggcgagg acctcgccgg cgtcgtcgcc
     7501 gtggtggggt gcccgcatga ccggacaagc gcgactcgcc gatatcgccg tccacgccgg
     7561 cgtcagcgag gcaactgtga gccgggtgct gaatgacaag gccggggtcg caccgaccac
     7621 ccggcaggcg gtgctcgccg cgctggacct gctgggctac gagcggcccg ccaagctgcg
     7681 gacccggtcg gcgggcctga tcgggctggt cgtccccgag ttggacaacc cggtcttccc
     7741 ggtcttcgcg caggagatcg agtcgatgct cgcacagcac cgctacaccc cggtgctgtg
     7801 cacccagacc ccaggcggcg tcaccgagga cgactacgtg gagatgctgc tggagcgcgg
     7861 cgtctccggg atcgtgttcg tgtccggcct gcacgccgac tccacctccg accccgcacg
     7921 ctacctccgg ctggtggaga agaagctgcc aatcgtgctg gtcaacggct acatggccag
     7981 gatcgacgcg cccttcatct ccaccgacga cgaagaggcg gccgaaatgg ccgtgaacca
     8041 cctggtcgcg ctggggcacg agcgcatcgg gctggcggtg ggccaggaac gcttcgttcc
     8101 gtgcctgcgc aagtgcaacg gcttccgcag ggcgatgaag gcgctgctcg accaggacga
     8161 cgtcactggt ctgatcgccc attcgctgtt ctccatcgaa ggcggccggg tcgccgccac
     8221 gcgcctgctc agcaagggcg tgacggcgat catctgcagc tccgacgtga tggcgctcgg
     8281 ggcgatccgc gcggtgcgcc agggtggact gaaggtcccc gacgacgtct cggtgatcgg
     8341 ctacgacgac tccctgttcg tcgccttcat cgacccgccc ctgacgacgg tgcgccagcc
     8401 ggtgcaggcg atggcgcgca ccgccgtgca gttcctgctc gacgagctgg ccggcatccc
     8461 cgcgccacgc gacgagtacg tcttccggcc ggaactggtg gtgcgcgggt cgaccaaccg
     8521 ctgccgggca gcaccctgag ccatcggtgc ccaggcgcac cggcggcgca tgctgcatct
     8581 tgcgtgttgc accctggcgg gcgtgacaaa agccggacgg cgggtcctcc cccggtaccc
     8641 aaacccccac gcagcgtggt ctgaacgcgt cgctccgtcg aaattccggc gaagccctcg
     8701 acaagatccc ggcttcggta taaaaacgcg ttttgccgtt cccgggacga tcgggggaat
     8761 caatgcaggc aatcatcggt cagccgagcc atgccaatga cgcgtccgca ccgctccgca
     8821 tcgccgtggt ggccccgccc tggttcgagg tgccgccgga gggctacggc ggcatcgagg
     8881 cgatgtgcgc cacgctggtc aacaagctgg tcagccgcgg gcacgaggtg ttcctcctcg
     8941 gcgccggacg cgaccggacg cgcgggaggt tcgtgccggt gctcgctgag ccgcccagca
     9001 tcaggctcgg cgacccgatg gtggagacca gctacgcctc ccgcagcgcg cgggcgctgc
     9061 gggaactcga tgtcgacgtc gtccacgacc actgcctcgc cgggccgctg tcggcggccg
     9121 ggcggttcgc gcccaccgtg gtgaccgtcc acgggccggt ccacgccgat ctcggcgact
     9181 actaccgggc actggacgac accatcggct tggtcgccat ctccgaggcg caacgccgcc
     9241 atgcccccga cctgccctgg gaggggacgg tgcacaacgg cgtggtggtg gacgacttcc
     9301 ccttccggga ccgtgccgcc aaggaggact g
//
LOCUS       DASWDC010000134         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1283622, whole genome shotgun sequence.
ACCESSION   DASWDC010000134 DASWDC010000000
VERSION     DASWDC010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283622"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VF995_04300"
     CDS             <1..207
                     /locus_tag="VF995_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342821.1"
                     /translation="QRLSESMTVLELARNGDIEPPIDLSIQGDGQGGLIWALRNGDNY
                     HLKGSDVTLADVIARVDKPYSPGG"
     gene            262..549
                     /locus_tag="VF995_04305"
     CDS             262..549
                     /locus_tag="VF995_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphorylase"
                     /protein_id="HEX9342822.1"
                     /translation="MATRVLATDQARSTIAQMNSVLSGGLQDQITQLDNLGKTLSDPN
                     VWDGPTAVDFRSNVWPGVNTSLQNTLSSLEELRAKLDTVTKNIMAAGGNAS"
     gene            723..>1946
                     /locus_tag="VF995_04310"
     CDS             723..>1946
                     /locus_tag="VF995_04310"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9342823.1"
                     /translation="MARKRGTSPSNAVDSEVPGYEELEAVGRGGFATVYRARQERFDR
                     TVALKVLAVDLVDERARRRFRRECLLAGRLSEHPNVVTVYDAGTTRAGRPFIAMEYCE
                     AGSLADRLRQHGPLPLDEVLRTGVKLAGALAAAHEAGIIHRDVKQQNVLLSRFGEPAL
                     ADFGVAAFANAAENSVVTDALTPVHAAPEVLEGDPATAASDLYSLGSMLYALLAGGAP
                     YARQAGEGIVPLLLRILEGEAPRIPRDDVPDRVMAFLRQAMSKDPAQRFGGDATAFGQ
                     RLQQLQAGLGLAVADLVRAAPAMAGPPLPDAGPVSPTDTTPRQRPGSSTVERRRPRPP
                     GAGAAPAPLPGAPAPPVTPAAAPVSPLAPPAPVAAHAQAPAPAAAPVRGPDPAPVPAP
                     ASGLGNGFGPGPDLEP"
BASE COUNT          282 a          744 c          685 g          235 t
ORIGIN      
        1 cagcgcctgt ccgagagcat gaccgtgctc gagctcgcca ggaacggcga catcgagccg
       61 ccgatcgacc tgagcatcca gggcgacggc cagggcggcc tgatctgggc gctccggaac
      121 ggggacaact accacctgaa aggctcggac gtgaccctcg cggacgtgat cgcgagggtg
      181 gacaagccct actcgcctgg tggatgaccc agctacccga atccctcgac cctatcccca
      241 cctgccaagg aggcagcagc aatggcgacc agagtcctcg caaccgacca ggcccgcagc
      301 accatcgcac agatgaactc ggtgctcagc ggcggcctgc aagaccagat cacccagctc
      361 gacaacctcg gcaagaccct cagcgacccc aacgtctggg acggccccac cgcggtggac
      421 ttccgctcca acgtctggcc gggggtcaac acctcgctgc agaacaccct gtcgtcgctg
      481 gaggagctgc gcgccaagct cgacaccgtg accaagaaca tcatggccgc cggcggcaac
      541 gcgtcgtaac cctgcccctg tcaccagggt cgtggggcct cgccaatccg ccacctcacc
      601 ggcggggccc cacgatcatc ggcattcctg gcatcaaggc gagccaggac gcctgaccag
      661 atccctcggg agcagggctt gacccccgtc acgaggcgga gcaaggagta gttaggagcc
      721 gtatggcgcg caagcgcggg acctcgccgt cgaacgccgt tgacagcgag gtccctggct
      781 acgaggagct ggaggcggtc gggcggggcg ggttcgccac cgtgtaccgc gcccggcagg
      841 agcggttcga ccgcacggtg gcgctcaagg tgctcgctgt cgacctggtc gacgagcggg
      901 cgcggcggcg cttccggcgg gagtgcctgc tggccgggcg gctgagcgag catcccaacg
      961 tggtgacggt ctacgacgcc gggaccacca gggcggggcg gccgttcatc gccatggagt
     1021 actgcgaggc gggctcgctg gccgaccggc tacgccagca cggcccgcta ccgctcgacg
     1081 aggtgctgcg gaccggcgtg aagctggccg gggcgctggc cgccgcgcac gaggccggga
     1141 tcatccaccg cgacgtcaag cagcagaacg tgctgctgtc gcgcttcggc gagccggcgc
     1201 tggccgactt cggcgtggcc gcgttcgcca acgcggcgga gaactcggtg gtcaccgacg
     1261 cgctgacccc cgtccacgcc gcgccggagg tgctggaggg cgacccggcg accgcggcgt
     1321 cggacctgta ctcgctgggc tcgatgctgt acgcgctgct ggcgggcggg gcgccctacg
     1381 cgcggcaggc cggcgagggg atcgtgccgc tgctgctgcg catcctggag ggcgaggcgc
     1441 cgcggatccc ccgcgacgac gtgcccgacc gggtgatggc cttcctgcgg caggccatgt
     1501 ccaaggaccc ggcgcagcgc ttcggcggcg acgccaccgc cttcgggcag cgcctccagc
     1561 agctccaggc cggcctcggc ctggcggtcg ccgacctggt ccgcgcggcg ccggccatgg
     1621 ccgggccccc gctgcctgac gccggtccgg tcagcccgac ggacacgacc ccccgccagc
     1681 ggcccggctc ctccacggtc gagcgccgcc gcccgcggcc gcccggcgcc ggggccgcgc
     1741 ccgcgccact tcccggcgct cccgctccac ccgtgacgcc agcggcggcg ccggtctcgc
     1801 cgctggcgcc gccggcacca gtcgcggcgc acgcccaagc tccggctcca gccgcggctc
     1861 cggttcgcgg gcccgatccg gccccggttc cggcacccgc cagcggcctc ggcaacggct
     1921 tcggccctgg tcccgacctg gagccg
//
LOCUS       DASWDC010000135         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_350623, whole genome shotgun sequence.
ACCESSION   DASWDC010000135 DASWDC010000000
VERSION     DASWDC010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350623"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VF995_04315"
     CDS             complement(<1..151)
                     /locus_tag="VF995_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9342824.1"
                     /translation="MRGGGTAAAVADVVIVGGGPVGLWLAAELHLAGVRVVVLEQLAE
                     RLPYSK"
     gene            complement(198..1148)
                     /locus_tag="VF995_04320"
     CDS             complement(198..1148)
                     /locus_tag="VF995_04320"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9342825.1"
                     /translation="MREIIIGHVAPAYVGVALWVAEHEGILERHGVGVTSVILGTTEG
                     TTAALRDRTVDVAMTAPEGTIADAVAGGPLRVVAGIANKPPLSLIALPRHHAIADLRG
                     GRIGTSSLKEGTKHLAEAMLAAEGLTWPRDYDFALEGNHQARWRALQDGSIDAALQLV
                     PYNYLAQEAGFSNLGDADASVPEFAFSAVCAHRDYLAANGEAVSALLAALLEATRWFY
                     GDVEGAAAIAAARTQLPRRHALRACQELAGKQVLPPDLRVTPGALAAAVGALRASGQL
                     PPGASTATAEPESGSAWVGEAALDYTCLDYTCLDRAAASG"
     gene            1363..>1916
                     /locus_tag="VF995_04325"
     CDS             1363..>1916
                     /locus_tag="VF995_04325"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017627280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9342826.1"
                     /translation="MHPAGRTGLQFYDLSHTFGPNAPLWPYFEDVKIERLHYHAKSGV
                     LSQRISTVMHCTTHLDAPAHVVEGTPYTDEVPLEQFFGTGVAVSIPKKQWEVITPEDL
                     EAARPEIRPGDFVIINTGWHHYYADSRKYFIYSPGLYKEAGIWLRERGVKAVGVDQQA
                     LDHPLATAIGWHPEAPSAPLAPWAL"
BASE COUNT          284 a          695 c          678 g          259 t
ORIGIN      
        1 ccttggagta gggcagccgc tcggcgagct gttcgagcac gaccacccgc accccggcga
       61 ggtgcagctc ggcggcgagc cacaggccga ccgggccgcc gcccacgatc acgacgtcgg
      121 ccacggccgc cgccgtgcca ccgccccgca tcccttcgcg catagcgcag tttagccgga
      181 attcagccgc tttcacgtca gcctgatgcg gcggcacggt cgaggcaggt gtagtcgagg
      241 caggtgtagt cgagcgccgc ctcgccgacc cacgcactcc cgctctcggg ctcggcggtc
      301 gctgtggagg cgccgggcgg gagctgcccg ctggcgcgca gcgcccccac cgccgctgcc
      361 agcgccccgg gggtgacccg caggtcgggg gggaggacct gcttgccggc cagctcctgg
      421 caggcccgca gcgcgtggcg gcggggcagc tgggtccggg ccgccgcgat ggcggcggcc
      481 ccttcgacgt cgccgtagaa ccagcgggtc gcctccagca gggcggccag cagcgcgctc
      541 accgcctcgc cgttggcagc gaggtagtcg cggtgggcgc agaccgcgct gaaggcgaac
      601 tccggcaccg aggcgtcggc gtcgccgagg ttgctgaagc cggcctcctg cgccaggtag
      661 ttgtagggca cgagctgcag cgccgcgtcg atcgacccgt cctgcagggc tcgccagcgg
      721 gcctggtggt tgccttcgag ggcgaagtcg taatcgcgcg gccaggtgag gccctcggcg
      781 gccagcatcg cctcggccag gtgcttggtg ccctccttga gcgaggaggt gccgatccgc
      841 ccgccgcgca gatcggcgat ggcgtggtgg cggggcagcg cgatcagcga cagcggcggc
      901 ttgttggcga tgccggcgac gacccggagc gggccgcccg cgacggcatc ggcaatggtc
      961 ccctccggcg cggtcatggc gacgtcgacg gtgcggtcgc gcagcgcggc ggtggtcccc
     1021 tcggtggtgc cgaggatcac gctggtgacg ccgacgccgt ggcgctccag gatgccctcg
     1081 tgctcggcga cccacagcgc gacgccgaca taggccggcg cgacgtggcc gatgatgatc
     1141 tcccgcacgg cgaccaccct cgcttcggag tttcggagcc tggctccgtt gccatccgtt
     1201 ccgttgacaa gccagaagtg ccctgcgatc gttcaacctc gtagcgaacc acgatgcaag
     1261 cgggttgtcc cgcattctag aacatcagga ggggcaaacg tgaccaagca ggccggatca
     1321 agcagcggcc ccgctccggt gatgagccgg gaagagtggc agatgcaccc cgccggccgg
     1381 accggcctcc agttctacga cctgagccac accttcgggc ccaacgcccc cctgtggccg
     1441 tacttcgagg acgtgaagat cgagcggctc cactaccacg ccaagtcagg ggtgctgtcg
     1501 cagcggatca gcacggtgat gcactgcacc acccacctgg acgcccccgc ccacgtggtc
     1561 gagggaaccc cctacaccga cgaggtgccg ctggagcagt tcttcggcac cggcgtggcc
     1621 gtctcgatcc ccaagaagca gtgggaggtc atcactccgg aggacctgga ggcggcccgc
     1681 cccgagatcc gccccggcga cttcgtgatc atcaacaccg gctggcacca ctactacgcc
     1741 gacagccgca agtacttcat ctactccccc ggcctctaca aggaagccgg gatctggctg
     1801 cgggagcgcg gcgtcaaggc ggtcggggtg gaccagcagg cgctcgacca cccgctggcg
     1861 accgcgatcg gctggcaccc ggaggcgccg agcgccccgc tcgccccctg ggcgct
//
LOCUS       DASWDC010000136        13200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1449302, whole genome shotgun sequence.
ACCESSION   DASWDC010000136 DASWDC010000000
VERSION     DASWDC010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13200
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449302"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VF995_04330"
     CDS             complement(<1..354)
                     /locus_tag="VF995_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342827.1"
                     /translation="MRGLVLALSGALTLAMAVAGCGSGAGATGAGSAGSGSAGAPGRP
                     AQAAAAALAFATGSDAEVAVVDGFTALPAVVQRGQQPLSQAGGNTKPCAKAPQGSFTP
                     SERAALQVPFQGRTLR"
     gene            537..998
                     /locus_tag="VF995_04335"
     CDS             537..998
                     /locus_tag="VF995_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX9342828.1"
                     /translation="MTPTPVDDAARLRAAGLRVTAPRLAVIAAVRAGDHAPVDEIAAR
                     ARDRVGSVSTQAVYDVLRALDGAGLVRRVELFGSPARYEARVGDNHHHLVCRACGAIN
                     DVDCAVGHAPCLEPANDAGYLVQEADVTYMGICPTCRTTSSPTSHGTEASA"
     gene            995..2485
                     /locus_tag="VF995_04340"
     CDS             995..2485
                     /locus_tag="VF995_04340"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX9342829.1"
                     /translation="MSERMSDRSERSDGQRPPATTTDAGIPAPSDEHSLTVGPSGPIL
                     LHDHYLIQKMAQFNRERVPERVVHAKGGGAHGFFELTEDASQWCKADFLARRGTRTPV
                     FVRFSTVAGELGSADTVRDPRGFAIKFYTNEGNYDLVGNNTPIFFVRDPQKFQDFIHS
                     QKRRPDTNTRDHNMQWDFWTLSPESAHQVTFLMSDRGTPRTWRHMHGFSSHTFLWVNA
                     GGEKFWVKYHFKSDQGIENFTDAQAKAMTAQDPDFHLRDLYQAIKHGDHPSWTLQMQI
                     MPFDDAWDYRFNPFELTKVWPHGDYPPVPIGRLVLDRNPENYFAEVEQAAFEPANMVS
                     GIGPSPDRMLLGRLFSYPDTHRHRIGTNYLQLPINQPKSPVHSYNKDGAMRFRHNGDQ
                     PVYAPNSYGGPQADPRHEEPGWHASGDLGRCATEPHREDDDFVQPGTLYRQVLSDLDR
                     DHLVTNIATHLRQGVEADIQRHAIEYWRRVDPDLGARVAKLLGNGG"
     gene            complement(2496..2894)
                     /locus_tag="VF995_04345"
     CDS             complement(2496..2894)
                     /locus_tag="VF995_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9342830.1"
                     /translation="MPLRFHGHTINQLLAQARSRLVRLDPAETAEAVEAGALLVDIRP
                     AAQRQAEGEVPGALVIERNVLEWRLDPASSARIPQADGYDLQVIVMCSEGYTSSLAAA
                     SLQDLGLYRATDLTGGFKAWATAGLPTRQG"
     gene            complement(2910..3341)
                     /locus_tag="VF995_04350"
                     /pseudo
     CDS             complement(2910..3341)
                     /locus_tag="VF995_04350"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
     gene            3591..4301
                     /locus_tag="VF995_04355"
     CDS             3591..4301
                     /locus_tag="VF995_04355"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HEX9342831.1"
                     /translation="MATRTARTAHPYDDTAVVDARAPRFLQGTVGVLAAVALLTGWWW
                     LFGLLALQLIAGLVLGRRWCLPCVFYFEVVQPRIGEGRVEDGRPPRFANMLGAGMLSA
                     TFLADLAGLRVVGTVLGVIVMLLALLAATTGLCVGCELYRLLARFRGIHPGTVGAIDL
                     AALPSEDLVNGDTVVQFTHPLCADCHELEQRLAAGPRPLVLVDVSQRPDLARRYHVTV
                     VPTAFAVAPDGTVLERLT"
     gene            complement(4532..5776)
                     /locus_tag="VF995_04360"
     CDS             complement(4532..5776)
                     /locus_tag="VF995_04360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9342832.1"
                     /translation="MNVGMVSTRFAGTDGVSLETAKLAEVLESEGHQVVWFAGELGKR
                     FRPGITCPLAHFEHPINRRLEAAFFRGEPTPPRMMRTLRALTWKLKKALRQFLTDFEV
                     DAVVAQNVLCLPMQLPLGVALTEVLSETGLPAVGHHHDFGWERDRFSKGHDPHLLHTN
                     FPPALRNLRHMVIQSYAKQELRRRRGLDATVLPNVMDFERGPLTSPDGAAFRRAAGLD
                     ARDLVLLQPTRVVPRKSIETTLRLAHELADPAVKVVVTHDDGDEGPDYGRFLRAEADR
                     LQVDLRFVPTRAEPADASERPLLADAYAAANLVAYPSLIEGFGNALLEALFYRCPVLV
                     NRYPVYVRDIAPTGLRCVEIEDCVVTPEAVKEAAACLENRDAWQEITDHNYEVCRGAF
                     SYNVIRERFLPLLPSLPERQAG"
     gene            complement(6085..6942)
                     /locus_tag="VF995_04365"
     CDS             complement(6085..6942)
                     /locus_tag="VF995_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342833.1"
                     /translation="MPGPVPSPTVFALLDAEWATTLAPNPLPARWHHQPALAPQHALT
                     DLLQATEQRGDPAQSDQILAALASFAADAEFPDPLAARTLLQMLLPGVKALARRLAWL
                     GDPAERAAAVTACLYEQIRDYPIQRRPARIAANLLADTQQRLLRANPSARATATQRPL
                     REVSLEALAALGEAAMPPAPAPDPSPAEALLGLLAWAVSDGHLTLAQASLIGQSRIAD
                     VPCQQLGAGAGLGAHSLRRRRQRAEQALARAASGALAEEDLPGLARQSSRLNRVSRRS
                     CLIYRDALV"
     gene            complement(7135..7911)
                     /locus_tag="VF995_04370"
     CDS             complement(7135..7911)
                     /locus_tag="VF995_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX9342834.1"
                     /translation="MRKEPGLKARLRAGETVVGTFLNLGSPLAVEVCGLAGFDWLLLD
                     LEHGLGGEANLLASLLAAENTGAHPVVRVEGPARQRIGRVLDMDAEGIMVPRVESAAE
                     AKEIAAHLRFPPLGDRGVAAGNRFGAFATRADYVDTAPERVAGIVQIESPAGVAAAAE
                     IAAVDGIDVLFIGPRDLSLSLGIMGQTSHPEFRRAVAEVRRACDTAGKATGILLPGPA
                     AVPQALEDGFRFVGVGSDSGFILEGARAAVASVRSRVPAA"
     gene            complement(7950..9860)
                     /gene="iolD"
                     /locus_tag="VF995_04375"
     CDS             complement(7950..9860)
                     /gene="iolD"
                     /locus_tag="VF995_04375"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEX9342835.1"
                     /translation="MDTRTVRLTAAQALVRFLSLQFSERDGASRRLVPAMLGIFGHGN
                     VAGLGQALDQDGATLPYLQARNEQSMVHLAAAFAKERRRLATLACTSSIGPGATNMVT
                     GAALATISRLPVLLLPGDLYASRRQGPVLQQLEHPLAADLSVNDCFRPVARFFDRIVR
                     PEQLIVTLPEAMRVLTSPSETGAVVLALPQDVQAEAYDYPEALFAPRTWPVARPRPEP
                     GPIAAAAELLAAARRPLLIAGGGVRYSEAEAELAALAEAAEIPVAETFGGKGAVTDPA
                     WWALGGLGLEGNPAANRYAAGADVVVCAGTRLTDFITASQSLFQHPEVRFIGINVSAH
                     DAAKQGAIAIVADAREALRALREALAATGRTTTDHKELRHEVEALTGAWQVERAAALA
                     PPADGADPTLTQAQLIGVLQEEAEPGDVILTAAGGPPGDLLKVWDATGGRDCQLEFGY
                     SCMGYELPATLGVRLARPDSRGQVVGLIGDGTFLMQPSELVTAVQEGLDVTIVISENH
                     GFQCIRRLQMNRVGVPFGNEFRARKGPRDGGGQLEGDYLPLDLAAVARGLGARAWHVD
                     GTDELRAALREARGHDGPSVLVVETVPHHDLPPSGAWWDVAPAEVSGEPATQRLRAAY
                     EADRARLQRSYL"
     gene            complement(9882..10631)
                     /locus_tag="VF995_04380"
     CDS             complement(9882..10631)
                     /locus_tag="VF995_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9342836.1"
                     /translation="MTQPEGLDEAVDRSSPVPLYYQVAQRIEAEIEAGRFPPGTHLHN
                     ELTLAEELGVSRPTVRNAISHLADKGLLIKRRGIGTLVAPVQVKRPIALSSLYDDLHQ
                     SGQRPTTRVLAFETVPCPSQAVEALCLAEGTAVLRFERLRLAGERPIALMVNFLPAGV
                     LPQLSRDDLERSGLYGLLRGRGVRLHLANQIIGARRADAREARLLELGSGAPVLTMRR
                     TAYDDAGRPVEYGSHAYPAERYSFEMSLVTR"
     gene            10831..11787
                     /gene="iolC"
                     /locus_tag="VF995_04385"
     CDS             10831..11787
                     /gene="iolC"
                     /locus_tag="VF995_04385"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019075958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEX9342837.1"
                     /translation="MEPFEVVTMGRVGVDLYPEQIGVPLAEVRTFAKSLGGSATNVAV
                     AAARLGHRSAVITKVGDDPFGPYVRQALAGFGVDPRWVGTDPDPWLRTPVVFCEIFPP
                     DHFPLLFYRQPRAPDMNLAPDDLDLGAVAAARLFWTTGTGLSDEPSRGTTLAALAARP
                     RGGDAGGPVTVHDLDHRPSLWRDEAAAGPLAREALGHASVAVGNLSEVAVAVGPREPD
                     DAAKALLDLGVDFAVIKLGPRGVLGMTRDGAVRVPPVPVEVVCGLGAGDAFGGALCHG
                     LLAGWGPERTLRFANAAGAIVASRLACADAMPTVSEVEGALR"
     gene            11784..12776
                     /gene="iolB"
                     /locus_tag="VF995_04390"
     CDS             11784..12776
                     /gene="iolB"
                     /locus_tag="VF995_04390"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX9342838.1"
                     /translation="MTHKLYLPAGSATGDAPGEAVVITPERAGWTWSGLRVLELGPGE
                     ARTLATGGTELAVLPLAGACVVECEGRRFELHGREHVFAGVSDFAYLPIDAEARLRSA
                     HGGRFALPTAVATRRLDPAYGPAAGVPVELRGAGQASRQLNNFLEPSAFPADKLVAVE
                     VLTPAGNWSSYPPHKHDQCRLPEEAQLEEIYYFEVARTGQAPSRDGFGLHRLYTTDGE
                     IDLTEQVRSGDAILIPRGYHGPSVAAPGYDLYYLNVLAGPADQRTMAFCDDPAHTWVR
                     GTWATQAVDPRLPMTGARRRFLAAGAGNSGRSDISQRIEEILRGEATAPGDDSA"
     gene            complement(12932..>13200)
                     /locus_tag="VF995_04395"
     CDS             complement(12932..>13200)
                     /locus_tag="VF995_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342839.1"
                     /translation="PYSQVEGHGLFEVLEALAAGRVAISATPTGPVIMRYGGQLMIVD
                     ASGANLITPEASIRPVRSSLLTLPAGSGLYRLTDPAGAIIALSP"
BASE COUNT         1889 a         4798 c         4686 g         1827 t
ORIGIN      
        1 gcggagtgtg cgcccttgga acgggacctg cagggcggca cgctcggacg gggtgaacga
       61 cccctggggc gccttggcgc agggcttcgt gttcccgccg gcctggctga ggggctgctg
      121 gccacgctgc accaccgcgg gcagggcggt aaagccgtcc accaccgcga cctcggcgtc
      181 gctgccggtg gcgaacgcca gcgctgcggc ggcggcctgg gctggcctgc caggagcgcc
      241 ggccgagccc gacccggccg agccggcccc cgtggcaccg gcaccagaac cacatccggc
      301 cacggccatg gccagcgtga gcgccccgct gagggcgagc acgagtcctc gcatcagtgc
      361 gcctcctcct cgtcctgctc cctgtccctg ccgggtcggc tggttcctcc caatcttcgt
      421 aggtgtccca gattccgtcc gtcaaacaac tagatgtgct cagaagaccg tttaattctg
      481 atcagcgtat aattccttcc ttggaatgat tccagaactg tggtagggtc gccgccatga
      541 caccgacgcc tgttgacgac gccgcccggc tgcgggccgc cgggttgcgg gtcaccgcgc
      601 cgcggctggc ggtgatcgcg gcggtgcggg cgggtgacca cgcgccggtc gacgagatcg
      661 cggccagggc gcgcgaccgg gtcggcagcg tctccaccca ggcggtctac gacgtgctcc
      721 gagcgctcga cggagctggg ctggtccgcc gggtggagct gttcggcagc ccggcacgct
      781 acgaggcccg ggtcggcgac aaccaccacc acctcgtctg ccgcgcctgc ggggcgatca
      841 acgacgtgga ctgcgccgtc gggcacgcgc cctgcctgga gcccgccaac gacgccggct
      901 acctggtgca ggaggcggac gtcacctaca tggggatctg ccccacctgc cggaccactt
      961 cttccccgac ttcccacggc acggaggcga gcgcatgagc gagcgcatga gcgaccgcag
     1021 tgaacgcagc gacgggcagc ggccaccggc gaccaccacc gatgccggga tcccagcgcc
     1081 cagcgacgag cactccctga cggtcggccc cagcgggccg atcctgctgc acgaccacta
     1141 cctgatccag aagatggcgc agttcaaccg cgagcgcgtc cccgagcgcg tcgtccacgc
     1201 caaaggcggc ggcgcgcacg gcttcttcga gctgaccgag gacgcgagcc agtggtgcaa
     1261 ggcagacttc ctggcccgca ggggcacccg cacgcccgtg ttcgtacgct tctcgaccgt
     1321 ggcgggcgag ctgggttcgg ccgacaccgt ccgcgacccg cgcggcttcg ccatcaagtt
     1381 ctataccaac gagggcaact acgacctggt cggcaacaac accccgatct tcttcgtgcg
     1441 cgacccgcag aagttccagg acttcatcca ctcgcagaag cgccgccccg acaccaacac
     1501 ccgcgaccac aacatgcagt gggacttctg gaccctgtca ccggagtccg cccaccaggt
     1561 gaccttcctg atgtccgacc ggggcactcc ccggacctgg cggcacatgc atggcttctc
     1621 cagccacacc ttcctgtggg tcaacgcggg cggcgagaaa ttctgggtga agtaccactt
     1681 caagtccgac cagggcatcg agaacttcac cgacgcccag gccaaggcga tgaccgccca
     1741 ggaccccgac ttccacctgc gcgacctgta ccaggcgatc aaacatggcg accacccctc
     1801 gtggacgctg cagatgcaga tcatgccgtt cgacgacgcc tgggactacc gcttcaaccc
     1861 cttcgagctg accaaggtct ggccgcacgg cgactaccca ccggtcccga tcggccgcct
     1921 ggtgctcgac cgcaatcctg agaactattt cgccgaggtc gagcaggcag ctttcgagcc
     1981 ggccaacatg gtcagtggca tcggcccgag ccccgaccgg atgctgctcg gccggctgtt
     2041 cagctacccc gacacccacc gccaccggat tggcaccaac tacctgcagc tgcccatcaa
     2101 ccagccgaag tcgcccgtcc acagctacaa caaggacggc gccatgcgct tccgccacaa
     2161 cggcgaccag ccggtctacg cgccgaactc ctacggcggc ccccaggccg acccccgcca
     2221 cgaagagccg ggctggcacg ccagcggcga cctcggccgc tgcgccaccg aaccccaccg
     2281 cgaggacgac gacttcgtcc agccaggcac cctctaccgc caggtcctct ccgacctcga
     2341 ccgcgaccac ctggtcacca acatcgccac ccacctccgc cagggcgtcg aggccgacat
     2401 ccagcgccac gccatcgaat actggcgccg cgtcgacccc gacctcggcg cccgcgtcgc
     2461 aaaactcctc ggcaacggcg gctgagcccg cctgcctacc cctgcctggt cggcaggccg
     2521 gcggtggccc aggctttgaa gccgccggtc aggtcggtgg cgcggtaaag gccgaggtcc
     2581 tggagagagg cggcggcgag gctggaggtg tagccctccg agcacatgac gatcacctgg
     2641 aggtcgtagc cgtcggcttg ggggatgcgg gcgctgctgg ctgggtcgag gcgccattcc
     2701 aggacgttgc gttcgatcac caaggcgccg gggacctcgc cctcggcctg gcgctgggcg
     2761 gcgggacgga tgtcgaccag cagggcaccg gcctccaccg cctcggcggt ctcggcgggg
     2821 tcgaggcgga ccagccgcga gcgggcctgg gcgagcagct ggttgatggt gtggccgtgg
     2881 aaacgcagcg gcagcgacgg cggcgagtag gcgtgcacgc tggtcgccac cgctccggcc
     2941 gcgccgaggt tgcggctggc gttggcgacc gaatggatcc agcggggccc gaaccaggtg
     3001 gctgacccgg ctcggtgaac ggtgcgcacc ccctcggcgg actcctgcag cgcaccctcc
     3061 accaccgtga acgcgcccca ggcgccgccg tggtcatgga tccgcacccc ctggccgggg
     3121 gcccagccga gcacccagac ctcgatgctg gcggtccgca gcagccgctc gtagaagcgt
     3181 tggtgcgggt cgtggcgcac gaccggccgc cacagctcct ccagtgcggc cacctggcgg
     3241 gcggcatcga cgagcagctc ggcgtcgagc gtgtcgtcgc cgtcgagcgg cgttcgctcg
     3301 aaggaaccgc gggccggctc cagccgggga tgcaggaaca tacgaagaaa cctccaggca
     3361 gagtgaaacg ttcggagcgt gggacaggat cgctgacgtc agcgatggcg gggacacgcg
     3421 cacgacacga cgcgcccgaa gtcaagcgcg cggtcgcggg tcaaccagcc agtggcccat
     3481 gtgctcatgc caggaagatt ccaggcgttg gcgcttattg tcaagttgct tggtagaaaa
     3541 tggtgcgcag agccgttgac agggtgtccg gattgcggca ccgtcgggac atggccacga
     3601 ggacggcacg gaccgcgcac ccctacgacg acaccgccgt ggtcgacgcc cgcgctcccc
     3661 gcttcctgca gggaaccgtc ggcgtgctcg ccgcggtcgc gctgctgacc gggtggtggt
     3721 ggctgttcgg gctgctggcg ctgcagctga tcgccggcct ggtcttgggg cggcgctggt
     3781 gcctgccgtg cgtgttctac ttcgaggtgg tccagccccg catcggcgag ggcagggtcg
     3841 aggacggccg cccgccccgg ttcgccaaca tgctcggcgc cgggatgctg tcagcgacct
     3901 tcctggccga cctcgccggc ctgcgcgtgg tcgggacggt cctcggcgtg atcgtcatgc
     3961 tgctcgccct gctcgccgcc accaccgggc tctgcgtcgg ctgcgagctg taccgcctgc
     4021 tcgcccgctt ccgcggcatc caccccggca cggtcggcgc gatcgacctg gccgcgctgc
     4081 cctccgagga cctggtgaac ggcgacaccg tcgtccagtt cacccacccg ctctgcgccg
     4141 actgccacga gctggaacag cgcctcgccg ccggcccccg cccgctggtc ctggtcgacg
     4201 tgtcgcagcg acccgatctg gcccgccgct accacgtcac ggtggtcccc accgccttcg
     4261 cggtcgcccc cgacggcacc gtcctggaac gcctcacctg accccccaaa ccacactcga
     4321 cacctcccca ggggtgcact gcgacccata gccccacgag cccccccgga ccacactcga
     4381 cacttttcct aggggtgagc tgcgagcctg cgccgcatgg accaccatcg gcccggacga
     4441 ggtcccgctc acgcgaccta gttccgtagc actgagcggg acctcgtccg ggccaacccc
     4501 gccgcggcgc aggctcgcac ccggggtatc cttaccccgc ttggcgctcg gggagagatg
     4561 ggagcagcgg caggaagcgc tcgcggatga cgttgtagga gaaggcgccg cggcagacct
     4621 cgtagttgtg gtcggtgatc tcctgccagg cgtcccggtt ctccaggcag gcggcggcct
     4681 ctttgaccgc ctcgggggtg accacgcagt cctcgatctc gacgcagcgc agcccggttg
     4741 gagcgatgtc gcgcacgtag accgggtagc ggttgaccag caccgggcag cggtagaaga
     4801 gcgcctccag cagggcgttg ccgaacccct cgatcaggct cgggtaggcg accaggttgg
     4861 cggcggcgta ggcgtcggcg aggagggggc gctcggaggc gtcggccggc tcggccctgg
     4921 tcgggacgaa gcgcaggtcg acctggaggc ggtcggcctc ggcgcgcagg aagcggccgt
     4981 agtcgggacc ctcgtcgccg tcgtcgtggg tgacgaccac cttgacggcg ggatcggcca
     5041 gctcgtgggc gagccgcagc gtggtctcga tgctcttgcg ggggaccacc cgggttggct
     5101 gcagcagcac caggtcgcgc gcgtcgaggc cggcggccct gcggaaggcg gcgccgtcgg
     5161 gggaggtgag ggggccacgc tcgaagtcca tgacgttcgg cagcacggtg gcgtccaggc
     5221 cgcggcggcg gcgcagctcc tgcttggcgt aggactggat caccatgtgg cgcaggttgc
     5281 gcagcgccgg cgggaagttg gtgtggagca ggtgggggtc gtggcccttg ctgaagcggt
     5341 cgcgctccca gccgaagtcg tggtggtggc cgacggccgg caggccggtc tcgctgagca
     5401 cctccgtcag cgccaccccg agggggagct gcatcggcag gcagagcacg ttctgggcga
     5461 ccaccgcgtc cacctcgaag tcggtgagga actggcgcag ggccttcttg agcttccagg
     5521 tgagcgcccg cagcgtccgc atcatccgcg gcggggtggg ttcgccgcgg aagaaggcgg
     5581 cctccaggcg gcggttgatg gggtgctcga agtgcgccag cgggcaggtg atgcctggcc
     5641 ggaagcgctt gccgagctca ccggcgaacc agacaacctg gtgaccctcg ctctcgagga
     5701 cctcggcgag cttggccgtc tccaggctca ccccgtcggt ccctgcgaat cgtgtcgaca
     5761 ccatgccgac gttcatcgtt ctcctcacga tcgttgccag acgttgtccc cggtccaaga
     5821 cgcgtcaagg cttgccggtg atgcatcgcc ccagacggtc attcggtcat tcacaaccag
     5881 aaagtaagga gcctaatcgc atctggctgt cgttctcttg attcatccgc tgtatccgtg
     5941 ctcgggagag atgcctggtc aattccggcc acatcacatg cggccaggta gttggaaccc
     6001 cgcacggccg tgaatacctg cggggaatga ctgaggggag cgcccgcact acggacactc
     6061 cccactcgac cactgaagct cgcgtcacac tagagcatca cgataaataa gacaagatct
     6121 tcgggatact cgattcagcc tgctgctctg ccgtgccagg ccggggaggt cctcctccgc
     6181 cagggcgccg ctggcggccc gtgccagggc ctgctcggca cgctggcggc ggcggcgcag
     6241 gctgtgggcg cccagcccgg cgccggcgcc gagctgctgg cagggcacgt cggcgatgcg
     6301 gctctggccg atcaggctcg cctgcgccag ggtcaggtgc ccgtccgaga ccgcccaggc
     6361 cagcaggccg agcagcgcct cggccggcga cgggtccggc gccggcgcgg gtggcatggc
     6421 cgcttcgccg agggctgcca gcgcctccag cgacacctcc cgtagcgggc gctgcgtcgc
     6481 agtcgcacgg gcgctggggt tggcgcgcag gagccgctgc tgggtgtcgg cgagcaggtt
     6541 ggcggcgatg cgggcggggc gccgctggat cgggtagtca cggatctgct cgtacaggca
     6601 ggcggtgacc gccgccgccc gctccgccgg gtcgccgagc caggccagcc gtcgggcgag
     6661 cgccttgacg ccgggcagca gcatctgcag cagggtcctg gcggccaggg ggtcggggaa
     6721 ttcagcgtcg gcggcgaagg acgccagggc ggcgaggatc tggtcgctct gggcggggtc
     6781 gccgcgctgc tcggtcgcct ggagcaggtc ggtgagggcg tgttgggggg cgagcgcggg
     6841 ctggtgatgc cagcgggcgg gcagcgggtt cggggccagg gtggtggccc attcggcgtc
     6901 cagcaacgcg aacaccgtcg ggctcggcac aggaccgggc atggctgggg gtcctttcgg
     6961 gcgggggcgg ttggcccacc ctcgcccgtc cggcttgccg cgcatccgac agcgcgcctg
     7021 tcaacgcgcg gattgatcga aggttttccc aggtcagccc ttcagacagc gacctgtatg
     7081 gtgacccggc cctgtcagtt caagtgtcag ctccggtgtc ggccgctggt cggctcaggc
     7141 tgccggtacg cgggagcgga ccgaggcgac cgccgccctg gcgccctcca ggatgaagcc
     7201 gctgtcggag ccgacgccga cgaagcggaa gccgtcctcc agcgcctgcg gcacggcggc
     7261 cgggccgggg agcaggatgc cggtggcctt gccggccgtg tcgcaggcgc ggcgcacctc
     7321 cgcgacggcg cggcggaact ccgggtggct ggtctggccc atgatgccga gcgacaggga
     7381 gaggtcgcgg ggcccgatga agagcacgtc gatgccgtcc acggcggcga tctcggcggc
     7441 ggcggcgacc ccggccgggc tctcgatctg cacgatcccg gcgacccgct cgggcgcggt
     7501 gtcgacgtag tcggcgcggg tggcgaaggc gccgaagcgg ttgccggcgg ccacgccccg
     7561 gtccccgagc ggcgggaaac gcaggtgcgc ggcgatctcc ttggcctcgg cggcgctctc
     7621 gacccgtggg accatgatcc cttcggcgtc catgtcgagc acccggccga tgcgctgccg
     7681 cgccggccct tcgacccgca cgaccgggtg ggcgccggta ttctcggcgg cgagcaggct
     7741 ggcgagcagg ttggcctctc ccccgaggcc gtgctccagg tcgagcagca gccagtcgaa
     7801 cccagccagc ccgcagacct cgaccgccag cggcgacccg aggttcagga aggtgccgac
     7861 caccgtctcg ccggcccgca ggcgtgcttt gagccctggt tcctttcgca tggacaactc
     7921 ctcacaaatc ggcttcctca agcggaagcc tacaagtagc tccgctgcaa ccgggcgcgg
     7981 tcggcctcgt acgcggcacg cagccgctgc gtggccggct caccggacac ctcggcgggc
     8041 gccacgtccc accacgcccc cgagggcggc aggtcatgat gcgggacggt ctcgaccacg
     8101 agcaccgacg gcccgtcgtg gccgcgcgcc tcccgcaggg ccgcccgcag ctcgtcggtg
     8161 ccgtccacgt gccaggcgcg cgcgcccagc ccgcgcgcca ccgccgccag gtcaagcggg
     8221 aggtagtcgc cctccagttg gccgccccca tccctcgggc ccttgcgggc gcggaactcg
     8281 ttgccgaacg ggaccccgac ccggttcatc tgcaggcggc ggatgcactg gaagccgtgg
     8341 ttctcgctga tcacgatggt cacgtccagg ccctcctgga cggcggtgac cagctcggac
     8401 ggctgcatca ggaaggtccc gtcgccaatc agcccgacca cctggccgcg gctgtccggc
     8461 cgcgccaggc gaaccccgag ggtggcgggc agctcgtagc ccatgcagga gtagccgaac
     8521 tccagctggc agtcccgccc gccggtggcg tcccagacct tgagcaggtc cccgggcggg
     8581 ccgcccgcgg cggtcaggat cacgtcgccg ggctcggcct cctcctggag gacgccgatc
     8641 agctgggcct gggtgagcgt cgggtccgct ccgtcggcgg gaggggccag cgcggcggcc
     8701 cgctcgacct gccaggcgcc ggtcagcgcc tcgacctcgt gccgcagctc cttgtgatcg
     8761 gtggtggtgc ggccggtcgc ggcgagcgcc tcgcgcagcg cgcgcagcgc ctcgcgggcg
     8821 tcggcgacga tcgcgatggc gccctgcttg gcggcgtcgt gggcgctcac gttgatgccg
     8881 atgaagcgga cctccggatg ctggaacagc gactgggagg cggtgatgaa gtcggtcagg
     8941 cgggtgccgg cgcacaccac gacgtcggcg ccggcggcgt agcggttggc ggccgggtta
     9001 ccctccagcc cgaggccgcc aagcgcccac caggccgggt cggtgacggc gcccttgccg
     9061 ccgaaggtct cggcgaccgg aatctccgcc gcctcggcga gggcggccag ctcggcctcg
     9121 gcctcgctgt agcggacccc gccgccggcg atcagcagtg ggcgcctggc ggcggcgagc
     9181 agctcggcgg ccgcggcgat cgggccgggc tccgggcggg ggcgggccac cggccaggtg
     9241 cggggggcga acagcgcctc ggggtagtcg taggcctcgg cctgcacgtc ctggggcagg
     9301 gcgaggacca ccgcgccggt ctcgctgggg ctggtgagca cgcgcatcgc ctcgggcagg
     9361 gtgacgatca gctgctcggg ccgcacgatc cggtcgaaga aacgggcgac cgggcggaag
     9421 cagtcgttga cgctaagatc ggcggccaat ggatgttcca gctgctgcag caccgggccc
     9481 tgccggcggc tggcgtacag gtcgccgggg agcagcagca ccggcaggcg gctgatggtg
     9541 gccagggccg cgccggtgac catgttggtc gcccccggcc cgatcgagga ggtgcacgcc
     9601 agcgtggcga gccggcggcg ctccttggcg aaggcggcgg ccaggtggac catcgactgc
     9661 tcgttgcgcg cctgcaggta gggcagggtg gcaccatcct ggtcgagcgc ctgcccgagc
     9721 ccggcgacgt tgccgtgccc gaagatcccg agcatcgccg gcaccagccg cctgctggcg
     9781 ccgtcacgct cgctgaactg cagcgacagg aagcggacca gcgcctgcgc ggcggtgagc
     9841 cgcacggtcc gggtgtccat cacgaaccct ccaaaccacc gctagcgggt gaccaggctc
     9901 atctcgaagg agtagcgctc ggcggggtag gcgtgcgagc cgtactcgac cggccgcccg
     9961 gcgtcgtcgt aggcggtgcg ccgcatggtc aggaccggcg cgccgctgcc aagctccagc
    10021 agccgggcct cgcgggcgtc ggcgcggcgc gcgccgatga tctggttggc gaggtggagg
    10081 cgcacgccgc ggccgcgcag cagcccatag aggccgctgc gctccaggtc gtcgcggctg
    10141 agctgcggca gcacgccggc cggcaggaag ttgaccatca gggcgatcgg ccgctccccg
    10201 gcgaggcgca ggcgctcgaa gcgcagcacc gccgtcccct ccgcgaggca gagcgcctcg
    10261 accgcctgcg acgggcaggg gaccgtctcg aaggcgagca cccgggtggt cggccgctgg
    10321 ccgctctggt gcaggtcgtc gtacaggctg ctgagggcga tcgggcgctt gacctgcacc
    10381 ggcgcgacca gggtgccgat cccccggcgc ttgatcagca ggcccttgtc ggccaggtgg
    10441 ctgatggcgt tgcgcaccgt cgggcggctg acgcccagct cctcggccag cgtcagctcg
    10501 ttgtgcaggt gggtgcccgg cgggaagcgg cccgcctcga tctccgcctc gatccgctgg
    10561 gcgacctggt agtacaacgg cactggactg gaacggtcga cagcctcgtc cagaccctca
    10621 ggttgggtca tgcacgcacc gtcctgattg ccggaggcac ggtcgttatg acatcctttc
    10681 atcattacct aggcggccgc agctccaatg ttgccctgtc cgcccaaccg gcacaagccc
    10741 gatctcgtgg tccccgccgg ccgagcgtgc aggcgagcgg agcagcgccg tccacgggag
    10801 gctggtggag gcggaggcgg gaggtgcgcc atcgagccgt tcgaggtcgt caccatggga
    10861 cgggtcgggg tggacctcta cccggagcag atcggcgtgc cgctcgccga ggtccgcacc
    10921 ttcgccaagt cgctcggcgg cagcgccacc aacgtcgcgg tcgctgcagc ccgccttggc
    10981 caccgcagcg cggtcatcac caaggtcggc gacgacccct tcggccccta cgtgcgccag
    11041 gcgctggccg gcttcggcgt cgatccccgc tgggtcggca ccgacccgga cccgtggctg
    11101 cggaccccgg tggtgttctg cgagatcttc ccgcccgacc acttcccgct cctgttctac
    11161 cgccagccca gggcgccgga catgaacctc gcgcccgacg acctggacct cggcgcggtc
    11221 gccgccgccc ggctgttctg gaccaccggc accggcctgt ccgacgagcc gagccgcggc
    11281 accaccctcg ccgccctcgc cgcccggccc cgcggcgggg acgcgggcgg tccggtcacc
    11341 gtccacgacc tggaccaccg cccgtcgctg tggcgcgacg aggccgccgc cgggccgctc
    11401 gcccgcgagg cgctcggcca tgccagcgtc gcggtcggca acctgagcga agtggcggtc
    11461 gcggtcggcc cgcgcgaacc cgacgacgcc gccaaggccc tgctcgacct cggcgtcgac
    11521 ttcgcggtca tcaagctcgg cccgcggggg gttctcggca tgacccgcga cggcgccgtg
    11581 cgggtccccc cggtcccggt cgaggtggtc tgcggcctcg gcgccggcga cgccttcggc
    11641 ggcgccctct gccacggcct gctcgccggc tggggccccg agcgcaccct ccgcttcgcc
    11701 aacgcggcgg gcgcgatcgt cgcctcccgc ctcgcctgcg ccgacgccat gccaaccgtc
    11761 tccgaggtgg agggcgccct ccgatgaccc acaagctcta cctgcccgcc gggtccgcca
    11821 ccggggacgc gcccggggag gccgtggtga tcacccctga gcgcgccggc tggacctggt
    11881 ccgggctgcg ggtgctggaa ctgggccccg gcgaggcgcg caccctcgcc accggcggca
    11941 ccgagctggc cgtgctcccg ctcgcgggcg cctgcgtggt cgaatgcgaa ggccgccgct
    12001 tcgagctgca cggccgcgag cacgtcttcg ccggggtgag cgacttcgcc tacctgccga
    12061 tcgacgcgga ggcgcgcctg cgcagcgccc acggggggcg gttcgcgctc cccaccgccg
    12121 tcgcgacccg ccgcctcgac ccggcctacg gcccggccgc cggggtcccg gtggagctgc
    12181 gcggcgccgg ccaggcaagc cgccagctca acaacttcct ggagccgtcg gcgttcccgg
    12241 ctgacaagct ggtcgccgtc gaggtcctca cccctgcggg caactggtcc tcctacccgc
    12301 cccacaagca cgaccagtgc cgcctccccg aggaggccca gctcgaagag atctactact
    12361 tcgaggtggc ccgcaccggc caggccccca gccgcgacgg cttcggcctg caccgcctct
    12421 acaccaccga cggcgagatc gacctcaccg agcaggtccg cagcggcgac gccatcctga
    12481 tcccccgcgg ctaccacggc ccctcggtcg ccgcccccgg ctacgacctc tactacctca
    12541 acgtcctcgc tggccccgcc gaccagcgca ccatggcctt ctgcgacgac cccgcccaca
    12601 cctgggtccg aggcacctgg gcgacccagg cggtcgaccc ccgcctcccc atgaccgggg
    12661 ctcgacggcg cttcctcgcc gcgggtgccg gcaacagcgg acggtctgac atctcccagc
    12721 gaatcgagga gattcttcga ggggaggcga ccgctcctgg tgacgactca gcctgaacgg
    12781 cgcagcaacg ggagcagaac agctcgccag tctggacgac gtgcggagac tccgctctca
    12841 tccagcagca tccagacgat gtcagccagg ctgacgatgc cggtgggtgc gactaaccct
    12901 accacgctca tacccgacaa aacaggctgt ctcatggaga gagcgcaatg atggcaccgg
    12961 ctgggtcggt gaggcggtag agcccggagc cggccgggag ggtgaggagg ctggagcgga
    13021 ccggccggat tgaggcttca ggggtgatga ggttggcgcc agaggcgtca acgatcatga
    13081 gttggccgcc gtagcgcatg atgaccgggc ctgtgggagt cgcggagatg gcgacccggc
    13141 cggctgcgag ggcttcgagc acctcgaata agccgtgacc ctcaacctga gagtaaggat
//
LOCUS       DASWDC010000137         4555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1284638, whole genome shotgun sequence.
ACCESSION   DASWDC010000137 DASWDC010000000
VERSION     DASWDC010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284638"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1046)
                     /locus_tag="VF995_04400"
     CDS             complement(69..1046)
                     /locus_tag="VF995_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEX9342840.1"
                     /translation="MVAHKLVGGGAQVVVVQLEPGAMVWCQPGAFLWKTPNVQVQTRV
                     IGAGAGAGAGGGVGLLDRALATAVTAGMRRLAGQAAALPSFSVPDGGSGLVAFASRRP
                     GAGAIRALRLEGGRGWLVASGALVAAEAGVGFALGERPAAGFERLGGRGTAFVCGRGS
                     LLALDLARYGGSLDVDHARLVAVQDGVRVETPQARSDTPGAGAGALLTAVLGGGPLSV
                     ARLTGEGLALLEAAGAAAEAAAAAARPGAGAGAGGEGGAVDPPISILPPGGRRARRPA
                     PWSRGERAPRMERVLHPEPDRDPEVQADPEPHEPEPHDEGEGGPNGVPR"
     gene            1177..2559
                     /locus_tag="VF995_04405"
     CDS             1177..2559
                     /locus_tag="VF995_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate phosphoribosyl transferase"
                     /protein_id="HEX9342841.1"
                     /translation="MQDASPAMPHMPHLGAGEAATPLPIAGPELRGADGRLASWIFDL
                     PVTELRAGYRSAVYFDRTRRILAAEDDDTEVTMQVFQRGDGVVACGIDEAIAVLALGA
                     GAPTDQAAADQAFADYLHARDRVRRSRLAGGAAYLEAVRAQVEAEVAFDRLWRPVQSD
                     ELRLRALRDGERVAAWGPVVEITGPYRLFAHLESVYLGVLARRTLVATNVSRVVDAAG
                     KKPVLFFADRFDHWATQGGDGYAAFVGGAQGVATDAQAAWWGERGLGTIPHALIATFN
                     GSTVAAVAAFARHIPDVPLIALVDFENDSVRTSLACARAFGERLWGVRLDTAQTMVDR
                     SLWQQLGEFTPTGVNPRLVRNVREALDAEGFGHVRVIVSGGFNAERIAAFEAAGVPVD
                     SYAVGSSLLKGDCDYTADVVLREGQPCAKVGRHYRPNDQLEPVDLDIHDGGRPGAESG
                     GPEAGVTEIR"
     gene            2662..3639
                     /locus_tag="VF995_04410"
     CDS             2662..3639
                     /locus_tag="VF995_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017597792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9342842.1"
                     /translation="MQFRNLGSGGPELSVVGFGAWAIGGPWQYGWGPQDDDESLAALH
                     RAFDLGINWVDTAAVYGLGHSEEVVGQAVREHGTDVFIFTKCGLNWYGRADREPQNDL
                     RPESIRFELEQSLKRLGTDHVDLYQFHWPDTATGTPVEESWATMADLVAEGKVRWAGV
                     CNFDVELLERCEPIRHVDSLQPPLSLLDRAALRDLLPWCERHGSGVIAYSPMASGLLT
                     GGFDAERMRALAADDWRHQNPDFQEPRLGRNLALVERLQPIAERHGTTVAAVSVAWVI
                     DQQGSTGAIVGARRPSQIDGWIAAGTLRLSAQDLDEIAAALQVTGAGQD"
     gene            4086..>4555
                     /locus_tag="VF995_04415"
     CDS             4086..>4555
                     /locus_tag="VF995_04415"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9342843.1"
                     /translation="MKAPRKTSPSAERRTYVLDTNVLIADPEAPSRFDEHDVVIPLTV
                     VEELDKLKTRVDETGGSARRAIRVLEDLRAQGNLSQGVGLPGGGCVWVEVNPAARPSL
                     PEGLAADTADNRILATTANLAKELAGERQVVLVSKDASLRIKAEAIKLTAEEYR"
BASE COUNT          638 a         1710 c         1620 g          587 t
ORIGIN      
        1 tccgttcgct gacctgctgc accgcctggt cgaggctgcg ctgcgcctgc ccggcaaagg
       61 ccttcgcgtc atcgaggaac gccattgggt cctccttctc cttcatcatg aggctcgggt
      121 tcatgaggct cggggtcagc ctggacctca gggtcacggt caggctcagg atgcagtacc
      181 cgttccatgc gcggcgcacg ctcgccacgg ctccaggggg ctggccgccg cgcccgccgc
      241 cctcccggtg gaaggatcga gatgggcggg tcgacggctc ctccttcgcc gccggccccg
      301 gcgccggcgc cggggcgcgc ggcggccgcc gcggcctcgg ccgccgctcc cgccgcctcc
      361 agcagcgcca ggccctcccc ggtcagcctg gcgacgctca gcggcccgcc gcccagcacc
      421 gcggtcagca acgcgcccgc gccagctcca ggcgtgtcgg agcgggcctg gggggtctcc
      481 acccggacgc cgtcctggac ggcgaccagg cgggcgtgat ccacgtcgag gctgccgccg
      541 tagcgggcga ggtcgagcgc cagcaggctg ccacggccgc agacgaaggc ggtgccgcgc
      601 ccgccgagcc gctcgaaccc ggcggctggc cgctcgccga gggcgaagcc gaccccggcc
      661 tcggcggcga ccagcgcccc ggacgccacc agccaccccc gcccgccctc cagccgcaac
      721 gcccggatcg ctccggcccc agggcgacgg ctggcgaagg cgaccagccc gctgccgccg
      781 tcgggcaccg agaagctcgg cagcgccgcc gcctgcccgg cgaggcggcg catcccggcg
      841 gtcaccgccg tggccagcgc ccggtccagc agccccacgc cgccacccgc cccggcgccc
      901 gcgcccgcgc cgatcacgcg ggtctgcacc tgcacgttcg gggttttcca caggaacgca
      961 cccggctggc accaaaccat cgcaccaggt tcgagctgga ccaccacgac ctgcgcgccg
     1021 ccgccaacca gcttgtgcgc caccatggcg cgcctcccgt cccgttcggt tccccttcga
     1081 cgctaccttc cacctgcggg ttcggcaacg gacaccaggc ggcgaatcag gcgccgccgg
     1141 cggagggagg cgcccggttc cgctactgtt cctggcatgc aagacgccag tccggccatg
     1201 ccgcacatgc cgcacctggg cgccggcgag gcggcgacgc cgctgccgat cgccggtccg
     1261 gagctgcgcg gcgccgacgg gcggctggcg tcgtggatct tcgaccttcc cgtgaccgag
     1321 ttgcgggccg ggtaccggtc ggccgtctac ttcgaccgca cccggcgcat cctcgcggcc
     1381 gaggatgacg acaccgaggt caccatgcag gtgttccagc gcggcgacgg ggtggtcgcc
     1441 tgcggcatcg acgaggcgat cgcggtgctc gccctcggcg ccggggcgcc caccgaccag
     1501 gccgccgccg accaggcgtt cgccgactac ctgcacgccc gcgaccgggt gcgccgctcg
     1561 cggctggctg gcggcgccgc ctacctggag gcggtccgcg cgcaggtcga ggccgaggtc
     1621 gccttcgacc gcctctggcg gccggtccag agcgatgagc tgcggctgcg ggcgctgcgc
     1681 gacggcgagc gggtcgccgc ctgggggccg gtggtcgaga tcaccggccc ctaccggctg
     1741 ttcgcccacc tggagagcgt ctatctgggc gtgctggcgc gccgcacgct ggtcgccacc
     1801 aacgtcagcc gggtggtcga cgcggccggc aagaagccag tgctgttctt cgccgaccgc
     1861 ttcgaccatt gggccaccca gggcggcgac ggctacgccg ccttcgtggg cggcgcccag
     1921 ggggtcgcca ccgacgccca ggccgcctgg tggggggagc gcgggcttgg caccatcccg
     1981 cacgcgctga tcgcgacctt caacggcagc accgtcgccg cggtcgccgc cttcgcccgc
     2041 cacatcccgg acgtgccgct gatcgcgctg gtcgacttcg agaacgactc ggtccgcacc
     2101 tcgctggcgt gcgcccgcgc gttcggcgag cggctctggg gggtgcggct cgacaccgcc
     2161 cagacaatgg tcgaccgctc gctgtggcag cagctggggg agttcacgcc gacgggggtc
     2221 aacccccggc tggtgcggaa cgtgcgcgag gcgctcgatg ccgaggggtt cgggcacgtc
     2281 cgggtgatcg tctccggcgg cttcaacgcc gagcggatcg ccgccttcga ggccgcgggg
     2341 gtcccggtcg actcctacgc ggtcgggtcc tcgctgctca agggcgactg tgactacacc
     2401 gctgacgtgg tcctgcggga gggccagccg tgcgccaagg tgggccgcca ctaccgcccg
     2461 aatgaccagc tcgaaccggt tgatctggat attcatgacg gagggcgccc cggagcggaa
     2521 tcgggcggcc cggaagccgg tgtcacagag attcggtagg gtggaccccg ggcatccccg
     2581 aatcacgtca tcaccgtcgc cggcatgtcg ctggaccccg gtcccgattt ggcgctgaca
     2641 cggcgaggag gacgcagacg catgcagttc cgcaacctgg gaagtggcgg accagaactc
     2701 tcggttgtcg ggtttggcgc ctgggcgatc ggcgggccct ggcagtacgg gtggggcccg
     2761 caggacgacg acgagtcgct cgcggcgctg caccgcgcct tcgacctggg gatcaactgg
     2821 gttgacaccg ccgcggtcta cgggctcggc cactccgagg aggtcgtcgg ccaggcggtg
     2881 cgcgagcacg gcaccgacgt cttcatcttc accaagtgcg ggctgaactg gtacggccgc
     2941 gccgaccggg agccccagaa cgacctacgg ccggaatcga tccgtttcga gctggagcag
     3001 agcctcaaac gcctcgggac cgaccacgtc gacctgtacc agttccactg gcccgacacg
     3061 gccaccggca cgccggtgga ggagtcctgg gcgaccatgg ccgacctggt cgccgagggc
     3121 aaggtccgct gggcgggggt gtgcaacttc gacgtcgagc tgctggagcg ctgcgagccg
     3181 atccgccacg tcgattcgct gcagccgccg ctcagcctgc tggaccgcgc ggcgctgcgc
     3241 gacctgctgc cgtggtgcga acgccacggc agcggcgtga tcgcctacag cccgatggcc
     3301 tccggcctgc tcaccggcgg ctttgacgcc gagcggatgc gggcgctggc cgccgacgac
     3361 tggcgccacc aaaaccccga cttccaggag ccgcgcctgg gccgcaacct cgcgctggtc
     3421 gagcgcctcc agccgatcgc cgaacgccac ggcaccaccg tcgccgcggt ctcggtggcc
     3481 tgggtgatcg accagcaggg cagcaccggc gcgatcgtgg gcgcccgccg gccctcccag
     3541 atcgacggct ggatcgccgc cggcaccctg cgcctcagcg cccaggacct ggacgagatc
     3601 gccgccgcgc tccaggtgac cggcgccggc caggactgag gccctcccaa tccaaagaaa
     3661 acgtgagggc cgcggtggct ccgcgaccct cacacgtcgt cctcgcctga aagggcgacc
     3721 agatacagcg tggttgcgtc gtccccacac ccggggtggt aaacctccgg ttccccccca
     3781 aagacggccg ctccggtcgt ggcccgcgcc gcgagccacc ggagaagtgg ccggaccagc
     3841 cgccctggga gacgctcctg ggaacaggcg gagcgttctg ctgaagccta gcctaggatg
     3901 actagtccct caagttccag cagaatcagg cacaagcgcg tggccctcgg gtggcggctg
     3961 tctcgccctc ccgggtgccg cgcgctaccg tgaatttggc ccgaggccga gcaaggagcg
     4021 acgcagatgc gtagagcatc ccggctagcg cagcgcgtgt ggcggacgac gagcggggcg
     4081 cgtccatgaa agcaccgcgc aagacgagcc ccagtgccga gcgtcgcacc tacgtgcttg
     4141 acaccaacgt gctgatcgcg gacccggagg cgccgtcgcg cttcgatgag cacgacgtgg
     4201 tcatcccgct caccgtggtc gaggagctgg acaagctcaa gacccgcgtg gacgagacgg
     4261 gcgggtcggc gcggcgggcg atccgggtgc tcgaggacct gcgggcgcag ggcaacctct
     4321 cgcagggggt ggggctgccc ggcggcgggt gcgtctgggt ggaggtcaac ccggcggccc
     4381 ggccgagcct cccggaggga ctggccgccg acaccgccga caaccgcatc ctcgccacca
     4441 ccgccaacct cgccaaggag ctcgccggcg agcgccaggt ggtgctggtc tccaaggacg
     4501 cgagcctgcg catcaaggcc gaggcgatca agctcaccgc cgaggagtac cgcca
//
LOCUS       DASWDC010000138         8336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_534003, whole genome shotgun sequence.
ACCESSION   DASWDC010000138 DASWDC010000000
VERSION     DASWDC010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534003"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VF995_04420"
     CDS             <1..872
                     /locus_tag="VF995_04420"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEX9342844.1"
                     /translation="DQSGIPYAVMGGIAASALGRDRWTHDVDLIVRAEDATDLLERYA
                     ADGFATEERDQDWLYKAFRQGVMIDLIFQVATGAVEEPITLDEEMCQRVRRVVFHGVD
                     MPVLAPEDLAVIKALVHKEHRARHWFDALGLLAAGGIDWTYLLRRARRGPCRVLSLLL
                     YARSDGIEVPSHAIKALLGQVPLDGERAADGTPAPAPAAAPALTAAPAEADPYLVARV
                     LDRLARDSRTAELEVDVRLEQDAVVLTGCVATPERQAALDEVVHTVLPGRPVRNLTTA
                     GPPPAGPRVERLP"
     gene            869..1600
                     /locus_tag="VF995_04425"
     CDS             869..1600
                     /locus_tag="VF995_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010033563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9342845.1"
                     /translation="MIRIAAVGDVHAGVDSTGRLRPGFEGLGDQADLLLLAGDLTHRG
                     RAEEAKVLADELDGLGVPVVAVLGNHDYHSDQQDEVAQVLADSGTQVLEGDAITIQVA
                     GRRLGIAGTRGFGGGFAGACASDFGEPEMKTFTRLTKRLAAQLEGALAGLRDTSDFRV
                     VLLHYAPVAATLAGEPCEIHAFLGSYLLAEAVDRAGADLVLHGHAHRGSRAGTTPGGV
                     PVRNVAQPVIGRAYEVFCLDVDTAP"
     gene            complement(3202..3918)
                     /locus_tag="VF995_04430"
     CDS             complement(3202..3918)
                     /locus_tag="VF995_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9342846.1"
                     /translation="MERLVDLDLGGLRMRTLSVRDEDAALLAEATREETAPALWGPRP
                     VGPYSLEAAWSALRAWDPDQARQVSFGVLDGPRLVAALGLMPDSPHSSSAELAYWVRP
                     EQRRRGIAVRSVQALTLWAHTTAGLSRVWLEIDPDNLASLQVARRAATTRSSACRATA
                     APGSMMIQHRTPGTTASSGPTRPDDLAGAGAPEPRFANPYRSTTAPQVVWWKASPVTG
                     GLEETHQTRAALVGVAATPM"
     gene            complement(4070..4143)
                     /locus_tag="VF995_04435"
     tRNA            complement(4070..4143)
                     /locus_tag="VF995_04435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4107..4109),aa:Arg,seq:tct)
     gene            complement(4236..4535)
                     /locus_tag="VF995_04440"
     CDS             complement(4236..4535)
                     /locus_tag="VF995_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342847.1"
                     /translation="MLNFTRRPEATPELHRFSPLADPVPAYGVGVELTETGMWLRIQH
                     PDGKTESAFVSAPWLRWLAASSVATRAEGFELMKQRTNAASTTTADTRVLRPIGV"
     gene            4813..5997
                     /locus_tag="VF995_04445"
     CDS             4813..5997
                     /locus_tag="VF995_04445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9342848.1"
                     /translation="MADGAAGTRPKVRLGVIYAAHGANHAFVLVLPAVLVSLRAEFGA
                     SFTTLGTVATVSTILYGLGALPAGLLADRFGPPQLLRLFAALSIVCCVLAALAPGIWW
                     LAAALALLGAAGAFYHPAGLAELTLTAPGQGRVLGLHGSAGNLGTALAPLLAGAVASA
                     WTWRGSYALAGIVAAVLLLAVRRGVPLDRQLPPADQAGPRERNRPVLWLVMFLAVAEG
                     FIYQGFVVFLPAFLAAVGHFNRAAAAKGGALSALVLLLGVPGQYFGGRIGEAGPRGLA
                     VRYAALYGGAAVVGQAVHWAGASLLGVVLASVFSLLIFLGQPLTNQLVARGTSAGRRG
                     AAYGGYFSLSFGVGAFAGTAGGVVADHAGLAAVFALLGLVALVNVCGGLTVRALLARE
                     AV"
     gene            6041..6487
                     /locus_tag="VF995_04450"
     CDS             6041..6487
                     /locus_tag="VF995_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX9342849.1"
                     /translation="MATADRRAHALWEGSLTEGKGDVEFASSGIGRYPITWASRVERP
                     DGRTSPEELLAASHAACYAMAFSYALGGAGHSPERLHVTATVTLDPKEGGGFHVTRSA
                     LEVQGRVPGLDQAGFQQLAEQAEQGCPISNALRGNLAITVTATLES"
     gene            complement(6502..7068)
                     /locus_tag="VF995_04455"
     CDS             complement(6502..7068)
                     /locus_tag="VF995_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9342850.1"
                     /translation="MGVRRSVTTWTLEMRRPEQLRPARRVEGLAVERAWEPVPELSRF
                     FYTAVGGDWYWTDRLGWSYADWQRWVGRDEYELWLGCWRGTPVGYLELERQPGPQIEL
                     AYFGLLPRFVGRGFGGQLLSAGVERAWAHGPSRVWVHTCSLDGPQAKRNYEARGFEVC
                     GVETAEQELAERPPGPWPGARATTAAAR"
     gene            complement(7150..7223)
                     /locus_tag="VF995_04460"
     tRNA            complement(7150..7223)
                     /locus_tag="VF995_04460"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7189..7191),aa:Gly,seq:tcc)
     gene            complement(7252..8031)
                     /locus_tag="VF995_04465"
     CDS             complement(7252..8031)
                     /locus_tag="VF995_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9342851.1"
                     /translation="MAQVTSYAAGTPSWVDLMTSDPEGARGFYGQLFGWEYDIGPAET
                     GHYAICKLHGQNVAGIGGETAPQGMTTAWTTYIATDDADGAAKRVHDAGGRLNMEPMD
                     VLDQGRMAIAADPTGAVFGLWQARSHIGASLVNEPGAMIWNELATRDLDAARAFYTAV
                     FGYDWEDVDTGGGPRYATFLVGGRMVGGALQMNEQFPADTPSHWTVYFVVEDTDATAS
                     TAQRLGGGVNSPPMDSPYGRFTNLRDPQGGAFTALRVPDES"
BASE COUNT         1176 a         3168 c         2792 g         1200 t
ORIGIN      
        1 ccgaccagtc cgggatcccc tacgcggtga tgggcgggat cgccgcctcg gcgctcggcc
       61 gtgaccgctg gacccacgac gtcgacctga tcgtccgcgc cgaggacgcc actgacctgc
      121 tggagcgcta cgccgccgac ggcttcgcca ccgaggagcg cgaccaggac tggctctaca
      181 aggcgtttcg ccagggcgtc atgatcgacc tgatcttcca ggtagccacc ggcgcggtcg
      241 aggagccgat caccctcgac gaggagatgt gccagcgggt ccgccgggtc gtcttccacg
      301 gcgtcgacat gccggtgctg gcccccgagg acctggcggt gatcaaggcg ctggtgcaca
      361 aggagcaccg cgcccgccac tggttcgacg ccctcgggct gctcgccgcc ggcgggatcg
      421 actggaccta cctgctgcgc cgcgcccggc ggggcccgtg ccgggtcctc agcctgctgc
      481 tctacgcgcg ctccgacggc atcgaggtcc ccagccatgc catcaaggcg ctgctcggcc
      541 aggtgccgct cgacggcgag cgcgcggcgg acggcacccc agccccagcc ccagccgcgg
      601 cgccagccct caccgcggcc ccggccgagg ccgacccgta cctggtcgcc cgggtcctcg
      661 accggctcgc ccgcgactcc cgcaccgccg agctcgaagt cgacgtgcgc ctcgaacagg
      721 acgcggtggt gctgaccggc tgcgtcgcca cccccgagcg ccaggccgcc ctggacgagg
      781 tcgtccacac ggtgctgccc ggccgcccgg tccgcaacct caccaccgcc ggaccgccgc
      841 ccgccggccc gagggtggag cggctgccgt gatcaggatc gccgcggtcg gcgacgtcca
      901 cgccggggtc gactcgaccg gccggctccg ccccggcttc gagggcctcg gcgaccaggc
      961 cgacctgctg ctgctcgccg gcgacctcac ccaccgcggc cgggccgagg aggccaaggt
     1021 tctggccgac gagctggacg ggctcggcgt gccggtcgtc gccgtgctcg gcaaccacga
     1081 ctaccactcc gaccagcagg acgaggtcgc ccaggtgctc gccgacagcg gcacccaggt
     1141 gctggagggc gacgcgatca ccatccaggt cgccggccgc cggctcggca tcgccgggac
     1201 cagggggttc ggcggcgggt tcgccggcgc ctgcgcgagc gacttcggcg agccggagat
     1261 gaagaccttc acccgtctca ccaagcgcct cgcggcccag ctggagggag cgctcgcggg
     1321 cctgcgcgac accagcgact tccgcgtggt gctgctccac tacgccccgg tcgccgccac
     1381 tctggccggc gagccctgcg agatccatgc cttcctcggc agctacctgc tcgctgaggc
     1441 ggtcgaccgc gccggcgccg acctggtcct gcatggccac gcccaccgtg gcagccgcgc
     1501 cggcaccacg cccgggggcg tgccggtccg caacgtcgcg cagccggtga tcgggcgtgc
     1561 ctatgaggtc ttctgcctgg acgtggacac ggcaccgtga gcaacccgag caccgcaaac
     1621 cttaggaaag tctcatgaaa gacttctagg cgcatcccgg tcatcacggt acgctacggg
     1681 cacgcatttt gggacggccg gatgccagga ggagcccatg cccaccgccg gtgctcgcgc
     1741 tcgaacccga atccgacgac tgcacccccg cgctcgatct cggcctcggc ctctgctggg
     1801 ggcgttggca gccgcgagca gcctggtgct gttggcggcc ctgcccagcg gcgccagcgc
     1861 gggccccgcc gcctccacca cgaaggtgaa cgtcgcccga cagcacgccg agctggcacg
     1921 gcaggcgttc gtcgcgcgca cggccgcccg cgcccacccg cacgcggtgg cgccctcccc
     1981 gcaggcgtcc ccgctcgccg gcaccgccgc cggggccgcc ttcagcacca tccgcgacgt
     2041 cgagcagatc agccgcgaca ccctcccgtc gatccgcggc gccgagcccg acacccagac
     2101 agagcccgac gtcgccatcg acccgcgcaa ccccaaggtc atcctcaccg gcttccagca
     2161 gggccgctac aacgacggcg gctcggtcga cccggggttc gcgacctcgc aggacggcgg
     2221 tcgcacctgg gcggacggca acctgccgaa gctcaccatc gcggtcgggg gagcgtggga
     2281 gcgcgcctcc gacatcgcgg cggccttcgg cccggacggg tcggcctatc tgcagacgat
     2341 cccgttcaac gtgaccaact gccgcagcgg gatcgccatc cagcgctcca ccaacggcgg
     2401 gctcagcttc ggccggccgg tgctcaccgt cgacgacaac gactgcaaca tcttcaacga
     2461 caagaactgg ctcaccgtcg acaccttccc cgccagcccg cacttcggcc ggatctacgc
     2521 ggtctggtcg cggttcatcg ccaccagccc caccaccacc gtctcgcccg cgacgatcag
     2581 ctggtcggac gacaagggcc ggacctggtc gccgttccgc ttcatctcag cagccactga
     2641 cagcaccgag ggcgtcctgc cactcgtgcg gccggacggg tcgctcgcgg tggtctacga
     2701 ccagaccatc ggcactcagg acttcgaggt ctcccagacc tcccacgacg gcggcgcgac
     2761 ctggggcccg caggtccgga tcggccagtt cctcggcgcg ttcgtcccca acacccgcag
     2821 cggcggcctt cccaccgcca ccgtcgaccc ggtcaccggc gacctctacg ccgcctggga
     2881 ggacgcccgc ttcaaccccg ccggactcaa cgacatcgtc ctgtcccgct ccggggacgg
     2941 cggcgccacc tggagcccgc cccaggccgt caccccgcgg caggccggcg ctgaccgctt
     3001 caccccggac gtcgccgccg cgggcggcgc cgtccacctg acctaccgga cccgcggagc
     3061 gggcggccac gctgccacgg tcaccgagga ctacctcgcc tccagcgacc ggggcgtcac
     3121 cttcggcgcc cagcacgtgg ttggcccgcc aagcgcggtc cgctgggccg cccatgcctc
     3181 cggcctcctc ttctggggcg actacatggg cgtcgccgcc acccccacca aggccgccct
     3241 ggtctggtgc gtctcctcca acccacccgt cactggcgag gccttccacc agacaacctg
     3301 gggggcggtg gtgctccggt aggggttggc gaaccgcggc tcgggcgctc cggctccggc
     3361 gagatcgtca ggccgtgtgg gcccagatga ggcagtcgtg ccaggtgtcc tgtgctggat
     3421 catcatgctg ccaggcgcgg cagtggcgcg gcaggcgctg ctgcgggtgg tagccgcccg
     3481 gcgtgcgacc tgcagcgacg ccaggttgtc aggatcgatc tccagccaga cccgggaaag
     3541 gcctgccgtg gtgtgcgccc acagcgtcag ggcctggacg ctccgcacag cgatgccgcg
     3601 acggcgctgc tctgggcgca cccagtaggc cagctcggcg ctgctgctgt gggggctgtc
     3661 cggcatgagg ccgagcgcgg cgaccagccg cgggccatcc agcactccga aggagacctg
     3721 ccgcgcctga tcggggtccc aggcccgcaa ggccgaccac gcagcttcca gcgagtaggg
     3781 gccgacgggg cggggccccc atagggcagg cgctgtctct tcccgggtgg cctccgccag
     3841 caacgccgca tcctcatccc gcacactcag ggtgcgcatg cgcagcccgc ccaggtcgag
     3901 atcgacgagt cgttccatgt ccgccccagt gatcgtcgac tgctcggcgc cactctacgc
     3961 ggcacgatcg atctggcctc gcctacgagc taacccaagc gcaccagcgg ctgagcgcca
     4021 tgctgctgtt ggcgtacggc ttggtcatgg ccgcacactg tacggatcgc gcgcctggca
     4081 ggactcgaac ctgcgaccaa ctgcttagaa ggcagctgct ctatccggct gagctacagg
     4141 cgccggtgtg ttgcttggct ctaacgctac aaccacaacg acatccggcg cgggctgccc
     4201 gcgccggatg tccggatgtc cggatgtcga ggcggttata ccccgatggg tcgcaggacc
     4261 ctggtgtcgg cggtggtggt gctggcggcg ttggtgcgct gcttcatcag ttcgaagccc
     4321 tcggcgcggg tggcgacgct ggaggcggcg agccagcgga gccacggggc ggagacgaag
     4381 gcggactccg tcttgccgtc ggggtgctgg atgcgcagcc acatgcccgt ctcagtcagc
     4441 tcgacgccga cgccgtaggc ggggaccgga tccgcgaggg ggctgaagcg atgcagttcc
     4501 ggggtggctt ccgggcgccg ggtgaagttg agcatgaggc tgctcctttc ctggacttcc
     4561 cgcgccgtca acgcttgacc actgacggta cggctgagct gtccgtatag tgcgacttca
     4621 gcgccaccaa ggctgtcggg agcccgactg tcttcccgtc gagtagctgc cgcagttcac
     4681 gtcaccaagt tgaccaggcg gccaggaacg aacaccaccc ccgcgggacg ccggtggtgc
     4741 gtcctcatgc tccaagtatg ccagatggga tgaagcgaat atcgcggcca cgcgagctgg
     4801 catactcggt gcatggcgga cggagcagcg ggtactcgcc cgaaggtgcg tctcggcgtg
     4861 atctacgccg ctcatggtgc caaccacgcc ttcgtgctcg tcctgccggc ggtgctggtc
     4921 agcctccggg ccgagttcgg ggcctcgttc accaccctcg gcacggtcgc caccgtcagc
     4981 acgatcctct acgggctcgg ggcgctcccg gccgggctgc tcgccgaccg gttcgggccg
     5041 ccgcagctgc tgcggctgtt cgccgcgctg tcgatcgtgt gctgtgtgct cgcggcgctc
     5101 gccccgggca tctggtggct cgcggcggca ctggccctgc tcggcgcggc gggcgccttc
     5161 taccacccgg ccgggcttgc cgagctgacc ctcaccgcgc caggccaggg ccgcgtgctc
     5221 ggcctccacg gcagcgccgg caacctcggc accgcgctcg cgccgctgct ggctggcgcg
     5281 gtcgcctcgg cgtggacctg gcggggcagc tacgcccttg ccgggatcgt cgccgccgtg
     5341 ctgctgctgg cggtgcgccg gggcgtgccg ctggaccggc agctgccgcc agcggaccag
     5401 gctgggccga gggagcgcaa ccgcccggtg ctgtggctgg tgatgttcct cgcggtcgcc
     5461 gagggcttca tctaccaggg gttcgtggtc ttcctgccgg cgttcctggc cgcggtcggc
     5521 cacttcaacc gggccgccgc cgccaagggc ggggcgctct ccgcgctggt gctgctcctc
     5581 ggcgtgcccg gccagtattt cggtggccgc atcggcgagg ccgggccccg tggcctggcg
     5641 gtgcgctacg ccgccctcta cggcggcgcc gccgtcgtcg ggcaggccgt ccactgggcc
     5701 ggcgcgagcc tgctcggggt ggtgctggcc agcgtgttca gtctgctgat cttcctcggc
     5761 cagcccctca ccaaccagct cgtcgcccgc ggcaccagcg ccggacgccg cggcgccgcc
     5821 tacggcgggt acttctcgct ctcgttcgga gtcggcgcct tcgccggtac cgctggcggg
     5881 gtggtcgcgg accacgcggg gctggccgcg gtgttcgccc tcctcgggct ggtcgccctc
     5941 gtcaacgtct gcggcgggct caccgtccgt gcgctgctcg cccgcgaagc ggtgtagggt
     6001 cagccctgcc caaacccaga gccgaaaggg agaggcagcc atggctaccg ccgaccgccg
     6061 ggcccatgcc ctgtgggaag gatcgctgac cgagggcaag ggcgacgtcg agttcgccag
     6121 tagcggcatc ggccgctacc cgatcacctg ggcgtcccgg gtcgagcgcc ccgacggccg
     6181 caccagcccc gaggagctgc tcgccgcgag ccatgcggcc tgctacgcga tggccttctc
     6241 ctatgcgctc ggcggcgccg ggcattcgcc cgagcgcctc cacgtgaccg ccacggtcac
     6301 cctcgacccc aaggagggtg gcggcttcca cgtcacccga tccgccctgg aggtccaggg
     6361 ccgggttccg ggcctcgatc aggctgggtt ccagcagctc gccgagcagg ccgagcaggg
     6421 ctgccccatc tccaacgccc tccgcggcaa cctggccatc accgtgaccg ccacgctgga
     6481 gtcctgagct cggccagcct cctatcgagc ggccgccgtg gtcgccctgg ccccaggcca
     6541 ggggccaggc ggccgctcgg ccagctcctg ctcggccgtc tcgaccccgc agacctcgaa
     6601 gccacgagct tcgtagttgc gcttggcctg cggcccgtcg agcgagcagg tgtgcaccca
     6661 cacccgtgac ggcccgtgcg cccaggcgcg ttccaccccg gcgctgagca gctgcccacc
     6721 aaagcctcga ccaacgaagc gcggcagcag cccgaagtag gcaagctcga tctgcggccc
     6781 cggttggcgt tctagctcca ggtagccgac cggcgtgccg cgccagcagc caagccacag
     6841 ctcgtactcg tcgcgtccca cccaccgctg ccagtcggca taggaccagc cgagccggtc
     6901 ggtccagtac cagtcgccac ccaccgcggt atagaagaat cgactcagct ccggcaccgg
     6961 ctcccacgcc cgctcgaccg ccagcccctc gacccgccgt gctggccgca gttgctcggg
     7021 ccgacgcatc tccagcgtcc aggtcgtcac cgatcgccgc acacccatgc cgctccttcc
     7081 atccacgccg tcgtgcgacc aggatgccag gcggccaagc gaggccgagc gatctcagca
     7141 gcggagccct ggagcgggtg acgggaatcg aacccgcgtc acaagcttgg aaggcttgag
     7201 ctctaccatt gagctacacc cgctgaccag gggagcgtac cacctgcccc gctacgactc
     7261 gtcggggacg cgaagagcgg tgaacgcccc gccctgcggg tcacgcaggt tcgtgaagcg
     7321 cccgtatggc gagtccatcg gcggggagtt cacgccgccg ccgagccgct gcgcggtgga
     7381 ggcggtcgcg tcggtgtcct cgaccacgaa gtacaccgtc cagtgggacg gggtgtcggc
     7441 ggggaactgc tcgttcatct gcaacgcgcc gccgaccatc cggcctccga ccaggaaggt
     7501 cgcgtagcgt gggcctccgc ccgtgtcgac gtcctcccag tcgtagccga ataccgccgt
     7561 gtagaaggca cgtgccgcgt ccaggtcacg ggtcgccagc tcattccaga tcatcgcccc
     7621 cggctcgttg accagcgacg ccccaatgtg gctgcgggcc tgccacaggc cgaacacggc
     7681 cccggtcgga tcagcggcga tcgccatgcg cccctggtcc agcacgtcca tgggctccat
     7741 gttgagccgt ccgccagcat cgtggacccg cttggccgcc ccgtccgcgt catcggtcgc
     7801 gatgtaggtc gtccaagccg tcgtcatccc ctgcggcgcc gtctccccac caatgccagc
     7861 gacgttctgc ccatggagct tgcagatcgc gtagtgcccc gtctccgctg ggccgatgtc
     7921 gtactcccag ccgaacagct gaccatagaa accacgcgcc ccctccgggt cgctggtcat
     7981 caggtcaacc catgagggcg tccccgcggc atacgacgtg acctgagcca tgctccacct
     8041 ccgttggtcg gaatcgaata cctgttcgaa cagtgggagc ataacccacc tccccaacag
     8101 gtacgagtct gctctactga gccagggcag tgctgccgag tcaggtagag agtctgcgcc
     8161 cagttgaaac catgaggacg acgcgggagg accatcatgt gggagtacga gcacgccgtg
     8221 accaccagcg ccgacaccca ggcgctgtgg cgccactggt cggacatggc ggcatggccg
     8281 acgtggaacg aaggtattga gaagatcgag atcgatggcc cgttcgccgt gggtac
//
LOCUS       DASWDC010000139         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_866585, whole genome shotgun sequence.
ACCESSION   DASWDC010000139 DASWDC010000000
VERSION     DASWDC010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866585"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..581
                     /locus_tag="VF995_04470"
     CDS             252..581
                     /locus_tag="VF995_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX9342852.1"
                     /translation="MRSLELFPPGSALPCVQAGPDLYFSDSPRDLEVAKRLCRPCPLR
                     SACLAGAVERHEPWGVWGGEVFVDGRPVPFKRGRGRPRKHPRPLDLDLDLDLDLEPVD
                     LESAKAS"
     gene            614..787
                     /locus_tag="VF995_04475"
     CDS             614..787
                     /locus_tag="VF995_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342853.1"
                     /translation="MHQNLGEYLNRSQELAREADSHHSRHLRALRAKRAAELDEALAR
                     REWSALLRALTRR"
     gene            996..1715
                     /locus_tag="VF995_04480"
     CDS             996..1715
                     /locus_tag="VF995_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342854.1"
                     /translation="MPSLDDGLLAQLDILIDLAADRGGLLLDRDERLARSETSLLASA
                     LGLDPIPAGPRSSAPAVEAPPRFGDDAVELLRSLAESVGILREHAGRLEVTSLYGAWR
                     RIALQLRAGLVYASWCHRVHWPNLVANGDDLAAEQLAHRRIRVLRLLFDLPAGVAVTL
                     TGLADTVADATGLPAGPWLCSALTAVFLDPLVALGVAQLEPDSLRPPTTLHLGPLART
                     VIGSALVAAGEEVPLAPAGAN"
     gene            complement(1743..>2411)
                     /locus_tag="VF995_04485"
     CDS             complement(1743..>2411)
                     /locus_tag="VF995_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9342855.1"
                     /translation="RLTRGVLEVLRDFANPCSILTKSPLLLRDLDLFEELVGVGFSAS
                     LSIGTLDEEVWRRSEPGTPHPRARIAAVKALVEAGVPCGVLVAPVLPGISDAPAQLRA
                     VVDAAAAAGATHISPITLHLRRGVKEEFMPWLEREYPELVDGYRRMYRGANAPELVQR
                     RIAERVRAAKATARAGDAVAPVAAAANQRTDHRGIGSAPPPEPEPEPEPEPEAPAQLS
                     LRLG"
BASE COUNT          375 a          994 c          722 g          320 t
ORIGIN      
        1 cgacgggacc cgtgtgccta cgaccaagtt cggttggacc cagcagaacg acctggcctt
       61 cgcggccttg taccaccggg aggaaccgag atagggacct ccccggagcg gacaaaagtc
      121 cctccggaac accgcgaagg ccgtgggcac cgcccacggc ctttctgttt ggcaccaacc
      181 aggaccgact gacgactgac gaccgacgca gacaaccgac gagacgcgac caacggagga
      241 gcgaccagac catgcgatcg ctggagctgt tcccgcccgg atcagcctta ccgtgcgtcc
      301 aggcaggacc cgacctgtac ttctccgact cccctcggga cctggaggtg gccaagcgac
      361 tatgccggcc ctgcccactg cgcagcgcct gcctggccgg cgccgtcgaa cgccacgaac
      421 cctggggcgt ctggggcggc gaggtgttcg tcgacggccg gcccgtgccg ttcaagcgcg
      481 gccggggccg gccccgcaag cacccgcgcc ccctcgacct ggacctggac ctcgacctcg
      541 acctggagcc ggtcgacctg gaatcggcca aggccagttg acccgacccg cgcctgacag
      601 gaggtgaatc tcgatgcacc agaatctcgg tgaatacctc aaccgcagcc aggagctggc
      661 ccgcgaggcc gacagccatc actcccggca tctgcgcgcc ctgcgcgcca agcgcgccgc
      721 cgaactcgac gaggcactcg cccgccgcga gtggtcagcc ctgctcaggg cgctgactcg
      781 ccgatgagcg gcccggcgac cccgactccc agagcccgtg ctcagcacgg ctctgggagt
      841 cgattcgtgc ccaggcccag accacgggca cgcgcgggag aagttatcca caacttggtg
      901 tttacctctg tttccttcgc ttagcttctg aaaagctgtg actcccccaa agttccaccg
      961 tcacagcaac gtaggaagaa gcgaagggac acaccatgcc aagccttgat gacgggctcc
     1021 ttgcccagct cgacatcctg atcgaccttg ccgccgaccg cggcggcctc ctcctcgacc
     1081 gcgacgagcg gctcgcccgc agcgaaacca gcctgctcgc cagcgccctc ggcctcgacc
     1141 ccatccccgc cgggcccagg tccagcgccc ccgccgtcga agccccaccc cgattcggcg
     1201 acgacgccgt cgagttgctc cgcagcctcg ccgagtccgt cggcatcctg cgggaacacg
     1261 ccggccgcct cgaagtcacc agcctttacg gcgcctggcg gcgcatcgcc ctccagctcc
     1321 gcgccggcct cgtctacgcg tcctggtgcc accgcgtcca ctggccgaac ctcgtcgcaa
     1381 acggtgacga cctggccgcc gagcagctcg cgcaccgccg gatccgggtc ctgcgcctgc
     1441 tgttcgacct cccagccggg gtcgcggtca cgctcaccgg cctggccgac accgtcgccg
     1501 acgccaccgg gctgcccgcc ggcccctggc tgtgcagcgc cctgaccgcc gtcttcctcg
     1561 accccctggt cgcccttggc gtcgcccagc tcgaaccgga ttccctccgg ccacccacca
     1621 ccctccacct cggccccctg gcccgcaccg tgatcggctc ggcactggtc gccgccggcg
     1681 aggaggtgcc cctggcaccg gccggagcga actgagcacc tccggtggcc tccccaagtg
     1741 gcctacccga gccgcagcga cagctgcgcg ggcgcctccg gctcgggctc cggctcgggc
     1801 tccggctcgg gtgggggagc ggaaccgatc ccccgatggt ccgtccgctg gttcgccgcc
     1861 gcggccaccg gcgcgacggc gtcgccggcc ctcgccgtgg ccttggccgc gcggacccgc
     1921 tcggcgatcc tccgctggac cagctcaggc gcgttcgcgc cccgatacat gcgccggtag
     1981 ccgtcaacca gctccggata ctcgcgctcc agccacggca tgaactcctc cttcacgccc
     2041 cgccgcagat gcagcgtgat cggggagata tgggtcgcgc cggccgccgc cgccgcatca
     2101 accaccgccc gcaactgcgc gggcgcgtcc gagatgcccg gcagcaccgg cgcgaccagc
     2161 accccgcacg gcaccccggc ctccaccagc gccttcaccg ccgcgatccg cgccctcggg
     2221 tgcggcgtcc ccggctcgct ccgccgccac acctcctcat ccagcgtccc gatcgacaag
     2281 ctggcgctga accccacccc aaccagctcc tcgaacagat ccagatcccg cagcagcaac
     2341 ggcgacttcg tcaggatcga gcacggattg gcgaagtccc gcaacacctc caacaccccc
     2401 cgcgtcagcc g
//
LOCUS       DASWDC010000140         8610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_601907, whole genome shotgun sequence.
ACCESSION   DASWDC010000140 DASWDC010000000
VERSION     DASWDC010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8610
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601907"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /gene="dinB"
                     /locus_tag="VF995_04490"
     CDS             complement(<1..873)
                     /gene="dinB"
                     /locus_tag="VF995_04490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX9342856.1"
                     /translation="MTANGPQPRPAGKRVERLTPTGEVVAGDLPLDALDTKDLDGLGI
                     VHADLDAFYANVEVLKDPGLRGRPVLVGGTGNRGVVSSASYEARAFGCRNAMPMAHAR
                     RLCPQAVVLPPDFASCRRYSRQVLGIFQAVTPLVEPLALDEAFLDVRGARKLFGDAVQ
                     IARELRRRVREETGLTLTVGVAANKYLAKLASTRGKPDGLFVVPPSRALEFLHPLPAE
                     ALWGAGSATIAALGRYGLRTVGEVANAPRALLERALGQAAGAQLAELAWGRDERPVEP
                     YEAAKSVGSEETFAA"
     gene            921..1655
                     /locus_tag="VF995_04495"
     CDS             921..1655
                     /locus_tag="VF995_04495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018730381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9342857.1"
                     /translation="MSHRRVLVPLLLGALIAAAAGCSGGRARPAAASGSGGSGTSASA
                     TTAATPSTASAATTGTTAPPSTTGAAHYVFPVQGCASNYGHFHHDYPATDIFTSKGCR
                     FVAVTDGVVSEVNRIDRWDPKTNLGADRGGRSVAIVGVDGVRYYGSHLLSVATGIEPG
                     VHLHAGQLLGLVDNSGDARFTATHVHFGISWPTGPGIWWIRRGVLYPWPYLDSWRAGG
                     SRSPATAVKAALAAAGTPTPPCRAEC"
     gene            complement(1688..3052)
                     /locus_tag="VF995_04500"
     CDS             complement(1688..3052)
                     /locus_tag="VF995_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342858.1"
                     /translation="MRDDELRRLFQMFGDPVADAVNPPTPAQIRHRGQRRRRRVALAS
                     TMAVLIVAAAGVGVVQARNLSRTSPTHPGPEPAVTSASVPLTTSPPAPPTSGATGQAT
                     TATTLPLGTVGELHAIQFLSATQGWAAGRGAILVTSDGGQHWGRQYGGNANLLTIDFF
                     DARHGWAVATDPAPTLLGTSDAGQHWRALGEPSGGTLGKVHFDTASQGFGVAARNSSP
                     RDTRLVATGDGGATWRWLPTPSGAGAGTVVSDVCFSHPGDGWLVTSSSTGAGGTVWRS
                     AGASSGLTIAWHRSYQLNTGGDPVTGAQLQCAGPGSAWVQVAGGGAAGHIAYVLLNTR
                     NGGTTWKAVLVNQFTWRLLFPGVPDGPGSYPGPFSAVSPTAAFALGSTPPAGDLLSGM
                     LATAGATPGPARKVPALSAMRTPQGVSFVSATAGWVAGTDSTGHGVILATTDGGQSWK
                     AQLS"
     gene            complement(3045..3563)
                     /locus_tag="VF995_04505"
     CDS             complement(3045..3563)
                     /locus_tag="VF995_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013002129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEX9342859.1"
                     /translation="MADDGEFDHFYLRTYGRVVAELYGVVGSVQEAEDAVQEAFARAS
                     LRWQRLRDYDHPDAWVRRVALNLATSSLRRTSRKLAAYRRVGPPAPVPPPSEERVMLH
                     QALGRLRRHQREVLVLHYLADLSVEEIARVLQIPTGTVKGRLFRARAALERELGGSGE
                     EPVPGGNEVRHA"
     gene            complement(3635..4900)
                     /locus_tag="VF995_04510"
     CDS             complement(3635..4900)
                     /locus_tag="VF995_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEX9342860.1"
                     /translation="MADLSIRLRDASPGLLALPWLRPLGEWTVEQATLRELPVGPSRH
                     LVRFAEADGVLYALKELPTRVAAKEYRVLRTLEDRELPAVRPVGLVEQPAGGNSILVT
                     EYLGHAWQYRRLFQRLSRTLARHRERLLDAMAGLLVDLHRAGVFWGDCSLANTLFKRD
                     GQALQAFLVDAETSEMHPSLSRGQRELDLGILVENVTGDLLDLALAMGETGEHVDADL
                     AAAESVATRYWQLWDELTLEQTFRVDERYKVAARVRRLNELGFAVDELALEPVEHDRS
                     RLRLKVAVANRRFHSEELRRLTGLEVGEGQATVLLNDLRGYQCRLEHERPGSAGDEVI
                     GYRWLAECFRPGVTKAALVLDPASDPIQAYCDLLEVRWLLSERSGHDVGDEAALEALA
                     EHRPPAESVAEMAVVEATTSNFAVVDQDG"
     gene            4973..6859
                     /gene="dxs"
                     /locus_tag="VF995_04515"
     CDS             4973..6859
                     /gene="dxs"
                     /locus_tag="VF995_04515"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX9342861.1"
                     /translation="MLESIREPADLRGLSGKQLTQLAAEIREFLVNAIAVRGGHLGPN
                     LGVVELTIALHRAFDSPTDQIVWDTGHQAYVHKLLTGRQADFATLRTKDGLSGYPSRS
                     ESAHDVIENSHASTALGYALGLAETRRAGRGEGRVVAVVGDGSLTGGVALEALNLIGH
                     RKPDLLVVLNDNGRSYQPTVGGLAGHLAPLRLHPGYEQLKKGVEETLGRFPMVGDGMV
                     EAAKRVKEGAKALVAPRVVFEDLGWQYAGPVDGHDIAALERVLDHAMRVPGPVIVHAI
                     TQKGRGYPPSEQHEEERHHSVPAFDPATGKAITKPSDEVPFTSIFGEALLEEARRHPE
                     LVAISAAMLSPTKLTIMEREFPERVYDVGIAEQLAVTMAAGMALQGLRPVCAIYSTFL
                     QRAFDQVVMDVSLHKLPVVFAIDRAGITGDDGPSHHGVFDLSYLRQIPNMVVAAPRDG
                     TELRRVLATATAHTTGPFAIRWARGTTPKVDFTQPVRKLKVGNWEVRSRGSDVLLLGL
                     GALHGACEEAARQLKQDGISVTLVDPRWVKPLDPRLASVAGGHRLVVTVEDNVLAGGF
                     GAAVAEVLADEEVPTPLLRLGIPDRFLVHGKRDLLLTELGLDAVGIAERIRKRLHHLD
                     HNAG"
     gene            complement(6885..7220)
                     /locus_tag="VF995_04520"
     CDS             complement(6885..7220)
                     /locus_tag="VF995_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342862.1"
                     /translation="MAFDDELKLLGFQFNQERRDGTRTWSLFRNRFLTYWLHLPLEEE
                     PLALFTWEFALGEYMDEYGLQVGANEPLNQFLFPQVDTEVEQNLAAVVAAMERTEAMF
                     RGMDFGHRG"
     gene            7424..>8610
                     /locus_tag="VF995_04525"
     CDS             7424..>8610
                     /locus_tag="VF995_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9342863.1"
                     /translation="MARHIAAPDSLVGRTIGRRYEIVAMVARGGMAIVYRAHDRQLDR
                     PVAVKVPRAEFARDRGFSEQFRREARAAARLSHPHMVAVYDSGEERGLPWIVMEYVPG
                     QTLREVLDARGRLDLAATAELLGPVADALDHAHRAGVVHLDVKPENILLTSDSVKVTD
                     FGLVRAARGDRAHAPAGTVQYCAPETLRGGMVDGRADVYALGVVAYECLTGRPPFTGT
                     SSEQLVRQVLNERVPPPSVWARDLPEEADAAVRRATDPDPASRWSASTEFTAAIGAPR
                     RRRVDETLRATTVLPAAAQAQAPPPSASASARETVAAMPLTAPPLRRGRQRPLRPRSG
                     RLPKPPGRPKRQWRRSSVLLAFTLAALLAVGVAGGWLVAGPTRRVPSLVGMGFDHATA
                     SLRA"
BASE COUNT         1171 a         3182 c         3094 g         1163 t
ORIGIN      
        1 ggcggcgaag gtctcctccg agccgaccga cttggccgcc tcgtagggtt ccaccggccg
       61 ctcgtcgcga ccccacgcca gctcggcgag ctgcgccccg gcagcctggc cgagggcgcg
      121 ctcaagcagg gcgcggggcg cgttggcgac ctcaccgacc gtccgcagcc cgtagcggcc
      181 gagcgcggcg atggtggcgc tgccggcgcc ccagagcgcc tcggcgggca gcgggtggag
      241 gaactcgagc gcccggctgg gcgggaccac gaacagtccg tccggcttgc cgcgggtgga
      301 ggcgagcttg gcgaggtact tgttggcggc gaccccgacg gtgagggtga ggccggtctc
      361 ctcccggacg cgacggcgca gctcgcgggc gatctgtacg gcgtcgccga agagcttcct
      421 cgcgccgcgc acgtcgagga acgcctcgtc cagggcgagc ggctcgacca gcggggtgac
      481 cgcctggaag atgcccagga cctgcctgga atagcgccgg cagctggcga agtccggcgg
      541 gagcacgacc gcctgcgggc acaggcgcct ggcgtgcgcc atcggcatgg cgttgcggca
      601 cccgaacgcc cgcgcctcgt aggaggcgga gctgaccacc ccgcggttgc cggtcccgcc
      661 gaccagcacc ggccggcccc gcaggccagg gtccttgagg acctcgacat tggcgtagaa
      721 ggcgtccagg tcggcgtgca cgatgcccag cccgtcgagg tccttcgtgt cgagggcgtc
      781 cagcggcagg tcgccggcca ccacctcgcc ggtcggcgtc agccgctcga cccgtttgcc
      841 ggcgggccga ggctggggac cgttcgcagt catgtgccgt aggatctcgc ggtagcccga
      901 caacgagaag cgagttcgcc gtgtcccacc gccgagtcct cgtcccgctg ctcctcgggg
      961 ccctgatcgc cgcggccgct ggctgttccg gaggccgcgc ccgcccagcg gccgcatccg
     1021 gcagcggcgg gagcggcacg agcgccagcg ccaccaccgc cgccacgccc tccacagcat
     1081 ccgccgccac cactggcacg accgcgccgc cgagcaccac cggggcggcc cactacgtgt
     1141 tcccggtgca gggttgtgcc tccaactacg ggcacttcca ccacgactat ccggccaccg
     1201 acatcttcac ctccaagggc tgccgcttcg tcgcggtgac cgacggggtg gtcagcgagg
     1261 tgaaccggat cgaccgctgg gacccgaaga ccaacctcgg cgccgaccgg ggcggccggt
     1321 cggtcgcgat cgtcggcgtc gacggcgtcc gctactacgg ttcccacctg ctgtcggtcg
     1381 ccaccgggat cgaaccgggc gtgcacctcc acgccgggca gctcctcggc ctggtcgaca
     1441 actccggcga cgcccgcttc accgccaccc acgtccattt cggcatctcc tggccgaccg
     1501 gcccggggat ctggtggatc cgccgggggg tcctgtaccc gtggccgtac ctggactcct
     1561 ggcgtgccgg ggggagccgc tcccccgcca ccgccgtcaa ggctgccctg gccgccgccg
     1621 ggaccccgac cccgccctgc cgggccgagt gctgatgccc gacctcaccg cccgcctcgg
     1681 ccggccgcta gctgagctgc gccttccagc tctggccgcc gtcggtggtg gccaggatga
     1741 cgccgtgccc cgtgctgtcg gtgccggcca cccagcccgc tgtcgcgctg acgaagctga
     1801 ccccctgcgg ggtgcgcatg gcggacaggg cggggacctt ccgggccggg ccgggggtgg
     1861 cgcccgcggt cgccagcatg cccgacagca ggtcgccggc tggcggagtc gagccgagcg
     1921 cgaacgcggc cgtcgggctg accgccgaga acggcccggg gtagctgccg ggcccgtccg
     1981 ggacgccggg gaacagcaag cgccaggtga actggttgac cagcaccgcc ttccaggtgg
     2041 tgccgccgtt gcgggtgttc agcagcacgt aggcgatgtg cccagcggcg ccgccgccag
     2101 cgacctgcac ccaggcggac ccggggccgg cgcattggag ctgcgcgccg gtgaccgggt
     2161 cgccgccggt gttgagctgg taggaacggt gccaggcgat ggtcaggccg gagctggcgc
     2221 cagcggagcg ccagacggtg ccaccggcac cggtggagct gctggtgacc agccagccgt
     2281 cgccaggatg gctgaagcag acgtcgctca cgacggtccc agcaccagcg ccggacgggg
     2341 tgggaagcca tctccaggtg gcgccgccgt cgccggtcgc aaccagccgg gtgtcccgcg
     2401 gcgagctgtt cctggcggcg acgccgaaac cctgcgacgc cgtgtcgaag tggaccttgc
     2461 cgagggtgcc gccggacggc tcgccgaggg ctcgccagtg ctggccggcg tcgctggtgc
     2521 cgagcagcgt cggcgccggg tcggtggcga ccgcccagcc gtggcgcgcg tcgaagaagt
     2581 cgatggtgag caggttcgcg ttgccgccgt actggcgtcc ccagtgctgg ccgccgtcgc
     2641 tggtgaccag gatcgccccc ctgccggctg cccagccctg ggtggcggag aggaactgga
     2701 tggcgtgcag ctcgccgacg gttccgagcg gcagggtcgt ggccgtcgtg gcctggccgg
     2761 tggcgccgct ggtgggcggg gctggtgggc tggtggtgag cgggaccgag gcggaggtga
     2821 ccgctggctc ggggcccgga tgcgtcggcg aggtccggct gaggttgcgg gcttgcacca
     2881 cccccacccc ggcggcggcg acgatcagca ccgccatcgt gctggcgagc gcgacccggc
     2941 ggcggcgccg ctggccccgg tggcggatct gggcgggggt gggcgggttg accgcgtcgg
     3001 cgaccgggtc gccgaacatc tggaacaggc ggcgcagttc gtcgtcacgc atggcgcacc
     3061 tcgttccctc cgggaaccgg ctcttcgccg ctgccgccga gctcgcgttc gagggcggcg
     3121 cgggcgcgga acaggcggcc cttgaccgtg ccggtgggga tctggagcac gcgggcgatc
     3181 tcctcgaccg agaggtcggc caggtagtgg aggaccagca cctcgcgctg gtggcggcgg
     3241 aggcggccga gcgcctggtg caacatcacc cgctcttcgc ttggcggcgg gaccggcgct
     3301 ggcgggccga cgcgccggta ggcggcgagc ttgcggctgg tgcgccgcag cgagctggtg
     3361 gccaggttca gggcgacccg ccgcacccag gcgtccgggt ggtcgtagtc gcgcagccgc
     3421 tgccagcgca gcgaggcgcg ggcgaaggct tcctgcacgg cgtcctcagc ctcctggacg
     3481 ctgccgacca cgccgtacag ctcggcgacc acgcgcccgt aagtccgcag gtagaagtga
     3541 tcgaactcgc cgtcgtcagc cacgcgcccc tccgtcgggt cactttgatc agccgagaca
     3601 cgccacagca cggcggttgg ttcgggtgac ctccttaccc gtcctggtcg acgaccgcga
     3661 agttcgaggt ggtcgcctcc accaccgcca tctccgcgac cgactccgcc ggtggccggt
     3721 gctcggcgag ggcctccagg gcggcctcgt cgccgacgtc gtggccgctg cgctccgaca
     3781 gcagccagcg gacttccagc aggtcgcagt aggcctggat cggatcggac gccgggtcga
     3841 gcaccagggc cgccttggtg acgcccgggc ggaagcactc agccagccag cggtagccga
     3901 tcacctcgtc gccggccgag ccgggccgct cgtgctccag gcggcactgg tagccccgca
     3961 ggtcgttgag cagcacggtc gcctggccct cccccacctc caggccggtg aggcggcgca
     4021 gctcctcgga atggaagcgg cggttggcga ccgcgacctt gagccgcagg cggctgcggt
     4081 cgtgctcgac cggctccagc gccagctcgt ccacggcgaa gcccagctcg ttgaggcggc
     4141 gcacccgcgc ggcgaccttg tagcgctcgt cgacgcggaa ggtctgctcc agggtcagct
     4201 cgtcccacag ctgccagtag cgggtcgcga cgctctcggc ggcggccagg tcggcatcga
     4261 cgtgctcgcc ggtctcgccc atcgccagcg ccagatcgag caggtcgccg gtcacattct
     4321 cgaccaggat gccgaggtcc agctcccgtt gcccacggct gagcgacggg tgcatctcgc
     4381 tggtctcggc gtcgaccagg aacgcctgca gcgcctgccc gtcgcgcttg aagagggtgt
     4441 tggccagcga gcagtccccc cagaacaccc ccgcccggtg caggtcgacc agcagccccg
     4501 ccatcgcgtc cagcagccgc tcgcggtgac gggcgagcgt ccgcgacagc cgctggaaca
     4561 gccgccggta ctgccaggcg tggcccaggt actcggtgac caggatcgag ttgccgccgg
     4621 cgggctgctc gaccagcccg accgggcgca ccgccggcag ctcgcggtcc tccagcgtcc
     4681 gcaacacccg gtactccttg gccgcgaccc gcgtcggcag ctccttcagc gcgtacagca
     4741 ccccgtcggc ctcggcgaag cggaccaggt gccggctcgg cccgaccggc agctcgcgca
     4801 gggtggcctg ctcgaccgtc cactcgccga gcggccgcag ccacggcaac gccagcagcc
     4861 caggtgaggc gtcacgcagc cggatggaaa gatcagccat ggaagcgatg gtaaaccgtt
     4921 accttccagg ggagcaggca tccagatcaa aaggcgctat cctcatccgg tgatgctcga
     4981 gtccatcaga gaaccggccg acctccgcgg cctctcgggc aagcagctga cccagctggc
     5041 tgccgagatc cgcgagttcc tcgtcaacgc catcgccgtt cgtggcggcc atcttggccc
     5101 gaacctcggc gtggtcgagt tgaccatcgc gctgcaccgc gccttcgact cgcccaccga
     5161 ccagatcgtc tgggacaccg gccaccaggc atacgtgcac aagctgctca ccgggcgcca
     5221 agcggacttc gcgaccctgc gcaccaagga cggcctctcc ggctacccgt cccgcagcga
     5281 gagcgcccac gacgtgatcg agaactccca cgcctccacc gcgctcggct acgccctcgg
     5341 cctggccgag acgcgccgcg ccggccgggg cgagggccgg gtcgtggcgg tggtcggcga
     5401 cggctccctc accggcgggg tggcgctgga ggccctcaac ctgatcggcc accgcaagcc
     5461 cgacctgctg gtggtcctca acgacaacgg ccgctcctac cagccgaccg tcggcgggct
     5521 cgccggccac ctcgcgccgc tgcggctgca tcccggctac gagcagctca agaaaggcgt
     5581 cgaggagacc ctcgggcgct tccccatggt tggcgacggc atggtcgagg ccgccaagcg
     5641 cgtcaaggag ggcgccaagg cgctggtggc gccccgggtg gtgttcgagg acctcggctg
     5701 gcagtacgcc ggcccggtcg acggccacga catcgccgcg ctggagcggg tcctcgacca
     5761 cgccatgcgc gtgcccggcc cggtgatcgt ccacgccatc acccagaagg gccgcggcta
     5821 ccctccctcc gagcagcacg aggaggagcg ccaccacagc gtgcccgcgt tcgacccggc
     5881 caccggcaag gcgatcacca agcccagcga cgaggtcccc ttcaccagca tcttcggcga
     5941 ggcactgctg gaggaggccc gccgccaccc cgagctggtg gccatctcgg cagcgatgct
     6001 gagccccacc aagctcacca tcatggagcg ggagttccct gagcgggtct acgacgtcgg
     6061 catcgccgag cagctcgcgg tcaccatggc ggcgggcatg gccttgcagg gcctgcggcc
     6121 ggtgtgcgcc atctactcga ccttcctgca gcgggccttc gaccaagtgg tgatggacgt
     6181 ctcgttgcac aagctgccgg tggtcttcgc gatcgaccgc gccggcatca ccggcgacga
     6241 cggcccctcc caccacggtg tcttcgacct gtcctacctg cgccagatcc ccaacatggt
     6301 ggtggcggcg ccgcgcgacg ggaccgagct gcgccgcgtg ctggccacgg cgacagcgca
     6361 caccaccggg ccgttcgcta tccgctgggc ccgggggacc acgccgaagg tcgacttcac
     6421 ccagccggtg cgcaagctca aggttggcaa ctgggaggtg cgctcgcgcg gctcggacgt
     6481 gctgctgctc ggcctcggcg ccttgcacgg cgcctgtgag gaggctgccc gccagctcaa
     6541 gcaggacggc atcagcgtca ccctggtcga ccctcgctgg gtcaagccgc tggacccgcg
     6601 cctggcgtcg gtggccggcg ggcaccggct ggtcgtcacc gtcgaggaca acgtgctggc
     6661 cggcggcttc ggcgccgcag tcgccgaggt cctcgccgac gaggaggtcc ccaccccgct
     6721 gctgcgcctc gggatccccg accggttcct ggtccacggc aaacgcgacc tgctgctcac
     6781 cgagttgggc ctggacgccg tcggcatcgc cgagcggatc cgcaagcgcc tgcaccacct
     6841 cgaccacaac gccggctagc gctcccggcg ggtcagccgg cgggtcagcc gcggtggccg
     6901 aagtccatac cgcggaacat cgcctcggtg cgttccatcg cggcgaccac cgcggcgagg
     6961 ttctgttcga cctcggtgtc gacctgcggg aacaggaact ggttgagggg ctcgttggcg
     7021 ccgacctgca ggccgtactc gtccatgtac tcgccgaggg cgaattccca ggtgaacagc
     7081 gccagtggtt cctcttccaa gggcaggtgc agccagtagg tgaggaagcg gttgcggaac
     7141 agcgaccagg tccgcgtgcc gtcgcggcgt tcctggttga actggaagcc gagcagcttg
     7201 agttcgtcgt cgaaggccat gggcgcattg tcggcgccgc cacccgcatc cgccaacgcg
     7261 ctggcaggat gcggcaagag atcaggcggt ctcaagctca tgagagttca tgtcagaatc
     7321 gagttaattc accgcttgac atgcctgggc ggtagaattt cgctggccgg ctgccgccgg
     7381 tgtcccagga ctacggtgca cggtcggtgc acggtgaagc ttcgtggcca gacatatcgc
     7441 cgctcctgac agcctcgtcg gccggaccat cggccggcgc tatgagatcg tcgccatggt
     7501 cgccaggggc ggcatggcga tcgtgtaccg cgcccacgac cggcagctcg accggccggt
     7561 cgcggtcaag gtcccccgag cggagttcgc gcgggaccgc ggcttctcgg agcagttccg
     7621 ccgcgaggcg cgggccgcgg cccggctgtc ccacccgcac atggtggcgg tctacgactc
     7681 cggcgaggag cgggggctgc cctggatcgt gatggagtac gtccccgggc agaccctgcg
     7741 ggaggtgctg gacgcgcgcg gccggctcga tctcgccgcc accgccgagc tgctcggccc
     7801 ggtcgccgac gcgctcgacc acgcccaccg cgccggggtc gtccacctcg acgtcaagcc
     7861 cgagaacatc ctgctcacca gcgactcggt gaaggtcacc gacttcgggc tggtgcgcgc
     7921 agcgcgcggt gaccgtgccc acgcacccgc cgggacggtg cagtactgcg ctcccgagac
     7981 gctgcggggc gggatggtcg acgggcgcgc ggacgtctat gccctcggcg tggtcgccta
     8041 tgagtgcctc accggccgtc cgccgttcac cggcaccagc agcgagcagc tcgtccggca
     8101 ggtcctcaac gagcgggtcc cgcccccgtc ggtgtgggcg cgggacctgc ccgaggaggc
     8161 cgacgccgcg gtccggcgag ccaccgaccc ggacccggcc agccgctgga gcgccagcac
     8221 cgagttcacc gccgcgatcg gcgcgccccg ccgccgccgc gtcgacgaga ccttacgggc
     8281 caccaccgtg ctgcccgccg ccgcccaggc gcaggcgccg ccgccctcgg cttcagcgtc
     8341 ggcgcgggag actgtggctg ccatgccgct gaccgctccg ccactgcggc gcggccgcca
     8401 gcgcccgctg cgcccgagaa gtggccggtt gcccaagccg ccggggcggc cgaagcggca
     8461 gtggcgccgc agcagcgtcc tgctcgcgtt cacgctggcg gcgctgctcg ccgtgggtgt
     8521 cgccgggggg tggctggtgg ccggcccgac caggcgggtg ccgtcgctgg tcggcatggg
     8581 cttcgaccac gcaacagcga gcctccgggc
//
LOCUS       DASWDC010000141         7953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_866805, whole genome shotgun sequence.
ACCESSION   DASWDC010000141 DASWDC010000000
VERSION     DASWDC010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866805"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VF995_04530"
     CDS             complement(<1..1340)
                     /locus_tag="VF995_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342864.1"
                     /translation="MPRHVVLLSRDSSLAVALRMLLGEADVVTEIDRPAQWPSAAELP
                     VSTAVVDLPAGWRLPGVEQVRARFRGPLVVLLGQEEDAGVIPAEHACTVMRRPFEIMT
                     LWSLVTGASASASASAAPGTGTAVPWREAPVGPAPKAAKPPLAPPGTRASAPPPSASP
                     SAPVVPPVAPGAPASPYPAPAPAPPVPPPARKTERKTERKAERKAERKTEREPEGPPS
                     QVPAVPPPPPPAPEAPAAAVGKPGGDPEPAWSGRLRRLHDVPAAPSPGSPAPPHQPPP
                     PPPRHEPAAGGGRRQAPAPPAPAPAAPAAPPPSQRQVAAAGAEQPLDLVAVAPEDASP
                     LPGLPVKERFRRLRDLPNTPSAGVRPGAGSGPIATAPLPDHQPAPAPGTGAASGSGER
                     AEHPEEAAPARAPAASPASAQPPAAPPVATPRRGATRPSRSQPVVPPPAAPTPAP"
     gene            complement(1477..2703)
                     /locus_tag="VF995_04535"
     CDS             complement(1477..2703)
                     /locus_tag="VF995_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX9342865.1"
                     /translation="MALLIVDPDDQSATWLAGILAELAEQLIRTTSPDEAMAAFATEG
                     PDVLGVVVGPGSSDADAVALAGRLQEAAPDVAVVLLRQEGQDSGELLRAALRAGVRDV
                     VTALSDPAAVRGACARALELTRALRGLGGAPDRRPAAAADPARAKAGKLITVFSSKGG
                     CGKTFVATNLALALAAHAEVALVDLDLHFGDVAIMLQLFPARTIQDAARASGGGLDPV
                     TMKSFLTRHQSGLWALVAPSEPTVADTISPTAVVTILRALRQRFGYVVVDTPAAFSDQ
                     VLAAFDESDAIAMLATLDVPSIKNLKLALRTMESLRYPRDKTCLVINRADSKVGLRLP
                     DVERILDTQIDVTLPSSRAVPLSINKGSPILVEEPKSGVAEAIRRLADQLATAAPGRP
                     ARAGRGRSRRSLFQRS"
     gene            complement(2862..4073)
                     /locus_tag="VF995_04540"
     CDS             complement(2862..4073)
                     /locus_tag="VF995_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX9342866.1"
                     /translation="MSVPVLVLDSADGIGLWMRDALLDAGLACTLAREDDEAGRSLDA
                     QRPALVAVQVEQVTVWTPVLQRLGEMTPRVPVLGVVGRDAPIDFTPALALGVTDLLTY
                     PCSAEDLVIRVASSMRGRHDPHRVPERVEPVSPLATLRSVTEAAEEALLRTETAAARV
                     NRSEEREHLRSARESLAHSLQVILGTMIGTAEASGHGREGHARLAAILTREMTVELGW
                     SPMRVRGMELAAMFYDIGLLDVPGNTLAVPGALDPAAVELLRRHPDVAADILEPLSRV
                     GVPVAAVRAHHERLDGSGYPRGLRGRQIPFGAQLLAVVDTYAALVRPRPHRPALPTEA
                     ALVVLREEVAAGRLAGKLVELLQELVTRDGPSAIGGDGNGAAGTGNGMAVRRGAAVTR
                     RAARGGLTLSV"
     gene            complement(4294..4941)
                     /locus_tag="VF995_04545"
     CDS             complement(4294..4941)
                     /locus_tag="VF995_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9342867.1"
                     /translation="MLVVDDHSVTRHGVVLLCRNAEGVEVIGEASDGNEAIERVGELL
                     PDVVLMDVDMPRKDGITATRQIRQDHPSVGVVVLTVHEDQETIFEAIKAGASGYLPKS
                     ATLDDIRSAVKAVAAGGSFLDPVQARKLLHQFNRYADETKAAADIYYLLTGREREILA
                     LLAEGLTSRQIASQLVISERTVNTHIGNIYRKLHVNNRVDAVREAMRIRLVEPPR"
     gene            complement(4980..5705)
                     /locus_tag="VF995_04550"
     CDS             complement(4980..5705)
                     /locus_tag="VF995_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9342868.1"
                     /translation="MTQSPTAPSLLGLEEDGRDLFGLAAHKLNTPLACIRGFVSTLLQ
                     RGNQLDAETVQQFLQLVLTQVDRLQGLVRDFLLLARVSGGIEALARPFRPRDVLLTLI
                     DDLGTEGVRVQPAGGVDAELVGDAELVRMALRPLVENGLAYGPRLGMVTVTVTAVPGG
                     SRWEVHDTGTRLSNEALSDLFRPFIRSDEPIERRGSGAGLGLTLARAYAEVLGGSVGG
                     RGGEGGTVFWLEIPTPTSEGVQR"
     gene            complement(5896..6705)
                     /locus_tag="VF995_04555"
     CDS             complement(5896..6705)
                     /locus_tag="VF995_04555"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX9342869.1"
                     /translation="MDPTLSHSEFEQLAAGFVLGALEPDDVETFEEHLRTCETCPPNL
                     RELEDVLGKLAYAAPAVEPPPGLRQAIMREVGGGRRRLALPRRRGRAGLAAAGAGAGA
                     GVRAAPRLAPRLAMAACVLALVAVSLWNVSLRHQNQQINGRLQGLALAAQLLTDSRTA
                     KVQLSGPAATTGTQATVVASSSRDQGVLLVQGLPPAAQRQVYELWSIPGGNLANATKA
                     GVFGAGSGVQAVAFALPIEPTTVFAVTEEPGPNGSPKPTTKPILAGQAGQA"
     gene            complement(6690..7319)
                     /locus_tag="VF995_04560"
     CDS             complement(6690..7319)
                     /locus_tag="VF995_04560"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9342870.1"
                     /translation="MPSWKDPERVRGARPYRGLDDGQLVALVAQRDAGALEALYERFG
                     KVAYSLARRILADETMAQDVVQEVFLSLWRDASRFDPGKGSVSTYLLSMTHHRAVDVV
                     RREENLRRHRTTDEVLEFEASRQDDVEAQVEAAERGAQVRGALGQLPAPQREALLLAY
                     FGGYTQREVAGLVGVPLGTVKTRMAAGMRKLRDALRDTGQQEQRPWTRH"
     gene            7493..7568
                     /locus_tag="VF995_04565"
     tRNA            7493..7568
                     /locus_tag="VF995_04565"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7526..7528,aa:Gly,seq:gcc)
     gene            7626..7700
                     /locus_tag="VF995_04570"
     tRNA            7626..7700
                     /locus_tag="VF995_04570"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7659..7661,aa:Cys,seq:gca)
     gene            7747..7821
                     /locus_tag="VF995_04575"
     tRNA            7747..7821
                     /locus_tag="VF995_04575"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7779..7781,aa:Val,seq:gac)
BASE COUNT         1134 a         2766 c         2948 g         1105 t
ORIGIN      
        1 ggggcggggg taggggccgc ggggggcggc acgaccggct gggatctgga ggggcgtgtg
       61 gcgccacggc gtggggtcgc gaccggcggt gcggcgggag gctgcgccga cgctggtgaa
      121 gccgccgggg cgcgcgcggg cgcggcctcc tccgggtgct ccgcgcgctc tccggatccc
      181 gaggcggcgc cggttccagg ggcgggtgct ggctggtgat cgggcagcgg cgcggtggcg
      241 atcgggccgc tgccggcacc gggcctgact cccgcgctcg gagtgttggg caggtcgcgg
      301 aggcggcgga accgttcctt cactggcagg cccggcaggg gcgaggcgtc ctcgggagcg
      361 actgcgacca ggtcgagtgg ctgctcagcg ccggccgccg ctacttgccg ctgggacggt
      421 ggcggcgcgg cgggagcggc cggagccgga gccgggggcg cgggtgcttg cctgcggccg
      481 cctccagcgg cgggttcgtg ccgcggtggc ggtggcggtg gctggtgcgg tggcgctggg
      541 gagcctggcg acggcgcggc cggtacgtcg tgaaggcggc gcaggcgccc gctccaggct
      601 ggctccgggt ctccgcccgg cttgcccaca gccgccgctg gcgcttcggg cgcaggcggt
      661 ggcggtggcg ggacggcggg aacctgggag ggcggcccct ccggctctcg ctcggtcttc
      721 cgctcggcct tccgctcggc cttccgctcg gtcttccgct cggtcttccg cgccggcggc
      781 gggaccggcg gcgcgggtgc cggcgccgga tacggggacg ccggcgcgcc gggggccacc
      841 ggcgggacca ccggcgcgga cggagacgcg gacggaggcg gcgcactcgc ccgggtgccg
      901 ggcggcgcga gcggcggctt ggcagccttg ggggcaggcc ccaccggggc ctcgcgccat
      961 ggcaccgcgg tgcccgtgcc cggggcggcg ctggcactgg cgctggcact ggcgccggtg
     1021 accagcgacc acagggtcat gatctcgaag gggcggcgca tgacggtgca ggcatgctcg
     1081 gcggggatca cgcccgcgtc ctcctcctgg ccgagcagca cgacgagcgg gccgcggaac
     1141 cgcgcccgga cctgctcgac gcccggcagg cgccagcccg ctgggaggtc gaccacggcg
     1201 gtgctcaccg gcagctcagc ggcgctcggc cactgggcgg ggcgatcgat ctcggtgacc
     1261 acgtcggctt cgccgagcag catgcgaagc gccaccgcca gcgagctgtc gcggctcaac
     1321 aggacgacgt gacgcggcac ccggccctac cttgccctcg tggaacgcat acgcataacc
     1381 ccgacccccc agtgcggctc agaccaccca gaatcaggat acgtgtgagc gtaggcgacg
     1441 gggcccgctt tgggaacggg gacgcccaag cggcgttcag gaacgctgaa acagcgaccg
     1501 gcgcgaacga ccgcgcccgg cgcgggcggg gcgccccggc gcggcggtgg cgagctggtc
     1561 ggcgagtctg cggatcgcct cggcgacgcc gctcttcggc tcctcgacca ggatcgggct
     1621 gcccttgttg atcgacagcg gcaccgcccg cgaggacggc agggtgacgt cgatctgggt
     1681 gtcgaggatc cgctcgacat cgggcaggcg cagcccgacc ttcgagtcgg cacggttgat
     1741 caccaggcag gtcttgtcgc gcgggtagcg cagcgactcc atagtccgca gggcgagctt
     1801 caggttcttg atgctcggca catcaagggt ggcgagcatg gcgatcgcgt cggattcatc
     1861 gaaggcggcc agcacctggt cggagaacgc agccggggtg tcgaccacca cgtagccgaa
     1921 gcgctgccgc agcgcccgca ggatggtcac caccgcggtc gggctgatgg tgtcggcgac
     1981 ggtcggctcg ctcggcgcca ccagcgccca caggccggac tggtggcggg tgaggaacga
     2041 cttcatggtg accgggtcaa ggccgccgcc gctggccctg gcggcgtcct ggatggtgcg
     2101 ggcgggaaac agctgcagca tgatcgcgac gtcgccgaaa tgcaggtcga ggtcgaccag
     2161 cgcgacctcc gcgtgggcgg cgagggcgag cgccaggttg gtggccacga aggtcttgcc
     2221 gcagccgccc ttggagctga agaccgtgat gagcttgcca gccttggccc gggcggggtc
     2281 ggcagcggcg gcggggcgac ggtcgggagc gccgccgagg ccgcgcagcg cccgggtcag
     2341 ctcgagcgcg cgggcgcagg cgccccgcac cgcggctggg tcgctcaggg cggtcaccac
     2401 gtcgcgcacg cccgcccgca gggcggcgcg gagcaactcg ccggagtcct gcccctcctg
     2461 ccgcagcagc accacggcca cgtccggtgc ggcctcctgg aggcgtccgg ccagggccac
     2521 cgcgtcggcg tcgctggaac cgggcccgac caccacgccg agcacgtccg ggccttcggt
     2581 ggcgaacgcc gccatggcct cgtcggggct ggtggtgcgg atgagctgtt cggccagctc
     2641 agccaggatg cccgcgagcc aggtcgcgga ctggtcgtcg gggtcgacga tgagtagggc
     2701 cacgggcctc cccatttcag gaattcctcc gccagacgag cgtggctggc gatgggcgga
     2761 cggtactggt ctgcctagcc cggagggaat ggggacagat acagaggcga gctgcgcata
     2821 actacggaaa ggtgtggatc agtccagcgt cgccagccgc actataccga cagcgtcagc
     2881 ccgccgcgag cggcccggcg cgtgactgcc gcaccgcgcc ggacggccat cccattgccg
     2941 gttccggcag cgccgttgcc gtccccgccg atggcggacg ggccgtcccg ggtcaccagc
     3001 tcctgcagca gctcgaccag cttcccggcg agccggccgg cggcgacctc ctcccgcagc
     3061 acgaccaggg ccgcctcggt gggcagcgcc gggcggtgcg gacgggggcg gaccagggcc
     3121 gcgtaggtgt cgaccaccgc gagcagctga gcgccgaacg ggatctgccg gcctcgcaac
     3181 ccccgcgggt agccggagcc gtcgagccgt tcgtgatgtg cccggacagc cgcgaccggc
     3241 acgccgacgc ggctgagcgg ctccaggatg tcggcggcga cgtcgggatg gcgccgcagc
     3301 agctcgacgg cggcgggatc cagcgcgcca ggcaccgcca gcgtgttccc tggcacgtcc
     3361 aacagtccga tgtcgtagaa catggcggcc agctccatgc cgcggacgcg catcggcgac
     3421 cagccgagct cgacggtcat ctcgcgggtg aggatggccg ccagacgggc atggccttcg
     3481 cgcccgtggc cgctcgcctc ggcggtgccg atcatcgtgc cgaggatcac ctggagggag
     3541 tgcgccagcg actcgcgggc cgagcgcagg tgctcgcgct cctcgctgcg gttgacccgc
     3601 gccgcggcgg tctcggtgcg cagcagcgcc tcctcggccg cctcggtcac cgagcgcaac
     3661 gtcgccagcg gcgagaccgg ctcgacccgc tcggggaccc gatgcgggtc gtggcggcca
     3721 cgcatcgacg aggcgacgcg gatcaccagg tcctcggcgc tgcaggggta ggtgagcagg
     3781 tcggtcacgc cgagcgcgag ggcgggcgtg aaatcgattg gggcgtcgcg gccaaccacc
     3841 ccgaggaccg gcacgcgggg ggtcatctcg cccaggcgct ggagcaccgg cgtccacacc
     3901 gtgacctgct cgacctggac cgccaccagc gccggccgct gcgcgtcgag gctgcggcca
     3961 gcctcgtcat cctcgcgggc gagcgtgcag gcgagccctg cgtccagcag ggcgtcacgc
     4021 atccacaggc cgatcccgtc ggcggaatcc aggacgagca cggggacgga caaaggcgac
     4081 tcccaaggtc acattggcca aggtcacatt ggcgaggggt ggcgcaaacg accgctgacc
     4141 aacaccactg cccagatgct acgcacttgt ggtgcttcgc cccatggacc gaactcgtga
     4201 tcgccactac cgagttctgc gcagactctc gggcgttgag ccgcccagca gcaggttcgg
     4261 ggagcctcgg cagcttccgg ggcgctgcgc ggctcagcgg ggcggttcga ccagccggat
     4321 ccgcatcgcc tcgcggaccg cgtcgacgcg gttgttgacg tgcagcttgc ggtagatgtt
     4381 gccgatgtgg gtgttcaccg tacgctcgga gatgaccagc tggctggcga tctgccgcga
     4441 ggtgagccct tcggccagca acgcgaggat ctcgcgttcc cggccggtga gcaggtagta
     4501 gatgtcggcg gcggctttgg tctcgtcggc gtagcggttg aactggtgca acagcttgcg
     4561 tgcctggacc gggtcgagga acgagccgcc ggcggcgacc gccttgaccg ccgagcggat
     4621 gtcgtcgagc gtcgccgatt tcggcaggta cccggaggcg ccagccttga tcgcctcgaa
     4681 gatcgtctcc tggtcctcgt ggacggtcag gaccacgacg cccaccgagg ggtggtcctg
     4741 gcggatctgc cgggtggcgg tgatgccgtc cttgcggggc atgtcgacgt ccatcaggac
     4801 gacgtcgggc agcagctcgc cgacccgttc gatcgcctcg ttgccgtcgc tcgcctcgcc
     4861 gatgacctcg acgccctcgg cgttcctgca gagcaggacc accccgtgcc gggtgacgga
     4921 gtggtcgtca acgaccagca cacgggtcgc ggtactgcca ttacggtcgc cgccgtcgct
     4981 cacctctgca ctccctcgga tgttggcgtg gggatctcca accagaacac ggtgccgccc
     5041 tcgccgccac gtcccccgac ggagccgccg agcacctcgg cgtaggcgcg ggcgagggtg
     5101 agcccgaggc ccgccccgga gccgcgccgt tcgatcggct catccgagcg aatgaagggc
     5161 cggaacaggt cgctgagcgc ctcgttggac agacgcgttc cggtgtcgtg cacctcccag
     5221 cgggagccgc ccggcacggc ggtgacggtg acggtgacca taccgaggcg gggcccgtag
     5281 gccaggccgt tctcgaccag cgggcgcagc gccatgcgga ccagctcggc gtcgccgacc
     5341 agctcggcgt cgaccccgcc ggcgggctgc acccggaccc cctcggtccc gaggtcgtcg
     5401 atcagggtga ggagcacgtc acgggggcgg aacgggcgcg cgagcgcctc gatcccgccg
     5461 ctgaccctgg cgagcagcag gaagtcgcgc accagaccct gcagccggtc gacctgggtg
     5521 aggacgagct gcaggaactg ctgcacggtt tccgcgtcga gctggttgcc gcgctgcagc
     5581 agcgtggaga cgaagccgcg gatgcacgcc agcggcgtgt tgagcttgtg cgcggccaag
     5641 ccgaaaagat cgcgcccgtc ctcctcgagg cccagcagcg agggggccgt ggggctctgg
     5701 gtcataggcc gcgttccctc cacgggagaa acatcacgtc cgggcattcg cgaacacctt
     5761 acgtctgcaa ttgtcgtaca gatagaggct gcctaccaac atagctacca gaaccactcg
     5821 cgatgactat ttcagagata gtctcccgag gccagcatct ggatgggagc gacattccat
     5881 ccgcagcgtc cggcctcatg cctgacccgc ttgacctgcc aggataggct tcgtcgtcgg
     5941 tttggggctg ccattcgggc cgggttcctc ggtgaccgcg aacaccgtgg tcggctcgat
     6001 cggcagtgcg aaggcgaccg cttggacgcc gctaccggca ccgaataccc ctgccttggt
     6061 ggcattggcc aggttcccgc cagggatgct ccagagctcg tagacctgcc gctgggcggc
     6121 gggagggagc ccctggacca gaaggacgcc ctggtctctg gacgaggagg cgaccacggt
     6181 ggcctgggtc ccagttgtgg cggccggccc gctcagctgg accttggcgg tccgcgagtc
     6241 ggtcagcagc tgggcggcca gcgccaggcc ttgcaggcgg ccgttgatct gctggttctg
     6301 gtgccgcagc gacacgttcc acaaggagac ggccaccaac gccagcacgc acgcggccat
     6361 ggcgagccgg ggggcgagcc ggggggcggc acgcacaccc gcgcccgcgc ctgccccggc
     6421 ggcggccagc ccggcacggc cgcgacgccg cggcagcgcc agccggcgac gcccgccgcc
     6481 gacctcacgc atgatcgcct ggcgcaggcc cggcggtggc tcgaccgcgg gggccgcgta
     6541 ggcgagctta ccgagcacgt cctcaagctc gcgcaggttc ggcgggcagg tctcgcaggt
     6601 ccgcaggtgc tcctcgaacg tctccacgtc gtccggttcg agcgccccga gcacaaagcc
     6661 cgctgcgagc tgctcgaact cgctgtggct cagtgtcggg tccatggtcg ctgctcctgt
     6721 tggccggtgt cgcgcagcgc gtctctcagc ttgcgcatcc cggcggccat cctggtcttc
     6781 acggtgccga gggggacgcc caccaagccg gcgacctctc gttgtgtata acctccaaag
     6841 taggcgagca gcagcgcctc acgttgcggc gccggcagct gccccagcgc gccgcgcacc
     6901 tgggcgccgc gctcggccgc ctccacctgc gcttcgacgt cgtcctgccg gctcgcttcg
     6961 aattccagca cctcgtcggt ggtgcggtgg cggcgcaggt tctcctcgcg gcgcacgacg
     7021 tcgaccgccc ggtggtgggt catcgacagc aggtacgtcg agaccgaccc cttccccggg
     7081 tcgaaccggc tggcgtcacg ccaaagcgac aagaagacct cctggacgac gtcttgggcc
     7141 atcgtttcgt cggccaggat ccggcgcgcc agggagtagg caaccttgcc aaaacgctcg
     7201 tagagcgcct caagggcacc agcgtctcgt tgcgcgacga gcgcgacaag ctgcccgtcg
     7261 tcgagacccc gatatggcct ggctcctcgc acccgctcag gatccttcca gctaggcatt
     7321 cgatccggaa ccgctcttgg attgcgcccc cccagcgggc cagcgacggc acagccagag
     7381 tcggcacgga cgctaacatt cagacccggg ggcggcaacg aggcaggacg ccggaaccgg
     7441 tcggaatggt tgaggaccgg gcgctggcgt tgctatagtt cgctcctcgc atgcggatgt
     7501 ggctcagttg gtagagcatc accttgccaa ggtgagggtc gcgggttcga gtcccgtcat
     7561 ccgctccacc tccgagcagc cagcgccctc cggcaccccg gagggcgctc tgccgaaggc
     7621 gacacggcgg tgtggccgag tggttcaggc aagggtctgc aaaaccctgt accccggttc
     7681 aattccgggc accgcctcca tccgtatcgc aacggtgtcc cagaccccgt aagatcggac
     7741 ccacccgggc gcttagctca gcgggagagc gcttccctga cacggaagag gtcactggtt
     7801 caatcccagt agcgcccacc accaataacc gcaggtaggg ccgggtcttg gaacctggcg
     7861 tcacggccgg ggcgctcatg gacggggctt tgggcagcaa acgggcagca tctacgcagc
     7921 aaggtgtgct ggcatggagg tccgtgaccg att
//
LOCUS       DASWDC010000142         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_100932, whole genome shotgun sequence.
ACCESSION   DASWDC010000142 DASWDC010000000
VERSION     DASWDC010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100932"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VF995_04580"
     CDS             <1..266
                     /locus_tag="VF995_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342871.1"
                     /translation="YFAEPGARNWARYSLATGIFEVSFFIGSTIVGAMDENGVIHNAP
                     TGILQRISIVTGWIWLSLVAYRELRALPQTAEAAAEQPRVVTA"
     gene            complement(303..1922)
                     /locus_tag="VF995_04585"
     CDS             complement(303..1922)
                     /locus_tag="VF995_04585"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX9342872.1"
                     /translation="MPTLLAEIAATSDAVASASARLAKIDRLAGCLRRLEPHEVDIAV
                     AFLSGELRQRQIGVGYAALRDLQIDPSGSPGPGARGGDPGHAATLTLAAVDAAFEQIG
                     RQTGPGSQAARRQLLAALFGAATPPETRFLVGLLSGELRQGALEGVMVEATARAAAVP
                     TREVRRALMLRGALGPVAEAALTQGVPGLRAFHLRVGRPLQPMLAQTATSVDQALERI
                     SPAAVEWKLDGVRVQIHRDGNEVAVFTRTLDDITARVPEVVEVALALPVRAAVLDGEA
                     IALHLDGRPHPFQVTAGRIGSKLEVERVRASVPLATFLFDVLHLDGEDLIDRGGAARG
                     EALVAAAPEPLRIPRLVTGEAEAAAAFLDDTLARGHEGVVVKSLEAPYEAGRRGAGWL
                     KVKPRHTLDLVVLAAEWGHGRRRGWLSNLHLGARDPRSGEFVMLGKTFKGLTDELLTW
                     QTERLQQLAVDQEQDQGGWVVRVRPELVVEVAFDGVQSSPRYPGGLALRFARVLRYRP
                     DKAAAEADTIDLVRAIHEGTPPRAKGGDDAG"
BASE COUNT          280 a          713 c          866 g          286 t
ORIGIN      
        1 gctacttcgc cgagccgggc gcgaggaact gggcccgcta ctcgctcgcc accgggatct
       61 tcgaggtgag cttcttcatc ggctccacca tcgtcggcgc gatggacgag aacggcgtca
      121 tccacaatgc cccgaccggc atcctccagc gcatcagcat cgtcaccggc tggatctggc
      181 tctccctggt cgcctaccgc gagctgcgcg ccctcccaca gaccgccgag gcggccgccg
      241 agcagccccg cgtcgtcacc gcgtagccgc gtcgcggcgc gagccgccac gcccgaccgg
      301 ccctaccccg cgtcatctcc ccccttggcg cggggtgggg ttccttcgtg gatggcgcgg
      361 accaggtcga tggtgtcggc ctcggcggcg gccttgtcgg ggcggtagcg cagcacccgg
      421 gcgaagcgga gggccaggcc gccggggtag cgggggcttg actggacgcc gtcgaaggcg
      481 acctcgacga ccagttcggg gcggacgcgg acgacccagc cgccctggtc ctgctcttgg
      541 tcgacggcga gctgctggag gcgttcggtc tgccaggtga gcagctcgtc ggtgaggccc
      601 ttgaaggtct tgccgagcat cacgaactcg ccgcttcggg ggtcgcgggc gccgaggtga
      661 aggttgctga gccagccgcg gcggcggccg tggccccatt cggcggccag gacgaccagg
      721 tcgagggtgt ggcgaggttt caccttgagc cagccggcac cccggcggcc cgcctcgtag
      781 ggggcctcca gcgacttcac caccacgccc tcatggccgc gggcgagggt gtcgtcgagg
      841 aacgcggcgg cggcctccgc ctcgccggtc accaggcggg ggatgcgcag cggctccggc
      901 gcggccgcca cgagcgcctc gccgcgcgcg gcgccgccgc ggtcgatcag gtcctcgccg
      961 tcgaggtgca ggacgtcgaa caggaaggtc gccagcggca ccgaggcccg gacccgctcg
     1021 acctccagct tggagccgat ccggccggcg gtcacctgga atgggtgggg gcggccgtcg
     1081 aggtgcaggg cgatcgcctc gccgtcgagc acggcggccc gcaccggcag ggccagggcg
     1141 acctcgacca cctcgggcac ccgggcggtg atgtcgtcca gggtgcgggt gaacaccgcg
     1201 acctcgttgc cgtcgcggtg aatttgcacg cggacgccgt cgagcttcca ttcgacggcg
     1261 gcggggctga tgcgttccag cgcctggtcg acgctggtgg cggtttgggc cagcattggc
     1321 tggagggggc ggccgacccg caggtggaag gcgcggaggc cggggacgcc ctgggtgagg
     1381 gcggcctcgg cgaccgggcc gagggcgccg cgcagcatca gggcgcggcg gacctcgcgg
     1441 gtggggacgg cggcggcgcg ggcggtcgcc tcgaccatga cgccttcgag ggcgccctgg
     1501 cggagctcgc cgctgagcag gccgaccagg aagcgggtct cgggaggggt cgcggcgccg
     1561 aacaacgcgg cgagcagctg gcgccgtgcg gcctgggagc cggggccggt ctgccggccg
     1621 atctgctcga aggcggcgtc gacggcggcg agggtgagcg tggcggcgtg gccggggtca
     1681 ccgcctcggg cgccggggcc ggggctgcct gatgggtcga tctggaggtc gcggagggcg
     1741 gcgtagccga cgccgatctg gcgttggcgc agttcgccgg acaggaaggc gacggcgatg
     1801 tcgacctcgt ggggttcaag gcggcggagg cagccggcga ggcggtcgat cttggccagg
     1861 cgcgcggagg cggacgcgac cgcgtcggag gtggcggcga tctcggcgag cagcgtgggc
     1921 atggcgccat tgtgcccggg taccaggaca agcgggttca gggactcctt caggattccc
     1981 gggatacctg ccctggtcgg aacacttgcg cagaaccatg caatggaggc cgccgccgtt
     2041 ccgctcgcct tggtgagctg agagaatgcg ccggatttgg gaagaacttt cggggggaca
     2101 tggggggacg agacggtgac gatcggatgg ccgaccgggg agggc
//
LOCUS       DASWDC010000143         6084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_936504, whole genome shotgun sequence.
ACCESSION   DASWDC010000143 DASWDC010000000
VERSION     DASWDC010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936504"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VF995_04590"
     CDS             <1..279
                     /locus_tag="VF995_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342873.1"
                     /translation="SGPSAVITPDGVAHQRTGLYTETTIRAQVAPRSGLTLYARFGRL
                     FEAALYLLAALTVAAVGLLQFRELRSRERERATATPEVAAGTSPGSGQ"
     gene            276..1082
                     /locus_tag="VF995_04595"
     CDS             276..1082
                     /locus_tag="VF995_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX9342874.1"
                     /translation="MTAKAIARALVVIPTYNEAESIDEAISRVLAADPRADVLVVDDG
                     SPDGTAKLVADLINAGPEPARLLLLERDAKAGLGAAYRAGFAWGLDRGGYDAFVEMDA
                     DLSHPPERLPALLDGLADADLVIGSRYVPGGRTVNWSRLRAALSRGGNAYVRLALGVP
                     IHDCTAGYRAYRRQVLEALPVAEVRSNGYCFQVELAHRTWQEGFRVAEVPITFTERAS
                     GVSKMSRQIVAEALWRVTVWSITGGRRRTRGRHPDSVAATRPRPRGKAAQ"
     gene            1108..1869
                     /locus_tag="VF995_04600"
     CDS             1108..1869
                     /locus_tag="VF995_04600"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEX9342875.1"
                     /translation="MTVAGARGRRAPWLGLVVALAAAAGAVDAISYLMAQVFTANMTG
                     NVVLLGLAAARHDTAAALRSGIAFLGFAAGAAAGGWVASWSTRGHEGRDGGGAGWPWP
                     GAVVAALLLEVPLMVAAEALLLAAGGERLGTAPRDALVVLTGAAMGAQSAAMRRVALP
                     NVATTYVTGTLTALLATTRGGSRRPSHRPLQAAVIAVYSAAAFAAGALERGVGPAAGF
                     AVAVVAFLAVAATAVAGWWRAPHRVSEPPVPGRAR"
     gene            complement(1835..2335)
                     /locus_tag="VF995_04605"
     CDS             complement(1835..2335)
                     /locus_tag="VF995_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9342876.1"
                     /translation="MLSSSSGGTAVLDEIDRAILAALLVNGRVSFRELSERIHLSPNA
                     IAERIRRLQRVGVIAAVRAELDLTALGRPLLALLDIRLVPGTRPEHFERTVRHLLGVL
                     DAVHVTGDFDYQLRVACRDANDLDDLVRRLKEQAGVRDSAIRIVLRQVDVDATLTLLG
                     PEPAAR"
     gene            2409..2990
                     /locus_tag="VF995_04610"
     CDS             2409..2990
                     /locus_tag="VF995_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342877.1"
                     /translation="MNGPTRWKRPATRPLAPVARFRPGLAPYWHGIMPGGTWRSGGRR
                     RAGSSGRARVVTRWRRRPPDPAVEAAWASFLRFAQALDEASRALLATVPSARRPGVPL
                     DASVAGFLAGLDAARAELPNWQPPALRERLGERRAATIAALDAAERRARELPAATAGL
                     DYEHRNELITETLDELLPIQDAERALRALRRGR"
     gene            complement(3077..3397)
                     /locus_tag="VF995_04615"
     CDS             complement(3077..3397)
                     /locus_tag="VF995_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342878.1"
                     /translation="MRRPSLREEIPHLNPAQLCAIRNVARAGGRVSSRALPEEVVSSL
                     LAARALRVERAVRQHRPGSDLRLTHLGLLALVQAANQFSGPDAGFCAVVLDRGGMVSS
                     LEKA"
     gene            3600..4646
                     /locus_tag="VF995_04620"
     CDS             3600..4646
                     /locus_tag="VF995_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9342879.1"
                     /translation="MGRPSKPLDPSSSQEARLGAAIRHRREQRGITQEQLAEAIGFSR
                     PHIALVELGRAHASEELVRRCDEVLEADGSVLVIYPEVVAERERRQQEAARLRSRPAP
                     RPLPASGRERDRERDADEERAPGRRPDRAQLAAFFGSSRRVVITIPLRTLRTGRLVIA
                     YEDVEAAETLARFIRGAGIDTRLRYVDPSGELDFSPAGLVVICGTKSSPVIAGLVEKH
                     DPLYACRPDEHGRVWIIERDTENRLGSPMDLGYPANQDICYFARLPRPGTDRPFLSIG
                     GIHAAGSLGAVHYLTNPQRFHDLYQAVEANRFTMVISSAFTRAPLRILSSEALTAPRL
                     HPRDLGCHHDAGRA"
     gene            4643..5593
                     /locus_tag="VF995_04625"
     CDS             4643..5593
                     /locus_tag="VF995_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342880.1"
                     /translation="MNSTRTVRSQSNPDTASERMSAEITLGSAPATGKPASEDLVVAA
                     DGIADGVTMVLDGASIPLGMAACCQRDAGWYVRQLAARLLATLAADTLLDLRTALAAA
                     ILSVDQAHAATCPATCHDRDRLGPSAMVALTRRAGDRLDWLLLGDATLLIANDAVVAC
                     HSDRRLAAVGRELRARVRRQLRTGGGYASPAYHDALTTLVQTERTLRNTASGYWVAAA
                     DPTAAAHSRTGSCRIGRGPGEVRRFALLSDGAERAVGTFRLYGSWAALLDALAHDGPM
                     AIIAAVRAAEAGDPDGRRYPRTRSSDDASALVCDFGPPAS"
     gene            5678..>6084
                     /locus_tag="VF995_04630"
     CDS             5678..>6084
                     /locus_tag="VF995_04630"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9342881.1"
                     /translation="MEQRMEPAQPEAERPRTKEDVLPPGARHCFACGDDNPIGLHLTG
                     IRRVGDEVRATLHPRPEFQSYPGVLHGGLTATALDELMAYASIMLAGIWGATATMEVR
                     YRARVPYDASLELVAGVTGERARRVRAWAKLLLP"
BASE COUNT          817 a         2435 c         2077 g          755 t
ORIGIN      
        1 tccggcccga gcgccgtgat cacccccgac ggcgtcgccc accagcgtac cggcctctac
       61 accgagacca ccatccgcgc ccaggtcgcg ccccgaagcg gcctgaccct ctacgcccgc
      121 ttcggccgcc tgttcgaagc cgccctgtac ctgctggccg cgctgacggt cgccgccgtc
      181 ggcctgctcc agttccggga gctgcgcagc cgggagcggg agcgcgccac cgccaccccc
      241 gaggtggccg ccgggaccag cccggggagc ggccagtgac cgccaaggcg atcgccaggg
      301 cgctggtggt gatccccacc tacaacgagg ccgagtcgat cgacgaggcg atcagccggg
      361 tgctcgccgc cgacccgcgc gccgacgtgc tggtggtcga cgacggcagc cccgacggca
      421 ccgccaagct ggtcgccgac ctgatcaacg caggccccga accggcccgg ctgctcctgc
      481 tggagcgaga cgccaaggcc gggctcggcg ccgcctaccg cgccgggttc gcgtgggggc
      541 tggaccgcgg cggctacgat gccttcgtcg agatggacgc cgacctgtcc cacccgcccg
      601 agcgcctccc cgccctgctg gacgggctcg ccgacgccga cctggtgatc ggctcgcggt
      661 atgtccccgg cgggcgcacc gtcaactgga gccgcctgcg cgcggcgctg tcgcggggcg
      721 gcaacgccta cgtccggctc gcgctggggg taccgatcca cgactgcacc gccggctacc
      781 gcgcctaccg ccgccaggtc ctggaggcgc tcccggtcgc cgaggtccgc tccaacggct
      841 actgcttcca ggtcgagctg gcccaccgga cctggcagga aggcttccgc gtggcggagg
      901 tcccgatcac cttcaccgaa cgcgccagcg gcgtctccaa gatgagccgc cagatcgtcg
      961 ccgaagccct ctggcgcgtg accgtctggt ccatcaccgg tggccgccgc cgcacccgcg
     1021 gccggcaccc cgacagcgtc gccgcgaccc gcccacgccc gcggggcaag gccgcccagt
     1081 gaccgcatgg accgaacggg gagcgtcgtg acggtggcgg gtgcgcgggg gaggcgggcg
     1141 ccctggctgg gcctggtggt ggcgctggcc gcggccgccg gggcggtcga cgcgatcagc
     1201 tacctgatgg cgcaggtctt caccgccaac atgaccggca acgtggtcct gctgggactt
     1261 gccgccgccc gccacgacac cgccgccgcg ctgcgctccg ggatcgcctt tctcggcttc
     1321 gccgccggcg ccgccgccgg cggctgggtg gcctcctggt cgaccagagg acacgagggc
     1381 cgggacggcg ggggagcggg ctggccgtgg cccggggcgg tcgtcgcggc actgctgctg
     1441 gaggtaccgc tgatggtcgc ggccgaagcc ctgctgctgg ccgccggcgg cgagcggctc
     1501 ggcaccgcgc cacgcgatgc gctggtggtg ctgaccggcg ccgcgatggg ggcgcagagc
     1561 gccgcgatgc ggcgggtggc gctgcccaac gtcgccacca cctacgtgac cgggaccctg
     1621 accgcgctgc tggccacgac ccggggcggg tcgcgcaggc cgagccaccg tccgctgcag
     1681 gcggcggtga tcgcggtcta ctccgccgcc gcgttcgccg ccggagccct ggagcgcggc
     1741 gtcgggcccg ccgccgggtt cgccgtggcg gtggtggcat tcctcgccgt ggccgccacc
     1801 gcggtggcgg ggtggtggcg cgcgccgcac cgggtcagcg agccgccggt tccgggccga
     1861 gcaaggtgag ggtcgcgtcg acgtcgacct gccgcagcac gatccggatc gcgctgtcgc
     1921 gcacacccgc ctgctccttg aggcgtcgga ccaggtcgtc caggtcgttg gcgtcgcgac
     1981 aggcgacccg cagctggtag tcgaagtcgc cggtgacgtg caccgcgtcg agcacgccga
     2041 gcaggtggcg gacggtgcgc tcgaaatgct ccggccgggt ccccggaacc aggcggatgt
     2101 cgagcagcgc gagcagcggc cggccgagcg cggtcaggtc cagctcggcg cggaccgcgg
     2161 cgatcacgcc gacacgctgc agccggcgga tgcgctccgc gatggcgttc gggctcaggt
     2221 ggatccgctc ggacagctcg cggaaactga cgcggccgtt caccaggagt gctgcgagga
     2281 ttgcgcggtc gatctcgtcc agcactgccg ttccccccga cgaactggac agcacctgca
     2341 gaaaccttgc actaggaaga tcttgccatg aatatgcggt cgatgcgaga actatttcgc
     2401 atgtcgggat gaacggccca acccgctgga agcgcccagc gacgcggccg ctcgcgccgg
     2461 tcgctcgctt ccgccctggc ctcgcgccgt actggcacgg catcatgcct ggcggaacct
     2521 ggaggagcgg cgggcgccgc cgagcgggat cgagcgggag ggcgcgggtg gtgacgaggt
     2581 ggcggcggcg cccgccggac ccggcggtcg aggcggcctg ggcgagcttc ctgcggttcg
     2641 cccaggcgct cgacgaggcc agcagggcgc tgctcgccac ggtcccgtcc gcgcggcggc
     2701 ccggcgtgcc gctcgacgcc tccgtcgccg gcttcctcgc cggcctcgac gccgcccgcg
     2761 cggagctgcc caactggcag cccccggccc tccgcgagcg gctcggcgag cgtcgtgcgg
     2821 ccaccatcgc ggcgctcgac gcggccgagc ggcgcgcccg ggaactcccc gccgccaccg
     2881 ccgggctcga ctacgagcac cgcaacgagc tgatcaccga gaccctcgac gagctgctcc
     2941 cgatccagga cgccgaacgg gccctgcgcg ccctgcgccg cggccgctga cgccagccac
     3001 ccccgcgccc agcgccgcgg tgcctgtcgg cggtttgttg gttgttcccc aaccggtcac
     3061 accgtgccct cggggttcac gccttctcca gtgaagacac cattccgccg cgatccagga
     3121 ccaccgcgca gaaccccgcg tccggtccag agaactggtt cgcggcctgc acgagggcga
     3181 gcagaccaag atgggtgagc cgcagatcac tgccgggccg gtgctggcgg accgcgcgct
     3241 cgacacgcag cgcgcgggcc gccagcaggc tgctcacgac ctcctcgggc agcgcccggc
     3301 tggaaacccg cccgccggcc cgggcgacgt tccggatcgc gcagagctgc gccgggttca
     3361 gatgtgggat ctcctcccgc aggcttggcc gcctcaccgg ttccaccggc tccctcgcgt
     3421 cgccgtcgcc gtcgccgtcg cgttcgcccc gcgcatgtcg cgccctcctc ctgccgagcc
     3481 aaccacccac caccgcgttc gaaggctcta gcagaccgaa caacgtccca ggttgtcgac
     3541 ggaaacaaag tcgtgtcggg aacaacaaac aaaggcacac aaccggtccg ggagcgctca
     3601 tgggtcgccc atccaaaccg ctcgatccgt cctcctccca ggaggctcgc ctcggcgcgg
     3661 cgatccgcca ccgacgcgag caacgcggca tcacccagga gcagctcgcc gaggcgatcg
     3721 gcttctcccg cccccacatc gcgctggtcg agctcggcag agcccatgct tccgaagagc
     3781 tggtccgccg ctgcgacgag gtcctcgaag ccgatggcag cgtcctggtc atctacccgg
     3841 aggtcgtcgc cgaacgagag cggcgccagc aggaagcggc ccggctgcga tcacggccgg
     3901 caccgcggcc cctgccagcc tctggccggg aacgcgaccg ggaacgcgac gccgacgagg
     3961 agcgcgcgcc cggccgccgg cctgaccgcg cccagctcgc cgccttcttc gggtccagcc
     4021 ggcgagtggt gatcaccatc cccctgcgga ccctgcggac cggtcgcctg gtgatcgcct
     4081 acgaggacgt ggaggccgcc gagaccctgg cgcggttcat ccgcggcgcc gggatcgaca
     4141 cccgcctgcg ctatgtggac ccctccggcg aactcgactt cagcccggct gggctggtgg
     4201 tcatctgcgg cacgaaatcc tcgccggtga tcgccggcct cgtcgagaag cacgacccgc
     4261 tgtatgcctg ccgtcccgac gagcacggcc gggtgtggat catcgagcgc gacaccgaga
     4321 accggctcgg ctccccgatg gacctcggct accccgccaa ccaggacatc tgctacttcg
     4381 cgcggcttcc ccggccaggc accgaccggc ccttcctgtc gatcggcggc atccacgccg
     4441 ccggctcgct cggcgccgtc cactacctca ccaaccccca gcgcttccac gacctctacc
     4501 aggccgtgga agccaaccgc ttcaccatgg tgatctccag cgccttcacc cgcgcgcccc
     4561 tgcggatcct ctccagcgag gcgctcaccg ccccccgcct gcacccccgt gacctcgggt
     4621 gccaccacga cgcggggcgt gcatgaacag cacccgcacc gtccgctccc agagcaaccc
     4681 cgacaccgcc agcgaacgca tgagcgccga gatcacgctc ggatccgcgc ccgcgaccgg
     4741 caagccggcc agcgaggatc tcgtcgtcgc ggccgatggc atcgccgacg gggtcaccat
     4801 ggtcctggac ggcgccagca tccctctcgg gatggccgcc tgctgccagc gcgacgccgg
     4861 ctggtacgtc cgccagctcg ccgcccggct cctcgccacc ctcgccgccg acaccctgct
     4921 cgacctgcgc accgcgctgg ccgccgcgat cctctcggtc gaccaggcgc acgcggccac
     4981 ctgcccagcg acctgccacg accgcgaccg gctcggcccc tccgcgatgg tggcgctcac
     5041 ccgccgcgcc ggcgaccggc tcgactggct gctgctcggc gacgcgaccc tgctgatcgc
     5101 caacgacgcc gtcgtggcct gccacagcga ccggcgcctc gccgcggtcg ggcgggagct
     5161 gcgcgcccga gtccgccggc agctgcgcac cggcggcggc tacgccagcc ccgcctacca
     5221 cgacgcgctc accaccctcg tccagaccga acgcaccctc cgcaacaccg ccagcggcta
     5281 ctgggtcgct gccgccgacc ccaccgccgc cgcccacagc cgcaccggca gctgccgcat
     5341 cgggcgcggc ccgggcgagg tccgccgctt cgccctgctc agcgacggtg ccgagcgggc
     5401 cgtgggtacc ttccggctct acggatcgtg ggcggccctg ctggacgccc tcgcccacga
     5461 cgggccgatg gccatcatcg ccgccgtccg cgccgccgag gccggcgacc ccgacgggcg
     5521 ccgctacccc cgcacccgct ccagcgacga cgcctccgcc ctggtctgcg acttcggccc
     5581 gccagccagc tgacgccccg cccggcagct tcctccggtc gtccgcgctc ggcccgtacc
     5641 ctggtcaggc aacggacggc aacggatgga gcaacggatg gagcaacgga tggagccagc
     5701 ccagccggaa gcggaacggc cacgcaccaa ggaagacgtc ctgccgcccg gtgcccgaca
     5761 ctgcttcgcc tgcggcgacg acaacccgat cggcctgcac ctgaccggca tccgccgggt
     5821 cggcgacgag gtccgcgcca ccctccatcc ccgccccgag ttccagagtt atcccggcgt
     5881 cctgcacggc ggcctgaccg ccaccgccct ggacgagctg atggcctacg ccagcatcat
     5941 gctcgccggg atctggggcg cgaccgcgac catggaggtc cgctaccgcg cccgggtccc
     6001 ctacgacgcg tcgctggagc tagtcgccgg cgtgaccggc gagcgagccc ggcgggtgcg
     6061 cgcctgggcg aagctgcttc tgcc
//
LOCUS       DASWDC010000144         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1368043, whole genome shotgun sequence.
ACCESSION   DASWDC010000144 DASWDC010000000
VERSION     DASWDC010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368043"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /gene="pgi"
                     /locus_tag="VF995_04635"
     CDS             complement(<1..918)
                     /gene="pgi"
                     /locus_tag="VF995_04635"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492394.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX9342882.1"
                     /translation="MTNGTGTTQITETPEWKALAEHHASMRGVHLRELFAADPTRGET
                     MTCEAGDLYLDWSKNRLTAETIALLVALAERAGLRERIEAMFGGERINVTERRPVLHV
                     ALRAPEGASIMVDGQDVVPEVHAVLRGMAAFAERVRSGAWLGVTGRPIRNVVNIGIGG
                     SDLGPAMAYEALRDYSDRSRRFRFVSNVDGTDIWEATADLDPAETLFIVSSKTFTTLE
                     TLTNAHSARDWLLGKLGGGEEAVRHHFVAVSTNAGEVARFGIDPDNMFGFWDWVGGRY
                     SFDSAIGLSLMIAIGPDRFRELLDGFHTVD"
     gene            complement(932..1411)
                     /locus_tag="VF995_04640"
     CDS             complement(932..1411)
                     /locus_tag="VF995_04640"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9342883.1"
                     /translation="MTYFTTFLSPRTPQPELRTLRLIAGRPHGLERLLLASGRMAAGY
                     HGYVHLHAGDEVVRVLGGEVVIRIDDERQTCHEGDVAVIPPNTLHGLRAVDDALVEIF
                     AERDMGTFFPVRQPDGSRRLVEIYRPDSPWNAPPPRPGGYTTDQELAAILRAIDVEI"
     gene            complement(1490..>2049)
                     /locus_tag="VF995_04645"
     CDS             complement(1490..>2049)
                     /locus_tag="VF995_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX9342884.1"
                     /translation="GYVGDFTADLPKVPRQGRRLVVLLGSTIGNLAPGERAALLAGVA
                     AACRPGDRFLLGVDLVKDRATLEAAYDDAAGVTAAFNRNVLRVLARELDAPIPVDAFE
                     HVARYDERQTRIEMRLRASKDLVLEFPALDLTVPLAAGEEVRTEICCKFTRSVVEREL
                     SAAGLALEGWHTDRRGQFALALAKR"
BASE COUNT          284 a          698 c          760 g          307 t
ORIGIN      
        1 gtcgacggtg tggaagccgt cgagcagctc ccggaaccgg tccgggccga tcgcgatcat
       61 cagcgacagg ccgatcgccg agtcgaagga gtagcggccg ccgacccagt cccagaatcc
      121 gaacatgttg tccgggtcga tgccgaacct ggccacctcg ccggcgttgg tggagacggc
      181 gacgaagtgg tgcctgacgg cctcctcgcc gccacccagc ttcccgagca gccagtcgcg
      241 ggcgctgtgg gcgttggtga gcgtctccag ggtggtgaag gtcttggagg agacgatgaa
      301 cagcgtctcg gccgggtcca ggtcggcggt cgcctcccag atgtcggtcc cgtcgacatt
      361 ggagacgaac cggaacctcc gcgagcggtc gctgtagtcc ctgagcgcct cgtaggccat
      421 ggcgggcccg agatcggagc cgccgatgcc gatgttgacg acgttgcgga tcggccggcc
      481 ggtcacccct agccaggcgc cggagcggac ccgctcggcg aaggcggcca tcccgcgcag
      541 caccgcgtgg acctccggca cgacgtcctg cccgtcgacc atgatggagg ctccctcggg
      601 ggcgcgcagg gcgacgtgca ggaccgggcg tcgttcggtg acgttgatgc gctcgccgcc
      661 gaacatcgcc tcgatgcgct cccgcagccc ggcgcgttcg gccagcgcca ccagcagcgc
      721 gatcgtctcg gcggtgagcc ggttcttgga ccagtcgagg tagaggtccc cggcctcgca
      781 ggtcatcgtc tcgccgcggg tggggtcggc ggcgaacagt tcccgcaggt ggacgccgcg
      841 catgctggcg tggtgctcgg cgagcgcctt ccactcgggc gtctcggtga tctgggtggt
      901 tccggttccg ttggtcatcg ctggagtcta ttcagatttc gacgtcgatc gcccgcagga
      961 tggcggccag ctcctggtcg gtggtgtacc cgccggggcg gggcggcggc gcgttccagg
     1021 gtgagtcggg gcggtagatc tcgaccagcc ggcggctgcc gtccggctgg cgcaccggga
     1081 agaaggtgcc catgtcccgc tcggcgaaga tctccaccag ggcgtcgtcg acggcccgca
     1141 ggccgtggag ggtgttgggc gggatcacgg cgacgtcgcc ttcgtggcag gtctggcgct
     1201 cgtcgtcgat ccggatgacg acctcgccgc cgaggacgcg gaccacctcg tccccggcgt
     1261 gcaggtgcac gtagccgtgg taaccggcgg ccatgcgccc gctcgccagc agcagccgct
     1321 ccaggccgtg cggccggccc gcgatcaggc ggagcgtgcg gagttccggt tggggcgttc
     1381 gcggtgacag gaatgtggtg aaatatgtca tgatactcac ggtcgcacca aagcgaccag
     1441 tggcgcaaga ctgtaatctg caccctactc ctccccccgc accggcctcc taccgtttgg
     1501 cgagggcaag ggcgaactgg ccccgccggt cggtgtgcca gccctccagc gccagccccg
     1561 cggcgctcag ctcccgctcc accaccgacc gggtgaactt gcagcagatc tcggtgcgga
     1621 cctcctcacc ggcggcgagc gggacggtca ggtcgagggc cgggaactcc agcaccaggt
     1681 ccttgctcgc ccgcaggcgc atctcgatgc gggtctggcg ctcgtcgtag cgggcgacgt
     1741 gctcgaacgc gtccaccggg atgggggcgt ccagttcgcg ggcgagcacc cgcaggacgt
     1801 tgcggttgaa ggcggcggtc accccggccg cgtcgtcgta ggccgcctcc agggtggcgc
     1861 ggtccttgac caggtcgacg ccgagcagga agcggtcgcc gggccggcag gcggcggcga
     1921 cccccgccag cagcgcggca cgctcgcccg gcgcgaggtt gccgatggtg gagccgagca
     1981 gcaccaccag gcggcggccc tggcggggca ccttgggcag gtcggcggtg aagtcgccga
     2041 cgtagccgt
//
LOCUS       DASWDC010000145         7188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_602335, whole genome shotgun sequence.
ACCESSION   DASWDC010000145 DASWDC010000000
VERSION     DASWDC010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602335"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2192
                     /locus_tag="VF995_04650"
     CDS             <1..2192
                     /locus_tag="VF995_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342885.1"
                     /translation="EAIEQNRREKDQLAALQALVSRHLEWAGLRDRLQLLRELARPAA
                     EHRDLAAEAEKVRVTVQTKRQEITADQAILDDLAKQAPALEAKLHSLVDESEQLAERL
                     EGAAEERRDLDTRVAALQARVDEARASALRLASARGTGERDASQAEAEAKAAESMLQT
                     TLARRREIAAELADRQSEAATLESGGVIVPPEVEAFRALLAEQGVHSAVVAELLEVTD
                     EADVATRAEAALGEALWALVVPADTYRDVTAMAASRGYRYGIVRAAAGEPHGVLAQVM
                     APGEVGLLLERYDALAALEAGQALSQSARGHVAVTGNGMHYSEAMARLQAPARPVLGR
                     NARDRHLAALRGEINRLSEELAKVDLAIPELRASWLKAMRLLESARNVSDQHAGTAQN
                     ERALEQAKAARPGLVEREAVLRRELRRIDERRGAAGAELDMARNMQAEVEGRLAKDLP
                     RLTELETRLTGFQAELEERALDPAQQAVLDAGDLPGTESLLRDLEWLQGQVDDLKRFP
                     NEVRDDGTLAARDAQARVVAEADALTEQLHQDLEAQQTRADSSTHRFDQRVRRVMDRL
                     AIEFARICEIAGWRGELHLLPGERPDDYGLDVLVAQRPGDRLRSYRDAAHSGGQRATI
                     AIVLLLAAMGAADAADLLLMDEHIAHLDSTNIDHVAALMHALGDRVQFLLATPTNAES
                     LRLSWCDLQLAFLPRDPGRAYSPPIRLLSRLGVGNLESRLPEGELAS"
     gene            complement(2564..3289)
                     /locus_tag="VF995_04655"
     CDS             complement(2564..3289)
                     /locus_tag="VF995_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX9342886.1"
                     /translation="MSLVARLVPAGSILVVGIAVAMLTRILITRVLRRVLPAQEGGPL
                     QPQSAANGVFWFLVLVTVIISLSLLAPSLLSNVPAHVIGFLPKLGTALVMLWLAALAA
                     GLVRGLAEGMLRRMNVAAGPLVAKSAYWAILGLGILMAADEIGVQTAVLTTVLYILMF
                     SIGLGTALAIGLGAWRLASNVIAGRYVDDRFAVGEQIQVEDYAGTITDVGIASITVAD
                     RDGSLVEIPHRWLLDRPVRRSGL"
     gene            complement(3308..3643)
                     /locus_tag="VF995_04660"
     CDS             complement(3308..3643)
                     /locus_tag="VF995_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342887.1"
                     /translation="MSAMEAASRPLSCGVDLVGLMWQVADGEPGDLAHQAGCQVCQAA
                     LVELRRLWVLVGELAAERVAAPDKIDRVTMQRIRRALFAAKVAEFFSGLLPRLGWALL
                     TYSGFARGR"
     gene            complement(3640..4182)
                     /locus_tag="VF995_04665"
     CDS             complement(3640..4182)
                     /locus_tag="VF995_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9342888.1"
                     /translation="MELQLDDDHLVTRAKAGRLDAFEELVRRHRTTVYRVALRMLGDE
                     GEAEDATQEAFVHASRGLAGFRSDARFSTWIHRIVTNRCLNLLRARRPAVPLPEVEVA
                     APPARAPERVVEVNWQLDDLKQAIDQLTPEQRAPLVLHDLEGHHYDEIAAMLDISVAA
                     VKSRLHRARLGLLDAMRQWR"
     gene            complement(4300..5592)
                     /locus_tag="VF995_04670"
     CDS             complement(4300..5592)
                     /locus_tag="VF995_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9342889.1"
                     /translation="MTAGIAAIGGATRRTFRSLQVRNYRLFFFGQLVSVSGTWMQQIA
                     QDWLVLRLTNRALPVGITTALQFTPVLLLAIWGGLVADRVDKRKLLLATQSVMASLAL
                     ALGVLTATGAVRLWMVYLLAALLGCATAFDMPTRQSFVTEMVGRDHVANAVGLNSAVF
                     NSARVIGPAAAGVLIAVTGIAPAFLLNAASYLAVIGGLRLMDPGELHRQAPVERSRGQ
                     VRDGLRYVRATPLLSWTILLMLVVSTFGLNYRVALPLLARFTFHGSAGLYGMLASVQA
                     LGAVVGALLTASRARPTRKLLAGSVAAFGLLSLLAAVVPNVLLEALVLAPIGFATVTA
                     MATANSTLQLGSAPEMRGRVMSLYGLVFLGSTPFGSALVGWLAERYGPRSALVLAGVS
                     SLLAAAVAALAARRKRDPAADRAPATPTAAETSSDEAA"
     gene            complement(5589..6071)
                     /locus_tag="VF995_04675"
     CDS             complement(5589..6071)
                     /locus_tag="VF995_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9342890.1"
                     /translation="MAPTPLPVPAEAQAEAQAPEVDRDLVVRLRLAIARLGRRLRQQT
                     GEEITYSQLSALGSIEKRGPLTLGALAAIEQVRPPSITRIVANLEEAGLVARQADPGD
                     RRIVRVEATPAGRELLRRGRIRKDAYLAARLAALTPAETELLRQAASVLERLLEVDLG
                     "
     gene            complement(6206..>7188)
                     /locus_tag="VF995_04680"
     CDS             complement(6206..>7188)
                     /locus_tag="VF995_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX9342891.1"
                     /translation="WFRTPRPEVRMRLCLMIEGQENVTWEQWLALAGACERHGLEGLF
                     RSDHYLSVFGQLERGSLDAWATLAGLAAHTSRIRLGTLVSPATFRHPSVLAKSVATVD
                     HISGGRVELGLGAGWYEAEHRAFGFEMPSSGTRLDLLAEQLEIVTRSWEAGPFSFSGA
                     HYRLEDVSPLPKPLQRPRPTLLIGGAAGPRSAELAARFADEYNTTAATIDDCKQRHDK
                     LASAWQQAGRDPDTLRFSLMTTTVVGRDRGELRDRLARVLERTGSGERPEDLLANPPE
                     RWILGTVDEVVERLHALEAAGVNRVMLQHLVHDDLDMVALLGSAVVPAVA"
BASE COUNT         1095 a         2604 c         2605 g          884 t
ORIGIN      
        1 tggaggcgat cgagcagaac cgccgggaaa aggaccaact cgccgcgctg caggcgctgg
       61 tcagccgcca tctggagtgg gccggcctgc gcgaccggct gcagctgctg cgcgagctgg
      121 cgcggccggc cgccgagcac cgcgacctcg ccgccgaggc cgaaaaggtc cgcgtcaccg
      181 tccagaccaa gcgccaagag atcaccgccg accaggccat cctcgacgac ctcgccaagc
      241 aggccccggc gctggaggcc aagctccact cgctggtcga cgagagcgag cagctggccg
      301 agcggctgga gggcgccgcc gaggagcgtc gcgacctgga cacccgcgtc gccgccctgc
      361 aggcccgggt ggacgaggct cgcgcctcgg cgctgcgcct ggcgtctgcg aggggcaccg
      421 gtgagcgcga cgccagccag gcggaagccg aggccaaggc cgccgagtcg atgctgcaga
      481 ccaccctggc gcggcgccgc gagatcgccg ccgagctggc cgatcgccag tccgaggcgg
      541 ccacgctgga gtccggcggg gtgatcgtcc cgccggaggt ggaggcgttc cgcgccctgc
      601 tcgccgagca gggcgtccac agcgcggtcg tggccgagct gctggaggtg accgacgagg
      661 ccgatgtcgc cacccgtgcc gaggccgcgc tcggcgaggc gctgtgggcg ctggtggtcc
      721 cggccgacac ctaccgggac gtgaccgcca tggccgcctc gcgcggctac cgctacggca
      781 ttgtgcgcgc ggcggccggc gagccgcacg gcgtgctcgc ccaggtgatg gcaccggggg
      841 aggtcggcct gctgctggag cgctacgacg ccctggcggc gctcgaagcc ggccaggcgc
      901 tcagccagtc tgcccgcggc cacgtcgcgg tcaccggcaa cgggatgcac tactccgagg
      961 cgatggcgcg gctgcaggct cccgcccggc cggtgctcgg ccgcaacgca cgggaccggc
     1021 acctggccgc gctgcggggc gagatcaacc ggctcagcga ggagctggca aaggtcgacc
     1081 tcgccatccc cgagctgcgc gcctcctggc tgaaggcgat gcgcctgctg gagtcggccc
     1141 gcaacgtctc cgaccagcac gccggcaccg cccagaacga gcgtgccctg gagcaggcca
     1201 aagccgcccg gcccggcctg gtcgagcgcg aggcggtcct gcggcgcgag ctgcggcgga
     1261 tcgacgaacg gcgcggcgcc gccggcgccg agctggacat ggccaggaac atgcaggccg
     1321 aggtggaggg ccgcctcgcc aaggacctgc cgcggctgac cgagctggag acgcgcctga
     1381 ccggtttcca ggcggagctg gaggagcgcg ccctcgaccc cgcccagcag gccgtcctcg
     1441 atgccgggga cctgcccggc accgagtcgc tgctgcgcga cctggagtgg ctccagggcc
     1501 aggtggacga cctgaagcgc ttccccaacg aggtgcgcga cgacgggacg ctggccgcgc
     1561 gcgacgcgca ggcgcgcgtg gtggcggagg ccgacgcgct caccgagcaa ctccaccagg
     1621 acctggaggc gcagcagacc cgcgccgaca gctcgaccca ccgcttcgac cagcgcgtcc
     1681 ggcgggtgat ggaccgcctc gccatcgagt tcgccaggat ctgcgagatc gccggctggc
     1741 gtggcgagct gcacctgctg cccggcgagc gtcccgacga ctacggcctc gacgtgctgg
     1801 tggcgcagcg gcccggcgac cggctgcgct cctaccgcga cgcggcccac tccggcgggc
     1861 agcgggccac catcgccatc gtgctgctgc tggccgccat gggcgccgcc gacgcggccg
     1921 acctgctgct gatggacgag cacatcgccc acctcgattc caccaacatc gaccacgtcg
     1981 ccgcgctgat gcatgcgctc ggcgaccggg tgcagttcct gctggccacc ccgaccaacg
     2041 ccgagtcgct gcggctctcc tggtgcgatc tgcagctcgc cttcctgccg cgcgacccgg
     2101 gccgcgccta ctcgcctccg atccggctgc tgtcgcgcct cggcgtcggc aacctggagt
     2161 cccgcctgcc cgagggagaa ctcgcctcct gagcactccc tcctgagcac tccgccgagc
     2221 aacaaacaaa ctggaaacaa agcggcagct aatacctcaa tcaaagttca acaaataaga
     2281 ggggacggag aggcgctgga ccgagcgccg tctccgtccc cctttgaggg cggcggggcc
     2341 gggctgccct acatccccgc aggtcagccg ctacccttga ggtgcagggg aggcccccct
     2401 cgcccgcccg cccacgctgg cagaggctcc gtccagcgga ttcccctacc aattcgcctg
     2461 actgattgct cggccagcct ggtcgatcga gctcagtcta ttcgacacgc gcctgcagga
     2521 aaagtttcgc ggaaatcttc cgccgatcgc ctgccgatcg cgtttacagc ccggagcgcc
     2581 gcaccgggcg gtcgagcagc cagcggtgcg ggatctcgac caggctgccg tcgcggtcgg
     2641 cgacggtgat gctggcgatg ccgacgtcgg tgatcgtgcc ggcgtagtcc tcgacctgga
     2701 tctgctcgcc gacggcgaag cggtcgtcga cgtagcggcc ggcgatcacg ttgctggcga
     2761 gccgccaggc accgagcccg atcgccagcg ccgtgccgag cccgatggag aacatcagga
     2821 tgtagagcac ggtcgtcagc acggcggtct gcacgccgat ctcgtcggcg gccatgagga
     2881 tgccgagccc gaggatcgcc cagtaggcgc tcttggccac cagcggcccg gcggcgacgt
     2941 tcatccgccg caacatcccc tcggcgaggc cgcgcaccag gccggcggcg agcgccgcga
     3001 gccacagcat caccagcgcg gtgccgagct tgggcaggaa gccgatcacg tgcgcgggca
     3061 cgttcgagag caggctcggt gccagcaggc tcagcgagat gatcacggtg accagcacca
     3121 ggaaccagaa caccccgttg gccgcgctct gcggctgcag cggcccgccc tcctgcgcgg
     3181 gcagcacccg ccgcagcacc cgcgtgatca ggatccgggt cagcatcgcg accgcaatgc
     3241 caaccaccag gatcgagccg gcgggcacca ggcgtgcaac caagctcacc gtgtcacctc
     3301 ttccccctca tcggcccctc gcgaacccgg agtaggtgag caaagcccat ccgagccgtg
     3361 ggagcaaacc actgaagaac tccgcgacct tcgcggcgaa cagcgcccgg cggatgcgct
     3421 gcatcgtcac ccggtcgatc ttgtccggcg ccgccacccg ctcagccgcc agctcaccga
     3481 ccagcaccca caggcgccgc agctccacca gggccgcctg gcagacctgg cagccggcct
     3541 ggtgcgccag gtcgccaggc tcgccgtcgg cgacctgcca catcaggccg accaggtcga
     3601 ccccacagct gagcggccgg ctggcggcct ccatggcgct catcgccact gcctcatcgc
     3661 gtccaggagc ccgaggcggg cccggtgcag gcggctcttg accgcggcga cgctgatgtc
     3721 gagcatcgcg gcgatctcgt cgtagtggtg gccctccaga tcatggagga cgagcggggc
     3781 gcgctgctca ggggtgagct ggtcgatcgc ctgcttcagg tcgtcgagct gccagttcac
     3841 ctcgaccacc cgctcgggcg cccgcgcggg cggcgccgcg acctcgacct cgggcagcgg
     3901 cacggcgggc ctacgggcgc gcagcaggtt caggcagcgg ttggtcacga tccggtggat
     3961 ccaggtcgag aacctcgcgt ccgagcggaa gccggccagc ccgcgcgacg cgtggacgaa
     4021 cgcttcctgg gtggcgtcct cggcctcccc ctcgtccccg agcatgcgca gcgcgacccg
     4081 gtacaccgtg gtgcggtggc gacggaccag ctcctcgaac gcgtcgaggc gtcccgcctt
     4141 cgccctggta accagatggt catcgtcgag ctgcagttcc atccaggccg gttcctgtcc
     4201 cctggttcga cagcatcacc atgggaaagt tacgcacacg agccgtgaaa ctctcaaacc
     4261 gcggacccga tccccacccg ccaaaggggc cggaggggtc taagcggcct catcagagct
     4321 ggtttcggcc gccgtggggg tcgccggggc ccggtcggcg gcggggtcac gcttccgccg
     4381 cgcggccagg gcggcgaccg cggcggcgag caggctggag acgccggcca ggaccagggc
     4441 cgagcggggg ccgtagcgct cggcgagcca gccgaccaga gcgctgccga agggggtgga
     4501 gccgaggaac accaggccgt agagggacat cacccggccg cgcatctctg gggcggagcc
     4561 gagctgcagg gtggagttgg cggtggccat cgcggtgacg gtggcgaacc cgatcggggc
     4621 gagcaccagc gcctccagca ggacgttggg gacgacggcg gccagcagcg acagcagccc
     4681 gaaggccgcc accgaaccgg ccaggagctt gcgggtcggg cgtgcccggc tggcggtgag
     4741 cagggcgccg acgaccgcac cgagcgcctg gaccgaggcg agcatgccgt agaggccggc
     4801 gctgccgtgg aaggtgaagc gggcgagcag cggcagcgcc acccggtagt tgagcccgaa
     4861 ggtgctcacc accagcatca gcaggatcgt ccacgacagc agcggggtcg cgcgcacgta
     4921 gcggagcccg tcgcgcacct ggccccggga gcgctccacc ggcgcctggc ggtggagctc
     4981 gccagggtcc atcagccgca gcccaccgat gaccgccagg taggacgccg cgttgagcag
     5041 gaaggcgggg gcgatcccgg tgaccgcgat cagcacgccg gcagcggccg ggccgatcac
     5101 ccgggcggag ttgaacacgg cgctgttgag gccgaccgcg ttggcgacgt ggtcgcggcc
     5161 gaccatctcg gtgacgaacg actggcgcgt cggcatgtcg aaggcggtgg cgcagccgag
     5221 cagcgcggcg agcaggtaga ccatccacag ccgcaccgcc ccggtggcgg tcagcacgcc
     5281 gagggcgagc gccaggctgg ccatcacgct ctgggtcgcc agcaggagct tgcgcttgtc
     5341 gacccggtcg gcgaccagcc cgccccagat ggcgagcagc agcaccgggg tgaactgcag
     5401 cgcggtggtg atgcccaccg gcagggcgcg gttggtgagc cgcagcacca gccagtcctg
     5461 ggcgatctgc tgcatccagg tgccgctgac cgacaccagc tggccgaaga agaacaggcg
     5521 gtagttgcgg acctggaggg agcggaacgt ccgccgcgtc gctcccccga tggccgcgat
     5581 cccggcggtc aaccgaggtc cacctccagc aggcgctcca gcacggaggc ggcctggcgg
     5641 aggagctcgg tctccgcggg ggtgagggcg gccaggcgtg cggccaggta ggcgtccttg
     5701 cggatgcggc cgcggcggag cagctcgcgg ccggcggggg tcgcctcgac ccggacgatg
     5761 cgccggtcgc cggggtcggc ctgtcgggcg accaggccgg cctcctccag gttggcgacg
     5821 atgcgggtga tgctgggcgg gcgcacctgc tcgatcgcgg cgagggcgcc aagggtgagg
     5881 ggcccgcgct tctcgatgct ccccagggcc gagagctgcg aataggtgat ctcctccccg
     5941 gtctgctggc gcaggcgccg gccgagccgc gcgatcgcca gccggagccg caccaccagg
     6001 tcacggtcga cctcaggcgc ctgggcctcc gcctgcgcct cggctgggac tgggagcgga
     6061 gtgggagcca tcaaacctcg ccatcaacga gccgggagta cttcgcaagg gtaattagca
     6121 ttgctaacta tcaacgccca aggtagcacg gtccgtccca tgtgccaagt ctgggttcca
     6181 ggtctgggtt ccgggtcagg ccacctcagg ccacggcggg gacgaccgcc gagccgagca
     6241 gggcgaccat gtcgaggtcg tcgtggacca ggtgctgcag catcacgcga ttcaccccgg
     6301 cggcctccag cgcgtggagc cgctcgacca cctcgtcgac ggtgccgagg atccagcgtt
     6361 ccggcgggtt ggcgagcagg tcctcgggcc gctcgccgct gccggtgcgt tccagcacgc
     6421 gggcgagccg gtcgcgcagc tcgccccggt cgcggccgac caccgtcgtg gtcatcagcg
     6481 agaaccgcag cgtgtccggg tcgcggccgg cctgctgcca ggcgctggcc agcttgtcgt
     6541 ggcgctgctt gcagtcgtcg atggtggcgg cggtggtgtt gtactcgtca gcgaagcggg
     6601 ccgccagctc ggcgctgcgc ggcccggcgg cgccgccgat gagcagcgtc gggcgcgggc
     6661 gctgcagcgg cttgggcaac gggctgacgt cctccagccg gtagtgggcg ccgctgaacg
     6721 agaacggccc cgcttcccac gaccgcgtga cgatctccag ctgctcggcc agcaggtcga
     6781 ggcgcgtccc gctggacggc atctcgaagc cgaaggcgcg gtgctccgcc tcgtaccagc
     6841 cggccccaag gccgagctcg acgcggccgc cggagatatg gtcgaccgtg gccacgctct
     6901 tggccagcac cgagggatgg cggaaggtcg ccggggagac cagcgtgccg agccggatgc
     6961 ggctggtgtg cgccgccagc ccggcgagcg tcgcccaggc gtcgagcgag ccgcgctcca
     7021 gctgcccgaa caccgacagg tagtggtcgg agcggaacag cccctccagg ccgtgccgct
     7081 cgcaggcgcc cgccagcgcg agccactgct cccaggtgac gttctcctgg ccctcgatca
     7141 tcaagcagag ccgcatgcgc acctccggac gcggggtacg aaaccaac
//
LOCUS       DASWDC010000146         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_435112, whole genome shotgun sequence.
ACCESSION   DASWDC010000146 DASWDC010000000
VERSION     DASWDC010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435112"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..420)
                     /locus_tag="VF995_04685"
     CDS             complement(154..420)
                     /locus_tag="VF995_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342892.1"
                     /translation="MAMLARAGTYSVIGYGGTLSLPSNPLVVNEHAALGNLVGSWPDL
                     WELLQLHATGRITLHSQTYPLDQVNDVLARLRVGDITGRAVLVP"
     gene            complement(591..1535)
                     /locus_tag="VF995_04690"
     CDS             complement(591..1535)
                     /locus_tag="VF995_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX9342893.1"
                     /translation="MVDPGAATVDDDSTTNVDLPRAQSGSQPQHLLITLLGDYWRDRA
                     EQLPSAALVRLVAEFGVTPVGARAALSRLTRRGLLASSKRGRRTYYGLTERAKRVLAE
                     GTQRIMAFGTSSTSWDGRWTVAAFSVPEDQRDLRHALRTRLRWLGFAPLYDGMWVSPG
                     DAVQAALEVLREVGVERATVMTATHQPGGPGGGDPIAAWNLDDLRKVYEEFIGGYEPL
                     LRRVRQGGVGAAEALIARTAVMDTWRNFPNLDPELPDELLPDGWPRRHARDIFAEVYN
                     ALGPLGEVRVRQVLAEFAPELTGLVHHHTTEITGHRNA"
     gene            1743..>2857
                     /locus_tag="VF995_04695"
     CDS             1743..>2857
                     /locus_tag="VF995_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342894.1"
                     /translation="MQALVRLALDVARRDRAAGLRTGVLVTGYPGSPISGLDLALQRE
                     AELAERHGVRLVPAAAEERAATALMGSQMLDGFPHSRYDGVVGFWYGKGPGIDRSGDA
                     LKHGNFAGTSEHGAVVVLSGEDHEAKSSTMPFQEDYAFVSAGIPVLYPSSVEEFRTLG
                     LHAVAMSRYSGCWVALKLVTALCDGGQSLQLVQDDPEIVLPELEIGGQPFRKRTDFTF
                     FPGRNIDHERHLYRERQVAALAYARANHLDRIVVRSEGRRRDQVGLVAAGKSWADLRQ
                     ALQDMGVDDDTLRAHGIRLLKLALTYPLDPEIVAEFAAGLDEIVVVEEKHGVVEWQVK
                     EALCGLPHPVRVLGKRDEHGAPLFPVEGGMDADRIAE"
BASE COUNT          404 a          959 c         1086 g          408 t
ORIGIN      
        1 cgagaaccgt gcctggcctg gcctgcggga gaagcctgcc ttcggcgatc cacgggtcga
       61 tcgcaggatt gccggtcagc ggtggcagct cgtcgggtgg acgcgtgttg gacgtagtca
      121 tgccacaact ctagtggcag cagaggcctg agcctacggg accaggaccg cgcggccggt
      181 gatgtcgccg acgcgaagcc gcgcgaggac gtcgttgacc tggtcgaggg ggtaggtctg
      241 ggagtggagg gtgatgcggc cggtggcgtg gagttggagg agttcccaga ggtccggcca
      301 ggagccgacg aggttgccga gggcggcgtg ttcgttgacg accagggggt tggaggggag
      361 ggagagggtg ccgccgtagc cgatgacgga gtaggtgccg gcgcgggcga gcatggccat
      421 gctgtcggcg tgggcgcgat ctcgtggggg gcgacgccct cggggagctt gatgagctcc
      481 cgctcggaga ccaggacgtg gtcggcgaag ccgccgtcgc gagacaggcc ggtgaactgg
      541 tggcggacgc aggatgttag ggagacgtca cccggtcgtc aaacgtcgtg tcatgcgttc
      601 cggtggccgg tgatctcggt ggtgtggtgg tggacgaggc cggtcagctc gggggcgaac
      661 tcggccagga cctggcggac gcggacctcg ccgagggggc cgagggcgtt gtagacctcg
      721 gcgaagatgt cgcgggcgtg gcggcggggc cagccgtcgg ggagcagttc gtcgggcagc
      781 tcggggtcga ggttggggaa gttgcgccag gtgtccatca cggcggtgcg ggcgatcagc
      841 gcctcggcgg caccgacccc gccctggcgg acgcggcgca gcagcggctc gtacccgccg
      901 atgaactcct cgtagacctt gcgcaggtcg tcgaggttcc aggcggcgat cgggtcgccg
      961 ccgccgggac cgcccggctg gtgggtggcg gtcatcacgg tggcgcgctc gacgccgact
     1021 tcgcgcagga cctccagggc ggcctggacg gcgtcgccgg gcgacaccca catgccgtcg
     1081 tacagcggcg cgaacccgag ccagcgcagg cgggtccgga gcgcgtggcg caggtcccgc
     1141 tggtcctccg gcaccgagaa cgccgccacg gtccagcgcc cgtcccagga cgtggagctg
     1201 gtcccgaagg ccatgatccg ctgggtgccc tcggcgagca cccgcttggc ccgctcggtg
     1261 aggccgtagt acgtccgccg gccgcgcttg gaggaggcga gcagcccgcg gcgggtgagc
     1321 cggctcaggg ccgccctcgc ccccaccggc gtcacgccga actcggccac caagcggacc
     1381 agcgccgccg aaggcagctg ctcggcgcgg tcccgccagt agtcaccgag cagggtgatc
     1441 agcaggtgct gcggctgcga cccgctctgc gcgcgcggca ggtcgacgtt cgtcgtggag
     1501 tcgtcgtcga ccgtcgccgc tcccgggtcc accatggcga tgagtgtacg cagtccgcac
     1561 gcccggaagc attcgcggcg ccgcctggcc gccgtttcaa cgaatggaac attcaataga
     1621 cggtcgttca ggccgatgca acacttcctg aaatggcagc tccagctcca gcgcccgcat
     1681 cgaatccgcc gagcttgcgg gatcggctga ccgagcccga gggcaggttc tacctcggag
     1741 gcatgcaggc cttggtgcgc ctggccctgg acgtcgcccg ccgcgaccgc gccgccggcc
     1801 tgcggacggg cgtgctggtc accggctacc ccggctcccc catctccggc ctggacctgg
     1861 ccctgcagcg cgaggccgaa ctggccgagc ggcacggcgt gcggctggtc ccggcggcgg
     1921 ccgaggagcg cgcggccacc gcgttgatgg gctcgcagat gctcgacggc ttcccccact
     1981 cccgctacga cggggtggtc ggcttctggt acggcaaggg tcccggcatc gaccgctccg
     2041 gcgacgccct caagcatggc aacttcgccg ggaccagcga acatggcgcg gtggtggtgc
     2101 tgtccggcga ggatcacgag gcgaagtcct cgaccatgcc cttccaggag gactacgcct
     2161 tcgtctcggc cgggatcccg gtgctgtacc cgtccagtgt cgaggagttc cgcaccctcg
     2221 gcctccacgc ggtggcgatg tcgcggtaca gcggctgctg ggtggcgctc aagctggtga
     2281 ccgccctgtg cgacggcggc cagagcctcc agctggtcca ggatgacccc gagatcgtgc
     2341 tgccggagct ggagatcggt gggcagccgt tcaggaagcg gaccgacttc accttcttcc
     2401 ccggccgcaa catcgaccac gagcggcacc tgtaccggga acgccaggtc gccgcgctcg
     2461 cctacgcccg cgccaaccac ctcgaccgga tcgtggtccg gagcgagggc cggcgccggg
     2521 accaggtcgg cctggtggcc gccgggaagt cctgggccga tctgcgccag gccctccagg
     2581 acatgggcgt cgacgacgac accctgcggg cgcacgggat ccggctgctc aagctggcgc
     2641 tcacctaccc tctcgacccg gagatcgtcg ccgagttcgc cgccggcctg gacgagatcg
     2701 tggtcgtcga ggagaagcac ggcgtggtcg agtggcaggt caaggaggcc ctctgcggcc
     2761 tcccccaccc ggtccgggtg ctcggcaagc gcgacgagca cggcgccccc ctgttcccgg
     2821 tcgagggcgg catggacgcc gaccggatcg ccgagat
//
LOCUS       DASWDC010000147         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1368942, whole genome shotgun sequence.
ACCESSION   DASWDC010000147 DASWDC010000000
VERSION     DASWDC010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368942"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /gene="tcuA"
                     /locus_tag="VF995_04700"
     CDS             complement(<1..1149)
                     /gene="tcuA"
                     /locus_tag="VF995_04700"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX9342895.1"
                     /translation="MVVVGGGNAAIVSALSARDQGARVLVLERAPLVFRGGNSRHTRN
                     IRCVHDHPDAYTPGVYGFGEMWADLCGIGGPGDERLAELTVRQSVSVPAWMSARGVRW
                     QPPLNGTLHLGRTNRFFLGGGKALLNAYYRTAGRAGVEVAYESGVEALRFDGDACVEV
                     IVNAPDGRRRVRAGAVVCASGGFEANIDWLRRYWGDAALNYHVRGPRYNDGTVLAALL
                     EHGAERAGEERGFHAVAVDARSPRFDGGIATRVDAIPFGIVVNRAGQRFCDEGEELWP
                     KRYATWGGHIALQPGQIAYALWDRKVLGRFIPPMYGAYQADSVAALAGQLGMAPAAVR
                     AAEDTVGVYNQAVDHGPGSRFDPAVLDGLGTAGLDPPKSNWAQRIDTPP"
     gene            complement(1271..2206)
                     /locus_tag="VF995_04705"
     CDS             complement(1271..2206)
                     /locus_tag="VF995_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9342896.1"
                     /translation="MSESQPWVSEVGDGIYAYIQPDGSWWINNTGFLLLPDGEGVVSV
                     DACSTERRTLAYRQAIARVTPAPVRTLINTHFHGDHTHGNVFFPTATIVAHERTRREL
                     LAAPPLEAYARLFEAPEWGAVRRVAPFLTYTDQVMVYAGELRCEVRYVGQPAHTGADS
                     IVWIPERRVLFAGDLVFHGGTPFVLMGSVAGTIAVSEDLKRLGAETVVPGHGDLCGPE
                     WFEVTIGYLRFVQDVARRGIEAGLTPLEAAREADLGEYKAWTDAERIVGNLHRAYAEL
                     GGLDGSGAGAYAEVDVLGALQQMVVFNGGPLHCHA"
     gene            2468..>3085
                     /locus_tag="VF995_04710"
     CDS             2468..>3085
                     /locus_tag="VF995_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342897.1"
                     /translation="MWTTHLRSSFPRRGIRTRLAVAIVGAVVVLLAVVAGPAAAAGSS
                     LIHLSTDPYTNSFSAHQTEVEPDSLAAGSTIVSAFQVGRVFIGASANIGWATSRDGGR
                     TWQHGFLPKTTTQASPPGSYARASDASVAWSARDHTWLISYLVAPSVTSNAIVDLLVS
                     RSRDGIHWGPPITVAARNEFLDKNWTTCDNSPASPFYGNCYTELEV"
BASE COUNT          446 a         1142 c         1040 g          457 t
ORIGIN      
        1 cggcggcgtg tcgatgcgct gcgcccagtt gctcttgggc gggtcgagcc cggcggtgcc
       61 gaggccgtcc aggacggcgg ggtcgaagcg gctccccggg ccatggtcaa ccgcctggtt
      121 gtagacgccg accgtgtcct cggccgcccg caccgccgcg ggcgccatgc ccagctgccc
      181 ggccagtgcc gcgaccgagt cggcctggta ggcgccgtac atcggcggga tgaagcggcc
      241 gaggaccttg cggtcccaca gggcataggc gatctggccg ggttgcaggg cgatgtggcc
      301 gccccaggtg gcgtagcgct tgggccagag ctcttcgccc tcgtcgcaga agcgctgccc
      361 tgcccggttg accacgatgc cgaaggggat cgcgtccacc cgggtcgcga tgccgccgtc
      421 gaacctgggg ctgcgggcgt cgaccgcgac ggcgtggaag ccacgctcct ccccggcgcg
      481 ctcggcgccg tgctccagca gcgcggcgag caccgtgccg tcgttgtagc gcgggccgcg
      541 gacgtggtag ttcagcgccg cgtcgcccca gtagcgccgc agccagtcga tgttggcctc
      601 gaagccgccg ctggcgcaga cgaccgcgcc cgcccgcacc cgccgccggc cgtcgggggc
      661 gttcacgatc acctcgacgc aggcgtcgcc gtcgaaccgc agggcctcga cgccggattc
      721 gtaggcgact tcgacgccgg cccgcccggc ggtgcggtag taggcgttca gcagcgcctt
      781 gccgccaccc aggaagaacc ggttggtgcg ccccaggtgc agggtcccgt tcagcggcgg
      841 ctgccagcgc acgccgcgcg cggacatcca ggccggcacc gagacggact gccgcaccgt
      901 cagctcggcg aggcgctcgt cgccagggcc gccgattccg cacaggtccg cccacatctc
      961 gccgaagccg tagacgccgg gggtgtaggc gtcggggtgg tcgtggacgc agcggatgtt
     1021 gcgggtgtgg cggctgttcc cgccacggaa caccagcggc gcccgctcca gcacgagcac
     1081 gcgcgcgccc tggtccctgg cgctgagcgc gctgacgatc gccgcgttgc cgccgccgac
     1141 caccaccacg tcccagcgcc catcgagcct cgccagcgga acccacctcc tccggtgttc
     1201 atgagaatcc tagtgcatac aacggtacac aacaagggcg acatgaacgt cggtgaggcg
     1261 gcgagcgacc ctaggcgtgg cagtgcagcg ggccgccgtt gaagaccacc atttgctgaa
     1321 gggcgcccag cacgtcaacc tccgcgtagg cgcccgcgcc ggaaccgtcc agcccgccca
     1381 gctcggcgta ggcgcggtgc aggttgccga cgatccgctc ggcgtccgtc cacgccttgt
     1441 actcgcccag gtcggcctcc cgggcggcct ccagcggggt cagcccggcc tcgatccccc
     1501 gccgggcgac gtcctggacg aagcgcaggt agccgatggt cacctcgaac cactccggcc
     1561 cgcagaggtc gccatgcccg ggaacgacgg tctccgcgcc gagccgcttg aggtcctcgc
     1621 tcaccgcgat cgtgccggcg accgaaccca tcagcacgaa cggcgtgccg ccgtggaaga
     1681 ccagatcccc ggcgaacagc acccgccgct cggggatcca cacgatcgag tcggccccgg
     1741 tatgggccgg ctggcccaca taccgcacct cgcagcgcag ctcgcccgcg tagaccatga
     1801 cctggtcggt gtaggtgagg aacggcgcca cgcggcgcac ggccccccac tccggcgcct
     1861 cgaagagccg cgcgtaggcc tccagcggcg gagccgccag cagctcccgg cgggtccgct
     1921 cgtgcgccac gatcgtggcg gtcgggaaga acacgttgcc gtgggtgtgg tcgccgtgga
     1981 aatgggtgtt gatcagcgtc cgcaccggcg cgggggtgac ccgcgcgatc gcctgccggt
     2041 aggcgagcgt gcgccgctcg gtggagcagg cgtcgacgct caccaccccc tcgccgtccg
     2101 gcaggagcag gaaccccgtg ttgttgatcc accagctccc gtcaggctgg atataggcat
     2161 agatcccgtc gccgacctca ctgacccagg gctggctctc gctcatgcat gacatcccgt
     2221 ctctgcgcgt tctcccgacc tgtcccctcg attatccggt ggtggtgaac agcgggtcga
     2281 ggtagccgtc cggcgcgagc gcgctgattc cttcgttgat tccttcgttg attccttctt
     2341 gacaagtagg gttcagagtg ctcgtctgcg tagatcgtga gattcatccc gatcgctcgc
     2401 gctccgccca ggttgcccgc aaatcacgtg gcatccatgc agagctgcag gcgcaaagga
     2461 gcgcggcatg tggacgacgc atctgcgatc gagcttcccg cgacgcggca ttcggacccg
     2521 gctggccgtc gcgatcgtcg gagcggtggt ggtcctgctc gctgttgtgg ccggacccgc
     2581 cgccgccgcc ggatcgtcgc tgatccatct gagcaccgac ccctacacga attcgttctc
     2641 ggcacatcag accgaggttg agccggactc gctcgcggcc gggtcgacca tcgtgtcggc
     2701 cttccaggtt gggcgggtct tcatcggcgc ctccgccaac atcggctggg cgacttcccg
     2761 cgacggtggc aggacctggc agcacggctt cctgccaaag accacgaccc aggcctcccc
     2821 gccgggcagc tacgcccggg ccagtgacgc ctcggtcgcc tggtccgccc gcgaccacac
     2881 ctggctgatc tcctacctgg tcgctcccag cgtcacctcc aacgcgatcg tcgacctgct
     2941 ggtgagccgc tccagggacg ggatccactg gggccccccg atcacggtcg ccgcccgcaa
     3001 cgagttcctc gacaagaact ggaccacctg cgacaactcc ccggccagcc cgttctacgg
     3061 caactgctac accgagctgg aggtc
//
LOCUS       DASWDC010000148         4319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1037066, whole genome shotgun sequence.
ACCESSION   DASWDC010000148 DASWDC010000000
VERSION     DASWDC010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037066"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1258)
                     /locus_tag="VF995_04715"
     CDS             complement(137..1258)
                     /locus_tag="VF995_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342898.1"
                     /translation="MTAPPHRDANATLRKRQRSGVRLATAKATICMVSLRFGEPDGVS
                     VAAACWMTAFERLGFSVRTVAGSGTADRIVPGLALGAAEPPPLAELEAALAGADLVVV
                     ENLCSLPMNPGAALALASYLRGRRAILHHHDFPWQRGPAEGWEHWPPDDPAWLHVTIN
                     ELSRRELADRGIEAHTVYNSVEEFPPGHRGAARELLGIAPDRWLLLQPTRAIPRKNVG
                     GGIALAEALGAVYWLTGPAEDAYGPELRHLLDNARCELRRGLPPSLKIADAYAAADAV
                     LLPSIWEGFGLPLIEAALQRRPAAVGRFLVAEELAAFGFRWFPTDDPAPLRAWLEREG
                     ADPAAREVLFDHNQSIAREHFSVDALARNLGEILRQARW"
     gene            1401..2399
                     /locus_tag="VF995_04720"
     CDS             1401..2399
                     /locus_tag="VF995_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9342899.1"
                     /translation="MKIVRFVLSADPSAGVRVGYLDGVLVIPVTAAAGAGPAAGGGLD
                     TVLDLAVAANADPDLRPAPAGDPLPLGGVRLLAPVVAPPSLRDFYAFEQHVATARRRR
                     GVEMHPDWYALPVFYFSNPHAIVGPGDPVAAPPRSLELDYELEVACVLGGGGRNLGLQ
                     DADRLGLVAGFMVMNDWSARDVQRREMQQSMGPVKGKDFATSLGPWLVTTSEFAPGGL
                     REVPSAAMTARVNGVEYSRSDLDTLWWSFAEMLVYASEAAELRRGDLIGSGTCGTGCI
                     LELSIVHGLDKYPYLVPGDEVELEVEGLGVLANPIVAGDAPAFQPDPDRVRPRTAT"
     gene            2396..2899
                     /locus_tag="VF995_04725"
     CDS             2396..2899
                     /locus_tag="VF995_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9342900.1"
                     /translation="MTEPREPRIMTAEEQERQRRLTDVMWRIRHFAGAASASARTPAD
                     PQTGERWDRGQVLAHVAEMLPYWLEQAKLVAASGNGIPFGRVKSDLERVAAIERDRNE
                     NPEALLSRMDLEIDEVRAWILCLDESNWAQTGTHQTRGEMSVARIIDEFVLDHLEEHA
                     EQLEQTE"
     gene            2966..3775
                     /locus_tag="VF995_04730"
     CDS             2966..3775
                     /locus_tag="VF995_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9342901.1"
                     /translation="MNTNQQPPPPDQLVFDVTEADFESAVLQRSFQVPVVVDFWAEWC
                     APCRALGPLLERGVAARGGEVVLAKLDVDQAPELAAAFQVQGIPAVKAFSDGAIVAEF
                     VGAQPAQVVEQFLDALLPSAADRAAAEAAQLDPEQAAARWQEVVATDPGNIPARVGLA
                     QQALDRGDATEALELLKPIEYAPEATTPLAWARLAAEAANPASRFAGAARQAVDGVPE
                     PALDELLAAVREGPGTTRDHARELMLDVFHVLGDDDPRTMRYRRALSNALF"
     gene            3862..>4319
                     /locus_tag="VF995_04735"
     CDS             3862..>4319
                     /locus_tag="VF995_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX9342902.1"
                     /translation="MAKEAVLTPQGEDFPQWYQDVVARAELAENGPARGTMVIRPWAY
                     AVWELMQADMDRRIKATGVQNAYFPLFIPMSFLEKEQQHVEGFSPELAVVTHGGGEKL
                     AEPLVVRPTSETVINHYFAKWIQGHRDLPLLVNAWNNVVRWELRPRLFLRT"
BASE COUNT          616 a         1597 c         1523 g          583 t
ORIGIN      
        1 tccgactgcc gctcagcctg cggcccgagc tgctggccga gggcgttcgc cgcctcgccg
       61 gcgcctgggc ggactaccgc cgcgccctcg accgctgcga ccgcgtcgac ctcctgctct
      121 gagtgacgct gggggcctac cagcgggcct ggcggaggat ctcgccgaga ttgcgggcca
      181 gggcgtcgac cgagaagtgc tcgcgggcga tcgactggtt gtggtcgaac agcacctcgc
      241 gcgcggcggg gtcggcgccc tcccgttcca gccaggcgcg cagcggggcg gggtcgtcgg
      301 tggggaacca gcggaagccg aaggcggcca gctcctcggc gaccaggaag cggcccaccg
      361 ccgccgggcg gcgctgcagt gccgcctcga tcaagggcag gccgaatccc tcccagatgg
      421 aggggagcag caccgcgtcg gcggcggcgt aggcgtccgc gatcttgagg ctgggcggca
      481 gcccgcggcg cagctcgcaa cgggcgttgt cgagcaggtg gcgcagctcc gggccgtagg
      541 cgtcctcggc cgggccggtc agccagtaga cggcgccgag cgcctcggcc agggcgatgc
      601 cgccgccgac gttcttgcgg gggatggcac gggtcggctg cagcagcagc catcggtccg
      661 gcgcgatccc gagcagctcg cgggcggcgc cacggtgccc gggcgggaac tcctcgaccg
      721 agttgtagac ggtgtgcgcc tcgatgccgc ggtcggcgag ctcgcggcgg ctcagctcat
      781 tgatggtgac gtgcagccag gcggggtcgt cgggcggcca gtgctcccag ccctcggccg
      841 ggccgcgctg ccacgggaag tcgtggtggt gcaggatcgc gcggcggccg cgcaggtagc
      901 tggccagcgc cagggcggcg ccagggttca tcggcaggga gcagaggttc tcgaccacca
      961 ccaggtcggc gccggccagg gcggcctcca gctcggccag cggcggcggc tcggccgccc
     1021 ccagcgccag gccgggcacg atccggtcgg ccgtgcccga gcccgccacc gtgcggacgc
     1081 tgaagccgag ccgctcgaag gcggtcatcc agcaggccgc ggcgaccgac accccgtcgg
     1141 gctcaccgaa gcgtagcgac accatgcaga tggtcgcctt ggccgtcgcc agccggacac
     1201 cggaacgctg ccgcttgcgt aaggtggcgt tggcgtcccg gtgtggcggg gccgtcacgg
     1261 tcctcccgcg gggatccgct gagttgcgtt gccccacgtc atgcttttgt gatacacgac
     1321 cggcacccat ccgcgccagg gcggaattca ttgcgatttc tgcgctttgc gccaatcttg
     1381 cgtgaccaag gtggcctcct gtgaagatcg tccgcttcgt cctctccgcc gatcccagcg
     1441 ccggggtccg ggtcggctac ctggacggcg tcctggtcat ccccgtcacc gcggcggcag
     1501 gggccgggcc agcggcgggg ggcggactcg atacggtcct cgacctggcc gtggccgcca
     1561 acgccgaccc ggatctcagg cctgcgccgg ctggcgaccc gctgccactc ggcggcgtcc
     1621 gcctgctcgc ccccgtcgtc gccccgccgt cgctgcggga cttctacgca ttcgagcagc
     1681 acgtcgccac cgcccgcagg cgccgcgggg tggagatgca ccccgactgg tatgcgttgc
     1741 cggtgttcta cttctccaat ccccacgcga tcgtcgggcc gggcgacccg gtcgccgcgc
     1801 cgccccggag ccttgagctg gactacgagc tggaggtcgc ctgcgtgctc ggcggcggcg
     1861 ggcgcaacct cggcctccag gacgccgacc ggctggggct ggtcgccggg ttcatggtga
     1921 tgaacgactg gtcggccagg gacgtgcagc gtcgcgagat gcagcagtcc atgggcccgg
     1981 tcaagggcaa ggacttcgcc acctcgctcg gcccctggct ggtcaccacc agcgagttcg
     2041 cgcccggcgg cctgcgggag gtcccgagcg cggccatgac cgcgcgggtc aacggcgtcg
     2101 agtactcccg ctccgacctc gacaccctct ggtggagctt cgccgagatg ctcgtctacg
     2161 cctcggaggc ggccgagctg cggcgcggcg acctgatcgg ctccggcacc tgcggcaccg
     2221 gctgcatcct ggagctgtcg atcgtccacg gcctcgacaa gtacccctac ctggtccccg
     2281 gcgacgaggt cgagctggag gtcgagggcc tcggcgtgct cgccaacccg atcgtcgctg
     2341 gcgacgcgcc cgccttccag cccgaccccg accgggtccg accaaggacg gcgacatgac
     2401 tgaacccagg gaaccgagga tcatgacggc ggaggaacag gagcggcagc gacggctgac
     2461 cgacgtcatg tggcggatcc gtcacttcgc cggtgccgcc tccgcctcgg cgcggactcc
     2521 cgctgacccg cagaccggcg aacgctggga tcgcggccag gtcctggcgc acgtggctga
     2581 gatgctgccc tactggctgg agcaggccaa gctggtcgcc gccagcggca acggcatccc
     2641 ctttgggcgg gtcaagagcg atctggaacg cgtcgccgcg atcgagcgcg accgcaacga
     2701 gaacccggaa gcgctgctgt cgcggatgga cctggaaatc gacgaggtgc gagcctggat
     2761 actctgcctc gacgagtcca actgggccca gaccggcacc caccagaccc gcggtgagat
     2821 gtcggtggcc aggatcatcg acgagttcgt gctcgaccat ctcgaagagc acgccgaaca
     2881 gctcgaacag accgaatgac gccctgacca gcaccagcgg cgcaccagct tgcggcggag
     2941 gcagccggga gcgtaccaac atcacatgaa caccaaccag cagcccccac cgccggacca
     3001 gctcgtcttc gacgtcaccg aggccgactt cgagagcgct gtgctccagc gctccttcca
     3061 ggtccccgtg gtggtcgact tctgggcgga atggtgcgcc ccctgccggg cgctcgggcc
     3121 gctgctagag cgcggcgtcg ccgcccgagg tggcgaggtc gtgctcgcca agctcgacgt
     3181 ggaccaggcg cccgagcttg ccgccgcgtt ccaggtgcag ggcatcccgg cggtcaaggc
     3241 gttcagcgac ggcgcgatcg ttgccgagtt cgtcggcgcc cagcccgccc aggtggtcga
     3301 gcagttcctc gacgcgctgc tgccgagcgc cgccgaccgc gccgccgccg aggccgccca
     3361 gctcgacccg gagcaggccg ccgcccgctg gcaggaggtc gtcgccaccg accccggcaa
     3421 catccccgcc cgcgtcggcc tcgcccagca ggccctcgac cgcggcgacg ccaccgaggc
     3481 gctggagctg ctcaagccga tcgagtatgc cccagaggcg accaccccgc tggcctgggc
     3541 ccggctcgcc gccgaggcgg ccaaccccgc ctcccgcttc gccggcgccg cccgccaggc
     3601 cgtcgacggc gtgccggagc cggccctcga cgaactgctc gccgccgtcc gcgaaggccc
     3661 cggcaccacc cgcgaccatg cccgggagct gatgctcgac gtcttccacg tcctcggcga
     3721 cgacgatccg cgcacgatgc gctaccgccg cgcgctgagc aacgcgttgt tctaggcgcc
     3781 ggctggccgg taggctgcga gcattccgac cggaacgcgt caccgctcag tcaccgccca
     3841 ggagatccag gaggccccgc cgtggccaag gaggccgtgc tcaccccgca gggggaggac
     3901 ttcccccagt ggtaccagga cgtcgtcgct cgcgccgaac tggccgagaa cggccctgcc
     3961 cgggggacca tggtcatccg accgtgggcg tacgccgtct gggagctgat gcaggccgac
     4021 atggaccgcc gcatcaaggc gaccggcgtg cagaacgcct atttcccgct gttcatcccg
     4081 atgagcttcc tggaaaagga acagcagcac gtcgagggct tctcgcccga gctggcggtg
     4141 gtcacccacg gcggcggcga gaagctcgcc gagccgctag tggtccgccc tacctccgaa
     4201 acggtcatca accactactt cgccaaatgg atccagggcc accgcgacct gcccctgctg
     4261 gtcaacgcct ggaacaacgt ggtccgctgg gagctgcgcc cgcggctgtt cctgcgcac
//
LOCUS       DASWDC010000149         9668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1037121, whole genome shotgun sequence.
ACCESSION   DASWDC010000149 DASWDC010000000
VERSION     DASWDC010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9668
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037121"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VF995_04740"
     CDS             <1..451
                     /locus_tag="VF995_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9342903.1"
                     /translation="PGTSQEWGGIIRHGAKLLYAYAEATVPKLTVITRKAYGGAYDVM
                     ASKPLRADANFAWPTAEIAVMGPQGAVNIIFRREIEKARDQTARRDELVAEYTERFAN
                     PYIAAERGYVDEVIEPQTTRPRLVQALEMCVTKREPRPPRKHGNIPL"
     gene            448..741
                     /locus_tag="VF995_04745"
     CDS             448..741
                     /locus_tag="VF995_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342904.1"
                     /translation="MSMSGSPSVPGGSARGEPGDIVVLRTPSGLRFQLLRGEPEGDEV
                     AVLAEAIDRMAAWDEGQELGAWVRGIRPGIGRHAWQAGRRWGDSLRSGWGEEV"
     gene            742..2508
                     /locus_tag="VF995_04750"
     CDS             742..2508
                     /locus_tag="VF995_04750"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9342905.1"
                     /translation="MDKVLVANRGEIAVRVLRACRELGVTGVAVYSDADRDALHVALA
                     DEAWHIGEAAPAKSYLNIDKLVDVARRAHVDAIHPGYGFLAENAAFAQAVMDAGITWV
                     GPKPAAIATMGDKVAARRVAEQAKAPVVPGTGEPIGGPAEAVAFGKEHGWPLALKAAF
                     GGGGRGMKVVAGAKDVEEALASAQRESKSAFGRDEIYVERYLEAPRHVEAQILADGRG
                     EVVFVGERDCSLQRRHQKLVEEAPAPGLPEELRAALGKAACDIARTADYANAGTIEFL
                     YEPRTGKFYFLEMNTRLQVEHPVTELTAGVDLVHAQLRVAAGEGIPAGWDDGALAPRG
                     HAIEVRVNAEDPGQRFMPAPGPVTAWHEPSGPGVRVDAGVRAGFTVPQAYDSLLAKLI
                     VWGEDREQARRRMLRALAEFTIQGVPTTIPFHQLAMNDPAFIAGDVSTILVEHGMDLS
                     RLKPQEPIEREQTAKPPPRRLVIELEDKRFDLRVLPQEEIRLPERRRTPRSPKALARA
                     RAEMGGPGKEIVKTPMQGTIVKVLVADGDTVSPGQTIVVLEAMKMENHVTAHQAGTIS
                     GITVAEGQSVPTGATIATITAS"
     gene            2525..2959
                     /locus_tag="VF995_04755"
     CDS             2525..2959
                     /locus_tag="VF995_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342906.1"
                     /translation="MSSPDRLEAWAAANPAARDALLEVTEQAVTLQPAAEVVIQLAHD
                     DQHRAQPEWEVEQRRVEDAYRVLQARLRSLEAGGEGGQEVAKPVGALLEHHAALVAGA
                     RAAPADGLGRVAGELVAFRDRLRRTVPVRGREVADLQVDKLL"
     gene            3134..3436
                     /locus_tag="VF995_04760"
     CDS             3134..3436
                     /locus_tag="VF995_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342907.1"
                     /translation="MARFVEIVTLSPAAHQDLRGDGERFAVVKRYLELRGITMTGAFQ
                     LDGTEHLMLVLEAATPPTRTLNRALAQAWPDSGDRPGLARMVNAAPWIQRSVTYAR"
     gene            complement(3460..4047)
                     /locus_tag="VF995_04765"
     CDS             complement(3460..4047)
                     /locus_tag="VF995_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX9342908.1"
                     /translation="MRTRMGVAWWRRRGPLGAPRRPAPRLALYYDPEAFGRFSETIAR
                     YLGTARFLVVQTAVVLVWIGWNVVFRDSASGFDKYPFILLTLALSLQASYAAPLILLA
                     QNRQEERDREEMLEDRRTNVRALADTEYLARELAAIRLALGEMPTRDYLSEELERLDG
                     ELRRLRREVARAAAGGSGGLRSRAPGGNPAPAAEE"
     gene            complement(4044..5339)
                     /locus_tag="VF995_04770"
     CDS             complement(4044..5339)
                     /locus_tag="VF995_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018544343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9342909.1"
                     /translation="MAPVPAAEQLFVSRLTRLPVRTPAGGTIGRIADVVLTKPAVGSV
                     PRVLGLTVWVQRRPIFIAMGRVAELDAGGARLRTASVNLRPFELRAGETLALGELIDS
                     RVRQRETGEELRVNDAAIGQVNEEWVVTVLDVLERGSGLRPWRRARHRRLPWDEVIGV
                     EPGQSAASRLASMTDLRPADLAARLLALTPQAREEVVSELDDDRLAVALEELPEEVQA
                     ALLRRLDDERAADVLEAMDPDDAADLLGELSEADRAVLLALMQPDEAEPVRQLLVYDE
                     DTAGGLMTTEPVIMRAADTVAEALARLRDGDLPPALAGQAFVVRPPTQTPTGRYLGTA
                     HIQRLLRELPGDRLGSCIETDLDTLPPELPTRRVAEYLATYDLLGAPVCDEQGRLLGA
                     VSVDDVLDHLLPDAWRRGPIRTRSGPRPGPAGGVAEGAR"
     gene            complement(5379..8849)
                     /gene="metH"
                     /locus_tag="VF995_04775"
     CDS             complement(5379..8849)
                     /gene="metH"
                     /locus_tag="VF995_04775"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX9342910.1"
                     /translation="MERDFLNALRERVLVFDGAMGTMLHAADLSLDDYQGKEGCSEIL
                     CVTRPDVVQGIHAAYLEAGADAVETNSFGGAAIVLAEYDLADQVVELNRLAARLAREV
                     ADGYEADGRPRWVAGSMGPGTKLPTLGHTTYEVLRATYSDQAQGLLEGGADVLLVETC
                     QDLLQLKAAVAGAFAGMRRTGRRVPLMAQVTIETTGQMLLGSDIAAALTAIEPLGVDV
                     IGMNCATGPAEMGEHLRYLAQHARTPLSCLPNAGLPELRDGKTFYPLTPAELAEAHAR
                     FVAEFGLSIVGGCCGTTPEYIRQVVEAVGGHPPAHREPRHEPSLSSLYQAVTLDQDTT
                     YLTVGERCNANGSRKFRDLMLAGDLDGMTQVAKEQIREGAHVLDVCVDYVGRDGAPDM
                     DVLVETLARQSTVPVMLDSTQADVIEAGLKRLGGRAIINSINLEDGENRMATVCPMAK
                     EYGAALVALLIDEEGQARDVDWKLRVAYRIHDLAVEKYGLAPEDLVFDALTFPLGSGQ
                     EDLRGDAMATLEAIRRIKAELPGVRTILGVSNVSFGLSPAARQVLNSVFLHEAREAGL
                     DAAIVSPAKILPMHRIEEEQRQVALDLIYDRRAAGYDPLQKFMALFEGKEVAKAASAE
                     DLAALPIDERLRRRIVDGVRDGLEADLEEAMRSREPLAIVNEVLLAGMKTVGELFGAG
                     EMQLPFVLQSAEVMKAAVAVLEPYMEKADQGGKGKVVLATVKGDVHDIGKNLVDIILT
                     NNGYTVHNLGIKQPVAAIIEKAEEVGADAIGMSGLLVKSTVVMRENLEELNARGLDRY
                     PVLLGGAALNRTYVEGDLRQLYKGRVFYCRDAFAGLRVMDQLREERAAGVPVPKEPVR
                     TPRVQVEGARARGPAPVPPGTDERARSNVALDVPVPAAPFLGEARIVKGVPVREIAPY
                     LNESALFRGQWQLRPSGGKEGWQRLLETEARPRLRALLDEAITEQILRPAVVYGYFAC
                     NADGNDLIVWHGDGSDQREWVRFSFPRQRRDRRLCIADFFRPLESGERDVVAFHVVTM
                     GRAASEATARLFEADHYREYLELHGVTVELAEALAEYWHQRVREELGIAGDDAPTMEE
                     LFDQGYRGSRYSFGYPACPDLEEQSKIFELLRPERIGVELSEEYQLHPEQSTSAIIVH
                     HPEAKYFNAR"
     gene            8919..>9668
                     /locus_tag="VF995_04780"
     CDS             8919..>9668
                     /locus_tag="VF995_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX9342911.1"
                     /translation="MSERQTARVEGQPGTRDQAIPAAAATPAPAPALPPRAVLRDEVL
                     LVLFLSLAASALYALVDLLSAPLRGVTATLFSNVALIDQLLDIGTSLVPVLLVVHFLH
                     RSGESAASIGLDGREPLADSAWGAGLALVVGAVGLGIYAAAVELGVNRFVVPVPPLGH
                     WWTIPILLLAAARSGLLEEVIVCGYLLRRLDQLGWGRARALWASALLRGAYHLYQGWG
                     GFAGNLALGLFFGRLYQRRGRTMPLAVAHFLV"
BASE COUNT         1328 a         3553 c         3459 g         1328 t
ORIGIN      
        1 gcccgggacc agccaggagt ggggcgggat catccgccac ggcgccaagc tgctgtacgc
       61 ctacgccgag gcgaccgtgc cgaagctgac ggtgatcacc cgcaaggcct acggcggcgc
      121 ctacgacgtg atggcctcca agccgctgcg ggcggacgcc aacttcgcct ggccgaccgc
      181 cgagatcgcg gtgatggggc cccagggcgc ggtcaacatc atcttccgcc gcgagatcga
      241 gaaggccagg gaccagaccg cccgccgcga cgagctggtg gcggagtaca ccgagcgttt
      301 cgccaacccc tacatcgcgg ccgagcgcgg gtatgtggac gaggtgatcg agcctcagac
      361 cacccgtccc cgcctcgtcc aggcgctgga gatgtgcgtc acaaaacgag agccccgccc
      421 gccccgcaag cacgggaaca tccccctatg agcatgagcg gctcgccgag tgttccagga
      481 gggagcgcgc ggggggaacc tggggacatc gtggtcctgc gcacgccgag cggcctccgg
      541 ttccagctcc tgcgggggga gccggagggc gacgaggtgg cggtgctcgc cgaggcgatc
      601 gaccgcatgg cggcctggga cgaggggcag gaactgggag catgggtccg cggtatccgg
      661 ccggggatcg gccgacatgc ctggcaggcg ggccgccgct ggggtgacag cctgcgctcg
      721 ggctggggcg aggaggtctg agtggacaag gtgctggtgg cgaatcgggg cgagatcgcc
      781 gtgcgggtgc tgcgggcctg ccgtgagctg ggtgtcaccg gcgtcgcggt ctactcggac
      841 gccgaccggg acgccctgca cgtcgcgctg gccgacgagg cctggcatat cggcgaggcc
      901 gcgcccgcga agagctacct gaacatcgac aagctggtcg atgtggcccg ccgcgcccac
      961 gtggacgcga tccatcccgg ctacggcttc ctggccgaga acgccgcttt cgcccaggcg
     1021 gtgatggacg ccgggatcac ctgggtcggg ccgaaaccgg ccgcgatcgc caccatgggc
     1081 gacaaggtgg ccgcccgccg cgtcgccgag caggccaagg cgccggtggt gcccggcacc
     1141 ggcgagccga tcggcggccc ggccgaggcg gtcgccttcg gcaaggagca cggctggccg
     1201 ctggcgctca aggccgcctt cggcggcggc gggcgcggca tgaaggtggt cgccggcgcc
     1261 aaggatgtcg aggaggcgct cgcctccgcc cagcgcgagt ccaagtcggc cttcggccgg
     1321 gacgagatct acgtggagcg ctacctggag gcgccccgcc acgtcgaggc gcagatcctc
     1381 gccgacggcc gtggcgaggt ggtcttcgtc ggcgagcgcg actgctcgct gcagcgccgc
     1441 caccagaaac tggtcgagga ggcgcccgcg ccgggcctgc ccgaggagct ccgcgccgcg
     1501 ctcggcaagg ccgcctgcga catcgcccgc accgccgact acgccaacgc cggcacgatc
     1561 gagttcctct acgaacccag gactggcaag ttctacttcc tggagatgaa cacccggctc
     1621 caggtcgagc atccggtcac cgagctgacc gccggggtgg acctggtgca cgcgcagctc
     1681 cgcgtcgccg ccggggaggg catcccggcc ggctgggacg acggcgcgct ggccccgcgc
     1741 gggcacgcga tcgaggtccg cgtcaacgcc gaggacccgg gccagcgctt catgccggcg
     1801 cccgggccgg tcaccgcctg gcatgagccg tccgggccgg gggtccgggt cgacgccggg
     1861 gtccgcgccg gattcaccgt gccgcaggcc tacgactcgc tgctggccaa gctgatcgtc
     1921 tggggcgagg accgggagca ggcccgccgc cgcatgctgc gcgccctggc ggagttcacc
     1981 atccagggcg tccccaccac gatcccgttc caccagctgg ccatgaacga ccctgcgttc
     2041 atcgccgggg acgtctccac catcctggtc gagcacggca tggacctgtc ccggctgaag
     2101 ccgcaggagc cgatcgagcg cgagcagacc gccaagccgc ccccgcgccg cctggtgatc
     2161 gaactggagg acaagcgctt cgacctgcgg gtcctgccgc aggaggagat ccgcctgccg
     2221 gagcggcgcc gcacgccccg ctcccccaag gcgctggccc gcgcccgcgc cgagatgggc
     2281 ggccccggca aagagatcgt caagacgccg atgcagggga cgatcgtcaa ggtgctggtc
     2341 gccgacggcg acacggtcag ccccggccag accatcgtgg tgctggaggc gatgaagatg
     2401 gagaaccacg tcaccgctca ccaggccggc acgatcagcg gcatcaccgt cgccgagggc
     2461 cagtccgtcc cgaccggcgc caccatcgcc accatcaccg cgagctgaag ggcagggcgc
     2521 gtcgatgagt tcgccggatc ggctggaggc gtgggcggcg gccaacccgg ccgcgcggga
     2581 cgccctcctg gaggtcaccg agcaggcggt gacgttgcag cccgccgccg aggtggtgat
     2641 ccagctcgcc cacgacgacc agcaccgcgc ccaacccgag tgggaggtcg agcagcggcg
     2701 cgtcgaggac gcctaccggg tgctccaggc gcgactccgc tcgctggagg ccggtgggga
     2761 gggcggacag gaggtcgcca aaccggtcgg ggcgctgctg gagcaccacg cggccctggt
     2821 cgccggggcg cgcgccgctc cggcggacgg cctcggccgg gtcgccggcg agctggtggc
     2881 gttccgggac cggctgcgcc gcaccgtccc ggtgcggggc cgtgaggtcg cggacctgca
     2941 agtggacaag ctgctctaac agagcgaagt aggccaccgg agctgggcaa accgccctct
     3001 tcgggaaagg gatgatagat accctttcat cccatcgtgt gacgcacgtc acacaaaccc
     3061 tgtgaccggg atcacagccc cactccggcg gatgggcttc gatgtaggag cagcaagcca
     3121 gctggaggct gggatggcaa ggttcgttga gatcgtgacg ctttcccctg ccgcgcacca
     3181 agatctacgc ggggacgggg agcgcttcgc cgtagtcaag cgttacctgg agctgcgcgg
     3241 catcacgatg acgggagcct ttcagctcga cgggaccgag cacctgatgc tggtccttga
     3301 ggccgccacg cctccgaccc gaaccctgaa tcgggcgctg gctcaggctt ggccggactc
     3361 gggggaccgg ccgggcctgg cccggatggt caacgccgcc ccgtggatcc agcggtccgt
     3421 tacctacgcc cgctgactcc atcggaggcc acctcaccat cactcctccg ccgccggggc
     3481 ggggttgcct ccgggtgccc tggatctgag ccccccggac cccccggccg ccgccctggc
     3541 cacctcgcgg cgcagccgcc gcagttcgcc gtcgagccgt tccagctcct cgctgaggta
     3601 gtcgcgggtg ggcatctcgc cgagggcgag ccggatcgcg gccagctcgc gggccaggta
     3661 ctcggtgtcg gccagggcgc gcacgttggt gcgccggtcc tcaagcatct cctcgcggtc
     3721 gcgctcctcc tggcggttct gcgccagcag gatcagcggc gcggcgtagg aggcctgcag
     3781 cgacagggcc agcgtcagca ggatgaacgg gtacttgtcg aacccggaag ccgagtcgcg
     3841 gaacaccacg ttccagccga tccacaccag caccaccgcc gtctggacca ccaggaagcg
     3901 tgcggtgccg agataccggg cgatcgtctc ggagaagcgc ccgaacgcct ccgggtcgta
     3961 gtacagcgcc agcctcggcg ccggccgccg cggcgccccg agcggccccc gccggcgcca
     4021 ccacgccacc cccatccggg tcctcatctc gccccctccg cgacgccgcc ggccgggccg
     4081 ggccgaggtc cgctgcgggt gcggatcggc ccccggcgcc aggcgtcggg cagcaggtgg
     4141 tcgagcacgt cgtcgaccga gaccgcgccg agcaggcggc cctgctcgtc gcagaccggc
     4201 gcgcccagca ggtcgtaggt ggccaggtac tccgcgaccc gccgggtcgg cagctccggc
     4261 ggcagcgtgt ccaggtcggt ctcgatgcag gacccgagcc ggtcgccggg cagctcccgc
     4321 agcagccgct ggatgtgggc ggtgccgagg tagcggccgg tcggggtctg ggtgggcggg
     4381 cgcaccacga acgcctgccc ggccagcgcg ggcggcaggt ccccgtcgcg cagccgcgcc
     4441 agcgcctcgg cgacggtgtc ggcggcgcgc atgatcaccg gctcggtggt catcagcccg
     4501 cccgcggtgt cctcgtcgta gaccagcagc tggcggaccg gctcggcctc gtcgggctgc
     4561 atcagcgcca gcagcaccgc ccggtcggcc tcggacagct cgccgagcag gtcggcggcg
     4621 tcgtcgggat ccatcgcctc cagcacgtcg gcggcgcgct cgtcgtccag gcgccgcagc
     4681 agcgccgcct ggacctcctc gggcagctcc tccagcgcca ccgccagacg gtcgtcgtcc
     4741 agctcgctca ccacctcctc gcgggcctgc ggggtcaggg cgagcagccg cgcggccagg
     4801 tcggcgggcc gcaggtcggt catggacgcc agccggctcg cggccgactg gcccggctcc
     4861 accccgatca cctcgtccca cggcaggcgc cggtggcgcg cgcgccgcca gggccgcagt
     4921 cccgacccgc gctccagcac gtccaggacg gtgaccaccc actcctcgtt gacctggccg
     4981 atggcggcgt cgttgacgcg cagctcctcg ccggtctcgc gctggcggac gcgggagtcg
     5041 atcagctccc cgagcgccag cgtctccccg gcgcgcagct cgaacggccg caggttcacg
     5101 ctggcggtcc gcagcctggc gccgcccgcg tccagctccg cgacccggcc catggcgatg
     5161 aagatcggcc ggcgctgcac ccacacggtc aggccgagca cccgcggcac cgacccgacc
     5221 gccggcttgg tcagcaccac gtcggcgatc cggccgatcg tcccgcccgc cggggtccgt
     5281 accggcagcc gggtgaggcg cgacacgaac agctgctcag ccgctggaac cggggccacg
     5341 ccacacctcc ccagcgcgct caggctctcg gcggagcctc agcgcgcgtt gaagtacttc
     5401 gcctccggat gatgcacgat gatcgccgag gtggactgct caggatgcag ctggtactcc
     5461 tccgagagtt cgacaccgat ccgctcgggc ctgagcagct cgaagatctt gctctgctcc
     5521 tccagatcgg ggcaggccgg gtagccgaac gaatagcgcg aaccccggta gccctggtcg
     5581 aacagctcct ccatggtggg cgcgtcgtcg ccggcgatcc ccagctcctc gcggacgcgc
     5641 tggtgccagt actcggccag cgcctcggcc agctccacgg tgacgccgtg cagctccagg
     5701 tactcgcggt agtggtcggc ctcgaacaac ctggcggtcg cctccgaggc cgcccggccc
     5761 atcgtcacga cgtgaaacgc gaccacgtcc cgctcaccgg actcgagcgg ccggaagaag
     5821 tcggcgatgc acagccgccg gtcgcgccgc tggcggggga aactgaagcg gacccactcc
     5881 cgctggtcgc tgccgtcgcc gtgccagacg atcaggtcgt tgccgtcggc gttgcaggcg
     5941 aagtagccgt agacgaccgc cgggcgcagg atctgctcgg tgatcgcctc gtcgagcagg
     6001 gcccgcagcc gcggcctggc ctcggtctcc agcagccgct gccagccctc cttgccgccg
     6061 ctcgggcgca gctgccactg gccccggaac agcgccgact cgttcaggta cggagcgatc
     6121 tcgcgcaccg ggacgccctt gacgatccgc gcctccccca ggaacggcgc ggccgggacc
     6181 ggcacgtcca gcgccacgtt ggagcgggca cgctcgtcgg tgccgggcgg gaccggcgcg
     6241 gggccgcgag cgcgggcgcc ctccacctgg actctgggcg tgcggaccgg ctccttgggc
     6301 accgggaccc ccgccgcgcg ctcctcgcgg agctggtcca tcacccgcag gccggcgaag
     6361 gcgtcgcggc agtagaagac ccggcccttg tagagctggc gcaggtcccc ctcgacgtag
     6421 gtgcggttga gggcggcgcc gccgagcagc accgggtagc ggtccagccc gcgggcgttg
     6481 agctcctcca ggttctcgcg catcaccacg gtcgacttca ccagcaggcc ggacatgccg
     6541 atcgcgtcgg cgccgacctc ctcggccttc tcgatgatcg cggcgaccgg ctgcttgatg
     6601 ccgaggttgt ggaccgtgta gccgttgttg gtgaggatga tgtcgaccag gttcttgccg
     6661 atgtcgtgca cgtcgccctt gacggtggcg agcaccacct tgcccttgcc gccctggtcg
     6721 gccttttcca tgtagggctc caggaccgcc acggcggcct tcatcacctc ggcggactgc
     6781 agcacgaagg ggagctgcat ctccccggcg ccgaacagct ccccgacggt cttcatcccc
     6841 gccagcagca cctcgttgac gatcgccagc ggctcccggg agcgcatcgc ctcctccagg
     6901 tccgcctcca gcccgtcgcg cacgccgtcg acgatccgcc gccgcagccg ctcgtcgatc
     6961 ggaagcgcgg cgaggtcctc ggcgctggcg gccttggcga cctccttgcc ctcgaacagg
     7021 gccatgaact tctgcagcgg gtcgtagcct gccgcccttc ggtcgtagat caggtcgagg
     7081 gcgacctggc gctgctcctc ctcgatgcgg tgcatcggca ggatcttggc gggcgagacg
     7141 atcgccgcgt ccaggcccgc ctcgcgcgcc tcgtgcagga acaccgagtt gagcacctgc
     7201 cgggccgccg gggagaggcc gaagctgacg ttggagactc cgaggatcgt gcggaccccg
     7261 ggcagttccg ccttgatgcg gcggatcgct tcgagggtgg ccatggcgtc gccgcgcagg
     7321 tcctcctgcc cggagccgag cgggaaggtg agcgcgtcga acaccaggtc ctcgggcgcc
     7381 aggccgtatt tctccaccgc caggtcgtgg atgcggtagg cgacccgcag cttccagtcg
     7441 acgtcgcggg cctggccttc ctcgtcgatc agcagggcga ccagggcggc gccgtactcc
     7501 ttggccatcg ggcagacggt ggccatgcgg ttctcgccgt cctccaggtt gatcgagttg
     7561 atgatcgccc ggccgccgag ccgcttcagg cccgcctcga tcacgtcggc ctgggtcgag
     7621 tcgagcatga ccggcacggt cgactgccgc gccagcgtct cgaccagcac gtccatgtcc
     7681 ggcgcgccgt cgcggccgac gtagtcgacg cagacgtcca gcacgtgggc gccctcgcgg
     7741 atctgctcct tggcgacctg ggtcatgccg tccaggtcgc cggccagcat caggtcgcgg
     7801 aacttgcgcg agccgttggc gttgcagcgc tcccccaccg tcaggtaggt cgtgtcctgg
     7861 tcgagggtga ccgcctggta gaggctcgac aggctcggct cgtgccgcgg ttcccggtgc
     7921 gccggcgggt ggccgccaac cgcctcgacc acctgccgga tgtattccgg ggtggtcccg
     7981 cagcagccgc cgacgatgct gaggccgaac tcggcgacga agcgggcgtg cgcctcggcc
     8041 agctccgccg gggtcagcgg atagaaggtc ttgccgtcgc gcagctccgg caggccggcg
     8101 ttcggcaggc acgacagggg agtccgcgcg tgctgggcga ggtagcgcag gtgctcgccc
     8161 atctcggccg ggccggtggc gcagttcatc ccgatcacgt ccacgccgag cggctcgatc
     8221 gcggtcagcg ccgccgcgat gtcggagccg agcagcatct gcccggtcgt ctcgatggtc
     8281 acctgcgcca tcagcggcac ccggcggccg gtccgccgca tcccggcgaa cgcgcccgcc
     8341 acggcggcct tcagctgcag caggtcctgg caggtctcca ccagcagcac gtcggcgccg
     8401 ccctccagca gcccctgcgc ctggtcggag taggtcgccc gcaggacctc gtaggtggtg
     8461 tgcccgaggg tcggcagctt ggtcccgggt cccatcgacc cggccaccca gcggggccgc
     8521 ccgtcggcct cgtacccgtc ggccacctcc ctggccagcc gggcggccaa gcggttcagc
     8581 tcgaccacct ggtcggcgag gtcgtattcg gccagcacga tcgcggcgcc accgaacgag
     8641 ttggtctcga ccgcgtcggc gccggcttcg aggtaggcgg catggattcc ctggaccacg
     8701 tccggccggg tcacgcagag gatctcgctg cagccctctt tgccctggta gtcgtccagc
     8761 gagaggtcgg cggcatgcag catcgtgccc atggccccgt cgaagacgag cacccgctcc
     8821 cgcagcgcat tgaggaagtc tcgttccatg cccgaaaggg tagacgaaat cccccgaggg
     8881 catccgccaa gccaggctga gcgaagtacg gtattgagat gagcgagcgt cagacagccc
     8941 gtgtggaggg ccagcccggg acgcgggacc aggcgatccc ggccgccgcg gcgaccccgg
     9001 ccccggcccc ggcgctgccg ccgcgtgcgg tgctgcgcga cgaggtgctg ctggtcctgt
     9061 tcctctcgct ggcggcttcg gcgctgtatg ccctggttga tctgctgtcg gcgcccctgc
     9121 gcggcgtcac cgcgacgctg ttcagcaacg ttgcgctgat cgaccagctg ctcgacatcg
     9181 gcacctcgct ggtgccggtg ctgctggtgg tccacttcct gcaccgctcg ggcgagtcgg
     9241 ccgcgagcat cggcctggac ggccgcgagc cgctcgccga cagcgcctgg ggcgcgggcc
     9301 tggcgctggt ggtcggcgcg gtcgggctgg gcatctacgc ggcggcggtg gagctgggcg
     9361 tgaaccgctt cgtggtgccg gtcccgccgc tcgggcactg gtggacgatc ccgatcctgc
     9421 tgctggccgc cgcccgcagc ggcctgctgg aagaggtgat cgtctgcggc tacctgctgc
     9481 gccgcctcga ccagctcggc tgggggcggg cgcgggcact gtgggcgagc gccctgctgc
     9541 gcggcgccta ccacctctac cagggctggg gcgggttcgc cggcaacctc gccctgggcc
     9601 tgttcttcgg ccgcctgtac cagcgccggg gccggacgat gccgctggcg gtcgcccact
     9661 tcctggtc
//
LOCUS       DASWDC010000150         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_435498, whole genome shotgun sequence.
ACCESSION   DASWDC010000150 DASWDC010000000
VERSION     DASWDC010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435498"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            834..2054
                     /locus_tag="VF995_04785"
     CDS             834..2054
                     /locus_tag="VF995_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018809847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX9342912.1"
                     /translation="MLAFRRQHHQEIATVLGGLDATEPRITELYTKAVEQLSSDQAPV
                     RFGGLHALERLAQDNRAHRQTIVDVICAYLRVPFSPTAPASKPEPEAAGGQEEPGTES
                     EMETDGIGGTWQQEREVRLTAQRILAEHLRDDRAKDQQSTDPPNSRFWDNIRLDLTGA
                     TLIDFNLVNGVMADANFHRAAFSGDAWFGGATFSGTARFGGAAFSGDALFGGAAFSAA
                     ANFPEAAFSGDALFGGATFSRGAVFRRAAFGGGAVFRKAIFGGGAAFGEAAFGGDAWF
                     DKATFSRGASFDEAAFTGDAWFGEAAFTGVAGFGEAAFGGDAWFDKATFSRGASFDEA
                     AFTGDAGFGEAAFTGGAWFGEATFSAGADAVHLEKARILSPGAAHVWPTGWRLADADG
                     GGYTVVRANDDGGS"
     gene            complement(2259..>2806)
                     /locus_tag="VF995_04790"
     CDS             complement(2259..>2806)
                     /locus_tag="VF995_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342913.1"
                     /translation="VGGGSSDITRLRKLGNRLLAEVVNLCHGTDYSDLCYGFNVFWQK
                     YVPVLDLDITSPPSPRGDGLLWGDGFEIETLIHMRVAEEGLRVAEVPSFEHPRIHGVS
                     NLDAFSDGLRVLTTILVERRRARRRHVSRHRASGARTPAVLARDLIHMETALVRMLYV
                     LHSVDHGRPRTLRSQVPPPPR"
BASE COUNT          476 a          914 c          956 g          460 t
ORIGIN      
        1 gaggtggaga tcatggataa atccgacgga tcgcccgggg aacgcatcgt cgaatggaat
       61 ctgtcctatt gtcttgggga cctgctctag tggttgtgca cccatcactc acctctcagc
      121 agccgggtac cggtactgtg ctcttctgag gggacccgtc aatcggtctc catgatgtca
      181 ccgcgggcaa tttcagctgc cgaatttcgg gccgaaccga gacgacgacc ctgatacttg
      241 gccagcacca ccatcgcgga ccgagggatt gccgccccag tgcccctgcg ccgtcggcac
      301 ccctcgagaa gtgagtcgtt gtgatcggcg cagacgatga tctccatcgg gatccgactc
      361 tggtgatgac accgacccct ggagaagcgg ccaacgatcc tccgtggacg tacagatgac
      421 gacggatacg gtcaaagacg tccgctccat cggcagcggg tgtggctctc cggcagcagt
      481 gagcgacgga ggcaatagca acgctggctc ctcccctctc gccccgccca taccactcgt
      541 caacgtagga cagcacgggc ccctccccaa cattctacga cgcccgcaac ggccgttcca
      601 agtgggcccg ggcagtccct ggcgtcggga atggcaggcc aaggcacggc tagaggtccg
      661 gcgaccgctg gtagcggcgg cgcgtgaagg cggcacgggc ggccgcaggt ccaggctggc
      721 tgcccgcgag gacgtcatgg ccgcgagatg atgtccccat gaagctcccc catggccgat
      781 gggcacatga gggtgccagg ccagcgtggc gagcgaggtc ggcctggctg tggatgcttg
      841 ccttccgccg ccagcaccac caggagatcg cgacagttct gggcggcctt gacgcgaccg
      901 agccccgcat caccgagcta tacaccaagg cggttgagca gctcagcagc gaccaggccc
      961 cggtccggtt cggcggcttg cacgcactcg aacggctcgc tcaggacaac cgtgcacacc
     1021 gccagaccat cgtcgacgtc atttgcgcct acttgcgcgt gccgttctcc cccacggcgc
     1081 cagccagcaa gccggagccc gaggccgccg gaggccagga agagcctggc actgagagtg
     1141 aaatggagac tgatgggatt ggcggcacgt ggcagcaaga aagagaggtt cgtcttaccg
     1201 cacagcgtat tcttgccgag catctgcgtg atgacagggc caaagatcag cagtccactg
     1261 atcctccgaa ctcacgtttc tgggataaca tccgcctcga tctcactggc gccactctca
     1321 tcgatttcaa cctcgtaaac ggcgtaatgg ccgacgccaa cttccacagg gccgccttca
     1381 gcggcgacgc ctggttcggc ggggcgacct tcagcggcac cgcccggttc ggcggtgcgg
     1441 ccttcagcgg cgacgcgctg ttcggcgggg cggccttcag cgccgccgcc aacttccctg
     1501 aggcggcctt cagcggcgat gcgctgttcg gcggggcgac gttcagccgc ggcgccgtgt
     1561 tccgcagggc ggccttcggc ggcggcgccg tgttccgcaa ggcgatcttc ggcggcggcg
     1621 ccgcgttcgg cgaggcggcc ttcggcggcg acgcgtggtt cgataaggcg acgttcagcc
     1681 gcggcgcctc gttcgacgag gcggccttca ccggcgacgc gtggttcggc gaggcggcct
     1741 tcactggcgt cgctgggttc ggcgaggcgg ccttcggcgg cgacgcgtgg ttcgataagg
     1801 cgacgttcag ccgcggcgcc tcgttcgacg aagcggcctt caccggtgac gccgggttcg
     1861 gcgaagcggc cttcaccggc ggcgcctggt tcggcgaggc gactttcagc gccggcgctg
     1921 atgcagtgca tctcgaaaag gcgcgaatcc tttcacccgg cgccgcgcac gtgtggccaa
     1981 caggatggcg cctcgctgac gccgacggcg gtggatacac ggttgtccgc gcgaacgacg
     2041 acggcggttc ttgagctgcg gggccgactt gcgccgctga agaggtggcg ttggccttcc
     2101 tcacgctgcc aggatcatca cggccgggca cggcgcgact gcgcggggaa ggcgacgggg
     2161 gtagcccggc ccgggcgcag gtcatgaccg ccgtctgcct ttgtcatcca ctccaccaca
     2221 gcacagcgga gaccggccgg gcagggccga tgcgaccttc agcggggagg tggcgggacc
     2281 tgcgaccgta gtgtgcgggg gcggccgtgg tcgacggagt gcaggacgta aagcatacgc
     2341 acgagggccg tctccatgtg gataaggtcg cgggccagta ccgctggggt tcgtgcgccc
     2401 gatgcacgat gacgggaaac gtgacggcgg cgggcgcggc ggcgttcgac caggatggtc
     2461 gtcagcacgc gaaggccatc gctgaaggcg tccaggttgc tcaccccgtg gatcctggga
     2521 tgctcgaaac ttggaacttc agcgacgcgg agtccttcct ccgctacccg catatggatc
     2581 agcgtttcga tctcgaagcc gtcgccccac aacagcccgt ctcccctggg tgaaggggga
     2641 gaggtgatgt cgaggtccaa taccggaacg tacttctgcc agaacacgtt gaatccgtaa
     2701 cacaggtcgg agtagtccgt tccgtggcag agattgacca cctcagcgag caggcggtta
     2761 ccgagcttgc gcaggcgcgt gatgtcactg ctgccgccgc ccacgg
//
LOCUS       DASWDC010000151        12565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1204973, whole genome shotgun sequence.
ACCESSION   DASWDC010000151 DASWDC010000000
VERSION     DASWDC010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12565
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204973"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..1366
                     /locus_tag="VF995_04795"
     CDS             374..1366
                     /locus_tag="VF995_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342914.1"
                     /translation="MSNEGFKRNTPGGQPNVPLEEMPTQEQAQPDLGQQPEWAAMLNQ
                     AMPQPAGQRVVEVRPPRRRVGMLVAAGFGIVGLVVGAALMRPAFTRSSINATTVGAVA
                     TTAGGTAQAQGSGQGGASAPAASNGNGNGNGNVQIVRQGFAQLPAEADGGQKLTYAVV
                     LRNPRSNQVAADVHAIISFTSRNGVAIEPEDKDLNALLPGQTSAVTGDVDGAGVTQMS
                     VRVVVTRWAPAQGLSGSLTASGINSNRVADELTTTASVHSTLARNLSDVKVTAVYYNG
                     AGQIIGGDDDSIDFLPANGTAPVTIDSSHVPMGVARTAVYANPDGLFTVPDSSD"
     gene            complement(1484..2095)
                     /locus_tag="VF995_04800"
     CDS             complement(1484..2095)
                     /locus_tag="VF995_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9342915.1"
                     /translation="MSTDLHAHDRQLIRELVENWVVWRDAGDWERFRGVWHDDGRMMA
                     TWFQGPFEEFIKVSREGWERGVSILHFLGGSSVDVAGDRALSQTKMTISQRGPVHGVL
                     CDVVCTGRFYDFIERRDGRWGLVLRQPIYEKDRLDPVDPAATLALDAARLAAFPSGYR
                     HLAYLQVEAGYAVKPDMPGLTGPEVETLYARGAAWLAGGPAGG"
     gene            complement(2136..2918)
                     /locus_tag="VF995_04805"
     CDS             complement(2136..2918)
                     /locus_tag="VF995_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9342916.1"
                     /translation="MSDEVRGGVQSVTRALQALELIAEAGELGVTDLGRRLGVHKATA
                     SRLAATLAGGGLVERDPVSDRYRLGFGLIRLAGTAMAGLDLVRTGRPVLEELADRTRE
                     SVTLGVLSNDEVIYVDQVASPRLIVSGNWIGQRTPLHCSASGKVFLAWLSPSKRDQLL
                     ARPLQVYTPQTVTDPASLRQQLEEARIRGYACIQDEIEEGLNAVAAPVRQANGEVVAA
                     IAVSGPSFRVRAIDLPGLGRVTVDAAVAMSRRLGHAAHRGPQ"
     gene            2971..4170
                     /locus_tag="VF995_04810"
     CDS             2971..4170
                     /locus_tag="VF995_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEX9342917.1"
                     /translation="MAMRYWRAAEPKRSYDVVVVGGGGHGLATAYYLARNHDLTDVCV
                     VEKGWLGGGNTARNTTIIRSNYLWDESAAIYEHSLKLWERLEEDLGLDLRFSQRGVLN
                     LAHDLGEMRSSLRRVNANALNGVDAEWLTPAEVAAFCPVVNVSPDVRYPVLGATLQRR
                     GGIARHDRVAWGFASVADRLGVDIVEHCEVTGFVRSPAGRVTGVETTRGTITANKVAV
                     VAAGHTSVLAAMVGLRLPLQSHPLQALVSVLLTPVLDCVVMSNAVHVYVSQADKGELV
                     MGAGIDGHNSYTQRGAFHLIEEQLAAALELFPVFAHAKLLRTWGGIVDVCPDASPIIG
                     PTPIEGLWLNCGWGTGGFKATPGSGWVFAETIAKGEPAPLALPFALDRFTTGALINEH
                     GAAAVAH"
     gene            4184..4435
                     /locus_tag="VF995_04815"
     CDS             4184..4435
                     /locus_tag="VF995_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX9342918.1"
                     /translation="MLLLPCPWCGLREEVEFRYGGQAGIAYPADPDALSDEEWADYLF
                     MRDNPKGPFAERWYHVHGCRRWFNLVRDTATNELLAEGR"
     gene            4437..7181
                     /locus_tag="VF995_04820"
     CDS             4437..7181
                     /locus_tag="VF995_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017623825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9342919.1"
                     /translation="MPGPGPGRRLASGGAIDRTRTLGFTFDGREYAGLEGDTLASALL
                     ANGLAGGFQSPLLGRPRGVMSAGPEEPCAFVEVSAPWFDLLAPATMLRLVDGLAAIPR
                     TGVGRLPPPDASAVAARPRHAHVETLVVGGGVAGLRAAREAAERGDRVLLLDERSWLG
                     GTARSFEQVEGRPAWEWVEETAAALRSAPDVTLLTEATALGLYDAGYAVIHERSRPVE
                     HLWHVRAKRVVLATGAHERPVAFAGNDRPGVMLSGAVLTYLDRFGVLPAERAVVWVAN
                     DAGYATARALRAAGAEVVAVVDARREGEAAAQDQARADGLEVLAGSVIAATDAATGSG
                     HVSGAFAVGPDGQRIRLEAELLAVSGGWNPVVQLHRAIGGGLRYDPARACFVPDGGPT
                     WLSATGAAAGEVPAAEPVWHIAAGDGDSSALSTSLSEHFVDLQRDQTVSDVAGALDAG
                     LRSVEHIKRVTHIGTAADQGRTSGTLAAEIVNQLLGADPGAQGPTGARPPWAPLPYHV
                     LAGPFRGRLFDPVRETPIHPWHVAHGAVFENVGQWKRPWYFQREGESMEEAVLRECAA
                     ARDGVALMDASTLGKIEVVGPDAATFLDRMYTNAISTLPVGRLRYGVMLGLDGMVFDD
                     GVAMRLAADRYLVTTTTGNAAAVLDRFEEWLQTEWPELRCWATSVTEQWATIAVVGPL
                     AREVLWAAGTDVDVSTAAFPFMAWRDGTVAGVPARVTRVSFSGSLAFEVNIASWHGLQ
                     VWEALYAAGQPFGLTVYGTETMHVLRAEKAFPIVGQDTDGTVTADDLGMSWIVNLRKG
                     DFVGRRSLRRADLTRPNRKQLVGLLPQDTAALIPEGAQLIDPGSVGASPPVPMRGHVT
                     SSYRSAALGRTFALALLEAGRQRHGDTVFAFDARLTEPIAATVADPVFYDPEGARRDG
                     "
     gene            7174..7671
                     /locus_tag="VF995_04825"
     CDS             7174..7671
                     /locus_tag="VF995_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEX9342920.1"
                     /translation="MADVSMVEVPTLSQVSLRVDLTPAQGRPAPPLELPLQPNNWRRI
                     GILEALWLGPDEWLVTAGQGNTGKMVILLEAAFDGKPHSVVDVSANRIAIDLTGPDRL
                     DLLATGCPLDLHPRVWNEGACAQTLLARVPVLLQERAAGTRLFVRPSFAAWLTDWLDA
                     AAAVS"
     gene            complement(7675..9219)
                     /locus_tag="VF995_04830"
     CDS             complement(7675..9219)
                     /locus_tag="VF995_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019812318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX9342921.1"
                     /translation="MSNQAEPRGRRSGGRGGRRAARLHAVVERVPFITRTMAPFEVLS
                     EEGLALIEHNADVILEQVGVEFRDADALATLKDAGAEVDGELVRFPRGMARALVQATA
                     PREFTQHARNPANDVRIGGDATVFAPAYGPPFVRDLDQGRRYGTIEDFRNFVKLTYAS
                     PWLHHSGGTVCEPVDLPVNKRHFDMVEAHLRLSDKPFMGSVTHPDRASDTVAMARIVF
                     GAERLERDCVILSLINANSPLVWDATMLGAARVYAEANQATLMTPFILAGAMSPATVA
                     GTAAQTLAEALAGMAYVQLVRPGAPVVFGSFASSMSMQSGAPTFGTPEPALVLYALAA
                     CARRLGVPFRSGGALTAAKVPDAQAAFESANTMQPTMLGGVNFVLHVAGWLEGGLCMG
                     YEKFVLDNDQAGAWHAFARGVDLSPNGQALDAIIANGPGQHFLGSAHTLANFETAFWR
                     SQVADANSFEQWDLEGSRDAAQRANALWKKTLADYQPPPIDDAVDQELREWIERRKAS
                     FPDSNV"
     gene            complement(9216..11159)
                     /locus_tag="VF995_04835"
     CDS             complement(9216..11159)
                     /locus_tag="VF995_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX9342922.1"
                     /translation="MTSVLEAARALGVDLDSVCGGRGICGRCQIRVSEGAHAKLQIES
                     RADHLTPWAAPEATYRARRGLDPERRLGCHARMVGDLVIDVPPDSQLHRQVVRKPAEA
                     RAIELDPVVRLCYVEVEQPALGTQTSDHARLLEALEREWGLTGLTADIHVVQGLQQAL
                     RKGEWAVTVAVHDGSAITAVWPGFRDVALGIAFDVGSTTVAGHLCDLATGEVLASAGA
                     MNPQIRFGEDLMSRVSYVMLNPGSTAELTRAVRGCLNDLVVALVAEAGREPGDVLEVT
                     LVGNPVMHHLALGIDPTELGGAPFALATGQALRLHAATELELGVHPGARAYVLPCIAG
                     HVGADAAGVLLAEAPHQRDDLSLLVDVGTNAEIVLGNRDRLLAASSPTGPAFEGAQIS
                     AGQRAAPGAIERVRIDRDTLEPRLRVIGNPAWSDQPGFDPAAVSGICGSGIIEAVAEL
                     YLAGVVTAAGVIDGAAAARSSRVVPDGRTYAYVLHDRALQRILVTQNDIRQIQLAKAA
                     LHAGCRLLMDRLGVDQVDRVRLAGAFGAHIDPVHALVLGMVPDCDPAAVSSAGNAAGT
                     GARIALLSGRSRREVEEAVRRVEKVETAVEPRFQEHFVDAMAIPHRRDPYARLAKVVT
                     LPERDLDANGRMHPRTRTRRRTR"
     gene            complement(11213..12139)
                     /locus_tag="VF995_04840"
     CDS             complement(11213..12139)
                     /locus_tag="VF995_04840"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007235347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9342923.1"
                     /translation="MTHTVVSSASKEVVIGFDRPFVIIGERINPTGRKRLAAEMAIGD
                     FSTVIADALAQVEAGAHMLDVNAGVPLADEPAILTRVVELVQSVTDVPLCIDSSIVEA
                     LEAALPVYKGKALVNSVTGEEEQLQRVLSLVARHGAAVVAISNDERGISENPDVRFEV
                     ARKIVERAADYGITPADIVVDPLVMPIGAVASAGRQVFHLVRRLRQELHVNTTCGASN
                     IAFGLPNRHALSAAFLTMAAAAGMTSAITNPLHEDVRRAVAAADVLLGNDRDCMTWIS
                     RYRDGAQPGTSDGGAGRRRGGREGRRRAAQDL"
     gene            complement(12136..>12565)
                     /locus_tag="VF995_04845"
     CDS             complement(12136..>12565)
                     /locus_tag="VF995_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEX9342924.1"
                     /translation="ILVAYGDCGTGGALDRVLDEHGVGRLPGDHCYAFFAGVDSWLAL
                     HDAEPRTFYLTDFLCRHFDRLVVRGLGLDRHPELLPAYFGNYVRLVYLAQRDDEALLA
                     KAKEAAAYLGLTLEVRHSGYGDLVPALQAARQRAVEVACA"
BASE COUNT         1766 a         4480 c         4607 g         1712 t
ORIGIN      
        1 agcctcggca ggtgcgcccc ccgcctggcg tcccccatcg cgctcgccac cgccagccac
       61 ccaagcccag ctgccagcaa cccatccacc accggcaccg gcagccctcg cacccacccc
      121 ggcacccgcc agccccgcgc caccctcaca ccaaacccca caaacaccgc acccgcgctc
      181 ctcgtcgagc gccatcatct cggaaccggg cccgtggcac cacacctagg gtggtggagg
      241 caacacccgg cgggggtggg ggccacccct tgaggcccta ctgggcgggg gtcactgggc
      301 gcagcatggg tgcagcggac caagaccaag aggaccaggg cgataaggcc cagtagttgg
      361 agggggaagg gtcatgagca acgagggatt caagcggaac acgccgggcg ggcagccgaa
      421 cgtgccgctg gaggaaatgc cgacgcagga gcaggcgcag ccggacctgg gacagcagcc
      481 ggagtgggcg gcgatgctga accaggcgat gccgcagccg gccgggcagc gggtggtcga
      541 ggtgcggccg ccgcggcggc gggtcgggat gctcgtggct gctggcttcg ggatcgtcgg
      601 gctggtggtg ggggcggcgc tgatgcggcc ggcgttcacc cgcagctcga tcaacgcgac
      661 cacggttggc gctgtcgcca cgactgcggg cgggaccgcc caggcccagg gctccgggca
      721 gggcggcgcg tcggcgccgg ccgccagcaa cggcaacggc aacggcaacg gcaacgtcca
      781 gatcgtgcgt cagggcttcg cgcaactccc ggccgaggcg gacgggggcc agaagctgac
      841 ctacgcggtg gtcctgcgca acccgcgcag caaccaggtc gccgcggacg tgcacgcgat
      901 catcagcttc accagccgca acggcgtggc gatcgagcct gaggacaagg acctgaacgc
      961 gctgctgccg gggcagacca gcgcagtgac cggcgacgtc gacggtgccg gcgtgaccca
     1021 gatgagcgtc cgggtggtcg tcacccgctg ggcgccagcc cagggcctca gcggcagcct
     1081 gaccgccagc ggcatcaaca gcaaccgggt cgccgacgag ctgaccacca cggcgagcgt
     1141 ccactcgacg ctggcgcgga acctcagcga cgttaaggtg accgcggtct actacaacgg
     1201 cgctgggcag atcatcggcg gcgacgacga cagcatcgac ttcctgcccg ccaacgggac
     1261 ggctcccgtg acgatcgaca gctcccacgt gccgatgggc gtcgcccgca ccgcggtcta
     1321 cgccaacccc gacggcctct tcacggtccc ggactcctcg gactgaaccc cgcactccga
     1381 gcccggacca gccggggtcg cggcgcccag cagccaggcg ccgcggcccc ggccttttgg
     1441 cgtcgcattg gcgttcgcat tggcgtcgcc gctgcgggtg cgctcagccg ccagccggac
     1501 cgccagcgag ccaggcggcg ccgcgggcgt agagggtctc gacctctggg ccggtgaggc
     1561 cgggcatgtc cggcttgacg gcgtagccgg cctcgacctg caggtaggcg aggtggcggt
     1621 agccggaggg gaaggcggcc agccgcgccg cgtcgagggc cagagtcgcc gccgggtcaa
     1681 ccgggtcgag gcggtccttc tcgtagatcg gctggcgcag gaccagcccc cagcgcccgt
     1741 cgcgccgctc gatgaagtcg tagaagcggc cggtgcagac cacgtcgcag agcacaccgt
     1801 ggaccgggcc gcgctgggag atggtcatct tggtctggga gagcgcccgg tccccagcaa
     1861 catcgaccga gctgccgccg aggaagtgca ggatgctcac cccgcgctcc cacccttcgc
     1921 ggctgacctt gatgaactcc tcgaagggac cctggaacca ggtggccatc atgcgcccgt
     1981 cgtcgtgcca gacgccgcgg aaccgttccc aatccccggc gtcgcgccac accacccagt
     2041 tttcgaccag ctcgcggatg agctgacggt cgtgcgcgtg caggtccgtg ctcacgatca
     2101 cctcctgtga cggtccctgg tgggggcctt ctagcctact ggggaccccg gtgagcggcg
     2161 tggccgaggc gccgtgacat cgccacggcg gcgtcgacgg tgacgcggcc gaggccgggc
     2221 agatcgatgg cgcggacgcg gaaggacggc ccggagacgg cgatggcggc cacgacctcg
     2281 ccgttagcct ggcggacagg agcggcgacg gcgttgaggc cctcctcgat ctcgtcctgg
     2341 atgcaggcgt atccccggat gcgggcctct tcgagctgct ggcggaggct cgcggggtcg
     2401 gtcacggtct gcggggtgta gacctgcagc ggccgcgcca gcagctggtc ccgcttggac
     2461 ggggagagcc aggccaggaa gaccttgccg ctggcgctgc agtgcagcgg ggtgcgctga
     2521 ccgatccagt tgccgctgac gatcaggcgg gggcttgcaa cctggtcgac gtagatcacc
     2581 tcgtcgttgg acagcacgcc gagggtgacg ctctcgcggg tacggtcggc cagttcctcc
     2641 agcaccgggc ggccggtgcg gaccaggtcg agcccggcca tggcggtgcc ggcgaggcgg
     2701 atgagcccga agccgagccg gtagcggtcg ctcaccgggt cacgctccac caagccgccg
     2761 ccggcgaggg tggccgccag gcgcgaggcg gtcgccttgt ggacgccgag gcggcggccg
     2821 aggtcggtca cgccaagctc accggcctcc gcgatcaact caagggcctg caatgcgcga
     2881 gtgaccgact gtacgccgcc acgtacttcg tcgctcatag cgcaactcta gcctgcgatc
     2941 ggcaatcctg tcccaggtag gcttgctggt atggcgatgc ggtactggcg ggcggcagag
     3001 ccgaagcgct cctacgacgt cgtcgtggtg ggcggcggcg ggcacggcct cgcgaccgcc
     3061 tactatctgg cgcgcaacca cgacctcacc gacgtctgcg tggtcgagaa gggctggctc
     3121 ggcggcggca acaccgcccg caacaccacc atcatccgct cgaactacct gtgggacgag
     3181 tccgccgcca tctacgagca ctccctcaag ctgtgggaga ggctggagga ggacctcggc
     3241 ctggacctgc gcttcagcca gcgcggcgtg ctgaacctgg cccacgacct cggcgagatg
     3301 cgctcgtcgc tgcggcgggt gaacgccaac gccctcaacg gcgtcgacgc cgagtggctc
     3361 accccggcgg aggtcgccgc gttctgcccg gttgtcaacg tctctcccga cgtccgctac
     3421 ccggtgctcg gcgcgaccct gcagcggcgc ggcgggatcg cccgccacga ccgggtcgcc
     3481 tggggcttcg ccagcgtcgc cgaccggctc ggcgtcgaca tcgtcgagca ctgcgaggtg
     3541 acgggattcg tccgcagccc tgccgggcgg gtgaccggcg tcgaaaccac ccgtgggacg
     3601 atcaccgcca acaaggtcgc tgtggtcgcc gccgggcaca cctcggtgct ggcggcgatg
     3661 gtggggctgc ggctgccgct gcagtcgcac ccgctgcagg cgctggtctc ggtgctgctg
     3721 acgccggtgc tggactgcgt ggtgatgtcc aacgcggtcc acgtctatgt ctcgcaggcc
     3781 gacaagggcg agctggtcat gggcgcgggc atcgacggcc acaacagcta cacccagcgg
     3841 ggcgccttcc acctgatcga ggagcaactc gccgccgcgc tggagctgtt ccccgtgttc
     3901 gctcacgcca agctgctgcg gacctggggc gggatcgtgg acgtctgccc ggacgcctcc
     3961 ccgatcatcg gcccgacccc gatcgagggc ctgtggctga attgcggctg gggcaccggc
     4021 ggcttcaagg cgaccccggg ctccggatgg gtgttcgccg agacgatcgc caagggcgag
     4081 ccggcaccgc tggcgctgcc gttcgccctc gaccggttca ccactggagc gttgatcaac
     4141 gagcacggcg cggcggcggt ggcgcattga tggcggcgcg ctgatgctgc tgctcccctg
     4201 cccgtggtgt gggctccgtg aggaggtcga gttccgctac ggcgggcagg ccgggatcgc
     4261 ctacccggcc gatccggacg ccctctccga cgaggaatgg gcggactacc tcttcatgcg
     4321 cgacaacccg aaagggccgt tcgcggagcg ctggtaccac gtgcacggct gccgccgctg
     4381 gttcaacctg gtccgcgaca ccgccaccaa cgagctgctc gcggagggac gctgacgtgc
     4441 ccggacccgg acccgggcgc cggctcgcct ccggcggcgc gatcgaccgg accaggacgc
     4501 tgggcttcac cttcgacggc cgggagtatg cggggctgga gggcgacacg ctcgcgtcgg
     4561 ccctgctggc aaatggcctc gccggcgggt tccaatcgcc gctgctcggc cgcccccgag
     4621 gggtgatgag cgccgggccc gaggagccgt gcgcgttcgt ggaggtgtcg gcaccgtggt
     4681 tcgacctgct cgcgccggcc acgatgctgc ggctggtcga cggccttgcc gccatcccgc
     4741 gcaccggcgt cgggcggctg ccgccaccgg atgcctcggc ggtggcggcg cgaccgcgcc
     4801 atgcccacgt cgagacgctg gtcgtcggcg gcggggtcgc ggggctgcgg gccgcgcggg
     4861 aggccgcgga gcgcggcgac cgggtcctgc tgttggacga gcggtcctgg ctcggcggga
     4921 ccgcccgctc cttcgagcag gtggaggggc ggccggcctg ggagtgggtc gaggagaccg
     4981 ccgccgcgct gcggagcgcg ccggacgtga cgctgctcac cgaggccacc gcgctcggcc
     5041 tctacgacgc tggctacgct gtcatccacg agcgctcccg tccggtcgaa catctctggc
     5101 atgtccgcgc gaagcgggtg gtgcttgcga ccggcgccca cgagcggccg gtcgcgttcg
     5161 ccggcaacga ccggccgggg gtgatgctgt ccggggcggt gctcacctat ctcgaccggt
     5221 tcggggtgct cccggcggag cgggcggtgg tttgggtcgc caacgacgct ggctacgcga
     5281 ccgcgcgggc gctccgggcg gcgggtgccg aggtggtcgc ggtggtcgac gcccggcgcg
     5341 aaggcgaggc cgccgcccag gaccaggccc gcgccgacgg cctggaggtg ctggccggga
     5401 gcgtgatcgc cgccaccgac gccgccaccg gctccgggca cgtcagcggg gcgttcgcgg
     5461 tcggacccga cgggcagcgg atccgcctgg aggccgagct gctggccgtc tcgggtggct
     5521 ggaacccggt ggtgcagctc caccgggcga tcggcggcgg ccttcgctac gaccccgccc
     5581 gcgcctgctt cgtacccgac ggcggaccca cctggctgtc ggcgaccggc gccgccgctg
     5641 gggaggttcc cgccgccgag ccggtctggc acatcgccgc tggggacggg gactccagcg
     5701 ccctctccac ctctctctcg gaacacttcg tcgacctgca gcgcgaccag acggtctccg
     5761 acgtcgccgg ggccctcgac gcgggcctgc gcagcgtcga gcacatcaag cgggtgaccc
     5821 acatcggcac cgccgccgac cagggccgca cctcggggac gctcgccgcc gagatcgtca
     5881 accagctcct cggcgccgac cccggcgcgc agggcccgac gggagcccgg ccgccgtggg
     5941 cgccgctccc ctaccacgtg ctggccgggc cgttccgggg ccggctgttc gacccggtcc
     6001 gcgagacccc gatccatccc tggcacgtgg cccacggcgc ggtgttcgag aacgtcgggc
     6061 agtggaagcg cccctggtat ttccagcgcg agggcgagtc gatggaggag gcggtcctgc
     6121 gcgagtgcgc ggcggcccgc gacggcgtcg ccctgatgga cgcctcgacc ctcggaaaga
     6181 tcgaggtggt cggcccggac gccgcgacct tcctcgaccg gatgtacacc aacgcgatct
     6241 ccaccctgcc ggtcgggcgg ctccgctacg gggtcatgct cggcctggac gggatggtct
     6301 tcgacgatgg cgtggcgatg cggctggccg ccgaccgcta cctggtcacg accaccaccg
     6361 gcaacgcggc ggcggtgctc gaccgcttcg aggagtggct ccagaccgag tggcccgagc
     6421 tgcgttgctg ggcgaccagc gtgaccgagc agtgggccac gatcgccgtc gtcggccccc
     6481 tggcccgtga ggtcctgtgg gccgccggca ccgacgtgga cgtcagcacc gcggcgttcc
     6541 cgttcatggc ctggcgcgac ggcaccgtcg ccggcgtgcc ggcccgggtc acccgggtgt
     6601 cgttctcggg cagcctcgcc ttcgaggtca atatcgccag ctggcacggc ctccaggtct
     6661 gggaggccct gtacgccgcc gggcagccgt tcggcttgac cgtctacggc accgagacca
     6721 tgcacgtcct ccgcgccgag aaggccttcc cgatcgtcgg ccaggacacc gacggcaccg
     6781 tcaccgccga cgacctcggc atgtcctgga tcgtcaacct ccgcaagggc gacttcgtcg
     6841 gccgccgctc gctccgccgc gccgacctga cccggccgaa ccgcaaacag ctcgtcggcc
     6901 tgctccccca ggacaccgct gcattgattc cggagggcgc ccagctgatc gaccccggct
     6961 ccgtgggcgc ctcaccaccc gtgccgatgc ggggccatgt gacctcgtcc taccgcagcg
     7021 ccgcgctcgg ccggaccttc gcgctcgcgc tgctcgaagc cggccggcaa cgccacggcg
     7081 acaccgtctt cgcgttcgac gcccgcctca ccgaaccgat cgccgcaacc gtcgccgacc
     7141 cagtgttcta cgacccggag ggagcacgcc gcgatggctg acgtcagcat ggtggaggtc
     7201 ccaaccctct cccaggtgag cctgcgggta gacctgacac cagcgcaggg ccggcctgca
     7261 ccaccgctcg agctcccgct ccagccgaac aattggcggc ggattgggat cctggaggcc
     7321 ctatggctcg gccccgacga atggctggtg accgccggtc aaggcaacac cggaaagatg
     7381 gtgatcctgc tggaggccgc cttcgacgga aagccccact ccgtggtcga cgtcagcgcc
     7441 aaccgcatcg ccatcgacct gaccggcccc gaccggctcg acctgctggc gaccggctgc
     7501 cccctcgacc tgcacccccg tgtctggaat gagggcgcct gcgcccagac cctgctggcc
     7561 cgcgtgcccg tcctgctcca ggaacgcgcc gccgggaccc gcctgttcgt gcgcccgtcg
     7621 ttcgcagcct ggctgaccga ctggctggac gccgctgccg cggtctcgta gccgtcagac
     7681 gtttgagtcg gggaacgagg ctttgcggcg ttcgatccac tcgcgcagct cctggtcgac
     7741 ggcgtcgtcg attggagggg gctggtagtc ggcgagggtc tttttccaga gggcgttggc
     7801 gcgttgggcg gcgtcgcggg agccctccag gtcccattgc tcgaaggagt tggcgtcggc
     7861 gacctgggag cgccaaaagg cggtctcgaa gttggccagg gtatgggcgg agccgaggaa
     7921 atgctggccg ggaccgttgg cgatgatcgc gtccagggcc tggccgttcg gggaaaggtc
     7981 cacgccccgg gcgaaggcgt gccaggcgcc ggcctggtcg ttgtcgagca cgaacttctc
     8041 gtagcccatg cagaggccgc cctccagcca accggccacg tgcagcacga agttcacgcc
     8101 gccgagcatg gtcggctgca tggtgttggc cgactcgaag gcagcctgcg cgtcggggac
     8161 cttggcggcg gtgagcgcgc cgccgctgcg gaacgggacg ccgagccggc gcgcgcaggc
     8221 ggccagggcg tagagcacca gggcgggctc gggggtaccg aaggtgggcg cgccggactg
     8281 catcgacatc gaggaggcga acgagccgaa caccacaggg gcgcccggcc gcaccagctg
     8341 cacgtaggcc atgccggcca gcgcctcggc gagggtctgg gcggcggtcc ccgccacggt
     8401 cgccgggctc attgctcccg ccaggatgaa cggggtcatc agggtggcct gattggcctc
     8461 ggcgtagacg cgggcggcgc cgagcatggt ggcgtcccag accagcgggg aattcgcgtt
     8521 gatcaggctg aggatcacgc agtcgcgctc cagccgctcg gcgccgaaca cgatccgggc
     8581 catcgccacg gtgtcgctgg cgcggtcggg gtgggtgacc gagcccatga acggcttgtc
     8641 ggagaggcgc aggtgtgcct cgaccatgtc gaagtggcgc ttgttgaccg gcaggtcgac
     8701 cggctcgcag acggtcccgc cgctgtggtg cagccagggc gaggcgtagg tgagcttcac
     8761 gaagttgcgg aagtcctcaa tggtcccgta gcggcggccc tggtccaggt cgcggacgaa
     8821 cggcggccca taggcggggg cgaacaccgt ggcgtccccg ccgatgcgca cgtcgttggc
     8881 agggttgcgg gcgtgctggg tgaactcgcg gggcgcggtc gcctgcacca gcgcgcgggc
     8941 catgccgcgg ggaaagcgga ccagctcgcc gtccacctcg gcgccggcgt ccttgagggt
     9001 ggcgagcgcg tcggcgtcgc ggaactcgac gccgacctgc tccaggatca cgtcggcgtt
     9061 gtgctcgatg agggcgaggc cctcctcgga aaggacctcg aacggcgcca tcgtgcgggt
     9121 gatgaacggg accctctcga cgaccgcgtg cagccgtgcg gcgcggcgcc ccccgcgccc
     9181 gccgctgcgc cgccccctcg gctcggcctg gttgctcacc gggtcctcct tctggtcctg
     9241 gtcctggggt gcatccgccc gttcgcgtcc agatcgcgct cggggagcgt gaccaccttg
     9301 gcgaggcggg cgtaggggtc acggcggtgc ggaatcgcca tcgcgtcgac gaagtgttcc
     9361 tggaagcgcg gctcgacggc ggtctcgacc ttctcgacgc gccgcacggc ttcctcgacc
     9421 tcgcggcggg agcggccaga gagcagcgcg atgcgggcgc cggtcccggc ggcgttgccg
     9481 gcgctgctga ccgccgcggg gtcgcagtcg gggaccatgc cgagcacgag ggcgtggacc
     9541 gggtcgatat gggcgccaaa cgcgcccgcc aggcggaccc ggtcgacctg gtccacaccg
     9601 aggcggtcca tcagcagccg gcagccggcg tgcagggcgg ccttggcgag ctggatctgg
     9661 cggatgtcgt tctgggtgac caggatccgc tggagggcgc ggtcgtgcag cacgtaggca
     9721 tacgtgcgcc cgtcggggac gacccgggag gagcgcgcgg cggcggcgcc gtcgatcacg
     9781 ccggcggcgg tgacgacccc ggcgaggtac agctcggcga ccgcctcgat gatccctgag
     9841 ccgcagatgc cgctgacagc agcaggatcg aagcccggct ggtcggacca ggccgggttg
     9901 ccgatcacgc ggaggcgcgg ctccagggtg tcgcggtcga tgcggacgcg ctcgatcgcg
     9961 ccgggtgcgg cgcgctgccc ggcggagatc tgcgcgccct cgaacgccgg gccagtgggg
    10021 ctggaggcgg ccagcagccg gtcgcggttg ccgagcacga tctcggcgtt ggtgccgacg
    10081 tccaccagca gcgacaggtc gtcgcgctgg tggggcgcct cggccagcag caccccggcg
    10141 gcgtcggcgc cgacgtgccc ggcgatacac ggcaggacgt aggcgcgggc gcccggatgc
    10201 acgcccagct ccagctcggt ggcggcatgg aggcgcagcg cctggccggt ggccagcgcg
    10261 aacggcgcac cgcccagctc cgtcgggtcg atgccgagcg ccaggtggtg catcaccggg
    10321 ttgccgacca gggtcacctc cagcacgtcg ccgggctcgc ggcccgcctc ggcgaccagg
    10381 gcgacgacca ggtcgttgag gcagccgcgg accgcgcggg tcagctcagc ggtcgagccc
    10441 gggttgagca tcacgtagct gacgcggctc atcaggtcct cgccgaagcg gatctgtggg
    10501 ttcatcgcgc ccgcgctggc gagcacctcg ccggtggcga ggtcgcagag gtgcccggcg
    10561 acggtggtcg agccgacgtc gaaggcgatc ccgagggcga cgtcgcggaa gcccggccag
    10621 accgccgtga tcgcgctgcc gtcgtggacg gcgacggtga cggcccactc ccccttccgc
    10681 agcgcctgct ggaggccctg gacgacgtgt atgtcggcgg tcagaccagt gaggccccac
    10741 tcgcgctcca gcgcttcgag gaggcgggcg tggtcgctgg tctgggtgcc gagggcgggc
    10801 tgctcgacct ccacgtagca gaggcgcacc accgggtcca gctcgatcgc acgcgcctcg
    10861 gcgggcttgc gcaccacctg ccgatggagc tggctgtcgg gcggcacgtc gatcaccagg
    10921 tcgccgacca tgcgcgcgtg gcagccaagc cgccgctccg ggtcgagccc ccgccgggcc
    10981 cggtaggttg cctccggcgc cgcccacggc gtgagatggt cagcccgcga ttcgatctgg
    11041 agcttcgcgt gcgccccctc gctcacccgg atctgacacc gcccgcagat cccccgcccg
    11101 ccgcacaccg agtcgaggtc aaccccaagc gctcgcgcgg cctccagcac gctggtcatc
    11161 accgggaacg accctcgccg cccggagggt gtgaagatga cccgcacttc attcacaaat
    11221 cttgtgccgc gcggcggcgt ccctcccggc cacctcgacg ccgcccagcg ccgccgtcgg
    11281 aggtaccggg ctgagcgccg tcgcggtagc ggctgatcca ggtcatgcag tcgcggtcgt
    11341 tgccgaggag cacgtcggcg gcggcgaccg cgcggcggac gtcctcgtgg agcggattgg
    11401 tgatcgccga ggtcatgccg gcggcggcgg ccatggtcag gaacgccgcc gacagcgcgt
    11461 ggcggttcgg cagcccaaag gcgatgttgg aggcaccgca ggtggtgttg acgtgcagct
    11521 cctggcgcag gcggcggacc aggtggaaca cctgccgccc ggcgctcgcc accgcgccga
    11581 tcggcatcac cagcgggtcc accacgatgt cggcaggggt gatgccgtag tcggcggcgc
    11641 gctcgacaat cttgcgggcg acctcgaagc ggacgtcggg gttctcggag atgccgcgct
    11701 cgtcgttgct gatcgccacc accgcggcgc cgtgcctggc gaccagcgac agcacgcgct
    11761 gcaactgctc ctcctcgccg gtgactgagt tgaccagggc tttgcctttg tagacgggca
    11821 gcgccgcctc cagcgcctcg acgattgagg agtcgataca cagcggcaca tccgtcaccg
    11881 actggaccag ctcgaccacg cgggtgagga tcgcgggctc gtcggccagg ggaacaccgg
    11941 cgttcacgtc cagcatgtgg gcaccggcct cgacctgcgc gagggcgtcc gcgatcacgg
    12001 tggagaagtc gccgatcgcc atctcggcgg cgaggcgctt gcgtcccgtc gggttgatcc
    12061 gctcgccgat gatcacaaac ggccggtcga acccgatcac gacctccttg gaggccgacg
    12121 acaccacggt atgggtcatg cgcacgccac ctccacagct cgctgcctcg cagcctggag
    12181 tgccgggacc aggtcgccgt agccgctgtg gcggacctcc agggtgaggc cgaggtaggc
    12241 cgccgcctcc ttcgccttgg cgagcagcgc ttcgtcgtcg cgctgcgcga ggtagaccag
    12301 gcgcacgtag ttgccgaaat aggccggcag cagctccgga tgccggtcca ggccgaggcc
    12361 ccgcaccacc agccggtcga agtggcggca caggaagtcg gtcaggtaga aggtccgcgg
    12421 ctccgcgtca tgcagtgcga gccaggagtc cacccccgcg aagaaggcgt agcagtggtc
    12481 gccgggaagg cgcccgacgc cgtgctcgtc caggacgcgg tcgagcgccc cgccggtgcc
    12541 gcagtcgccg taggcgacca ggatc
//
LOCUS       DASWDC010000152         7494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_868619, whole genome shotgun sequence.
ACCESSION   DASWDC010000152 DASWDC010000000
VERSION     DASWDC010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868619"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VF995_04850"
     CDS             <1..210
                     /locus_tag="VF995_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342925.1"
                     /translation="NLTSGEPANLTSGEPANLTSGEPANLTSGEPANLTSGEPANFTI
                     DPVGGHVLVVPTIDGCPSTPTTGLK"
     gene            238..774
                     /locus_tag="VF995_04855"
     CDS             238..774
                     /locus_tag="VF995_04855"
                     /inference="COORDINATES: protein motif:HMM:NF015928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase inhibitor family I36 protein"
                     /protein_id="HEX9342926.1"
                     /translation="MTVYARLRTPPRLVALLVVALVAALAWPAPDSAQAGSGLRQARA
                     RVAAGDLLYPDGIRVSLGVGKGLQPGQHQNPAAASAAAVIPCTAGYTCLYEHVNYGGR
                     RLEWQDPGLLVNLADYDFNDQMSSWRNLSGDAAIWYVDADGLGLAHCMPAGASDPWVG
                     SSDNDQASSLRLLTQQTC"
     gene            854..1111
                     /locus_tag="VF995_04860"
     CDS             854..1111
                     /locus_tag="VF995_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX9342927.1"
                     /translation="MIIIGWLLIGLVAGALARLLMPGRDPLGCFGTALLGVIGSFVGG
                     FLYELIAYHQATLHRSGLIGSVVGAMLLLLLRRLFVSESRR"
     gene            complement(1137..2726)
                     /locus_tag="VF995_04865"
     CDS             complement(1137..2726)
                     /locus_tag="VF995_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014627526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX9342928.1"
                     /translation="MVRRLAAWLLAFAVAAGAVVAAREAVPRLRTRIRHAVGIGLPAA
                     KTQPAAREILVGDPGWIAAENRRPGTTAWRITKRGDPAALAGYASRVSAARGEVVALF
                     VSTTAPRFQVEAYRMGWYGGKHGRLLWRSPPVPGRVQAPARTTPLTDLVEAPWRPSLR
                     FRVDAGWPPGDYLLKLVASTGWQRYVPLTVTDQTSVDPLVVMNAVTTWQAYNDWGGRN
                     LYWGPFREAELRSRVASFDRPYLEGDGASDFLNGELPLVSLVEEAGLDAAYWTDVDLH
                     EHPELLLRHRALLSLGHDEYWSTAMRLGATDARDHGVNLAFLGANAVFRHIRLEPSPL
                     GPDRREVNYKPQSAFADPAWRTNRKEVTTDWREPPLNQPESALLGELYECNPAHADEV
                     ISAPASSWLLAGTGLGPGDRLPNLIGPEYDRVMLDEPTPHGVELIAHSPLRCRFHASF
                     SDVTWYSTSSGAGVLDTGTSSWVCQLDHACAAGRNSPVTAAAVRRITLNLLRAFAAGP
                     AGLAHPSVPNLKHLDLRPSGL"
     gene            complement(2804..3763)
                     /locus_tag="VF995_04870"
     CDS             complement(2804..3763)
                     /locus_tag="VF995_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX9342929.1"
                     /translation="MKKLLLLLFLTPIILFALIGLLLMGVATGIIGGARPSATAVMDI
                     PANYLALYEAAADTCPGLPWQVLAGVGKEESNHGRSTLPGVSSGENFAGAGGPMQMGI
                     DPALPAGAAFWKYAKVDVDGGGISPYSPADEIFAAANYLCANGAAGGHNVSNAIFQYN
                     HAQWYVDKVLGFASSYAAQVVLPAGASEQQIVNQVVTFGWDQLGHPYQWGAVGQHGFY
                     DCSGLTLAAYRTAGIQLPRTAAEQWTAGARVYDLGALQPGDLVFYANNLSDPSTIHHV
                     GIYIGAGNMIDAPHTGAVVRIEPFLRGDFIGAVRPTASPIAGS"
     gene            4100..5389
                     /locus_tag="VF995_04875"
     CDS             4100..5389
                     /locus_tag="VF995_04875"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEX9342930.1"
                     /translation="MKQLRSPLIAAADEAYVPLGSPGVGPPDVPKSPPPPPQSPSQSR
                     RQSPPEPSRSQPGGQDGRRHGQADRQPPVPTSPPTSPPTSPTRFRDRPLPEAPPSGAP
                     DGPQLTRMQRQMGVTSPAPPGSTASAADFTADHFLRDRGAPPRDGWRRALYWLTGGRV
                     NPGPSPLEVAQRELIARAKTPVPGCRSIAVISRKGGVGKTTTTLMLGHTFASLRGDRV
                     VALDGNPDAGSLGYRVRRETAATITNLLADEQMITRYADIRAYTSQAPTRLEVVASDD
                     DPRITKALGEVDYRRAIGLLERHYNLICLDTGTGVLESATRGILQLADQIVVVMSPSL
                     DSARAASSTLDWLNENRHAELVRSAVAVVNGIQDDLLVEVGRIQEHFAGRCRAVVQVP
                     WDPHLAAGAETEVGRLRPATRHAYLEVAATVADGFLT"
     gene            5490..5795
                     /locus_tag="VF995_04880"
     CDS             5490..5795
                     /locus_tag="VF995_04880"
                     /inference="COORDINATES: protein motif:HMM:NF039351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6112 family protein"
                     /protein_id="HEX9342931.1"
                     /translation="MHAHAQLLAAKLNIHMTPNFSGIPAAGPIQTIIDDLGAFALFLS
                     LAGIIIGGAMWGVGSLSSNYHQAAVGKRATLYSVAGAIVIAAAAALVNWAFQLGSTA"
     gene            5827..6702
                     /locus_tag="VF995_04885"
     CDS             5827..6702
                     /locus_tag="VF995_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342932.1"
                     /translation="MAIDERDRARVVYRLPEPRQAGPGSGLGLFAVLGVALLLFGGGL
                     LIGRLTTSSHPSTRARTAQTTTVATAGGAATATTAPALAVPDAGAATSRVGPAGSRNG
                     VPVGYQHSSQGAVAAATNYTAVLSSDLILDQTRREAAIGTLAAPEARAALERVFARTV
                     PAIIQALGDSGTGAAAAAGAGSGTTGNAGAGGKVVLRFIPVGWRLERYDGATAEVSIW
                     ATGIGGSLNGVPVQEGWGITTVELRWTDNDWKETSATTRDGPVPVADDSAPTAASVIV
                     PEAQQFKEYHYAPGS"
     gene            6686..>7494
                     /locus_tag="VF995_04890"
     CDS             6686..>7494
                     /locus_tag="VF995_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342933.1"
                     /translation="MRQGRELPRAGARRLLLVAALLATSYLVAVPAFAAPPTTAPTAA
                     PAQTQGQQQAAATPPANDCAKLSGPAKKLCDLAKHPGGAVSGAVGGAAAGVVSTAGDS
                     ALRSFTAAVASAGKWFLDKVGGLLRGTTSPNVVSAAWFVNQYRVMLALAVLIALPVLL
                     VSVAQSIVRVDMMQAVRSAVVYLPIAAIFSAVAPAIVQLLIDSSDWISTAITHSASAS
                     AQQFMTDTGGWLGAIGAGTANPAVPVFGVLLGAMVVVLGAFSIWLELLLRSA"
BASE COUNT         1100 a         2802 c         2542 g         1050 t
ORIGIN      
        1 aatctcacca gcggcgaacc cgccaatctc accagcggcg aacccgccaa tctcaccagc
       61 ggcgaacccg ccaatctcac cagcggcgaa cccgccaatc tcaccagcgg cgaacccgcc
      121 aacttcacca tcgacccggt cggcggccat gtcctggtcg tcccgacgat cgatggctgc
      181 ccctccacac ccaccacggg actgaagtga cgccaggacg ttgccgatat tgcctaggtg
      241 accgtctacg cgcgtctgag aactccgcca cggctcgtcg ccctcctggt cgtcgcgctt
      301 gtggccgcgc tggcctggcc ggccccggac tccgcccagg caggctccgg gctccgccag
      361 gcccgcgcca gggtcgccgc gggcgacctg ctctatcccg atggcatcag agtctcgctc
      421 ggcgtcggca agggcctgca acccggccag caccagaacc ccgccgccgc cagtgccgcc
      481 gcggtgattc cctgcacggc cggctacacc tgcctctacg agcacgtgaa ctacggcggc
      541 cggcgcctcg aatggcagga ccccggcctg ctggtcaacc tcgccgatta cgacttcaac
      601 gaccagatga gctcctggcg caacctcagc ggcgacgccg ccatctggta cgtcgacgcc
      661 gacggcctcg gcctggccca ttgcatgccc gccggcgcca gcgacccctg ggtcggctcc
      721 agcgacaacg accaggcctc ctccctgcgc ctgctgaccc agcagacctg ctgacccggt
      781 cgtccgcgcg aagctgacgt acgctggtcc cagtggcccc ctggccacgg cagggcagga
      841 gctgggagag cacatgatca tcatcgggtg gctgctgatc ggattggtcg ccggggcgct
      901 ggcgcgcctg ctgatgccgg gccgggaccc gctcggctgc ttcgggaccg cgctgctcgg
      961 cgtcatcggg tcgttcgtcg gcgggttcct ctatgagctg atcgcctacc accaggccac
     1021 gctgcaccgc tccgggctga tcggatcggt ggtcggcgcc atgctgctgc tgctgctgcg
     1081 ccgcctgttc gtcagcgaga gccgccgttg aggatcagcg gagccgcccg ggggcctcag
     1141 aggccgctcg ggcgcaggtc gaggtgcttc agattcggca ccgacgggtg cgccagcccc
     1201 gccgggccgg cggcgaaggc gcgcagcagg ttgagggtga tccgccgcac cgccgcggcg
     1261 gtgaccggac tgttccggcc agccgcgcag gcgtgatcca gctggcagac ccagctggag
     1321 gtgccggtgt cgagcacgcc cgcgccgctg ctggtggagt accaggtgac gtcggagaag
     1381 ctggcgtgga agcggcagcg tagcggcgag tgggcgatca gctcgacgcc gtgcggggtc
     1441 ggctcgtcga gcatcacccg gtcgtactcc ggcccgatca ggttggggag ccggtcgccc
     1501 gggccgagtc cggtgcccgc cagcagccag gaggaggccg gcgcgctgat cacctcgtcg
     1561 gcgtgggcgg ggttgcattc gtacagctcg ccgagcaggg cgctctccgg ctggttgagg
     1621 ggcggctcgc gccagtcggt ggtgacctcc ttgcggttgg tgcgccaggc cgggtcggcg
     1681 aaggccgact gcggcttgta gttcacctcg cggcggtcgg ggccgagcgg cgagggctcc
     1741 aggcggatgt gacgaaagac cgcgttggcg ccgaggaagg cgaggttgac gccatggtcg
     1801 cgggcgtcgg tggcacccag gcgcatcgcc gtcgaccagt actcgtcatg gccgagcgac
     1861 agcagcgccc ggtggcgcag cagcagttcg ggatgctcgt gcaggtccac gtcggtccag
     1921 taggcggcat ccaggccggc ctcctcgacc aggctgacca gcggcagctc gccgttgagg
     1981 aagtcgctgg cgccgtcgcc ctccaggtag gggcggtcga aggaggcgac ccgggagcgc
     2041 agctcggcct cgcggaacgg cccccagtag aggttgcgtc cgccccagtc gttgtaggcc
     2101 tgccaggtgg tcaccgcgtt catcaccacc agcgggtcga cgctggtctg atcggtgacg
     2161 gtcagcggga catagcgctg ccagccggtg gaggcgacca gcttgagcag gtagtcgccc
     2221 ggcggccagc cagcgtccac ccggaaccgc agtgaaggac gccacggcgc ctccaccagg
     2281 tcggtgagcg gcgtggtgcg cgccggcgcc tgcacccgcc ccggcaccgg cggggagcgc
     2341 cacagcagcc gcccgtgctt cccgccgtac cagcccatgc ggtaggcctc cacctggaag
     2401 cggggggcgg tggtggagac gaacagcgcc accacctcgc cgcgcgccgc gctcacccgg
     2461 ctggcgtacc ccgcaagcgc ggccgggtcg ccccgcttgg tgatgcgcca ggcggtggtg
     2521 ccggggcggc ggttctcagc ggcgatccag cccggatcgc cgaccaggat ctcgcgggcc
     2581 gccggttggg tcttcgccgc tggcaggccg atgccgacgg cgtggcggat cctggtgcgc
     2641 agccgcggca cggcctcgcg cgccgcgacc acggcccctg ccgccaccgc gaacgccagc
     2701 agccaagccg ccagacggcg caccatcgcg ccgcgctcgt cccccccgag tcgtcgccaa
     2761 cttgttatct cgatcctccc ggtgcgggag tttcagggag atctcacgag cccgcgatcg
     2821 ggcttgccgt cgggcggacg gcgccgatga aatcgccgcg caggaacggc tcgatccgta
     2881 ccaccgcgcc ggtgtgcggc gcgtcgatca tgtttcccgc gccgatgtag attccgacgt
     2941 ggtggatggt cgacgggtcc gacaggttat tggcatagaa caccagatcg cccggctgca
     3001 gcgcgccgag gtcgtagacc ctggcgccgg ccgtccactg ctcggcggcg gtgcggggca
     3061 gctggatccc ggcggtccgg taggcggcca gcgtcagccc tgagcagtca tagaagccgt
     3121 gctggccgac agcgccccac tggtagggat ggccgagctg atcccagccg aaggtcacca
     3181 cctggttgac gatctgctgc tcggacgcgc ccgccggcag caccacctgg gccgcatagg
     3241 acgacgcgaa tcccagcact ttgtcgacat accactgcgc gtggttgtat tggaagatcg
     3301 cattgctgac gttatgaccg cccgccgcgc cattcgcgca caggtaattg gcagcggcaa
     3361 agatctcgtc cgcggggctg tagggactga tcccaccgcc gtcgacatcc accttcgcgt
     3421 acttccagaa cgccgcgccg gcgggaagtg ccggatcgat tcccatctgc atggggccgc
     3481 cggcgcccgc gaaattctca ccggatgaca cccccgggag cgttgaccgc ccgtggttgc
     3541 tctcctcctt gccgactccc gcgagcacct gccaaggcag gcccggacag gtgtcggcgg
     3601 ccgcctcata cagcgcgagg tagttggcgg gaatatccat gaccgcggtg gcgctgggcc
     3661 tggccccacc gatgatcccg gtggccacgc ccatcaggag caggccaatg agggcaaaga
     3721 ggatgatggg ggtgagaaac aacaggagca gcagcttctt catgccatct cggtcctggg
     3781 ggcggtcacg acggtgggat cgaggtgtac cacggcgccg ccgtggggga taggcgaaag
     3841 ttccccacct ccgccacggg tgatggtatt ttagttagtc gagagggttg gcgggatgcc
     3901 cggtcgcacc tgcgccggga agaagcggga acgcgcacac gcttttgcgt cattgccgag
     3961 cagtgtaacc tttgacacgc aacgcgggtg cgcagccttc cccacttccc cgagccgccg
     4021 cggagcaaac attgaataca acaagattca cccacatacg gacggtctgt ctgcctcaag
     4081 cggctacagg gggttcgccg tgaagcagct gagaagcccc ttgatcgccg ccgccgacga
     4141 ggcctacgtc ccactcggca gccctggcgt cggcccaccc gacgtcccga agtcaccacc
     4201 gccaccgccg caatccccat cgcaatcacg gcggcaatcg ccgcccgagc cgtcgcgatc
     4261 gcagccgggg gggcaggacg gccgccgcca cggccaagct gaccgccagc cccccgtccc
     4321 cacgtcgcca cccacgtcgc cacccacgtc gccgacccgc ttccgcgacc gcccgctccc
     4381 cgaggccccg ccgagcgggg cgcccgacgg gccacagctc acccggatgc agcgccagat
     4441 gggcgtcacc agcccggcgc cgccggggtc gaccgccagc gccgccgact tcaccgccga
     4501 ccacttcctg cgagaccgcg gcgcaccgcc gcgtgacggc tggcgccgcg ccctctactg
     4561 gctcaccggc gggcgcgtca atcccggtcc gagccccctg gaggtcgccc agcgcgagct
     4621 gatcgcccgc gccaagacgc cggtccccgg ctgccgctcg atcgccgtga tcagccgcaa
     4681 gggcggcgtc ggcaagacca ccaccacgct gatgctgggc cacaccttcg cgtccctgcg
     4741 cggcgaccgg gtggtggccc tggacggcaa ccccgacgcc ggctcgctcg gctaccgggt
     4801 ccggcgcgag accgcggcca ccatcaccaa cctgctcgcc gacgagcaga tgatcacccg
     4861 ctacgccgac atccgcgcct acacctcgca ggccccgacc cggctcgagg tggtcgcctc
     4921 cgacgacgac ccgcgcatca ccaaggcgct cggcgaggtc gactaccgcc gcgccatcgg
     4981 cctgctggag cgccactaca acctgatctg cctggacacc ggcaccggcg tgctggagtc
     5041 ggccacccgg gggatcctcc agctcgctga ccagatcgtg gtggtgatgt cgccgagtct
     5101 cgactcggcg cgggcggcca gctccaccct cgactggctg aacgagaacc gccacgccga
     5161 actggtgcgc agcgcggtgg ccgtggtgaa cggcatccag gacgacttgc ttgtcgaagt
     5221 cggccgaata caggaacatt tcgctgggcg ctgccgcgcg gtggtgcaag tgccgtggga
     5281 cccccacctc gcggccgggg cggaaaccga ggttggtcgc ctccgtccag ccacgcggca
     5341 tgcctacctc gaggtggcgg ccaccgtcgc cgacgggttc ttgacgtgac cgtgcacgcc
     5401 acgaccgcgg gccgccgccg acggactcgg cactgtagaa accgcagggc tacctggagc
     5461 tacctggagg aacgcaagga ggacgtgcga tgcacgcaca cgcacagctg ctcgccgcga
     5521 agctgaacat ccacatgacg cccaacttct ccggcatccc cgctgccggg ccgatccaga
     5581 cgatcatcga cgacctcggc gccttcgcgc tgttcctgtc gctggccggg atcatcatcg
     5641 gcggggcgat gtggggcgtc gggtcgctca gctccaacta ccaccaggcg gcggtcggca
     5701 agcgggcgac gctctactcg gtcgccggcg cgatcgtgat cgccgccgcc gcggccctgg
     5761 tgaactgggc cttccagctc ggctcgacgg cctgagcccg gaatcggaac ggggagcgtc
     5821 ttcacggtgg cgatcgatga gcgggacagg gcccgggtgg tgtaccgcct gcccgagcca
     5881 cgccaagctg ggcccggctc ggggctcggg ctgttcgccg tgctcggggt cgccctgctg
     5941 ctgttcggcg gtggcctgct gatcgggcgc ctgaccacgt cgtcccatcc ttctacccgt
     6001 gcccgaaccg cgcaaaccac caccgtcgcg accgcgggag gcgcggcgac cgcgaccacc
     6061 gcgcccgcac tcgcggtgcc cgacgccggt gccgccacca gcagggtcgg cccggccggc
     6121 agcaggaacg gcgtccccgt cggctaccag cactcctccc agggcgccgt cgccgccgcc
     6181 accaactaca ccgccgtgct ctccagcgac ctgatcctcg accagacacg ccgtgaggcc
     6241 gccatcggga cgctcgccgc ccccgaggcg cgcgccgcgc tggagcgcgt cttcgccagg
     6301 accgtccccg ccatcatcca ggcgctcggc gactccggca ccggagccgc agccgcagcc
     6361 ggagccggct ccggcacgac cggcaatgct ggcgctggcg gcaaggtggt cctgcgcttc
     6421 atcccggtcg gctggcggct ggagcgctac gacggcgcca ctgccgaggt gtcgatctgg
     6481 gccaccggca tcgggggctc cctcaacggc gtccccgtgc aggaaggctg gggcatcacc
     6541 accgtggagc tgcggtggac cgacaacgac tggaaggaga ccagcgcgac caccagggac
     6601 ggcccggtcc ccgtcgccga cgacagcgcc ccaaccgccg cttcggtcat cgtgcccgaa
     6661 gcgcagcaat tcaaggagta ccactatgcg ccagggtcgt gagctccccc gtgcaggcgc
     6721 gcgccggctg ctgctggtcg ccgccctgct cgccacgtcc tacctggtcg ccgtcccggc
     6781 cttcgccgcc ccgccgacca ccgcacccac cgccgccccc gcccagaccc agggccagca
     6841 gcaggccgcc gccacgccgc cggccaacga ctgcgccaag ctgtccgggc cggcgaagaa
     6901 actctgcgac ctcgccaagc atcccggcgg cgcggtctca ggggcggtcg gcggcgccgc
     6961 cgccggggtg gtctccaccg ccggtgacag cgccctgcgc agcttcaccg cggcggtcgc
     7021 ctcggccggc aagtggttcc tcgacaaggt cggcggcctg ctgcgcggca ccaccagccc
     7081 gaacgtggtc agcgccgcct ggttcgtcaa ccagtaccgg gtgatgctcg ccctggcggt
     7141 gctgatcgcc ctgccggtcc tgctggtctc ggtggcgcag tcgatcgtcc gcgtcgacat
     7201 gatgcaggcg gtccgctccg ccgtcgtcta cctgccgatc gcggcgatct tctccgccgt
     7261 cgcccccgcc atcgtccagc tcctgatcga ctcaagcgac tggatctcga ccgcgatcac
     7321 ccacagcgcc tccgcctcgg cgcagcagtt catgaccgac accgggggct ggctcggcgc
     7381 catcggcgcc ggcaccgcca atccggccgt gccggtgttc ggcgtgctgc tcggcgcgat
     7441 ggtggtggtg ctcggcgcct tctcgatctg gctggagctg ctgctgcgct cggc
//
LOCUS       DASWDC010000153         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1621648, whole genome shotgun sequence.
ACCESSION   DASWDC010000153 DASWDC010000000
VERSION     DASWDC010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621648"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..454)
                     /locus_tag="VF995_04895"
     CDS             complement(32..454)
                     /locus_tag="VF995_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342934.1"
                     /translation="MFKTLARERLGWVVAGIMALALVGGGVAYAVTRNHGGASTDSAS
                     KAANGGAVRGRVMALSAESLTLRVRGGTTMNVLLGAKTRYGNNAHPASRADLRVGETV
                     RVVGDASGATVTAKRVVIVSGGGNGDSGSATTTSTRST"
     gene            complement(479..1729)
                     /locus_tag="VF995_04900"
     CDS             complement(479..1729)
                     /locus_tag="VF995_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9342935.1"
                     /translation="MTRVATEVREAVQRVFREESGKAVATLIRQLGDFDRAEEAVAEA
                     FLVACERWPVDGVPPRPGAWITTTARNKAIDRLRRERGFAERQPALALLEAERQEAEE
                     ETHTIGDDRLRLIFTCCHPALSLEARVALTLRALGGLTTPEIAHAFLVPEATMAQRLV
                     RAKRKIRDAGIPYRVPPDHLLPERLGAVLAVLYLVFNEGYSASAGDDLVRRELSGEAI
                     RLCRLLAALMPDEPEALGLLALLLLQDSRRQTRTGPDGDLVLLEDQDRTRWDHEEIGE
                     GVRLLDRAMRMGPPGPYRLQACVAALHATAPAADATDWRRIAALYERLGALLPSPVVA
                     LNHAVAVAMADGPMCGLALVEELAAGGELEDYHLLHATRADLLRRLGRLEAAGAAYRR
                     ALDLATNPVEQRYLRRRIAEVAIS"
     gene            complement(1765..2124)
                     /locus_tag="VF995_04905"
     CDS             complement(1765..2124)
                     /locus_tag="VF995_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9342936.1"
                     /translation="MRYALLIYGSEAAMATATEAETQAEFDAYNSFTKEIVDRGLMQG
                     GEALQPTSTATTVQVRNGETLTTDGPFAETKEQLGGFYLVDCRDLDEAIATAAKIPGA
                     KSGSIEVRPIMEIPPVS"
BASE COUNT          277 a          825 c          773 g          321 t
ORIGIN      
        1 gccagcgcgg ctgagctcgc gcgcctcctg gctaggtcga cctggtcgag gtggtggtcg
       61 cgctgccgct gtcgccgttc ccgccgcccg agacgatcac cacccgcttg gcggtgacgg
      121 tcgcgcccga ggcgtcgccg accacgcgga cggtctcacc gacccgcagg tccgcgcgcg
      181 aggccgggtg ggcgttgttg ccgtagcggg tcttggcgcc gagcagcacg ttcatcgtgg
      241 tgccgccgcg cacccgcagg gtgaggctct cggccgacag cgccatcacc cgcccgcgga
      301 cggcccctcc gttcgccgcc ttggaagcgg agtccgtgga ggcgccgccg tggttccggg
      361 tgaccgcgta ggcgacccca cccccgacca gggcgagcgc catgatcccg gcgaccaccc
      421 agccgagccg ctccctggcc agagtcttga acacactctt tatcctgccc atgacgggtc
      481 aggatatcgc gacctcggcg attcggcggc gcaggtaccg ctgttcgact ggattcgtcg
      541 ccaggtcgag ggcgcggcgg taggcggctc ccgccgcctc cagccgcccg agccgccgca
      601 gcaggtcggc gcgggtcgcg tggagcaggt ggtagtcctc cagctcgccg ccggcggcca
      661 gctcctcgac cagggcgagg ccgcacatcg gaccgtcggc catggcgacc gcgaccgcat
      721 ggttgagcgc caccaccggc gacggaagca gcgccccgag ccgctcgtac agggccgcga
      781 tgcggcgcca gtcggtcgcg tcggcggccg gcgcggtcgc gtgcagcgcg gcgacgcacg
      841 cctgcagccg gtacggcccg ggcgggccca tccgcatcgc ccggtcgagc agccggacgc
      901 cctccccaat ctcctcgtgg tcccaacggg tgcggtcctg gtcctccagc agcaccaggt
      961 cgccgtccgg gccggtgcgt gtctggcgcc gggagtcctg caacagcagc agcgccagca
     1021 ggccaagggc ctccggctcg tcgggcatga gcgcggccag caggcggcag agccggatcg
     1081 cctcgccgct cagctcgcgc cgcaccaggt cgtccccggc gctggctgaa tagccctcgt
     1141 tgaagaccag gtagagcacg gcaaggaccg cgccgagccg ttccggcagc aggtggtcgg
     1201 gcggcacccg ataggggatg ccagcgtcgc ggatcttgcg cttggcccgc accagccgct
     1261 gcgccatggt cgcctccggg accaggaagg catgggcgat ctccggcgtg gtgaggccgc
     1321 cgagggcgcg aagggtgagc gccacccgcg cctccagcga cagcgccggg tggcagcagg
     1381 tgaagatcag ccgcagccgg tcgtcgccga tggtgtgcgt ctcctcctcc gcctcctggc
     1441 gttccgcttc cagcagcgcc agcgcgggct gccgttcggc gaacccgcgc tcccggcgca
     1501 gccggtcgat cgccttgttg cgggcggtgg tggtgatcca ggcgcccggc cgtggcggga
     1561 ccccgtcgac cggccagcgc tcacaggcga ccaggaacgc ctcggccacc gcctcctcgg
     1621 cccggtcgaa gtcgccgagc tggcggatga gggtcgcgac ggccttcccg gactcctccc
     1681 ggaacacccg ctggaccgcc tcgcggacct ctgtcgcgac cctcgtcaca ccgcctcgct
     1741 tgatgccctg gacttcggct ggcctcagct gaccggtggg atctccatga tcggccgcac
     1801 ctcgatcgag ccgctcttgg cgccggggat cttggccgcg gtcgcgatcg cctcgtcgag
     1861 atccctgcag tcgaccaggt agaagccgcc gagctgctcc ttcgtctcgg cgaacgggcc
     1921 gtcggtggtg agcgtctcgc cgttgcgcac ctgcaccgtg gtggcggtgc tggtcggctg
     1981 cagggcctcg cctccctgca tcagccctcg gtccacgatc tccttggtga aggagttgta
     2041 ggcgtcgaac tcggcctgcg tctccgcctc agtcgcggtg gccatggccg cctcggaccc
     2101 gtagataagc agcgcgtacc gcatctgcgc ctccctttcc ctgtgtccgg cggcaccgtc
     2161 gccgccctct accaagacaa cgatcgggag tggcgg
//
LOCUS       DASWDC010000154         6535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1288411, whole genome shotgun sequence.
ACCESSION   DASWDC010000154 DASWDC010000000
VERSION     DASWDC010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288411"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VF995_04910"
     CDS             complement(<1..663)
                     /locus_tag="VF995_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017541643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX9342937.1"
                     /translation="MVFQVDSEVGVLRQVIVHRPDLELKRLTPSNKDEFLFDDVLWVK
                     RAKEEHDGFAEALRERGVRVHRFDELLRETVADPVARKHVLDRVLDEHLYGPLAIDAM
                     RAAFDAMDDDTLTEFLIGGATKRELLERAEAPRSLVFHTIGMDDFLLAPLPNHLFTRD
                     ASCWIGGGVAINSMRKKARRRETIHYEAIYRWHPLFSSQDFGIWSQGTEGGVASVEGG
                     DVL"
     gene            741..2192
                     /locus_tag="VF995_04915"
     CDS             741..2192
                     /locus_tag="VF995_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9342938.1"
                     /translation="MDRAQLIRRLAEAVGPGGIITERNQLRTYECDGLAAFRVIPSVV
                     VLPETTEQAQGVVRVCREAGVPFVARGSGTGLSGGALPLADGVLIVLARMRRVVEVDL
                     PNQRVVVEPGVLNLQVTKEVAPHGYYYAPDPSSQQVCSIGGNVAENSGGAHCLKYGFT
                     TNHVTGLELVLPDGELAHLGGKAPDPPGYDLIGAFVGSEGTLGIATKVTVRILRKPEA
                     VRTLLAAFDGTDDAGEAVSGVIGAGIVPAAIEMMDALAIEAAEAAVHPNYPDAGAVLI
                     VELDGPAPEVEVQFNQVEAICRDSGATELRVAADDEERALFWKGRKSAFAAVGRISPD
                     YYVQDGVIPRTALPEVLREISRMAAERGLRVANVFHAGDGNLHPLVLYDAAVEGQEQR
                     AEELSAEILFVCLRHGGSITGEHGVGFDKKQYLPRQFSADDLDTMQLVRCAFDPDSRS
                     NPGKVFPTPRLCGERPGPRRVHPVEQAGLAEVF"
     gene            2193..3524
                     /locus_tag="VF995_04920"
     CDS             2193..3524
                     /locus_tag="VF995_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9342939.1"
                     /translation="MAFELPGGVASRPATEADAVDGVQPELVAEPATVEEAAAVLREA
                     TAAGKRVVCRGAGTKLGWGNPPTGAEIILSSARLDRILEHAEGDLIVKAEAGVPLEAL
                     QAAVGEAGQLLALNPPSAGGGATLGGIVAANASGSWRLRYGTVRDLLIGITVVLADGT
                     IAHAGGKVVKNVAGYDLGKLFTGSLGTLGLIAEVTFRLHPRTPDRATTRLRLEPDRPE
                     QAGAAVSAILGSQLVPTTLDLHWPDPAGHGTLLLSFEGTAPGPVDQAKAAAALLGLGE
                     GEGPGERPGEGPGKNGTPPPEGGPPDFNLSAREPWLPRLSTGKASETGVLVKAACAPS
                     ELPGAIRDLWEAAGTHGLRAGLAGQPGSVVLYATLDGGGADALVGAVEEARKRLLGRG
                     GSLVVLGAPLEVKRRLDVWGPVGDALPLMRRVKERFDPNGTMSPGRFVGGI"
     gene            3526..4875
                     /locus_tag="VF995_04925"
     CDS             3526..4875
                     /locus_tag="VF995_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX9342940.1"
                     /translation="MAEPAAGTGPGPAPAFDLHHPPDKVLIDDCVHCGFCLPTCPTYV
                     LWGEEMDSPRGRIYLMDIGLEGEPMNDEMVRHFDQCLGCMACVTSCPSGVQYDKLIEA
                     TRAQVARRHPRPWRERAFRGLLFKLFPYPRRLAALRGPLRLWQRSGLGRLAARARVLP
                     DGLASMERLLPDLPEARAPLPEITAAVGERRRRVGMLTGCVQRVFFPQVNAATARVLA
                     ADGCEVAAPSTQGCCGALSMHAGREAEAQRFAKALIAVFEGAGVDQVVVNVAGCGSTM
                     KEYGHLLRDDPAWAERAAAFSARVRDFSELLSELGPAAERHPLPLTVAWHDACHLAHA
                     QGVRAQPRQVLRAIPGLEVRELAEAEICCGSAGIYNLVEPDPARELGVRKAANVRASG
                     ADLLATANPGCLLQIRASLDALDANGAGLPMAHLAEVVDASIRGLPPEALGPASARP"
     gene            complement(4872..5129)
                     /locus_tag="VF995_04930"
     CDS             complement(4872..5129)
                     /locus_tag="VF995_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342941.1"
                     /translation="MWEYVIREFQQEWGDPSEARAANAFVAELNELGREGWEAVGMSP
                     RTHYDRGGGPPGWDSFTFLVLLKRRVTAKQPAEREVESASV"
     gene            complement(5211..5708)
                     /locus_tag="VF995_04935"
     CDS             complement(5211..5708)
                     /locus_tag="VF995_04935"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9342942.1"
                     /translation="MRAAILALLAERSMHGYEMIQELQARTGGVWRPSPGSVYPTLQM
                     LEDEGLISSEEAEGRRRFTLTDTGRAEADRLATPQKPWEEVTEGAGPTVMRVGIAAAQ
                     VRDAVVQIFRSGTDAQQAKAIEVLADTRRRLYAILAEDGDEDGDEDGDDAGIDSDTGT
                     GAGEA"
     gene            complement(5968..6468)
                     /locus_tag="VF995_04940"
     CDS             complement(5968..6468)
                     /locus_tag="VF995_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342943.1"
                     /translation="MSVISPPPTRSRVLPIAWVLLLLLGLFFLIAPAADLAGDARQGL
                     PTDHQTTFTKVAGTSFASVRQSVPGTARYVTLLERGYALHELTFGLLFLAIVAVPFRR
                     RERWAWWACWAVMVANLGYTLTFGRHDQTVLWRSLIGDVALPVLLLVTIPAFFFHPRE
                     AARAAS"
BASE COUNT          910 a         2362 c         2333 g          930 t
ORIGIN      
        1 cagcacgtcg ccgccttcga cggaggcgac cccgccctcg gtgccctgcg accagatgcc
       61 gaagtcctgg ctggagaaca gcgggtgcca gcggtagatc gcctcgtaat ggatggtttc
      121 acgcctgcgg gccttcttgc gcatggagtt gatcgccacg ccgccgccga tccagcagga
      181 ggcgtcgcgc gtgaagaggt ggttggggag cggcgcgagc aggaagtcgt ccatgccgat
      241 cgtgtggaac accagcgaac gcggcgcctc ggcacgctcc agcagctccc gcttggtcgc
      301 cccgccgatg aggaattcgg tcagggtgtc gtcgtccatg gcgtcgaagg cggcccgcat
      361 ggcgtcgatc gccagcgggc cgtagagatg ctcgtcgagg acccggtcga ggacgtgctt
      421 gcgtgccacc gggtcggcga ccgtctcgcg cagcagttcg tcgaagcggt ggaccctgac
      481 cccgcgttcc cgcagcgcct cggcgaaccc gtcgtgctcc tccttggcgc gtttgaccca
      541 gagcacgtcg tcgaacagga actcgtcctt gttgctcggg gtgagccgtt tcagctccag
      601 atcgggccgg tgcacgatca cctgacggag gaccccgacc tccgaatcaa cctggaacac
      661 cacaatcccc cccgcatctg ggcgccacca ttgacccctg cccgagtgca tcggcaccat
      721 ctggggtgga ggtgagggcc gtggaccgtg cccagctcat ccgccgcctg gccgaggcgg
      781 tcggccccgg cgggatcatc accgagcgca accagctccg cacctacgag tgcgacgggc
      841 tggccgcctt ccgggtgatc ccaagcgtgg tggtgctgcc cgaaaccacc gagcaggccc
      901 agggggtggt cagggtctgc cgcgaggccg gcgtcccgtt cgtggcgcgc gggtcgggca
      961 cggggctgtc cggcggcgcg ctgccgctgg ccgacggggt gctgatcgtg ctcgcccgga
     1021 tgcgccgcgt ggtcgaggtg gacctcccca accagcgcgt ggtggtcgag cccggggtcc
     1081 tcaacctgca ggtcaccaag gaggtcgccc cgcacggcta ctactacgcc cccgacccct
     1141 ccagccagca ggtctgctcg atcggcggca acgtggccga gaactccggc ggcgcccact
     1201 gcctcaaata cggcttcacc accaaccacg tcaccggcct ggagctggtg ctgcccgacg
     1261 gggagctggc ccacctcggc ggcaaggccc cggacccgcc cggctacgat ctgatcggcg
     1321 cgttcgtcgg ctcggagggc accctcggca tcgccaccaa ggtgaccgtg cggatcctgc
     1381 gcaagccgga agccgtccgc accctcctcg ccgccttcga cggcaccgac gacgccgggg
     1441 aggcggtctc cggcgtgatc ggcgccggga tcgtgcccgc cgctatcgag atgatggacg
     1501 cgctggcgat cgaggcggcc gaggcggccg tgcaccccaa ctaccccgac gcgggcgcgg
     1561 tgctgatcgt cgagcttgac ggcccagccc ctgaggtcga ggtgcagttc aaccaggtcg
     1621 aggcgatctg ccgggacagc ggcgccaccg agctccgcgt cgccgccgac gacgaggagc
     1681 gcgccctgtt ctggaagggt cgcaagtccg ccttcgcggc cgtgggcaga atctcccccg
     1741 actactacgt gcaggacggc gtcatcccgc gcacggcgct gccggaggtg ctgcgagaga
     1801 tcagccgcat ggccgccgaa cgcggcctgc gggtcgccaa cgtcttccac gcgggcgacg
     1861 gcaacctgca ccccctggtt ctctacgacg ccgccgtcga agggcaggag cagcgcgccg
     1921 aggagctttc cgccgagatt ctgttcgtct gcctgcgcca cggcggctcg atcaccggcg
     1981 agcacggcgt cggcttcgac aagaagcagt acctgccgcg ccagttcagc gccgacgacc
     2041 tggacaccat gcagctggtc cgctgcgcct tcgacccgga ttcgcgctcc aatcccggca
     2101 aggtgttccc caccccgcgc ctctgcggcg aacggccggg cccccgccgc gtccacccgg
     2161 tcgagcaggc cggcctcgcg gaggtgttct aggtggcctt cgagctgccg ggcggggtcg
     2221 cgtcccgccc ggccaccgag gccgacgcgg tcgacggcgt ccagcccgag ctggtcgccg
     2281 agcccgccac cgtcgaggag gccgccgcgg tcctgcgcga ggcgaccgcg gccggcaagc
     2341 gggtcgtctg ccggggcgcc ggcaccaagc tcggctgggg caacccgccg accggcgcgg
     2401 agatcatcct ctcaagcgcc cgcctggatc ggatcctgga gcacgccgag ggtgacctga
     2461 tcgtcaaggc cgaggccggc gtgcccctgg aggcgttgca ggcggccgtc ggcgaggccg
     2521 ggcagctgct cgcgctcaat cctccgagtg ccgggggcgg cgcgaccctc ggcgggatcg
     2581 tcgccgccaa cgcctccggg tcctggcggc tgcgctacgg caccgtccgg gacctgctga
     2641 tcggcatcac cgtggtgctc gccgacggca ccatcgccca tgcgggcggc aaggtggtca
     2701 agaacgtcgc cggctacgac ctcggcaagc tgttcaccgg ttccctgggg accctcggcc
     2761 tgatcgccga ggtcaccttc cgcctgcacc cgcggacccc cgaccgcgcc accacgcgcc
     2821 tgcggctgga gccggaccgc cccgagcagg ccggcgcggc ggtcagcgcc atcctcggct
     2881 cgcagctggt cccgaccacc ctcgacctgc actggccgga cccggccggc cacggcaccc
     2941 tgctgctcag cttcgagggc accgccccgg gtcccgtcga ccaggccaag gcggccgccg
     3001 cactgctggg cctcggtgag ggcgaaggcc ccggcgaacg ccccggcgaa ggccccggga
     3061 aaaatggcac accgccgcca gaggggggtc cccctgattt caatctatca gcgcgcgaac
     3121 cgtggctgcc gaggttatcc acaggcaaag ctagcgaaac gggcgtactg gtgaaagcgg
     3181 catgcgctcc cagtgagttg cccggcgcga tccgcgacct gtgggaggcg gcgggtacgc
     3241 acgggctgcg tgccggcctt gccgggcagc ccggcagcgt ggtgctgtac gccacacttg
     3301 acggcggcgg cgcggacgcc ctggtcggcg cggtcgagga ggcccgcaag cggctgctcg
     3361 ggcgcggcgg gtcgctggtc gtgctcggcg cgccgctgga ggtgaagcgg cggctcgacg
     3421 tgtggggacc ggtcggcgac gcgctgccgc tgatgcggcg ggtcaaggag cgcttcgacc
     3481 cgaacggcac catgagtccc ggacgcttcg tgggagggat ctgacatggc cgaacccgca
     3541 gccggaacgg gccccggacc cgcacccgcg ttcgacctgc accatccgcc ggacaaggta
     3601 ctgatcgacg attgcgtcca ctgcgggttc tgcctgccga cctgcccgac ctacgtgctg
     3661 tggggcgagg agatggactc gccgcgcggg cgcatctacc tgatggacat cgggctcgaa
     3721 ggcgagccca tgaacgacga gatggtccgc cacttcgacc agtgtctcgg ctgcatggcc
     3781 tgcgtcacct cctgcccgtc gggggtccag tacgacaagc tgatcgaggc cacccgggcg
     3841 caggtcgcgc ggcgccaccc gcggccctgg cgcgagcggg ccttccgggg gctgttgttc
     3901 aagctgttcc cctacccgcg gcggctcgcc gcgctgcgcg ggccgctgcg cctctggcag
     3961 cgttccgggc tcggccggct ggccgccagg gcgcgcgtgc tgccagacgg actcgcgtcg
     4021 atggagcgcc tcttgccgga cctgccggag gccagggcgc cgctcccgga gatcactgcg
     4081 gcggtggggg agcgccgccg ccgcgtcggc atgctcaccg ggtgcgtcca gcgagtcttc
     4141 ttcccgcagg tcaacgccgc caccgcgcgg gtgctcgccg ccgacggctg cgaggtggcc
     4201 gcaccctcaa cccagggctg ctgcggcgcg ctcagcatgc acgccggccg cgaggccgag
     4261 gcacaacgct tcgccaaggc cctgatcgcg gtcttcgagg gcgccggggt cgaccaggtg
     4321 gtggtgaacg tggccggctg cggctccacc atgaaggagt acgggcacct gctgcgcgac
     4381 gacccggcat gggcggagcg tgccgccgcc ttctcggcca gggtccgcga cttctccgag
     4441 ctgctctcgg agctcggccc ggccgccgag cgccacccgc tgccgctcac ggtcgcctgg
     4501 cacgacgcct gccatctggc ccacgcccaa ggcgtccgcg cgcagccccg ccaggtcttg
     4561 cgggcgatcc ccgggctgga ggtgcgggaa ctggccgaag ctgagatctg ctgcggctca
     4621 gccggcatct acaacctggt cgagcccgat ccggcccggg agctcggcgt gcgcaaggcc
     4681 gccaacgtgc gcgccagcgg cgccgacctg ctcgccaccg ccaatcccgg ctgcctgctg
     4741 cagatccgcg ccagcctcga cgcactggac gccaacggcg ccggcctccc catggcgcat
     4801 ctcgccgagg tggtcgacgc ctcgattcgt ggcctgccgc cggaggcgct cggcccggcc
     4861 tcggcccggc cctagaccga ggcgctctcg acctcccgtt cggcgggctg cttcgcggtg
     4921 acgcggcgtt tcagcagcac caggaaggtg aagctgtccc agccgggagg gccaccgccg
     4981 cgatcgtagt gggtgcgggg cgacatcccg accgcctccc aaccctcccg gcccaactcg
     5041 ttcagttcgg cgacgaaggc gttggcggcg cgtgcctcgg atgggtcgcc ccactcctgc
     5101 tggaactcgc ggatcacgta ctcccacacg gtggatttcc tctccttggg accggtgacc
     5161 gaaaagtcta cctcggtacg gccccaagga gagcggggcg ctggcggggc tcacgcctcg
     5221 ccagcgccgg tgccggtgtc gctgtcgata ccggcgtcgt cgccgtcctc gtcgccgtcc
     5281 tcgtcgccgt cctcggcgag gatcgcgtag aggcggcggc gggtgtcggc gaggacctcg
     5341 atggccttcg cctgctgggc gtcggtgccg ctgcggaaga tctgcacgac ggcgtcgcgt
     5401 acctgggcgg cggcgatgcc gacgcgcatc acggtcgggc ctgcgccctc cgtgacctct
     5461 tcccacggct tctgcggggt tgccaggcgg tccgcctcag ccctgccggt gtcggtcagg
     5521 gtgaagcggc gccggccctc ggcctcctca ctggagatga ggccctcgtc ctccagcatc
     5581 tggagggtgg ggtagaccga gcctgggctg ggtcgccaca cgcctccggt gcgggcctgg
     5641 agttcctgga tcatctcgta gccgtgcatg gaccgttcgg ccagcagcgc gaggatcgcg
     5701 gcgcgcacgt tgccgcgccg tgccccccgc ccgcggccgt gccgtccggg gaagggcccg
     5761 ggaccgggac cgtgcccgtg cccgtggtgg cctgggccgc cgaacggtgg gccccagggg
     5821 ccgaacgccg ctcgcgcgtc agaccaggct tcccggaggc ctctgactcg tggatctcgt
     5881 gcatctcgat gcatctgtga tcacctcaca atcggttctc gtgaggtaac gatatatcga
     5941 gatgcatcgc gatgcaacat aaatctttca gctggccgct cgggccgcct ctcgggggtg
     6001 gaagaagaag gctggaatgg tcaccagcag caggaccggc agggcgacgt cgccgatcag
     6061 gctgcgccac agcaccgtct ggtcgtggcg gccgaaggtc aaggtgtagc cgaggttggc
     6121 gaccatcacc gcccagcagg cccaccaggc ccagcgttcc cggcgccgga acgggacggc
     6181 gacgatcgcc aggaacaaca gcccgaaggt cagctcgtgc agggcgtagc cgcgctccag
     6241 cagggtgacg tagcgggcgg tccccgggac ggactgcctg acgctcgcga agctggtccc
     6301 ggcgaccttg gtgaaggtcg tctggtggtc ggtgggaagg ccctggcgcg cgtcgccggc
     6361 gaggtccgcg gcgggcgcga tcagaaagaa gaggccaagc agcaagagca gcacccaggc
     6421 gatcggcagc acccgggacc tggtcggagg cggggagatg acggacatct aaatgcttcc
     6481 tgggagtggt tgcatgtgct ggctggtagg gggcatggtc acctctccgg agggc
//
LOCUS       DASWDC010000155         6611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1289048, whole genome shotgun sequence.
ACCESSION   DASWDC010000155 DASWDC010000000
VERSION     DASWDC010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289048"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VF995_04945"
     CDS             complement(<1..551)
                     /locus_tag="VF995_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX9342944.1"
                     /translation="MPDAIDPKRAALIIQDLQNDVIHERGAFADSGSPAHARSQNVVG
                     NVQGLADACRRVGTPVIHVWYIVDPGAAGLKQNAPLYRAVKDTGGLVRGSWGAAPVDG
                     LEAREGDFVVEKLRMNAFYDTRLDALLRGFGVETLIICGAWTNMSIEHTARHAADAGY
                     EVVVCSDGTSTISEEWHHAGLNYA"
     gene            complement(617..1531)
                     /locus_tag="VF995_04950"
     CDS             complement(617..1531)
                     /locus_tag="VF995_04950"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9342945.1"
                     /translation="MGVTVKSDVLLELVEPGAEAEQLGTGFLFTEGPIWNPEEQVLYF
                     SDMPGDVRRRWSEQDGVGEVMRPSNKGNGMTYDAELRLVVCEHVTSSVTRFPRGSVAG
                     DHETLASHYQGKELNSPNDVCVRSDGSIWFSDPTYGRMPVFGLEREQDLDFQGFYRIP
                     PGGGAPELVKGDFLQPNGLCFSPDESLLYVNDSDRAHIRAFDVATDGSVSNDRVFAEG
                     IGKAILEEGLADGMKCDQRGDVWVTGPGGIWVFSPEGEHLGVVEIPEVVGNLHWGGPD
                     WSDLFVCASTSLYRVRTRTRGRHEPFMG"
     gene            1774..2559
                     /locus_tag="VF995_04955"
     CDS             1774..2559
                     /locus_tag="VF995_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9342946.1"
                     /translation="MTTLRDVQVALPASPAEAVAAFGDGSGVTVIAGGTIAVPELNAG
                     RLRPARAILLGRAGMDGITRTAGVVRIGAACPLAVLEAGVPDPLAAAAREVGDQEIRG
                     QGTLGGNLCAPPADDVQRGDLRAPLIALAAQIRSAGAGGERTGPVEDFLSSASSRTRL
                     LLEVEFEEPRWAGYASLHRAHAHHYTVLAVSAAETSAGLRIAVTGAGGAGVRLRAAEA
                     SGDPDDAVGDVEFADDALASAWYRRRMLPVLVARALATRTRSS"
     gene            2594..3103
                     /locus_tag="VF995_04960"
     CDS             2594..3103
                     /locus_tag="VF995_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9342947.1"
                     /translation="MKLTVNGVDHEIASAPLTPLLHVLRDELDVTSPKAGCQQGGCGT
                     CTVLVDGEPRRSCLLAVAMVEGHEVTTVEGLTPAGGLSPLQRAFHELGGVQCGFCTPG
                     MIVAAHALLANAGRAVEREEVIEALGGHYCRCTGYVKIVEAVQTASRSDGAAGQPALP
                     ASDTEEVRA"
     gene            3100..4461
                     /locus_tag="VF995_04965"
     CDS             3100..4461
                     /locus_tag="VF995_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9342948.1"
                     /translation="MKAVGARLPRYDGQGHVAGKTLYVDDVRVPGMLWAKALRAPVHH
                     AAVRRLDTSRAERMPGVAAVVTHADVPLNVYGHLEGLGVPADEPLLAAGEVRYQGQPI
                     AVVAATSEEAALEAVAAIEVEYEERPALFDIREAFDPDAPTIHPWGPVFPHFGPHNRR
                     RVRKGDVEAAFDAADVIVEGVYRPQAIEHVPMETQVAAAVPEPSGRLVIYSCTQAMYF
                     SMGVVAAHLKLPLNKLKFVGGTVGGGFGGKVDTAAETIAALLALKSSRPVKWRWTREE
                     EFLCSSTRAPWHMELADAVTADGWILGRKMLTLHDAGAYTRFSSYGVTKHAFHHTGAY
                     TIPNLHFDGFVVYTNRVPTTAMRGFGVTSVSFAVELQLSRIASVLGMDPWELRLKNAN
                     RVGDTTANWVALKDPSTVPVVQAAAQRVGVELAEPFRRMTAGRRDGELLPEHLVGQQG
                     GGG"
     gene            4463..5563
                     /locus_tag="VF995_04970"
     CDS             4463..5563
                     /locus_tag="VF995_04970"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9342949.1"
                     /translation="MAKHRGRGVATIEYPTGMNQNGDPSQAWIKVKPDGRVDVFTGTS
                     EIGGGEKTVQAQIVAEALGVPYEWVTIDNSNTDSSPVCTGTFASRATFIAGNAVRFAA
                     EKVRGRILDLAGDLLEVAAEDLEIVEGEVQLKGAPDRRISVPEVAGAATYQYGELLSA
                     SGTQLKPFAPVDDDTGRVELEPHSAISYAACVADVEVDDETGAVTVLRLVQVYDVGRA
                     INPMLVEGQIEGGAMMGLGLGLLEQAYPYYPSVAHRGGQFGGYLAPAMADLPEFDDVI
                     LEHASTDGPFGAKAIGEMANNAQPPAIMAAIHDAVGVWVTEAPATPERVLRALDARAE
                     PRRDGKRVIFDEDASVRTVSSDGGRGFLADGS"
     gene            5553..5945
                     /locus_tag="VF995_04975"
     CDS             5553..5945
                     /locus_tag="VF995_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9342950.1"
                     /translation="MARSPFLSWNGVDPWQIYPGVHLHAAGGERVLLCRVTYAPGTDV
                     ARHTHEASEQLMVITDGSVEVTIGTETRKLEAGDACVITRGIEHELHSAEGATFFEAL
                     SPVPLDHVPDRDRDLVLGADGDTLHVAR"
     gene            complement(6001..>6611)
                     /locus_tag="VF995_04980"
     CDS             complement(6001..>6611)
                     /locus_tag="VF995_04980"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX9342951.1"
                     /translation="DSRQVHALLMDALGSDVQGDFFAFAVSPPRPLQADRAGRSGWRF
                     PIEARLAARTFVAVRLEVVARAEEIRGGTEPLTFASSLAFASYPPSVTVEAVDLAQHA
                     AEKFHALTRTYGERPNTRVKDLVDLVLLIENEMLDPARLRERLAIVFSARGTHQMPDD
                     LPAPPAAWERDYAALVADLDIEARTVNAALDRVRPVWRAYVI"
BASE COUNT          969 a         2473 c         2238 g          931 t
ORIGIN      
        1 gcgtagttga ggccggcgtg gtgccactcc tcgctgatgg tggaggtgcc gtcggagcag
       61 accaccacct cgtagccggc gtcggcggcg tggcgggcgg tgtgctcgat cgacatgttg
      121 gtccacgcgc cacagatgat cagcgtctca acgccgaagc cgcgcagcag cgcgtcgagg
      181 cgggtgtcgt agaaggcgtt catgcgcagc ttctcgacga cgaagtcgcc ctcccgcgcc
      241 tccaggccgt cgaccggcgc tgccccccag ctgccccgca ccagcccgcc ggtgtccttg
      301 acggcgcggt acagcggcgc gttctgcttc agcccggcgg cacccgggtc cacgatgtac
      361 cagacgtgga tcaccggggt gccgacccgg cggcaggcgt cggcgaggcc ctggacgttc
      421 ccgaccacgt tctggctcct ggcgtgcgcc ggggagccgg agtcggcgaa ggccccgcgc
      481 tcatggatga cgtcgttctg gaggtcctgg atgatcaggg cggcgcgctt ggggtcgatg
      541 gcgtccggca ttggggctcc tcctcgtgct cgtccgcagg tctccgtggg tctccgcagg
      601 tcgtcgtgcc ttcgcctcag cccatgaacg gttcgtgcct gccgcgggtc ctcgtccgca
      661 cccggtacag cgaggtggag gcgcagacga acaggtcgct ccagtccggg ccgccccagt
      721 ggaggttgcc gaccacctcg gggatctcga ccacgccgag atgctcgccc tcggggctga
      781 acacccagat cccgccgggg ccggtcaccc agacgtcgcc gcgctggtcg cacttcatcc
      841 cgtcggccag gccctcctcc aggatcgcct tgccgatccc ctcggcgaag acgcggtcgt
      901 tcgacaccga cccgtcggtg gcgacgtcga aggcgcggat gtgggcgcgg tcggagtcgt
      961 tgacgtacag cagcgactcg tcgggcgaga agcagagccc gttcggctgc aggaagtcgc
     1021 ccttgaccag ctccggcgcg ccgccgcccg gcgggatgcg gtagaagccc tggaagtcga
     1081 ggtcctgctc ccgctccagc ccgaacaccg gcatgcggcc ataggtcggg tcgctgaacc
     1141 agatcgaccc gtcggagcgg acgcagacgt cgttggggct gttcagctcc ttgccctggt
     1201 agtgcgacgc gagcgtctcg tgatcgccgg cgaccgagcc gcgcgggaag cgagtcacgc
     1261 tggaggtgac gtgctcgcag accaccaggc gcagctccgc gtcgtaggtc atgccgttgc
     1321 ccttgttgga gggccgcatc acctcgccga cgccgtcttg ttcgctccag cggcggcgca
     1381 cgtcgccggg catgtcgctg aagtagagca cctgctcctc ggggttccag atgggcccct
     1441 cggtgaacag gaacccggtg ccgagctgct ccgcttcggc cccgggttcg accagttcga
     1501 ggagtacgtc gctcttcacg gtgaccccca tcggctctcc tctcgttgac gacgcgtcca
     1561 cctcgacacg tcacaaggac acaaatccga cagtgctcac taccctggtg gatcggttgt
     1621 cgctggtcac ctgctgtgaa atgccccacc gagagggtcg ctcacggccc cggactaact
     1681 tgctgcgaag cagacctttt gtcaatgaga catcaattca cgtatatgaa ccaacgattg
     1741 acaagggcgc accaccctgc gacaactcag cgcatgacga cacttcggga cgtccaggtc
     1801 gcgctccccg cctctccggc tgaggcggtc gcggcgttcg gggacgggag cggcgtcacc
     1861 gtgatcgcgg gcggcaccat cgccgtcccg gagctcaacg ccgggcggct gcgcccggcc
     1921 cgggcgatcc tgctcggccg cgccgggatg gacggcatca cccgcacggc cggggtcgtc
     1981 cgtatcggcg ccgcctgccc gctcgctgtc ctggaggcgg gggtgcccga cccgctcgcc
     2041 gccgccgccc gcgaggtcgg cgaccaggag atccgcgggc agggcaccct cggcggcaac
     2101 ctctgcgcgc cgcccgccga cgacgtccag cgcggcgacc tgcgcgcccc gctgatcgcc
     2161 ctggccgccc agatccgctc ggccggcgcg ggcggcgagc ggaccgggcc ggtcgaggac
     2221 ttcctcagct ccgcctccag ccgaacccgg ctgctcctgg aggtcgagtt cgaggagccg
     2281 cgctgggccg gctacgcctc gctgcaccgc gcccacgccc accactacac cgtcctcgcc
     2341 gtctccgccg ccgagacctc ggcgggactc cgcatcgccg tcaccggcgc gggcggcgcc
     2401 ggggtgcgcc tccgcgccgc cgaggcgagc ggcgaccccg acgacgccgt cggtgacgtc
     2461 gagttcgccg acgacgccct cgcctccgcc tggtaccgcc gccggatgct gccggtgctg
     2521 gtcgccaggg cgctcgccac ccggacccgg agcagctgac tctcaaccga cggacccgac
     2581 gaaggggcgg ccgatgaagc tcaccgtcaa cggcgtcgac cacgagatcg cctcggcacc
     2641 gctcaccccg ctgctgcacg tcctgcggga cgagctggac gtcaccagcc ccaaagccgg
     2701 ctgccagcag ggaggctgcg gcacctgcac cgtgctggtg gacggcgagc cgcgccgctc
     2761 ctgcctgctc gccgtcgcca tggtggaggg ccacgaggtg accacggtgg aggggctcac
     2821 gccggccggc gggctgtcgc cgctgcaacg cgccttccac gagctgggcg gggtgcagtg
     2881 cggcttctgc acgccgggga tgatcgtcgc ggcccacgcc ctcctcgcga acgccggccg
     2941 cgcggtcgag cgcgaggagg tcatcgaggc gctcggcggc cactactgcc gctgcaccgg
     3001 ctacgtgaag atcgtcgagg cggtccagac tgcctcccgg agcgacgggg ccgccggcca
     3061 gccggcactg ccggcatcgg acaccgagga ggtgcgggca tgaaggcggt cggtgcgcgc
     3121 ctgccccgct acgacggcca gggccacgtc gccggcaaga ccctctacgt cgacgacgtc
     3181 cgcgtcccgg ggatgctgtg ggccaaggcg ctgcgcgcac ccgtccacca cgccgccgtc
     3241 cgccgcctcg acaccagccg ggccgagcgc atgcccggcg tcgctgcggt cgtcacccac
     3301 gccgacgtgc cgctcaacgt ctacgggcac ctggagggcc tcggcgtccc cgccgacgag
     3361 ccgctgctcg ccgcgggcga ggtccgctac cagggccagc cgatagccgt ggtcgccgcg
     3421 accagcgagg aggccgccct ggaggcggtc gccgcgatcg aggtcgagta cgaggagcgc
     3481 ccggccctct tcgacatccg cgaggccttc gaccccgacg ctcccacgat ccacccgtgg
     3541 gggccggtgt tcccgcactt cggcccgcac aaccggcgcc gggtccgcaa aggcgacgtg
     3601 gaggccgcct tcgacgccgc cgacgtgatc gtggagggcg tctaccggcc ccaggcgatc
     3661 gagcacgtgc cgatggagac ccaggtcgcc gcggcggtgc cggagccgag cgggcggctg
     3721 gtgatctaca gctgcaccca ggcgatgtac ttctccatgg gcgtggtcgc cgcccacctc
     3781 aagttgccgc tgaacaagct caagttcgtc ggcggcaccg tcggcggcgg cttcggcggc
     3841 aaggtcgaca ccgccgccga gacgatcgcg gcgctgctgg cgctgaagtc cagccgcccg
     3901 gtgaaatggc gctggacccg cgaggaggag ttcctctgct cctccacccg cgcgccctgg
     3961 cacatggagc tggccgacgc ggtcaccgcc gacggctgga tcctcggccg gaagatgctc
     4021 accctgcacg acgccggcgc ctacacccgc ttctcctcct acggcgtcac caagcacgcc
     4081 ttccaccaca ccggcgccta caccatcccc aacctgcact tcgacgggtt cgtggtctac
     4141 accaaccggg tgccgaccac ggcgatgcgc ggcttcgggg tcacctcggt gtcgttcgcg
     4201 gtcgagctgc agctgagccg gatcgccagc gtcctcggca tggacccgtg ggagctgcgc
     4261 ctcaagaacg ccaaccgcgt cggcgacacc accgccaact gggtcgccct caaagacccc
     4321 tcgacggtcc cggtggtgca ggccgccgcc cagcgggtcg gcgtcgagct ggccgagccg
     4381 ttccgccgca tgaccgccgg gcgccgcgac ggcgagctgc tcccggagca tctcgtcggc
     4441 cagcagggag gtggtggctg agatggccaa gcaccgcggc cggggggtcg cgaccatcga
     4501 gtaccccacc ggcatgaacc agaacggcga cccgagccag gcctggatca aggtcaagcc
     4561 cgacgggcgc gtggacgtct tcaccggcac ctccgagatc ggcggcggcg agaagaccgt
     4621 ccaggcccag atcgtcgccg aggccctcgg cgtcccctat gagtgggtga cgatcgacaa
     4681 ctccaacacc gactcctcgc cggtctgcac cggcaccttc gcctcccgcg ccaccttcat
     4741 cgccggcaac gccgtccgct tcgccgccga gaaggtcagg ggccggatcc tcgacctcgc
     4801 cggggacctg ctggaggtcg ccgccgagga cctggagatt gtggagggcg aggtccagct
     4861 gaagggcgcg cccgaccgca ggatctcggt gcccgaagtc gccggcgccg ccacctacca
     4921 gtacggcgag ctgctcagcg cctccggcac ccagctcaag ccgttcgccc cggtcgacga
     4981 cgacaccggc cgggtcgagc tggagcccca ctcagcgatc tcctatgccg cctgcgtcgc
     5041 cgacgtcgag gtcgacgacg agaccggcgc ggtcacggtg ctccgcctcg tccaggtcta
     5101 cgacgtcggc cgcgccatca acccgatgct ggtcgaaggc cagatcgagg gcggggcgat
     5161 gatgggcctc ggcctcggcc tgctggaaca ggcctacccc tactacccgt cggtggcgca
     5221 ccgcggcggc cagttcggcg gctacctcgc ccccgccatg gccgacctcc ccgaattcga
     5281 cgacgtcatc ctggagcacg cctccacgga cggccccttc ggcgccaagg cgatcggcga
     5341 gatggccaac aacgcccagc cgccggcgat catggccgcc atccacgacg ccgtcggcgt
     5401 ctgggtcacc gaggcccccg ccacccccga acgggtcctg cgcgccctcg acgcccgcgc
     5461 cgaaccccgc cgcgacggca aacgggtgat cttcgacgag gacgcctccg tgcgaaccgt
     5521 ctccagcgac ggcgggaggg ggttcctcgc cgatggctcg tagccccttc ctctcctgga
     5581 acggcgtcga cccctggcag atctaccccg gcgtccacct ccacgccgcc ggcggcgaac
     5641 gggtcctcct ctgccgggtg acctacgccc cgggcactga cgtcgcccgc cacacccacg
     5701 aggccagcga gcagctgatg gtgatcaccg acggctcggt ggaggtgacc atcggcaccg
     5761 agacccgcaa gctcgaagcc ggcgacgcct gcgtgatcac ccggggcatc gaacacgaac
     5821 tccactccgc cgagggcgcg acgttcttcg aggccctctc ccccgtcccc ctcgaccacg
     5881 tccccgaccg cgaccgcgac ctggtcctcg gcgccgacgg cgacaccctc cacgtcgcgc
     5941 ggtagccggg cgcttccagg cgtcagccgg gcccttcgcc ttgacggccc agcattgagg
     6001 ctagatcacg tacgcacgcc acactgggcg cacgcggtcc agggcggcgt tcaccgtgcg
     6061 ggcctcgata tcgaggtcgg cgaccaaggc ggcgtagtcc ctctcccagg ccgccggagg
     6121 agcgggaagg tcgtcaggca tctggtgggt gccgcgggcg ctgaacacta ttgcgaggcg
     6181 ctcacgtagc cgtgctggat cgagcatctc gttctcgatg agtagcacga ggtcgaccag
     6241 gtccttgact cgagtgtttg gtcgctcacc gtaggtgcgc gtcagggcgt ggaacttctc
     6301 cgccgcatgt tgagcgaggt cgaccgcctc gacagtgacg gaaggcgggt agctggcgaa
     6361 cgccagactg gaggcgaaag tgagcggttc agtgccgcct ctgatctcct cggctctcgc
     6421 taccacctcc aagcggaccg ccacaaaggt tctcgccgcg agccgggcct cgatcgggaa
     6481 acgccaaccc gacctgccgg cacgatcggc ctgtagcggt ctgggcggag agacggcgaa
     6541 agcgaaaaag tccccctgaa cgtcactgcc gagtgcgtcc atgagtaggg cgtgcacctg
     6601 ccggctatcg t
//
LOCUS       DASWDC010000156         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1289426, whole genome shotgun sequence.
ACCESSION   DASWDC010000156 DASWDC010000000
VERSION     DASWDC010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289426"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VF995_04985"
     CDS             complement(<1..417)
                     /locus_tag="VF995_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342952.1"
                     /translation="MPKERPARNAFDALVVLAVLIASVWVVPTGFAGRARAEAKPAQS
                     ATATRTPAASATTAPSVTPVSTPTPGRPQGPVVAVVDLGGFAKGVAFGAGSLWAASSD
                     GRLLRINPASNTVAAEIPVDPDGSGPAGVAVGAGAVW"
     gene            complement(474..1124)
                     /locus_tag="VF995_04990"
     CDS             complement(474..1124)
                     /locus_tag="VF995_04990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006554288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX9342953.1"
                     /translation="MVVQTDAIAAYLGELTAAEDDALRHARERAAAGGMPPVSADIGA
                     FLRFAARWVGAQAAVEIGSGAGYSGIWLARGLNRKGLLTTIEADPEHQRLAKSSYEEA
                     GVAGRVRAIGGRALEVLPRLSDGGYDLAFLDALKSEYPQYLEHALRLVRPGGVIIADN
                     VLWSGRVASPRASDPDTEGLRLYGRRISEDDRLDSVILTVGDGLAVSRVRQVGDRS"
     gene            1282..1794
                     /locus_tag="VF995_04995"
     CDS             1282..1794
                     /locus_tag="VF995_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX9342954.1"
                     /translation="MFNVGIGEIAVIVVVCLLVFGPDRLPEMARQVGRFLGQLRLNTQ
                     GALDQLKSEAELKDVPLPDLRVGSLRSQARDYLHQLMDIEGQMADLERERAELKRSLE
                     GEIAEAKATANGNGNGNGNGTSAATSIAETTTGSEAEPGSGPDPISDPLSDPEPPVTW
                     EAAPVDPEAT"
BASE COUNT          273 a          700 c          720 g          241 t
ORIGIN      
        1 ccagacggcc ccggcgccga cggcgacccc ggcgggcccg gagccgtccg ggtcgaccgg
       61 gatctcggcg gcgacggtgt tggaggccgg gttgatgcgc agcagccggc cgtcggagct
      121 ggcggcccac agcgatcccg cgccgaaggc gacgcccttg gcgaacccgc cgaggtccac
      181 caccgccacc accggcccct gcgggcgccc aggcgtgggc gtcgagaccg gggtgacgct
      241 gggggcggtg gtggcggagg cggcgggcgt cctggtcgcg gtggcggact gggccggctt
      301 cgcctccgcc cgagcccgcc cggcgaagcc ggtcgggacg acccagacgc tggcgatcag
      361 gaccgccaag accaccagtg cgtcgaaggc gttgcgcgcc ggccgctcct tcggcatcct
      421 cgcctgtccc tccgcgccct acgctccgct ggtcgactcc gtgctccgga cggtcacgaa
      481 cggtcaccca cctggcggac ccgtgagacg gcgaggccgt cgccgacggt gaggatcacc
      541 gagtcgagcc ggtcgtcctc ggagatccgg cggccgtaga gccgcaggcc ctcggtgtcc
      601 gggtcgctcg cgcgggggct ggcgacccgg ccggaccaga gcacgttgtc ggcgatgatc
      661 accccgccgg gccgcaccag ccgcagcgcg tgctccaggt actgcgggta ctcgctcttg
      721 agcgcgtcga ggaacgccag gtcgtagccg ccgtcgctga ggcgggggag cacctccagg
      781 gcgcgcccgc cgatcgcgcg gacccggccg gcgaccccgg cctcctcgta gctggacttg
      841 gcgagccgct ggtgctccgg gtcggcctcg atggtggtga gcaggccctt cctgttgagg
      901 ccgcgggcca gccagatgcc cgaatagccg gcgcccgagc cgatctcgac cgccgcctgg
      961 gcgccgaccc agcgcgccgc gaaccgcagg aacgcaccga tatcggcaga gaccggcggc
     1021 atgccgccag cggctgcccg ttcgcgcgcg tgccgcaggg cgtcgtcctc cgccgcggtc
     1081 agctcaccca ggtaggcggc gatggcatcg gtctgcacga ccacaggcga ctcccttgcg
     1141 tacgacagcg ctccccaccc gcttcagcct agcggcaacc ggcgcgctgg aacatcggca
     1201 gtgagcagga gtggccgaag ggatggtccg gggcttaggc tgggcatcag tttcgttcgg
     1261 gggcctcgtt agggaggcgc tgtgttcaac gtcggcatcg gcgagatcgc ggtgatcgtc
     1321 gtcgtctgcc tgttggtgtt cgggcccgac cgactgccgg agatggcgcg gcaggtcggg
     1381 cggttcctgg ggcagctgcg cctcaacact cagggcgcgc tcgatcagct caagtctgag
     1441 gccgagctga aggacgtccc actgccggac ctgcgggtcg ggtcgctgcg ctcgcaggcg
     1501 cgcgactacc tccaccagct gatggacatc gaaggccaga tggccgacct ggagcgtgag
     1561 cgcgcggagc tcaagcgctc ccttgagggc gagatcgccg aggccaaagc caccgccaat
     1621 ggcaatggca atggcaatgg caatggcacg agcgccgcca cctcgatcgc cgagaccacg
     1681 accggcagcg aggccgagcc cggaagcggc ccggacccga tctccgaccc gctctccgac
     1741 ccggagccgc cggtcacctg ggaagccgcg ccggtcgacc ccgaagcgac ctgacgccga
     1801 ccacagcgtc ggcgaccggc ccggcgaggg gggcggctcc tcctcgacga cgtgagcagc
     1861 ggctacgtga gcagcggcag gcgcctgccg gccatgctgc gctgcggcgg gagcgcggcg
     1921 gcgatgcgga gcag
//
LOCUS       DASWDC010000157        10569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1039595, whole genome shotgun sequence.
ACCESSION   DASWDC010000157 DASWDC010000000
VERSION     DASWDC010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039595"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..602
                     /locus_tag="VF995_05000"
     CDS             9..602
                     /locus_tag="VF995_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX9342955.1"
                     /translation="MIDIGAYLATSAAAAASLAVRPGAGAIEQGARLVATSLAGGGQV
                     LFCGNGGSAADAQHLSAELMGRLLLERSAYRSVALTTDTSALTAIANDYSYAEVFARQ
                     VQGLGRAGDVLVAITTSGRSENVVRAAAAAKDRGLVVVAFLGPARSPLDQTADVALHV
                     DGGTAGLIQQGHITIGHALCGWVESYLARRQAPGSRH"
     gene            604..1209
                     /locus_tag="VF995_05005"
     CDS             604..1209
                     /locus_tag="VF995_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9342956.1"
                     /translation="MRPAVFLDRDGTLVEEVSYLHEPDKVALVDGALPALRRLADARF
                     ALVVVSNQAGVARGYYRERDVEAVHGRLQALLAAGGVALDGIWYCPHHPDGTVAGYAR
                     ACRCRKPGAGMLEAAAVALRLDLAASWLVGNNPSDVGAARAAGVAPLYVTTGRASGET
                     PPPGVAAFPTLAAATAAILSQRVGGSAGERAAELRPGPPWP"
     gene            complement(1540..2256)
                     /locus_tag="VF995_05010"
     CDS             complement(1540..2256)
                     /locus_tag="VF995_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9342957.1"
                     /translation="MTPRRRAPDSAQLLQVLRRSELFGGGEEAALQRLVQQASPVAYP
                     AGAFLMRQGEPAAHVYVLTAGEVAIISPARGGAEQVHSIIGPGELIGELALLSDGKRT
                     AGARATSPTLAWLIGREAFWAFLDTTPSASAALLRLVAARLADREALIDDLLSLDVKG
                     RLAKALLGLAERHGHTAADGSQVIALRLTHRDLAGMVGASRENVTRGLASFRKRGLIA
                     YDTSAIRLLDPEALRRLVLS"
     gene            complement(2253..5402)
                     /locus_tag="VF995_05015"
     CDS             complement(2253..5402)
                     /locus_tag="VF995_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9342958.1"
                     /translation="MVTVLFADLVGSTTLVESLDPEAARSLLDESFHRLAAEIRRCGG
                     TLEKYIGDAILAVFGFPVAHGDDAARAVRAALAMRAVLEEPARPQAAGTGPSLRLRIG
                     MATGEVVAVLRASDLRVAGDAVHTAARIQQAAEPGQILISTRTARAARDLVVAGPPRR
                     IVARGKQRPVEVVEVTGMTTVSQPVSLMVDREEDLPKLLQAVDQAAVLSRLVLLVGEA
                     GVGKTTLVRATAARLGDQVQVLWGRCLPDWQSLPFWPVREVLAAAAGVPATDPSGTLA
                     EAITALVTESWTGPGPEAATTAAAVCRLIGLESASGRRGGRHRERAAEHATAPSQVGA
                     RELAAALAGVLCRMAAHERTVIVMEDLHWASRDLLDVATILVSGGCPPHSELAFVGTT
                     RPDLPLDPAWLQHTGARRIDLECLPERFTAALLRGVLGGDVELSDGLVARVQEASQGN
                     PLFVKELGLALREADEAKVAQARLPIPDSLRALIAARLDRLPPSCKQVLCRAAVIGRW
                     FTQESLAALSLPGTGAGSDHDGRGDAVGDRLDADLTVLLESGLIEQVPDWVSGRPNSF
                     TFQHTLFREVAYELLPKATRSELHRRLADHIVTRSGHQAHELVLPELIAPHLVAAVRL
                     AGQIRTPTAGDQQLAARAVGACRLAAERLRDQEALASAAHMLDEALALVGVAGTPPEE
                     LAELWVRRGALRAAIGDPAAAIADLRAAAAAERPAPRAVAYTELSNLYGRLNEFDRSA
                     AAADRALADATTTGRADLLARALRAKAQEPYLTGNLRETERLLLDAHAHARDGGHPDL
                     AIEVQSTLLPVRLQLATPLATIREEALSLAAAARAGGRRNAEAGASFALGEVAALHGD
                     LELARRHYEHANQQRCTIGLTLQRLWSLLGLVQVAAGRGDAAAARRLAAEAIALTAKP
                     DGSADPEAELHYAEACLAGDQPDLEGAALAVARSWHCLQAGDVLSRARLQRTEARLAA
                     ATGRHTEAVALLERALATLNPTGYRLERLRTLVDLVAALHRAGNQHARRAAPLTAEAL
                     ALARAVGARRLVRELGATR"
     gene            complement(5597..5824)
                     /locus_tag="VF995_05020"
     CDS             complement(5597..5824)
                     /locus_tag="VF995_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342959.1"
                     /translation="MSTIDPDEGKDPQAAPGSSGTESLRGPWESEPDTLDPDDPAQPE
                     PGDDTADGDGDGEEEGEGAGSSMAPERGPWK"
     gene            6064..6843
                     /locus_tag="VF995_05025"
     CDS             6064..6843
                     /locus_tag="VF995_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020552981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9342960.1"
                     /translation="MTRIRIKVVSDIHGAAEALRRQAAGADALLVCGDLVNLLDYRTM
                     DGIAVELYGRETIARFVELRNAARFEEAGGVLRTAVAGREGEVIARVRADMRAQYEAV
                     FAAFPERTYLTHGNVDNVDLFQDLLRPGVRHLDAEAVDVDGMRVGFIGGGLPRGRTPH
                     LSEVTHEQFSAKVARLPRVDVLCAHMPPAIDDLRFDLAAGRFEPGSQALLDYVEEHQP
                     DYLYYGHVHQPRKSRLRVGRTRLVNVGYFRATGRMLDHPAD"
     gene            6910..7347
                     /locus_tag="VF995_05030"
     CDS             6910..7347
                     /locus_tag="VF995_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9342961.1"
                     /translation="MAEHAEGTIEVYGTPAEIMGVIAGFDAYPEWVGNMDEAEVLERD
                     RQGRATTVAFKLRTPVMNAAYTLAYRYEPDDLGVSWTYVEGSLQDLSGQYALEPLDDG
                     TTRVTYRLEVDLGMPLPGLLKRQAAKQIVRSALGDLKQRVESA"
     gene            7402..8580
                     /locus_tag="VF995_05035"
     CDS             7402..8580
                     /locus_tag="VF995_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX9342962.1"
                     /translation="MLFTGKGGVGKTTVAAATAVRAAALGHRTLVMSTDPAHSLADSF
                     EFELGSKPRQVADNLWAEQIDTQARLEANWREIQDYVITLLNWGGVDAVEAEELSVIP
                     GLDELFSLIDISRYQQQGAYDLLVVDCAPTAETLRLLSLPEALSWYIDRLFPSSRRIA
                     SVARPLLGRLGSLPPIASDAVFGAVERLYRNLERVRMLLAEPESSTVRLVVNAEKMVV
                     AEARRTYTYLSLFGYRVDAVVVNRLLPDDVTDPYFLKWKEIQAVHYRSITESFAPLPI
                     LPCRLFDDEMVGLDRLGEVAGAVYGERDPAAVLAHSQPMRVSKTKGGYALLLDLPFAE
                     RAELDVLRKGEELYVKVGAYKRNVLLPQALQPLDVVSATLRDSTLEVRFGAAPSSPSR
                     "
     gene            8717..8869
                     /locus_tag="VF995_05040"
     CDS             8717..8869
                     /locus_tag="VF995_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342963.1"
                     /translation="MGLAVLRQSRPEALEHLLKAGAELLLAVRALLDGAEGLRGSATL
                     QRIDIG"
     gene            8936..9955
                     /locus_tag="VF995_05045"
     CDS             8936..9955
                     /locus_tag="VF995_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9342964.1"
                     /translation="MGSAVGPSGRGTVPPEPPVPPDPERRQAIGIDIGGTKIAAGVVA
                     TGGEIIDRTLVPTPSVEDQEPTVAAIREVIDTLRTRHPTAEAIGVGAAGLVEWPGGRI
                     IWAPHNAYRQLPLRRLLYEGTGLPTVVDNDANTAAWAEACFGAGVGVGHVVLLTVGTG
                     IGGGLVLGGQLYRGATGLGAEVGHMTVDPDGDPCACGNLGCLEAMASGTALGRAGRRA
                     AAADPGGRLAQLAGAPDAVTGEVVFQAAQEGDPTARELFEQLGFWLGVGIASLVSLFD
                     PELVVIGGGLVATGDLLLEPARAAMERYTFARPHRELPPVVPALLGHEAGLVGAAALA
                     LGTRA"
     gene            9948..>10569
                     /locus_tag="VF995_05050"
     CDS             9948..>10569
                     /locus_tag="VF995_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342965.1"
                     /translation="MPDGGFGGHSPAAGSTESGFHGGRGAPRWVNPARRLRIGLTLPQ
                     FRRDASSTLATFDELWRLGYDGAFVFDHLWPLLQPTRPALEGWTLLATLAARRGGGDG
                     FRLGTLVTRAGLRAPSLLAHMAATVRSIAGAPLIAGVGAGDAASRAENLAFGLPYEDA
                     AGRLANVEAAAVALAGTGAAEVWIGGLGATTRALAGRVADAWNAWGV"
BASE COUNT         1348 a         3884 c         3905 g         1432 t
ORIGIN      
        1 gattcagcgt gattgatatt ggagcttatc tcgcgacctc ggccgcggcg gcggcgtcgc
       61 tcgcggtccg gccgggcgcg ggcgccatcg agcagggcgc gcgcctggtg gcgacctccc
      121 tggccggcgg cgggcaggtg ctgttctgcg gcaacggcgg cagcgccgcc gacgcgcagc
      181 acctctccgc cgagctgatg gggcggctgc tgctggagcg gtccgcctac cggtcggtgg
      241 cgctgaccac cgacacctcg gccctgacgg cgatcgccaa cgactacagc tacgccgagg
      301 tgttcgcccg ccaggtccag gggctcggcc gggccggcga cgtgctggtg gcgatcacca
      361 ccagcggccg ctccgagaac gtggtccgcg ccgccgccgc cgccaaggac cgcggcctgg
      421 tggtggtcgc cttcctcggt cccgcccgct cgccgctgga ccagaccgcc gacgtcgccc
      481 tgcatgtcga cggcggcacc gccggcctga tccagcaggg ccacatcacc atcggccatg
      541 ccctctgcgg ctgggtggag tcctatctcg cccgccgcca ggcccccgga tctcgtcatt
      601 aggatgcgcc ccgccgtgtt cctcgaccgc gacggcaccc tcgtcgagga ggtctcctac
      661 ctgcacgagc cggacaaggt cgccctggtg gacggcgcgc tgccggcgct gcgccgcctg
      721 gcggacgcca ggttcgcact ggtggtggtc tccaaccagg cgggggtggc gcgcggctac
      781 taccgggagc gggacgtcga ggcggtccac gggcggctgc aggcgctgct ggccgccggc
      841 ggggtcgccc tcgacgggat ctggtactgc ccgcaccacc cggacgggac ggtcgcggga
      901 tacgcgcggg cgtgccgctg ccgcaagccg ggggcgggca tgctggaggc ggcggccgtg
      961 gcgctccgcc tcgacctggc cgcctcctgg ctggtcggca acaaccccag cgacgtcggc
     1021 gccgcccgcg ccgccggggt ggcgccgctg tacgtcacca ctggccgcgc cagcggcgag
     1081 acgcccccgc cgggggtggc ggcgttcccg acgctcgccg ccgccaccgc cgcgatcctc
     1141 agccaacgcg tgggaggctc ggcgggcgag cgggcagcag aattgcggcc aggcccaccg
     1201 tggccgtgac acggcaggcc tgacctggtt gccggctggt cgtgggtcaa accacacgcc
     1261 catggccgac actccgacca gcgtgcctgg ccgtgcctct gtgccccctg aaccccccac
     1321 accggccagc tgctgccgct ggcgtccttc ccatgaagct ggtgcgctcc cctccaccgg
     1381 gcagcttgaa aggatgtccg caagctaacg tttgcactga gcttgcgtcg gtgtcgcaga
     1441 tcacactctg acggtgcttt ccagcaccgc gtgccgggcg acgtcagcgg cgatttagcc
     1501 ggatgctccc ttattgtcag ggtaattcga cgccggcgct cagctcaaca ccagccggcg
     1561 tagcgcctcc gggtcgagca ggcggatcgc gctggtgtcg taggcgatca ggccgcgctt
     1621 gcggaaggag gccagcccgc gggtgacgtt ctcgcggctc gcgccgacca tcccggccag
     1681 gtcacggtgg gtgaggcgca gggcgatcac ctggctgccg tcggcggccg tgtggccgtg
     1741 gcgctcggcc aggccgagca gcgccttggc gaggcggccc ttgacgtcca gggagagcag
     1801 gtcgtcgatc agcgcctccc ggtcggccag gcgcgcggcg accaggcgca gcagcgccgc
     1861 cgacgcgctc ggggtggtgt ccaggaacgc ccagaacgcc tcccggccga tcagccacgc
     1921 cagcgtcggg gaggtggccc gcgccccggc ggtgcgcttg ccgtcgctca gcagcgccag
     1981 ctcgccgatc agctcgcccg ggccgatgat gctgtggacc tgctcggcgc cgccccgggc
     2041 gggggagatg atcgccacct caccggcggt gagcacgtag acgtgcgcgg ccggctcgcc
     2101 ctggcgcatc aggaacgcgc ccgccgggta ggcgaccggc gaggcctgct ggaccagccg
     2161 ctgcagcgcc gcctcctcgc cgccgccgaa cagctcgctc cgccgtagca cctgcagcag
     2221 ctgggcgctg tccggcgccc gccgccgcgg cgtcaccgtg tcgctccaag ctctcggacc
     2281 aggcggcggg cgccgacggc gcgtgccagg gccagcgctt cggcggtgag cggggccgcc
     2341 cggcgggcgt gctggttccc ggcgcggtgc agggcggcga ccaggtccac cagcgtgcgc
     2401 agccgctcca gccggtagcc ggtgggattc agggtggcga gcgcccgctc cagcagcgcg
     2461 accgcctcgg tgtgccgccc ggtcgcggcc gccaggcgtg cctcagtgcg ctgcagccgc
     2521 gcccgcgaca gcacgtcgcc ggcctgcagg cagtgccagg accgcgccac cgccagggca
     2581 gcgccctcca ggtccggctg gtcgccggcc aggcacgcct cggcgtagtg cagctcggcc
     2641 tcagggtcgg cactgccgtc cggcttggcg gtcagggcga tcgcctcggc ggcgaggcgg
     2701 cgggcggcgg ccgcgtcgcc gcgcccagcc gcgacctgga caaggccgag gagcgaccac
     2761 agccgctgca gcgtcagccc gatggtgcag cgctgctggt tggcatgctc atagtggcgc
     2821 cgcgccagct ccaggtcgcc atggagcgcg gcgacctcgc cgagcgcgaa gctcgcgccc
     2881 gcctcggcgt tgcggcgccc gccggcccgc gccgccgccg ccaggctgag cgcctcctcc
     2941 cggatggtgg caagcggggt ggccagctgc agccgcaccg gcagcagcgt cgactgcacc
     3001 tcgatcgcca ggtcaggatg gccgccgtcg cgggcgtggg catgcgcgtc cagcagcagc
     3061 cgctcggtct cgcgcaggtt gccggtgagg tagggctcct gcgccttggc gcgcagcgcc
     3121 ctggccagca gatccgcccg gccggtggtg gtggcgtcgg ccagggcccg gtcggccgcc
     3181 gcggcggagc ggtcgaactc gttgaggcgg ccgtagaggt tgctcagctc cgtatacgcc
     3241 accgcccgcg gcgctggccg ctccgcggct gctgcggcgc gcaggtcggc gatggcggcg
     3301 gcgggatcgc cgatcgcggc gcgcaaggcg ccgcgccgta cccacagctc ggccagctcc
     3361 tccggcggtg tgcccgcgac cccgaccagg gcgagcgcct cgtcgagcat gtgcgccgcc
     3421 gacgccagcg cctcctggtc gcgcagccgc tcggcggcga gccggcaggc acccaccgca
     3481 cgggccgcca gctgctggtc gcccgcggtc ggggtgcgga tctggccggc gagccgcacg
     3541 gccgccacca gatgcggcgc gatcagctcc ggcaggacca gctcatgcgc ctggtggccg
     3601 ctgcgggtca cgatgtggtc ggcgaggcgc cggtgcagct cggaacgggt cgccttgggc
     3661 agcagctcgt aggcgacctc ccggaacaga gtgtgctgga aggtgaacga gttcggccgc
     3721 ccgctgaccc agtccgggac ctgctcgatc agtccgctct ccaacaggac cgtgaggtcg
     3781 gcgtccagcc ggtctcccac cgcgtccccg cgcccgtcgt ggtcgctgcc cgcgccggtg
     3841 cctggcagcg acagcgcggc aagggactcc tgggtgaacc agcggccgat caccgccgcg
     3901 cggcacagga cctgcttgca ggagggcggc aggcggtcga ggcgcgcggc gatcagcgcc
     3961 cgcagggaat cggggatcgg cagtcgcgcc tgggccacct tggcctcgtc ggcctcgcgc
     4021 agcgccaggc cgagctcctt gacgaacagc gggttgccct ggctcgcttc ctggacccgc
     4081 gcgaccaggc cgtcactcag ctccacgtcg ccgccgagca cgccgcgcag cagcgccgcg
     4141 gtgaagcgct ccggcaggca ctccaggtcg atgcgtcggg caccggtgtg ctgcagccac
     4201 gccgggtcca gcggcaggtc cgggcgggtg gtgccgacga aggccagctc gctgtggggc
     4261 gggcagcccc cgctgaccag gatggtggcg acgtcgagca ggtcgcggct ggcccagtgg
     4321 agatcctcca tcacaatgac ggtccgctcg tgggcggcca tgcggcacag caccccggcc
     4381 agcgcggcgg ccagctccct ggcgccgacc tggctcggcg cggtggcgtg ctcggcggcc
     4441 cgttcgcgat ggcgtccgcc acggcgtccg ctggccgact cgagcccgat cagccggcac
     4501 accgccgcgg cggtggtggc ggcctccggg ccggggccgg tccagctctc ggtgacaagc
     4561 gcggtgatcg cctcggccaa ggtgccgctg gggtcagtgg ccgggacgcc cgctgcggcg
     4621 gcgagcacct cgcgcaccgg ccagaacggc aggctctgcc agtccggcag gcagcgtccc
     4681 cacaacacct gcacctggtc gcccaggcgg gccgcggtcg cccgcaccag cgtggtcttg
     4741 ccgaccccgg cctcgccgac cagcagcacc aggcggctga gcacagccgc ctggtcgacc
     4801 gcctggagca gcttcggcag gtcctcctcg cggtcgacca tcaggctcac cggttgtgac
     4861 acggtggtca tgcccgtcac ctcgaccacc tcgaccgggc gctgcttgcc gcgcgccacg
     4921 atccgccggg gcggcccggc caccaccagg tcacgggccg cacgggcggt gcgggtggag
     4981 atcaggatct gacctggctc ggccgcctgc tggatgcgcg cggcggtatg caccgcgtcc
     5041 ccggcgaccc gcaggtcgct ggcccgcagc accgccacca cctcgccggt ggccatgccg
     5101 atcctgagcc ggaggctcgg gccggttccg gccgcctgcg gccgggcggg ttcctccagt
     5161 accgcgcgca tcgccagcgc ggcccgcacc gcccgggcgg cgtcgtcacc gtgggcgacc
     5221 ggaaagccga acaccgccag gatcgcgtcg ccgatgtatt tctccagggt cccgccgcag
     5281 cggcgaatct cggccgcgag gcgatggaag ctctcgtcga gcaggctgcg ggccgcctcc
     5341 gggtccagcg actcgaccag cgtggtggaa ccgaccaggt cggcgaacag cacggtgacc
     5401 acccgccgct cctcgccacc ccgcagtcga ccgccgcagg cggggcagaa ccgggcctcc
     5461 ggcggcgccg ccgcccagca tgcggggcag gcaaccgcat cggcagccgt cccggtcctg
     5521 ttcgaggccg gaccggggag cgtggatgcg ccgcctcgct gtgccagcgg atcagcgggc
     5581 atggttcccg ttggtgctat ttccagggac cgcgttcggg ggccatcgag gagccggccc
     5641 cctcgccctc ctcctcgccg tcaccgtcac cgtcggcggt gtcatcacca ggctcgggtt
     5701 gggcggggtc gtcggggtcc agggtgtcag gctcgctctc ccacgggccg cggagggact
     5761 cggtcccgga cgatcctgga gccgcctggg gatccttgcc ctcgtcaggg tcgatggtgc
     5821 tcacggttgc ctcccgtcac tgtcgtgccc acccgtcatt ggcgtcagtg tcgctgcaag
     5881 ctgtggagcc atctgcaacc ttcgagcatg atcatgccag tccgaccgcg atgccgggtg
     5941 tcagattcaa ttacggtcag gtgtcgaatc ctgacacctt gatcatcctg cccgggtccg
     6001 accccagcgg tggccgccac atggcagcca ctaagatgtg gggattcacc ggaccgctgg
     6061 agagtgacgc ggattcgtat caaggtcgtt tccgacatcc acggcgccgc cgaagcgctc
     6121 cgccgccaag ccgccggcgc cgacgcgctg ctggtctgcg gcgacctcgt caacctgctc
     6181 gactaccgca ccatggacgg gatcgcggtc gagctgtacg gccgcgagac catcgcgcgc
     6241 ttcgtcgaac tgcgcaacgc cgcacggttc gaggaggccg gcggagtcct gcgcaccgcg
     6301 gtcgccggcc gcgagggcga agtgatcgcc cgggtgcgcg ccgacatgcg ggcccagtat
     6361 gaggcggtct tcgccgcctt ccctgagcgg acctacctga ctcacggcaa cgtcgacaac
     6421 gtcgacctgt tccaggacct gctgcgcccg ggcgtgcgcc atctggacgc cgaggcggtc
     6481 gatgtcgacg ggatgcgtgt cggcttcatc ggcggcggcc tgccgcgcgg ccgcaccccg
     6541 cacctgtcgg aggtgaccca cgagcagttc agcgccaagg tggcccgcct gccacgcgtc
     6601 gacgtgctct gcgcgcacat gccgcccgcc atcgacgacc tgcgcttcga cctggccgct
     6661 ggccgcttcg agcctggcag ccaggcgctg ctcgactacg tcgaggaaca ccagcccgac
     6721 tacctgtact acggccatgt ccaccagccc cgcaagagcc gcctgcgcgt cggccgcacc
     6781 cgcttggtga acgtcggcta cttccgcgcc accggccgca tgctggacca tcccgccgac
     6841 tagattcgcc ggaatccgac cgggaatccg accgggaacc ggtgtccgag gagaggagcc
     6901 gtgaccccca tggccgagca cgccgaaggc accatcgagg tctacggcac ccccgccgag
     6961 atcatggggg tgatcgccgg cttcgacgcc tacccggagt gggtcggcaa catggacgag
     7021 gccgaggtcc tggaacgcga ccgccagggc cgcgccacca cggtggcctt caagctccgg
     7081 accccggtga tgaacgccgc ctacaccctc gcctaccgct acgagccgga cgacctcggg
     7141 gtgtcctgga cctacgtgga gggcagcctc caggatctct ccgggcagta cgcgctggag
     7201 ccgctcgacg acggcaccac ccgcgtcacc taccgcctcg aggtcgacct cggcatgccc
     7261 ctgcccggcc tgctcaaacg gcaggcagcc aagcagatcg tgcgctcggc gctgggcgac
     7321 ctgaagcagc gcgtggagtc ggcgtaggct caggcggaga gaccatcacg gcacgaccgc
     7381 tggagcgttc gcttgcgcat cttgttattc actggcaagg ggggcgtcgg caagaccacg
     7441 gtggcggcgg cgaccgccgt gcgcgccgcc gcgctggggc accggaccct ggtcatgtcg
     7501 accgatccgg cgcactcgct ggctgactcg ttcgagttcg agctggggtc caagccccgc
     7561 caggtggctg acaacctgtg ggcggagcag atcgacaccc aggcgcggct ggaggccaac
     7621 tggcgcgaga tccaggacta cgtgatcacg ctgctgaact ggggtggggt ggacgcggtc
     7681 gaggccgagg agctgtcggt gatccccggg ctcgacgagc tgttcagcct catcgacatc
     7741 agtcgctacc agcagcaggg cgcctacgac ctgctggtgg tcgactgcgc gccgacggcg
     7801 gagacgctgc ggctgctgtc gctgccggag gcgctgtcgt ggtacatcga ccggctgttc
     7861 ccgtcgtcgc ggcggatcgc cagtgtggcc cggccgctgc tcgggcggct cggaagcctg
     7921 ccgccgatcg cctccgacgc ggtcttcggc gcggtcgaac ggctctaccg caacctggag
     7981 cgggtccgca tgttgctggc cgagccggag tcgtcgaccg tccggctggt ggtcaacgcg
     8041 gagaagatgg tggtcgccga ggcccggcgg acctacacct acctgtcgct gttcggctac
     8101 cgggtcgacg cggtggtggt gaaccggttg ctgcccgacg acgtcaccga cccgtacttc
     8161 ctgaagtgga aggagatcca ggcggtccat taccgcagca tcaccgagtc gttcgcgccg
     8221 ctgccgatcc tgccctgccg gctgttcgac gacgagatgg tcggcctgga ccggctcggc
     8281 gaggtggccg gggcggtcta cggcgagcgc gacccggccg cggtgctggc gcactcccag
     8341 cccatgcggg tcagcaagac caagggcggc tacgcgctgc tgctcgacct gccgttcgcc
     8401 gagcgtgccg agctggacgt gctgcgcaag ggcgaggaac tgtacgtcaa ggtcggtgcc
     8461 tacaagcgca acgtgctgct gccgcaggcg ctgcagccgc tcgacgtggt ctcggcgacg
     8521 ttgcgcgaca gcaccctgga ggtccgcttc ggggccgctc cgtccagccc gagccggtag
     8581 agtccgctca ggacaccgac gggcaacgcg accaggtcgc cgggagcgtg cggggccggg
     8641 agcatgagga ggacggatga cgcaggacga tgcccctgaa gggttggcac cggagtgccg
     8701 ggtttgcccg ctctgcatgg ggcttgcggt gctccgccag tcccggcccg aggcgctgga
     8761 gcacctgctg aaggcgggcg cggagctgct gctggcggtg cgggcgctgc tcgacggcgc
     8821 cgaagggctg cgcgggtcgg ccactctgca gcgcatcgac atcggctgag gcggtggggt
     8881 ggagatgagc caggacgagg ctgcggccga cacatcgggg ggccccgcgg cagccatggg
     8941 atcggccgtc ggtccttccg gccgcgggac cgtgcccccc gaaccccccg tcccgccgga
     9001 tccggaacgc cggcaggcca tcgggatcga catcggcggc accaagatcg ccgccggggt
     9061 ggtcgccaca ggcggggaga tcatcgaccg gacgctggtc ccgaccccgt cggtggagga
     9121 ccaggagcca accgtggcgg cgatccgcga ggtgatcgac accctgcgga cccgccaccc
     9181 gaccgccgag gcgatcgggg tcggcgccgc cggcctggtc gagtggcccg gtgggcggat
     9241 catctgggcg ccccacaacg cctaccggca gctgccgctg cggcggttgc tctacgaggg
     9301 gaccggcctt ccgacggtgg tcgacaacga cgccaacacc gccgcctggg cggaggcctg
     9361 ctttggcgcg ggcgtgggcg tcgggcacgt cgtgctgctc accgtcggca ccgggatcgg
     9421 tggcgggctg gtgctcggtg ggcagctcta ccgtggcgcg accgggctcg gcgccgaggt
     9481 cggccacatg accgtcgacc cggacggcga cccgtgcgcc tgcggcaacc tcggctgcct
     9541 ggaggcgatg gcatcgggga ccgcgctcgg ccgcgccggg cgcagggcgg ccgccgccga
     9601 cccgggcggc cggctcgccc agctcgccgg cgcgccggac gcggtcaccg gcgaggtcgt
     9661 gttccaggcc gcgcaggagg gcgacccgac cgcccgggag ctgttcgagc agctcggctt
     9721 ctggctcggg gtcggcatcg cctcgctggt cagcctcttc gacccggagc tggtggtgat
     9781 cggcggcggg ctggtggcca ccggcgatct gctgctggag ccggcacggg cggcgatgga
     9841 gcgctacacc ttcgcccgcc cgcaccgcga gctgccgccg gtggtgcccg ccctgctcgg
     9901 ccacgaggct ggcctggtcg gcgccgccgc cctggcgctg ggcacccgtg cctgacgggg
     9961 gcttcggggg gcacagtccc gcggccggaa gcaccgaaag cggtttccat ggcggccgtg
    10021 gggccccccg atgggtcaat cccgcgcgcc ggcttcggat cggtctgacc ctgccgcagt
    10081 tccggcgcga cgccagctcg acgctggcca ccttcgacga gctttggcgg ctcggctacg
    10141 acggcgcctt cgtcttcgac cacctctggc cgctgctgca gccgacccgg ccagcgctgg
    10201 aggggtggac cctgctcgcc acgcttgccg ctcgccgcgg cggcggcgac gggttccgcc
    10261 tcgggacgct ggtgacgcgg gcggggctgc gggcgccgag cctgctcgcg cacatggcgg
    10321 ccaccgtgcg gtcgatcgcg ggtgcaccgc tgatcgccgg ggtgggtgcg ggggatgccg
    10381 ccagccgcgc cgagaatctg gccttcgggc tgccgtacga ggacgcggcc ggccgcctcg
    10441 ccaacgtcga ggcggcggct gtcgccctgg ccgggaccgg ggccgctgag gtgtggatcg
    10501 gcggcctcgg cgcgacgact cgcgcgctcg ccggcagggt ggcggacgcc tggaacgcct
    10561 ggggggtga
//
LOCUS       DASWDC010000158         5163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1289819, whole genome shotgun sequence.
ACCESSION   DASWDC010000158 DASWDC010000000
VERSION     DASWDC010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289819"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VF995_05055"
     CDS             <1..373
                     /locus_tag="VF995_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342966.1"
                     /translation="TIGLVAISEAQRRHAPDLPWEGTVHNGVVVDDFPFRDRAAKEDC
                     GTPVVALRRGAVPEVVADGISGLLCDRPAELPAAIDRAVALNPARCRRHAREHFDVAV
                     MAAGYERVYQRILTRVAAAPA"
     gene            453..1991
                     /locus_tag="VF995_05060"
     CDS             453..1991
                     /locus_tag="VF995_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9342967.1"
                     /translation="MVGGKSADDGAATTYRSTDPSHLAETVAEVRLAPADTFLTACKV
                     AAAAQAAWAEVPAPVRGRAIAAAGRLVETNKQALARLVTREVGKPYAESLGEVQEIVD
                     TCDFFLGEGRRLYGQTVPSEMPDKSLFTFRNPVGVAAIITAGNFPVAVPAWYLVPALL
                     CGNAVVWKPAEYAAASAQALYELFAHAGLPDGVLNLVFADGEATYRGLELALEARVCH
                     KVGFTGSTAVGARIGELCGRHLQSPCLELGGKNPMVIMPDADLDLAAEGALFSGFGTA
                     GQRCTSLGTAIVHQSVHAGFLDRLGGALGAAAIGDPMREVLYGPMLDEKFATRFEGCL
                     DWIGSGQRVLGSAGVGRVSAANPRPGFVGDPEAGLFYHPTVVDGVAPGDRLFQEETFG
                     PIVGVATFSHLSEAIELANAPGYGLSSSIYTRDPKAALTFRRGIGAGMVSVNNSTSGA
                     EAHLPFGGNGKSGNGSRLSGMWVLDQFTRWQAMNWDYSGRLQKAQMDVAEIEPDLSFR
                     LADS"
     gene            complement(2058..3305)
                     /locus_tag="VF995_05065"
     CDS             complement(2058..3305)
                     /locus_tag="VF995_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342968.1"
                     /translation="MADAAVFGFDPDLPSLPQAFDPRTVARLFAPAGGAVGQLRRQDT
                     KYQPGVRCVVTYEVELIGATADHRRRTIGVVTATPQGLERRFMEEDRRLTGLAEALDG
                     RRMAARLAAALGPVEACAATPVRYKPNDRCVIRYELRNADGPRLLFGKLLADGWSAKL
                     ALLDALAVAGHTDEAVPPTLPVVAHLPDLGLLLQPAVDRAVELNRAGFDPAVPAATRA
                     AWLRAAGRHLAGLQGCALPGAPERALAADLDELATYIAPMYQADPPLARRYQAVLDTL
                     EQHVGAAPPPQPVAGHGAFRTDQFLLPGGDAGTADTDTLVMIDLDGACRAEPARDLGN
                     FLAYLDWKTIRRPDLVAVVGEAEGAVLAGYAAQRPLPAAERLDLCRAASLLKIAGRRY
                     RSLATGEWHLVPALVDRAAALLG"
     gene            complement(3308..4603)
                     /locus_tag="VF995_05070"
     CDS             complement(3308..4603)
                     /locus_tag="VF995_05070"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9342969.1"
                     /translation="MTQPRTEGETPRVMLYSQDGFGLGHLRRTSLIAAALTQACSSAA
                     VLTAIDSRLGAFFEPAPNQDYLKLPSIVKIGPGEWQAVGLPLDFPQVHAMRSELLCAT
                     ARTYRPHLLLVDHMPHGAMGELLPTLEALRRDSGTRIVLGLRDIIDAPPVTREVWARE
                     GAYEAIDRFYDQVLVYGRRDVFDLGAQYGLPPSVASRVRYTGYVASPSKGLEAARLRT
                     RYAAGEGSGRALIVAMAGGGADGYPLLRAVLDALPLVRERQPCTAVLVAGPFMPSGRR
                     RELQRRARAADARVHVSVSDTLSHIRAADVVAAMAGYSTTVEVLCARTPAILVPRRGP
                     SAEQRTRAGLFAGRGWIRAIDPDDLDVETLAAALLASLAQGTVRGRPACDGLRVAVGS
                     LLGLLGGDCAEAAAAAGMLEEGAAAPAPPANLLAPLKLE"
     gene            complement(4723..>5163)
                     /locus_tag="VF995_05075"
     CDS             complement(4723..>5163)
                     /locus_tag="VF995_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9342970.1"
                     /translation="VPAPVPVAEPPPAARPGPRPRPRPRPSRWWLAVVALLFGFALAG
                     PVVIVQRQLALHRPGGGGAGGPGGAVTMAGLAFKPGTVVVKRGTTVVFHNDDQAPHTV
                     TADSGAFDSGVLEPGATFSITVSSGLTYHCTIHAFMKARIYVAS"
BASE COUNT          675 a         1823 c         1984 g          681 t
ORIGIN      
        1 caccatcggc ttggtcgcca tctccgaggc gcaacgccgc catgcccccg acctgccctg
       61 ggaggggacg gtgcacaacg gcgtggtggt ggacgacttc cccttccggg accgtgccgc
      121 caaggaggac tgcggcaccc cggtggtggc gctgcgccgg ggcgcggtgc ccgaggtcgt
      181 cgccgacggg atcagcggcc tgctgtgcga ccggcccgcc gagctgccgg cggcgatcga
      241 ccgggccgtc gcgctcaacc cggcccgctg ccgccgtcac gcccgcgagc acttcgacgt
      301 cgccgtgatg gccgccggct acgagcgcgt ctaccagcgc atcctcacga gagtcgcggc
      361 ggcgccagcg tagactggct ggcggggacg accccagccc tgcccggtcg cgcagcgagg
      421 tgcacatgcc agacgtcctg aaactcgtct ccatggttgg cggaaaaagt gccgacgacg
      481 gcgccgcgac cacctaccgg tcgactgacc cgagccatct ggccgagacc gtcgccgagg
      541 tgcgcctcgc accggccgac accttcctga ccgcctgcaa ggtcgccgcc gccgcccagg
      601 ccgcctgggc ggaggttcca gcgccggtgc ggggccgggc gatcgccgcg gccggacgcc
      661 tggtcgagac gaacaagcag gcgctcgctc gcctggtgac ccgtgaggtc ggcaagccct
      721 atgccgagtc gctcggcgag gtccaggaga tcgtcgatac ctgcgacttc tttctcggcg
      781 aggggcggcg cctgtacggc cagacggtgc cgtcggagat gccggacaag agcctgttca
      841 ccttccgcaa cccggtcggc gtggccgcga tcatcacggc cgggaacttc ccggtggcgg
      901 tgccggcgtg gtacctggtg ccggcgctgc tgtgcggcaa cgcggtggtg tggaagccgg
      961 cggagtacgc cgccgccagc gcgcaggcgc tctacgagct gttcgcccac gccgggctgc
     1021 ctgacggcgt cctcaatctg gtcttcgccg acggcgaggc gacctaccgg gggctggagc
     1081 tggcgctgga ggcgcgggtc tgccacaagg tcggcttcac cggctcgacg gcggtcgggg
     1141 cccggatcgg cgagctgtgc gggcgccatc tgcagtcgcc ctgtctggag ctgggcggca
     1201 agaacccgat ggtgatcatg ccggacgccg acctcgacct tgccgccgag ggcgcgctgt
     1261 tctccggctt cggcaccgcc gggcagcgct gcacctcgct cggcaccgcg atcgtgcacc
     1321 agtcggtgca cgccggcttc cttgaccggc tcggcggcgc gctgggcgcc gccgcgatcg
     1381 gcgacccgat gcgcgaggtg ctctacgggc cgatgctcga tgagaagttc gccacccgct
     1441 tcgagggctg tctcgactgg atcggctcgg gccagcgcgt gctcggctcg gccggggtcg
     1501 ggcgcgtcag cgccgccaac ccgcgcccgg gcttcgtcgg cgaccccgag gccgggctgt
     1561 tctatcaccc gacggtggtg gacggcgtcg cccccggcga ccggctgttc caggaggaga
     1621 ccttcgggcc gatcgtgggc gtggccacct tcagccacct cagcgaggcg atcgagctgg
     1681 ccaacgcgcc cgggtacggc ctgtcgtcgt cgatctacac gcgcgacccg aaggcggcgc
     1741 tcaccttccg gcgcggcatc ggcgccggca tggtgagcgt caacaactcg acctccggcg
     1801 cagaggcgca cctgccgttc ggcggcaacg gcaaatcggg caacggcagc cgcctgtccg
     1861 gcatgtgggt gctcgaccag ttcacccgct ggcaggcgat gaactgggac tactccggcc
     1921 gcctccagaa ggcccagatg gacgtggccg agatcgagcc tgacctgtcg ttccgcctgg
     1981 ctgactcctg atcctgatcc tgtgtgccgc atcctgatcc tgtgtgccgc ctgctggaca
     2041 cggatccggc tggcggttca gccgagcagg gcggcggcgc ggtcgaccag ggcggggacc
     2101 aagtgccatt cgccggtggc gaggctgcgg tagcgccgcc cggcgatctt gagcagagac
     2161 gcggcgcggc agaggtcgag ccgttccgct gctggcaggg gacgctgcgc ggcgtagcca
     2221 gcgaggacgg cgccctcggc ctcgccgacg acggcgacga ggtccgggcg ccggatcgtc
     2281 ttccagtcga ggtaggcgag gaagttgccg aggtcgcgcg ctggctcggc gcggcaggcg
     2341 ccgtccaggt cgatcatcac cagcgtgtcg gtgtccgccg tgccggcgtc gccaccgggc
     2401 agcaggaact ggtcggtgcg gaaggcgccg tggccggcga cgggctgcgg gggtggcgcg
     2461 gcgccaacat gctgctccag cgtgtcgagg accgcctggt agcgtcgtgc cagcggcggg
     2521 tcggcctggt acatgggcgc gatgtaggtg gccagctcgt cgaggtcggc ggcgagcgcc
     2581 cgctccggcg cgcccggcag cgcgcagccc tgcaggccgg caaggtggcg gccggcggca
     2641 cgcagccagg cggcacgcgt cgcggccggg acggccgggt cgaagccggc gcggttcagc
     2701 tccaccgccc ggtccaccgc cggttgcagc aacaggccga ggtcggggag gtgcgccacc
     2761 accggcaggg tcggcggcac cgcctcgtcg gtgtgcccgg ccaccgccaa ggcgtcgagc
     2821 agcgccagct tggcgctcca gccgtcagcg agcagcttgc cgaacagcag ccgcgggccg
     2881 tcggcgttgc gcagctcgta gcggatcacg cagcggtcgt tgggcttgta gcgcacgggg
     2941 gtagcggcgc aggcctcgac cgggccgagc gccgccgcga gccgggcggc catgcgccgg
     3001 ccgtcgagcg cctcggcgag accggtgagg cggcggtcct cctccatgaa gcggcgctcg
     3061 aggccctgcg gggtggcggt gacgacgccg atggtgcggc ggcggtggtc cgcggtggca
     3121 ccgatcagct cgacctcgta ggtgaccacg cagcgcacgc ccggctggta tttggtgtcc
     3181 tggcggcgga gctgccccac cgcgccaccg gcgggcgcga acaggcgcgc gaccgtcctc
     3241 gggtcgaagg cctgcggcag gctcggcagg tctgggtcga agccgaacac ggcggcgtcg
     3301 gccaccccta ctccagcttc agcggcgcga gcaggttggc gggcggcgcc ggggcggcgg
     3361 cgccctcctc cagcatcccg gcggcagccg cggcctcggc gcagtcgccg ccgagcagcc
     3421 ccagcagact gccgaccgcg accctgagcc cgtcgcaggc gggccggccc cggaccgttc
     3481 cctgggcgag gcttgccagc agcgcggcgg ccaaggtctc gacgtcgagg tcgtcggggt
     3541 cgatggcgcg gatccagccg cggcccgcga acaggccggc ccgggtgcgc tgctcggcgc
     3601 tggggccccg ccgcgggacc agaatcgcgg gggtgcgggc gcacagcacc tcgacagtgg
     3661 tcgagtagcc ggccatggcg gcgaccacgt cggcggcgcg gatgtggctc agcgtgtcgc
     3721 tcaccgagac gtgcacgcgg gcgtcagcgg cgcgggcgcg gcgctgcagc tcgcgccgcc
     3781 ggccgctggg catgaacggc ccggcgacga gcaccgcggt gcagggctgc cgctcccgca
     3841 ccagcggcag ggcgtccagc acggcgcgca gcagcgggta gccgtcggcg ccgccgcccg
     3901 ccatcgccac gatcagggca cggccgctgc cctcgccggc ggcgtacctg gtccgcagcc
     3961 gcgcggcctc cagccctttg cttggcgagg cgacgtagcc ggtgtagcgc acccggctgg
     4021 ccaccgacgg cggcaggccg tactgcgcgc cgaggtcgaa gacgtcgcgc cgcccataga
     4081 ccaggacctg gtcgtagaag cggtcgatcg cctcgtaggc gccctcgcgg gcccacacct
     4141 cgcgggtgac cggcggcgcg tcgatgatgt cgcgcaggcc cagcacgatt ctggtgccgc
     4201 tgtcgcggcg cagggcctcc agggtgggca gcagctcccc catggcgccg tgcggcatgt
     4261 ggtcgaccag cagcaggtgc ggccggtagg tgcgggccgt ggcgcacagc agctcgctgc
     4321 gcatcgcgtg gacctgcggg aagtcgagcg ggagcccgac cgcctgccat tcgcccggcc
     4381 cgatcttcac gatcgagggg agcttgaggt agtcctggtt gggtgccggc tcgaagaagg
     4441 cgccaagccg ggagtcaatg gcggtcagta ccgccgcgct gctgcacgcc tgggtcaggg
     4501 cggccgcgat caggctggtg cggcgcagat ggccgagccc gaagccgtcc tgggaataca
     4561 gcatcacgcg cggggtctca ccctcggtgc gcggctgggt cacacgtccc cctctccatc
     4621 ctgtttcgac aggtggcgga gctgtcaggg ccgaccggca cggccggcct ggagctggat
     4681 acggtctcgc cgctcgatcc ggttcattag gtaggtagag cctcaggagg cgacgtagat
     4741 acgtgccttc atgaacgcgt ggatggtgca gtggtaggtg aggccgctgc tgacggtgat
     4801 gctgaaggtg gcgccgggtt ccagcacgcc cgaatcgaag gcgccggagt cggcggtgac
     4861 cgtgtggggc gcctggtcgt cgttgtggaa caccacggtc gttccccgtt tgacgaccac
     4921 ggtgcccggc ttgaaggcga gtccggccat ggtgacggcg ccgcccgggc cgccagcacc
     4981 gccgccaccg gggcggtgca gggcgagctg gcgctgcacg atcaccaccg gacccgcgag
     5041 cgcgaacccg aacagcagcg cgaccaccgc caaccaccag cggctggggc gggggcgggg
     5101 gcgggggcgg gggccggggc gcgcggctgg tggcggttcc gcgaccggca cgggagcggg
     5161 aac
//
LOCUS       DASWDC010000159         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1455510, whole genome shotgun sequence.
ACCESSION   DASWDC010000159 DASWDC010000000
VERSION     DASWDC010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455510"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VF995_05080"
     CDS             <1..809
                     /locus_tag="VF995_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX9342971.1"
                     /translation="ARGVLSVASQPLVMADRRGLLERLLVALSRPGVDGVLATADVID
                     DLLLLGALDGKLAIGSMNRGGLSGAVFELDDRFTGYSVAGLQAAGLDGGKMLLRLQDD
                     DPGTSETLEGCAMAVEGLAAAGLMTMVEPLAARRGEDGRVHLDPDLERMTRAISIASA
                     LGSSSARTWLKVPAVPDMARVMAASTLPALVLGGDPSAGADQVRASWREALALPTVRG
                     VAAGRALLYPTDGDVAAAVDAAVGLLAEAKAGAETDARVKADGQAGAKGA"
     gene            931..>1538
                     /locus_tag="VF995_05085"
     CDS             931..>1538
                     /locus_tag="VF995_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342972.1"
                     /translation="MLRDRRSRLLAATVPLIALAILAIVASSGFSTRGPAAPSAKAAN
                     ARIIPLGHNNLGGAQGGGKGGEGIALLVSGGRRYLFTAAESGPNCFSVVDVTDPGRLV
                     LVRQIDVPAAVVRCNSLDLSGHTLAVAYQTTLVGQQPAGLQLWDVNDPANPVKAGFLD
                     TSGPHSRGVHHVWFVDGHFAYIATGAADFTPTNPNDDQFLMIV"
BASE COUNT          209 a          562 c          549 g          218 t
ORIGIN      
        1 cggcgcgcgg ggtgttgtcg gtggccagcc agccactggt catggccgac cggcgcgggc
       61 tgctggaacg gctgctggtg gcgctgtcgc ggcccggcgt cgacggcgtg ctcgcgaccg
      121 ccgacgtgat cgacgacctg ctgctgctcg gggcgctcga cggcaagctc gcgatcggct
      181 cgatgaaccg gggcggcctc tccggcgccg tcttcgaact ggacgaccgc ttcaccggct
      241 actcggtggc agggctgcag gcggcggggc tggacggcgg caagatgctg ctgcggctcc
      301 aggacgacga cccggggacc agcgagaccc tggagggctg cgcgatggcg gtggaggggc
      361 tggccgccgc cggcctgatg acgatggtcg agccgctcgc cgcccggcga ggcgaggacg
      421 gcagggtgca cctcgacccg gacctggagc ggatgacgcg ggcgatctcg atcgcgtccg
      481 ccctcgggtc gagctcggcg cgtacctggc tcaaggtgcc ggcggtcccc gacatggccc
      541 gggtgatggc cgcctcgacc ctgccggcgc tggtgctcgg cggcgacccc agcgctggcg
      601 ccgaccaggt gcgggcgtcg tggcgcgagg cgctggcgct gccgaccgtc cggggtgtcg
      661 cggcgggccg cgccctgctc taccccaccg acggcgacgt cgcggcggcg gtcgacgcgg
      721 cggtcggcct gctcgccgaa gccaaggccg gcgccgagac ggacgccagg gtcaaggcgg
      781 acggccaggc cggcgccaag ggcgcgtagc cgcggacgcg gttccccgag ccgcttgccc
      841 cgagttgccg cgaatgcgtt gacagacccc tcttgaatca acgaaattat ataatcgctt
      901 atatagaatc gtttggttga gaggtgcgcc atgctgaggg atcgccgctc acgcctgctc
      961 gccgcaaccg tcccgctgat cgccctggcc atcctggcga tcgtcgccag ctccggcttc
     1021 tcgacgagag gtccggcggc gcccagcgcc aaagccgcca acgcccggat catccccctc
     1081 ggccacaaca acctcggcgg tgcccagggt ggcggcaaag gtggcgaggg catcgccctg
     1141 ctggtgtccg gcggccgccg ctacctgttc acagcggccg agtcgggacc gaactgcttc
     1201 agcgtggtcg acgtcaccga ccccggcagg ctggtcctcg tgcgccagat cgacgtgccg
     1261 gccgcggtgg tgcgctgcaa ctcgctcgat ctctccggcc acaccctcgc cgttgcctac
     1321 cagacgaccc tggtgggcca gcagccggcc ggcctgcagc tctgggacgt caacgacccc
     1381 gccaacccag tgaaggccgg gttcctggac acctccgggc cgcactcccg cggggtccac
     1441 cacgtctggt tcgtcgacgg ccacttcgcc tacatcgcca ccggggccgc cgacttcacc
     1501 cccaccaacc ccaacgacga ccagttcctg atgatcgt
//
LOCUS       DASWDC010000160         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_288497, whole genome shotgun sequence.
ACCESSION   DASWDC010000160 DASWDC010000000
VERSION     DASWDC010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288497"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VF995_05090"
     CDS             <1..1194
                     /locus_tag="VF995_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9342973.1"
                     /translation="PPSRLRLADLPATATVGLRARRLRAALSALGIAIGIAAIVGVLG
                     ITRSSQSDLLAQLDQLGTNLLTVVNGHSVNGEEVELPASASRMIGRVDGVEHVAPTAE
                     LSSQNVFRSDKIPAFQTSGLAVRACDASLLGTLDAHLHDGTFLTRASARFPAVVLGHD
                     AATALGIDHVDLAHPTRIWIGRHWFAVVGILDPIQFAPEVDSSALVGFPVAQRLLGYD
                     GHPSRIYVRTVTDQTSAVAALLAPTASPDQPSDVDVSRPSDALTARLAVASASTTLFL
                     GLGAVALLVGGIGIANVMVIGVLERRGEIGLRRALGATRGHVAAQFLVESLLLSALGG
                     ALGVLLGSGLTIGLATQRHWTVLIPPATVAAGLGTALAIGAVAGLYPASRAARLSPTD
                     ALRTV"
     gene            complement(1204..2235)
                     /gene="rlmB"
                     /locus_tag="VF995_05095"
     CDS             complement(1204..2235)
                     /gene="rlmB"
                     /locus_tag="VF995_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX9342974.1"
                     /translation="MAPRQLGRPGAGGGDRDGAAASRSGARSSSRSGVEPPRRPQAPA
                     EKRRAAGKAKAKSGAATAKADSGSGGAQAAKQARRAAMVEAARAALRPGPGQDLIEGR
                     RPVLEALRSGRPVDRVLVAAGAGEHGPLADLLALARERGVRVESVPRSMLDAAASTGA
                     NQGVLAVVAAIEPIGIDELLALPLSTPEPPFFLALDGVEDPRNLGALVRSAGAAGCHG
                     VVVPRHRSAPLSAVAVKASAGALQHVPVAEAVNLARALEHLREAGLWCIGLDAESDAS
                     LFEIDLADEPVCVVVGGEGAGLHRLVREACDVLVRIPMAGHVESLNASVAGALALFEV
                     RRRRAGKLT"
     gene            complement(2217..>2619)
                     /locus_tag="VF995_05100"
     CDS             complement(2217..>2619)
                     /locus_tag="VF995_05100"
                     /inference="COORDINATES: protein motif:HMM:NF020752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEX9342975.1"
                     /translation="AGGPGAGGWAGRFNAALDDDLNVPAALAVLFELVADANPLIAKA
                     ERGDAGAVAALRGRLGTFTGLAARLGFTPLLDLPDASAFPAVLDLVLELRDQARAARD
                     FATSDLIRERLASAGIRVEDRPGGSRWHLAN"
BASE COUNT          311 a         1040 c          938 g          330 t
ORIGIN      
        1 ccgccgtccc ggctgcgcct cgccgacctg cccgcgaccg cgaccgtcgg gctgcgcgcc
       61 agacggttgc gggccgccct gtcggcgctc gggatcgcca tcggcatcgc cgccatcgtc
      121 ggcgtgctcg gcatcactcg ctccagccag tccgacctgc tggcccagct cgaccagctc
      181 ggcaccaacc tgctcaccgt ggtcaacggc cacagcgtca acggtgaaga ggtcgagctg
      241 ccagcgagcg cgagcaggat gatcggccgg gtcgacgggg tcgagcacgt cgccccgacc
      301 gcggagctca gcagccagaa cgtcttccgc agcgacaaga tccccgcgtt ccagaccagc
      361 ggactggccg tgcgggcctg cgacgcctcg ctgctcggca cactcgacgc ccacctccac
      421 gacggcacct tcctcacccg ggccagcgcc cgcttcccgg ccgtcgtgct cggccatgac
      481 gccgccaccg ccctcggcat cgaccacgtc gacctcgccc atccgacccg catctggatc
      541 gggcgccact ggttcgccgt ggtcgggatc ctcgacccga tccagttcgc ccccgaggtc
      601 gacagctccg ccctggtcgg cttcccggtg gcgcagcgcc tgctcggcta cgacggccat
      661 cccagccgca tctacgtgcg cacggtcacc gaccagacca gcgctgttgc cgcgctgctc
      721 gcgccgaccg ccagccctga ccaacccagc gacgtcgacg tcagccgtcc ctccgacgcg
      781 ctcaccgccc gcctcgcggt cgccagcgcc tccaccacgc tgttcctcgg cctcggcgcg
      841 gtggcgctgc tggtcggcgg gatcggcatc gccaacgtga tggtgatcgg cgtgctggaa
      901 cggcgcggcg agatcggcct gcgccgcgcc ctcggcgcga cccgcgggca cgtcgccgcg
      961 cagttcctgg ttgagtcgct gctgctgtcg gcgcttggcg gcgcgctcgg cgtgctgctc
     1021 ggcagcggcc tcaccatcgg cctggccacc cagcgacact ggacggtgtt gatcccaccg
     1081 gccacggtcg ccgccgggct cggcacggcg ctggcgatcg gtgcggtcgc cggcctctac
     1141 ccagcgtcgc gggcggcccg gctctccccc accgacgcgc tgcggacggt atagctggtg
     1201 gcgctatgtc agcttgccgg cacggcggcg acgcacctcg aacagggcga gggccccggc
     1261 gacggaggcg ttgaggctct ccacgtgccc ggccatgggg atgcggacca gcacgtcgca
     1321 ggcttcgcgg acgaggcggt gcaggcccgc gccctcgccg ccgaccacga cgcagacggg
     1381 ctcgtcggcc aggtcgatct cgaacaggct ggcgtcggac tcggcgtcca ggccgatgca
     1441 ccacaggccg gcctcgcgca ggtgttccag cgcgcgggcg aggttgacgg cctcggcgac
     1501 cgggacgtgc tgcagggcgc cggcggaggc cttgaccgcg accgccgaca gcggcgcgct
     1561 gcggtgccgg gggaccacca cgccgtgaca gccggcagcg ccggcgctgc gcaccagcgc
     1621 gccgaggttg cgggggtcct ccacgccatc gagcgccagg aagaagggcg gctccggcgt
     1681 cgagagcggc agcgccagca gctcgtcgat gccgatcggc tcgatcgcgg cgactaccgc
     1741 gaggacgccc tggttggcgc cggtgctcgc ggcggcgtcg agcatcgagc gcgggacact
     1801 ctcgacccgc accccccgct cccgcgccag tgccagcagg tcggcgaggg ggccgtgctc
     1861 ccccgcgccg gctgccacga gcacccggtc gaccggccgg ccagaacgca gcgcctccag
     1921 caccggccgc cggccctcga tcaggtcctg accgggtccg ggccgcaacg ccgccctggc
     1981 cgcctcgacc atcgcggccc gccgtgcctg cttggccgcc tgcgccccgc cgctgccgct
     2041 gtcggctttg gccgtggcgg cgccggactt cgccttggcc ttgccggcgg cgcggcgctt
     2101 ctcagccggc gcctgcggcc gccgcggcgg ttcgacgccg gacctggagc tggagcgcgc
     2161 gccggacctg gaggcggcgg ccccgtcgcg gtcgccgcct ccagcacccg gccgtcctag
     2221 ttggcgaggt gccatcggga gcctcccggg cggtcctcga cccggatgcc ggcgctggcg
     2281 agccgctcgc ggatgaggtc ggaggtggcg aagtcccggg cggcgcgggc ctggtcgcgc
     2341 agctccagca ccaggtcgag cacggccggg aaggcgctgg cgtcgggcag gtcgagcagc
     2401 ggcgtgaagc cgaggcgtgc cgccagcccg gtgaaggttc ccagccgccc gcgcagcgcc
     2461 gcgactgcgc cggcgtcgcc gcgctcagcc ttggcgatca gcgggttggc gtcggcgacc
     2521 agctcgaaca ggaccgccag cgcggctggc acgttgaggt cgtcgtcgag tgcggcgttg
     2581 aagcgcccgg cccagccccc cgcccccggg ccgcccgcg
//
LOCUS       DASWDC010000161         4526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_539248, whole genome shotgun sequence.
ACCESSION   DASWDC010000161 DASWDC010000000
VERSION     DASWDC010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539248"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VF995_05105"
     CDS             complement(<1..912)
                     /locus_tag="VF995_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HEX9342976.1"
                     /translation="MAAVVAVPAIVLRLLGVQLADELALLVFGAAVVAAAFLLAWAAE
                     ASEVDISGSLAIALLALIAVLPEFAVDLYFAFTAGSRKSYAAYAAANMTGSNRLLIGF
                     GWSLVALVYIIAARLRAGRGPQRRSAGPKAPATCVRLWPENRIELGFLGLASLYAVVI
                     PLTHKLAWYDAVVLIGLFGLYMWRTGQAEHEEPDLVGVSARLAALPRARRRAAVIALF
                     CVAALVVLAAAKPFAEGLIGTGRAFGIDEFLLVQWLAPLASEAPELIVATVFALRGNG
                     AKGVGTLLAAEVNQWTLLVGALPLAYLA"
     gene            1171..2346
                     /locus_tag="VF995_05110"
     CDS             1171..2346
                     /locus_tag="VF995_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9342977.1"
                     /translation="MSVIQIPDELLALREAIRTFVQREIAPKEALYAQEITETGTFSA
                     LEQEKKALRKRSAAEGYYTLFLPESVGGGGVGYLGMVLCVEETMRHGLLLAMPGGILP
                     SVEGPTHILLDGTKEQQERYLFPTMRAEREACFALTEPDAGSDATRIRTRAERVDGGW
                     VINGRKHFITHGAEADYAILFAVTTPGLGAKGGISCFLVDKDTPGFEVGRRQRTMYDN
                     NQAELVFTDCRVGEDALLGREGYGFYSAMRWINGGRVSIAAMAIGVAQYLYERMLEYA
                     KVREAFGRKIGANQYVQGMIVDSLAELEQGRLLVYRQADAIDSGAGGRTEAALAKYVA
                     TEMVGRVADRAIQVFGGNGYMTEMGIERYARFVRAMRLYEGTSEILKTNIARGIGLA"
     gene            2419..2634
                     /locus_tag="VF995_05115"
     CDS             2419..2634
                     /locus_tag="VF995_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342978.1"
                     /translation="MRANVVLAAAIWTIYVWVTRTWNIISDPDPAHGFAFKAVHVLLA
                     LISIAFAVALGVIGLQLRREAGGRATR"
     gene            2713..3372
                     /locus_tag="VF995_05120"
     CDS             2713..3372
                     /locus_tag="VF995_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342979.1"
                     /translation="MFVQILQGKVADAGGVRSELDRWWRELGPAAPGWRRLTAGLSDD
                     GELLALLRFDSRESYRRYRARPEQKAWLGGLEFCLAAPVTIIECSAGYLLEAGDSEAA
                     GFIQVVQGRTTDPARFAATRPEAEWTLRRHAPHVLGVVFLEHGGSDGYFTEVTYCTSE
                     RETRAAERQMPVEMAVLLGTRRSFVEDFRLIECRELGICGPSASAIQRSAAATAAPIP
                     A"
     gene            complement(3420..>4526)
                     /gene="larC"
                     /locus_tag="VF995_05125"
     CDS             complement(3420..>4526)
                     /gene="larC"
                     /locus_tag="VF995_05125"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX9342980.1"
                     /translation="RVVVRAPGPSVVRDWAALRAVLEGADLPEPVRARALSTFTRLAE
                     AEGRVHRMPPERVQFHEVGAVDAVADVVGAALLLDDLGVTEVWASPVAVGTGRTPGGG
                     HGSESAGSPDASSVLPVPPPAVVELLRGVPIYGGGVEVELTTPTGAAILAANAARFAA
                     LPPLKVAAVGYGAGGRRLRELPNLLRVLLGERIQVEGAAAPGAGGDGALGGSALVLEA
                     NIDDMTPELTPWVLERLLEAGAADAWITPVHMKKGRPGITLSVLCVPGQEAALRQVVW
                     RETSTLGMRGLPVRKWMLERRTVTVELPGPSADGAPVRVRVKLGLDGDRVVNVAPEYA
                     DCARLASLTGQPLKEVMARAQAAALALLDSDQPA"
BASE COUNT          710 a         1641 c         1590 g          585 t
ORIGIN      
        1 ggcgaggtag gcgagcggca gggcgccgac caggagggtc cactggttca cctcagcggc
       61 gagcagcgtg ccgacgccct tggccccgtt gccacgcagc gcgaacacgg tcgccacgat
      121 cagctcaggc gcctcggacg ccagcggcgc gagccactgg accagcagga actcgtcgat
      181 gccgaaggcg cgaccggtgc cgatcaggcc ctccgcgaac ggcttggctg ccgcgagcac
      241 caccagcgcg gcaacgcaga acagcgcgat caccgccgcc cggcgccgcg cccgcggcag
      301 cgccgccagc ctggccgaca cgccgaccag gtcgggctcc tcatgctccg cctggccggt
      361 gcgccacatg tagagcccga acagcccgat cagcaccacc gcgtcgtacc aggcgagctt
      421 gtgggtgagg gggatcacca ccgcgtacag cgacgccagc ccgaggaagc ccagctcgat
      481 ccggttctcg ggccagaggc gcacgcaggt cgccggcgcc ttcgggccgg cggagcggcg
      541 ctgcggtccg cgcccggcgc ggagcctggc ggcgatgatg tagacgagtg cgaccagcga
      601 ccagccgaag ccgatcagca gccggttgct gccggtcatg ttggcggcgg cgtaggccgc
      661 gtagctcttc cgggacccgg cggtgaaggc gaagtacagg tcgacggcga actcgggcag
      721 caccgcgatc agcgccagca gagcgatcgc caggctgccg gagatgtcga cctccgacgc
      781 ctcggccgcc caggccagca ggaacgccgc cgcgaccacc gccgccccga agaccagcag
      841 ggcgagctcg tcggcgagct ggacgccgag caaccggagc acgatggcgg ggacggccac
      901 gactgccgcc accccgatct tccctcgcca aggcaccgcc caacgctatc ccgccggaca
      961 agtgatcgcc agcacccgcc tcgcggcttc ccgaaggctg tggataaccc cgacgccccc
     1021 caggccaaat cggatagcgt tgaaacaggg ggacccccct cgcgcgggtt ttggcgtgcg
     1081 cgctgagccg cgggctctgt ggacaacctc tgtcgcaccc gctccattct ggttaccctg
     1141 gggtaaacca ggggtgcaaa ggagtgccag atgagcgtca tccagatccc cgatgaactg
     1201 ctcgcgctgc gggaggcgat ccgcaccttc gtccagcggg agatcgcccc caaagaagcc
     1261 ttgtacgcgc aggagatcac cgaaaccggc accttcagcg ccctcgaaca ggagaagaag
     1321 gcgctgcgca agcgctcggc ggcagagggg tactacaccc tgttcctgcc ggagtctgtc
     1381 ggcggcgggg gagtcggcta cctcgggatg gtgctctgcg tcgaggagac gatgcgccac
     1441 ggcctgctgc tcgccatgcc cggcgggatc ctgccgagtg tggagggtcc cacccatatc
     1501 ctcctggacg gcaccaagga gcagcaggag cgctacctgt tcccgaccat gcgggccgag
     1561 cgcgaggcgt gcttcgcgct caccgagccg gacgccggct cggacgcgac ccggatccgc
     1621 acccgcgccg agcgcgtcga cggcggctgg gtgatcaacg ggcgcaagca cttcatcacc
     1681 cacggtgccg aggcggacta cgcgatcctg ttcgccgtca ccacccccgg cctgggtgcc
     1741 aagggcggca tctcctgctt cctggtcgac aaggacaccc ccggcttcga ggtgggccgc
     1801 cgccagcgga ccatgtacga caacaaccag gccgagctgg tcttcaccga ttgcagggtc
     1861 ggcgaggacg ccctgctcgg ccgcgagggc tacgggttct actcggcgat gcgctggatc
     1921 aacggcggcc gggtctcgat cgcggcgatg gccatcggcg tcgcccagta cctgtacgag
     1981 cgcatgctcg agtacgccaa ggtccgtgag gcgttcggcc gcaagatcgg cgccaaccag
     2041 tacgtccagg ggatgatcgt ggactccctc gccgagctgg agcagggccg cctgctggtc
     2101 taccgccagg ctgacgcgat cgacagcggc gccggcggcc gcaccgaggc cgccctcgcc
     2161 aagtacgtcg ccaccgagat ggtcggccgc gtcgccgacc gcgcaatcca ggtgttcggc
     2221 ggcaacgggt atatgacgga aatggggatc gagcgatacg cgcgcttcgt ccgtgctatg
     2281 cgcctttatg agggcaccag cgagatcctc aagaccaaca tcgctcgcgg gatcgggtta
     2341 gcatgacgca atgagcggca gggggagcgc tgcaaggcgg cgcacgatgc gggtggtgcg
     2401 ctgatgcgat ccgcacggat gcgagccaac gtggtgctgg ccgccgcgat ttggacaatc
     2461 tatgtgtggg tcaccagaac ctggaacatc atcagcgacc ccgacccggc gcacggtttc
     2521 gccttcaagg ccgtccacgt gctgctcgcg ctgatctcga tcgcgttcgc ggtcgcgctg
     2581 ggcgtcatcg ggttgcagtt gcgccgtgag gcgggtgggc gcgcgaccag ataacgcaca
     2641 ggcagcgcag cggtcgcatg cagtcatcgt cgagaatcac caggcagaca ggcgtggagg
     2701 ggggtgtctg gcgtgttcgt gcagatcctg cagggaaagg tggcggacgc tggtggggtg
     2761 cgcagcgagc tggaccgctg gtggcgggag cttggtccgg ccgcgccggg ttggcggcgg
     2821 ctcaccgccg ggctgagcga cgacggggag ctgctggcgc tgctccgctt cgactcgcgc
     2881 gagtcgtacc gccgctaccg cgcccgcccc gagcagaagg cctggctggg cggcctcgag
     2941 ttctgcctgg ccgcgccggt cacgatcatc gagtgctcgg cggggtatct gctggaggcc
     3001 ggcgacagcg aggcggccgg gttcatccag gtcgtccagg gccgcacgac cgacccggcg
     3061 cgcttcgccg ccacccgccc ggaggcggag tggaccctgc gccgccacgc cccgcacgtc
     3121 ctcggggtgg tgttcctgga gcacggcggc agcgacggct atttcaccga ggtcacctac
     3181 tgcaccagcg agcgcgagac gcgcgcggcc gagcgccaga tgccggtcga gatggccgtg
     3241 ctgctcggca cccggcgcag cttcgtcgag gacttccgcc tgatcgagtg ccgcgagctg
     3301 ggcatctgcg gcccaagcgc gagcgcgatc cagcggagcg cggctgccac ggccgccccc
     3361 atccctgcct aggcacaggt gcggagggga gcggcccggc gggccgctca ggcgagggct
     3421 caggctggct gatcgctgtc cagcagcgca agtgcggcag cctgggcccg tgccatgacc
     3481 tccttgagcg gctggccggt caaggaggcg agccgggcgc agtcggcgta ctccggagcg
     3541 acgttgacga ctcggtcgcc gtccagcccg agcttgaccc ggacccgcac tggggccccg
     3601 tcggcgctgg gaccaggcag ttcgaccgtg accgtgcgcc gctccagcat ccacttgcgc
     3661 actggcaggc cccgcatccc cagggtcgag gtctcgcgcc agaccacctg gcgaagcgcc
     3721 gcctcctggc cgggcacgca cagcaccgac agagtgatcc ccggccggcc cttcttcatg
     3781 tgaactggag tgatccacgc gtccgcggca ccggcctcca gcagccgctc cagcacccac
     3841 ggggtcagct ccggcgtcat gtcgtcgatg ttggcctcca ggaccagggc gctgcccccg
     3901 agggcgccgt ccccgccagc gcccggggcg gcggcgccct ccacctgaat gcgctccccg
     3961 agtagcaccc gcagcaggtt gggcagctcg cgcaggcgcc gcccacccgc cccgtagccg
     4021 accgccgcca ccttgagcgg cggcagcgcc gcgaaccgtg ccgcattcgc cgccaggatc
     4081 gccgcgccgg tcggggtggt caactccacc tccacgccgc cgccgtagat gggcacgccc
     4141 cgcagcagct cgaccacggc gggcggcggc accggcagca cgcttgatgc atcgggggac
     4201 cccgcgctct cactgccgtg cccgccaccg ggcgtgcggc ccgtaccgac cgccaccggc
     4261 gaggcccaga cttcggtcac gccgagatcg tcgagcaaca gcgcggcacc caccacgtcg
     4321 gccaccgcgt cgaccgcgcc gacctcgtga aactggaccc gctctggcgg catccggtgg
     4381 acgcgcccct cggcctccgc gagcctggtg aaggtggaga gggcgcgggc ccgcaccggt
     4441 tctggcaggt cggcgccctc caggaccgca cgcagcgcgg cccagtcacg caccaccgac
     4501 ggcccggggg cgcgcaccac cacccg
//
LOCUS       DASWDC010000162         4333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_539408, whole genome shotgun sequence.
ACCESSION   DASWDC010000162 DASWDC010000000
VERSION     DASWDC010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539408"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VF995_05130"
     CDS             complement(<1..522)
                     /locus_tag="VF995_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9342981.1"
                     /translation="MLGELRIRDLGVIDDARLEVAPGLNVLTGETGAGKTMVVGALSL
                     LLGERADAGAVRAGQAAALVEGRLSLDGERDAIEALRASGADDEEGEVVVARQVLAEG
                     RSRAQLQGRMATVAAVAEVVRPLVEVHGQHEFQRLLRPAVQRDLLDQFAGGDVLALRA
                     DFAAGWRRLRQVRR"
     gene            complement(650..2251)
                     /locus_tag="VF995_05135"
     CDS             complement(650..2251)
                     /locus_tag="VF995_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX9342982.1"
                     /translation="MTTSAPTATPALTHRQIQVVYIGLMLAMLVAALDQTIVSTALPT
                     IVGSLGGFHGLSHLAWVSTAYLVTSTAAAVLFGKLSDLHDRKRTFQTALVIFLTGSVL
                     AGAARSLDQLIAFRALQGIGGGGLMALTTAVVADMVPPRQRGRYVGYLGGMLALATVA
                     GPLLGGFFVDHLTWRWIFYVNLPLGVAAFVVIGAVLHLPPKPHSGRRMDYRGAALLVA
                     AVSGAVLVASWGGTERPWGSPLIRGLSAATTLALGAFILVERRIGRRGGSPLLPLDLF
                     ANPVIAVTSVVGLIAGLVLFGSLLFLPLFLQVAKGASATSSGLLLAPLMAGVLAASIT
                     SGRLVTRYGRYKPFPVIGTALMTVAFWLLSLLHASTPRGVVVAEMLLLGAGVGLLMQV
                     LILAGQNAVQVRDLGLVTSLAQFFRELGGTFGVALFGAVFSARLGAELARHLPTTTAS
                     GLAATLHGAPAQLRQLPPAAHQALIAAVTHAVDSVFAWAIPFAAAAFVLSLFLQELPL
                     RDTHDFFAAMGDFDEAPEVKSEQPV"
     gene            complement(2248..2934)
                     /locus_tag="VF995_05140"
     CDS             complement(2248..2934)
                     /locus_tag="VF995_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9342983.1"
                     /translation="MSESGSRTSRTRLPRAERVQQILGAAARAFARSGYAATSMDEIA
                     AEAGISKLMIYRDFEGKRRLYEAILESAERRLNQLVAGDPQKGASDQGLVALLRLARE
                     QPDGFKLVFQQAAREPEFAAYADRFADEAIRTGERRLAEEIRDPAALHWAARVSFRLS
                     IEAVIAWLEVGDPARDEAFLSALRTLTGQPGRLAHDLAHAQPEPQPQSQAQDPDPSPQ
                     QHQDQGGTPA"
     gene            complement(3004..3921)
                     /locus_tag="VF995_05145"
     CDS             complement(3004..3921)
                     /locus_tag="VF995_05145"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX9342984.1"
                     /translation="MTSVAAGPGRPQRIGVVVHTGRPSALALGKQLVAWSAEHRVELR
                     TLAEERGCLGEELEVPCRPPEEFPEGLDLVVALGGDGTLLRAVGLALKAEVPVLGVNL
                     GRLGFLTEVEADGLPPALDAVWEGSYQVERRTTLAVRVLVEGTCVATDVAVNDVVLEK
                     AARERLAGVAVRLGGKLFARYAADGIIVASPTGSTAYSFSAGGPIVSPRLDALLLTPV
                     APHMVFNRSLVLHPDEEVQLEVLADGPSVVENIDGRAVRELAPGTVVEVRRGPYQALL
                     VRVEQPHFFGLVRSKFRLADAGETGSDDP"
     gene            complement(3974..>4333)
                     /locus_tag="VF995_05150"
     CDS             complement(3974..>4333)
                     /locus_tag="VF995_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9342985.1"
                     /translation="GVVAADLSFISLRLVLPTLAALLGPSGDGGLVLLVKPQFEAGRE
                     AVGKGGVVRDPAVRAAAVRSVAERAAALGLGVAGVCASPLPGPAGNVEFFLHLRAGAE
                     PLDEAALAAALAAEQPR"
BASE COUNT          643 a         1544 c         1628 g          518 t
ORIGIN      
        1 gcggcggacc tgccgcaggc ggcgccagcc ggcggcgaag tcggcgcgca gggcgagcac
       61 gtcgccgccg gcgaactggt cgagcaggtc gcgctggacg gccgggcgca gcagccgctg
      121 gaactcatgc tggccgtgca cctccaccag cgggcggacc acctcggcga ccgcggcgac
      181 ggtggccatg cggccctgca gctgcgcgcg gctgcgccct tcggcgagca cctgcctggc
      241 caccaccacc tccccttcct cgtcgtcggc gccgctggcc cgcagcgcct cgatcgcgtc
      301 gcgttcgccg tccaggctga gccgcccctc gaccagcgcg gcggcctggc cggcgcgcac
      361 cgccccggcg tcggcgcgtt cgccgagcag cagcgacagc gcgcccacca ccatggtctt
      421 gccagcgccg gtctcgccgg tgaggacgtt cagcccgggg gcgacctcaa gcctggcgtc
      481 atcaatcacg ccgaggtcgc ggatccgcag ctctcccaac atcaccgccg attctagccg
      541 cctcctgggg cagtcttccg cagttgtccc caggcggacg tcagcggagc gccgccaggt
      601 ggccggcggc gcttcgctgg gcagctcagg atagagagga cgaattggct cagacgggct
      661 gttcggactt cacttcgggc gcctcgtcga aatcgcccat ggcggcgaag aagtcgtggg
      721 tatcgcgaag gggcagctcc tgcaggaaca ggctcagcac gaaggcggcg gcggcgaacg
      781 ggatcgccca ggcgaacacg ctgtccacgg cgtgggtcac ggcggcgatg agcgcctggt
      841 gggcggcggg cgggagctgg cggagctgcg ccggggcgcc gtggagggtg gcggccaggc
      901 cactggcggt ggtggtgggc aggtggcggg ccagctcggc gccgagccgg gcagagaaga
      961 ccgcgccgaa cagggcgacg ccgaaggtgc cgccaagctc gcggaagaac tgcgccaggg
     1021 aggtcaccag gccaaggtcg cggacctgga cggcgttctg gccggcgagg atgagcacct
     1081 gcatgagcag gccgacgccg gcgccgagca gcagcatctc ggcgaccacc acgccacgcg
     1141 gggtgctggc gtggagcagc gacagcagcc agaacgcgac cgtcatgagg gcggtgccga
     1201 tcaccgggaa gggcttgtag cgcccgtagc gggtgaccag ccggccgctg gtgatgctgg
     1261 cggcgagcac cccggccatc agcggggcca gcagcaggcc cgagctggtg gcgctggcac
     1321 ccttggcgac ctgcaggaac agcggcagga acaggagcga gccgaacagc accagcccgg
     1381 cgatcaaccc gacgacgctg gtgaccgcga tcacagggtt ggcgaacagg tccaggggca
     1441 gcagcgggct gccgccgcgc cgcccgatgc gacgctcgac caggatgaag gcgccgagtg
     1501 ccagcgtggt cgcggcggac aggccgcgga tcagcggcga gccccagggg cgctcggtgc
     1561 cgccccagct cgccaccagc accgcgccac tgactgcggc gaccagcagg gccgcgccgc
     1621 ggtagtccat ccggcggccg ctgtggggct tcggcggcag gtgcaggacc gcgccgatga
     1681 ccacgaacgc ggcgacgccg agggggaggt tgacgtagaa gatccagcgc caggtgaggt
     1741 ggtcgacgaa gaagccgccg agcagcgggc cggcgaccgt cgccagcgcc agcatgccgc
     1801 cgaggtagcc gacgtagcgg ccccgctgcc ggggcgggac catgtcggcg acgaccgcgg
     1861 tggtgagcgc catcagcccg ccgccgccga tgccctgcag ggcgcggaag gcgatcagct
     1921 ggtcgagcga gcgggcggcc ccggccagca ccgagccggt caggaagatg accagcgcgg
     1981 tctggaaggt gcgcttgcgg tcgtgcaggt cgctgagctt gccgaacagg accgcggcgg
     2041 cggtcgaggt gaccaggtag gcggtcgaga cccaggcgag gtggctgagg ccgtggaagc
     2101 cgccgaggct gccgacgatc gtcggcaggg cggtggagac gatcgtctgg tcgagcgcgg
     2161 cgaccagcat ggcgagcatc aggccgatat agacaacctg gatctggcgg tgggtgaggg
     2221 cgggggtggc agtgggggcg gaggtggtca tgctggcgtg cctccctggt cctggtgctg
     2281 ctgcggcgat gggtccgggt cctgagcctg ggattggggc tgcggctcgg gctgggcgtg
     2341 ggcgaggtcg tgggcgaggc ggccgggctg gccggtgagg gtgcgcagcg cgctgaggaa
     2401 agcctcgtcg cgggcggggt cgccgacctc cagccaggcg atcaccgcct cgatcgacag
     2461 ccggaaggag acgcgggccg cccagtggag cgccgctggg tcgcggatct cctcggcgag
     2521 gcggcgctcg ccggtgcgga tcgcctcgtc ggcgaagcgg tcggcgtagg cggcgaactc
     2581 cggctcccgc gcggcctgct ggaacaccag cttgaagccg tcgggctgct cgcgggcgag
     2641 gcggagcagc gcgaccagtc cctggtcgga ggcgcccttc tgcgggtcgc cggcgacgag
     2701 ctggttgagg cggcgctccg ccgactcgag aatcgcctcg tagagccggc gcttgccctc
     2761 gaagtcgcgg tagatcatca gcttgctgat gccggcctcg gcggcgatct cgtccatcga
     2821 ggtggccgcg tagccgctgc gggcgaaggc ccgcgcggcc gcgccaagga tctgctggac
     2881 ccgctcggcc cggggaaggc gtgtccggct ggttcggctg ccgctttcgc tcacgcatgc
     2941 ctcctatact cttgagtatg aactgaagaa tacatactca aaagtatagt tgcaagagtt
     3001 tagttatgga tcgtcgctgc ccgtctcccc ggcgtcggcc agccggaact tggagcgcac
     3061 cagcccgaag aagtgcggct gctccacacg gaccagcagg gcctggtagg ggccgcggcg
     3121 gacttccacg acggtgccgg gggccagctc gcggacggcg cggccgtcga tgttctcgac
     3181 cacgctcggc ccgtcggcga ggacctcgag ctggacctcc tcgtcggggt gcagcaccag
     3241 cgagcggttg aacaccatgt ggggcgccac cggggtcagc agcagcgcgt caagccgagg
     3301 cgagacgatc ggcccgccgg ccgagaagct gtaggcggtc gatccggtgg gcgaggcgac
     3361 gatgatcccg tcggcggcgt agcgggcgaa cagcttcccg ccgagccgca ccgccacccc
     3421 ggcgagccgc tccctggccg ccttctccag caccacgtcg ttgacggcca cgtcagtggc
     3481 cacgcaggtc ccctccacca gcacccgcac ggccagggtg gtgcgccgct cgacctggta
     3541 gctgccctcc cagaccgcgt cgagcgcggg aggcagcccg tcggcctcca cctcggtgag
     3601 gaacccgagc cggccgagat tcacccccag caccggcacc tccgccttca gcgccaaccc
     3661 gaccgcgcgc agcagggtgc cgtcgccgcc aagcgcgacc accaggtcaa gcccctcggg
     3721 gaattcctcc ggcggccggc atgggacctc cagctcctcg ccgaggcagc cccgctcctc
     3781 cgccagggtg cgcagctcaa ccctgtgctc ggccgaccac gccaccagct gcttgccgag
     3841 cgcgagcgcc gacggccgcc cggtatggac caccacgccg atccgctgcg gccgtcccgg
     3901 ccccgccgcc accgaggtca ttcggcacgc tccgggatcg tcgtccaccg cagcggtgcg
     3961 ctcaccgtgg cgctcaccgt ggctgctccg ctgccagcgc ggccgccagt gcggcctcgt
     4021 ccaggggctc cgcgccggcc cgcaggtgca ggaagaactc cacgttgccg gccgggccgg
     4081 gcagcggcga ggcgcagact ccggcgacgc ccagcccgag cgccgcggcc cgttcggcga
     4141 ccgaacgcac cgccgctgcc cgcaccgcgg ggtcgcgcac caccccgccc ttgccgaccg
     4201 cctcccgccc ggcctcgaac tgtggcttga ccagcagcac caggccgccg tccccggacg
     4261 gtcccagcag ggcggccaag gtcggcagca ccagccgcag cgagatgaac gacaggtccg
     4321 ccgccaccac ccc
//
LOCUS       DASWDC010000163         7758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1207988, whole genome shotgun sequence.
ACCESSION   DASWDC010000163 DASWDC010000000
VERSION     DASWDC010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207988"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VF995_05155"
     CDS             <1..498
                     /locus_tag="VF995_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006351053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9342986.1"
                     /translation="PLEDVPGAAEMMAQMPPDLPMFKAAPPALFPDAQLGNRADILAA
                     DIPAGGKVSARAIARMYAALLGEVDGVRLLPPERLREATAVSSSGVDQVFGMPTTWAL
                     GYSVGGLGAGAQDNPTVFGVGGVGGSFAFGDTASGIAFAVTKNRLTPDFTAATQLSQL
                     VTSAL"
     gene            complement(521..1363)
                     /locus_tag="VF995_05160"
     CDS             complement(521..1363)
                     /locus_tag="VF995_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342987.1"
                     /translation="MVSSAPTVFALLDAEWAALATTVVPTHWHHQSALRLAEILQPTL
                     GDLLAFTERRDDPARSDQVLAALVWLAGGTSGPGGHDPLAARTVLQMLLPGAKALARR
                     LSWLGDAQERAAAVTATLYEQIRRYPIERRPARIAANLLADTHQRLLRRAGGAGRKPG
                     ETTAAGPILVPVSLEALAAHGEGSLPEAPPVAATPAEELLALLAWAVRNGYLTPAQGR
                     LIGQTRIGDAPCEELGARAGLGAHSLRRRRQRAEQALGRAAITHALGPNDLSRLLPLP
                     SRCA"
     gene            1665..2447
                     /locus_tag="VF995_05165"
     CDS             1665..2447
                     /locus_tag="VF995_05165"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9342988.1"
                     /translation="MSETSVLSALVSALRGGSVAVVDLTSPLSASTPAIQLPPEFGQT
                     ATFALEEISRYDDRGPAWYWNNFRSGEHTGTHFDAPNHWVTGKDLADVASVPASRLVA
                     PAAVLDFSAEAAKNPDFLVEIDHVKAWEAEHGPLPDGGWLLLRTGWDARSNSQQAFLN
                     ADERGPHTPGLSPECARWVAQDAPVIGLGVETVGTDAGAAHSFDPAFPCHSYLMGSDK
                     YGLTQLRNLDRLPPTGAVVVAGPLPIVTGSGAPARVLALVER"
     gene            2453..4108
                     /locus_tag="VF995_05170"
     CDS             2453..4108
                     /locus_tag="VF995_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9342989.1"
                     /translation="MKVADAVGRALAAAGVGQVFGVVGSGNFHLTNAMIAGGSRFVAA
                     RHEGGAATMADAYARMSGTVAALSVHQGCGLTNAMTGIAEAAKSRTPLLVVAAEVTEP
                     RSNFYVDQEALARAVGAVPVRVSTPETALGEARAAVATALRERRTVLLNLPLRLQTLD
                     APGAEAALAEVTPPEPPAPVEPSEADVAALTAVLDQARRPVFVAGRGARGPAARAALE
                     ALAERTGALLATSAVARGLFHGNPWSLDVSGGFASPLAAELIRGADLIIGWGCALNMW
                     TMRHGRLIAPDATVVQVDDDPSALGAHREIHLGVTGDVAVTAQRVLEMEAAGAEPRPG
                     CRSAALEAEIAARVRWRDVPYDDASTGERIDPRTLSIALDDLLPAERVVAIDSGNFMG
                     YPGMYLSVPDEQGFCFTQAFQSIGLGLATAIGAALAQPDRLPVAALGDGGALMSVADL
                     DTVRRLALPMVVVVYNDDAYGAEVHHFGPGGHPLDTVVFPPTDIAAVARGYGFEAVTV
                     RRPGDLKAVASWVAGERSAPLLIDAKVTSDRGSWWLEEAFRGH"
     gene            4236..4880
                     /locus_tag="VF995_05175"
     CDS             4236..4880
                     /locus_tag="VF995_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9342990.1"
                     /translation="MLERFTWFKQSAYRWDGDGLTLYIDPWEAEGPPADVILITHAHP
                     DHYQPDEIERLRKDGTRIVAPHDIARELSGDVTAVAPGDSVEVAGIKLQAVPAYNTAE
                     ERLEMHPKANNWVGYLLTLGGHDYYHAGDTDHLPELESVRTNVAFLPIGGFYCMDPDE
                     AAGLARAMAPDLAVPMHYGFVEGVGTPAFADRFARAAAPVPVRTLEPLRPFEQK"
     gene            4987..6012
                     /locus_tag="VF995_05180"
     CDS             4987..6012
                     /locus_tag="VF995_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9342991.1"
                     /translation="MAVPVVLVKVALPAHAPNPGPGPGGGGQASDHHSFQVVGRLRGL
                     ADLVAGDGTYPVPLPDGQTAWLFGDCRFRTAGGVAGVRNAILLQDAQRRFRMVTGHTG
                     ADPTDLIPRLRPNTWLWPSSGFVEHGHLMVFAEEFKGTLQPNGKVGFVATNHRYLVGL
                     ALPSLHPLPGPPHEVYGGPIAWGHTVLVNGSDVYVYGNLQQDHGWTNLTYLARFPLGA
                     STSYWRFWDGRDFVPYPAAAAPLRGSDGARLTTKLTSIIPNPDPQARAAGVGFAALTM
                     QPFGATFDLRTATRPQGPWGASRVLYKIPDPKKLAYLPRARPDPDGTLQLAYSVWASP
                     PRFLVIR"
     gene            complement(6090..7016)
                     /locus_tag="VF995_05185"
     CDS             complement(6090..7016)
                     /locus_tag="VF995_05185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9342992.1"
                     /translation="MPAQPPARSDLDSGNGPGHPIDAAYPNGARIWNYQLGGKDNFRI
                     DREASETASAMMQDIGAPTGEDAAQEGRHLLQRMVDAMLGQGIRQFLDLGSGFPNMAN
                     THQIAHRTDPAARVVYVDLDPVVSTHSAALMAGPNVVTLNADLREPQQVLGNQRVREL
                     LDFGEPVGILFVCMLHCLWDEEDPWAVVRAFRDAVVPGSALAISHLTDETRPQAAQQL
                     FQLTQDLHWSTPLICRSRADIMRFFDGFTLVEPGLVFPAQWRPELNNPLRVAGDHDRE
                     GEPVPVLQGAPPAQPGEGRGSDMHLCGVGIKD"
     gene            complement(7153..7539)
                     /locus_tag="VF995_05190"
     CDS             complement(7153..7539)
                     /locus_tag="VF995_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEX9342993.1"
                     /translation="MIRQAISADSAPPAIGPYSQAIVANGFVFCSGTAGIDPATGVPP
                     DGIEEQTEQALRNLDAILTAAGASLATVVKTTVFYANVDHFASINAVYARHLPDPPPA
                     RSAPANVNLPRNLLISIDAIAVLPAG"
BASE COUNT         1065 a         2852 c         2724 g         1117 t
ORIGIN      
        1 cccctggagg acgtgcccgg cgccgccgag atgatggccc agatgccgcc cgacctgccg
       61 atgttcaagg ccgccccgcc cgccctgttc cccgacgccc agctcggcaa ccgcgccgac
      121 atcctcgccg ccgacatccc cgcgggcggc aaggtctccg cccgggcgat cgcgcgcatg
      181 tacgccgcgc tgctcggcga ggtcgacggg gtccggctgc tgccgccgga gcggctgcgc
      241 gaggcgaccg ccgtgtcgtc cagcggcgtc gaccaggtct tcggcatgcc gaccacctgg
      301 gcgctcggct actccgtcgg cggcctcggc gccggcgccc aggacaaccc gacggtgttc
      361 ggcgtcggcg gtgtcggcgg cagcttcgcc ttcggcgaca ccgccagcgg catcgccttc
      421 gccgtcacca agaaccgcct cacccccgac ttcaccgccg cgacccagct cagccaactc
      481 gtcaccagcg cactctgaac tgccgccgga cggcggccag tcaggcgcag cgactcggca
      541 gcggcagcag gcgcgaaagg tcgttcgggc cgagggcgtg ggtgatcgcc gctcgcccga
      601 gtgcctgctc cgctcgctgc cggcgccgcc gcagactatg ggcgccgaga ccggcgcgcg
      661 cacccagctc ctcgcacggc gcgtcgccga tgcgggtctg cccgatcagc ctgccctgcg
      721 cgggcgtgag gtagccgttg cgcaccgccc aggccagcag cgccagcagc tcctccgccg
      781 gggtcgccgc aaccggcggc gcctccggca gcgagccttc gccgtgcgcg gccagcgcct
      841 ccagcgacac cggcaccagg atcggccctg cggcggtggt ctcgccaggt ttgcgcccag
      901 cacctccggc gcggcgcagc agccgctggt gggtgtcggc caggagattc gcggcgatgc
      961 gggcgggccg acgctcgatc ggatagcgtc ggatctgctc gtagagggtg gcggtcacag
     1021 cggcggcccg ctcctgcgcg tccccaagcc agctcaagcg gcgggcaagc gctttcgcgc
     1081 ccggcagcaa catctgcagc accgtccggg cggcgagcgg gtcatgcccg ccagggccac
     1141 tggtgccacc ggcgagccac accaaagcgg caagaacctg gtcgctccgg gcggggtcgt
     1201 cacggcgttc ggtgaaggcg agcagatccc cgagcgtggg ctggaggatc tcagcgagcc
     1261 gcagcgcgga ctggtggtgc cagtgcgttg gaacgacggt cgtggccagg gcggcccatt
     1321 cagcgtccag cagcgcgaac accgtggggg cggaggagac catgggctgg gtcctttcgg
     1381 gcgggggcgg tcacccgcac cgtcgcccac gcccctttcc gcggatccga cagcccgcct
     1441 gtcagccagg acctttcctt cgcctcaggt gagatgattg ccctgaccac actcgacact
     1501 ccccaggggt ggactgcgag ccggagccgc gtgaaccacc caggcccgga cggggtcccg
     1561 ttcacgcgac ctgtagtccg tagcagtgaa cgggacccca tccgggccaa cagcgccgca
     1621 gcgcagtctc gcacccgggg tatccctagc tcctggaggg agccatgtcc gagacgtcag
     1681 tgctctccgc gcttgtctcc gcgttgcggg gcggttcggt tgcggtggtc gacctcacct
     1741 cgccgttgtc ggcgtccacg ccggcgatcc agctgccgcc ggagttcggg cagacggcga
     1801 ccttcgcgct ggaggagatc agccgttacg acgaccgcgg cccggcgtgg tactggaaca
     1861 acttccgcag cggcgagcac accgggaccc acttcgacgc gccgaaccac tgggtcaccg
     1921 gcaaggacct cgccgacgtc gcctcggttc cggccagccg gctggtcgcc ccggcggcgg
     1981 tgctcgactt ctccgccgag gccgccaaga accccgactt cctggtcgag atcgaccacg
     2041 tcaaggcctg ggaggccgag cacggcccgc tgcccgacgg cggctggctg ctgctgcgca
     2101 ccggctggga tgcccggtca aactcgcagc aggcgttcct caacgccgac gagcgcggcc
     2161 cccacacccc cggcctgtcg ccggagtgcg ccaggtgggt cgcccaggac gcccccgtga
     2221 tcggcctcgg cgtcgagacg gtcggcaccg acgccggcgc cgcgcactcg ttcgacccgg
     2281 ccttcccctg ccactcctac ctcatgggca gcgacaagta cgggctcacc cagctccgca
     2341 acctcgaccg gctgccacct accggcgcgg tggtggtcgc ggggccgctg ccgatcgtca
     2401 ccggctcggg cgcgcccgcg cgggtgctgg ccctggtcga gcgctgagca cgatgaaggt
     2461 cgccgacgcc gtcggccgag cgctggcggc ggcaggggtc ggccaggtct tcggcgtggt
     2521 cggctccggc aacttccacc tgaccaacgc catgatcgcg ggcggctccc ggttcgtcgc
     2581 cgcccgccac gagggcggcg ccgccaccat ggccgacgcc tacgcccgca tgagcggcac
     2641 ggtggcggcg ctgagcgtgc atcagggctg cgggctcacc aacgcgatga ccggcatcgc
     2701 ggaggccgcg aagagccgca cgccgctgct ggtggtcgcc gccgaggtga ccgagccgcg
     2761 ctcgaacttc tacgtcgacc aggaggccct cgcccgcgcg gtgggcgccg tccccgtccg
     2821 ggtcagcacc cccgagaccg cgctggggga ggcccgcgcg gcggtcgcga ccgccctgcg
     2881 ggagcggcgc accgtgctgc tcaacctgcc gctgcggctc cagaccctcg acgcgcccgg
     2941 agcggaggct gccttggcgg aggtcacgcc gccggagccg ccagcgccgg tcgagccgag
     3001 cgaggccgac gtggcggcgc tcaccgcggt gctcgaccag gcccggcggc cggtcttcgt
     3061 cgccggacgg ggcgcgcgcg ggcccgccgc ccgcgcggcg cttgaggcgc tcgccgagcg
     3121 gaccggcgcg ctgctggcga cctcggcggt cgcccgcggc ctgttccacg gcaacccgtg
     3181 gtcgctggac gtctccggcg ggttcgcgtc gccgctggcg gccgagctga tccgcggcgc
     3241 cgacctgatc atcggctggg gctgcgccct caacatgtgg acgatgcggc acggccggct
     3301 gatcgccccg gacgccaccg tcgtccaggt cgacgacgac ccctcggccc tcggcgcgca
     3361 ccgcgagatc cacctcggcg tcaccggcga cgtggccgtg acggcccagc gggtgctgga
     3421 gatggaggcc gccggcgccg agccgcggcc aggctgccgg tcggccgcgc tcgaagccga
     3481 gatcgccgcg cgggtgcgct ggcgtgacgt cccctacgac gacgccagca ccggcgagcg
     3541 gatcgatccg cgcacgctga gcatcgccct ggacgacctc ctccccgccg agcgggtggt
     3601 cgcgatcgac tccggcaact tcatgggcta cccgggcatg tacctgtcgg tgccggacga
     3661 gcagggcttc tgcttcaccc aggcgttcca gtcgatcggc ctgggcctgg cgacggcgat
     3721 cggcgcggca ctcgcgcagc cggatcgcct gccggtcgcg gcgctcggcg acgggggcgc
     3781 cctgatgagc gtcgcggacc tcgacaccgt ccgccgcctc gctctcccga tggtggtcgt
     3841 ggtctacaac gacgacgcct acggcgcgga ggtccaccac ttcggccctg gcggccaccc
     3901 tctcgacacc gtggtgttcc ccccgaccga catcgccgcc gtcgcccgcg gctacggctt
     3961 cgaggcggtg accgtccgcc ggcccggcga cctgaaggcg gtcgcgtcct gggtggcggg
     4021 ggagcgatcg gcgccgctcc tgatcgacgc caaggtcacc agcgaccgcg gctcctggtg
     4081 gctcgaggag gcgttccggg gtcactgagc ggcgtgcggt gtgctctgcc ggaatctggg
     4141 gaactggaac ttcggcgccg cagcaacgat gatgctgtgc gacggtcccg gtccccgtct
     4201 ccgctggtcg tatcgattgg ttggaggaga ggcccatgct ggagcgcttc acctggttca
     4261 aacagtcggc ctaccggtgg gatggcgacg gcctgacgct ctacatcgac ccatgggagg
     4321 ccgagggccc gcccgccgac gtgatcctga tcacccacgc ccaccctgac cactaccagc
     4381 cggacgagat cgaacggctc cgcaaggacg gcacccggat cgtcgccccc cacgacatcg
     4441 cccgcgagct gagcggcgac gtcaccgcgg tcgcgccggg cgactcggtc gaggtcgccg
     4501 gcatcaagct ccaggcggtc ccggcctaca acaccgccga agagcgcctc gagatgcacc
     4561 cgaaggccaa caactgggtc ggctacctgc tgaccctcgg cgggcatgac tactaccacg
     4621 ccggcgacac cgaccacctc cccgagctgg agtcggtccg gaccaacgtg gccttcctgc
     4681 cgatcggcgg cttctactgc atggacccgg acgaggccgc cggcctggcc cgcgccatgg
     4741 cccccgacct ggccgtgccg atgcactacg gctttgtgga gggcgtgggc acgcccgcct
     4801 tcgccgaccg gttcgcccgc gcggccgcgc ccgtgccggt gcggaccctg gagcctctgc
     4861 ggcccttcga gcagaagtag ccgccgcagc ccgagggtcc aggattgaag gccgatgagt
     4921 caattgcgtt ccccagcgcg tcgccgcatt ccgctttgga tctgcctgcc cagcatcctg
     4981 ctggctgtgg cggtcccggt ggtcctggtc aaagtggcgc tgccagcaca cgctcccaac
     5041 cctggccccg gccctggcgg cggcggccag gcgagcgacc accacagctt ccaggtcgtg
     5101 ggtcggctgc gcggcctcgc cgacctggtt gccggcgacg gcacctaccc ggtgccgctg
     5161 cccgacggtc agaccgcctg gctgtttggc gactgtcgat ttcggaccgc tggcggcgtc
     5221 gccggcgtcc gcaacgcgat cctgctgcag gacgcgcagc gacggttccg catggtcacc
     5281 ggccacaccg gcgccgaccc gaccgacctg atcccaaggt tacggcccaa cacctggctg
     5341 tggccctcca gcggcttcgt cgagcacggc cacctgatgg tgttcgccga ggagttcaaa
     5401 gggacgctgc agccaaacgg caaggtcggc tttgtggcga ccaaccaccg ctacctggtg
     5461 ggtctggcgc tgccgagcct ccatccgctg ccggggccgc cgcacgaggt ctatggcggc
     5521 ccgatcgcct ggggccacac cgtgctcgtc aacggttcgg acgtctacgt ctacggcaac
     5581 ctccagcagg accacggctg gaccaacctc acctacctgg cccgcttccc ccttggcgcg
     5641 tcaaccagct attggcggtt ctgggacggc cgcgacttcg tcccgtatcc ggcagccgcc
     5701 gcgccgcttc gtggcagtga cggcgcccgg ctgaccacca agctcaccag catcatcccc
     5761 aaccccgacc cgcaggccag ggcggccggc gtgggttttg ccgcgctgac catgcagccg
     5821 ttcggcgcca ccttcgacct ccgcaccgcc acgcggccgc agggtccatg gggcgccagc
     5881 cgcgtcctct acaagatccc cgacccgaag aagttggcct acctgccccg cgcccgcccc
     5941 gaccccgacg gcacgctcca gctcgcctac tcggtctggg ccagcccgcc ccgcttcctc
     6001 gtcatcaggt agtccgcatc aggtagtccg ccccagcggg acagcgaccg cacggcgact
     6061 ccgatccggg aggcatgccc tcgcgcttgc tagtctttga tgccgacccc gcagaggtgc
     6121 atgtcggagc cgcggccctc cccaggctgc gcgggaggcg cgccttggag cacgggcacg
     6181 ggttcgccct cgcggtcgtg gtcgccagcg acacggagcg ggttgttcag ctcgggacgc
     6241 cactgcgcgg ggaacaccag cccaggctcg accagggtga agccgtcgaa gaaccgcatg
     6301 atgtcggctc ggctgcggca gatgagcggg gtgctccagt gcaggtcctg ggtgagctgg
     6361 aagagctgct gggcggcctg cggccgggtc tcgtcggtca ggtggctgat ggccagcgcg
     6421 ctgccgggta ccaccgcgtc gcggaacgcc cgcacgaccg cccaggggtc ttcctcgtcc
     6481 cacaggcagt gcagcatgca gacaaacagg atcccgaccg gctcgccgaa gtcgagcagc
     6541 tcgcggaccc gctggttgcc gagcacctgc tgcggctctc gcaggtccgc gttgagggtg
     6601 accacgttgg ggccggccat cagcgccgcc gaatgggtcg acaccaccgg atccaagtcg
     6661 acgtagacca ctcgcgcggc cgggtcggtg cggtgggcga tctggtgggt gttggccatg
     6721 ttcgggaagc cggagccgag gtcgaggaat tggcggatac cctgccccag catggcgtcc
     6781 accatccgct ggagcaggtg gcggccttcc tgggcggcgt cctcgccggt tggcgcgccg
     6841 atgtcttgca tcatcgcact ggccgtctcc gacgcctccc ggtcgatccg gaagttgtcc
     6901 ttgccgccca gctggtagtt ccagatgcgg gcgccgttgg gataggcagc gtcgatcggg
     6961 tggccgggtc cgttgccgga gtccagatcg gagcgtgcgg gtggttgggc tggcatcggc
     7021 gggttcctcc acgctgtgtt caagacgtga agatactagc cgtgattggg ggcaaatggg
     7081 agtgacgcct ccgttcagcg accgagcgcg gggtcacggc gaggcggcaa gcccatcttc
     7141 tggaaggtgc ggtcagccgg cgggcaggac agcgatcgcg tcgatggaaa tgagcaggtt
     7201 acgcggcagg ttcacgttgg ccggtgccga acgagccggc ggcgggtcag gcaggtgacg
     7261 tgcatagacg gcattgatcg atgcgaagtg gtcgacgttc gcatagaaga cggtggtctt
     7321 gaccacggtc gccagcgacg caccagcggc ggtgaggatc gcgtcaaggt tccgcagcgc
     7381 ctgctcggtc tgttcctcga taccgtccgg cgggacgccg gtggcggggt cgatgcctgc
     7441 tgtgcccgag cagaatacga acccgttggc gacgattgcc tggctgtagg gcccgatcgc
     7501 gggcggtgcg ctgtcggctg agattgcctg ccgaatcatc gctgaacctc acgtcatctc
     7561 ggcggtttta tcggcgtgcc tcgaacacga tgttgaaggg tgtctcggcg actcgccgga
     7621 agttgccgaa tccggcgtcg gtgacgatcc ggcggatcgg ctcctcgccg gctcggcgcg
     7681 cagcgcggcg accgcgagct ggaacgctcc cggggcgaag accgggccgt ctggattggt
     7741 cagtgcgaag gcctgctc
//
LOCUS       DASWDC010000164         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1623666, whole genome shotgun sequence.
ACCESSION   DASWDC010000164 DASWDC010000000
VERSION     DASWDC010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623666"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /gene="shc"
                     /locus_tag="VF995_05195"
     CDS             complement(<1..1637)
                     /gene="shc"
                     /locus_tag="VF995_05195"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX9342994.1"
                     /translation="MTVEKTTGADAGADLAAPAPASAAPPASVAGALERARDRLLALQ
                     HEQGWWKGELQTNVTMDAEDLLLREFLGVRTEEQTRRSANWIRSQQRADGTWATFYGG
                     PGDLSTTVESYAALRLAGDPPDAAHMRAAAAFILDGGGIERSRVFTRLWLALFGLWPW
                     EDLPVLPPEMLFLPSWVPLNIYDFACWARQTIVALTVVSAFRPVRPTSFALDELKTGL
                     PRPPIEPLRTWAGRFQRLDLALHAYQRRPVAPLRRAALRKATRWILDRQEADGGWGGI
                     QPPWVYSLIALNLMGYPLDHPAIRDGLAGLEGFTITEPEADERRLEACQSPVWDTCLA
                     LIALRDAGVAADDPALRRAADWMLGEEIRVRGDWAVRRPQLAPGGWAFEFANDHYPDI
                     DDTAEVVLALRRVAVGHPDQARLEAAVARGAAWTIGMRSRNGAWGAFDADNTSRLPLE
                     LPFCDFGEVIDPPSADVTAHVVEMLAELGMGSAEVTRGGVDWLLREQEPDGSWFGRWG
                     ANYLYGTGAAVPALVAAGMKPFHPAIVRAVRWLESVQ"
     gene            complement(1643..>2544)
                     /locus_tag="VF995_05200"
     CDS             complement(1643..>2544)
                     /locus_tag="VF995_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9342995.1"
                     /translation="GWVDADGHPADGSGGKAIRPALALLSAEAAGADAREGLPGAVAV
                     QLVHDFSLLHDDLMDGDEERRHRATAWKVFGPALAVLTGDALLALAGQVLFEVPGERG
                     AAAARHLGGATELLILGQAQDLDFEERMDVTVEECLAMTGNKTGALLACSASIGAVLA
                     GAPPATVDGLHAFGLHLGLAFQAVDDLLGIWGEPEVTGKPAWNDLRARKKSMPVTAAL
                     SAGGPSSERLAALLGGDGPIGEEDLSLAAKLVETAGGRDLTRAEAERQLTLALDRLGA
                     VGAPAAAHEQLAAVAHFVTTREL"
BASE COUNT          323 a          955 c          945 g          321 t
ORIGIN      
        1 tgctgcacgc tttccagcca gcggaccgcc cggacaatcg ccggatggaa cggcttcatc
       61 ccagcggcga ccagcgcggg gacggccgcg ccggtgccgt agaggtagtt ggcgccccag
      121 cggccgaacc aggagccgtc gggctcctgc tcgcgcagca gccagtcgac cccgcccctg
      181 gtcacctcgg cgctgcccat gccaagctcc gcgagcatct cgacgacgtg ggcggtgacg
      241 tcggcggaag gcgggtcgat cacctcgccg aagtcgcaga acggcagctc cagcggcagc
      301 ctggaggtgt tgtcggcgtc gaaggcgccc caggcgccgt tgcgggagcg catcccgatc
      361 gtccaggcgg ccccgcgggc gacggcggcc tccaggcgcg cctggtcggg gtggcccacg
      421 gcgacgcggc gcagcgccag caccacctcg gcggtgtcgt cgatgtcggg gtagtggtcg
      481 ttggcgaact cgaacgccca gccgccgggg gcgagctggg ggcggcgcac cgcccagtcg
      541 cctcggacgc ggatctcctc gccgagcatc cagtcggcgg cgcgccgcag cgcggggtcg
      601 tcggctgcca ccccggcgtc gcgcagcgcg atcagcgcca ggcaggtgtc ccacaccggc
      661 gactggcagg cctccaggcg ccgctcatcc gcctccggct ccgtgatcgt gaagccctcc
      721 aggcccgcca ggccgtcgcg gatcgccggg tggtcgagtg ggtagcccat caggttgagg
      781 gcgatcagcg agtagaccca tggcggctgg atgccgcccc agccgccgtc ggcctcctgc
      841 cggtcgagga tccagcgggt cgccttgcgc agggccgccc gccgcagcgg cgcgaccggc
      901 cgtcgctggt aggcgtgcag ggccaggtcg agccgctgga agcggcccgc ccaggtccgc
      961 agcggctcga tcggcggcct cggcaggccg gtcttcagct cgtcgagggc gaagctggtc
     1021 ggccggaccg ggcggaaggc gctgaccacc gtgagcgcca cgatggtctg ccgggcccag
     1081 caggcgaaat cgtagatgtt cagcggaacc cacgacggca ggaagagcat ctccggcggc
     1141 agcaccggca ggtcctccca cggccacagg ccgaacaggg cgagccacag ccgggtgaac
     1201 acccgcgagc gctcgatccc gccgccgtcg aggatgaagg cggcggcggc gcgcatgtgg
     1261 gccgcgtcgg gtgggtcgcc ggccagccgc agcgccgcgt acgactcgac ggtggtcgac
     1321 aggtcccccg ggccgccgta gaaggtcgcc caggtcccgt cggcgcgctg ctgggagcgg
     1381 atccagttgg ctgagcggcg ggtctgctcc tcggtgcgga ccccgaggaa ctcgcgcagc
     1441 agcaggtcct cggcgtccat ggtgacgttg gtctgcagct cgcccttcca ccagccctgt
     1501 tcatgctgga gcgcgagcag ccggtcgcgg gcccgctcca gtgcgcccgc cacggacgcg
     1561 ggcggagccg cgctcgcggg cgcgggcgcc gccaggtccg ctccggcgtc cgctccggtc
     1621 gtcttctcga cggtcatcgc cgctacagct cccgggttgt cacgaaatgg gcgacagcgg
     1681 ccagttgctc atgggccgcc gctggtgccc cgaccgcgcc gaggcggtcg agtgccagcg
     1741 tgagctggcg ttccgcctcg gccctggtaa ggtcccttcc ccctgctgtc tcgaccagct
     1801 tggcggccag ggagaggtct tcctcgccga tcgggccgtc gccgccgagg agggcggcga
     1861 gccgctcgga gctgggcccg ccggccgaca gcgcggcggt caccggcatg gacttcttcc
     1921 gcgcccgcag gtcgttccat gccggcttgc cggtcacctc tggttcgccc cagatgccca
     1981 gcaggtcgtc gaccgcctgg aacgccagcc cgagatgcag cccgaaggcg tgcaggccgt
     2041 ccacggtcgc gggcggggcg cccgccagca ccgcgccgat cgaggccgag caggcgagca
     2101 gcgcgccggt cttgttgccg gtcatcgcca ggcactcctc gacggtgacg tccatccgct
     2161 cctcgaagtc gaggtcctgg gcctggccga ggatcagcag ctcggtcgcg ccgcccaggt
     2221 gccgcgcggc ggcggcgccg cgctcgcccg gcacctcgaa caggacctgc ccggccagcg
     2281 cgagcagcgc gtcgccggtc agcaccgcca gcgccggccc gaacaccttc caggcggtgg
     2341 cgcggtggcg gcgctcctcg tcgccgtcca tcaggtcgtc gtggagcagc gagaagtcgt
     2401 gcaccagctg caccgcgacc gcgcccggca gcccctcccg ggcgtcggcg ccggcggcct
     2461 cggccgacag cagcgccagc gccgggcgga tcgccttgcc gccgctgccg tcggcggggt
     2521 ggccgtccgc gtcgacccag ccga
//
LOCUS       DASWDC010000165        10503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_939156, whole genome shotgun sequence.
ACCESSION   DASWDC010000165 DASWDC010000000
VERSION     DASWDC010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10503
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939156"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /gene="recR"
                     /locus_tag="VF995_05205"
     CDS             <1..521
                     /gene="recR"
                     /locus_tag="VF995_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX9342996.1"
                     /translation="ETADAERLARAIHEVKAKVHFCRICWNVAEGELCRICRDSRRDP
                     TITCVVEEPKDLVAIEKTQEFRGRYHVLGGAISPIEGVGPDDLRIRELLHRIEPEAIR
                     EVILATNPNIEGEATAMYLARLLSPLGVRVTRPASGLPVGGDLEYADEITLGRAFAGR
                     RDMSVEPGNSSR"
     gene            721..1995
                     /locus_tag="VF995_05210"
     CDS             721..1995
                     /locus_tag="VF995_05210"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX9342997.1"
                     /translation="MALIVQKYGGTSVGDAARIRRVADRVVATRRAGNDVVVVVSAMG
                     DTTDELVELATQVSANPPPRELDMLLTAGERISMALLAMAIAERGVTAKSFTGSQAGI
                     ITDTLHGKARILDVRAGRISEAIDAGHVVIVAGFQGVSTDHDVTTLGRGGSDTTAVAL
                     AAALGALACEIYTDVDGVYTADPRLVPEARKLHAVSYEEMLELAACGARVLMLRCVEY
                     ARRYGITLHVRSSFTDAQGTWVRQEDERMEKAIISGVPHDTSEAKVTMVRVPDRPGIA
                     AKILRGIADQGVNVDMIVQNVSHGGTTDISFTVPKADLPGAMVVLEKLGSEIGAVEVL
                     ADPDIAKVSLIGAGMKSSPGVAAAMFQALADAGVNIEMISTSTIRISCVIRAADVRVA
                     VRAIHDRFHLSDEAELRLDHAEGTAELPVVLP"
     gene            1992..3074
                     /locus_tag="VF995_05215"
     CDS             1992..3074
                     /locus_tag="VF995_05215"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX9342998.1"
                     /translation="MRARRLPAAVVVGATGAVGEDLRRIIAERELAVGEVRMVASERS
                     AGRRVAFRDEQVEVAPLAPEAFDGTAVAFFSAGGSVSREWAPVAAARGAVVVDNSSAW
                     RMHPQVPLVDADVNPEAALDHPLGIVANPNCTTLTITPVLAVLREMAGLLAITPTSFQ
                     AAGGSGQKAMDELAEQTGKLLGERELLRRGGEVSALPTGEVFPHPLAFNVIPQCMGFE
                     AAEPGYTVEELKLRDEPRKILGLPALASHPTCARVPVMVGHSVSVRALCERPVDLDAF
                     RAALATAAGVTVLDDPAASAYPTPLELAGSDGVFVGRIRRDLSDPNAVMFWAVADNLR
                     KGAALNAVQIGEVLLTAITMASGDMF"
     gene            3084..3779
                     /locus_tag="VF995_05220"
     CDS             3084..3779
                     /locus_tag="VF995_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9342999.1"
                     /translation="MVEDSMERGIAAIGLLHEPVRRALYRYVAAQAHEVSRNEAAQAL
                     GIQRGLAAFHLDKLVEAGLLEVASHRRLGGRSGPGAGRPAKLYRRSATEHELTLPPRR
                     YETAAHLLAEVVEASGTDLALYAAAKRHGEALGREATAGNPAASEADAEAVLAERGYE
                     PYREGATLRLRNCPFHSLSSEFPALVCGMNLALLEGLLRQLGPAGAELVARMVPRPGE
                     CCVALSPSKTSRN"
     gene            3812..4300
                     /locus_tag="VF995_05225"
     CDS             3812..4300
                     /locus_tag="VF995_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HEX9343000.1"
                     /translation="MDEQRYHLAQFNIGRILAPTDSPQLADFMALLEPINQLADASPG
                     FVWRLQTADGDATALRPYADDRMLINMSVWESLEAMRNFVYSSRHLDPLRRRREWFER
                     MTDHILVLWWVSAGHIPTVDEAKERLELLRRDGPGPEAFTFKEPYPDPRTLAGSERSL
                     RG"
     gene            complement(4321..4698)
                     /locus_tag="VF995_05230"
     CDS             complement(4321..4698)
                     /locus_tag="VF995_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEX9343001.1"
                     /translation="MIEFDPRSRLLWGSFPLRLSAIQRRSELFHVELSQLQATIEATF
                     GARDRARGIDGTFRWWVEEVGEVAKSLRRMNPAELEHEIGDALAWLASVANLAGVDLT
                     SAMRRYADGCPRCQHSPCTCPAA"
     gene            complement(4698..4774)
                     /locus_tag="VF995_05235"
     tRNA            complement(4698..4774)
                     /locus_tag="VF995_05235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4738..4740),aa:Pro,seq:cgg)
     gene            complement(4765..6051)
                     /locus_tag="VF995_05240"
     CDS             complement(4765..6051)
                     /locus_tag="VF995_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX9343002.1"
                     /translation="MTNPPGPDTSPSGRLARRLGTGDAVVIGLGSMLGAGVFAAFAPA
                     AAAAGSGLLFGLLLAALVAGCNATSSAELAAIYPSSGGTYVYGRERLGKFWGYLAGWA
                     FVSGKTASCAAMALTFGTYAAPRFARPLAVAAVAGLTAVNYRGIAKTAWLTRLLVALV
                     VAALAAAVTAALLGGQAEWAHLGPLGAGGPRGVLEAGGLLFFAFAGYARIATLGEEVV
                     DPERTIPRAIPRALTLVLVIYAVVALAALLTLGPGTLAHAHAPLAAAAGAGSLAWLVP
                     VVRAGAVVAALSSLLSLLVGVSRTTFAMAGGRDLPGWLDAVHPRYRVPHRAELAIAAL
                     VIVVLLAADLRGAIGFSSFNVLGYYAIANAAAWTLPPAQRRWPRWFAAAGAAGCVLLA
                     LSLPLTSIAAGAGVLAAGVLAWVLRGRYTAHAESGM"
     gene            6107..6547
                     /locus_tag="VF995_05245"
     CDS             6107..6547
                     /locus_tag="VF995_05245"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX9343003.1"
                     /translation="MGETRKRGTRATFHPGWEPFSLPEDEVLRRGAALFNDEMWWEAH
                     EVWEEVWRMRRDRPDRDFLKGMIQGAAGMWHLSQSNYKGAVSVLSRAVSYLEPYRPSK
                     DGVDVDALSEALREAVAASREALETGRPASVHMIKLELPPGASS"
     gene            6544..6855
                     /locus_tag="VF995_05250"
     CDS             6544..6855
                     /locus_tag="VF995_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HEX9343004.1"
                     /translation="MRMKASQSPQPQLPEMPTISMGEARARVRGRFEGDVRRIRIQPL
                     AAIPTLEVVLDDGSGRVTALFMGRRGIAGIECGRRLAVEGTPVAAERGLTLYNPAYEL
                     R"
     gene            complement(6871..7626)
                     /locus_tag="VF995_05255"
     CDS             complement(6871..7626)
                     /locus_tag="VF995_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX9343005.1"
                     /translation="MRVAIAGAGNVGQFIAADLLAAGHQVTLLEKDPTIIEAARNTLD
                     GAEWFPFDACEVLSLVMAGLSHADVVVAATGDDEDNLVISLLAKQEFAVPRVVARVNN
                     PKNHGLFNEAWGVDVAVSTPHILRSLVEEAVTIGSMVRLMQFEQGQVSLHEVTLAEGS
                     PSIGRTLRDLALPVDAAVVAVVREGHVVVPREDTVFAVGDEVLAVASEASERYVREAL
                     VGEARSRDEEQDQDDEQDQDEAQEEEQEPAERG"
     gene            complement(7623..8336)
                     /locus_tag="VF995_05260"
     CDS             complement(7623..8336)
                     /locus_tag="VF995_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX9343006.1"
                     /translation="MHVIIMGCGRVGSELAARLEARGHTVAVIDKREIAFRMLRFDFK
                     GQTVHGYGFDEDVLRRAGVEDAGAFAAVSNGDNSNIVAARLAREKFEVPIVVARIYDP
                     RRAEIYQRLGIPTVATVKWTTDQVMRFLIPDEIASDWKDPTEGISLVTLILPSAWAGW
                     TVEALEAPGHRRVTAVSRVGQARIVTPSTILQEGDQLYLAVDDDGLAELRPMIMEAHR
                     RSRAGVFGADRTGEGVRSS"
     gene            8596..>10503
                     /locus_tag="VF995_05265"
     CDS             8596..>10503
                     /locus_tag="VF995_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX9343007.1"
                     /translation="MAIEARRTAEPTPQGPGPEPVRGPAPGKVPSHRYQSLGYRTKRT
                     LLGPPLKTSQLLTERITKKVALAVFSSDPISSTAYATEEILLVLVTAVAASRFTVPVS
                     VAIAGLLLMLVISYRQVIEAYPSAGGAYVVSRDNFGTVVSAVAGAALLIDYILTVAVS
                     VSAGVAAMASVFRELEPWVVELSVAFVVLLAWGNLRGIKEAGRIFAIPTYVYVTSMAG
                     VVLLGIWRAVVGGLSPVAYQPDPSHPLFHAGEALSTVSFFLILRAFAAGTTALTGVEA
                     ISNGVSAFRRPEAKNAKTTLLTMALIMGSLFLGISYLAKRFGIKPYANGFPTVVAQLA
                     DHVLGQTVAGRVAFVIVQSATLLILVLAANTSFSGFPLLASFAAGDALLPRQLRKRGH
                     RLVYSNGIVVLSLIAIVLVIGFRARTSALIPLYAIGVVTSFTLSQAGMTVRHLRLRER
                     GWHYGIVVNGVGAVITFVVLGVIVVAKFTEGAWMVCLAIPILVLLLLRVQTTYRSEIK
                     ELKVEASQRLAPPKPRHEVVVLVEDLDRAVLAALQYAKQINPLSITAVHVAIDPDHAR
                     ELQHLWAKVHLPIPLEVVDAPDRNLLATVEETIAERVRPDTEVSVLIPKRSFSKWWHR
                     ILHDRTAQGVFK"
BASE COUNT         1541 a         3744 c         3706 g         1512 t
ORIGIN      
        1 tggagaccgc cgacgccgag cggctcgccc gcgcgatcca cgaggtcaag gcgaaggtcc
       61 acttctgccg catctgctgg aacgtggccg agggcgagct gtgccgcatc tgccgtgaca
      121 gccgccgcga cccgaccatc acctgcgtgg tcgaggagcc gaaggacctg gtcgccatcg
      181 agaagaccca ggagttccgc ggccgctacc acgtccttgg cggcgccatc agcccaatcg
      241 agggcgtcgg ccccgacgac ctccgcatcc gcgagctgct ccaccgcatc gaacccgagg
      301 ccatccgcga ggtcatcctc gccaccaacc ccaacatcga gggcgaagcc accgccatgt
      361 acctcgcccg cctgctctcc cccctcggcg tccgcgtcac ccgccccgcc agcggcctcc
      421 ccgtcggcgg cgacctcgaa tacgccgacg agatcaccct cggccgcgcc ttcgccggcc
      481 gccgcgacat gtccgtcgaa ccaggcaact cgtcgcgctg aaggccagcc tcatcgtcgg
      541 gatcgggtcg aagccgggga ggctggggtc gcgccgtaac catcgcatgt ctgactgaaa
      601 tcgctaatta cgctaactac ccgattcgct cagcttccct gcataatcaa taccgaggtg
      661 gctgaggcgg cttggtaggc tgggccgcac gtcgtcggat gcgaatctgt agggatgtca
      721 atggcactga tcgtccagaa gtacggcggt acctcggtgg gggacgcggc gcggatccgg
      781 cgcgtcgcgg atcgggtggt cgccaccagg cgggccggca atgacgtggt ggtggtggtc
      841 tccgccatgg gtgacaccac tgacgagctg gtcgagctgg ccacgcaggt ctccgccaac
      901 ccgccgccgc gcgagctgga catgctgctc accgcggggg agcggatctc gatggcgctg
      961 ctggcgatgg cgatcgccga gcggggggtg accgccaagt cgttcaccgg ctcgcaggcg
     1021 gggatcatca ccgacaccct gcacggcaag gctcgcatcc tcgacgtgcg cgccgggcgc
     1081 atcagcgagg cgatcgatgc cgggcacgtg gtgatcgtgg ccggcttcca gggggtctcc
     1141 accgaccatg acgtgacaac gctcggccgg ggcggctcag acacgaccgc ggtggcgctg
     1201 gcggccgcgc tgggcgcctt ggcctgcgag atctacaccg acgtggacgg ggtctacacc
     1261 gccgacccgc ggctggtgcc cgaggcgcgc aagctccacg cggtctccta cgaggagatg
     1321 ctggagctgg cggcctgtgg cgcccgggtg ctgatgctgc gctgcgtgga gtacgcgcgg
     1381 cgctacggca tcaccctcca cgtccgctcg agtttcaccg acgcccaggg gacctgggtg
     1441 cgccaggagg acgaacgtat ggagaaggcg atcatctccg gggtcccgca cgacacctcg
     1501 gaggccaagg tgaccatggt cagggtcccg gaccggccgg ggatcgccgc caagatcctt
     1561 cgcgggatcg ccgaccaggg cgtcaacgtg gacatgatcg tgcagaacgt cagccacggc
     1621 ggcaccaccg acatctcctt cacggtgccc aaggccgacc tgcccggcgc catggtggtg
     1681 ctggagaagc tcggcagcga gatcggcgcg gtcgaggtgc tcgccgaccc cgacatcgcc
     1741 aaggtgtcgc tgatcggcgc gggcatgaag tcgagcccgg gggtcgccgc ggcgatgttc
     1801 caggcgctgg ccgacgcggg cgtgaacatc gagatgatct cgacctcgac gatccgcatc
     1861 tcctgcgtga tccgcgccgc cgacgtccgg gtcgcggtgc gggccatcca cgaccgcttc
     1921 cacctctccg acgaggcgga gctgcgcctc gaccacgccg aaggcaccgc tgagttgccg
     1981 gtggttctgc cgtgagggcg cggaggcttc cggcggcggt ggtggtcggc gccaccggcg
     2041 ctgtcgggga ggacctgcgg cgcatcatcg ccgagcggga gctggccgtt ggcgaggtgc
     2101 gcatggtggc gtcggagcgc tcggccgggc ggcgggtggc gttccgggac gagcaggtgg
     2161 aggtcgctcc gctggcgccc gaggcgttcg acgggacggc agtggcgttc ttctcggctg
     2221 gcgggagcgt ctcgcgggag tgggcgccgg tcgccgccgc ccggggcgcg gtggtggtgg
     2281 acaactccag cgcctggcgc atgcacccgc aggtgccgct ggtcgacgcg gacgtcaatc
     2341 cggaggcggc cctcgaccat ccgctcggga tcgtcgccaa ccccaactgc accaccctga
     2401 ccatcacccc ggtgctggcg gtgctgcgcg agatggccgg gttgctggcg atcacgccga
     2461 cctcgttcca ggccgctggc ggctccgggc agaaggccat ggacgagctg gccgagcaga
     2521 ctggcaagct gcttggtgag cgcgagctgc tgcggcgcgg cggcgaggtg tccgcgctcc
     2581 ccacgggcga ggtgttcccg catccgctcg ccttcaacgt gatcccgcag tgcatgggct
     2641 tcgaggcggc cgagcccggc tacaccgtcg aggagctgaa gctgcgcgac gagccgcgca
     2701 agatcctcgg cctgccggcg ctcgccagcc atccgacctg cgcgcgggtg ccggtgatgg
     2761 tgggccactc cgtctccgtc cgggccttgt gcgagcgccc ggtcgacctg gacgccttcc
     2821 gcgcggccct ggccacagca gccggcgtca ccgtgctcga cgacccggcg gccagcgcct
     2881 acccgacccc gctggagctg gccgggagcg acggtgtctt cgtcggccgc atccggcggg
     2941 acctttccga ccccaacgcg gtcatgttct gggcggtggc cgacaacctc cgcaagggcg
     3001 ccgccctcaa cgccgtccag atcggcgagg tcctgctgac ggcgatcact atggcaagcg
     3061 gtgatatgtt ttagacttgc tccatggttg aggactcgat ggagcggggt attgccgcga
     3121 tcgggctgct gcacgagccg gtgcggcggg cgctgtaccg ctacgtggcc gcgcaggcgc
     3181 acgaggtgag ccggaatgag gcggcgcagg cgctcggcat ccagcgcggg ctggccgcct
     3241 tccacctcga caagctggtc gaggccggcc tgctggaggt cgcctcacac cggcggctcg
     3301 gcgggcgcag cggcccggga gccggccggc cggcgaagct ctaccggcgc tccgccaccg
     3361 agcacgaact gaccctgccg ccgcgccgct acgagaccgc cgcccacctg ctcgctgagg
     3421 tcgtcgaggc cagcggcacc gacctggcgc tgtacgccgc cgcgaagcgc cacggcgagg
     3481 cgctcggccg cgaggccacc gccggcaacc cggccgccag cgaggcggac gccgaagccg
     3541 tcctcgcgga gcgcggctat gaaccgtatc gggagggcgc gacactgcgg ctgcgcaact
     3601 gtcccttcca cagtctcagc agcgagttcc cagccctggt ctgcggcatg aacctggcgc
     3661 tgctcgaagg gctgctgcgg cagctcggcc cggctggcgc ggagctggtc gcgcggatgg
     3721 tcccccggcc gggagagtgc tgcgtcgcgc tctctccctc taaaaccagt aggaattgac
     3781 atagaggggg tcgccatgct ttgctcctgc tatggacgag cagcgctacc acctggccca
     3841 gttcaacatc ggccgcatcc tggcgccgac cgactccccg cagctcgccg acttcatggc
     3901 gctgctggag ccgatcaacc agctcgccga cgccagcccg gggttcgtct ggcggctgca
     3961 gaccgccgat ggcgacgcca ccgcgctgcg gccctacgcc gacgatcgca tgctcatcaa
     4021 catgtcggtg tgggagtcgc tggaggcgat gcgcaacttc gtctactcca gccgccacct
     4081 cgatcccctg cgccgccgcc gggagtggtt cgagcgcatg accgaccaca tcctggtcct
     4141 gtggtgggtc tcagccggcc acattcccac cgttgacgag gccaaggagc gcctggagct
     4201 gctgcgccgc gacggccccg ggcccgaggc gttcaccttc aaggagccat acccggaccc
     4261 gcgcacgctg gcagggagcg agcggagcct ccggggctga cggcagtcgt caggctgccc
     4321 tcaagccgcc gggcaggtgc agggggagtg ctggcagcga gggcagccgt cggcgtagcg
     4381 gcgcatggcg ctggtcaggt cgaccccggc gaggttggcg acgctggcca gccacgcaag
     4441 cgcgtcgccg atctcgtgct ccagctcggc ggggttcatc cggcgcaggg acttcgcgac
     4501 ctcgccaacc tcctcgaccc accagcggaa ggtcccgtcg atgccacgtg ctcggtcccg
     4561 cgctccaaag gttgcctcga tggtcgcctg cagctggctc agttccacgt gaaacaactc
     4621 cgatcgtcgc tgaatcgccg agagccgcaa cgggaacgac ccccacagca gtcggctgcg
     4681 ggggtcgaac tcaatcatgg tcgggatgcc gggatttgaa cccggggcct ccacgtcccg
     4741 aacgtggcgc gctgccaagc tgcgctacat cccgctctca gcgtgggcag tgtaccgccc
     4801 ccggaggacc catgcgagca cgccggcggc aagcacacca gccccggcgg cgatcgaggt
     4861 cagcggcagg ctgagggcga gcaggacgca gccagcggcc ccggcggcgg cgaaccagcg
     4921 gggccagcgg cgctgcgctg gagggagggt ccaggcggcg gcgttggcga tcgcgtagta
     4981 gccgagcacg ttgaaggagc tgaagccgat cgcgccgcgc aggtcggcgg cgagcagcac
     5041 cacgatcacc agggcggcga tggccagctc cgcccggtgg gggacgcggt agcgcgggtg
     5101 gacggcgtcg agccagccgg gcaggtcacg cccgccggcc atggcgaagg tggtgcggct
     5161 caccccgacc agcagcgaca gcagggagga gagcgcggcc accactgccc ccgcccggac
     5221 cactggcacc agccaggcga gcgaccccgc gccggcagcg gccgccagcg gcgcgtgggc
     5281 atgggcgagc gtcccgggcc cgagcgtgag cagcgcggcc agcgccacca cggcatagat
     5341 gaccaggacg agcgtgagcg cccgggggat ggctcgcggg atcgtgcgct cggggtcaac
     5401 cacctcctcg ccgagggtgg cgatgcgggc gtagccggcg aaggcgaaga acagcagccc
     5461 accggcctcc aggacgcccc gggggccgcc ggcgcccagc ggcccgaggt gcgcccactc
     5521 ggcctggccg ccgagcagcg cggccgtcac ggcggctgcc agcgccgcca cgaccagggc
     5581 gaccagcagc ctggtgagcc aggcggtctt ggcgatcccg cggtagttca cggcggtcag
     5641 cccggccacc gcggcgaccg ccagggggcg ggcgaaccgt ggcgcggcgt aggtcccgaa
     5701 ggtgagcgcc atcgcggcgc agctcgcggt cttgccgctg acaaaggccc agccggcgag
     5761 gtagccccag aacttcccga gccgctcgcg cccgtagaca taggtgccgc cagaggacgg
     5821 gtagatcgcg gccagctcgg ccgacgaggt cgcgttgcag cccgccacca gcgccgcgag
     5881 cagcaggccg aacagcagcc ccgaccccgc tgccgcggcg gccggcgcga acgcggcgaa
     5941 cacgcccgcg ccgagcatcg acccgaggcc gatcaccacc gcgtccccgg tgccaagccg
     6001 gcgcgccagc cggccgctgg ggctggtgtc ggggccgggc gggttcgtca tcgcgagtcc
     6061 agcatagaga cgccgcgaac cgggggcgcg ctgctacgat caggcaatgg gcgagacacg
     6121 caagcggggc acgcgggcga cattccatcc tggctgggag cccttcagcc tgccagagga
     6181 cgaggtgctg cggcgcggcg ccgcgctctt caacgacgag atgtggtggg aagcccacga
     6241 agtgtgggag gaggtctggc gcatgcgccg cgaccgcccc gaccgggact tcctcaaggg
     6301 catgatccag ggcgccgccg gcatgtggca cctcagccag agcaactaca agggcgcggt
     6361 gagcgtgctg agccgcgccg tcagctacct ggagccgtac cggccgagca aggacggcgt
     6421 ggacgtcgac gccctcagcg aagccctgcg cgaggcggtc gccgccagcc gcgaagcgct
     6481 ggagactggc cgcccggcga gcgtccacat gatcaagctg gagttgccac cgggagcgtc
     6541 gtcatgagga tgaaagccag ccagtccccg cagcctcagc tgcccgagat gcccaccatc
     6601 agcatggggg aggcgcgggc gcgggtacgc ggtcggttcg agggcgacgt gcggcgcatc
     6661 cgtatccagc cgctggcggc catccccacg ctggaggtgg tgctggacga cggcagcggc
     6721 cgggtgaccg ccctgttcat gggccggcgg gggatcgccg ggatcgaatg cggccgccgc
     6781 ctggcggtcg agggcacccc ggtcgccgcc gagcgcggcc tgaccctcta caaccccgcc
     6841 tacgaactcc gctagcggcg tggttcgacg ctatccccgc tcggccggct cctgctcctc
     6901 ttcctgcgcc tcgtcctggt cctgctcgtc gtcctggtcc tgctcctcgt cgcgactgcg
     6961 ggcctcgccg accagcgcct cgcggacgta gcgctcgctg gcttcggagg cgaccgccag
     7021 gacctcgtcg ccgacggcga agaccgtgtc ctcccggggg accaccacgt ggccttcgcg
     7081 taccacggcg accaccgcgg cgtcgaccgg cagggcgagg tcgcgcaggg tacggccgat
     7141 cgagggggag ccctcggcca gggtcacctc gtgcagtgag acctggccct gctcgaactg
     7201 catcaggcgc accatgctgc cgatggtgac cgcctcctcg accagcgagc gcaggatgtg
     7261 tggggtggac acggccacgt ccacgcccca ggcctcgttg aacagcccgt ggttcttggg
     7321 gttgttgacc cgggcgacta cccggggcac ggcgaactcc tgcttggcca gcagcgagat
     7381 caccaggttg tcctcgtcgt cgccggtggc ggcgaccacc acgtcggcgt ggctcaggcc
     7441 cgccatcacc agcgagagga cctcgcaggc gtcgaacggg aaccactctg ccccatccag
     7501 ggtgttgcgg gctgcctcga tgatggtcgg gtccttctcc agcagggtga cctggtggcc
     7561 ggcggcgagc aggtcggcgg cgatgaactg gccgacgttg ccggcgcccg cgatcgcgac
     7621 cctcatgagg agcgcacccc ctcgccggtg cggtccgccc cgaagacacc ggcgcggctg
     7681 cgccggtgtg cctccatgat catcgggcgc agctcggcca gtccgtcgtc atcgaccgcc
     7741 aggtagagct ggtcgccctc ctgcaggatg gtcgagggcg tcacgatccg ggcctggccg
     7801 actcggctga ccgcggtgac gcggcggtga cccggcgcct ccagcgcctc caccgtccag
     7861 ccggcccagg ctgatgggag tatcagggtg accagcgaga tgccctcggt cgggtccttc
     7921 cagtcggagg cgatctcgtc ggggatcagg aagcgcatca cctgatcggt ggtccacttg
     7981 acggtggcca cggtggggat gccaagccgc tggtagatct cggcgcggcg ggggtcgtag
     8041 atgcgggcga caacgatggg cacctcgaac ttctcccggg cgaggcgggc agccacgatg
     8101 ttcgagttgt ccccattgga aaccgccgcg aaggccccgg cgtcctcgac cccggcccgg
     8161 cgcaggacgt cctcgtcgaa gccatacccg tggacggtct ggcccttgaa gtcgaagcgc
     8221 agcatgcgga atgcgatctc acgcttgtcg atgaccgcga cggtgtggcc gcgggcctcg
     8281 agccgggcag ccagctcgga accgacgcga ccgcatccca tgatgatgac gtgcacggcc
     8341 ctgtccctgt ccttcctggt cgttccgcct ccgcctccgc cggcctctcc gtgggtatcg
     8401 ttgcttcgct tgcccgcacg ctcggccggc ggccgggctc gcagcatcct acgccgctcc
     8461 ctcgccgctc cgcgcccggt gaaaactctt gggacgggtt atccacagat tggcggcggc
     8521 cgtggtgcgg tccggttctg ggggcaatac tgggctagag gggggcagca cgaaatcagc
     8581 aaaggagcag aaccggtggc aatcgaggcg aggcggacgg ctgaaccgac gccgcaagga
     8641 ccaggacccg agccggtccg gggaccggcg ccgggcaagg tcccgtccca ccgctaccag
     8701 tcgcttggct accggacaaa gcggacgctt ctcggcccgc cgctgaagac ctcccagctc
     8761 ctcaccgagc gcatcaccaa gaaggtcgcc ctggcggtgt tctcctcgga cccgatctcc
     8821 tcgaccgcct acgccaccga ggagatcctg ctggtgctgg tgaccgccgt agccgccagt
     8881 cgcttcacgg tgccggtctc ggtcgccatc gccggcctgc tgctcatgct ggtgatctcc
     8941 taccggcagg tgatcgaggc ctacccgtcg gctggtggcg cctacgtggt cagccgtgac
     9001 aacttcggca cggtcgtgtc ggccgtcgcc ggcgccgccc tgctgatcga ctacatcctc
     9061 accgtcgcgg tgagcgtctc cgccggcgtc gccgccatgg cgtcggtgtt ccgtgagctg
     9121 gagccctggg tggtggagct gtcggtcgcc ttcgtggtgc tgctcgcctg gggcaacctg
     9181 cgcggtatca aggaggcggg ccggatcttc gcgatcccca cctacgtcta cgtcaccagc
     9241 atggccgggg tcgtcctgct cggcatctgg cgggcggtcg tggggggcct gagcccggtc
     9301 gcctaccagc ccgacccgag ccaccccctg ttccatgctg gtgaggcgct cagcacggtg
     9361 agcttcttcc tgatcctgcg cgccttcgcg gccgggacca ccgccctcac cggcgtcgag
     9421 gcgatctcca acggcgtctc ggcgttccgc cggcccgagg ccaagaatgc caagacgacc
     9481 ctgctgacga tggcgctcat catgggctcg ctgttcctcg gcatcagcta cctggccaaa
     9541 cgcttcggca tcaagccgta tgccaacggc ttcccgacgg tggtcgcgca gctcgccgac
     9601 cacgtcctcg gccagaccgt ggctgggcgc gtcgccttcg tgatcgtcca gtccgcgacg
     9661 ctgctgatct tggtcctggc tgccaacacc tcgttctcgg gattcccgtt gctggcctcg
     9721 ttcgccgccg gcgacgccct gctgccgcgc cagctccgca agcgcggcca ccggctggtc
     9781 tactcgaacg ggatcgtggt gctgtcgctg atcgcgatcg tgctggtcat cggcttccgg
     9841 gcgcggacct cggcgctgat cccgctgtac gccatcgggg tggtcacctc attcacgctc
     9901 tcccaggcgg gcatgaccgt ccgccacctg cggctgcgcg agcgaggctg gcactacggg
     9961 atcgtggtca acggcgtcgg tgccgtcatc accttcgtcg tgctcggcgt gatcgtcgtg
    10021 gcgaagttca ccgaaggcgc gtggatggtg tgcctggcca tccccatcct ggtcctgctg
    10081 ctgctacggg tccagacgac ctaccgctcc gagatcaaag agctgaaggt cgaggcctcc
    10141 cagcggctcg ccccacccaa gccccgccac gaggtggtcg tgctggtcga ggatctggac
    10201 cgcgcggtgc tggccgcgct gcagtacgcc aagcagatca acccgctgtc gatcaccgcg
    10261 gtacacgtcg ccatcgaccc cgaccacgcc cgcgagctgc agcacctgtg ggccaaggtg
    10321 cacctgccga tcccgcttga ggtggtcgac gcacccgacc gcaacctgct ggccacggtc
    10381 gaggagacga tcgccgagcg ggtccggccg gacaccgagg tgtcggtgct gatccccaag
    10441 cgctcgttct cgaagtggtg gcaccggatc ctgcacgacc ggaccgccca gggcgtcttc
    10501 aag
//
LOCUS       DASWDC010000166         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_187006, whole genome shotgun sequence.
ACCESSION   DASWDC010000166 DASWDC010000000
VERSION     DASWDC010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187006"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VF995_05270"
     CDS             <1..297
                     /locus_tag="VF995_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9343008.1"
                     /translation="GPSIIEALTYRHYGHSRTDPAKYRPKEELDAWLRRDPLLVLAAR
                     LRTRGVAAEEIERRQQAARRQVAAAIEAARAAPPPDEATAFTDVWADGGARWRT"
     gene            285..1265
                     /locus_tag="VF995_05275"
     CDS             285..1265
                     /locus_tag="VF995_05275"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX9343009.1"
                     /translation="MADLITYREAVAEGIAREMRRDPAVVLLGEDIGAAEGVFKTTAG
                     LFAEFGAERVWDTPISEQAIVGCATGAAMTGLRPMAEIMSSDFLACCWDYLANEIPKV
                     RYMTGGQLTVPLVVRTANGGGLGFGAQHSQAVENWALAVPGLKVCAPATPDDVIGLLA
                     SAIRSDDPVVFFEHKRLLASKGEAPPDGHLVPLGEAAVRRHGTDVTIAALAATVPTAL
                     NAAELLTAEGIDAEVIDLRCLVPLDAATVLASVERTSRLVIVEENPFQGGWGGTLASI
                     VADEGFDTLDAPIRRMAGACVPLPFAANLEAAVLPSANRLATAIQQLVNT"
     gene            complement(1314..1769)
                     /locus_tag="VF995_05280"
     CDS             complement(1314..1769)
                     /locus_tag="VF995_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018853607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9343010.1"
                     /translation="MRIVVDDLSGPEIARFLDDHVQEMRSITPPESKHALDLDGLRKP
                     EITFWSVMDGGTLAGCGAIKRLDAGHAEVKSMRTAPTRKRSGIASLLLKHIIAEAKRM
                     GFTRLSLETGAAEFFLPARKLYEKFGFSYCEPFADYRPDPHSVFMTRVV"
BASE COUNT          283 a          767 c          654 g          282 t
ORIGIN      
        1 ggcccgagca tcatcgaggc gctcacctac cgccactacg gccactcccg caccgacccc
       61 gccaagtacc gccccaagga ggagctcgac gcctggctgc ggcgtgaccc gctgctcgtg
      121 ctggccgcgc ggctgcggac ccgcggggtc gccgccgagg agatcgagcg ccgccagcag
      181 gcagcccgcc gtcaggtcgc cgccgcgatc gaggcggcca gggcggcacc gccgcccgac
      241 gaggcgacgg cgttcaccga cgtctgggcc gacggaggcg cgcgatggcg gacctgatca
      301 cctaccgcga ggcggtcgcc gagggcatcg cgcgagagat gcgccgcgac ccggccgtgg
      361 tcctgctcgg cgaggacatc ggcgccgccg agggcgtgtt caagaccacc gccgggctgt
      421 tcgccgagtt cggcgccgag cgggtgtggg acacccccat cagcgagcag gcgatcgtcg
      481 gctgcgccac gggcgcggcc atgaccggcc ttcggcccat ggccgagatc atgtcctccg
      541 acttcctggc ctgctgctgg gactacctcg ccaacgagat ccccaaggtt cgctacatga
      601 ccggcgggca gctgaccgtc ccgctggtgg tccgcaccgc caacggcggc gggctcggct
      661 tcggcgccca gcactcccag gcggtggaga actgggcgct ggcagtcccc ggcctcaagg
      721 tctgcgcgcc cgccacgccg gacgacgtga tcggcctgct ggccagcgcc atccgcagcg
      781 acgacccggt ggtcttcttc gagcacaagc gcctgctcgc cagcaagggc gaggccccgc
      841 cggacggcca cctcgtcccg ctcggcgagg cggcggtgcg ccgccacggc accgacgtca
      901 ccatcgccgc gctggccgcc accgtcccca ccgccctcaa cgccgccgag ctgctcaccg
      961 ccgaagggat cgacgccgag gtgatcgacc tgcgctgcct ggtcccgctg gacgcggcga
     1021 cggtgctcgc cagcgtggag cgcaccagcc gcctggtgat cgtcgaggag aaccccttcc
     1081 agggcggctg gggcggcacc ctggcctcca tcgtcgccga cgagggcttc gacaccctcg
     1141 acgcgcccat ccgccggatg gctggcgcct gcgtgcccct ccccttcgcc gccaacctcg
     1201 aggcggcggt cctcccatca gccaaccgcc tcgccaccgc catccaacag ctcgtcaaca
     1261 cctgacaggg caggcctctt cgccggcccg aacggtcgcc gcgctgacat ctctcaaacc
     1321 actcttgtca tgaagacgct gtgcgggtcg ggccggtagt ccgcgaacgg ctcgcagtaa
     1381 gagaacccga acttctcata cagcttccgg gcaggcagga agaactcggc cgcgccggtt
     1441 tccaggctca gccgtgtgaa ccccatgcgc ttggcctcag caatgatgtg cttcagcagc
     1501 agcgacgcga tcccgctccg cttgcgcgtc ggcgcggtgc gcatcgactt cacctcagcg
     1561 tggcccgcgt cgagccgctt gatcgcgccg cagcccgcca gcgtgccacc gtccatgacc
     1621 gaccagaacg tgatctcggg cttgcggagc ccgtcaagat cgagggcgtg cttgctctcc
     1681 ggcggcgtga tcgaccgcat ctcttggaca tggtcgtcga ggaacctggc gatctccggg
     1741 ccggagaggt cgtcgacgac gatcctcacg ttgcggacct ttcttcaggc ttctggtgcc
     1801 gcgggtgccc acgtcatccc cgggctcccc gccttctcca acctcacgat accgttgctc
     1861 gacgccacgc cccccggttc acggtggtgg cggtcgacgt gcctcccgtc atgcgtcggg
     1921 ggcgcgaagc accaccttgc cgcgcttccc acgcgcggtg acgcgctcga aggcggcgcg
     1981 ggcctg
//
LOCUS       DASWDC010000167         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_706538, whole genome shotgun sequence.
ACCESSION   DASWDC010000167 DASWDC010000000
VERSION     DASWDC010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706538"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..481
                     /locus_tag="VF995_05285"
     CDS             149..481
                     /locus_tag="VF995_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9343011.1"
                     /translation="MNADRHRCNDQQFITLHLGPLARTVIGSALVAAGGRPHGYQQTG
                     WGALTDTERKIANRLVLGRANAEIAEALFITTSTVEDHIGRIYAKLGIQRRQQLLASI
                     IRLASTSS"
     gene            530..1696
                     /locus_tag="VF995_05290"
     CDS             530..1696
                     /locus_tag="VF995_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX9343012.1"
                     /translation="MAVDPKAAVRERIEQAKDDLLGLSRRIHANPEIGFEEEQACGWL
                     CELLSDAGFQVEAGIAELPTAFVARSGPGPLRLVICAEYDALPGIGHACGHNIIAASS
                     AGAAIGLAGLAADLGIEVVVLGTPAEEGGGGKILLLERGAFAGAHAAMMVHPTPLDLT
                     AMPVIAVDQFTVRYTGRTAHASAWPELGINAADALVVAQTAIGLLRQHLRPTDRVHGI
                     VTKGGDAPNVVPADTSGRWLVRAASLEELDEVAQKVRRCFEAGALATGSTVEVTLDHV
                     PYAEMHHDPGLRAAYRRNAEAVGRRFPDDEGPYQRAAGSTDMGNVSLALPSIHPTIGI
                     DSLPAVNHQPEFTAACVTPAAEQALMDGAIALAWTAIDAASDEPLRGRLLAKSV"
     gene            complement(1717..2256)
                     /gene="tsaE"
                     /locus_tag="VF995_05295"
     CDS             complement(1717..2256)
                     /gene="tsaE"
                     /locus_tag="VF995_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX9343013.1"
                     /translation="MTVNELLLRAPDEAATRAIGAALALQLEPGDVVGLSGDLGAGKT
                     RLVQGAAAALGVEEPVVSPTFMLVREYDGRVPVHHVDAYRLSGAAELEDIGLDEVFPA
                     DAVVFVEWSARVEGALPDSWLELTLHTRDDEVREITVSAHGDGWVGRLPALAPALAQF
                     AVARAEPQPQPESEGQAKA"
     gene            complement(2261..2914)
                     /locus_tag="VF995_05300"
     CDS             complement(2261..2914)
                     /locus_tag="VF995_05300"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9343014.1"
                     /translation="MPTELELLAAEAATCTRCRLYESRTQVVFGQGSPTAEVMFVGEA
                     PGFHEDKQGLPFVGPSGQLLNRLLAGIGLRREDVYICNTVKSRPPNNRDPQADEIAAC
                     RPWLDGQIRLVDPNVVVTLGNFAAKTLLGTATGITRLRGRTYPFQGRVLLPTFHPAAA
                     LRAERGGRGSPTLQGMEEDFQHLAKLLAELRPSPPPSPEPGPEAGDTPGDGDQPGLF"
     gene            complement(2926..3156)
                     /locus_tag="VF995_05305"
     CDS             complement(2926..3156)
                     /locus_tag="VF995_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX9343015.1"
                     /translation="MPLYEYSCDACGQRFEALVPAGDADDATCTACGAGPVRRLLSLI
                     AAPVRGGDGAAAPAPMAGGGGGGCCGGACGHC"
BASE COUNT          470 a         1245 c         1118 g          416 t
ORIGIN      
        1 agcacctgcg cgtcggttgt gttgtatcga cccgcaggcg ggtgcggatg cgggcgccga
       61 cgggtggtgc ggcgatgaac ggctcggtga gggaacgcat gttcgagagc atagcaggca
      121 accatgacaa tgggcgtgag catcgctgat gaacgccgac cgtcacagat gcaacgacca
      181 acagttcata accctccacc tcggccccct ggcccgcacc gtgatcggct cggcactggt
      241 cgccgccggc gggcggccgc acggctacca gcagaccggc tggggggcgc tcaccgacac
      301 cgagcgcaag atcgccaacc ggctggtcct cggccgcgcc aacgccgaga tcgccgaggc
      361 gctgttcatc accaccagca ccgtcgaaga ccacatcggg cgcatctacg ccaagctcgg
      421 catccagcgc cgccagcagc tgctcgcctc gatcatccgg ctggcttcga cgtcctccta
      481 gtacctacga tggccgccga actggcaacc tcagcatgag gagacggcaa tggcagtgga
      541 cccgaaggcg gctgtccggg agcggatcga gcaggccaag gacgacctgc tggggctgag
      601 ccggcggatc cacgccaacc cggagatcgg cttcgaggag gagcaggcct gcggctggct
      661 ctgcgagctg ctgagcgacg ctgggttcca ggtggaggcc gggatcgccg agctgcccac
      721 cgccttcgtg gcgcgcagcg ggccggggcc gctgcggctg gtcatctgcg ccgaatacga
      781 cgccctcccg gggatcgggc acgcctgcgg gcacaacatc atcgccgcca gctccgccgg
      841 cgcggcgatc gggctggcgg ggctcgccgc cgacctcggc atcgaggtgg tggtgctggg
      901 aacccccgcc gaggagggcg gcggcggcaa gatcctgctg ctggagcgcg gcgccttcgc
      961 cggcgcccac gccgccatga tggtccaccc cacgcctctc gacctgaccg ccatgccggt
     1021 gatcgcggtg gaccagttca ccgtccgcta caccggccgg accgcgcacg cctccgcctg
     1081 gcccgagctc ggcatcaacg cggccgacgc gctggtggtc gcccagaccg cgatcgggct
     1141 gctgcgccag cacctgcgcc ccaccgaccg cgtccacggc atcgtcacca agggcggcga
     1201 cgcgcccaac gtggtccccg ccgacaccag cggccgctgg ctggtccgcg ccgcctcgct
     1261 ggaagagctg gacgaggtcg cccagaaggt gcgccgctgc ttcgaggccg gcgctctggc
     1321 caccggctcg acggtggagg tcaccctcga ccacgtcccg tacgcggaga tgcaccatga
     1381 cccgggcctg cgcgccgcct accgccgcaa cgccgaggcg gtcggccgcc gcttccccga
     1441 cgacgagggc ccctaccagc gtgccgccgg ctccaccgac atgggcaacg tctcgctggc
     1501 gcttccctcg atccacccga ccatcggcat cgactcgctg ccggccgtca accaccagcc
     1561 ggagttcacc gccgcctgcg tcaccccggc cgccgagcag gccctcatgg acggcgccat
     1621 cgccctggcc tggaccgcga tcgacgccgc cagcgacgag ccgctccgcg gccgcctcct
     1681 cgccaagagc gtctgaccca cacgaggccc tggccatcag gccttggcct gaccctcgct
     1741 ctcgggttga ggctgcggct cggcccgcgc gacggcgaac tgggccagcg ccggggccag
     1801 cgccgggagg cgcccaaccc agccgtcacc gtgggcgctg acggtgatct cccgcacctc
     1861 gtcgtcgcgg gtgtggaggg tcagctccag ccagctgtcc gggagggcgc cctcgacccg
     1921 ggcggaccac tcgacgaaca ccaccgcgtc ggcggggaag acctcgtcca gcccgatgtc
     1981 ctccagctcg gcggcgccgg agagccggta ggcgtcgacg tggtgcaccg gcacccgccc
     2041 gtcgtactcc cgcaccagca tgaaggtggg cgacaccacc ggctcctcca ccccgagggc
     2101 cgccgcggcc ccctgcacca gccgggtctt gccggcgccg aggtctccgc tgagccccac
     2161 cacgtcgccg ggttccagct ggagggcgag cgcggcgccg atcgccctgg tcgccgcctc
     2221 gtcaggcgcg cgcagcaaca gctcgttgac ggtcatgggc tcagaacagg cccggctggt
     2281 cgccgtcgcc cggggtgtcc cctgcttcgg gtcctggttc cggggacggg ggcggggacg
     2341 gccgcagctc ggcgaggagc ttggcgaggt gctggaagtc ctcctccatc ccctgcaggg
     2401 tcggcgagcc gcggccgccc cgctcggccc gcagcgcggc ggccggatgg aaggtcggca
     2461 gcaggacccg accctggaac ggataggtcc gcccccgcag ccgggtgatc cccgtggcgg
     2521 tcccaagcag cgtcttggcc gcgaagttgc ccagcgtgac caccacgttc ggatcgacca
     2581 gccggatctg cccgtccagc cacggccggc aggcggcgat ctcgtccgcc tgcgggtcgc
     2641 ggttgttggg aggcctcgat ttgacggtgt tgcagatgta gacgtcctcg cggcgcagcc
     2701 cgatgcccgc gagcagccgg ttgaggagct gcccggaggg cccgacgaac gggagcccct
     2761 gcttgtcctc gtggaagccg ggggcctcgc cgacgaacat cacctcggcg gtcgggctcc
     2821 cctgcccgaa caccacctgg gtgcggctct cgtacagccg gcagcgggtg caggtggccg
     2881 cctcggcggc gagcagctcc agttcggtcg gcatgagcgc cagcctcaac agtgcccgca
     2941 ggcgccgccg cagcagcccc cgcctccgcc tccggccatg ggcgcggggg ccgcggcccc
     3001 gtccccgccc ctgaccggcg cggcgatcag gctgagcagc cgccgcaccg gccccgcccc
     3061 acaggccgta caggtggcgt catcggcatc ccccgccggg acgagcgcct cgaaccgctg
     3121 cccgcaggcg tcacaggaat actcgtagag cggcacgcga cctccccctc catcgccttt
     3181 ccagcgactg aaggctatca ggtcacgcgc tccagctggc tggcccaaat tgagactaga
     3241 cgagacgtg
//
LOCUS       DASWDC010000168         6741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1291174, whole genome shotgun sequence.
ACCESSION   DASWDC010000168 DASWDC010000000
VERSION     DASWDC010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291174"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VF995_05310"
     CDS             <1..691
                     /locus_tag="VF995_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343016.1"
                     /translation="DPGAAACAALARLRRRWSRRWLAPVAFVLALLVILLATAVARPS
                     SSSSGHAALGAGTTARSRPAAGGGAASPAAAPAPAAAVPAGWGTFSDPGTGYRIASPP
                     GWHRETSGPYHRMVLADGDGRAFQIESARPPLGDPMALWTALAASFAKGHRGYHKIHL
                     GRGRYHGMSAAVWEFTHPQGGRTLHKVDVTFTSPKGQWGYAVLFTAPARLWPSSQVLV
                     RAFEQGFIPLE"
     gene            760..2529
                     /locus_tag="VF995_05315"
     CDS             760..2529
                     /locus_tag="VF995_05315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9343017.1"
                     /translation="MAQPETTGRRVAGRYELRGQLGEGGMGIIWHAEDLRLGRPVAIK
                     EIELPPAVSDKQRAFLRARVSREARAAARLNHPGAVIVYDVVSEDGQDYIIMELVDAP
                     TLSELVRERGPLEPSAAAELGLGVLATLRAAHEAGIVHRDVKPSNVMVRADGTTKLAD
                     FGVASLRGDPKLTSTGLVMGSPPYMAPEQVKSAEAGPATDLWSLGATLYFAVEGVPPF
                     GPGPQFQVLDAVVHKPPRRGKRLGTLAPVVSALLVKDPDQRPSPDDLRAMLRQVAAGA
                     KPLALPAPLAATATVPIAVPVTPPPGPSRDPHAEASAEASAEASREAVRPADRGAVQD
                     GSGDERLKGNGHAPNGAVAPPSPPPAPAAVPVPAPAGPEATWVGPAQQPPLPARSQAR
                     SRRRGLVLALLMLLGLVLAGLIGWRLGHSASASGAAKQRQRGRQVAAKPVVPADWVSW
                     TSPDRTWRVAHPPSWSLVHHFSGLVDFVVPGGARLFRVGVDQDQFDPLTVQRRLEASL
                     AAESNVRDYRRISLGLITYQHTRAAAWEYTYTGNGTPWRGLDVIFAEGGRRYAILFRA
                     TARQWGAATAERTGFLAAFKPLP"
     gene            2579..4201
                     /locus_tag="VF995_05320"
     CDS             2579..4201
                     /locus_tag="VF995_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9343018.1"
                     /translation="MPQHGDGGADGQDGRFTESGIPVEVCYGPDDLATAGFDPAAALG
                     EPGHDPYTRGIYPTMYRGRVWTMRQYAGFGSAAETNARFRYLLNHGQTGLSVAFDLPT
                     QMGYDSDHPMAAAEVGKVGVAIDSLADMRRLFEGIPLDRVSTSMTINAPAAILLLLYQ
                     LVGEQQGVDPAQLTGTVQNDVLKEYIARGTYIYPPAPSLRICTDLFAYCAEHLPRFNT
                     ISISGYHMAEAGADAVQEVAFTLSNAIAYVQAALDAGLEIDRFAPRLAFFFVARTTLF
                     EEVAKFRAARRLWADLMASRFGAQDPRSRMLRFHTQTAGVQLTAQQPQNNVVRVALQG
                     LAAVLGGTQSLHTNAFDEALALPSEHAARLALRTQQVLATETGVTDTVDPLGGSYFIE
                     RLTDEVEARARALMDEVEELGGAVAAIERGFQKERIERTAYDQLRAVERGDRVVVGVN
                     RHTEGEEEPVQLLQLDPAIAKAQTGSLAAIREQRDNHAVHAALTDLRDAAEGKDNLLV
                     PMREALRHLATLGEVCDTLREAFGVYRPPATF"
     gene            4272..4442
                     /locus_tag="VF995_05325"
     CDS             4272..4442
                     /locus_tag="VF995_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343019.1"
                     /translation="MSNTLQFILYLLAFLCFLLAAFGHRASWAGRLNLIGLGLALWVF
                     VPMVLELRKITS"
     gene            4500..5915
                     /locus_tag="VF995_05330"
     CDS             4500..5915
                     /locus_tag="VF995_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HEX9343020.1"
                     /translation="MRVVIIGGGPGGYEAALVAAELGAEVTIVSDEGLGGACVLWDCV
                     PSKTLLTSAQAFSTLETAPDLGVHFQGVPWHQVPTYEVDLPTIMRRVRELADSQSEDI
                     GKRVEADGVRFVVGKGSVADPHTVEVHDRDGGRLIERLSCDAILIATGSDPRTMATAP
                     TDGEVILCSQEVYRLKEVPERLVVVGAGATGAEYATAFLRFGSQVTFVVSRDRVLPGE
                     DEDAAMVIEEVFERRGLETVKNARAASVERTSAGGVVHLEDGRTVEGTHVLLTIGQVP
                     QSGGLGLERLGVGFNQSGGVVIDGVSRTSVPNIYAAGDVTGKLMLASVAAMQGRIAMW
                     HLLGQAVSPLRWDAVAATIFTDPQIATVGLSEQRAAKKDIDVVVHKLPLAGNSRAKMS
                     SLHDGFVKLLARPGSGTIVGGAVIAGYASDLIASISAACFNRLTAAQLSQAFQIYPSF
                     AGSIQECARLLAGRIQGTYRY"
     gene            6337..>6741
                     /locus_tag="VF995_05335"
     CDS             6337..>6741
                     /locus_tag="VF995_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HEX9343021.1"
                     /translation="MRPEDTHPLPELVFAQASPRSVGGTSLFEAGPVTQDMAADFISD
                     PELLRRTAEQLAAAGFQVLNVSAQTVNIAGAPALYEAYFQTQLRTEERPVLKPGQLED
                     TATFIESSSTDLPGLIDAARSPAAELLEGVAIE"
BASE COUNT          960 a         2502 c         2331 g          948 t
ORIGIN      
        1 agatcccggt gccgccgcgt gcgctgcgct ggcgcggctg cggcggcgct ggtcgcggcg
       61 ctggctggcg ccggtggcgt tcgtgctggc gctgctggtg atcctgctcg ccaccgcggt
      121 cgccagaccc tcgtcctcga gcagcggcca cgcggcgctg ggagcgggga cgacggcgcg
      181 gagcaggccg gcggccggtg gcggcgccgc gtcgcctgcg gcggcgcccg cgcccgcggc
      241 ggcggtcccg gcggggtggg ggaccttcag cgaccccggg accgggtacc ggatcgcctc
      301 cccgcccggg tggcaccgcg agacgagcgg cccctaccac cgcatggtgc tggccgacgg
      361 cgacgggcgg gcgttccaga tcgagtcggc caggcccccg ctcggcgacc cgatggcgct
      421 gtggaccgcg ctggcggcga gcttcgccaa gggccaccgc ggctaccaca agatccactt
      481 gggccgcggc aggtaccacg gcatgtccgc cgcggtgtgg gagttcaccc atccgcaggg
      541 cggccgcacc ctccacaagg tcgacgtcac cttcaccagc ccgaagggcc agtggggcta
      601 cgcggtgctg ttcaccgccc cggcccgcct gtggccctcc tcccaagtcc tggtgcgcgc
      661 cttcgagcaa ggcttcatcc cgctcgaata gcctgaaact acgccaatgg gcgtttcgcg
      721 gatggtcatt ccggcccaga gccggtagcg ttcggcgcta tggcgcaacc agagacgacc
      781 gggcgccggg tcgccggccg ctacgagctg cgggggcagc tcggcgaggg cggcatgggc
      841 atcatctggc acgccgagga cctgcggctc ggccggccgg tggcgatcaa ggagatcgag
      901 ctgccgccgg cggtcagcga caagcagcgc gccttcctgc gggcacgggt ctcgcgggag
      961 gcgcgcgccg ccgcccggct caaccacccc ggggcggtga tcgtctacga cgtcgtctcc
     1021 gaggacggcc aggactacat catcatggaa ctggtcgacg caccgaccct gagcgagctg
     1081 gtgcgcgagc gcgggccgct ggagccgtcg gcagcggccg agcttggcct cggggtgctc
     1141 gccaccctgc gggcggcgca cgaggcgggg atcgtgcacc gcgacgtcaa gccgagcaac
     1201 gtcatggtgc gcgccgacgg caccaccaag ctggccgact tcggcgtcgc ctcgttgcgc
     1261 ggcgacccca agcttacctc caccgggctg gtcatgggct cgccgcccta catggccccg
     1321 gagcaggtca agagcgcgga ggccggaccg gccaccgacc tgtggtcgct cggtgccaca
     1381 ctgtatttcg ccgttgaggg cgtgccgccg ttcgggcccg gcccgcagtt ccaggtcctg
     1441 gacgcggtgg tccacaagcc gccccggcgc ggcaagcggc tcggcacgct cgccccggtg
     1501 gtcagcgcgc tcctggtcaa ggatcccgac cagcggccca gccccgacga cctgcgagcc
     1561 atgctgcggc aggtcgccgc tggcgccaag ccgctggcgc tccccgcacc gctcgccgcc
     1621 accgccaccg tcccgatcgc cgtgccggtg accccgccgc ccgggccgtc ccgcgacccg
     1681 cacgccgaag ccagtgccga agccagtgcc gaagccagca gggaggctgt gcggccagcc
     1741 gatcggggtg ccgtccagga tggcagcggg gacgagcggc tgaagggcaa cggtcacgct
     1801 cccaacgggg cggtggcgcc cccgtccccg cccccagccc cggcagcggt gccggtgccg
     1861 gcaccggcgg ggcccgaggc gacctgggtg ggcccggcgc agcagcctcc gctcccggct
     1921 cggtcccagg cccgctcgcg ccgccggggg ctcgtcctgg cgctgctaat gctgctcggg
     1981 ctggtcctcg cgggactgat cggctggcgc ctcggccaca gcgcctcggc ctcgggcgcc
     2041 gccaagcagc ggcagcgagg ccgtcaggtc gcggccaagc cggtggtccc ggccgactgg
     2101 gtcagctgga ccagccccga ccgcacctgg cgggtcgcgc acccgccaag ctggtcgctg
     2161 gtccatcact tcagcggcct ggtcgatttc gtcgtcccag gcggtgcgcg cctcttccgc
     2221 gtcggcgtgg accaggacca gttcgacccg ctcaccgtcc agcggcggct tgaggccagc
     2281 ctggccgccg agtcgaacgt ccgcgactac cggcgcatca gcctcggcct gatcacctac
     2341 cagcacaccc gcgccgccgc ctgggagtac acctacaccg gcaacggcac gccgtggcgc
     2401 ggcctggacg tcatcttcgc cgagggcggg cgccgctacg cgatcctgtt tcgcgccacc
     2461 gcaaggcaat ggggcgcggc cacggccgag cggaccggct tcctcgccgc cttcaagccg
     2521 ctgccctgac ggctgcgcta cgctcgcgtc tcgcgaccgg cgcaggaggg cagcgacgat
     2581 gccgcagcat ggcgatggcg gagccgacgg tcaggacggg cgcttcaccg aatcgggaat
     2641 cccggtcgag gtctgctacg ggccggacga cctggccacg gccggcttcg acccggccgc
     2701 cgcgctcggc gaaccagggc acgaccccta cacccgcggg atctacccga ccatgtaccg
     2761 cgggcgggtc tggaccatgc gccagtacgc cgggttcggg tcggccgccg agaccaacgc
     2821 gcgcttccgc tacctgctga accacggcca gaccgggctt tcggtcgcct tcgacctgcc
     2881 cacccagatg ggctacgact ccgaccaccc gatggccgcc gccgaggtcg gcaaggtcgg
     2941 cgtcgcgatc gactcgctgg ccgacatgcg gcgcctgttc gagggcatcc cgctggaccg
     3001 ggtctccacc tcgatgacca tcaacgcgcc cgccgcgatc ctgctgctgc tctaccagct
     3061 cgtcggcgag cagcaggggg tcgatcccgc gcagctcacc ggcacggtcc aaaacgacgt
     3121 gctgaaggag tacatcgccc gggggaccta catctacccg cccgcgccga gcctgcggat
     3181 ctgcaccgac ctgttcgcct actgtgccga gcacctgccg cgcttcaaca ccatctcgat
     3241 ctccggctac catatggccg aggccggcgc ggacgcggtg caggaggtcg ccttcacgct
     3301 ctccaacgcg atcgcctatg tccaggccgc cctcgacgcc gggctggaga tcgaccgctt
     3361 cgcgccccgg ctggcgttct tcttcgtggc gcgcaccacg ctgttcgagg aggtcgccaa
     3421 gttccgcgcc gctcggcgac tgtgggccga cctgatggcc agccgcttcg gcgcccagga
     3481 cccgcgctcg cggatgctcc gcttccacac gcagaccgcg ggggtgcagc tcaccgccca
     3541 gcagccgcag aacaacgtcg tccgggtggc gctccagggc ctcgccgcgg tcctcggcgg
     3601 cacccagtcg ctgcacacca acgcctttga cgaggcgctg gcgctgccga gcgagcacgc
     3661 cgcgcggctg gcgctgcgca cccagcaggt cctcgccacc gagaccggcg tcaccgacac
     3721 cgtcgacccg ctcggcgggt cctacttcat cgaacgcctc accgacgagg tcgaggcgcg
     3781 ggcccgcgcg ctcatggacg aggtcgagga gctcggcggc gcggtcgccg cgatcgagcg
     3841 cggcttccag aaggagcgca tcgagcgcac cgcctacgac cagctccgcg ccgtcgagcg
     3901 cggcgaccgg gtggtcgtcg gcgtcaaccg ccacaccgag ggcgaggagg agccggtcca
     3961 gctcctccag ctcgacccgg ccatcgccaa ggcccagaca ggctcgctgg ccgccatccg
     4021 cgagcagcgc gacaaccacg cggtccacgc cgccctcacc gacctccgcg acgccgccga
     4081 gggcaaggac aacctcctgg tcccgatgcg cgaggccctc cgccacctcg ccaccctcgg
     4141 cgaagtctgc gacaccctcc gcgaagcctt cggcgtctac cgcccgcctg ccaccttctg
     4201 acgggtttct tgggtccttt cgccggagat gtgagtgtca ggtcttgcct tctccgaaag
     4261 gaccagcgct catgtccaac accctccagt tcatcctgta tctccttgcc tttttgtgct
     4321 tcctgctggc cgccttcggc caccgagcct cctgggcggg ccgcctcaac ctgatcggcc
     4381 tcggccttgc cctctgggtc ttcgtgccca tggtgctgga actgagaaag atcacctcgt
     4441 agccgagggc ctgccgaacc agagacattg ccaccaggcc tggcgggtac catctgccta
     4501 tgcgtgtcgt gatcattggg ggagggccgg gcggctatga ggccgcgctg gtcgccgccg
     4561 agcttggggc cgaggtgacg atcgtctcgg acgaggggct cggcggggca tgcgtgctgt
     4621 gggactgcgt gccgtccaag acgctgctga cctcggcgca ggcgttttcg acgctggaga
     4681 ccgcgccgga cctgggggtg cacttccagg gggtgccgtg gcatcaggtg ccgacctatg
     4741 aggtggacct gcccaccatc atgcggcggg tccgggagct ggccgactcg cagtccgagg
     4801 acatcggcaa gcgggtcgag gccgacgggg tgcggttcgt ggtcggcaag ggctctgtcg
     4861 ccgacccgca caccgtggag gtgcacgacc gggacggcgg gcggctcatc gagcggctct
     4921 cctgcgacgc gatcctgatc gccaccgggt cggacccgcg caccatggcg accgcgccga
     4981 ccgacgggga ggtgatcctc tgcagccagg aggtctaccg gctgaaagag gtgccggagc
     5041 ggctggtggt ggtcggggcg ggggccaccg gggcggagta cgccaccgcg ttcctgcgct
     5101 tcggctcgca agtcaccttc gtggtcagcc gcgaccgggt gctgccgggc gaggacgagg
     5161 acgccgccat ggtcatcgag gaggtgttcg agcggcgcgg ccttgagacc gtcaagaacg
     5221 cccgcgccgc ctcggtcgag cggaccagcg ctggcggcgt cgtccatctg gaggacggcc
     5281 gcaccgtcga gggcacccat gtgctgctga ccatcgggca ggtgccgcag agcggcggcc
     5341 tcggcctgga gcggctcggc gtcggcttca accagagcgg cggggtggtc atcgacgggg
     5401 tgagccgcac ttcggtcccg aacatctacg cggccggcga cgtcaccggc aagctgatgc
     5461 tcgcctcggt cgcggcgatg cagggccgca tcgccatgtg gcacctgctc ggccaggcgg
     5521 tctcaccgct gcgctgggac gcggtcgccg ccaccatctt caccgacccg cagatcgcca
     5581 ccgtcggcct ctccgagcag cgcgccgcca agaaggacat cgacgtcgtg gtccacaagc
     5641 tccccctggc cggcaactcc cgcgccaaga tgagcagcct ccacgacggc ttcgtcaagc
     5701 tcctcgcccg tcccggctcc ggcacgatcg tcggcggcgc cgtgatcgcc ggctacgcct
     5761 ccgacctgat cgcctccatc tccgccgcct gcttcaaccg tctcaccgcc gcccagctct
     5821 cccaggcctt ccagatctac ccgtccttcg ccggctccat ccaggagtgc gcccgcctcc
     5881 tcgccggccg catccagggc acctaccggt actgaacagc cattgcggcg aggaagagga
     5941 acacgccatg gagccaagca ttcccgattt cattgagctt gccggatcgg tgcatgtccc
     6001 cgaggagctg cgcgacctgt catgggagga gatacgagag cgcgcgtgga aagctcgggg
     6061 cgaggagctt gaggttctga gtcgtactga gacatccaag caggaccgga cacgcgactt
     6121 cccttctccg agtaattgag gtaaaaggga cgcaacatcc acgccacatt gagtggcccg
     6181 ggcgagtgca gcaacactcc ccgggccgtg gccgacaccc cacagaaggt gccgacatgg
     6241 gcaacgttac ccccatccct ctcatcccgg ttagtccgtt caacccgccg gaacggtgac
     6301 cgaggacttc gaccgtcgca ggcgcggagc ttggcgatga gacccgagga cacccatccg
     6361 ctgccggagc tggtgttcgc ccaggcgtct ccccggtcgg tcggggggac gtcgttgttc
     6421 gaggcggggc cggtcaccca ggacatggcg gcggacttca tctccgatcc ggagctgctg
     6481 cggcggacgg cggagcaact ggccgcagcc gggttccagg tgctgaacgt ctcggcgcag
     6541 acggtcaaca tcgccggggc gcccgccctc tacgaggcgt acttccagac ccagctgcgc
     6601 actgaggagc gcccggtgct caagccgggg cagctggagg acaccgccac cttcatcgag
     6661 tcgagcagca ccgacttgcc cgggttgatc gacgcggcgc gcagcccggc ggcggagctg
     6721 ctggaggggg tcgcgatcga g
//
LOCUS       DASWDC010000169         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1374086, whole genome shotgun sequence.
ACCESSION   DASWDC010000169 DASWDC010000000
VERSION     DASWDC010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374086"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1193
                     /locus_tag="VF995_05340"
     CDS             39..1193
                     /locus_tag="VF995_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX9343022.1"
                     /translation="MPDARLLSVILIVCVAVLFDYSNGFHDAANSIATVVSTRVLTPR
                     IAVIWAAAFNFLAFLVFHVLFHTSVANTVGKSVSPAIVSEAVVFAGLAGAVIWNFTTW
                     WFGLPTSSSHALIGGFVGAGVAKAGWHVVNSASLRKTVLFIVLSPLFGLVFAFLLMFV
                     LMWVFHRSSPRRVDRLFRRLQLFSAAAFSLGHGGNDAQKTMGVVSALLVGAGYLHLNA
                     NGDLPLPFWVVLLAYAAIAAGTATGGWRIVKTLGQKITALKPIGGFSAETAAAISIYI
                     ATFLGIPVSTTHTITGAIVGAGSTRRLSAVRWGVAGRIVWAWIITIPAAGALAAVAYW
                     LVAQQNHLIPLLALAALLVLSGALVAARRTPQPSAEPGTGARVSAGAGSD"
     gene            1236..1493
                     /locus_tag="VF995_05345"
     CDS             1236..1493
                     /locus_tag="VF995_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9343023.1"
                     /translation="MRVMTATEASRHFSDLLDAVERGETVRIMRGSHPVAEIGPAPRR
                     TGADLSAALAGVEPLDEDFASDIAAALALVNSEVPDPWADA"
     gene            1478..1876
                     /locus_tag="VF995_05350"
     CDS             1478..1876
                     /locus_tag="VF995_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9343024.1"
                     /translation="MGRRLILDTNMLIAYERGSFDRSTLRDDELAVAAVSIAEFRTGI
                     ELADTPERAARRARTLAAITSVIDVLDYTETTAAKHGRLLAYVRKPGTPRNAHDLIIA
                     AHAAETGRLVLTRDAKARFGDLPGVNAINA"
     gene            1948..>2559
                     /locus_tag="VF995_05355"
     CDS             1948..>2559
                     /locus_tag="VF995_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEX9343025.1"
                     /translation="MRLAVLDVGSNTVHLVVVDGRPDGTFTQVGRERETLRLADSAFP
                     AMLLPDEAVERLSATVLRMRKVADDLHADALAGFATSAIREARNGAEALGQVREETGV
                     PIAVLAGAEEARLTYLAARRWTAFSGRRLLVIDIGGGSLEVAGGEGERPELVESLPLG
                     ATRLSRRFVRSDPARPDDLVALRTHALALLGPLADLVREREWDA"
BASE COUNT          350 a          917 c          871 g          421 t
ORIGIN      
        1 gcacgtcgcc cacaccgtcg agagcatcgt cctgaagcat gcctgacgcc aggctgctct
       61 cggtcatcct gatcgtctgt gtggcagtcc tgttcgacta ctccaacggc ttccacgacg
      121 ccgccaactc gatcgccacc gtggtctcga ccagggtgct caccccccgg atcgcggtga
      181 tctgggcggc cgcgttcaac tttctcgcct tcctggtctt ccacgtgctg ttccacacca
      241 gcgtggccaa caccgtcggc aagtcggtct ccccggcgat cgtcagcgag gcggtggtgt
      301 tcgccggtct ggctggcgcg gtcatctgga acttcaccac ctggtggttc gggctgccga
      361 cctcgtcgtc gcacgcgctg atcggcggct ttgtcggcgc cggtgtcgcc aaggcaggct
      421 ggcacgtggt gaacagcgcc agcctccgca agaccgtgct gttcatcgtg ctgtcaccgc
      481 tgttcgggct ggtgttcgcc ttcctgttga tgttcgtgct gatgtgggtc ttccaccggt
      541 cgtcgccgcg ccgggtcgac cgcctgttcc gccgcctcca gctcttctcg gcggcggcct
      601 tcagcctcgg ccacggtggc aacgacgcgc agaagaccat gggggtggtg tccgcgctgc
      661 tggtcggcgc cggctacctg cacctcaacg ccaacggcga cctgccgctg ccgttctggg
      721 tggtgctgct cgcctacgcg gcgatcgcgg cgggcaccgc caccggcggc tggcgcatcg
      781 tcaagaccct tggccagaag atcaccgcgc tcaagccgat cggcggcttc tccgccgaga
      841 ccgccgcggc catctccatc tacatcgcca ccttcctcgg catcccggtc tccaccaccc
      901 acaccatcac cggcgcgatc gtcggcgcgg gctccacccg ccgcctctcc gccgtccgct
      961 ggggcgtcgc cggccgcatc gtctgggcct ggatcatcac catccccgcc gccggcgccc
     1021 tcgccgcggt cgcctactgg ctggtcgccc agcaaaacca cctgatcccc ctgctggccc
     1081 tggccgccct gctggtcctc agcggcgccc tggtcgccgc ccgccgcact ccgcagccga
     1141 gcgccgagcc cggaaccgga gcccgtgtga gcgctggcgc gggcagcgac tgaacggctc
     1201 gacaacaaat ctgattttca gatatgatgg ttggcatgag agtcatgaca gccaccgagg
     1261 cgtcccggca cttctcggac ctgctggacg cggtcgaacg tggcgagaca gtcaggatca
     1321 tgcgcggcag tcacccagtg gccgagatcg gccctgctcc ccgccgcaca ggtgcggacc
     1381 tcagcgcggc gctggcaggc gtcgaaccac tggatgagga tttcgccagc gacattgcgg
     1441 cagccttggc gctggtgaat tcggaggtgc ccgacccgtg ggccgacgcc tgatcctgga
     1501 tacgaacatg ctgatcgcct acgaacgcgg cagcttcgac cggtcgacgc tgcgcgacga
     1561 cgagcttgcc gtcgcggcgg tctccatcgc cgaattccga accgggatcg agctcgcgga
     1621 caccccggaa cgcgcggcca ggcgagcgcg gacactcgcc gcgatcacct cggtcatcga
     1681 cgtcctcgat tacaccgaga ccacagccgc gaagcacggc cggctgcttg cctatgtccg
     1741 taagccaggc accccacgca acgcgcacga cctcatcatc gccgcccacg ccgccgagac
     1801 cggacgcctt gtgctgaccc gcgatgccaa ggcccgtttc ggtgacctgc cgggcgtcaa
     1861 cgcgatcaac gcctgatcgg tagcggtaac gccgaaacgg ggcagagatt acggcttgag
     1921 gggcgcttgc ccgggtagga tctggggatg cggcttgctg tgcttgatgt cggctccaat
     1981 accgtccacc tggtggtggt ggacgggcgg cctgacggca ccttcacgca ggtcgggcgg
     2041 gagcgggaga cgctgcggct ggcggactcg gcgtttccgg cgatgctgct gccggacgag
     2101 gcggtcgagc ggctgagcgc caccgtcctg cggatgcgga aggtcgccga cgacctgcac
     2161 gccgatgcgc tggccgggtt cgccacctcg gcgatccggg aggcgcgcaa cggcgccgag
     2221 gcgctcgggc aggtgcgcga ggagaccggg gtgccgatcg cggtgctggc cggggccgag
     2281 gaggcgcggc tcacctacct ggccgcgcgg cgctggacgg ccttttccgg gcggcggctg
     2341 ctggtgatcg acatcggcgg cggcagcctg gaggtggccg ggggagaggg cgagcggccg
     2401 gagctggtcg agagcctgcc gctgggtgcc acccggttgt cgcggcggtt cgtccggtcg
     2461 gacccggccc gccccgacga cctggtggcg ttgcggaccc acgccctcgc gctgctcggg
     2521 ccgctggccg atctggtgcg cgaacgggag tgggacgcc
//
LOCUS       DASWDC010000170         9077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_939805, whole genome shotgun sequence.
ACCESSION   DASWDC010000170 DASWDC010000000
VERSION     DASWDC010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939805"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VF995_05360"
     CDS             <1..584
                     /locus_tag="VF995_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343026.1"
                     /translation="SFLCFSVGALVPLATYLLGFTSLWLALAFAARVPLFANNLGIWL
                     AGTAGGAMLLAGLLAGTFASVRGPGAGFLNGLTVWALLVVVGLAVGAPLLRLFNLTQV
                     GPLVRDNHIVRQSLWTAFIALLLSWAAATIGGTIGGAVPRDDGYELIEEEPEQSPLPT
                     RPDLAQDDLNGDVVNRDPLTSNPYRSYGGYSGP"
     gene            621..1769
                     /gene="cax"
                     /locus_tag="VF995_05365"
     CDS             621..1769
                     /gene="cax"
                     /locus_tag="VF995_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX9343027.1"
                     /translation="MTSAQTSARTAWSLDAFTRSERFVMLGALLLTVLLGVLQGLGAG
                     AVVRFAVAAVALALLAAVVGDAVEQLGARLTAGATGVLQSALGNLPELFFGYFALRQN
                     LVDVVRATLIGSILANNLLVLGLAFTVGGLRHGTQRFQREAPRLAVTLMMLAVSALIL
                     PTLAVSLHTPASTHIDGLSIACALVLLGVFIATIPSSLRAGPVGTPVTRAESAGSTGG
                     GAPAADQEAHRAWPLSHAVAVLLTASVAAALVSDWFVDVLTPATQTLHLSQTFTGLVI
                     VAIAGNAVENVVGVQFAARNQADQAVSVILNSPLQIALALGPILVLASFLLGTAHLTL
                     VLPPLLVVTVALATLLPTLIVFDGESTWLEGLALIGLYGVIAASFWWG"
     gene            complement(2014..2736)
                     /locus_tag="VF995_05370"
     CDS             complement(2014..2736)
                     /locus_tag="VF995_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HEX9343028.1"
                     /translation="MAAWAGPTAAAGAAPAIFHTPRIASLANTINASWASGNWSGYAK
                     TGAYHSATARWTVPTAARTARPTYSSSWVGIDGFNNSNLIQTGTEQDYFNGSAHYAAW
                     WEILPAAETVIPSITVHPGDVFTASVSQNSGTSWTIRITDTTTGATFSTTRTYTGPGT
                     SVEWIQEAPTVGGAIARLAHYGQATFDPGTANGANPALAATDGGVMIQNNRQVSTPSN
                     PDTDTDGFNAAYGATTPSPPGS"
     gene            complement(2866..3672)
                     /locus_tag="VF995_05375"
     CDS             complement(2866..3672)
                     /locus_tag="VF995_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX9343029.1"
                     /translation="MGAASARLRRLGAGGALDLIPSPGTGGGAARRVPVAGVVPDAAT
                     GAHELLVSRATAAALGQTTDRYLLLATDPGTAWQTVAARIRALLPAGTALRIRGPGQA
                     RYLRQADAVLAPVEEKTLSGEFAAKPTPAPGGWLTLDPRWVAAHITTASVPLLGPATC
                     NRAIFPQLRGALAEVQRRGLGGLVHRDDFAGCYAARLIPGDPGPSIAHHAWGTAIDLN
                     ARANPLGARPTQDPRLVAIFERWGFTWGGRFLVPDGMHFELLHLSMPVLP"
     gene            complement(3678..4181)
                     /locus_tag="VF995_05380"
     CDS             complement(3678..4181)
                     /locus_tag="VF995_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343030.1"
                     /translation="MEPETGTRHRHSPGTLLAVWLSLSLALAGCGAGTHRNHASPAAP
                     AAATAPRGAAPGAGAGAGTGAVTGAVTGAAAVVAPGTMLVWVSDGLPARFAARVSALP
                     GVRRVAAVVSGTVWLTRSFDASGRAVDKPPSGMAIPLELAGADPAALAPFVPAVAGSL
                     LAPLERG"
     gene            4486..5928
                     /locus_tag="VF995_05385"
     CDS             4486..5928
                     /locus_tag="VF995_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9343031.1"
                     /translation="MAGLALFGVLAARGGIAGLRHAPVAFWLFAVCLLVSELIPIVVP
                     GSQGPVEGFVMSDAFAFALLLGWGTAAAVLVGVAVSVAADRVHRRPLGALAFNAGQYG
                     IALAAAGGLYALADGHRPFHAGQFPAFVAAALVFIVLNEAFVRVALALDEHAPPHPRS
                     WSLLEGVRAQALPAAMLFGVAPVAVLLAERGAALLPLLVLPPVAVYLASRGTLQAETR
                     LRESERLKDDLLATVSHELRTPLASISALLNTLRQHGGRLEPAQREEFLGIAVTQSEQ
                     LRHMIEQMLLAARLRHDGGPAAPPTGPPVDAAALALRATQMARLAHPDRPIDLELELT
                     AEAGPGAVLPVRADQEAVLRILGNLLDNAVKYSPPRLPIRVEAQREREAAVLAVEDRG
                     PGIRPADRERAFERFTQLGPAHPGAPQTSGLGLGLHIARQLARANGGELVLADGAGGR
                     GARFELRLPLAEPDSAIQREAAAAPDPRRR"
     gene            6062..7300
                     /gene="lhgO"
                     /locus_tag="VF995_05390"
     CDS             6062..7300
                     /gene="lhgO"
                     /locus_tag="VF995_05390"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX9343032.1"
                     /translation="MTERYDLVVVGGGIIGLATARELRRRFPDASILVVEKERAWALH
                     QTGHNSGVIHSGIYYTPGSLKARLCAAGSQSMVAFCHERGVPVAVPGKLIVASEPREL
                     PRLEALLERGRANGLTLRRLDPAEIREREPHLRALAGIEVPTTGVVDYRQVAAALVHL
                     LQEEGVALRLGTEVRGFASGNGAKGREHLLHTHASDGDLRAGYLVTCAGLHADRLARA
                     AGADPGTRIMPFRGEYYELSPRARDLVRGLVYPVPDPAFPFLGVHLTRTLDGNVHAGP
                     NAVPAASREGYTWGSVSARDLAATFAYPGWWRLARRYWRTGMREITRSLSTRAFLADL
                     RRLYPEVSAADLLPAPAGVRAQALGRDGRLVDDFVIAHTDRATHVCNAPSPAATASLE
                     IARTIVAGLPDDAAGGRPSR"
     gene            7406..8149
                     /locus_tag="VF995_05395"
     CDS             7406..8149
                     /locus_tag="VF995_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9343033.1"
                     /translation="MVTGGGSGLGAATARELARRGARVAVIDRDEEAAAGVAKTLGEL
                     GRAFTADVTDEGQLKAAVDGAAAAFGGLRVAVCCAGVGVAQRVLGRDGPASLELFETV
                     LRVNLTGTYNTLRLAAAAMAGNDPDADGQRGVVVMTASVAAFDGQIGQTAYSASKGGV
                     AALTLPAARDLSRNGVRVCTIAPGIFDTPMLAGLPEAARESLAGGVPFPHRLGTPEEY
                     ALLACQIAEHPYLNGEVIRLDAALRMAPK"
     gene            complement(8109..8651)
                     /locus_tag="VF995_05400"
     CDS             complement(8109..8651)
                     /locus_tag="VF995_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004979298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX9343034.1"
                     /translation="MTAQTGPAAAILLATAALRRKLVAVTVRGTSMEPTYHDGDRVLV
                     LRGSALSPGQVVVAEQPATLTGGWHRQPVSSRTDARSRRWLIKRLAAVPGDPIPRHDA
                     PALARRQEDRVPPGMVVLLGDNRDMSLDSQAIGYFPVDRVLGVVLRALPRREPLPEPW
                     SPHHPPGPTSAPSAGRRPGG"
     gene            complement(8648..>9077)
                     /locus_tag="VF995_05405"
     CDS             complement(8648..>9077)
                     /locus_tag="VF995_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9343035.1"
                     /translation="MFLSEADRDDPETGVCLSGGQEQRLAIARAVLRDQCDLMILDEP
                     SAGLDAEAEHEIHTSLRCHRSGKTSLLISHRLGAVRDADTIAVLRDGQVTEHGDHPSL
                     MALGGAYARLFTLQASNYQIDAQQAEAETAGTTAFMGAVP"
BASE COUNT         1086 a         3355 c         3335 g         1301 t
ORIGIN      
        1 cgtcgttcct gtgcttctcg gtcggtgcgc tggtcccctt ggcgacctac ctgctcgggt
       61 tcacctccct gtggctcgcc ctcgccttcg ccgccagggt gccgctgttc gccaataacc
      121 tgggcatctg gctggcgggc accgccgggg gcgcgatgct gctggccggg ctgctggccg
      181 ggaccttcgc cagtgtccgg ggacccggcg ccggcttcct caacggcctg acggtgtggg
      241 cgctgctggt ggtcgtcggg ctggccgtgg gcgcgccgct gctgcggctg ttcaacctga
      301 cgcaggtcgg cccgctggtg cgcgacaacc acatcgtccg ccagtcactc tggaccgctt
      361 tcatcgcgct gctgctgagc tgggcggcgg ccaccatcgg cgggacgatc ggcggcgccg
      421 tgccccgcga cgacggctac gagctgatcg aggaggagcc cgagcagtca cccctgccca
      481 cccgcccgga cctcgctcag gacgacctca acggcgacgt cgtcaaccgc gacccactca
      541 cctccaaccc ctaccgcagc tacgggggct attcgggccc ctgagcccat cggccccgtc
      601 gcgccgttag tattggcgcc atgacgagcg cccagacttc cgcccgcacg gcctggtcgc
      661 tggacgcgtt cacccgtagc gagcggttcg tgatgctcgg cgcgctgctc ctgacggtgc
      721 tgctcggcgt cctgcagggg ctcggcgccg gggcggtggt ccgcttcgcg gtcgccgcgg
      781 tcgcgctggc gctgctggcc gccgtggtcg gcgacgcggt cgagcaactc ggcgcgcggc
      841 tcaccgccgg cgcgaccggc gtgctgcaat cggcgctcgg caacctgcca gagttgttct
      901 tcggctactt cgccctgcgc cagaacctgg tcgacgtggt ccgcgccacc ctgatcggct
      961 ccatcctcgc caacaacctg ctggttctcg gcctcgcctt cacggtcggc ggcctgcgcc
     1021 acggcaccca gcgcttccag cgggaggcgc cccggctggc ggtcaccctg atgatgctgg
     1081 cggtgtcggc gctgatcctg cccaccttgg cggtcagcct ccacaccccc gcgagcaccc
     1141 acatcgacgg cctcagcatc gcctgcgccc tcgtcctgct cggcgtgttc atcgccacca
     1201 tcccgtcctc gctgcgcgcc ggcccggttg gcactcccgt cacgcgcgcg gagtccgccg
     1261 gaagcacggg cggaggcgcg cccgccgccg atcaggaggc acaccgggca tggccgctgt
     1321 cgcacgccgt ggccgtcctg ctgaccgcca gcgtggcggc cgcgctggtg tccgactggt
     1381 tcgtggacgt cctcacgccg gccacccaga cgttgcacct ctcccagacc ttcaccggtc
     1441 tggtgatcgt cgcgatcgcc ggcaacgcgg tcgagaacgt ggtcggcgtc cagttcgccg
     1501 cccgcaacca agccgaccag gccgtcagcg tgatcctcaa cagccccctg cagatcgccc
     1561 tcgccctcgg cccgatcctg gtcctcgcga gcttcctgct cggcaccgcc cacctgacgt
     1621 tggtgctgcc gccgctgcta gttgtcacgg tcgcgttggc gacgctgctg ccgacgctga
     1681 tcgtcttcga cggcgagtcc acctggctgg agggcctggc gctgatcggc ctctacggcg
     1741 tcatcgcagc ttctttttgg tgggggtagt agggataccc cgggtgcgag actgcgccgc
     1801 ggcggggttg gggcggcagg gataccccgg gtgcgagcct gcgccttggc gtgggtggcc
     1861 cggcggggtc ccgttctccg ctccgaacag caggtcgcga gaacgggacc ccgccgggcc
     1921 tacggtggtc catgcggctc cggctcgcag ctcaccccta ggactcatcc ctaggaaagg
     1981 tgtcgagtgt ggtcagggct cgctgggggg cagttagctt cccggggggg agggggtggt
     2041 ggcgccgtag gcggcgttga agccgtcggt gtcggtgtcg gggttggagg gggtggagac
     2101 ctggcggttg ttctggatca tgacgccgcc gtcagtggcg gcgagggcgg ggttggcgcc
     2161 gttggcggtg cctgggtcga aggtggcctg gccgtagtgg gcgagacggg cgatggcgcc
     2221 gccgaccgtg ggggcctcct ggatccactc gaccgaggtg ccggggccgg tgtaggtccg
     2281 ggtggttgag aaggtcgcgc cggtggtggt gtcggtgatc cggatggtcc agctggtgcc
     2341 gctgttctgg gacaccgacg cggtgaacac gtcgcccggg tgcacggtga tggaggggat
     2401 caccgtctcg gcggcgggca ggatctccca ccaggcggcg tagtgcgccg acccgttgaa
     2461 gtagtcctgc tcggtgccgg tctggatcag gttgctgttg ttgaacccgt cgatgccgac
     2521 ccagctcgac gagtaggtcg gcctggcggt ccgggccgcc gtcggcacgg tccaccgggc
     2581 cgtggcgctg tggtaggcgc cggtcttggc gtagccggac cagttgccgg acgcccagct
     2641 ggcattgatc gtgttggcca gcgaggcgat cctcggcgta tggaagatcg ctggtgccgc
     2701 tcccgccgca gccgtaggtc ctgcccaggc tgccatcgag gcaagcagac cgactgccgc
     2761 gagacaccgc cgtatgcgca tattccacct cccgtgttat ccgcgttagg tgcgcattgc
     2821 cggtcaacta tggcaaacgt ttgcgaggtt gtcactttgt gcaggtcagg gcaatacggg
     2881 catgctcagg tggagcagct cgaagtgcat gccgtcggga accaggaagc ggccgcccca
     2941 ggtgaagccc cagcgctcga agatcgcgac caggcgcggg tcctgggtgg ggcgtgcgcc
     3001 gagggggttg gcgcgggcgt tgaggtcgat cgcggtaccc caggcgtggt gggcgatgct
     3061 cggtccgggg tcgccgggga tcagccgggc ggcgtagcag ccggcgaagt cgtcgcggtg
     3121 gaccagtccg ccgagcccgc ggcgctgcac ctcggcgagc gcgccgcgga gctgcgggaa
     3181 aatcgcgcgg ttgcaggtcg ccgggccgag cagcggcacg ctggcggtgg tgatgtgcgc
     3241 cgcgacccag cgcgggtcca gggtcagcca gccgccgggc gccggcgtgg gcttggcggc
     3301 gaactcgccg ctgagggtct tctcctcgac cggtgccagc accgcgtcag cctggcgcag
     3361 gtagcgggcc tgtccgggcc cgcggatccg cagcgcggtg ccggccggga gcagcgcccg
     3421 gatccgggcc gcgaccgtct gccaagctgt tcccgggtcg gtggcgagca gcaggtagcg
     3481 gtcggtcgtc tggccgagcg ccgccgcggt cgcgcgggag accagcagct cgtgcgcgcc
     3541 ggtcgccgcg tcgggcacca ccccggcgac cgggacccgc cgcgccgccc cgccgccggt
     3601 gcccggcgag gggatcaggt cgagggcgcc gcccgcaccg aggcgccgca gccgcgccga
     3661 ggccgccccc acacggccta cccgcgctcc agcggggcga gcaggctccc ggcgacggcc
     3721 ggcacgaacg gcgccagcgc cgccgggtcg gcgcccgcca gctccagcgg gatcgccatg
     3781 cccgacggcg gcttgtccac cgcccggccg ctggcgtcga acgagcgggt cagccacacc
     3841 gtcccgctca ccaccgccgc cacccgccgc accccgggaa gcgcgctgac ccgggccgcg
     3901 aaacgcgccg gcagcccgtc ggagacccac accagcatcg tccccggcgc caccacggcc
     3961 gccgcccctg tcacagcccc ggtcacagcc ccggtcccag ctccagcccc agctccggga
     4021 gcggcgcccc tgggcgcggt cgccgccgcc ggtgccgccg ggctggcatg gttgcggtgc
     4081 gtccccgcgc cgcacccggc gagggcgagg ctcaggctga gccagacggc gagcagggtt
     4141 ccaggggaat gacgatgccg ggtgccggtc tcaggttcca tagcgcagtg cggtggccgg
     4201 ggagtctgtt ccgcgtaaca gagcatctca tcccaaatgg cctacggcga atgcgcgacg
     4261 ctcggcacgg tcggtcccgc accgattccc gtaacggccg aagcgggagg aatggccggc
     4321 cagttgcttc cggttcggcg gtgcggcggt gaaaatgggc ctgagccggt acgtccggaa
     4381 ccggaaccag aaccagaagc cgctgcttcg agtgggtgca tggttcgtcc cagccgtccg
     4441 gcggcccgtc cgtcggcgtt gcgcctgtat tacgcggttg tgtccatggc cggcctggcc
     4501 ctgttcgggg tgctggcggc gagaggaggc atcgcggggc tccggcacgc gccggtggcg
     4561 ttctggctgt tcgccgtgtg cctgctggtg agcgagctga tccccatcgt ggtgcccggc
     4621 agccaggggc cggtcgaggg cttcgtgatg tcggacgcgt tcgccttcgc cctgctgctc
     4681 ggctggggca cggcggcggc ggtgctggtc ggcgtggccg tctcggtcgc cgccgaccgg
     4741 gtccaccgcc ggccgctcgg cgcgctcgcc ttcaacgccg gccagtacgg catcgccctt
     4801 gccgccgccg gcggcctcta cgccctcgcc gatgggcatc gccccttcca cgcggggcag
     4861 tttcccgcct tcgtcgcggc ggcgctggtg ttcatcgtcc tcaacgaggc gttcgtgcgg
     4921 gtggcgctgg ccttggacga gcatgccccg ccgcaccccc gatcctggtc attgctggag
     4981 ggcgtccggg cgcaggccct gccggccgcg atgctgttcg gggtggcgcc ggtcgcggtg
     5041 ctgctggcgg agcggggtgc cgcgctgctg ccgctgctgg tgctgccgcc agtggcggtc
     5101 tacctcgcga gcagaggaac cctccaggcg gagacgcgcc tgcgggagag cgagcggctc
     5161 aaggacgacc tgctcgccac cgtctcccat gagttgcgca cccccctggc cagcatcagc
     5221 gcgctgctca acaccttgcg ccagcacggc gggcgcctgg agccggcgca acgcgaggag
     5281 ttcctcggca tcgccgtcac ccagagcgag cagctccgcc acatgatcga gcagatgctg
     5341 ctggcggcac gcctgcgcca cgacggcggc cccgcggcgc cgcccacagg gccaccggtc
     5401 gacgccgccg cgctggcgct ccgggccacc cagatggccc gcctcgccca cccggaccgg
     5461 cccatcgacc tggagctgga gctgacagcg gaggccggcc ccggcgctgt gctaccggtg
     5521 cgggccgacc aggaggcggt cctgcggatc ctcggcaacc tgctcgacaa cgccgtcaag
     5581 tacagcccgc cacggctgcc gatccgggtg gaggcccagc gtgagcgcga ggccgccgtg
     5641 ctcgcggtgg aggaccgcgg ccctgggatc cggccggccg accgggagcg cgccttcgaa
     5701 cgcttcaccc agctcggccc ggcccatccc ggcgcgcccc agaccagcgg gctcggcctt
     5761 ggtctccaca tcgccaggca gctcgcccgc gccaacggcg gcgagctggt cctcgccgac
     5821 ggcgcgggag ggcgcggggc ccgcttcgag ctgcgcctcc cgctggccga gcccgactcc
     5881 gcgatccagc gggaggcggc cgcggcgcct gatccccggc ggcgctgatc cgctgcgacg
     5941 ggccgcgacg aggcagcccg cgagcgcccg cgagcgcccg cgagcgcccg cgggcgagca
     6001 tcggcgccga tctggcaagc tacgcgcgtg aacgacggtg gtggcggcct agagcggctc
     6061 gatgactgag cgctacgacc tggtggtcgt gggcggcggc atcatcgggc tggccaccgc
     6121 ccgggagctg cggcgacgct tccccgacgc ctccatcctg gtggtcgaga aggagcgtgc
     6181 ctgggcgctg caccagaccg ggcacaacag cggcgtgatc cactcgggca tctactacac
     6241 ccccgggagc ctcaaggcgc ggctctgcgc ggcgggcagc cagagcatgg tcgccttctg
     6301 ccacgagcgg ggcgtcccgg tcgccgtccc cggcaagctg atcgtggcga gcgagccgcg
     6361 ggagctgccc cggctggagg cgttgctgga gcgcgggcgc gccaacggcc tgacgctgcg
     6421 gcggctcgac ccggcggaga tccgcgagcg cgagccgcac ctgcgcgccc tcgccgggat
     6481 cgaggtgccc accaccgggg tggtcgacta ccggcaggtc gccgccgcgc tggtccacct
     6541 cctccaggag gagggcgtgg cgctccgcct cggcaccgag gtccgcgggt tcgccagcgg
     6601 caacggcgcc aagggccgcg agcacctcct gcacacgcat gcgagcgacg gcgacctgcg
     6661 cgccggctac ctggtgacct gcgcggggct gcacgccgac cggctcgccc gcgccgccgg
     6721 cgccgacccg ggcacgcgga tcatgccgtt ccgcggcgag tactacgagc tgtccccccg
     6781 ggccagggac ctggtccgcg ggctggtcta tccggtgccc gacccggcgt tcccgttcct
     6841 cggggtgcac ctgacccgca ccctggacgg caatgtccac gccggcccga acgcggtccc
     6901 ggccgccagc cgcgaggggt acacctgggg gtcggtgagc gcccgcgacc tcgccgccac
     6961 cttcgcctat cccggctggt ggcggctggc ccgccgctac tggcgcaccg gcatgcggga
     7021 gatcacccgc tcgctgtcga cgcgcgcctt cctcgccgac ctgcggcgcc tgtatcccga
     7081 ggtgagcgcc gccgacctgc tccccgcccc ggcgggcgtg cgcgcccagg cgctcggccg
     7141 cgacggccgc ctggtcgacg acttcgtgat cgcccacacc gaccgcgcca cccacgtctg
     7201 caacgccccc tccccagccg ccaccgcctc gctagagatc gcccgcacga tcgtcgccgg
     7261 ccttcccgac gacgcggctg gcggccggcc gtcccgttga ccgatccgtt cccgttccgg
     7321 gaagtccagt aaggaggcag ccgtgaccgc agcgtccagc gacggcccaa gggatccccg
     7381 cagagacctc cgtggcgctg gagcgctggt gaccggcggt ggctccggcc tcggcgcggc
     7441 caccgcccgg gagctggccc gccggggcgc ccgggtcgcg gtcatcgacc gggacgagga
     7501 ggccgcggcc ggggtcgcca agaccctggg ggagcttggc cgggcgttca ccgccgacgt
     7561 caccgacgag ggccagctca aggccgccgt cgacggcgcc gccgcggcgt tcggcggcct
     7621 ccgcgtcgcg gtctgctgcg ccggggtcgg ggtcgcccag cgggtcctcg gccgcgacgg
     7681 gcccgccagc ctggagctgt tcgagacggt gctgcgcgtc aacctgaccg gcacctacaa
     7741 caccctgcgc ctggccgccg ccgcgatggc tggcaacgac cccgacgccg acggccagcg
     7801 cggcgtggtg gtcatgaccg cctcggtcgc cgccttcgac ggccagatcg gccagaccgc
     7861 ctactccgcc tccaagggcg gcgtcgccgc gctcaccctg ccggccgccc gcgacctgtc
     7921 ccgcaacggc gtccgggtct gcacgatcgc cccggggatc ttcgacaccc cgatgctcgc
     7981 cggcctgccc gaggcggccc gcgagtcgct cgccggcggc gtccccttcc cgcaccgcct
     8041 cggcacgccc gaggagtatg ccctgctggc ctgccagatc gccgagcacc cctacctgaa
     8101 cggcgaggtc atccgcctgg acgccgccct gcggatggcg ccgaagtagg gcccggcggg
     8161 tggtgcgggc tccacggttc cgggagcggc tcgcggcgcg gaagggcccg gaggacgacg
     8221 ccgagcacgc ggtcgaccgg gaagtagccg atcgcctgcg agtcaaggct catgtcgcgg
     8281 ttgtcgccca gcagcacgac catgccgggc ggcacgcggt cttcctggcg gcgggcgagc
     8341 gcgggtgcgt cgtgccgtgg tatcgggtcg cctggtaccg ccgcgagccg tttgatcagc
     8401 caccgccgcg agcgcgcgtc ggtccgggat gacacgggtt ggcggtgcca gccgccggtg
     8461 agggtggcgg gctgctcggc gaccacgacc tgtccggggg agagggccga accacggagg
     8521 acgaggacgc ggtcgccgtc atggtaggtc ggttccatgc tggttccccg gaccgtcacc
     8581 gcgaccagct tgcgccgcag cgccgctgtg gccagcagta tcgccgcggc cggcccggtc
     8641 tgggccgtca cggtacggcg cccatgaacg cggtcgtccc ggccgtttca gcctcggcct
     8701 gctgggcgtc gatctggtag ttcgaggcct gcagggtgaa cagccgtgcg tacgccccgc
     8761 cgagcgccat caggctgggg tggtcgccgt gctcggtgac ctgaccgtcc cgcagcacgg
     8821 cgatcgtgtc ggcgtcgcgc accgcgccga ggcgatgcga gatcagcagg ctggtctttc
     8881 cgctgcgatg gcaccgcagg gacgtgtgga tctcgtgctc cgcctcggcg tcgagtcccg
     8941 cggaaggctc gtcgaggatc atgaggtcgc actggtcgcg gaggaccgcc cgggcgatcg
     9001 cgaggcgctg ctcctgcccg ccggagaggc acacccccgt ttcggggtcg tccctgtcag
     9061 cttctgagag aaacatc
//
LOCUS       DASWDC010000171         7145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1458462, whole genome shotgun sequence.
ACCESSION   DASWDC010000171 DASWDC010000000
VERSION     DASWDC010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458462"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VF995_05410"
     CDS             complement(<1..243)
                     /locus_tag="VF995_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX9343036.1"
                     /translation="MFIGTHDHSLDPKGRVILPRKFRDELGQDMVFTKGMGTERCLYV
                     FPVAEFEAFANKLRNLPLTGGRNRDFIRVFVANASQE"
     gene            complement(459..2048)
                     /locus_tag="VF995_05415"
     CDS             complement(459..2048)
                     /locus_tag="VF995_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9343037.1"
                     /translation="MRARRVLSSAQPLGRIARTLAPWLLPLLAGAIGALLAVTFLVHE
                     DARIGPVTVRLSARPALSGSSRLALPPFGSVSARTHLGPIAFHATLDDVDVPELGRMI
                     DNAPASGGLADALAPLERQADRAAAVFLLKLALLGLAGSLLGVIAFRRRSWRVLRRCL
                     LGGAIALQALLIPTVATFDVTAFRAPRYQGTVEYAPTLIGDVRTGVQRLHTLRDEMAL
                     ISENLNRAYDALAQPPPDLAGGGTVRVLHVSDLHLNPTGFDLAQRLAAYFEVDGVVDT
                     GDMGTWGLPIEPAIAAKVRGFQANGKQLPYLFVKGNHDSPDMVRAVAHNPNARVLNWS
                     ATTIRGISFYGVGDPSFSPGEGYRTDEFELLKRSKSIEVGEAIDARVPRPDVLLVHDP
                     QLATYAAGHVATVLDGHLHAFGLQLVNGTRMVTDGTTGAAGPDGFRNKNPQPYSAEVL
                     YFDTHSRRPLAVDRITVDSLRTDALQSTFSVQRILLPEGETPFVANPVQIPPELLQSP
                     ENALRQPPRQAPAVTVTTRQP"
     gene            complement(2057..2545)
                     /locus_tag="VF995_05420"
     CDS             complement(2057..2545)
                     /locus_tag="VF995_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX9343038.1"
                     /translation="MPVRRAVVLVLKEKADVLVPGAGLVRGASLALQAPSVIRLRYFV
                     KVPFRARAGLSRRAVFVRDDYLCQYCGVRAENVDHVIPRSRGGLHVWENVVAACRRCN
                     SHKEDRLPHEVGLRLRREPRAPKETLWIVIAVGRVEPGWAPFLGLDGLDGSQLEAGAV
                     PA"
     gene            complement(2723..4390)
                     /locus_tag="VF995_05425"
     CDS             complement(2723..4390)
                     /locus_tag="VF995_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9343039.1"
                     /translation="MTEQHPEAGMGFLEAVQETSQQLSGRAQGDDLLRQIVTSAAKVT
                     GIPHGYVCLREPEADEDELVLRVGIGAYECLVGLQIRKGEGLTGRVWDTAAPFTVDDY
                     ATWPDRVRVKNSPPLRSVLGVPLLSGGIVSGVIGLGSMDPERSFTGDEIAIIEQFAEL
                     AAIALETSRRYAASLRSETRYRTLVEMIPALVYSEDFKIGGSHMYANRYVETMFGFTQ
                     EEAAVPNSWKAQLHPDDRERVLAEEARCEQSGEPFRMEYRSFTRDGRVVWVRDECVLL
                     RDEDDTPLFWQGVLFDITESKLAEQRVKEALERERQAAQRLRSLDEMKNTFLDAVSHE
                     LRTPLAAVIGIALTLERAGDELPAADSADLIARLVANARKLDRLLTDLLDLDRLSRGI
                     MAPKRRPTDVAALVQRVAEEWCLLNGREVDLDTEPVSVALDAGKVERIVENLLANAAR
                     HTDADTQMWVRLRRESDGVLLAVEDAGPGIPPELRASVFEPFRRGPEVPSHAPGVGIG
                     LSLVARFAQLHGGRAWVDDRPGGGSSFRVFIPDAPARRASDRLGDDL"
     gene            complement(4504..5082)
                     /locus_tag="VF995_05430"
     CDS             complement(4504..5082)
                     /locus_tag="VF995_05430"
                     /inference="COORDINATES: protein motif:HMM:NF044444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9343040.1"
                     /translation="MPTGLETRAKLLVAARRAFAELGYAKARVEDVVSRAGVGHGTFY
                     AYFPNKRAALVALIQENAATLVDLASQPWRAGGVRASLMEVLGGLADLYDRDADVIAL
                     ATEASVQDPELGAVLDEVRRQFRNRIARNIERAQSQGVTRPVDPDTAAAALAAMAEQT
                     MYLRAVRKEPLDRDLTVRTLADLWFHALYLDA"
     gene            5289..6242
                     /locus_tag="VF995_05435"
     CDS             5289..6242
                     /locus_tag="VF995_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9343041.1"
                     /translation="MDALAESFGKIRGNIQRVIQGKPEAVRLTLIALLAEGHLLIEDV
                     PGVGKTMLAKALARSIDCSVRRIQFTPDLLPSDVTGVSVFNQERGDFEFKPGAIFANI
                     VLGDEINRASPKTQAALLESMEERQVTVDGTTYQLGVPFMVVATQNPIGHEGTYPLPE
                     AQRDRFMLELEIGYPTREAEIEILEGHGRVSKLSDIAPVADAPTVAQMIESCRNVHVA
                     DSLKRYIVDLVRATRDHADLALGASPRAALALLRAARAGAATHARDYVVPDDIKELVV
                     PVLAHRMIPAPEAQMAGRSAVDVLADIVEFVPIPIRGRGQA"
     gene            6255..>7145
                     /locus_tag="VF995_05440"
     CDS             6255..>7145
                     /locus_tag="VF995_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9343042.1"
                     /translation="MRRVPRLFTSRAAGFFAAALGLYLAGRLTGASELYMLAVACLAL
                     LGGSYLVVRWGNYRLACSRTVRPVRASMGGRIAVTVRLVNSSRLETGVLLLEDRLPYQ
                     LGTPARFVIPGIGGEDKQLLHYELPATTRGRYTIGPLAVRLVDPFGLAQAGSELAGTS
                     EVIVHPRVEALSPPALGGELASAAATRVRHLFSQGDEFYTTREYRDGDDLRKVHWRSS
                     AKRGQLMIRQEERPWQARALIALDLRHAAHRGQGGQASFERAVSIAASIALRLGQSGY
                     EIALVTDDGHQVRPPGGSDQA"
BASE COUNT         1047 a         2503 c         2566 g         1029 t
ORIGIN      
        1 ctcctggctg gcgttggcca cgaacacgcg gatgaagtcg cggttgcggc caccggtgag
       61 cggcagattg cgcagcttgt tcgcgaaggc ctcgaactcg gccaccggga acacgtagag
      121 gcagcgctct gtgcccatgc ctttcgtgaa gaccatgtcc tgtcccagct cgtcacggaa
      181 cttgcgcggc aggatcacgc ggcccttcgg gtcgagggaa tggtcgtggg tcccgatgaa
      241 cacagacccc tccctccctc agaccgtctg gcgcctttct cccccacttg actccacttt
      301 actccaccga ctatccaccg tcaacgcggt taacagttcg ttatacgaaa tagaaattgc
      361 gcctgagcag cggaaataca gctttgtatt ttcacgaatg ggacgaattc agcgaaccgc
      421 ttcgcctcca ccgcgctcca cttccgcagg tcaggaggct acggctggcg ggtggtgacc
      481 gtgaccgccg gcgcctggcg gggcggctgg cgcagtgcgt tctcagggct ttgcagcagc
      541 tccggcggga tctgcaccgg gttcgccacg aagggtgttt cgccttcggg gagcaggatg
      601 cgctggaccg agaaggtcga ctgcagcgcg tcggtgcgca gcgagtccac ggtgatccgg
      661 tcgaccgcca ggggccgccg gctgtgggtg tcgaagtaga gcacctcggc gctgtagggc
      721 tgcgggttct tgttgcggaa cccgtcgggg ccggcggcgc cggtggtgcc gtcggtgacc
      781 atgcgggtgc cgttgaccag ctggaggccg aaggcgtgca ggtggccgtc gaggacggtg
      841 gccacgtgcc cggccgcgta ggtggccagc tgcgggtcgt ggaccagcag cacgtccggg
      901 cgcgggacgc gggcgtcgat cgcctcgccg acctcgatgc tcttgctgcg cttgagcagc
      961 tcgaactcgt cggtgcggta gccctcgccg gggctgaagg aggggtcgcc gacgccgtag
     1021 aagctgatcc cgcggatggt ggtggcggac cagttgagga cccgtgcgtt ggggttgtgg
     1081 gcgaccgcgc ggaccatgtc ggggctgtcg tggttgcctt tcacgaacag gtacggcagc
     1141 tgcttcccgt tggcctggaa ccctcggacc ttggcggcga tggcgggttc gatcggcagg
     1201 ccccaggtgc ccatatcgcc ggtgtcgacc acgccgtcga cctcgaagta ggcggcgagc
     1261 cgctgggcca ggtcgaagcc ggtcgggttg aggtgcaggt cggagacgtg cagcacccgg
     1321 acggtgccgc cgccggcgag gtccggcggc ggctgggcga gcgcgtcgta ggcgcggttg
     1381 aggttctcgc tgatcagcgc catctcgtca cgcagggtgt ggaggcgctg caccccggtg
     1441 cgcacgtcac cgatcagggt gggcgcgtac tccaccgtgc cctggtagcg gggcgcgcgg
     1501 aaggccgtga cgtcgaaggt cgcgacggtc gggatcagca gcgcctgcag cgcgatcgcg
     1561 ccgccgagca ggcagcggcg cagcacccgc cagctccgcc gccggaacgc gatcaccccg
     1621 agcaggctgc cggcgagccc gagcagggcg agcttcagca ggaacacggc ggcggcgcgg
     1681 tccgcctggc gctccagcgg cgccagggcg tccgccaggc cgccgctggc cggggcgttg
     1741 tcgatcatcc ggccgagctc cgggacgtcc acgtcgtcga gggtggcgtg gaaggcgatc
     1801 ggaccgagat gggtgcgggc ggagaccgac ccgaacggcg gcaacgccag ccggctcgaa
     1861 ccggagaggg ccgggcgggc ggacagccgc acggtgaccg ggccgatgcg ggcgtcctca
     1921 tggaccagga aggtgaccgc cagcagcgcc ccgatcgccc cggcgagcag cggcagcagc
     1981 cacggcgcca gggtgcgggc gatgcggcca aggggctggg cggacgacag gacgcgccgc
     2041 gccctcaagc tgagcttcag gctgggacgg cgcccgcctc cagctgcgag ccatccaggc
     2101 cgtccaagcc gaggaagggc gcccagccag gctcgacgcg gccgactgcg atcacgatcc
     2161 acagcgtctc cttgggcgct cggggttcgc gacgcagacg gagtcccacc tcgtgcggga
     2221 gccggtcctc cttgtggctg ttacagcgcc ggcaggcggc caccacgttt tcccagacgt
     2281 ggaggccccc ccgtgagcgc ggtatgacat gatcgacgtt ctctgctcgt acgccacagt
     2341 actggcacag gtagtcgtca cgcacgaaca ccgccctgcg cgagagcccc gcgcgggcgc
     2401 ggaacggcac cttcacgaag taccgcaggc ggatcacgct gggcgcctgg agggccaggc
     2461 tggcgcccct gaccaggcca gcgccgggga ccaggacatc ggccttctcc ttgagcacca
     2521 acaccaccgc ccgccgcacc ggcacgacgc agatcggctg atatgacgca ttcaggacga
     2581 gggctcgctc caaaccgcgc tcccatcggc aaccacggca accacgggac gtgaccgcag
     2641 tataacgggg agcttcaagc acaatcaggc gtcctggaaa ccctgggcag ccgcagcccc
     2701 gagaggctcc ccgagaggct ccctagaggt cgtcgccgag ccggtcggag gcgcgccggg
     2761 cgggcgcgtc ggggatgaac acccggaacg aggagccgcc gccagggcga tcgtcgaccc
     2821 aggcccgtcc gccgtggagc tgggcgaagc gggccaccag cgacaggccg atgccgacgc
     2881 ccggggcgtg ggacgggacc tccgggccgc gccggaacgg ctcgaagacg ctggcgcgca
     2941 gctccggggg aattcccggg ccggcgtcct cgaccgccag cagcaccccg tcgctctcgc
     3001 gccgcaggcg cacccacatc tgggtgtcgg catcggtgtg gcgggcggcg ttggcgagca
     3061 ggttctcgac gatccgctcg accttgccgg cgtccagggc gacgctcacc ggctcggtgt
     3121 ccaggtccac ctcgcggccg ttcagcaggc accactcctc ggcgacccgc tggaccaggg
     3181 cggcgacgtc ggtggggcgg cgcttgggcg ccatgatccc gcggctcagc cggtccaggt
     3241 cgagcaggtc ggtgagcagc cggtcgagct tgcgggcgtt ggcgaccagc cgggcgatca
     3301 ggtcggcgga gtcggcggcg ggcagttcgt cgccggctcg ctccagggtg agggcgatgc
     3361 cgatcaccgc cgccagcggg gtgcgcagct catgggagac ggcgtccagg aaggtgttct
     3421 tcatctcgtc gagcgagcgc agccgctggg cggcctggcg ctcgcgctcc agcgcctcct
     3481 tgacgcgctg ctcggcgagc ttggactcgg tgatgtcgaa cagcacgccc tgccagaaca
     3541 gtggcgtgtc gtcctcgtcg cgcagcagca cgcactcgtc gcgcacccag accacccgcc
     3601 cgtcgcgggt gaagctgcgg tactccatcc ggaacggctc gccgctctgc tcgcagcggg
     3661 cctcctcggc cagcacccgc tcgcggtcgt cggggtgcag ctgggccttc caggagttgg
     3721 ggacggcggc ctcctcctgg gtgaagccga acatcgtctc cacatagcgg ttggcgtaca
     3781 tgtgggagcc gccgatcttg aagtcctcgc tgtagaccag cgccgggatc atctcgacca
     3841 gggtgcggta gcgggtctcg gagcggaggc tggcggcgta gcggcggctg gtctccaggg
     3901 cgatcgcggc gagctcggcg aactgctcga tgatcgcgat ctcgtcgccg gtgaagctcc
     3961 gctccggatc catcgagccg aggccgatca cgccggacac gatcccgccc gacagcagcg
     4021 gcacccccag caccgaccgc agcggcgggg agttcttgac ccggacgcgg tcgggccagg
     4081 tggcgtagtc gtccacggtg aagggcgcgg cggtgtccca gacccggccg gtcaggccct
     4141 cgcccttgcg gatctgcagg ccgaccaggc actcgtaggc gccgatgccg acccgcagga
     4201 ccagctcgtc ctcgtcggct tcgggttcac ggaggcagac gtagccgtgc gggattccgg
     4261 tcaccttggc ggcgctggtg acgatctggc ggagcaggtc gtcgccctgg gccctgccgg
     4321 agagctgctg ggaggtctcc tgcacggcct ccaggaagcc catgccggct tccggatgct
     4381 gctcagtcaa cctgtgcctc caccgcgatc acgtcctgtt cgcccgggga gcgccccttc
     4441 gagatttccg ggcagcactg aggggcatga atggggcatg cggttggaac ggtaccgcat
     4501 tgctcaagca tcgaggtaga gcgcgtgaaa ccacaggtcc gcgagcgtcc tgacggtcag
     4561 gtcgcggtca agcggctcct tgcgtaccgc gcgcaggtac atggtctgct cggccattgc
     4621 cgccagggcg gccgcggcgg tgtcggggtc gaccgggcgg gtgacaccct ggctctgggc
     4681 acgttcgatg ttgcgggcga tgcggttacg gaactggcgc cgtacctcgt ccaggaccgc
     4741 gccgagctcg gggtcctgga ccgacgcctc ggtcgccagc gcgatcacgt cggcgtcgcg
     4801 gtcgtagagg tcggcgaggc cgccgagcac ctccatgagg ctggcgcgga cgccgccggc
     4861 gcgccacggc tgggacgcca ggtcgaccag ggtcgcggcg ttctcctgga tcaaggcgac
     4921 cagcgccgca cgcttgttcg ggaaatatgc gtagaaggtc ccgtggccga ccccggcgcg
     4981 gctgaccacg tcctcgaccc ttgccttggc gtagcccagc tcggcgaacg cccgccgcgc
     5041 ggcgacgagc agcttggcgc gtgtctccag tcccgttggc acccaccaag cttatcgagc
     5101 cgatcccgga gccccaaagc cacccccgga accggtcgcc gcgctgcggt gtaccgagcg
     5161 gtgtatcccc gcgcgaacct accgattccg ccggaccaat ccgtaacttc gtggatacaa
     5221 tcgttgctca caggctgtcc aggaaaggag cccgcccggt ggccacaacc cggcctgaaa
     5281 cgggagggat ggacgctctc gccgaatcct tcgggaagat ccgcggcaat atccagcggg
     5341 tcatccaagg caagccggag gctgtccgcc tgaccctcat cgccctgctc gcggagggcc
     5401 acctgctgat cgaggacgtc cccggcgtcg gcaagaccat gctggccaag gcgcttgccc
     5461 gctcgattga ctgctcggtc cgccgcatcc agttcacccc cgacctgctg ccctcggacg
     5521 tgaccggcgt gagcgtcttc aaccaggagc ggggcgactt cgagttcaag ccgggcgcca
     5581 tcttcgccaa catcgtgctc ggcgacgaga tcaaccgggc ctcgcccaag acccaggcgg
     5641 ccctgctgga gtccatggag gagcgccagg tcaccgtcga cggcaccacc taccagctcg
     5701 gcgtcccgtt catggtggtg gcgacccaaa acccgatcgg gcatgagggc acctacccgc
     5761 tgccggaggc gcagcgcgac cgcttcatgc tggagctgga gatcggctac ccgacccgcg
     5821 aggccgagat cgagatcctg gagggccacg gccgggtctc caagctgagc gacatcgcgc
     5881 cggtcgccga cgcgcccacg gtcgctcaga tgatcgagag ctgccgcaac gtccacgtcg
     5941 ccgacagcct caagcgctac atcgtggacc tggtccgcgc cacccgcgac cacgccgacc
     6001 tggcgctggg cgcgagcccg cgcgcggcgc tggcgctgct gcgcgccgcc cgggcgggcg
     6061 ccgccaccca cgcccgcgac tacgtcgtgc ccgacgacat caaggagctg gtcgtccccg
     6121 tgctggccca ccggatgatc cccgcgccgg aggcgcagat ggccggccgc agcgccgtcg
     6181 acgtgctcgc cgacatcgtc gagttcgtcc cgatcccgat ccgtggccgc ggccaggcat
     6241 agggatccag gctgatgcgc agggtgccgc gcctgttcac ctcgcgggcg gccgggttct
     6301 tcgcggcggc gctggggctg tacctggccg ggcggctcac cggcgcctcc gagctgtaca
     6361 tgctggcggt cgcctgcctg gcgctgctcg gcggcagcta cctggtggtc cgctggggca
     6421 actaccggct cgcctgcagc cgcacggtgc ggccggtgcg ggcgtcgatg ggcggccgca
     6481 tcgcggtcac tgtccggctg gtcaactcca gccgcctgga gaccggcgtg ctgctgctgg
     6541 aggaccggct cccctaccag ctcggcaccc cggcccgctt cgtgatcccg gggatcggcg
     6601 gcgaggacaa gcagctgctc cactatgagc tgcccgccac cacccggggc cgctacacca
     6661 tcggcccact ggcggtgcgg ctggtcgacc cgttcggcct cgcccaggcc ggctcggagc
     6721 tggccggcac ctccgaggtg atcgtccacc cccgggtgga ggcgctgtcg ccgccagcgc
     6781 tcggcggcga gctggccagt gccgccgcga cgcgggtgcg gcacctgttc agccagggcg
     6841 acgagttcta caccacccgg gagtaccgcg acggcgacga cctgcgcaag gtccactggc
     6901 gttcctcggc caagcggggc cagctgatga tccggcagga ggagcggccc tggcaggcgc
     6961 gggcgctgat cgcgctggac ctgcgccacg ccgcccaccg cggccagggc ggccaggcct
     7021 ccttcgagcg ggcggtgtcg atcgccgcct cgatcgcgct gcggctcggc cagtccggct
     7081 acgagatcgc cctggtcacc gacgacggcc accaggtccg cccgccgggt ggctccgacc
     7141 aggcc
//
LOCUS       DASWDC010000172        10468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1043355, whole genome shotgun sequence.
ACCESSION   DASWDC010000172 DASWDC010000000
VERSION     DASWDC010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043355"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /gene="rfbD"
                     /locus_tag="VF995_05445"
     CDS             complement(<1..654)
                     /gene="rfbD"
                     /locus_tag="VF995_05445"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX9343043.1"
                     /translation="MRVLVTGAGGQVGSDLAAAFADAKELVALGHAELDVADRVAVAA
                     TVARVEPDLVLNAAAMTDVDGCERDQDRAFRDNCLGPRHLALACREAGAELLHLSTDY
                     VFDGQGATTRPDGYGEYDTPAPQSVYGRSKLAGEQALRDVWDRHYVVRTAWVYGRAGR
                     NFVKTILRLAAERDTLQVVADQVGSPTWSADLAAGLRALVGGGQHGTYHLTGGGHCSW
                     "
     gene            complement(661..1695)
                     /gene="rfbB"
                     /locus_tag="VF995_05450"
     CDS             complement(661..1695)
                     /gene="rfbB"
                     /locus_tag="VF995_05450"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX9343044.1"
                     /translation="MRFFVTGGAGFIGSNYVRHLLATDPDAEVTNFDKLTYAGNRDSV
                     ADLDADPRYRFVRGDICDGGLLAEALPGHDVVVNFAAETHVDRSIVEPLEAVRANTLG
                     VAALAEASRHAGVGRFLQVGTDEEYGPIVDGSFREHDRLEPSSPYSAGKAGGSLVALS
                     YATTHKLDVVVTRCTNNYGPCQFPEKVIPLFVTNLIDGHKVPLYGSGGNIRDWLYVLD
                     HCSAIDLVLRRGSSGQIYNVGSGNEVSNLELTRRILAAFGAGEELIEYVSDRPGHDWR
                     YSLDATRVRELGWTPAYDFDAGLAATIAWYREHEAWWRPLKPAGATRTLQVGRTSQPA
                     GPADPDPAGE"
     gene            complement(1798..3282)
                     /locus_tag="VF995_05455"
     CDS             complement(1798..3282)
                     /locus_tag="VF995_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX9343045.1"
                     /translation="MVTRKPSSPGADRWQNLEEIGRAARGTTAPPQPDPSPVRPDPAP
                     GGPGGPGGPGGPEWRPPSEPARRNRRNLLKWAAIALVVAVVAVSGVFGFALHTASTLL
                     GKSHQKVNGLVSAGGGPTNILLVGTDSREGLSQQDLNRVETIPVAGQRTDTIILIHVS
                     PDQKKAVMVSIPRDLKVAIPGHGTDKINAAYAFGGPDLLVKTVQQNLGVPVNHFASVD
                     FAGFLKVVDALGGVTLCNNTGHRLDDSFANLHMAPGCHNMNGIQALAFVRARHIDSDF
                     GRIGRQQQFLRAVMAKMTSKGNLIDVPKMLKVANIVSDHVKTDDTLKASEAISLARRL
                     HSLDSNSVDMRLYPSYDPGAACSTCADYVLASPEAPVLMNAIKNDAATLPPVGLPNGQ
                     GVSLRSVRVQVLNGSGMPGLAARAAQGLQAYGVRIAGTGNAPTQTGATSTLTYPARLA
                     RQAQLLGSLLGSQVKLAQSQSQSASDTLVLTVGSNFQVVAPPGQ"
     gene            3370..4293
                     /locus_tag="VF995_05460"
     CDS             3370..4293
                     /locus_tag="VF995_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03089 family protein"
                     /protein_id="HEX9343046.1"
                     /translation="METLPRTDLARLLDRQVARYQDKPLLTWYDDARGERVELSYKTF
                     ANWVAKTANLLVEELEVAPGERVALLLPTHWQTVVLRAACWLAGVRAGSSQEGVAAVF
                     AREERLGEVSAMATVPVVALTADWLGRPAGDLGGARNFARAAAAMGDHFEFEGAAEGD
                     GGPAAEAAAEAAALVELGEPLALASGLAGRLVLGERDRLHTSLPVEGPPGAVAALLAP
                     LLAGAGVVLEAAVDPRPGRFWRRLADERVTVACLDRAAVAALLEAPPSPAEAGAGGPR
                     PRVAWCPGRDAAELAERFERRFGVPLLTGVS"
     gene            4384..5820
                     /locus_tag="VF995_05465"
     CDS             4384..5820
                     /locus_tag="VF995_05465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9343047.1"
                     /translation="MKLSVVVPFYNELRTLPAVIDRLLAVDFAAMGLETELIFVDDGS
                     SDASRSLLDPAPRDDVRLIVHDHNQGKGAAVKTGLEAATGDILCIQDADLEYHPNDLP
                     ALIRPILEGAYQVVYGNRFRGSAAGLYYSHRIANRMLNLMINLAFNRYLEDVYTGYKV
                     FTKEAFSGLSLSAKTFTVEMELTAHFLRQGLVIYEVPISYRARTYAEGKKIHFKDGFL
                     AAGAVARYRFQRRPPTENPGPAMRRAPDVPGSPEIHALGLEDLAAARQYRRYIHDLLA
                     PYLGASVLEVEARLGDFSAELLDRRRLVLAESDPVCLRALEDRFEGHPRVELLEADPS
                     ALKVNPPVDSVVALNVIDLLPDDVAALRSMAQATAPGGTVLLLVPGFPSLAGAFDQAI
                     GEVRRYTPESLSATVTAAGLVPEVVRPVNLLGGVAWWAAVRMGRQGRPTPALVHLYDK
                     FVIPAERALERRVQPSFGQSILCVARVV"
     gene            complement(5831..6628)
                     /locus_tag="VF995_05470"
     CDS             complement(5831..6628)
                     /locus_tag="VF995_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9343048.1"
                     /translation="MQPRPRQAKVIVVMPAYNAARTLVRTYREIPGEVVDHVILVDDV
                     SRDDTVAVAELLQIQVVVHQQNRGYGGNQKTCYDTALAEGAEVVVMLHPDYQYDATRI
                     PALVAPIVSGEADMMLGSRFLGDPLAGGMPRWKYVANRFLTGLENKAFRLDLSEYHTG
                     FRAYSRSLLEQIDYHANSDDFVFDQELVSQVVAAGLRIGEVAVPTRYFAEASSVNFRR
                     SVVYGLGTLRTLGRHRRAVRRKRRADLPAVIKTKPTSTEQDTRTLKG"
     gene            complement(6730..8175)
                     /locus_tag="VF995_05475"
     CDS             complement(6730..8175)
                     /locus_tag="VF995_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343049.1"
                     /translation="MLLWLGVSGVTAALVRLPGPAVLDPDEHASSRYFQLLIHRRRLG
                     EFVYSAPKPLLTVVDGVAWTLTHDWRAITALTVAAFAVAVVTLARAAGRLVQGFGGPV
                     VAAAVTVALTSSGALILQVARGNSVVWALAGWGVAADALTRGRRRWGIAGVALLLASL
                     ARAESWLLLPLAAALAVLGWRRGERRALLLLLPLAAPLLWFGHDLALSGDALYSLRVP
                     ERYTDLISGRQVIPPGRWLVQLGRRYGRSPLLAALALAGLAWLLRQRAWLWLAGLGAM
                     TLGLLTVLGLDAWHGTYISFRYFDPADAGVRILAAFATAWVGPLARRARLPAGMVAHA
                     AAGGGAVALLVAACWPLAPADPAVRSTLDRGVALSRNAAAAIRVLRPVAAVPGTVVAV
                     SGPQRIRVAVELGLPLGRVRDLLVATQTEPLAQALSGSAAVFHDAAGDQPAARFAPLT
                     LTAPGRVGGLLAIPLLADPRRGVYVLRIAAG"
     gene            complement(8268..9728)
                     /locus_tag="VF995_05480"
     CDS             complement(8268..9728)
                     /locus_tag="VF995_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343050.1"
                     /translation="MGVAGGVLLAVAVLGLLVPFVRADPSARLTGSNAPFTDEGFNLA
                     NARARVLFGRFATGEIDRSLSNGAYSALAAALFAVTGPRLVAGRALSMVALAGAVLLL
                     AVGLAGPLGTRAALLAAVSLGGSDLALEYGRLALVEPLLVLLLCGALVLVARAVERPG
                     VAGGIGLGLLCAAAVSVKAIALVPVLALLSIAVGGALARRDRRSLSMALVACGTAVAA
                     AVVWLLAVALPNWGRLRTALRIWPRVHYPATPWGVVARLGHYLTHSDQALGRSWPLLA
                     ASLVGLVALVARWRSLPPATRGTLLAAALGGFGLWLAVALGDYAPNRYVVPALPGLAV
                     VAGFGLDAVVGVVAGRPGRRAAAALALGVALALLVAVPGAVTYLGGAFGSGQQRERDQ
                     RLLAARLPAGATVYGTYGPTLLFDTKVRVLTPWIPADANVDDPIRRFGVTDVLLGGRA
                     DPTGAVADFRDQRGMILLARVPWNGEQLSLYQVSGP"
     gene            complement(9749..10195)
                     /locus_tag="VF995_05485"
     CDS             complement(9749..10195)
                     /locus_tag="VF995_05485"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9343051.1"
                     /translation="MGVAQANLMGNVHGGEIMKLIDTAAGIAASRHAGGPAVTVSLDQ
                     MSFLSPVRVGNVVFVHASVNDVGRTSMEVGVRVEAEDFVTGERTHTSSAYLVFVALDE
                     RQRPRPVPPLEPETEVERRRQAEAKIRRESRLARAEAIRRGRGRDG"
     gene            complement(10275..>10468)
                     /locus_tag="VF995_05490"
     CDS             complement(10275..>10468)
                     /locus_tag="VF995_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005245270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9343052.1"
                     /translation="MKVTNDAVQLLGGYGYIRDFPAERMLRDARITAIYEGTNQIQRV
                     VVARHLLGGLTAEARASKR"
BASE COUNT         1485 a         3873 c         3736 g         1374 t
ORIGIN      
        1 ccaggagcag tggccgccgc cggtgaggtg gtaggtgccg tgctggccgc cgccgaccag
       61 cgcgcgcagg ccggcggcga ggtcagccga ccaggtgggg ctgccgacct ggtcggcgac
      121 cacctggagg gtgtcgcgtt cggcggcgag ccgcaggatc gtcttgacga agttgcggcc
      181 cgcccggccg tagacccagg cggtgcggac cacgtagtgg cggtcccaga cgtcgcggag
      241 ggcctgctcg ccggcgagct tggagcggcc gtagacgctc tggggcgcgg gggtgtcgta
      301 ctcgccgtag ccgtcggggc gggtcgtggc gccctgcccg tcgaagacgt agtcggtgga
      361 gagatgcagc agctccgcgc cggcctcgcg gcaggcgagc gccaggtgcc ggggccccag
      421 gcagttgtcg cggaaggccc ggtcctggtc gcgctcacag ccgtccacgt cggtcatcgc
      481 ggcggcgttg agcaccagat cgggttcgac ccgggcgacg gtggccgcga ccgcgacccg
      541 gtcagcgacg tccagctcgg cgtggccgag cgccaccagc tccttggcgt cggcgaacgc
      601 ggcggccagg tcgctgccga cctgcccgcc cgcgccggtg accagcaccc gcatggcggc
      661 ctactccccc gccgggtccg ggtcggccgg gcccgccggc tggctggtac ggccgacctg
      721 cagggtgcgg gtcgcgccgg ccggcttgag tggtcgccac caggcctcgt gctcgcggta
      781 ccaggcgatc gtggcggcca ggccggcgtc gaagtcgtag gcgggggtcc agccgagctc
      841 ccgcaccctg gtggcgtcca gggagtagcg ccagtcgtgg ccgggccggt cgctgacgta
      901 ctcgatcagc tcctcgccgg cgccgaacgc ggccaggatg cggcgggtca gctccaggtt
      961 gctgacctcg ttgccggagc cgacgttgta gatctggccg ctgctcccgc gccgcagcac
     1021 caagtcgatc gcgctgcagt ggtcgaggac gtagagccag tcgcggatgt tgccgccgct
     1081 gccgtagagc ggcaccttgt gcccgtcgat caggttggtg acgaacagcg ggatgacctt
     1141 ctcggggaac tggcagggcc cgtagttgtt ggtgcagcgg gtcaccacca cgtcgagctt
     1201 gtgggtggtg gcatacgaca gcgccaccag cgagccgccc gccttcccgg ccgagtacgg
     1261 cgacgacggc tccagccggt cgtgctcgcg aaacgacccg tcgacgatcg ggccgtactc
     1321 ctcgtcggtg ccgacctgca ggaagcggcc cacgcccgcg tgccgggagg cctcggccag
     1381 agccgcgacg ccgagggtgt tggcccgcac cgcctccagc ggctccacga tcgaacggtc
     1441 cacgtgcgtc tccgccgcga agttcaccac cacgtcgtgg ccgggcaggg cctcggcgag
     1501 caggccgccg tcgcagatgt cgccccgcac gaagcggtag cggggatcgg cgtcgaggtc
     1561 ggcgacgctg tcgcggttgc cggcgtaggt cagcttgtcg aagttggtga cctcggcatc
     1621 cgggtcggtg gcgagcaggt gccgcacgta gttggagccg atgaagccgg cgcctcccgt
     1681 cacgaaaaac cgcattccct ctcctcggcc tcgatcggcg actggaccag catgatggac
     1741 gccgagagcc taccaagccg ccagcacccg caccgggggc tccatggccc ggccggccta
     1801 ctgccccggc ggcgcgacca cctggaagtt cgagccgacc gtcaggacca gggtgtcgct
     1861 ggccgactgg ctctgggact gcgccagttt gacctggctg ccgagcagcg acccgagcag
     1921 ctgcgcctgc cgcgccagcc gcgcggggta ggtcagggtc gaggtcgcgc cggtctgggt
     1981 gggcgcgttg ccagtgccgg cgatccgcac gccgtacgcc tgcagcccct gggcggcccg
     2041 cgccgccagg cccggcatgc cgctgccgtt gagcacctgc acgcggacgc tccgcaggct
     2101 gaccccctgg ccgtttggca gcccgaccgg cgggagggtg gccgcgtcgt tcttgatcgc
     2161 gttcatcagc actggcgcct cgggcgacgc cagcacgtag tcggcacacg tcgagcaggc
     2221 cgcgcccggg tcgtagctcg ggtacaggcg catgtcgacc gaattgctgt ccagcgagtg
     2281 gaggcgccgc gccaggctga tcgcctcgct ggccttcagg gtgtcgtcgg tcttcacgtg
     2341 gtcggagacg atgttggcga ccttcagcat cttcggcacg tcgatcaggt tgcccttgga
     2401 ggtcatcttg gccatgaccg cgcggaggaa ctgctgctgg cggccgatcc ggccgaagtc
     2461 cgagtcgatg tggcgggccc gcacgaacgc cagcgcctgg atcccgttca tgttgtggca
     2521 gcccggcgcc atgtgcaggt tggcgaagct gtcgtccagc cggtggccgg tgttgttgca
     2581 cagcgtcacc ccgccgaggg cgtccaccac cttgaggaac ccggcgaagt ccaccgaggc
     2641 gaaatggttc accggcacgc ccaggttctg ctggacggtc ttgaccagca ggtctgggcc
     2701 gccgaaggcg taggccgcgt tgatcttgtc ggtgccgtgc ccggggatgg cgaccttcag
     2761 gtcccggggg atcgacacca tcacggcctt cttctggtcc gggctgacgt ggatcaggat
     2821 gatcgtgtcg gtccgctggc ccgcgaccgg gatggtctcc acccggttca ggtcctgctg
     2881 cgagagcccc tcccgggagt cggttccgac cagcaggatg ttggtcggcc cgccgccggc
     2941 cgagaccagc ccgttcacct tctgatggct cttgccgagc agcgtgctgg cggtgtggag
     3001 ggcgaacccg aagacgccgc tcaccgccac gaccgccacc accagcgcga tcgccgccca
     3061 cttgagcagg ttccggcggt tgcgccgggc cggctcgctc ggcggccgcc actccggccc
     3121 accggggcca ccggggccac cggggccacc gggggcgggg tcggggcgca ctgggctcgg
     3181 gtccggctgt ggcggcgcgg tggtcccgcg ggccgcacgg ccgatctcct cgaggttctg
     3241 ccagcggtcc gcgcccggcg aacttggctt tcttgtcacc atttctcgag cctagcatcg
     3301 gggtcttgac tgggtgcccc gggtggaccg aacgataacc ggccgcaacc gacccgttag
     3361 attgggcgca tggagacgct accaaggacc gatcttgccc gcctgctcga ccgccaggtc
     3421 gctcgatatc aggacaaacc actcttgacc tggtacgacg acgctcgcgg ggagcgggtg
     3481 gagttgtcct acaagacctt cgcgaactgg gtcgccaaga ccgccaacct gctggtcgag
     3541 gagctggagg tcgcgccggg cgagcgggtc gccctgctgt tgccgaccca ctggcagacg
     3601 gtggtgctgc gggccgcctg ctggctcgcg ggggtgcggg ccggaagctc gcaggagggg
     3661 gtcgccgccg tgttcgcccg cgaggagcgg ctcggcgagg tgtcggccat ggcgacggtg
     3721 ccggtggtgg cgctgaccgc cgactggctc ggccgccctg ccggcgacct gggtggggcg
     3781 cggaacttcg cgcgggccgc cgcggccatg ggcgaccact tcgagttcga gggcgcggcc
     3841 gagggcgacg gcggccccgc ggccgaggcc gcggcggagg ccgcggccct ggtggagctt
     3901 ggcgagccgc tcgcgctggc ctctgggctg gccggccgtc tcgtgctggg cgagcgtgac
     3961 cggctccaca ccagcctgcc ggtcgagggg ccgcccgggg cggtcgcggc gctgctggca
     4021 ccgctgctgg cgggcgcggg tgtcgtgctg gaggcggcgg tcgatccccg acccggcagg
     4081 ttctggcgcc gcctcgccga cgagcgggtg acggtggcct gcctcgaccg cgcggccgtc
     4141 gccgcgctgc tggaggcgcc gccgtccccg gccgaggcgg gcgccggcgg cccgcgcccg
     4201 cgggtggcgt ggtgccccgg ccgggacgcc gcggagctcg cggagcgctt cgagcgccgg
     4261 ttcggcgtcc cgctgctcac aggggtttca tgaggaggcc cgcaccctcc tgaacggcct
     4321 gccggctgcg gatgcgcgtg gtagtttgct caccctcgaa gcggacgcaa ggagagaagt
     4381 cgcatgaaac tgtcggtcgt cgtcccgttc tacaacgagc tccggacgct cccggcagtc
     4441 atcgaccggc tgctggcggt cgacttcgcc gccatggggc ttgagaccga gctgatcttc
     4501 gtggacgacg gctccagcga cgcgagccgc agcctgctcg acccggcgcc ccgcgacgac
     4561 gtgcgcctga tcgtccacga ccacaaccag ggcaagggcg cggcggtgaa gaccggcctg
     4621 gaggccgcca ccggcgacat cctctgcatc caggacgccg acctggagta ccaccccaac
     4681 gacctgccag cgctgatccg gccgatcctg gagggcgcct accaggtcgt ctacggcaac
     4741 cgtttccgcg gcagcgcggc gggcctgtac tacagccacc ggatcgccaa ccggatgctg
     4801 aacctgatga tcaacctggc cttcaaccgc tacctggagg acgtctacac cggctacaag
     4861 gtgttcacca aggaggcctt ctccgggctc agcctcagcg ccaagacctt caccgtcgag
     4921 atggagctga ccgcccactt cctgcgccag gggctggtca tctacgaggt gccgatctcc
     4981 taccgcgcac ggacctacgc cgagggcaag aagatccact tcaaggacgg cttcctggcc
     5041 gccggcgcgg tcgccaggta ccgcttccag cgccggccgc cgactgagaa cccgggtccc
     5101 gccatgcggc gggcgccgga cgtgccgggg tcgccggaga tccacgccct cgggctggag
     5161 gatctcgccg ccgcgcgcca gtaccggcgc tacatccacg acctgctcgc cccctacctg
     5221 ggcgcgtcgg tgctggaggt ggaggctcgg ctgggcgact tctccgcgga gctgctcgac
     5281 cgcaggcggc tggtcctcgc cgagtccgac ccggtctgcc tgcgtgccct ggaggaccgc
     5341 ttcgagggcc acccgcgggt cgagctgctg gaggccgacc cgtccgccct caaggtcaac
     5401 ccgccggtcg actcggtggt ggccctgaac gtgatcgacc tgctccccga cgacgtcgcg
     5461 gcgctgcgtt cgatggccca ggcgaccgcg ccgggcggca cggtgctgct gctggtcccc
     5521 gggttcccca gcctggccgg cgcgttcgac caggcgatcg gcgaggtgcg ccgctacacc
     5581 ccggagtcgc tcagcgccac ggtgaccgcc gccggcctgg tgcccgaggt cgtccgcccg
     5641 gtcaacctgc tcggcggggt cgcctggtgg gccgccgtgc ggatgggccg ccagggccgc
     5701 ccgacgcccg ccctggtaca tctctacgac aagttcgtga tccccgccga acgtgccctg
     5761 gaacgccgcg tgcagccgtc gttcggccag tcgatcctct gcgtcgcccg ggtcgtgtag
     5821 tcgaggggcc tcagcccttg agggtccggg tgtcctgttc ggtgctggtg ggcttggtct
     5881 tgatgacggc gggcaggtcg gcgcggcgct tgcggcggac cgcgcggcgg tggcggccga
     5941 gggtacgcag ggtgccgagg ccgtagacca ccgagcggcg gaagttcacc gagctggcct
     6001 cggcgaaata ccgggtgggg acggccacct cgccgatccg gaggccggcg gcgaccacct
     6061 ggctcaccag ctcctggtcg aacacgaagt cgtcggagtt ggcgtggtag tcgatctgct
     6121 ccagcagcga gcgggagtag gcacggaacc cggtgtggta ctcggacagg tcgagccgga
     6181 acgccttgtt ctccaggccg gtcaggaacc ggttggcgac gtacttccag cgtggcatcc
     6241 cgccagcgag cgggtcgccg aggaagcggc tgccgagcat catgtccgcc tcgccactga
     6301 cgatcggcgc gaccagcgcg gggatgcggg tggcgtcgta ctggtagtcg gggtggagca
     6361 tgaccaccac ctcggccccc tcggccaggg cggtgtcgta gcaggtcttc tggttgccgc
     6421 cgtagccgcg gttctgctgg tggaccacca cctggatctg cagcagctcg gcgaccgcga
     6481 cggtgtcgtc ccgcgacacg tcgtcgacca ggatcacgtg gtcgaccacc tcaccgggga
     6541 tctcccggta ggtccgcacc agggtccggg cggcgttgta cgcgggcatg acgacgatga
     6601 ccttggcctg gcgcggtcgt ggctgcattg actggctccg tcaggaacgg cgactcggaa
     6661 ggtggcgggc tggatgcatc gtaaatcaat cccgtcagcc ggggcgacca acctcggtgc
     6721 tcgcgccgct caccccgcag cgatccgcag gacgtagacg ccccggcggg ggtcggcgag
     6781 cagcgggatg gccagcaggc cgccgacgcg gccgggcgcg gtcagcgtca gcggcgcgaa
     6841 ccgtgcggcg ggctggtccc ccgcggcgtc gtgaaagacc gcggcgctgc cgctcagcgc
     6901 ctgcgcgagc ggctcggtct gggtggcgac cagcaggtca cggacccggc cgagcggcag
     6961 ccccagctcg accgctaccc ggatgcgctg cggcccggac accgcgacca ccgtacccgg
     7021 gacggcggcg accggtcgga gcacccggat cgcggcggcc gcgttgcggc tcaacgcgac
     7081 gccacgatcg agcgtcgacc ggaccgccgg gtccgccggg gcgagcggcc agcacgcggc
     7141 caccagcagc gccaccgccc cgcctccggc ggcggcgtgc gcgaccatgc ccgccggaag
     7201 ccgcgcccgc cgcgcgagcg gccccaccca ggcggtcgcg aaggccgcca ggatccgcac
     7261 gccggcgtcg gccgggtcga agtagcggaa ggagatgtag gtgccatgcc aggcgtccaa
     7321 ccccagcacg gtcagcaggc cgagggtcat ggcgccgagc ccggccagcc acagccacgc
     7381 ccgctgccgc agcagccagg cgagcccggc aagggccagg gcggcgagca acggcgagcg
     7441 tccgtagcgc cggccgagct gcacgagcca ccgcccgggc gggatgacct gccggccgct
     7501 gatcaggtcg gtgtagcgct ccgggacccg cagcgagtac agcgcgtcac cgctcagcgc
     7561 caggtcgtgc ccgaaccaca gcagcggcgc cgccaacggc aacagcagca gcagggcacg
     7621 ccgctccccc cgccgccagc cgagcacggc gagcgcggcg gccagtggca gcaggagcca
     7681 gctctccgcc ctcgccagcg acgccagcag caacgcgacc ccggcgatgc cccagcggcg
     7741 ccggccccgc gtgagggcgt cggccgcaac tccccaccca gccagcgccc agaccaccga
     7801 gttccccctg gcgacctgga ggatcagcgc ccccgaggag gtcagggcaa cggtgacggc
     7861 cgcggcgacg accggccccc cgaatccctg gacaagtcgc ccggccgccc tcgccagcgt
     7921 caccaccgcc accgcgaagg ccgccacggt cagggcggtg atggcacgcc agtcatgggt
     7981 cagcgtccac gcgaccccat cgaccacggt gagcagcggc ttcggcgccg agtacacgaa
     8041 ctccccgagg cgccgccggt ggatcagcag ctggaagtag cgcgaggacg cgtgttcgtc
     8101 aggatcgagg accgccggcc ccggaagccg taccagcgcg gcggtgaccc cgctcacgcc
     8161 cagccagagc aacacccacg gccaaggctg gccctcaggc ccggaggccg tgcgccctgt
     8221 cgacggactc ggcgaagccg agggtcgaga tgaggacgcc acccttttca cgggccagag
     8281 acctgataaa ggctgagctg ctcgccgttc cacggaaccc gtgcgagcag gatcatgcct
     8341 cgctggtcgc ggaagtccgc caccgcgccg gtcgggtcag cccgcccgcc gagcaggacg
     8401 tcggtgacgc cgaagcgccg gatcgggtcg tcgacgttgg cgtccgccgg aatccacggc
     8461 gtcagcaccc ggaccttcgt gtcgaacagc agcgtcgggc cgtaggtgcc gtacaccgtc
     8521 gctcccgccg gcaagcgagc ggcgagcagg cgctggtcgc gctcccgctg ctgcccactg
     8581 ccgaacgcgc cacccaggta ggtgacggct cccggcacgg cgaccagcag cgccagcgcg
     8641 acgccgagcg ccagcgccgc ggccgcccgc cggccaggcc gtcccgcgac cacgccgacg
     8701 accgcgtcca agccgaaccc ggccaccacc gcaagccccg gcaaggccgg gaccacatac
     8761 cggttcgggg catagtcccc tagcgcgacc gcgagccaga gcccgaagcc gccgagcgcg
     8821 gccgcgagca gcgtgcccct ggtcgccgga ggaagcgacc gccaacgcgc gaccagcgcc
     8881 accagtccca ccaaggacgc tgccagcaac ggccaggacc gcccgagggc ctggtcggag
     8941 tgggtcaggt agtgcccgag ccgggcaacg accccccacg gggtcgccgg gtagtggacc
     9001 cgcggccaga tccgcaacgc ggtccgcagc cgcccccagt tcggcagcgc caccgccagc
     9061 agccagacca ccgccgcggc caccgcggtg ccgcaggcga ccagtgccat ggagagcgaa
     9121 cgccggtcgc gccgcgccag cgcgccaccc acggcgatgc tgagcagcgc cagcaccggc
     9181 accagcgcga tcgccttgac tgacacggcc gccgcgcaga gcaaccccag cccgatcccg
     9241 ccggccacac cgggccgctc caccgcccgt gcgaccagca ccagcgcacc gcagagcagc
     9301 agcaccagca gcggctccac cagcgcgagg cgcccgtact ccagcgccag gtccgaccca
     9361 ccgaggctca cggcggcgag cagcgccgcc cgcgtgccga gcggcccggc aagcccgact
     9421 gccagcagca gcaccgcgcc ggccaacgcg accatggaga gcgcccgacc cgccaccagg
     9481 cgcggcccgg tgaccgcgaa cagggcggct gccagcgcgc tgtaggcccc gttggagagg
     9541 ctccggtcga tctcccccgt ggcgaagcgc ccgaacagca cccgcgccct ggcattcgcg
     9601 aggttgaacc cctcgtcggt gaagggcgcg ttgctcccgg tgagccgcgc cgacggatcg
     9661 gcccgcacga acggcaccag cagccccagc accgccaccg ccagcagcac cccgcccgcc
     9721 acccccacgc ggcgagcccg gctccggctc agccgtccct cccgcggccc cgccggatcg
     9781 cctcggcacg ggccaggcga gactcccggc ggatcttcgc ctcggcctgc cgccgccgct
     9841 ccacctcggt ctccggctcc agcggcggca ccggccgggg ccgctgccgc tcgtccagtg
     9901 cgacgaacac caggtacgcc gacgaggtat gggtccgctc cccggtcacg aagtcctcag
     9961 cctcgacccg gacgccgact tccatcgagg tccgcccgac gtcgttgacg ctggcatgca
    10021 cgaacaccac gttcccgacg cgcaccggcg aaagaaagct catctggtcc agcgacaccg
    10081 tcaccgccgg ccccccggca tgccggctcg cggcgatccc cgcggcggtg tcgatcagct
    10141 tcatgatctc cccgccgtgc acgttcccca tcaggttggc ctgcgccacc cccatcgcct
    10201 gcgacaacgt caccgccgac tcccgcggcg cctttggccc ttcatggctc ccggacagcg
    10261 tccctcctct ccctctaccg cttgctggct ctggcctcgg cggtcaggcc gccaagcaaa
    10321 tgacgtgcga caacgacccg ctgaatttga tttgtcccct cgtatatggc ggtaatgcga
    10381 gcgtcgcgga gcattctctc agcggggaag tcgcgaatat acccatagcc accaaggagc
    10441 tgtaccgcgt cgtttgtaac cttcatcg
//
LOCUS       DASWDC010000173         7737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_291551, whole genome shotgun sequence.
ACCESSION   DASWDC010000173 DASWDC010000000
VERSION     DASWDC010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291551"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VF995_05495"
     CDS             complement(<1..318)
                     /locus_tag="VF995_05495"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX9343053.1"
                     /translation="MHAWLLALAAAPQSCVPRAVYPELCDRGWLLGSLTLWDGCRDGG
                     VELMRVLAEVERSGFVESRHDGIVVVADADGEVLTAAGDPEEVVYARSCSKPFQALAV
                     YSLG"
     gene            439..1281
                     /locus_tag="VF995_05500"
     CDS             439..1281
                     /locus_tag="VF995_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343054.1"
                     /translation="MAPGPWLLYLEGISTRTTQVDLDDAGVLSVKVQTGACVEDLDLA
                     LGILRRAAEAGDGLVKSEEADTPFTLAEFEAAYGDEWKRRQLKSAADISIWMVRREGI
                     IALPGPTREVWIGPRVVEELENGDPAETGDRLVRVMRRVLWAEGPPFFAANRFRAGMG
                     AESVTLAVLPPETRTLLPRCDQVGLKDAGGTFMVAQKALPALPLGTTWLDDGNLLVEA
                     VSGEQWAAVCQAARALQGAEAAGGAGPPPRQPEPPSAPDATGPGPSLTRRLRRGWGRG
                     PRSS"
     gene            complement(1232..1990)
                     /locus_tag="VF995_05505"
     CDS             complement(1232..1990)
                     /locus_tag="VF995_05505"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX9343055.1"
                     /translation="MNPVTQQLDQLEATERQLLVFARDLNQLYHAERGRGAALDAACQ
                     DMVQTLALVVEAKDPSTRAHLQRAHDYAVALAGVVDPALADDRQLRFGFLLHDVGKIG
                     VPEAILTKAGPLDVDEWAVMRTHPELGERIVASISSLLGPAVAVIRSHHEHWDGGGYP
                     DGLRGEEIPLPARVFSVCDAFDAMTSDRPYRRALPLEVAFDQILQGSGTQFDPQMVDA
                     FGQLAPLLPALHASLHTGVTRTEDHAPSPYATCG"
     gene            complement(2152..2703)
                     /locus_tag="VF995_05510"
     CDS             complement(2152..2703)
                     /locus_tag="VF995_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HEX9343056.1"
                     /translation="MVRPAVIPGGQRRIDRVLDPAYSTDVAQLDLAELRRRRNEAEAE
                     EADISYLRRLLQGRLDILRTELVRRSQGADTDMSHLLAGLPGILSDEAPGTFNAIPRV
                     LLPGRATRHRRRVERLVSDETVAKLPELDIEALTEAVKVLAREEETVSANRRAIQRVV
                     DALRGELARRYRDGSANVGQLLE"
     gene            complement(2785..3657)
                     /locus_tag="VF995_05515"
     CDS             complement(2785..3657)
                     /locus_tag="VF995_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX9343057.1"
                     /translation="MCGAIAIERTDLAVVPLRGADTLRYLHLVCSQHTERLRPGDTSA
                     ALLLSAKGKIEFAFRIAVLDDGETVLLDTEAEAAPALAERLARFVFRYDVKVGDPQPG
                     VLTLLGPETAAASLMREAAPVAAHPLPGGLDLIGGDGTIPKVPEAPGVAVATAPLEAW
                     ERHRIAAGLPRWGRELTDDVIAEEAGLLGSHVHLDKGCYPGQETVARVHNLGQVQRRL
                     AGLRFKDPDAPPPAFRADLVSDDGRRAGQVRGAIHHPELGTIALAYVRRVIDPGRLVL
                     AGDQPATVVRLPFR"
     gene            complement(3941..4630)
                     /locus_tag="VF995_05520"
     CDS             complement(3941..4630)
                     /locus_tag="VF995_05520"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HEX9343058.1"
                     /translation="MRKLLIVLIVLGILGVAADFAAARVFESRVTTILEQRYDLGTRP
                     VIQVRDFPFLPHLITGDFSAIDLAARNVQAEGLSAEALELHLHGVHVPRKVMLGGSGD
                     VGVDRADGEVTLSEAEVNRLAASDLRGGTLSLEPDGVRLRVRVAILGQPVDALVAGQL
                     GVSAGRLTFTPKNVQAAGLPSAALGTVASLFTFEVPMRQLPAGLKLQRVTTERGALVV
                     AGQAASLQLPS"
     gene            complement(4714..4953)
                     /locus_tag="VF995_05525"
     CDS             complement(4714..4953)
                     /locus_tag="VF995_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX9343059.1"
                     /translation="MVRVRLFAALREAAGVAEVQVPAGQLGTVLEAVQGRFGDRFATV
                     LGWSSVLLDGQRCSDPSTPVPDGAELALLPPFSGG"
     gene            5050..5754
                     /locus_tag="VF995_05530"
     CDS             5050..5754
                     /locus_tag="VF995_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9343060.1"
                     /translation="MTNRGGGAKQVLPALEYLAHGVATLPLDLSSLAGVAGKDLVLID
                     ATRDLSRAQALCRTLRAAGEHSAILVVMPEAGLAGLQPDWGASDFVLPAAGPAEVEAR
                     VRLLVQRPETAADDSTVRVGELVVDPVSYQVRLHGRLLDLTYKEFELLRFLAARPGRV
                     STRAQLLQEVWGYDFFGGTRTVDVHVRRLRAKLGPEYESMIATIRGVGYKLLPADQAR
                     AEREAAGAGDSSAEPS"
     gene            5812..5884
                     /locus_tag="VF995_05535"
     tRNA            5812..5884
                     /locus_tag="VF995_05535"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5845..5847,aa:Lys,seq:ttt)
     gene            5902..5977
                     /locus_tag="VF995_05540"
     tRNA            5902..5977
                     /locus_tag="VF995_05540"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5936..5938,aa:Glu,seq:ttc)
     gene            complement(6056..7603)
                     /locus_tag="VF995_05545"
     CDS             complement(6056..7603)
                     /locus_tag="VF995_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEX9343061.1"
                     /translation="MTPTDTTYDVRVWTTQVNARSGGKTYTVRWRVATRRWKETFKTA
                     ALADSFRAGLLVAQRKGEAFDVATGRPVSLGRTTKQMTWYAFACAFADMKWPRVAATT
                     RRTHAEALTAVTTALLTTERGKPNGKLLRSALNRWGFNTARRNDENCPEEIRAALRWA
                     ADHTHPVSALRDPKVLRRVLDGLTVKLDGTPAAPSRTSRQKKILHAAVEYAVELELLT
                     ANPIPALKWKPPKTTPEVDRRRVVNPIQARTLLRTMGELPGGKRLVAFYACLYYAALR
                     PEEAVSLAKHNLSLPAEGWGDLHLERAEPFAGKDWTDSGRWRDDRHLKQRARGETRLA
                     PSPPELTALLHAHLAEFGTRPDGRLFRGERTATDLPARTVTRMWQRARRAAFTPEVAA
                     SQLAARPYDLRHAAVSTWLNGGVPPTVVAEWAGHSVEVLLKIYAKCLDRSDALIRARV
                     QAALGHPARPATHAKDAKDTMETGTSTAAPTAENPAANAPDEDSRTTTDTSAGTIRAQ
                     TPADGRI"
BASE COUNT         1092 a         2702 c         2820 g         1123 t
ORIGIN      
        1 cccaaggctg tacaccgcca gcgcctggaa cggcttggag cacgaccgcg cgtagaccac
       61 ctcctcgggg tctcccgccg cggtcagcac ctccccgtcg gcgtcggcca ccaccacgat
      121 cccgtcgtgc cggctctcca cgaacccgga acgctcgact tccgccagca cccgcatcaa
      181 ctcaactcct ccgtcacggc agccatccca gagtgtgagg ctacccagca gccagccacg
      241 gtcacacagc tctgggtaca cagctctggg tacacagctt tgcggagcgg cagccaaggc
      301 aagcagccac gcatgcatgt gatacttcac cgcatgagta caagctttca ggcacgaact
      361 gcccaacctg tcccgcgctc agtcatcgac ccaggcctcc cggaggttgc ctgggacagc
      421 gacgaaccct ccgaccccgt ggcgcccggg ccgtggctgc tgtatctcga aggcatctcg
      481 acccggacca cccaggttga cctggacgac gccggagtac tcagcgtcaa ggtacaaacg
      541 ggggcctgtg tcgaggacct cgacctcgcg ctgggcatcc tgcggcgcgc ggccgaggcc
      601 ggcgacggcc tcgtgaagag cgaggaggct gacacgccgt tcacgctggc cgagttcgag
      661 gcggcctacg gcgacgagtg gaagcgccgg cagctcaaat cggccgcgga catctccatc
      721 tggatggtgc gccgggaggg gatcatcgcg ctgcccggcc cgacccgcga ggtctggatc
      781 ggcccgcggg tggtcgagga gcttgagaac ggcgacccgg ccgagaccgg cgaccggctg
      841 gtccgggtga tgcggcgggt cctgtgggcc gaggggccgc ccttcttcgc cgccaaccgg
      901 ttccgggccg ggatgggcgc cgagtccgtc accctggccg tgctgccgcc agagacccgg
      961 accctgctcc cgcgttgcga ccaggtcggg ctcaaggacg cgggcgggac cttcatggtc
     1021 gcccagaagg ccctgccggc gctgccgctt gggacgacct ggctcgacga cggcaacctc
     1081 ctggtcgagg ccgtgagcgg cgagcagtgg gcggcggtgt gccaggcggc gagagcgctc
     1141 cagggcgccg aggcggctgg cggtgctggc cctccacccc ggcagcctga gccgccgtca
     1201 gcgccggacg cgacgggacc gggaccgtcc ctcacccgca ggttgcgtag gggctggggg
     1261 cgtggtcctc ggtcctcgtg acgccggtgt ggaggctggc gtgcagtgcc ggcagcagcg
     1321 gagcgagctg gccgaaggcg tccaccatct gggggtcgaa ctgggtgccg gagccctgca
     1381 ggatctggtc gaaggcgacc tccagcggca gggcgcggcg gtaggggcgg tcggaggtca
     1441 tcgcgtcgaa ggcgtcgcag accgagaaga cccgcgcggg cagggggatc tcctcgccgc
     1501 ggaggccgtc ggggtagccg ccgccgtccc agtgctcgtg gtgggagcgg atcaccgcca
     1561 cggccgggcc gagcagcgag ctgatgctgg cgacgatgcg ctcgccgagc tccgggtggg
     1621 tgcgcatcac cgcccactcg tccacgtcga gcgggccagc cttggtgagg atcgcctcgg
     1681 gcacgccgat cttgccgacg tcgtggagca ggaagccgaa gcgcagctgc cggtcgtccg
     1741 ccagcgccgg gtcgaccacc ccggcgaggg cgaccgcgta gtcgtgggcg cgctgcagat
     1801 gcgccctggt gctgggatct ttggcctcga ccaccagggc gagggtctgg accatgtcct
     1861 ggcaggccgc gtcgagcgcg gcaccccggc cacgctcagc atggtaaagc tggttgaggt
     1921 cgcgggcgaa cacgagcagc tgccgctcgg tcgcctcaag ctggtccaac tgctgcgtga
     1981 ccgggttcat ggcagggctc accatctggg acgtgccgct gcgcggcggc gctgactgga
     2041 cacgacgcgg ggtggcggct tctccactcc cccgctgcgt accaatcggt gcttcccgcg
     2101 cttgacttga gccctgaggt gtgatcgggg cttttgggag ctgcgccccg gtcactccag
     2161 cagctggccg acgttggcgc tgccgtcccg gtagcggcgg gccaactcgc cgcgaagagc
     2221 gtcgaccacg cgctggatcg cgcggcggtt ggcggagacc gtctcttcct cgcgggcgag
     2281 gaccttgacc gcctcggtca gcgcttcgat gtccagctcg ggcagcttgg cgacggtctc
     2341 gtcggagacc aggcgctcga cccggcggcg gtggcgggtc gcccggccgg gcagcaggac
     2401 gcgcgggatc gcgttgaagg tccccggcgc ctcgtcgctc aggatgccgg gcagcccggc
     2461 gagcagatgc gacatgtcgg tatcggcccc ctgcgagcgg cgcaccagct cggtccggag
     2521 gatgtccagc cgcccctgca gcagccgccg caggtaggag atgtccgcct cctcggcctc
     2581 tgcctcgttc cggcggcggc gcagctcagc caggtcaagc tgcgcgacgt cggtgctgta
     2641 ggccgggtcg agcacccggt cgatccggcg ctgcccaccg ggaatcaccg ccggacgcac
     2701 cacggcgcca tcctcggtcc cgcgtggttt catgaacatc cccccgaaat tacctgaagc
     2761 gcggattcta cctgaatggc aacctcacct gaatggcaac cttacgacgg tggcgggctg
     2821 gtcgccggcc agcaccagcc ggcccgggtc gatcacccgg cggacgtagg cgagcgcgat
     2881 cgtgcccagc tcgggatggt ggatcgcgcc gcggacctgg ccggcacgcc tcccgtcgtc
     2941 actgaccagg tcggcgcgga aggctggtgg cggagcgtcc gggtccttga agcggaggcc
     3001 ggcgaggcga cgctgcacct ggccgaggtt gtggaccctg gcgaccgtct cctggcccgg
     3061 atagcagccc ttgtcgaggt ggacatggga gcccagcagg ccggcctcct cggcgatgac
     3121 gtcgtcggtc agctcgcggc cccagcgcgg cagtcccgcc gcgatccggt gccgctccca
     3181 cgcctccagc ggcgccgtgg ccaccgcgac gccgggggcc tccggcacct tgggaatggt
     3241 cccgtcgccg ccgatcaggt cgaggccgcc cgggagcggg tgcgcggcca ccggggcggc
     3301 ctccctcatc aggctggccg cggccgtctc cgggccgagc agcgtcagca cccccggctg
     3361 cgggtcgccc accttgacgt cgtagcggaa gacgaagcgc gccagccgct cggccagggc
     3421 gggcgccgcc tccgcctcgg tgtccagcag gacggtctcg ccgtcgtcca ggacggcgat
     3481 ccggaaggcg aattcgatct tgcccttggc ggacagcagc agggccgcgc tggtgtcgcc
     3541 ggggcgcagc cgctcggtat gctgggagca caccaggtgc aggtagcgga gggtgtccgc
     3601 accccgcagc ggcaccacgg ccaggtcggt tcgctcgatc gcgatcgcgc cgcacatgca
     3661 cccatcctaa tcgcgttgcg gtccaccccc tccatgtggc taactggcca tacgcgagaa
     3721 aggaggtggt cccactgcat aaaccggtat ggacctgcga ggtgactgcc cgatagggca
     3781 acccgctcct gtatcgcggg acggatctgc ccgcggaatc cacacagatc accgaaccca
     3841 gggggcctgg cctggtccaa ccaggaaggc tgccgccccg tacggacggc acttacaacc
     3901 ccctgggtct tcgcattccc gcccgctccc ctggcccggc tcagctcggg agctggagcg
     3961 acgcggcctg gccggccacc accagggcgc cgcgctcggt ggtgacccgc tgcagcttca
     4021 ggccggcggg gagctgccgc atcggcacct cgaaggtgaa cagggaggcg accgtcccca
     4081 gcgcggcgct cggcagccca gcggcctgga cgttcttcgg ggtgaaggtg aggcggccgg
     4141 cgctgactcc gagctggccg gcgaccaggg cgtcgaccgg ctggccgagg atggcgacgc
     4201 ggacccgcag ccgcaccccg tccggctcaa gcgagagcgt gccgccgcgc aggtcgctgg
     4261 ctgccagccg gttcacctcc gcctcgctga gggtcacctc gccgtcggcc cggtcgacgc
     4321 cgacgtcgcc ggagccgccg agcatcacct tgcggggcac gtgcacgccg tggaggtgca
     4381 gctccagggc ttcggcgctg aggccctcgg cctggacgtt cctggcggcc agatcgatgg
     4441 cggagaagtc gccggtgatc aggtgcggca ggaaggggaa gtcgcgcacc tggatgaccg
     4501 gccgggtgcc caggtcgtag cgctgctcca ggatggtggt gacgcggctc tcgaagacgc
     4561 gcgcggccgc gaagtcggca gcgaccccga ggatcccgag cacgatcagg acgatcagca
     4621 gcttgcgcat ggcgagtgag aggttcccat ggaggcgggc ggcaaatcca agcagggcct
     4681 ttcgtaacaa accgcgcggt cacggggctg cgctcagccg cctgagaacg gcggcagcaa
     4741 tgccagctcg gcgccgtccg ggaccggcgt ggaggggtcg gagcagcgct gcccgtcaag
     4801 cagcacgctc gaccagccga gaaccgtggc gaaacggtca ccgaagcggc cctggaccgc
     4861 ctccaggacc gtgccgagct gcccggccgg aacctgcact tcggccaccc cggcagcctc
     4921 gcgcagggcg gcgaacaggc gaactcgtac catgaccgtg ccacggtatc gtgaggcgca
     4981 tctccacggc acacatctcc acggcacagg agacggaagg gaaagcacca ggtggccgag
     5041 cttgcctgct tgaccaaccg aggcgggggg gcgaagcagg tcctccccgc gctcgagtac
     5101 ctcgcgcacg gggtcgcgac cctgccgctc gatttgtcga gcctggcggg agtggccggc
     5161 aaggacctgg tcctgatcga cgccacccgc gacctgtcca gagcgcaggc gctgtgccgc
     5221 accctgcgag cggccggcga gcacagcgcc atcctggtgg tgatgcccga ggctggcctg
     5281 gccggcctgc agcccgactg gggcgcctcc gacttcgtgc tgcccgccgc cggccccgcc
     5341 gaggtcgagg cgcgcgtgcg cctgctggtg cagcgccccg agaccgccgc cgacgactcc
     5401 accgtccgcg tcggcgagct ggtggtcgac ccggtctcct accaggtgcg gctgcacggc
     5461 cgcctgctcg acctgaccta caaggagttc gagctgctgc gcttcctggc cgcccggccc
     5521 ggccgggtct ccacccgggc gcaactgctc caggaggtct ggggctacga cttcttcggc
     5581 ggcaccagga cagtcgacgt ccacgtccgc cgcctgcggg ccaagctcgg cccggagtac
     5641 gagtcgatga tcgccaccat ccggggcgtc ggctacaagc tcctccccgc cgaccaggcc
     5701 agggcggaac gcgaagccgc cggggccggc gacagtagcg cggaaccatc gtgatggaag
     5761 atcctttgac agtcagggca tgtcgatggc acgatcggcg cgctctgtcg tgggccgcta
     5821 gctcagttgg tagagcaccc ggcttttaac cggggtgtcc agggttcgat cccctggcgg
     5881 cccaccccaa agagacccgg ggcccccatc gtctagtggc ctaggacacc gccctttcaa
     5941 ggcggcgacg cggattcgaa ctccgctggg ggcaccacct tcgagccagc ctctatgtgc
     6001 gttgaccagc gcaaaccgtg gcgaggttgg tgaacatgtg ttcggctgtg tccggtcaga
     6061 tccggccatc ggcgggtgtt tgtgcccgta tcgtgcccgc gctcgtgtcc gtggtggtgc
     6121 gcgagtcctc gtccggggcg ttcgcggccg ggttctcggc ggtcggggca gcagtgctcg
     6181 tgcccgtttc catggtgtcc ttggcgtcct tggcgtgcgt ggcggggcgg gccgggtggc
     6241 cgagggcggc ctggacgcgg gcacggatga gggcgtcgct gcggtccagg cacttggcgt
     6301 agatcttcag cagcacctcg accgagtgcc cggcccactc cgcgaccacc gttggcggca
     6361 ctcccccatt cagccaggtg gacacggcgg cgtgccgcag gtcgtacggt cgggcggcca
     6421 actgggacgc ggcgacttcc ggggtgaagg ctgcccgccg cgcccgctgc cacatccgcg
     6481 tgaccgtcct ggcgggcagg tcggtggcgg tgcgctcacc ccggaagagg cggccgtcag
     6541 gtcgcgtccc gaactcggcg aggtgcgcgt gcagcaacgc cgtcagctct ggcggggagg
     6601 gcgcgaggcg ggtctcgcct cgcgcccgct gcttgaggtg ccggtcgtcg cgccagcggc
     6661 cggaatcggt ccagtccttg ccagcgaagg gctcggcacg ctcaaggtgc aggtcgcccc
     6721 agccctcggc gggcagcgac aggttgtgct tggcgaggct gacggcttcc tcgggccgca
     6781 gcgcggcgta gtacaggcag gcgtagaagg cgaccaggcg tttcccgcca ggcagttcgc
     6841 ccatggtgcg aagcagcgtg cgggcctgga tcgggttgac cacccggcgg cgatcgacct
     6901 cgggggtggt cttgggtggc ttccacttca gcgccgggat gggattggcg gtgagcagct
     6961 ccagctccac cgcgtactcg acggcggcgt gcaggatctt cttctgtcgg ctggtcctgc
     7021 tcggcgcggc cggggtgccg tcgagcttga ccgtcagccc gtccagcacc cgccggagca
     7081 ccttggggtc gcgcagcgcc gagacggggt gggtgtggtc ggcggcccag cgcagcgcgg
     7141 cacggatctc ctcggggcag ttctcgtcgt tgcggcgggc ggtgttgaag ccccagcggt
     7201 tgagggcgct gcggagcagc ttgccgttgg gcttgccgcg ctcggtggtc aacagcgccg
     7261 tcgtgacggc ggtgagggcc tcggcatggg tgcggcgggt ggtggccgcg acccggggcc
     7321 atttcatgtc ggcgaacgcg caggcgaacg cgtaccacgt catttgcttg gtggtgcggc
     7381 ccagggagac cgggcggccg gtcgccacgt cgaaggcttc acccttgcgt tgggcgacca
     7441 gcagcccggc gcggaagctg tcggccagcg ccgccgtctt gaaggtctcc ttccatcgtc
     7501 gggtggcgac ccgccaccgc acggtgtagg tcttcccgcc gctgcgcgcg ttcacctggg
     7561 ttgtccagac gcgcacgtcg taggtggtgt cggtgggggt cactggggaa ccggcctcac
     7621 cctccgcccg cagtgattga tggccacgag gcccgaagca cgtgcactaa cccgccgcgg
     7681 tcatagtcac gcgattgccg acgacgactc cgcgccagcg ccggggcggg ccaagtt
//
LOCUS       DASWDC010000174         9636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1459326, whole genome shotgun sequence.
ACCESSION   DASWDC010000174 DASWDC010000000
VERSION     DASWDC010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9636
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459326"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..2561
                     /locus_tag="VF995_05550"
     CDS             192..2561
                     /locus_tag="VF995_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HEX9343062.1"
                     /translation="MSESPIRRVVVLGAGTMGAAIAAHCANAGLRVDLLDIASPDPAS
                     EDRDAVVQAGFERMLKARPAALASPRLAAAIRLGNFDDNLDRLGEADWILEAIVEQLE
                     PKQDLAARVEKAAAAGAIISSNTSGIPLRTIAEGRGAEFRRRFLGTHFFNPPRYLKLV
                     ELIPTADTDPAVLARMRAFMEEVLGKGPVIAKDTPNFIGNRLGSFGKRDMRIALERGY
                     SIEEVDALAGPVMGRPKTAIFRLADQVGLDVMVGVAENLYELVPDDESREEFRSHELL
                     RRMLAAGRLGNKTGGGFYKRTQRDGQSVFDVLDLETFEYRPAKKPRLPVISAAREYDD
                     LGERLRFILDRADQGADRDAALIRDLLLPALAYAARRVPEISDTLEAVDHAMEWGYGH
                     EAGPFRTWDLLGVREAVRRMDDQGIEVAGWVRDFLAAGNQRFYQDGSVYSPVTGRYQP
                     VETDPRAIDLEAVKADGGRVAGNDSASLVDLGDGVLCLELHSPASAVDAGVIETAARA
                     LEELRQDRWTGLVLANQARNFCVGANLMEVGMAAAGGAYDQVGEAAATLQRLLAGFRD
                     ASKPVVAAPHGQTLGAGAEICMHSDRVVPTLETYIGLVEVGAGLIPAGGGCKELLRRV
                     VSPAMRADGATPLPYLQRVLETIGAAKVATSALEARELGFFTDADPIVMNPDHQLAAA
                     KRQVLALAANYRPPVDRSVYAAGRDARAALELGVRTLEWGRFASEHDALIGTTLARVL
                     CGGDLSQPQWVDEDHVLKLEREAFVALLHQQKTLERMQALLTSGKPLRN"
     gene            complement(2600..3772)
                     /locus_tag="VF995_05555"
     CDS             complement(2600..3772)
                     /locus_tag="VF995_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343063.1"
                     /translation="MKRRILVGSGVAGGVGLFLTGLKAANTALVPAARAADAAGSAVA
                     GGVALDSFAGSDDAKLASALSYIAQQTYRPTLLLANRLHQLSNTYDLFEGLSISGVGL
                     EREFARQTVVQARGSALFRVPAGQTNNMVKDVTIQNISFVGSASTSFLEPVELSSGPI
                     LAYSTIRACGFKLFSTVIQGRLLGVTLQDIYINQGYKTQLYLAGSDNFIGVGGHFFMD
                     SQNLSADQYLLRFAHMSRTDVGPLFITGTRATGIRVDNGYGITFSNTKSEAKAGDPSI
                     ATQGSAVVVTGGHGVVFRDCWFFNVMANPTATGTPSLHKGYITVTGGDGVLIAGCRFS
                     GGATFAPTNTPANTPCVYASGGSVLCRSNLAINGQALLYRQATAGLINADAAVTTG"
     gene            3965..5149
                     /locus_tag="VF995_05560"
     CDS             3965..5149
                     /locus_tag="VF995_05560"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX9343064.1"
                     /translation="MNEAVIVSGARTAVGRAPRGTLRATRPDDMAAAAIAEAVKRAGG
                     LDPAEVEDVLLGCAVPEGSQGLNVARVAALRAGLPEGVPAQTVNRFCSSGLQTIASAS
                     ERIMAGFASTVVAGGTESMSQVPTPTPTFSPNPDAVRTHPEYYMGMGLTGELVAERYG
                     ISRTDQDAFALRSHQLAAKAVDSGLFDPEIVPLPVAFDWVDEGGAARHRETVFARDEG
                     PRRDTSADALATLKPVFRRGGTITAGNSSQRSDGAAAVVVMERSRAERLGLQPLARFR
                     GFAVGGVAPEVMGIGPTVAVPKVLALTGLTLDDIDLIELNEAFASQSLAVIRELGLDP
                     DKVNVNGGAIALGHPMGATGAKLTVQLLAEMARRKARYGMVTMCVGGGMGAAGIFENL
                     QN"
     gene            5263..5742
                     /locus_tag="VF995_05565"
     CDS             5263..5742
                     /locus_tag="VF995_05565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9343065.1"
                     /translation="MASVAGRMAADDFVAMKTPPPSAWNSYDTLPEPAGQPALLEFGA
                     GPLSKLRGFVSRHAIAAGLDPARTVDLVLVVNEVASNSVLHGGGNGTLRIWQDDGQLV
                     CEVSDSGYFYERLAGSKRPPPDQEGGRGLWLANQLCDLVQVRSLPHGTVVRLHTRLR"
     gene            complement(5767..6921)
                     /locus_tag="VF995_05570"
     CDS             complement(5767..6921)
                     /locus_tag="VF995_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEX9343066.1"
                     /translation="MARPDSAVGSEAWTSFALDLYRRLAQGDGNLVVSPSSVAAVLAM
                     VLPGARGETAAEIAGLLHWTEAPERLAAAAATLDDAVLRRATEDGIALEVSNAVWIQE
                     GMRLRDEFAAALSERFRAALRRADFQHHAEAARQAINQLVAEQTHDKIQELFVRGALD
                     SRTRLALTNAIYLRARWRDPFDPAATRPEPFHLPGGGTVEAPMLRQRHRWRYAESEGD
                     GSGWQALELPYQGGGFAMVVLLPTAGGFEAFQRDLDLGRLATALSSLAGRDVRLTMPK
                     FRFEATLDLSGVLKALGMPAAFSAGADFSGITADEPLQVQTVVQKAYVEVDERGTTAA
                     AATGIAVRATAMAISGGPVEFRADRPFLFLVRHLESRLLLIAGRVADPTA"
     gene            complement(6924..7799)
                     /locus_tag="VF995_05575"
     CDS             complement(6924..7799)
                     /locus_tag="VF995_05575"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9343067.1"
                     /translation="MAIDEDARAYAFDLATAAQRVGDADGDATVAAGTWKTEVDPGWT
                     VVGRPNGGYLLALAARAGLAVAGQPDPLAASAHFLASPEPGPAELEVETLRAGRSVAT
                     VQVRLAQGGSLCLEALLSAGTLDPGAPVGWRAASGPPPLAPLEACVPGVADLPVPGGF
                     HVALLDHIDIRVDPACAGWLAGRPAGKLETRGWVRFHDGREPDPLALLAFADGFPPTS
                     FELGIPSWAPTLAMSVYVRDLPAPGWLRLFARGQQLQGGWFDEEVELWDTRDRLVAQS
                     RQLAGARTPDPRSRR"
     gene            complement(7902..8969)
                     /locus_tag="VF995_05580"
     CDS             complement(7902..8969)
                     /locus_tag="VF995_05580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX9343068.1"
                     /translation="MTTTSGWVDLTELEQRAQELLDPGVYGYFAGGADDELTLAANLA
                     AWGRLRLRPHVLRDVSAVSTATTLLGEPVPTPFLVAPMGYHRLAHPDGELATAAGAAA
                     AGVPLVASTVATTTLEDTAAAVPAAQRWFQLYVYRDRGYAAELVHRAAAAGYRALVLT
                     ADLATYGRRLREIRDQFKLPPWLEIANAAGRFTYDPDDPASISNQFDPGLTPEVIGWL
                     HELAGMPVVVKGVLRGDDAAACVDAGAIGVIVSNHGGRQLDTAVATAEALPEVVEAVG
                     DRAEVYVDGGIRRGTDVLKALALGARAVLVGRPVIWGLATGGAEGVQAVLDGLRAELE
                     RAMALCGAASVADLTPDLVVR"
     gene            complement(9023..>9636)
                     /locus_tag="VF995_05585"
     CDS             complement(9023..>9636)
                     /locus_tag="VF995_05585"
                     /inference="COORDINATES: protein motif:HMM:NF025454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HEX9343069.1"
                     /translation="PPPPPPPQAVPMPPPQPRYAAATPMPNAMANAMASPPPLASEKD
                     GGLQPAEVRHSFFQLMFESVARRRPGFRVPKFGYENWVGFAAGPFGFYDVAFTANGAV
                     RAGIYLDMQERTATKRLFDDLYSERMAIETAVGRILSWERLDDRRASRIVDYREVRNL
                     PDHSEQRVAAEWAAETVVVLMEALDTRLRTTAQILLSRVQGSV"
BASE COUNT         1400 a         3426 c         3505 g         1305 t
ORIGIN      
        1 ccaccttgat cggcatcgca gacctccatg tgcggtgccc atgccttcat acccagcctg
       61 ccagtcccaa ttgcacctat caacctattc cgcgaacctc tttaattacc aatccaattg
      121 acatcgacgt cggcgtcgaa attcaccttg cgctaccgtg tcggcggcga cacaccaatg
      181 gagggagccc tgtgagcgaa tcgcccatcc gccgggtcgt ggtgcttggc gcggggacca
      241 tgggagcggc catcgcggcg cactgcgcca acgccggcct gcgggttgac ctactcgaca
      301 tcgcctcgcc cgaccccgct tccgaggacc gcgacgcggt ggtccaggcc gggttcgagc
      361 gcatgctgaa ggctcgtccg gcggcgctcg ccagcccgcg gctcgccgcg gcgatccgcc
      421 tcgggaattt cgacgacaac ctcgaccgcc tcggcgaggc cgactggatc ctggaggcga
      481 tcgtcgagca gctggagccg aagcaggacc tcgcggcccg ggtcgagaag gccgccgcgg
      541 ccggcgcgat catcagctcc aacacctccg gaatcccgct gcggaccatc gccgagggcc
      601 gcggcgccga attccggcgg cgttttctcg gcacccattt cttcaacccg ccccgctacc
      661 tgaagctggt cgagctgatc ccgaccgccg acaccgaccc ggccgtgctg gcgcggatgc
      721 gcgccttcat ggaggaggtg ctcggcaagg gcccggtgat cgccaaggac acccccaact
      781 tcatcggcaa ccgcctcggc agcttcggca aacgcgatat gcgcatcgcg cttgagcggg
      841 gctactcgat cgaggaggtg gacgcgctcg ccgggccggt gatgggccgg ccgaagaccg
      901 ccatcttccg gctcgccgac caggtcggcc tcgacgtgat ggtgggcgtc gcggagaacc
      961 tctacgaact ggtccccgac gacgagagcc gggaggagtt ccgctcccac gagctgctgc
     1021 ggcggatgct cgcggcggga cgcctcggca acaagaccgg cggcggcttc tacaagcgca
     1081 cccagcgcga cggccagagc gtcttcgacg tgctcgacct ggagaccttc gagtaccgcc
     1141 cggcgaagaa gccgcggctg ccggtcatct ccgccgcccg cgaatacgac gacctgggcg
     1201 agcggctccg cttcatcctc gaccgcgccg accagggcgc cgaccgcgac gccgcgctga
     1261 tccgcgacct gctgctgccg gcgctcgcct acgccgcccg gcgggtgccg gagatctccg
     1321 acaccctgga ggcggtggac cacgccatgg agtggggcta cggccatgag gccggcccgt
     1381 tccgcacctg ggacctcctc ggcgtccgcg aggccgtccg gcggatggac gaccagggca
     1441 tcgaggtggc cggatgggtg cgcgacttcc tcgccgccgg caaccagcgc ttctaccagg
     1501 acggctccgt ctacagcccg gtcacggggc gctaccagcc ggtcgagacc gacccgcggg
     1561 cgatcgacct ggaggcggtc aaggccgacg gcggcagggt cgccggcaac gactcggcca
     1621 gcctggtcga cctcggcgac ggggtgctgt gcctggagct gcactccccc gccagcgcgg
     1681 tcgacgcggg ggtcatcgag acggccgccc gcgcgctcga agagctcagg caggaccgct
     1741 ggacaggctt ggtgctcgcc aaccaggcgc gcaacttctg cgtcggcgcc aacctcatgg
     1801 aagttggcat ggctgccgcg gggggcgcct acgaccaggt cggggaggcc gccgcgaccc
     1861 tccagcggct gctcgccggc ttccgcgacg cctccaagcc ggtggtggcg gcgccgcacg
     1921 ggcagacgct cggcgccggg gcggagatct gcatgcattc cgaccgggtg gtcccgacgc
     1981 ttgagaccta catcggcttg gtcgaggtcg gcgccggcct gatccccgcc ggtggcggct
     2041 gcaaggagct gctgcgccgc gtggtcagcc cggcgatgcg cgccgacggc gccaccccgc
     2101 tgccgtacct gcagcgggtg ctggagacga tcggcgccgc caaggtggcg accagcgcgc
     2161 tggaggcccg cgagctgggc ttcttcaccg acgccgaccc gatcgtgatg aaccccgacc
     2221 accagctcgc cgccgccaag cggcaggtgc tggcgctggc cgccaactac cggccgcccg
     2281 tggaccgctc ggtctacgcc gccgggcgcg acgcgcgggc cgcgctggag ctgggcgtcc
     2341 gcaccctgga gtggggccgt ttcgccagcg agcacgacgc gctgatcggc accacgcttg
     2401 cgcgggtgct ctgcggcggg gacctctccc agccccaatg ggtcgacgag gaccacgtcc
     2461 tcaagctgga acgggaggcg ttcgtggcgc tgctgcacca gcagaagacc ctggagcgga
     2521 tgcaggccct gctcaccagc ggcaaaccgc tgcggaactg atgcgccagc gccagctccg
     2581 cggcggccag ccgccgcgtt cagccggtcg tgacggcggc gtccgcgttg atcaggcccg
     2641 ctgttgcctg ccggtagagc agcgcctgcc cgttgatggc caggttcgac cggcacagca
     2701 ccgagccgcc ggacgcgtac acgcacggcg tgttggcggg cgtgttggtt ggcgcgaaag
     2761 tggcgcctcc ggagaacctg cagccagcta tcaggacgcc atccccgccg gtgacggtga
     2821 tgtagccctt gtggagcgac ggggtgccgg tggcggtggg attggccatg acgttgaaga
     2881 accagcagtc acggaagacc accccgtggc cgccggtgac aaccacggcc gagccctgcg
     2941 tcgcgatcga ggggtcaccg gccttggcct cgctcttggt attggagaag gtgatgccgt
     3001 agccgttgtc cacgcggatc ccggtggccc tcgtcccggt gatgaacagc ggtcccacat
     3061 ccgtcctgct catgtgcgcg aaccgcagca ggtactggtc ggcggagaga ttctgcgagt
     3121 ccatgaagaa gtggccgccc accccgatga agttgtcgct gcccgccagg tagagctggg
     3181 tcttgtagcc ctggttgatg tagatgtcct ggagcgtgac gccgagcagc cggccctgga
     3241 tgacggtgct gaacagcttg aagccgcagg ctcggatggt ggagtaggcc aggatcggcc
     3301 cggagctgag ctcaaccggc tccaggaagc tggtgctggc gcttcccacg aacgagatgt
     3361 tctggatggt gacgtccttc accatgttgt tggtctgacc ggcgggcacc cggaacaggg
     3421 cggaaccccg ggcctggacg accgtctgcc gcgcgaactc ccgctcaagg ccgacaccgc
     3481 tgatggacag gccctcgaac aggtcgtagg tgttgctgag ctggtgcagc cggttcgcca
     3541 gcagcagcgt gggacggtag gtctgctggg cgatgtacga cagggcgctg gcgagcttcg
     3601 cgtcgtcgct gcccgcgaac gagtcgagcg cgacaccgcc cgcgaccgcg ctcccggcgg
     3661 cgtcggcggc gcgggcggcc ggcaccagtg ccgtgttggc ggccttgagg ccggtgagga
     3721 acaagccgac gccaccggcc acgccggagc caaccaagat acgccgtttc acgcaaacct
     3781 ctccattctg cccagcgctt gtgggcggcc gatgctggga cactggggga aaggcgatcc
     3841 aagcacaaca gtaatctagt agacaaaaat gtcactaccg tacgatttac gtcaaaggtt
     3901 gcgcggctct caggttccac tcaccggcga gaccctgtga ccaagtggga atggagtggt
     3961 ggcgatgaac gaagctgtca tcgtgagcgg tgcccgcacg gccgtcgggc gggcaccccg
     4021 ggggacgctg cgggcgacca ggccggacga catggccgcc gccgcgatcg ccgaggcggt
     4081 caagcgggcc ggcggcctcg acccggccga ggtcgaggac gtgctgctcg gctgcgccgt
     4141 tcccgagggg tcgcagggcc tcaacgtcgc ccgggtcgcg gccctgcggg cggggctgcc
     4201 ggagggggtg cccgcccaga ccgtcaaccg cttctgctcg tcgggcctgc agacgatcgc
     4261 gagcgcgtcg gagcggatca tggccggctt cgccagcacc gtcgtggccg gcgggaccga
     4321 gtcgatgtcg caggtgccca ccccgacgcc gaccttctcc cccaaccccg acgccgtccg
     4381 gacccacccc gagtactaca tgggcatggg cctgaccggc gagctggtcg ccgaacgcta
     4441 cgggatcagc cgcaccgacc aggacgcctt cgcgctgcgc tcccaccagc tggccgccaa
     4501 ggcggtcgac tcgggcctgt tcgacccgga gatcgtgccg ctgccggtcg ccttcgactg
     4561 ggtcgacgag ggcggcgccg cgcggcaccg cgagaccgtg ttcgcccggg acgaggggcc
     4621 gcgccgcgac acctcggcgg acgcgctcgc cacgctcaag ccggtgttca ggcggggcgg
     4681 cacgatcacc gccggcaact cctcgcagcg cagcgacggc gccgcggcgg tggtggtgat
     4741 ggagcggagc cgcgccgaac ggctcggcct ccagccgctg gcgcgcttcc gcggcttcgc
     4801 ggtcggcggg gtcgcccccg aggtgatggg catcggcccg accgtggcgg tccccaaggt
     4861 gctggcgctg accgggctga cactcgacga catcgacctg atcgaattga acgaggcatt
     4921 cgcctcgcag agcctggcgg tgatccgcga gctgggcctg gacccggaca aggtgaacgt
     4981 caacggcggc gcgatcgcgc tcggccaccc gatgggggcg accggcgcca agctgaccgt
     5041 gcagctgctc gccgagatgg ccaggcgcaa ggcccgctac gggatggtga cgatgtgcgt
     5101 cggcgggggc atgggcgcgg ccggcatctt cgagaatctg cagaattgac cagactgctc
     5161 tggcatgaag tgtggccaaa ggggaaacca gaaacgctat gagcttgcgt gtgctgactc
     5221 ggcgggcgag tgccgagcca gcgggtaccg taggagcctg gcttggcgag cgtcgccgga
     5281 cgcatggctg ctgacgactt cgtcgcgatg aagacgccac cgccatcagc ctggaactcc
     5341 tatgacaccc ttccggagcc ggcggggcag cccgccctcc tcgaattcgg cgccggcccg
     5401 ctgagcaagc tgcgggggtt cgtgtcgcgt catgccattg ccgccggcct ggacccggcc
     5461 cgcacggttg acctggtgct ggtcgtcaac gaggtggcca gcaacagcgt cctccacggc
     5521 ggtggcaacg gaaccttacg gatctggcag gacgacggcc aactggtctg cgaggtctct
     5581 gactccggct atttctacga gcgtctggct ggcagcaagc gacctccccc cgaccaggaa
     5641 ggcggccgtg gcctgtggct cgccaaccag ctctgcgacc tggtccaggt gcgttcgctg
     5701 ccgcacggca ccgtcgtccg gcttcacacc cgtttgcgct gagcgccagc gcctcagcct
     5761 cgggcgctac gcggtcgggt cggcgacccg gccggcgatc agcagcagcc tgctctccag
     5821 gtgccggacg aggaacagga agggccggtc ggcgcggaac tcgaccgggc cgccgctgat
     5881 cgccatcgcg gtcgcgcgca cggcgatgcc ggtggcggca gccgcggtgg tgccgcgctc
     5941 gtcgacctcc acgtaggcct tctggacgac cgtttggacc tggagcggct cgtcggcggt
     6001 gatgccggag aagtccgccc cggcgctgaa ggcggccggc atgccgagcg ccttcagcac
     6061 gccgctcagg tcgagggtgg cctcgaagcg gaatttcggc atggtcagcc gcacgtcacg
     6121 gcccgcgagg ctcgacagcg ccgtggccag gcgtcccagg tccaggtcac gctggaatgc
     6181 ctcgaagccg ccggcggtgg gcagcaggac caccatggcg aagccgccgc cctggtaggg
     6241 cagctccagc gcctgccagc cgctgccgtc accctcgctc tccgcgtagc gccagcggtg
     6301 gcgctggcgc agcatcggcg cctcgacggt gccgccgccg ggcagatgga acggctccgg
     6361 ccgggtggcg gctggatcga acgggtcgcg ccagcgggcc cgcaggtaga tcgcgttcgt
     6421 gagggccagc cgggtgcgcg agtcgagcgc cccccgcacg aacagctcct ggatcttgtc
     6481 gtgggtctgc tcggcgacca gctggttgat cgcctgccgc gccgcttcgg cgtggtgctg
     6541 gaagtccgcg cgccgcagcg ccgcccggaa ccgctccgag agcgccgcgg cgaactcgtc
     6601 acggaggcgc atcccctcct ggatccagac cgcgttggag acctccagcg cgatgccgtc
     6661 ctcggtggcg cggcgcagga ccgcgtcgtc caacgtggcc gcggcggcgg cgaggcgctc
     6721 cggcgcctcg gtccagtgga ggaggccggc gatctcggcg gcggtctctc cgcgtgcccc
     6781 gggcaggacc atggccagca cggccgcgac gctggacggc gacaccacga ggttgccgtc
     6841 gccctgcgcg agccgccggt agaggtcgag cgcgaagctg gtccaggctt cgctgccgac
     6901 cgcgctgtcc ggcctggcca cccctaccgc ctcgaccgag gatcgggtgt gcgggcgccc
     6961 gcgagctggc gggactgggc gaccaggcgg tcgcgggtgt cccacagctc gacctcctcg
     7021 tcgaaccagc cgccctggag ctgctggccg cgggcgaaca ggcgcagcca ccccggcgcg
     7081 ggcaggtcgc ggacgtagac gctcatggcg agcgtcggcg cccagctcgg gatgcccagc
     7141 tcgaaggagg tcggcgggaa cccgtcggcg aaggcgagca gcgccagcgg gtcgggctcg
     7201 cggccgtcgt ggaagcgcac ccacccgcgc gtctccagct tcccggcggg gcggccggcg
     7261 agccatccgg cgcaggccgg gtcgacgcgg atgtcgatgt gatcgagcag ggcgacgtgg
     7321 aacccgcccg gcaccggcag gtccgccacg ccgggcacgc acgcctccag cggcgccagc
     7381 ggcggcggcc cgctcgcggc acgccagcca acgggcgcgc ccgggtccag ggtgcccgcg
     7441 ctcagcagcg cctccaggca caacgacccg ccctgcgcga gccgcacctg gacggtcgcg
     7501 accgacctgc cggcgcgcag cgtctcgacc tccagctcag ccggcccggg ttcgggcgag
     7561 gcgaggaagt gggcgctcgc cgccagcggg tcgggctgcc cggcgaccgc cagcccggcg
     7621 cgggccgcga gcgccagcag gtacccgccg ttggggcggc ccaccaccgt ccagccagga
     7681 tcgacctcgg tcttccaggt cccggcagcg acggtggcgt cgccgtcagc gtcgccgacg
     7741 cgctgggccg ccgttgccag gtcgaacgcg tatgcccggg catcctcgtc gattgccatg
     7801 ccgcctccac ccgtttgccc tgtccgctcc ggaggctgcc agcggtcggc tggccgcgca
     7861 agcccgcaag cggcaacggg gtgtcgcgag cacgagccgc ctcagcggac caccaggtct
     7921 ggggtgaggt cggcgacgct tgccgcgccg cagagcgcca tggcccgttc cagctcggcc
     7981 cggaggccat cgaggaccgc ctggacgccc tcggcgccgc cggtggcgag gccccagatg
     8041 acgggtcggc cgaccaggac cgcgcgggcg ccgagggcga gggccttgag cacgtcggtg
     8101 ccgcggcgga tgccgccgtc gacgtagacc tcggcgcggt cgccgaccgc ctcgaccacc
     8161 tcagggagcg cctcggcggt ggcgaccgcg gtgtcgagct ggcgcccgcc gtggttggag
     8221 acgatcaccc cgatggcgcc cgcgtccacg caggcagccg cgtcgtcgcc gcggaggacg
     8281 cccttgacca ccaccggcat ccccgcgagc tcgtgcagcc agccgatcac ctcgggggtg
     8341 agacccggat cgaactggtt ggagatgctg gccgggtcgt cggggtcgta ggtgaaccgc
     8401 ccggccgcgt tggcgatctc cagccagggc gggagcttga actggtcgcg gatctcgcgc
     8461 agccgcctgc cgtaggtggc gaggtcggcg gtgagcacca gcgcccggta gccggcggcg
     8521 gcggcgcggt ggaccagctc ggcggcatag ccacggtcac ggtagacgta gagctggaac
     8581 cagcgctgcg ccgctgggac cgcggcggcg gtgtcctcca gcgtggtcgt cgccaccgtg
     8641 gaggcgacca gcgggacgcc cgccgcggcc gcgccggcgg cggtggccag ctccccgtcg
     8701 ggatgggcga gccggtggta gcccatcggg gcgaccagga acggcgtcgg caccggctca
     8761 ccgagcagcg tggtggcggt ggagaccgcg ctgacgtcgc gcagcacgtg cggccgcagc
     8821 cgcaaccggc cccaggctgc caggttggcg gccagggtca gctcgtcgtc ggcgccaccg
     8881 gcgaagtagc catagacgcc cgggtcgagc agctcctgtg cgcgctgctc cagctcggtc
     8941 aggtccaccc acccagaagt cgttgtcatc gagaacgcaa gtctagccgc accgcgaggg
     9001 ccgagcgcag ctccccctcg acctagacgc tgccctggac ccgcgacagc aggatctgcg
     9061 cggtggtccg caggcgggtg tcgagcgcct ccatcagcac caccaccgtc tcggcggccc
     9121 attcggcggc gacgcgctgc tctgaatgat ccgggaggtt gcgcacctcg cgatagtcga
     9181 cgatccggga ggcgcggcgg tcgtcgagcc gctcccagga gaggatgcgc ccgaccgcgg
     9241 tctcgatcgc catgcgctcg gagtacaggt cgtcgaacag ccgcttggtg gcggtccgct
     9301 cctgcatgtc caggtagatc ccggcccgga cggcgccgtt ggcggtgaag gcgacgtcgt
     9361 agaacccgaa gggcccggcg gcgaagccga cccagttctc gtagccgaac ttcggcaccc
     9421 ggaagccggg gcggcggcgc gccaccgact cgaacatcag ctggaagaaa ctgtggcgca
     9481 cctcggccgg ctgcaggccg ccgtccttct ccgacgccag cggcggcggg ctcgccatcg
     9541 cgttcgccat cgcgttcggc atcggggtgg cggcggcata gcggggctgc ggcggcggca
     9601 tcggcaccgc ctggggcgga ggcggagggg gcggtg
//
LOCUS       DASWDC010000175         6129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_940752, whole genome shotgun sequence.
ACCESSION   DASWDC010000175 DASWDC010000000
VERSION     DASWDC010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940752"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VF995_05590"
     CDS             complement(<1..820)
                     /locus_tag="VF995_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9343070.1"
                     /translation="MVAAPELAGLLQQAAADYQQGSPKVNGSCVGVHVQSMEPAAAVD
                     KLAKGWPDSSAGTRPDVWVPPSSTWASLLALRLQAGNGAGGVVTDPQGSVASSPLVVA
                     MPRPMAQALGWPGKQPGWGDLLGLLRNPAGWGAAGHPEWGPVKLGQTTPNHSTPALQS
                     LLGAMLFAGSGGQPLTLGTLSKRSQDIGALLAGLGGLPGPHADLPSDFLAALQRADDG
                     GKALDFVSAAFLSEQQVWAYNHGNPGSDPATLGRHAPPKVPLAALYPSQGTLRSD"
     gene            complement(1362..2873)
                     /locus_tag="VF995_05595"
     CDS             complement(1362..2873)
                     /locus_tag="VF995_05595"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX9343071.1"
                     /translation="MGGPMLNVSLVDADAAGAPTDLLVVPVFGGLDPGPGAEAAAALG
                     VPLRSLLEARGFSGKLGEAVAVPALGRLAAATLLVVGLGDRGEADAEALRRAAGVIVR
                     HAAGAKQAVTTLVQALPGDPVGAVRAVAEGALLAAYRFDKYKQPRRDGGAASTDALRQ
                     LDLAVSGDDPDRQDLEGALAAGTVRAAATNLARDLSNEPANALHPADLAEAARAAVAG
                     KDVRVAVRDEEQLRREGFGGIVGVGQGAANPPRLIELSYQPEGARGHVVLVGKGITFD
                     SGGLSLKPPDGMITMKTDMSGAAAVLGVLSAVADLGVKVAVTGYLASAENMPSGSATR
                     PGDVLTMKNGKTVEVLNTDAEGRLVMADALALGAESKPDAMIDVATLTGACVVALGKR
                     ISGLLSNDDALAEELLAAASEAGERSWRLPLPDDYHKELDSDVADLKNVGERWGGAIT
                     AGLFLQEFVDGRPWAHLDIAGPARADAEDGYLAKGSTGAAVRTLLTWLERRSA"
     gene            2978..3298
                     /locus_tag="VF995_05600"
     CDS             2978..3298
                     /locus_tag="VF995_05600"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9343072.1"
                     /translation="MERRRLADLIAFDDARMAKHRLFETDRTLTDLYCLDVGQEQLLR
                     VHDDADKVILVMAGRVLARVDDDSAELTQYEILLIPAGAKHALKNLGPEPAVLVVFVG
                     PRLA"
     gene            3454..4332
                     /locus_tag="VF995_05605"
     CDS             3454..4332
                     /locus_tag="VF995_05605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9343073.1"
                     /translation="MHERQPSQTAMMAAAARAAHLVVDNEPYIFQDSVAMPLLGDLGE
                     EIVAYHRASGDHVVLSSTRAQVTARSRYTEDRLAELVLTDHVLTQYVILGAGLDTFAY
                     RAPLALATRLRVFEVDHPATQRWKRDLLAAAQLTPVAPDGLAFVGTDFETDPLTGRLT
                     AAGFDPAQPALVSWLGVTFYLTRDAIVATLGTLGSLAPGTELVVEYALPPELRDARGA
                     AYAEFALPAAAERGEPWLTFLTPEDMSTLLRTHGFEVVKHLRQHDAIDPALWQRSDAL
                     RPADVCRLVRAVVAPR"
     gene            complement(4352..5374)
                     /locus_tag="VF995_05610"
     CDS             complement(4352..5374)
                     /locus_tag="VF995_05610"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9343074.1"
                     /translation="MKRAVVPLVLAATLAVALAACGNSPARQAQAGARPGQTQVALSL
                     DWTPNPDHVGLYYAQSKGLFQQAGLTVRMRAPSDPSAPLKLVALRKADLAISYEPDLF
                     FAAAKHLPVTAVASVIPVPLNSLIALPSSGITTPASLKGRKVGITGIPSDDAILQTLE
                     RSAGLSHGDVTPVSVGYNLVPALLSHKVDAILGGYRNVEAIQIAQQTGRAPVTLPVDR
                     LGVPSYDELVLVANTTRLRGDPAYATTVRRFITAMLAGASAARADQPAAVTAMTRATT
                     YTPKFLQASVPTTLTLLTPPDRPIGCLDQSAWQRYGDWMHTTGLLPTAISATTVMTTA
                     YLPSRC"
     gene            complement(5371..>6129)
                     /locus_tag="VF995_05615"
     CDS             complement(5371..>6129)
                     /locus_tag="VF995_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9343075.1"
                     /translation="LALLLGGLVLWELLVRALRVPAYLLPAPSAVAADFHTDAGLLAS
                     ATWVTLREVVLGFLLACATGLALALALHLSATLRRALYPLLIASQTVPVVVLAPILVI
                     LLGFDLAPKLVIVALICFFPIVVNGTDGLRSVDPELVRMMRTLDASRWAILKRVEWPS
                     ALPMVFSGLRVAATYAAVGAVFGEWAGSNAGLGYVMLQATPNLDTARIFAAIVILTVL
                     SLALFLLVSLAERLAVPWARGGGNGNGSGGALHP"
BASE COUNT          885 a         2182 c         2216 g          846 t
ORIGIN      
        1 agtcgctccg cagcgtcccc tggctgggat agagcgcggc cagcggcacc ttgggcgggg
       61 catgcctgcc gagggtggcc gggtcgctgc ccggattgcc gtggttgtag gcccacacct
      121 gctgctcgct gaggaacgcc gccgagacga agtcgagcgc cttgccgccg tcgtcggcgc
      181 gctgcagcgc ggcgaggaag tcgcttggga ggtccgcgtg cgggccgggg agccccccga
      241 gcccggccag cagcgcgccg atgtcctggc tccgcttgct gagcgtgccg agcgtgaggg
      301 gctggccgcc cgacccggcg aagagcatcg ccccgagcag cgactgcagc gccggggtcg
      361 aatggttggg ggtggtctgc ccgagcttga ccgggcccca ctcgggatgc ccggccgcgc
      421 cccagccggc cgggttgcgc agcaggccga gcaggtcgcc ccagccgggc tgcttgcccg
      481 gccagccgag cgcctgcgcc atcggccgcg gcatcgccac caccagcggc gagctcgcga
      541 cgcttccctg cgggtcggtc accacgccgc cggcgccgtt gccagcctgc agccggagcg
      601 cgagcaggct cgcccaggtg gacgagggcg ggacccagac gtccggccgc gtcccggcgc
      661 tgctgtcggg ccagcccttg gcgagcttgt cgaccgcggc cgccggctcc atgctctgaa
      721 cgtggacgcc gacgcaggag ccgttcacct tgggcgaccc ctgctggtag tcggctgccg
      781 cctgctggag cagcccggcc agctcgggcg ccgccaccac ctcaagcggg atgcgcgtcc
      841 ctccgcagcc cccgccggcc gacccgcccc gctcccgttc gcgccgtact gccgcgccga
      901 cctggtgacg atcagcgtcc ccgcaatcgc cgccagcacc agggtgaggc tgaccaggac
      961 cacccagccg gtgacccggc gcggcgaccc accagggtcg gggtccgcga gatgcctgcc
     1021 ccgctgccgt gagctctcca cgttgcgcat acgtagtaag cctccagatc cccccaattc
     1081 gcggcccgaa ggtgtgcaga aaggtgtgcg gaagggaagc agcggcagat gcgccttgga
     1141 caacccgtcc cttgacatcc aaggtgctca aactatgacg ggtaatctat ttcaggacaa
     1201 aatagctacc caacgaaaca agtcacgtaa gcatacgtaa gcgctcaaca acgcatagag
     1261 gcgcagtcaa gccgcacggc gcgcttgaat ccctcggttc gattgcgaac ggttcaggag
     1321 cccggcgccg ggtcacggcg ccgggccggc aagcgcctgg ctcaggcgct gcggcgctcc
     1381 agccaggtca gcagggtgcg gaccgcggcg ccggtcgagc ctttggcgag gtacccgtcc
     1441 tccgcgtccg ctcgtgccgg gccggcgatg tccaggtggg cccagggccg cccgtcgacg
     1501 aattcctgca ggaacagccc ggcggtgatg gcgccgcccc agcgctcgcc gacgttcttc
     1561 aggtcggcga cgtcgctgtc cagctccttg tggtagtcgt cggggagcgg cagccgccag
     1621 ctccgctccc ccgcctcgct ggcggccgcc agcagctctt cggcgagcgc gtcgtcgttg
     1681 gagagcagcc cgctgatacg cttgccgagc gccaccacgc aggcgccggt gagggtggcc
     1741 acgtcgatca tcgcgtcggg tttcgattcc gcgccgagcg cgagcgcgtc ggccatcacc
     1801 aggcggccct ccgcgtcggt gttgagcacc tcgacggtct tgccgttctt catggtgagc
     1861 acgtcgcccg gacgggtggc gctgccggac ggcatgttct cggccgaggc gaggtagccg
     1921 gtgaccgcca ccttgacgcc gagatcggcc acggccgaca gcaccccgag caccgccgcc
     1981 gcgccggaca tgtcggtctt catggtgatc atgccgtcgg gcggcttgag cgagaggccg
     2041 cccgagtcga aggtgatgcc cttgccgacc agcaccacgt ggccgcgcgc accctcgggc
     2101 tggtaggaca gctcgatcag ccgcggcggg ttggcggcgc cctggccgac cccgacgatc
     2161 ccgccgaagc cctcccggcg caactgctcc tcgtcgcgga cggccacccg cacgtccttg
     2221 cccgccaccg ccgcgcgggc ggcctcggcc aggtcggcgg ggtgcagggc gttggcgggc
     2281 tcgttggaga ggtcgcgcgc caggttggtg gcggctgccc ggaccgtgcc ggcggccagc
     2341 gccccttcca gatcctggcg gtccgggtcg tcgccgctga cggccaggtc gagctgccgc
     2401 agcgcgtcgg tgctggcagc gccgccgtcg cgccgcggct gcttgtactt gtcgaaccgg
     2461 taggccgcca gcagcgcgcc ctcggcgacc gcccggaccg ctccgaccgg gtcgccgggg
     2521 agggcctgca ccagcgtggt gaccgcctgc ttggccccgg cggcatggcg cacgatcacc
     2581 ccggcggcac ggcgcagcgc ctccgcgtcc gcctcgccgc ggtcaccgag gccgactacc
     2641 agcagcgtgg cggcggccaa ccggccgagc gccggcaccg ccaccgcctc gccgagcttg
     2701 ccgctgaacc cgcgcgcctc cagcagcgac cgcagcggca caccgagcgc cgccgccgcc
     2761 tccgccccgg gacctgggtc caggccgccg aacaccggca ccaccagcag gtcggtcggc
     2821 gcgccagccg cgtcggcatc aaccagcgag acgttgagca tcggtcctcc caccactcca
     2881 tcgcacgaca agcctccagc tgaggcacca tagtctagtc ggctggcctg cgctgatggc
     2941 gcgggccgca atgagatttc ggaggaacgg ggtgcagatg gagcggcgca ggctggcgga
     3001 cctgatcgcc ttcgacgacg cccgcatggc caagcatcgc ctgttcgaga ccgaccgcac
     3061 gctcaccgac ctctactgcc tcgacgtcgg ccaggagcag ctcctgcgcg tccacgacga
     3121 cgccgacaag gtgatcctgg tgatggccgg ccgcgtcctc gcccgcgtcg acgacgacag
     3181 cgccgagttg acccagtacg agatcctgct gatcccagcc ggcgccaagc acgccctcaa
     3241 gaacctcggc cctgagcccg ccgtcctggt cgtcttcgtc ggcccccgcc tcgcctgacg
     3301 atcaccacga aaccgttacg gtctcgcaat aaggcggcta ttgcgtaatt tatctttctg
     3361 tggtattcta ggtagctatg ccggaattcc gttgctagca tcgccctcct tcccgggtcg
     3421 gcgatgtgat gaaggacgga gggattccga ctgatgcatg aacggcagcc cagccagacc
     3481 gcgatgatgg cggccgcggc acgagccgca caccttgtgg tggacaacga gccctacatc
     3541 ttccaggact cggtcgccat gcccctgctc ggagatttgg gcgaggagat cgtcgcctac
     3601 catcgcgcga gcggggatca cgtcgtgctg tccagcaccc gggcgcaggt caccgcccgc
     3661 agccgctaca ccgaagaccg cctggccgaa ctggtcctga ccgaccacgt gctgacccag
     3721 tatgtgatcc tcggcgccgg cctggacacc ttcgcctacc gcgccccgct ggcgctggcc
     3781 acccggctgc gggtcttcga agtcgaccat cccgccaccc agcggtggaa gcgcgacctg
     3841 ctcgccgccg cccagctcac ccccgtcgca cccgacggcc tggccttcgt cggcaccgac
     3901 ttcgaaaccg acccgctgac aggccgcctg accgccgcgg gcttcgaccc cgcccagccc
     3961 gccctggtga gctggctcgg cgtcaccttc tacctcaccc gcgacgccat cgtcgccacc
     4021 ctcgggacgc tcggcagctt ggcgcctggg acggaactgg tcgtcgagta cgccctccct
     4081 cccgaactcc gcgacgcgag gggcgcggcc tacgccgagt tcgccctccc cgccgccgcc
     4141 gagcgcggcg agccctggct gaccttcctc acccccgagg acatgtccac cctgctgcga
     4201 acccacggct tcgaggtcgt caagcacctc cgccagcacg acgcgatcga ccccgccctg
     4261 tggcagcgat ccgacgccct gcgccccgcc gacgtctgcc gcctcgtccg cgcggtggtc
     4321 gcgccgcgct gatccgcgtg gctggccgcg gtcagcagcg gctggggagg taggcggtgg
     4381 tcatcacggt ggtggcgctg atcgccgtgg ggagcaggcc ggtggtgtgc atccagtcgc
     4441 cgtagcgttg ccaggcggac tggtcgaggc agccgatcgg gcggtcgggc ggtgtcagca
     4501 gggtgagggt ggtggggacg ctggcctgga ggaatttggg ggtgtaggtg gtggctctgg
     4561 tcatcgccgt gacggcggcg ggctggtcgg cgcgggcggc gctggcgccg gccagcatgg
     4621 cggtgatgaa gcggcggacg gtggtggcgt aggcggggtc gccgcgcagg cgggtggtgt
     4681 tggcgaccag gaccagctcg tcgtagctgg ggacgccgag gcggtcgacg gggagggtga
     4741 cgggcgcgcg gccggtctgc tgggcgatct ggatggcttc gacgttgcgg tagccgccga
     4801 ggatcgcgtc gaccttgtgg gagagcaggg ccgggaccag gttgtagccg acgctgacgg
     4861 gggtgacgtc gccgtggctg aggccggcgc tgcgctccag ggtctggagg atggcgtcgt
     4921 ctgacgggat gccggtgatg ccgaccttgc ggcccttgag gcttgccggg gtggtgatgc
     4981 cgctggaggg cagggcgatc agggagttga gcggcaccgg gatcaccgag gcgaccgcgg
     5041 tgacagggag gtgcttggcg gcggcgaaga acaggtcggg ctcgtaggag atggcgaggt
     5101 cggccttgcg cagcgccacc agcttgaggg gggcgctcgg gtcggagggc gcgcgcatgc
     5161 gcaccgtcag gccggcctgc tggaacaggc ccttggactg ggcgtagtag aggccgacgt
     5221 ggtcggggtt gggcgtccag tcgagcgaca gggcgacctg ggtctggccc ggcctggcgc
     5281 cggcctgggc ctgccttgcc ggggagtttc cgcaggcggc gagcgccacg gccagggtgg
     5341 cggccaggac gaggggaaca acggctcgct tcatgggtgt agggcacctc cacttccatt
     5401 tccatttcca ccgccgcgcg cccaggggac ggccaggcgt tcggccaggg agaccagcag
     5461 gaacagggcg agcgagagca cggtcaggat gacgatcgct gcgaagatcc gggcggtgtc
     5521 gagattgggg gtggcctgca gcatcacgta gccgaggcca gcgttggagc cggcccattc
     5581 gccgaagacc gcgccaacgg cggcgtaggt ggcggcgacc cgcaggccgg agaagaccat
     5641 cgggagggcg gacggccact cgacccgctt gaggatcgcc catctgctgg cgtcaagggt
     5701 gcgcatcatc cggaccagct ccgggtcgac cgagcggagc ccatcggtgc cgttgacgac
     5761 gatcgggaag aagcagatca gcgcgacgat caccagcttg ggggcgaggt cgaagccgag
     5821 caggatcacc aggatcgggg ccagcaccac tactgggacg gtctgcgagg cgatcagcag
     5881 cgggtacagg gcgcggcgca gcgtcgccga caggtgcagc gccagcgcca gtgccagccc
     5941 ggtagcgcag gcgagcagga agccgagcac gacctcccgc agggtcaccc aggtcgccga
     6001 ggcaagcagc ccggcgtcgg tgtggaagtc cgcggcgacc gctgagggcg cgggcagcag
     6061 gtaggccggc acccgcagcg cccgcaccag cagctcccac agcaccaggc cgccgagcag
     6121 cagcgccag
//
LOCUS       DASWDC010000176         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_542937, whole genome shotgun sequence.
ACCESSION   DASWDC010000176 DASWDC010000000
VERSION     DASWDC010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542937"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VF995_05620"
     CDS             complement(<1..371)
                     /locus_tag="VF995_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX9343076.1"
                     /translation="MAALVLAAGASTRMGRPKQLALLKGRPLLEHVLGALSTSTDDGP
                     TDDGPVDAVVVALGAAADEVEGRLDLHGATPLRVEGWRGGMGEVLARALEDLGDRFGA
                     VVVCLGDQPLVSGAVVARLVAA"
     gene            complement(415..1200)
                     /locus_tag="VF995_05625"
     CDS             complement(415..1200)
                     /locus_tag="VF995_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX9343077.1"
                     /translation="MSASPSFEEMRDLIRADRRGALVTIVAGPGIGAKLLVSQEGTSG
                     SLGDAALDEAAAAIARELLPGEESAVREVDGRELFFDVYAPQPKLLIVGAVDFAAALA
                     HLAHLSGFRVAVVDARERFATLDRIPHADEVVVAWPHEYLAANPPDDATYAAVLTHEP
                     RFDDPTLTALLGSKVAYIGAMGSRRAHGERLERLRAAGFGEQELARISGPIGLDIGAK
                     TPEETAVSILAEVIAARHGRPGGRLSQREKSETVHTLKERGAI"
     gene            complement(1197..1511)
                     /locus_tag="VF995_05630"
     CDS             complement(1197..1511)
                     /locus_tag="VF995_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX9343078.1"
                     /translation="MRDVLDAIETWRGEGHRVAIATVTKVKGSAPRPPGAKMAISDDG
                     RWIGSVSGGCVEAAVLEEARRVLEGEPPRLVTFGITDEMVWDVGLACGGIIDVFIEPV
                     AE"
     gene            1716..2246
                     /locus_tag="VF995_05635"
     CDS             1716..2246
                     /locus_tag="VF995_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9343079.1"
                     /translation="MEVALLRRIACTVNGVRREAEVEPRQLLVYFLRETLGLTGTNVG
                     CDTSSCGACTVLVDGKSVKSCTMLAVQADGTEIQTIEGLATDGQLHPLQEAFRQHHAL
                     QCGYCTPGMVMAGLGLINEGADMSDEAVRLGLEGNLCRCTGYQNIVTAIQDAAGKLRG
                     EQVPAGAGTAGTQGGS"
     gene            2246..>3179
                     /locus_tag="VF995_05640"
     CDS             2246..>3179
                     /locus_tag="VF995_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9343080.1"
                     /translation="MTQVAEPEVTRYVGGGLLRKEDPELLTGQGRYVDDLSLPGMVWL
                     GFARSPLAHASLTRVDVSAARDLPGVVAAYSGEELAEAFAGPMPCAWPVADRTMPEEP
                     SGDPRVPDHWPVARERVRYAGEIVAVVVADSREHAIDGVEAVDVDYDELPVVIDMEAA
                     LADGAPVIYEQFGGNRTYTWSLANGEVDRVFAEAPVTVKERYYHPRLIPNAIEPRGVL
                     VQPSPATGEYTIWSATQIPHILRTLSALTLGIHEGKIRVVAPEVGGGFGSKLNAYAEE
                     FIALAVARKLGTPVKWVEERSEGYLATIHGRDVLQ"
BASE COUNT          459 a         1162 c         1127 g          431 t
ORIGIN      
        1 gccgccacca gccgggccac caccgccccg ctgaccagcg gctggtcgcc gaggcagacg
       61 accaccgcgc cgaagcggtc cccgaggtcc tcaagcgccc gcgcgaggac ctcgcccatc
      121 cccccacgcc agccctccac ccgcaacggg gtggccccgt gcaggtcgag gcgcccctcg
      181 acctcgtcgg cggcggcgcc gagcgcgacg acgaccgcat caaccggacc gtcatcggtt
      241 ggaccgtcat cggtggaggt ggagagggcg cccagcacgt gctccagcag cggacggccc
      301 ttcaggagcg cgagctgctt ggggcggccc atcctggtgg aggcgcccgc cgccaggacc
      361 agcgcggcca ccgggccgcg agggggagcc gccgcggacg ggtggccggc cacctcagat
      421 cgcgccccgt tccttcaggg tgtggacggt ctcggacttc tcacgctgcg acagccgccc
      481 gccggggcgt ccgtggcggg cggcgatcac ctcggcgagg atcgagacgg cggtctcctc
      541 gggcgtcttg gcgccgatgt ccaggccgat ggggccggag atgcgcgcca gctcctgctc
      601 gccgaagccg gcggcccgca ggcgctccag ccgctcgccg tgggcacggc ggctgcccat
      661 cgcgccgata taggcgacct tgctgcccag cagcgcggtc agggtggggt cgtcgaagcg
      721 cggctcgtgg gtcagcacgg ccgcgtaggt ggcgtcgtca ggcgggttgg cggccaggta
      781 ctcgtgcggc caggcgacca ccacctcgtc ggcgtggggg atgcggtcca aggtggcgaa
      841 gcgctcacgg gcgtccacca ccgcgacccg aaagccggag aggtgggcca ggtgggcgag
      901 ggcggcggcg aagtcgaccg ccccgacgat cagcagcttc ggctgcggcg cgtagacgtc
      961 gaagaacagc tcccggccgt ccacctcgcg caccgccgac tcctcgccgg gcaagagctc
     1021 ccgggcgatc gcggcggcgg cctcgtcgag cgccgcgtcg cccagggagc cgctggtgcc
     1081 ctcctggctg accagcagct tcgccccgat gccggggccg gcgacgatgg tgaccagcgc
     1141 gccgcgccgg tcagcgcgga tcaggtcgcg catctcctcg aacgacggcg aggcgctcat
     1201 tcggccaccg gctcgatgaa gacgtcgatg atcccgccgc aggccaggcc gacgtcccag
     1261 accatctcgt cggtgatgcc gaaggtgacc agtctgggcg gctcaccctc cagcacccgc
     1321 cgggcctcct ccagcaccgc tgcctcgacg cagccgccgg agacgctgcc gatccagcgg
     1381 ccgtcgtcgc tgatggccat cttggcgccc ggtgggcggg gcgccgaacc cttcaccttg
     1441 gtcaccgtcg cgatggcgac ccggtgcccc tctccccgcc aggtctcgat cgcatcgagc
     1501 acgtcccgca tgcggaaatc ccttcctcgg aaagcacctc tccggaccat ggctaccacc
     1561 acgatcggca cttggacctt ctgtttttgg tccgcgtgcc tgatgctatt gtgcgtctgc
     1621 gaccagtgtc ggccaagcag tcggtcgtcg tccgggttct gggcctaccc gaaatgccgt
     1681 gcgtgcccca gattccgtat atcgtgcgac cggagatgga ggtggccctt ttgaggcgca
     1741 tcgcttgcac ggtaaacggg gtccgacgcg aggcggaggt cgagcctcgc cagcttctgg
     1801 tctacttcct tcgcgagacg ctgggtctga ccgggaccaa cgtcggctgc gacacgtcgt
     1861 cgtgcggtgc ctgcaccgtg ctggtcgacg ggaagtcggt caagtcctgc accatgctgg
     1921 cggtgcaggc cgacgggacc gagatccaaa cgatcgaggg cctcgccacc gacgggcagc
     1981 tgcacccgct gcaggaggcg ttccgccagc accacgcgct gcagtgcggc tactgcaccc
     2041 ccggcatggt catggccggc ctcggcctga tcaacgaggg cgccgacatg agcgacgagg
     2101 cggtccgcct cggcctcgaa ggcaacctct gccgctgcac cggctaccag aacatcgtca
     2161 cggcaattca ggacgccgcc ggcaagctgc gcggcgagca ggtgccggca ggcgctggga
     2221 ccgcggggac gcaaggaggc tcctgatgac ccaggtggct gagcccgagg taacccgtta
     2281 cgtcggcggg ggactgctgc gcaaggaaga ccctgagctg ctgaccggcc agggccgcta
     2341 cgtcgacgac ctgagcctgc cggggatggt ctggctggga tttgcccgca gcccgctggc
     2401 gcacgccagc ctcacccggg tcgacgtctc cgccgcccgc gacctgcccg gcgtggtcgc
     2461 ggcctacagc ggcgaggagc tggccgaagc gttcgccggg ccgatgccgt gtgcctggcc
     2521 ggtcgccgac cggaccatgc cggaggagcc gagcggcgac ccgcgcgtgc ccgaccactg
     2581 gccggtcgcc cgcgagcggg tccgctacgc cggcgagatc gtcgcggtgg tggtcgccga
     2641 cagccgcgag cacgccatcg acggcgtcga ggcggtcgac gtcgactacg acgagctgcc
     2701 ggtggtcatc gacatggagg ccgccctcgc cgacggcgcc ccggtcatct acgagcagtt
     2761 cggcggcaac cggacctaca cctggtcgct cgccaacggc gaggtggacc gggtgttcgc
     2821 cgaagccccg gtgacggtga aggagcgcta ctaccacccg cgcctgatcc ccaacgcgat
     2881 cgagccgcgc ggcgtgctgg tgcagccgtc gcccgcgacc ggcgagtaca ccatctggtc
     2941 ggcgacgcag atcccgcaca tcctgcggac cctgtcggcg ctgacgctcg gcatccacga
     3001 gggcaagatc cgcgtggtcg cgcccgaggt cggcggcggc ttcggctcca agctgaacgc
     3061 ctacgccgag gagttcatcg ccctggcggt cgcccgcaag ctgggcacgc cggtcaagtg
     3121 ggtcgaggag cgctccgagg gctacctcgc caccatccac ggccgcgacg tgctccagg
//
LOCUS       DASWDC010000177         9677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_709827, whole genome shotgun sequence.
ACCESSION   DASWDC010000177 DASWDC010000000
VERSION     DASWDC010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9677
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709827"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1464)
                     /locus_tag="VF995_05645"
     CDS             complement(247..1464)
                     /locus_tag="VF995_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343081.1"
                     /translation="MRSGPTAETTGTTTRTPGGRASGASGASGASGGAVPRMSLAGDL
                     LTLVLTLWLVGGLFLDGWAHNTRPQLETFFTPWHAVFYSGFVATAAWVCWSVWVRRGV
                     PQGYGLALAGLAIFLLSGVGDMAWHLAFGIERNIAALLSPTHLGLFCGAFLLITTPLR
                     SQWADPDPALGAGRQVRLARLLPAVGSVALAGCLSAFIFMYLHPLYENIVSIGHRDGL
                     QAEFDAFQFVDVSQQNIEVGVPGFIFATAFLFGPLLFLARRWRPPAGSALVVIGVQAL
                     MMQALVGFRDPGLAVLGLLGAVAVEALLRLTGPSPSSPPRVRAFCALAPPLFWGVYFA
                     GIGLHDGGLGWKGEVWGGTLVWSGLSLLALTMVMWPPAVPVPVPGPGFGSGFGPGFGP
                     GPGFGPNRVGTGR"
     gene            1603..2469
                     /locus_tag="VF995_05650"
     CDS             1603..2469
                     /locus_tag="VF995_05650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9343082.1"
                     /translation="MGETRVSERERHEAPELVSVVVPTRDTARTIERCLASIRAQTHP
                     AIELIVVDNWSQDGTWETARRLADAAVQAGTERAAQRNLGIERAGGEWVLWIDADMEL
                     PPEVVERALAAAHTAGAVGVFIPEATTGSGFWTACRALERRCYHGEPRIEAPRLLRTS
                     ELRDLGGFDEHLAGTEDAELRTRLLARGAPLAWADTLILHHEGRLTLTGVARKRFYYG
                     KGIPAYRHAHPGAVSGQLGATLAAFWRHRRLLAADPPHAAGVIVLRTWEAAAYAAGAA
                     AGASARGKLARP"
     gene            complement(2440..3069)
                     /locus_tag="VF995_05655"
     CDS             complement(2440..3069)
                     /locus_tag="VF995_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEX9343083.1"
                     /translation="MEQSSQPPEQPSQQQPPAPGVPAVRASNQERDAAVQRLQAAFAE
                     GRLDDEEFDQRTATALRARTRAELDQLLADLPAAVRSGTLAPASQAPRLMLGFKGSIA
                     RRGRWRVPERSMTVAYKGSCELDLRAAELTEPVTTINALAYKGRVEVIVPRGVRVEAH
                     GSSYGGTVVDHRDEEPIADVPLVRVRGFAYKGSVEVRASASRSRELPAG"
     gene            3164..3898
                     /locus_tag="VF995_05660"
     CDS             3164..3898
                     /locus_tag="VF995_05660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9343084.1"
                     /translation="MRDAIRTGVSRLHRLIRPIPSERRFDYDRRLLVNPYLAEQEAGL
                     GDLYAAIERTGLSLGYPAWNLLYYTLLCSLPGTDPVVVETGTNHGFSTIVLAQALIDA
                     GAGGVVRTVDIDPGVVAVARGHAERAGVAERIRFHVGDSYEFLDRLPDEVSHVDFAFL
                     DGDHRAEHVIGEFERLYPLVAACDGKVYFDNTGRGGVAKALRHIHKVHGGNLVEFPSC
                     SWAPPGNAIWQPKRPKRPAPAARRRR"
     gene            3926..5047
                     /locus_tag="VF995_05665"
     CDS             3926..5047
                     /locus_tag="VF995_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343085.1"
                     /translation="MDAPFGPGVRSWKAATALLVVVLLLTAAAVIWLPRIRVPAATAD
                     RHSWQLFGRPAGAGNWVAGDGSYPVALADGRVAWLFGDSLVRRADGSDAMVHNSIVVQ
                     DRAGRLRTVAGGGAATPADLIAPVQPGTWLWPTGGFVEGGRLLVFAEEFAQPPSPTAG
                     SGTGAGFQFQATGRRYLVAFQLPGLTGGAPREVYPGPVAWGHAVLANKADGYVYVYGN
                     LERDGWTNLTYLARFPLGQSAGFWQFWDGSGFGPDPLTAAPLLGSGGTALVAKLGSVI
                     PAPDPGHGAPAAPAAPATAPEAPAGGGFAAFTIDPFGTTIDLRVAPAPQGPWSASQAL
                     YTVPEQHPYLPRAHVDRTGTIQLAYSVPDSRPRYLTARW"
     gene            5041..6024
                     /locus_tag="VF995_05670"
     CDS             5041..6024
                     /locus_tag="VF995_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9343086.1"
                     /translation="MVTRAGQRLTAAATGAGSGGVLVLGAAVLWGSTGTARALGPPGA
                     SPLSVGAVRILLGAAALLAVAWVRGGLRLQPRGWPLGAVAAGAGAVAAYQLAFFSAVA
                     RTGVAVGTIIAIGSAPPAAGLLAWLVRGERPGRRWMAATALAVAGGGLLAAAGQAVRF
                     RPAGAALALAAGAAYAVYALASKQLLSLERHAPVAVMAVLFAGGAVLLLPVLAREPLG
                     WVARPRGLLVALQLGLVATALAYALFAEGLARLSVATAATLSLAEPLTAALLGVVVLG
                     ERLTLAGVVGGLLVLTGLAALATAGPQPQSRAGTGTGTGTGGSWSAHGHGG"
     gene            6008..7180
                     /locus_tag="VF995_05675"
     CDS             6008..7180
                     /locus_tag="VF995_05675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9343087.1"
                     /translation="MAMEADVGVVGGGVIGMSVARELARAGVDRVVVLEREAAVGQGS
                     SSRANGGVRAQFTTKANIEFSRYSIGELEELERSSGLLEFRQTGYLLMTGTEAGERAL
                     REAWGLQRSLGVETRWLTPPEVLELAPFVRPEGLRAGTFHHRDGFLDPHGVVSALRLE
                     AGRLGVRVMTGAEVAAIRRLSKPGAGFELATTANGTGQTLRARWVVNAAGPAAAQVAA
                     LAGVELPVAPVRRNLAFAREREPGELIPMCVDLDTGVLVRRELRGGFVLAWSDPDDPP
                     SWETTVDPRFLEGLAQRVGHRFPFAEDLPIDQRQCWAGLYPETPDHHAIIGEEAEVPG
                     FVQCAGFGGHGVMHSPAAGRAVAELLTLGRCASFDLHPLRPSRFAEGDLVVETAVL"
     gene            complement(7206..8897)
                     /gene="aspT"
                     /locus_tag="VF995_05680"
     CDS             complement(7206..8897)
                     /gene="aspT"
                     /locus_tag="VF995_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0070906 - aspartate:alanine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter"
                     /protein_id="HEX9343088.1"
                     /translation="MHWLKHLLSETPLLALFASLAIGYLIGKIPFGPIKLGGVCGTLI
                     AAILIGQLGVSISDDLKTFAFALFIYALGYTGGPAFFSNLNRKGLRFGVLSIIEVVSV
                     LALVSGITWVLQLDRGTSAGLLAGAATESAVVGTASDALAKLGLPASQVQTLQANVAT
                     TYTISYLFGLVMIVLFTSQLGPMILRVNLREEAKRLWDRLDGGGKLEAGQSQALPALV
                     GRVYSVTAGAGRTIGEIERGLASDATVEVVRRGAQTLKVGPELRLALDDAVLVVGQRS
                     AVVDADDLIGPERDADGIGLVLQTRDVVVTNKTLNNHSLGELRATLAPEARHGVYVSG
                     VSRMDNPLPSLAETPLQQGDVVTVTGAPADVARVTPRIGYPSASGNATDFVYLGLGLT
                     LGLLIGMVSVKIAGVPLTLGTGGGALVAGLIFGWARAKHPTFGAFPGAAAQVVKDLGL
                     ATFIAVTGLSTGSQAWTLIGKYGISLPISGILITLVPASISLFVGRRLLKLDPPVLFG
                     GIAGQQCSTPAISSVLSAAGNSTPLIGYTVTYALSNVLLPLLGPVMVGIAGRIVS"
     gene            complement(8940..>9677)
                     /locus_tag="VF995_05685"
     CDS             complement(8940..>9677)
                     /locus_tag="VF995_05685"
                     /EC_number="2.6.1.1"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate transaminase/aspartate
                     4-decarboxylase"
                     /protein_id="HEX9343089.1"
                     /translation="DVYGTFVSGYRSLLSVVPRNTIGVYSFSKYFGCTGWRLGVVAVS
                     EDNIFDEKLARLPESDRTALATRYESITLDPAKLRFVDRMVADSRSVALNHTAGLSLP
                     QQVQMTLFALSALLDEQDAYKQATRSIIARRLHALTKGMGVPLPEDPLRAGYYAELDL
                     LAWAERHWGKDFADYMRANYEPTDMVFRLAEQGSVVLLNGGGFDGPDWSVRVSLANLP
                     MRAYEQIGQWLAEIGEEYQQAWQDQKR"
BASE COUNT         1305 a         3421 c         3623 g         1328 t
ORIGIN      
        1 acagcatggc ccaggacctc cccccggtcg gctgacgccg acgcactgcg ccatctggat
       61 acggctgtct ctggccgtcc cgatcagcgg catttcagaa aaacgccgcg tgtttggcag
      121 caaagggaca ttggcgctac aagggcaacg atgtcaagga tgatccacga tgcacggcgc
      181 ctgagggacc gcaacccctg cgcctgcagc tggacgatgc cgtcccgggc tcgtcagact
      241 gcgccgtcag cgtccagtgc cgacgcggtt gggcccgaaa ccggggccgg ggccgaaacc
      301 ggggccgaaa ccggagccga agccgggacc ggggaccggg accgggaccg ccggcggcca
      361 catcaccatg gtgagggcga gcaggctgag cccgctccag accagggtgc cgccccagac
      421 ctcgcccttc cagccgaggc cgccgtcgtg caggccgatc ccggcgaagt acacgcccca
      481 gaacagcggc ggcgcgaggg cgcagaaggc ccgcacccgc ggcggcgacg acggcgacgg
      541 accagtgagg cgtagcagcg cctcgaccgc gacggcgcca agtaggccga ggacggccag
      601 acctgggtcg cggaagccca cgagcgcctg catcatcagc gcctggacgc cgatcacgac
      661 cagggcggac ccggctggcg gccgccagcg gcgcgcgagg aacaggagcg gcccgaacag
      721 gaacgcggtg gcgaagatga acccgggcac gcccacctcg atgttctgct ggctgacgtc
      781 gacgaactgg aaggcgtcga attcggcctg aaggccatcc cggtgcccga tgctgacgat
      841 gttctcgtag agagggtgca ggtacatgaa gatgaaggcg gacaggcacc cggccagggc
      901 aaccgacccg accgccggca gcagccgcgc cagccgcacc tggcggccgg cgccgagcgc
      961 cgggtccggg tcggcccact gcgaccgcag cggcgtggtg atgagcagga acgccccgca
     1021 gaacaagccg aggtgcgtcg ggctgagcag cgcggcgatg ttccgctcga tcccgaacgc
     1081 caggtgccag gccatgtcgc cgaccccgga cagcaggaag atcgccaggc ccgccagcgc
     1141 cagcccatac ccctgcggca cgccccgccg cacccacacc gaccagcaca cccacgcggc
     1201 ggtcgcgacg aacccggagt agaacaccgc atgccagggg gtgaagaacg tctccagctg
     1261 cggccgggtg ttgtgcgccc acccgtccag gaacaacccc ccgaccagcc acagggtcag
     1321 caccagggtg agcaggtcgc cggccaagct catccgcggc acagcgccgc cgctggcgcc
     1381 gctggcgccg ctggcgccgc tggcgcggcc gccgggcgtc ctcgttgtcg ttcccgtcgt
     1441 ctcggcggtg ggtccggatc gcatctcgac ctccacacac tgcgtcgttt ccaggaccag
     1501 atctctgcgg ctaccctacg gcaggcgccg ccgggaccca tccccctccc cggtgatttt
     1561 ccgcctcgct ctccagaggg agtcggccta gagtcaaccc ccatgggtga aacgcgcgta
     1621 agcgaacgcg agcgacacga ggcacctgag ctggtgtcgg tggtggtccc cacccgcgac
     1681 acggccagga cgatcgagcg gtgcctcgcc tcgatccgcg cgcagaccca tcccgcgatc
     1741 gagctgatcg tggtcgacaa ctggagccag gacggcacct gggagaccgc ccgccggctc
     1801 gccgacgccg ccgtccaggc cggcacggaa cgagccgcgc agcgcaacct cggcatcgaa
     1861 cgggcaggtg gcgagtgggt cctgtggatc gacgccgaca tggagcttcc ccccgaggtg
     1921 gtcgagcgtg ccctggccgc cgcgcatacc gccggcgccg tcggggtgtt catccccgag
     1981 gccaccaccg gcagcggctt ctggaccgcc tgccgggccc ttgagcgccg ctgctaccac
     2041 ggcgagcccc ggatcgaggc gccccggctg ctgcgcacca gcgagctccg cgacctcggc
     2101 ggcttcgacg agcacctcgc cggcaccgag gatgccgagc tgcgcacccg cctgctcgcc
     2161 agaggcgcgc cgctcgcctg ggccgacacg ctgatcctgc accacgaagg ccgcctcacc
     2221 ctgaccggcg tcgcccgcaa gcgcttctac tacggcaagg gcatccccgc ctaccgccac
     2281 gcccaccccg gcgccgtctc cggccagctc ggcgccaccc tcgccgcgtt ctggcgccac
     2341 cgccgcctgc tcgccgccga tcccccgcac gccgccgggg tcatcgtgct gcgcacctgg
     2401 gaggccgccg cctacgccgc gggcgccgcc gccggggcgt cagcccgcgg gaagctcgcg
     2461 cgaccgtgag gctgaggcgc gaacctccac cgagcctttg taggcgaagc cgcgcacccg
     2521 gaccagcggg acgtcagcaa tcggctcctc gtcgcggtgg tcgacgacgg tgccgccgta
     2581 gctggagccg tgcgcctcga cccggacgcc gcgtgggacg atcacctcga cccggccctt
     2641 gtaggcgagc gcgttgatgg tggtgaccgg ttcggtcagc tcggcggcgc gcaggtccag
     2701 ctcgcagctg cccttgtagg cgacggtcat cgaccgctca gggacgcgcc agcggccgcg
     2761 ccgcgcgatc gaacccttga agccgagcat cagccggggc gcctggctcg ccggggcgag
     2821 ggtgccgctg cggacggcgg caggcaggtc ggcgagcagc tggtccagct cggcccgggt
     2881 ccgggcgcgc agggcggtgg cggtgcgctg gtcgaactcc tcgtcgtcga ggcggccctc
     2941 ggcgaaggcg gcctgcagcc gctgtacggc ggcgtcgcgt tcctggttgg aggcccgcac
     3001 cgcggggaca cccggcgccg gcggctgctg ctgtgatggc tgctctggcg gctggctgga
     3061 ttgctccatg ggctccagct tataagatgc gtctccccca tcttgggttt gtttgctgcg
     3121 gttgtgccgg gattcggcgt gtggagggag gcgtccggac agcatgaggg atgcgatccg
     3181 aactggcgtg tcgcggctgc accgcctgat ccgcccgatc ccgagcgagc gccgcttcga
     3241 ctatgaccgg cgcctgctgg tcaacccgta cctggccgag caggaagctg gcctcggcga
     3301 cctgtacgcg gcgatcgagc ggaccgggct gtcgctcggc tacccggcct ggaacctgct
     3361 gtactacacg ctgctgtgca gcctgcccgg gaccgacccg gtggtggtgg agaccggcac
     3421 caaccacggg ttctccacga tcgtactggc gcaggcgctg atcgacgcgg gtgccggcgg
     3481 ggtggtccgc accgtcgaca tcgacccggg ggtcgtcgcg gtggccaggg ggcacgcgga
     3541 gcgggccggg gtcgccgagc ggatccgctt ccacgtcggc gacagctacg agttcctcga
     3601 ccggctgccc gacgaggtca gccacgtgga cttcgccttc ctcgacggcg accaccgcgc
     3661 cgagcacgtg atcggcgagt tcgaacgcct ctacccgctg gtggctgcct gcgacggcaa
     3721 ggtctacttc gacaacaccg gccgcggcgg ggtcgccaag gcgctgcggc acatccacaa
     3781 ggtccacggc ggcaacctcg ttgagttccc gagctgctcg tgggcaccgc cgggcaacgc
     3841 catctggcag ccaaagcggc cgaagcggcc ggccccggcg gccagacgcc gccgctgagc
     3901 cctgagccgg gcgtacttta gtggcatgga cgcgccgttc ggtccagggg ttcggtcgtg
     3961 gaaggccgcg acggcgctgc tggtcgtggt gctgttgctg acggcggcgg cggtgatctg
     4021 gttgccacgg attcgggttc ctgccgccac cgcggaccgg cactcctggc agctgttcgg
     4081 ccgcccggcc ggggcgggga actgggtcgc cggggacggt tcctacccgg tcgcgctggc
     4141 ggacggcagg gtcgcgtggc tgttcggcga ttcgctggtc cgccgggccg acgggagcga
     4201 cgcgatggtc cacaactcga tcgtcgtcca ggatcgcgct ggccgccttc ggaccgtcgc
     4261 gggaggcggt gccgccacgc cggccgacct gatcgccccg gtgcagcccg gcacctggtt
     4321 gtggccgacc ggtgggtttg tggagggcgg gcggctgttg gtgttcgccg aggagttcgc
     4381 ccagccaccc agcccgacgg cggggagtgg caccggcgcc gggttccagt tccaggcgac
     4441 cggccgccgc tacctggtcg ccttccagct ccccggcctg accggcggcg cgccccggga
     4501 ggtctacccg gggccggtcg cctgggggca cgcggtgctg gcgaacaagg ccgacgggta
     4561 tgtgtatgtg tacggcaacc tggagcgcga cggctggacc aacctcacct acctggcgcg
     4621 gttcccgctc ggccagtccg ccgggttctg gcagttctgg gacggcagcg gcttcggccc
     4681 cgacccgctc accgccgcgc cgctgctggg gtctggcggc acggcgctgg tcgccaagct
     4741 cggcagcgtg atccccgcgc ccgatcccgg ccatggcgcc cctgccgccc ctgccgcccc
     4801 tgccacggct ccggaggccc cggcaggggg cggcttcgcc gcgttcacca tcgacccgtt
     4861 cgggaccacc atcgacctgc gggtcgcgcc ggccccgcag ggaccgtgga gtgccagcca
     4921 ggccctctac accgtccctg agcagcaccc gtacctgcct cgcgcccatg tcgaccggac
     4981 tgggacgatc cagctcgcgt attcggtgcc tgacagccgg ccccggtatc tgaccgcccg
     5041 atggtgaccc gcgcggggca gcggctcacc gccgccgcca ccggcgctgg ctccggcggc
     5101 gtgctggtgc tcggcgcggc ggtgctgtgg ggatcgaccg ggacggcgag ggcgctggga
     5161 ccgccggggg catcgccgct gtcggttggc gcggtgcgga tcctgctcgg ggctgccgcg
     5221 ctgctggcgg tggcctgggt gcgcggcggc ctccgcctcc agccgcgagg gtggccgctt
     5281 ggggcggtgg cggcgggggc gggggcggtc gccgcctacc agctcgcgtt cttctcagcg
     5341 gtggcgcgga ccggggtggc ggtcggcacg atcatcgcga tcggcagcgc gccgcccgcc
     5401 gcagggctgc tcgcctggct ggtccggggg gagcggccgg ggcggcgctg gatggcggcg
     5461 accgcgctgg ccgtcgccgg gggtgggctg ctcgccgccg ccggccaggc ggtccggttc
     5521 cggccggccg gggcggcgct ggcgctcgcc gccggggctg cctacgcggt gtatgccctg
     5581 gcgagcaagc agctcctcag cctggagcgc catgcgccgg tggcggtgat ggcggtgctg
     5641 ttcgccggcg gcgcggtgct gctgctgccg gtgctggcac gggagccgct tggctgggtc
     5701 gcccgtcccc gcggattgct ggtcgccctc cagctgggcc tggtcgccac cgcgctggcc
     5761 tacgccctgt tcgccgaggg gctggcgcgg ctgtcggtgg cgaccgcggc gacgctgtcg
     5821 ctggccgagc cgctgaccgc cgcgctgctc ggggtggtgg tgctcggcga gcgcctgacc
     5881 ctggctggcg tggtcggcgg gctgctggtc ctgaccggcc tggcggccct ggccacggcg
     5941 ggaccgcaac cccaatcgcg cgccggaacg ggaacgggga ccggaacggg agggagctgg
     6001 tcggcccatg gccatggagg ctgacgtcgg ggtggtcggc ggcggggtca tcgggatgtc
     6061 ggtggcgcgg gagcttgccc gtgcgggcgt cgaccgggtg gtggtgctgg agcgcgaggc
     6121 ggcggtcggg caggggtcgt cgtcgcgcgc gaacggcggc gtgcgggcgc agttcaccac
     6181 caaggcgaac atcgagttct cgcgctactc cattggagag ctggaggaac tggagcggag
     6241 cagcgggctg ctggagttcc ggcaaaccgg ctacctgctg atgaccggca ccgaggccgg
     6301 cgagcgtgcc ctgcgggagg catgggggct gcaacgctca ctgggcgtgg agacccgctg
     6361 gctcactccc ccggaggtct tggagttggc gccgtttgtg cgcccggagg ggctgcgggc
     6421 ggggaccttc caccaccgcg acggcttcct cgacccgcac ggggtcgttt cggcgctgcg
     6481 cctggaggcg ggcaggctgg gcgtgcgcgt catgaccggt gccgaggtgg ccgcgatccg
     6541 ccgcctgtcg aagccgggtg cgggcttcga gctggcgacg acggcgaacg ggaccggcca
     6601 gacgctgcgg gcccgctggg tcgtcaacgc cgccggaccc gccgccgcac aggtcgcggc
     6661 gctggccggg gtggagctgc cggtcgcgcc agtgcggcgc aacctcgcct ttgcccgcga
     6721 gcgggaacct ggggagctga tcccgatgtg cgtggacctc gacaccgggg tgctggtgcg
     6781 ccgcgagctg cgcggcggct tcgtgctcgc ctggtcggac cctgacgacc cgccgtcgtg
     6841 ggagacgacg gtggacccgc gcttcctgga ggggctggcg cagcgggtgg gtcaccggtt
     6901 ccccttcgcc gaggacctgc cgatcgatca gcggcagtgc tgggcgggcc tgtacccgga
     6961 gacccccgac caccacgcga tcatcggcga ggaggccgag gtccccgggt tcgtccagtg
     7021 cgccgggttc ggcggccacg gggtgatgca ctcccccgcc gccggcaggg cggtcgctga
     7081 gctgctcacc cttggccgct gcgccagctt cgacctgcac ccgctgcgcc cgtcccgctt
     7141 cgccgaaggc gacctggtgg tcgagacggc ggtgctgtag cggcgccccg ggcggcgcgg
     7201 gccgctcagc tgacgatgcg gccggcgata ccgaccatca ccgggccgag cagcggcagc
     7261 agcacgttcg acagcgcgta ggtgacggtg tagccgatca gcggggtgga gtttccggcc
     7321 gcgctcagca ccgagctgat cgccggggtg ctgcactgct gcccggcgat gccgccgaac
     7381 agcaccggcg ggtcgagctt gagcagccgc cgcccgacga acagcgagat gctcgcggga
     7441 accagggtga tcaggatgcc ggagatcggc agggagatgc cgtacttgcc gatcagcgtc
     7501 cacgcctgcg agccggtcga caggccggtc accgcgatga aggtggccag cccgagatcc
     7561 ttgacgacct gcgccgccgc tccggggaac gccccgaagg tggggtgctt ggcgcgggcc
     7621 cagccgaaga tcaggccggc gaccagcgcg ccgccgccgg tgccgagcgt cagcggcacg
     7681 cccgcgatct tgaccgacac catgccgatg agcaggccga gcgtcaggcc gaggccgagg
     7741 tagacgaaat cagtcgcgtt gccgctcgcg gacgggtagc cgatccgcgg cgtgacccgg
     7801 gccacgtcgg ccggggcgcc ggtgacggtc accacgtcgc cttgctgcag cggggtctcg
     7861 gccaggctcg gcagggggtt gtccatgcgg ctgacgccgc tgacgtagac gccgtggcgg
     7921 gcctccggcg ccagtgtcgc gcgcagctcg ccgagcgagt ggttgttcag cgtcttgttg
     7981 gtgaccacca cgtcgcgggt ctgcaggacc agcccgatcc cgtcggcgtc ccgctccggc
     8041 ccgatcaggt cgtcggcgtc gaccaccgcg ctgcgctggc cgaccaccag caccgcgtcg
     8101 tccagggcga gccgcagctc ggggcccacc ttgagggtct gcgcgccgcg gcggaccacc
     8161 tcgacggtgg cgtcggaggc gagcccccgc tcgatctcgc cgatggtgcg gccggcaccg
     8221 gcggtgacgc tgtagacccg gccgaccagg gcgggcagcg cctgcgactg gcccgcttcg
     8281 agcttgccgc cgccgtcgag acggtcccac agccgcttgg cctcctcgcg caggttgacg
     8341 cgcaggatca tgggcccgag ctggctggtg aacagcacga tcatgaccag gccgaagagg
     8401 tagctgatgg tgtaggtggt ggcgacgttg gcctgcaggg tctggacctg gctggcggga
     8461 agcccaagct tggcgagcgc gtcggaggcg gtgccgacca ctgcggactc ggtggcggca
     8521 cccgccagca gcccggcgga ggtgccgcgg tcgagctgca gcacccaggt gatccccgag
     8581 accagggcga ggacgctcac cacctcgatg atcgacagca cgccgaagcg gagccccttg
     8641 cggttcaggt tggagaagaa ggcggggccg ccggtgtagc cgagggcgta gatgaacagc
     8701 gcgaaggcga aggtcttcag gtcgtcgctg atgctgacgc cgagctggcc gatcaggatc
     8761 gccgcgatca gcgtgccgca gaccccgccg agcttgatcg gcccgaaggg gatcttgccg
     8821 atcaggtacc cgatcgcgag cgaggcgaac agcgccagca gcggtgtctc gctcagcagg
     8881 tgcttgagcc agtgcatctg ccctctccct ctatggcggt ggtcagctta tggcggtggt
     8941 cagcgtttct ggtcctgcca ggcctgctgg tattcctcgc cgatctcagc cagccactgg
     9001 ccgatctgct cgtaggcccg catcgggagg ttggcgagcg agacccgcac cgaccagtcc
     9061 gggccgtcga agccgccgcc gttgagcaac accaccgagc cctgctcggc gaggcggaag
     9121 accatgtcgg tcggctcgta gttcgcgcgc atgtagtcgg cgaagtcctt gccccagtgg
     9181 cgctccgccc aggccagcag gtccagctcc gcgtagtagc cggcgcgcag cgggtcctcc
     9241 ggcaatggca cgcccatgcc cttggtgagg gcgtgcagcc gccgcgcgat gatcgaacgg
     9301 gtggcctgct tgtaggcgtc ctgctcgtcg agcagggccg acagcgcgaa cagggtcatc
     9361 tgcacctgct gcggcagcga cagcccggcg gtgtggttga gggcgaccga gcgggagtcg
     9421 gccaccatcc ggtcgacgaa cctgagtttg gcggggtcca gggtgatcga ctcgtagcgc
     9481 gtggccagcg ccgtgcggtc ggactcgggg aggcgcgcca gcttctcgtc gaagatgttg
     9541 tcctcgctga cggcgaccac gccgaggcgc cagccggtgc agccgaagta cttggagaac
     9601 gagtagaccc cgatggtgtt gcgcgggacc accgacagca gcgagcggta gccgctgacg
     9661 aaggtgccgt agacgtc
//
LOCUS       DASWDC010000178        14003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_709853, whole genome shotgun sequence.
ACCESSION   DASWDC010000178 DASWDC010000000
VERSION     DASWDC010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14003
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709853"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VF995_05690"
     CDS             complement(<1..523)
                     /locus_tag="VF995_05690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9343090.1"
                     /translation="MDLRAAMSQFATGVTVVTAATPAGEPLGSTANSVTSVSLSPPLL
                     LVWLNNDSDTLRGVLDTKAFAINVLSSGQHHLARTLAGKGAHHKRQHWERVPWSAGST
                     GSPLLGGALVTIECRLDAVLDGGDHRIVLGEVAGIRLHGDGDDGEAAERAPLLFFRGA
                     FHSLPAPAPAAEPC"
     gene            820..1854
                     /locus_tag="VF995_05695"
     CDS             820..1854
                     /locus_tag="VF995_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343091.1"
                     /translation="MRRLRSRRLRFAAVSILAGACLLGALSPAFATEDPPANSTDWGT
                     VVPDTPGPQADAWIASVKGLAGLVNTTLFEAHDPATPDAPKTRGGLEDGISIPFLGYV
                     SALGTVALANKLPAGSDGAPDVPMPGAAYAKASGGEITIGLPYVPNPFPGGTQLSPVG
                     LYADGIQTEAKVMPGKPVAFSGKFGDFSLSSFGFNIISLPLEWPVNFGVRIPQDRWLP
                     AIAEVMTKEEVTTNSLGQPTKDSDGNYVFDPKATAGYVNAGHVSVLGTDVADATVGHA
                     AVMGGAPLAPPTTLPKPPVTTMAPTTTMAPVTTMTPVTTMAPTTTEAPTTTSDAGPAA
                     VRSQDAKSTR"
     gene            2028..2972
                     /locus_tag="VF995_05700"
     CDS             2028..2972
                     /locus_tag="VF995_05700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX9343092.1"
                     /translation="MLLPLLLAAGCSGGGGGGGGGGGGTREQAALPTVSGPFGQKPSI
                     VIPAGTPSTQPRVRTVIDGTGPEVEKGQVVVADVGIKVWQGNREYLNSYDTKQPTTIA
                     LGGQYVSPTWDAALLGKRAGSRVVLVSPATQGFGPGGMAPANVSPTDTLIVVFDIIGS
                     YDPKAEVRGPSASVTRGGDAALPAVAGKAGQVPAITVPKGVPAPSRLVDETLVRGQGP
                     VVQKGQTVVVQYTSAVWERGSIFDSTLRRRGPNGFVLDSTSTLPGWTQGLVGKRVGSR
                     VLLVVPRSMGAGFQHTLGGAVAPPNSTMIYSIDIIDAR"
     gene            3267..3407
                     /locus_tag="VF995_05705"
     CDS             3267..3407
                     /locus_tag="VF995_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343093.1"
                     /translation="MNRNRLANLFRGRGEFQTLVMILAGWVLVTAALAAVLGLLHRIA
                     PS"
     gene            complement(3506..3961)
                     /locus_tag="VF995_05710"
     CDS             complement(3506..3961)
                     /locus_tag="VF995_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9343094.1"
                     /translation="MRVSAKADYAVRAVAELAAGGHGPIKGERIADAQKIPLKFLENI
                     LSELKHGGVVQSQRGAEGGYWLARPAEQITLAEIIRVVDGPLANVRGARPEAVEYAGS
                     AKHLLDVWIAVRSSLRIVLERVTVADLIAGNLPLVVHELLEHPDAWVSH"
     gene            4027..4692
                     /locus_tag="VF995_05715"
     CDS             4027..4692
                     /locus_tag="VF995_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX9343095.1"
                     /translation="MARNVEAVLFDLDGVLVDSEPVWSIAEREITEWLGGTWGPQVKA
                     ALVGNALDEASRLLLEFTGADRPVSEVAARLGSRIAELFEADGGLTVQAGAAELLAGL
                     RAEGVPLALVSSSSRRLVEAALRLVGREHFQVTIAGDEVTERKPLPAPYLKAAAHLGV
                     DPARCVVIEDSPTGVRAGEAAGCVVVAVPDHQLVTIPPGPRRHVVASLAAVEPAWLLS
                     LAA"
     gene            4817..5821
                     /locus_tag="VF995_05720"
     CDS             4817..5821
                     /locus_tag="VF995_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9343096.1"
                     /translation="MIRVKLGAVLLLAVALVAAGCGSTSSGGGGGTAKLTLVAYSTPR
                     EAYQALIPAFQATTAGKGVTFSQSYGASGDQSRAVLAGLPADVAEFSLAPDMTKLVDA
                     NLVAADWNAGPAKGMVTDSVVVLMVRKGNPKGIHTWNDLVKPGVQVITPNPFSSGGAR
                     WNVMAAYGAQIKQGKTPAQATDYLLQLFKNVPVQDKSAREATQTFVGGKGDVEIAYEN
                     EAIAARQAGQPVDYVVPDQTILIENPIAVTTKSKHQTQAKAFVDFLESAAGQQIFAKK
                     GYRPVDPAAARAAGFPTPPGLFTIADLGGWKDVAKQFFDKDSGVMAGIEKQVGVSLGS
                     "
     gene            5919..6701
                     /gene="cysT"
                     /locus_tag="VF995_05725"
     CDS             5919..6701
                     /gene="cysT"
                     /locus_tag="VF995_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX9343097.1"
                     /translation="MGSGTALLRGVVTTYLSLIVLLPLAAVVWRSAEGGRAAFWHEAT
                     APQALAALKLTFLLSLGVTAINLVAGTLIAWVLVRDEFPGKRVVDALIDLPFALPTIV
                     AGAVLLALYGQHSPVGLNLAYTRAGVLLALAFETLPFVVRAVQPVLLTMEPDMEEAAR
                     CLGAGPLVTFRRIVLPTIAPAMLAGAALGFARAVGEFGSVVLISGNLPYKTEMAAVHV
                     FTLVESDDPAGAAAVSVVLLALSLVVLAAISLLQRKGTRHAD"
     gene            6691..7539
                     /gene="cysW"
                     /locus_tag="VF995_05730"
     CDS             6691..7539
                     /gene="cysW"
                     /locus_tag="VF995_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX9343098.1"
                     /translation="MLTDATATAPTAPRATGSRRGSRVALRTAALGYLALVLVAPVGA
                     IVWRTFAHGLAPVWRSVTTPDALHAFWLTVLVAGIAVPLNTVFGIACAVVLVRHRFPG
                     KALLNAVIDLPFAISPVVIGLALVLVYGQDGWFGNWLVTHGVQVIFAVPGMLVATVFV
                     SLPYVVREVTPVLREVGTEQEEAAWTLGARPLRAFLRVTLPAIRWGVAYGVVLTTARA
                     IGEFGAVSIVSGRLAGQTETLTLLVEDRFEQFDLAGAYAASLLLAALALLTLLAMTRL
                     RKKDGP"
     gene            7539..8567
                     /locus_tag="VF995_05735"
     CDS             7539..8567
                     /locus_tag="VF995_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEX9343099.1"
                     /translation="MSIEARNLTKRFGTFTALDDVSVLIPSGSLTALLGPSGSGKSTL
                     LRVVAGLEQPDSGAVLIDGADAASVPVQRREVGFVFQHYAAFKHMTVRENVAFGLKLR
                     RRPKAEVRARVDQLLELVQIEGFAGRYPAQLSGGQRQRMALARALAVEPRVLLLDEPF
                     GALDARVRKELRAWLRRLHDEVHVTTVLVTHDQEEAMEVADRIVVMNHGRIEQIGDPR
                     SLYEQPANDFVMSFVGPVTRLGGDLLRPHDVALLPAPAEGAREAMIERLVHLGFEVRA
                     EVMLADGQRLLVQLSREEAELLDLAHGQIVYVRPTRGRALASAAPAAGPTGWPSGRGS
                     PKAGVRPA"
     gene            8722..9477
                     /locus_tag="VF995_05740"
     CDS             8722..9477
                     /locus_tag="VF995_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX9343100.1"
                     /translation="MVLAVAIVVMVVLFAFGAWTAVGFATLRSAERHQSFAFTGSRLV
                     VGTSNGSIHVTAGQAGRVEVDTHLRYSQLNPPHPTVRADGGQLTLKDGCPNGVVIFCD
                     VSFDLRVPAALALQLTTSNGAVAVSGVTGALDVHTSNGSITADGATGQLSLRTSNGAI
                     RARDLHTAVVDATTSNGSVTVSFLTTPTHVAVNTSNGAVRVTVPGGSGPYRVDVTTDN
                     GSRTVDVPTDPNATRTISVRTSNGSVHVLPAAS"
     gene            9702..10925
                     /locus_tag="VF995_05745"
     CDS             9702..10925
                     /locus_tag="VF995_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9343101.1"
                     /translation="MSRTHPAAIRIAGFKIVVSWTLSIVVALIAWSLATQLLPADFPG
                     RGQNAYWAAAAVTTALFLASLLAHELAHAVLARQVAGVEVEDITFWLFGGMSRMRGEL
                     PTPWTQLLVTAAGPAASLLLGGAFWLAAVVLAAVGAGGLPAGAALWLALMNLALAVFN
                     LIPGAPLDGGRMLHALLWRFQGSRARGALTAARVGQGTGMVLVGLGLVELLVIPGNAT
                     GGLWSALIGWFIIGAARNEARVATARLRLGERRVREIMTPDPAVGPGWYTVDAFLDTF
                     ASRHRQAAFPVRDFDGRLVGVVPMTLLGEVPPERRGLLRVRDLAIPLSSLVVAHPDDL
                     AVELALRLAGARQRSALVVEGERVAGLVSPSNLADPPRRAAGLAAGGAGAGGAGAGAT
                     PAPLPTSAEPRSEPQ"
     gene            10922..11224
                     /locus_tag="VF995_05750"
     CDS             10922..11224
                     /locus_tag="VF995_05750"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEX9343102.1"
                     /translation="MSPALKGLARGARRAGILIAGLALVAGGTVLLVLPGPGIALILA
                     GLAVLATEFHWARRLLAWARERARQVADRARQRTRTRTRTRTRTRSGSGSGSGRPG"
     gene            complement(11221..11826)
                     /locus_tag="VF995_05755"
     CDS             complement(11221..11826)
                     /locus_tag="VF995_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343103.1"
                     /translation="MDRPPQRPPQRPPQRARVRLAAGGGAVALALALALVPLVGARVA
                     AGPLLGLGAAGVALAAVAAWRFTGVAPVAVAVLVAEYGLSLYHRQGGGPFDGRAPLFA
                     ADYLLLAELANWSREANPLVRDETPVLAARLALLGATTALAAALGALVLVTAELPLVG
                     AVARLALGLLAAVTAIAVAAALAAWHAARGPADRNGNGPGG"
     gene            complement(11861..13258)
                     /locus_tag="VF995_05760"
     CDS             complement(11861..13258)
                     /locus_tag="VF995_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9343104.1"
                     /translation="MRQPTAKLGGYVGLAALLLLAGLTAGQPELVAVAAPFALLVIIG
                     QVAARTPEIAVTAEADTDRAVEGDEVIVTLTLVAAAPVSRLEALLVVPDGLALANAAV
                     ELPARALRLGRGEIRTVALRLRCDHWGAYQLGTVLLRAHDPLRLLRWEWPVRTSLPLV
                     VFPQPETLRMLVHPFETQVFTGNQVARQRGDGIEFADVRPFQPGDRARSINWRATARR
                     GDPWVNQRHPERNTDVVLFLDTFADVPASGVGTGPAATGPPGLQRGTLDRAVGAAASL
                     ASAYLARRDRVGLVGFGGVVRWLQPGSGQAALYRLLDTLMETQIFATVGWRNIHQLPP
                     RTLPPKALILALTPLLDERATAALFDVLARGYDLAVIDVSPLVPRDGDRPAAPGRGHA
                     RRRLALGRRSGAGPVLDELARRLWALERAALRHRYQQLGVPVVDWPPGVPAEQVVYEL
                     ELSRRRSSARSVPSP"
     gene            complement(13284..>14003)
                     /locus_tag="VF995_05765"
     CDS             complement(13284..>14003)
                     /locus_tag="VF995_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9343105.1"
                     /translation="QAALLEGMQEQQVTIEGTTHPLERPFLVLATQNPVEYEGTYPLP
                     EAQLDRFLLRLAVGYPSPEAEWAMVTRRLERGSDELRLDPVATPADLLAAQRAIERVH
                     VGESVGRYLIELVGATRASPRVQVGASPRGTLALLKAARANAAVAGRDFVTPEDVKAV
                     AVPALAHRLILRPELWVRRVRTEDVVTELLDQVPTPPALPDPVDTPGSPRASSDRFET
                     GGPPSGRGVFGMPEPGDPALR"
BASE COUNT         1929 a         4992 c         5094 g         1988 t
ORIGIN      
        1 gacagggctc ggcggcgggc gccggggccg ggaggctgtg gaaggcgccg cggaagaaca
       61 gcagcggcgc ccgctcggct gcctcgccgt cgtcgccgtc gccgtggagc cggatgccgg
      121 ccacctcgcc gaggacgatg cggtggtcgc cgccgtcgag cacggcgtcc agccggcatt
      181 cgatggtcac cagcgccccg ccgagcaggg ggctgccggt cgagccggcc gaccagggga
      241 ccctctccca gtgctggcgt ttgtggtggg cgcccttgcc ggcgagcgtg cgtgccaggt
      301 ggtgctggcc gctggacagc acgttgatgg cgaacgcctt ggtgtcgagc accccgcgga
      361 gcgtgtcgct gtcgttgttc agccagacca gcagcagtgg tggcgacagc gacaccgagg
      421 tcaccgagtt cgcggtcgaa ccgagcggct cgccggcggg ggtcgcggcg gtcaccaccg
      481 tgacaccggt ggcgaactgg ctcatggcgg cgcgaaggtc catggcgcta gagcatgccg
      541 gggcaaggcg cgctttgtca actaaattca tagacttaat gggaatagtc cactcacccc
      601 agagggggaa atcgaggtgt ccatcactgt ccgtggcgga accctcactt acgatgtctg
      661 acgcgatccg gccagtcggt gcattgctgc ccgcatagaa gggctgacgt caaaccgtcg
      721 attctgcggc acgacctggc gcctcggatc gcatcgatgg ccgtcgacgc ctgcacatcc
      781 gtcagcccgt cgcggccgca accctcaagg aggacctgca tgagaaggtt gaggtcccgg
      841 cggctacgct tcgccgccgt cagcatcctc gccggggcgt gcctgctcgg cgcactgtcc
      901 ccggcgttcg cgacggagga ccccccagcg aactcgacgg actggggcac ggtcgttccc
      961 gacactcccg ggccgcaggc cgacgcgtgg atcgcgagcg tcaaggggct cgctggcctg
     1021 gtgaacacca ccctgttcga ggcgcacgat ccggcgaccc cggatgcgcc gaagaccagg
     1081 ggcggcctcg aggacggcat cagcatcccg ttcctcggct acgtcagcgc gcttggcacc
     1141 gtcgccctcg cgaacaagct gccggctggc agcgacggcg cgccggacgt tcccatgcca
     1201 ggcgcggcat acgccaaggc cagcggcggg gagatcacca tcggcctgcc gtacgtcccg
     1261 aacccgttcc caggcggcac gcagctgtcg ccggtgggcc tgtacgccga cgggatccag
     1321 accgaggcga aggtgatgcc cggcaagccg gtcgcgttca gcggcaagtt cggggacttc
     1381 agcctgtcgt cgttcggctt caacatcatc agcctcccgc tggaatggcc ggtcaacttc
     1441 ggggtccgca tcccgcagga ccggtggctg ccagcgatcg ccgaggtgat gaccaaggag
     1501 gaggtgacca ccaactccct ggggcagccg accaaggaca gcgacggcaa ctacgtcttc
     1561 gacccgaagg cgaccgccgg ctacgtcaac gctggccacg tcagcgtcct cggaacggat
     1621 gtcgctgacg cgaccgtcgg gcacgccgcg gtgatgggcg gcgcgccact ggcgccaccg
     1681 acgacgctgc cgaagcctcc ggtgaccacg atggcgccga ccacgaccat ggcgccagtg
     1741 accaccatga cgccagtgac caccatggcg ccgacgacga ccgaggcgcc gacgaccacc
     1801 agcgatgccg gaccggcagc cgtccggtca caggacgcga agtccacgag gtagataagc
     1861 tacccactgc tgcacgcggc ccgtccccgc gggtgaccct gaccggctgc ccacggggac
     1921 ggcgtcgcgc gttgtccgag ttatccgata tgagtcagtg gagtcccccg ccagatcacg
     1981 agccgatgaa tcgagggtgt tgcctggtgc gccgtttcgc tgtcctgatg ttgctgccgt
     2041 tgctgctcgc cgccggatgc agcggtggtg gcggtggtgg cggtggtggc ggtggtggca
     2101 cgcgcgagca ggccgcgctg cccaccgtgt cgggtccgtt cggccagaag ccgtccattg
     2161 tcatccccgc cggaacgccg agcacgcagc cgcgcgtgag gaccgttatc gatgggaccg
     2221 gcccggaggt cgagaagggg caggtcgtcg tcgccgatgt gggcatcaag gtgtggcagg
     2281 gcaaccggga gtacctcaac tcctatgaca ccaagcagcc gaccacgatc gcgctcggcg
     2341 gccagtacgt ctcccccacc tgggacgccg ccctgctggg caagcgggcc gggagccggg
     2401 tggtgctggt cagcccggcc acccagggat tcgggcccgg cggtatggcc ccggcgaacg
     2461 tgtcgcccac cgacacgctg atcgtcgtct tcgacatcat cggcagctac gacccgaagg
     2521 ccgaggtgcg tggtccctcc gcctccgtga cgcggggcgg cgacgcggcg ctgccggcgg
     2581 tcgcgggcaa ggccgggcag gtcccagcga tcactgtgcc gaagggcgtg ccggctccca
     2641 gcaggctggt cgacgagacc ctggtgcggg gtcagggccc ggtggtgcaa aaggggcaga
     2701 cggtggtggt gcagtacacc agcgcggtgt gggagcgcgg cagcatcttc gattcgaccc
     2761 tccggcgtcg cgggcccaac ggcttcgtgc tcgattccac cagcacgctg ccgggctgga
     2821 cgcaagggct ggtcggcaag cgggtcggga gccgggtcct gctggtggtc cccaggtcga
     2881 tgggggccgg gttccagcac acccttggcg gggcggtcgc accgcccaac tccacgatga
     2941 tctacagcat cgacatcatc gacgcccgct gagcctgccc actgacccaa gcggttctac
     3001 cgatttcgtc gtgccggcgg gcgcgtcgcc tccggcgccg ctaccatggc cctgcggtgc
     3061 ttgccttgcc ggcggcggcc tcaggtcgtg ccggcgggct cgccgactgg gccggtgcgc
     3121 cgcctggcgt cgccggcccg gagcgccgca accccgacca tgcgcgtcga acccacccgg
     3181 gtcgtgccgt cctgcgcggc ccgggtgtgg gcaccggctc gcgaggacga cacgagttcc
     3241 agaccgacca aaggaaacat cacactatga accgcaaccg gctggccaac ctcttccggg
     3301 gccgtggcga gttccagacg ctggtcatga tcctcgccgg ctgggtcctg gtcaccgccg
     3361 cgctggcggc cgtgctgggc ttgctgcacc ggatcgcgcc ctcgtaaccg gagtcgttcg
     3421 aaccgcccac gtcctcggtc ctcggccccg ctgggcagcg gctacgtcgt agcccggcgg
     3481 ggccgaggca tgtccgagtc ggcggtcagt gggagaccca ggcgtctggg tgctcgagca
     3541 gctcgtggac gaccagcggc aggttgccag cgatcaggtc ggcgacggtg acccgctcca
     3601 ggacgatgcg gaggctcgac cgcaccgcga tccagacgtc gagcagatgc ttggcgctgc
     3661 cggcgtactc gaccgcttcg ggccgcgccc cgcggacgtt ggcgagcggg ccgtcgacca
     3721 cccggatgat ctcggccagg gtgatctgct cggccgggcg ggcaagccag tagccgccct
     3781 cggcgccgcg ctggctctgg acgacgccgc cgtgcttgag ctcagagagg atgttctcca
     3841 ggaacttcag ggggattttc tgggcgtcgg cgatccgctc ccccttgatc ggcccgtgac
     3901 cgccggcggc cagttcggcc accgcccgca cggcatagtc cgccttcgca gagactcgca
     3961 tgccgctatt ctccatgaga atgccctggc aggggaatca gtagtcgaaa aaggagacat
     4021 atcaccatgg cacggaacgt cgaggcggtc ctgttcgacc tcgacggcgt gctggtggac
     4081 tccgagccgg tctggagcat cgccgagcgt gagatcaccg aatggctcgg cgggacctgg
     4141 ggcccgcagg tcaaggcggc cctggtcggc aacgcgctgg acgaggcgtc gcggctgctc
     4201 ctcgaattca ccggcgccga ccggccggtc agtgaggtcg ctgcgcggct cggcagccgc
     4261 atcgccgagc tgttcgaggc cgacggcggc ctgacggtgc aggccggcgc ggccgaactg
     4321 ctcgccggac tgcgcgccga gggggtgccg ctggcgctgg tgtcgtcctc gtcgcggcgc
     4381 ctggtcgagg ccgctcttcg cctggtcggc cgggaacact tccaggtcac catcgccggc
     4441 gacgaggtga ccgagcgcaa gcccctgccc gcgccctatc tgaaggccgc cgcccacctc
     4501 ggcgtcgacc cggctcgctg tgtggtgatc gaggactcac ccaccggcgt gcgcgccggc
     4561 gaggcggccg gttgcgtggt ggtggcggtc cccgaccatc agctggtgac gatcccgccc
     4621 ggcccccggc gccacgtggt ggcatcgctg gcggcggtcg agcccgcctg gctgctgagc
     4681 ctcgccgcct gatgacgtag gcgatattca tgcagatgac ggagcgaccg gcgccgtacg
     4741 ctctggcatt ctctagttaa ttgatggatg agatatacat ctcaccgatg ggcaagctgt
     4801 ggaagggagt gccttcatga ttcgggtgaa gctcggcgct gtgctgctgc tggcggtcgc
     4861 actggtggcg gccggatgcg gcagcacgag ctctgggggt ggcggcggta cggccaagct
     4921 caccctagtt gcgtactcga caccacgcga ggcgtaccag gcgctcatcc ccgcgttcca
     4981 ggcgaccacc gccgggaagg gcgtcacctt cagccagtcc tacggcgcct ccggcgacca
     5041 gagccgcgcg gtgctcgccg ggctgcccgc cgacgtggcc gagttctcgc tcgcgcccga
     5101 catgaccaag ctggtcgacg cgaacctggt cgccgccgac tggaacgccg gccccgccaa
     5161 gggcatggtc accgactcgg tggtcgtgct gatggtccgc aagggcaacc ccaagggcat
     5221 ccacacctgg aacgacctgg tcaagcccgg cgtccaggtg atcaccccga acccgttcag
     5281 ctccgggggg gcccgctgga acgtgatggc cgcctacggc gcccagatca agcagggtaa
     5341 gaccccggcg caggccaccg actacctgct gcagctgttc aagaacgtgc cggtgcagga
     5401 caagtcggcc cgtgaggcca cccagacctt cgtcggcggc aagggcgacg tggagatcgc
     5461 ctacgagaac gaggcgatcg ccgccaggca ggccggccag ccggtcgact atgtggtccc
     5521 cgaccagacg atcctgatcg agaacccgat cgcggtcacc accaagagca agcaccagac
     5581 ccaggccaag gccttcgtcg acttcctgga aagcgccgcc ggccagcaga tcttcgccaa
     5641 gaagggctac cggccggtcg accccgccgc cgccagggcc gccgggttcc ccaccccccc
     5701 ggggctgttc accatcgccg acctgggcgg ctggaaggac gtggccaagc agttcttcga
     5761 caaggacagc ggcgtgatgg ccgggatcga gaagcaggtg ggagtgtcgc ttggcagttg
     5821 acgccggcac cgccgtcccg cggccggaag gagcgcgggc cgattccatg gtggccgcgg
     5881 ggccccccga tggatcaggt cttcgggaca gcctccgggt gggcagtggg acggccctgc
     5941 tgcgcggggt cgtcaccacc tacctcagcc tgatcgtgct gctgccgctg gccgcggtgg
     6001 tgtggcgctc cgccgagggc ggcagggcgg cgttctggca cgaggcgacc gccccgcagg
     6061 cgctggcggc gctcaagctg accttcctgt tgtcgctcgg tgtgaccgcg atcaacctgg
     6121 tggccggcac gctgatcgcc tgggtgctgg tgcgcgacga gttccccggc aagcgcgtgg
     6181 tcgacgcgct catcgacctg ccgttcgcgc tccccacgat cgtcgccggc gcggtgctgc
     6241 tggcgctcta cggccagcac agcccggtcg gcctcaacct cgcctacacc agagcgggag
     6301 tgctgctggc gctggcgttc gagacgctgc cgttcgtggt gcgagcggtc caaccggtgc
     6361 tgctcaccat ggagcccgac atggaggagg ccgcccgctg cctcggtgcc gggccgctgg
     6421 tgaccttccg gcggatcgtg ctgccgacca tcgccccggc gatgctggcc ggcgccgcgc
     6481 tcggcttcgc ccgggcggtc ggcgagttcg gctcggtggt gctgatctcc ggcaatctcc
     6541 cctacaagac cgagatggcc gcggtgcacg tgttcacgct ggtggaatcc gacgacccgg
     6601 ccggcgccgc cgccgtctcc gtcgtgctgc tggcgctgtc gctggtggtg ctggcggcga
     6661 tcagcctgct gcagcggaag gggacccgcc atgctgactg acgcgaccgc cacggccccg
     6721 accgcaccca gggccacggg gagccggcgc ggcagcaggg tcgcgctgcg gacggcggcg
     6781 ctcggctacc tcgcgctggt gctggtcgcg ccggtcggcg cgatcgtgtg gcgcaccttc
     6841 gcgcacgggc tggcgccggt gtggcgctcg gtcaccaccc ccgacgcgct ccacgccttc
     6901 tggctcaccg tgctggtcgc tgggatcgcg gtgccgctca acaccgtgtt cgggatcgcc
     6961 tgcgcggtgg tgctggtacg ccaccgtttc cccggcaagg ccctgctcaa cgccgtcatc
     7021 gacctgccgt tcgcgatctc gccggtggtg atcggcctcg cactggtgct ggtctacggc
     7081 caggacggct ggttcggtaa ctggctggtc acccatggcg tgcaggtgat cttcgcggtg
     7141 ccggggatgc tggtcgcgac cgtgttcgtg tcgttgccct acgtcgtccg cgaggtcacc
     7201 ccggtgctgc gcgaggtcgg caccgagcag gaggaggccg cctggacgct cggcgcgcgg
     7261 cccctgcggg cgttcctgcg ggtcaccctg ccggcgatcc gctggggtgt cgcctacggg
     7321 gtggtgctca ccaccgcccg cgcgatcggc gagttcggcg ccgtctcgat cgtgtctggc
     7381 cgcctcgccg gccagaccga gactctcacc ctgctggtcg aggaccgctt cgaacagttc
     7441 gacctggcag gcgcctacgc ggcctcgctg ctgctggccg cgctcgccct cctcactctc
     7501 ctggcgatga cccgactgcg gaaaaaggac ggtccctgat gtcgatcgag gcccgcaacc
     7561 tgaccaagcg cttcgggacc ttcaccgcgc tggacgacgt cagcgtgctg atcccgagcg
     7621 gttcgctgac cgccctgctg ggcccgagcg gcagcggcaa atcgacgctg ctgcgggtgg
     7681 tcgccgggct ggagcagccc gattccggcg cggtgctgat cgacggcgcc gacgcggcct
     7741 cggtcccggt gcagcgccgc gaggtcggct tcgtcttcca gcactacgcc gccttcaagc
     7801 acatgaccgt gcgcgagaac gtcgccttcg gcctgaagct gcgccgccgc ccgaaggcgg
     7861 aggtccgcgc ccgggtcgac cagctgctgg agctggtgca gatcgagggg ttcgccggcc
     7921 gctacccggc acagctgtcc ggcgggcagc gccagcgcat ggcgctcgcc cgggcgctgg
     7981 cggtggagcc gcgggtgctg ctgctggacg agccgttcgg ggcgctggac gcgcgcgtgc
     8041 gcaaggagct gcgcgcctgg ctgcggcgcc tccacgacga ggtgcacgtc accaccgtgc
     8101 tggtcaccca cgatcaggag gaggcgatgg aggtcgccga ccggatcgtg gtgatgaacc
     8161 acggccgcat cgagcagatc ggcgacccgc gctcgctcta cgagcagccc gccaacgact
     8221 tcgtgatgag cttcgtcggc ccggtcaccc gcctcggcgg ggacctcttg cggccccacg
     8281 acgtggcgct gctgccggcg ccagccgagg gcgcgcggga ggcgatgatc gaacggctgg
     8341 tccacctcgg cttcgaggtc cgcgccgagg tgatgctcgc cgacggtcag cgcctgctgg
     8401 tgcagctctc ccgcgaggag gccgagctgc tcgacctggc gcacggccag atcgtctacg
     8461 tccgccccac ccgcggccgc gccctcgcca gcgccgcccc ggccgccggg ccgaccggct
     8521 ggccctcggg taggggtagc ccgaaggcgg gtgtccggcc agcgtgatgg acggcggcta
     8581 gcgtggttcc tatcgtcgag gcgtgactga aagctgcctg cgataggacg atggaccatg
     8641 accaccccgg catcggtacc aaccgctccc gccccggcac catcgccgcg tccccgcccc
     8701 ggcgggtcgg cgctgcgccg tatggtgctg gccgtcgcga tcgtcgtcat ggtcgtgctg
     8761 ttcgcgttcg gcgcctggac cgccgtgggt tttgccacgc tgcgcagcgc cgagcggcac
     8821 cagagcttcg cgttcacggg ttcacggctg gtcgtcggca ccagcaatgg ctccatccac
     8881 gtcaccgcgg gtcaagccgg cagggtcgag gtggacaccc acctccgcta cagccaactc
     8941 aaccccccgc acccgactgt tcgggccgac ggcggccagc tcaccctcaa ggatggctgc
     9001 ccgaatgggg tggtcatctt ttgcgatgtc agcttcgacc tgcgcgtgcc cgctgcgctc
     9061 gcgctccagc tcaccaccag caacggagcc gtcgccgtca gtggcgtcac cggcgccctg
     9121 gacgtgcaca cctccaatgg cagcatcacc gccgacgggg ccaccggcca gctcagcctg
     9181 cggaccagca acggtgcgat cagggcgcgg gaccttcaca ccgcggtcgt cgatgccacc
     9241 accagcaacg gctcggtcac ggtctcgttc ctgaccactc ccacccatgt ggccgtcaac
     9301 accagcaacg gcgcggtgcg ggtcacggtc cccggtggca gcggccctta cagagtcgac
     9361 gtcaccaccg acaacggcag ccgaaccgtg gacgttccca ccgatccgaa cgcgacccgg
     9421 accatcagcg tccgcaccag taacggctcg gtgcacgttc tgcctgcagc ctcctaacga
     9481 ggtgcggcga atgttccatt ccgggacgaa ccacccaacc gatctttatg tgcgcttgcg
     9541 ctggggcggc gtatccttga gagcacgaag gggagtatcc cgactcggcc gcaccgtcat
     9601 cacggcatgc caggcggcat ctgcccggtg cgtcggccct gcgtcccgcg gggcgggaaa
     9661 gaccttcggc tgttgccctg caacaccgaa ggagtgtgca catgagccgg acccatcccg
     9721 cggcgatcag gatcgccgga ttcaaaatcg tggtcagctg gacgctgtcc atcgtggttg
     9781 cgctgatcgc ctggtcgctg gccacccagc tgctccccgc cgacttcccc ggccgtggcc
     9841 agaacgccta ctgggccgcc gccgcggtga ccacggcgct gttcctcgcc tccctgctcg
     9901 cccacgagct ggcccatgcg gtgctggccc gccaagtcgc cggcgtcgag gtcgaggaca
     9961 tcaccttctg gctgttcggg gggatgtcgc ggatgcgtgg cgagctgccc actccctgga
    10021 cgcagctgct ggtcaccgcc gccggcccgg ccgccagcct gctgctcggc ggcgccttct
    10081 ggctggcggc tgtggtgctg gccgcggtcg gcgccggcgg cctgccggcc ggcgcggcgc
    10141 tgtggctcgc tctgatgaac ctggcgctgg ccgtgttcaa cctgatcccg ggcgcgccgc
    10201 tggacggcgg ccggatgctg cacgcgctgc tgtggcgctt ccagggcagc cgtgcccgcg
    10261 gtgccctgac cgccgcccgc gtcggccagg gcaccgggat ggtgctggtc ggcctcggcc
    10321 tggtcgagct gctggtcatc cccggcaacg ccactggcgg gctgtggagc gcgctgatcg
    10381 gctggttcat catcggcgcc gcccgcaacg aggcgcgggt cgccaccgcg cggctgcggc
    10441 tcggcgagcg gcgcgtgcgc gagatcatga cccccgaccc ggcggtcggg cctggctggt
    10501 acaccgttga cgcgttcctc gacacgttcg cgtcgcggca ccgccaggcg gccttccccg
    10561 tgcgagactt cgacggccgg ctggtgggcg tggtcccgat gacgctgctc ggcgaggtgc
    10621 cgccggaacg gcgcggcctg ctgcgggtcc gcgacctcgc catcccgctg tcgtcgctgg
    10681 tggtcgccca cccagacgac ctggcggtcg agctggcgct gcgcctggct ggtgccaggc
    10741 agcgaagcgc gctggtggtg gaaggcgagc gggtggccgg cctggtctcc ccgtccaacc
    10801 tcgcggatcc gccgcgccgg gccgctggcc tggcggcggg gggcgccggg gcggggggcg
    10861 ccggagccgg tgccacgcca gcgccgctgc cgacctcggc cgagccgcgg tcggagcccc
    10921 agtgagcccg gcgctgaagg gcctggcgcg tggcgcgcgg cgggccggga tcctgatcgc
    10981 cgggctggcg ctggtcgcgg gcggcacggt cctgctggtg ttgcccggcc ctgggatcgc
    11041 cttgatcctt gccggactcg ccgtgctggc caccgagttt cactgggccc gccggctgct
    11101 cgcctgggct cgcgagcgtg cccgccaggt cgccgaccgg gcgcggcagc gcaccagaac
    11161 cagaaccaga accagaacca gaaccaggag cgggagcggg agcggctccg gccggccggg
    11221 ctagccgccg ggcccgttcc cgttccggtc ggctggcccg cgggcggcgt gccaggcggc
    11281 cagggcggcg gcgacggcga tcgcggtgac cgccgcgagc aggccgaggg cgagccgggc
    11341 gaccgcgccg accaggggca gctcggcggt gaccaggacc agcgcgccca gcgcggcggc
    11401 cagcgcggtg gtggccccga ggagggcgag ccgggcggcc agcaccggcg tctcgtcgcg
    11461 gaccagcggg tttgcttccc gggaccagtt ggccagctcg gcgagcagca ggtagtcggc
    11521 ggcgaacagc ggcgcccggc cgtcgaacgg accgcctccc tgccggtggt agagggagag
    11581 gccgtactca gcgaccagca ccgcgaccgc gaccggtgcc acgccagtga accgccacgc
    11641 cgccacggcc gccagcgcca ccccagccgc gccaagtccc agcagcggcc cggccgccac
    11701 ccgggcccca accagcggga ccagggcgag cgccagcgcc agcgccaccg cgccgccccc
    11761 agcggcgagg cggacgcggg cccgctgcgg tggccgctgc ggtggccgct gcggtggccg
    11821 gtccatccaa gcggtgggcg aggcgctcat ggcgaggcgc tcacggcgat ggcaccgagc
    11881 gggcggagct gcggcgccgg gacagctcaa gctcgtagac gacctgctcg gcggggacgc
    11941 cgggaggcca gtcgaccacc gggacgccga gttgctggta gcggtggcgc agcgcggcgc
    12001 gttccagcgc ccacaggcgt cgcgccagct cgtcaaggac cggaccggcg ccgctgcgcc
    12061 ggccgagggc gaggcggcgg cgggcatggc cgcgtcccgg agcggccgga cgatcgccgt
    12121 cacgggggac gagcggcgag acgtcgatga cggcgaggtc gtagccgcgg gcgagcacgt
    12181 cgaacagggc ggcggtggca cgctcatcga gcagcggggt gagcgcgagg atcagcgctt
    12241 tgggcggcag ggtgcggggc gggagctggt ggatgttacg ccagccgacg gtggcgaaga
    12301 tctgcgtctc catcagggtg tcgagcaggc ggtacagtgc ggcctggcca cttcccggct
    12361 gcagccagcg gaccacgcca ccgaagccga ccagcccgac ccggtcgcgg cgggcgaggt
    12421 aggcgctggc gagggaggcg gcggccccga ccgcacggtc cagcgtcccc cgctggaggc
    12481 ccggtggccc ggtggccgcc ggacccgtgc ccacgcccga ggcgggcacg tcggcgaagg
    12541 tgtccaggaa cagcaccacg tcggtgttgc gctcgggatg gcgctggttg acccaggggt
    12601 cgccgcggcg ggcggtggcg cgccagttga tagagcgggc ccggtcgccg ggctggaatg
    12661 gccgcacgtc ggcgaactcg ataccgtcgc cgcgctgccg cgcgacctgg ttgccggtga
    12721 acacctgcgt ctcgaagggg tgcaccagca tccgcagggt ctcgggctgc gggaagacga
    12781 ccagcgggag gctggtacgc accggccact cccagcgcag cagccgcagc gggtcgtggg
    12841 cgcgcagcag cacggtcccc agctggtagg cgccccagtg gtcacagcgc agccgcagcg
    12901 ccacagtccg gatctcgccg cggcccagcc gcagcgcccg ggcgggcagc tccacggcgg
    12961 cattggcaag ggcgagaccg tcggggacca ccagcagcgc ctccagccgt gacacgggcg
    13021 cggcggccac cagggtcagg gtgacgatga cttcgtcgcc ctcaacggcc cggtcggtgt
    13081 cggcctcggc ggtgaccgcg atctcgggcg tgcgggctgc cacctgcccg atgatcacca
    13141 gcagcgcgaa cggcgccgcg accgcgacca gttcgggctg cccggcggtg agcccggcga
    13201 gcagcagcag cgccgccagc ccgacgtagc cgccaagctt ggccgtcggc tgcctcatca
    13261 cggtcgcgcc ctcaccctca ccctcaccgc agcgccgggt cgcccggctc gggcatgccg
    13321 aatactcccc tccccgaagg gggtccccct gtttcaaatc tatccgaact ggcgcggggg
    13381 ctgccggggg tatccacagg gtcggggagc gccggcgggg tcggcacctg gtcgagcagc
    13441 tcggtcacca cgtcctcagt gcggacccgg cggacccaca gctcggggcg cagaatcagg
    13501 cggtgcgcca gggcgggcac cgcgaccgcc ttgacgtcct cgggggtgac gaagtcgcgt
    13561 ccggcgacgg cggcgttggc gcgggcggcc ttgaggaggg cgagggtgcc acgcgggctg
    13621 gcgccgacct gcacccgcgg gctggcccgg gtcgcgccga ccagctcgat caggtagcgt
    13681 ccgacgctct cgccgacgtg gacccgctcg atggcccgct gggcggcgag caggtcggcg
    13741 ggagtggcga ccgggtcgag gcggagctcg tcgctgcccc gctccagccg ccgggtgacc
    13801 atggcccatt cggcctccgg cgacgggtag ccgacggcca ggcgcagcag gaagcggtcg
    13861 agctgcgcct ccgggagcgg gtaggtccct tcgtactcga ccgggttctg agtcgcgagc
    13921 acgaggaacg gccgctcgag cgggtgcgtt gtcccttcga ttgtcacctg ctgctcctgc
    13981 atcccctcga gcagcgcagc ctg
//
LOCUS       DASWDC010000179         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_709862, whole genome shotgun sequence.
ACCESSION   DASWDC010000179 DASWDC010000000
VERSION     DASWDC010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709862"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..140
                     /locus_tag="VF995_05770"
                     /pseudo
     CDS             1..140
                     /locus_tag="VF995_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011908890.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta"
     gene            complement(369..1232)
                     /locus_tag="VF995_05775"
     CDS             complement(369..1232)
                     /locus_tag="VF995_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9343106.1"
                     /translation="MPRTGRPASRTVQSVDRGLTLLWMLAGAEGGLGVVEASERLGVD
                     PSTVSRLLASLVAADLVVRDPVSGRYAVGPGAARLAASVPASPSGADERELRQAARRT
                     LYLLEEGTGETVLLDVPSHGATHGPGMAVVVDVVPSRRVAPRLLLPAATRPLYATADG
                     KVLLAHALTETLERVLAAERPRLTPRTIGDPAVLRIQLTDIRRRGFSITEGEAQPGVN
                     VIAAPVQGPTGAVIAALAIEGPADRFDRIGMRRALDLLLPRATELSRRLGAPAPGGRQ
                     PTPGVDPFGFG"
     gene            1328..1861
                     /locus_tag="VF995_05780"
     CDS             1328..1861
                     /locus_tag="VF995_05780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009868035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9343107.1"
                     /translation="MLTEQPDSSTKAAGPEADAAAQISVEDFKRAAGSFTTGVTVVTT
                     RDPDGELRGFTANSFTSVSLDPPLVLVCLHRRAPSLGAFQAGCGFAVNVLADDQEWMS
                     QRFGRRSDNKFEGVPHQMGAAGAPVINGCIAHIECTLETSYDGGDHVILLGRVRNVAT
                     NDGSPLIFHRSRYLRAL"
     gene            1986..>3377
                     /locus_tag="VF995_05785"
     CDS             1986..>3377
                     /locus_tag="VF995_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX9343108.1"
                     /translation="MDQSFSRGAGAPGAVVAVDDRQLLKLMHWYDGFVVCLANPGFLI
                     ASLGASIGTLGALGAFVLWTVSMLIAVASNKIYTEPATMFPDRAGGIAVYAHEGWRRH
                     FTFVGPMAAVGYWAAWSVVLAIFGITIGSLIQAQWFPNALWTVHLGLGGHGVALTLPR
                     IIAVIAIFAVWAFNASGVRPAVWVGYVTGALLMIPLAVFIVLPYLTGDFHRSNLTWLV
                     SSGGSLWNSWRLALVWLYLMGWSAYGVEGAATFAPEYKDTTRDTALALRRSAAFSLIV
                     YAFLPIGVGGVLPADQIAKGALDYSFYVVEMDKLIGAGANFVLPLLIASFILAMNTAT
                     MDGSRALYGISKSGMTIKWLSHLNRFHVPSRAMTVDMVFNIAVVLLLNSTIAILAASN
                     LGYILCHVFALSAVLLLRRDRPEWPRPLPLSRFWLWVAGVLALANLLFVVVGAPSFKL
                     TGYGSYKELLIGIG"
BASE COUNT          461 a         1217 c         1144 g          555 t
ORIGIN      
        1 tcggcccgga gcggatcaag cggctggtcc ccttcatgga cgtctccgag caggcccgct
       61 acccgatcct cggcgccctg taccacccgc ccgggggcac gatccgccac gacgcggtca
      121 actggggcta cgcccgcgcc gacgatcacc acgtcgtagc tgggggcgag ctcgtgctga
      181 tggaagctgc gcggccagtc ggcatgcgac agcccgcgcc ggatgagctg gagggcggag
      241 tagcgcgcgg gcttccgctg cgatttcggc tggccgttgc gaccgttcat ggtgtctcgg
      301 aacatatcgc gagaaggaaa cgctgtccac tggcagtaaa caagaatttc atcatgccgt
      361 gaggtctcct aaccgaagcc gaacgggtcc acgccggggg ttggctggcg gccgccggga
      421 gcgggcgcgc cgaggcggcg gctcagctcg gtggcgcgcg gcagcagcag atccagggcg
      481 cggcgcatgc cgatgcggtc gaagcggtca gccggtccct cgatcgccag ggcggcgatc
      541 acggcgccgg tcggtccctg gacgggcgcg gcgatcacat tgacgcccgg ctgcgcctcg
      601 ccctcggtga tggagaagcc gcggcgccgg atgtcggtga gctggatgcg cagcaccgcc
      661 gggtcgccga tggtgcgcgg ggtcagtctg ggccgctcgg cggcgagcac ccgctccagc
      721 gtctcagtga gcgcgtgggc gagcagcacc ttgccgtcgg cggtggcata cagcggccgg
      781 gtcgcggcgg gcagcagcag ccgtggcgcc acgcgccggc tcggcaccac gtccaccacc
      841 accgccatcc ccgggccgtg cgtcgcgccg tggctgggca cgtccagcag gacggtctcg
      901 ccggtgccct cctcgagcag gtacagggtg cgccgcgcgg cctggcgcag ctcccgctcg
      961 tcggcgccgg aggggctggc gggcaccgag gcggccaggc gggccgctcc cggcccgacc
     1021 gcgtagcggc cgctgacggg gtcgcgcacc accaggtcgg cggcgaccag cgaggcgagc
     1081 aggcgcgaga cggtgctcgg gtcgacgccg agccgctcgg acgcctccac cacgccgagg
     1141 ccgccctcgg cgccggccag catccacagc agggtgaggc cgcggtcgac cgactggacc
     1201 gtgcggctgg cgggacgccc cgtccgtggc aaccgcccct tgcctcctaa ccgtcccgtg
     1261 ggctagtctt tgcgtggttc ccaattggta ttgcgtcttt ggcaatataa cacgggaggt
     1321 tgggcaattg ctcaccgagc aacccgactc gagcaccaag gccgcgggcc cggaagccga
     1381 cgccgccgcc cagatcagcg tcgaggactt caagcgcgcc gccggttcgt tcaccaccgg
     1441 cgtcaccgtg gtcaccaccc gtgaccccga cggggagctg cgcggcttca ccgccaactc
     1501 gttcacctcg gtgtcgctcg accccccgct ggtgctggtc tgcctgcacc ggcgcgcccc
     1561 gagcctgggt gccttccagg ccggctgcgg cttcgccgtc aacgtcctcg ccgacgacca
     1621 ggagtggatg tcacagcgct tcgggcgccg ctccgacaac aagttcgagg gcgtccccca
     1681 tcagatgggc gccgccgggg cgccggtgat caacggctgc atcgcccata ttgagtgcac
     1741 cctggagact agctatgacg gaggggatca tgtcatcctc ctcggccggg tgcgcaatgt
     1801 ggcgacaaac gacggatcgc cgctgatctt ccaccgaagc cggtacctgc gcgccctgta
     1861 ggagccagcg aggaggagcc ccctttgccc cagcacagca ccacccgctc gccaccaccg
     1921 cgatagcccc cgcacaaccc tcgtcccccc catcctcatc tctcatgcga gcgggagaag
     1981 accctatgga ccagtctttc tcgcgcgggg ccggtgcgcc aggagccgtc gtcgcggtcg
     2041 acgaccgtca gctcctgaag ctcatgcact ggtacgacgg ctttgtcgtc tgcctcgcca
     2101 accccggctt cctgatcgcc agcctcggcg cctccatcgg gaccttgggc gccctcggcg
     2161 cgttcgtgct atggaccgtc tcgatgctga tcgcggtcgc ctcgaacaag atctacaccg
     2221 agccggccac catgttcccc gaccgggccg gcggcatcgc cgtctacgcg cacgagggct
     2281 ggcgccgcca cttcaccttt gtcgggccga tggctgcggt gggctactgg gccgcctggt
     2341 cggtggtgct cgcgatcttc gggatcacca tcgggtccct gatccaggcg cagtggttcc
     2401 cgaacgcgct gtggacggtg cacctcggcc tcggaggcca cggcgtggcg ctgacgctcc
     2461 cgcggatcat cgccgtgatc gcgatcttcg ccgtgtgggc cttcaacgcc tctggggtgc
     2521 ggccggcggt ctgggtcggc tatgtgaccg gcgcgctgct gatgatcccg ctggcggtgt
     2581 tcatcgtcct tccctacctc actggcgact tccaccggag caacctcacc tggctggtct
     2641 ccagcggcgg ttcgctgtgg aacagctggc gtctcgcgct ggtgtggctc tatctgatgg
     2701 gctggtcggc gtacggggtc gagggggcgg cgacgttcgc gcccgagtac aaggacacca
     2761 ctcgtgacac cgcgctcgcc ctgcggcgct cggcggcctt ctccctgatc gtctacgcgt
     2821 tcctgccgat cggggtcggc ggcgtgcttc ccgccgacca gatcgccaag ggcgccctcg
     2881 actactcctt ctacgtcgtc gagatggaca agctcatcgg cgccggcgcg aacttcgtgc
     2941 tgccgctgct gatcgcgtcg ttcatcctgg cgatgaacac cgccacgatg gacggctccc
     3001 gcgccctcta cggcatctcc aagtcgggca tgaccatcaa gtggctgtca cacctcaacc
     3061 gcttccacgt gccatcccgt gcgatgaccg tggacatggt gttcaacatc gcggtcgtgc
     3121 tgctgctcaa ctcgaccatc gcgatcctgg ccgcgagcaa cctcggctac atcctctgcc
     3181 acgtgttcgc gctctcggcg gtgctgctgc tgcgccgcga ccggccggag tggccacggc
     3241 cgctgccgct gtcgcggttc tggctctggg tcgcgggtgt cctggcgctg gccaacctgc
     3301 tgttcgtggt ggtgggggcg ccctccttca agctcaccgg ctacggctcc tacaaggagc
     3361 tgctgatcgg catcggc
//
LOCUS       DASWDC010000180         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_439820, whole genome shotgun sequence.
ACCESSION   DASWDC010000180 DASWDC010000000
VERSION     DASWDC010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439820"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1299
                     /locus_tag="VF995_05790"
     CDS             352..1299
                     /locus_tag="VF995_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9343109.1"
                     /translation="MTAWLLSAIDWDLGPATSLVLLGGGLGLTLFAGGALAATHPWRG
                     PRPNEEIAAEIAHSPAPPLVRLRSFLRARVDPKEATGLALTVALVCIWLAAIWFGALA
                     DMVASHTGLARWDAAAANWGAAHATHTSTDVLIGLTDLGSTLVVIAVALLGGLYERLR
                     WGRWNALIFMIVVVWGQNLIANGVKLLVHRQRPAVPHLIHATGWSFPSGHAAASAATY
                     AALALLLGRGRSWPVKAWLGTAAAAVAVAVASSRVLLGVHWLTDVFAGSALGFGWFAV
                     CSIAFGGGILRFGATAERAAAQAEAEAAPPAPGGDRPGP"
     gene            1342..2187
                     /locus_tag="VF995_05795"
     CDS             1342..2187
                     /locus_tag="VF995_05795"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX9343110.1"
                     /translation="MTITALTSAEISLPARPDASEVRERIAVLAVHVDLVGLTDNHAG
                     MPRMSPLAAVALAREQGVATVVHVSCRDRNRLALRSEVIGAAALGSEGILCLYGDPVP
                     GVPRVKDYTTTKLLSEARSWVEPYNLALGCVADPFAVDVEGELALLGRKVAADADFLQ
                     TQMVFEPDRLVAFLEQAREGGKLDGCRVYASVPVVRTARMADVLNRALPFDLPDRAYR
                     QIAAGGGLDLATETALTLARQPLVDGLHVMAWGREADAGQVAAAFRRARGAPAERLPG
                     TTNGA"
     gene            2184..>3335
                     /locus_tag="VF995_05800"
     CDS             2184..>3335
                     /locus_tag="VF995_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343111.1"
                     /translation="MKTAPSRSAQIERRPRRQAAVATAATALVLVAVGVLGTFGHRGW
                     GQVPFSVPSIPWYFSWPYYVFPVPGFDARWGFAALLAACAAAATVLALARAPALLPIL
                     RRRVPRLPRPVWLAMALLLAFVLDLAVAAIRGGPAAWAEPISHPGEYAPAAGLVGPIP
                     DFLRHFAERAPLIGGFVAQHPPGAVIFYSLVARVCSGLAACALATVACACLGLLVTAA
                     LARDELHGRDDDGDGEGPGGSGGMVPPPEGPRADSMVAAGPPDATALAVAFWVLCPAV
                     ILYSATSADAMWAPLLAGAALASHRGLLRRSLRWTLAGGALLWLASMATYAAVLVVPF
                     LLVRALAVARSEGNQHQHQDQVEAERGEAGDGVRGGWLWAWPWAARWAAL"
BASE COUNT          339 a         1318 c         1188 g          490 t
ORIGIN      
        1 agccagccgg ccaggatgcc gaccagcagg atcagcaccg cccagccgaa caggtacagc
       61 gcggatggga ccccggcgcg ggcgtgccgc cagcgctgcg ccagcgccac cccgcgcggg
      121 cccggcggtc gctccagcgg cccgtccggc tgtggtgtgg tcaacatcgg gacctccggc
      181 ggtcagtcca gtcagtccag tctggttctg ggactggtcc cgggtggcga accgcccggg
      241 tggtcgagcc cggtatccct ccacaggagg cgagcaaacc tttggcgcct gcgggaaccc
      301 cgggacgcct gctacggttt ggcgttcatc gtcgtgccta tcctgatacc catgactgcc
      361 tggctgctct ccgcgatcga ctgggacctc gggcccgcga ccagcctcgt gctgctcggc
      421 ggcgggctcg ggctcacgct gttcgcgggc ggtgcgctgg ccgccacgca cccctggcgc
      481 ggcccccggc cgaacgagga gatcgccgcg gagatcgcgc actcgcccgc gccgccgctg
      541 gtgcggctgc ggtccttcct gcgcgcccgc gtcgacccga aggaggcgac cgggctcgcc
      601 ctcaccgtgg cgctggtgtg catctggctg gcggcgatct ggttcggcgc gctcgccgac
      661 atggtcgcct cccacaccgg gctggcccgc tgggacgccg ccgcggccaa ctggggtgcc
      721 gcgcacgcca cccatacctc caccgacgtg ctgatcggcc tcaccgacct cggcagcacc
      781 ctggtggtga tcgccgtggc gctgctcggc ggcctctacg agcggctccg ctggggccgc
      841 tggaacgcgc tgatcttcat gatcgtggtc gtctggggcc agaacctgat cgccaacggc
      901 gtcaagctgc tggtccaccg ccagcggccc gccgtgccgc acctgatcca cgcgaccggc
      961 tggtcgttcc cgtccgggca cgccgccgcc tcggccgcga cctacgccgc tctcgcgctg
     1021 ctgctcgggc gcggccggag ctggccggtc aaggcctggc tcggcaccgc cgccgccgcg
     1081 gtcgcggtgg cggtcgccag ctcccgggtg ctgctcgggg tccactggct caccgacgtg
     1141 ttcgccggct cggcgctcgg cttcggctgg ttcgcggtct gctcgatcgc cttcggcggc
     1201 ggcatcctcc gcttcggcgc caccgcggag cgcgccgccg cccaggccga ggccgaggcc
     1261 gcgccgccag ctcccggcgg agaccgcccc gggccgtgac tgacgcaatt ccggcagcgt
     1321 gattcgggta gccttctctc catgaccatc acagcgctca cctccgccga gatctcgctg
     1381 ccggcccggc cggacgccag cgaggtccgt gaacgcatcg cggtgctcgc ggtgcacgtc
     1441 gacctggtcg gcctgaccga caaccacgcc ggcatgccgc gcatgtcgcc gctcgccgcc
     1501 gtcgccctcg cccgggagca gggggtggcc acggtggtgc acgtctcctg ccgcgaccgc
     1561 aaccggctgg cgctgcgctc cgaggtgatc ggcgccgccg cgctcgggtc cgagggcatc
     1621 ctctgcctct acggcgaccc ggtgccgggc gtcccccggg tcaaggacta caccaccacc
     1681 aagctgctgt cggaggcccg gtcctgggtg gagccctaca acctggcgct cggctgcgtg
     1741 gccgacccgt tcgcggtcga cgtcgagggc gaactggccc tgctcggccg caaggtcgcc
     1801 gccgacgccg acttcctgca gacccagatg gtgttcgagc ccgaccgcct ggtggccttc
     1861 ctggagcagg cgcgagaggg cggcaagctc gacggctgcc gcgtctacgc cagcgtcccg
     1921 gtggtccgca ccgcccgcat ggccgacgtg ctgaaccgcg ccctcccctt cgacctgccc
     1981 gaccgcgcct accgccagat cgccgccggc ggcggcctcg acctggccac cgagaccgcc
     2041 ctcaccctcg cccggcagcc gctggtcgac ggcctccacg tcatggcatg gggcagggag
     2101 gccgacgccg gccaggtcgc cgccgcgttc cgccgcgcac gcggcgctcc agccgaacgc
     2161 ctcccgggta cgacgaacgg cgcgtgaaga ccgcaccttc aagatcagcc cagattgagc
     2221 gccggccgcg gcgccaggcc gccgtcgcca cggcggccac cgccctcgtg ctggtcgcgg
     2281 tcggcgtgct cggcaccttc ggccaccgtg gctggggtca ggtgcccttc agcgtgccca
     2341 gcatcccgtg gtatttcagc tggccctact acgtcttccc ggtgcccggc ttcgacgccc
     2401 gctgggggtt cgccgcgctg ctggccgcct gcgccgccgc cgccacggtc ctggcgctcg
     2461 cccgcgcccc ggccctcctc ccgatcctgc gccgccgcgt gccccggctg ccgcgcccgg
     2521 tctggctcgc catggcgctg ctgctcgcct tcgtgctcga cctggcggtg gccgcgatcc
     2581 gcggcgggcc ggcggcctgg gccgagccga tttcccatcc cggcgagtac gcgcccgccg
     2641 ccggcctggt cgggccgatc ccggacttcc tccgccactt cgccgagcgg gcgccgctga
     2701 tcggcgggtt cgtcgctcag cacccgccgg gtgcggtgat cttctactcg ctggtcgcgc
     2761 gggtctgctc cgggctcgcc gcctgcgcgc tcgccacggt ggcctgcgcc tgcctcggcc
     2821 tgctggtcac cgccgccctg gcgcgcgacg aactccacgg ccgtgacgac gacggggacg
     2881 gggaggggcc ggggggttcg gggggcatgg tcccgccgcc ggagggcccg cgagcggatt
     2941 ccatggttgc cgcggggccc cccgatgcga cagccctggc ggtcgcgttc tgggtgctgt
     3001 gcccggcggt gatcctctac tcggcgacct cggccgacgc gatgtgggcg ccgctgctgg
     3061 ccggcgccgc gctcgcgtcc caccgcggcc tcctccggcg ctcgctccgc tggacgctcg
     3121 cgggcggcgc gctgctgtgg ctcgcctcga tggccaccta cgcggccgtg ctggtggtcc
     3181 cgttcctgct ggtccgggcc ctcgccgtcg cccgctccga gggcaaccag catcagcatc
     3241 aggatcaggt ggaggccgag cggggggagg ccggcgacgg ggttcggggt ggctggctat
     3301 gggcctggcc atgggcggcg cgctgggcgg cgctg
//
LOCUS       DASWDC010000181         5385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_356843, whole genome shotgun sequence.
ACCESSION   DASWDC010000181 DASWDC010000000
VERSION     DASWDC010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356843"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..977
                     /locus_tag="VF995_05805"
     CDS             66..977
                     /locus_tag="VF995_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX9343112.1"
                     /translation="MLRATLLKAAGSTTLRRQMTSNPVARRVAHRFVAGDTLDDAERV
                     IRVLNADGIAVALDYLGENTESEGQARASTASYLAALDRIQDQGLNANISVKLTALGL
                     DIGEQLATEEAAKVAARGKEVGAMVGVDMEAQPYVERTLDLAERLQDSYDGVGVCVQA
                     YLYRSGEDLARLNRGGVPVRLVKGAYQEPPEVAWPDKAQVDDAYARLLESLLAANPYP
                     MVATHDPRLVRLTKTLAAKLGRDRSSFEFQMLYGVRRDLQQQVVAEGYRLRVYVPYGD
                     QWYPYFMRRLAERPANLSFFLSNLVKK"
     gene            1029..2495
                     /locus_tag="VF995_05810"
     CDS             1029..2495
                     /locus_tag="VF995_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9343113.1"
                     /translation="MDRSAAHNFRNGIASGLAEGVASGSAAVVVALALLLALAGCSSG
                     ADKRFAAAVVRPATVVERVSAPGAVQAAAVADLVAPATAKIQTLRVADGATVRHGQVL
                     AQLSSAQVDASIRQAQAALGSASSLGGIIPSLPTGAALAALDQLQQQVSSSSGAVLDA
                     LRATLPLLPKAQRAAVQARLDQAAQRVATAQQAAAAAARQAASAVNQQTAALRDAIGA
                     ATAAQRGQAQLALDLANQQKAALTLRAPLAGTVQLGRSAAGSGSSGGVAGLPSISSLP
                     ESAQQALQSLAGASGGSGSGSGSSGPPLRAGSQVSAGQAVATVYDVSTLMVAAEVDET
                     DIPLVKVGQEADVELDAFPGASFFARVKRISVAPTQGQSAAGGAAYEVDIAIGRVEQA
                     GSGDASPVPRVGMTATAEIHVRRAAEAPAVPASAMIEQGRGQAVYIIEGGRVHLRTIT
                     VAAEGEDQVAVASGVRNGERVVSRGAERLHDGQTWHGS"
     gene            2589..3269
                     /locus_tag="VF995_05815"
     CDS             2589..3269
                     /locus_tag="VF995_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9343114.1"
                     /translation="MVEAGGVTKVYRLDGVEVRALDGIDLTVAGGDSLAIMGPSGSGK
                     STLLGLLGGLDRPTSGTLAFGGRDVAALSDDELARLRNQVVGFVFQNFQLLPRTSALG
                     NVGLPLVYRGVGRAERRRRATEALEAVGLGHRLQHRPSQLSGGEQQRVAIARALVTEP
                     AMLLADEPTGNLDSASGAEVLDLLGSLHAERGVAVVAVTHDAAVAARFGRIVVVRDGR
                     VASTSREG"
     gene            3272..4456
                     /locus_tag="VF995_05820"
     CDS             3272..4456
                     /locus_tag="VF995_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9343115.1"
                     /translation="MRLGEALRVAWESLRANKLRSGLTMLGVVIGVTSVVLLVAIGSG
                     ARNEVTSGVESLGSNILFVAPGNFSFGSTPAVSRLTLEDERRVADAIGDPRRVTVTVA
                     SGELAWVGARRTTVNLNGTNEAIPTVFDRTVERGSFFSAADVEGRRRVAVLGADPAEA
                     LFPDRDPLGREVTIGGLRFRVVGVLSRVGTALGVDRNAETFVPVTVAQRMLGVNRVDA
                     IAVKAPSTGDIAPEQALIRSVVSRAHPEQSYQVLTQEDILGVVGRILNVLTLVLASIA
                     GISLLVGGVGVMNIMLVSVSERTREVGLRKAVGARTRDVLRQFLLEAVALTVSGGVVG
                     IVLGIAGALLVARLSPVPAAITGWSVALAFGVSVAVGIFFGVYPARKAARMQPVAALR
                     YE"
     gene            4558..4983
                     /locus_tag="VF995_05825"
     CDS             4558..4983
                     /locus_tag="VF995_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018542988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX9343116.1"
                     /translation="MPLMSKGQWRAFLTERPRTGKLAVVRADGAPHVTPVWFDLDGDE
                     IVFTTHETGIKGRSILRDPRVCLSVDDDAPPFAFVIINGTARVSTDPDDLRYWAARLA
                     GRYMGADRAEEFGRRNGVPGELVVRVKPTKVVAADKVAD"
     gene            complement(5002..>5385)
                     /locus_tag="VF995_05830"
     CDS             complement(5002..>5385)
                     /locus_tag="VF995_05830"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX9343117.1"
                     /translation="AAGIQTVLEDRLHLAGIPATGLWARVPPYLAGGTHPPAALALVR
                     TVGRLAGVEIDTSELEAASKDHLEQVEAAIRERPQIAEFIDQIRGLIEEGADQRIPSG
                     DEIAAELERFLSQQPPDEGGDPWPR"
BASE COUNT          689 a         1979 c         1989 g          728 t
ORIGIN      
        1 cggcttgccg aggaatgcga tactgcgagt gagccaacgg aggttctctc cctgggaggt
       61 ggggcgtgct cagggcaacg ctgctgaagg ccgccgggtc gacgaccctg cggcggcaga
      121 tgaccagcaa tccggttgcc cgccgggtgg cgcaccggtt cgtcgccggc gacacgctgg
      181 acgacgccga gcgggtgatc agggtcctga acgcggacgg gatcgcggtc gcgctcgact
      241 acctgggcga gaacaccgag tcggaggggc aggcgcgcgc ctcgaccgcg tcctacctgg
      301 ccgccctcga ccggatccag gaccaaggcc tgaatgccaa tatctcggtc aaactgaccg
      361 cgctcgggct cgatatcggc gagcagctcg ccaccgagga agccgccaag gtggcggcac
      421 gcggcaagga ggtcggcgcc atggtcggcg tcgacatgga ggcccagccc tacgtcgagc
      481 gcaccctgga cctggccgag cggctccagg actcctacga cggcgtcggc gtctgcgtgc
      541 aggcctacct gtaccgcagc ggcgaggatc tggcacggct gaaccgcggc ggggtgccgg
      601 tccgcctggt caagggcgcc taccaggagc cgcccgaggt cgcctggcca gacaaggccc
      661 aggtcgacga cgcctatgcc cgcctgctgg agtcgttgct ggccgccaac ccctacccga
      721 tggtcgccac ccacgacccc aggctggtcc gcctcaccaa gacgctggcc gccaagctcg
      781 gccgcgaccg atcgagcttc gagttccaga tgctctacgg cgtccgccgc gacctccagc
      841 agcaggtggt ggccgagggc taccgcctgc gcgtctacgt cccctacggc gaccagtggt
      901 acccgtattt catgcgccgt ctcgcggagc ggcccgccaa cctctccttc ttcctctcca
      961 acctggtcaa gaagtgacct tcgctgacac gctccgcgta accgcagggt cgccactatg
     1021 ctggccatgt ggatcggtca gcagcgcaca acttcaggaa cggtatcgcc agcggtctcg
     1081 ccgagggcgt cgccagcggc agcgccgcgg tggtcgtggc cttggccctc ttgctggcgc
     1141 tcgctgggtg cagctcagga gctgacaagc ggttcgccgc cgcggtggtg cggcccgcca
     1201 ccgtggtcga gcgggtcagc gcccctggcg ccgtgcaggc cgcggcggtg gcggacctgg
     1261 tcgcgccggc caccgccaag atccagaccc tgcgggtggc cgacggcgcc accgtccgcc
     1321 acgggcaggt gctggcccag ctctcctcgg cccaggtcga cgcgtcgatc cggcaggcgc
     1381 aggcggcgct cggctccgcg tcgtcgctcg gcgggatcat cccgagcctg ccgacgggcg
     1441 cggcgctggc cgccctcgac cagctccagc agcaggtctc cagcagcagc ggagccgtcc
     1501 tcgacgccct gcgggccacc ctaccgctgc tgccgaaggc gcagcgggcg gccgtccagg
     1561 cccggctcga ccaggccgcc cagcgggtcg ccaccgccca gcaggcggcc gccgcggcgg
     1621 cgcggcaggc tgccagcgcc gtcaaccagc agaccgcggc gctgcgcgac gccatcggtg
     1681 ccgccaccgc cgcgcagcgc ggccaggcgc agctcgccct cgacctggcc aaccagcaga
     1741 aggccgcgct gacgctgcgg gcgccgctgg cgggcacggt gcagctcggc cgctccgccg
     1801 ctggctccgg ctcgtcgggg ggcgtcgcgg gcctgccgag catctcctcc ctgccagaga
     1861 gcgcccagca ggccctccag agcctcgcgg gtgcgagcgg cggcagcggg agcggcagcg
     1921 ggtcgagcgg cccgccgctg cgggccggct cgcaggtcag cgccggccag gccgtcgcga
     1981 ccgtctacga cgtcagcacc ctgatggtcg ccgccgaggt cgacgagacc gacatccccc
     2041 tggtcaaggt cggccaggag gccgatgtcg agctggacgc cttccccgga gcgagcttct
     2101 tcgccagggt gaagcgcatc tcggtggccc cgacccaggg ccagtcggcc gccggcgggg
     2161 cggcctacga ggtcgacatc gccatcgggc gggtcgagca ggccggctcg ggggatgcca
     2221 gcccggtgcc tcgggtcggc atgaccgcca ccgccgagat ccacgtgcgc cgcgccgcgg
     2281 aggcacccgc ggtacccgcc tccgccatga tcgagcaggg ccgcggccag gcggtctaca
     2341 tcatcgaggg cgggcgggtg cacctgcgca ccatcaccgt cgccgccgag ggggaggacc
     2401 aggtcgccgt cgccagcggc gtccgcaacg gcgagcgggt ggtgtcgcgc ggcgccgaac
     2461 ggctccacga cggccagacc tggcacggga gctgacgcgc gatggcgctg gtgcggtcgc
     2521 ggtcgcgctc ccggcacgcc acctcagtcc cggaggcggg cgtggtcgca gccggggagg
     2581 gccgggcggt ggtggaggcc ggcggcgtga ccaaggtcta ccgcctcgac ggcgtcgagg
     2641 tccgcgccct ggacgggatc gacctgaccg tggccggtgg cgactcgctg gcgatcatgg
     2701 gcccgagcgg gtcgggcaag tcgaccctgc tcggcctgct cggcggcctc gaccgcccga
     2761 ccagcggcac gctggccttt ggcggccgcg acgtcgccgc cctctccgac gacgagctgg
     2821 cgaggctgcg caaccaggtg gtcgggttcg tgttccagaa cttccagctg ctgccgcgta
     2881 cctccgcgct cggcaacgtc ggcctcccgc tggtctaccg gggcgtcggc cgcgccgaac
     2941 gccgccgccg tgccaccgag gcgctggagg cggtcggcct cggccaccgc ctgcagcacc
     3001 gcccctcgca gctctccggc ggcgagcagc agcgggtcgc gatcgccagg gcgctggtca
     3061 ccgagccggc catgctgctc gccgacgaac ccaccggcaa cctcgactcc gcctccggcg
     3121 cggaggtcct cgacctgctc ggcagcctcc acgccgaacg cggcgtggcg gtcgtggcgg
     3181 tgacccacga cgccgcggtc gcggcccgct tcggccgcat cgtggtggtc cgcgacggcc
     3241 gcgtggcctc aacctcaagg gaaggttgag ggtgcggctc ggcgaggcgc tgcgggtcgc
     3301 gtgggagtcg ctgcgcgcca acaagctccg ctccgggctc accatgctcg gggtggtcat
     3361 cggggtcacc agcgtcgtgc tgctggtggc gatcggcagc ggggcccgca acgaggtgac
     3421 ctccggggtg gagtcgctcg gctccaacat cctgttcgtg gctcccggca acttctcgtt
     3481 cgggtcgacc ccggcggtgt cgcgtctgac cctggaggac gagcggcggg tcgccgacgc
     3541 gatcggcgac ccgcggcggg tgacggtgac ggtcgcctcg ggcgagctgg cctgggtcgg
     3601 cgcccgccgc accaccgtca atctcaacgg caccaacgag gcgatcccca ccgtgttcga
     3661 ccggacggtc gagcgcggca gcttcttcag tgccgccgac gtggagggcc gccgccgggt
     3721 cgccgtgctc ggcgccgacc cggccgaggc gctgttcccc gaccgcgacc cgctcggccg
     3781 cgaggtcacc atcggcggcc tgcgcttccg cgtcgtcggc gtgctcagcc gggtcggcac
     3841 cgcgcttggg gtcgaccgca acgccgagac cttcgtgccg gtcacggtcg cgcagcgcat
     3901 gctcggcgtg aaccgggtcg acgccatcgc cgtcaaggct ccctccaccg gcgacatcgc
     3961 ccccgagcag gccctcatca ggtcggtggt ctcccgcgcc cacccggagc agtcctacca
     4021 ggtgctgacc caggaggaca ttctcggggt ggtcggccgc atcctcaacg tcctcaccct
     4081 ggtgctcgcc tcgatcgctg ggatctcgct gctggtcggc ggggtcgggg tgatgaacat
     4141 catgctggtg agcgtgtccg agcggacccg cgaggtcggc ctgcgcaagg ccgtcggtgc
     4201 gcgcacccgc gacgtgctgc gccagttcct gctggaggcg gtcgccctca ccgtcagcgg
     4261 cggggtcgtc gggatcgtgc tggggatcgc gggcgcgctg ctggtggcga ggctgtcgcc
     4321 ggtgccagcg gcgatcaccg gctggtcggt ggcgctcgcg ttcggggtca gcgtcgccgt
     4381 ggggatcttc ttcggggtct acccggcccg caaggccgcc cggatgcagc cggtcgccgc
     4441 cctgcgctac gagtaggggg cctccgcccc ggctccagct tccgggactg gcgcggtgcg
     4501 gcgggatagg ctggcgtgtg gcgtgatcac cccaggagag tctcaggagg ttggcggatg
     4561 ccgctgatgt caaaggggca gtggcgggcg ttcctgacgg agcggccgcg cacagggaag
     4621 ctcgcggtgg tgcgagccga cggggcgccg cacgtcaccc cggtctggtt cgacctggac
     4681 ggcgacgaga tcgtcttcac cacccacgag accgggatca agggcaggag catcctccgc
     4741 gacccccgcg tctgcctgag cgtcgacgac gacgccccgc ccttcgcctt cgtgatcatc
     4801 aacggcaccg ctcgcgtctc caccgatccc gacgacctgc gctactgggc ggcccggctc
     4861 gccggccgct acatgggcgc cgaccgtgcc gaggagttcg gccgccgcaa cggcgtcccg
     4921 ggcgagctgg tggtccgcgt caagccgacc aaggtcgtcg cggcggacaa ggtcgcggac
     4981 tgagggcgcg gaggcgagcc ctcagcgggg ccaggggtcg ccgccctcgt cgggtggctg
     5041 ctggctgagg aagcgctcca gctcggcggc gatctcgtca ccggagggga tgcgctggtc
     5101 ggcgccctcc tcgatcaggc cgcggatctg gtcgatgaac tcggcgatct ggggccgctc
     5161 gcggatggcc gcctcgacct gctccaggtg gtccttggag gcggcctcca gctcggaggt
     5221 gtcgatctcg accccggcga gacgcccgac cgtacgcacc agcgccagcg cggccggtgg
     5281 gtgcgtcccg cccgccaggt acggcggcac ccgggcccac aggccggtgg cggggatgcc
     5341 ggccaggtgg aggcggtcct cgagcaccgt ctggatgccg gcggc
//
LOCUS       DASWDC010000182         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1296623, whole genome shotgun sequence.
ACCESSION   DASWDC010000182 DASWDC010000000
VERSION     DASWDC010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296623"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VF995_05835"
     CDS             <1..592
                     /locus_tag="VF995_05835"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9343118.1"
                     /translation="MVGVEIATTVDGAELDDPGLYPFWRTAAELGCLVLIHPYQALAG
                     RRVVRYFLGNLVGNPAESSIAVAHLIFGGVLERFGDLRVCVVHGGGFLPWQAGRLDRG
                     FAAKPEVCAPKLTRPPSEWLRHLYYDTILHSPAALAWLVAFAGADHVLLGSDYPFEMR
                     DPTPVATVNAIPGLTDEQRALILEGNLLRLLGHLIK"
     gene            765..1100
                     /locus_tag="VF995_05840"
                     /pseudo
     CDS             765..1100
                     /locus_tag="VF995_05840"
                     /inference="COORDINATES: protein motif:HMM:NF016550.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            1216..1599
                     /locus_tag="VF995_05845"
     CDS             1216..1599
                     /locus_tag="VF995_05845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9343119.1"
                     /translation="MRGAAQTLDFAQPVALLLIALLHLLPDADDPAGIVARLLAPLAP
                     GSYLALQHLGLDANEPKALAVSAAAGRDGVTLLPRRRTEILRFFDGLELVEPGLVQDA
                     LWRPDGEVPAEFRRSWGFAGVARKS"
BASE COUNT          260 a          750 c          609 g          296 t
ORIGIN      
        1 catggtcggg gtcgagatcg ccaccaccgt ggacggcgcc gaactcgacg accccggcct
       61 gtacccgttc tggcgtaccg ccgctgagct cggctgcctg gtgctgatcc acccctacca
      121 ggccctcgcc ggccgccgcg tggtgcgcta cttcctcggc aacctggtgg gcaaccccgc
      181 cgagagcagc atcgccgtcg cccacctgat cttcggcggc gtcctggaac gcttcggcga
      241 cctgcgcgtc tgcgtcgtcc acggcggcgg cttcctcccg tggcaggccg gccgcctcga
      301 ccgcggcttc gccgccaaac cagaggtgtg cgcccccaag ctcacccgcc cccccagcga
      361 gtggctgcgc cacctctact acgacaccat cctccacagc cccgccgccc tcgcctggct
      421 ggtcgccttc gccggcgccg accacgtcct gctcggcagc gactacccct tcgagatgcg
      481 cgaccccacc cccgtcgcca ccgtcaacgc catccctggc ctcaccgacg agcaacgcgc
      541 cctcattctc gaaggcaacc tcctgcgcct cctcggccat ctcatcaaat gatcttcaaa
      601 tgatcttcac ttgcggcatt cacttgcggc atgggggcat tgcggtgcag cgtcgtggca
      661 cgatttgcga tagtctgcgc cgatcgtgcc gcgacccaag atgagggcgc cgggcctggg
      721 tggtcgtcgc gccgcctcgc gtcgtgctga aggaggttgt ggccatggtt gacccgggca
      781 acgcacggga caacgccgct gcggagtcgg gccggccgct gccgcagatc gacaccagcg
      841 tcccgcatcc cgcccgcgcc tacgactacc tgctcggcgg caaggacaac ttccccgccg
      901 accgggcgtc ggccgagcgc gtgctggcag ccgccccgga gatgcgcgac accgcccgct
      961 ccaaccgggc gtttctggtg cgggcagtcc gcttcctcgc cgagcagggg atccgccagt
     1021 tcctcgacat cggcaccggg atccccacca gccccaacgt ccacgaggtc gcccagcagg
     1081 tcgccccgca ggcccgcgtc gtctacgtgg acaacgaccc gctggtgctg gtccacgccc
     1141 gcgccctgct caccagcggc ccccaggggg cgaccgacta cctggaacgc cgacgtccac
     1201 gatccggagg aggtcctgcg gggcgccgca cagaccttgg acttcgccca accggtggcg
     1261 ctgctgctga tcgcgctcct gcaccttctc ccggacgccg acgatccggc ggggatcgtc
     1321 gcacggttgc tcgcgccgct ggcgcccggc agctatctgg cgctgcagca tctcggcttg
     1381 gacgccaacg agccgaaggc cctggcggtg tccgcggccg ccggacggga cggggtgacg
     1441 ctcctcccac gacgccgcac cgagatcctg cggttcttcg acggcctgga gctggtcgag
     1501 cctggcctcg tccaggacgc gctgtggcgt cctgacggcg aggtgcctgc tgagtttcgc
     1561 cgcagctggg gcttcgcggg cgtggcccgc aagtcctgac cccctcgtgg gcctcgtgaa
     1621 tcatcccggg gtcgccacac cattcggtca ctcctcgcgg tgcgggcgat tggctggtgg
     1681 gccctccacc tttcggttga gggccaggac ggcctcggcc gaagggtgga gatgaaacgc
     1741 agacctcgtg gccgacgacg ccaccgggct ggccaccgta ctgtcctctc gaagccacgc
     1801 gccccgtggc gcctgtcagc cgaaggaggg gtcctcatgc ttcagccccc gcctgtcagc
     1861 aggctcccgc agcagcgccg agccctgccc gcgcatgagc gcgtccagcg gatcg
//
LOCUS       DASWDC010000183         1928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_545196, whole genome shotgun sequence.
ACCESSION   DASWDC010000183 DASWDC010000000
VERSION     DASWDC010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545196"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /gene="pgk"
                     /locus_tag="VF995_05850"
     CDS             <1..355
                     /gene="pgk"
                     /locus_tag="VF995_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9343120.1"
                     /translation="EHRTVPVDAIEKGWRGLDIGPETARAFAAAVAEARTVFWNGPMG
                     VFEWPPFAAGTKAVAEAVASSDAYTVVGGGDSAAALAQLGLADRVDHLSTGGGASLEL
                     LEGKTLPGVAVIPPA"
     gene            367..1149
                     /gene="tpiA"
                     /locus_tag="VF995_05855"
     CDS             367..1149
                     /gene="tpiA"
                     /locus_tag="VF995_05855"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX9343121.1"
                     /translation="MARRPVIAGNWKMHKTHLEAVQLTQKTAWALNKDDTEAVEVVVC
                     PPFTALRSVGTLIEGDKLALGLGAQDCHPEPQGAYTGEISAPMLARLGCDYVIVGHSE
                     RRQYFAESDQLVNRKARAVLANDMCPIVCVGESLEQREAGQTATVVPGQVRGSLAGLS
                     AEQVGGLVVAYEPVWAIGTGRAATAADARETIALIRRTIADLAGRAAADSVRIQYGGS
                     VKAANAEELMSQPDIDGALVGGASLDADEFALIVKGSVPRRR"
     gene            1210..1446
                     /gene="secG"
                     /locus_tag="VF995_05860"
     CDS             1210..1446
                     /gene="secG"
                     /locus_tag="VF995_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017577749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX9343122.1"
                     /translation="MVPLLVILHIIASLTLCLFILLHAGRGGGLSDMFGGGMNTSLGG
                     STVVERNLDRLTVGAASLFGLTSIFLGLLLAPKG"
     gene            1556..>1928
                     /locus_tag="VF995_05865"
     CDS             1556..>1928
                     /locus_tag="VF995_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9343123.1"
                     /translation="MLVALNPGVTSRLLHRAARAAQVKRNARAQPHVGGTLTVALPLS
                     SPLDLDPAFAEQPGERTMAASLFTGLTTLDPDGTVRPALAASWSSDASLRHWRFTLRP
                     GARFSDDTPIQAADVTFAWERL"
BASE COUNT          264 a          790 c          623 g          251 t
ORIGIN      
        1 cgagcaccgc acggtccccg tcgacgcgat cgagaagggc tggcgcggcc tcgacatcgg
       61 gcccgagacc gcgcgcgcct tcgccgccgc cgtcgccgag gcccggaccg tcttctggaa
      121 cgggcccatg ggcgtgttcg agtggccgcc cttcgcggcc ggcacgaagg ccgtcgccga
      181 ggcggtcgcc tccagcgacg cctacaccgt cgtcggcggc ggcgactccg ccgccgcgct
      241 cgcccagctc ggcctggccg accgggtcga ccacctgtcc accggcggcg gggcgagcct
      301 cgagctgctc gagggcaaga ccctgcccgg cgtcgccgtc atcccgcccg cgtgaggagc
      361 cgacccgtgg cccgacgacc cgtgatcgcc ggcaactgga agatgcacaa gacccacctc
      421 gaggccgtcc agctcaccca gaagaccgcc tgggcgctca acaaggacga caccgaggcg
      481 gtcgaggtgg tggtgtgccc gccgttcacc gccctccgca gcgtcggcac cctgatcgag
      541 ggcgacaagc tggcgctcgg gctcggcgcc caggactgcc acccggaacc ccagggtgcc
      601 tacaccggcg agatctccgc gccaatgctc gccaggctcg gctgcgacta tgtgatcgtc
      661 ggccactccg agcgccgcca gtacttcgcc gaaagcgacc agctggtcaa tcgcaaggcc
      721 cgcgcggtgc tcgccaacga catgtgcccg atcgtctgcg tcggcgagtc gctggagcag
      781 cgcgaagccg gccagaccgc caccgtggtg ccgggccagg tccgcggctc cctcgccggg
      841 ctctccgccg agcaggtcgg cggcctggtg gtcgcctacg agccggtctg ggcgatcggc
      901 accggccgcg ccgcgaccgc cgccgacgcg cgggagacca tcgccctgat ccgccggacg
      961 atcgccgacc tcgccggccg ggccgccgcc gactcggtcc gcatccagta cggcggcagc
     1021 gtcaaggccg ccaacgccga ggagctgatg tcccagcccg acatcgacgg tgccctggtc
     1081 ggcggcgcga gcctcgacgc cgacgagttc gccctgatcg tcaagggctc ggtgccccgc
     1141 cggcgctgat agactgacaa cagcgtcgca cacccaaact cgtcaacccg cacgcaagga
     1201 actcgcctga tggttcccct cctggtcatc ctgcacatca tcgcctcgct cacgctgtgc
     1261 ctgttcatcc tgttgcacgc cgggcgtggc ggcggcctgt cggacatgtt cggcggtggc
     1321 atgaacacca gcctcggcgg ctccaccgtt gtcgagcgca acctcgaccg cctcaccgtc
     1381 ggcgccgcca gcctgttcgg gctcaccagc atcttcctcg gcctgctcct cgcccccaag
     1441 ggctgagctc ccggccgtgg cccgtcggca acggtcaacg ccttccggca cccgaacccg
     1501 aacccgaacc atcgccctgg cggcggggct cgccgtcgcc ctcgccgctg gcggcgtgct
     1561 cgtggccctc aacccgggcg tcaccagccg cctcctgcac cgcgccgccc gggctgctca
     1621 ggtcaagcgg aacgcgcggg cccagccgca cgtcggcggc accctgaccg tcgcgcttcc
     1681 cctcagcagc cccctcgacc tcgaccccgc gttcgccgag cagccggggg agcggaccat
     1741 ggccgccagc ctgttcaccg gcctcaccac actcgacccg gacggcaccg tgcggcccgc
     1801 cctggcggcc tcctggtcaa gcgacgcgag cctgcgccat tggcgcttca ccctccgccc
     1861 cggcgcgcgc ttctccgacg acacccccat ccaggccgcc gacgtcacgt tcgcctggga
     1921 gcgcctgg
//
LOCUS       DASWDC010000184        11053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_545474, whole genome shotgun sequence.
ACCESSION   DASWDC010000184 DASWDC010000000
VERSION     DASWDC010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11053
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545474"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VF995_05870"
     CDS             <1..930
                     /locus_tag="VF995_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEX9343124.1"
                     /translation="GQVVPPTGRQFELRRGSARAVVTEVGATLRALEIAGQPYLDGFG
                     PDQLPDGSQGQVLIPFPNRVGGGRYQFEGVGEQQLPVEEAATGNAIHGLVRWANWQPL
                     SQRASRLVMGLMLHARKGYPFVLSLRVAYTLLPDGLRVRQTARNAGVAAAPYGAGFHP
                     YLTVGSACVDDDVLRIPAASWLPVDERSLPTGTAPVDGTRFDFREPRPVGAAVLDTGY
                     TDLLRDRDGFARVSLAVPGGRPRLTVFLDGAFGFLQVYTGDTLGDPAARRRGLAVEPC
                     TCATDAFNNGMGLRVLRPGETTTGVWGVSVASS"
     gene            complement(911..1906)
                     /locus_tag="VF995_05875"
     CDS             complement(911..1906)
                     /locus_tag="VF995_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9343125.1"
                     /translation="MHELPLVEEVRPGLWSVPVPIPNSPLRFVLTYVFEVPGGVVLVD
                     PGWNAPEAIAALEAGLAGIGASVGDARGVLVTHIHPDHYGLAGWVREASGAWIGLHPA
                     DAALIPDRYEDVDDLLARTAAWLAEAGAPASELESLRDASLFLRAAVHAARPDVLLED
                     GDRPEVPGWELVAVHTPGHTPGHLCFHEERSGLLLTGDHVLPRISPNVSLHPQSGDDP
                     LGDYLASLERLRAYGDGPALAGHEGPVASLAKRIDELRAHHRDRLDETSALVAGGART
                     VWEVAQGLRWSRPWEEIRGFMRRAALGETQAHLVLLERRGMVERVAGSPISWKPR"
     gene            2011..3444
                     /gene="gndA"
                     /locus_tag="VF995_05880"
     CDS             2011..3444
                     /gene="gndA"
                     /locus_tag="VF995_05880"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014444926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX9343126.1"
                     /translation="MGSEFGLTGLAVMGQNLARNLARHGVSVAVHNRTEARTREFIER
                     YGDEGSLRATGSVEQFVTAIDRPRRIIIMVKAGPPVDAVIEELLPHLDPGDVLIDGGN
                     SFFEDTRRRERVLGERDLRFIGAGISGGEEGALHGPSIMPGGSKEAYAAVEGVLTAIA
                     ATVDGTPCCTHVGPDGAGHYVKMVHNGIEYADMQLIAEAYDLLRQALGLGAGELAEVF
                     RTWNQGDLDSFLIEITGKVLAHNDAATGRPLVDLILDEAEQKGTGRWTAQSALELGVP
                     ITAITEAVYARALSAKKDDRVAAAALLPGPDGTTAPSFSADLIHDVRDALYASKVVAY
                     AQGFEQMLVASEQYGWGIDLGAMATIWRGGCIIRARFLDRIRAAYKQDPGLRNLLLAD
                     YFRDAVAGTQGAWRRVVATAATLGVPTPAFSSSLAYYDGFRRERSPANLIQGLRDYFG
                     AHTYHRTDRPGTFHTRWSEDLQEVRVG"
     gene            3692..3886
                     /locus_tag="VF995_05885"
     CDS             3692..3886
                     /locus_tag="VF995_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9343127.1"
                     /translation="MTATEVAAVLHVTPKTVSRWAKEGKLPYMRTLGGHRRYPADVIE
                     SLAKELQIPRGGEESPAEAE"
     gene            4019..4897
                     /gene="fdhD"
                     /locus_tag="VF995_05890"
     CDS             4019..4897
                     /gene="fdhD"
                     /locus_tag="VF995_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX9343128.1"
                     /translation="MAMRPGSKTRARVRVVSDTDDRIRGDDLATEEPLELRLAAGGET
                     RTLAVTMRTPGNDFELAAGFLHGEGIVTDPARLRQLRYCTDPDLDAAQQYNIVTAELT
                     PGTMLPDLPSLERHFYTTSACGVCGKASLDALALRGCRVLPAGPRVDAEVLYALPDRL
                     RAGQGLFESTGGLHAAGLFTPDGTLQCLREDVGRHNAVDKLIGWALLQDRLPLSDGIM
                     LVSGRSSFEILQKSLAAGVPVVCSVSAPSSLAVAVAEQFGMTLVGFLRGRRFNLYAGS
                     GRVRVPRTAPAATDRG"
     gene            complement(4923..5765)
                     /gene="otsB"
                     /locus_tag="VF995_05895"
     CDS             complement(4923..5765)
                     /gene="otsB"
                     /locus_tag="VF995_05895"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX9343129.1"
                     /translation="MRRYRSPGRRHGGPGGWQITADRAGQRIEPERTALCLDFDGTLA
                     PIVTDPEAARALPGTAALLGRLAGRFAAVALVSGRPASFLATHAAAPGVRYFGHYGLE
                     EIHDGEVTVDPRLAATAGAVRAATVDLQDSPAVRGSGAYLEDKGYTVAVHLRQARDPV
                     RWAGPVEAAAREIAARHGLVILPGRLVWELRPPVQSDKGDAVRRVVAAAAAQAAVMVG
                     DDRGDLPAFAVVSELAAAGGGGLRVAVRSPEAPAELLAAADLVVDGPEGVRAFLEDLL
                     GAVA"
     gene            complement(5808..6392)
                     /locus_tag="VF995_05900"
     CDS             complement(5808..6392)
                     /locus_tag="VF995_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEX9343130.1"
                     /translation="MDADDVRDLFRAAVAEFGERVDAIRDGQWRAATPCSEWDVRALV
                     NHLVNELRWVPPLLAGTTIAEVGDRFDGDLLGDDPKAAWDDASQEALDAVAAEGAMER
                     TVHLSFGDVPGGEYVRQLFADLLVHGWDLARAIGADERLHQGLVDACATWFAQVEDGY
                     RAAGVIGPRPEVPADATPQAHLLAAFGRDPAQPV"
     gene            6736..8073
                     /locus_tag="VF995_05905"
     CDS             6736..8073
                     /locus_tag="VF995_05905"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9343131.1"
                     /translation="MTPTSTRPLDVRRVTDAEVPAWVEACNTGFYRHGVDGEVEARRQ
                     GLDLDRTWGAFDGDRIVGTLRSFATELTVPGAGGFGGHGPAAGRIMGGSPVAAGPPDG
                     SSWSVPAAALTNVTVTATHRRMGLLTRMLTADLAAAAARGEAVGILIASEYPIYGRYG
                     YGPAAEEVTYTVDARHARLAPPAAWGDTIQVELADPKTAREEAAGVYERYRAQQPGAI
                     GRDGLWWDRSAGLAPRPSGVSPQSVFWALARDQAGAVAGYVTYHVESVWEERLQSRSV
                     LEVHDLIAADPRTTARLWRYCLEADLITGVRACRGPDDLLPWLLADARDVRQSARTDH
                     LWVRVLDTPGALAARRYLTSGRVVLRVTDPLGHADGVFALEGGPGGASCVPTREPAEL
                     TLPVDVLGSAYLGGVSLALPAAAGKVEEQREGAVAAADAMLRSPVAPWCATWF"
     gene            complement(8091..8915)
                     /locus_tag="VF995_05910"
     CDS             complement(8091..8915)
                     /locus_tag="VF995_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HEX9343132.1"
                     /translation="MREAAPPRRLEPRPPPRHLAPRPPRARRPSGLLFELPVVTIVVV
                     VAMGLLVVALNKHTQSSVAAPFVPNAVENPSPSAPGAAPPSATTLAARPSTSGSGGAS
                     SPNLVINAGFESGLDGWQPGGGAHLEVTGAARSGHAALAITAMAGSTADPGVITIDLG
                     TADAQRSYDGTVWVRATTPGTVAEVNLVEYVGGQRLALDGVGVVLTDTNWRQVEVEHF
                     THMAGSLLAVEVTAPGLRQPAGLFVDDVQVQPRGFGFMWRSGQPVPPTTDTTGMTH"
     gene            9105..9629
                     /locus_tag="VF995_05915"
     CDS             9105..9629
                     /locus_tag="VF995_05915"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343133.1"
                     /translation="MRKLIGGAALFAALALALAACGGSNKTPSGSGGNAPNQPAQASG
                     VGALKAGTTPLGTLLTDGDGRTLYVLTKDKNGTSTCYGSCASNWPAFTATASAGSGVT
                     SSLLGTTKRTDGKTQVTYKNQPLYYFSGDANAGDTNGEGIGGVWFAVGPTGATIPKSG
                     AGAGAGAAPTTTKY"
     gene            9738..10559
                     /locus_tag="VF995_05920"
     CDS             9738..10559
                     /locus_tag="VF995_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX9343134.1"
                     /translation="MPEPLEDQVLKVDQPKPHPAVTGAAEHDVRTELSPRWVRAYLAG
                     VAVADSKRVRLLFESGHIPVYYVPADDVRMDLLEATSKHTRCPWKGEASYWTVRVGDR
                     VSENAAWSYLDPLPGREDIKGHIAFYWDRMDAWYEEDDEVYVHPRDPYKRVDVLNSSR
                     HVEVLVNGEKVAETRRPRLLFETGLPTRYYIPRQDVRLDLLEPTATTTQCPYKGRASY
                     FSVHAGGKVANDIAWSYPFPIPECPKIENLVAFFNEKVDIRVDGEPQPRPETHWS"
     gene            10673..>11053
                     /locus_tag="VF995_05925"
     CDS             10673..>11053
                     /locus_tag="VF995_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016470059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEX9343135.1"
                     /translation="MAPTRPGPPAADRAPTPVKIVIAGGFGVGKTTFVGAVSEIIPLT
                     TEAALTTASASLDDTSLLEHKTTTTVAMDFGRITLDQDLILYLFGTPGQDRFWFMWDD
                     LVRGAIGAVVLVDTRRLADCFAAVD"
BASE COUNT         1594 a         3994 c         3962 g         1503 t
ORIGIN      
        1 ggacaggtgg tgccgccgac gggacggcag ttcgagctgc gacgcggcag cgcccgtgcg
       61 gtggtcaccg aggtcggcgc gaccctgcgg gcgctggaga tcgccgggca gccctacctc
      121 gacggcttcg ggcccgatca gctgcccgac ggcagccagg ggcaggtcct gatcccgttc
      181 ccgaaccgcg tcggcggcgg ccgctaccag ttcgagggcg tgggcgagca gcagctgccg
      241 gtcgaggagg ccgccaccgg caacgccatc cacgggctgg tccgctgggc caactggcag
      301 ccgctcagcc agagggccag ccgcctggtg atggggctga tgctgcacgc ccgcaagggc
      361 tacccgttcg tgctctcact gcgggtcgcc tatacgttgc tgccggacgg gctgcgggtg
      421 cgccagacgg cccggaacgc cggcgtggcg gcggccccct acggcgccgg gttccacccc
      481 tacctgaccg tcgggagcgc gtgcgtggac gacgacgtct tacggatacc cgcggccagc
      541 tggctgccgg tcgacgagcg ttcgctcccg accgggacgg cgccggtgga cggcacccgg
      601 ttcgacttcc gcgagccgcg cccggttggc gcggcggtcc tggacaccgg ctacaccgac
      661 ctgctccgcg accgcgacgg cttcgcccgg gtctcgctgg cggtccctgg cgggcgcccc
      721 cgcctcaccg tgtttctgga cggcgccttc ggcttcctgc aggtctacac cggcgacacg
      781 ctcggcgatc cggccgcgcg gcgccgcggc ctcgcggtcg agccctgcac ctgcgccacc
      841 gacgcgttca acaatggcat gggcctgcgg gtcctgcgac cgggcgagac caccactggc
      901 gtctggggcg tcagcgtggc ttccagctga tcggagatcc ggccacacgc tccaccatgc
      961 cccgccgttc cagcagcacc aggtgcgcct gggtctcgcc gagcgcggcg cggcgcatga
     1021 agccccggat ctcctcccag ggccgcgacc agcgaagccc ctgggcgacc tcccagaccg
     1081 tccgagcgcc gccagcgacc agcgccgagg tctcgtcgag gcgatcccgg tggtgggcgc
     1141 gcagctcgtc gatccgcttg gcgagcgagg cgaccggccc ctcatgcccc gccagggccg
     1201 gcccgtcgcc gtaggcccgc agccgctcca gcgacgccag gtagtcgccg agcgggtcgt
     1261 cgccggactg cggatgcagc gagacgttcg ggctgatccg cggcagcacg tggtcgccgg
     1321 tcaacagcag cccgctgcgc tcctcgtgga agcagagatg gccgggcgta tggcccggcg
     1381 tgtggaccgc caccagctcc cagccgggca cctcgggccg gtcgccgtcc tccagcagca
     1441 cgtccggcct ggccgcgtgg acggcggcgc gtaggaacag gctggcgtcg cgcaggctct
     1501 ccagctcgct ggccggcgcc ccggcctcgg ccagccaggc agccgtgcgc gccaggaggt
     1561 cgtcgacgtc ctcgtagcgg tcggggatca gcgccgcgtc cgccggatgc agcccgatcc
     1621 aggcccccga cgcctcccgc acccaccccg ccagcccgta gtgatccggg tggatgtggg
     1681 tgacgagcac cccacgcgcg tccccgaccg aggcgccgat gccggcgagc cccgcctcca
     1741 gcgccgcgat cgcctccggc gcgttccatc ccggatcgac cagcaccacc ccgccgggaa
     1801 cctcgaacac gtaggtcagg acaaatcgca atggactatt cggaatcggc acgggcacgc
     1861 tccacaggcc aggccgaacc tcctcgacga gtggcagctc gtgcacgctc cgtcctcctg
     1921 ggcggttcct tgtccacaga tccaagccag catgcccggg gagcgagcac ctgtgccatg
     1981 ctcggaacga tcgatcgagc gggagggatc atgggaagcg agttcggcct gaccgggctg
     2041 gcggtgatgg ggcagaacct ggcgcgcaat ctggcgcggc atggggtgtc ggtggcggtg
     2101 cacaaccgga ccgaggcgcg gacgcgggag ttcatcgagc ggtacggcga tgagggctcg
     2161 ttgcgggcga ccggctcggt ggagcagttc gtgaccgcga tcgaccgtcc ccgccggatc
     2221 atcatcatgg tcaaggccgg gccgccggtc gacgcggtga tcgaggagtt gctgccgcat
     2281 ctcgaccccg gcgacgtgct gatcgacggg ggcaactcct tcttcgagga cacgcgcagg
     2341 cgcgagcgcg tgctcggcga acgggacctg cgcttcatcg gcgccggcat ctccggcggt
     2401 gaggagggcg cgctgcacgg gcccagcatc atgccgggag gctccaagga ggcctacgcc
     2461 gcggtcgagg gcgtgctcac cgcgatcgcc gccacggtcg acggcacccc ctgctgcacc
     2521 cacgtcggcc ccgacggcgc gggccactac gtcaagatgg tccacaacgg catcgagtac
     2581 gccgacatgc agctcatcgc cgaggcctac gacctgctcc gccaggccct cggtctcggc
     2641 gccggcgaac tggccgaggt gttccggacc tggaaccagg gcgacctcga ctcgttcctg
     2701 atcgagatca ccggcaaggt cctcgcccac aacgacgcgg ccaccggccg cccgctggtc
     2761 gacctgatcc tcgacgaggc cgagcagaag ggaaccggcc gttggaccgc acagtccgcc
     2821 ctcgagctgg gcgtcccgat caccgccatc accgaggcgg tctatgcccg cgccctgtca
     2881 gcgaagaagg acgaccgcgt ggccgcggcc gcgctgctcc ccggccccga cggcaccacc
     2941 gcgccgagct tctccgccga cctgatccac gacgtacgcg acgcgctcta cgcctccaag
     3001 gtggtcgcct acgcgcaggg cttcgagcag atgctggtcg cctccgagca gtacggctgg
     3061 ggcatcgacc tgggcgcgat ggccacgatc tggcggggcg gctgcatcat ccgcgcccgc
     3121 ttcctcgacc ggatccgcgc cgcctacaag caggacccgg gcctgcgcaa cctgctgctc
     3181 gccgactact tccgcgacgc ggtcgcgggc acgcaggggg cctggcgccg ggtggtggcg
     3241 accgcggcga ccctcggcgt gcccaccccg gcgttcagtt cctcgctcgc ctactacgac
     3301 gggttccgcc gggagcgctc gccggctaat ctcatccaag gcttgcgtga ctattttggc
     3361 gcgcatacct atcaccgcac cgatcgcccc ggcacgttcc atacgcgctg gtcagaggac
     3421 ttacaagagg tccgggtagg gtagcggcga ggaggtgtgg gcgatcctgt ttgggatctt
     3481 gcgctcgtga tcggcgccga gcgtggtcaa tggcgggtat cgtaggtttg tccatggcga
     3541 acccaaacgg gatcgtcagc catctcggta agcctacgca gggcccagta ggccgaacca
     3601 gcgatggcgc gagaatgctt gcggcaggta tgaccggcga gctagagatg cgagggggtg
     3661 gagcatgggg cagacaagcc gatcgggata catgacggcc accgaagtgg ccgccgtcct
     3721 ccatgtcacg ccgaagaccg tgtcccgatg ggccaaagag ggcaagctgc catacatgcg
     3781 aacccttggc gggcatcggc gctacccggc ggacgtgatc gaatcactcg ccaaagagct
     3841 gcagataccg cgaggtgggg aggagtcacc ggccgaggcc gagtgagcct caacagcagc
     3901 ctggccaggt ggcggtggcg accctcgtcc tgaggttcgc caccgttgcc ccggccagat
     3961 tccggtcagg ctttggcgcg cctacaatgc ccgcacaggt ctggcgggag ggttgaccat
     4021 ggcgatgcgt cccggcagca agacccgcgc ccgggtgcgc gtggtgagtg acaccgacga
     4081 tcgaatccgc ggggatgacc tcgccaccga agagcccttg gagctgcgcc tggccgccgg
     4141 tggggagacc cgcaccctgg cggtgacgat gcgcaccccc ggcaacgact tcgagctggc
     4201 cgccgggttc ctgcacggcg agggcatcgt caccgacccc gcgcggctcc ggcagctgcg
     4261 ctactgcacc gaccccgacc tggacgccgc ccagcaatac aacatcgtca ccgccgagct
     4321 gacccccggc acgatgctcc ccgacctgcc cagcctggag cggcacttct acaccaccag
     4381 cgcctgcggg gtctgcggca aggccagcct ggacgcgctc gccctgcgcg gctgccgggt
     4441 cctgcccgcg ggtcctcgcg tcgacgccga ggtcctctac gccctgcccg accggctgcg
     4501 ggccgggcag ggcctgttcg agtcgaccgg cggcctgcac gccgccgggt tgttcacccc
     4561 ggacgggacg ctgcagtgcc tgcgcgagga cgtcggccgc cacaacgcgg tcgacaagct
     4621 gatcggctgg gcgttgctcc aggaccgcct gccgctcagc gacgggatca tgctggtcag
     4681 cggccgctcc agcttcgaga tcctgcagaa gtcgctggcc gcgggcgtgc cggtggtctg
     4741 ctcggtctcg gctccctcaa gcctcgccgt cgccgtggct gagcagttcg gcatgaccct
     4801 ggtcggcttc ctccgcggcc gccgcttcaa cctctacgcc ggttccggcc gcgtccgggt
     4861 gcccaggact gcccccgccg ccaccgaccg cggctgacgc gcggccaccg acgcgcggcc
     4921 acctacgcca cagcgcccag caggtcttcg aggaaggccc gcacgccctc gggtccgtcg
     4981 accaccaggt cggccgcggc gagcagctcg gcgggggcct ccggggagcg gaccgcgacc
     5041 cgcaggccac caccgccggc cgctgccagt tccgagacca ccgcgaaggc ggggaggtcg
     5101 ccgcggtcgt cgccgaccat gaccgccgcc tgcgccgcgg cggcggcgac cacgcggcgc
     5161 accgcgtcgc ccttgtcgct ctgtaccggc ggtcgcagct cccacaccag ccggcctggg
     5221 aggatcacca ggccgtggcg cgcggcgatc tcgcgggccg ccgcctcgac gggcccggcc
     5281 caccgcaccg ggtcgcgagc ctgccgcagg tggaccgcga ccgtgtagcc cttgtcctcg
     5341 aggtaggcgc ccgacccgcg gaccgcgggc gagtcctgga ggtccaccgt ggcggcacgg
     5401 accgccccgg cggtcgccgc aaggcgcggg tcgacggtga cttcgccgtc gtggatctct
     5461 tcgagcccgt agtggccgaa atagcgaacc cccggcgcgg cggcgtgcgt ggcgagaaac
     5521 gacgctgggc gccccgacac cagcgccacc gccgcgaacc gtcccgccag ccgcccgagc
     5581 agcgccgccg tgccaggcaa cgccctggcc gcctcgggat cggtcacaat cggcgcgagg
     5641 gtcccgtcaa aatcgaggca cagcgcggtc ctctccggct cgattcgctg acccgcccga
     5701 tccgccgtaa tttgccaccc tccggggcct ccgtgcctcc tccccggcga acggtagcga
     5761 cgaattcgaa atgacgcaaa aagttatcca caggcgcgaa aagctggtca gacaggctgg
     5821 gcggggtcgc ggccgaaggc ggccagcagg tgggcctgcg gggtcgcgtc ggctgggacc
     5881 tcggggcgtg gaccgatcac gccggcggcc cggtagccgt cctcgacttg ggcgaaccag
     5941 gtggcgcagg cgtcgaccag cccctggtgg aggcgttcgt cggcgccaat cgccctggcc
     6001 aggtcccagc cgtggaccag caggtcggcg aagagctgcc ggacgtactc gccgcccgga
     6061 acgtcgccga acgacaggtg gacggtccgc tccatggcac cctcggccgc gaccgcatcg
     6121 agggcttcct gggaggcgtc gtcccaggcg gccttcgggt cgtcgccgag caggtcgccg
     6181 tcgaagcggt cgccgacctc ggcgatggtg gtgccggcca gcagcggcgg cacccagcgc
     6241 agctcgttga ccaggtggtt gacgagggcg cggacgtccc actcgctaca gggcgtggcc
     6301 gcccgccact ggccgtcgcg gatggcgtcg acccgctcgc cgaactcggc aacggcagcg
     6361 cggaacaggt cgcgaacgtc gtcagcgtcc atcgtgggtc cccctcagct ctggtcggca
     6421 cgcccgaccg aacggtcact catcgcatgg tggcacagcc cactgccagc gagcgtcgcc
     6481 gatcgccgag gcctgcccgg cgacggcagg ccattcgggc agagggcgcg ggctgtacca
     6541 tgagctgacg atggcgttct cctcgtcggg ccaggtcacc acgccggagg ctccggaggc
     6601 gttcgcggac gcggtggcga gcttgctgac ctgaccgtgc cgcaccgcgg ccagccgcag
     6661 cgctcgcgcg ccaggtcgta ggtcgtcgtg agtattcgaa tgacggccga ggtatcgcca
     6721 gctagcctgg tcgtcatgac gccgacatcg acgcggcccc tggacgtgcg gcgggtcacc
     6781 gacgcggaag tccccgcctg ggtcgaggcg tgcaataccg ggttctaccg tcacggagtg
     6841 gacggagagg tcgaggcacg caggcagggc ctggatctcg accggacctg gggggcgttc
     6901 gacggggacc ggatcgtcgg cacgctgcgg agcttcgcca ccgagctgac cgtgcccggc
     6961 gctggggggt tcggggggca cggtcccgca gccggaagga tcatgggcgg ttctcctgtg
     7021 gctgcggggc cccccgatgg atcaagttgg tccgtgccgg cggcggcgct caccaacgtc
     7081 accgtgaccg ccacgcaccg ccgcatgggg ctgctcaccc ggatgctgac cgccgatctg
     7141 gcggcggcgg ccgcgcgggg cgaggcggtc gggatcctga tcgcctccga gtacccgatc
     7201 tacggccgct acggctacgg gccggccgcc gaggaggtca cctacacggt tgacgcccgc
     7261 cacgcgcggc tggcgccacc agctgcctgg ggtgacacga tccaggtcga gctggccgac
     7321 ccgaagaccg cgcgggagga ggccgccggg gtctacgagc gctaccgcgc ccagcagcct
     7381 ggggcgatcg ggcgggatgg gctgtggtgg gaccgctccg cggggctggc gccgaggccg
     7441 agcggcgtga gcccccagtc ggtgttctgg gcgctggccc gcgaccaggc cggcgccgtc
     7501 gccggctacg tgacctatca cgtcgagagc gtgtgggagg agcggctaca gtcccgcagc
     7561 gtcctcgaag tccacgacct gatcgccgcc gacccgcgga ccaccgcgcg actgtggcgc
     7621 tactgcctcg aggccgacct gatcaccggc gtgcgcgcct gtcgcggccc cgacgacctg
     7681 ctgccctggc tgctcgccga cgcccgcgac gtgcgccaga gcgcccgcac cgaccacctc
     7741 tgggtgcggg tgctggacac ccccggggcg ctggccgctc ggcgctacct gaccagcggg
     7801 cgggtcgtgc tgcgggtcac cgacccgctc ggccatgcgg atggggtttt cgcgctggag
     7861 ggcggacccg ggggagcgag ctgcgtcccg acccgcgagc cagcggagct gaccctgccg
     7921 gtggacgtgc tcggcagcgc ctacctcggc ggggtctcgc tggcgctgcc ggccgccgcc
     7981 gggaaggtcg aggagcagcg cgagggtgcc gtggccgccg ctgacgccat gctgcgctcg
     8041 ccggtcgccc cctggtgcgc cacctggttc tgagccgcgg cgccagggcg tcagtgggtc
     8101 atgccggtcg tgtcggtggt aggtgggacc ggctggccgg agcgccacat gaagccgaag
     8161 ccgcgcggct ggacctggac gtcgtcgacg aacagcccgg ccggctggcg caggccggga
     8221 gcggtcacct cgacagcgag gaggctgccg gccatgtggg tgaagtgctc gacctcgacc
     8281 tgccgccagt tggtgtcggt gaggaccacg ccgacgccgt ccagggcgag gcgctggccg
     8341 ccgacgtact cgaccaggtt cacctcggcg accgtccccg gggtggtggc gcggacccag
     8401 acggtgccgt cgtaggaccg ttgcgcgtcg gcggtgccca ggtcgatggt gatcaccccg
     8461 ggatcggcgg tcgagcccgc catcgcggtg atcgccagcg ctgcgtggcc gctgcgggcc
     8521 gcgccggtca cctccaggtg cgcgccgccg cctggctgcc agccgtcgag gccgctctcg
     8581 aagccagcgt tgatgacgag gttggggctg gaggccccgc cgctgccgct ggtggagggc
     8641 cgggcggcga gggtggtggc gctcggtggt gccgcgccgg gtgccgacgg cgaggggttc
     8701 tcgacggcgt tgggaacgaa cggggcggcc acgctggact gggtgtgctt gttgagcgcg
     8761 accaccagca ggcccatggc cacgaccacg acgatcgtga ccaccgggag ctcgaacagc
     8821 aggccgctgg gccgccgggc acgcggcggt cgcggcgcca gatgccgtgg cggtgggcgc
     8881 ggctccagac gccgcggcgg tgctgcttct cgcatgcccg cacggtcgca ccgcggccta
     8941 atggggcggt aacggcacca cctcgggatg cccgccggcc gcgccgggga ggccggggaa
     9001 tcggcgtcga tgccatgggg ttaaccggaa gttgaacgaa agctgtaccc cgctccgtac
     9061 ggcttgtcga gtcccagagg cagagctcaa cggaggtatc caccatgagg aagctgattg
     9121 gcggcgccgc gctgttcgcg gcgctggcac tcgctctcgc ggcgtgcggt ggcagcaaca
     9181 agacaccgtc cggttccggc ggcaacgccc cgaaccagcc ggcgcaggca tcgggggtgg
     9241 gcgcgctgaa ggccggcacc acacccctcg gaacgctgct gaccgacggc gacgggcgca
     9301 ccctgtacgt cctcaccaag gacaagaacg gcaccagcac ctgctacggc agctgcgcgt
     9361 cgaactggcc ggcgttcacc gccaccgcca gcgcgggcag cggcgtcacc agctcgctgc
     9421 tgggcaccac caagcgcacc gacggcaaga cgcaggtcac ctacaagaac cagcccctct
     9481 actacttcag cggcgacgcc aacgccggcg acaccaacgg cgagggcatc ggcggcgtct
     9541 ggttcgccgt cggcccgact ggcgcgacga tccccaagtc cggcgccgga gcgggcgcgg
     9601 gcgcggcgcc caccaccacc aagtactgac ccaaccgcaa ctccgcctcc acggcaggcg
     9661 gcgtaggctc gtgacggcct accgccgcct gccgttgccg tttcccgctg ttgtcgaggc
     9721 cgaggaggcg ccccaccatg cccgagccgc tcgaagacca ggtcctcaaa gtcgaccagc
     9781 cgaaaccgca cccggcggtc accggcgccg ccgagcatga cgtccgcacc gagctttcgc
     9841 cgcgctgggt gcgcgcctac ctcgccgggg tggcggtggc cgactccaag cgggtgcggc
     9901 tgctgttcga atccggccat atccccgtgt actacgtccc ggctgacgac gtgcgcatgg
     9961 acctgctgga ggccaccagc aagcacacca ggtgcccgtg gaagggtgag gcgagctact
    10021 ggacggtccg ggtgggggac agggtcagcg agaacgccgc ctggtcctac ctcgacccgc
    10081 tccccggccg cgaggacatc aaggggcaca tcgccttcta ctgggaccgg atggacgcct
    10141 ggtacgagga ggacgacgag gtctacgtcc atccccgcga cccctacaag cgggttgacg
    10201 tgctgaacag ctcccggcac gtcgaggtgc tggtcaacgg cgagaaggtg gccgaaacgc
    10261 ggcgcccgcg gctgctgttc gagaccggcc tgccgacccg ctactacatc ccccgccagg
    10321 acgtgcgcct ggacctgctg gagccgaccg ccaccaccac ccagtgcccc tacaagggcc
    10381 gggcgagcta cttctcggtc cacgccggcg gcaaggtggc caacgacatc gcctggagct
    10441 accccttccc gatcccggag tgccccaaga tcgagaatct ggtcgccttc ttcaacgaga
    10501 aggtggacat ccgggtcgac ggcgagccgc agccgcggcc cgagacccac tggtcctagg
    10561 gtcctcgccg ggcgcggcgc cgctcgcggc cggtaggcgc cattcgggcg ccggttcccg
    10621 gactcgccga ttttcgcgtg ccgcggggtc ggcgctatat cctgacgctc ttatggctcc
    10681 cacacgcccc ggaccacccg cagcggatcg tgcgccgacg ccggtgaaga tcgtgatcgc
    10741 ggggggcttt ggggtgggca agaccacctt tgtgggggcg gtgtcggaga tcatcccgct
    10801 gaccaccgaa gcggcgctga ccacggccag tgcgtcgctt gatgacacca gtctgctgga
    10861 acacaagacc accaccacgg tggcgatgga ctttggccgg atcaccctgg accaggatct
    10921 gatcttgtat ctgtttggga cgccggggca ggatcggttc tggtttatgt gggatgacct
    10981 ggtgcggggc gcgatcgggg cggtggtgtt ggtggatacg cgccggttgg cggattgttt
    11041 cgcggcggtg gac
//
LOCUS       DASWDC010000185         3412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1297220, whole genome shotgun sequence.
ACCESSION   DASWDC010000185 DASWDC010000000
VERSION     DASWDC010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297220"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..962
                     /locus_tag="VF995_05930"
     CDS             246..962
                     /locus_tag="VF995_05930"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9343136.1"
                     /translation="MSVPETETATRFSAGAQPLSGLLRVEEQRRTAHEFVRDTLRRAI
                     LSGALAGGTRLVQADIAAQLNVSTTPVREALRDLAADGLIRFDPHRGGVVNTIDAAEF
                     QEIYEIRKRLEPYAVRRAAERISAKDLDAAAALAAAMEAEPDPGAWTELNWRFHGMLV
                     QAARSPRLLSVVKSAQDAAALYVARSLQETPSRVEQGNAEHRALLEAMRRRDGDTVER
                     ILVEHLEGTLGALKEPSQPR"
     gene            1129..2157
                     /locus_tag="VF995_05935"
     CDS             1129..2157
                     /locus_tag="VF995_05935"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9343137.1"
                     /translation="MPTVDVHTHFIPPELLADAARGQAPDGVAQEVVDGVPWLVHRQG
                     YRYPVPPPFHDVEARLAAMDALGVDVAVVSIAPTLFLYWADPDEAADFCRAANDALAR
                     FAAASGGRIVTLATLPLQAPDAAIKELHRAVAHLGLRGAEIGPRVEEVPLDDPTMLPL
                     LDTAQDLGVPLVLHPYYVGPRSGLADFYLVNLLGNPIQTTTCAARLILSGTLDDLERL
                     ELVLLHGGGFLPYQIGRMDHGNRVRPEARACRRPPSEYLRRFTYDTITHAPRPLAFLV
                     SQVGAARVAFGTDYPFDMAGGALDQQLEGAGLDHDQRERIAWRNAARLFRLDLEADAD
                     PALAPGAP"
     gene            complement(2243..2584)
                     /locus_tag="VF995_05940"
     CDS             complement(2243..2584)
                     /locus_tag="VF995_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343138.1"
                     /translation="MRACAPVPLSGQSSRVPPSAASCSRACSLTAIGSVLASTTTRSG
                     RPPFPSSLASAVASPSWPATTSPSAAAEGSEVITTGARAATARLEAAACPPLATSAST
                     RAAARSWPTTV"
     gene            2725..3105
                     /locus_tag="VF995_05945"
     CDS             2725..3105
                     /locus_tag="VF995_05945"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX9343139.1"
                     /translation="MVAVHNVAAAEALSLARRVGLDPAEVLPVLADGAGTSRMLELRG
                     PLMVAGDYGDAAMRVELWLKDVGLIGAFAREHGAPTPLFDAAAPLYHAALAQGRGAQD
                     TASVFAVLDQEQAVKGLEQQRQKE"
     gene            3109..>3412
                     /locus_tag="VF995_05950"
     CDS             3109..>3412
                     /locus_tag="VF995_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HEX9343140.1"
                     /translation="MKLVRCLVDGTVRHGRCVGDQTGTGDHDRDRLQVALVAPVAVEA
                     PDGVAADPLAGLRQARDATEVERRPLSQVRLLAPLTRPGKVVAIGLNYADHAAEAAG"
BASE COUNT          449 a         1281 c         1223 g          459 t
ORIGIN      
        1 gaacgaagcc atgcgccgtc gccctccttg cggtcgcggg atggaccgct gctggaccac
       61 atgcaaaatt tgcgtaacga ggttgcagag cgcgttgcag accccgtccg tgatcatata
      121 tcttgcaatc tgtatccaga acaaggtgcg ttgcggcggc gtgcggaacc tccgcgcgct
      181 gttgtgcgcc cagagcgatc ttccgccagg tcgttcgagg tttgaggggg ctgaaccttc
      241 ccgtcatgag cgtgccagag acagagacag cgacgcggtt ctccgccgga gcgcagccgc
      301 tctccgggct cctccgcgtc gaggagcagc gccgcaccgc ccacgagttc gtccgcgaca
      361 cgctgcggcg ggcgatcctc agcggcgccc tcgccggcgg cacccggctg gtccaggctg
      421 acatcgccgc ccagctcaac gtcagcacca ccccggtgcg cgaggccctg cgcgacctcg
      481 ccgccgacgg gctgatccgc ttcgacccgc accgcggcgg ggtcgtcaac accatcgacg
      541 ccgccgagtt ccaggagatc tacgagatcc gcaagcgcct ggagccctac gcggtgcgcc
      601 gcgccgccga gcgcatcagc gccaaggacc tggacgccgc cgccgcgctg gccgccgcca
      661 tggaggctga gccggacccc ggtgcctgga cggagctgaa ctggcgtttt cacggcatgc
      721 tggtccaggc cgcccgctcg ccccggctgc tgtcggtggt gaaaagcgcc caggacgccg
      781 ccgcgctgta tgtcgcccgc tcgctgcagg agaccccgag ccgggtcgag cagggcaacg
      841 ccgagcaccg cgcgctgctg gaggcgatgc gccgacgcga cggcgacacg gtcgagcgga
      901 tcctggtcga gcacctggag ggcacccttg gggcgctgaa ggagccgtcc cagccgcgct
      961 gacccgctcg gaccccaggt tcgttcattg gcgcagctcg atccgtgcga tccgttcgat
     1021 ccactcggtt ccgcgcgttc gggttgacga cctcgctctg atctaacagc atgcataatg
     1081 caatttatcg ggcgtggatt ccaggtcgag cggatgggag gtccagtcgt gcccacagtc
     1141 gacgtccata cccacttcat cccgccggag ctgctggccg acgccgcccg ggggcaggct
     1201 cccgacgggg tggcgcagga ggtggtcgac ggggtcccat ggctggtcca ccggcaggga
     1261 taccgctacc cggtgccgcc gccgttccac gacgtcgagg cgcggctggc cgcgatggac
     1321 gccctcggcg tggacgtggc ggtggtgtcg atcgcgccga ccctgttcct ctactgggcc
     1381 gaccccgacg aggccgcgga cttctgccgc gcggccaacg acgcgctggc ccgcttcgcc
     1441 gccgcctccg gcgggcgcat cgtgacgctg gcgaccctgc cgctgcaggc acccgacgcc
     1501 gccatcaagg agctgcaccg cgccgtcgcc cacctcggcc tgcgcggcgc cgagatcggc
     1561 ccccgggtgg aggaggtccc gctcgacgac cccaccatgc tgccgctgct ggacaccgcc
     1621 caggacctcg gcgtcccgct ggtgctgcac ccctactacg tcgggccccg ctccgggctg
     1681 gccgacttct acctggtcaa cctgctcggc aacccgatcc agacgaccac ctgcgcggcc
     1741 cggctgatcc tcagcggcac cctcgacgac ctggagcggc tggagctggt gctgctgcac
     1801 gggggcggct tcctgcccta ccagatcggc cgcatggacc acggcaaccg ggtccgcccg
     1861 gaggcccgcg cctgccgccg ccccccgtcg gagtacctgc gccgcttcac ctacgacacc
     1921 atcacccacg cgccccggcc gctcgccttc ctggtcagcc aggtcggcgc ggcgcgggtg
     1981 gcgttcggca ccgactaccc gttcgacatg gccggcggcg cgctcgacca gcagctggag
     2041 ggcgccgggc tcgaccacga ccagcgcgag cggatcgcct ggcgcaacgc cgcgcggctg
     2101 ttccgcctgg acctcgaggc cgacgccgac ccggcgctgg cgccgggtgc gccgtgagcg
     2161 ccgccggcgc cgtgggcgtg gttggcgtgg tgggcctcgg gacgatgggg ggcgccatcg
     2221 ccgccaacct gctgcgcaac ggctacaccg tggtcggcca tgacctggcc gccgcgcggg
     2281 tcgacgcgct cgtcgccaaa ggagggcagg cggcggcctc cagccgggcg gtcgccgcgc
     2341 gcgccccggt ggtgatcacc tcgctgccct cggcggccgc gctgggcgag gtggtggcgg
     2401 gccatgaagg gctggcgacc gcgctcgcca acgagctggg gaacgggggg cggcccgacc
     2461 tggtggtggt cgaggcgagc acgctgccga tcgccgtcaa ggagcacgcc cgcgagcagc
     2521 tcgcggcgct gggcggcacc ctgctggact gcccgctgag cggcaccggc gcgcaggcgc
     2581 gcacggggga cctggtggtg ctcggcagcg gcgaccccgc cgcgctacgc cgctgcgagc
     2641 cggtgttcga cgccttcgcg cgctcctggt acgacctggg cgccttcggc aacgggtcaa
     2701 aggtgaagct cgtcggccaa cctgctggtc gcggtccaca acgtggcggc ggccgaggcg
     2761 ctgtcgctgg cccgccgcgt cggcctcgac ccagccgagg tcctgccggt gctggccgac
     2821 ggcgccggga cctcgcggat gctggagctg cgcggcccgc tgatggtggc cggcgactac
     2881 ggcgacgccg ccatgcgggt ggagctgtgg ctcaaggacg tcggcctgat cggcgcgttc
     2941 gcccgcgagc acggcgcgcc cacgccgctg ttcgacgccg ccgcgcccct ctaccacgcg
     3001 gccctggcgc agggccgcgg cgcgcaggac accgcctcgg tcttcgccgt gctggaccag
     3061 gagcaggccg tgaagggcct ggagcaacag cgacagaagg agtgagccgt gaagctcgtg
     3121 cgctgcctcg tcgacgggac cgtccgccac ggccgctgcg tcggcgacca aactggcacc
     3181 ggcgaccacg accgtgaccg gctccaggtc gctctggtcg ccccggttgc ggtggaggcg
     3241 cccgacggtg tggccgccga cccgctcgct gggctgcgcc aggcacgcga cgccaccgag
     3301 gtggagcgcc ggccgctgtc gcaggtacgg ctgctcgcgc cgctgacccg ccccggcaag
     3361 gtggtggcga tcggactcaa ctacgccgac cacgccgccg aggccgccgg ca
//
LOCUS       DASWDC010000186        12850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_545621, whole genome shotgun sequence.
ACCESSION   DASWDC010000186 DASWDC010000000
VERSION     DASWDC010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12850
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545621"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VF995_05955"
     CDS             <1..812
                     /locus_tag="VF995_05955"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX9343141.1"
                     /translation="DHLLGRQLDHAGLGRDEVIVTEGALRRVVGDYTREAGVRNLERE
                     IGKLLRKVATQIAAGEKDGKVVVDEGDVPGYLGRQRFFFEAADRTAVPGVATGLAVTG
                     FGGDVLFIEASAMNGEPGLTLTGQLGDVMKESATIALSYVRAHAAELGLDPEEIARRR
                     VHLHVPAGAVPKDGPSAGTAMTTALVSLLSGRPVRAEVGMTGEVTLQGRVLPIGGVKQ
                     KVLAAHRAGLKEVILPERNAGDLEDVPESVREQLRFHLAAEIGDVLKEALA"
     gene            complement(887..1390)
                     /locus_tag="VF995_05960"
     CDS             complement(887..1390)
                     /locus_tag="VF995_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX9343142.1"
                     /translation="MSLISLIRRPAVLAAALLSALTIAGPAAGQALASTPRDLRVSSA
                     TCGGVSVVVQGMPASQQLFLLVRNVADGKTLDGPAPVHSDSDGSVQSSLRVDLSAVRT
                     VDVSIWTKQGATLTMAAKDTAVTSCGHLPMTGDSSSRDALLAVALVAIGALVVWSTRS
                     RRTRATR"
     gene            complement(1424..2257)
                     /locus_tag="VF995_05965"
     CDS             complement(1424..2257)
                     /locus_tag="VF995_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343143.1"
                     /translation="MALFAAALSPRDELTVVESVGELAATLQDQPDADAVMLDLPTQL
                     RREVSEQVRERYRGRLLVAADQEAETRDWSADHARRFLIRPFPVTELSRALRMPIAPV
                     AAPPPAQVPEEVPEELIPMEADAGAGAEPPPRRLSSAEPVWTIPSLAQAASAPAAAET
                     APELRVSGARQLSLLLAALLVLLAGVAIGGVAIGRSTAKAAPVESIATTPSGSGPPTS
                     PANPAPGQATQTKTPPACDAALTDADAAISYLIAHITDERLSKALGQFQTDRRACRQV
                     K"
     gene            complement(2588..4183)
                     /locus_tag="VF995_05970"
     CDS             complement(2588..4183)
                     /locus_tag="VF995_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018807175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343144.1"
                     /translation="MAHPQPALLDDSHVKRRTMLKAGGVLAGAAATFGMLEALADKPL
                     RLKAGAATTPLPDIQFAIGDFIPPAFTRNDGAGDVEVRLGPVFQNMTTARLTRTPTLA
                     DQTALANALNTIEQVFTFSPSGVFTWIAYGAPYFNRLRFGTRGGATATRLIPRLASNP
                     TRSVLEEMVAAPTDFGQPGITKRAFNVPVRIERNDLVIILKSDSTANIQSVLGWLSGS
                     NRLGGQSIPSPRLPIQFTSTRVIFQQPGLTRKVADQNNLSYADRINPNSPMWMGFWDQ
                     QTNAAADAATVTFAGSSVAHLTTAHAGDYFDNGSILPLSHVILDLAQFYAKAGDAAQV
                     GEDAEDYSERVQYAFRSNPIPNPGNAADGFTNGGGPSLIPNTFQGADDALKDAQGQND
                     ETPTPRLGHEQCLQRSSRAADGTPLHIRVDGSGFSSMDVPDGSNQPTLEFAAFVPTSD
                     FFNTMRRNVAALDLQEQFNVDPTDNGFERFSTATRRQMFLTPPRRHRAFPLLELTSGG
                     TGGGGGTGGTGGGGTGGGGRRRR"
     gene            5092..5313
                     /locus_tag="VF995_05975"
     CDS             5092..5313
                     /locus_tag="VF995_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HEX9343145.1"
                     /translation="MSRTSRPTYLRTSEVAGLLHVSPKTVSRWAQEGKLPFLRTLGGH
                     RRYPDSEIRALADELRMWPVVETPRAENE"
     gene            complement(5344..5793)
                     /locus_tag="VF995_05980"
     CDS             complement(5344..5793)
                     /locus_tag="VF995_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9343146.1"
                     /translation="MQEPTARTAHPTAHLLLPCRSDSVPTVRALLSRDLDRWGIAEDA
                     ADSILLATSEAVTNAVVHGSWGERESVMVIRWALSDGVLFTFQVQDRGPGLDNSRAAI
                     SRRAHPSQNRGRGLYIMHALMDRVVVDAGLNGTRILLEKLLDAPTAG"
     gene            complement(5874..6728)
                     /locus_tag="VF995_05985"
     CDS             complement(5874..6728)
                     /locus_tag="VF995_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX9343147.1"
                     /translation="MSPRRTVTAGQQLLPGFDAGRAAVLSPSALERYRLCPRLYRFLY
                     VDGQRSRSRMTAPQSFGTTVHAALREFFRLPVARRSLDGLLELFKRSWVREGYRNAAE
                     QDRERARGVEALRAWYERADTTVVPQATELSLQAAWRGVVFKGRLDRVDADRGPVGVG
                     ARNGGGNGGGLVVVDYKTGRRPVTQAAADADEALTIYAALVERRLERPVTGLVLDFVV
                     AGEQIRTERPPEVLRERLGEILDVAATLRKDERFTPRTGPWCASCEMLGLCPEGQREI
                     GRRAAELD"
     gene            complement(6754..7614)
                     /locus_tag="VF995_05990"
     CDS             complement(6754..7614)
                     /locus_tag="VF995_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX9343148.1"
                     /translation="MPSSGFDLHTHSTRSDGTLTPDEVVALAAERGLAGIALTDHDTT
                     DGVEQARAAAARGPAVAVLTGVELSAELEGQPVHVLGLAFDPTEPNFARRREWIRSGR
                     VGRARQMVERLRELGAPVSIERVLELADGGSVGRPHVARAMVEAGVVASVPDAFAPEW
                     IGTGGRAYVPKDAVTPVEAVRLIRGAGGVAVLAHPSIHAGARPVPEPTIRAMAEAGMA
                     GLEVDHPDQPPTDRARWRALAAELGLETTGASDDHGALTGYRLGVERTPEAVVERLLA
                     RAEPTDIPAE"
     gene            8160..8417
                     /locus_tag="VF995_05995"
     CDS             8160..8417
                     /locus_tag="VF995_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEX9343149.1"
                     /translation="MATAVNQPPSPPPPPPRARRAGSSWWYLLLLVPFVGTLWPPFYA
                     KATPRLGGFPFFYWYQFLMVLVGVAVTVVVYLATTRRRAGR"
     gene            8414..9979
                     /locus_tag="VF995_06000"
     CDS             8414..9979
                     /locus_tag="VF995_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019072164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX9343150.1"
                     /translation="MNINGVALSVFIFFFLLVTVLGFVAARWRRADLRHLNEWGLGGR
                     SFGTVITWFLLGGDLYTAYTFVAVPALVFGTGAPGMFALPYTIIVYPLVFLIMPRLWS
                     VAHRHQYVTPSDFVRGRYGSPGLALAVALTGILATMPYIALQLVGIQVVLQAMGISGS
                     GVARDLPLFLAFAILAVYTYQSGLRAPALIAFVKDALIYITVLVAIFYIPHKLGGWSH
                     IFGAAQATLPHRAKPASLILTPKQYVSYSTLALGSALALFMYPHSITGVLSARDRNVV
                     RRNAAILPAYSFLLGLIALLGYMAIAAGVKTSNANQAVPLLFNLTFPSWFRGFAFAAV
                     AIGALVPAAIMSIAAANLFTRNVYREFFRPDATPAEEAGVARLVSLVVKLGALAFILL
                     VKQDFAIQFQLLGGVWVLQTFPAIVLGLYTRWFHRWALLCGWAVGMASGTWMAATQHF
                     KQSTVPFNLFGIKFTAYAALAAFVLNLLVAMVLTPLFNAMRAPSGSDETTPPDYEVEA
                     APPIRVGTGEPSL"
     gene            10011..10466
                     /locus_tag="VF995_06005"
     CDS             10011..10466
                     /locus_tag="VF995_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9343151.1"
                     /translation="MSDERDPPAGRPPVPTVAVGLVARDPSGRLLLVRRGHPPSEGLW
                     SLPGGRVEAGETLAEAAARELREETGLAARIGTIAGVVERIGEGYHYVIADLWAELDG
                     DSPAPAAASDAAAVRLVELEALPGLDLTPGLAEFLEGIGCWPPGEHAPG"
     gene            10491..11303
                     /gene="mutM"
                     /locus_tag="VF995_06010"
     CDS             10491..11303
                     /gene="mutM"
                     /locus_tag="VF995_06010"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX9343152.1"
                     /translation="MPELPEVESVRRQLEPELRGRRVVAVWADRQRRFHQVERLAGHT
                     VEAVGRRGKFLVCPLDGGLELVMHLGMTGVLGIRRAGLDGEAAPYTRARLSLDDGREL
                     TFQDVRRFGGLAVVEAGAYGTVLPALARLGPEPLSDAFDPARFARDLAATRAPVKPYL
                     LSQRPVAGVGNIYADEALWLARIHPASRRVGRARALLLHAAIREVLAQAIEREGTTFR
                     DYRMLNGESGRNATFLTAYGQAGRPCPRCGTPLRKITLGGRGTTFCPTCQRH"
     gene            11326..11967
                     /locus_tag="VF995_06015"
     CDS             11326..11967
                     /locus_tag="VF995_06015"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEX9343153.1"
                     /translation="MPEGSEPELSPRASDAERNQTVDQLRTAFVEGRLNDDEFDQRMR
                     AALTARTHADLNRLLTDLPQATVVAAPATATPSPLIGGTAPGAPATHWAVAVMSGTRR
                     HGRWKAPPAINAVAIMGGCELDFRAATFSSQTTTVTVACIMGGVEITVPPGIRVEWHG
                     FALMGGMEDRVDETDLPAGAPVLIVRGFVMMGGVEIQTKAPKDKDNGQRRALP"
     gene            complement(11979..12782)
                     /locus_tag="VF995_06020"
     CDS             complement(11979..12782)
                     /locus_tag="VF995_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011321013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9343154.1"
                     /translation="MRDTWDPAQYERFAAERNAPFFELLALVEPAPGGRAADLGCGTG
                     ALTAELHRHLGASETVGIDNSAAMLARTEAAAGGGLHFESGDIAAFGARARAESGAGG
                     GWDVLFSNAALQWVSDHEALLTGFAAALAPGGQLAVQIPANDDHLAHVVAWELAGEAP
                     FREVLGGGGRPVNVREPDWYALALDRLGFVEQHVRLQVFLHHLPGPADVVEWVKGTLL
                     TWYQRHLPAELYERFLAAYRERLLERLGDLHPYPYPYKRILFWGRLPGR"
BASE COUNT         1767 a         4553 c         4616 g         1914 t
ORIGIN      
        1 gcgaccacct gctcggccgc cagctcgacc atgccggtct cggccgcgac gaggtgatcg
       61 tgaccgaggg cgccctccgg cgcgtggtcg gcgactacac ccgcgaggcc ggcgtccgca
      121 acctggagcg cgagatcggc aagctgctgc gtaaggtggc gacccagatc gcggcgggcg
      181 agaaggacgg caaggtggtc gtcgacgagg gcgacgtgcc cggctacctc gggcgccaga
      241 ggttcttctt cgaggcggcc gaccgcaccg cggtgcctgg cgtggccacc ggcctggcgg
      301 tcaccggatt cggcggcgac gtgctgttca tcgaggcgag cgccatgaac ggcgagcccg
      361 ggctgaccct gaccgggcag ctcggcgacg tgatgaagga gtcggccacg atcgcgctct
      421 cctatgtccg cgcccacgcg gccgagctgg gcctggaccc ggaggagatc gccaggcggc
      481 gcgtccacct gcacgtcccg gcgggggcgg tgcccaagga cggccccagc gccgggaccg
      541 ccatgaccac cgcgctggtg agcctgctca gcggccggcc ggtccgtgcc gaggtgggca
      601 tgactggcga ggtcaccctc cagggccgcg tcctgccgat cggcggcgtc aagcagaagg
      661 tgctggccgc ccaccgcgcc ggcctcaagg aggtcatcct gcccgagcgc aacgccggcg
      721 acctcgagga cgtgccggag tcggtgcgcg agcagctgcg cttccacctc gccgccgaga
      781 tcggcgacgt cctcaaggag gcgctcgcgt aaggcgctcg caagcctcgg ggggaggcaa
      841 cgaaagggcc cgggcggatc tgcccgggcc ctttggcgtg ccaggcctat cgggtggccc
      901 tggtcctgcg gctgcgggtg ctccagacca ccagcgcgcc gatggccacg agcgcgaccg
      961 cgagcagcgc gtccctgctg ctggagtcac ccgtcatcgg caggtgcccg cagctggtca
     1021 cggcggtgtc cttggcggcc atcgtcagag tcgcgccctg cttggtccag atgctcacgt
     1081 ccaccgtgcg gacggcgctc aggtcgaccc gcagggacga ctgcaccgag ccgtccgagt
     1141 ccgagtgaac cggcgccggc ccgtccaggg tcttgccgtc ggcgacgttg cggaccagca
     1201 ggaacagctg ctggctggcg ggcatcccct ggacgacgac gctcacgccg ccgcaggtcg
     1261 cgctgctcac ccgcaggtcc ctgggcgtcg acgcgagcgc ctggccggcg gcggggccgg
     1321 cgatggtcag tgcgctcagc aaggcggcgg ccaggacggc gggacggcgg atcagtgaga
     1381 tcagtgacat gtggttcccc cccattgagt agggtcggtg gtcctatttg acttggcggc
     1441 aggcccggcg gtcggtctgg aactggccca gcgccttgct gagccgttcg tcggtgatgt
     1501 gcgcgatcag gtacgagatg gccgcgtccg cgtcggtgag cgcggcgtcg caggcgggtg
     1561 gcgtcttcgt ctgggtcgcc tggcccggcg cgggattcgc ggggctggtc ggcggcccgg
     1621 atccagacgg ggtcgtggcg atgctctcga ccggcgccgc cttggcggtg gagcgcccga
     1681 tcgcgacgcc gccgatcgcg acgccggcca gcagcacgag cagcgccgcc agcagcagcg
     1741 aaagctgccg cgcgccgctc acccgcagct ccggggccgt ctcagcggcg gccggggccg
     1801 aggccgcctg ggccagggac gggatggtcc agaccggctc ggcgctgctg agccgccgtg
     1861 gtggtggctc ggctcccgct ccggcatctg cttccattgg gatcagctcc tctgggacct
     1921 cctcggggac ctgggcgggt ggaggggcgg cgaccggggc gatcggcatc cgcagcgccc
     1981 tcgacagctc ggtcacgggg aaggggcgga tcaggaagcg gcgggcgtgg tcggccgacc
     2041 agtcccgcgt ctcggcctcc tgatcggccg cgaccagcag acggccgcgg tagcgctcgc
     2101 ggacctgctc actgacctcg cggcggagct gggtgggcag gtcgagcatg accgcgtcgg
     2161 catcgggttg gtcctggagg gtcgcggcca attccccgac cgattccacc acggtcaact
     2221 cgtcccgcgg cgagagggcg gcggcgaata acgccaccag gctggtgtcc ctggagagga
     2281 ggacgacctg acgcggcgct ggatggcgcc tggccggccg tgctgtcggt gaccgcattt
     2341 cctgttcgcg aggctcggtc aaggtcagcc tcccccccga cacgaaagaa tcccccggcg
     2401 attttcgcca ggggattcga acttctcgcg ccttccgctg tcaagagcca actgacgtag
     2461 agcgctgctg ttttcgtttt cctgtcgagc cagccaacca gctcaggcaa tcagatcgga
     2521 catgaatatc ggtgcggggg gagcccgtcc ctccccccac accgattcat gccgccgccg
     2581 tcgtcaacta ccgccggcgg cgtcctcctc caccggtgcc gccgccaccg gtgccaccgg
     2641 tgccaccgcc gccaccggtg ccgccgctgg tgagctcaag cagtgggaag gcgcggtgcc
     2701 ggcgcggggg cgtcaggaac atctgccggc gagtggcggt gctgaaccgc tcgaagccgt
     2761 tgtcggtcgg gtcgacgttg aactgctcct gcaggtcgag cgccgcgacg ttgcgccgca
     2821 tggtgttgaa gaagtcgctg gtcgggacga atgcggcgaa ctcgagcgtc ggctggttgg
     2881 agccgtcggg cacgtccatc gaggagaagc cggagccgtc cacgcggatg tggagcgggg
     2941 tgccgtcggc cgcccgcgag gaccgctgca ggcactgctc gtggccgagg cggggcgtcg
     3001 gcgtctcgtc gttctgcccc tgggcgtcct tgagggcgtc gtcggcgccc tggaacgtgt
     3061 tggggatcag ggacgggccg ccgccgttgg tgaagccgtc ggcggcgttg cccgggttgg
     3121 ggatcgggtt ggagcggaag gcgtactgta cccgctccga gtagtcctca gcgtcctcgc
     3181 cgacctgggc ggcatcgcct gccttggcgt agaactgcgc caggtcaagg atcacgtggg
     3241 acagcgggag gatggagccg ttgtcgaagt agtcgccggc atgggcggtg gtgaggtggg
     3301 cgacgctgct gcccgcgaag gtgacggtcg cggcgtccgc ggcggcgttg gtctgctggt
     3361 cccagaagcc catccacatc ggcgagttgg ggttgatccg gtccgcgtag gagaggttgt
     3421 tctggtcggc gaccttccgc gtcaggccgg gctgctggaa gatgacccgg gtcgaggtga
     3481 actggatcgg caggcgcggc gacgggatcg actgcccgcc gaggcggttg ctgcccgaca
     3541 gccagccgag cacgctctgg atgttggcgg tgctgtcgct cttcaggatg atgaccaggt
     3601 cgttgcgctc gatccgcacc ggcacgttga acgcccgctt ggtgatgccg ggctgcccga
     3661 agtcggtcgg cgcggccacc atctcctcca gcaccgaacg cgtcgggttg gacgccaggc
     3721 gcgggatgag ccgggtggcc gtggcaccac cgcgggtccc gaagcgaagc cggttgaagt
     3781 agggggcgcc gtaggcgatc caggtgaaca cgcccgacgg cgagaacgtg aacacctgct
     3841 cgatggtgtt cagcgcgttg gccaaggcgg tctggtcggc cagggtgggc gtgcgcgtca
     3901 gccgggcggt ggtcatgttc tggaacaccg ggccgaggcg cacctcgacg tcgccggcgc
     3961 cgtcgttccg ggtgaacgcc ggcgggatga agtcgccgat ggcgaactgg atgtcgggga
     4021 gcggagtggt cgccgctccc gccttcaggc gaagcggctt gtcggccagg gcttcgagca
     4081 tgccgaaggt tgccgccgca ccggcgagca cgccgccggc tttgagcatg gtgcgccgct
     4141 tgacatgcga gtcgtcaagc agggctggct gggggtgagc cattgccggt ctccttctgc
     4201 cttctgccgg ctggtcatca ggtgaccgcc ggcgacacgt gggggtcgtg ctggcaggac
     4261 tgggatcgtt ccggcacgcg acggggtcgt gcgggtacgt ggaactctgc tcaagcagag
     4321 gtgcgcaaca tgaagctggc tatcgccgcc tccttctttc cgcctgccgg ctgatcgtcc
     4381 ggatcgccgg cgacacgtcg gggtcgtgcg taacgcgatg gggggatcgc gtgacgggaa
     4441 gaggtgttga ttgcagcgaa ccatacagca ccgcgactaa gtttccaaat ccgcatgaaa
     4501 cgacggctgc gcaatgggag aggcaatgcg aggacaccga gaaggcgaag acccgccgct
     4561 gggcgcaacc agcccgtgca gcgcaaattc cgtgcaacag gcgtgcaaat atcgatgcaa
     4621 cggaacatgg agcgtggaag gccttgagaa ggcaaaaaga aagggcggcg ccgcgacccg
     4681 ctcgctgccg accgaccgct cagcggttca ggctgcgagg tccccccagg aagccgcgac
     4741 gccaccttca ctaggacagg cgtgatcgga gccggatgga tacgggcgag gcggaagttt
     4801 ttcgccgatc agccgcgagc tgccgcttcc cgaccgatct caccgaacgt gacgggtccc
     4861 cacatactga gatctcggca atgtccccca cttgcccgca agcccgtcgc gaaccccgtt
     4921 atcatccctc tgagctgcgg aaatcgcttg atcgctcgca acgagcgcta cgactgattc
     4981 ggggatctct aggtagaaca acggattcag tagttttgtc gaagacaggt agtgtccccg
     5041 gtgggacaac agccgtgtcc aacccggacg accaccgaag gggtgtcgcg catgagccgc
     5101 accagccgac cgacctacct acggacctcc gaggtcgctg gcctcctcca tgtctcgccg
     5161 aagaccgtgt cacgttgggc ccaggaaggc aagctcccct tcctccgcac cctcggcggg
     5221 caccggcgct acccggactc cgaaatccgc gccctcgccg acgagctgcg catgtggccg
     5281 gtggtggaga ccccccgcgc cgagaatgag taaccgccgt tcccacggcg ggctggcggc
     5341 tcgctagcct gccgtggggg cgtccagcag cttctccagc aggatgcggg tcccgttcag
     5401 gcccgcgtcc accaccaccc ggtccatcag ggcgtgcatg atgtagagcc cgcggccgcg
     5461 gttctgggaa ggatgggcgc gccgcgagat cgccgcccgg gagttgtcga gcccagggcc
     5521 ccggtcctgg acctggaagg tgaacaggac gccgtccgaa agcgcccagc ggatcaccat
     5581 caccgattcc cgctcgcccc aggacccgtg gacgacggcg ttggtgaccg cctcgctggt
     5641 ggcgagcagg atgctgtcgg cggcgtcctc ggcgatgccc cagcggtcca ggtcgcgcga
     5701 gagcagcgcc cgcacggtcg gcaccgagtc gctgcggcac ggcagcagca gatgcgctgt
     5761 cgggtgtgcc gtcctcgccg tcggttcctg cactggttct ccccgtttgt agcgctttcg
     5821 cctggttcaa agagccacgc gcggcccctc tctgatacaa cggaccgggc tgatcagtcc
     5881 aactcggcag ctcggcggcc gatttcccgc tgcccctccg ggcacagccc gagcatctcg
     5941 caggaggcgc accagggccc cgtccggggc gtgaaccgct cgtctttccg cagcgtggcg
     6001 gccacgtcga ggatctcgcc gaggcgctcg cgcagcacct cgggcgggcg ctcggtgcgg
     6061 atctgctcgc cggcgaccac aaaatccagc accagcccgg tgaccgggcg ctccagccgc
     6121 cgctcgacca gcgccgcgta gatggtgagc gcctcgtccg cgtcggctgc cgcctgggtc
     6181 accggccggc gcccggtctt gtagtcgacc accaccaggc cgcccccgtt gccgcccccg
     6241 ttccgggcgc ccacgccgac ggggccgcgg tcggcgtcga cccggtcgag gcgccccttg
     6301 aacaccaccc cgcgccaggc cgcctgcagg ctcagctcgg tggcctgagg gacgacggtg
     6361 gtgtcagcac gctcgtacca ggcgcgcagc gcctcgacgc cgcgggcccg ctcgcggtcc
     6421 tgctccgcgg cgttacgata gccctcccgc acccacgacc gcttgaagag ctccagcagc
     6481 ccgtcgaggc tgcggcgcgc caccggcagc cggaagaact cgcgcagggc cgcgtgcacg
     6541 gtggtcccga acgactgcgg cgcggtcatg cgcgagcgcg agcgctgccc gtcgacatag
     6601 agaaaccggt acaggcgtgg gcagagccgg tagcgctcca gcgccgacgg gctcaacacc
     6661 gccgcgcggc cggcgtcgaa cccgggcagg agctgctgcc ccgccgtgac cgtcctccga
     6721 gggctcatcc tctgaggcta cgccacccct gtgtcattcg gccggaatgt cggtcggctc
     6781 ggctcgcgcc agcaggcgct ccaccacagc ctccggggtg cgctcgacac ccaggcggta
     6841 gccggtgagg gcaccgtggt cgtcggaggc gccggtcgtc tccaggccga gctcggcggc
     6901 cagggcgcgc cagcgggcgc gatcggtggg cggctggtcg gggtggtcga cctccaggcc
     6961 ggccatgcca gcctcagcca tggcgcggat ggtgggctct ggcactggtc tggcaccggc
     7021 gtggatcgag gggtgggcga gcaccgccac cccgcccgcg ccgcggatga gccgcaccgc
     7081 ctcgaccggc gtgaccgcgt ccttgggaac gtaggcgcgg ccgccggtgc cgatccactc
     7141 gggcgcgaac gcgtccggga cgctggcgac caccccagcc tccaccatgg cgcgggccac
     7201 gtgcggccgc ccgaccgacc cgccgtcggc gagctccagg acccgctcga tgctgaccgg
     7261 cgcgccgagc tcccgcaggc gctcgaccat ttggcgcgcc cgtccgaccc gccccgatcg
     7321 gatccattcg cgccgtcgcg cgaagttcgg ctcggttggg tcgaaggcca ggccgagcac
     7381 gtggaccggc tgcccctcca gctcagcgga cagctccacc ccggtgagca cggccacggc
     7441 gggtccgcgg gcggcggccg cgcgggcctg ctcgaccccg tcggtggtgt cgtgatcggt
     7501 gagggcgatg ccggccaggc cgcgctcggc ggccagcgcc accacctcgt cgggggtcag
     7561 ggtcccgtcg gagcgggtgg agtgggtgtg gaggtcgaat ccggaggagg gcatgggccg
     7621 agggtaccgg cgtggcaccg gccggcgccc gaacgcctcc ggagcggcct ccggagcggc
     7681 catcgggcgg ccagcggagc ggacgaccgg aagaggtgcc cgaacgacga agaacccgcg
     7741 ctgctcggcc gcgccgcggc tccgccttgg acacaatccg gtaacggcgt ggttggctcg
     7801 gccattcgac gccgatactc cgtgcaaaga ttgccgtatt gcttcccgcg gcgaggaggt
     7861 cctcgacaac tgtgtctttg ctgatgctcg ccatcgtcgc gctcttcccg gtcagcttgc
     7921 tcgccattct gctgttgact tccgggctgg agggacgcgt gatcagctcg gagtcggcgc
     7981 agacccccgt tggcggaaag cacctgcgca gccgcctccg ctccaggacc aggaccggaa
     8041 ccggaagctg agcgccctct cccccagagc ctccatctag gtttccaccg cggctggcgg
     8101 tggtattcgc ttccgcgtca ccactcgtct ggcgtggacg agacaacctg gaggtcatca
     8161 tggccacggc ggtcaaccag cccccgtccc cgcctccccc gccgccgcgg gcccgccgtg
     8221 ccgggtcgtc gtggtggtac ctgctgctgc tggtgccgtt cgtcggcacc ctgtggccgc
     8281 cgttctacgc caaggccacc ccgcgcctcg gcggcttccc gttcttctac tggtaccagt
     8341 tcctcatggt gctggtgggg gtggcggtca ccgtcgtcgt ctacctggcg accacgagga
     8401 ggagggctgg ccggtgaaca tcaacggggt cgccctctcg gtcttcatct tcttcttcct
     8461 gctggtcacc gtgctcgggt tcgtcgcggc ccgctggcgc cgcgcggacc tgcgccacct
     8521 caacgagtgg gggcttggcg gccgcagctt cggcaccgtg atcacctggt tcctgctcgg
     8581 cggcgacctg tacaccgcct acaccttcgt ggccgtgccg gcgctggtgt tcggcaccgg
     8641 cgcgccgggc atgttcgcgc tgccctacac gatcatcgtc tatccgctgg tgttcctgat
     8701 catgccgagg ctgtggtcgg tcgcccaccg ccatcagtac gtcactccct cggacttcgt
     8761 gcgcggccgc tacggcagcc cgggcctggc gctggcggtg gcgctcaccg ggatcctggc
     8821 gaccatgccc tacatcgcgc tgcagctggt gggcatccag gtggtgctgc aggcgatggg
     8881 catcagcggc agcggcgtgg cccgcgacct gccgctgttc ctcgccttcg cgatcctggc
     8941 ggtctacacc taccagagcg ggctgcgcgc cccggcgctg atcgccttcg tcaaggacgc
     9001 gctgatctac atcaccgtcc tggtggccat cttctacatc ccccacaagc tcggcggctg
     9061 gagccacatc ttcggcgctg cccaggcgac gctgccgcac cgggccaagc cggcctcgct
     9121 gatcctgaca cccaagcagt atgtgtcgta ctcgacgctg gcgctcggct cagcgctggc
     9181 cctgttcatg tatccgcatt cgatcaccgg ggtgctgagc gcgcgcgacc gcaacgtggt
     9241 gcggcgcaac gccgcgatcc tgcccgccta cagcttcctg ctcgggctga tcgccctgct
     9301 cggctacatg gcgatcgccg ccggggtcaa gaccagcaac gccaaccagg cggtgccgct
     9361 gctgttcaac ctcaccttcc cgtcgtggtt ccgggggttc gccttcgcgg cggtggcgat
     9421 cggggcgctg gtgccggccg cgatcatgtc gatcgcggcg gccaacctgt tcacccgcaa
     9481 cgtctaccgg gagttcttcc gacccgacgc cacccctgcc gaggaggcgg gcgtggcgcg
     9541 gctggtctcg ctggtggtca agctcggcgc gctggcgttc atcctgctcg tcaagcagga
     9601 cttcgccatc cagttccagc tcctcggcgg ggtctgggtc ctgcagacct tcccggcgat
     9661 cgtgctcggc ctgtacaccc gctggttcca ccgctgggcg ctgctgtgcg gctgggcggt
     9721 cggcatggcc agcggcacct ggatggcggc gacccagcac ttcaagcagt cgacggtgcc
     9781 cttcaacctc ttcggcatca agttcaccgc ctacgcggcg ctcgccgcct tcgtgctgaa
     9841 cctgctggtg gccatggtgc tcacgccgct gttcaacgcc atgcgggcgc cgagcggcag
     9901 cgacgagacc accccgcccg actacgaggt cgaggcggcc ccgccgatcc gcgtcggcac
     9961 cggagagccg tcgctctagt cgctgtccga gaacctgcca ccatgtgcgg gtgagcgatg
    10021 agcgtgaccc gcccgccggc cggcccccgg tcccgaccgt cgcggtcggt ctggtcgccc
    10081 gcgatcccag cgggcggctg ttgctggtgc ggcgcggcca cccgccctcg gagggcctgt
    10141 ggtcgctgcc gggcgggcgc gtcgaggccg gcgagacgct cgccgaggcg gccgcccggg
    10201 agttgcgcga ggagaccggc ctggcggccc gcatcggcac gatcgccggt gtggtcgagc
    10261 ggatcggcga gggctaccac tacgtgatcg ccgacctgtg ggccgagctg gacggcgaca
    10321 gcccggcgcc ggccgccgcc agcgacgccg ccgcggtccg cctggtcgag ctggaggcgc
    10381 tgcccggcct cgacctgacc cctggactgg cggagttcct ggaggggatc ggctgctggc
    10441 cgcctggcga gcacgccccc gggtaggtcc agcggctacg ctgtgcgggt atgcctgagc
    10501 ttcccgaggt tgagtccgtc cggcggcagc tcgaaccgga gctgcgcggc cgtcgcgtgg
    10561 tcgctgtctg ggccgaccgg cagcgccgct tccatcaggt cgagcggctc gcgggccata
    10621 cggtggaggc ggtgggccgt cgcggcaagt tcctcgtctg cccgctcgac ggcggcctgg
    10681 aactggtcat gcacctcggc atgaccggcg tgctggggat ccgccgggct ggcctggacg
    10741 gcgaggccgc cccctacacg cgcgcccggc tgtccctgga cgacggccgg gagctgacct
    10801 ttcaggacgt gcgccggttc ggcggcctgg cggtggtcga ggcgggcgcc tacgggacgg
    10861 tcctgccggc tctggcccgt ctcggccccg agccgctgtc ggacgcgttc gacccggcgc
    10921 gcttcgcccg cgacttggcc gccacccgcg caccggtcaa gccgtatctg ctcagccagc
    10981 ggccggtcgc cggggtcggc aacatctacg ccgacgaggc gctgtggctg gcgcggatcc
    11041 acccggcctc ccggagggtc gggcgggcgc gtgccctgct cctgcacgcc gccatccgcg
    11101 aggtcctcgc ccaggcgatc gaacgggagg gcaccacctt ccgcgactac cggatgctca
    11161 acggcgagag cggccgcaac gccaccttcc tgaccgccta cggccaggcc ggccgcccct
    11221 gcccgcgctg cggcacgccc ctccgcaaga tcaccctcgg cgggcgggga acgaccttct
    11281 gccccacctg ccagcgccac tgacccgacc agggaggatg gagacatgcc agaaggcagc
    11341 gaacccgagc tgtccccgcg ggcgtcggac gccgaacgca accagacggt cgaccagctg
    11401 cgcacggcgt ttgtcgaggg gcggctcaac gacgacgagt tcgaccagcg gatgcgcgcc
    11461 gccctgaccg cgcggaccca cgcggacctc aaccgcctcc tcaccgacct gccgcaggcg
    11521 actgtggtgg cggccccggc cacggcgacg ccctcgcccc tgatcggcgg gaccgcgccc
    11581 ggcgcgcccg ccacccactg ggccgtcgcg gtcatgagcg gcacccgccg gcacggccgc
    11641 tggaaggcgc cgcccgcgat caacgccgtc gccatcatgg gcggctgcga gctggacttc
    11701 cgcgccgcca ccttctccag tcagacgacc acggtgacgg tcgcctgtat catgggcggg
    11761 gtcgagatca ccgtgccgcc cggcatccgc gtggaatggc acggcttcgc cctcatgggc
    11821 ggcatggagg accgggtcga cgagaccgac ctgccagccg gggccccggt cctgatcgtc
    11881 cgcgggttcg tcatgatggg cggcgtcgag atccagacca aggcccccaa ggacaaggac
    11941 aacggccagc gccgcgccct gccctgaggt ccatgccgct accgcccggg gagcctgccc
    12001 cagaacagga tccgcttata cggatagggg taggggtgga ggtcgccaag gcgctccagc
    12061 aggcgttcgc ggtaggcggc caggaaccgc tcgtacagct cggcgggcag gtggcgctgg
    12121 taccaggtga gcagggtgcc cttcacccac tcgaccacgt cggcggggcc gggcaggtgg
    12181 tgcaggaaga cctgcagccg gacgtgctgc tccacgaagc cgagccggtc gagcgcgagc
    12241 gcgtaccagt cgggttcccg cacgttcacc gggcgcccgc caccgccgag cacctcccgg
    12301 aacggcgcct cgccggccag ttcccaggcg accacgtggg cgaggtggtc gtcgttggcc
    12361 ggaatctgga cggcgagctg ccccccgggg gcgagcgcgg cggcgaagcc ggtgagcaac
    12421 gcctcgtggt cgctgaccca ttgcagggcg gcgttggaga agagcacgtc ccagccgccc
    12481 ccagccccgg attcggcccg ggctctggcg ccaaaggctg cgatgtcgcc ggactcgaag
    12541 tggagaccgc cgcccgccgc tgcctccgtg cgcgccagca tggcggcgga gttgtcgatc
    12601 ccgaccgtct cgctggcccc gaggtgccgg tgcagctcgg cggtgagcgc cccggtgccg
    12661 caaccgaggt ccgccgcccg gccgccgggg gccggctcga ccagggcgag cagctcgaag
    12721 aagggcgcgt tgcgctcagc ggcgaagcgt tcgtactgcg ccggatccca ggtgtcccgc
    12781 atctccgtcc tccggggtga tagcgcgtgt cggcgccgca actgtactga cgcgcctcaa
    12841 ggaggcgggc
//
LOCUS       DASWDC010000187         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1109497, whole genome shotgun sequence.
ACCESSION   DASWDC010000187 DASWDC010000000
VERSION     DASWDC010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109497"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VF995_06025"
     CDS             <1..660
                     /locus_tag="VF995_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX9343155.1"
                     /translation="NAPAENPLVDASADDVFHHGNFLTATLTLALDKARAALFQAAAL
                     SAARCAALMEPELTGLRPFLADGPATSSGLMMLEYVAHSALADLRLHATPATLGCAVL
                     SRGVEEHASFATQAAWRLTDSRPAYETVLACELLAATRAVRLNANAAVPAALRPVLTC
                     TGTALDPRTEDRPLDADLATATTLLPELAEFAEPTRAAAARSPEHRSGSRPREAVSRR
                     S"
     gene            complement(644..1288)
                     /locus_tag="VF995_06030"
     CDS             complement(644..1288)
                     /locus_tag="VF995_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004202046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9343156.1"
                     /translation="MKVLLFGASGAIGSQIATELLGRGHQVTGATRHGPAGDLSNPNL
                     TVTTGDLTDPGAVARLAPGHDAVASAIGPIRGSSDDPQELVAAAKALIDGLRQAGVPR
                     LVVVGGAGSLEAAPGVRVIDTPDFPPAWKPTAQAHADALEVYRTITDLDWTYISPAAL
                     IGPGERTGQFRIGGDQLLTDAEGNSRISIPDYAIAFVDELEQGDAIRRRITIAY"
     gene            complement(1329..1832)
                     /locus_tag="VF995_06035"
     CDS             complement(1329..1832)
                     /locus_tag="VF995_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9343157.1"
                     /translation="MPITRLNHAVLFVRDLRRSASFYQEVLGFRAVNMTPEGFGGAVF
                     LQAPGSTNDHDLGLFEIGSAAADSAAGRHTVGLYHLAWEVDTLAELERLAGLLRERGS
                     LVGASDHGTTKSLYAKDPDGLEFEVAWIVPAELLDQAALAARKRIGPLDLDRAKERYG
                     ATTPGGI"
     gene            1926..2384
                     /locus_tag="VF995_06040"
     CDS             1926..2384
                     /locus_tag="VF995_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9343158.1"
                     /translation="MNQPNWLDPDELRAWRTFVEATQLLFDRLDRQLQQDSGLSHADY
                     ELLIRLSEAADQRMRMSELAEQTLFSRSRLSHAIARIEQAGWVRREGCPSDKRGTFAV
                     LTPEGRQVLEAAAPGHVAAVRHHLFDQLTPVQRRQLQQIANRIREQLGHP"
BASE COUNT          390 a         1046 c          940 g          423 t
ORIGIN      
        1 aacgcccccg ccgagaaccc gctggtcgac gcgtccgccg atgacgtgtt ccaccacggc
       61 aacttcctca ccgccaccct caccctggcg ctcgacaagg cacgcgccgc gctcttccag
      121 gccgccgccc tgtcagccgc ccgctgcgcc gcgctgatgg agcccgagct caccggcctc
      181 cggccattcc tcgccgacgg cccggcgacc agctcgggcc tgatgatgct cgagtacgtc
      241 gcccactccg ccctcgccga cctgcgcctc cacgccaccc ccgccaccct cggctgcgcc
      301 gtcctctccc gcggcgtcga ggagcacgcc agcttcgcca cccaagccgc ctggcgcctg
      361 accgactcgc gccccgccta cgagaccgta ctcgcctgcg agctgctcgc cgccacccgc
      421 gccgtgcgcc tcaatgccaa tgccgccgtc ccggccgccc tgcgcccggt cctgacatgc
      481 accggcacag cgctcgaccc acgcaccgag gaccgcccgc tcgacgccga cctggccacg
      541 gccaccacgc tgctcccgga gctggccgag ttcgccgagc ccacccgcgc cgccgcggcg
      601 cggagcccgg aacaccggtc agggtcgagg ccgcgggagg ccgttagtag gcgatcgtga
      661 tccggcggcg gatggcatcg ccctgctcca gctcgtccac gaaggcgatg gcgtagtccg
      721 ggatcgagat gcggctgttg ccctccgcgt cggtgagcag ctggtcgccg ccgatgcgga
      781 actggcccgt ccgctcgccc ggaccgatca gcgcggcagg ggagatgtag gtccagtcga
      841 ggtcggtgat ggtccggtag acctcgagcg cgtcggcgtg ggcctgtgcc gtcggcttcc
      901 aggccggcgg gaagtccggg gtgtcgatca cccgcacgcc gggggcggcc tcgaggctgc
      961 ccgccccgcc gaccaccacc aggcggggca cgcccgcctg gcgcaacccg tcgatcagcg
     1021 ctttggcggc ggcgaccagc tcctgcgggt cgtcgcttga gccgcggatc gggccgatgg
     1081 ccgaggcgac cgcgtcatgg ccaggagcga gccgcgcgac ggcgcccggg tcggtgaggt
     1141 ccccggttgt caccgtcagg ttcgggttcg acaggtcgcc ggccggaccg tggcgcgtcg
     1201 cgccggtgac ctggtggccg cggccgagca gttcggtggc gatctggctg ccgatggcgc
     1261 cgctggcgcc gaacaacaga accttcatcg atgggctcct tcgggatgga ggtgggtggg
     1321 tcgaacgctc agatcccgcc cggggtggtg gcgccgtagc gctccttcgc gcggtccagg
     1381 tcgagcgggc cgatgcgctt gcgggcggcg agcgcggcct ggtcgagcag ttcggcgggg
     1441 acgatccagg cgacctcgaa ctcgaggccg tcggggtcct tggcgtagag gctcttggtg
     1501 gtgccgtggt cggaggcgcc gaccagggag ccgcgctcgc gcagcagccc cgccagccgc
     1561 tccagctcgg cgagggtgtc gacctcccag gcgaggtggt agaggccgac ggtgtggcgt
     1621 ccggccgcgg agtcggcggc ggccgagccg atctcgaaca ggccgaggtc gtggtcgttg
     1681 gtcgacccgg gtgcctgcag gaacaccgcg ccgccgaagc cctccggggt catgttgacc
     1741 gcgcggaagc cgaggacctc ctggtagaag gaggcgctgc gccgcaggtc gcggacgaac
     1801 agcaccgcgt ggttcaggcg ggtgatcggc atgatttcct ccgttggtgg ttgaagcctc
     1861 aacggtgacc ggcagcctat cgccgattag ttgatctgtc aattaaatcc cgttacagtg
     1921 ggtgggtgaa ccaacccaac tggctggacc ccgacgagct gcgggcctgg cgcacctttg
     1981 tcgaggccac acagctgttg ttcgaccggc tcgaccggca gctgcagcag gactcaggcc
     2041 tctcccacgc cgactacgag ctgctgattc gcctttccga ggccgctgac cagcgcatgc
     2101 gcatgtcgga gctggccgag cagacgctgt tctcgcgcag ccgcctctcc cacgccatcg
     2161 cccgtatcga gcaggccggc tgggtccgcc gcgagggctg cccaagcgac aaacggggca
     2221 ccttcgccgt gctcaccccc gaaggccgcc aggtcctgga ggccgccgcc cccgggcatg
     2281 ttgccgctgt ccgccaccac ctgttcgacc agctcacccc tgtccagcgg cgccagctcc
     2341 agcagatcgc caaccgcatc cgcgagcagc tcggccaccc ttgagtcccc accaccgcgt
     2401 ctggttctgc tggaacattc caagcgggcg acaaaccgcc gaacggcgca tgtcagccac
     2461 cggacagccg cgcagacggc tcgttgtgct ggttgcgttg acatcacaag ttgaacgaat
     2521 gcggccttgc ttgctgtgct tgacatcaca agttaaacgg tcatattgtc acattccggt
     2581 ttgttccagt ctgatccgct ctgatcccat gcctgccgct gcgcacggaa cagaagggac
     2641 ggtacgacca tgcagctgcc acggccgagg ttcccctccc accttcccgc ctccgccctc
     2701 cggacgtccc tgcgcgccgt cacagcgctc gggctgctcc tggccgcggt ggtcactggc
     2761 ggcgccagcg tcagcgttgg ctcaccggcc gcctccggc
//
LOCUS       DASWDC010000188         7622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_106952, whole genome shotgun sequence.
ACCESSION   DASWDC010000188 DASWDC010000000
VERSION     DASWDC010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106952"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="VF995_06045"
     CDS             complement(<1..106)
                     /locus_tag="VF995_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX9343159.1"
                     /translation="MPISKATLNRHVFVCGATGGGKSQTVRALLESLHD"
     gene            125..2242
                     /locus_tag="VF995_06050"
     CDS             125..2242
                     /locus_tag="VF995_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9343160.1"
                     /translation="MELEEALGAEFDEGDFGASPFRAPSELLAAIARPPTRELPGIRM
                     VAASTFDVTPESTGDLTLGEVLDQGLNPAGPMPISKATLNRHVFVCGATGGGKSQTVR
                     ALLESLHDAGVPWLVIEPAKAEYGRMAGRLKGRGEVLVIKPGQPDQIPGSLNPLEPEP
                     GFPLQTHIDLVRALFLAAFEAEEPLPQVLSYALSRCYEEMGWELALGESKIEGVTPKY
                     PTLGELQRVARDVVEKIGYGDEVEDNVKGFIDVRIGSLRLGTPGRFFEGGHPLDLAAL
                     LRRNVVLELEDIGNDQDKAFFMGTVVIRLVEHLRMRYGTTEGDVGLRHMTVVEEAHRL
                     LKNVEEGSPAAHAVELFAGLLAEIRAYGEGILVAEQIPSKLLTDVIKNSALKIVHRLP
                     ANDDREFVGATMNLTPEQADYVVTLPPGQAAVFADAMDRPLLVRMPLGEQREQVAGAL
                     HTPPLLRSRSACCGKACQRRPCTLRELTKAKRSAEDPRLTLWIELLMASHLVGETEPA
                     PDPVWLAQLRDSVEARQLDCAIGQLAQAAIDSRYAGLAAFYQPEELAKHVADRVRAWL
                     FERQSRRKTTCDGSEITWYAGKYRWIDVLRALQEDHGDRMQPHPKTDEWTARGIRLDG
                     PTWAEQLSQLQSHPLVWGSSYKTIWGDDDPCAVERAAEALSFAEDLVERIEAATGFLK
                     VECPWPAARLYPRLWAKRKEGPS"
     gene            2242..2436
                     /locus_tag="VF995_06055"
     CDS             2242..2436
                     /locus_tag="VF995_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343161.1"
                     /translation="MGANDKKGAERAGGALQGLGPKDDLGQLDLVSQGGTAGDVGGDN
                     TVNNVNVDLIKTGDISAIPM"
     gene            2456..2995
                     /locus_tag="VF995_06060"
     CDS             2456..2995
                     /locus_tag="VF995_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343162.1"
                     /translation="MAEADRKKKGKGRDRGGRRSDLGVPEPDVLSSEVLNLHWEGKDG
                     QMLDVRRAGGEGPLHMGVNQDKRGVSINVNGETEQQPDQLKEAADRFRGNVVDGFIDH
                     DRNIFGKERLVGEGVLADGRKVKLDGGRDIFGGAHVNEQWGPDASGTGEGRGRDAGGL
                     PPPEAPSAPDAPEPPLPPK"
     gene            complement(3009..6419)
                     /locus_tag="VF995_06065"
     CDS             complement(3009..6419)
                     /locus_tag="VF995_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX9343163.1"
                     /translation="MGRRVSSSQSQRARPRSGETLRALAALLGILALTVGVPLALWQA
                     VGWPLPHALPTLGELRDVLSRTAIPEEFFPKAVAVFAWLVWAQFVACIVVEAWSALRG
                     RLSRQVPGAGWGTQRLASNLVAAALLLLPSQGLSVVAFASTPQRPPAVVRTVAPAAGG
                     TPGLLADQATGTASTAAGGATGTGTNGTAASAGSGQAARDTAKAPVRYEVHRRDTLWD
                     IAERHLGDPLRWPEIFELNKGRLQPDGRELKQAHWIFPGWVLLLPPDATGVPKPGTGG
                     QSQPGSGTTTPQHQTPTPTTATTTTSPQPNAPAPTTTSPPNGTHTQTSHPTTTPSTAP
                     SGNGTAPSGLPVPGSPGAATPTTTLPATTTTQPSGGGGGGVAPAPSDLTPTTAPPGTP
                     RTTTSPEASKEPGGTAASQHWPVDNLSTNLQHAGLLAAGLLAVLGVFRVIQQRRRRFR
                     RQIAMPDEGLAPIEAALRSGEAPDAAELVDLGLRTMAAVVQQEGMELPEVVGVLVSNE
                     MMEILLATEAPAAPEPFVLSYSRRRWGLPVEVPLERLREAAGDAVSPVPALVTLGRTS
                     NGLLLVNLESPGLTALGGDEDAARAVLTSFAVELATCRWADYIELVLVGFGGELQRLE
                     RARTVASVREMLPWLEHKADDVADLLGETGARSVLAGRIAGTTPDGWVPAVVLCAERP
                     TAEEVTRLVALTAVPSRSPVAAVIAAGEVSGARWTLDLPEAAGELLHLPQFGVDVVPQ
                     RLDPDDYDGIARLLDTTTRDDVPIPDPMREPARRPFRRPRPAPVPIDDDYDDKLDEDG
                     IETETDEGSDVTSTEPAEHDEVAVPTAAAASSEPAESEPAESADGAPATGEPEPEPGA
                     EAEPRKTPHLHELRLSSRVIPDPEMDDESEVEVRVLGKVEIGGIDRVERGKSEELIVF
                     LALHPDGVDGDQLSEALWPGRPPAKGTLNTTTAVARAYLGMTPDGEPRLPHARNGIYR
                     LDPSVGLDWTRFHTLAERGYGQGEDGAADLRRALELVRGRTFQGAKPRSYGWAQLDEV
                     PVMESSIVDAADHLAGLLLHAGDHGGCQWAARRGLVTAPYDERLYRKLMLAADAAGNP
                     AGVEAVMDELLLRLDEENLEPYDTLHEETRALYERLTRHRAARKTNAG"
     gene            complement(6419..6916)
                     /locus_tag="VF995_06070"
     CDS             complement(6419..6916)
                     /locus_tag="VF995_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX9343164.1"
                     /translation="MDSDSVDVESYRNPAPHRLAGRGLDHDRVEGEAGAIAVMVAILG
                     VSLMLVVGLVFEGGVAIAAKRRAMNVAEQAARAGAEQLDLNAVREGTGFRIDPGAAVA
                     AADAYLAGAGFSGSVGVSGDTVTVSGVAWSSQDPMLGTLGVHFSGTVAAAHARNLHGV
                     VTVEN"
     gene            complement(6922..7380)
                     /locus_tag="VF995_06075"
     CDS             complement(6922..7380)
                     /locus_tag="VF995_06075"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX9343165.1"
                     /translation="MSATGAIRSRFARAAGGGNGEDGSAVEAAIVAPALIMLILLVVL
                     AGRVTSAKMRVEEAARDAARAASLASDASSASSAASSAAQASLGNAGITCQSFSVSSF
                     SWNWSPGVGGTADVTVQCTTPLSDLAPLPLPGSKTLSGSFQVPIDPFRSQ"
     gene            complement(7377..>7622)
                     /locus_tag="VF995_06080"
     CDS             complement(7377..>7622)
                     /locus_tag="VF995_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012300383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343166.1"
                     /translation="DRAARLFQATDADGTTRANQALQALNAGGILTSPQVNVTRAGGN
                     VTVTVAGGVVSLLPGVHLTIRPQVSEGPVEVFKRPGQ"
BASE COUNT         1043 a         2689 c         2854 g         1036 t
ORIGIN      
        1 cgtcgtggag ggactccagc agcgcccgca cggtctggga cttgccgccg ccggtggcgc
       61 cgcagacgaa cacgtgccgg ttcaaggttg cctttgagat cggcatcggc ccggccgcaa
      121 ggcggtggaa ctggaggagg cgctcggcgc cgagttcgac gagggcgact tcggcgcttc
      181 gccgttccgc gcgcccagcg agctgctggc cgcgatcgcg cgcccgccca cccgcgagct
      241 gcccggcatc cgcatggtcg ccgcctccac cttcgacgtc accccggaat ccactggcga
      301 cctgaccctt ggcgaggtcc tggaccaggg cctcaacccg gccgggccga tgccgatctc
      361 aaaggcaacc ttgaaccggc acgtgttcgt ctgcggcgcc accggcggcg gcaagtccca
      421 gaccgtgcgg gcgctgctgg agtccctcca cgacgccggt gtgccgtggt tggtgatcga
      481 accggccaag gccgagtacg gccgcatggc cggccgcctc aagggccgcg gcgaggtgct
      541 ggtgatcaag ccaggccagc ccgaccagat ccccggctcc ctgaacccgc tggaacccga
      601 gcccggcttc ccgctgcaga cccacatcga cctggtccgg gcgctgtttt tggccgcgtt
      661 cgaggcggag gagccgctgc cgcaggtgct gtcctacgcg ctctcccgct gctacgagga
      721 gatgggctgg gagctggcac tgggcgagtc caagatcgag ggggtcacgc cgaagtaccc
      781 gacccttggc gaactgcagc gggtcgcccg cgacgtggtc gagaagatcg gctacggcga
      841 cgaggtcgaa gacaacgtca agggcttcat cgacgtgcgc atcggttcgc tgcgcctggg
      901 cacgcccgga cgcttcttcg agggcggcca cccgctggac ctggccgcgc tgctgcgccg
      961 caacgtcgtg ctggagctgg aggacatcgg caacgaccag gacaaggcct tcttcatggg
     1021 caccgtggtg attcgtctgg tggagcacct gcgcatgcgc tacggcacca ccgagggcga
     1081 cgtcgggctg cgccacatga ccgtggtgga ggaggcccac cgcctgctca agaacgtgga
     1141 ggagggcagc cccgccgcgc acgccgtgga gctgttcgcg gggctgttgg ccgagatccg
     1201 cgcctacggc gaggggatcc tggtggccga gcagatcccc agcaagctgc tgaccgacgt
     1261 gatcaagaac agcgccctga agatcgtgca ccgcctgccc gccaacgacg accgcgagtt
     1321 cgtcggggcc accatgaacc tcacccccga gcaggccgac tacgtggtca ccctgccgcc
     1381 ggggcaggcg gcggtgttcg ccgacgccat ggaccggccg ctgctggtgc gcatgcccct
     1441 tggcgagcag cgcgagcagg tggcaggggc gctgcacacc ccgccgctgc tgcgctcccg
     1501 cagtgcctgc tgcggcaagg cctgccagcg gcgcccctgc acgctgcggg agctgaccaa
     1561 ggccaagcgc tccgccgagg acccccgcct gaccctgtgg atcgagctgc tgatggcctc
     1621 ccacctggtc ggggagaccg agcccgcccc cgacccggtg tggctcgccc agctccgcga
     1681 cagcgtcgag gcgcggcagc tcgactgcgc catcgggcag ctcgcccagg ccgccatcga
     1741 ctcccgctac gccggcctgg ccgccttcta ccagccggag gagctggcca agcacgtggc
     1801 cgaccgggtc cgcgcgtggc tgttcgagcg ccagagccgc cgcaagacca cctgcgacgg
     1861 cagcgagatc acctggtacg cgggcaagta ccgctggatc gacgtgctcc gcgccctcca
     1921 ggaggaccac ggcgaccgca tgcagcccca cccgaagaca gacgagtgga ccgcccgcgg
     1981 catccgcctg gacggcccca cctgggccga gcagctctcc cagctgcagt cccacccgct
     2041 cgtgtggggc tcgtcctaca agaccatctg gggcgacgac gacccctgcg cggtcgaacg
     2101 cgccgccgag gccctcagct tcgccgagga cctggtcgaa cggatcgaag ccgccaccgg
     2161 cttcctgaaa gtcgaatgcc cctggcccgc cgcgcgcctg tacccgcgcc tatgggccaa
     2221 gcgcaaggag ggtccgagct gatgggcgca aacgacaaga agggcgcgga gagggccggc
     2281 ggggcgctgc agggtctggg ccccaaagac gacttgggcc agctggacct ggtctcgcag
     2341 ggcgggacgg ccggcgacgt cggcggcgac aacaccgtca acaacgtgaa tgtcgacctg
     2401 atcaagaccg gcgacatctc cgcgatcccg atgtaggcag aagggcgcat cctccatggc
     2461 tgaggccgac cggaagaaga aggggaaggg cagagatagg ggcgggcgtc ggtcggacct
     2521 gggcgtgcct gagcccgacg ttctctcgtc tgaggttctc aacctgcatt gggagggcaa
     2581 ggacggccag atgctcgacg tgaggcgcgc gggcggcgag ggtcccctcc acatgggcgt
     2641 caaccaggac aagcgcggag tcagcatcaa cgtcaacggc gaaaccgagc agcagcccga
     2701 tcagctcaag gaggccgctg atcggtttcg cgggaacgtg gtggacggct tcatcgacca
     2761 cgatcgcaac atctttggta aggaacggct cgtgggcgaa ggcgtcctgg cagacggcag
     2821 gaaggtcaag ctggatggtg gcagggacat ctttggcgga gcccacgtca acgagcaatg
     2881 gggtcccgac gctagcggta ccggcgaggg caggggcagg gacgcaggag gtcttcctcc
     2941 ccccgaggct ccctctgcgc ccgatgcgcc cgagcctcct ctgcctccca agtgaggtgt
     3001 aggtcccctt accccgcgtt ggtcttgcgg gcggcgcggt ggcgggtgag gcgctcgtag
     3061 agggcgcggg tctcctcgtg gagggtgtcg tagggctcca ggttctcttc gtcgaggcgg
     3121 aggagcagct cgtccatgac tgcttcgacg ccggcggggt tgccggcggc gtcggcggcg
     3181 agcatcagct tgcggtacag gcgctcgtcg tagggggcgg tcacgaggcc ccggcgggcg
     3241 gcccattggc agccgccgtg gtcgccggca tggagcagca ggccggcgag gtggtcggcg
     3301 gcgtcgacga tcgatgattc catcaccggg acctcgtcga gctgggccca gccgtaggag
     3361 cgcggcttgg cgccttggaa tgtgcggccg cggaccagct ccagggcgcg gcgcaggtcg
     3421 gcggcgccgt cctcgccctg gccgtagccg cgctcggcca gcgtgtggaa gcgggtccag
     3481 tccagcccca ctgacgggtc cagccggtag atgccgttgc gggcgtgggg caggcgcggc
     3541 tcgccgtcgg gggtcatgcc gaggtaggcg cgggccaccg cggtggtggt gttgagggtg
     3601 cccttggccg gcgggcggcc gggccacagc gcctcggaga gctggtcgcc gtcgacgccg
     3661 tccgggtgga gcgccaggaa cacgatcagt tcctcggact tgccgcgctc gacccggtcg
     3721 atgccgccga tctcgacctt gccgagcacg cgcacctcga cctcggactc gtcgtccatc
     3781 tccgggtcgg ggatcacccg gctgctgagg cgcagctcgt gcaggtgcgg cgtcttgcgc
     3841 ggctcggcct cggctccggg ctccggctcc ggctcgccgg tcgcgggcgc gccgtctgcg
     3901 ctctctgcgg gctcgctctc tgcgggctcg ctgctcgcgg cggccgccgt gggcacggca
     3961 acctcgtcgt gctctgcggg ctctgtgctg gtcacatcag acccttcatc ggtctcggtc
     4021 tcgatgccgt cctcatcgag cttgtcgtcg tagtcgtcgt cgatgggaac tggcgctggc
     4081 cgtggccggc ggaagggccg gcgggccggc tcccgcatgg gatccgggat ggggacgtcg
     4141 tcccgcgtgg tggtgtcgag cagccgggcg atcccgtcgt agtcgtccgg gtcgagccgc
     4201 tgcggcacca cgtcgacgcc gaactggggc aggtggagga gctcgccagc ggcctcggga
     4261 aggtccaggg tccagcgggc cccggagacc tctccggcgg cgatcaccgc ggcgaccggt
     4321 gagcgggagg gcacggcggt gagcgcgacc agccgggtga cctcctcggc ggtgggccgt
     4381 tccgcgcaca gcaccaccgc cgggacccag ccgtccgggg tggtgccggc gatccggccg
     4441 gccagcaccg agcgggcgcc ggtctcgccg agcaggtcgg cgacgtcgtc ggccttgtgc
     4501 tccagccacg gcagcatctc ccggacgctg gcgacggtgc gggcccgctc cagccgctgc
     4561 agctcgccgc cgaagccgac caggaccagc tcgatgtagt cggcccagcg gcaggtggcc
     4621 agctccaccg cgaacgaggt gagcaccgcc ctggccgcgt cctcgtcgcc gccgagggcg
     4681 gtcaggccgg gagactccag gttgaccagc agcagcccgt tgctggtccg cccgagcgtc
     4741 accagcgccg gcaccggcga gacagcgtcg ccggccgcct cccggagccg ctccagcggc
     4801 acctcgaccg gcaggcccca gcggcggcgg gagtacgaca gcacgaacgg ctcgggcgcg
     4861 gccggcgcct cggtggccag caggatctcc atcatctcgt tgctgaccag cacgccgacg
     4921 acctcgggca gctccatgcc ctcctgctgc accaccgccg ccatcgtccg caggccgagg
     4981 tcgaccagct cggccgcgtc cggggcctca cccgagcgca gcgcggcctc gatcggagcc
     5041 aggccttcgt ccggcatggc gatctgccgc cggaagcgcc gccgccgctg ctggattacc
     5101 cggaacacgc cgagcacggc caagagcccg gcagcgagca gcccggcgtg ctgcaggttc
     5161 gtgctcaggt tgtcgaccgg ccagtgctgc gaggcagcgg tgccgcccgg ctccttggag
     5221 gcctccggtg acgtcgtggt ccgcggggtg ccgggagggg ccgtggtggg ggtcaggtcg
     5281 gacggcgccg gcgcgacccc gccgccaccg ccaccagagg gctgggtcgt cgtggtggcc
     5341 ggtagggtcg tggtcggcgt ggcggccccc gggctcccag gcaccggcag gccggagggg
     5401 gcggtgccgt tgccgctggg ggcggtgctc ggcgtcgtgg tcgggtggga ggtctgggta
     5461 tgggtgccgt ttggtgggct ggtcgtggtc ggcgcgggcg cgttcggctg cggcgacgtg
     5521 gtagtcgtgg ccgtcgtggg tgtcggagtc tggtgctgcg gtgtggtggt tccggaccca
     5581 ggctggctct ggccgccggt acccggcttg ggcaccccgg tcgcgtcggg gggcagcagc
     5641 aggacccagc cagggaagat ccagtgcgcc tgcttcagct cacggccgtc tggctgcagg
     5701 cggcccttgt tcagctcgaa gatctccggc cagcgcagcg ggtcgccgag gtgccgctcg
     5761 gcgatgtccc acagcgtgtc gcggcggtgc acctcgtagc gcacgggtgc cttggcggtg
     5821 tccctggccg cctgcccgct gccggcgctg gcggcggtgc cgttggtccc ggtcccggtc
     5881 gcaccccccg cggccgtgga cgccgtgccg gtggcctggt cagcgagcag gcccggggtg
     5941 ccgccggccg ctggcgcgac ggtgcggacc acggctgggg gccgctgcgg cgtgctcgcg
     6001 aaggcgacca cgctgagccc ctggctcggc agcagcagca gcgccgccgc caccagattg
     6061 gaggccagcc gctgggtgcc ccagccggcg ccgggcacct gccgcgagag ccgcccgcgc
     6121 agtgccgacc aggcctccac cacgatgcag gcaacgaact gcgcccacac cagccaggcg
     6181 aacaccgcca cggccttggg gaagaactcc tcggggatcg ccgtgcgcga cagcacgtcg
     6241 cgcagctcgc ccagggtcgg tagggcgtgc ggcagcggcc agcccaccgc ctgccacaac
     6301 gccagcggca ccccgaccgt cagcgcgagg atgccgagga gcgcggcaag ggcgcggagg
     6361 gtctccccgg accgcggcct ggcccgctgg ctctggctgg aggacacccg ccggcccatc
     6421 agttctccac cgtgacgacg ccgtgcaggt tgcgggcgtg cgccgccgcg accgtgccgc
     6481 tgaagtggac gccgagggtg ccgagcatcg ggtcctggga cgaccaggcc acgccgctga
     6541 cggtcacggt gtcgccgctg acccccaccg agccgctgaa gccggcgccg gccaggtagg
     6601 cgtcggcggc ggcgacggcg gcgccggggt cgatccggaa gccggtgccc tcgcggaccg
     6661 cgttgaggtc gagctgctcg gcgcccgccc tggcggcctg ctcggcgacg ttcatggcgc
     6721 ggcgcttggc ggcgatcgcg acgccgccct cgaagaccag gccgaccacc agcatcagcg
     6781 acacaccgag gatggcgacc atcaccgcga tcgcgccggc ctcgccctcc acgcggtcgt
     6841 ggtcgaggcc acggccggcg aggcggtgcg gggccgggtt ccggtacgac tcgacgtcga
     6901 cgctgtcgct gtccatcgcg atcactggct cctgaacggg tcgatgggga cctggaagct
     6961 gccggaaagg gtcttggagc cgggcagcgg gagcggcgcg aggtcgctca gcggggtggt
     7021 gcactgcacg gtcacgtcgg cggtgccgcc gacgccgggg ctccagttcc agctgaagct
     7081 ggacaccgag aacgactggc aggtgatccc ggcgttgccg agcgacgcct gcgccgccga
     7141 actggccgcc gacgacgcgg aactggcgtc ggaggcgagg gaggcggcgc gggccgcgtc
     7201 gcgggcggcc tcctcgaccc gcatcttggc gctggtgacc ctgccggcga gcaccaccag
     7261 caggatcagc atgatgaggg cgggcgcgac gatcgccgcc tcgaccgccg agccgtcctc
     7321 gccgttgccc ccgcccgcgg cgcgggcgaa cctggagcgg atcgcgcccg tggcgctcac
     7381 tgaccaggcc tcttgaacac ttcgaccggc ccttcggaga cctgcggccg gatcgtcagg
     7441 tgcaccccgg ggagcagcga gaccaccccg ccggccaccg tgacggtcac gttgccaccg
     7501 gctcgggtca cgttgacctg cgggctggtc aggatcccgc cggcgttcag ggcctggagc
     7561 gcctggttgg cgcgggttgt gccgtcggcg tcggtggcct ggaagagacg ggcggcgcga
     7621 tc
//
LOCUS       DASWDC010000189         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_546306, whole genome shotgun sequence.
ACCESSION   DASWDC010000189 DASWDC010000000
VERSION     DASWDC010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546306"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VF995_06085"
     CDS             <1..199
                     /locus_tag="VF995_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX9343167.1"
                     /translation="TPEMVKPGAAVVDVGNHRVDGALVGDVDPSVAEVAGALTPVPGG
                     VGPMTVAMLLANTVAAAAGRV"
     gene            204..1130
                     /locus_tag="VF995_06090"
     CDS             204..1130
                     /locus_tag="VF995_06090"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX9343168.1"
                     /translation="MADQDLGRLAQLLDDGAFVVTGELVPPRSPDPQAVRRVARRLRG
                     VVDAVNVTDNATARVGMSPLAAGALVAAEGLEPVLQLTCRDRNRLALAADLLGAHALG
                     IRALLCLSGDPMEVGPFAKEGAKAVFDLDAIGLARLAHRLGRGVGPDGGTVSPPAPFL
                     VGVAEAPGADPSKGARLAAKQIAGARFVQTQPVYDPPAFAAWLGYLAERELLDRVAIL
                     AGVLPPRSAAQLERFAALPGWSVPAATIERMRAARDQPAEGVAIAAEVIAALRALPSP
                     GIRGIHLMGIGPDSGIPEVIEQAGLLPRPAPA"
     gene            complement(1162..2289)
                     /locus_tag="VF995_06095"
     CDS             complement(1162..2289)
                     /locus_tag="VF995_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX9343169.1"
                     /translation="MAESTGKGLEGVVAARTKISDIDGQRGKLFYAGYDIADLAEHAS
                     FEETTYLLHHLDLPTRAQLSDLNGQLRASQRLDDFLVRLIAALGDDTSPMTVLRTVVS
                     AAAAHDPDEGDESDEANLRKASRLVAITPQIIAGFHRLRSGGDAIEPREDLSLAGNFL
                     YALTGKVPDVALARDFDTVLVLYADHTMNASTFGARVAAATLADIYAAATAAIATLQG
                     PLHGGAIEQVRYMLDEIGTPDRAAGWVRERLGRKQKIMGFGHRVYKTWDPRARVLRGM
                     GERLGQQVGDTHWYEISAQIQQTVMDEKGLYPNVDFYTASVYSALGIPPDLYTTLFAA
                     SRMAGWTAHIREQYADNRLIRPDSEYIGPGPRTYTPIDQRG"
     gene            2461..>2579
                     /locus_tag="VF995_06100"
     CDS             2461..>2579
                     /locus_tag="VF995_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX9343170.1"
                     /translation="MAHRVTFIPGDGVGPELSEATRRVLEGTGVQFDWDVQEAG"
BASE COUNT          315 a          909 c          965 g          390 t
ORIGIN      
        1 cactccggag atggtcaagc ccggcgcggc cgtggtcgac gtcggcaacc accgcgtcga
       61 tggcgccctg gtcggcgacg tcgacccgtc cgttgccgag gtcgccggcg ccctcacccc
      121 ggtccccggc ggggtcggcc cgatgaccgt ggcgatgctg ctggccaaca ccgtcgccgc
      181 ggccgccgga cgggtctgag gcgatggctg accaggacct cggcaggctc gcgcagctgc
      241 tggacgacgg cgccttcgtg gtcaccggcg agctggtgcc gccccgctcc ccagacccgc
      301 aggcggtacg gcgggtggcg cggcgcctgc gcggggtggt cgacgcggtc aacgtgaccg
      361 acaacgccac cgcccgggtc ggcatgtcgc cgctggcggc gggggcgctg gtggcggccg
      421 agggcctgga gccggtgctg cagctcacct gccgcgaccg caaccgcctg gcgctggccg
      481 ccgacctgct cggcgcccac gccctcggca tccgcgcgct gctgtgcctg tccggcgacc
      541 cgatggaggt cgggccgttc gccaaggaag gcgccaaggc cgtgttcgac ctggacgcga
      601 tcggcctggc ccggctcgcc caccggctcg gccggggcgt cgggccggac ggcggcaccg
      661 tcagcccacc cgcgcccttc ctggtcggcg tggccgaagc gcccggcgcg gacccgtcca
      721 agggcgcccg cctggccgcc aagcagatcg ccggtgcccg cttcgtccag acccagccgg
      781 tctatgaccc gcccgcgttc gccgcctggc tcggctacct cgccgagcgg gagctgctcg
      841 accgggtcgc gatcctcgcc ggggtgctgc cgccccgctc ggcggcgcag ctggagcgct
      901 tcgccgccct ccccggctgg tcggtcccag ccgcgaccat cgaacggatg cgcgccgccc
      961 gcgaccagcc cgccgagggt gtcgccatcg ccgccgaggt gatcgccgcc ctccgcgccc
     1021 tccccagccc cggcatccgc ggcatccacc tgatgggcat cggccccgac agcggcatcc
     1081 ccgaggtcat cgagcaggcc ggcctgctgc cccgcccagc gcccgcctag cggaggacca
     1141 cccgagcgcg caattggccg ctcagccgcg ctggtcgatg ggggtgtagg tgcgcggccc
     1201 ggggccgatg tactcggagt cggggcggat caggcggttg tcggcgtact gctcgcggat
     1261 gtgggcggtc cagccggcca tgcgggaggc cgcgaacagg gtggtgtaga ggtcgggcgg
     1321 gatgcccagg gccgagtaga cgctggcggt gtagaagtcg acgttggggt acaggccctt
     1381 ctcgtccatc accgtctgct ggatctgggc ggagatctcg taccagtggg tgtcaccgac
     1441 ctgctggccg aggcgttcgc ccatgccgcg caggacgcgg gcgcgggggt cccaggtctt
     1501 gtagacgcgg tggccgaagc ccatgatctt ctgcttgcgg ccgaggcgtt ccctgaccca
     1561 gccggcggcg cggtccgggg tgccgatctc gtcgagcatg tagcggacct gctcgatggc
     1621 gccgccgtgg agggggccct gcagggtggc gatcgcggcg gtggcggcgg cgtagatgtc
     1681 ggccagggtc gcggcggcga ccctggcgcc gaaggtcgag gcattcatgg tgtggtcggc
     1741 gtacagcacc agcacggtgt cgaagtcgcg ggcgagcgcc acgtcgggga ccttgccggt
     1801 gagggcgtag aggaagttgc cggccagcga caggtcctcg cgcggctcga tcgcgtcgcc
     1861 gccgctgcgc aggcgatgga agccggcgat gatctgcggg gtgatcgcca ccagccggga
     1921 ggcctttcgc aggttcgcct cgtcagactc gtcgccttcg tccgggtcgt gtgccgcggc
     1981 ggcggagacc accgtgcgca gcaccgtcat cgggctggtg tcgtcgccga gggcggcgat
     2041 cagccgcacc aggaagtcgt ccagccgctg gctggcccgc agttgcccgt tcaggtcgga
     2101 aagctgcgcg cgggtgggca ggtccaggtg gtgcagcagg taggtggtct cctcgaagga
     2161 ggcatgttcg gcgaggtcgg cgatgtcgta gccggcatag aagagcttgc cccgctgtcc
     2221 gtcgatatcg gagatcttcg tccgggcggc gacgacgcct tcgagtccct tgccagtgct
     2281 ctctgccatc ttcggtcctc cagctcgttc agaggcgtct cgtggcgacc gccggcctac
     2341 acgctggtcc ctatcgtagg catcctgcca cgtgcccggc acgctcatcc tgttgttggg
     2401 cccggagtaa tctgaggcgc gcagcccgcc ttgacgtgcg tttgagagga gtgccccaaa
     2461 atggcgcatc gcgtgacgtt cattcctggc gacggggttg gcccggaact ctccgaggcc
     2521 actcgccggg tcctcgaagg gactggcgtc cagttcgact gggacgtcca ggaagccgg
//
LOCUS       DASWDC010000190         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1048223, whole genome shotgun sequence.
ACCESSION   DASWDC010000190 DASWDC010000000
VERSION     DASWDC010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048223"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VF995_06105"
     CDS             complement(<1..769)
                     /locus_tag="VF995_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX9343171.1"
                     /translation="MLARDRNQAERASTPLELLFDLCFVVAVAQAAARLHHALAEGGR
                     PVGGGVLSYALVFFAIWWGWMNFTWFASAYDTDDVPYRLLTLLQIAGVLVIAAGVPKA
                     FDSRDYAAVAAGYVLLRASMVSQWLRAALADPTGRRVALRFAAGIATVQVGWVVWLLT
                     VPQGWKLTGFAVLALLELAVPVFAERAGPPTAWHPEHIAERYGLFTLIVLGEVVAAAT
                     STVQSAITAGGVSATLLVAATGGLLLIFALWWSYFDHP"
     gene            917..1186
                     /locus_tag="VF995_06110"
     CDS             917..1186
                     /locus_tag="VF995_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HEX9343172.1"
                     /translation="MNIGASIFLLVVGAILTFAVNATTHPINVNVIGVILMVTGAGGM
                     LMSLLYWNSWSPWSRRRRVINPGPVLDGDRIIEERHYERNYPDYP"
     gene            complement(1442..2344)
                     /locus_tag="VF995_06115"
     CDS             complement(1442..2344)
                     /locus_tag="VF995_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9343173.1"
                     /translation="MSGSGQLSRRGTETEAGAGGPGSANGLRAGATAGTAERVEVTRL
                     RSYIVAEAAAVALGYVVTTLREGPPRSLSQWSLVLAGGAAVAVPLYVKSRREIRQDAA
                     ARAAEALAIETETKLRLILGDVMTPIVEVVGRVHLAASEADRNSLRGQLKQLVVEAVA
                     HLAGGERTRAIFFQLRGGVMRPDAWFGRSDPPNTVFTNRHDDRRGQEALLLVHFHDYV
                     MADDLDAEALPRGIERRPGAGYRSFISLAVFSDGQDLGMLTVDSPEPHAFDETDLNLM
                     RAMAQLLGAGLAERNGPLSRLLGS"
     gene            2599..3249
                     /locus_tag="VF995_06120"
     CDS             2599..3249
                     /locus_tag="VF995_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9343174.1"
                     /translation="MFAFARRRGDLPAAVSRLATLSIASAALGAVLVVLFGGAAQSAT
                     PPKSITVNGTAANKWDPSSTNVAVGGTVTFKVVAGGPHPVGAGPVSAAPNGDNSFVPG
                     KCGDFATMVNGGTCTITFKKAGTYQYFCKSHYSLGMVGTIVVGSGGTTTATTTGAATA
                     STAPVVSTPTQAAPPTPGKPGIYWAGYGLLALGALLALIAVVAYLRFAPGFRRQRR"
     gene            3336..>3947
                     /locus_tag="VF995_06125"
     CDS             3336..>3947
                     /locus_tag="VF995_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX9343175.1"
                     /translation="MVSTVSPTEVPELVDWDLAARVGRRVAGGDELDARPAWSRQFTE
                     LSTTADQAVADFTGLGGDLGPPVAEPLDRGQWVEANLATLRRVLRPLAERLATRKSWQ
                     ARGAVPNALRASTRTLTGAEAGTLLGYVGQRVLGQYDLPVPKPGGNGEGRVWYVVPNV
                     VSTERRHGFRPRDFRLWIALHETTHRRQFRGVAWLPGHVQGLLD"
BASE COUNT          598 a         1442 c         1360 g          547 t
ORIGIN      
        1 cgggatggtc gaagtaggac caccagagcg cgaagatcag caacagtccg ccggtggcgg
       61 ccaccagcag cgtcgccgac acgcccccgg cggtgatcgc ggactggacc gtgctggtcg
      121 cggcagccac gacctcgccg agcacgatca gggtgaagag gccgtagcgc tcggcgatgt
      181 gctcgggatg ccaggcggtg ggcgggccgg cgcgttcggc gaacaccggg accgccagtt
      241 ccagcagggc gaggacggcg aagccggtca gcttccaccc ctgcgggacc gtcagcagcc
      301 agaccaccca gccgacctgg acggtcgcga tccccgccgc gaagcgcagc gccacccgcc
      361 gcccggtggg atcggcgagg gcggcgcgca gccattgcga caccatcgag gcgcgcagca
      421 gcacgtagcc ggcggcgacg gcggcgtagt cgcggctgtc gaaggccttc ggcacccccg
      481 cggcgatcac cagcaccccg gcgatctgca gcagggtgag cagccggtag gggacgtcgt
      541 cggtgtcgta ggcggaggcg aaccaggtga agttcatcca cccccaccag atggcgaaga
      601 agaccagcgc gtagctgaga accccgccgc cgacgggccg cccgccttcg gcgagggcgt
      661 ggtggagccg cgcggcggcc tgcgcgaccg cgaccacgaa gcagaggtcg aacagcagct
      721 caaggggggt ggaggcccgc tctgcctggt tgcggtcgcg ggccagcatc gggcggcgcc
      781 agcgttgccg gctcgaaccc ttcgcccggc tcgtccgagg caggttcctg gcggttttcc
      841 aatgcaggca aacgggtatc acgtccccat gtaatgcccc ggtatcacca aggccgaggc
      901 cgagggagga ccagcaatga atatcggtgc gagcatcttc ctgttggtgg tgggggcgat
      961 cctgaccttc gcggtcaacg cgacgacgca tcccatcaac gtcaatgtca tcggcgtcat
     1021 cctcatggtc accggcgcgg gcggcatgct catgtcgctc ctgtactgga acagctggtc
     1081 accgtggtct cggcgccggc gcgtcatcaa tccaggcccc gtcctggacg gcgaccgcat
     1141 catcgaggaa cgccattacg agcgaaacta tccggactac ccgtaagcat cgagcgccgt
     1201 aggagcgagc gactcaggac tcaggtcgag gtgcggtccg cattcgtgag gccgttggca
     1261 ctggaaccgt gcagacggtc gcggaccgca tcgctcgctc tggcgagcgc tctgcggcgg
     1321 gcacgttcga agtagtcgtc cgacgccagc ctgcccgtgc tccaagcgcg tgcctcctgt
     1381 ggcactaccg ggccccgaac catcgcccac cctccgcgca ttctgcgcat cccgcgcatc
     1441 gtcagctccc cagcaacctt gacagaggac cgttcctctc ggcaaggccc gcaccgagca
     1501 actgcgccat cgcacgcatc agattgaggt cggtttcgtc gaaggcgtgc ggctccggcg
     1561 agtcgacggt gagcatgccg aggtcctggc cgtcgctgaa cacggccagg ctgatgaacg
     1621 agcggtagcc ggcgccgggg cgccgctcga tcccgcgtgg gagcgcctcg gcatccaggt
     1681 cgtcggccat cacgtagtca tggaagtgca ccagcagcag cgcctcctgg ccgcggcggt
     1741 cgtcgtgacg attggtgaac accgtgttgg gcgggtcgga gcggccgaac caggcgtccg
     1801 ggcgcatgac gccgccgcgc aactggaaga agatcgcccg ggtccgctcg ccgccggcga
     1861 gatgggcgac cgcctcgacc accagctgtt tgagctggcc gcgcagcgag ttgcggtcgg
     1921 cctcgctggc cgccaggtgg acacgcccga ccacctcgac gatcggggtc atcacgtcgc
     1981 ccaggatgag gcggagcttg gtttcggtct cgatcgcgag cgcctcggcc gcgcgggcgg
     2041 cggcgtcttg gcggatctcc cgccgcgact tgacatacag cggcactgcc accgccgcgc
     2101 cgccggccag caccaggctc cactgagaga ggctgcgggg cggcccttcc cgcagcgtgg
     2161 tgacgacgta gccgagcgcg accgccgccg cctcggcgac gatataggag cgcagccggg
     2221 tcacctcgac ccgctcggcc gtgccggcgg tggcgcccgc acgcagcccg ttggccgacc
     2281 cgggtccgcc cgcgccggcc tcggtctcgg tgccgcgccg tgacagctgc cctgaccctg
     2341 acatggtgat tggtacccgc tggtttatgg ctacatggca gtaactccgc gcaagtatcc
     2401 cagaatgctt ggttaacgcg ctgtcgccgg ggtgacggct gcgatagctg gaaatgacgc
     2461 gatgcgtcgt tatccgccac ttctagcggt tgcgggacgg cgtgcccggc gcgctacaat
     2521 ccgtcggcca gactgggcgc caagccgagg cacccagcgc cgcgccaagc agccacgcac
     2581 aggagcgcca tcagcctgat gttcgccttc gcccgtcgcc gtggggacct gcccgcggcg
     2641 gtctcccgcc tcgccaccct ctccatcgcc agcgccgcgc tcggcgccgt cctggtggtg
     2701 ctgttcggcg gtgccgccca gtcggccacc ccgcccaaga gcatcaccgt caacggcacc
     2761 gcggccaaca agtgggaccc ctcatcgacc aacgtcgcgg tcggcggcac cgtcaccttc
     2821 aaggtcgtgg ccggcggccc gcacccggtc ggcgcgggtc cggtcagcgc ggcacccaac
     2881 ggggacaaca gcttcgtccc gggcaaatgc ggcgacttcg ccaccatggt caatggcggc
     2941 acctgcacca tcaccttcaa gaaggccggg acctaccagt acttctgcaa gtcccactac
     3001 tccctcggca tggtcgggac gatcgtcgtc ggcagcggcg gcaccaccac cgccaccacc
     3061 accggggccg ccaccgccag caccgcgccg gtggtctcca ccccgaccca ggctgcaccc
     3121 cccacgcccg gcaaacccgg catctactgg gccggctacg gcctgctggc cctcggcgcc
     3181 ctgctggcgc tgatcgcggt ggtcgcctac ctgcgcttcg caccaggctt ccggcgccag
     3241 cgccgctgag ccggcccgct gccacgctcg gcgacccggc gcaggcaggg cgagtagcct
     3301 gaaagggcaa cgctgcctcg ccctttggag ctgccgtggt ttcgaccgtc tcgccaacgg
     3361 aggtccctga gctggtcgac tgggacctgg ccgctcgggt tggacgccgc gtcgccggcg
     3421 gcgacgagct ggatgcccgg cctgcctggt cgcgccagtt caccgagctg tccaccaccg
     3481 ccgaccaggc tgtcgccgac ttcaccggcc tcgggggcga cctgggcccg ccagtcgccg
     3541 agccgctgga ccgcggccag tgggtggagg ccaacctcgc cacgctgcgc cgggtgctgc
     3601 gcccgctggc cgagcgtctc gccacccgca agtcgtggca ggcccggggc gcggtgccga
     3661 acgcgctgcg cgcgagcacc cgcacgctga ccggcgccga ggcaggcacc ctgctcggct
     3721 atgtcggcca gcgcgtcctc ggccagtacg acctgccggt gccaaagccc ggcggcaacg
     3781 gcgagggccg ggtctggtac gtggtcccga acgtggtgag caccgagcgg cggcacgggt
     3841 tccggccgcg ggacttccgg ctgtggatcg cgctgcacga gacgacccac cggcgccagt
     3901 tccgtggggt cgcctggctg cccgggcacg tgcaggggct cctcgac
//
LOCUS       DASWDC010000191         5033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1298495, whole genome shotgun sequence.
ACCESSION   DASWDC010000191 DASWDC010000000
VERSION     DASWDC010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298495"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="VF995_06130"
     CDS             <1..126
                     /locus_tag="VF995_06130"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017570490.1"
                     /note="catalyzes the formation of
                     1,4-dihydroxy-2-naphthoate from O-succinylbenzoyl-CoA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX9343176.1"
                     /translation="GVQQLAGDATLLYYLSEEAQEGRDAYREKRRPDFAKFPRRP"
     gene            161..1060
                     /locus_tag="VF995_06135"
     CDS             161..1060
                     /locus_tag="VF995_06135"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX9343177.1"
                     /translation="MPSTPAPAPTPSLALWVRGARPRTLVAAVVPVLVGTAAASGAGW
                     IIAWRALAALVVSLALQVGVNYANDYSDGLRGTDTPERLGPVRLTASGLARPEQVRLA
                     AALAFSVAAACGLALAVAVDLRLLLVGVASIAAAVLYTGGPRPYGYAGFGELAVLLFF
                     GVVATTGSSYVQLGRVQPVALGASLAVGLAACAILLANNIRDVDGDRAAGKRTLAVRL
                     GRARARGLYVATLVAVFAVVALLGVRRPWVLLALAAVPFAVAPARMVLTREDGPGLVA
                     ALGGTARLQAVLGILLALGLWLS"
     gene            complement(1069..1839)
                     /locus_tag="VF995_06140"
     CDS             complement(1069..1839)
                     /locus_tag="VF995_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9343178.1"
                     /translation="MEPLRLHTVIDGAGAGPRVVLVHGFTQTGASMAPLAARLRDRGQ
                     GRFEVVRPDLPGHGGSSAVRAPMGEAARLLGDAGGAAAYLGYSLGGRVCLRLAVERPE
                     LVRALVLIGASPGLEGERERAERRAADERLAAELERDGVAPFLDRWLAGPLFATLPAA
                     AAGVEERLANSADGLAAALRLLGTGAQPPLWRRLGSLCMPVLLLAGALDGKFGALATR
                     MAVAIGPNARVALIAGAGHAAHLERPGEVAALVAEFLG"
     gene            1929..3638
                     /gene="menD"
                     /locus_tag="VF995_06145"
     CDS             1929..3638
                     /gene="menD"
                     /locus_tag="VF995_06145"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEX9343179.1"
                     /translation="MVDELVRCGLREAVLAPGSRSAPLAIALAEHPAVRLHVLLDERG
                     AAFFALGAAKRSGRPAAVLCTSGTAAANFHPAVVEAFHARAPLLLLTADRPPELRDTG
                     ANQTIEQRGLYGNALRWFHELGVPAGADLAQAGRWWRSATARAWAEALGPPAGPVQLN
                     LPLDEPLIPPKGALVGEPEPGRPDGAPWTATTETWRAPTAAEVEALAAAVRQAPRGLL
                     VAGWGARVRVEAADAFAAASGWPVLADPLSGLRRGPFAVAAYDALLRVPAFTATHRPE
                     LAVRVGAGPTSKVLTQWLGLGLGEGVPQVLLDPDGAWPDPLRAASTRLTADPNALLEA
                     AAEALMAGPERDGDAWRAAWMEADARALKAIGELLDGWDEPFEGRIARDLVAHLPHGA
                     TLVAGSSMPVRDLDAFALPRDGLSYVANRGASGIDGFAATALGTAAVAPGPVAALCGD
                     LTFLHDATSVLTARTRRPGVVFVVVDNDGGGIFSFLPQATLPPDLFEPLFGTPHGLDL
                     TAVATAAGLPSRRVERAADVSPALAEALTADTSTVLIVPSDRATNLSRHRAVAAAVAE
                     ALT"
     gene            complement(3908..4507)
                     /locus_tag="VF995_06150"
     CDS             complement(3908..4507)
                     /locus_tag="VF995_06150"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX9343180.1"
                     /translation="MEDPAEIVGRTLAANLRAMRQARGWRLEDLASRSGVSRGMLQQI
                     ETGRTNPSIATLARICATLGTSISRLVEPPEELGQVVRAADAEVYRAGRHGEGRLLIN
                     DGQAPFIELWDFLLADHDEMSAPAHPPGTRELLHLYQGRLEIEVGGAGFTLGAGDALR
                     MRGDRDHLYRNPGPGPARLTMAVVYSGDRDPRYAPSPIQ"
     gene            4588..>5033
                     /locus_tag="VF995_06155"
     CDS             4588..>5033
                     /locus_tag="VF995_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9343181.1"
                     /translation="MKTISHSIQINAPAGEVWEVLTDLAAYPDWNPFIREASGEVAVG
                     QRLTLKMFPADGKPMTFRPRVLVADPGMELRWIGRLVLPGIFDGEHSFTLTATATGGT
                     GLLQAEKFSGLLVPFTGKTIESTEKSFAALNQALKTRVESRSQAPHP"
BASE COUNT          599 a         1912 c         1843 g          679 t
ORIGIN      
        1 ggcgtccagc agctcgccgg cgacgcgacc ctcctctact acctgagcga ggaggcgcag
       61 gagggacgcg atgcctaccg ggagaagcgg cggccggact tcgccaagtt cccccgccgg
      121 ccctgacagc cctgacggcg acggtccccg gggtccatcg atgccatcca cgccagcgcc
      181 cgcgccgacg ccgagcttgg cgctgtgggt ccggggtgcg cgcccgcgca cgctggtcgc
      241 cgcggtcgtg ccggtgctgg tcgggaccgc cgcggccagc ggcgcggggt ggatcatcgc
      301 ctggcgggcg ctggcggcgc tggtcgtctc gctcgccctg caggtcggcg tcaactacgc
      361 caacgactat tcggacgggc tgcgcggcac cgacacgccc gaacggctcg ggccggtacg
      421 gctgaccgcc tcggggctgg cgaggccgga gcaggtgcgg ctcgccgccg cgctggcgtt
      481 ctcggtggcg gcggcatgcg ggctcgcgct ggcggtggcg gtcgacctgc ggctgctgct
      541 ggtcggggtc gcctcgatcg cggcggcggt gctctacacc ggcgggccgc gcccctacgg
      601 gtatgccggc ttcggcgagc ttgcggtgct gctgttcttc ggcgtggtcg ccaccaccgg
      661 ctcgtcgtac gtgcagctcg ggcgggtgca gccggtggcg ctgggggcgt cgctggcggt
      721 cggtctggcg gcctgcgcga tcctgctggc caacaacatc cgcgacgtcg acggcgaccg
      781 cgcggccggc aagcgcaccc tggcggtgcg gctcggccgc gcgcgcgccc gcggactgta
      841 cgtcgccacc ctggtggcgg tgttcgcggt ggtggcgctg ctgggggtgc gccggccgtg
      901 ggtgctgctg gcgctggccg cagtgccgtt cgccgtggct cccgcccgga tggtgctcac
      961 ccgcgaggac gggccgggcc tggtcgccgc cctcggcggc accgcccgcc tgcaggcggt
     1021 gctggggatc ctgctggcgc tgggcctgtg gctgtcgtga ccaggccgtc agcccaggaa
     1081 ttcggcgacc agcgcggcga cctcacccgg gcgttccagg tgcgcggcgt ggccggcgcc
     1141 ggcgatcagc gccacccgcg cgttggggcc gatcgccacg gccatgcggg tggcgagcgc
     1201 gccgaacttc ccgtccagcg cgccggcgag cagcagcacc ggcatgcaca gcgaaccgag
     1261 ccggcgccac agcggcggct gggcgccggt gccgagcagc cgcagcgccg cggccaggcc
     1321 gtcggcgctg ttggccaggc gctcctccac cccggccgcc gccgcgggca gggtggcgaa
     1381 cagcggcccg gccagccagc ggtccaggaa gggcgcgacg ccgtcgcgtt ccagctcggc
     1441 ggcgaggcgc tcgtcggcgg cgcggcgctc ggcccgctcc cgctccccct ccaggcccgg
     1501 ggaggcgccg atcagcacca gcgcccggac cagctccggc cgctcgaccg cgaggcgcag
     1561 gcagacccgc ccgccgaggg agtagccgag gtaggcggcg gctcccccgg cgtcgccgag
     1621 cagccgggcc gcctccccca tcggggcgcg gaccgcagag gagccgccgt ggccgggcag
     1681 gtcaggacgg accacctcga accgcccctg cccgcggtcg cgcagccgcg ccgcgagcgg
     1741 cgccatcgac gcgccggtct gggtgaagcc gtgtaccagc accaccctcg gaccggcacc
     1801 cgcaccgtca atcaccgtgt gcagccgaag cggctccacg cccggtcctc gctcctgtcc
     1861 cagatccatc tccgggagag tacgtgacca gcgccaccgc cgaccccacc cgccggttcg
     1921 ccctcaccat ggtcgacgag ctggtccgct gcgggctccg cgaggcggtg ctggcacccg
     1981 ggtcgcgctc ggcgccgctg gcgatcgcgc tcgccgagca tccggcggtg cggctgcacg
     2041 tgctgctgga cgagcgcggc gccgcgttct tcgcgctcgg cgccgccaag cggtcaggcc
     2101 ggccggcggc ggtgctgtgc acctcgggca cggccgcggc gaacttccat ccggcggtcg
     2161 tcgaggcgtt ccatgcgcgg gcgccgctgc tgctgctgac cgccgaccgg ccgcccgagc
     2221 tgcgcgacac cggcgccaac cagacgatcg agcagcgcgg cctctacggc aatgcgctgc
     2281 gctggttcca cgagctgggc gtgccggccg gcgcggacct ggcccaggct ggccgctggt
     2341 ggcggtcggc cacggcgcgc gcctgggcgg aggcgctcgg cccgcccgcc ggcccagtgc
     2401 agctcaacct cccgctcgac gagccgctga tccccccaaa gggtgcgctt gtcggcgagc
     2461 cggaacccgg ccgcccggat ggagcgccgt ggaccgccac gaccgagacc tggcgggcgc
     2521 ccaccgccgc ggaggtggag gccctggcgg cggcggtccg ccaggctcct cgcgggctgc
     2581 tggtcgcggg gtggggcgcc agggtccggg tggaggcggc cgatgcgttc gcggcggcaa
     2641 gcggctggcc ggtgctggcg gatccgctct cggggctgcg gcgcggcccc ttcgcggtcg
     2701 cggcctacga tgcgctcctg cgcgtcccgg cgttcaccgc cacccaccgg ccggagctgg
     2761 ccgtgcgggt gggggcaggc ccgaccagca aggtgctgac gcagtggctc gggctcggtc
     2821 tcggcgaggg cgtcccgcag gtcctgctcg acccggacgg cgcctggccc gacccgctcc
     2881 gggctgcttc gacccgcctc accgccgatc cgaatgccct gctggaggcc gccgccgagg
     2941 cgcttatggc cggacccgag cgggacgggg atgcctggcg ggccgcctgg atggaggccg
     3001 acgcccgcgc cctcaaggcg atcggcgagc tgctggacgg ctgggacgag ccgttcgaag
     3061 ggcggatcgc ccgcgatctg gtcgcccacc tgccccacgg ggcgaccctg gtcgccgggt
     3121 cgagcatgcc ggtccgcgac ctcgacgcct tcgcgctccc ccgcgacggg ctcagctatg
     3181 tcgccaaccg gggcgccagc ggcatcgacg ggttcgccgc caccgccctc ggcaccgccg
     3241 ccgtcgcccc gggcccggtt gcggcgctct gcggcgacct gaccttcctc cacgacgcca
     3301 ccagcgtgct gaccgcccgc acccggcgtc ccggcgtcgt cttcgtggtg gtcgacaacg
     3361 acggcggcgg catcttctcc ttcctgccgc aggcgaccct ccccccagac ctcttcgagc
     3421 ccctcttcgg caccccccac ggcctggacc tgaccgccgt ggcaaccgcc gccggcctcc
     3481 cctcccgccg ggtcgagcgc gccgccgacg tgtcccccgc cctcgccgag gcgctcaccg
     3541 cagacaccag caccgtcctg atcgtcccga gcgaccgcgc cactaacctc agccgccacc
     3601 gcgccgtcgc cgcggctgtc gccgaggcgc ttacctgact gaggtccctg cgtcggtgtc
     3661 agcgcaagcc cggtcaacac aaacggcgag gtggccgtga ccgcgctcag cgtggcgatg
     3721 ccggtggcgg cgaccaggca gccccgacga cgacctgggc gctggagaag cggcgatgca
     3781 gccggccggt gagctgggcc atcactaggc gtaattaggc gtgcggcgca acctgctcct
     3841 gggccggttc cttcgctgtt cggtgccttg ttggccggtc agtcggcgat gccgatgact
     3901 tgccctgtca ttggatcgga gatggtgcgt agcgggggtc gcggtcgccg gagtagacga
     3961 cggccatggt caggcgggcg ggaccggggc cggggttgcg gtagaggtgg tcgcggtctc
     4021 cgcgcatacg cagggcgtcg cccgccccga gggtgaagcc cgcgccgccg acttcgatct
     4081 cgaggcgtcc ctggtagagg tggagcagtt cccgcgtccc cggcggatgc gcgggagcgc
     4141 tcatctcgtc gtggtcggcg aggaggaagt cccacaattc gatgaagggg gcctggccgt
     4201 cgttgatgag cagccggccc tcgccgtggc gtccggcgcg gtagacctcg gcgtcggccg
     4261 cgcgcaccac ctggccgagt tcctcgggag gctcgacaag gcggctgatg gaggtgccga
     4321 gggtggcgca gatccgcgcg agcgtggcga tgctggggtt ggtacggccg gtctcgatct
     4381 gctgcaacat gccccggctg acccccgacc gtgaggccag gtcctccagc cgccagcccc
     4441 gcgcctgccg catcgcacgc aggttcgccg ccagcgtccg gccgacgatc tcagctggat
     4501 cctccattga cacctaccct cctgtggaac taatatatca gaactgaagc tagtatattg
     4561 gtcacacgtt cggctggagg cccaccgatg aagacaatct cccactcgat ccagatcaac
     4621 gccccggccg gcgaggtctg ggaggtgctc accgatctgg ccgcctaccc ggactggaac
     4681 cccttcatcc gcgaggcgtc cggcgaggtc gcggtcgggc agcggctcac tctgaagatg
     4741 ttccccgccg atgggaagcc gatgacgttc agacctcgcg tcctggtcgc cgacccgggc
     4801 atggaattgc gctggatcgg ccgcctcgtc ctgccaggca tcttcgacgg cgagcactcc
     4861 ttcacactca ccgccaccgc gaccggaggc accgggctcc tccaggccga gaagttctcc
     4921 ggcctgctcg tccccttcac cggcaagacc atcgagtcca ccgagaagag cttcgccgcc
     4981 ctcaaccagg cgctcaagac ccgtgtcgag agccgctcgc aagcccccca tcc
//
LOCUS       DASWDC010000192         3808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_547368, whole genome shotgun sequence.
ACCESSION   DASWDC010000192 DASWDC010000000
VERSION     DASWDC010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547368"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /gene="zwf"
                     /locus_tag="VF995_06160"
     CDS             complement(<1..1323)
                     /gene="zwf"
                     /locus_tag="VF995_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX9343182.1"
                     /translation="MSDNPLQVGERVRATPPPTAMVIFGGSGDLSHRKIIPALYNLEL
                     NRLLPQNFAILGVSRTAYSDEEYRADMRKAVEEFSRTQPIQPQIWDSFASRLYYAAGS
                     SDDPATYTRVGDLLERFDHEHGTDGNRLFYLSLIPKLFPDTIRGIGKAGIGGRGYDGG
                     SSRVVVEKPFGHDLASARELQALVTEVFPERDVFRIDHYLGKESVQNIIAFRFANGIF
                     EPVWNHRYVDHVQVTVAESLGIEHRGNFYETAGAIRDIIQNHALQVLALVGMEPPATF
                     EPEDVRNEKAKVLKALRRITGEAVDRHVVRGQYGHGLINGQEVVAYREEPDVDPDSVV
                     ETYVAAEFFVDNWRWAGTPFYLRTGKRLPKRATEVVVQFKAAPHLPFGEAARDGLEPN
                     ALVLRIQPNEGITMKFGAKVPGPKMAVRSVNMDFSYGTSFADDLPDAYE"
     gene            complement(1320..2228)
                     /gene="gnd"
                     /locus_tag="VF995_06165"
     CDS             complement(1320..2228)
                     /gene="gnd"
                     /locus_tag="VF995_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX9343183.1"
                     /translation="MDIGMVGLGRMGAGLTRRLLEDGHRVVAYDREAATTKAVAADGA
                     DPAASLEELVGKLSPPRTVWVMVPAGAPTETTITTLEPLLDPGDVLIDGGNSNYKDSV
                     RRAEHLASRSIKFLDAGTSGGIWGLREGFCLMVGGEPDAFAQAEPAFATLAPKGGYAH
                     VGPSGAGHYTKMVHNGIEYALLEGYAEGFEVLHASKYDFDLAQISELWRHGSVVRSWL
                     LDLLAGAYAHDHGPDKLAGYVEDSGEGRWTVQEALDLDVPAPAINLALLMRLRSREPE
                     SYAAKVIAALRAEFGGHPVRSPGASP"
     gene            complement(2274..3407)
                     /gene="tal"
                     /locus_tag="VF995_06170"
     CDS             complement(2274..3407)
                     /gene="tal"
                     /locus_tag="VF995_06170"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX9343184.1"
                     /translation="MTTTNPLEQLTQQGVAPWVDSIARDWLEKGELRRLRDQYYIVGT
                     TSNPTIFQKAMGEGSFYDDEIAQLAREGLDAQKIFEALALDDIRAAARDMAPVFQRTE
                     GLDGRISFEVPPDIADDAEATTAETARLWNLLDRPNVFIKIPATKAGLPAIRASIAAG
                     INVNVTLIFSIERYREVIDAFISGLEDRAKAGGDLSHLASVASFFVSRVDTNVDKKLA
                     AIIEQGGPEAGLARSRVGTAAIDNAKLAYEAFEESIATPRWRALAEAGARVQRPLWAS
                     TSTKNPAYRDVLYCEELVAHDTVNTMPIETIEAFADHGVVRGQTVKEDMGRAHTLWSD
                     LAELGIGEDTVGLELEREGVEKFAESYRGAVEAIERKRAKFAG"
     gene            complement(3476..>3808)
                     /locus_tag="VF995_06175"
     CDS             complement(3476..>3808)
                     /locus_tag="VF995_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010314629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9343185.1"
                     /translation="WVALAARDLLEAEGVATRVVSMPCWELFADQDQAYRDQVLPPGV
                     RARLAVEAASPFGWREWVGDGGDVLGVDHFGASAPGDQVLAAYGFTPDDVTARARALL
                     DRHRRVDQ"
BASE COUNT          511 a         1243 c         1419 g          635 t
ORIGIN      
        1 ctcgtaggcg tccggcaggt cgtcggcgaa cgaggtgccg taggagaagt ccatgttgac
       61 cgagcggacc gccatcttcg ggccgggcac cttggcgccg aacttcatgg tgatgccctc
      121 gttgggctgg atgcgcagca ccagggcgtt gggttccagg ccgtcgcggg ccgcctcgcc
      181 gaacggcagg tgcggcgcgg ccttgaactg gaccaccacc tcggtcgccc gtttcgggag
      241 ccgcttgccg gtgcgcaggt agaagggggt gccggcccag cgccagttgt ccacgaagaa
      301 ctcggcggcg acgtaggtct cgaccaccga gtcggggtcg acgtcgggct cctcgcggta
      361 ggcgacgacc tcctggccgt tgatcagccc gtggccgtac tggccgcgga ccacgtgccg
      421 gtcgaccgcc tcgccggtga tgcggcgcag cgccttcagc accttggcct tctcgttgcg
      481 gacgtcctcg ggttcgaagg tggctggcgg ctccatcccg accagcgcga ggacctgcag
      541 agcgtggttc tggatgatgt cgcggatcgc gccggcggtc tcgtagaagt tgccgcggtg
      601 ctcgatgccg agcgactcgg cgacggtgac ctggacgtgg tcgacgtagc ggtggttcca
      661 gacgggctcg aagatgccgt tggcgaagcg gaacgcgatg atgttctgga cgctctcctt
      721 gccgaggtag tggtcgatgc ggaacacgtc ccgctcgggg aacacctcgg tgaccagcgc
      781 ctgcagttcc cgggcggagg cgaggtcgtg gccgaagggc ttctccacca ccacccggga
      841 ggagcccccg tcgtagccgc ggccgccgat gccggccttg ccgatgccgc ggatggtgtc
      901 ggggaacagc ttcgggatca gcgacaggta gaagagccgg ttgccgtcgg tgccgtgctc
      961 atggtcgaaa cgctccagca ggtcgccgac gcgggtgtag gtggcggggt cgtcgctgga
     1021 gccggcggcg tagtacaggc ggcttgcgaa cgagtcccag atctgcggct ggatcggctg
     1081 agtacgggag aactcctcca ccgccttgcg catgtcggcg cggtactcct cgtcggagta
     1141 ggcggtgcgg ctgacgccga ggatggcgaa gttctgcggc agcagccggt tcagctccag
     1201 gttgtagagc gccgggatga tcttgcggtg ggagaggtcg ccggagccgc cgaagatgac
     1261 catggcggtc ggtggcggcg tggcacgcac ccgctcgccg acctggagcg ggttgtcgct
     1321 catgggctgg ctccggggga acggacgggg tggccgccga actcggcgcg cagggcggcg
     1381 atcaccttgg cggcgtagga ctccggttcg cgggagcgca gccgcatcag cagcgcgagg
     1441 ttgatcgccg gggcggggac gtccaggtcg agcgcctcct ggacggtcca gcggccctca
     1501 ccggagtcct cgacgtagcc ggcgagcttg tccggcccgt gatcgtgggc gtaggcgccg
     1561 gcgagcaggt cgagcagcca ggagcggacc acgctgccgt ggcgccacag ctccgagatc
     1621 tgggcgaggt cgaagtcgta cttggaggcg tgcagcacct cgaagccctc ggcatagccc
     1681 tccagcaggg cgtattcgat gccgttgtgg accatcttgg tgtagtggcc ggcgccggac
     1741 gggccgacgt gggcatagcc gcccttcggc gccagtgtgg cgaaggccgg ctcggcctgc
     1801 gcgaacgcgt cgggctcgcc gccgaccatc aggcagaacc cttcgcggag cccccagatc
     1861 ccgccggagg tgccggcgtc gaggaacttg atggagcggc tggccaggtg ctcggcgcgg
     1921 cgcaccgagt ccttgtagtt ggagttgccc ccgtcgatca gcacgtcgcc ggggtccagc
     1981 agcggctcca gcgtggtgat cgtggtctcg gtcggagcgc cggcgggcac catcacccac
     2041 accgtccgcg gtgggctgag cttgccgacc agctcctcga ggcttgcggc cgggtcggcc
     2101 ccgtcagcgg cgactgcctt ggtggtcgcc gcctcccggt cgtaggcgac cacccggtgg
     2161 ccgtcctcga gcagccggcg ggtgagcccg gcgcccatcc ggccgagccc caccatgccg
     2221 atgtccaccg tgcacctcct ggttcggagt ctcgtgagtc gtgcgtcttc tggttacccc
     2281 gcgaacttgg cgcgcttgcg ttcgatcgcc tcgaccgcgc cgcgatagct ctcggcgaac
     2341 ttctccacgc cctcgcgctc cagctccagg ccgacggtat cctcgccgat gccgagctcg
     2401 gccaggtcgc tccagagggt gtgggcgcgg cccatgtcct ccttgacggt ctggccgcga
     2461 accacgccgt ggtcagcgaa cgcctcgatc gtctcgatcg gcatggtgtt gacggtgtcg
     2521 tgggcgacca gttcctcaca gtagagcacg tcgcggtagg cggggttctt ggtcgaggtg
     2581 gacgcccaca ggggccgctg gacccgcgcg cccgcctcgg ccagtgcccg ccatcgcggc
     2641 gtggcgatcg actcctcgaa ggcctcgtag gcgagcttgg cgttgtcgat cgcggcggtg
     2701 ccgacgcggg agcgggccag gccggcctcg gggccgcctt gttcgatgat cgccgccagc
     2761 ttcttgtcga cgttggtgtc gacccggctg acgaagaagg aggcgacgct ggcgaggtgg
     2821 ctgaggtcgc cgccggcctt ggcgcggtcc tccagccccg agatgaaggc gtcgatcacc
     2881 tcgcggtagc gctcgatcga gaagatcagc gtcacgttga cgttgatgcc ggcagcgatc
     2941 gaggcgcgga tcgctggcag gcccgccttg gtcgcgggga tcttgatgaa gacgttgggc
     3001 cggtcgagca ggttccacag ccgggcggtc tcggcggtgg tggcctcggc gtcgtcggcg
     3061 atgtcgggtg ggacctcgaa cgagatgcgc ccgtccaggc cctcggtgcg ctggaatact
     3121 ggcgccatgt cgcgggcggc ggcgcggatg tcgtccaggg ccagcgcctc gaagatcttc
     3181 tgcgcgtcga ggccttcgcg ggcgagctgg gcgatctcgt cgtcgtagaa cgatccctcc
     3241 cccatcgcct tctggaagat ggtgggattg gaggtggtgc cgacgatgta gtactggtcg
     3301 cggagacggc gcagttcgcc cttctcgagc cagtcgcggg cgatcgagtc gacccagggc
     3361 gcgacgccct gctgggtgag ctgttcgagc gggttggtgg tggtcatggc actgctcctt
     3421 gcttgatcac gtcatctgct ccacgtcatc tgctgctgct ggtcttgccc cgcaatcact
     3481 ggtccactcg tcggtgtcgg tcgagcagcg ctcgggcgcg ggcggtcacg tcgtcgggcg
     3541 tgaagccata ggcggccagc acctggtcgc cgggggcgga ggcgccgaag tggtcgacgc
     3601 cgagcacgtc gccgccgtcg ccgacccatt cccgccagcc gaacgggctg gccgcctcga
     3661 ccgccaggcg ggcacgcacc cctgggggca ggacctggtc gcggtaggcc tggtcctggt
     3721 cggcgaacag ctcccagcag ggcatcgaca ccaccctggt ggccacgccc tcggcttcga
     3781 gcaggtcgcg ggcggcgagc gccaccca
//
LOCUS       DASWDC010000193        10585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1464444, whole genome shotgun sequence.
ACCESSION   DASWDC010000193 DASWDC010000000
VERSION     DASWDC010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10585
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464444"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..301
                     /locus_tag="VF995_06180"
     CDS             104..301
                     /locus_tag="VF995_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009592746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343186.1"
                     /translation="MPGTNTKKMTRAEAGRKGGQTTKKRYGDDFFGKIGRMGGKKGGE
                     TTKQRYGIEFYQRIGRKGGSR"
     gene            473..943
                     /locus_tag="VF995_06185"
     CDS             473..943
                     /locus_tag="VF995_06185"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX9343187.1"
                     /translation="MRSSIDVHNHLLADDVLHELPQLPGPLRDLAAAPAVLGLPPAAV
                     GRSTVFADQRGVVVAVAPADLEVDPAALREVTGRRELEPLAADQAPGLTGYLLPFVPP
                     VALECPAVVVVDEVVARQDVVYTAAGEPGVILKVRGVDLIKATSAVVTRVTRDD"
     gene            977..1426
                     /locus_tag="VF995_06190"
     CDS             977..1426
                     /locus_tag="VF995_06190"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9343188.1"
                     /translation="MRTARATSAGGVIIAEPVPRSRIALVARRSPRGSLQWTLPKGTQ
                     EEGETLTDTALREAREESGLEVALIGELATIDYWFVWAPEQTRYHKFVHYFLMHAVGG
                     DLSLHDDEMEEAAWFTLEEARDLMAFANERRLLDRIPEVLDDWSETG"
     gene            1423..3591
                     /locus_tag="VF995_06195"
     CDS             1423..3591
                     /locus_tag="VF995_06195"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEX9343189.1"
                     /translation="MNSGGGRRVRTGVSLLASLLTLVVAAGVGTLPASARTRQAQQGE
                     ALTLTLAKISPAAGPGSKAPLVFQLVVRDNGAGPLQGLRVDTLAGGPLRTHSEFQQVA
                     GETAPSLAGFSLLDRWQVPGQPRVAPGAALTLPAHALSLPPRGDPAAVLPLVLRVSGD
                     SASGPVSAQLLTFAVDLNGQVAAAQRLRIALLAPLHEPTQRTPAGAFIDQGRGLEAQL
                     APSAPLGAIAAALSRPGAPKVTLVVDALLTEEASLLVGNPGSKIASQFTDNLRAAADQ
                     NPQNPPSAFPYDTADLPSLLRGGYDTEALASVLRGQNLLREFGTTPSTALAWPVSGPI
                     DTATLKGIALSGATTVVLPARLLPTSAALTQNATVNLGPGVAPLRRALVPDTTLSTPL
                     SSAQMTTEPVAWAQRILAESAVTWLEQPNSGQPRGVLLAPPQSWRPSPAFFRALAQGL
                     AAAGWLRLEPAAQLATDVPQGPDRQPRQLVPYGAADAHLGLPASYLASIAKTRASLAS
                     FNRAVGDNFALSDDFDRDLLIAASSDWRPSPARPRGASFINAVKKGMRTVYSKVGVDR
                     TPRTLTSRTGQLPITVINDGDQPLEVMLQLSSPRVDLPAATQPFLLAAHRRVTKRIEV
                     STRTTGSFPILVEVLTPDGRQITQASVTLVSTAFDRVALLLAGGAAAFLLVWWGRKRA
                     RRRGGDAGAGAAPATPSSPGASTSASTGAPVDGVVKAGTP"
     gene            3642..5339
                     /gene="murJ"
                     /locus_tag="VF995_06200"
     CDS             3642..5339
                     /gene="murJ"
                     /locus_tag="VF995_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX9343190.1"
                     /translation="MSVGEANPVPAAGSPASASPAGRAGRQPAEPSLARSTAVMAAGT
                     LLSRVTGLLRVIVLVSVLGVTEGKLADVYNVANTTPNIVYELVLGGILSSIFVPVFVE
                     VRRTRGMRTANYVACAVMTVAIVGLGLLALVTMLTAPWIIKVYIHSGTPAQRAEAQRL
                     GGQMLVMFMPQIVFYGVGAVMTGLLNANRRFGVPMFAPILNNLTVIAVALAFYFIVGD
                     QVPPLGQVTTGEKLLLGLGTTAGVVAMTMVQWPFLRRAGFRFHLEWHLRDPAIKKMAT
                     LSAYTVGYVVVNQLGYAVIPILAYGVQGGYSAYTYAFTFFQLPHGVFAVSVMTALLPP
                     MSEQAVARDWDAFRASLAKGLRLTAVVLLPASVGYLALAGPIVRLLLAHGVIGGSSVV
                     LLTRVLTMFVLGLLPFSAFQLCLRAFYALQNTRTTFLVNIAAVAVNVVLDLALFHALP
                     TPWKVPGLALGSSVAYTVGVILLLRRLSTKIGGLGSRRILAAVERMLVASLGMGAVAW
                     LAAAGAAHLLGRGTFASLLTVVVGVGAGLATYLGMARLLGVAEVGAVLALVRRRSAA"
     gene            complement(6084..6308)
                     /locus_tag="VF995_06205"
     CDS             complement(6084..6308)
                     /locus_tag="VF995_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343191.1"
                     /translation="MAVPPAPLPARGSAQEGLAPAREALALAARARAARAAASWRARA
                     WRARAWRASASSRRRWSSRARLTADLSWAR"
     gene            6323..6703
                     /locus_tag="VF995_06210"
     CDS             6323..6703
                     /locus_tag="VF995_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX9343192.1"
                     /translation="MPGRTCPRGRSPSPSPQLGKPYVWGATGPSSYDCSGLTSTAYHS
                     AGLALPRTAAEQWWAGRHVNRSGLRPGDLVFYATNTSDPATIHHVGIYVGNGEMIEAP
                     HTGADVRVASIARRDYIGATRPAG"
     gene            6919..8448
                     /locus_tag="VF995_06215"
     CDS             6919..8448
                     /locus_tag="VF995_06215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9343193.1"
                     /translation="MLAERYELSEQLASGRLTSVWRGHDRVLGRDVIVKVLHPYLAAD
                     PAFRARFHQEAVNAARLTHPNIVALYDTGEHEHDQEGVVYLVMELVRGATLRQVLKRY
                     GALPPAKAAQLACEIIAALEYAHQSGVVHRNLKPTNVVLAEDDSVKVGDFSITAAASD
                     DDPERTGELLGLDAAGYVAPEILAGDEPDGRADVYGLGACLYEMLTGRAPYASRSLTV
                     HGTGQLFSPRAVRADVPRELDAVVQKAMALDPDRRFQSAREMAAALARSAADADGAPG
                     ALVPVEHNLPPATDTEFIPAEPEGFLRHEGRWLGWTLALVGMAAVLVVVGLTLSKSVN
                     LHLPGVPGNKGGGGSTTTTGQHATTLKFTQAIPFDPPPGDGSEHAEKAANATDGDPAT
                     AWITQEYNNPVFGGIKTGVGLILKVDHAVRPGELDLTLAKPGASFTVYGATGDGGSFP
                     TSLSDGWTRLGSSTGTRQQTNLQLSGSGSYQYYLVWFTSLPAAGDGRFQAGIAEASLR
                     A"
     gene            8474..9181
                     /locus_tag="VF995_06220"
     CDS             8474..9181
                     /locus_tag="VF995_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9343194.1"
                     /translation="MSDPSDEALLAAHLRGDRQAFGVLVRRHERRVYGLCLRLLGNRE
                     DAEDATQEAFLAALRHAASFRRTAAFSTWLYRIAVNAATDQGRRRARARSVPLLEEGP
                     KQLAPVVPDATEEVAGAIAVQAALARVPLDFRVALVLCDLYQLPSVQASEILGIPVGT
                     VKSRAFRGRLALAELLSGAGIGASASGATSATSAEGLPERAKARAPVAGGGPAGTGTA
                     FPTSESAEPERRQPDQE"
     gene            9186..10136
                     /locus_tag="VF995_06225"
     CDS             9186..10136
                     /locus_tag="VF995_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX9343195.1"
                     /translation="MRHQPDHPDQQQLAAFQAGELGRRERARLETHLGACAGCAALLA
                     GAEQAGRLLAALPLEPELPPGLQERLATAVEREIATQPSRPSRAAQQSRRSRQAPAWY
                     RRRGALGLLGAAAALLLLVALVPRLHPVASPKRTSSGAASSAAGPATAAGQAAAGEAA
                     QPPTRVLAVINAPHGFSARDLKADLAADPSARAAFSAARSGVPGPTPLNPKSSAQPDA
                     GTATTTASSSGAAPTGPSGNKAMTSIQASCLAAAARLAPGARPALVVTTVYQGKPATV
                     LVTVAAGNGGGTVAATLWYFAGGSCGQQPLHHEETTVTLP"
     gene            complement(10158..>10585)
                     /locus_tag="VF995_06230"
     CDS             complement(10158..>10585)
                     /locus_tag="VF995_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343196.1"
                     /translation="TCSSCGDPICARCMVNTVVGQKCPRCARQSSRARGTPGVPLMAR
                     AFGAGLAVAFAGGFVLVKVGMAGLLLAVLYGFAVGEVVRRAAKRRVHTGFGAAAAAAL
                     LLGMSAVALVFSVSPLAPQLLLFDVIGAVVAYLRASGTW"
BASE COUNT         1471 a         3999 c         3734 g         1381 t
ORIGIN      
        1 ggagtgagct gcgtcgatcg tccgtccgag gcctctccaa acctttttct gcggtttagg
       61 agaaactcag gcagggcaac cacatcgatc aggaggtggt ccaatgccag gcacgaacac
      121 gaagaagatg accagggccg aggctggccg gaagggtggc cagacgacga agaagcgcta
      181 cggcgacgac ttcttcggca agatcggccg aatgggtggc aagaaaggcg gcgagaccac
      241 caagcagcgc tacggtatcg agttctacca gcgcattggc cggaagggag ggtcgcgctg
      301 aggatccacc gacccgtcgc cagcttcgga gcccccggcc accgggggct cctctatact
      361 caggtcccga tccggcatcg ccgtagcggt ccgccgtctc ggcgacgccg ttcggggttc
      421 ccgtcgtgac ctgatcctgg tgccccggcg ccggcaccag ctgggggaag tagtgcgttc
      481 cagcatcgac gttcacaacc acctgctcgc ggacgacgtg ctccatgagc tgccgcagct
      541 tcctggcccg ctgcgcgacc tggccgccgc gcccgcggtg ctcggccttc cgcccgcggc
      601 cgtcggccgg tcgaccgtct tcgccgacca gcggggagtc gtggtggccg tcgccccggc
      661 cgacctggag gtcgaccccg ccgcgcttcg cgaggtgacc ggccgccgcg agctggagcc
      721 gctggccgcc gaccaggctc cagggctgac cggctacctg ctgcccttcg tcccgccggt
      781 ggcgctggag tgccccgccg tggtcgtcgt ggacgaggtc gtcgcgcggc aggacgtggt
      841 ctacaccgcc gccggcgagc ccggcgtgat cctcaaggtc cggggcgtgg acctgatcaa
      901 ggcgaccagc gcggtggtga cgcgcgtgac ccgcgatgac tgacgggcgg gccaccggga
      961 ccgggagctg ataggcgtgc gcaccgcccg tgccacctcg gctggagggg tgatcatcgc
     1021 ggagcctgtg ccccggtcac ggatcgcgct ggtggcacgc cgctcgcccc gggggagcct
     1081 gcagtggacc ctgccaaagg gcacccagga ggagggcgag acgcttaccg atacggcgct
     1141 gcgcgaggcc cgcgaggaga gcgggctgga ggtggcgctg atcggcgagc tggccaccat
     1201 cgactactgg ttcgtgtggg ctcctgaaca gactcgttac cacaagttcg tacactactt
     1261 tctcatgcac gccgtgggcg gcgacctgtc cctccacgac gacgagatgg aggaggccgc
     1321 ctggttcacg ctggaggagg cacgcgacct gatggccttc gcgaacgagc gccgcctcct
     1381 cgaccgcatc ccagaggtac tggacgactg gagcgagacc gggtgaacag tggcggcggg
     1441 cggcgcgtgc ggaccggggt gtcgctgctc gcgtcgctgc tcacactggt ggtcgcggcc
     1501 ggcgtcggga cgctgccggc ctcggcgcgc acccggcagg cgcagcaggg cgaagcgctc
     1561 accctcaccc tggcgaagat ctcgccggcc gccgggcccg gatccaaggc cccgctcgtc
     1621 ttccagctgg tggtgcgcga caacggcgcc ggaccgctcc agggcctgcg ggtggacacg
     1681 ctggccggcg ggccgctgcg gacccactcc gagttccagc aggtcgccgg cgagaccgcg
     1741 ccgagcctgg ccgggttcag cctgctcgac cgctggcagg tgccaggcca gccgcgggtc
     1801 gcccccggcg cggccctgac gctgccggct cacgccctgt ccctgccgcc gcgcggcgac
     1861 cctgcggcgg tgctgccgct ggtgctgcgg gtcagcggcg acagcgccag cgggccggtc
     1921 agtgcgcagc tgctgacctt cgcggtggac ctcaacgggc aggtggcggc ggcgcagcgc
     1981 ctgcggatcg cgctgctggc gccgctccac gagccgaccc agcgcacccc cgccggcgcc
     2041 ttcatcgacc agggccgggg cctggaggca cagctggctc cgagtgcccc cctgggcgcg
     2101 atcgccgccg cgctgagccg tccgggagct ccgaaggtta ccctggtggt cgacgcgctg
     2161 ctcaccgagg aggccagcct gctggtgggc aaccccggct ccaagatcgc gagccagttc
     2221 accgacaacc tccgcgccgc ggccgaccag aacccgcaga accccccgag cgccttcccc
     2281 tacgacaccg ccgacctgcc ctcgctgctg cggggcggct acgacaccga ggcgctcgcc
     2341 tcggtgctgc gcggccagaa ccttctcagg gagttcggca ccacgccctc caccgcactg
     2401 gcctggccgg tgagcggacc gatcgacacc gccaccctca aggggatcgc cctcagcggc
     2461 gcgaccacgg tggtgctgcc agcgcggctg ctgccgactt cggcggcgct cacccagaac
     2521 gccaccgtga acctcgggcc cggggtggcg ccgctgcggc gggcgctggt ccccgacacg
     2581 accctctcaa ccccgctcag cagcgcccag atgaccaccg agccggtcgc ctgggcccag
     2641 cggatcctgg ccgagagcgc ggtgacctgg ctggagcagc cgaacagcgg ccagccgcgc
     2701 ggcgtgctgc tggcgccgcc gcagtcgtgg cggccgtcgc ccgcgttctt ccgggcgctg
     2761 gcgcaagggc tggccgccgc gggctggctg cggctggagc cggccgccca gctcgccacc
     2821 gacgtcccgc agggccccga caggcagcca cgccagctgg tcccctacgg cgccgcggac
     2881 gcgcacctcg ggctgcccgc cagctacctg gccagcatcg ccaagacccg cgccagcctc
     2941 gccagcttca accgcgcggt gggggacaac ttcgcgctct ccgacgactt cgaccgcgac
     3001 ctgctgatcg ccgcgtcgag cgactggcgg cccagccccg cgcgcccgcg cggggccagc
     3061 ttcatcaacg cggtcaagaa ggggatgcgg acggtctaca gcaaggtcgg ggtggaccgc
     3121 accccgcgga cgctcacctc gcgcaccggg cagctgccga tcaccgtgat caacgacggc
     3181 gaccagccgc tcgaggtgat gctgcagctc tcctcgcccc gggtcgacct gccggcggcg
     3241 acgcagccgt tcctgctggc cgcgcaccgc cgcgtcacca agcggatcga ggtgagcacc
     3301 cggaccaccg gcagcttccc gatcctggtc gaggtgctca cccctgacgg ccggcagatc
     3361 acccaggcgt cggtcaccct ggtctcgacc gccttcgacc gggtcgcgct gctgctcgcc
     3421 ggcggcgccg ccgccttcct gctggtctgg tggggccgca agcgggcacg gcggcggggc
     3481 ggcgacgccg gcgcgggggc tgccccggcc acgccctcca gccccggcgc cagcaccagc
     3541 gccagcaccg gcgcccccgt ggacggcgtg gtgaaggcgg ggacgccatg aggcaccagg
     3601 gcgagcgcgg tgccgcggcg aacgacgatg gcggtgcgtg aatgagcgtc ggcgaggcga
     3661 acccggtgcc ggcggcaggc tccccggcct cggcctcgcc ggcgggccgg gcgggacgcc
     3721 agccggccga gccgagcctg gcccgttcga ccgcggtgat ggcggccggg acgctgctgt
     3781 cccgggtcac cggcctgctg cgggtgatcg tgctggtgtc ggtcctcggc gtcaccgagg
     3841 gcaagctcgc cgacgtctac aacgtcgcca acaccacccc gaacatcgtc tacgagctgg
     3901 tgctcggcgg gatcctgtcg tcgatcttcg tgccggtgtt cgtcgaggtg cgccgcaccc
     3961 gcgggatgcg gacggccaac tacgtggcct gcgcggtgat gaccgtcgcg atcgtggggc
     4021 tcggcctgct ggcgctggtc acgatgctga ccgcgccctg gatcatcaag gtctacatcc
     4081 acagcggcac gcccgcgcag cgggccgagg cccagcggct cggcggccag atgctggtca
     4141 tgttcatgcc gcagatcgtg ttctacgggg ttggggcggt gatgaccggg ctgctcaacg
     4201 ccaaccggcg cttcggcgtg ccgatgttcg ccccgatcct gaacaacctg acggtgatcg
     4261 cggtggcgct ggccttctac ttcatcgtcg gcgaccaggt gccgccgctc ggccaggtca
     4321 ccaccggcga gaagctgctg ctcggccttg gcaccaccgc tggggtggtg gcgatgacga
     4381 tggtgcagtg gccgttcctg cgccgcgccg ggttccgctt ccatctggag tggcacctcc
     4441 gcgacccggc catcaagaag atggccaccc tgtcggcgta cacggtcggc tacgtggtgg
     4501 tcaaccagct cggctacgcg gtgatcccga tcctcgccta cggcgtccag gggggctaca
     4561 gcgcctacac ctacgccttc accttcttcc agctgcccca cggggtgttc gcggtgtcgg
     4621 tgatgacggc gttgctgcca cccatgagcg agcaggcggt ggcgcgcgac tgggacgcct
     4681 tccgggcatc cctggccaag gggctgcgcc tgacggcggt ggtgctgctg cccgcctccg
     4741 tcggctacct agcgctcgcc gggccgatcg tgcggctgct gctggcgcac ggcgtcatcg
     4801 gcgggagcag cgtggtgctg ctgacccggg tgctgaccat gttcgtgctc ggcctgctgc
     4861 cgttctcggc gttccagctg tgcctgcggg cgttctacgc gctgcagaac acccgcacca
     4921 ccttcctggt gaacatcgcc gcggtggccg tgaacgtggt gctcgacctg gcgctgttcc
     4981 acgccctgcc gacgccgtgg aaggtccccg ggctggcgct cggctccagc gtcgcctaca
     5041 cggtcggggt gatcctgctg ctgcggcgcc tttccaccaa gatcggcggg ttggggagcc
     5101 ggcgcatcct cgcggcggtc gagcggatgc tggtcgccag cctgggcatg ggcgcggtcg
     5161 cctggctcgc cgccgccggt gccgcccacc tgctcggccg ggggacgttc gcctcgttgc
     5221 tcacggtggt ggtcggggtg ggcgcgggcc tcgccaccta cctcggcatg gcgcggctcc
     5281 tcggggtggc ggaggtcggc gcggtgctgg cgctggtccg gcgccgctcc gccgcgtagg
     5341 cgggccggaa gatatcgggc agcgcgggac accaccgatc aagtcgatcg tgccgtacgt
     5401 acctatgtcc tcgaactcct tgaccattac ctttccggtg tggcacaacg gacgacggtc
     5461 gcagtcccga caacccaatg gagggtcctt cccctgcgtc ccccgcgtcc ggtccggacc
     5521 gttcttccga atctgctggt cattgccggt ctcctggccg gcctcctcgg caaccccgct
     5581 gcggccggcg cctccgagag ccccgcccac aaggcggctc ggctgcgcgc cgaggccgtc
     5641 caggtgcagg ccaccatcga ccaaatgaac gaccaggtcg aggtcctggt cgagcgctac
     5701 gacgccaacc aggaagcgct cggccgcacc gccgccgcgc aggccggcac caggcggcgg
     5761 atcgacgccg ccaccgccgc gctcaacgcc gggcaggacc agctcgacca gcgtgcctgg
     5821 gacgcctaca ccaccgggcc ggtgaccccg ctggcggcgc tgctgggggc ggccaccatc
     5881 caggacatgc tgaccagcgc gaagtaccag gaacgggtca tcgacgctga caacgctgcc
     5941 gtggtcagga tccgccaggc ccggcacgcc ctggggaccc tcgccgcgca gcttgccgcc
     6001 cagcgccggg ccgaggagca gctccgggcc gccctgagaa gccagcgccg gcggatcgag
     6061 gctggcctgg cacgccagca ggcctacctg gcccagctca agtcggctgt caagcgggcc
     6121 ctggacgacc agcgccgccg tgaggaggcg ctggcccgcc aagccctcgc ccgccaggcc
     6181 ctcgcccgcc agctggccgc cgcccgcgcc gcccgggcgc gggcggccag tgccagtgct
     6241 tcccgcgccg gcgccaggcc ctcctgggcg ctgccgcggg ccggcagcgg cgccggcggg
     6301 accgccacgc tcgccgacgg cggtgccggg gcggacgtgt cctcgcgggc ggtcgccttc
     6361 gccttcgccc cagctcggca agccctacgt ctggggcgcg accgggccgt ccagctacga
     6421 ctgctcaggc ctgacgtcga ccgcctatca cagcgccggc ctcgccctgc cgcgcaccgc
     6481 cgccgagcag tggtgggccg gccgtcacgt caaccggtcc ggcctgcggc ctggcgacct
     6541 ggtcttctac gccaccaaca ccagcgatcc ggccaccatc caccacgtcg gcatctacgt
     6601 cggcaacggc gagatgatcg aggcccccca caccggcgcc gatgtccggg tcgcctcgat
     6661 cgcacgccgc gactacatcg gcgcgacccg cccagccggg taggaatcga cccatcgggg
     6721 ccccgcggcc accacggcac gggcctgcgg ttcctacggc cgcgggacca tgccccccga
     6781 accccccggc gcgtagcacc gtccggcgaa gtagccgcct gctagactgc ctgagtcgag
     6841 atccccaggg aaaagaagcg actgaagccg gttgctttcc atatgagcat gtcgcaggtt
     6901 ggttcagggc taccccgagt gctcgctgag cggtacgagc tcagcgagca attggcttcg
     6961 gggcggctga cgagtgtttg gcgtggccac gaccgcgtgc tcggccgcga cgtcatcgtc
     7021 aaggtgctgc acccgtacct ggccgccgac ccggccttcc gcgcccgctt ccaccaggag
     7081 gcggtgaacg ccgcacgcct cactcatccc aacatcgtgg cgctgtacga caccggcgag
     7141 cacgagcatg atcaggaggg cgtcgtctac ctcgtcatgg agttggtccg cggcgccacc
     7201 ctgcggcagg tgctcaagag gtacggcgcc ctgccgcccg ccaaggcggc ccaactcgcc
     7261 tgcgagatca tcgccgcgct ggagtacgcg caccagagcg gcgtggtaca ccgcaatctc
     7321 aagcccacca acgtggtgct ggccgaggac gacagcgtca aggtggggga cttctccatc
     7381 acggcggcgg ccagcgacga cgacccggag cggaccggcg agctgctggg gctcgacgcc
     7441 gccggctacg tcgcgcccga gatcctcgcc ggggacgagc ccgacggccg cgccgacgtc
     7501 tacggcctgg gcgcctgcct gtacgagatg ctgaccggcc gggcgccgta tgcctcgcgt
     7561 tcgctgacgg tgcacggcac cgggcagctg ttctcacccc gggcggtacg cgcggacgtg
     7621 ccccgtgagc tggacgcggt ggtgcagaag gcgatggcgc tcgaccctga ccgacgcttc
     7681 cagagcgccc gggagatggc ggcggccctg gcccgctcgg ccgccgacgc cgacggcgcc
     7741 ccgggcgccc tggtgccggt cgagcacaac ctgccgcccg ccaccgacac cgagttcatc
     7801 ccggcggagc cggagggatt cctgcgccac gagggccgct ggctcggctg gaccctcgcc
     7861 ctggtcggca tggccgccgt cctggtggtg gtcgggctga cgctcagcaa gagcgtcaac
     7921 ctccacctcc ccggcgtgcc cggcaacaag ggcggcggcg gctcgacgac caccaccggg
     7981 cagcacgcca ccaccctcaa gttcacccag gcgatcccgt tcgacccgcc gcccggagac
     8041 ggctcggagc acgccgagaa ggccgcgaac gcgactgacg gcgacccggc cacggcgtgg
     8101 atcacgcagg agtacaacaa cccggtcttc ggcggcatca agacgggcgt cggcctgatc
     8161 ctgaaggtcg accatgcggt ccggcccggc gagctggacc tgaccctggc gaagcccggg
     8221 gcgagcttca cggtctacgg cgcgaccggg gacggcggct ccttcccgac cagcctgtcg
     8281 gacggctgga cgcggctcgg cagctccacc gggaccaggc agcagaccaa cctacagctc
     8341 agcgggagcg gcagctacca gtattacctg gtctggttca ccagcctgcc agcagcgggg
     8401 gacggcagat tccaggccgg catcgcggag gcgtccttgc gagcgtagag ggtgaggcag
     8461 gaggcgtgcc ggtttgagcg atcccagcga cgaagcgctc ctggccgccc acctgcgtgg
     8521 cgaccggcag gccttcggcg tgctggtgcg acgccacgag cgccgcgtct acggcctctg
     8581 cctgcgcctc ctcggcaacc gcgaggacgc cgaggacgcc acccaggagg ctttcctggc
     8641 ggccctgcgc cacgccgcca gcttccgccg cacggcggcc ttctcgacct ggctgtaccg
     8701 catcgcggtg aacgccgcca ccgaccaggg gcggcggcgt gccagggccc gcagcgtccc
     8761 gttgttggag gagggtccca agcagctcgc accggtcgtc ccggacgcca ccgaggaggt
     8821 ggccggcgcg atcgcggtgc aggccgccct cgcccgggtc ccgctcgact tccgggtcgc
     8881 gctggtgctc tgcgacctct accagctccc gagcgtgcag gcgtccgaga tcctgggaat
     8941 cccggtcggg acggtgaaga gccgcgcctt ccgtggccgc ctcgccctgg ccgagctgct
     9001 ctccggcgct ggcatcggcg ccagcgccag cggcgccacc agcgccacca gcgccgaggg
     9061 cctgccggag cgcgccaagg cccgcgcgcc ggtcgccgga ggagggcccg cgggaaccgg
     9121 aaccgccttc ccaacgtccg aatctgcaga accagaacgt cgccagcccg accaggagta
     9181 gcagcgtgcg ccaccagccc gaccaccctg accagcagca gctcgccgcc ttccaagcgg
     9241 gggagctggg tcgtcgtgag cgcgcacgcc tcgaaacgca cctgggagcc tgcgccggct
     9301 gcgcggccct gctggccggc gccgagcagg ccggccgcct gctggccgcc ctgccgctgg
     9361 agcccgagct gccccccggc ctccaggagc gtctggcgac cgcagtcgag cgcgagatcg
     9421 cgacccagcc ctcccggcct tcccgggccg cccagcaatc acggcgatca cggcaggcgc
     9481 cggcctggta tcggcgccgg ggggccctcg gcctgctcgg cgccgccgcc gccctgctcc
     9541 tgttggtggc cctggtgccg cgactccacc ccgtcgcgtc gccgaagcgt acgagcagcg
     9601 gcgccgccag cagtgcggcg ggcccggcca cagccgcggg gcaagctgcc gctggagagg
     9661 cggcgcagcc cccgacgcgg gtcctggcgg tcatcaacgc cccgcatggc ttctcagcgc
     9721 gggacctgaa ggccgacctg gccgccgacc cgagcgcacg cgcggcgttc agcgccgccc
     9781 ggagcggcgt tccgggccca accccgctga acccgaagag ctcggcgcag ccggacgctg
     9841 gcaccgcgac caccacggcg tcgtcgtcag gtgcggcccc cacggggccc tccgggaaca
     9901 aggcaatgac cagcatccag gccagctgcc tcgctgccgc cgccagactg gcgccgggag
     9961 cccgcccggc cctcgtggtg accaccgtct accagggaaa acccgcgacc gtgctggtca
    10021 ccgtcgcagc tgggaacggc ggcggcaccg tggcggccac gctctggtac ttcgcgggtg
    10081 gcagctgcgg ccagcagcca ctccaccatg aggagaccac cgtcacgctg ccgtagggcg
    10141 ctggtgcgcg cgaggcgcta ccaggtcccg ctggcgcgca ggtaggcgac cacggcgccg
    10201 atcacgtcga acagcagcag ctggggcgcg agcggggaga ccgagaacac cagggcgacc
    10261 gcgctcatgc cgagcagcaa cgcggcggcg gcggccgccc cgaagccggt gtgaacgcgc
    10321 cgcttggcgg cacggcggac cacctcgccg acggcgaacc cgtacaggac cgccagcagc
    10381 aatccggcca tacctacctt gaccagcacg aacccgccgg cgaaggcgac cgccaggccc
    10441 gccccgaacg ccctggccat cagcggcact ccgggggtgc cgcgggcacg cgacgactgc
    10501 cgcgcgcagc gcgggcactt ctgcccgacc accgtgttga ccatgcaccg ggcgcagatc
    10561 gggtcgccgc agctcgagca ggtga
//
LOCUS       DASWDC010000194         7521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1049890, whole genome shotgun sequence.
ACCESSION   DASWDC010000194 DASWDC010000000
VERSION     DASWDC010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049890"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VF995_06235"
     CDS             <1..949
                     /locus_tag="VF995_06235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9343197.1"
                     /translation="TLERGATYGLGRRELAERLPVGQGIAGAVAARGTPMVLAIPPKG
                     AAPRTGEPKATTQVSVPLLAQGRVLGVLSLYDRQGQGQGQGQGVGQEGDFTLEDAEAL
                     AAFAVQAAVAVENVQLHAEAERLSVTDAMTGAWNYRYFERRLEQEIERSRRFGRVFSL
                     LLLDIDHFKWVNDRHGHQRGDEVLVELARRVTASVRDIDTFARYGGEEFVLILPETDV
                     EGGLAAAEKLRKVVGGQPFAARPDDPGISLTISVGVAGYPQHAAGPNVLLRAADSAMY
                     EAKVQGRDRVVSARPGLLAIDGGSRTLPGGSAGGSTKAE"
     gene            1042..1929
                     /gene="galU"
                     /locus_tag="VF995_06240"
     CDS             1042..1929
                     /gene="galU"
                     /locus_tag="VF995_06240"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX9343198.1"
                     /translation="MIPAAGLGTRFLPASKAQPKEMLPVVDTPAIQYVVEEAVRAGLD
                     DILIVIGRGKYAIEDHFDRSWELEKLLADKGSDQRLRQVQEIADMAEIHYIRQKMPLG
                     LGHAVSVAEQHVGNEPFAVLLGDDLIGEDEKLLANMVDCYERQQTSVVAAMEVPRSQI
                     SAYGAVAPGNEVEPEVFRVTDIIEKPEPEEAPSNLAVIGRYVLHPDIFDALRATKPGA
                     KGEIQLTDAIKLLTQAQGGKGALAMAFTGTRYDVGDKNDYLRATVELAARRDDIGPAF
                     VSFLRDFVSQLDAPDGTRR"
     gene            1972..3279
                     /gene="glp"
                     /locus_tag="VF995_06245"
     CDS             1972..3279
                     /gene="glp"
                     /locus_tag="VF995_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX9343199.1"
                     /translation="MADHFPPEQGRVDPPDMVPFEQARAEVLARLPPLPAREVGLGDA
                     LGCVLAADIHAGEHLPPFANSAMDGFAVRSADVQAASAQRPVALRLAGEVFAGSARLP
                     RVPPGGAVRIMTGGSLPPGADAVVPVEQTKLAAGGDPGSGEVLVHVAVGQRAFVREAG
                     EDVREGTLVLERGRVLDPAAIGMLASVGRRTVPVVPRPRVAVVSTGDELVDPGDPLGP
                     GQIRDSNSWLLVAQARRAGAEAFRCGRLHDDPGALRKGFALAAAEGDLVLTSGGVSVG
                     DRDYTRQVLAELGDVRGMRVAMQPGMPQAFGFAAGTPLYGLPGNPVSCFVVFELLVRP
                     ALRRLAGHPDDRLDHVRVPAVLGEPVRSPAGKVSFLRVRLEQEAGEGSPRAFLTGPQG
                     SGVLSSCVAADGLAVIPAADRELAAGAVVEVVLLRDDPPSLVP"
     gene            3276..3839
                     /gene="moaC"
                     /locus_tag="VF995_06250"
     CDS             3276..3839
                     /gene="moaC"
                     /locus_tag="VF995_06250"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX9343200.1"
                     /translation="MTEPRSQPEPRLTHVDERGAARMVDVSAKPESERVAVAGCRVLL
                     APATLELLASGGLAKGDALAVARIAGIMGAKRTPELIPLCHPLPITGVEVALDVEADA
                     VAITATVRTTGRTGVEMEALTAAATAALALYDMVKGVQRDARVDGLRLLAKRGGRSGD
                     YRAPDPDPDPDPDPDPDPDIGAGGDNG"
     gene            3832..4359
                     /locus_tag="VF995_06255"
     CDS             3832..4359
                     /locus_tag="VF995_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX9343201.1"
                     /translation="MAEGLRGLRVALLTVSDRVAEGSRDDQGGDVLCALLEAAGATLA
                     GRAVVPDERPRIVAALRSLAAGAEVVLTTGGTGLGPRDVTPEATREVLDREAPGIPEA
                     LRHASLAITPFAMLSRAAAGLAGRTLIVNLPGNPKAVAEEWEVLAPVLAHAVATATGP
                     VPDASHLHHRHRQGH"
     gene            4402..4893
                     /locus_tag="VF995_06260"
     CDS             4402..4893
                     /locus_tag="VF995_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343202.1"
                     /translation="MLRCRSLVLVGDFLAGQLRPPAADERCIVSFAGPDPSGTDRRRQ
                     LVARRRRVLVVLALAALVGLRAGVALGGRWWLAEAATGSLLAGYLLALALAGARAQRR
                     HQRGRQPLPEAPGVAITAKEAAEPTPTIAASVPAEALPPPGPAAERRSRRPLSRRLRP
                     APK"
     gene            4890..5564
                     /locus_tag="VF995_06265"
     CDS             4890..5564
                     /locus_tag="VF995_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEX9343203.1"
                     /translation="MSLEEIERGLRDRFGAKYRAREVGIGNARQAIRRAANAIRAVHR
                     GEFEVADRLLVEARELLDIAGSVLAAHPDVYYAGFLQDAQKEYAEARATRALVAGEPP
                     PPPEDLGVGDAPYLNGLAEAIGELRRHVLDLLRAGADDRAARGFQAMEDIHALLANID
                     YPDAITGGLRRSTDVARAVIERTRSDLTLTGIQRDLADALRHAADPGADPDPGADPDP
                     GSHGSR"
     gene            5575..5976
                     /locus_tag="VF995_06270"
     CDS             5575..5976
                     /locus_tag="VF995_06270"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018830298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9343204.1"
                     /translation="MDSEDLPLGGFNKLLGLRLEEASADLVVATVPVTPDLHQPYGLV
                     HGGVYSSVVEATASVGAALWLGDRGRTVGIGNQTEFLRAVRDGVLRIVATPIKRGRST
                     QLWQVDVTDDHGHLAAQGRVRLMNLPGGSFE"
     gene            complement(5980..7443)
                     /locus_tag="VF995_06275"
     CDS             complement(5980..7443)
                     /locus_tag="VF995_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9343205.1"
                     /translation="MPLELLRPHDAFFLHTETARVQQHVGGLAILDPSGRPSGPLRLD
                     EVAAHLAGRLDQVPRLRQRLAFPPLGLVRPAWVDDDAFDLGAHLRTLHLAGSGGRADL
                     CAAVEAVMSQPLDRSRPLWEAYLIEGLPDGLQGFLVKLHHAIADGLAALELARHILDL
                     TPDPTPSPGPPGPVDTASSPPPDVDSLIPGGRPQGGSRLFFAALRAHLAGPLRSVAQG
                     SAHLVTRPRWSLRRAFDTLLGVWELARRGRAPRSPITGPIGPRRRIAFTEVPLPWINR
                     IRRELGCGTSDIALTATAEALHRLLAARPASGAPGGGGAPATLRTMLPIAVRPWSKQA
                     APGTWTAAFSIDLPVGPMAPAERLAAVRALTRAARHTHQARAAKFLMDSVGTWAPAWV
                     HARAARFAYRGRWFNLIVTALRGVPVPVYLLGARVAAAYPIMPLAEDMGFAVAALSWA
                     DQLTIGLTADWDTIPELDAVAKAITDCVEQLYKAAKR"
BASE COUNT          970 a         2744 c         2877 g          930 t
ORIGIN      
        1 caccctggag cgtggtgcca cctatggcct cggccggcgg gagctggccg agcggctgcc
       61 ggtcgggcag ggcatcgccg gcgcggtggc ggcgcggggc acgccgatgg tgctggcgat
      121 cccgcccaag ggcgccgccc cccgcaccgg cgagcccaag gcgaccaccc aggtgagcgt
      181 gccgctgctg gcgcagggac gggtgctcgg ggtgctcagc ctctacgacc gccagggcca
      241 gggccagggc cagggccagg gcgtcgggca ggagggcgac ttcaccctgg aggacgccga
      301 ggcgctggcc gccttcgcgg tgcaggccgc ggtggcggtc gagaacgtcc agctccacgc
      361 cgaggccgag cgcctgtcgg tgaccgacgc gatgaccggg gcgtggaact accgctactt
      421 cgagcggcgc ctggagcagg agatcgagcg ctcccgccgc ttcgggcggg tgttcagcct
      481 gctgctgctg gacatcgacc acttcaagtg ggtcaacgac cgccacggcc accagcgggg
      541 cgacgaggtg ctggtcgagc tggcccgccg cgtcaccgcc agcgtccgcg acatcgacac
      601 cttcgcccgc tacggcggcg aggagttcgt actgatcctg cccgagaccg acgtcgaggg
      661 cggtctggcc gccgccgaga agctccgcaa ggtcgtcggc ggccagccgt tcgccgcccg
      721 gcccgacgac cccggcatct cgctgaccat ctcggtcggg gtcgccggct acccgcagca
      781 cgccgccggg cccaacgtgc tgctgcgcgc cgccgacagc gccatgtacg aggccaaggt
      841 ccagggccgc gaccgggtgg tctccgcccg acccggcctg ctggcgatcg atggtggttc
      901 gcggaccctt ccagggggca gtgctggggg aagcacgaag gctgagtagg gccagccgcc
      961 agccgctatg atcggccttt ccagtttcaa gaccgcagca gcagctttcc gggggtgggg
     1021 gacgtgaagg tacgcaaagc agtgattccc gcggctgggc tcggcacccg cttcctgccg
     1081 gcctccaagg cgcagccaaa agagatgctg ccggtggtcg acaccccggc gatccagtac
     1141 gtggtggagg aggcggtccg cgccggtctc gacgacatcc tgatcgtgat cgggcggggc
     1201 aagtacgcca tcgaggacca cttcgaccgc tcctgggagc tggagaagct cctcgccgac
     1261 aagggctccg accagcggct gcggcaggtc caggagatcg ccgacatggc ggagatccac
     1321 tacatccgcc agaagatgcc gctcggcctc ggccacgcgg tcagcgtcgc cgagcagcac
     1381 gtcggcaacg agccgttcgc ggtcctcctc ggcgacgacc tcatcggcga ggacgagaag
     1441 ctgctcgcga acatggtgga ctgctacgag cggcagcaga ccagcgtggt cgcggcgatg
     1501 gaggtgccgc gctcccagat cagcgcctac ggcgcggtcg cgcccggcaa cgaggtcgag
     1561 ccggaggtct tccgggtcac cgacatcatc gagaagccgg agcccgagga ggcaccctcc
     1621 aacctcgcgg tgatcggccg ctacgtgctc cacccggaca tcttcgacgc cctgcgcgcc
     1681 accaagccgg gcgccaaggg cgagatccag ctcaccgacg cgatcaagct gctcacccag
     1741 gcgcagggcg gcaagggcgc gctggcgatg gccttcaccg gcacccgcta cgacgtcggc
     1801 gacaagaacg actacctgcg cgccaccgtc gagctggccg cccgccgcga cgacatcggc
     1861 ccggcgttcg tctccttcct gcgcgacttc gtctcccagc tcgacgcgcc cgacggcacc
     1921 cggcgctgac gcgccggcgc cgcaccggac ccgtcccgta cgctgtcgcc gatggccgac
     1981 cacttcccac cagagcaggg ccgggtggac cccccggaca tggtgccgtt cgagcaggcc
     2041 cgcgccgagg tgctggcgcg gctgccgccg ctgccggccc gcgaggtggg gctcggcgac
     2101 gcgctcggct gcgtgctcgc cgccgacatc cacgccggcg agcacctccc gccgttcgcg
     2161 aactcggcca tggacgggtt cgcggtgcgc agcgccgacg tccaggcggc aagcgcgcag
     2221 cgcccggtgg cgctccgcct ggccggcgag gtgttcgccg gcagtgcccg cctgccgcgg
     2281 gttccgcccg gcggcgcggt gcggatcatg accggcgggt cgttgccgcc gggtgccgac
     2341 gcggtggtgc cggtcgagca gaccaagctc gccgccgggg gcgaccccgg aagcggcgag
     2401 gtcctggtcc acgtcgccgt ggggcagcgc gccttcgtgc gggaggctgg cgaggacgtg
     2461 cgcgagggca cgctggtgct ggagcgcggg cgggtgctcg acccggccgc gatcggcatg
     2521 ctcgcctcgg tcgggcggcg caccgtcccg gtcgtgcccc ggccgcgggt cgcggtggtc
     2581 tccaccggcg acgagctggt cgatcccggc gacccgctcg gccccgggca gatccgcgac
     2641 tccaactcct ggctgctggt cgcccaggcc cggcgagcgg gcgcggaggc gttccgctgc
     2701 ggccgcctgc acgacgaccc cggcgcgctg cgcaaggggt tcgcgctggc ggcggccgag
     2761 ggcgacctgg tgctcacctc cggcggcgtg tcggtggggg accgcgacta caccaggcag
     2821 gtgctcgccg agctgggcga cgtgcgcggc atgcgggtgg cgatgcaacc gggcatgccg
     2881 caggcgttcg gcttcgccgc cgggacgccc ctgtatgggc tgcccggcaa cccggtctcg
     2941 tgcttcgtgg tcttcgagct gctggtccgc ccggcgctgc ggcgcctggc cggccacccc
     3001 gacgaccgcc tcgaccatgt ccgggtgccc gccgtgctgg gcgagccggt ccgcagtccc
     3061 gccgggaagg tgtccttcct gcgtgtccgt ctggagcagg aggccggcga ggggagccca
     3121 cgtgccttcc tcaccgggcc gcagggctcc ggagtgctca gctcctgcgt ggccgcggac
     3181 ggcctggcgg tgatcccggc agcggaccgg gagctggccg ccggcgcggt cgtcgaggtc
     3241 gtgctgctgc gcgacgaccc tccctccctc gttccatgac tgagccgcga tcccagcccg
     3301 agcctcggct gacccacgtg gacgagcgtg gcgccgcgcg gatggtcgac gtctccgcca
     3361 agccggagag cgagcgcgtg gcggtggcgg gctgccgggt gctgctcgcg cccgcgaccc
     3421 tggagctgct cgccagcggc gggctggcca agggcgacgc gctggcggtc gcccggatcg
     3481 ccgggatcat gggggcgaag cggactcccg agctgatccc gctgtgccat ccgctgccga
     3541 tcaccggcgt ggaggtcgcc ctcgacgtgg aggccgacgc ggtcgcgatc accgccaccg
     3601 tccgtacgac cggccggacc ggcgtggaga tggaggcgct cacggcggcg gccaccgccg
     3661 cgctcgccct ctacgacatg gtcaagggcg tccagcgcga cgcccgcgtc gacgggctgc
     3721 gcctgctcgc caaacgcggc ggccgctccg gggactaccg tgccccggac ccggacccgg
     3781 acccggaccc ggacccggac ccggacccgg acatcggcgc cgggggcgac aatggctgag
     3841 gggttgaggg ggctccgggt cgcgctgctc actgtgagcg accgggtcgc cgaggggagt
     3901 cgtgacgacc agggcggcga cgtcctctgc gcgctgctgg aggcggccgg cgcgaccttg
     3961 gccggccggg cggtggtgcc cgacgagcgg ccgcggatcg tcgccgcgct ccggtcgctc
     4021 gccgccggcg ccgaggtggt gctgaccacg ggcggcaccg gcctcggccc ccgtgacgtc
     4081 acccccgagg cgacccgcga ggtgctggac cgcgaggcac caggcatccc ggaggcgctg
     4141 cggcacgcga gcctggcgat cacgcccttc gcgatgctct cgcgtgccgc cgccggtctc
     4201 gccgggcgca ccctgatcgt gaacctcccc ggcaacccca aggccgtcgc cgaggaatgg
     4261 gaggtcctcg cgccggtcct ggcccacgcg gtcgccaccg cgacgggccc ggtccccgac
     4321 gcgagccacc tgcaccaccg tcaccgccag ggtcactaac cggtaacggg cgcggaagcc
     4381 cgttgctcca ccttggggtg cgtgttacgg tgccgctcgc ttgtcctggt cggggacttc
     4441 ctcgccggcc agctccgtcc ccccgccgcc gacgagaggt gcatcgtgag cttcgcgggt
     4501 cccgatccgt ccgggactga ccggcgccgt cagctggtgg cccgacgccg gcgcgtgctc
     4561 gtcgtcctcg cgctcgcggc gctggtcggg ctgcgtgccg gcgtcgcgct cggcggccgc
     4621 tggtggctgg cggaggcggc gacggggtcg ctgctcgccg gctacctgct cgccctggcg
     4681 ctggcgggcg ctcgcgcgca gcgccggcac cagcgcgggc ggcagcctct ccccgaagcg
     4741 cccggagtgg cgatcacggc gaaggaggcc gccgagccca cgccgaccat cgcggcgagc
     4801 gtcccggccg aggccctgcc gcctcccgga ccggcggcgg agcgaaggtc gcggcggccc
     4861 ttgtcccggc gcctgcggcc cgcgccgaaa tgagcctgga ggagatcgaa cgcggcctgc
     4921 gcgaccgctt cggcgccaaa taccgtgccc gcgaggttgg catcggcaac gcccggcagg
     4981 cgatccgccg cgcggccaac gccatccgcg cggtccaccg gggcgagttc gaggtcgccg
     5041 accgcctgct ggtcgaggcg cgggagctgc tcgacatcgc cgggtcggtg ctggccgccc
     5101 accccgacgt ctactacgcc gggttcctgc aggacgccca gaaggagtac gcggaggcgc
     5161 gggccacccg cgcgctggtc gccggcgagc cgccgcctcc tcccgaggat ctcggcgtcg
     5221 gcgacgcccc ctacctgaac ggcctggccg aggcgatcgg cgagctgcgc cgccacgtcc
     5281 tggacctgct ccgcgccggc gcggacgacc gcgctgcccg cggcttccag gcgatggagg
     5341 acatccacgc gctgctggcc aacatcgact accccgacgc gatcaccggc ggcctgcgcc
     5401 gctccaccga cgtcgcccgg gcggtgatcg agcgcacccg cagcgacctc accctcaccg
     5461 gcatccagcg tgacctcgcg gacgcgctgc gccacgcggc cgaccccggc gccgaccccg
     5521 acccgggcgc cgaccccgac cccggttccc acggcagccg gtaggctgtg ctccatggat
     5581 agcgaggacc tcccgctcgg cgggttcaac aagctgctcg gcctccggct ggaggaggcg
     5641 agcgctgacc tggtggtggc gaccgttccg gtcacgccgg acctgcacca gccctacggg
     5701 ctcgttcacg gcggggtcta ctcgtcggtg gtggaggcga ccgccagcgt cggcgcggcg
     5761 ctgtggctcg gcgaccgggg gcggaccgtc gggatcggca accagactga gttcctgcgg
     5821 gcggtccgcg acggcgtgct gagaatcgtc gcgaccccga tcaagcgcgg tcgctcgacc
     5881 cagctgtggc aggtcgacgt cactgacgac catgggcatc tggcggcgca ggggcgggtc
     5941 cggctgatga acctgccagg cggctcgttc gagtaggcgc taccgtttgg cggccttgta
     6001 gagctgctcg acgcagtcgg tgatggcttt ggcgacggcg tccagctccg ggatggtgtc
     6061 ccagtcggcg gtgaggccga tggtgagctg atcggcccag ctgagggcgg cgaccgcgaa
     6121 gcccatgtcc tcggccagcg gcatgatcgg atatgcggcg gccacccggg cgcccagcag
     6181 gtagaccggg accggcacgc cccgcagggc ggtgacgatc aggttgaacc agcggccccg
     6241 gtaggcgaaa cgcgccgcgc gggcgtggac ccatgccggc gcccaggtgc cgaccgagtc
     6301 catcaggaac ttggcggccc gggcctggtg ggtgtggcgc gccgcccggg tcagggcgcg
     6361 cacggcggcg agccgctcgg ccggcgccat cgggccgacc gggaggtcga tggagaaggc
     6421 ggcggtccag gtcccgggcg ctgcctgctt gctccagggc cgcaccgcga tcggcagcat
     6481 ggtccgcagg gtggccggcg ctccacctcc ccccggggca ccggaggccg ggcgggcggc
     6541 gagcagccgg tggagcgcct cggcggtggc ggtgagggcg atgtcgctgg tgccgcagcc
     6601 aagttcgcgc cggatccggt tgatccaggg cagcgggacc tcggtgaagg cgatacggcg
     6661 ccgcgggccg atcggcccgg tgatcgggct gcgtggagcg cgaccgcggc gggccagctc
     6721 ccagacgccg agcagggtgt cgaacgcgcg ccgcagcgac cagcgaggcc gggtcaccag
     6781 atgggcgctg ccctgggcga ccgaccgcag gggcccggcg agatgggccc ggagcgccgc
     6841 gaaaaacaac cgcgacccac cctgggggcg cccccccggg atcagactat caacgtcggg
     6901 aggggggctg ctcgcggtat ccacaggccc cggcggtccc ggggaggggg tgggatccgg
     6961 ggtcaggtcg aggatgtggc gggccagttc gagggcggcg aggccgtcgg cgatggcgtg
     7021 gtgcagcttg accaggaagc cctggaggcc gtcggggagg ccctcgatca ggtaagcctc
     7081 ccagagaggg cgggagcggt cgagcggctg ggacatgacc gcctccacgg cggcgcacag
     7141 gtccgcgcgg cccccggagc cagccaggtg gagggtgcgc aggtgggcgc ccaggtcgaa
     7201 ggcgtcgtcg tcgacccagg cggggcggac cagcccgagg ggcgggaacg ccagccgctg
     7261 gcgcaggcgc gggacctggt cgaggcggcc ggcgaggtgg gcggcgacct cgtccagacg
     7321 cagcggaccg gagggtcgcc cggacgggtc caggatcgcc aggccgccga cgtgctgctg
     7381 cacccgcgct gtctcagtgt ggaggaagaa agcgtcgtgg ggacgcagca gctccaaggg
     7441 caaggaccgc tccggcgttg ccataggggc ggccggtgct actggtggcg atcgacggcg
     7501 ccgccctcgc cgtcgccgcg g
//
LOCUS       DASWDC010000195        16330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_442218, whole genome shotgun sequence.
ACCESSION   DASWDC010000195 DASWDC010000000
VERSION     DASWDC010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16330
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442218"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VF995_06280"
     CDS             <1..375
                     /locus_tag="VF995_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9343206.1"
                     /translation="QVLTNLLDNAVKYSPDGAPIRIEARRDAGQAVLAVEDAGPGVPA
                     ADRERIFERFARLEQGEAHRAGGVGLGLWIARQLARNLGGDLLLAPPSRPDGGARFEL
                     RLPLVQNVLGTERSMTLAATPR"
     gene            710..1195
                     /gene="greA"
                     /locus_tag="VF995_06285"
     CDS             710..1195
                     /gene="greA"
                     /locus_tag="VF995_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX9343207.1"
                     /translation="MSQSSDERVTYLTPEAYERLSTELDELKTEGRERVSQQIGVARE
                     HGDIRENADYDAAKNEQGMMEARIRQLEKLLRSAVVGIPPATGKAGPGMVVKLNIAGD
                     EEMYFLGSREEAIDGMDVLSLRSALGRAVVGRTPGDEFSYTTPTGRELPVKLLEAQPR
                     A"
     gene            complement(1202..2188)
                     /locus_tag="VF995_06290"
     CDS             complement(1202..2188)
                     /locus_tag="VF995_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9343208.1"
                     /translation="MPHAHKDPTAPPALTVEGAADRLAPRYRVAAVEARTGIPASTLR
                     SWERRYGFPRPLRTATGQRLYSDHDVALIRFLARQRAEGLSIGQVTALLRQAGTEADP
                     EQLLERLIRALGAFDEAGAERCFAAAVRLLGLEGAALALVELAIGAIAEGAGDVEGAG
                     RVAAEHFASEVLRRELLRLLDAFPPGRGQPLLVACGPEEQHELGALLLRLLLRARGHH
                     VVYLGPRLPGPVLARAAAALDPAGLAVSLTMGESLERTIGWLRRDRPSALLALALPGA
                     GAVHGSSRLWWGGQALLEAAEAAGHLPGEVVTGSAADGADTIDRGLRRRGPR"
     gene            complement(2127..3143)
                     /locus_tag="VF995_06295"
     CDS             complement(2127..3143)
                     /locus_tag="VF995_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005886282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX9343209.1"
                     /translation="MRLAAFDVGTNSTRLLVADVDDGRISEQFDREMIITRLGKGVDA
                     NRRFDPGALRRTLEVLGEYAATCDRFGVGPQRRRLVATSATRDVANREEFLDGVRRLV
                     GVEAEVLTGEQEAAASYLGATGDLDGDARTLVVDIGGGSTEFIVGNRDPEAAISLEIG
                     CVRLFERHLHSDPPTADQVAALRADVREHLERVQTVFDPSSATRVVGVAGTVTTVAAL
                     TLDLDHYDPERIHHAVLEAPRIAATARRLCRLTIAQRAALPVMAPGREDVIAAGALVL
                     DEICRTFRFARVVASETDILDGVLLELARNRSPARDRSPGGPGAARAQGSDGSARTDR
                     GGRG"
     gene            complement(3140..3682)
                     /locus_tag="VF995_06300"
     CDS             complement(3140..3682)
                     /locus_tag="VF995_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEX9343210.1"
                     /translation="MPPTGGADPRGEREWAAGLPDATAAERRVIEAQIGRRVRGAIAV
                     AARCRYGLPAVIRTAPYLDDGTPFPTLYWLACPAARVAVSRLEAAGWNAALSERVAAD
                     PALAEAHAAAHESYLAQRDALGRLPGDPGVGGLPGRVKCLHVLYAHEAATGADPVGRM
                     IRPVIDPIDCPGPCVEDLDG"
     gene            complement(3770..4138)
                     /locus_tag="VF995_06305"
     CDS             complement(3770..4138)
                     /locus_tag="VF995_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX9343211.1"
                     /translation="MSAEIGAVVPGTVVRIAPYGAFIKLESGETGLVHISEIDRNYVR
                     SVEEHLRLDDQVMVKVLGVKEDGKIDLSIKQAAPDWQPEAPRRRAAKDPEFEQKLKKF
                     MRSSEERLVDVKRQREGRRT"
     gene            complement(4356..4754)
                     /locus_tag="VF995_06310"
     CDS             complement(4356..4754)
                     /locus_tag="VF995_06310"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX9343212.1"
                     /translation="MRAALAAGVGGRRVGLTPRGVVLVLVVFLLAVTAVYPLREHVSQ
                     QARIRQLQAKQAALDAANAQLAAERARLKDPAYLEQLAKQNLNVVRPGEQQWEITGTP
                     PAAKPPPAPKPPPKPPWYTRFWRWLTGTSH"
     gene            complement(4751..6052)
                     /gene="eno"
                     /locus_tag="VF995_06315"
     CDS             complement(4751..6052)
                     /gene="eno"
                     /locus_tag="VF995_06315"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009408921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9343213.1"
                     /translation="MASIELVLAREVLDSRGNPTLECEVQLDNGGLGRAIVPSGASTG
                     RFEAVELRDGETRYGGKGVLGAVRHVNEVIAPSLLDEDGEDQRAIDHLLLDLDGTDNK
                     SKLGANATLGVSMAVARAAADSLGIPLFRHLGGMAAHLLPVPMMNVLNGGVHADNEVD
                     FQEFMIVPVGAPSFREALRMGAEVYHTLKSVLKGKGLSTGVGDEGGFAPNLPGNEAAL
                     QLLVEAIGQAGYEPGPEVALALDPASSEFYRDGRYQLTAEGRSLDSAGMVELYAELCD
                     RYPIVSIEDGLAEDDWDGWAALTEALGDRVQLVGDDIFVTNVTRIRRGLAQGVGNSVL
                     VKVNQIGTLTETFDAVELAHRGGWTTVVSHRSGATEDTTIADLAVALGCGQIKSGAPA
                     RSERVAKYNQLLRIEETLGAAARYPGRTAFPRSFPPGGETP"
     gene            complement(6102..7199)
                     /gene="mazG"
                     /locus_tag="VF995_06320"
     CDS             complement(6102..7199)
                     /gene="mazG"
                     /locus_tag="VF995_06320"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX9343214.1"
                     /translation="MTRVVLVAASPRLPLLWAPQTWRALDAAKPVYLLDDQHPSLPAL
                     EVGEIPWEVLAPAGDTGPAGRDLLLVGQGLDVEGVALARRQADALLALAKERGSATLL
                     LPPEHDGPLVQLVCDRAARQHVEVEAVYPLGEPKGSALLDLVATETRLRGPGGCPWDR
                     EQTHASLARHLVEEAYELVEAIDDADADPEHLREELGDLLLQVVFHAQIAEDAGSFDI
                     DGVARAITEKLHRRHPHVFGDLQVATSDEVVSNWERIKREEEGRGDPFAGIPKALPAL
                     QFAAKLRRKAAGHGPDIDQARRDVRAALGGLEGPAVAGIAEGRQQRIGELLFSVVGLA
                     LALDVEPEAALRTVSRQYRERFRERLREHDS"
     gene            complement(7216..8268)
                     /locus_tag="VF995_06325"
     CDS             complement(7216..8268)
                     /locus_tag="VF995_06325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9343215.1"
                     /translation="MRRRSLTGVPVALCLAALFALVASGCGKSSSSQAAVVNGTIIPV
                     SQLKEEVSAKLGAASQSTASTAQDMVTLTQQSLEGLIQFQIVLDGAKQKGVAVTDGDI
                     DGRVNTIKAQAQQQGTTYEALLAQNNLTDALLRQQLRVQLAVNSVATKLVPYQADDVL
                     QRIMNQHKSDYLEVHVRHVLVKDQKTANTVHDQLIKGGDWSAVAKKYSIDPGSKDKGG
                     DLGFAAKGTFVAEFEKAATSLANQGNCKGKTSGSCASPISAPVKSQYGYHVIQVTGVR
                     LPQLTDDLRQKLDPNITTRRQTAVDSWFKGLLKDAAVTVNPRFGKWDQASGHVVDRST
                     APGAYTNSSSSVPVTS"
     gene            complement(8340..11882)
                     /gene="mfd"
                     /locus_tag="VF995_06330"
     CDS             complement(8340..11882)
                     /gene="mfd"
                     /locus_tag="VF995_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX9343216.1"
                     /translation="MRLALLLERWPQDPVFDELLGLIAETADAEAGVVVPDVARAFLL
                     AGVAQASGRLAVVVTATTADAESLTEDLAAFLGPERVAVFPAWETLPHERLSPRPETV
                     ARRLALLQRLAGHDPAAPALQVLAVPARAMMQPLAPGLDATRPVRVAAGAQLDLEDLL
                     ERLVAAGYRRTDMVERRGEVAVRGGLVDFFPPGEDHPVRVEFWGDQVEGIRAFAVSSQ
                     RSLTELPEVVAYPCREVRLGEAERNRARQLAHEVPGAGDLLAQVGEGLDVEGVESLLP
                     LLFDQLPLLTSYLPDDALLVLADPKRTLDRAEEVRKQADEAMQSGWGTAAEGATAPVE
                     GAGYHPLDEVLRTAGRAVLRVSPFDPGEETRPASLRIDAHPVEPYRAAIGRVAHDARE
                     LAAAGNTVLLCTEGPGPAKRLVDVLAEEGLTVPLATELPAGADQAHSGGPGQVLVGTA
                     PLLTGFRLPALRLALIGEGDLYGTRRQTREQARMPASRRRSTQAGASIALEELQPGDV
                     VVHNVHGIARYVGMEHRTVAGAERDYLLLEYDQGDRLYLPSEQVEMIRRYVGGEAPKL
                     SRLGSKDWDRQKARVRKKVREMAAELIRLYSARMASPGHRFAPDSPWQRELEDAFPFV
                     ETPDQLTAVEEIKRDMETPVPMDRLLCGDVGYGKTEIAVRASFKAVMDGKQVAVLVPT
                     TLLAQQHFATFSERFAPFPVKVGTLSRFASRKEQDETIAAMANGTVDVVIGTHRLLST
                     DAKWSDLGLVIIDEEHRFGVAHKEHLKQLRTEVDVLTLTATPIPRSLEMSLSGIRDLS
                     VMETPPEQRHPVLTFVGPYDAGTVANAIRREMLREGQTFFVHNRVDSINRVASQIRQL
                     VPEARVGIAHGQMSEEQLERVMLEFWDKQHDVLLCTTIIESGLDIPSANTLVVDRADT
                     LGLAQLYQLRGRVGRSRQRAYAYLFYPPERSITEASHQRLATVATHQDLGSGMAIALK
                     DLEIRGAGNLLGADQSGHVALVGYDLYMQMLSEAVAELSGRPIEQPKELKLEVPVDAN
                     IPAAYVPRERLRLEAYRRLGGARSVEEVEALAAELTDRYGPLPQPVRNLLVLGGVRAQ
                     AAAAGLSEVVCFGGRVRLGPVTRLAESKQVRLDRLYRGALWKQAEQSLFVPLPAGEVN
                     LPEWLSELLEGVLEAPKPPVLPASGRTGGLAAAS"
     gene            complement(12000..12395)
                     /locus_tag="VF995_06335"
     CDS             complement(12000..12395)
                     /locus_tag="VF995_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9343217.1"
                     /translation="MRFADTSFWFALQERRDRHHEAAKALVHAGVGRVCVSNHVVGET
                     WTLLRRRAGHAPAVGFIDRLARLPGVEVVHVDTTLEADAWRWLRQHDEREYSFVDATS
                     FAVMRRRRIHEALAFDDDFSAAGFVEVRP"
     gene            complement(12392..12616)
                     /locus_tag="VF995_06340"
     CDS             complement(12392..12616)
                     /locus_tag="VF995_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX9343218.1"
                     /translation="MRRIQIHIDEALDKAVAEEAARRGISKAALIRASIAREVGAGAS
                     PPEDPWAAMVGWLDDDPVDDIDEVLYGPRG"
     gene            complement(12741..13700)
                     /locus_tag="VF995_06345"
     CDS             complement(12741..13700)
                     /locus_tag="VF995_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX9343219.1"
                     /translation="MVDVTAVRAAAERIAGRVHRTPVVTSATLDGRSGAQVMIKAEAL
                     QKTGSFKPRGALNRLLTLPEDARDRGLIAISAGNHAGAVAWAASQVGAKATVVMPQHA
                     TPAKVAACEGYGAEVVLHGDTTVEAFAECDRLREERGLTLIHPFDDPEIVAGQGTAAL
                     ELVEDAGPLDVWIVPVGGGGLACGTTLAVRDANPACRVIGVEPESAATLSAALAAGEP
                     VPITPRSVADGLCAPYAGPQTFPLFRELVDQVVTVSESELVAAVRFVMERMKLVVEPA
                     GAAAIAALLASKAGDLASARVGTILSGGNLDIAKIAALPDWLD"
     gene            complement(13742..14317)
                     /gene="pth"
                     /locus_tag="VF995_06350"
     CDS             complement(13742..14317)
                     /gene="pth"
                     /locus_tag="VF995_06350"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX9343220.1"
                     /translation="MADAGPWLVLGLGNPDREYGNSRHNAGARVIVRLAEKFGAPLKR
                     SKNRAEVAEVRDGDQRLVLARNSSYMNESGGPASLLVRYYKVPLDRLIVVHDEIDLAE
                     GKLQVRFDGGTAGHNGIKDVAKALGSPDFHRVRLGVGRPPGRQDPADYVLAPIPQRLA
                     EDFGVLIERGADAVLDLVHLGLERAQDRNNR"
     gene            complement(14369..15037)
                     /locus_tag="VF995_06355"
     CDS             complement(14369..15037)
                     /locus_tag="VF995_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX9343221.1"
                     /translation="MAEFRLAAEPRTETGKGAARRIRAAGRVPAVLYGHGAKPKHLTV
                     DAREFSHALRTDAGSNVLIALEVGRSRQLALAKEVQRHAVRGSFIHIDFLVVRRGEKV
                     EVTVPLRLVGEAPGVRGGGVSDQDLYQVHVEAEVTAVPDGIDADISGLGIGDVLRVRE
                     LRVPEGVTILDDPEAPVVSVVTPRVEPEPVAEAEGEAAEGAEGTEGEGAGTEGAGGGE
                     GGGE"
     gene            complement(15251..16327)
                     /locus_tag="VF995_06360"
     CDS             complement(15251..16327)
                     /locus_tag="VF995_06360"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX9343222.1"
                     /translation="MDLPVMPPVRPMLAKLVSIVPEGMLYEPKWDGFRCLAFRDGDEV
                     ELGSRNERPLTRYFPELVAAAREQLPERCVIDGEVVVANGPVLDFEALQQRIHPAASR
                     VNLLAEETPGRFIAFDLLALGDHDLTGRPFAARRALLEEALGGDRAPYYLTPMTRSHK
                     VAERWFRIFEGAGLDGVVAKPPDLLYLPDQRAMFKVKHERTADCVVTGFRWHKSGPVV
                     GSMLLGLYNDQGKLQHVGVTSSFTMARRRELLDELTPYRLGPGDEHPWLGTVGGTRIP
                     GGEPSRWNAKKDLSWEPLAPTQVVEVAYDHMEGLRFRHTAQFRRWRPDRDPASCTYEQ
                     LERPLAFDLGEILAGRGEGQAEIG"
BASE COUNT         2257 a         5740 c         5992 g         2341 t
ORIGIN      
        1 caggtgctca ccaacctgct cgacaacgcc gtcaagtact cccccgacgg tgccccgatc
       61 cggatcgaag cacgacgcga cgccggccag gcggtcctcg ccgttgagga cgccggcccg
      121 ggcgtccccg ccgccgaccg cgagcgcatc ttcgagcgct tcgcccggct ggagcagggg
      181 gaggcccacc gcgccggcgg cgtcggcctg ggcctgtgga tcgcccgcca gctcgcccgc
      241 aacctcggcg gcgacctgct cctcgccccg ccgtcgcggc cggacggcgg ggcccggttc
      301 gagctgcgcc tcccactggt ccagaatgtc ctcggcaccg agcgctcaat gacgctggcg
      361 gcgacgccga gataaccctg gcgccgccct ttcaaggtgc ccgaaccagc gagaagccct
      421 tcttcctggc aatcaggcgc ccaagagggt agcgtgttga tctctgtgcg ggcctagacc
      481 cgccgtttcg agtattcggg gccaaatacg gccgtcctcg tgggaggtgg agcatgatcg
      541 ctgcctgctc gaacgaccta gatgccccgc tcggattcgc cccgcgcagt ccccgacccg
      601 ggtgcgggcg gtcgggagac ggcatccggt agcacccggg gaagcgtcgg cgcgacagcg
      661 tcaccaggat tcagccgcgg cgctcgccga tcaaggaggt cctccagtca tgtctcagtc
      721 aagcgacgaa cgcgtcacct acctcacgcc tgaggcctac gagcgtctga gcacggagct
      781 cgacgagctg aagaccgagg gccgtgagcg tgtctcccag cagatcggcg tggcgcgcga
      841 gcacggggac atccgggaga acgccgacta cgacgcggcc aagaatgagc agggcatgat
      901 ggaggcgcgc atccgccagc tcgagaagct gctgcgctcg gcggtcgtcg gtatcccgcc
      961 cgccaccggc aaggccggcc cgggcatggt ggtcaagctg aacatcgccg gcgacgagga
     1021 gatgtacttc ctcggcagcc gcgaggaggc gatcgacggc atggacgtgc tgagcctgcg
     1081 gtccgccctc ggccgggcgg tggtcggccg cacccccggc gacgagttca gctacaccac
     1141 cccgaccggc cgcgagctcc ccgtcaagct gctcgaagcc cagccgcgcg cctagcctgg
     1201 cctagcgcgg cccgcgccgg cggaggccgc ggtcgatggt gtccgcgccg tcggcggcgg
     1261 acccggtgac gacctcaccc gggaggtggc cagccgcctc agcggcctcg agcagcgcct
     1321 ggccgcccca ccagagccgc gaggaaccat gaacggcacc ggcgccaggg agggcgagcg
     1381 ccaaaagggc actgggccga tcgcgccgga gccagccgat ggtccgctcc agggactccc
     1441 ccatggtgag gctgaccgcc agcccggccg ggtcgagggc cgccgcggcc cgcgccagca
     1501 ccggtcctgg caggcgggga ccgaggtaga ccacatgatg gccgcgcgcc cgcagcagca
     1561 gccgcaggag tagggcgccc agttcgtgct gctcctccgg gccgcaggcg accagcagcg
     1621 gctgaccgcg cccgggcggg aacgcgtcga gcagccgcag cagttcgcgg cggagcacct
     1681 ccgaggcgaa atgctcggcc gccactcggc ccgcaccctc cacgtcaccc gcgccctcgg
     1741 cgatggcgcc gatcgccagc tcgaccaggg cgagggcggc gccctccagg ccgaggagcc
     1801 gcaccgcggc ggcgaagcag cgctcggccc cggcttcgtc gaaggcgccc agcgcccgga
     1861 tcaggcgctc caggagctgc tcggggtcgg cttcggtgcc ggcctggcgc agcagcgcgg
     1921 tcacctgtcc gatggacagg ccctcggcgc gctggcgggc gaggaagcgg atgagagcga
     1981 cgtcgtggtc gctgtagagg cgctggccgg tggcggtccg caggggacgg gggaagccgt
     2041 agcggcgctc ccaggagcgc agcgtgctgg cggggatgcc ggtgcgggcc tcgaccgcgg
     2101 ccacccggta tcggggcgcc aggcggtcag ccgcgccctc cacggtcagt gcgggcggag
     2161 ccgtcggatc cttgtgcgcg tgcggcaccc gggcctcctg gcgaccggtc cctggctggc
     2221 gatcggttcc gcgccagttc gagaagcacg ccatccagga tatctgtttc gctcgctacc
     2281 acccgggcga accggaaggt ccggcagatc tcatccaaaa ccagggctcc ggccgcgata
     2341 acgtcttccc ggccaggcgc catcaccggg agcgcggccc gctgcgcgat ggtgaggcgg
     2401 cagaggcgcc gcgcggtcgc ggcgatccgc ggggcctcca gcacggcgtg gtggatccgc
     2461 tccgggtcgt agtgatccag gtcgagggtg agcgccgcca cggtggtcac cgtgccggcc
     2521 acccccacca cccgcgtggc gctggacgga tcgaacaccg tctggacccg ctccaggtgt
     2581 tcccggacgt ccgcgcgcag ggcggccacc tggtcggcgg tgggcgggtc gctgtggagg
     2641 tggcgctcga acagccgcac gcagccgatc tccagcgaga tcgcggcttc ggggtcgcgg
     2701 ttgccgacga tgaactcggt cgatccgccg ccgatgtcga ccaccagcgt gcgggcatcg
     2761 ccgtcaaggt caccggtggc gccgaggtag gaggcggccg cctcctgctc gccggtcagc
     2821 acctccgcct ccacgccgac cagccggcgg acgccgtcga ggaactcctc ccggttggcg
     2881 acgtcgcggg tcgcgctggt cgccaccagg cggcgccgct gtggcccgac cccgaaccgg
     2941 tcgcaggtgg cggcgtactc ccccagcacc tccagggtgc gccgcagcgc accggggtcg
     3001 aagcggcggt tggcgtccac gcccttgccg agccgggtga tgatcatctc ccggtcgaac
     3061 tgctcgctga tgcgcccgtc atcgacgtcg gccaccagca gccgcgtcga attggtccca
     3121 acgtcgaacg ctgccagcct catccgtcca gatcctccac acagggtcca gggcagtcga
     3181 tcgggtcgat caccgggcga atcatacggc ccacggggtc ggcgccggtg gcggcctcgt
     3241 gggcgtagag cacgtggagg cacttcaccc ggccggggag gccgccgacg ccggggtcgc
     3301 caggcaggcg gccgagggcg tcgcgctggg cgaggtagct ttcgtgggcg gcggcgtgcg
     3361 cctcggcgag ggccgggtcg gcggcgaccc gctcgctcag ggcggcgttc cagcccgccg
     3421 cttccagacg gctgacggcg accctggcgg ccgggcaggc gagccagtac agggtcggga
     3481 agggggtgcc gtcgtcgagg tacggcgcgg tgcggatcac cgcggggagg ccgtagcggc
     3541 aacgggccgc gaccgcgatg gcgccgcgga cgcggcggcc gatctgcgcc tcgatcaccc
     3601 gccgctcggc agcggtggcg tcggggaggc cggcagccca ctcacgttcg ccccgggggt
     3661 cggcgccacc cgtggggggc acgggcgact cccgctccca ggggagcgtg cgcgggtcag
     3721 gcagcacgct cggacgtcgg ggggaccggg acggggtgca cggcacaggt caggtacgcc
     3781 ggccctcgcg ctggcgcttg acatccacca ggcgctcttc gctggagcgc atgaacttct
     3841 tgagcttctg ctcaaattca gggtccttgg ccgcccggcg gcggggtgcc tcgggctgcc
     3901 agtccggcgc tgcctgtttg atggacaggt cgatcttgcc gtcctccttg acgcccagga
     3961 ctttcaccat gacctggtcg tcaaggcgga ggtgctcctc aaccgagcgg acatagttcc
     4021 ggtcgatctc ggagatatgg acaagaccgg tctcaccaga ctcgagcttt atgaaggccc
     4081 catacggtgc gatgcgaacg accgtgcccg ggacgactgc cccgatctcg gcactcatac
     4141 tggcgactga cgctccttta ttctctcgtc ccccacgatc cactgcttcg ctgacaggaa
     4201 acacccaatg cgaagctcac aacgagacgg tcgggaggaa ctggcggcga gccggagttg
     4261 acaggctctc tgttcatcct agggacggcc tggcagagcg tcaacaaagg cacatgctcc
     4321 ggcacgatcc gaaagatgcc gcagtgctgg cgggactagt ggctcgttcc ggtcagccag
     4381 cgccaaaacc gcgtgtacca gggtggtttg ggcggcggtt tgggcgcggg cggcggcttg
     4441 gcggctggcg gggtgccggt gatctcccac tgctgctcgc ccggcctgac cacgttcagg
     4501 ttctgcttgg cgagctgttc caggtaggcg ggatccttca ggcgtgcccg ctcggcggcg
     4561 agctgcgcgt tggcggcgtc cagggcggcc tgcttggcct gaagctgccg gatccgcgcc
     4621 tgctgggaga cgtgctcgcg cagcggatag accgcggtga ccgcgagcag gaacaccacc
     4681 aggaccagca ccacccctcg cggggtgagg ccgaccctgc gtccgcccac gcccgccgcg
     4741 agggcggctc tcatggcgtt tcgcctcccg gcgggaagct gcgcgggaag gcggtccgcc
     4801 ccgggtagcg ggcggcggcg ccgagggtct cctcgatgcg cagcagctgg ttgtacttgg
     4861 cgacccgctc gctgcgggcg ggggcgccgc tcttgatctg gccgcagccg agcgccaccg
     4921 ccagatcggc gatcgtggtg tcctcggtcg cgcccgaacg atggctcacg accgtggtcc
     4981 agccgccgcg gtgggcgagc tcgacggcgt cgaaggtctc ggtcagggtg ccgatctggt
     5041 tgaccttgac cagcaccgag ttgccgacgc cttgcgccag gccacggcgg atgcgggtca
     5101 cgttggtgac gaagatgtcg tcgccgacca gttgcacccg gtcccccagt gcctcggtga
     5161 gggcggccca gccgtcccag tcgtcctcgg cgaggccgtc ctcgatcgac acgatcgggt
     5221 aacggtcgca cagctcggcg tacagctcaa ccatcccggc gctgtcgagc gagcgtccct
     5281 ccgcggtcag ctggtagcgg ccgtcgcggt agaactcgct ggaggccggg tccagggcga
     5341 gcgcgacctc cggccccggc tcgtagccgg cctggccgat cgcctcgacc agcagctgga
     5401 gggcggcctc gttgccgggc aggttgggcg cgaagccgcc ctcgtcgccg acgccggtgg
     5461 acagcccctt gcccttcagg accgatttga gggtgtggta gacctcagcg cccatccgca
     5521 gcgcctcgcg gaacgagggc gcgccaaccg ggacgatcat gaactcctgg aagtccacct
     5581 cgttgtcggc gtggacgccc ccgttgagca cgttcatcat cggcaccggc agcaggtggg
     5641 cggccatgcc gccgaggtgg cgaaacagcg ggatgccgag cgagtcggcc gccgcccggg
     5701 ccaccgccat cgacaccccc agggtggcgt tggcgccgag cttggacttg ttgtcggtac
     5761 cgtccaggtc cagcagcagg tggtcgatgg cgcgctggtc ctcgccgtcc tcgtccagca
     5821 ggctcggggc gatcacctcg ttgacgtggc ggacggcgcc aaggacgccc ttgccgccgt
     5881 agcgggtctc tccgtcgcgc agctcgaccg cctcgaacct gccggtggag gcgcccgacg
     5941 ggacgatcgc gcgccccagg ccgccgttgt cgagctgcac ctcgcactcg agggtcgggt
     6001 tgccccggga atcgaggact tcgcgtgcga ggaccagctc gatcgatgcc atagggaaag
     6061 tcgctccggc ggtgtcggga atttggttga tcgcgaggat atcagctgtc gtgctcgcgg
     6121 agccgttccc tgaaccgctc gcgatactgg cggctgacgg tccgcagcgc cgcctcgggc
     6181 tcgacgtcca gggcgagcgc caggccgacc acgctgaaca gcagctcgcc gatccgctgc
     6241 tggcgcccct cggcgatccc cgcgaccgca gggccctcca acccccccag ggcggcgcgg
     6301 acgtcccgtc gcgcctggtc gatgtcgggt ccatggccgg cggccttcct gcgcagcttg
     6361 gcggcgaact gcagggccgg cagggccttg gggatgccgg cgaacgggtc gccccggccc
     6421 tcctcctcgc gcttgatccg ctcccagttg gagaccacct cgtcggacgt ggcgacctgg
     6481 aggtcgccga agacgtgggg gtggcggcgg tgcagcttct cggtgatcgc gcgggcgacc
     6541 ccgtcgatgt cgaagctgcc ggcgtcctcg gcgatctggg cgtggaacac cacctgcagc
     6601 agcaggtcgc ccagctcctc gcgcaggtgc tcggggtcgg cgtccgcgtc gtcgatcgcc
     6661 tcgaccagct cgtaggcctc ctcgaccagg tggcgggcaa gggaggcgtg ggtctgctcc
     6721 cggtcccagg ggcagccgcc aggaccgcgc aggcgcgtct cggtggcgac caggtccagc
     6781 agggctgacc ccttcggctc tcccagcggg tagaccgcct cgacctcgac gtgctggcgt
     6841 gccgcccggt cgcacaccag ctggaccagc gggccgtcat gctccggcgg cagcagcaga
     6901 gtggcggagc cgcgctcctt ggcgagggcg agcagcgcgt cggcctgccg ccgtgccagc
     6961 gccacgccct ccacgtcaag gccctggccg accagcagca ggtcgcggcc ggccggcccg
     7021 gtgtcgccgg cgggcgccag gacctcccag gggatctccc ccacctcaag ggccggcagc
     7081 gagggatgct ggtcgtcgag caggtacacc ggcttggcgg cgtccagggc ccgccacgtc
     7141 tgtggcgccc acagcaacgg gagccgcggg ctggccgcga ccaggacgac gcgggtcatc
     7201 ggcggcggcc caggctcagg aggtcaccgg gacgctgctg ctggagttgg tgtaggcacc
     7261 gggcgcggtg ctgcggtcga ccacatggcc gcttgcctgg tcccacttgc cgaagcgcgg
     7321 gttgaccgtg acggcggcgt ccttgagcag gcccttgaac cagctgtcga ccgcggtctg
     7381 gcggcgggtg gtgatgttcg ggtcgagctt ctggcgcagg tcgtcggtga gctgcggcag
     7441 ccgcacgccg gtcacctgga tgacgtggta gccgtactgg ctcttgaccg gggcggagat
     7501 cggcgaggcg cacgacccgg aggtcttgcc cttgcagttg ccctggttgg cgagggaggt
     7561 ggcggccttc tcgaactcgg ccacgaaggt gcccttggcg gcgaagccga ggtctccgcc
     7621 cttgtccttg ctgcccgggt cgatcgagta cttcttggcc acggcgctcc agtcgccgcc
     7681 cttgatcagc tggtcgtgca cggtgttggc ggtcttctgg tccttgacca gcacgtggcg
     7741 gacgtggacc tccaggtagt ccgacttgtg ctggttcatg atccgctgca gcacgtcgtc
     7801 ggcctggtag gggaccagct tggtggccac ggagttgacc gcgagctgga cgcggagctg
     7861 ctggcgcagc agcgcgtcgg tgaggttgtt ctgggccagc agggcctcgt aggtggtgcc
     7921 ctgctgctgg gcctgggctt tgatggtgtt gacgcggccg tcgatgtccc cgtcggtgac
     7981 ggcgacgccc ttctgcttgg ccccgtcaag gacgatctgg aactggatca gcccttcgag
     8041 ggactgctgc gtgagcgtga ccatgtcctg cgcggtcgac gcggtcgact gggacgcggc
     8101 gccaagcttg gcgctcacct cctccttgag ctggctgacc gggatgatgg tgccgttgac
     8161 cacggcggcc tggctggagc tgctcttgcc gcagccactc gcgaccaggg caaacagcgc
     8221 ggcgaggcac aacgcgaccg gaacgccggt cagggatctg cggcgcaaga ggaacctcga
     8281 tttgggatcg gcaatcacaa ccgcaccaga atagcgccgt ccactcatct aggccctccc
     8341 tacgaggctg ccgcgaggcc gccggtccgg cctgacgcgg gcagcaccgg cggcttgggc
     8401 gcctccagca cgccctccag cagctccgag agccactcgg ggaggttcac ctctcccgcc
     8461 gggaggggga cgaacaggct ctgctcggcc tgcttccaca gggcgccgcg gtagaggcgg
     8521 tcgaggcgga cctgcttgga ctcggccagc ctggttaccg ggccgagccg gacgcggccg
     8581 ccgaagcaga ccacctcgct gagcccggcc gccgccgcct gggcccgcac cccgccgagc
     8641 accaggaggt tgcgcaccgg ctgcggcagc ggcccgtagc ggtcggtcag ctcggcggcg
     8701 agcgcctcga cctcctcgac cgagcgggcg ccgccgaggc ggcggtaggc ctccagccga
     8761 agccgctcgc gtggcacgta ggcggcgggg atgttggcgt cgaccgggac ctccagcttg
     8821 agctccttcg gctgctcgat cggccggccg ctcagctcgg ccaccgcctc cgacagcatc
     8881 tgcatgtaga ggtcgtagcc gaccagcgcg acgtggcccg actggtcggc gccgagcagg
     8941 ttgccggcgc cgcggatctc caggtccttg agcgcgatcg ccatgccgga gccgaggtcc
     9001 tggtgggtcg cgacggtcgc cagccgctgg tgggacgcct cggtgatgga gcgctctggc
     9061 gggtagaaca ggtaggcgta ggcgcgctgc cgggagcgcc cgacccggcc gcggagctgg
     9121 tagagctgcg ccaggccgag ggtgtcggcg cggtccacga ccagggtgtt ggcgctcggg
     9181 atgtccaggc cagactcgat gatcgtggtg cacagcagca cgtcgtgctg cttgtcccag
     9241 aactccagca tcacccgttc cagctgctcc tcgctcatct ggccgtgggc gatgccgacg
     9301 cgcgcctcgg gcaccagctg gcggatctgg gaggcgaccc ggttgatcga gtcgacccgg
     9361 ttgtggacga agaaggtctg gccctcgcgt agcatctcgc ggcggatcgc gttggcgacc
     9421 gtgcccgcgt catacggccc gacgaaggtc agcaccggat ggcgctgctc gggcggcgtc
     9481 tccatcaccg acaggtcgcg gatgccggac aggctcatct ccagcgagcg cgggatcggc
     9541 gtggcggtca gggtgagcac gtccacctcg gtgcggagct gcttgaggtg ctccttgtgg
     9601 gcgacgccga agcggtgctc ctcgtcgatg atcaccaggc cgaggtcgga ccacttggcg
     9661 tcggtggaca gcagccggtg ggtgccgatc accacgtcga cggtgccgtt ggccatcgcc
     9721 gcgatggtct cgtcctgctc cttgcgggag gcgaagcgcg acagcgtccc gaccttgacc
     9781 gggaagggcg cgaaccgctc gctgaaggtg gcgaagtgct gctgggcgag cagggtggtc
     9841 ggtaccagca cggcgacctg cttgccgtcc atcaccgcct tgaagctggc acggaccgcg
     9901 atctcggtct tgccgtagcc gacgtcgccg cacagcagcc ggtccatcgg caccggcgtc
     9961 tccatgtcgc gcttgatctc ctcgaccgcg gtcagctggt cgggggtctc cacgaacggg
    10021 aaggcgtcct ccagctcccg ctgccagggc gaatcgggcg cgaagcgatg cccgggggaa
    10081 gccatccgtg cgctgtagag ccggatcagc tcggcggcca tctcgcgcac cttcttgcgg
    10141 acgcgggcct tctggcggtc ccagtccttg ctgccgagcc gcgacagctt cggcgcctcg
    10201 ccgccgacgt agcggcggat catctcgacc tgctcgctcg gcaggtagag gcggtcgccc
    10261 tggtcgtatt cgagcagcag gtagtcgcgc tcggccccgg cgacggtgcg gtgctccatg
    10321 ccgacgtagc gggcgatccc atggacgttg tggaccacga cgtcgccggg ctgcagctcc
    10381 tccagcgcga tcgatgcgcc cgcctgggtg gagcgccgcc ggctggcggg catccgcgcc
    10441 tgctcgcggg tctgccgccg ggtcccgtag aggtcgcctt cgccgatcag cgcgaggcgc
    10501 agcgccggca gccggaaccc ggtcagcagc ggcgcggtcc cgaccagcac ctggcccggg
    10561 ccgccgctgt gggcctggtc ggcgccggct ggcagctcgg tggcgagcgg gaccgtcagg
    10621 ccctcctcgg cgagcacgtc gaccagccgc ttcgccgggc cgggcccctc ggtgcacagc
    10681 agcacggtgt tgccggcggc ggccagttcg cgggcgtcgt gggcgacccg cccgatggcc
    10741 gcgcggtagg gctcgaccgg gtgcgcgtca atccggaggg aggccgggcg cgtctcctcc
    10801 ccgggatcga aggggctcac ccgcaggacc gcccgcccgg cggtgcgcag gacctcgtcg
    10861 agcgggtggt agccggcgcc ctccaccggg gcggtcgccc cctcggcggc ggtcccccac
    10921 ccggactgca tcgcctcgtc ggcctgcttg cggacctcct cggcgcggtc gagggtgcgc
    10981 tttgggtcgg ccagcaccag cagcgcgtca tccgggaggt agctggtcag cagcggcagc
    11041 tggtcgaaca gcagcggcag cagcgactcc acgccctcga cgtcgagccc ctcccccacc
    11101 tgggcgagca ggtccccggc gcccggcacc tcgtgggcga gctgccgggc gcggttgcgt
    11161 tccgcctccc cgagccgcac ctcccggcag ggataggcga ccacctcggg cagttcggtg
    11221 agggaccgct ggctcgacac cgcgaacgcg cggatcccct ccacctggtc gccccagaac
    11281 tcgacccgga cggggtggtc ctcgccgggt gggaagaagt cgaccagccc gccgcggacc
    11341 gccacctcgc cgcgccgctc gaccatgtcg gtgcgccggt agcccgccgc gaccagccgc
    11401 tccagcagat cctccagatc gagctgggca ccggccgcga ccctgaccgg ccgggtggcg
    11461 tcgaggcccg gcgccagcgg ctgcatcatc gcccgcgcgg gcaccgccag cacctggagt
    11521 gcgggcgccg ccgggtcgtg gcccgccagc cgctggagca gcgccagccg ccgcgccacc
    11581 gtctcgggcc ggggcgagag ccgctcgtgc ggcagcgtct cccaagcggg gaacaccgcc
    11641 acccgctccg gcccgagaaa cgcggccagg tcctcggtga gcgactccgc gtccgccgtg
    11701 gtcgcggtca ccacgaccgc cagccgccca ctcgcctgcg ccacccccgc cagcaggaac
    11761 gcccgcgcca cgtcgggcac gaccaccccg gcctcggcgt cggctgtctc cgcgatcagg
    11821 ccgagcagct cgtcaaagac cggatcctga ggccaccgct caagcaggag ggcaagacgc
    11881 acgaatttca acctctctca accacgcaca aggacccccc aaccaaacga ctggggggtg
    11941 gaacctacat gcctagcgta acgcagcgag gcgacaaccc attccgagcg ggttgctcat
    12001 catggacgga cctcgacgaa cccggcggcg ctgaaatcgt cgtcaaaggc gagcgcctcg
    12061 tggatccggc ggcggcgcat gacggcgaag ctggtcgcat cgacgaagga gtactcccgc
    12121 tcgtcatgct gacggagcca gcgccaggca tcggcctcga gcgtggtgtc gacgtggacg
    12181 acttcgacac cgggcagtcg cgcgaggcgg tcgatgaatc caactgccgg cgcatgcccg
    12241 gcacggcggc gcagcagcgt ccacgtctcg ccgacgacgt ggttggagac gcacacacgc
    12301 cccaccccgg cgtggaccag cgccttcgca gcttcgtgat ggcggtcgcg ccgctcctgg
    12361 agcgcgaacc agaagctggt gtcagcgaac ctcatcccct gggcccgtag agaacctcgt
    12421 cgatgtcgtc gacggggtcg tcgtccagcc agccgaccat ggcggcccag ggatcttccg
    12481 gaggtgaggc accggcgccg acctctcgcg cgatggacgc tcggatgagg gcagccttgg
    12541 agatgccacg gcgggcggcc tcctcagcga ccgccttgtc gagcgcctcg tcgatgtgaa
    12601 tctggatcct ccgcatgacc acgatcatac acaccggcag cgccgagagt gtgtatgatc
    12661 gcggtacgtg gccgcccaca cgtcgagcat gccctgctcg aagggttcga ccgctggaaa
    12721 ccgataggcc ggggccgccg ttagtcgagc cagtccggga gagcggcgat cttggcgatg
    12781 tcgaggttgc cgccagagag gatggtaccg acgcgggcgc tggcgagatc gccggccttg
    12841 ctggccagca gggccgcgat ggcggcggca ccagcgggtt ccaccaccag cttcatgcgt
    12901 tccatgacga agcggacagc ggcgaccagc tcagattcgg agacggtcac cacctggtcg
    12961 accagctcgc ggaacagcgg gaaggtctgc gggccggcgt agggggcgca gaggccgtcg
    13021 gcgaccgacc ggggggtgat cgggacgggc tcacccgcgg ccagggcagc gctcagggtg
    13081 gccgcggatt cgggctccac cccgatcacc cggcaggccg ggttggcgtc gcggacggcg
    13141 agggtggtgc cgcaggcgag gccgccgcca ccgacgggga cgatccagac gtcgagcggg
    13201 ccggcgtcct cgacgagttc gagggcggcg gtgccttggc cggcgacgat ttcggggtcg
    13261 tcgaaggggt ggatcagggt gaggccgcgc tcttcgcgga ggcggtcgca ttcggcgaag
    13321 gcttcgacgg tggtgtcgcc gtgcaggacg acctcggcgc cgtagccttc gcaggcggcg
    13381 accttggcgg gggtggcgtg ctggggcatg accacggtgg ctttggcgcc gacctggctg
    13441 gcggcccagg cgacggcgcc ggcgtggttg ccggcggaga tggcgatcag gccgcggtcg
    13501 cgtgcgtcct cggggagggt gagcaggcgg ttgagggcgc cgcggggctt gaaggagccg
    13561 gtcttctgga gggcctcggc cttgatcatg acctgggcgc cgctgcggcc gtcgagcgtc
    13621 gccgaggtca cgaccggcgt ccggtggacc cgcccggcga tccgttccgc tgccgcccgc
    13681 accgccgtca cgtccaccat gcgccccctt gcgccgcgtt ccgccgttct gcttggcgac
    13741 cttagcggtt gttacggtcc tgcgcccgtt ccaggccgag gtggaccagg tcgaggaccg
    13801 cgtcggcgcc gcgctcgatc agcacgccga agtcctcggc gaggcgctgg gggatcgggg
    13861 ccaagacgta gtcggcgggg tcctggcggc cgggcggtcg gccgacgccg aggcggacgc
    13921 ggtggaaatc gggtgagccg agcgccttgg ccacgtcctt gatgccgttg tggccggcgg
    13981 tgccgccgtc gaagcggacc tggagcttgc cctcggccag gtcgatctcg tcgtggacca
    14041 cgatcaggcg gtccagcggg accttgtagt accggaccag caacgaggcg ggcccgccgg
    14101 actcgttcat gtagctggag ttgcgggcga gcaccaggcg ctggtcgccg tcgcggacct
    14161 cggcgacttc ggcgcggttc ttggagcgct tcaagggcgc gccgaatttc tccgccaggc
    14221 gcacgatcac ccgcgcgccc gcgttgtggc ggctgttgcc gtattcgcgg tcagggttgc
    14281 ccagcccgag caccagccac gggccagcgt cggccatgtc ggatcgctcc tggtcgcggc
    14341 ggcggaaggg aaggaagggc agggcgggtt actccccgcc gccctcgccg ccaccagcac
    14401 cctcggtccc ggcgccctcg ccctcggtgc cctctgcgcc ttcggcggcc tccccctcag
    14461 cctcggcgac cggctcgggc tcgaccctcg gcgtcaccac ggagaccacc ggcgcctcgg
    14521 ggtcgtccag gatggtgacg ccctcgggga cccgcagctc gcggacgcgc aggacgtcgc
    14581 cgatgccgag cccggagatg tccgcgtcga tcccgtcggg cacggcggtc acctcagcct
    14641 cgacgtggac ctggtagagg tcctggtcgc tgaccccgcc cccgcgcacg ccgggcgcct
    14701 cgccgaccag gcgcagcggg acggtgacct cgaccttctc gccgcggcgg acgaccagga
    14761 agtcgatatg gatgaagctg ccacgcaccg cgtggcgctg gacctccttg gccagggcga
    14821 gctggcggga cctgccgacc tccagggcga tcagcacgtt gctgccggcg tcggtgcgta
    14881 gcgcgtggct gaactcgcgc gcgtccacgg tgaggtgctt gggcttggcg ccgtggccgt
    14941 acaggaccgc gggcaccctc cccgccgctc ggatccggcg ggcggcgccc ttcccggtct
    15001 cagtgcgcgg ctctgccgcc agtcggaact cggccatcga aggtctcctt acaaactcca
    15061 ggtccaggat gtggtgcgga tgacgccgaa gacagcgcca aatagccccc tgcggggcga
    15121 aagcgggggc gagcgtagca gtccggccgc gccgtcctca acccgagagc tgcgctggcc
    15181 ccggcggcgt gttggccggg tcactgaggc tggccgggag ggttcccgct cgggcggctt
    15241 ggaacctcgc tcagccgatc tcggcctggc cctcgccccg gccggcgagg atctcgccca
    15301 ggtcgaaggc gagcgggcgt tcgagctgct cgtaggtgca ggaggcgggg tcgcggtcgg
    15361 ggcgccagcg gcggaactga gcggtgtggc ggaagcggag gccctccatg tggtcgtagg
    15421 cgacctcgac cacctgggtg ggggcgagcg gttcccagga gaggtccttc ttggcgttcc
    15481 agcgggaggg ctcgccgccg gggatgcggg tgccaccgac cgtgcccagc cagggatgct
    15541 cgtcgccggg accgaggcgg tagggggtca gctcgtcgag cagctcgcgt cgccgcgcca
    15601 tcgtgaacga ggaggtcacg ccaacgtgct gcagcttgcc ctggtcgttg tagaggccga
    15661 gcagcatcga gccgaccacc gggccgctct tgtgccagcg gaagccggtc accacgcagt
    15721 cggcggtgcg ctcgtgcttg accttgaaca tcgcccgctg gtcggggagg tagagcaggt
    15781 cgggcggctt ggcgaccacc ccgtcgagtc cggcgccctc gaagatccgg aaccagcgct
    15841 cggcgacctt gtgggaacgg gtcatcgggg tcaggtagta cggcgccctg tccccaccga
    15901 gggcctcctc cagcagggcc cggcgggccg cgaagggccg tccggtcagg tcgtggtcgc
    15961 cgagggcgag caggtcgaag gcgatgaagc gccccggtgt ctcctcggcg agcaggttga
    16021 cgcgcgaggc ggcggggtgg atgcgctgct gcagcgcctc gaagtcgagc accggcccgt
    16081 tggccaccac cacctcgccg tcgatcacgc agcgttccgg gagttgctcg cgggcggcgg
    16141 cgaccagttc ggggaagtag cgggtcaggg gccgctcgtt gcggctgccc agctccacct
    16201 cgtcgccgtc gcggaaggcg aggcagcgaa acccgtccca tttcggttca tagagcattc
    16261 cttccggcac gatcgacacc agcttggcga gcatcggccg taccggcggc atcaccggta
    16321 gatccatacc
//
LOCUS       DASWDC010000196         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_715930, whole genome shotgun sequence.
ACCESSION   DASWDC010000196 DASWDC010000000
VERSION     DASWDC010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715930"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..1022)
                     /locus_tag="VF995_06365"
     CDS             complement(243..1022)
                     /locus_tag="VF995_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9343223.1"
                     /translation="MKGRVRFELLLGQVLEALPPAPARVVDVGGGTGQLAVALARRGY
                     QVTVVDISPAMLATCAQRAAGEGEEVSAKVATLQGDATQVAGMLGPASQDAALCHEVV
                     SCVEDPGAVLAALGQVLRPGGVLSLTFVNRDWLVLRAGRQGAFPAALRLLDKPVNPGD
                     EPAAAPAPGAGTGTGTGTAVTLDELERQLARAGFEPAGAAGVSVFAEARHDDLDSAEL
                     TQLVELERRVAGREPYRSSSHIVHLVGKRASDGSSLPRYTR"
     gene            complement(1065..1559)
                     /gene="folK"
                     /locus_tag="VF995_06370"
     CDS             complement(1065..1559)
                     /gene="folK"
                     /locus_tag="VF995_06370"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX9343224.1"
                     /translation="MTRAHVALGSNLGDRMAILDTAVRALDADQQTHVMAVSQVYETE
                     PVGGPEQDLYLNAVAVLETDRDPVALLDLLLATEDRLGRVRAERWGPRTADLDLILYD
                     GPPIETPELTVPHPRARERAFVLVPLVDADPFATFPDGEAAVEALARLAPVSGVVAVG
                     ELET"
     gene            complement(1556..2452)
                     /gene="folP"
                     /locus_tag="VF995_06375"
     CDS             complement(1556..2452)
                     /gene="folP"
                     /locus_tag="VF995_06375"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9343225.1"
                     /translation="MPVESVSPVAPHDLGVGDRTLPTSRRCLVMGVLNVTPDSFSDGG
                     RYLDHRAAVAHGLQLAAEGAGLLDVGGESTRPGAAYVGEAEERARVLPVIDSLARATD
                     VPISIDTRKAGVARAAVAAGAAIINDVSAGRDDPDLFGVAAQAKVPLVLMHMQGTPAT
                     MQDNPHYDDVVAEVETFLAERCAAAEAAGVAHDALVIDPGIGFGKRDPHNYALLANLA
                     RLTRLGHPLLVGTSRKGFVGRALQLPVHDLVEGTAATVVWAVERGARIVRVHDVRHML
                     RVVRMTEALLSAERAEPAGARP"
BASE COUNT          345 a          953 c          922 g          326 t
ORIGIN      
        1 cggctggcgc ggtggtgagg ccggccgcct cggcccgttc gcggccctgg tcggagcgga
       61 ccccgacggt cacctccgcg ccgagccgtt gcaggaagac cgcgagcgcg aggccaaccc
      121 gcccggcgcc gatcaccgcc gccggtgagt acttcctgat gggttcctct ggcataggag
      181 ctccgatcgc cggatccggc cacccgctcg gctgttggga gcgacggccg gacaccgcca
      241 gctcagcgag tatatcgagg caagctggac ccatccgatg cccgcttgcc tactaaatgg
      301 acgatatgcg aactcgaccg gtacggctcg cgaccggcga cccgccgctc cagctccacc
      361 agctgggtca gttcggcgct gtccaggtcg tcatggcgtg cctcggcgaa gacgctcacc
      421 ccggcggcgc ccgccggctc gaagcccgct cgggccagtt ggcgctccag ctcgtcgagg
      481 gtcaccgcgg tcccggtccc ggtcccggtc ccggcgccgg gagcgggcgc cgccgctggc
      541 tcgtcgccgg ggttgactgg cttgtccagc aggcgcagcg cggccgggaa ggcgccctgc
      601 cggccggcgc gcagcaccag ccagtcgcgg ttgacgaagg tgagcgacag caccccaccg
      661 ggccgcagca cctgcccgag ggcggccagc acggcgccgg ggtcctcgac gcagctcacc
      721 acctcgtggc agagggcggc gtcctggctc gcggggccga gcatgccggc gacctgggtg
      781 gcgtcgccct gcagggtcgc gactttcgcg ctcacctcct cgccctcgcc ggcggcgcgc
      841 tgggcgcagg tggccagcat cgccggggag atgtccacca cggtcacctg atagccgcgc
      901 cgcgccagcg ccaccgccag ctgcccggtg ccgccgccga cgtccaccac ccgcgcgggc
      961 gccggtggca gcgcctcgag cacctgcccg agcagcaact cgaagcggac gcgccctttc
     1021 atggcctgtc cctcgtcatg tccgtcacgt gcgcgtccat tacgtcacgt ctccagctcc
     1081 cccacggcga ccacgcccga aaccggggcg agccgggcca gcgcctcgac cgcggcctcg
     1141 ccgtccggga aggtggcgaa cgggtcggcg tcgaccagcg gcaccagcac gaaggcgcgc
     1201 tcccgcgcgc gcgggtgcgg cacggtcaac tctggcgtct cgatcggggg cccgtcataa
     1261 aggatgaggt cgaggtcggc ggtgcgcggg ccccagcgct cggcgcgcac gcgcccgagg
     1321 cgatcctcgg tggcgagcag caggtccagc aacgcaaccg ggtcacggtc ggtctccagc
     1381 accgccaccg cgttcaggta gaggtcctgc tccggaccgc cgaccggttc ggtctcgtag
     1441 acctgggaga ccgccatcac gtgggtctgc tggtcggcgt ccagcgcccg gaccgccgtg
     1501 tcgaggatcg ccatccggtc ccccaggttg gagccgagcg ccacatgcgc cctcgtcatg
     1561 gccgtgcccc cgctggttcc gcccgctccg cggacagcag cgcctcggtc atgcggacca
     1621 cgcggagcat gtggcggacg tcgtggacgc ggacgatgcg ggcgccccgc tcgaccgccc
     1681 agacaaccgt ggccgccgtg ccctcgacca ggtcgtggac gggcaactgc agggcgcggc
     1741 cgacgaagcc cttgcggctg gtgccaacca gcagggggtg gccgagacgg gtgaggcggg
     1801 cgaggttggc gagcagggca tagttgtgcg ggtcgcgctt gccgaagccg atgccggggt
     1861 cgatcaccag ggcgtcgtgg gccaccccgg cggcctcggc agcggcgcag cgctcggcga
     1921 ggaaggtctc gacctcggcg acgacgtcgt cgtagtgggg gttgtcctgc atcgtggcgg
     1981 gggtgccctg catgtgcatg agcaccagcg ggactttggc ctgggccgcg acgccgaaca
     2041 ggtcggggtc gtcgcggccg gcgctgacat cgttgatgat cgccgcgcca gccgccacgg
     2101 cggcccgggc gacgcccgcc ttgcgggtgt cgatcgagat cggcacgtcg gtggcccgcg
     2161 ccagcgaatc gatcaccggc agcaccctgg cccgctcctc cgcctcgccg acgtaggcgg
     2221 cgccggggcg ggtcgactcg ccgccgacgt ccagcaggcc agccccttcg gcggcgagct
     2281 gcagcccgtg cgccaccgcg gcgcggtggt ccaggtagcg gcccccgtcc gagaacgaat
     2341 ccggcgtgac gttgagcacc cccatgacca ggcagcgccg cgatgtcggc agcgtccggt
     2401 caccgacacc cagatcatgc ggggcgaccg gcgacaccga ctcgacaggc acaggccaac
     2461 ctccaggaac agggcgtccg acggccggtg cgagatcggc tcgcttgacc ggcggggcca
     2521 tgatagctgc gtcggccact ccccca
//
LOCUS       DASWDC010000197         6094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1465803, whole genome shotgun sequence.
ACCESSION   DASWDC010000197 DASWDC010000000
VERSION     DASWDC010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465803"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1271
                     /locus_tag="VF995_06380"
     CDS             45..1271
                     /locus_tag="VF995_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011771046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX9343226.1"
                     /translation="MAAPGPMAVDWEERVDFRRLHTYRLARARQALDASELGAVLAFD
                     MNNVRYLTSTHIGEWARDKLITFALLARGAEPVIWDFGSAAKHHRLYCPWLDPANSRA
                     GLTGLRGAVSPDAGLFERAAREVKEELNRAGVGDLPLGVDVAELPLLFELQRLGVEVR
                     DGQQVMLDARQVKSRDEIMLLTQAAAMGDAVYQHIAEALKPGIRENEIVALASKVLYD
                     LGSDDVEAINAISGERCSPHPHNFSDRLIRPGDQAFFDIIQAFHGYRTCYYRTFNVGR
                     ATPAQRDAYDRAREWMDAAIELVKPGVTTDQIARLWPGAQDFGFADEMEAFGLQFGHG
                     LGLGLHERPIISRLNSLDHPAEITEGMVFALETYCPAADGYSAARIEEEVVVTADGVQ
                     VITLYPAETLPIANPY"
     gene            1326..2321
                     /locus_tag="VF995_06385"
     CDS             1326..2321
                     /locus_tag="VF995_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX9343227.1"
                     /translation="MPAEIEIDRETRLALWRLMVELRLFEQRAHDLFLQNLVKGTSHL
                     ALGQEAIAAAFGVAMRPDDWTFCTYRGHAHTLARGASMEAAMAELLGRATGLLGGKGG
                     SMHLGSAEHSAMGSYAIVGAHLPVANGAAWSAQYRGSGQVAVCFFGDGATNIGAFHEA
                     LNLAATWKLPVVFCCENNLYMEYTPIASVTAVPRPAADRAAAYGLEAVAGDGNDADVM
                     YRMAVAALRKARDGGGPSLIEAATYRKGGHSRADPANYRPDDEVAAWMARDPLALYRQ
                     RLLDLGVEEAELAAIERAALAAVDAATEAAKAAPEPSPETLETDLWSDGSSAWRN"
     gene            2309..3286
                     /locus_tag="VF995_06390"
     CDS             2309..3286
                     /locus_tag="VF995_06390"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX9343228.1"
                     /translation="MAELITYREAVTRGIAQEMGHDERVVFLGEDVAAPGGVFKTTPG
                     LLEKFGPTRVRDTPISEQAITGAAMGAAMTGLRPIAEIMFADFLATCWDIVANQIAKT
                     RYMSGGQVTLPLVIRTAQGAGLRFGAQHSQSVENWGMAIPGLKVVAPSTPADVIGLLA
                     AAVRDDDPVLFCEHKGLYASKGEVPDGELVDTLGSAKVLRAGDDVTLLALAAMVPRAL
                     AAAEILAGHGVEATVVDVRSLVPLDTRTILEAAAHTGRVFTVEENPRLCGWGAELVSI
                     IAEECFWDLDQPIVRITTPHIPLPSAAALEDHAVPTVDRIVATVSEAGT"
     gene            3357..4421
                     /locus_tag="VF995_06395"
     CDS             3357..4421
                     /locus_tag="VF995_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9343229.1"
                     /translation="MGDGRAPRLKDVAVAAGVHVSTASRALNEQTTGLVNPETTERVR
                     QAAHALGYRINGMARALKTRRSLAIGMLVPDITNPFFPPIVRGAEDAFAEAGYSLVLS
                     NTDNDPDKARRQLAAMLETRVDGLLLATALRNDELVAELAASDTPVALVNRTVDADAD
                     GATVSAAVPDDHLGVALAVEHLYQLGHRRIAYLGGPLDVSNGHRRRVSFDQAARRLGL
                     DQAVAVEAAAFNEAEGRRGAAALLGHALDANGAGSARPTAIMAGNDLMALGVLDAAAA
                     RGLACPRDLSVVGFNDMPLADRMRPPLTTVRVAEYDLGRRAAELLLATIDNPRRSPET
                     VLLAPELVVRGSTGPAPSSS"
     gene            4497..5708
                     /locus_tag="VF995_06400"
     CDS             4497..5708
                     /locus_tag="VF995_06400"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9343230.1"
                     /translation="MQSVAASVADERVRAAAVNWAPRFVANGTDSTDVQATLARIERW
                     PEWCRQWGRTAAHYEALAERAEQRGRHRTAAGAWRRAGLCWHWGKFLFVDHPDEQRAA
                     HERAVAGYQRGAWALDPPAERVEIPYPDEEFRLAAYLRLPAAGHPIGELPPGHLGSPD
                     AVGEASLARPPHPQPPPVVVMAPGLDSVKEELQATADYFLERGLATLAIDGPGQGEAE
                     YDLPIEPAYERVVAAACDWLEARPGIDPARIAVFGVSLGGYYAARAAAFEPRLRAAVA
                     LAGPYRFDREFERLPSLTRAAFQRRSGAASPEEAKERAAALSLEGVAERITIPLLALF
                     GKLDRLIHPSEADQLAAVAPGGELVMFEDGNHGLTNHVYESRTLMADWLAEHLAPDAE
                     PNAGPAADASG"
     gene            5695..>6094
                     /locus_tag="VF995_06405"
     CDS             5695..>6094
                     /locus_tag="VF995_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343231.1"
                     /translation="MRAAEAEAAAGPGPDRGPGEGARGWGVDVHAHCIPAAVLDTLER
                     DSGRYGIELIDWDGVRGARIAGRVMTGPLRPDLIDTDMPRRLKAMDQAGVRVQLLSSF
                     AELTAYALDPPAGARYARMFNEALAASAAAH"
BASE COUNT          855 a         2384 c         2079 g          776 t
ORIGIN      
        1 cggccgtggc ggacagcgac accaacgcag cggcggcgcg gcggatggcc gcgcccggcc
       61 cgatggcggt cgactgggag gagcgggtcg acttccgccg cctccacacc tatcgcctcg
      121 ctcgcgctcg ccaggcgctg gacgcgagcg agctgggcgc ggtgctggcc ttcgacatga
      181 acaacgtccg ttacctgacc tcgacccaca tcggcgagtg ggcgcgcgac aagctgatca
      241 ccttcgccct gctggcccgc ggcgccgagc cggtcatctg ggacttcggc tccgccgcca
      301 agcaccaccg cctctactgc ccctggctcg acccggccaa ctcccgcgcc gggctcaccg
      361 ggctgcgcgg cgcggtcagc cccgacgccg ggctgttcga gcgcgccgcc cgcgaggtca
      421 aggaggagct gaaccgcgcc ggggtcggcg acctgccgct cggggtggac gtcgccgagc
      481 tccccctgct gttcgagctg cagcgcctcg gtgtcgaggt gcgcgacggc cagcaggtca
      541 tgctcgacgc ccgccaggtc aagtcccgtg acgagatcat gctgctcacc caggccgccg
      601 ccatgggcga cgcggtctat cagcacatcg ccgaggcgct caagcccggc atccgcgaga
      661 acgagatcgt cgcgctcgcc tccaaggtcc tctacgacct cggctccgac gacgtcgagg
      721 cgatcaacgc gatcagcggc gagcgctgca gcccccatcc ccacaacttc tccgaccgcc
      781 tgatccgccc cggcgaccag gcgttcttcg acatcatcca ggcgttccac ggctaccgga
      841 cctgctacta ccgcaccttc aacgtgggcc gcgccacccc cgcccagcgc gacgcctacg
      901 accgcgcccg cgagtggatg gacgcggcca tcgagctggt caagcccggc gtcaccaccg
      961 accagatcgc tcggctgtgg cccggcgccc aggacttcgg cttcgccgac gagatggagg
     1021 cgttcggcct ccagttcggc cacggcctcg gcctcggcct ccacgagcgg ccgatcatca
     1081 gccgcctgaa ctccctggac cacccggccg agatcaccga gggcatggtc ttcgcgctgg
     1141 aaacctattg tcccgccgcc gacggctatt ccgccgcccg tatcgaggaa gaggtcgtgg
     1201 tgaccgccga cggcgtccag gtgatcaccc tctacccggc cgagaccctg ccgatcgcca
     1261 acccctattg accgcctgac catcgaggtg tcgaggccgt ggctgaaacc gagactcgca
     1321 acacgatgcc cgccgagatc gagatcgacc gcgagacccg gctggcgctg tggcggctga
     1381 tggtggagct gcgcctgttc gagcagcgcg cccatgacct gttcctgcag aacctggtca
     1441 aggggaccag ccacttggcg ctcgggcagg aggcgatcgc cgccgcgttc ggggtggcga
     1501 tgcgccccga cgactggacc ttctgcacct accgcggcca cgcccacacc ctggcgcggg
     1561 gcgcctccat ggaggccgcc atggccgagc tgctcggccg cgccaccggc ctgctcggcg
     1621 gcaagggcgg ctccatgcac ctcggcagcg ccgagcactc cgccatgggg tcgtacgcga
     1681 tcgtcggcgc ccacctgccg gtcgccaacg gcgccgcctg gtcggcgcag taccgcggca
     1741 gcgggcaggt ggcggtgtgc ttcttcggcg acggcgccac caacatcggc gccttccacg
     1801 aggccctcaa cctggccgcc acctggaagc tcccggtggt gttctgctgc gagaacaacc
     1861 tctacatgga gtacaccccg atcgcctcgg tgacggcggt gccgcgcccc gccgccgacc
     1921 gcgccgccgc ctatgggttg gaggcggtgg cgggcgacgg caacgacgcc gacgtgatgt
     1981 accgcatggc ggtcgcggcg ctgcggaagg cccgcgacgg cggcggcccc tccctgatcg
     2041 aggccgccac ctaccgcaag ggcggccact cccgcgcgga cccggcgaac taccgccccg
     2101 acgacgaggt cgccgcctgg atggcccgcg acccgctggc cctgtaccgc cagcgcctgc
     2161 tcgacctcgg cgtcgaggag gccgagctgg ccgcgatcga acgggcggcg ctggccgccg
     2221 tcgacgccgc caccgaggcg gccaaggccg caccggagcc cagccccgag acgctggaga
     2281 ccgacctgtg gagcgacggg agctcagcat ggcggaactg atcacctatc gggaggcggt
     2341 gacacgcggc atcgctcagg agatggggca cgacgagcgg gtggtatttc tcggcgagga
     2401 cgtcgccgca ccaggcgggg tcttcaagac caccccgggc ctgctggaga aattcggccc
     2461 gacccgggtg cgcgacaccc ccatctcgga gcaggccatc accggcgccg ccatgggcgc
     2521 cgccatgacc gggctgcggc cgatcgcgga gatcatgttc gccgacttcc tggcgacctg
     2581 ctgggacatc gtcgccaacc agatcgccaa gacccgctac atgagcggcg ggcaggtcac
     2641 cctcccgctg gtgatccgca ccgcccaggg cgcggggctg cgcttcggcg cccagcactc
     2701 ccagagcgtg gagaactggg gcatggcgat ccccggcctc aaggtggtcg cgccctccac
     2761 ccccgccgac gtgatcggcc tgctcgccgc cgccgtccgc gacgacgacc cggtgctgtt
     2821 ctgcgagcac aagggcctct acgccagcaa gggcgaggtc cccgacggcg agctggtcga
     2881 caccctcggc agcgccaagg tgctgcgcgc cggcgacgac gtcaccctgc tcgccctggc
     2941 cgcgatggtg ccccgagcgc tcgccgccgc cgagatcctc gccggccacg gcgtcgaggc
     3001 gacggtggtg gacgtccgct cgctggtgcc gctcgacacc cgcaccatcc tggaggccgc
     3061 cgcccacacc ggccgggtct tcacggtgga ggagaacccg cgcctgtgcg gctggggcgc
     3121 cgagctcgtc tcgatcatcg ccgaggagtg cttctgggac ctcgaccagc cgatcgtccg
     3181 catcaccacc cctcacatcc cgctcccctc ggccgccgcc ctggaggacc acgcggttcc
     3241 caccgtggac cggatcgtgg caaccgtcag cgaagcgggc acgtgacagc ccgctgaact
     3301 caggtactat ccctggaaca gcggtcacta gacggttcgg gttcggggga gcaagggtgg
     3361 gggacggccg cgcaccaagg ctgaaagacg tcgcggtcgc cgccggcgtg catgtgtcga
     3421 cggcgtcgcg ggcgctgaac gagcagacca ccgggctggt caatcccgag acgaccgaac
     3481 gggtccgcca ggccgcccac gcgctcggct accggatcaa cggcatggcc cgcgcgctga
     3541 agacgcgccg gtcgctggcg atcgggatgc tggtccccga catcaccaac ccgttcttcc
     3601 cgccgatcgt gcgtggcgcc gaggacgcct tcgccgaggc cggctactcg ctggtgcttt
     3661 ccaacaccga caacgacccg gacaaggcca ggcgccagct cgcggcgatg ctggagaccc
     3721 gggtggacgg cctgctgctc gccaccgccc tgcgcaacga cgagctggtc gccgagctgg
     3781 ccgcctcgga caccccggtc gcgctggtca accggaccgt cgacgccgac gccgacggcg
     3841 cgacggtcag cgcggccgtc cccgacgacc acctcggcgt cgccctcgcc gtggagcatc
     3901 tctaccagct cggccaccgg cgcatcgcct acctcggcgg ccccctcgac gtctccaacg
     3961 gccaccgccg ccgcgtcagc ttcgaccagg ccgcgcggcg gctcggcctc gaccaggccg
     4021 tcgcggtcga ggcggccgcc ttcaacgagg ccgaggggcg gcgcggggcg gcggccctgc
     4081 tcggccacgc ccttgacgcg aacggcgccg ggtccgcccg gcccaccgcg atcatggccg
     4141 gcaacgacct gatggcgctc ggcgtgctgg acgccgccgc ggcccgcggc ctggcgtgcc
     4201 cacgcgacct gtcggtggtc ggcttcaacg acatgccgct cgccgaccgc atgcgcccgc
     4261 cgctcaccac cgtccgggtc gccgagtacg acctcggacg ccgcgccgcc gagctgctgc
     4321 tcgccacgat cgacaacccc aggcgctcgc cggagacggt cctgctcgcc ccggaactgg
     4381 tcgtccgggg ctcgaccggg cccgctccct ccagctcctg acaggctcct cagcgtcacc
     4441 tgacggccca ccgccaggag cttgacaagc gggaggcagc ggggcatggt cacgcaatgc
     4501 aatcggttgc tgcctcggtg gcggacgagc gagtccgggc agcggcggtc aactgggcgc
     4561 cgcggttcgt cgccaatggc accgactcca ccgacgtgca ggccaccctt gcccgtatcg
     4621 agcgctggcc ggagtggtgc cggcagtggg gccgcaccgc cgcccactac gaggcgctcg
     4681 ccgagcgggc cgagcagcgc ggccgccacc gcaccgccgc cggggcctgg cggcgcgccg
     4741 gcctctgctg gcactggggc aagttcctgt tcgtcgacca ccccgacgag cagcgcgccg
     4801 cccacgagcg cgcggtcgcc ggctaccagc ggggagcatg ggcgctcgac ccgcccgccg
     4861 agcgggtcga gatcccctat cccgacgagg agttccggct ggccgcctac ctgcgcctcc
     4921 cggccgccgg ccaccccatc ggggagctac cgccgggcca tctggggtct ccggacgctg
     4981 tgggcgaggc ctcgcttgcc cgcccccccc acccccagcc cccgccggtg gtggtgatgg
     5041 cccccggcct cgactcggtc aaggaggagc tgcaggcgac tgccgactac ttcctggagc
     5101 gcggcctcgc caccctcgcc atcgacgggc ccggccaggg agaggccgaa tacgacctgc
     5161 ccatcgagcc cgcctacgag cgggtcgtgg cggccgcctg cgactggctg gaagcgcggc
     5221 ccggcatcga tcccgcccgg atcgccgtct tcggcgtcag cctcggcggc tactacgccg
     5281 cccgcgccgc cgccttcgag ccgcgccttc gcgccgccgt cgcgctcgcc gggccgtacc
     5341 gcttcgaccg cgagttcgag cggctcccgt cgctgacccg cgcggccttc cagcgccgct
     5401 ccggcgcggc cagccccgag gaggcgaagg agcgcgccgc cgccctcagc ctggagggcg
     5461 tggccgagcg gatcaccatc ccgctgctgg cgctgttcgg caagctcgac cgcctgatcc
     5521 acccgtcaga ggctgaccag ctcgccgccg tggcacccgg cggcgagctg gtcatgttcg
     5581 aggacggcaa ccacggcctc accaaccacg tctacgagtc gcggacgctc atggccgact
     5641 ggctggccga gcatctcgcc ccggatgcgg aaccgaacgc gggaccggct gcggatgcga
     5701 gcggctgagg ccgaggccgc cgcggggccg gggccggatc ggggtccggg cgagggtgcc
     5761 cgtggctggg gcgtcgacgt ccacgcccac tgcatcccgg cggcggtgct cgacacgctg
     5821 gagcgcgaca gtggccgcta cggcatcgag ctgatcgact gggacggggt gcgcggcgcc
     5881 cgcatcgccg ggcgggtgat gaccggcccg ctgcgacccg acctgatcga caccgacatg
     5941 ccccgccgcc tcaaggcgat ggaccaggcc ggtgtgcgcg tccagctgct ctccagcttc
     6001 gccgagctca ccgcctatgc cctcgacccg cccgccgggg cccgctacgc ccgcatgttc
     6061 aacgaggccc tggccgccag cgccgccgcg cata
//
LOCUS       DASWDC010000198         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1050470, whole genome shotgun sequence.
ACCESSION   DASWDC010000198 DASWDC010000000
VERSION     DASWDC010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050470"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..877
                     /locus_tag="VF995_06410"
     CDS             89..877
                     /locus_tag="VF995_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9343232.1"
                     /translation="MSGPALHGKVVVVTGAAQGIGRAYARRLVAEGCQVITADRAHDR
                     VLEAAAQLGDRAEGGEGGKAVPRHLDVADERSCEALAAEVMDTYGHLDGLVNNAAIFS
                     TITMKPFWELSVGEWDAVLAVNLRGPWLLTKALVPALRASGSSGGASVVNVASDAVWQ
                     GRAGYLHYITSKAGILGMTHAMAHELGEANIRVNSISPGSVYTEVPRATVTEAQKAAM
                     LAAQALKRPAGPDDLAGTVVFLLSGDSAYITGQTIHVNGGLIHR"
     gene            complement(864..2078)
                     /locus_tag="VF995_06415"
     CDS             complement(864..2078)
                     /locus_tag="VF995_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX9343233.1"
                     /translation="MTGDERRWQPGAGSAPEPDQNQEQDQAPEQGELRVSSLELFFDL
                     VFVFTLTQLTTALYTHLSAVGALRVVLVFAVLWWIYGGYAWLTNSVPPTSHARRLLLI
                     AGMAAFLVIALAIPEAFGGAGVAFGIAYLAVVVVHGALYTLAMPLRGVVRFSGPNVVA
                     SLLLVVAGLIHSDGTAGSALRYGLWIAAVLIEFVTPYVLALDAPLRIQVGHFVERHGL
                     LLLIAFGESVVAIGIGIQTGAGQALNLELVGAALLGLALAAALWWSYFGGDEERAERA
                     LRAAPAELQPRLALSAYFFAFIPMLLGIVTIAVGVKSSIGHLTRPLHAGPAVALGTGV
                     ALYLIGDLLFRRALRSGPVAARAAAALLALASIPLGLVAAVAQLLALVALLAATLLAE
                     AALTRLNGLTGG"
BASE COUNT          372 a          817 c          775 g          251 t
ORIGIN      
        1 agaccttctg cccgtcgtgc ctggtcctgc tggccaccga gatcgcccca gccgacgagg
       61 ccgcctaccg gcgctggagc gtcaacccgt gagtgggccc gccctccacg gcaaggtcgt
      121 ggtcgtcacc ggcgccgcgc aggggatcgg ccgcgcctac gcgcgccggc tggtcgccga
      181 gggctgccag gtgatcacgg ccgaccgtgc ccacgaccgc gtgctggagg cggccgccca
      241 actcggggat cgcgccgagg gcggcgaggg cggcaaggcc gtgccgcgcc acctcgacgt
      301 cgccgacgag cggagctgcg aggccctggc cgccgaggtc atggacacct atgggcacct
      361 cgacggcctg gtcaacaacg ccgcgatctt ctccaccatc accatgaagc cgttctggga
      421 gctgtccgtc ggcgagtggg acgcggtgct ggccgtcaac ctgcgcggcc cgtggctgct
      481 caccaaggcg ctggtcccgg cgctgcgggc cagcggatcg agcggcggcg ccagcgtggt
      541 caacgtcgcc tcggacgcgg tgtggcaggg ccgcgccggc tacctccact acatcaccag
      601 caaagcgggc atcctcggca tgacccacgc gatggcccac gaactgggcg aggccaacat
      661 ccgggtgaac agcatctccc ccggctcggt ctacaccgag gttccccgcg cgacggtcac
      721 cgaggcgcag aaggccgcga tgctcgctgc ccaggccctg aaacgcccgg cgggccccga
      781 cgacctcgcc ggcacggtgg tgttcctgct ctccggcgac agcgcctaca tcaccggcca
      841 gacgatccac gtcaacggcg gcctcatcca ccggtgagcc cgttgagccg cgtgagggcc
      901 gcctcggcga gcagcgtcgc cgccagcagg gccaccagcg cgagcagctg cgcgaccgcg
      961 gcgaccaggc cgagcgggat cgaggcgagg gcgagcagcg cggcggcggc gcgagcggcg
     1021 accgggccgc tgcgcagcgc ccggcggaac agcaggtcgc cgatcaggta gagggcgacg
     1081 ccggtgccca gcgcgaccgc cgggccggcg tggagcggcc gggtgaggtg gccgatggag
     1141 ctcttcaccc cgacggcgat ggtgacgata ccaagcagca tcgggatgaa ggcgaagaag
     1201 taggcgctga gcgcgagccg gggttgcaac tcggcgggag cggcgcgcaa cgcccgctcg
     1261 gcgcgttcct cgtcgccgcc gaagtacgac caccaaagcg cggcggcgag tgccaggccg
     1321 agcagcgccg cgccgaccag ctccaggttg agggcctggc cggcgccggt ctggatgccg
     1381 atcccgatcg ccaccaccga ctcgccgaag gcgatcagca gcagcagacc gtgccgctcg
     1441 acaaaatgcc cgacctggat gcggaggggc gcgtcgagcg ccagcacgta cggcgtcacg
     1501 aattcgatca gcaccgccgc gatccacagc ccgtagcgca acgcactgcc cgccgtgccg
     1561 tccgagtgga tcagccccgc caccaccagc agcaacgacg ccaccacgtt gggtccggaa
     1621 aagcgcacga ccccccggag cggcatcgcc agggtgtaga gcgcgccgtg caccaccaca
     1681 accgccaggt aggcaatccc gaaggcgacc ccggcgccgc cgaacgcctc cgggatcgcc
     1741 agcgcgatca ccaggaaggc ggccatcccg gcgatcagca gcaaccgccg cgcgtggctg
     1801 gtcggcggga ccgaattcgt gagccaggca tacccgccgt agatccacca gagcaccgcg
     1861 aagaccagca ccacccgcag cgcccccacc gccgacaggt gggtgtagag cgccgtggtg
     1921 agctgcgtca gcgtgaagac gaaaaccagg tcgaagaaca gctccaggct cgaaacccgc
     1981 agctccccct gttccggcgc ctggtcctgc tcctggttct ggtcaggttc gggcgctgac
     2041 ccggccccgg gctgccagcg gcgctcgtcc cctgtcatgg ttcacaatct agcgtccggc
     2101 aacgacagca ggacaaggga gctttccgct atgagcgtac cgacccagac tgtcgaggtg
     2161 accctgccgg acggccggcg gctggaggcg ctgaccgcgg gcccggcgga ctccg
//
LOCUS       DASWDC010000199         6751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1111632, whole genome shotgun sequence.
ACCESSION   DASWDC010000199 DASWDC010000000
VERSION     DASWDC010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111632"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1413
                     /locus_tag="VF995_06420"
     CDS             106..1413
                     /locus_tag="VF995_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018851075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX9343234.1"
                     /translation="MPYLDYAASAPLRAEALEAMRPFLEAPAANPSSQHGPGRAARHA
                     VETAREQVAALVGADPAEVVFTSGGTEADNLAVKGTVLARRDDPAAARGLVASAVEHH
                     AVLDAAAWAEADAGAEVRLAPVDSQGRVLLDRLGALLGPGRTALVAVMAANNEVGTLQ
                     PVAATAELAHAADAVLLCDAVQAAGLPGVDLRRDGIDLLALAAHKLGGPTGVGALVIR
                     AGTTTPRPLLHGGGQERGLRSGTLPVAAIAGFGAAAAAALTERERGHGDPGFQVEGVA
                     HPGPAVALRALRDRLLAGLRGAAPSLQVNGDPSAAGGLPGLLSARFPGVRAEDLLLLL
                     DRAGVACSAGSACASGAVSASHVLLAMGRTPEEARETIRFSLGHGSTATDVDAAVAAV
                     RQALDVLAPELPTGGRPLGRPGSPDAMVGASLLRPPNPPGASR"
     gene            1410..2543
                     /gene="mnmA"
                     /locus_tag="VF995_06425"
     CDS             1410..2543
                     /gene="mnmA"
                     /locus_tag="VF995_06425"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX9343235.1"
                     /translation="MSGPQVLVAMSGGVDSSVAAALLAEAGCDVTGVHLKLAKTPAHE
                     QVKGKGCCTLADADDARRVAATLGIPFYVWDFAEQFEREVVADFEAEYARGRTPNPCA
                     RCNEQVKVAGLLARARALGFDALATGHYARVSDGRLYKGADEAKDQSYVLYMLGPDQL
                     AHLRLPLGELTKPEVRATAARLGLRVAQKPESFDVCFIPDGDTAGWLERRLGPQPGEV
                     VDTAGRALGRHQGAYRYTVGQRRGLGIAVPTPRYVLRVEPAAARVVVGERADLAVGTV
                     ELERVTTTDGRPLRPGPARARLRAHGPEVACQVVPLGLGDRRARLDLDEPITAVAPGQ
                     AGVLYDGDLVLGGGTIAATSAARPAAAEARAAGDANGRAEAGA"
     gene            2597..3571
                     /locus_tag="VF995_06430"
     CDS             2597..3571
                     /locus_tag="VF995_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343236.1"
                     /translation="MPHADADACVAAALRICPDLPFWPTRPLAVGDEQMIRQWALGLP
                     GVGDAPGEDKGGGGVAWVGRDRAPGQPALAHSAAGTLQPLLRALRQRPGPPGWLKLQA
                     TGPVTLAVALDAGGRAAISIPSARGPLALGYAARLRSVVTRVREALPAWKLLVVLDEP
                     SLTHPLVAAEPKLAVELWQSARSTGADVTGLHCCATPPWGLLLDLDPQLVSFDAVTHG
                     DDATDDPAFRRLIASGTAVAWGLIPSAPEVGSDAEAEALKADPEALAARLLDLVGWTA
                     GDYRPEVLARSLVTPTCGLAALDEPEADRRLTLAAATGRVAWEQAGLP"
     gene            complement(3613..4476)
                     /locus_tag="VF995_06435"
     CDS             complement(3613..4476)
                     /locus_tag="VF995_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003968813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9343237.1"
                     /translation="MSRSSRQRPGPGPGPGPGPGPGPGAEWVRAWKPAVAGVHEVFHA
                     RFVDYAYPPHVHDAWTVFLIDDGGIRYDLERRHRGAGAGGDLVGLLPPHVVHDGRAAS
                     RSGFRKRVLYLATDVLDERLIGPAVDQPDVHEPALRRAVQALHRALGQPDDALEAEAR
                     LALVGERLRAHLGDLPASAGAGDRPPARLARDLRDLLDGRLFEATTLAQAGSALGASP
                     AHLVRSFTRTFGIAPHQYLVARRIDAARGRLLAGEPIALVAAGVGFFDQAHFSRQFKR
                     HVGTPPGRYTL"
     gene            4527..5117
                     /locus_tag="VF995_06440"
     CDS             4527..5117
                     /locus_tag="VF995_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX9343238.1"
                     /translation="MLNELLGLAGFALVVSITPGPNNTLLLASGIQFGFTRTLRHVAG
                     TAMGMAVLIFVMAAGAGVVLLAVPGADLALKAIGSAYLLYLAFGIASSHGASRTAVSR
                     PLGVLNGAAFQFANPKAWLFALSAASTFLPRGLAPAAAALAVAATCAVVILPTASVWA
                     AGGAALSRIVADGRTQRAISIALALILAASVAFIWI"
     gene            complement(5131..5946)
                     /locus_tag="VF995_06445"
     CDS             complement(5131..5946)
                     /locus_tag="VF995_06445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9343239.1"
                     /translation="MPKRAVELVVTDLDGTLCDASECIHPRVIAAVHQLEAAGIPVLI
                     ATGRRPRATAWVLEPGGIPGPAVMLDGAIGRDLRDGSVFHEAVFPPEGASRVLAAFVA
                     AHLSPSLFVERPGVEMINGPRPDTHPGHLARNRAWAVRDDLERVVRTEPVRTIAVVGG
                     DGARLAEVAATIDPTAGSAAVTPDLLYGGHTLQVRPPGTSKWSGVLSFCAAHGLDPTR
                     VLAVGDGNNDLELLDAAAVPVAVTGGSPTALARARHVIDPPSAGGWAAVLDLV"
     gene            complement(6038..>6751)
                     /locus_tag="VF995_06450"
     CDS             complement(6038..>6751)
                     /locus_tag="VF995_06450"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9343240.1"
                     /translation="QLTGSAAFLVDYRQSLAARLPLVLGVIGLATMLLLWLMTGSVVV
                     PLKAVVMNVLSLGASFGALVWVFQEGHLSGLLGFEPTGTVDITVPVLVFVFAFGLSMD
                     YEVFLLSRIKETWDESGDNDHAVALGLQRTGRIVTSAAALIVAVFLGFASGELLTIKE
                     IGVGMALAIVIDATVVRTLLVPAAMKLMGRWNWWSPTSLRRLHDRLGLLRETPAAPAP
                     APAPAPPAEAPAPEPVHPG"
BASE COUNT          817 a         2652 c         2450 g          832 t
ORIGIN      
        1 cacccagtgc cacacttggc tgagcgaacg gccaaggcgc ctggctggac caacggtccc
       61 gggccgcgag ccacgccgaa ccttccggag gcttcaggag gacccgtgcc gtatctggac
      121 tatgccgcca gcgccccgct ccgcgcggag gcgctcgaag cgatgcggcc gttcctggag
      181 gcgccggccg ccaacccctc cagccagcac gggccaggcc gcgccgcgcg ccacgcggtc
      241 gagaccgccc gcgagcaggt cgccgccctg gtcggcgccg acccggccga ggtggtcttc
      301 acctccggcg gcaccgaagc cgacaacctc gccgtcaagg gcaccgtgct ggcccgccgt
      361 gacgacccag ccgccgcgcg cgggctggtc gccagcgcgg tcgagcatca cgccgtgctc
      421 gacgcggccg cctgggcgga ggccgacgcc ggcgccgagg tccgcctcgc accggtggac
      481 tcccaggggc gggtgctgct cgaccggctc ggcgcgctgc tggggccggg ccgcaccgcg
      541 ctggtcgcgg tgatggccgc caacaacgag gtcgggaccc tccagccggt cgccgccacc
      601 gccgagctgg cccacgccgc cgatgccgtg ctgctctgcg acgcggtcca ggccgccggc
      661 ctgccaggcg tcgacctgcg ccgcgacggg atcgacctgc tcgccctcgc cgcccacaag
      721 ctcggcggcc ccaccggcgt cggcgccctg gtgatccgcg cgggcaccac cacgccacgc
      781 ccgctgctcc acggcggcgg gcaggagcgc ggcctccgct ccggcaccct cccggtcgcg
      841 gccatcgccg ggttcggcgc cgccgcagcc gcggccctca ccgagcggga gcgaggccac
      901 ggcgatccag ggttccaggt ggagggcgtg gcccatcccg gacccgccgt ggcgctccgc
      961 gccctccggg atcgactcct cgccgggctc cgcggcgccg cccccagcct ccaggtcaac
     1021 ggcgatccca gcgcggccgg cgggctgccg ggcctgctct cggcccgctt ccccggggtc
     1081 cgcgccgagg acctgctgct gctgctggac cgcgccggcg tggcctgctc ggcggggtcg
     1141 gcctgcgcca gcggcgccgt cagcgcctcc catgtcctgc tcgccatggg ccgcaccccc
     1201 gaggaggccc gcgagacgat ccgtttcagc ctcggccacg gctccaccgc caccgacgtg
     1261 gacgccgccg tggcggcggt ccgccaggcc ctcgacgtgc tggccccgga gctgcccacc
     1321 gggggccgac cgctcggccg tcccgggtcg ccggatgcga tggtcggggc ctcgcttctg
     1381 aggcccccga accccccggg ggcctcccgg tgagcggccc gcaggtcctg gtggcgatga
     1441 gcggcggggt cgactcgtcg gtggcggcgg cgctgctggc cgaagccggc tgcgacgtca
     1501 ccggggtgca cctcaagctc gccaagacgc ccgcccacga gcaggtcaag ggcaagggct
     1561 gctgcaccct cgccgacgcc gacgacgccc gccgggtggc ggccaccctc gggatcccgt
     1621 tctacgtctg ggacttcgcc gagcagttcg agcgcgaggt ggtcgccgac ttcgaggccg
     1681 aatacgcccg tggccgcacc cccaacccct gcgcccgctg caacgagcag gtcaaggttg
     1741 cggggctgct ggcccgcgcc cgcgcgctcg gcttcgacgc gctcgccacc ggccactacg
     1801 cccgggtctc ggacggacgc ctctacaagg gcgctgacga agccaaggac cagtcctacg
     1861 tgctctacat gctcggcccc gaccagctcg cccatctgcg cctgccgctc ggcgagttga
     1921 ccaagccaga ggtccgcgcc accgccgccc gcctcggcct gcgggtcgcc caaaagcccg
     1981 agtcgttcga cgtctgcttc atccccgacg gcgacaccgc cggctggctg gagcgccgcc
     2041 tcggtcccca gcccggcgag gtggtcgaca ccgcgggacg ggccctcggc cgccaccagg
     2101 gcgcctaccg ctacaccgtc ggccagcgcc gcggcctcgg catcgccgtc cccacccccc
     2161 gctacgtcct gcgggtcgag cccgccgccg cccgcgtcgt ggtcggcgag cgcgcggacc
     2221 tcgccgtcgg cacggtggag ctggagcgcg tcaccaccac cgacggccgc ccgctgcgtc
     2281 ccggccccgc ccgcgcccgc ctgcgcgccc acggccccga ggtcgcctgc caggtcgtcc
     2341 ccctcggcct cggcgaccgt cgcgcccgcc tcgacctcga cgagcccatc accgccgtcg
     2401 cccccggcca ggccggcgtc ctctacgacg gcgacctggt cctcggcggc ggcacgatcg
     2461 ccgccaccag cgcggctcgg ccagccgcgg cggaggccag ggcggcgggg gacgccaacg
     2521 ggcgcgccga ggccggcgct tgagcgcggt cccgcggtcg ttccggcctc gcggtgccgg
     2581 aaccggcgtc ggcggcatgc ctcacgccga cgcggacgcc tgcgtcgccg ccgccctgcg
     2641 catctgcccc gacctgccgt tctggccgac gcgtccgctc gcggtcggtg acgagcagat
     2701 gatccgccag tgggccctcg gcctccccgg cgtcggcgac gcgcccggcg aggacaaggg
     2761 cggcggggga gtggcctggg tcggccggga ccgcgccccc ggccagccgg ccctcgcgca
     2821 ttcggcggcc ggcaccctgc agcccctcct gcgggcactc cgccagcgcc ccggcccgcc
     2881 cggctggctg aagctccagg ccaccggccc ggtcaccctc gccgtcgcgc tcgacgcggg
     2941 cgggcgcgct gcgatcagca tcccctccgc ccgagggccg ctcgccctcg gctacgccgc
     3001 tcgcctccgc tcggtggtca cccgcgtgcg cgaggccctt cccgcctgga agctcctcgt
     3061 ggttctcgac gagccctccc tcacccatcc gctggtcgcc gccgagccca agctcgccgt
     3121 ggagttgtgg cagtccgccc ggtccaccgg cgccgacgtc accggcctgc actgctgcgc
     3181 caccccaccc tggggcctcc tcctcgacct cgacccccag ctcgtctcct tcgacgccgt
     3241 cacccacggc gacgacgcca ccgacgaccc cgccttccgc cgcctgatcg cctccggcac
     3301 cgcggtcgcc tggggcctca tcccctccgc ccccgaagtg ggctcggacg ccgaagccga
     3361 agccctcaaa gccgacccgg aggccctcgc cgcccgcctc ctcgacctgg tcggctggac
     3421 cgccggcgac taccgccccg aagtgctcgc ccgttcgctg gtcaccccga cctgcggcct
     3481 cgccgccttg gacgagccgg aggccgaccg ccggctcacc ctcgccgccg ccaccggccg
     3541 ggtcgcctgg gagcaagccg gtctcccata ggcgccattc gaccggtcgg cttgattcgg
     3601 gcatgctcag cgtcatagcg tgtagcggcc tgggggcgtg ccgacgtggc gtttgaactg
     3661 gcggctgaag tgggcctggt cgaagaaccc gaccccggcc gcgaccaggg cgatcggttc
     3721 gccggcgagg aggcgcccgc gggcggcgtc gatgcggcgg gccacgagat actgatgcgg
     3781 cgcgatcccg aaggtgcgcg tgaaggacct gacgaggtga gccggggagg cacccagggc
     3841 tgatcccgcc tgcgcgagcg tggtggcttc gaacagccgc ccgtcgagca ggtcccgcag
     3901 gtctcgcgcc agcctggccg gggggcggtc cccggcgccg gcgctggcgg ggagatcgcc
     3961 caggtgggcc cgcagccgct cgccgaccag tgcgagccgg gcctcggcct ccagcgcgtc
     4021 gtcgggctgg ccgagcgcac ggtgcagcgc ctggacggcg cggcgcaggg ccggctcgtg
     4081 gacgtccggc tggtccacgg ccgggccgat caagcgctcg tccagcacgt cggtcgccag
     4141 gtacaggacc cgcttgcgga acccgctgcg gctggcggcg cggccgtcat gcacgacgtg
     4201 cggtggcagg aggccgacca gatcgccgcc ggccccagct ccccggtggc ggcgctccag
     4261 gtcgtagcgg atgccgccgt cgtcgatgag gaacaccgtc caggcgtcgt ggacgtgcgg
     4321 cgggtaggcg tagtcgacga agcgggcgtg gaagacctcg tggacgcccg cgaccgccgg
     4381 cttccacgcc cggacccact ccgctcccgg tccgggtccg ggtccgggtc cgggtccggg
     4441 tccgggtccg ggtcgctgcc gggatgaccg cgacatggca aaaatcgtac aagaccagac
     4501 tccgccgggc gtcgtccact ggacgcatgc tgaacgagct gcttggactg gccggcttcg
     4561 cgctggtcgt ctcgatcacg cccggaccca acaacacgct gctgctggcc tccgggatcc
     4621 agttcgggtt cacccggacc ctgcgccacg tcgccgggac cgccatgggc atggccgtcc
     4681 tgatcttcgt catggccgcg ggggccgggg tcgtgctgct ggcggtaccc ggcgccgatc
     4741 tcgccctcaa ggcgatcggc tcggcctacc tcctctacct cgccttcggg atcgccagca
     4801 gccacggggc cagccgcacg gccgtctcgc ggccgctcgg cgtcctgaat ggcgccgcct
     4861 tccagttcgc caaccccaaa gcgtggctgt tcgccctcag cgcggccagc acgttcctgc
     4921 ccaggggcct ggcgccggcg gcggcggcgc tggcggtcgc ggccacgtgc gcggtcgtga
     4981 tcctcccaac cgcgtcggtc tgggccgccg gcggcgccgc cctcagccgc atcgtcgccg
     5041 acgggcgcac ccagcgggcg atcagcatcg cgctcgccct catcctggcc gcctcggtcg
     5101 ccttcatctg gatctgacgc ttcgcagcgg tcataccagg tcgaggaccg ctgcccagcc
     5161 gccggccgaa ggtgggtcga tgacgtggcg ggcgcgggcg agggcggtgg gggagccgcc
     5221 ggtgaccgcg acggggacgg cggcggcgtc gagcagctcc aggtcgttgt tgccgtcgcc
     5281 gacggcgagg acgcgggtgg ggtcgaggcc gtgcgccgcg cagaaggaca ggacgccgct
     5341 ccacttgctg gtgcccggcg ggcggacctg gagggtgtgg ccgccgtaga ggaggtcggg
     5401 ggtgacggcg gcgctgcccg cggtcggatc gatcgtggcg gcgacctcgg cgaggcgggc
     5461 gccgtcgccg ccgaccacgg cgatggtgcg gaccggctcg gtacggacca cccgttccag
     5521 gtcgtcgcgg accgcccagg cgcggttgcg ggcgaggtgc ccagggtgtg tgtcgggacg
     5581 cgggccgttg atcatctcca cgccggggcg ctccacgaac aggctggggc tgaggtgggc
     5641 ggcgacgaag gcggcaagca cccgcgaggc gccctcgggt gggaacaccg cctcgtggaa
     5701 gacgctcccg tcacggagat cccggccgat cgccccgtcg agcatcaccg ccggacctgg
     5761 gatcccacca ggctccagca cccaggcggt ggcgcggggc cgccgcccgg tggcgatcag
     5821 caccgggatc ccggcggcct ccagctgatg gaccgccgcg atgacgcgcg ggtggatgca
     5881 ctcgctggcg tcgcataacg tgccgtccag gtcagttacg accagctcga cggcacgttt
     5941 cggcatgggc cgatggtagc gggagctgcc ttcggtggtc ggcgtcgcgc tggggccgga
     6001 ccgtggtggc cgcgcccggc ggcgctcgcg ctccaaccta acctgggtgg accggctccg
     6061 gcgccggggc ttcggctggc ggggccggag ccggggccgg ggccggggcg gctggggttt
     6121 cgcggaggag gccgaggcgg tcgtggaggc ggcgcaggga cgtgggggac caccagttcc
     6181 agcggcccat gagcttcatg gcggcgggga cgaggagggt gcggaccacg gtggcgtcga
     6241 tcacgatggc gagggccatg ccgacgccga tctccttgat ggtgagcagc tcgccgctgg
     6301 cgaagccgag gaacaccgcc acgatcagcg ccgccgccga ggtcacgatc cggccggtgc
     6361 gctgcaggcc gagggcgacg gcgtggtcgt tgtcgccgct ctcgtcccag gtctccttga
     6421 tgcgcgacag caggaagacc tcgtagtcca tggagaggcc gaaggcgaac acgaacacca
     6481 gtaccgggac ggtgatgtcg accgtgccgg tcggctcgaa gccgagcagg cccgacaggt
     6541 ggccctcctg gaacacccag acaagcgcgc cgaagctggc gccgagcgac agcacgttca
     6601 tcaccaccgc cttcagcggc accaccaccg acccggtcat cagccacagc agcagcatcg
     6661 tggccagccc gatcaccccg agcaccagcg gcagccgcgc ggccagcgac tggcggtagt
     6721 cgaccaggaa ggcggccgac ccggtcagct g
//
LOCUS       DASWDC010000200         9120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1634570, whole genome shotgun sequence.
ACCESSION   DASWDC010000200 DASWDC010000000
VERSION     DASWDC010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634570"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..981
                     /locus_tag="VF995_06455"
     CDS             55..981
                     /locus_tag="VF995_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9343241.1"
                     /translation="MAATDVDVFAPSTAPAAIVTGRRRRLRPQRVVLHAFLIATCLIW
                     LAPLAWTVYTSLRPYQYTADHGYFSIGGHYGFDNFTAAWSQADLIKYYGNTLLVTVPA
                     VLLTLLLASFVAFAVARFSWRFNIALLMVFTAGNLLPQQVIITPLYRLFLMIPLPTWM
                     NATGTLYDSYWGIIAIHVAFQTGFCTFVLSNYMRTIPHELTEAALVDGASVWRQFWRI
                     IMPLTRPALAALATLEFIFIYNDFFWAVVLMQSQDKRPITSALLSLQGIDFTNDNLIA
                     AGALLAALPTLVVYFTLQRQFVRGLTLGATKG"
     gene            complement(1067..1747)
                     /locus_tag="VF995_06460"
     CDS             complement(1067..1747)
                     /locus_tag="VF995_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343242.1"
                     /translation="MANRIRGATIAGAATAVLLGTLLAAPAFAAQPPPENFTTDQLCA
                     NVLTKGPGEAPGVTKTVDASGMVTAGQTVHVTVTQNQPETNTKILIAGDCVTLNGTYQ
                     PSLSQSSHPANLQGPWHFSYMVPASAKAGDKFCDRAGVDAQRTGPDNSAHQKSNTVCL
                     TVAAAETTTTTAAPTTTSGATTTSSSAALPFTGSNTWPMAMAGLGLLAAGIVIAMLTS
                     DRRRRSAS"
     gene            1998..2231
                     /locus_tag="VF995_06465"
     CDS             1998..2231
                     /locus_tag="VF995_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX9343243.1"
                     /translation="MTKHEGTKNMVLRLDPALAERLQLVAEVEGRSISDVAREAIAEL
                     VDRRRADDRFIRLLEENLARHQRLLELLRDGEP"
     gene            2690..3115
                     /locus_tag="VF995_06470"
                     /pseudo
     CDS             2690..3115
                     /locus_tag="VF995_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
     gene            complement(3329..3994)
                     /locus_tag="VF995_06475"
     CDS             complement(3329..3994)
                     /locus_tag="VF995_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343244.1"
                     /translation="MSLDVYVGSLTRYYTEGPIAVVEQLARQQRIPYAIVHGNGVDNC
                     PGLARVVRSAVLAWRENLALGLGDRLQASFDWDETLDSPSFNEKPGWDGYGATLLLAA
                     HEEHPELPLPTTVLPDWPDDPAYLAATAEGAGGRFDQLLIPELWLPHPIGCTFRSQDV
                     TGQDVEIGSSVELLTQLRALGEAHPPNGDRPIAATAHEGLRVLIQMAEHSVRHRVPMK
                     LDF"
     gene            4237..5199
                     /gene="deoC"
                     /locus_tag="VF995_06480"
     CDS             4237..5199
                     /gene="deoC"
                     /locus_tag="VF995_06480"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX9343245.1"
                     /translation="MTAMATTPVRLAEAGDGFLRQIELGPILSRFAVDQVGVTERAAA
                     LAKRSIKKTSKVAALRLAVAVMDLTTLEGADTPGKVEALCAKARRPDPADPMVPPVAA
                     ACVYPTMVATAKTALAGSGVRVASVATAFPSGQSSLRVRLADVEDAVAAGADEIDMVI
                     NRGAFLSGRHREVFDEIVAVKGACGAAHLKVILETGELGTYDNVRRASVLAMAAGADF
                     IKTSTGKVQPAATLPVALVMLEAIRDFHERTGRMVGFKPAGGVRSAKQAIQHLILVNE
                     TLGPDWLTPERYRIGASSLLNDCLMQLAKERTGRYQSADYFSKD"
     gene            5209..6690
                     /locus_tag="VF995_06485"
     CDS             5209..6690
                     /locus_tag="VF995_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9343246.1"
                     /translation="MTDQTPVPAASTLETLDLGYAPAPEATDHVRIRDHYGLLIGGKW
                     VDPASGKGFKTVNPATEVVLAEVAEAGEEDVDLAVKAARAAYDKLWSKTSGTQRAKYL
                     FRVARVLQERAREFAVLETIDGGKPIKESRDVDLPLAAAHFFYYAGWADKLDYAFPGR
                     SPRPLGVAAQVIPWNFPLLMAAWKLAPALAAGNTVVLKPAETTPLTALLLGQVLEQAE
                     LPPGVVNILTGAGATGAALVAHPDVDKVAFTGSTEVGKAIQRTLAGSGTRLTLELGGK
                     AANIVFDDAPIDQAIEGIVNGIYFNQGHVCCAGSRLLVQESIAEPLIAKLKRRMATLR
                     VGDPLDKNTDVGAINSRQQLDRIEELVASGEAEGAELYQSPCDLPARGFWFRPTVFTG
                     VAQSHRIAQEEIFGPVLSVLTFRTPAEAVEKANNTPYGLSAGVWTDKGSRILDLASRL
                     RAGVVWANTFNRFDPTSPFGGYKESGFGREGGLHGLAPYLDLS"
     gene            6698..7591
                     /locus_tag="VF995_06490"
     CDS             6698..7591
                     /locus_tag="VF995_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019330101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9343247.1"
                     /translation="MTVADEERLEVRKTYKLFIGGKFPRTESGRSYLVTEGKGGKPLA
                     NACRASRKDVRDAVVAARAAFPTWAGMTAYNRGQILYRIAEMLEGRREQFADEVSRSE
                     GRSRKAAGAEVGKAIDRWVWYAGWADKLAQVFGSSNPVAGPYFNFSVPEPTGVVGVVA
                     PPDSSLLGLVSRLAPVIAGGNTAVVLAAEQRPLPAITLAEVLATSDLPSGVVNLLTGF
                     TAELIGPLAAHMDVNALDLAGLDLALFRDAELAAAESVKRVVAPVNLPAKAWLDDASG
                     QDPYWIAAFLETKTVWHPIGG"
     gene            7848..8507
                     /locus_tag="VF995_06495"
     CDS             7848..8507
                     /locus_tag="VF995_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEX9343248.1"
                     /translation="MVFGLWWVGSDVFNRAGPNIGKGLPRLPTLSTTATTTQRGGTHG
                     RETAAANIQTIQRLGGKVDYGKIDPVTGQRSGIVAVITPAMVRATANDQLGSEPDPSI
                     HPPGWDQLPSRNRARGHLFGRQLGGSGENPANLVTLYQERANTPVMRDYETTVADAVR
                     GGQTVHYEVTPLYASPTDTGPPRAVRLRAQGDHGFRLDVEIANTPQAAVKVNVAPAPT
                     P"
     gene            8542..9111
                     /locus_tag="VF995_06500"
     CDS             8542..9111
                     /locus_tag="VF995_06500"
                     /inference="COORDINATES: protein motif:HMM:NF025919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX9343249.1"
                     /translation="MTDPLPVPLPPSLERLHALVPPPDKPLEAGAPERWPEIEAQLGT
                     TLPEDYKKFTNLYGSGKFDDFLYLFNPFAAPGSGGNLVAEKDAVLAAYTETRSKFPDK
                     FPLPPFPEPGGLLPLGRTDNGNELYWLTEGDPGGWEIALFDGRGSRYELHPPTVTGFL
                     AGLLSGELETTLLPAGFLDQREHSFEPST"
BASE COUNT         1405 a         3330 c         3062 g         1323 t
ORIGIN      
        1 gtacctggtc gcctacttcc gtgccaactt cggtgccagg ggggcgaggt agcgatggcc
       61 gcaaccgacg tcgacgtctt cgccccctcc accgccccgg ccgcgatcgt caccgggcgg
      121 aggcggcgcc tgcggccgca gcgcgtggtg ctgcacgcct tcctgatcgc gacctgcctg
      181 atctggctgg cgccgctggc ctggacggtc tacacctcgc tgcgtcccta ccagtacacc
      241 gccgaccacg gctacttctc gatcggcggg cattacggct tcgacaactt caccgccgcg
      301 tggagccagg ccgacctgat caagtactac ggcaacacgc tgctggtgac ggtgcccgcg
      361 gtgctgctga cgctgctgct ggcctcgttc gtggcgttcg cggtggcccg tttcagctgg
      421 cggttcaaca tcgcgctgct gatggtgttc accgccggga acctgctgcc gcagcaggtg
      481 atcatcacgc cgctgtaccg gctgttcctg atgatcccgc tgccaacctg gatgaacgcc
      541 accggcacgc tgtacgactc ctactggggc atcatcgcca tccacgtggc gttccagacg
      601 ggcttctgca ccttcgtgct gtccaactac atgcgcacca tcccgcatga gctgaccgag
      661 gccgccctgg tcgacggcgc ctcggtgtgg cgccagttct ggcgcatcat catgccgctg
      721 acccgcccgg ccctggccgc gctggccacc ctggagttca tcttcatcta caacgacttc
      781 ttctgggcgg tcgtgctgat gcagagtcag gacaagcgcc cgatcacctc ggcgctgctg
      841 agcctccagg gcatcgactt caccaacgac aacctgatcg ccgccggcgc cctgctcgcc
      901 gccctaccga ccctggttgt ctacttcacc ctccaacgcc agttcgtccg cggcctcacc
      961 ttaggtgcca ccaagggtta ggggttgagg ccggccgtcc gatgacaacg tccccgggag
     1021 cgagttggct cccggggacg ttggacaact gcttgatctg gcgaaactag ctggccgacc
     1081 ggcggcggcg gtcggaggtc agcatggcga tcacgatgcc ggcggcgagc aggccgaggc
     1141 cggccatcgc catcggccag gtgttggaac cggtgaaggg cagggcggcc gagctgctgg
     1201 tggtggtcgc gcccgacgtc gtggtcggtg cggccgtcgt ggtggtggtc tcggctgcgg
     1261 cgaccgtcag gcagacggtg ttgctcttct ggtgggcgga gttgtccggg ccggtccgct
     1321 gcgcgtcgac gcctgcccgg tcgcagaact tgtcgccggc cttggccgac gccgggacca
     1381 tgtagctgaa gtgccacggg ccttggaggt tggccgggtg ggaggactgg ctgaggctcg
     1441 gctggtaggt gccgttgagc gtcacgcagt cgccggcgat caggatcttg gtgttggtct
     1501 ccggctggtt ctgggtgacg gtcacgtgga cggtctggcc ggcggtgacc atgccgctgg
     1561 cgtcgaccgt cttggtcacg cccggcgcct cgccggggcc cttggtgaga acgttggcgc
     1621 agagctggtc ggtggtgaaa ttctccggtg gcggctgcgc cgcgaaggcg ggtgccgcca
     1681 gcagcgtgcc gaggaggact gcggtcgctg cgccagcgat cgtcgccccc ctgatcctgt
     1741 tggccactga tcgctccttc cttggttggt cgatcgccgc tcaagggctt gtatccgcac
     1801 ggggcgggag aataccccac gacggaagac aactcgaaac tcggggcgag tcccgggggg
     1861 aagcggaact aaccctacac ggacatcgct agacaaccac cgcatgccaa acttagccga
     1921 atttgttacc ggatcgaaat ccggcgtggt tgacccgagt tgtcgctatc aaggtgtcgt
     1981 gatagtgtga tatcactatg acgaagcacg agggcaccaa gaacatggtc ctgcggctcg
     2041 atccggccct ggccgagcgg ctgcagctgg tggcggaggt cgagggccgc tccatctccg
     2101 acgtggcgcg ggaagcgatc gccgaactgg tcgatcgccg ccgcgccgac gaccgcttca
     2161 tccgcctcct ggaagagaac ctggccagac accagcgcct gctggagctg ctgcgggacg
     2221 gtgagccgtg atccacctcg acctcgccga cgttgtcgtg atcgcggccc gggtgctcgg
     2281 cgccgagccg gccgacgtgc tcgccgtcgc cgacctcgac ggtgtggagg cggccctggc
     2341 caaggcccgc cgctgcgaca gcccaccccg ggccgccgcg acgctgctgc acggcctggc
     2401 gttgcgccag ccgttcgggc ccgggagtgg ggaggtggcg ctgctcggcg ccctccagct
     2461 gctggcgctc aacggccgcg aggtccgcga cctcggtgcc cccgacgtcg cgcgcaagct
     2521 gctcgaccgg accgcgcagg gcacggccga cgtggacgag ctctgcgcct ggctcgtcgc
     2581 tggaggcagc ggcatcaccg agatcaagga gcggccgatc gctgaggcga tccggcgggt
     2641 ccgcgagctg cgcccacggc ggcagaagga caaggagagc gaggaagaga tgttcgagcg
     2701 gttcaccagt cgcgctcgcc aggtggtggt gctggcccag gacgaggcca ggcgcctcaa
     2761 tcacaactac atcggcaccg agcacatcct gctcggcctg atccacgagg gcgaaggcgt
     2821 cgccgccaag gcgctggagt cgctcgggat ctcgctggaa gtggtccgca cccaggtcga
     2881 ggagatcatc ggccagggcc agagcgcccc gaccgggcac atccccttca ccccacgagc
     2941 caagaaggtc ctgcagctgg cgctgcgtga ggcgctcagc ctgcaccaca actacatcgg
     3001 caccgagcac gtcctgctgg gcctcgtccg ggaaggcgag ggcgtcgccg cccagatcct
     3061 ggtgaagctc ggcgccgacc ttccccgcgt ccgccagcag gttgtccagc tcctcggcag
     3121 cgggcagttc gtggaagcgt tcagcgagga gcaggccaag cagcgcgaac gggagttcac
     3181 cgccaacctc gcccgggtct tcgacgagaa ccgccatctg cgcgccgagg tcgaacggct
     3241 ccgggggctg ctgcgccagc acggcatcaa gccggacagc ggcgaatcga agaccgcctg
     3301 acgcgcggcc agatacgtca gagaggactt agaagtcgag cttcatcggg acccggtggc
     3361 gcaccgagtg ctcggccatc tggatgagga cccgcaggcc ctcgtgagcg gtggccgcga
     3421 tcgggcggtc accgttgggc gggtgggcct cgccgagggc gcggagctgg gtgagcagct
     3481 cgacactgga gccgatctcg acgtcctggc cggtgacgtc ctgggagcgg aaggtgcagc
     3541 cgatcgggtg ggggagccac agctcgggga tcagcagctg gtcgaaacgg ccgccggcac
     3601 cctcggcggt ggccgccagg taggccgggt cgtcgggcca gtcgggcagc acggtggtcg
     3661 gcagcggcag ctccgggtgc tcctcatggg cggccagcag cagggtcgcg ccgtagccgt
     3721 cccagcccgg cttctcgttg aagctggggc tgtcaagcgt ctcgtcccag tcgaacgagg
     3781 cctgcagccg gtcgccgagc ccgagcgcga ggttctcgcg ccaggccagc acggcgctgc
     3841 gcaccacccg ggcgagaccc gggcagttgt ccaccccgtt gccgtggacg atcgcgtagg
     3901 ggatccgctg ctgccgggcg agctgctcga ccacggcgat cggaccctcg gtgtagtacc
     3961 gggtcagcga gccgacgtag acatcaaggc tcatcgcatc ccctcgctcc ccggattaat
     4021 ctgtctgaac tgattatttc gaacgatacc cccgatcacg tcgcacttac cagcaacgat
     4081 ggatcgtgat tcggttgtta ccgtccagca tcgtcctcaa gggtcacagg atggtgacac
     4141 gatcgtcgcg ttcccgagtg ggttctccag taggtcgccg ggaaaacgcc aactgccaag
     4201 tcgctacaat cgccgctgga ccaacgaggc tgggaggtga cggccatggc gaccacaccg
     4261 gtgcgactcg ccgaggccgg cgacggcttc ctacgccaga tcgagctggg gcccatcctg
     4321 tcccgcttcg cggtcgacca ggtcggcgtc acggagcggg ccgccgccct cgcgaaacgg
     4381 agcatcaaga agacctccaa ggtggccgca ctgcggctcg ccgtcgcggt catggacctg
     4441 accaccctgg agggcgccga cacccccggc aaggtcgagg ccctctgcgc caaggcccgc
     4501 cggcccgacc cggccgaccc gatggtcccg ccggtggccg ccgcctgcgt ctatccgacg
     4561 atggtcgcca ccgccaagac cgccctcgcc ggctccggcg tccgggtcgc ctcggtggcg
     4621 accgccttcc caagcggcca gtcgtcgctg cgggtgcgcc tcgccgacgt ggaagacgcg
     4681 gtcgccgccg gcgccgacga gatcgacatg gtgatcaacc gcggcgcctt cctgtccggc
     4741 cgccaccgcg aggtgttcga cgagatcgtc gccgtcaagg gggcctgcgg cgccgcgcac
     4801 ctgaaggtga tcctggagac cggggagctc ggcacctacg acaacgtccg ccgcgccagc
     4861 gtgctcgcca tggcggccgg cgccgacttc atcaagacct ccaccggcaa ggtccagccc
     4921 gccgccaccc tgccggtcgc gctggtgatg ctggaggcga tccgcgactt ccacgagcgc
     4981 accggccgca tggtcggctt caagccagcc ggcggcgtcc gcagcgccaa gcaggccatc
     5041 cagcacctga tcctggtcaa cgagaccctc gggcctgact ggctcacccc cgagcgctac
     5101 cgcatcggcg cctccagcct gctcaacgac tgcctcatgc agctcgccaa ggaacgcacc
     5161 ggccgctacc agtccgccga ctacttctcc aaggactgag cgaagcagat gactgatcag
     5221 acgcccgtcc ccgccgcctc caccctggag accctggacc tcggctacgc gcccgcgccg
     5281 gaggcgaccg accacgtcag gatccgcgac cactacggcc tgctgatcgg cggcaaatgg
     5341 gtggacccgg cgtctggcaa gggcttcaag accgtcaacc ccgctaccga ggtggtgctc
     5401 gccgaggtcg ccgaggcggg ggaggaggac gtcgacctcg ccgtcaaggc cgcccgcgcc
     5461 gcctacgaca agctctggtc caagacctcc gggacgcagc gcgccaagta cctgttccgc
     5521 gtcgcccgtg tcctgcagga gcgcgcccgc gagttcgcgg tgctggagac gatcgacggc
     5581 ggcaagccga tcaaggagtc ccgcgacgtc gacctgccgc tggccgccgc gcacttcttc
     5641 tattacgccg gctgggccga caagctcgac tacgccttcc cgggccgctc gccccggccg
     5701 ctcggggtcg ccgcgcaggt gatcccctgg aacttcccgc tgctgatggc cgcctggaag
     5761 ctcgccccgg cgctggccgc cggcaacacg gtggtcctca agccggccga gaccaccccg
     5821 ctcacggctc tgctcctcgg ccaggtcctg gagcaggccg agctgccgcc cggtgtggtc
     5881 aacatcctca ccggcgcggg cgcgaccggc gcggcgctgg tcgcccatcc cgacgtggac
     5941 aaggtcgcct tcaccggctc caccgaggtc ggcaaggcga tccagcgcac cctcgccggg
     6001 tccggcacgc gcctcaccct ggagctgggc ggcaaggccg ccaacatcgt cttcgacgac
     6061 gccccgatcg accaggcgat cgaggggatc gtcaacggca tctacttcaa ccagggccac
     6121 gtctgctgcg ccggctcccg cctgctggtc caggagtcca tcgccgagcc gctgatcgcc
     6181 aagctcaagc gccgcatggc caccctgcgg gtcggcgacc cgctcgacaa gaacaccgac
     6241 gtcggcgcga tcaactcccg ccagcagctc gacaggatcg aggagctggt cgcaagcggc
     6301 gaggcggagg gcgccgagct gtaccagtcc ccgtgcgacc tgcccgcccg cggcttctgg
     6361 ttccgcccca cggtcttcac cggcgtcgcc cagtcccacc gcatcgccca ggaggagatc
     6421 ttcggcccgg tcctgtcggt gctgaccttc cgcacccccg ccgaagccgt cgagaaagcc
     6481 aacaacaccc cctacggcct ctccgccggc gtctggaccg acaagggcag ccgcatcctc
     6541 gacctcgcca gccgcctccg cgccggcgtc gtctgggcca acaccttcaa ccgcttcgac
     6601 cccaccagcc ccttcggcgg ctacaaagaa tccggcttcg gccgcgaggg cggcctccac
     6661 ggcctcgctc cctatctgga tctgtcatga ggaggcactg accgtggctg acgaggagcg
     6721 gctcgaggtg cgcaagacct acaagctgtt catcgggggg aagttcccgc ggaccgagtc
     6781 ggggcggtcg tatctggtga ctgaaggcaa gggcgggaag ccgctggcca acgcgtgccg
     6841 agcctcgcgg aaggacgtgc gggacgcggt ggtggcggcg cgggcggcgt ttcccacctg
     6901 ggcggggatg accgcctaca accggggcca gatcctctac cggatcgcgg agatgctgga
     6961 gggccggcgt gagcagttcg ccgacgaggt cagccgctcc gagggccgca gccgcaaggc
     7021 cgccggcgcg gaggtcggca aggcgatcga ccggtgggtc tggtacgccg ggtgggcgga
     7081 caagctcgcc caggtgttcg ggtcttccaa cccggtcgct ggaccgtact tcaacttctc
     7141 ggtccccgag ccgaccggcg tggtcggggt ggtcgcccca ccggactcct cgctgctcgg
     7201 gctggtgtcg cggctcgcgc cggtgatcgc cggcggcaac accgccgtgg tgctcgccgc
     7261 cgagcagcgg cccctgccgg cgatcacgct ggcggaggtg ctggccacca gcgacctccc
     7321 cagcggggtg gtgaacctgc tcaccggctt caccgccgag ctgatcgggc cgctggccgc
     7381 ccacatggac gtcaacgcgc tcgacctggc cggcctcgac ctggccctgt tccgcgacgc
     7441 ggagctggcc gccgccgaga gcgtcaagcg ggtggtcgcg cccgtcaacc tccccgccaa
     7501 ggcctggctc gacgacgcca gcggccagga tccctactgg atcgccgcgt tcctggagac
     7561 caagaccgtc tggcacccca tcggtgggtg agcgggcgtg tgcggccggg cggatatgct
     7621 cacgcttcgc tgggtaggaa cggcgtgact gttccgccct gccgccggcc gagcgtgcac
     7681 gcgagcgatg cgacgcggtc gacgagcggc cggcggagac gaccgcatgg gggcccgacc
     7741 gccgcatggc cagaacccga agcaggagca gaccccggcg aagaagccga agtccgcggc
     7801 gccggagccg cggctcgaac ctgggcgcgg ccctggccgt cctgctggtg gtgttcgggc
     7861 tctggtgggt cggatccgac gtcttcaacc gcgctgggcc gaacatcggc aagggcctcc
     7921 ccaggctccc gaccctcagc acaacagcaa cgaccaccca gcgcggcggg acccacggcc
     7981 gcgagacggc cgccgccaac atccagacca tccaacgcct cggcggcaag gtcgactacg
     8041 gcaagatcga cccggtcacc ggccagcgca gcggcatcgt cgccgtcatc accccggcga
     8101 tggtccgggc gaccgcgaac gaccagctcg gctccgaacc cgacccgtcg atccacccgc
     8161 ccggctggga ccagctcccg tcccgcaacc gcgcccgcgg gcacctgttc ggccgccagc
     8221 tcggcggctc cggcgagaac ccggccaacc tcgtcaccct ctaccaggag cgcgccaaca
     8281 cccccgtcat gcgcgactac gaaacgacgg tcgccgacgc ggtccgcggc ggccagacgg
     8341 tccactacga ggtcacgccg ctctacgcca gccccaccga caccggcccg ccgcgggcgg
     8401 ttcgcctgcg cgcccagggc gaccacggct tccgcctcga cgtggagatc gccaacaccc
     8461 cgcaggccgc cgtcaaggtc aacgtcgccc cggccccgac cccttgacgc gacgcgaggc
     8521 gagctgagct gaggtgaccc agtgaccgat ccgctgccgg tgccgctgcc gccctcgctg
     8581 gagcggctcc acgccctggt cccaccaccg gacaagccgc tggaggccgg cgcccccgag
     8641 cgctggcccg agatcgaggc tcagctcggc accaccctgc ccgaggacta caagaagttc
     8701 accaacctct acggctcggg caaattcgac gacttcctct acctcttcaa cccgttcgcc
     8761 gcgcccggca gcggcggcaa cctcgtcgcc gaaaaagacg cggtcctcgc cgcctacacc
     8821 gagacccgga gcaaattccc cgacaaattc ccgctcccgc cgttccccga gcccggtggg
     8881 ctgctgccgc tcggccgcac cgacaacggc aacgagctgt actggctcac cgaaggcgac
     8941 ccgggcggct gggagatcgc cctcttcgac ggccgcggca gccgctacga gctgcacccg
     9001 cccaccgtca cgggattcct ggccgggctc ctctccggcg aactggagac caccctgctc
     9061 ccggccgggt tcctcgacca gcgcgagcac agcttcgagc cgtccacctg accccaggcg
//
LOCUS       DASWDC010000201         9253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_300517, whole genome shotgun sequence.
ACCESSION   DASWDC010000201 DASWDC010000000
VERSION     DASWDC010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300517"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VF995_06505"
     CDS             complement(<1..191)
                     /locus_tag="VF995_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX9343250.1"
                     /translation="MTLEFVKAHGTGNDFVVIEDLDDRYGSTLNAAFVRQVCDRHLGI
                     GADGLIRIAPGTAAPYFMD"
     gene            complement(253..1224)
                     /gene="miaA"
                     /locus_tag="VF995_06510"
     CDS             complement(253..1224)
                     /gene="miaA"
                     /locus_tag="VF995_06510"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX9343251.1"
                     /translation="MTVTMPLPGPVLALVGPTASGKTALALALAARLDAEVEVVSADA
                     MLVYREMDIGTAKPTPAERALVPHHLVDLVDPGEEFSVARFQPLARAAIAEVLGRGRV
                     PLLVGGSGLYFHAVVDDFVFPPTDPAVRARMEVEAATVGLPALYQRLQAQDPAAAARI
                     QPANLRRTVRALEVIELTGQPFSSFRIPGAIDEPASRYRLTVLGLDPGVDCLRERVAA
                     RVEAMVAAGLVEEVRRLARQPLSRTARQALGYKELLDAMEAAAAEGEPLEGAALAAAL
                     AAVVARTRAYARRQLAWFRRDRRIEWSTLPPGPELAAWALEQYETEL"
     gene            complement(1239..2759)
                     /gene="miaB"
                     /locus_tag="VF995_06515"
     CDS             complement(1239..2759)
                     /gene="miaB"
                     /locus_tag="VF995_06515"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014986797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX9343252.1"
                     /translation="MSLELGELGPERQRYLVRTFGCQMNEHDSERIAGLLEAEGMRPA
                     SGLDDADLVVLNTCAVRENADNKLYGTLGHLATAKASRGLMIAVGGCLAQKDQGTVLR
                     KAPWVDVVFGTHNIGSLPVLLRRAREQGRAQAEFFDEMQRFPSALPVRRDSDWSAWVA
                     ISVGCNNTCTFCIVPALRGKERSRRVGDVVAEVQALARAGVVEVTLLGQNVNSYGTDL
                     PGHRQLFGDLLRALDQVEGLERLRFTSPNPKDFRDDVVDAMAACRPLCAHVHFPLQSG
                     SDRVLRLMRRGYRRRRYLEILAKLRAAIPGVAFSTDIIVGFPGETEDDFAQTLEVVEA
                     ARFDSAFTFQYSPRPGTEAATMPGPVPPEVVRERFGRLLALQEGISLERNRALVGTEV
                     ELLVERASSKTDPTRMSGRTRGNKLCHFPAPASTPSGETVAPGDFVTVRVEQAAPHHL
                     LAGPVLAHRPRRVPVTTTEEAAACGIAPPSRPLPAGAVPLPLLTSTATSASATAPH"
     gene            complement(2845..4431)
                     /gene="rny"
                     /locus_tag="VF995_06520"
     CDS             complement(2845..4431)
                     /gene="rny"
                     /locus_tag="VF995_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX9343253.1"
                     /translation="MVEPGILFGILGAVVVGAGVGFAVGTTLERKHAANLLGSAEDQA
                     KAVLEDARRQAETSRREAAVEARDAALQLRQEAEGDALKLRAEAEAEARSRFTEVQRR
                     EARLLGREEALEDKAGLIDRREASLHEREESAEALRRDLEAAAEEQRRRLEQIAAMTA
                     SEAKLLLIKQIEEEAKRDAMGLVRDLEQQAREEADRRARKIVTLAIQRIASEQTSEST
                     VAVLPLPSDDMKGRIIGREGRNIRAYEAVTGVNLIIDDTPEAVLLSCFDPVRREVGRL
                     TLEKLVSDGRIHPARIEETYERAKVEVESEVRRAGEDACLEVSITDLHPELIRVLGRL
                     KYRTSYGQNVLRHLVETAHLAGIMAAELGMDVQLAKRCGFLHDIGKALTHEVEGSHAI
                     IGAELARRLKEAPEVVHAIEAHHGEVEQRTVEAVVCQAADAISGGRPGARRETLESYV
                     KRLGRLEEISASYPGVDKVFAMQAGREVRIMVRPEDCDDVAAQVLARDVAKRIEQELQ
                     YPGQIKITVVRESRSIEYAK"
     gene            complement(4792..5223)
                     /locus_tag="VF995_06525"
     CDS             complement(4792..5223)
                     /locus_tag="VF995_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX9343254.1"
                     /translation="MRLLTVRARSQAELEQRLGQRGFEAGAVAAALERLMAVGLVDDD
                     AFAAALAESRAARGMDAAAVALELRERGVDPALAARKAGEAMPAEDRVERCRQVAEAR
                     LAQLGGLSPATQFRRLAAYLARRGYATEVIHSVVGDLLGFE"
     gene            complement(5348..6508)
                     /gene="recA"
                     /locus_tag="VF995_06530"
     CDS             complement(5348..6508)
                     /gene="recA"
                     /locus_tag="VF995_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005311134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX9343255.1"
                     /translation="MATTYTARNGSVRGGTVEREKALEVALLQIEKQFGKGAVMRMGI
                     DGPAAPIEAIPTGSLALDLALGIGGVPRGRVVEIYGPESAGKTSVALHIIAEAQRLGG
                     IAAFIDAEHALDVTYARNLGVNTDELLISQPDTGEQALEIADMLIRSGAIDVVVIDSV
                     AALVPRAEIEGEMGDSHVGLQARLMSQALRKLAGTISKSKTTAIFINQLREKVGVLFG
                     SPETTSGGRALKFYASVRLDVRRIESLKDGPDVVGNRTRVKVVKNKVAPPFKQAEFDI
                     MYGQGISKEGSLLDVGVDVGLIKKSGAWFTYEGDQLGQGRENARNFLRDNPELAREIE
                     GKIKAQLGIGMPPEPESLSGDENVAMVLTEAPVPAVPKPSSDGKGKRGSASR"
     gene            complement(6741..7382)
                     /gene="thpR"
                     /locus_tag="VF995_06535"
     CDS             complement(6741..7382)
                     /gene="thpR"
                     /locus_tag="VF995_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX9343256.1"
                     /translation="MSRASSSFSFSDPDLLTGRLFFAVPVPGPTRAPLEALLPELGRA
                     LPGARLPVASGWHLTLAFLGNVRAEEAAAVVGVGETAAAAAQPANLQLHGAGAFPNPR
                     RARVLWAGVASEFETLVTLAAALAAACREAGLRFEDRDLVPHLTLARLPQPAPLPEPV
                     IELVTAASAQSPGWRARELCCYRSIISRTGARYQVVRSFPLGGEVARQPTEQP"
     gene            complement(7396..8646)
                     /locus_tag="VF995_06540"
     CDS             complement(7396..8646)
                     /locus_tag="VF995_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX9343257.1"
                     /translation="MRAEIVGVGTEILLGQISNSNAQWISERLGEIGVDVVYHQAVGD
                     NEDRIREAFSLALSRADVVIATGGLGPTQDDITREGLAAALGVPLIRRPEIEDFLRQK
                     FQRLGRAMPEMNLRQADVPEGARAIDNPRGTAPGLIIEVGGKPLYAVPGVPREMEGML
                     ERVVLPDLAARAGEGRAIVSRTLRTAGLGESALAERLTPLWESMGEEAGVTLAYLASP
                     GEVRVRLTTVGATREQALATIAPVEEAVRKELGDLVYGLDDETLEAAVGRLLRAAGQT
                     LASAESLTGGLAGGRITAAAGASDYYLGGVVVYATDAKAELLGVDAALLAEHGPVSEP
                     VAGAMAEGVRRLFGAGVGIATTGVAGPTEQDNKPVGSFCLGVADPDGTVTRSTRAPGD
                     RAQVRAWATTTALDLVRRRLEGKA"
     gene            complement(8643..>9253)
                     /gene="pgsA"
                     /locus_tag="VF995_06545"
     CDS             complement(8643..>9253)
                     /gene="pgsA"
                     /locus_tag="VF995_06545"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX9343258.1"
                     /translation="PREPVSEREAAGAAGSLSPFGFGWPNVISALRILLVPAIVWLIL
                     AGDGDRDAAAVLFVLGGATDGVDGYIARRYGSVSRTGQWLDPLADKVLVAAPIITLAS
                     MGEFPLWALAIFLVREAAISVLRVVLGLRGIALPASRSAKLKTTLQLTAITLYVLPLG
                     PSAHGARLAVLVVAIALTAATGLEYLVRGTRWLRAPAAPAPR"
BASE COUNT         1270 a         3269 c         3365 g         1349 t
ORIGIN      
        1 tagtccatga aatatggcgc cgccgttccc ggtgcgatcc ggatgagccc gtccgcgccg
       61 atcccaaggt gccggtcgca gacctgccgc acgaacgcgg cattgagcgt ggagccgtag
      121 cggtcgtcca ggtcctcgat caccacgaag tcgttgccgg ttccgtgcgc cttgacgaac
      181 tccaaggtca tgcacgctcc gttgtcgtca tgggtgtcgt catgcgccgc accggtgcgc
      241 tcaccctccc gttcagagct ccgtttcgta ctgttccagc gcccaggccg caagctccgg
      301 cccgggggga agggtactcc actcgatccg gcggtcccgc cggaaccagg ccagctggcg
      361 gcgggcatag gcgcgggtgc gggcgaccac cgccgccagc gccgccgcca gcgccgcgcc
      421 ctccagcggc tccccctccg ctgccgccgc ctccatcgcg tcgagcagtt ccttgtagcc
      481 gagggcctgg cgggcggtgc gcgacagcgg ctggcgggcg agccgccgca cctcctcgac
      541 caggccagcg gcgaccatcg cctcgacccg cgcggccacc cgctcccgca ggcagtccac
      601 accggggtcc aggccgagga cggtcagccg gtaccgcgac gccggctcgt cgatcgcccc
      661 cgggatccgg aaggaggaga acggctgccc ggtcagctcg atcacctcca gcgcccgcac
      721 ggtgcggcgc aggttggccg gctggatgcg tgccgccgcc gccgggtcct gggcctggag
      781 ccgttggtag agggcgggca ggccgaccgt tgccgcctcg acctccatgc gggcgcgcac
      841 cgccgggtcg gtcggcggga agacaaaatc gtcgacgacc gcgtggaagt acaggccgga
      901 tccgcccacc agcagcggca cccggccgcg gccgagcacc tccgcgatcg ccgcccgggc
      961 cagcggctgg aagcgggcca ccgagaactc ctcgcccgga tcgaccaggt cgaccaggtg
     1021 atgcgggacc agggcacgct ccgccggggt cggtttggcg gtgccgatgt ccatctcgcg
     1081 gtagaccagc atcgcgtcgg cgctgaccac ctcgacctcg gcgtcaaggc gcgcggcgag
     1141 cgccagggcg agtgcggtct tgccggaggc ggtcgggccg accagtgcca gcaccgggcc
     1201 gggcaggggc atggtcacgg tcacggcggc gccgccgctc agtgcggcgc cgtcgcggaa
     1261 gcggaagtgg cggtggaggt gagcagcggc agcggcaccg caccagcggg gagcggccgg
     1321 gatggcgggg cgatcccgca ggccgccgcc tcctccgtgg tcgtgacggg gacgcgccgt
     1381 ggtcggtggg cgagcaccgg cccggcgagc aggtggtgcg gggcggcctg ctcgacccgg
     1441 acggtgacga agtcgccggg cgcgaccgtt tctccgctgg gggtggaggc cggggctggg
     1501 aagtggcaga gcttgttgcc gcgggtccgg ccggacatcc gggtcgggtc ggtcttggag
     1561 ctggcccgct cgaccagcag ctcgacctca gtgccgacca gggcgcggtt gcgttccagg
     1621 ctgatgccct cctggagggc gagcaggcgg ccgaagcgct cccggaccac ctcgggcggg
     1681 accgggccgg gcatagtggc ggcctcggtg ccgggccggg gcgagtactg gaaggtgaag
     1741 gcggagtcga agcgggccgc ctcgacgacc tccaaggtct gagcgaagtc gtcctcggtc
     1801 tcgccgggga acccgacgat gatgtcggtc gagaacgcca cgcccgggat cgccgcacgg
     1861 agcttggcga ggatctccag gtagcggcgg cgccggtagc cgcgccgcat cagccgcagg
     1921 acccggtcgg agccggactg gagcgggaag tggacgtgcg cgcagagtgg ccggcaggcg
     1981 gccatggcgt cgaccacgtc gtcgcggaag tccttggggt tcgggctggt gaaacgcagc
     2041 cgctccaacc cctcgacctg gtcgagggcg cgcagcaggt cgccgaacag ctgccggtgg
     2101 ccgggcaggt cggtgccgta ggagttgacg ttctggccga gcagggtcac ctcgaccacc
     2161 ccggcacggg cgagcgcctg gacctcggcg accacgtcgc cgacgcggcg gctgcgttcc
     2221 ttgccgcgca gggccggcac gatgcagaag gtgcaggtgt tgttgcagcc gacgctgatc
     2281 gcgacccacg ccgaccagtc gctgtcgcgc cgcaccggca gcgccgaggg gaagcgctgc
     2341 atctcgtcga agaactcggc ctgggcgcgg ccctgctccc gggcgcggcg cagcagcacc
     2401 ggcaggctgc cgatgttgtg ggtgccgaag accacgtcga cccagggggc cttgcgcagg
     2461 accgtgccct ggtccttctg ggcgaggcag ccgcccaccg cgatcatcag cccgcggctg
     2521 gccttggcgg tggcgaggtg gccgagggtg ccgtagagct tgttgtcggc gttctcccgc
     2581 accgcgcagg tgttgagcac gaccaggtcg gcgtcgtcga gcccgctggc cggacgcatg
     2641 ccctcggctt cgagcaggcc ggcgatccgc tccgagtcgt gctcgttcat ctggcacccg
     2701 aaggtgcgca ccaggtaccg ctggcgctcc ggtcccagtt ctcccagttc caggctcatc
     2761 aggcggccat gctaccgcgc ccacgccgcg cccacgccgc gcccacgctg ccgccaggcc
     2821 tccccgcctc cgctgcctgt gtggttactt cgcgtattcg atgctgcggc tttcgcgcac
     2881 cacggtgatc ttgatctggc ctgggtactg gagttcctgc tcgatgcgct tggcgacgtc
     2941 gcgggcgagg acctgggcgg cgacgtcgtc gcagtcctcg gggcggacca tgatgcggac
     3001 ctcgcggccg gcctgcatgg cgaagacctt gtcgacgccg gggtagctgg ccgagatctc
     3061 ctccaggcgg ccgaggcgct tgacgtagga ctccagggtc tcgcggcggg cgccggggcg
     3121 gccgccggag atggcgtcgg ctgcctggca gacgaccgcc tcgacggtcc gctgctcgac
     3181 ctcgccgtgg tgggcctcga tggcgtggac gacctcgggt gcctccttga ggcggcgggc
     3241 cagctccgcg ccgatgatgg cgtgggaccc ctcgacctca tgggtgagcg ccttgccgat
     3301 gtcgtgcagg aacccgcagc gtttggcgag ctggacgtcc atgcccagct cggcggccat
     3361 gatcccggcg aggtgggcgg tctcgaccag gtgccggagc acgttctggc cgtaggaggt
     3421 gcggtacttg aggcggccga gcacccggat cagctcgggg tgcaggtcgg tgatgctgac
     3481 ctccaggcag gcgtcctcgc cagcgcggcg gacctcgctt tcaacctcga ccttggcgcg
     3541 ttcgtaggtt tcctcgatgc gggcggggtg gatgcggccg tcggagacga gcttctccag
     3601 ggtgaggcgg ccgacctcgc gccgcaccgg gtcgaagcag gacagcagca cggcctcggg
     3661 ggtgtcgtcg atgatcaggt tgacgccggt gaccgcctcg taggcgcgga tgttgcggcc
     3721 ttcgcggccg atgatccggc ccttcatgtc gtcggagggc agcggcagga ccgccacggt
     3781 ggactccgag gtctgctccg aggcgatccg ctggatcgcc agggtgacga tcttgcgggc
     3841 gcgccggtcg gcctcttcgc gcgcctgctg ctcgaggtcg cggaccaggc ccatggcgtc
     3901 ccgcttggcc tcctcctcga tctgcttgat cagcaggagc ttggcctcgc tggcggtcat
     3961 ggcggcgatc tgctccagcc ggcgtcgctg ctcctcggcg gcggcttcca ggtcgcggcg
     4021 cagcgcctca gcggattcct cacgttcgtg caggctcgcc tcgcgccggt cgatcaagcc
     4081 cgccttgtcc tcaagcgcct cctcccggcc gagcagccgt gcctcgcggc gctgtacctc
     4141 ggtgaagcgg ctcctggcct cggcttctgc ctcggcgcgg agtttgagtg cgtcgccctc
     4201 cgcctcctgg cggagctgga gggcggcgtc gcgcgcttcg accgctgctt cccgtctgga
     4261 ggtctccgcc tgccgtcgtg cgtcctcaag aactgctttg gcctgatcct cggccgagcc
     4321 gagcaggttg gccgcgtgct tgcgctccag ggtcgtgccc acggcgaagc caacgcccgc
     4381 gccgacgacg accgctccca aaatcccaaa gagaatgccg ggttccacca cggcctcctt
     4441 tgcgctccat ccggccggga catccagccg gtttcaggcc gcttcggccg tggccgcatc
     4501 cattggccat agaacggcaa acgcgccgag cggccgcccg gcgctactga gacacccttg
     4561 attacgacgc gaaggccgcg tccgacagga cgatcgtcga tgagccagag gcgacgcctc
     4621 tgcccccaaa gcagctagtc cggtggacgg gtcgacgtag caaggtttac gtaatcatag
     4681 gttgagctcg gcaagaccgg gcgaacccag ggtaggccgg caaccctgcg tggtcaagga
     4741 acagcaggtc agaggcttgt caaacccctg gatcccgtca ggacgggtga atcactcgaa
     4801 tccgagcaga tcgcctacta cagagtggat aacctcggtt gcgtagcccc gtcgggccag
     4861 atatgccgca aggcggcgga attgcgtcgc ggggctgagc ccgccgagct gggccagccg
     4921 cgcctcggcg acctgccggc agcgctcgac ccgatcctcg gcgggcatcg cctcgccggc
     4981 tttgcgggcc gcgagcgccg gatcgacgcc gcgctcgcgc agttcgagcg cgaccgcggc
     5041 ggcgtccatc ccgcgtgccg cccggctttc ggccaaggcg gcggcgaagg cgtcgtcgtc
     5101 gaccagcccg accgccatca gccgctccaa cgccgccgcc accgctccgg cctcgaagcc
     5161 gcgctggccg agccgctgct ccaattccgc ctggctccgc gctcgcacgg tcagcagccg
     5221 caacgcggcg gcgtaggccg cgtcccccga cgcggcctgg tcgccgtcca agcctgcccc
     5281 ggcccgctgc tgcactggcc gggacctgac tacccgccgt tccgccacca tgtcacccag
     5341 tcgacggtca gcgcgaggcc gagccacgct tgcccttccc gtccgagctc ggcttgggga
     5401 ccgcgggcac gggcgcctca gtcagcacca tggcgacgtt ctcgtctccg ctcaggctct
     5461 ccggctccgg tggcatcccg atccccagtt gggccttgat cttgccctcg atctctcgcg
     5521 ccagctcagg gttgtcccgc aggaagttgc gggcgttctc ccgcccctgc ccgagctggt
     5581 caccttcgta ggtgaaccag gctccggact tcttgatcag cccgacgtcc acgccgacgt
     5641 cgagcaggct gccctccttg gagataccct ggccatacat gatgtcgaac tccgcctgct
     5701 tgaacggcgg cgcgactttg ttcttcacga ccttcacgcg ggtccggttc ccgaccacgt
     5761 ccgggccgtc cttcagcgac tcgattcggc gcacgtcaag ccggaccgag gcgtagaact
     5821 tcagcgcgcg gccgccagac gtcgtctcgg gagacccgaa gaggacgccg accttctcac
     5881 gcaactggtt gatgaagatc gcggtggtct tcgacttgga gatcgtgccg gcgagcttgc
     5941 ggagggcttg gctcatgagg cgggcctgga ggccgacgtg ggagtcgccc atttcgccct
     6001 cgatctcagc gcgggggacc agcgcggcga cggagtcaat cacgacgacg tcgatggcgc
     6061 cggagcggat cagcatgtcg gcgatctcaa gggcctgctc gccggtgtcg ggctgggaga
     6121 tcagcagctc gtcggtgttg acgccgaggt tgcgggcgta ggtgacgtcg agggcgtgct
     6181 cggcgtcgat gaaggcggcg atgccgccga gccgttgcgc ctcggcgatg atgtgcaacg
     6241 ccacggaggt tttaccggct gattccgggc cgtagatttc gaccacgcgg ccacggggga
     6301 cgccaccgat gccgagagcg aggtcgaggg cgagactgcc ggtcgggatc gcctcgatgg
     6361 gggcggcggg gccgtcgatg cccatgcgca tgaccgcgcc cttgccgaat tgtttctcga
     6421 tctggagcag ggcgacttcc agtgcttttt cgcgctccac ggtccctccc cggacgctgc
     6481 cgttccgagc agtataggtc gtggccatga cattcctttc gtggtctgcg gcggttgctg
     6541 gccggctaaa ggtcggggca gtgggaggct ccgcccagcg ggtgggagca ccctactacg
     6601 aacgtctgtt cgccacaatg atcatagacc gaactgtgga caaccaattc cccgggggga
     6661 tcaagacgtt cgggtggcac ggtcccgcgg ccggcaggac cacggccgat cccatggtgg
     6721 ccgcggggcc ccccgatggg tcagggctgc tcggtgggct gccgcgcgac ctcgccgccg
     6781 agcgggaagc tgcggaccac ctggtagcgg gcgccggtgc ggctgatgat gctgcggtag
     6841 cagcacagct cgcgggcccg ccagccgggg ctctgggcgc tggccgcggt gaccagctcg
     6901 atcaccggct cgggcagcgg tgcgggctgc ggcagccgag ccaaggtcag gtgcgggacc
     6961 aggtcgcgat cctcgaagcg caggccggcc tcgcggcagg cggcggccag cgccgccgcg
     7021 agggtgacca gcgtctcgaa ctcgctggcg acaccagccc acagcacccg agcccggcgc
     7081 gggttcggga aggccccagc gccgtggagc tggaggttgg cgggttgggc ggcggccgcg
     7141 gcggtctcgc cgacgccgac gacggcggcg gcctcctcgg cgcggacgtt gccgaggaac
     7201 gccagggtca gatgccagcc gctcgccacc ggcaggcggg cgccggggag ggcccggccg
     7261 agctccggca gcagcgcctc cagcggcgcc cgggtcggtc ctggcaccgg cacggcaaag
     7321 aacaacctgc cggtgagcag gtccgggtcg gagaacgaga acgacgacga ggcccggctc
     7381 atggcaccct cacgctcacg ccttgccttc gaggcggcgg cggaccaggt cgagggcggt
     7441 ggtggtggcc caggcgcgca cctgggcgcg gtcgccgggg gcgcgggtgc tgcgggtcac
     7501 ggtgccgtcc gggtcggcga cgccgaggca gaagctgccg accggcttgt tgtcctgctc
     7561 ggtggggccg gcgacgccgg tggtggcgat gccgacgcct gcgccgaaca ggcgccgcac
     7621 gccctcggcc atggcacccg cgaccggctc gctgaccggc ccatgctcgg cgagcagggc
     7681 ggcgtcgacg cccagcagct cggctttggc gtcggtggcg tagacgacga ccccgccgag
     7741 gtagtagtcg ctggcgccgg cggcggcggt gatgcggccg ccggccaggc cgccggtgag
     7801 tgactcggcg ctggcgaggg tctgcccggc ggcgcgcaac agccgcccga cggccgcctc
     7861 cagggtctca tcgtcgagac cgtagacgag gtcgccgagc tccttgcgga ccgcctcctc
     7921 gaccggcgcg atggtggcga gagcctgctc gcgggtggcc ccgacggtgg tgagccgcac
     7981 ccggacctcg ccggggctgg ccaggtaggc gagcgtcacc ccggcctcct cgcccatcga
     8041 ctcccacagg ggggtgagcc gctcggccag ggccgactcc cccaacccgg cggtgcgcag
     8101 ggtgcgcgag acgatcgcgc ggccctcccc cgcccgggcg gcgagatccg gcagcaccac
     8161 ccgttcgagc atgccctcca tttcgcgggg gacgccgggg accgcgtaga gcggcttgcc
     8221 gccgacctcg atgatcaggc cgggcgcggt gccgcgcggg ttgtcgatgg cgcgtgcgcc
     8281 ctctggcacg tcggcctggc gcaggttcat ctcgggcatg gcccggccga gccgctggaa
     8341 cttctgccgg aggaagtctt cgatctcggg ccgcctgatc aatggcacgc cgagggccgc
     8401 ggccaggccc tccctcgtga tgtcgtcctg ggtcggaccg agacctccgg tggcgatcac
     8461 cacgtcggcg cgggagaggg ccagggagaa ggcctcgcgg atgcgatcct cgttgtcccc
     8521 caccgcctgg tggtacacca cgtccacgcc gatctcgccc agccgctcgg agatccactg
     8581 cgcgttgctg ttcgagatct ggccgaggag gatctccgtt ccgaccccga cgatctcggc
     8641 cctcatcgcg gtgcgggggc ggccggcgcg cggagccacc gcgttccccg aaccaggtac
     8701 tcgaggccgg tggccgccgt caaagcgatg gccacgacga gcaccgccag ccgggcaccg
     8761 tgcgccgacg gccccaacgg cagcacgtag agcgtgatag ccgtgagctg caacgtggtc
     8821 ttgagcttcg ccgaccgaga ggccgggagg gcgatccccc gcagcccgag taccacgcga
     8881 agcaccgaga ttgccgcctc gcgaacgagg aagatcgcca gcgcccacag ggggaactca
     8941 cccatcgagg ccagcgtgat gatcggggcc gccacgagaa ccttgtccgc cagggggtcc
     9001 agccactgtc ctgtgcgcga caccgacccg taccggcggg cgatgtatcc gtcgacgccg
     9061 tcggtcgcac cacctagcac gaacagcacg gcggcggcgt ccctgtcccc gtccccggcc
     9121 aggatcagcc acacgatggc cgggacgagc aatatgcgga gcgcggagat cacgttcggc
     9181 cagccgaacc cgaacgggct cagcgagcca gctgcgccgg ccgcctcccg ctcgctcacc
     9241 ggctcacggg gtc
//
LOCUS       DASWDC010000202         3753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1468090, whole genome shotgun sequence.
ACCESSION   DASWDC010000202 DASWDC010000000
VERSION     DASWDC010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468090"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VF995_06550"
     CDS             complement(<1..839)
                     /locus_tag="VF995_06550"
                     /EC_number="2.6.1.1"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate transaminase/aspartate
                     4-decarboxylase"
                     /protein_id="HEX9343259.1"
                     /translation="MTTTRRDKDPQHLSRQQERELQQLSPFELKSKLIELAEEHQRAS
                     AATMLNAGRGNPNWTATTPRAAFFLLGRFGLEEARRDWDEWPGLGGMPGRDGIAARLA
                     AFLDANDRDPGADLLRRAVAYATTKLGLDADTFVHELTDGIIGDNYPVPDRMLRCAER
                     VVHTYLVKEVCAGKPPRVPFDLFATEGGTAAMCYIFDSLATNQLLHTGDKIAIMAPIF
                     TPYLEIPRLERYRFDVLELRATRTSQDGFHTWQYPDSELDKLADPSVKALFLVNPSNP
                     PSV"
     gene            complement(1016..1972)
                     /locus_tag="VF995_06555"
     CDS             complement(1016..1972)
                     /locus_tag="VF995_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343260.1"
                     /translation="MSVFEPDTTDRSRDATELPNFPVVLRGYDRQQVTAYVEELAANL
                     EGERQRADQAERHMAQMQLEISPARNQPPPTFEHLGAQAARVLEQAGMSAERLVEEAQ
                     VRADGIVADAQAQSSDLVDAAEQQAEQLDREAAQRLAEAGRQRDRMLAAAEHDAEQVR
                     AGADADARRMLAEARTLAERIRREAEEERAATEAEADQLQDIHDSLLRHLSQVHGELG
                     DVLAARGDGGHEEPAADPARAPQEQAPAGKGQGGGPSASGGTATAAETAPSARHDQRD
                     QASATHRDQPTQALQGAHSASRPSHAIERAPVTPTRRASGRG"
     gene            2151..2723
                     /locus_tag="VF995_06560"
                     /pseudo
     CDS             2151..2723
                     /locus_tag="VF995_06560"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
     gene            2985..3626
                     /locus_tag="VF995_06565"
     CDS             2985..3626
                     /locus_tag="VF995_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9343261.1"
                     /translation="MDARISLRVDGLVRDVIVDTRTSLLDLLREHLGVTGPKKGCDQG
                     QCGACTVLLDGRRVNSCLALAVAHDGGEVATVSGLAALAQAQAGAHDGDGDGDGDGDG
                     DRDGEAAAALHPLQRAFIRHDAFQCGYCTPGQLCSAVGMLAEVAAGWPSVVSADLCAP
                     GPAALDEAEIRERMSGNLCRCGAYVSIVQAVARAAQADSGGTAETTGTKEAAA"
     gene            3623..>3753
                     /locus_tag="VF995_06570"
     CDS             3623..>3753
                     /locus_tag="VF995_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011027599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX9343262.1"
                     /translation="MREFGYQRVFDVPGAVAVLGAAPDARFLGGGTNLIDLMKAGVER
                     "
BASE COUNT          463 a         1283 c         1424 g          583 t
ORIGIN      
        1 attaccgacg gcgggttgct cgggttgacc aggaacagcg ccttgaccga cgggtcggcg
       61 agcttgtcca gctcgctgtc gggatactgc caggtgtgga agccgtcctg gctggtgcgg
      121 gtggcccgca gctccagcac gtcgaagcgg tagcgctcca ggcgcgggat ctcgaggtag
      181 ggggtgaaga tcggcgccat gatggcgatc ttgtcgccgg tgtgcagcag ctggttggtc
      241 gccagcgagt cgaagatgta gcacatcgcg gcggtgccgc cctcggtggc gaacaggtcg
      301 aaggggaccc gcggcggctt gccggcgcag acctccttga ccaggtaggt gtggaccacc
      361 cgctcggcgc agcggagcat gcggtcgggc accgggtagt tgtcgccgat gatcccgtcg
      421 gtcagctcgt ggacgaaggt gtcggcgtcc aggccgagct tggtcgtggc gtaggcgacc
      481 gcgcggcgca gcaggtcggc gccggggtcg cggtcgttgg cgtcgaggaa ggcagcgagc
      541 cgggcggcga tgccgtcgcg ccccggcatg ccgcccaggc ccggccactc gtcccagtcc
      601 cgccgcgcct cctccagccc gaagcggccc agcaggaaga aggcagcgcg cggggtggtg
      661 gcggtccagt tcgggttgcc gcggccggcg ttcagcatgg tggccgcgct ggcccgctgg
      721 tgctcctcgg ccagctcgat cagcttggac ttcagctcga aggggctgag ctgctgcagc
      781 tcacgctcct gctggcggga gaggtgctgc gggtccttgt ccctgcgggt ggtggtcatg
      841 cgcgagcccc cggtcgagtg gcgccccagc cgctgctgga tgtctggcaa ccaaatcgac
      901 gcgggaaggc aagcgcttga tctcctcccg tcgggaccga ggaggctcgg cgggatatct
      961 ggctatttgt cgccagcctg cgctggcgac ctcggcacta gccccagcct cagcgtcagc
     1021 ctctgccgct ggcgcggcgg gtcggggtga ccggcgcccg ctcgatggcg tggctgggcc
     1081 gggaggcgga atgcgcgccc tgcagcgcct gggtgggctg gtcccggtgg gtggccgacg
     1141 cctggtcccg ctggtcgtgc cgtgccgagg gcgccgtctc ggcggccgtg gccgtgcccc
     1201 cgctggcgct gggaccgccg ccctggccct tgccggccgg cgcctgctcc tgcggtgcgc
     1261 gggcggggtc cgccgccggc tcctcgtggc cgccgtcgcc cctggccgcg agcacgtcgc
     1321 ccagctcgcc gtggacttgg ctgaggtggc gcagcaggct gtcgtggatg tcctggagct
     1381 ggtcggcctc ggcctcggtc gcggcgcgct cctcctcggc ctcgcggcgg atgcgctcgg
     1441 ccagggtgcg ggcctcggcc agcatccggc gggcgtcggc gtcggctccc gcgcggacct
     1501 gctcggcgtc gtgctcggcg gcggcgagca tccggtcccg ctgccggcca gcctcagcga
     1561 ggcgctgggc ggcctcccgg tcgagctgct cggcctgctg ctcggcggcg tcgaccaggt
     1621 cggaggactg cgcctgggcg tcggcgacga tcccgtccgc gcggacctgc gcctcctcga
     1681 ccagccgctc ggcggacatg ccggcctgct cgaggacgcg ggcggcctgc gcgccgaggt
     1741 gctcgaaggt gggcggcggc tggttgcggg cggggctgat ctccaactgc atctgcgcca
     1801 tgtgccgctc ggcctggtcg gctcgctggc gctcgccctc caggtttgcg gccagctcct
     1861 cgacgtaggc ggtgacctgc tggcgatcgt agccgcgcag caccaccggg aagttcggca
     1921 gctcggtcgc gtcccgcgac cggtcggtgg tgtccggctc gaacacgctc atgtgggctc
     1981 tccctgaaga taagacagaa acacggcata catgcaaaag cgcgtcgtat cgagcgtgcc
     2041 tcccgactcg accatatagc ccggatcggg agagagcggt cgcctggctc agaacttcgc
     2101 tgcgggggaa ttgtcccgtc caagcctggc ccgtggcaaa tccgttgagc atgacattcc
     2161 cgctaagttc agctcctgac caatatggcc gaaccaatca gaaacgagca ggaggctgtg
     2221 tgtccggcac gttcagtgtc cccctgccag tggacctcgc cgccgttggg atcggcgcgg
     2281 tgcagggggc ggtgtttggc aaccgcatgg cgcgcgagcg ccacatcgac ctcgtcggca
     2341 tcgccagcat cggcatcgcc accggcgtcg gcggcggcat gcttcgcgac ctgctgctgg
     2401 agacgcaggt cgcagcgctc ggctccaacc gctacctggc gaccgcggtg ctcgccgccc
     2461 tggtcggcat gctcttcgcc gacctggtca accgcatgga gctggcggtg gtcgcgatcg
     2521 atgcggcctc gcttgggatc tgggccgcgg tcggtaccta caaggcgaac ggcttcggcc
     2581 tcggcgtggt cccaagcgtg ttcgtcggcg tgatcgcctg caccggcgga accatcatcc
     2641 gcgacctgct gctgcgcacc gaggtctcgg tggtgcgggt cgggtcgttc tattcgatcg
     2701 ccgccctcgg cggcagcacc accttcctgg cggtggagcg cctctccgac agcgccaccg
     2761 ccgcggtcgc ggcgatcctg gtcaccttcg ggcttcggat gctcgcctac ttcttcggct
     2821 ggcggacgcc ccgctcccga gcccttccca tcccccacgg cggcgccaag gacaagcacg
     2881 aggacaaggg caaggatcac cgccagcagc cgccggtgac cggaacggcg ctagcctgtc
     2941 cgtcatcgcg gggatacgcg ggtgcgagga aggaggcgtg ctggatggac gcgaggatca
     3001 gcctccgggt ggacgggctc gtgcgggacg tgatcgtgga cacccggacc agcctgctgg
     3061 acctgctgcg cgagcacctc ggggtgaccg gcccgaagaa gggctgcgac cagggccagt
     3121 gcggcgcctg cacggtcctg ctcgacgggc ggcgggtgaa cagctgcctg gccctggcgg
     3181 tggcgcacga cggcggcgag gtcgccaccg tctcggggct ggccgccctg gcccaggccc
     3241 aggccggcgc gcacgacggc gatggcgacg gcgacggcga tggcgacggc gaccgtgacg
     3301 gcgaggcggc ggcggcgctg catccgctgc agcgggcgtt catccgccac gacgcgttcc
     3361 agtgcggcta ctgcactccc ggccagctct gctcggcggt ggggatgctg gccgaggtcg
     3421 ccgcgggctg gccgagcgtg gtgagcgcgg acctgtgcgc gccgggcccg gcggcgctgg
     3481 atgaggccga gatccgtgag cggatgagcg gcaacctgtg ccgctgcggc gcatacgtgt
     3541 ccatcgtcca ggcggtcgcg cgggctgcgc aggccgactc cggcggtacc gctgagacca
     3601 ccgggaccaa ggaggccgcg gcatgaggga gttcggctat cagcgggtct tcgacgtgcc
     3661 cggagcggtc gccgtgctcg gcgccgcccc ggacgcacgc ttcctgggcg gcggcaccaa
     3721 tctcatcgac ctgatgaagg ccggggtgga gcg
//
LOCUS       DASWDC010000203         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1052778, whole genome shotgun sequence.
ACCESSION   DASWDC010000203 DASWDC010000000
VERSION     DASWDC010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052778"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VF995_06575"
     CDS             <1..787
                     /locus_tag="VF995_06575"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX9343263.1"
                     /translation="GLTTAPAAEAPKGAKLVILAVLDDELPSLITALAAEGAFEPGQA
                     VIHTAGVDGPELLTPASAIGALIAACHPVQTFTDDLDATLGRIPGTVWGVTGDPVGRE
                     MVVMLGGRPMDVPASGRVRYHAALVLAANGAAALAASAADILRAGGLIDPAAVVGPLI
                     HASVDSALATGQAGMSGPWVRGEGRTVGMHTEALARKQRVIKVYAALARLVADRAAAA
                     GRLDPEARRRVEAALQSSDGEDSRERLELLRRVARRARGREQR"
     gene            762..1631
                     /gene="panC"
                     /locus_tag="VF995_06580"
     CDS             762..1631
                     /gene="panC"
                     /locus_tag="VF995_06580"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX9343264.1"
                     /translation="MRAAESSAELAAATAEIREGGGRVGLVPTMGALHAGHRALLAQA
                     RRERDFVVASIFVNPLQFGPGEDLASYPRDRERDLAVLEEEGADLAFVPPEREVWPDG
                     EPAVRLAVGGTLGTTLEGAHRPGHLDGVATVVAKLLHLVGPSAAYFGQKDAQQLAMIR
                     RMVADLAFPHEVVAVPTVREHDGLALSSRNAYLSPLERVTAGALYRALQAGRAAWHAG
                     KTQAAEVEGAARTLLAATAGVDAQYVALVEPDDFRPAEAARAGQILATAARVGPARLI
                     DNVILSNEQEGWP"
     gene            1631..2455
                     /locus_tag="VF995_06585"
     CDS             1631..2455
                     /locus_tag="VF995_06585"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX9343265.1"
                     /translation="MLLAIDVGNTEITIGVFDGDTLVQHWRAATVAERTADETALLLS
                     GFLGQEGLDLSTKISGVAVSSVVPAVTAALREMVRRYCAGFEALVIEPGVRTGLSILV
                     ENPREVGADRVVNTAAAFAAYGGPAVVVDFGTATTFDAVSERGEYLGGAIAPGIQIST
                     NALATHTAQLHRVELVAPRSVVGKSTTECLQSGIVYGFAGQVDGIVRRMVAELGGQVT
                     VIATGGLASAVLAACETIQCHDPWLTLQGLRIVYGRNQSPPQAPAAGQGRDREPTA"
     gene            2439..3092
                     /locus_tag="VF995_06590"
     CDS             2439..3092
                     /locus_tag="VF995_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343266.1"
                     /translation="MSQRPDRRPDRVAGRPLSPRAFRTFGIEAVVLGSPTRQEGLLRR
                     DGVALWRRLDAHAPLFDRVADETPEGVAGAVSAAAGLLPIRVVTAATPGRDRGMRVPA
                     LGERATRALQLASGPPPRAGWLLLVDEGPGETWLYLADEQDPADALREFLGRRGRTDF
                     GRVRRRSRAGGLGSAGLLLGLVLIVWSGALGLGLAGQLLGVAALFGSITALVRARPR"
     gene            3165..>3565
                     /locus_tag="VF995_06595"
     CDS             3165..>3565
                     /locus_tag="VF995_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX9343267.1"
                     /translation="MADPEYPSEAEHRSAVEQARRDKLARLRKAGVDAYPVGFERSHT
                     LAQVAEGWKDLAAGEESDDVVSVAGRIVLKRSMGKLGFWTLRYGDDELQVMLNQAVLG
                     PERFGFAEELDAGDWAGVEGPVIRTRRGELSV"
BASE COUNT          439 a         1349 c         1316 g          461 t
ORIGIN      
        1 cggcctcacc accgcgccag ccgcggaggc gcccaagggt gccaagctgg tcattctcgc
       61 cgtgctggac gacgagctgc cgtcgctgat caccgccctg gcggccgagg gcgccttcga
      121 gcccggccag gcggtgatcc acaccgccgg cgtcgacggc ccggagctgc tcaccccggc
      181 cagcgccatc ggcgcgctga tcgccgcctg ccacccggtg cagacgttca ccgatgacct
      241 cgacgccacc ctcggacgca tccccgggac cgtctggggc gtgaccggcg atccagtcgg
      301 ccgggagatg gtggtcatgc tcggcggccg gcccatggac gtgccggcct cgggacgggt
      361 ccgctaccac gccgccttgg tgctcgccgc caacggcgcc gccgcgctgg ccgccagtgc
      421 cgccgacatc ctcagggccg ggggtctgat cgacccggcg gcggtggtcg gcccgctgat
      481 ccatgccagc gtcgactccg ccctcgccac cgggcaggct ggcatgtcgg ggccgtgggt
      541 gcggggggag ggccgcaccg tcggcatgca caccgaggcg ctcgcccgca agcagcgggt
      601 gatcaaggtc tacgcggcgc tcgcccgcct ggtcgccgac cgcgccgccg ccgccggccg
      661 cctggacccg gaggcccgcc gccgggtcga ggccgccctg cagagcagcg acggcgagga
      721 ctcgcgcgag cggctggagc tgctgcgaag ggtggcgcgg cgtgcgcgcg gccgagagca
      781 gcgctgagct ggccgccgcc accgccgaaa tccgcgaggg cggcggccgg gtcggcctcg
      841 ttcccaccat gggtgcgctg cacgcgggcc accgcgccct gctcgcccag gcgcggcggg
      901 aacgcgactt cgtggtcgcc tcgatcttcg tcaacccgtt gcagttcgga ccgggcgagg
      961 acctcgccag ctacccgcgc gaccgggaac gcgacctcgc cgtcctcgag gaggagggcg
     1021 ccgacctcgc cttcgtcccg ccggagcgcg aggtctggcc agatggcgag cccgcggtgc
     1081 gtctcgccgt gggcggcacg ctcggcacca ccctggaggg cgcccaccgc cccggccatc
     1141 tcgacggggt cgccacggtg gtggccaagc tgctgcacct ggtcggcccg agcgcggcgt
     1201 atttcgggca gaaggacgcc cagcagctcg ccatgatccg ccgcatggtc gccgacctcg
     1261 ccttcccgca cgaggtcgtg gccgtcccca ccgtccgcga gcacgacggg ctggctctct
     1321 cctcccgcaa cgcctacctg tcgccgctgg agcgggttac cgccggcgcc ctctaccggg
     1381 cgctgcaggc cggcagggcc gcctggcacg ccggcaagac ccaggcggcc gaggtagagg
     1441 gcgccgcccg gaccctgctg gcggcgaccg ccggcgtcga cgcgcagtac gtcgccctgg
     1501 tcgagccgga cgacttccgc cccgccgaag cggcccgcgc cggccagatc ctcgccaccg
     1561 ccgcccgggt cggccccgcg cgcctgatcg acaatgtcat cctgagcaac gaacaagagg
     1621 ggtggccctg atgctgctcg cgatcgacgt cggcaacacc gagatcacca tcggcgtgtt
     1681 cgacggcgac accctggtcc agcactggcg cgccgccacg gtggctgagc gcaccgccga
     1741 cgagaccgca ctgctgctgt ccgggttcct cggccaggag ggcctggacc tcagcaccaa
     1801 gatcagcggg gtggccgtct cctcagtggt gccggcggtc accgcggcgc tgcgcgagat
     1861 ggtgcgccgc tactgcgccg gcttcgaggc gctggtgatc gagccgggcg tgcgcaccgg
     1921 cctgtcgatc ctggtggaga acccgcgcga ggtcggcgcc gaccgggtgg tcaacaccgc
     1981 cgccgcgttc gccgcctacg gcggcccggc ggtggtggtc gacttcggca ccgccaccac
     2041 cttcgacgcc gtctccgagc gcggcgagta cctcggcggg gcgatcgcgc cgggcatcca
     2101 gatctccacc aacgccctgg ccacccacac cgcccagctc caccgggtcg agctggtcgc
     2161 gccccgctcg gtggtcggca agagcaccac cgagtgcctc cagtccggca tcgtctacgg
     2221 cttcgccggc caggtggacg gcatcgtccg ccggatggtc gccgagctgg gcggccaggt
     2281 caccgtgatc gccaccggcg gcctcgccag cgccgtactg gccgcctgcg agaccatcca
     2341 gtgccacgac ccgtggctga ccctgcaggg cctgcgcatc gtctacggcc gcaaccagag
     2401 ccctccgcag gcgccggccg ccggtcaggg tcgcgaccgt gagccaacgg cctgaccggc
     2461 ggcctgatcg ggttgccggc cggccgctgt cgccgcgggc gttccggacc ttcggcatcg
     2521 aggcggtggt gctcggctcg ccgacccggc aggagggctt gctccgccgc gacggcgtgg
     2581 cgctgtggcg gcgcctggac gcccacgcac ccctcttcga ccgggtcgct gacgagacgc
     2641 cggagggcgt ggcgggcgcg gtgagcgccg ctgcggggct gctcccgatc cgggtggtga
     2701 ccgccgccac gccagggcgt gaccgcggca tgcgcgtccc ggcgctcggc gagcgcgcca
     2761 cccgcgccct ccagctggcc agcgggccgc caccgcgggc cggctggctg ttgctggtcg
     2821 acgaggggcc gggggagacc tggctctacc tcgccgacga gcaggatccc gccgacgccc
     2881 tgcgggagtt cctcggccgc cgtggccgca ccgacttcgg acgggtccgg cgccgctccc
     2941 gcgccggcgg gctcggcagc gccggcctgc tgctcggcct ggtcctgatc gtctggtcgg
     3001 gcgccctcgg cctcggcctg gccgggcaac tgctgggcgt ggccgcgctg ttcgggtcga
     3061 tcacggcgct ggtccgcgcc cgcccccggt gaggtgcctg cccaagctcc aaagatgggt
     3121 aggattcgag gcgaacgtag cgaccatccg gaggaaaaga accagtggcc gaccctgagt
     3181 acccgtccga ggccgagcac cgttccgccg tggagcaggc gcggcgcgac aagctcgcga
     3241 ggctgcgcaa ggccggggtt gacgcctacc cggttggctt cgagcgcagc cacaccctgg
     3301 cgcaggtggc cgagggctgg aaggacctgg ccgctggcga ggagtccgac gacgtcgttt
     3361 cggtggcagg gcggatcgtg ctgaagcgtt ccatgggcaa gctcggtttc tggacgctgc
     3421 ggtacggcga cgacgagctg caggtgatgc tgaaccaggc ggtcctcggc cccgagcggt
     3481 tcggcttcgc cgaggagctc gacgcgggtg actgggcggg tgtcgaaggg ccggtgatcc
     3541 gcacccggcg gggcgagctg tcggt
//
LOCUS       DASWDC010000204         6979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1384214, whole genome shotgun sequence.
ACCESSION   DASWDC010000204 DASWDC010000000
VERSION     DASWDC010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384214"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VF995_06600"
     CDS             <1..969
                     /locus_tag="VF995_06600"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX9343268.1"
                     /translation="TGVCATVDEARKDLEGTLAELRTFVEPLGMNLMCSGTHPFSDWA
                     AQQISPNPRYAQLVEDMQWLARRLQIFGIHVHVGVRSPEKAVAIVNALTGYIPHFLAL
                     SASSPYWKGRDTGLASCRSKVFEGLPTAGLPYHLTGWAQFEQFMDTLISAKTIHTIRE
                     VWWDIRPHPNFGTVELRICDGLPTMSEIATVAAMSQCLVDRMNTLIDRGYTLPVPRGW
                     IVRENKWRAARYGLDAHIILDDEGRLQPVREAIEELVEELAPTARRLGCLDELHDALQ
                     LMERGPSYIRQREVVAAGGDLTDVVDTLVTELRTDQPTPIPEQPEP"
     gene            966..2144
                     /locus_tag="VF995_06605"
     CDS             966..2144
                     /locus_tag="VF995_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017584071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9343269.1"
                     /translation="MILDLSSFIASHEAELIHLRRHLHAHPELGRAERETTALVARRL
                     EAAGLRPRLLPKGTGLTCDIGDGVGPVVGLRADIDALPLQDEKDVAYRSTVPGVCHAC
                     GHDVHTTMLLGAGLALAEHAADLRGKVRLVFQPAEEKNPGGALDVIEAGAVDGVAAIF
                     ALHCDPKVDVGRIGTRVGPITAASDLFEVRLTGPGGHTARPHLTADLTYIVAKLITEL
                     PAALSRLVDPRATLTVSFGSVQSGTVANVIPTSALARGTVRVLDRNTWGDAPKLLERV
                     LEAVVAPYSVDYQLGYHRGSPPVVNDEAATVVLSNAARIAIGPEAVLSAPQSMGGEDF
                     SWYLETIPGSMGRLGVRIPGTDLDIHAAHFDVDERSIAVGVRILAQAAVDALEHYAKA
                     "
     gene            2249..3097
                     /locus_tag="VF995_06610"
     CDS             2249..3097
                     /locus_tag="VF995_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9343270.1"
                     /translation="MPLAVLFGLGSSLCWGVSDFFGGVLSRRLSALAVALWSQAGGGV
                     VLAVGVLALGGPPTLRGFLLGAAGGIGGGAGLLLFYRALAEGAMSIVAPLSACGAAVP
                     VAVAVAGGEVPSPLALAGMAVAFAGVVLVSRSTDAEQHPSGRPALVVALSFGAALGFG
                     WYFVMLHAGSVAGGSPLWTIMGGRASSLSVLAGITLLGRKPAPWPGRGIAPLTLVGSV
                     DAFANLLFAVASTKGNLGVVGVLGSLYPVATVLLARFVLAERLSRVQGAGVALALAGV
                     ALLATG"
     gene            3234..4031
                     /gene="hisN"
                     /locus_tag="VF995_06615"
     CDS             3234..4031
                     /gene="hisN"
                     /locus_tag="VF995_06615"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008741497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX9343271.1"
                     /translation="MNPNLNFALELADLADKLTMERFRADDLVVETKPDMTPVTEADR
                     AVEQALRHRIAEEYPTHAVVGEEFGAEPGNEAGNGAGNGAGDGAPRWILDPIDGTKNY
                     VRGIPVFATLIALECDGVGQLGVVSAPALGRRWWASRGQGAYADGRRMRVSRVAELGD
                     AQLCHSTFDEWEAVGLGERFLDLLRACWRERGFGDFWIHMLVAEGAAEIAAEPTVAHW
                     DVAPIQVIVEEAGGRFTDLGGTPRADTGSAVSTNGLLHDQVLEALRP"
     gene            complement(4051..5633)
                     /locus_tag="VF995_06620"
                     /pseudo
     CDS             complement(4051..5633)
                     /locus_tag="VF995_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
     gene            complement(5596..>6979)
                     /locus_tag="VF995_06625"
     CDS             complement(5596..>6979)
                     /locus_tag="VF995_06625"
                     /inference="COORDINATES: protein motif:HMM:NF042897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6777 domain-containing protein"
                     /protein_id="HEX9343272.1"
                     /translation="GGSIWGAGLFGANQAQAREIILQASGQVGTNPFTHSVTPSKPKR
                     FGLGAIGTATQARQGVLRLHGDQVGLYGGTQNQSTCNRAQMIAFLQQNPTKAKAWVAA
                     LNRDPTLHWSGGNKLTVAQIGAYINELTPVLLRLDTRVTNNGYRDGHATPFQAVLQAG
                     TAVLIDKLGVPRSRCACGNPLLEPTDLTKTSQYSGAAWPGFKAATIVTVAPAPRQIAN
                     VMLVDVDKGTSAVFDRTTGSAGTKDVSVKVTAKLKNEINLTLILDGAQLVAPPPSTEP
                     PASDTTAAGPTDTSGSGGSPTETTVSQPGGSETTTTIGPGGGATSSSGTETSSSSSTS
                     SSSETTTQTTTGATDTSTTNDTSTTIGSTSSSNVTTTSLETTTTTPPPSTSTSEATTS
                     SSSATSTSANATAGAVIPAVYLKGVNAQLPSQPGYRVAFSGTWGGWRQGWTRCESHHE
                     RPDRSSARTI"
BASE COUNT          878 a         2477 c         2574 g         1050 t
ORIGIN      
        1 accggcgtct gcgccaccgt ggacgaggcg cgcaaggacc tggaggggac cctcgccgaa
       61 ctgcgcacct ttgtcgagcc gctcgggatg aacctgatgt gctccggaac ccatccattc
      121 tcggactggg cggcacaaca gatcagtccc aatccgcgct acgcgcagct cgtcgaggac
      181 atgcaatggc tggcgcggcg gctgcagatc ttcggcatcc acgtgcacgt cggcgtgcgc
      241 tcgccggaaa aggccgtcgc gatcgtcaac gcgctgaccg gatatatccc gcatttcctc
      301 gcgttgtcgg cgtccagtcc ctactggaag ggccgcgaca ccggcctcgc ctcctgccgc
      361 agcaaggtct tcgagggcct gcccaccgcc ggcctgccct accacctgac cggctgggcg
      421 cagttcgagc agttcatgga cacgctcatc tcggcgaaga ccatccacac catccgggaa
      481 gtctggtggg atatccggcc ccaccccaac ttcggcacgg tcgagctgcg catctgcgac
      541 ggcctgccga ccatgagcga gatcgccacg gtggcggcca tgagccagtg cctggtggat
      601 cggatgaaca ccctgatcga tcgcggctat accctaccgg tccctcgggg ttggatcgtg
      661 cgcgagaaca agtggcgcgc ggcccgctac gggctcgacg cgcacatcat tctcgacgac
      721 gaggggcgcc tgcagccggt gcgcgaagcc atcgaggagc tcgtcgaaga gctcgccccg
      781 acggcgcgcc ggctcggctg cctcgacgag ctgcacgacg cgctccagct catggagcgc
      841 ggcccgagct atatccgcca acgtgaggtg gtagctgcgg ggggcgacct gaccgacgtg
      901 gtcgacacgc tggtcaccga gctccgcacc gatcaaccca cgccgatccc cgagcagccc
      961 gaaccgtgat cctagacctc agcagtttca tcgcgtccca cgaagcagag ctgatccacc
     1021 tccggcgcca cctgcacgcc caccccgagc tcggccgggc ggagcgggag acgaccgccc
     1081 tggtcgccag gcggctggag gccgccggcc tgcgtccccg cctgctgccc aagggcaccg
     1141 gcctgacctg tgacatcggc gacggcgtcg gcccggtggt gggcctgcgc gccgacatcg
     1201 acgccctgcc gctgcaggac gagaaggacg tcgcctaccg gtccaccgtc ccgggcgtct
     1261 gccatgcctg cggccacgac gtccacacca ccatgctgct cggcgccggc ctcgccctcg
     1321 ccgagcacgc cgccgacctg cgcggcaagg tccggctggt gttccagccg gccgaggaga
     1381 agaacccggg cggcgcgctg gacgtgatcg aggccggggc ggtcgacggc gtcgccgcga
     1441 tcttcgcgct gcactgcgac cccaaggtcg acgtcggccg gatcggcacc cgggtcggcc
     1501 cgatcaccgc cgcctccgac ctgttcgagg tgcgcctgac aggacccggc ggccacaccg
     1561 cccggccgca cctgaccgcc gacctcacct acatcgtcgc caagctgatc accgagctgc
     1621 ccgcggccct gtcgcggctg gtcgacccgc gcgccaccct gaccgtcagc ttcggctccg
     1681 tgcagtccgg cacggtcgcc aacgtcatcc cgaccagcgc gctggcgcgc ggcaccgtgc
     1741 gggtgctgga ccgcaacacc tggggcgacg cccccaagct gctggagcgg gtgctcgagg
     1801 cggtggtcgc gccctacagc gtcgactacc agctcggcta ccaccgcggc tcgccgccgg
     1861 tggtcaatga cgaggcggcc acggtggtgc tgtccaacgc ggcccgcatc gccatcggcc
     1921 cggaggcggt cctcagcgcg ccgcagagca tgggcggcga ggacttctcc tggtacctgg
     1981 agaccatccc aggttccatg ggccgcctcg gtgtccgcat cccaggcacc gacctggaca
     2041 tccacgcggc gcatttcgac gtggacgagc gttccatcgc ggtcggggtc cgcatcctcg
     2101 cccaggccgc cgtggacgcg ctggagcact acgccaaggc gtagacgccg ttcccgccct
     2161 ccctggccgc atcgctggcc gccgcgccgc gcacgtacag tgtctggcgg tcgaccgatc
     2221 gaccaccgat cgaccaggga gggcgggttt gccgctggcg gtgctgttcg ggctggggtc
     2281 cagcctgtgc tggggggttt cggacttctt cggcggggtg ctgtcgcggc ggctgtcggc
     2341 gctggcggtg gcgctgtgga gccaggccgg tggcggcgtg gtgctggcgg tcggcgtgct
     2401 cgccctgggc gggccgccga cgctgcgcgg gttcctgctg ggcgccgccg gcgggatcgg
     2461 cggcggcgcc gggctgctgc tgttctaccg ggcgctggcc gagggcgcga tgtcgatcgt
     2521 ggcgccgctc tcggcctgcg gcgccgcggt gccggtggcg gtcgcggtgg ccggcgggga
     2581 ggtgccgagc ccgctggcgc tggcggggat ggcggtcgcc ttcgccgggg tggtgctggt
     2641 gtcgcgttcc accgacgccg agcagcaccc gagcggccgg cccgcgctgg tggtggcgct
     2701 gtcgttcggc gccgcgctgg gcttcggctg gtatttcgtc atgctgcacg ccggcagcgt
     2761 cgcgggcggc tccccgctgt ggacgatcat gggcggccgg gcctcctcgc tgtcggtgct
     2821 cgccggcatc accctgctcg gccgcaagcc ggcgccctgg ccgggtcgcg ggatcgcccc
     2881 gctcaccctg gtgggcagcg tcgacgcctt cgccaacctg ctgttcgcgg tcgcctccac
     2941 caagggcaac ctcggcgtgg tcggggtgct cggctcgctg tacccggtgg cgacggtgct
     3001 gctggcccgc ttcgtgctgg ccgagcgcct ctcccgggtc cagggcgcgg gggtggcgct
     3061 cgcgctggcg ggcgtggcgc tgctcgccac cgggtagccc cgggcttgga cacggacacg
     3121 gacacggaca cgggcgtggg acacggacac gttgtggcaa agccacacga gcgttaccag
     3181 aacgcagcgc aaggtttgcg gaacccgcag gtcggcgcgg tacgctcccg gctatgaacc
     3241 cgaatctcaa tttcgccctc gagttggccg acctggccga caagctcacc atggagcgct
     3301 tccgcgccga cgacctggtc gtcgagacca agccggacat gacccccgtg accgaagcgg
     3361 accgggccgt cgagcaggca ttgcgccacc ggatcgccga ggagtacccc acccacgccg
     3421 tcgtcggcga ggagttcggc gccgaacccg gcaacgaagc cggcaatgga gccggcaatg
     3481 gagccggcga cggagccccg cgctggatcc tcgacccgat cgacgggacc aagaactacg
     3541 tgcgcggaat ccccgtgttc gccacgctga tcgcgctgga gtgcgacggt gtggggcagc
     3601 tcggggtggt ctcggcaccc gccctcgggc ggcgctggtg ggcttcgcgc ggccagggcg
     3661 cctacgccga cgggcgccgc atgcgggtct cgcgggtggc cgagctgggt gacgcgcagc
     3721 tgtgccactc gacctttgac gagtgggagg ccgtcggcct gggcgagcgc ttcctcgacc
     3781 tgctccgcgc ctgctggcgc gagcgcggct tcggcgactt ctggatccac atgctggtgg
     3841 cggaaggcgc cgccgagatc gccgccgaac cgaccgtggc ccactgggac gtcgccccga
     3901 tccaggtgat cgtcgaggag gccggcggtc gcttcaccga cctcggcggc actccccgcg
     3961 ccgacaccgg cagcgccgtc tccaccaacg gcctcctcca cgaccaggtc ctggaggccc
     4021 tccgcccgta acccgtcggg cttcgcggcc tcagcggcgg cggcgcacga aggcagcgcc
     4081 cgccagcatg aggccgccga ccgaccaggg ccagagcata cccgcgccag cggctgccac
     4141 gaccgtcggg gtggtgaggg tgaccgtcgg ggtggtgacc gtcagcgtag tgctgatcag
     4201 cgtggtggtg gtggcctgag tggtcgaggg cggcgaggtg gtgcgcgtcg gcttggtcgt
     4261 gggcgctggc ccgctcgtgg tggccggtgg cgcggtcgtc ggcgtgccgg tggtcgagcc
     4321 cggggcgcgg gtgtgcgagg tgctgaccgc cgcgccggga gcggccggcc ggcccgacga
     4381 ctgggtggcg gcgttcgccc cgcgctggcc cggcggcccg gagccgacca ccaccgaagg
     4441 gccgctgccg ccgccgctgc cggcactgac cccgggcgcc ctcccggcct gccgggagaa
     4501 cccccacacc gccaccgcgg ccagcaggac cccggccgtg gccagcgcga gccgcccccg
     4561 ccagcgcggc cgcgcggccc cagccgaacg gtccgggctc cccgcgctcc ccgcgctctc
     4621 ggcgcctccg gcggcgggcg gcgcctggcc cgcgacgtcc gcgacgcccg cgacgtctcc
     4681 cgcagccgca ccctcacccg ggcgctgctc cgcgccatcg gcgtcctcga cccgcggccc
     4741 tgacgggccg gtccggaaga cctgcgcccg taagtacccg gatcccggca ccggcaccgg
     4801 caccggcggt ggcttcggcg ccagcaccgg cagcgcctcg gcgggcagcg acgggcgcag
     4861 ggccgccagc gcctcgccca tctcctcggc gctctggtag cgctcggccg ggtccttggc
     4921 cagcgcagtg agcaccaccg cctccagctc ctccggcacg gcggggttcc gcacccgtgg
     4981 cggcgggggc ggctcgtagg cgtgctgcat cagggtcgcc atcgccgagt cggccttgaa
     5041 cggcaccttc ccggtgagca gctcgtagag caccaccccg gccgagtaca ggtcggtgcg
     5101 ggcgtcgacc ggcaggttga aggcatgctc gggagtcatg taggcggggg tgccgatgat
     5161 cgtgtcggcg gcggtgagct gctggtcgcg ctgggcgagc agcttggcga ggccatcagc
     5221 acgttgctcg gcttcacgtc ccggtggacg atgccccgcc ggtgcgcgta gcccagcccc
     5281 tccagcacgt gtccgagcag ctcggtgccg cggcgcgggg cgggcggctc ggcgaccacg
     5341 tcggccaggg tgatgccgtc ctcgacgtac tgcatcacca ggtaggcctg gccgtcctgc
     5401 tcgccgtagt cgtagacctg gatgatgttg ggatgctgca gcgccgcgat cgagcgggcc
     5461 tcccgctcga agcgggcggc gaaggccggg tcgccgggga aggcgagcac cttgacggcc
     5521 acgatgcggc cgagggacgg ctggaacgcc tggtagaccg ctgacgtgcc gccgcggcgg
     5581 attacctcgc gaagttcata tggtccgagc cgacgacctg tcaggtcgct catggtggct
     5641 ctcgcatcgc gtccacccct gccgccatcc accccaagta cccgagaagg ctacccggta
     5701 accgggttga gatggtaatt gagcattcac ccccttgaga tagacggcag gaatgaccgc
     5761 accagcggtg gcgttcgcgc tggtagatgt cgctgaggat gaggatgtgg tcgcctcgct
     5821 ggtcgatgtc gatggcggcg gcgtcgtggt ggtcgtctcc aggctggtgg tggtgacgtt
     5881 gctgctgctg gtgctgccga tggtggtgga ggtgtcattg gtggtgctgg tgtcggtggc
     5941 gccggtggtg gtctgggtgg tggtctcgct gcttgaggac gtcgaggagc tggagctggt
     6001 ctcggtcccg ctgctgctgg tcgctccgcc accggggccg atggtggtgg tggtttcgct
     6061 gccgccgggc tgcgagaccg tcgtctcggt cggcgagccg cccgagcccg aggtgtcggt
     6121 cggtccggcg gccgtggtgt cgctggccgg gggctcggtg gagggcggcg gggcgaccaa
     6181 ctgggcgccg tcgaggatca gggtgaggtt gatctcgttc ttgagcttgg cggtcacctt
     6241 gaccgagacg tccttggtcc cggcgctgcc ggtggtccgg tcgaacacgg cgctggtgcc
     6301 cttgtcgacg tcgaccagca tgacgttggc gatctgccgg ggcgcgggtg cgaccgtcac
     6361 gatggtggcg gccttgaagc ccggccaggc ggccccggag tactgcgagg tcttggtcag
     6421 gtccgtgggt tccagcagcg ggttgccgca ggcgcagcgg gaccgcggca cgcccagctt
     6481 gtcgatcagc acggcggtgc cggcctgcag gaccgcctgg aacggggtcg cgtgcccgtc
     6541 ccggtagccg ttgttggtga cccgggtgtc gagccgcagc agcaccgggg tcagctcgtt
     6601 gatgtaggcg ccgatctggg cgaccgtcag cttgttgccg ccgctccagt gcagcgtcgg
     6661 gtcgcggttg agcgcggcca cccaggcttt ggccttggtg gggttttgct gcaggaaggc
     6721 gatcatctgc gcacggttgc aggtcgactg gttctgggtg ccgccgtaga ggccgacctg
     6781 atcgccgtgg agccgcagga cgccctgcct ggcctgcgtg gccgtgccga tcgcgcccaa
     6841 cccgaagcgc ttgggcttgg agggcgtcac cgaatgggtg aacgggttcg tcccgacctg
     6901 gccgctggcc tggaggatga tctcgcgggc ctgcgcctgg ttggcgccga acagccccgc
     6961 cccccagatc gagccgccc
//
LOCUS       DASWDC010000205         4514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_945607, whole genome shotgun sequence.
ACCESSION   DASWDC010000205 DASWDC010000000
VERSION     DASWDC010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945607"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VF995_06630"
     CDS             <1..1155
                     /locus_tag="VF995_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 4"
                     /protein_id="HEX9343273.1"
                     /translation="NLAVRAQPDLRAALDGAGFVFTTFRPGGMRARHLDESIPLRHGV
                     VGQETAGPGGFLMACRSVPVLLRIAALCARVAPSAWIVNYTNPTNIVTDAVLRFGHRV
                     GAPAARIVGLCDQHAGDLERWAGLLGFPPGWALEADWIGLNHATWAERVRLRNATGDP
                     DLSAEVRRRLARLEPPAGADAATRQLAELGRALGCLPNSYARYYFFHDDVVAELRAKG
                     TTRAQEIMARLPALYADYAEAAGQDAPDPSRERGGGEHGELAVDVICAIAADEGRRLI
                     VNTRNRGAIEGLPAEAVVEVPARVDAQGPTPLVMGALPHPVRGLVAAIHEYEWLAAEA
                     AATGSRRLALQALMVHPFIRRKAAAEAILDEGLAAHRDHLPQFSRDRAGT"
     gene            complement(1163..1702)
                     /locus_tag="VF995_06635"
     CDS             complement(1163..1702)
                     /locus_tag="VF995_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX9343274.1"
                     /translation="MARARSVSVAAGAVALLGVALLAAQVVADRSGHRSRRATAATGT
                     GAPRATYLLEPSGRPAVGVSVEVAATPAARERGLMGRTSVPAGTGMVFLMPSDTTVRF
                     WMKDTLVPLSIAFVAADGHVVEVDEMPPCHANPCPTYGPNRPYRSAIELAAGGFSRAG
                     IHPGDLVVPEDPTSLPAAA"
     gene            complement(1702..2466)
                     /locus_tag="VF995_06640"
     CDS             complement(1702..2466)
                     /locus_tag="VF995_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343275.1"
                     /translation="MNLSGMRLGKQPARHDPRTLLLADYLDAEVLPAPPPHLRWTKNV
                     SSWPMMRNDTIGDCTIAAAGHCVQEWTAFSATEMIPSDDDVVAAYSAITGYDPVTGAN
                     DNGAVELDVLNHWRKLGIAGHQIMAYVATEPGNHDHVKDSVQLFGNCYIGLGLPVTAQ
                     RQRVWSVPPGGPSGDGAPWSWGGHAVPVVGYDTRGLVCVTWGALKRMTWGFWDAYCDE
                     AYAVLSRDWLKPSGEAPSGFDLAALEADLHSLDKQG"
     gene            2807..>4514
                     /locus_tag="VF995_06645"
     CDS             2807..>4514
                     /locus_tag="VF995_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX9343276.1"
                     /translation="MRLAIGAVLTFFAVALTGRRAEELVRLITVGKPAPERFDGAWTR
                     LRAELVEVFGQRKLLKRPLPGLAHFFTFWGFVLLLTTIAEAYGELFSDRFALPLIGHW
                     AVLGLLQDLIAVAVLASLLVFAAIRLRESPARRERRSRFFGSHTGQAWVILAMIAGVI
                     VTLLLYRGARAARGTLPYGGAAFASHAVGRLLDGLAASTLEALDHGFLLAHLAVVLGF
                     LVLVTYSKHLHIFTAPFNVAFSRRPDALGPLATLKIDPEEMSEEDVFGAGKIEDFKWK
                     QLLDLVTCTECGRCQDQCPAWNTGKPLSPKLVITDLRDHMFAKAPYLLGDATAEAPDG
                     HDPSVLSQDLVTDVVGSDVLWSCTTCGACVEQCPVDIEHVDTIVDMRRYQVLMESRFP
                     TEAGTMLRNIENSGDPWGLGGGQRLAWKDGLEFDVPVVTDRIPDDVEYLYWVGCAGAL
                     EDRARKTVQSTARLLHRAGVSFAVLGPRESCTGDPARRLGNEYLYQTQAQQNIGTLST
                     VGARKVIASCPHCFNTLAREYPDLGATGYEVIYHSELLQRLVAEGRLEPSSPLELTVT
                     YHD"
BASE COUNT          627 a         1638 c         1594 g          655 t
ORIGIN      
        1 aacctcgccg tgcgggccca gccggacctg cgggccgccc tggacggcgc cggcttcgtc
       61 ttcaccacct tccggccggg cgggatgcga gcccgccacc ttgacgagtc gatccccctg
      121 cgccacggcg tggtggggca ggagacggcc gggcccgggg ggttcctgat ggcctgccgc
      181 tcggtgccgg tgctgctgcg gatcgccgcg ctctgcgcgc gggtcgcccc cagcgcctgg
      241 atcgtgaact acaccaaccc caccaacatc gtcaccgacg cggtgctccg cttcggccac
      301 cgggtcggcg ccccggcggc gcgcatcgtt ggtctctgcg accagcacgc cggcgacctg
      361 gaacgctggg ccggcctgct cggcttcccg cctggatggg cgttggaagc cgactggatc
      421 gggctgaacc acgccacctg ggcggagcgg gtccgcctcc gaaatgccac cggtgacccc
      481 gacctcagcg ccgaggtgcg ccgacgcctc gcccggctgg agccgccggc gggcgccgac
      541 gcggccaccc gccagctggc cgagctggga cgggcgctcg gctgcctgcc gaactcctac
      601 gcccgctatt acttcttcca cgacgacgtg gtcgccgagc tgcgcgccaa gggcacaacc
      661 agggcgcagg agatcatggc gcgcctccca gcgctgtacg cggactacgc cgaggcggcc
      721 gggcaggacg cccccgaccc ctcgcgagag cgcggcgggg gagagcacgg ggagctcgcc
      781 gtcgacgtga tctgcgcgat cgccgcggat gaagggcgcc gcctgatcgt caacacccgc
      841 aaccgcggcg cgatcgaggg cctgcccgcc gaggcggtgg tggaggtgcc cgcgcgggtg
      901 gacgcccagg gtcccacccc gctggtgatg ggcgcgttgc cgcatccggt gcgagggctg
      961 gtcgcggcga tccacgaata cgagtggctg gcggccgagg cggcggcgac cgggagccgc
     1021 cgcctcgccc tccaggccct gatggtccac ccgttcatca ggcgcaaggc cgccgccgag
     1081 gccatcctcg acgagggcct cgccgcccac cgcgaccacc tgccgcaatt cagccgagac
     1141 cgagcgggaa catagggagg cctcaggccg ctgccgggag gctggtcggg tcctctggga
     1201 ccaccaggtc gcccgggtgg atgccggcgc ggctgaagcc gcccgcggcc agctcgatcg
     1261 cggagcggta ggggcggttt gggccgtagg tggggcaggg gttggcgtgg caggggggca
     1321 tctcgtcgac ctcgacgacg tggccgtcgg cggcgacgaa ggcgatcgac agcggcacca
     1381 gggtgtcctt catccagaag cgcaccgtcg tgtcggacgg catgaggaac accatgccgg
     1441 tcccggccgg caccgaggtc cggcccatca acccgcgttc gcgggcggcc ggggtggcgg
     1501 ccacctccac tgagaccccc accgccggcc gtccggacgg ctccagcaag taggtggccc
     1561 gcggcgcgcc ggtgccggtc gccgccgtgg cgcgccggga cctgtgcccg gagcggtccg
     1621 cgacgacctg ggccgccagc agcgcgaccc ccagcagggc gaccgccccg gccgcgacgg
     1681 acacgctgcg ggcccgcgcc atcagccctg cttgtcgagg ctatggaggt ccgcctccag
     1741 ggccgccagg tcgaatccgg agggcgcctc gccactcggc ttgagccagt cccgcgacag
     1801 cacggcgtag gcctcgtcgc agtaggcgtc ccagaatccc caggtcatgc gcttgagggc
     1861 accccaggtg acgcagacca ggccgcgcgt gtcgtagccg accaccggga cggcgtggcc
     1921 gccccacgac cagggcgccc cgtcgccgct cggcccgccg ggtgggaccg accagacccg
     1981 ctgccgctgc gcggtgacgg gcaggccgag gccgatgtag cagttgccga acagttgcac
     2041 cgagtccttg acgtggtcgt ggttgcccgg ctcggtcgcc acgtaggcca tgatctggtg
     2101 cccggcgatg ccaagcttcc gccagtggtt gaggacgtca agctcgaccg cgccgttgtc
     2161 gttggcgccg gtgacggggt cgtagccggt gatcgcggag taggcggcga ccacgtcgtc
     2221 gtcgctcggg atcatctcgg tcgcgctgaa ggcggtccac tcctggacgc aatggccggc
     2281 cgccgcaatc gtacagtcgc cgatggtgtc gttgcgcatc atcggccagc tgctgacgtt
     2341 cttcgtccag cgcaggtgcg gcgggggcgc gggcaatacc tcggcgtcga ggtagtccgc
     2401 gagcagcagg gtgcgcgggt cgtgccgggc cggctgctta ccgagccgca tgcctgagag
     2461 gttcattggg tggggcatca ttgctccatt tcgaccggta ggactggttc ggagagatca
     2521 tgtaccaatg tgacaagacc ggaaagtgtc gctgatcacc tcggggatgt gccgtccagc
     2581 accgcggacg ctatcaagca gcgacttccc ctgcttcggt tgttcacagc cccctccatc
     2641 gaagttatcc acaactttcg cgaggggccc tgacaggccc gggcgcggcg gctacgctcc
     2701 tggggctggt ccaacccgcg gccctgccag ggaagttacc gggcggtaag ctacgtggac
     2761 gaggacagcg acggatcagg cagacgacgg atcaagggag gccggcttga ggctcgccat
     2821 cggggctgtg ctcacgttct tcgccgtggc gctcaccggc cgccgcgccg aggagctggt
     2881 ccgcctcatc acggtcggca agccggcgcc cgagcgcttc gacggcgcct ggacccgcct
     2941 gcgggccgag ctggtcgagg tgttcggcca gcggaagctc ctgaagcggc cgcttcccgg
     3001 gctggcgcac ttcttcacct tctggggctt cgtgctgctg ctgaccacca tcgccgaggc
     3061 atacggcgag ctgttcagcg accggttcgc cctgccgctg atcggccact gggccgtgct
     3121 cgggctgctg caggacctga tcgcggtggc ggtgctggcg agcctgctgg tgttcgcggc
     3181 gatccgcctg cgcgagtcgc cggcccggcg ggaacgccgc tcgcgcttct tcggctccca
     3241 caccggccag gcctgggtga tcctggcgat gatcgccggg gtgatcgtca ccctgctgct
     3301 ctaccggggc gcccgggcgg cccggggcac actcccctac ggcggcgcgg cattcgcttc
     3361 gcacgcggtc gggcggctgc tcgacgggct cgccgcttcg acccttgagg ccctcgacca
     3421 cggcttcctg ctcgcccacc tggcggtcgt gcttggcttc ctggtactgg tcacctattc
     3481 caagcacctg cacatcttca ccgccccgtt caacgtcgcc ttctccaggc ggccggacgc
     3541 gctcggcccg ctggccacac tcaagatcga ccccgaggag atgagcgagg aggacgtctt
     3601 cggcgcaggc aagatcgagg acttcaagtg gaagcagctc ctcgacctcg tcacctgcac
     3661 cgagtgcggc cgctgccagg accagtgccc cgcctggaac accggcaagc cgctgtcgcc
     3721 caagctggtc atcaccgacc tgcgcgacca catgttcgcc aaggcgccct acctgctggg
     3781 cgacgccacg gccgaggcgc cggacgggca cgacccgtcg gtcctctccc aggacctggt
     3841 caccgacgtg gtgggcagcg acgtgctgtg gtcgtgcacc acctgcggtg cctgcgtcga
     3901 gcagtgcccg gtcgacatcg agcacgtcga caccatcgtc gacatgcgcc gctaccaggt
     3961 cctgatggag tcccgcttcc cgaccgaggc gggcaccatg ctgcgcaaca tcgagaactc
     4021 cggcgacccc tggggtctcg gcggcggcca gcgcctcgcc tggaaggacg gtctggagtt
     4081 cgacgtgccg gtggtcaccg accggatccc tgacgacgtc gagtacctct attgggtggg
     4141 ctgcgccggg gccctcgagg accgcgctcg caagacggtg cagtcgacgg ctcgcctgct
     4201 gcaccgggcc ggcgtcagct tcgccgtgct ggggccgcgc gagtcctgca ccggcgaccc
     4261 cgcccgccgg ctcggcaacg agtacctcta ccagacacag gcgcagcaga acatcgggac
     4321 gctgagcacg gtcggcgcgc gcaaggtgat cgcctcctgc ccccactgct tcaacacgct
     4381 ggcgcgcgag taccccgacc tcggcgccac cggctacgag gtgatctacc actccgagct
     4441 gctgcaacgg ctcgtggccg aggggcggct ggagccgtcc tcgccgctcg agctcaccgt
     4501 gacctaccac gacc
//
LOCUS       DASWDC010000206         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_193030, whole genome shotgun sequence.
ACCESSION   DASWDC010000206 DASWDC010000000
VERSION     DASWDC010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193030"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VF995_06650"
     CDS             complement(<1..793)
                     /locus_tag="VF995_06650"
                     /inference="COORDINATES: protein motif:HMM:NF037284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343277.1"
                     /translation="MTDHRPAVREVAFDGGHHALLVTAATDDDPAGLLDGLGLASGRP
                     VIVLVGGAVALAEDPLTPVCRLPGAALVRAAGMAGAAIVDGTTDADAMAAIGDAYADA
                     EADERAGGVGETAGVAGRGPLLGVAAAGQVLLPRDLPEGRGDRRVRLEANHSHYALAA
                     GDQWSAAAARLLPRLAAALAGGAPVVTVILGGGPAARAEALEAVRRGWPLFVIDGSGG
                     TADDIAQWWHTTVSHPAHPPGRARAPWRRRGPRSRDRDRDRNSELE"
     gene            complement(936..2258)
                     /locus_tag="VF995_06655"
     CDS             complement(936..2258)
                     /locus_tag="VF995_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343278.1"
                     /translation="MGEDLREQARTVLDGNWIGSSTIPSRGLYPHQWSWDAAFIAIGR
                     SWAEQRRAQEELETLPAAQWANGMVPHIVFNPAVPTEDYFPGPDFWQSERCPNAPHKL
                     RTSGITQPPLHARAALEIYQHASDRDEALAFLGRVYSKLAAQHAYLARWRDVGGHGLA
                     AIVHPWESGLDNSPLWDSELDSLVIPPGALPRYQRADLRHANAADRPTDAAYDRYVYL
                     AITYRDADYDDATLLDRSPFLVESVLFNAIYLWSVRALAEIAALLGTDPGPHREQAAR
                     LHDAILARFWDPSCGHIFGYDVRNGCPVPKDSIASMTPLLDPDLPGEVAAAIVADLSS
                     PHFTAPAAERHHLFPSYDLEAPDFDRRRYWRGPVWINTDWLVWRGLRQHGAAALAKQV
                     EHDIVALVERSGFREYFDPFGGEGYGGMDFSWTAALLLDVLASRERPA"
     gene            complement(2281..2700)
                     /locus_tag="VF995_06660"
     CDS             complement(2281..2700)
                     /locus_tag="VF995_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003961979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HEX9343279.1"
                     /translation="MASQGRTHSGRGESGRSGSDGDRDPAAVRPYALTGGRTRPGQLD
                     LDLAIETLVSTTAQGEAAWPHLTPEGRKIVVLCRDLVSVAEISARLEVPLGVAQVLIA
                     DLAVEGRVRLHPPRRPGSGNRPDLALLERVLTGLRSL"
     gene            complement(2675..>3163)
                     /locus_tag="VF995_06665"
     CDS             complement(2675..>3163)
                     /locus_tag="VF995_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343280.1"
                     /translation="VPLRRHRAVPPGDQAPAAPGPQRQPQRPPVAAPVATNTAASEEE
                     AAEVPEIILDLPPAPDQGAAVVLTEAGLPRRPMTKLRSGVEVRLPAAPPPGSGSAPPA
                     VELELETDLRARSPEGVRSMLNSYQAGLQRGRRAAATTRTATRNGAPPGSRSDGQPGS
                     HP"
BASE COUNT          383 a         1118 c         1249 g          413 t
ORIGIN      
        1 cctccagctc ggaattccgg tcccggtccc ggtcgcggct ccggggcccg cggcggcgcc
       61 agggggcgcg cgcgcggccc ggggggtggg ccgggtggct gacggtggtg tgccaccact
      121 gggcgatgtc gtcggcggtg ccgccgctgc cgtcgatcac gaacagcggc cagccgcgcc
      181 gtaccgcctc cagcgcctcg gcgcgggcag ccggcccgcc gccgaggatc accgtcacca
      241 cgggagcgcc gccggccagc gcggcggcga ggcgtggcag cagccgcgcc gccgccgcgc
      301 tccactggtc gccggcggcc agcgcgtagt gggagtggtt ggcctccagt cggacccggc
      361 ggtcgccgcg cccctcggga aggtcgcggg gcagcagcac ctgcccggcg gctgcgaccc
      421 cgagcagcgg cccacggccg gcgacgcccg cggtctcgcc gacgcctccg gcccgctcgt
      481 cggcctcggc gtccgcgtag gcgtcgccga tcgcggccat ggcgtcggcg tcggtggtgc
      541 cgtcgacgat cgcggcgccg gccatgccgg ccgcccgcac cagcgcggcg ccgggcaggc
      601 ggcacacggg ggtgagcggg tcctccgcca gcgccacggc gccgccgacc agcacgatca
      661 ccggccggcc gctcgccagg ccgagcccgt cgaggagccc ggcgggatcg tcgtcggtgg
      721 cggcggtgac gagcagggca tgatggccgc cgtcgaaggc gacctcgcgc acggctggcc
      781 ggtgatcggt catcgcttcg tcatgccggg aaaccctcca ccctcaccct ccacctctga
      841 caacgcctgc ttctgcggcg cctaatctaa atgcgcgaac cctcccgcct gcaattcgtg
      901 cagctccgca ggagggccgc tgccgcagaa cgcgcctaag caggccgttc gcggctggcc
      961 agcacgtcga gcagcagggc ggcggtccag ctgaagtcca tgccgccgta gccctctcca
     1021 ccgaaggggt cgaagtactc ccggaagccg gagcgttcca ccagcgcgac gatgtcgtgc
     1081 tcgacctgct tggccagcgc agccgcgccg tgctggcgca gcccgcgcca gaccagccag
     1141 tcggtgttga tccagacggg cccgcgccag tagcggcgcc ggtcgaagtc gggcgcctcc
     1201 aggtcgtagc tcgggaacag gtggtgccgc tcggcggcgg gggcggtgaa gtgcggcgag
     1261 ctgaggtcgg cgacgatcgc ggcggcgacc tcgcctggca ggtcggggtc gagcaacggc
     1321 gtcatcgagg cgatgctgtc cttggggacg gggcagccgt tgcggacgtc gtagccgaag
     1381 atgtggccgc aactcgggtc ccagaagcgt gccaggatcg cgtcgtggag gcgggcggcc
     1441 tgctcgcggt gcgggccggg gtcggtgccg agcagggcgg cgatctcggc cagcgcccgc
     1501 accgaccaca ggtagatggc gttgaacagc accgactcga ccaggaaggg gctgcggtcg
     1561 agcagggtgg cgtcatcgta gtcggcgtcg cggtaggtga tcgccagata cacgtagcgg
     1621 tcgtaggcgg cgtcggtggg ccggtcggcg gcgttggcat gccgcaggtc ggcgcgctgg
     1681 tagcgcggca gcgccccggg gggaatcacc agcgagtcga gctcgctgtc ccacagcggc
     1741 gagttgtcga gccccgactc ccacgggtgc acgatcgcgg cgaggccgtg gccgcccacg
     1801 tcacgccagc gggccaggta ggcgtgctgg gcggccaact tggagtagac ccggccgagg
     1861 aacgcgagcg cctcgtcccg gtcgctggcg tgctggtaga tctccagcgc cgcgcgggcg
     1921 tgcagcggcg gctgggtgat gccggaggtg cggagcttgt ggggcgcgtt ggggcagcgt
     1981 tcgctctgcc agaagtccgg cccggggaag tagtcctcgg tcggcaccgc cgggttgaag
     2041 acgatatggg gaaccatgcc gttggcccac tgcgccgccg gcagcgtctc cagctcctcc
     2101 tgggcgcgtc gctgctcggc ccaggagcgg ccgatcgcga tgaaggccgc gtcccagctc
     2161 cactggtgcg ggtagaggcc cctggacggg atcgtcgagc tgccgatcca gttgccgtcc
     2221 agcaccgtcc gtgcctgttc gcgcaggtcc tcgcccatgc cccaagtgta gggggtcagc
     2281 tcagaggctg cggaggcctg tcaggacccg ttcgagcagg gcgaggtcgg ggcggttgcc
     2341 gctgccgggt ctgcggggcg ggtggagccg gaccctgccc tccaccgcca ggtcggcgat
     2401 caggacctgg gcgacgccga gggggacctc cagccgggcg gagatctcgg cgaccgacac
     2461 caggtcccgg cacagcacca cgatcttgcg gccctccggg gtgaggtgcg gccaggccgc
     2521 ctcgccctgc gcggtggtgg agaccagcgt ctcgattgcc aggtcgaggt cgagctggcc
     2581 ggggcgggtg cgcccaccgg tcagggcgta ggggcggacc gccgccgggt cgcggtcgcc
     2641 atcggagccg ctccggccgc tctcgccccg gccgctatgg gtgcgaccct ggctggccat
     2701 cgctcctcga tcctggcggg gcgccgttgc gggtggcggt cctggtggtg gccgccgcgc
     2761 gccgcccgcg ttgcaggccg gcctggtagc tgttcagcat gctacgcacg ccttcgggtg
     2821 agcgggcacg caggtcggtc tccagctcga gctccaccgc cggtggagcc gagccggagc
     2881 cgggtggcgg cgcggcgggg agccggacct ccacccccga tcgcagcttc gtcatcggcc
     2941 ggcgggggag gcccgcctcg gtcagcacca ccgccgcacc ctgatccggg gcgggcggca
     3001 ggtcgaggat gatctcggga acctcggcag cctcctcctc ggaggcggcc gtgttggtgg
     3061 ccaccggcgc ggccacggga ggccgctgcg gttgccgctg cgggcctggc gcggcgggag
     3121 cctggtcgcc gggcgggacc gcgcggtggc ggcgcagggg gac
//
LOCUS       DASWDC010000207        12502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_552519, whole genome shotgun sequence.
ACCESSION   DASWDC010000207 DASWDC010000000
VERSION     DASWDC010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12502
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552519"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VF995_06670"
     CDS             complement(<1..1224)
                     /locus_tag="VF995_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343281.1"
                     /translation="MGVAGLVVAALLLGLQFVRLGRNHLGSGPTAPFVVGTSWQLDEE
                     LAARHIRVALTPGNGYDGQWFLGLGYDPLLRHGLANGFDMPRYRARRPLLPLVGWVLG
                     VGQPVAVPVALLAVEVLAVALGCAAAARILGASGWSRWWGVGFAVIPGVAVGVMFGTA
                     EPLGLALATLGLCLVLEGRIAAAAAAFAAAGLTKETYLGFAAAAAVWLLLRPGDVRTR
                     VRTALTVATPGVAALALWWWYVAWTVPASGSDTAGDQAFSLPLVGWAHTLARIASGRY
                     VPDAPVGPAGPALLIGTFVLLLAAVAAGPRLLRGRRRPDGEATVEATSEGAGTGTGAR
                     GWTLAAWTGLLMGCYGLALAGTLLDRFLSASRALAPAVLAAGLSLAASLAASPSHHPP
                     EPGPGPGPGQAASVHP"
     gene            complement(1261..1959)
                     /locus_tag="VF995_06675"
     CDS             complement(1261..1959)
                     /locus_tag="VF995_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013475422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9343282.1"
                     /translation="MVDGTVLVTGGTGGLGVAVTEELAAAGWRVVATALPRQAEIAPA
                     ETRDGVEVVGADLTDPNLVDVVIGVATGRPRSPLRAVVNLVGGYAGGPRVHETPVEDF
                     ERQLTLNLRPAFLVSRAALPHLVEAGGGSVVYVSSRAAVQPFAGAAGYITAKAAVLAF
                     AQVVAVEYRTQGVRANTVLPSVIDTPANRTAQPQADHSKWVPPAEIARVIRFLCGEES
                     APTSGAAIPVYGRA"
     gene            2126..2923
                     /locus_tag="VF995_06680"
     CDS             2126..2923
                     /locus_tag="VF995_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009192999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX9343283.1"
                     /translation="MRAALFVTCFNDTVFPATGKAVVRLLERLGHEVDFPVEQTCCGQ
                     LHFNTGYQHETVPLLRRFVRVFGDYEAVVAPSGSCAAMVRDYYPRVAELAADEPLAAA
                     VAALVPRVHELSEFLVGVLGVIDVGAYYPHRVTYHPTCHSLRLLKVGDAPLRLLRAVR
                     GIDLVELPAAAECCGFGGTFAIKNADTSMAILADKVRNVLDTRAEVCTAGDNSCLAHI
                     GGALSRQRTGVRTVHLAEILASTEDDADPDGAAAAVQVEAEVGEGTG"
     gene            2923..4470
                     /locus_tag="VF995_06685"
     CDS             2923..4470
                     /locus_tag="VF995_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEX9343284.1"
                     /translation="MAGAGFSGVPFPKAARQALADTQLRRNLGHATATIRRKRADVVG
                     ELPDWEALREAGRAIKQRVLRHLDSYLEELEESVRRAGGLVHWARDAAECNQIVTEIA
                     HAHGATEVVKVKSLTTDETGLNEALAAAGVSAIETDLAELIVQLGDDRPSHILVPAIH
                     KNRAEIRELFRRTLDVDTGGLSDQPAELAGAARRHLRRKFLSAKVAVSGVNFAIADTG
                     AVCVVESEGNGRMCLTLPEVLVSVMGIEKLLPTWQDLEVFLQLLPRSSTGERMNPYTS
                     FWAGVRDGDGPREFHLVLLDNGRTKVLADQIGRQTLHCIRCSACLNICPVYERTGGHA
                     YGSVYPGPIGAILTPQLVGVERAGSLPYASSLCGACYEVCPVKINIPEVLVHLRARVV
                     AEHRAERRLPRPEELSMETLAWIFADRRRYELAQRLARLGQRPWARSGRIRRLPAALG
                     GWTEGRDAPALPAQTFREWWRERERDRAREREKQREGEQVRQRISHGDSHGNDSSRHD
                     SGGKEPE"
     gene            4467..5126
                     /locus_tag="VF995_06690"
     CDS             4467..5126
                     /locus_tag="VF995_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEX9343285.1"
                     /translation="MIAREEVLARIREALDDVPDGERPEDVLVERAYRQHDDASASER
                     VARFAERVADYKAAVQWVSPEELPAALMRALHARGVERLVVPPDLPAAWRPDGTELLV
                     DGELANDVLDAADGVLTGCALGIAETGTIVLDGGARQGRRAITLLPDYHLCVVEAAQI
                     VGSVPEAVAALDGAARERRPITFVSGPSATSDIELSRVEGVHGPRTLEVVIVGEAEAS
                     H"
     gene            5126..5539
                     /locus_tag="VF995_06695"
     CDS             5126..5539
                     /locus_tag="VF995_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX9343286.1"
                     /translation="MPTVDEARAFVAANHQAVLITRRSSGSVQSSPVTVGIDGDGKVV
                     ISSRATAYKVRNLRRDPHAVLCVFTDNFFGPWLQVEGAAELVSLPEAMDGLVDYYRRI
                     SGEHPDWDDYRRAMVRDRRLLIRVSIDQVGPTRHG"
     gene            complement(5504..7087)
                     /gene="lnt"
                     /locus_tag="VF995_06700"
     CDS             complement(5504..7087)
                     /gene="lnt"
                     /locus_tag="VF995_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX9343287.1"
                     /translation="MERGRWHRWRGRLLAAALGMPLALAFPRPALWWCAFVGLVPLLL
                     WARAAPTGREAAMRGWCGGIGFFLAVDSWLLPNTGVFTAPAALFLGALWLPLGWLGWR
                     LLRDPLSAGRLAAAVLLLPAVWVLGEVVRSWSALGGPWALLGASQWNDRPVLAVAAIG
                     GVWAVSFLLVAVNVAVAAALAPRPRGGPGLAGLGLAGRAGAVVVAAVLVGAAAAYGAT
                     RPAPPAAGSVRVALVQPGIIHQVEPRFEASETASLALGAQHPDLIVWSESSIGRDPAA
                     NPGDVARLERIVRATGADVLANVDARRGTGTGTGTGGGIYKNSLLIGPDGPLGSYDKM
                     RLVPFGEYVPLRPLLGWVTHFTKAATENRHHGQHLVVLHAATSSGPLALGPLICFESS
                     FPDLGRGLANLGADLIVIQSATTTFQGTWGPDQHASLAAVRAVESGRPVVQASISGVS
                     AAFDPSGRRLAWYPQSWRGAAVVTMPLARATTLYDRWGEWVPAASALIVVVAGVAAVR
                     RRVPWRGASAVPGRADLVD"
     gene            complement(7148..8614)
                     /locus_tag="VF995_06705"
     CDS             complement(7148..8614)
                     /locus_tag="VF995_06705"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005447437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX9343288.1"
                     /translation="MSIATSERSVVEAVNGQLYIGGEWRPASGGRTMPVEDPATGEVL
                     REVADASPTDAAAALDAAVAAQEEWAAWPPRERGEILRRAFETIMARSDQLALLMTLE
                     MGKSVAESKAEIAYAAEFFRWFSEEAVRIHGRYTVAPNGKGRLLTMKQPVGPCLLITP
                     WNFPMGMGTRKIGPAVAAGCTMVMKPAGLTPLSMLALTQILEEVGLPPGVLNVITTSR
                     SGATTGPLIADPRLRKLSFTGSTEVGRKLLAQAADQVLRVSMELGGNAPFLIFEDADL
                     DAAVEGAMLAKMRNIGEACTSANRFHVAAPVAEEFAGRLGERMGALKVGRGTDDGVQV
                     GPLIERAAVDKVSALVGDALDRGAKAVVGGRAIDGRGYFYQPTVLAGVPAGARVLREE
                     IFGPVAPVSSFDSEEAAVAAANDTEFGLVAYVYTSDLKRAFRVVEALQSGMVGLNQGI
                     VSNPAAPFGGVKQSGIGREGGPEGIDEYLDVKYVAMNL"
     gene            8794..10122
                     /locus_tag="VF995_06710"
     CDS             8794..10122
                     /locus_tag="VF995_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEX9343289.1"
                     /translation="MTVHQAQAGIRPPARPPAERVWRVLGAPLDPRTWMATGYLLLSM
                     FFGIAWFAVIITLFLLSIGLAVVWVGVPLFALKIVCLREIARVERAWARTALGADIPD
                     PYRPPERGSLWRRLRGMATDPATWKDFLYVMVLLPLGTLWFSIVTVVWSVAFSLLAVP
                     VWYLWLPGHRASFFEINGHANLVIGTLVEALALGLAGLLLCVVAAWVSRGLGAGHAAI
                     ARGLLGPSPQQLARRVEALQASRARTVDAAEAERRRIERDLHDGAQQRLVALAMDLGM
                     AKERLAGDADPEAVRALVAEAHDEAKRALVELRDLARGIHPAVLADRGLDAAISALAA
                     RSPVPVEVEVDTERLASPVESIAYFVVAEALTNVAKHARATEVTVRISRERERLLVEI
                     RDNGAGGATVAHGGGLGGLADRVAGIDGRLVVDSPPGGPTVVRAELPCGS"
     gene            10110..10754
                     /locus_tag="VF995_06715"
     CDS             10110..10754
                     /locus_tag="VF995_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9343290.1"
                     /translation="MRVVIAEDSVLLREGLARLLGEGGHEVVAKVGDGPGLVQAVDAW
                     RPDIAIVDVRMPPTHSDEGLRAAVEVRERAPRTAILVLSQYVEERYAAELIGASAEGI
                     GYLLKDRVADVGEFLAAVRRVGEGGAAIDPEVVAQLLGRRRCDKPLAQLSPRELEVLA
                     LMAEGRSNAGIAERLVVTEGAVEKHISSIFAKLGLPAAEGDHRRVLAVLTYLRG"
     gene            10818..11231
                     /locus_tag="VF995_06720"
     CDS             10818..11231
                     /locus_tag="VF995_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343291.1"
                     /translation="MPAGTDGTHGTHGDGMDWPEQHRTTWIIGAASNAALLAALGLLL
                     SPASKRGVATVMVVVGLGAGIGAVLAGVLPPRLRQLTVMERLNNAAFTLVAAVAALIA
                     SQLAEPWTVIIPGLVGGLLIGRALRLERPKPKSDS"
     gene            complement(11242..12180)
                     /locus_tag="VF995_06725"
     CDS             complement(11242..12180)
                     /locus_tag="VF995_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343292.1"
                     /translation="MALLILPHQAGATGGQATDVATQLKQLKAALADAKASQGSLQKA
                     LNEADAALNQAENELAAANADQLAATAEAKQAVKLMASSKVQVARLRQVVGDQARDIY
                     INGVPASLEVLVRNDNVQQLLSQVTILDHLARQNHDDLSDMLIAQKEYAAARVALVQA
                     RKDAQQAAGQIQHKVGQAQQLRDLRTKAMAALLAKVSQIAGKSAALRLQQQIASQQPV
                     SVPTGSGHCDLSGASASEVWIIMHESGDNPFAQNPHSTAFGLGQLLLDLRIRLLGANF
                     DTTNCALQLQAFRGYVKERYGTAEAAQAFWEAHHWY"
BASE COUNT         1745 a         4747 c         4407 g         1603 t
ORIGIN      
        1 agggtggaca ctggccgcct gcccgggccc gggcccgggc ccgggctcgg gcggatggtg
       61 ggagggcgac gccgccaggc tcgcggccag cgacagcccc gccgcgagca cggccggcgc
      121 cagcgcccgc gacgccgaca ggaaccggtc cagcagcgtg cccgccagcg ccagcccgta
      181 gcagcccatc agcagcccgg tccaggcggc cagcgtccag ccccgcgctc cggtaccggt
      241 acccgcgccc tcgctcgtgg cctcgacggt cgcctcgcca tcgggccggc gccggccgcg
      301 cagcagccgc ggtcccgccg ccaccgcggc cagcagcagc acgaaggtcc cgatcagcag
      361 cgccggcccg gcaggcccca ccggcgcgtc gggcacgtag cggccgctcg cgatccgtgc
      421 cagggtgtgc gcccagccca ccagcggcag cgagaacgcc tggtcgccgg cggtgtcgct
      481 gccgctggcc ggtaccgtcc aggcgacgta ccaccaccac agcgcgaggg cggccacgcc
      541 cggggtggcc accgtcagcg ccgtccgcac ccgcgtccgg acgtcaccgg gccgcagcag
      601 cagccacacg gccgccgccg ccgcgaaacc gaggtaggtc tccttggtca gccccgccgc
      661 cgcgaacgcc gccgccgcgg cggcgatccg cccctccagg accaggcaca gcccaagggt
      721 cgccagggcg aggccgagcg gctcggcagt gccaaacatc acccccacgg cgacgccggg
      781 gatgaccgcg aacccgaccc cccaccagcg cgaccatccc gaggcgccga ggatccgcgc
      841 ggccgccgcg cagccgagcg cgacggcgag cacctcgacc gccagcagcg ccaccggcac
      901 cgccaccggc tggcccacgc caagcaccca gcccaccagc ggcagcagcg gccgccgcgc
      961 gcggtagcga ggcatgtcga acccgttggc gagcccgtgg cgcagcagcg ggtcgtagcc
     1021 gagcccgagg aaccactgcc cgtcgtagcc gttgcccggc gtcagcgcca cgcggatgtg
     1081 ccgggcggcc agctcctcgt ccagctgcca gctggtcccc accacgaacg gcgcggtcgg
     1141 cccgctcccg aggtggttgc gccccagccg cacgaactgc agcccgagca gcagcgcggc
     1201 gaccaccagc ccggcgaccc ccacggggag gaccctgcgc cagggaacgg gagccaccgc
     1261 ctacgcccgc ccgtagaccg ggatcgcggc gccgctggtc ggggcggact cctcgccgca
     1321 gaggaagcgg atcacccggg cgatctcggc gggcggcacc cacttggagt gatcggcctg
     1381 gggctgcgcc gtccggttgg ccggggtgtc gatcacgctg ggaagcacgg tgttggcccg
     1441 cacgccctgg gtgcggtact cgaccgcgac cacctgggcg aaggccagca cggcggcctt
     1501 ggcggtgatg tagccggcgg ctccggcgaa cggctgcacg gcggcgcggg aggagacata
     1561 caccaccgag ccaccaccgg cctcgaccag gtggggcagc gcggccctgg agaccaggaa
     1621 cgccggtcgg aggttcaggg tgagctgccg ctcgaagtcc tcgaccgggg tctcgtgcac
     1681 cctgggcccg ccggcgtacc caccgaccag gttgaccacc gctcgcagcg gcgagcgggg
     1741 gcggccggtc gcgacgccga tcaccacgtc gaccaggttg gggtcggtca ggtcggcgcc
     1801 gaccacctcc acaccgtccc gagtctccgc aggcgcgatc tcggcttgcc tgggcagcgc
     1861 ggtcgcgacg acccgccagc ccgccgcggc cagctcctcg gtcaccgcca caccaagccc
     1921 gccggtgcca ccggtcacca gcaccgtccc atccaccatg aggcaggcgt cctttcgctt
     1981 cgccgggcgc gtctcgctgg acgcgtcgca ctggctcgcc gcggcggcaa gtctcccacg
     2041 agtgcgcggc gtccgcgcac ttgacgccta tgacgccggt gcgacggttg acgacggggc
     2101 gtgaacgcgg caaaactctg gcgacgtgcg tgccgccttg ttcgtgacct gcttcaacga
     2161 caccgtgttc cccgccaccg ggaaagcggt cgtacgactg ttggagcggc tcggccacga
     2221 ggtcgacttc ccggtggagc agacctgctg cggccagctg cacttcaaca ccggctacca
     2281 gcacgagacg gtcccgctgc tgcgccgctt cgtccgtgtc ttcggcgact acgaggcggt
     2341 ggtcgcgccg tcggggtcct gcgcggcgat ggtgcgcgac tattaccccc gcgtcgcgga
     2401 actggccgcc gacgagccgc tggccgccgc cgtcgccgcg ctggtcccgc gcgtccacga
     2461 gctgtcggag ttcctggtcg gggtccttgg cgtcatcgac gtcggtgcct actaccccca
     2521 ccgggtcacc taccacccga cctgccactc gctgcggctg ctcaaggtcg gcgacgcgcc
     2581 gctgcggttg ctgcgcgcgg tgcgcggcat cgacctggtt gagctgcccg ccgctgccga
     2641 gtgctgcggg ttcggcggca ccttcgcgat caagaacgcc gacacctcca tggcgatcct
     2701 ggccgacaag gtccgcaacg tgctcgacac ccgcgcggag gtctgcaccg ccggggacaa
     2761 ctcctgcctc gcccacatcg gcggggcgct gtcgcgccag cggaccggcg tgcgcaccgt
     2821 ccacctcgcc gagatcctcg cgtcgaccga ggacgacgcc gaccccgacg gtgcggcggc
     2881 tgcggttcag gtcgaggccg aggtcgggga agggacgggc tgatggcggg cgcgggattc
     2941 tccggggtgc cgttccccaa ggcggcccgt caggcgctgg ccgacacgca gctgcgccgc
     3001 aacctcggcc atgccaccgc caccatccgc cgcaagcgcg ccgacgtggt cggggagctg
     3061 cccgactggg aggctctgcg agaggccggc cgcgccatca agcagcgggt cctgcgccac
     3121 ctggacagct acctggagga gcttgaggag agcgtgcggc gcgccggcgg gctggtccac
     3181 tgggcacgcg acgccgccga gtgcaaccag atcgtcaccg aaatcgccca tgcgcacggc
     3241 gccaccgagg tggtcaaggt caagtcgctc accaccgacg agaccggcct gaacgaggcg
     3301 ctcgccgccg ccggggtcag cgcgatcgag accgacctgg cggaactgat cgtccagctc
     3361 ggcgacgacc gcccttccca catcctggtc ccggcgatcc acaagaaccg cgcggagatc
     3421 cgcgagctgt tccgccgcac cctcgacgtg gacactggcg gcctctcgga ccagccggcg
     3481 gagctggccg gcgcggcccg ccgccacctg cgccgcaagt tcctcagcgc caaggtcgcg
     3541 gtcagcggcg tgaacttcgc catcgccgac accggcgcgg tctgcgtagt ggagagcgag
     3601 ggcaacgggc gcatgtgcct gaccctgccc gaggtgctgg tgagcgtgat ggggatcgag
     3661 aagctgctgc ccacctggca ggacctggag gtgttcctgc agctgctgcc acgctcgtcc
     3721 actggcgagc ggatgaaccc ctacacctcc ttctgggccg gggtgcgcga cggcgacggg
     3781 ccacgcgagt tccacttggt gctgctcgac aacggccgca ccaaggtgct cgccgaccag
     3841 atcggccgcc agacgctgca ctgcatccgc tgctcggcct gcctcaatat ctgcccggtc
     3901 tacgagcgca ccggcgggca cgcctacggg tcggtctacc cggggccgat cggcgcgatc
     3961 ctcaccccgc agctggtcgg ggtggagcgg gcgggctcac tgccgtacgc ctccagcctc
     4021 tgcggcgcct gctacgaggt ctgcccggtc aagatcaaca tccccgaggt cctggtccac
     4081 ctgcgcgccc gggtggtggc cgagcaccgc gccgagcggc ggctgccccg gcccgaggag
     4141 ctgtcgatgg agacgctcgc ctggatcttc gccgaccgcc gccgctacga gctggcgcag
     4201 cgcctcgccc gcctcggcca gcggccgtgg gcccgctcgg gccggatccg gcgtctcccc
     4261 gcggcgctcg gcggctggac cgagggccgc gacgccccgg cgctgccggc gcagaccttc
     4321 cgggaatggt ggcgggaacg ggagcgggat cgggcgcgcg agcgtgagaa gcagcgcgaa
     4381 ggggaacagg tccggcagcg gatcagccac ggcgacagcc acggcaacga cagcagcagg
     4441 cacgattcgg gggggaagga gccggagtga tagcgcgaga agaggtgctg gcccggatcc
     4501 gggaggcgct tgacgacgtc ccggacggcg aacgtcccga ggacgtgctg gtggagcgcg
     4561 cctaccggca gcacgacgac gcatcggcga gcgaacgggt ggcacgcttc gccgagaggg
     4621 tcgccgacta caaggccgcc gtccagtggg tctcccccga ggagctgccc gccgcgctca
     4681 tgcgggcgct gcacgcccgc ggcgtggagc ggctggtggt gccgcccgac ctgcccgccg
     4741 cgtggcggcc ggacggcacc gagctgctgg tcgacgggga gctggccaac gacgtcctcg
     4801 acgccgccga cggggtgctg accggctgcg ccctcggcat cgccgagacc ggcacgatcg
     4861 tgctggacgg cggcgcccgc cagggccgcc gcgccatcac cctgctgccc gactaccacc
     4921 tgtgcgtggt cgaggccgcc cagatcgtcg gctcggtccc cgaggcggtc gccgccctgg
     4981 acggcgccgc ccgcgagcgg cgccccatca ccttcgtgtc cggcccctca gccacctccg
     5041 acatcgagct gagccgggtc gagggcgtcc acggcccccg caccctggag gtcgtgatcg
     5101 tcggcgaggc cgaggcttcc cactgatgcc gaccgtggac gaggcgcggg cattcgtcgc
     5161 ggccaaccat caggccgtgc tgatcacccg ccggtccagc gggagcgtgc agtcctcgcc
     5221 ggtcaccgtt ggcatcgacg gggacggcaa ggtggtcatc agcagccgcg cgaccgccta
     5281 caaggtccgc aacctgcgcc gcgacccgca cgcggtgctg tgcgtcttca ccgacaactt
     5341 cttcgggccc tggctgcagg tcgagggcgc cgccgagctc gtctcgctgc ccgaggcgat
     5401 ggacgggctg gtcgactatt accgccgcat cagcggcgag caccccgact gggacgacta
     5461 ccgccgcgcc atggtgcggg accggcgcct gctcatccgc gtctcaatcg accaggtcgg
     5521 cccgacccgg cacggctgac gcaccacgcc acggcacacg gcgccgcacg gcggccacgc
     5581 ccgcgaccac cacgatgagc gccgaggccg ccggcaccca ctcgccccag cggtcataga
     5641 gcgtggtggc gcgcgccagc ggcatcgtca ccaccgcggc gccgcgccag ctttgcgggt
     5701 accaggcgag gcggcggccg ctcgggtcga aggccgccga gacgccactg atcgacgcct
     5761 gcaccaccgg acgccccgac tcgaccgccc gcaccgccgc caggctggcg tgctggtcgg
     5821 ggccccaggt gccctggaag gtggtggtgg cgctctggat cacgatcagg tcggcgccga
     5881 ggttggcgag cccgcggcca aggtcgggaa acgacgactc gaagcagatc agcgggccga
     5941 gggcgagcgg cccggagctg gttgccgcgt gcaggaccac caggtgctgg ccgtggtggc
     6001 ggttctcggt ggccgccttg gtgaaatggg tgacccagcc gagcagcggc cgcaacggca
     6061 cgtactcccc gaagggcacc aggcgcatct tgtcgtagct gccgagcggc ccgtccggcc
     6121 cgatcagcag cgagttcttg tagatcccgc cgccggtgcc ggtgccggtg ccggtgccgc
     6181 gccgcgcgtc gacgttggcg agcacgtccg cgccggtcgc ccggacgatc cgctccaggc
     6241 gagccacgtc gccggggttg gccgccgggt cgcggccgat gctgctctcc gaccagacga
     6301 tcaggtccgg gtgctgggcg ccgagcgcca ggctggccgt ctcgctggcc tcgaagcgcg
     6361 gctcgacctg gtggatgatg cccggctgga ccagcgcgac ccgcacgctc ccagcggccg
     6421 gcggggcggg ccgggtggcg ccgtaggcgg ctgccgcgcc aaccagcacc gccgccacca
     6481 ccacggcccc ggccctgccg gccagcccca gccccgcgag gcctgggccg ccacgcgggc
     6541 gaggggcgag cgccgccgcc acggcgacgt tgacagcgac cagcaggaag ctcaccgccc
     6601 ataccccgcc gatcgcggcg accgccagga ccggccggtc gttccactgg ctcgcgccga
     6661 gcagcgccca gggcccgccg agcgcgctcc aggagcgcac cacctcgccg agcacccaca
     6721 ccgccggcag cagcagcacc gccgcggcga gccgcccagc cgacagcggg tcccgcagca
     6781 gcctccaccc aagccacccg agcggcagcc acagcgcccc gaggaacagc gccgccggtg
     6841 ccgtgaacac accggtgttc ggcagcagcc aggagtccac tgccaggaag aacccgatcc
     6901 cgccgcacca accccgcatg gcggcctccc gccctgtcgg cgccgcccgc gcccacagca
     6961 gcagcgggac cagcccgaca aaggcacacc accacagggc gggacgcggg aacgcgagcg
     7021 ccagcggcat gccgagggca gccgcaagca gccgtccgcg ccagcggtgc cagcgcccgc
     7081 gctccataca caaacctccg ggtgctccgg ccaggccgaa cccgtgatct caggcgagcc
     7141 cgtgatctca gaggttcatg gcgacatact tgacgtccag gtactcgtcg atcccctccg
     7201 ggccgccctc acggccgatg ccggactgct tcaccccgcc gaacggcgcc gccgggttgg
     7261 agacgattcc ctggttgagg ccgaccatcc cggactgcag cgcctccacc actcggaagg
     7321 cgcgtttcag gtcgctggtg tagacgtagg cgaccagccc gaactcggtg tcgttggcgg
     7381 ccgcgaccgc cgcctcctcc gagtcgaagg aactgaccgg cgcgaccggc ccgaagatct
     7441 cctcccgcag cacccgcgcg cccgccggta cgcccgccag caccgtcggc tggtagaagt
     7501 agccgcgccc gtcgatcgcc cggccgccga ccaccgcctt ggcgccccgg tcgagcgcgt
     7561 cgccgaccag cgcactgacc ttgtcgaccg cggcccgctc gatcagcgga ccgacctgca
     7621 cgccgtcgtc ggtgccacgg ccgaccttga gggcgcccat gcgctcgccg agccgccccg
     7681 cgaactcctc ggccaccggc gcggccacgt ggaagcggtt ggcggaggtg cacgcctcgc
     7741 cgatgttgcg catcttggcg agcatcgcgc cctcgaccgc cgcgtccagg tcggcgtcct
     7801 cgaagatcag gaacggcgcg ttgccgccca gctccatcga cacccgcagc acctggtcgg
     7861 cggcctgcgc cagcagcttg cggcccacct cggtcgagcc ggtgaaggag agcttgcgca
     7921 gccgcgggtc ggcgatcagc ggcccggtgg tcgcgcccga ccgggaggtg gtgatcacgt
     7981 tcaggacccc cggcggcagg ccgacctcct ccaggatctg ggtgagcgcc agcatcgaca
     8041 acggcgtcag gcccgccggc ttcatcacca tggtgcagcc cgccgccacc gccgggccga
     8101 tcttgcgggt gcccatcccc atcgggaagt tccacggcgt gatcagcagg cacggcccga
     8161 ccggctgctt catggtcagc aggcggcctt tgccgttggg ggcaacggtg tagcgaccgt
     8221 ggatccgcac cgcctcctcc gagaaccagc ggaagaactc ggccgcgtag gcgatctccg
     8281 ccttggactc ggcgaccgac ttgcccatct ccagggtcat cagcagcgcc agctgatcgc
     8341 tgcgggccat gatcgtctcg aacgcccgcc gcaggatctc gccccgctcc cgaggcggcc
     8401 aggccgccca ctcctcctgc gcggcgaccg cggcgtccag cgcggcggcg gcgtcggtgg
     8461 ggctcgcgtc ggcgacctcg cgcaggacct ccccggtcgc gggatcctcc accggcatgg
     8521 tcctgccgcc gctggcgggg cgccactcgc cgccgatgta gagctgcccg ttgacggcct
     8581 ccaccacgga gcgctcactg gttgcgatgc tcatcacggc cctcctgttg acctgcgcgt
     8641 ctgcgaacgc cagggtaacg cggcggcctc ccgctgtgat gttccaggta gccccacccc
     8701 gcccccgcca gttgtccgga taggcgcgca gcggcgcccg gcctagcctc gacctggcaa
     8761 gattgtcacc agcgaaccca ggagcaggca gcgatgaccg tccaccaggc acaggcaggg
     8821 atccggccgc cggcccgtcc gccagccgag cgggtgtggc gcgtcctggg cgcgccgctg
     8881 gacccacgga cctggatggc caccggctac ctgctgctca gcatgttctt cgggatcgcg
     8941 tggttcgcgg tgatcatcac gttgttcctg ctgagcattg gattggcggt cgtctgggtc
     9001 ggggtgccgc tcttcgcgct caagatcgtc tgcctgcgcg agatcgcccg ggtcgaacgg
     9061 gcctgggcgc gcaccgctct cggcgccgac atccccgatc cctaccggcc gccggagcgc
     9121 gggtcgctgt ggcgacgcct ccggggcatg gccaccgacc cggcgacctg gaaggacttc
     9181 ctctacgtga tggtgctgct gccgctcggc acgctctggt tcagcatcgt cacggtcgtc
     9241 tggagcgtcg cgttcagcct gttggccgta ccggtctggt atctctggct ccccggccat
     9301 cgcgccagct tcttcgagat caacggccat gccaacctgg tgatcggcac cctggtcgag
     9361 gccctggcgc tcggcttggc cggcctgctg ctctgcgtcg tggccgcctg ggtctcccgc
     9421 ggcctcggcg ccgggcacgc cgcgatcgcc cgggggctgc tcggcccaag cccccagcag
     9481 ctcgcccggc gggtcgaggc gctgcaggcc agccgcgccc gcaccgtcga cgccgccgag
     9541 gcggagcgcc gccgcatcga acgcgacctg cacgacggcg cgcagcagcg gctggtcgcc
     9601 ctcgccatgg acctcggcat ggccaaggag cggctcgccg gcgacgccga ccccgaggcg
     9661 gtccgcgcgc tggtcgccga agcccacgac gaggccaagc gcgccctcgt ggagctgcgg
     9721 gacctcgccc gcgggatcca tccggcggtg ctcgccgacc gcggcctcga cgcggccatc
     9781 tcggcgctgg cggcccgctc gccggtcccg gtcgaggtcg aggtcgacac cgagcggctg
     9841 gccagcccgg tcgagtcgat cgcctacttc gtggtcgccg aggcgctcac caacgtcgcc
     9901 aagcacgccc gcgccaccga ggtgaccgtg cggatcagcc gggagcgcga gcggctgctt
     9961 gtcgagatcc gcgacaacgg cgccggcggc gccaccgtgg ctcatggcgg cgggctcggc
    10021 ggcctcgccg accgggtcgc cgggatcgac gggcgcctgg tcgtggacag ccccccgggc
    10081 ggcccgaccg tggtgcgagc ggagctgccg tgcgggtcgt gatcgccgag gactcggtgc
    10141 tgctgcggga gggcctcgcc cgcctgctcg gcgagggcgg ccacgaggtg gtcgccaagg
    10201 tcggcgacgg gcccggcctg gtccaggcgg tcgacgcgtg gcggcccgac atcgccatcg
    10261 tcgacgtccg catgccgccc acccacagcg acgagggcct gcgcgccgcc gtcgaggtcc
    10321 gcgaacgcgc ccccaggacc gccatcctgg tgctgtcgca atatgtcgag gagcgctacg
    10381 ccgccgagct gatcggcgcc agcgccgaag ggatcggcta cctgctcaag gaccgggtcg
    10441 ccgacgtcgg cgagttcctc gccgcggtcc gccgggtggg ggagggcggc gccgcgatcg
    10501 accccgaggt ggtcgcccag ctcctcggcc ggcgccgctg cgacaagccg ctggcgcagc
    10561 tcagcccccg cgagctggag gtgctcgcgc tcatggcgga gggccgcagc aacgccggca
    10621 tcgccgaacg cctggtggtc accgaaggcg ccgtcgagaa gcacatctcc agcatcttcg
    10681 ccaagctcgg cctccccgcc gccgaaggcg accaccgccg cgtcctggcg gtgctcacct
    10741 acctgcgcgg ctgacccagc gggcgagtag gctacgggcg atcccgacac atgcggaggc
    10801 ggagggtgag cggcacgatg ccagcaggca cggatggtac ccacggcacg cacggcgacg
    10861 gcatggactg gccagagcag caccgcacca cctggatcat cggtgcggcc agcaacgcgg
    10921 ccctgctcgc cgccctgggg ctactgctca gccccgcctc caagcgcggc gtcgccaccg
    10981 tcatggtggt cgtcggcctc ggcgccggaa tcggcgcggt cctggccggg gtgctcccgc
    11041 ccaggctgcg gcagctcacc gtgatggagc gcctcaacaa cgccgccttc accctggtcg
    11101 ccgcggtcgc cgcgctgatc gcctcccagc tggcggaacc atggacggtg atcatccccg
    11161 gcctggtcgg cggcctgctg atcggccgcg ccctgcgcct ggagcgcccc aagcccaagt
    11221 cagacagctg acccctggcg ctcagtacca gtggtgcgcc tcccagaacg cctgggcggc
    11281 ctcggcggtg ccgtagcgct ccttgacgta gccgcggaag gcctggagct gcagggcgca
    11341 gttggtggtg tcgaagttcg cgccgagcag gcggatacgc aggtcgagca ggagctgccc
    11401 gagaccgaac gcggtcgagt gggggttctg ggcgaagggg ttgtcgccgg actcgtgcat
    11461 gatgatccag acttccgacg ctgatgcccc cgacaggtcg cagtgcccgc tgcccgtcgg
    11521 cacgctcaca ggctgctggg acgcgatctg ctgctggagg cgcaacgcgg ccgacttccc
    11581 ggcgatctgg ctgaccttgg ccagcagcgc cgccatcgcc ttggtgcgca ggtcgcggag
    11641 ctgctgcgcc tggccgacct tgtgctggat ctggccggcg gcctgctggg cgtccttgcg
    11701 cgcctggacg agcgccaccc tggccgcggc gtactccttc tgcgcgatca gcatgtcgct
    11761 caggtcgtcg tggttctgcc tggccaggtg gtcgaggatg gtgacctggc tgagcagctg
    11821 ctggacattg tcgtttcgga ccagcacctc gaggctggcg ggaacgccgt tgatgtagat
    11881 gtcgcgggcc tggtcaccga ccacctggcg cagccgcgcg acctgaacct tggacgacgc
    11941 catcaacttg accgcctgct tggcctccgc cgtggcggcg agctggtcgg cgttggcagc
    12001 ggccagctcg ttctcggcct ggttcagggc ggcgtcggcc tcgttcaggg ccttctgcaa
    12061 ggatccctgg ctggccttgg catccgccag ggcggctttg agctgcttca gctgggtggc
    12121 gacgtcggtc gcctgcccgc cggtggcgcc ggcctgatgg ggaaggatca gcagtgccat
    12181 cacgagggcg cccaagatcc acaggaccca ggaacgccgt tgggtcgtcg tagccatctg
    12241 ctggacgaga actcccggta ggggtcgggg gcacgtacct tgaaccttgg agccttcatg
    12301 gcgttgtggg ggtcaagcag ccatgaagcg ggtgaacaat atccagaccc cctgccgctt
    12361 gcaagcagga cgatcagctc ggacctgtct gagcggtttc accggtcacc gcatcccacc
    12421 cacttcccac ccacacgtgt cgcacggctg tggcatgctg cgcccgtgga catcgccatg
    12481 gacaacctgg aagccggcgt gg
//
LOCUS       DASWDC010000208         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_946372, whole genome shotgun sequence.
ACCESSION   DASWDC010000208 DASWDC010000000
VERSION     DASWDC010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946372"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..992
                     /locus_tag="VF995_06730"
     CDS             228..992
                     /locus_tag="VF995_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9343293.1"
                     /translation="MGRVIALANQKGGVGKTTTAINLGAALAEQGQRVLLVDMDPQGA
                     LSVGLGLSPMALEQTVYNLLMNPAADATPAIRTTKVAGLDLLPSNIDLAAAEVMLVAE
                     VAREQALARVLVPLRQHYDHVLIDCPPSLGLLTINALTAADGVLIPLECEYFALRGMA
                     LLMDTITKIRERQLNPDLEIVGILATMYDARTVHGREVLGRVADAFGDLVFTTVIAKT
                     IRFAEAPVAGQPILTYASDSAGAAAYRDLAKEVAAA"
     gene            1172..1396
                     /locus_tag="VF995_06735"
                     /pseudo
     CDS             1172..1396
                     /locus_tag="VF995_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018959660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1413..2732
                     /locus_tag="VF995_06740"
     CDS             1413..2732
                     /locus_tag="VF995_06740"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX9343294.1"
                     /translation="MSRMRAVDLIARKRDGGEIARADLRALILGYASGAVPDYQMAAF
                     LMAGYLRGFTRREAEALTEAMVASGSTLDLGKLQGRTVDKHSTGGVADATTLIVGPLA
                     AALGLKMLKLSGRGLGHTGGTLDKLESIPGFTVERSATELLDQVDRVGLAVAAQSADL
                     VPADKAMYALRDVTATVASTALIASSVMSKKLAGGASCVLLDVKTGSGAFMKDPRAAA
                     SLAGLCVEIGRAAGRRTAALVSDMSQPLGDLIGNAVEVREAVEVLRGERGGRLLDLCL
                     TLTGQLAALAGAAPHLDAGRERARYVLEGGHGLEKFRAFVAAQGGDPAVADDPAAVLP
                     AAPLAVPVHADAGGFLAAVDAEAVGWAAATLGAGRQRKDDPIDPAVAIELPTKIGDHV
                     EAGQPIATIRARSEADAERAEAALRAALRWSEQPPPAPPLVHQLIGP"
BASE COUNT          427 a         1098 c         1002 g          371 t
ORIGIN      
        1 tggacagcaa ggcgaacacg gagcgtggtt gaccacggag cgtggtcgga ggcgctgagc
       61 tgtccgtttg ggcctgctct gccgcggcgg ctatgatcct gacgcttcgt cgatcaggta
      121 attcagcgac tgatcgccaa agctccagat cgccccatct acacaacccc aaggagattt
      181 atcgacaaat cggctgccgt gaagccggtc agaggaggca aggccggatg ggaagggtca
      241 tcgccctcgc gaaccaaaaa ggcggcgtcg gcaagacgac caccgcgatc aacctcggcg
      301 ccgccctcgc cgagcagggc cagcgggtcc tgctggtgga catggacccc cagggggcgc
      361 tgtcggtcgg gctcgggctc agcccgatgg cccttgagca gaccgtctac aacctcctga
      421 tgaacccggc cgccgacgcc accccggcga tccgcaccac caaggtcgct ggcctcgacc
      481 tgctgccctc caacatcgac ctggccgccg ccgaggtcat gctggtcgcc gaggtcgccc
      541 gcgagcaggc gctggcgcgg gtgctggtgc cgttgcgcca gcactacgac cacgtgctga
      601 tcgactgccc cccaagcctt ggcctgctga ccatcaacgc gctgaccgcc gccgacgggg
      661 tcctcatccc gttggaatgc gagtatttcg cgctgcgggg gatggccctg ctgatggaca
      721 ccatcaccaa gatccgcgag cggcagctca accccgacct ggagatcgtc ggcatcctgg
      781 ccaccatgta cgatgcccgc acggtgcacg gccgcgaggt gctcggccgg gtcgcggacg
      841 ccttcggcga cctggtcttc accaccgtga tcgccaagac gatccgcttt gccgaggcgc
      901 cggtggcggg gcagccgatc ctcacctacg cgagcgactc ggccggcgcc gccgcctacc
      961 gcgacctggc caaggaggtg gcagccgcat gaagaaccgg gccagcctgc ccggcgccga
     1021 cgagctgttc cgggtcacct ccatcgaggc gggccgcggc gccccccggg ccaagccgga
     1081 gtcagcccca acgccatccc cggctccggg gaaaccggcc gcggtcaaag cggtgaagaa
     1141 gcccccagcg ccgcagcagc agccgcccga gcgcgccccg agcgggcggc ggcgccacga
     1201 cgagaagatc accttctact gcaccgccga ggaactgatg gacctggagg acgcccgcct
     1261 gcgcctgcgc cgcgaccacg gcatcgccgt ggaccgcggc cgcctggtcc gcgaggcgct
     1321 cgcccaggtc ctctccgagc tggagcagca gggcgcggac gccccgctgg tgcgacggct
     1381 gtccggcgct cgatgagcgc cgcatgagcc gcatgagccg catgagggcc gtcgacctga
     1441 tcgcgcgcaa gcgcgacggc ggcgagatcg cccgcgcgga cctgcgcgcg ctgatcctcg
     1501 gctacgccag cggggcggtc cccgactacc agatggcggc cttcctgatg gccggctacc
     1561 tgcgcggctt cacccgccgg gaggccgagg cgctcaccga ggcgatggtc gcctccggct
     1621 ccaccctcga cctcggcaag ctccagggac gcaccgttga caagcactcc accggcgggg
     1681 tcgccgacgc caccaccctg atcgtcgggc cgctggccgc cgcgctcggc ctcaagatgc
     1741 tgaagctgtc cggccgcggc ctcggccaca ccggcggcac gctcgacaag ctcgagtcga
     1801 tccccgggtt cacggtcgag cgcagcgcca ccgagctgct cgaccaggtc gaccgcgttg
     1861 gcctcgcggt cgccgcccag agcgccgacc tggtccccgc tgacaaggcc atgtacgcgc
     1921 tgcgcgacgt caccgccacg gtggccagca ccgcgctgat cgcctccagc gtgatgagca
     1981 agaagctcgc cgggggagcg agctgcgtcc tgctggacgt caagaccggc tcgggcgcgt
     2041 tcatgaaaga cccccgggca gccgcgtcgc tggcgggcct ctgcgtcgag atcggccgtg
     2101 ccgccggccg gcggaccgcc gcgctggtca gcgacatgag ccagccgctc ggcgacctga
     2161 tcggcaatgc cgtcgaggtt cgcgaggcgg tcgaggtgct gcgcggcgag cggggtggcc
     2221 gcctgctcga cctctgcctc accttgactg gccagctcgc cgcgctcgcc ggggccgccc
     2281 cgcatcttga cgccgggcgg gagcgggccc gctacgtcct ggagggcggc cacggcctgg
     2341 agaagttccg tgcgttcgtc gccgcgcagg gcggcgaccc ggcggtcgcc gacgaccccg
     2401 ccgcggtcct gccggctgcc ccgctcgccg tcccggtcca cgccgacgcc ggcggcttcc
     2461 tcgccgccgt cgacgccgaa gcggtcggct gggcggccgc caccctcggc gccggccgtc
     2521 agcgcaagga cgaccccatc gacccggcgg tcgcgatcga gctgcccacc aagatcggcg
     2581 accacgtcga agccgggcag ccgatcgcca ccatccgcgc ccgctccgag gccgacgcgg
     2641 aacgggccga ggcggcgctg cgggccgccc tgcggtggag tgagcagccg ccgccggccc
     2701 ccccgctggt ccaccagctg atcgggccgt aagcgccagc tggcgcggcc acgacaccgt
     2761 ggccggcacg ctggtgctcg cctcgtgcta cggtgaccca gaacgctggt caggtttcgg
     2821 cgtcgcaatt cttgtgccga cggtgggccg aggcgcggag cggcgcgagc cgggacacct
     2881 tgaccagcgt tctgagcc
//
LOCUS       DASWDC010000209         7069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_884338, whole genome shotgun sequence.
ACCESSION   DASWDC010000209 DASWDC010000000
VERSION     DASWDC010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884338"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VF995_06745"
     CDS             <1..1004
                     /locus_tag="VF995_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9343295.1"
                     /translation="AARAASALADSAAGTGAGTGAATAPLPTGSGLGREQMLQVKGLW
                     VIDDEHGGARARVAGLDLVVHRGEIHAVAAADGEGAGELIEAVLGLRRPDGGRIYLGD
                     DDVTRASVRARRGHGLGYLPPDEVPGGLVGDLSLRHNALLGRGGRGTRRRAAMLAAAV
                     APGLTHLPAWVVSRGDRQRLLLARELADRPSALLAVCPTRGLRRDEAEPLWQVLRAER
                     DHGLAVLLWSADPEELIALADRVTVVMGGHVVAELAGPTLTTVALSTAMQGRPVPPAD
                     AVEGEAVRRQGSAAEPARARRHQLRPGRQWARPWRWPLGRRLGRRPGRGGAGGSADR"
     gene            1106..2221
                     /locus_tag="VF995_06750"
     CDS             1106..2221
                     /locus_tag="VF995_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343296.1"
                     /translation="MAVAALVAVGAAAAMLALARGWSPVEAARLVASAGLLRPGSLAA
                     AAKAATPLLLAGLAATIALRAGLVNLGIQGQAMAGALAATLAGAHLAAPPGLRLALVL
                     LAGMAGGFAWTLLPTLLAWRGVPELLSTVLASGLALAVLPAGAELPRVRSSAVVGGMP
                     GAPAPGALTWMLPLALLAVLAWWLAARFSRFGLKLRVLGMARHEALLAGVRPSITLAT
                     ALFLSGAVAGLCGLQDALAPGGPHAAAGLAGGAPLDLDLAAGPGLTYGYGLAGVAVAL
                     AGRGSPAGLVAAALAFPLLGQVAAAAHMPAQAAAVLQGVALIAVLAATALARRLAAPA
                     AARRFVSLLEGQAQASGADATSSAATAATEPAGAASA"
     gene            2214..3125
                     /locus_tag="VF995_06755"
     CDS             2214..3125
                     /locus_tag="VF995_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343297.1"
                     /translation="MLEPLRALAPALLVATVLRAAAPLQLAVLGAVISQRAAVLNLAL
                     EGAMALGAAAGAWVAVGHGPLAGLLAGVVAGGAVGALQAVAVIGLRVDQLATGIALDL
                     LALAAATGLAAHSRLAGNLGLAAPRHLPAAALALSVPGLGHGTLRIPAMALVAGALDL
                     LTWLVLRFTPVGLRLRALAEEPEAAERAGVATGPLRAGALLAAGALAGLGGAALPLDG
                     AAAGSGVALAGIAQGRGFVALAIALIAGRSLLAAALLCLPLGAAWPLPAGRGGAILWI
                     ALLLALALRIRRARMPRALNWRADPSA"
     gene            complement(3097..3375)
                     /locus_tag="VF995_06760"
     CDS             complement(3097..3375)
                     /locus_tag="VF995_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX9343298.1"
                     /translation="MRVTEPAQKAVRYAQTEAGRLGQDHVATEHLLLSLLHDEEYLAL
                     AVLGELGLAPERARRGSTRPPARTCAQRTKSGYSSSVPAVRLRGRRAS"
     gene            3689..5131
                     /gene="pyk"
                     /locus_tag="VF995_06765"
     CDS             3689..5131
                     /gene="pyk"
                     /locus_tag="VF995_06765"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX9343299.1"
                     /translation="MAGERTKIVATLGPASGDPVMLGAMLEAGVDVVRLNFSHGARED
                     HLARLELVRRLAAERGRNVAVLADLQGPKIRVGTVGPDGVTLAAGGDCELLAGAERAP
                     AGVVPVVYERLAADVAPGDRILLDDGAIELRVRSVHGEHIACQVRGGGVVRSGKGVNL
                     PGVRVSAPSLTAKDHADLAAAVAAGVDYVALSFVRRAADVLEVRQAIAALGGEMPVVA
                     KLERPEAVEQLDEILAVTDAVMVARGDLGVELAAEQVPPLQKEIIARANAVGVPVITA
                     TQMLESMVTNARPTRAEVSDVANAIFDGTDAVMLSAESAVGAWPLEAVATMGRIAAAA
                     EASPNLNHPPPPAGGVLDVAATVCKAAVQVAADLHARAIVAFTESGATARFVSRFRPR
                     APIVGLTPYEPVRRRMTLYWGVEVPATVAPTSEMLAMVAQADQRLCADGTCRPGDVVV
                     IVAGTPGWRGATNRLIVHRVGEEGDIQRPA"
     gene            5259..5729
                     /locus_tag="VF995_06770"
     CDS             5259..5729
                     /locus_tag="VF995_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343300.1"
                     /translation="MRAEIGEAYEASTSALQAAKGQRLSLRRALGEFERALASPVTDP
                     VEGCIARLITAVQHLRVVFALHVDVTEAPDGLYEEILDNAPRLANKINRFKREHASIS
                     DAIERGVADLGDASALTPELRGQLVKLFDDLTRHRKRGLDLVYEAYHVDIGGES"
     gene            complement(5921..6871)
                     /gene="arcC"
                     /locus_tag="VF995_06775"
     CDS             complement(5921..6871)
                     /gene="arcC"
                     /locus_tag="VF995_06775"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX9343301.1"
                     /translation="MGEAPVVVALGGNAIAPFGARETLRTQYQRTQRTAEHLVHLSEG
                     ERPLLLTHGNGPQVGESLLRSEAGAATGQLPRLPLDALVADTQGRMGFMLQQCLDNAL
                     QAAGMRRSVAALLTRTVVNPADPAFGAPSKPIGDFYDEAAARRHMAADGWVMREDAGR
                     GWRRVVPSPQPHEVLEEGAIRALLTLGVIVVAAGGGGIPVVRQPDGSLTGVEAVVDKD
                     LTAALLATQIGADTLLILTGVDHVALDFGRPTQRDLHEVSAAELAAYAAAGQFPPGSM
                     LPKIQAALTFLAQGGQRAIITTPELSEKALTTTTGTQVTR"
BASE COUNT          786 a         2560 c         2749 g          974 t
ORIGIN      
        1 tcgccgcgcg ggcggcctcc gcgctggctg attccgccgc cgggaccggg gctgggaccg
       61 gggccgccac agctccgctc ccgacagggt ctggcctggg gcgggagcaa atgctgcagg
      121 tcaaggggtt atgggtgatc gacgacgaac acgggggagc gcgggcgcgg gtcgccgggc
      181 tggacctggt ggtgcaccgt ggcgagatcc atgccgtcgc cgccgccgac ggcgagggcg
      241 cgggcgagct gatcgaggcg gtgctcggtc tgcggcggcc cgacggtggc cgcatctacc
      301 tcggcgatga cgacgtcacc cgggcgagcg tccgcgcccg gcgcgggcac gggctcggct
      361 acctcccgcc ggacgaggtc cctggcgggc ttgtcggcga cctcagcctg cgccacaacg
      421 ccctgctcgg ccgcggcggg cgtgggacgc ggcggcgggc cgccatgctc gcggcagcgg
      481 tcgctccggg gctcacccac ctgccggcct gggtggtctc gcggggcgac cggcagcggc
      541 tgctgctcgc ccgtgaactc gccgaccggc ccagcgcgct gctggccgtc tgccccaccc
      601 gggggcttcg gcgcgacgag gcggagccgc tgtggcaggt gctgcgggcc gaacgcgacc
      661 acgggctggc ggtgctgctg tggtcggccg accccgagga gctgatcgcc ctggccgacc
      721 gtgtcactgt cgtgatgggt ggacatgtgg tcgcggagct ggctgggccg acgctcacca
      781 ccgtcgcgct cagcaccgcg atgcagggac ggccggtccc gcccgccgac gccgtggagg
      841 gcgaggccgt gcggcggcag gggagtgccg ccgagccggc gcgggcgcgg cggcaccagc
      901 tcaggcccgg gcggcagtgg gcccggccgt ggcgctggcc gctcgggcgg cggctcgggc
      961 gccgccctgg gcgaggcggc gccggtggga gtgcggaccg gtgagggcgg acgccgccgg
     1021 tgccggccgg cctcccgtcg ggacccgccc ccggagccgg agatggacgc cgtgcctgct
     1081 ggtccccgcc gccacggtgg catccatggc ggtcgccgcg ctggtcgcgg taggcgccgc
     1141 cgcagcgatg ctcgccctgg ccagggggtg gagcccagtg gaggcggcgc ggctcgtcgc
     1201 cagcgccggg ttgctccgcc cggggtcgct cgcggccgcc gccaaggcgg ccacgccgtt
     1261 gctgctggcc gggttggcag ccacgatcgc cttgcgcgcc ggcctggtca acctgggaat
     1321 ccagggccag gccatggccg gggcgctggc ggccacgctg gccggggcgc acctcgcggc
     1381 cccgcccggt ctgcgcctgg cgctggtgct gctcgccggg atggccggcg ggttcgcatg
     1441 gacgctgctc cccaccctgc tggcctggcg gggtgtgccg gagcttctct cgaccgtact
     1501 ggcgagtggg cttgccctcg ccgtgcttcc cgccggggcc gagctgccgc gggtacggtc
     1561 gagcgccgtg gtcggcggga tgcccggagc cccagcaccg ggcgcgctca cctggatgct
     1621 gccgttggcc ctcctcgccg tgctcgcctg gtggctcgcc gctcgcttca gccgcttcgg
     1681 cctgaagctg cgagtcctgg gcatggcccg gcacgaggcc cttctcgccg gcgtccgacc
     1741 gagcatcacg ctggccacgg cgctcttcct ctccggcgcc gtcgcgggcc tgtgcggcct
     1801 ccaggacgcc ctcgccccgg ggggcccgca cgccgccgcc gggctggctg gcggcgctcc
     1861 gctcgacctg gacctggccg ccggtcccgg gctgacctac ggctacggcc tcgccggtgt
     1921 cgccgtcgcc ctggccggac ggggcagccc cgccggcctg gtggccgccg cgctggcgtt
     1981 cccgctgctc ggccaggtcg ccgcggccgc ccatatgcca gcgcaggccg ccgccgtcct
     2041 gcagggcgtg gcgctgatcg cggtcctggc cgcgaccgcg ctcgcccgcc gcctggccgc
     2101 acccgccgcc gcgcgccgct tcgtgtcgct gctggagggc caggcgcaag cgtccggcgc
     2161 cgacgcgacc agcagcgcgg ccacggccgc caccgagccg gcaggtgccg ccagtgcttg
     2221 agccgttgcg ggcgctcgcg cccgcgctgc tggtcgcgac cgtcctgcgg gcggcggcgc
     2281 cgctgcagct ggcggtgctc ggcgccgtga tcagccagcg ggcggcggtg ctcaacctcg
     2341 ccctcgaagg ggcgatggcg ctcggcgccg ccgcgggcgc ctgggtggcg gtcgggcacg
     2401 gaccgctcgc cggcctgctg gccggcgtcg tggccggggg cgctgtgggc gcgttgcagg
     2461 cagtggccgt gatcggcttg cgagtcgacc agctcgccac tggaatcgcg ctcgacctgc
     2521 tcgcgcttgc cgccgccacc gggctcgccg cgcattcccg gctcgccggc aacctgggtc
     2581 tggccgcgcc acggcatctg cccgcggccg cgctggcgct cagtgtgccc ggccttggcc
     2641 acggcaccct ccgcatcccg gcgatggcgc tggtcgccgg ggcgctcgac ctcctcacct
     2701 ggctcgtgct gcgcttcacc ccggtcgggc tgcggctgcg cgcgctcgcc gaggagcccg
     2761 aggcagccga acgcgccggg gtggcgaccg gccccctgcg cgccggggcc ctgctcgcgg
     2821 ccggggcgct cgccggcctc ggcggcgccg ccctcccgct cgacggtgcc gccgccggct
     2881 ccggcgtcgc cctcgccggg atcgcccagg ggcgtgggtt cgtcgccctc gccatcgcgc
     2941 tgatcgcggg ccgctccctg ctggccgccg cgctgctgtg cctgccgctc ggcgctgcct
     3001 ggccgctccc agccggccgc ggcggtgcca tcctgtggat cgccctgctg ctcgccctcg
     3061 ccctgcggat ccgccgggcg cgaatgcccc gcgccctcaa ctggcgcgcc gacccctcag
     3121 cctgacggca ggaacgctgc tcgagtatcc cgacttcgtc cgctgggcgc aggtcctggc
     3181 cggtggtctc gtcgacccgc ggcgcgccct ctccggcgcg agccccaact cgccgaggac
     3241 cgcgagcgcc aggtattcct cgtcgtggag gaggctgagc agcaggtgct cggtggcgac
     3301 gtggtcctgg ccgagccggc cggcctccgt ctgggcgtag cggaccgcct tctgggcagg
     3361 ttcggtgacg cgcatcggcc aacctcctcg cgccggcttc ggaccgcgac cattcccgcc
     3421 agcatgccaa gcgccaggtc gtcgggttgc cgcggagggc ctgggcgagc agttcgagga
     3481 gcgtgtgcca gcctgcaggt cgcggctggc gtgctcggag gcgaggtggg gcggttcggt
     3541 gtgggtgagg atcagcacct tccttccggt ggggtggcac gcctcgggcg gctcggcacg
     3601 ctcgcgttcc gtatagcctt tccctcagcg agcgggacgg ccaagtcggc agcgacaaca
     3661 agcggacaag acagaggagc gcggaggttt ggcgggggag cggaccaaga tcgtagccac
     3721 gctggggccg gcatcggggg atccggtgat gctcggggcg atgctcgagg ccggggtgga
     3781 cgtggtgcgg ctcaacttct cgcatggggc gcgggaggat catctggcgc ggctggagct
     3841 ggtgcggcgg ctggccgctg agcgggggcg caacgtggcg gtgctggccg acctgcaggg
     3901 gccgaagatc cgggtgggca cggtcgggcc ggacggggtg acactcgccg ctggcggcga
     3961 ctgcgagctg ctggccggcg ccgagcgggc cccggccggg gtggtgccgg tggtctacga
     4021 gcggctcgcc gccgacgtcg cgcccgggga tcgcatcctg ctcgacgacg gcgcgatcga
     4081 gctgcgggtc cgctcggtgc atggcgagca catcgcctgc caggtgcgcg gcggcggggt
     4141 ggtgcggtcg ggcaagggcg tgaacctgcc gggggtgcgg gtgtcggcgc cctccctgac
     4201 cgccaaggac cacgccgacc tggccgccgc ggtcgcggcc ggggtcgact acgtggcgct
     4261 gtcgttcgtg cgccgcgccg ccgacgtgct ggaggtcaga caggcgatcg ccgcgctcgg
     4321 cggcgagatg ccggtggtcg ccaagctgga gcggccagag gcggtcgagc agctcgacga
     4381 gatcctggcg gtcaccgacg cggtcatggt cgcccggggc gacctcggcg tggagctggc
     4441 ggccgagcag gtcccgccgt tgcagaagga gatcatcgcc cgcgccaacg ccgtcggcgt
     4501 gccggtgatc accgccaccc agatgctcga gtcgatggtg accaacgcgc ggccgacccg
     4561 agcggaggtc agcgacgtgg ccaacgccat cttcgacggc accgacgcgg tcatgctgtc
     4621 ggcggagagc gccgtcggcg cctggccgct ggaggcggtc gcgaccatgg gccgcatcgc
     4681 cgccgccgcc gaggcgagcc cgaacctcaa ccacccgccc ccgccggccg gcggcgtgct
     4741 cgacgtggcg gcgaccgtct gcaaggcggc ggtgcaggtc gccgccgacc tccacgcccg
     4801 cgcgatcgtg gcgttcaccg agagcggcgc gaccgcccgg ttcgtgtcgc ggttccgccc
     4861 tcgcgccccc atcgtcgggc tgacccccta cgagccggtg cggcgccgca tgaccctcta
     4921 ctggggcgtc gaggtccccg ccacggtcgc gccgaccagc gagatgctcg ccatggtcgc
     4981 tcaagccgac cagcgtctct gcgcggacgg cacctgccgg cccggcgacg ttgtggtaat
     5041 cgtggccggg acgcccggct ggcgtggcgc gaccaaccgt ttgatcgtcc accgggtggg
     5101 ggaagaagga gacatccagc gccccgccta atttcagctt gctttggccg tttcgtcccg
     5161 ccggggcact tctagtcacg atgaactaga tgacaacggg agggttttcg gtaacactgc
     5221 cgagaccgga gcggccgggt cgacgaaggg ggatgaagat gcgagctgag ataggtgagg
     5281 cttacgaagc gtcgaccagc gcgctccagg ccgcgaaagg acagcggctc agtctccggc
     5341 gggccctcgg cgagttcgaa cgggcgctgg caagccccgt caccgacccg gtcgagggat
     5401 gcatcgcccg cctgatcacg gccgtgcagc acctacgggt ggtcttcgcc ctgcacgtgg
     5461 acgtgaccga ggcgccggac ggcctctacg aggagatcct cgacaacgcc ccccgcctcg
     5521 ccaacaagat caaccgtttc aagcgcgagc acgccagcat cagcgacgcg atcgagcgtg
     5581 gcgtggccga cctcggcgac gccagcgcgc tcaccccgga gctgcgcggc cagctggtga
     5641 agctgttcga cgacctcacc cgccaccgca agcgcggcct cgacttggtc tacgaggcct
     5701 accacgtcga catcggcgga gaaagctagg gataccccgg gtgcgagact gcgccttggc
     5761 gtgggtggcc cggcggggtc ccgttctccg ctccgagaag caggtcgcga gaacgggacc
     5821 ccgccgggcc gacggtggtc catacggctc cggctcgcac tcacccctat ggaggtgtcg
     5881 agtgggggcg cggcgcttgt ggcttgggga tggtatgtcg tcagcgggtt acctgggtgc
     5941 cggtggtggt ggtgagggcc ttctcggaga gttcgggggt ggtgatgatt gcgcgctggc
     6001 cgccctgggc gaggaaggtg agggcggctt ggatttttgg gagcatgctg ccggggggga
     6061 actggccggc ggcggcgtag gcggcaagtt cggcggcgct gacctcgtgg aggtcgcgct
     6121 gggtcgggcg gccgaagtcg agggcgacgt ggtcgacgcc ggtgaggatg agtagggtgt
     6181 ccgcgccgat ctgggtggcg agcagggcgg cggtcaggtc cttgtcgacc actgcctcca
     6241 cgccggtcag cgagccgtcg ggctggcgta ccaccgggat gccgccgcct ccggcggcga
     6301 ccacgatcac gccgagggtg agcagggcgc ggatcgcccc ttcctcgagc acctcgtggg
     6361 gttgcggtga gggcaccacc cggcgccagc cgcgaccggc gtcctcccgc atgacccagc
     6421 cgtcggcggc catgtggcgc ctcgcggccg cctcgtcgta gaagtcgccg atcggcttgg
     6481 agggggcacc gaacgccggg tcggcggggt tgaccaccgt gcgggtcagc agcgccgcca
     6541 ccgaccgccg catcccggct gcctgcaggg cgttgtcgag gcactgctgc agcatgaacc
     6601 ccatgcggcc ctgggtgtcg gcgacgaggg cgtccagggg gagccgcggg agctgcccgg
     6661 tcgccgcccc cgcctcgctg cgcagcaggc tctcgcccac ctgcgggccg ttgccgtggg
     6721 tcagcagcaa cggccgctcg ccctcgctga gatgaaccag atgctccgcg gtccgctgcg
     6781 tgcgctgata ctgggtcctc agcgtttccc gggcaccgaa gggggcgatc gcgttgcccc
     6841 ccagcgccac cacgaccggc gcttccccca cgtccaggcc ccccttgcga ccgccggcac
     6901 gccgttgtgc cggaaggccg caaggctacc ggatcggtag gccccagagg gcgaaccagc
     6961 gagtcaggtc gggctcgacc ggcaggtccg agccgagggt cgcgcggacg cggagctcca
     7021 gctcgttgtc gcgcttctcg ccgtcgcgcg gcgcgaacgg gtagaaggt
//
LOCUS       DASWDC010000210        12184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1386534, whole genome shotgun sequence.
ACCESSION   DASWDC010000210 DASWDC010000000
VERSION     DASWDC010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12184
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386534"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VF995_06780"
     CDS             <1..771
                     /locus_tag="VF995_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX9343302.1"
                     /translation="QPEVEPLVAGTATLTGAPGCVRVVGVKGAPAPLLTGWSAGLGVE
                     IRDGAVADGHDAAGQLGPFTVLTAAELALAVAAAFGDDTIEWAAVGGRSLLLQSGACG
                     RRPAPAPQDRNQVRVEAAGAHLTTAAGPARVASSAELHAHADAMAHGERFQGQPAAPG
                     IAAGPARVAGGSPQRLPLPDRAVVVARYPLPHLAPLLWGAAAVVTFGGSPAAHLFQVA
                     RSLGVPAVAGCGGLDGRLAPDADAVLAVDGGSGEVGLA"
     gene            843..1709
                     /locus_tag="VF995_06785"
     CDS             843..1709
                     /locus_tag="VF995_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9343303.1"
                     /translation="MTGRAFVLQVLNGISFGALLFLLSSGFQLIFGLMRIVNLAHGSF
                     YLVGGYIGLSVLRAGGNFWLAVGAAALSVATIGLVVELGLLRRLRGQAMPEVLLTVGL
                     SLIFADLSLARWGGDPQILPSPPGLQGSIHLGSLTYPTFRLFVLALGALVAAGLWLLL
                     SRTRIGAIVRAGADDQEMVMALGVNIRAVFAGAFTIGALLAGIAGIVGGVFLGLYPGA
                     DGEILLFALVVVILGGLGSLQGAMVGSLLVGLIDAFGRELVPSLSYFLLFAPMALVLI
                     FRPRGLFGKATT"
     gene            1706..2785
                     /locus_tag="VF995_06790"
     CDS             1706..2785
                     /locus_tag="VF995_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9343304.1"
                     /translation="MTAGGRLPGPRWRLTGALKLGGLALVLALLVSLPFLTGSFYVSL
                     ATQGLYLGLFALSINLLAGYGGMITLGQAGVSGIAGYGLAIFLTRFNWPLGVAIVAAL
                     ALTVAAAAFLGALAMRTSGVYFLMVTLAEGMVIWGIAQRWNTVTGGDNGIRGVTRPGF
                     ASVYWKYYYFTLAVVALCVALIALVVRSPFGLTLRGIRESEGRMGPLGYNVALHKLVA
                     FVVAGVFAGVSGLLLAMDNNFFSPSSVHLKASADGLLMSILGGMGTLTGAFVGSGVFV
                     FITNYVSGYLTRWPTLLGLIFVLVILFARDGIVGAWNRWVWAPLLRRAGQAEAAELAE
                     EAAREVASGLLSGPPAGVGTKGGPQ"
     gene            2782..4098
                     /locus_tag="VF995_06795"
     CDS             2782..4098
                     /locus_tag="VF995_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9343305.1"
                     /translation="MRHRRSSSVLLLAVLALVLAACHSPGSRSSGGKSGTIKIGFISP
                     VTGTTAASGIEMLNGWKLYWKLNPNTVAGRKITWTSEDDAGDLNTGLQKARKLVEQNG
                     DQVLVGPLLANVSYAVAQYLSTQPNVVGLNPVASADDLSQRKAVKNYVRTGGWQSSTP
                     PQVAGDWAWQQGYRRAITLCQDYAFGQESCGGFINTFTDHGGKIVKQLWAPLGTTDYS
                     SYLAQINPKSADVVYIATVGADSVRVIKGWSQFGLKSRIPLIGQETTLEQSLIRAMGD
                     AAVGLQSFGHYAEGRDDPMTQQFDTAYEQEYHLIPSYYACAAYTGAQWFAQALQQTGG
                     DMSDVAHFTDVLRGIKLTDSCMGPMHLDSNGGNVFNVYLRQVAKDSQGRNYNKVLKSF
                     PDISQFYWYKPEAFLAQPVYSRQYLGENWPTSCDAYVTGKCPSQSQ"
     gene            4117..4875
                     /locus_tag="VF995_06800"
     CDS             4117..4875
                     /locus_tag="VF995_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9343306.1"
                     /translation="MTQAGAALRLEGVTRDFGGLRAVDEVTLTIPPGERRAIIGPNGA
                     GKTTLFKLISGEDRLTGGRVELFGRDITNLPCHRVTRMGLGRTYQITQVFPALSVEEN
                     VLLAAQGARHGKFAMLRPVRGRRELVGTAHEAISRAGLDSVAKARAAELGHGQQRQLE
                     LALALAAAPRILLLDEPGAGLAAAERARMRELVRELPRQTTILLIEHDMELALGLADR
                     VTCMHDGRVVAEGSPGQIKNDQRVQDIYLGRASR"
     gene            4875..5612
                     /locus_tag="VF995_06805"
     CDS             4875..5612
                     /locus_tag="VF995_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9343307.1"
                     /translation="MLKVSGLACAHGLAAVLSDVSIEVPDATVVALLGRNGMGKTSLC
                     RCVMGLEPPRVTAGQIDLDGTKLSGLPPYARAKLGIGYVPQGRRVFPSLDVVENLTMA
                     ARPAPSGAGGGGGDGIEAWTLDRVWAMFPRLADRKHHPAGQLSGGEQQMLAIARALMT
                     NPHLLVMDEPSEGLAPVIVESLGEHLQALRASSLSILLVEQNYTLAMTLADTVYVLEN
                     GRIVFHGPPATLDAADDVKRRHLGVGV"
     gene            complement(5977..6546)
                     /locus_tag="VF995_06810"
     CDS             complement(5977..6546)
                     /locus_tag="VF995_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9343308.1"
                     /translation="MIVVDTNVVSELMRPSPTAAVVAWVRGNERELYTTSITLAEIAY
                     GIERLPGGPRKRLLRSAAEEVFAAFEERILSFDAKAALAYATVVSGRDRAGRPIDGFD
                     AQIAAICRVLQAALATRNLKDFRDTGIDVIDPWRPDPHCSVSGKRQEPYRLVPHESSL
                     RPGLGDSGLKKPAGELENEPTIDEAPPSS"
     gene            complement(6543..6791)
                     /locus_tag="VF995_06815"
     CDS             complement(6543..6791)
                     /locus_tag="VF995_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HEX9343309.1"
                     /translation="MMAAVSIRNLDEETKRLLRVRAAQHGRSMESEIRAILQEAVREP
                     ERSEGLFTTLLDRFGALGGVELDLPARSVPARAADLPE"
     gene            complement(6999..7397)
                     /locus_tag="VF995_06820"
     CDS             complement(6999..7397)
                     /locus_tag="VF995_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9343310.1"
                     /translation="MGLALLDTSIVIAALNRHDALHRAAARVLLAERDRHALGISALT
                     YAELLVGPLRAGGDAVQIVERFAAQSRILDVSASIARLAAELRAKRGLKLPDAVIVAT
                     GLRHEADLILTGDARWQGIETVRVVASGDG"
     gene            complement(7388..7645)
                     /locus_tag="VF995_06825"
     CDS             complement(7388..7645)
                     /locus_tag="VF995_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343311.1"
                     /translation="MSEKEKKPPRRRGFTRLSRKHQVTVPVAVLAEAGIQAGESLRVQ
                     ALGPGRVILTRADDRLEDLLGALGSDVYPEGYLQELRAEWD"
     gene            complement(7707..8084)
                     /locus_tag="VF995_06830"
     CDS             complement(7707..8084)
                     /locus_tag="VF995_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9343312.1"
                     /translation="MGKEFHTAHRWDLAWQDDSEVLALGHGVQVKVYHHDPERGHTDM
                     LIKFPPGYVEPEHAHASEHSVVVLEGLQIANGDPLHPGDSCYGPPEVPHGPFEYPEGC
                     VVFASFRGGSALHRYPGSPAGDI"
     gene            complement(8096..9130)
                     /locus_tag="VF995_06835"
     CDS             complement(8096..9130)
                     /locus_tag="VF995_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9343313.1"
                     /translation="MSVGAGERGGRMRAVLLTDINAVSVEEVPEPDPGGRALLRVRRA
                     GLCGTDLKIASGQIPVRVPLIIGHEMVGTVLAAGARGLVPEGSRVLVDPGIACGHCEL
                     CLRDRGYLCRQGGLMGRDTDGGFAELVAVDELQLHPLPQTIGDDAAALLQVLATCVHA
                     ETMLEVFPGQSAVVVGLGVAGQLHLQLLRARGITTIVGVTRSAAKRELAAKLGASATA
                     SPGEAASVVAEATGGRGADLVVEAAGTADTLAQSIELAGSGATILVFGTTTIAERLPT
                     YQLYYKELTLLNPRAARPRDYDRAIQLCAAGALRLEPLLTATFPLEEAPSAFAACREP
                     SQLKVALDVS"
     gene            complement(9208..9642)
                     /locus_tag="VF995_06840"
     CDS             complement(9208..9642)
                     /locus_tag="VF995_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX9343314.1"
                     /translation="MTTLTLQQAAIIIDAGLARGRERGFEPLTIAVLDPGGHPLCLKR
                     EDGSGILRPQIAVGKAWGALGMGYGSREVGRRAADAPVFFSALFEVSGGRMLPAPGGV
                     LVRSPEGELLGAVGVTGDNSDNDELCALAGIEAAGLVAQPAA"
     gene            complement(9659..10351)
                     /locus_tag="VF995_06845"
     CDS             complement(9659..10351)
                     /locus_tag="VF995_06845"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9343315.1"
                     /translation="MPRLIDLSQEIYTGMYVYPGHLKTVVFDHASHEETSERFEGGFS
                     FQTTGLLVNDNGPTHVDSFSHLDPDPEAASIDQMPLELFYGDAICLDVSACRPRTDVE
                     PAQLEAALEISGLELRRGDILLLYTATWNRYHGTREYLTSFPGLGAAGSQWLVDRGVK
                     TFGVDSPTPDNPTSRSYPCHMMCRAEHITHYENLANLDKVSGRRFTFAGFPLRIRGGH
                     GGPTRAVALLDD"
     gene            10488..>12184
                     /locus_tag="VF995_06850"
     CDS             10488..>12184
                     /locus_tag="VF995_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX9343316.1"
                     /translation="MTDQPDQMLIEWDVPIEMDDGVVLRADVFRPPPQEEGQRRDPVL
                     LSYGPYAKGLAFQDGYPEQWAAMCREHPDVPAGSSNRYQNWETIDPEKWVPAGYACVR
                     VDARGAGRSPGYLDPLSPRETRDLYHCVEWAGTREWSNGKVGLAGISYYAINQWHVAA
                     LQPPHLAAICPWEGAADWYRDMTHHGGIACTFWANWYRQQVLGVQHGVGDKGPSSRVT
                     GEPVAGPETLDTEELERRRTDLEAEIIAHPLDDAYHRERSPDWSRVTAPLLSAANWGG
                     QGLHLRGNIEGFTQAASRRKWLEVHGLEHWTHFSTDYGRGLQRRFFDHFLKGEANGWN
                     FQPPVLLQVRHADGSFTQRAERAWPLERTRWARLYLDPSAGRAGERGDAVHGTPPAER
                     AGKRSTVVHGRLAEAEAEAGHSLAARLPLTSARATFAALGDGITFTTPPLAAETEITG
                     PVAAKLFVSSSTSDADLFVVLRVLDPEGREVTFQGAIDPHTPVAQGWLRASRRKLDPA
                     LSLPHRPWHTHDEHQPLVPDEVYELDIEIWPTCVVVPAGYRLALTVRGRDYEYEDAGG
                     "
BASE COUNT         1691 a         4293 c         4357 g         1843 t
ORIGIN      
        1 cagccggagg ttgagccgct cgtcgccggc accgccacgc tgaccggggc gccaggctgc
       61 gtgcgggtgg ttggcgtcaa gggcgcgccc gcgccgctgc tgaccggctg gagcgcgggc
      121 ctgggggtgg agatccgcga cggcgcggtc gccgacggcc acgacgccgc cggccagctc
      181 ggcccgttca cggtgctgac cgccgccgag ctggccctgg cggtcgccgc cgcgttcggc
      241 gacgacacca tcgagtgggc ggccgtcggc gggcggtcgc tgctgctcca gtcgggcgcg
      301 tgcgggcggc gcccggcgcc cgcaccgcag gaccggaacc aggtccgggt ggaggcggcg
      361 ggggcgcacc tgaccacggc ggccggtccg gcccgggtgg cgagcagcgc cgagctgcac
      421 gcccacgcgg acgcgatggc gcacggcgag cgcttccagg gccagcccgc cgccccgggg
      481 atcgccgccg gaccggcccg ggtcgccggc gggtcgccgc agcggctgcc tctcccggac
      541 cgggcggtgg tcgtggcccg gtatccgctc ccccacctgg cgccgctgct gtggggcgcg
      601 gcggcggtgg tgaccttcgg cgggagtccg gccgcgcacc tgttccaggt cgcccgctcg
      661 ctcggcgtcc cggcggtggc gggctgcggc gggctggatg ggcggctcgc gccggatgcg
      721 gacgccgtgc tcgcggtcga cggcggctcc ggcgaagtgg ggcttgccta atgcatttta
      781 tatgctgcag tatcgcggtc ggagcccccg ctggcgctgg agcccctcgg aggacgatgc
      841 cggtgactgg aagagcgttc gtcctgcagg tcctgaacgg gatttccttc ggcgcgctcc
      901 tgttcctgct gtcgagcgga ttccagctga tcttcgggct gatgcgcatc gtcaacctgg
      961 cgcacgggtc gttctacctg gtcggcggct acatcggcct gtcggtgctg cgcgccggcg
     1021 ggaacttctg gctggcggtc ggcgccgccg ccctgtcggt cgcgacgatc gggctggtgg
     1081 tcgagctcgg cctgctgcgc cgcctgcgtg gccaggcgat gccggaggtg ctgctcaccg
     1141 tcgggctgtc gctgatcttc gcggacctgt cgctggcgcg ctggggcggt gacccgcaga
     1201 tcttgccaag cccgccgggg ctgcagggct cgatccacct cggcagcctc acctacccga
     1261 ccttccggct gttcgtgctg gcgctgggcg cgctggtcgc cgccgggctg tggctgctgc
     1321 tgtcacgcac ccggatcggc gcgatcgtgc gggcgggcgc cgacgaccag gagatggtca
     1381 tggcgcttgg cgtcaacatc cgggcggtgt tcgccggggc gttcaccatc ggcgcgctgc
     1441 tggccgggat cgccgggatc gtcggcgggg tgttcctcgg gctgtatcca ggcgccgacg
     1501 gggagatcct gctgttcgcg ctggtggtgg tgatcctcgg cgggctcggc agcctccagg
     1561 gcgcgatggt cgggagcctt ttggtggggc tgatcgacgc cttcgggcgc gagctggtcc
     1621 cctcgctgtc gtacttcctg ctgttcgcgc cgatggcgct ggtactgatc ttccggccgc
     1681 gcggcctgtt cgggaaggcg accacgtgac tgccggaggg cgccttcccg gcccgaggtg
     1741 gcggctgacc ggcgccctca agctgggcgg cctcgcgctg gtgctggcgc tgctggtgag
     1801 cctgccgttc ctgaccgggt cgttctatgt cagcctcgcc acccagggcc tctatctggg
     1861 gctgttcgcg ctgtcgatca acctgctggc cggctacggc gggatgatca ccctcggcca
     1921 ggcgggcgtc agcgggatcg ccgggtacgg gctggcgatc ttcctgacgc gcttcaactg
     1981 gccgctcggg gtggcgatcg tcgccgcgct ggcgcttacg gtggccgccg ccgccttcct
     2041 cggcgcgctg gcgatgcgca ccagcggcgt ctacttcctg atggtcaccc tcgccgaggg
     2101 gatggtcatc tggggcatcg cgcaacggtg gaacaccgtc accggcggtg acaacggcat
     2161 ccggggcgtc acccggcccg gcttcgccag cgtctactgg aagtactact acttcaccct
     2221 cgccgtcgtg gcactgtgcg tggcgctgat cgcgctggtc gtgcgctcgc cgttcgggct
     2281 gaccctgcgg gggatccgcg agtccgaggg acgcatgggc ccgctcggct acaacgtggc
     2341 gctgcacaaa ctggtcgcgt tcgtggtcgc gggggtgttc gccggggtct ccgggctgct
     2401 cctggcgatg gacaacaact tcttcagccc gtcgtcggtc cacctgaagg cctccgccga
     2461 cgggctgctg atgtccatcc tcggcgggat gggcacgctg accggcgcgt tcgtcggctc
     2521 gggcgtgttc gtgttcatca ccaactacgt cagcggctac ctgacgcggt ggcccacgct
     2581 gctcggcctg atcttcgtcc tggtgatcct gttcgcccgg gacgggatcg tcggcgcgtg
     2641 gaaccgctgg gtgtgggcgc cgctgctgcg ccgcgccggc caggccgaag cggccgagct
     2701 ggccgaggag gcggcgcggg aagtcgccag cgggctgctg tccggccctc ccgccggcgt
     2761 gggaaccaaa ggaggacccc aatgaggcac cgtcgttcca gttccgtgct tctgctcgcc
     2821 gtgctcgcgc tcgtcctcgc cgcctgccac tcgccggggt cgcggtcgtc gggtgggaag
     2881 tcggggacca tcaagatcgg cttcatcagc ccggtcaccg gcaccaccgc cgcctccggc
     2941 atcgagatgc tcaacggctg gaagctgtac tggaagctca acccgaacac ggtcgccggc
     3001 cgcaagatca cctggaccag cgaggacgac gccggcgacc tgaacaccgg cctgcagaag
     3061 gcgcgcaagc tggtggagca gaacggggac caggtgctgg tcgggccgct actggccaac
     3121 gtcagctacg cggtcgcgca gtacctctcc acccagccga acgtggtggg gctgaacccg
     3181 gtcgcctccg ctgacgacct gagccagcgc aaggcggtca agaactacgt ccgcaccggc
     3241 ggctggcaga gctcgacgcc gccgcaggtg gccggtgact gggcctggca gcagggctac
     3301 cggcgggcga tcacgctctg ccaggactac gccttcgggc aggagtcgtg cggcggcttc
     3361 atcaacacct tcaccgacca cggcggcaag atcgtgaagc agctctgggc gccgctgggg
     3421 accaccgact acagctccta tctggcgcag atcaacccca agtccgccga cgtggtctac
     3481 atcgccaccg tgggcgccga ctcggtgcgg gtcatcaagg ggtggagcca gttcggcctc
     3541 aagagcagga tcccgctgat cggccaggag accactctgg agcagtcgct gatccgcgcg
     3601 atgggcgacg cggcggtcgg cctgcagtcc ttcgggcact acgccgaggg ccgcgacgac
     3661 cccatgaccc agcagttcga caccgcctac gagcaggaat accacctcat cccgtcgtat
     3721 tacgcctgtg ccgcctacac cggcgcgcag tggttcgccc aggccctcca gcagaccggc
     3781 ggcgacatga gcgacgtcgc gcacttcacc gacgtgctgc gcggcatcaa gctgaccgac
     3841 tcctgcatgg ggccgatgca cttggacagc aacggcggca acgtcttcaa cgtgtacctg
     3901 cgccaggtgg ccaaggacag ccagggccgc aactacaaca aggtgctgaa gagcttcccg
     3961 gacatcagcc agttctactg gtacaagccg gaggcgttcc tggcgcagcc ggtctactcc
     4021 cgccagtacc tgggcgagaa ctggccgacc tcctgcgacg cctacgtcac cggcaagtgc
     4081 ccctcccagt cgcagtgacc aggtgagggg gcttcggtga cccaggctgg cgcggcgctc
     4141 cgactggaag gcgtcacccg cgatttcggc ggcctgcggg ccgtcgacga ggtgacgctg
     4201 accattcccc ccggggagcg ccgcgccatc atcggcccca acggcgccgg caagaccacc
     4261 ctgttcaagc tgatcagcgg cgaggaccgc ctcaccgggg ggcgggtcga gctgttcggc
     4321 cgcgacatca ccaacctgcc ctgccaccgg gtcacccgca tgggcctcgg ccgcacctac
     4381 cagatcaccc aggtcttccc ggccctgtcg gtcgaggaga acgtgctcct ggccgcgcag
     4441 ggcgcccgcc acggcaagtt cgccatgctg cgcccggtgc gcggccgccg cgagctggtc
     4501 gggaccgccc acgaggcgat cagccgcgca ggcctcgaca gcgtcgccaa ggcgcgggcg
     4561 gccgagctgg gccacggcca gcagcggcag ctggagctgg cgctggcgct cgccgccgcc
     4621 ccgcggatcc tgctgctgga cgagccgggc gcgggccttg cggcggcgga gcgggcccgc
     4681 atgcgcgagc tggtccggga gcttccccgc cagaccacca tcctgctgat cgagcacgac
     4741 atggagctgg ccctgggcct cgccgaccgg gtcacctgca tgcacgacgg ccgtgtcgtg
     4801 gccgagggca gtcccggcca gatcaagaac gaccagcggg tgcaggacat ctacctgggg
     4861 agggcgtcgc gctgatgctg aaggtctccg gcctcgcctg cgcccacggc ctcgccgcgg
     4921 tgctgtccga cgtgtcgatc gaggtacccg acgccaccgt ggtcgccctc ctcggccgca
     4981 acggcatggg caagacgagc ctctgccgct gcgtcatggg cctggagccc ccccgcgtca
     5041 ccgccggcca gatcgacctc gacggcacga agctgtccgg cctccccccg tatgcccgcg
     5101 ccaagctcgg cattggctac gttccccagg gccgccgcgt cttcccctcc ctcgacgtgg
     5161 tcgagaacct caccatggcc gcccgccccg ccccgagcgg cgccggcggc ggtggtggcg
     5221 acggcatcga agcctggacc ctcgaccgcg tgtgggcgat gttcccccgc ctcgccgacc
     5281 gcaagcacca ccccgccggc caactctccg gcggcgagca gcagatgctc gccatcgccc
     5341 gcgccctcat gaccaacccc cacctcctgg tcatggacga gccctccgaa ggcctcgccc
     5401 ccgtgatcgt cgaatccctc ggcgagcatc tgcaggccct gcgcgcctcc agcctgtcga
     5461 tcctcctggt cgagcagaac tacacgttgg cgatgaccct cgccgacacg gtctatgtgc
     5521 tggagaacgg ccgcattgtc ttccacggcc cccccgccac gctcgacgcc gccgatgacg
     5581 tcaagcgccg tcacctgggc gtgggcgtgt aaagggtatt cgggcagaga ctgagctgat
     5641 cgggcgattg gcgtagttga cgagctaggt ctggcatgcg atggttacgg tggcagacca
     5701 tacccgcacc tctcagcaaa agggctcatt gatggttgcc acgattctcg tgggcggcgt
     5761 cctcgccatc ctcacctttt atcgggctga cgctcgccga ccggcgcaac cccaaccgct
     5821 gggacgacaa ggcctacgag caggcgctcc gcagcgccgc tcgccgcaca cctggtagcc
     5881 gccttccgtc ggggccgtcc acatcgtgct ggcgctgacg ggctgcacca gccggcaatg
     5941 aggagcgggt tgtcccggta tccttgtcga cgaagatcac gagctgggtg gggcctcgtc
     6001 gatggtcggc tcgttctcca actcgccggc gggtttcttg agcccgctgt caccaagtcc
     6061 aggccgcagc gatgactcat gcggcacgag ccggtagggc tcctgcctct tgccgctgac
     6121 cgagcagtgc gggtctggtc gccacgggtc gatgacgtcg atcccggtgt ctcggaaatc
     6181 cttgaggttg cgcgtggcca aagctgcctg gaggacgcgg cagatggcgg cgatctgcgc
     6241 gtcgaagcca tcgatcgggc ggcccgcacg gtcccggccg ctcaccaccg tcgcgtatgc
     6301 cagcgccgcc tttgcgtcga acgaaaggat gcgctcctcg aaggccgcga acacctcctc
     6361 ggcggcggat cgcagcaacc gcttccgcgg gccgccgggc agccgctcga tgccataggc
     6421 gatctcggcc agtgtgatcg acgtcgtgta cagctcgcgc tcgttgcctc gcacccaggc
     6481 gaccaccgcc gctgtcggcg acggccgcat gagctccgag accacgttgg tgtcgacgac
     6541 gatcattctg gaaggtccgc cgcacgcgcc ggtaccgaac gtgccggcag gtcgagttcc
     6601 accccgccaa gcgccccaaa tcggtccaga agggtcgtga acagtccttc agaccgctcc
     6661 ggctccctga ccgcctcctg gaggatggcg cggatctcgg actccatcga ccgcccgtgc
     6721 tgggcagccc tgacgcgcag aaggcgcttt gtctcttcgt ccaggtttcg gatgctgact
     6781 gctgccatca tgacccccct cctatgctag caatgctagc accgccgagc gttggaccgt
     6841 gagtcacgcg agtcgtgcct ggccgatcaa gcgtcgcggc agcggaggac cacctgctcg
     6901 ccaagctcgg cgaccgcgac cgcacccagc tcgtcatcgt ggcctacgag accgggctgg
     6961 tcgccccggc ctcctcttgc tctatgcagc agccgcgcct acccgtcgcc gctggccacg
     7021 acccggacgg tctcgattcc ctgccatctg gcgtcgccgg tcaggatgag gtcggcctcg
     7081 tggcgcagcc cggtggccac gatcaccgcg tcggggagct tgaggcctcg cttggctcgc
     7141 agttcggcgg cgagccgggc gatgctcgcc gagacgtcca ggatgcgact ctgggcggcg
     7201 aagcgctcga cgatctggac cgcatcccct cccgctcgca ggggtccgac gagaagctcg
     7261 gcgtaggtga gcgccgagat gccgagtgcg tgccggtccc gctcggcgag caggacgcgg
     7321 gcggcggccc ggtggagggc gtcgtggcga ttgagggcgg cgatgacgat cgaggtgtcg
     7381 agcagcgcta gtcccattca gcgcgcaact cctggaggta accctccggg tagacgtcgc
     7441 tgccgagcgc gccgagcagg tcttcgagcc ggtcgtcggc gcgggtgagg atgaccctgc
     7501 cgggaccgag cgcctggacc cggagcgact ccccggcctg gattcctgcc tcggcgagga
     7561 ccgcgacggg aacggttacc tggtgcttgc ggctcaggcg ggtgaacccc cgacgccgag
     7621 gaggtttctt ttctttctcg ctcatgagta aagcttacca tcaccgagtc gccctaggcc
     7681 gaaatgctag cctcgtccgc tagcacctag atgtcgcccg ctggtgagcc tgggtagcgg
     7741 tggagggcgg agccgccgcg gaaggaggcg aagacgacgc agccttcggg gtattcgaag
     7801 gggccgtggg ggacctcggg ggggccgtag caggagtcgc cggggtggag ggggtcgccg
     7861 ttggcgatct ggaggccttc gaggacgacc acggagtgct cggaggcgtg ggcgtgctcg
     7921 ggttcgacgt agccgggggg gaacttgatg agcatgtcgg tgtgaccacg ctcggggtcg
     7981 tggtggtaga ccttgacctg gacgccgtgg ccgagggcga ggacctcgct gtcgtcctgc
     8041 caggcgaggt cccagcggtg cgcggtgtgg aactccttgc ccatgggttg ttctcctaac
     8101 tgacgtcgag ggcgaccttg agctgggacg gctcccggca ggcggcgaag gcgctggggg
     8161 cctcctccag cgggaaggtg gcggtgagca gcggctccag ccgcagcgcg ccggcggcgc
     8221 agagctggat ggcgcggtcg tagtcgcgcg ggcgggcggc gcgcgggttc agcagggtca
     8281 gctccttgta gtagagctgg taggtgggca gccgctcggc gatcgtggtg gtgccgaaca
     8341 ccaggatggt ggcgcccgac ccggccagct cgatcgactg ggccagggtg tcggcggtgc
     8401 cggcggcctc caccaccagg tcggcgccac ggccgccggt ggcctcggcg accacgctgg
     8461 cggcctcgcc gggggaggcg gtggcgctgg cgccgagctt ggcggccagc tcgcgcttgg
     8521 cggcggagcg ggtgacgccg acgatcgtgg tgatcccgcg cgctcgcagc agctgcaggt
     8581 ggagctgccc ggcgacaccg aggccgacga ccaccgcgct ctggccgggg aagacctcca
     8641 gcatggtctc cgcgtggacg caggtggcga gcacctgcag cagcgcggcg gcgtcgtcgc
     8701 cgatcgtctg cggcagcggg tggagctgca gctcgtcgac ggcgaccagc tcggcgaagc
     8761 cgccgtcggt gtcgcggccc atgaggccgc cctggcggca gaggtagccg cggtcacgca
     8821 ggcacagctc gcagtggccg caggcgatcc ccgggtcgac cagcacgcgg ctgccctcgg
     8881 ggacgaggcc gcgggccccg gcggcgagca cggtgccgac catctcgtgc ccgatgatca
     8941 gcgggacccg cactgggatc tggccgctgg cgatcttcag gtcggtgccg cagaggccgg
     9001 cccgccgcac ccgcagcagg gcgcgcccgc cggggtcggg ctcaggcacc tcctcgacgc
     9061 tgaccgcgtt gatatctgtg agcagcaccg ctcgcatgcg accaccacgc tcccctgccc
     9121 ccacgctcac cgtgcgacat tttgcaatat gtaagacggt tggccgagcc gtcaagccgg
     9181 tggccgggtc aaaatcagtc aggtcggtca ggcggccggc tgggcgacca gcccggcggc
     9241 ctcgatgccc gcgagggcgc acagctcgtc gttgtcggag ttgtcgccgg tcacgccgac
     9301 cgcgccgagc agctccccct cgggcgagcg caccagcacc ccgcccggcg ccggcagcat
     9361 gcggccgccg gagacctcga agagcgcact gaagaagacc ggggcgtcgg cggcgcggcg
     9421 ccccacctcc cgcgacccgt agcccatgcc gagcgctccc cacgccttgc cgacggcgat
     9481 ctgcggccgc aggatcccgg agccgtcctc acgcttgagg cagagcgggt ggccgccggg
     9541 gtcgagcacc gcgatggtga ggggctcgaa cccccgctcg cggccacgtg ccaggcccgc
     9601 gtcgatgatg atggcggcct gctgcagggt gagcgtggtc atggatgccg gtcgctcctt
     9661 agtcgtcgag cagggcgacc gcgcgggtgg gcccgccgtg gccgccgcgg atgcgcagcg
     9721 ggaacccggc gaaggtgaag cggcgcccgc tcaccttgtc gaggttggcc aggttctcgt
     9781 agtgggtgat gtgctcggcg cggcacatca tatggcaggg gtagctgcgg ctcgtcgggt
     9841 tgtcgggggt gggcgagtcg acgccgaagg tcttcacgcc gcggtccacc agccactggg
     9901 acccggccgc gccgaggccc gggaagctgg tcaggtactc ccgggtgcca tggtagcggt
     9961 tccaggtggc ggtgtagagc agcaggatgt cgccgcgccg cagctccagg ccggagatct
    10021 ccagggcggc ctccagctgg gcgggctcga cgtcggtccg tggccggcag gcggagacgt
    10081 ccaggcagat cgcgtcgccg tagaacagct ccagcggcat ctggtcgatg ctggccgcct
    10141 ccgggtcggg gtcgaggtgg ctgaaggagt cgacgtgggt ggggccgttg tcgttgacca
    10201 gcaggccggt ggtctggaac gagaagccgc cctcgaagcg ctcgctggtc tcctcgtggg
    10261 aggcgtggtc gaacaccacc gtcttgaggt ggcccgggta gacgtacatt ccggtgtaga
    10321 tctcctgtga gaggtcgatc agcctgggca tcgcctgctc ctgctccccc gatgacgggc
    10381 tccccctgac aaagtcgcct ggggattctc gcacctcatg agaattctcc aaaatgcatt
    10441 gtgcaatgct ctctacgtcc gccgttcgca gcacaggagc cgccgtgatg accgaccagc
    10501 ctgaccagat gctgatcgaa tgggacgtgc ccatcgagat ggacgacggc gtcgtgctgc
    10561 gcgccgacgt cttccgcccg cctcctcagg aggagggcca gcgccgcgac ccggtgctgc
    10621 tgagctacgg cccctacgcc aagggcctcg ccttccagga cggctacccg gagcagtggg
    10681 cggccatgtg ccgcgagcac cccgacgtcc ccgccggctc ctccaaccgc taccagaact
    10741 gggaaaccat cgaccctgag aaatgggtac ccgccgggta cgcctgcgtc cgggtcgacg
    10801 cacgcggcgc cggccgctcc cccgggtacc tggaccccct ctcgccccgc gagacccggg
    10861 atctctacca ctgcgtcgaa tgggccggga cgcgggagtg gagcaacggc aaggtgggcc
    10921 tcgccgggat ctcctactac gcgatcaacc agtggcacgt ggccgcgctg cagccgccgc
    10981 acctggccgc catctgcccc tgggaggggg cggccgactg gtaccgcgac atgacccacc
    11041 acggcggcat cgcctgcacc ttctgggcga actggtaccg ccagcaggtc ctcggcgtgc
    11101 agcacggcgt cggcgacaag ggcccgtcca gccgcgtcac cggcgagccg gtcgccggcc
    11161 cggagacgct cgacaccgag gaactggagc gccgccgcac cgacctggag gccgagatca
    11221 tcgcccaccc gctcgacgac gcctaccacc gggagcgctc ccccgactgg tccagggtca
    11281 ccgccccgct gctgtcggcg gccaactggg gcggccaggg cctccacctg cgcggcaaca
    11341 tcgaggggtt cacgcaggca gcctcccgca ggaaatggct ggaggtccac ggcctggagc
    11401 actggaccca cttctccacc gactacggcc gcggcctcca gcggcgcttc ttcgaccact
    11461 tcctgaaggg cgaggccaac ggctggaact tccagccgcc ggtgctgctg caggtccgcc
    11521 acgccgacgg cagcttcacg cagcgggcgg aacgcgcctg gccgctggag cgcacccgct
    11581 gggcgcgcct ctacctggac ccgtcggccg ggcgtgcggg cgaacgaggt gacgccgtcc
    11641 acgggacgcc gccagccgag cgtgcgggca agcgaagtac tgttgtccac gggaggctgg
    11701 cggaggcgga ggcggaggcc ggtcacagcc tggccgcgcg gctgccgctc acatcggcgc
    11761 gggccacgtt cgcggcgctc ggcgacggga tcaccttcac cactccgccg ctggcggcgg
    11821 agaccgagat caccgggccg gtcgcggcca agctgttcgt gtcctcatcg accagcgacg
    11881 ccgacctgtt cgtggtgctg cgggtgctgg acccagaggg ccgtgaggtg accttccagg
    11941 gggcgatcga cccgcacacc ccggtcgcgc agggctggct gcgggcctcg cgacgcaagc
    12001 tcgacccggc actgagcctc ccccaccgcc cctggcacac ccacgacgag caccagccgc
    12061 tggtgccgga cgaagtctat gagctggaca tcgaaatctg gccgacctgc gtggtggtcc
    12121 cggccgggta ccgcctcgcg ctgacggtgc gcggccgcga ctacgagtac gaagacgccg
    12181 gcgg
//
LOCUS       DASWDC010000211         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_54088, whole genome shotgun sequence.
ACCESSION   DASWDC010000211 DASWDC010000000
VERSION     DASWDC010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54088"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VF995_06855"
     CDS             complement(<1..207)
                     /locus_tag="VF995_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437591.1"
                     /note="Involved in peptide bond synthesis; alters the
                     affinity of the ribosome for aminoacyl-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX9343317.1"
                     /translation="MPTTNDLKNGMTLNIEGNLMSVVGFQHVNPGKGSAFVRTKLKNV
                     RTGRVLDKTFRADEPVEVAILEKRE"
     gene            complement(296..1405)
                     /locus_tag="VF995_06860"
     CDS             complement(296..1405)
                     /locus_tag="VF995_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX9343318.1"
                     /translation="MSVTDQGAHGAHQRAARRLALRNLLAQADLAALLVTSPANLRYL
                     TGFDGSYGAALIRQDHTVFFFTDRRYDQMVSRLLPDAEVVLAPGGDLVGAVAGVLGVD
                     GEGLGFEPAGLTWGEGQRLRHALPNQAVVPAPPLVEELREVKDTDELAAIARAAQLGS
                     DALAELLPTLREGVTERALAFELELAMRRRGALRLAFDSIVAFGEQAAEPHHHPGDRP
                     LARGDLVKLDFGAEVDGYCSDMTRTVVFGRASERQRELYELVRRAQAAGLASLRAGVT
                     GDQVDRACREVITEAGYGDAFSHPVGHGVGLEIHEAPRMRAGASGRIGAGTPVTVEPG
                     VYLPGFGGIRIEDLAVVREDGHELLTTAPKDLLEL"
     gene            complement(1402..>1980)
                     /locus_tag="VF995_06865"
     CDS             complement(1402..>1980)
                     /locus_tag="VF995_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX9343319.1"
                     /translation="TLPAAELRSGMAEVIKAGLIADPALAAACATNASRAASGDLDAL
                     APLAEAAIRVKAAVVGADEREAGRRATLNYGHTLGHALERLAGYQGLRHGEAVALGMI
                     FAARVAEAAGLAAPGLADGHAALLRAAGLPVGGVDLDPDAVLDAMATDKKASGSGLRL
                     VLLREVGRAELLPAPPRAVLAEAIRSLAEEPR"
BASE COUNT          234 a          699 c          771 g          276 t
ORIGIN      
        1 ctcccgcttc tccaggattg ccacctcgac cggctcgtcg gcccggaagg tcttgtccag
       61 cacccggccg gtgcgcacgt tcttcagctt ggtgcgcacg aaggcgctcc ccttgccggg
      121 gttgacgtgc tgaaacccga cgacgctcat gaggttgccc tcgatgttca gcgtcatccc
      181 gttcttcagg tcattggtgg ttggcatgtt cgcctcctgc gccgaccgcc agcacagccg
      241 gggcggttgg ctcgagtcgt caggataccg tcagagtccg tgttgacagc gttggttaca
      301 gttcgaggag atctttgggt gcggtggtaa gcagctcgtg gccgtcctcg cggacgaccg
      361 cgagatcctc gatccggatc ccgccgaagc cgggaaggta gacacctggc tccacggtga
      421 cgggcgttcc ggcgccgatc ctaccagagg cgcccgcgcg catccggggc gcctcgtgga
      481 tctccagccc gaccccgtgg ccgaccgggt ggctgaaggc gtcgccgtag ccggcctcgg
      541 tgatcacctc gcggcaggca cggtcgacct ggtcgccggt caccccggcg cggaggctgg
      601 cgaggccggc ggcctgcgcc cggcggacca gctcgtacag ctcccgctgg cgctcggagg
      661 cgcggccgaa gaccaccgtg cgggtcatgt ccgagcagta gccgtcgacc tcggcgccga
      721 agtcgagctt gacgaggtcg ccgcgggcca gcggccggtc gcccgggtgg tggtgcggct
      781 cggcggcctg ctcgccgaag gcgacgatgc tgtcgaaggc gagccgcagg gcgccccggc
      841 ggcgcatcgc cagctccagc tcgaaggcca gcgcccgctc ggtgacccct tcccggaggg
      901 tggggagcag ctcggcgagg gcgtcgctgc ccagctgggc ggcccgcgcg atggcggcca
      961 gctcgtctgt gtccttgacc tcgcgcagct cctcgaccag cggcggcgcg ggcaccaccg
     1021 cctggttggg cagggcatgg cgcagccgct ggccctcgcc ccaggtgagc ccggccggtt
     1081 cgaagccgag gccctcgccg tccacgccga gcacgccggc gaccgcgccg accaggtcgc
     1141 cgccgggcgc cagcacgacc tcggcgtcgg gcagcagccg cgacaccatc tggtcgtagc
     1201 gccggtcggt gaagaagaag acggtgtggt cctggcggat cagcgccgcg ccgtagctgc
     1261 cgtcgaagcc ggtgaggtag cgcaggttgg cggggctggt caccagcagt gccgccaggt
     1321 cggcctgggc gagcaggttg cgcagcgcga ggcggcgcgc ggcgcgctgg tgggcgccgt
     1381 gggcgccctg gtcggtgacg ctcatcttgg ctcctccgcc agcgagcgga tcgcctcggc
     1441 cagcaccgcg cggggtgggg cgggcagcag ctccgccctc ccgacctcgc ggagcaggac
     1501 caggcggagg ccgcttccgc tggccttctt gtcggtggcc atcgcgtcca ggaccgcgtc
     1561 ggggtcgagg tccaccccgc cgacgggcag gccggcggcg cgcagcagcg cggcgtgccc
     1621 gtcggcgagg ccgggggcgg ccaggccggc ggcttcggcg acgcgggcgg cgaagatcat
     1681 gccaagggcg accgcctcgc cgtggcgcag gccctggtag ccggcgaggc gttccagcgc
     1741 gtggccgagg gtgtggccgt agttgagggt ggcgcggcgg ccggcctcgc gctcgtcggc
     1801 gccgacgacg gcggccttga cccggatggc ggcttcggcc agcggcgcga gcgcgtcgag
     1861 gtcgccgctg gccgcgcggc tggcgttggt ggcgcaggcg gcggccaggg ccgggtcggc
     1921 gatcagcccg gccttgatca cctcggccat gcccgagcgc agctcggcgg cgggcagggt
//
LOCUS       DASWDC010000212         7267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_111330, whole genome shotgun sequence.
ACCESSION   DASWDC010000212 DASWDC010000000
VERSION     DASWDC010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111330"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VF995_06870"
     CDS             complement(<1..368)
                     /locus_tag="VF995_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343320.1"
                     /translation="MIPAVRRHPLVVVVAALVVVVVDCECLVVVVVPVVDDGTVVAVV
                     VVVDGAVVVVGVVVIGGSVVGGSVVGGAVEGGGCGTPGRGCAGTTRTAPAKPRRQMVA
                     TRTTSPVWGACTIRPLPMYMP"
     gene            375..944
                     /locus_tag="VF995_06875"
     CDS             375..944
                     /locus_tag="VF995_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016827072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX9343321.1"
                     /translation="MERLWTPWRMAYIRGEIGGRVADSGCVFCELPARGDDQGNHILA
                     RGRHTFVLLNAFPYNPGHLMVAPFRHLGDFGALSADELAESMAFTKTAIRALEESSDA
                     HGFNLGMNLGTIAGAGIADHLHLHLVPRWGGDTNFMPVVGQTKVLPELLAETWERLRP
                     WFEQAETATEAESGARAEIQEVQEEGERG"
     gene            complement(941..1846)
                     /locus_tag="VF995_06880"
     CDS             complement(941..1846)
                     /locus_tag="VF995_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX9343322.1"
                     /translation="MTTIDLHTHTRASDGSDTPAELVEAAARAGVKVLAVTDHDTVAA
                     VAAAQAAGAEHGVRVLVGLEITGEVAGSAGLPWTASLRSPARSAGGRVVHLLAYGAGL
                     LAPGAVDRVEAVRRERLERNHRIGERLEALTGVGYDQAAALAGRSALSRAHFARALIQ
                     AGVVADTAEAFDRYLSNGRPAYAPAPSLPVEEVVALVHGLGGLVVLAHPGRLGGAEAD
                     GVVAAAIEAGIDGLEVWHSQHDADARHHWQATADRHGLLVTGGSDYHGAHKPTVRIGS
                     GTDDNVDVPDQVLADLEERLSAVAG"
     gene            complement(1870..2805)
                     /locus_tag="VF995_06885"
     CDS             complement(1870..2805)
                     /locus_tag="VF995_06885"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9343323.1"
                     /translation="MRWPAPLFVIGAPRSGTSLLYKSLCLHPTAAWISNWVGRYPSRP
                     RLAALNRAARHLPGARRRVWFGADGANAYVYGKPRPALERVFPMPIEGERVFNHCGIA
                     LPGPGSPPGGSPARNASGERSRAVDCLRASFRTLTRWSGGRVLVCKRIANNQRIPLLA
                     EAFPEARFVHLIRDGRAVAYSLSRVDWWDRDVVWWYGDTPVRWREAGNDPWELCAEHW
                     NRELASIGEGLAAVPAGQRLEVRYSELIADPIPGIERIAAFGGLGLDAGWHAELTRLS
                     YPNKDESWRKHLDDEARARVEKIQADTLRRLGLLE"
     gene            complement(3014..3286)
                     /locus_tag="VF995_06890"
     CDS             complement(3014..3286)
                     /locus_tag="VF995_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343324.1"
                     /translation="MAVIAVGSLVAALEVTAHAVDPNKDVLRPSSGIAPAAVYDKPRS
                     ATGSTTTTRPAAKSGIGSSVVVIIGQPSASTRTTRQAPATTATTAP"
     gene            3632..4510
                     /locus_tag="VF995_06895"
     CDS             3632..4510
                     /locus_tag="VF995_06895"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9343325.1"
                     /translation="MGSKRNVIRMGGNVLALATSRARLLPSFLVIGTKRGGSTSLYDY
                     VTRHPAVLPARVEKGSHYFDVNHGRGWTWFRSTFPLARGSDVITGEASPYYMFHPLAA
                     ERFAAALPEVRLIASLRDPVTRAWSQHQYETKRGFEDLPFEEALAREAERLAGQEERV
                     RAGEESFAHRHHTYLARGRYAEQLERIYQLVPPERVLVVRSEDMFADPAAALARIWRF
                     LGLADHQLEDVPVLKANRYEQGMPAAARAYLQSYYEPHNQRLYRLPGIDFRWEAEAPA
                     AAAGPPERLASLPSQP"
     gene            4582..5997
                     /locus_tag="VF995_06900"
     CDS             4582..5997
                     /locus_tag="VF995_06900"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX9343326.1"
                     /translation="MTKLVTLVAVFFAASMAAISGLAIDSGSKMLIVMPLAAAVGVAM
                     AVLALTRFSVYVALMLVLRSSLDIAKLSSSSAGNTATNSTSARGLDPSSLLAVLFLLM
                     AALWLAAQHHRQGGLPTATWTRRALLWFVAAAGLSALGSANVQSSALELLRVFATVMM
                     FIVLEQLLADHSAIKRIMVAIYCAMLFPLLFTAYGLASGHARTDSKGGYTRIAGTFQS
                     SNDFGRFLMFMLIMAAAIYPYLNRRWRKLLFLLMIPSYGFLMLTYTRTALLGTVFGLV
                     VVGIMQSKRLLLTLGVVAVCAVVAVPSLSSRFSSLGNSATAPGVTGPSGNSLAWRLSY
                     WSQVLPLANSNPVTGIGINMTQYNTDAAKQPHNDFIRAYVEMGILGFGAYVTMLVSLL
                     ALGRRAARAGPRGTLDRGIGVGFLGCAVAFVAVSAAANVMSNVVTLWYLFAFAAAASA
                     VVRLNTSNTPAAAKLVNDRRE"
     gene            6001..7005
                     /locus_tag="VF995_06905"
     CDS             6001..7005
                     /locus_tag="VF995_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX9343327.1"
                     /translation="MEISDYLRVIWRRLWILVLVPLLAGGIVTSMVLREPPKYTATAD
                     VAAPSVVGGGSGQYSGPNGIKAFVANFTAMVVSPQVVNQVSKTTKVSVDQINSGLSST
                     PVGQSGLVDVTYTTTHKVSAGPVAKAAASATMTALFQGQVTLANSGLTAASKTLAAAQ
                     KKLTDFRTLHGLAIDPNTQFQQDQSQRATAENGRAQASAAAHSTTPFDQQIAALNADI
                     AKLAPLLQPYTQLNNDVTAAQAAVQAATTTLQQASQVAQAASPTTTVSLNATKAASLL
                     PELIKKAAPAVGAGLFLAVLIVMMVELVARRPRPPGDPRYASTNSHSRVTSSSTVYRG
                     "
BASE COUNT         1035 a         2661 c         2533 g         1038 t
ORIGIN      
        1 ggcatgtaca tcggcaacgg cctgatggtg cacgcccccc acaccggcga cgtggtccga
       61 gtcgccacca tctggcgcct cggcttcgcc ggggcggtgc gggtggtccc ggcgcagccc
      121 cggccgggcg tgccgcagcc cccgccctcc accgccccgc cgaccacgct cccgccgacc
      181 acgctcccgc cgatgacgac caccccgacg accaccacgg cgccgtccac gaccacgacc
      241 accgccacga cggtgccgtc gtcgaccacc gggaccacga ccaccaccaa gcattcgcag
      301 tcgaccacca ccacgaccag ggcggcgacc acgaccacca aggggtgacg gcggaccgcc
      361 ggtatcatgc cggcatggag cggctctgga ccccctggcg gatggcctac atccgcggtg
      421 agatcggcgg ccgggtcgcg gacagcggct gcgtcttctg tgagctgccg gcccgcggcg
      481 acgaccaggg caaccacatc ctggcccgtg gccggcacac cttcgtgctg ctgaacgcct
      541 ttccctacaa ccccgggcac ctgatggtcg cgcccttccg ccacctgggc gacttcggcg
      601 ccctgagcgc cgacgagctg gccgagagca tggccttcac caagaccgcc atccgggcgc
      661 tggaggagag ctccgacgcg cacggcttca acctcggcat gaacctcggc acgatcgcgg
      721 gcgccggcat cgccgaccac ctgcacctgc acctggtacc ccgctggggc ggcgacacca
      781 acttcatgcc ggtggtcggc cagaccaagg tcctgccgga gctgctcgcc gagacctggg
      841 agcgcctgcg gccctggttc gagcaggccg agacggcgac cgaggccgag tccggcgcca
      901 gggccgagat ccaggaggtt caggaggagg gcgagcgggg ctagccagcg accgcgctca
      961 gccgctcctc caggtcggcg aggacctggt cggggacgtc gacgttgtcg tcggtgccgc
     1021 tgccgatgcg gaccgtgggc ttgtgggcgc cgtggtagtc ggagccgccg gtgaccagga
     1081 ggccatggcg gtcggcggtc gcctgccagt ggtggcgggc gtcggcgtca tgctgggagt
     1141 gccacacctc caggccgtcg atgccggcct cgatcgcggc ggcgaccacg ccgtcggcct
     1201 cggcgccgcc gaggcggccg gggtgggcga gcaccaccag cccgccgagc ccgtggacca
     1261 gcgcgacgac ctcctcgacc ggcagcgacg gcgctggcgc gtacgccggg cggccgttgg
     1321 agaggtagcg gtcgaacgcc tcggcggtgt cggcgaccac gccggcctgg atcagcgcgc
     1381 gggcgaagtg ggcgcgggac agggccgagc ggcccgccag cgccgccgcc tggtcgtagc
     1441 cgacgccggt gagcgcctcc agccgctcgc cgatgcggtg gttgcgctcc agccgctcgc
     1501 ggcgcaccgc ctccacccgg tcgaccgcgc cgggggcgag caggcccgcg ccgtaggcga
     1561 gcaggtgcac cacccggccg ccggccgagc gtgcaggcga gcgaagcgat gctgtccacg
     1621 ggaggccggc ggagccggcg acctcgccgg tgatctccag gcccacaagg acgcggacgc
     1681 cgtgttccgc gccggcggcc tgcgccgccg ccaccgcggc gacggtgtcg tgatcggtca
     1741 ccgcgagcac cttgacgccg gcgcgggcgg cggcctcgac cagctccgcg ggagtgtcgc
     1801 tgccgtcgct ggcgcgggtg tgggtgtgga ggtcgatggt ggtcatcggt ggcctttctt
     1861 tcgttgggct cattccagca ggccgaggcg ccgcagcgtg tcggcctgga tcttctcgac
     1921 ccgggcgcgg gcctcgtcgt cgaggtgctt gcgccaggat tcgtccttgt tggggtagct
     1981 cagccgggtc agctcggcgt gccagccggc gtccagtccc aggccgccga aggcggcgat
     2041 ccgctcgatg ccggggatcg ggtcggcgat cagctcgctg tagcggacct ccagccgctg
     2101 gccggcgggg accgcggcga gtccctcgcc gatcgaggcc agctcgcggt tccagtgttc
     2161 ggcgcacagc tcccaggggt cgttgccggc ctcgcgccag cggaccgggg tgtccccgta
     2221 ccaccagacc acgtccctgt cccaccagtc gacccgcgac agcgagtagg cgaccgcgcg
     2281 cccgtcgcgg atcaggtgca cgaagcgggc ctcggggaac gcctcggcca gcagcgggat
     2341 gcgctggttg ttggcgatcc gcttgcagac cagtacgcgc cctcccgacc agcgggtgag
     2401 ggtccgaaac gacgcccgca ggcagtcgac cgcgcgggac cgctcgccgc tggcgttcct
     2461 ggccgggctg ccgccgggtg gcgatcccgg gcccggcagg gcgatcccgc agtggttgaa
     2521 gacccgctcg ccctcgatcg gcatcgggaa gacccgctcc agcgccgggc ggggcttgcc
     2581 gtagacgtag gcgttggcgc cgtcggcgcc gaaccagacc cgccgccgcg cgccgggcag
     2641 gtgccgcgcg gcgcggttga gcgcggccag ccgcgggcgg ctcgggtagc ggcccaccca
     2701 gttggagatc caggccgccg tcggatgcag gcacagcgac ttgtagagca ggctggtacc
     2761 cgagcgcggc gccccgatga cgaacagggg cgccggccac cgcatggtca cgagtcgttc
     2821 gtcctccccc caagccaacc gagccaaacg gccccccacc caaaacggcg tccagccaga
     2881 cggcggcgca cgcgttgatc ctcgcgttga tccttgtgag cggcggagtg taacagaggc
     2941 gcggttccgc ccaaggtcgg acttgtaacg gtttggtctc cgcttggtct cgtgctgccg
     3001 tcggggaggc gcgctacggc gccgtggtgg ccgtggtggc cggcgcctgg cgggtggtcc
     3061 gcgtcgaggc cgacggctgg ccgatgatca ccaccacgct gctgccgatc cccgacttcg
     3121 ccgccggcct ggtcgtcgtg gtcgagccgg tcgcggaccg cggcttgtcg tagaccgcgg
     3181 cgggggcgat cccgctgctg gggcgcagca cgtccttgtt ggggtcaacg gcgtgcgccg
     3241 tgacctcgag tgcggcgacc agcgaaccca cggcgatcac ggccatgagc aggcgccggc
     3301 gtcgccggtg gggccgcgga tggggggacc gccgtgccac cttgcctccc ggagggtctc
     3361 cggctgtgac gggggacacg ctaatttctc atgaaccctt cggtattctc aacttgagga
     3421 tgaatcgtcc tcgggggaag ctggtgccgg gaatgccccg ctcgacgttg agtggacgag
     3481 tgggcctacg cacattgcag gttgtcggct catgcgctct cccgggagca tgtcgtgcaa
     3541 gacgtccaga acggggctca gacccggaac tggcgtctcg tcgtggatgg caatcggaac
     3601 ggtgaccgag tcggggggcg aacaggcgca aatgggatcc aagaggaacg tgatccgcat
     3661 gggcggcaac gtgttggcgc tggcgaccag ccgcgcccgc ctgctgccaa gcttcctggt
     3721 catcggtacc aagcggggtg ggagcacctc gctgtacgac tacgtcaccc gccacccagc
     3781 ggtgctgccc gcccgcgtcg agaagggcag ccactatttc gatgtgaacc acggcagggg
     3841 atggacctgg ttccggtcga ccttccccct ggcgaggggc agtgacgtga tcaccggcga
     3901 ggccagcccc tactacatgt tccaccccct ggcggccgag cggttcgccg ccgcgctgcc
     3961 cgaggtgcgc ctgatcgcct cgctgcgcga cccggtcacc cgcgcctggt cgcagcacca
     4021 gtacgagacc aagcgcggct tcgaggacct ccccttcgag gaggccctgg cccgcgaagc
     4081 cgagcggctc gccggccagg aggagcgcgt ccgggcgggg gaggagagct tcgcccaccg
     4141 ccaccacacc tacctcgccc gcggccgcta cgccgagcag ctggagcgga tctaccagct
     4201 ggtcccgccc gaacgggtgc tggtggtgcg gagcgaggac atgttcgccg accccgccgc
     4261 cgccctggcg cggatctggc gcttcctcgg cctcgccgac caccagctcg aggacgtgcc
     4321 cgtgctcaag gcgaaccgct acgagcaggg catgcccgcc gccgcccgcg cctacctcca
     4381 gtcctactac gagccgcaca accagcggct gtaccggctg cccgggatcg acttccgctg
     4441 ggaggcggag gcgcccgcgg ccgcggccgg tccgccggag cggctcgcgt cgctgccgtc
     4501 gcaaccatag gagccgccgc ggtgtctgct gccaacccgc cgtttcgcca ggacctcgtc
     4561 aagctggggg gaccgggggg tgtgaccaag ctggtcacgc tcgtcgcggt cttcttcgcg
     4621 gcgagcatgg ccgcgatcag cggcctggcc atcgacagcg gatccaagat gctgatcgtg
     4681 atgccgctgg ccgccgcggt gggcgtggcg atggcggtgc tcgcgctcac ccgtttcagc
     4741 gtctacgtgg cgctcatgct ggtgctgcgc tcctcgctgg acatcgccaa gctgagcagc
     4801 agctcggccg gcaacaccgc caccaactcc acctccgccc gcggcctgga cccctccagc
     4861 ctgctggcgg tcctgttcct gctgatggcg gccctgtggc tcgccgcgca gcaccaccgc
     4921 cagggcggcc ttcccaccgc cacctggacg cggcgggcgc tgctgtggtt cgtggccgcg
     4981 gccgggctga gcgcgctcgg ctcggccaac gtccagtcga gcgccctgga gctgctgcgg
     5041 gtcttcgcga cggtgatgat gttcatcgtc ctggagcagc tgctggccga ccactcggcg
     5101 atcaagcgca tcatggtcgc catctactgc gcgatgctgt tcccgctgct gttcaccgcc
     5161 tacgggctgg cctccggcca tgcccgtacc gacagcaagg gtggctatac ccgcatcgcc
     5221 gggaccttcc agtcctccaa cgacttcggt cggttcctga tgttcatgct gatcatggcg
     5281 gcggcgatct acccgtacct gaaccggcgc tggcgcaagc tcctgttcct gctgatgatc
     5341 ccaagctacg gcttcctgat gctgacctac acccgcaccg cgctgctcgg cacggtcttc
     5401 ggcttggtcg tggtcggcat catgcagagc aagcgactgc tgctcaccct gggggtggtg
     5461 gccgtctgcg cggtggtggc ggtgccgtcg ctgtcgtcgc gtttcagctc cctcggcaac
     5521 agcgccaccg cgccaggggt caccgggcca tccgggaact cgctggcgtg gcggctcagc
     5581 tactggtcgc aagtgctgcc gctggccaac tccaacccgg tcaccggtat cggcatcaac
     5641 atgacccagt acaacaccga cgcggccaag cagcctcaca acgacttcat ccgcgcctat
     5701 gtagaaatgg gcatcctcgg cttcggcgcc tacgtcacga tgctcgtctc cctgttggcg
     5761 ctgggccggc gtgcggcccg cgccgggccg cgtggaacgc tcgaccgggg gatcggggtc
     5821 ggattcctcg gctgcgcggt cgccttcgtg gcggtcagcg ccgccgccaa cgtcatgtcc
     5881 aacgtcgtga cgctctggta cctcttcgcc ttcgcagcgg ccgcgtcggc ggtagtcagg
     5941 ttgaacacca gcaacacccc ggccgccgcg aagctcgtca acgatcggag ggaatagttc
     6001 gtggagatca gtgactacct acgggtgatc tggcggcgcc tgtggatcct ggtgctggtg
     6061 ccgctgctcg ccggtggcat cgtcacctcg atggtgctgc gggagccgcc gaagtacacc
     6121 gccaccgccg acgtcgccgc accatcggtg gtgggtggcg gctccggcca gtacagcggc
     6181 ccgaacggca tcaaggcgtt cgtggccaac ttcaccgcca tggtggtgtc gccgcaggtc
     6241 gtgaaccagg tgtccaagac caccaaggtg tcggtcgacc agatcaattc cggcctgtcc
     6301 tcgaccccgg tcggccagtc cggcctggtg gacgtgacct acaccaccac ccacaaggtc
     6361 agcgccggcc cggtcgccaa ggccgccgcc agcgccacca tgaccgccct gttccagggc
     6421 caggtcaccc tggccaacag cgggctcacc gcggccagca agaccctggc cgccgcccag
     6481 aagaagctca ccgacttccg caccctgcac ggcctggcca tcgaccccaa cacccagttc
     6541 cagcaggacc agtcgcagcg ggccaccgcc gagaacgggc gcgcgcaggc gagcgcggcg
     6601 gcgcacagca ccaccccctt cgaccagcag atcgccgccc tcaacgccga catcgccaag
     6661 ctggcgccgc tgctgcagcc ctacacccag ctcaacaacg acgtgaccgc cgcgcaggcc
     6721 gcggtccagg cggccaccac gaccctgcag caggcgtccc aggtcgcgca ggccgccagc
     6781 cccaccacga cggtgtcgct gaacgccacc aaggccgcct cgctgctgcc cgagctgatc
     6841 aagaaggccg cgccggcggt cggcgccggg ctgttcctgg ccgtgctgat cgtgatgatg
     6901 gtcgaactgg tggcgcgccg cccgcggccg cccggcgacc cgcgctacgc cagcaccaac
     6961 tcgcactccc gcgtgaccag cagctccacg gtgtaccgag ggtgatgcca tggactaccc
     7021 ggcgacgacg attctggtga ggccaatcgt ggatggctga tcgaccggct gcgtcgacca
     7081 ggacgaagga gcagcggaac gggacctcca gcagccgtcc cggaccgcgg aaggaggccg
     7141 cccgaccggg cgagaccgag cgtcaggtgc tcggcatggc ccggggaggc ggcctcaacc
     7201 tcttcggcgc ggtctgcagc caggctgccc agctcggcgt cacgctgctg gtcgggcgtg
     7261 cgctcgg
//
LOCUS       DASWDC010000213         7322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_111459, whole genome shotgun sequence.
ACCESSION   DASWDC010000213 DASWDC010000000
VERSION     DASWDC010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111459"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VF995_06910"
     CDS             <1..491
                     /locus_tag="VF995_06910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9343328.1"
                     /translation="RAAAVSNEARRRWRVTGTDLLATLAAALPVDLFGIGAERVHLDG
                     VRPRGDLPAGRLHAAMARRRVYLHPVRWTSLGLSLLEAMHLGMPVVALATTEVVEAVP
                     PDAGVISTRVDALARALRELGGDPERARVLGKHARGAAVARYGLGRFLADWDRLLEEV
                     TG"
     gene            491..1771
                     /locus_tag="VF995_06915"
     CDS             491..1771
                     /locus_tag="VF995_06915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9343329.1"
                     /translation="MRVAMVSEHASPLAVLGGEDAGGQNVHVAALSTALAERGVEVVI
                     HTRRDDPSLPDRVAMPTATVKGAASVTVEHVDAGPPEPLPKDELLPHMEAFGAELRRA
                     WSAEPADLVHAHFWMSGMAALAAARPLGIPVVQTFHALGVVKRRHQGDRDTSPPSRIR
                     EETAIARGADRIVATCSDEVFELIRLGAELQRVSVVPCGVDLGLFRPDGPAEPRDPDR
                     PRLVVVSRLVERKGIGNVITALAELPSAELVIAGGPAAERLDRDPEARRLRALAEELG
                     VADRVDFRGQMGHEQLPPLLRSADAVVCVPWYEPFGIVPLEAMACGAPVVASAVGGLV
                     DSVVDGATGLHVPPRRPDLIAAALAELLADPARRAAFGRAGARRVSRRYGWGRIAGRT
                     LDAYLQTTLERQAPAVAEQPHTGSGSRGGNRVGR"
     gene            1768..3333
                     /locus_tag="VF995_06920"
     CDS             1768..3333
                     /locus_tag="VF995_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019330123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX9343330.1"
                     /translation="MTSWRARRRPPPSGERPLLVVGDALLDRDLEGRVERLLPDEPVP
                     VVEEPVERHRPGGAALAATLASRLDGREVVLVTALGADERGALLAGLLADAGVALIDL
                     GLSGPTPEKARVRVRGRTLLRLDYGGRAARPGPLGPAGQDALAGAGGVLVADYGRGLA
                     AEPSVRAALAALAALPHRVPVVWDPHPKGAVPLPGARLVTPNQAEVAWFAATATPAKP
                     GATGRDGRRAGKLMEAERPRAASPEPEADGFRATDLAVLTARARALAGRWKAAGLAVT
                     LGSRGALLVGGEEPPLLVPAAPVHGADACGAGDRFASAAAGLLAEGALPSEAVTGGVA
                     AATAFVAEGGASGLRPGHEPRLTSPPARASRGARMAHAERVVAATRARGGTVVAAGGC
                     FDLVHAGHIAMLRAARQLGDSLVVFLNSDASVRRLKGLGRPLVPEADRAAVLAALGCV
                     DAVVVFDESTPERVLERLRPDVFAKGGDYALADLPEAQVVAAWGGQAVVLPYLEGRST
                     SGLLKEVVRRGSG"
     gene            3320..4036
                     /locus_tag="VF995_06925"
     CDS             3320..4036
                     /locus_tag="VF995_06925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9343331.1"
                     /translation="MGVDSGSRSARTDPGTVVVTGGASGLGAAVAQAVAEAGGTPVVL
                     DLGEPPFPADHQRVDLADGRAAEAAVSAAAERHGGLDAVVTAAGVDRCGQLAEVPAKD
                     WERVIQVNLLGTAAVVRAAWPFLERSGGRVVTVASTLGLRALPDATAYCASKFGVVGF
                     TRALAAEAAGKVGVTMLVPGGMRTAFFDDRPEQYRPPADANLNRPDDVASAVLFALGQ
                     PPGCELRELVICPSTEPSWP"
     gene            4027..5169
                     /locus_tag="VF995_06930"
     CDS             4027..5169
                     /locus_tag="VF995_06930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9343332.1"
                     /translation="MAIGFTPRSNPDPNSNPNSDSDSDASFFSSLGDGSTLVANANAN
                     ANANGRDDNGGPPAVVILRAIGLGDLLTVVPALRALADAFPRHPRLLAGPPALAPLAR
                     CTGAVDELIPTSGLDEPPHRRLHGAGIAVNLHGSGPDSHNLLLAAEPRRLVAFAHPAV
                     PASSEGPRWRADEHEVLRWCRLLEENGIAADPTRLDLELPPGPLPYGARGATLVHPGA
                     GSPARRWPPRRFAAVARAEIDLGRELMITGGLTEVELAHEVARFAGLPETAVWIGNGG
                     VLALARMVQAADRVICGDTGVAHLATALGTPSVVLFGPVSPALWGPPPDRPWNKPLWA
                     GTSGDPSGQLPDPGLLSLSVDDVLEALDTLPPAPHRQVPAASGAPR"
     gene            complement(5199..5780)
                     /locus_tag="VF995_06935"
     CDS             complement(5199..5780)
                     /locus_tag="VF995_06935"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX9343333.1"
                     /translation="MRIVLLGPPGSGKGTQAVRLADQFGCADIATGDIFRANVAEGTP
                     LGRIAQEYMDRGDLVPDEVVIAMVTERLAEPDCQNGFLLDGFPRTVAQAEALDRELHR
                     LDSPLDAVLCFDVTEEELLKRLKGRAEQLHRSDDSEHTIRHRLEIYATTTRLLVEYYT
                     HRGLISMVDAIGQIEEITDRILLALGAATVQLA"
     gene            5902..6822
                     /locus_tag="VF995_06940"
     CDS             5902..6822
                     /locus_tag="VF995_06940"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9343334.1"
                     /translation="MLPQIQAFLEVARRQNLSRAAEALYVSQPTLTARLQALEAELGE
                     QLFVRTRRGMRLTEAGEAFLPFAEHAVQALDDGRERLAELRRGEVGKLLVGAPPTVST
                     YALPGLLARFTAAHPGVRLTVKTGTSEEILEMVLHDLVQVGLMRALGHPEVESMPVYS
                     DAVLLIAGPRHRLASRRVRLEELAGETLVLFGRSSSYRTFTTAIFKQAGLIPNSVLEL
                     DNIEAAKKMVEHGLGISLVPHSAITGELAAGTLALVDLIDANLPVREILAVRRRDAGP
                     PSGAVAVFLEMLAEVSDLVPAQAPTPALDT"
     gene            6851..>7322
                     /locus_tag="VF995_06945"
     CDS             6851..>7322
                     /locus_tag="VF995_06945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9343335.1"
                     /translation="MTRPPPPPPRPLVRPAEPGDLAAVRAIAAAYGNLRDWPQRPDYL
                     DHELASGRLLVATDELGEEGVVAFAGAYERGGVTHLGDLFVRPGLTGRGLGRRLLEAI
                     LPVDGELVTFASADPAAVALYCRFGMQPLAPLLYLRADPARPVAARLAASAAARA"
BASE COUNT          845 a         2738 c         2792 g          947 t
ORIGIN      
        1 cccgcgcggc ggcggtcagc aacgaggcgc ggcggcgctg gcgggtcacg ggcaccgacc
       61 tgctggcgac gctcgcggcg gcactgccgg tggacctgtt cggcatcggc gccgagcggg
      121 tccatctcga tggggtgcgg ccgcgcggcg acctgccggc cggccgcctc cacgccgcga
      181 tggctcggcg gcgggtctac ctgcacccgg tgcgctggac ctcgctcggc ctctccctgc
      241 tggaggcgat gcacctcggc atgccggtgg tggcgctggc caccaccgag gtggtcgagg
      301 cggtgccgcc cgacgccggg gtgatctcga cccgcgtcga cgcgctggcc cgggcattgc
      361 gcgagctggg cggcgatccg gagcgggcgc gcgtgctcgg caagcacgcc aggggagccg
      421 cggtggcccg ctacggcctg ggacgcttcc tcgccgactg ggaccggctg ctcgaagagg
      481 tgaccggatg atgcgggtgg cgatggtcag cgagcacgca agcccgctgg cggtgctcgg
      541 cggcgaggac gcgggcggcc agaacgtcca cgtcgccgcc ctctccacgg cgctcgccga
      601 gcggggcgtg gaggtggtca tccacacccg ccgcgacgac ccctcccttc ccgaccgggt
      661 ggccatgccc acggccacgg tgaagggcgc ggccagcgtg accgtggagc acgtggacgc
      721 cgggccgccg gagccgctgc ccaaggacga gctgctgccg cacatggagg cgttcggcgc
      781 ggagctgcgc cgcgcctgga gcgcggagcc agcggacctc gtccacgcgc atttctggat
      841 gtcggggatg gccgcgctgg cggcggcgcg gccgctcggg atcccggtgg tgcagacctt
      901 ccacgcgctt ggcgtggtca agcggcgcca tcagggcgac agggacacca gcccgccctc
      961 ccgcatccgc gaggagaccg ccatcgcccg cggcgccgac cggatcgtcg cgacctgctc
     1021 cgacgaggtg ttcgagctga tccgcctggg cgccgagctg caacgcgtct cggtggtgcc
     1081 gtgcggcgtc gacctcgggc tgttccggcc ggacgggcca gccgagccgc gcgaccccga
     1141 ccggccccgg ctggtggtgg tgagcaggct ggtcgagcgc aagggcatcg gcaacgtcat
     1201 caccgccctg gccgagctgc ccagcgccga gctggtgatc gccggcgggc cggcggccga
     1261 gcggctggac cgcgacccgg aggcccggcg cctgcgggcg ctcgccgagg agctgggcgt
     1321 cgccgaccgg gtcgacttcc gtggccagat gggccacgag cagctcccgc cgctgctccg
     1381 ctccgccgac gcggtggtgt gcgtgccgtg gtatgagccg ttcgggatcg tcccgctgga
     1441 ggcgatggcc tgcggcgcgc cggtggtcgc ctcggcggtc ggcgggctgg tcgactcggt
     1501 ggtcgacggg gccaccggcc tgcacgtccc gccgcgccgc cccgacctga tcgccgccgc
     1561 gctggcggag ctgctcgccg acccggccag gcgtgccgcc ttcggccgcg ccggggcgcg
     1621 gcgggtgtcg cgccgctacg gctggggccg gatcgccggc cgcaccctgg acgcctacct
     1681 gcagaccacc ctggagcggc aggcgccggc cgtggccgaa cagccccaca ccgggagcgg
     1741 cagccgcggc ggaaaccggg tgggccgatg acctcgtgga gggcgcggcg gcggccaccg
     1801 cccagcgggg agcggcccct gctggtggtc ggcgacgcgc tgctggaccg cgacctggaa
     1861 gggcgggtcg agcggctgct gccggacgag ccggtgccgg tggtcgagga gccggtggaa
     1921 cgccaccggc ccggcggggc ggcgctggcc gcgacgctgg cgagccggct ggacggccgc
     1981 gaggtggtgc tggtgaccgc cctcggcgcg gacgagcgcg gggcgctgct cgccggtctg
     2041 ctggcggacg ccggggtcgc tctgatcgac ctggggctgt ccgggccgac gccggagaag
     2101 gcgcgagtcc gggtgcgcgg ccggacgctg ctgcgccttg actacggcgg ccgggcagcg
     2161 cgccccgggc cgcttggccc tgccgggcag gacgccctgg ccggcgccgg cggcgtgctg
     2221 gtggccgact acggccgcgg cctggccgcc gagccgtccg tccgtgcggc tctggcggcc
     2281 ttggcggcct tgccgcaccg tgtcccggtc gtctgggatc cgcaccccaa gggcgccgtg
     2341 ccgctgcccg gcgcccgcct ggtcacgcct aaccaggcgg aggtggcctg gttcgcggcc
     2401 accgccacgc ccgccaagcc tggcgcgacc ggccgggacg gacgccgggc tgggaagctg
     2461 atggaggccg agcggccgag ggcggccagc ccggagccgg aggcggacgg cttccgggcc
     2521 accgatctcg ccgtgctgac cgcgcgggcg cgggcgctgg ccgggcgctg gaaggccgcg
     2581 gggctggcgg tgacgctggg cagccggggg gcgctgctgg tcggcgggga ggagccgccg
     2641 ctgctggtgc cggccgcgcc ggtgcacggc gccgatgcct gcggggccgg ggaccgcttc
     2701 gccagcgccg ccgccgggct gctggcggag ggtgcgctgc cgtcggaggc ggtcaccggc
     2761 ggggtcgccg ccgccaccgc gttcgtggcc gaagggggcg ccagcgggct ccgccccggc
     2821 cacgagccgc gcctgaccag cccgcccgcg cgggcctcgc gcggggcgcg gatggcccac
     2881 gccgagcggg tggtcgcggc gacccgtgcc cggggcggga ccgtggtggc ggccggcggt
     2941 tgctttgatc tggtgcacgc gggtcatatc gcgatgttgc gggcggcgcg gcaacttggg
     3001 gactctctgg tagtctttct gaactcggat gcctcggtac gccggctcaa aggactcggc
     3061 aggccgctgg tacccgaggc cgaccgggcg gccgtgctcg cggcgctcgg ttgtgtcgac
     3121 gcggtggtcg tgttcgatga gagcactccg gagcgggtgc tcgaacgcct tcggccagac
     3181 gtgttcgcca aggggggcga ctacgcactc gccgatctgc cagaggcgca ggtggtagcc
     3241 gcctggggcg gccaggcagt ggtgctcccc tacctggagg gccgctcgac atccggactg
     3301 ctgaaggagg tcgtccgacg tgggagtgga tagcggcagc aggtccgccc ggaccgatcc
     3361 cggcactgtc gtggtgaccg gcggggcgtc cggcctgggc gcggcggtcg ctcaggcggt
     3421 cgccgaggcc ggcgggaccc cggtcgtgct cgacctgggg gagccgccgt tccccgccga
     3481 ccaccagcgg gtcgacctgg ccgacggccg cgccgccgag gccgccgtca gcgcggcggc
     3541 cgagcgccac ggcggcctgg acgcggtggt caccgccgcc ggggtcgacc gctgcgggca
     3601 gctcgccgag gtgcccgcca aggactggga gcgcgtgatc caggtgaacc tgctcggcac
     3661 cgccgcggtg gtgcgggccg cctggccgtt cctggagcga tccggcgggc gggtggtcac
     3721 ggtcgcctcc accctggggc tgcgggcact gcccgacgcg accgcctact gcgccagcaa
     3781 gttcggcgtg gtcggtttca cccgcgcgct cgccgccgag gccgccggaa aggtcggagt
     3841 taccatgctc gtccctggtg ggatgcgcac cgcgttcttc gatgaccgac ccgagcagta
     3901 ccggccaccc gcggacgcca acctcaaccg cccggacgac gtcgcctcgg cggtcctgtt
     3961 cgcgctcggc cagcccccag gctgcgagct gcgagaactg gtgatctgcc cgtcaacgga
     4021 gccgtcatgg ccataggttt cacccccaga tccaaccccg accccaactc caaccccaac
     4081 tccgactccg actccgacgc cagcttcttc agcagcctgg gcgacgggag caccctcgtc
     4141 gccaacgcca acgccaacgc caacgccaac ggccgggacg acaacggcgg cccgccggcg
     4201 gtggtgatcc tgcgcgccat cgggctcggc gacctgctca ccgtcgtgcc ggcgctgcgg
     4261 gcgctggccg atgccttccc gcgccacccg cgcctgctgg ccggcccgcc ggcgctggcg
     4321 ccgctggccc gctgcaccgg cgcggtcgac gagctgatcc ccaccagcgg cctcgacgag
     4381 ccgccgcacc gccgcctgca cggcgccggc atcgccgtga acctgcacgg cagcggcccg
     4441 gacagccaca acctgctgct cgccgccgag ccgcggcggc tggtcgcctt cgcccacccg
     4501 gcggtcccgg ccagctccga aggtccccgc tggcgcgccg acgagcacga ggtgctgcgc
     4561 tggtgccgcc tgctggagga gaacggcatc gccgccgacc cgacccgtct cgacctcgag
     4621 ttgccgccgg gcccgctccc ctacggcgcg cgcggcgcca ccctggtcca cccgggcgcg
     4681 ggcagccccg cgcgtcgctg gccgccgcgc cgcttcgcgg cggtcgcccg cgccgagatc
     4741 gacctcggcc gcgagctgat gatcaccggc ggcctcaccg aggtcgagct ggcccacgag
     4801 gtcgccaggt tcgccggcct gccggagacc gccgtgtgga tcggcaacgg cggcgtgctc
     4861 gcgctcgccc gcatggtgca ggccgccgac cgcgtcatct gcggcgacac cggcgtggcc
     4921 cacctcgcca ccgccctcgg caccccctcg gtggtgctgt tcggcccggt ctcccccgcc
     4981 ctctgggggc ccccgcccga ccgcccctgg aacaagcccc tgtgggccgg caccagcggc
     5041 gacccgtccg gccagctccc cgaccctggc ctgctgtccc tgtcggtgga cgacgtgctg
     5101 gaggcgctcg acaccctgcc gccggcaccc caccgccagg tcccggcggc cagcggcgca
     5161 ccgcgctgac gctgaggcga acgggcgctg ggagcggatc aggcgagctg gacggtggcg
     5221 gcgcccaggg cgagcaggat ccggtcggtg atctcctcga tctggccgat cgcgtcgacc
     5281 atggagatca gaccgcggtg ggtgtagtac tcgaccagca gccgggtggt ggtggcgtag
     5341 atctcgaggc ggtggcggat ggtgtgctcg ctgtcgtcgc tgcggtgcag ctgctcggcg
     5401 cggcccttga ggcgcttgag cagctcctcc tcggtgacgt cgaagcagag caccgcgtcg
     5461 agcgggctgt ccagccggtg cagctcccgg tcgagcgcct cggcctgggc gacggtgcgg
     5521 gggaagccgt ccagcaggaa gccgttctgg cagtccggct cggccaggcg ctccgtcacc
     5581 atggcgatca cgacctcgtc ggggaccagg tcgccgcgat ccatgtactc ctgagcgatc
     5641 ctgccgaggg gcgtgccctc ggcgacgttg gcccggaaga tgtcacccgt ggcgatgtcg
     5701 gcgcagccga actggtcggc gaggcgcacc gcctgggttc ccttgccgct cccgggcggg
     5761 ccaaggagca cgattcgcat gcagttcccc cggtccggcg acgttgacgg tgaggctagt
     5821 gacggtaggt ggtggcttgc catcagtcca atagttcgtt ttgattacca acatcggaat
     5881 tcccgatgaa cggaggcagg gttgctgccg cagatccagg cgttcctcga ggtggcccgg
     5941 cggcagaacc tgagccgcgc cgctgaggct ctctatgttt cccagccaac cctcaccgca
     6001 cgcctccagg cgctggaagc cgaactcggc gaacagttgt tcgtccgaac ccgccgcggg
     6061 atgcgcctga ccgaggcggg cgaggcgttc ctgccgttcg ccgagcacgc cgtccaggcc
     6121 ctcgacgacg gccgcgaacg cctcgcggag ctgcgccgcg gcgaagtcgg caagctgctg
     6181 gtgggcgccc cgccgacggt ctcgacctac gccctgccgg ggctgctggc ccgcttcacc
     6241 gccgcgcacc cgggggtgcg gctcaccgtc aagaccggca cctccgagga gatcctggag
     6301 atggtcctgc acgacctggt gcaggtcggc ctgatgcgcg ccctcggcca ccccgaggtc
     6361 gagagcatgc cggtctactc cgacgcggtg ctgctgatcg ccgggccgag gcaccggctc
     6421 gccagccgcc gggtgcggct ggaggagctg gccggcgaga cgctggtgct gttcggccgc
     6481 agctccagct accgcacctt caccaccgcg atcttcaaac aggccgggct gatccccaac
     6541 agcgtcctgg agctggacaa catcgaggcc gccaagaaga tggtcgagca cggcctcggc
     6601 atctccctgg tcccacacag cgcgatcacc ggcgagctgg ccgccggcac cctggcgctg
     6661 gtcgacctga tcgacgccaa cctcccggtc cgcgagatcc tggcggtgcg ccgccgcgac
     6721 gccggccccc cgtcgggcgc ggtggcggtg ttcctggaga tgctcgccga ggtcagcgac
     6781 ctggttccgg cgcaggcgcc gacgccggcg ctggacacgt gacgacctga gcgtgaggtg
     6841 aggtgaccgg gtgacccgtc cgcctccgcc gcccccgcgg ccgctggtgc ggccggcgga
     6901 accaggcgat ctcgccgccg tccgggcgat cgccgccgcc tacggcaacc tgcgcgactg
     6961 gccgcagcgg cccgactacc tcgaccacga gctggcctcc ggccgcctgc tggtagccac
     7021 cgacgagctg ggcgaggagg gggtggtcgc cttcgccggg gcctatgagc gcggcggcgt
     7081 cacccacctc ggcgacctgt tcgtgcggcc cggcctgacc ggcagggggc tcggccggag
     7141 gttgctggag gcgatcctgc ccgtcgacgg cgagctggtg accttcgcct ccgccgaccc
     7201 ggctgcggtg gcgctctact gccgcttcgg catgcagccg ctggcgccgc tgctgtacct
     7261 gcgggccgac ccggcccggc cggtcgccgc ccgcctggcc gccagcgccg ccgcgcgcgc
     7321 gc
//
LOCUS       DASWDC010000214         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1530542, whole genome shotgun sequence.
ACCESSION   DASWDC010000214 DASWDC010000000
VERSION     DASWDC010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530542"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..913)
                     /locus_tag="VF995_06950"
     CDS             complement(149..913)
                     /locus_tag="VF995_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9343336.1"
                     /translation="MSYVDALALVPAWNEAGRIGATVTALRGLPEVAEVLVVSDGSTD
                     ATAARALEAGAHCLELAHNHGKGDALNAGLAALMTRVADGATPEPRALLLADADLAGT
                     AAGLRALLAPVLDDEADLAVADLPPQPGAGGFGLAMGLARWGMERTTGRRLGEPLSGQ
                     RALRWSALPPLIPFARGFGVEVAMTVDALRAGLRVVEVEVDLRHAATGRDLAGIVHRG
                     RQASAIARELARRQAWRRNGHRSPQHPDGGERESSR"
     gene            complement(910..1884)
                     /locus_tag="VF995_06955"
     CDS             complement(910..1884)
                     /locus_tag="VF995_06955"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEX9343337.1"
                     /translation="MINLRYHIVSLVAVFLALALGVVMGSTVFKDSLSAAQRALTNEV
                     VNQAESLRQQKNQLDAENGDLRSFGTAVLPSLLRDKLRGRHVVLLDTDQVDNGTWQGV
                     RDTLQQLAGATVDGRITFSTGRMTLQSGGDRSALANLLGGDSADPASLRTTLLDGLTN
                     RLVASERLPTDDAGRARDRLTGLADTKFASLDLANRFKGGKTPFPEPGSLFVIIGPSD
                     GTPTLTPDQFLVPLADKLSNKSLQPVAGVEDWIGTSSWVVALRANRDVNQRVTSIDDV
                     DQVFGQYALVEALAAQLQNPPQPPGHYGTKAGSDRLLPKSATTSGGTS"
     gene            complement(1921..>3004)
                     /gene="steA"
                     /locus_tag="VF995_06960"
     CDS             complement(1921..>3004)
                     /gene="steA"
                     /locus_tag="VF995_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEX9343338.1"
                     /translation="GDIAVIDHLDVDRVAAETLIERHPAAVVNAAPSLSGRYPHPGPM
                     LLVDAGIPLLDASGPEVFALREGEPVRLDEHGDVWRGDTVIAHGEWLEPEDVRKRIEA
                     AKDNLAGALEDFTLNTLSYVRRDRDLLLEGLDIPQLETIMRDRHVLVVVRGHGYKKDL
                     STLRGYIHEFRPVLIGVDGGADALLEVGLKPDVILGDMDSVTNEALSCGAEVVVHAYP
                     DGRAPGKERVERLGLTPKILPGAGTSEDMAMLLAYEADCDLIVAVGSHASMVEFLDKG
                     RPGMASTFLVRLKVGTKLVDAKGVNRLYRQGVRRGDLLLLVVGALVAMLMVTFVSAPA
                     RAVWSLMWKDAWELLRIFIDRIVSLF"
BASE COUNT          413 a         1037 c         1137 g          417 t
ORIGIN      
        1 accagcccga gcccggcgac cagggtccgc ctgcggtagt tgacccgcga caggttggtg
       61 gcggcgagcg cggcgagcac ctgcggcgcc gcccagcgcc cgagcgccca cccggcgacc
      121 aggccgagga gcaccagcgg cacaggcatc atcgggaact ctccctctcc cccccatccg
      181 ggtgctgcgg gcttcggtgg ccgttgcgcc gccaggcctg gcggcgggcc agctcgcggg
      241 cgatcgcgct ggcctggcgg ccgcggtgca cgatcccggc gaggtcgcgg ccggtggcgg
      301 cgtggcgcag gtcgacctcg acctcgacga cccgcagccc cgcccgcagc gcgtcgacgg
      361 tcatggcgac ctcgaccccg aagccgcgcg cgaacgggat cagtggcggc agggccgacc
      421 agcgcagcgc ccgctggccg gagagcggct cgccgaggcg ccggccggtg gtccgctcca
      481 tcccccaccg cgccaggccc atggcgaggc cgaacccgcc ggcacccggc tgggggggca
      541 ggtcagccac ggccagatcg gcctcgtcgt cgagcacggg ggcaagcagc gcccgcagcc
      601 cagcggcggt gccggcgagg tcggcgtcag ccagcagcag cgcccgcggc tccggggtgg
      661 cgccgtcggc gacgcgggtc atcagcgccg ccaggcccgc gttgagggcg tcgcccttgc
      721 cgtggttgtg ggccagctcc aggcagtggg cgccggcctc cagggcgcgg gcggcggtcg
      781 cgtcggtgga gccgtcgctg accaccagca cctcggcgac ctcggggagc ccgcgcaggg
      841 cggtgacggt ggcgccgatc cgcccggcct cgttccaggc ggggaccagg gcgagggcat
      901 ccacgtagct cacgacgtcc ctccggaggt ggtggcggat ttgggcagca gccggtcgct
      961 gccggctttg gtgccgtagt ggccgggcgg ctgggggggg ttctgcagct gggcggcgag
     1021 cgcctcgacc agcgcgtact gcccgaagac ctggtcgacg tcgtcgatgc tggtgactcg
     1081 ctggttgaca tctctgttgg cgcgcagggc caccacccac gacgaggtcc cgatccagtc
     1141 ctcaacgccg gcgaccggct ggagggactt gttggagagc ttgtcggcca gcggcaccag
     1201 gaactggtcg ggggtgagcg tgggggtgcc gtctgacggt ccgatgatca cgaacagcga
     1261 gccgggctcc gggaacgggg tcttcccgcc cttgaaccgg ttcgccaggt cgaggctggc
     1321 gaacttggtg tcggccaggc cggtgagccg gtccctggcg cgaccggcgt cgtcggttgg
     1381 cagccgttcc gacgccacca gccggttggt gaggccgtcc agcagggtgg tgcgcaggct
     1441 ggcagggtcg gcgctgtcgc cgccgagcag gttggcgagc gcgctccggt cgccgccgct
     1501 ctgcagggtc atgcggccgg tcgagaaggt gatgcggccg tcgacggtgg cgccggcgag
     1561 ctgctgcagg gtgtccctga cgccctgcca ggtgccgttg tcgacctggt cggtgtcgag
     1621 cagcaccacg tggcggccgc ggagcttgtc gcgcagcagg ctcggcagca ccgcggtccc
     1681 gaagctccgc aggtcgccgt tctcggcgtc gagctggttc ttctgctgcc gcagcgactc
     1741 ggcctggttg acgacctcgt tggtgagcgc ccgctgcgcg gcgctgaggg aatccttgaa
     1801 gaccgtcgag cccatcacca cgccaagcgc caatgccaga aacacggcga ccagcgacac
     1861 gatgtggtat cgcaggttga tcatcgctgc ctcaccagga ccgaaaacga gggcaaaatg
     1921 ctagaaaagg gagacgatgc ggtcgatgaa gatccgcaaa agctcccaag catccttcca
     1981 catcagcgac cacaccgccc gggcgggcgc cgacacgaat gtcaccatca gcatagcgac
     2041 cagcgcgccg accaccagca gcagcaggtc gccgcggcgc accccctggc ggtagaggcg
     2101 gttcacgccc ttggcgtcca ccagcttggt gccgaccttg aggcggacca ggaaggtcga
     2161 ggccatcccg gggcggccct tgtcgaggaa ctcgaccatg ctcgcgtggc tgccgaccgc
     2221 gacgatcagg tcgcagtcgg cctcataggc gagcagcatg gccatgtcct cgctggtgcc
     2281 cgcgcccggc aggatcttgg gggtgagccc gagccgctcg acccgctcct tgccgggcgc
     2341 gcggccgtcg gggtaggcgt ggacgacgac ctcggcgccg cagctgagcg cctcgttggt
     2401 gaccgagtcc atgtcgccga ggatcacgtc gggtttgagt ccgacctcca gcagcgcgtc
     2461 ggcgccgccg tcgacgccga tcagcaccgg gcggaactcg tggatgtagc cgcgcagggt
     2521 ggacaggtcc ttcttgtagc cgtggccgcg caccaccacc agcacgtggc ggtcgcgcat
     2581 gatggtctca agctggggga tgtcgagccc ttccagcagc aggtcccggt cgcggcgcac
     2641 gtacgagagg gtgttgaggg tgaagtcctc gagcgcgccg gcgaggttgt ccttggcggc
     2701 ctcgatgcgc ttgcggacgt cctcgggctc cagccattcg ccgtgggcga tcacggtgtc
     2761 gccgcgccac acgtcgccgt gctcgtcgag gcggaccggc tcgccctcgc gcagcgcgaa
     2821 gacttcgggg ccgctcgcgt cgagcagcgg gatgccggcg tcgaccagca gcatcgggcc
     2881 ggggtggggg tagcggccgg agaggctcgg cgccgcgttg acgacggcgg cggggtggcg
     2941 ctcgatcagc gtctcggccg cgacccggtc gacgtcgagg tggtcgatta cggcgatgtc
     3001 gccg
//
LOCUS       DASWDC010000215         6587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_56051, whole genome shotgun sequence.
ACCESSION   DASWDC010000215 DASWDC010000000
VERSION     DASWDC010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56051"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VF995_06965"
     CDS             complement(<1..588)
                     /locus_tag="VF995_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9343339.1"
                     /translation="MIGFCLAAGEGTRLAPLTDRTAKPLLMVEGRPLIDIACAALLAA
                     GAQRVVVNAYHHAGQLAAHLAGRPGVEVVVEPELLGNAGGPANARRLGLLGVPGDAED
                     VLLTCADVIVDPADLRALAAALERHREAEVVVGLIAVNPSDPFRFRVEGEAADQIGWA
                     VPDQAGRCSSAGCYALRATVFDAIPPGPAEFVKALL"
     gene            complement(585..986)
                     /locus_tag="VF995_06970"
     CDS             complement(585..986)
                     /locus_tag="VF995_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343340.1"
                     /translation="MELPNDPPVSPTVRITVLLRLASEGYEDAFGRRPEWLTLPAVDL
                     AELRESVGAGRVEQLRRAIGLREGQTCGAGGAAGTVILSEHAHRGLLGLMGLAALPAD
                     VLVEETPEGPRYSVPDDWFIAAPAPSASSSP"
     gene            complement(1138..1719)
                     /locus_tag="VF995_06975"
     CDS             complement(1138..1719)
                     /locus_tag="VF995_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX9343341.1"
                     /translation="MSGQHASQPIAARVRHLEERLIQAGVLTDAQIDQALERFLATAS
                     PTNGARLAARAWVDRDFRELVLADANAALAELDLGGRGGEFLRVVANTATQHNVVVCT
                     LCSCYPLRLLGPSPSWYKSEAYRARVVREPREVLAEFGLRLPPEMGITVWDSSAEARY
                     VVLPRRPPATENLTEPDLARLVTRNGLIGTAEV"
     gene            complement(1716..2042)
                     /locus_tag="VF995_06980"
     CDS             complement(1716..2042)
                     /locus_tag="VF995_06980"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEX9343342.1"
                     /translation="MREAAVPPRPGPRFGPGQRVRTAVVDPPHHTRLPRYARGRVGVV
                     DRVQAAAPLPDDNARRIEPPRVEPVYTVRFAAADLWGQGSHTVTVDLWESYLEAVDVA
                     VEAEGA"
     gene            complement(2035..2310)
                     /locus_tag="VF995_06985"
     CDS             complement(2035..2310)
                     /locus_tag="VF995_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343343.1"
                     /translation="MARINDLGGLQGMGLVPIGDEEDIEPFHADWEARVFALQAVLGR
                     QGLFTTDEFRDAIERMPVREYLRAGYYERWLVALETLLRERGILPDA"
     gene            complement(2329..2732)
                     /gene="rnpB"
                     /locus_tag="VF995_06990"
     ncRNA           complement(2329..2732)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF995_06990"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2793..3245
                     /locus_tag="VF995_06995"
     CDS             2793..3245
                     /locus_tag="VF995_06995"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX9343344.1"
                     /translation="MVPLLAALFILVPLAELAVIITVAHSFGVLNTLALLLLFSIFGA
                     WLAKRQGWMAWRRLQLALDEGRLPTVEIVDGALILLAGALLFTPGFITDTIGLLLLFP
                     PTRAALRRVAPTLALRRMSRRSRIRVRDEYGTEVHWGRPQIDERDERR"
     gene            complement(3274..3759)
                     /locus_tag="VF995_07000"
     CDS             complement(3274..3759)
                     /locus_tag="VF995_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HEX9343345.1"
                     /translation="MITEPEAEPTLELDLEQERVEDVVIWADGGARGNPGPAGYGAVV
                     ETPDGRILAKLSQGIGWATNNVAEYKGVIAGLEHARVLGARRVKVRADSLLVVNQQRG
                     IWKVRNEDLQPLWAEVRRLVRGFDRVTFEHVPRERNCAADALANQAMDAQGKVRRPSG
                     R"
     gene            complement(3777..4505)
                     /locus_tag="VF995_07005"
     CDS             complement(3777..4505)
                     /locus_tag="VF995_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX9343346.1"
                     /translation="MDAPLEQQRALVELQGHDSAMDRLAHRRQNLPEHAKLEELTQAL
                     ATIDQLTAERQGTLATLSREQARLETDVEMVTRKAASEDARAVSGKVTSPKELTAIQE
                     EVASLHRRQATLEDELLEKMEQRETLEAELAELAGRREAVTAEQAKVTEARDAALAEI
                     ERELEVERAARAALAPGIGEELLRLYEQIRARYGGIGAGALVGNTCQGCRVSVSHVEL
                     AALRKLPPERVKRCENCRRILVIE"
     gene            complement(4543..6099)
                     /locus_tag="VF995_07010"
     CDS             complement(4543..6099)
                     /locus_tag="VF995_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020216637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX9343347.1"
                     /translation="MQVTFLGQAGLYVETDHGSILCDPWFNTAFFASWFPFPSNEDVD
                     LDAISHPDYLYVSHLHHDHFDPDFLGEQVDKRAIVLLPDYPMNHLEQELRALGFTRFV
                     KTRNSEPVELDGLRIMIMALVAPTDGPIGDSGLAVDDGTTKLFNQNDSRPIDLEPLRA
                     FGHFDAHFLQFSGAIWYPMVYDFPERMKQNLGHRKRQNEMARAKRYAEELGASFVFPS
                     AGPPCFLDPDLFGFNDIDRDPANTFPDQTVFLELLADSGMDNGRLLIPGSVAAVTPGS
                     CRVEHPGDDAVDAIFSDKLSYLRAYQQRQLPEIARRKASWPRHQIDIVSSLKAWWEPL
                     LAQADRTCAGVNGRVLLDATDPVEGEQVVIDFLDRRVDLWKGEPCRYTFRIDRALVEA
                     CIARHDEDWVNELFLSCRFEASRDGPFNEYVYNWFKVLSPERLQYAEGWYAELGAAEE
                     LVPMDGYLVQRRCPHLKADLAQFGVVEDGVLTCRMHGWQFDLATGRCLTSDDRRLHSV
                     KADEATDGVG"
     gene            complement(6199..>6587)
                     /locus_tag="VF995_07015"
     CDS             complement(6199..>6587)
                     /locus_tag="VF995_07015"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF020742.4"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX9343348.1"
                     /translation="ELGARELAAGQATLVRRDTRAKSPVPLDGAARAARGLLDQQQQA
                     LHADSLAFREANTHTVTDLDGFRQRIGGGGFFRAAWCGKPECEDALGEGTGASIRAIV
                     PDDPPAAACLVDGAPATAAVLVARAY"
BASE COUNT          895 a         2284 c         2416 g          992 t
ORIGIN      
        1 gaggagcgcc ttcacgaact ccgccggacc tggtgggatc gcgtcgaaga cggtcgcacg
       61 cagggcgtag cacccggccg acgagcaccg cccagcctgg tcgggcactg cccagccgat
      121 ctggtccgct gcttcgccct caacccggaa ccggaacgga tcactggggt tgacggcgat
      181 cagaccgacg acgacctcgg cctcgcgatg ccgctccagc gcagccgcga gggcgcgcag
      241 atcggccggg tccacgatca cgtcggcgca ggtcagcagg acgtcctcgg cgtcgccggg
      301 aacgccgagc aggccgaggc ggcgggcgtt ggccggcccg ccggcgttgc cgagcagctc
      361 cggctcgacc accacctcga cgccgggccg cccggcgaga tgggcggcca gctggccggc
      421 gtggtggtag gcgttcacca ccacccgctg cgccccggct gccagcagcg ccgcgcaggc
      481 gatgtcgatc agcggccggc cctccaccat caagagcggc ttggcggtcc tgtcggtgag
      541 cggcgcgagc cgggtcccct cccccgccgc caggcagaac ccgatcacgg cgaggacgag
      601 gcggagggcg cgggcgccgc gatgaaccag tcgtccggca cgctgtagcg gggcccctcc
      661 ggggtctcct ccaccagcac gtcggccggc agcgccgcca gccccatcag cccgaggagg
      721 ccccggtgtg cgtgctccga gaggatcacg gtccccgccg ctcccccagc cccgcacgtc
      781 tgcccctccc gcagcccgat cgcccgccgg agctgctcga cccgtcccgc cccgaccgat
      841 tcccgcagct cggcgagatc caccgccggg agcgtcagcc attcgggccg gcgcccgaag
      901 gcgtcctcgt atccctcgct ggccagtcgc agcagcacgg tgatcctgac cgtcggcgac
      961 accggcggat cattgggcag ttccataggc cgttccatcg gccgatggta ggccaagctt
     1021 caggaacccg acagaaatcg atcgagttcg cgtggcggct cggacagagc acgaacgcgg
     1081 ctccgacaga gcacgaacgt gggctccgcc gcgtcctcgg ccgctgaaga actcgcctca
     1141 gacctcggcg gtgccgatca gcccgttgcg ggtgaccaga cgggcgaggt cgggttctgt
     1201 caggttctcg gtggcggggg ggcggcgtgg gaggaccacg tagcgggcct cggcgctgga
     1261 gtcccagacg gtgattccca tctcgggtgg gagtcgcagg ccgaactcgg cgaggacctc
     1321 gcggggttcg cggacgacgc gggcgcggta ggcctcgctc ttgtaccagc ttggggaggg
     1381 gccgaggagg cgcagcgggt agcaggagca cagggtgcag accaccacgt tgtgctgggt
     1441 cgcggtgttg gcgaccaccc gcaggaactc gccgccgcgc ccgccgaggt ccagttcggc
     1501 cagggctgcg ttggcgtcgg cgagcaccag ctcgcggaag tcgcggtcca cccaggcgcg
     1561 ggcggccagc cgggcgccgt tggtgggcga ggcggtggcg aggaaccgct ccagggcctg
     1621 gtcgatctgg gcgtcggtga ggaccccggc ctggatcagg cgttcttcca ggtggcgcac
     1681 ccgggcggcg attggctggc tggcgtgctg gccgctcatg ctccctccgc ttccaccgct
     1741 acgtccacgg cttccaggta ggactcccac aggtcgacgg tcaccgtgtg gctgccctgc
     1801 ccccacaggt cggcggcggc gaaccggacg gtgtagaccg gctccacccg aggcggctcg
     1861 atccggcggg cattgtcgtc gggcagcgga gcggcggcct ggacccggtc gaccacgccc
     1921 acccgcccac gggcgtagcg cggcagccgg gtgtgatgtg gagggtcgac cacggcggtc
     1981 cgcacccgct gcccgggacc gaaccgaggg ccgggacgag ggggcacggc ggcctcacgc
     2041 atccggcagg atgccccgct cacgcagcag cgtctccagc gcgaccagcc accgctcgta
     2101 gtagccggcc cgcaggtact cccgcaccgg catccgctcg atcgcgtcgc ggaactcgtc
     2161 ggtggtgaac aggccctgcc tgccgagcac ggcctggagc gcgaacaccc gcgcctccca
     2221 gtcggcatgg aagggctcaa tatcttcttc gtccccgatt gggacgaggc ccatcccctg
     2281 caggccgccc aggtcgttga tgcgcgccat atcgcgacat gctacggcag tgggagtcgg
     2341 cgtgtaagcc ggcttctgta gccaccgggt tggccggtgg tggcggtcat ccatctgggg
     2401 ccggcgttgc cgccggtctc gtgcggtcca ccagggggct cggccgggcc agcctccgcg
     2461 ccgttcacgg cgagccgtgg gcggcggtac gtcccctact tgaccttgct ccgggtgggg
     2521 tttgccgagc cgaccggtca cccggccgct ggtgcgctct taccgcaccg ttgcaccctt
     2581 gccacacacg aggtgtgtgg cggtctgttc tctgtggcac tttcctgcgg gtcgccccga
     2641 ctggccgtta accagcaccc tgccctgtgg aggccggact ttcctcgatc ccgccggtga
     2701 accggcggag ccgcgaccgc ccggccgact cccactgccg aaccaacagt atgccatccc
     2761 ggtttcgtgc cgagcgggcg catactggaa ccatggttcc actcctcgca gcgctgttca
     2821 tcctggtgcc gctcgccgag ctggccgtga tcatcacggt cgcgcacagc ttcggcgtgc
     2881 tgaacaccct ggcgctgctg ctgctgttca gcatcttcgg cgcctggctc gccaaacggc
     2941 agggctggat ggcctggcgc cgtctccagc tcgccctcga cgaggggcgc ctgccaaccg
     3001 tcgagatcgt cgacggcgcc ctcatcctgc tggccggcgc gctgctgttc acgccagggt
     3061 tcatcaccga cacgatcggg ctgctgctgc tcttcccgcc gacccgggcg gcgctgcggc
     3121 gagtggcgcc caccctggcg ctgcggcgga tgagccgccg cagccgcatc agggtccgcg
     3181 acgagtacgg caccgaggtt cactggggcc ggccgcagat cgacgagcgg gacgagcgac
     3241 gctgacgcct cccccgcgcc ggacgcgccg gcgctagcgc ccggacgggc ggcggacctt
     3301 tccctgggcg tccatggcct ggttggcgag ggcgtcggcc gcgcagttgc gttcgcgggg
     3361 gacgtgctca aaggtgaccc ggtcgaagcc gcggaccagg cggcggacct ccgcccagag
     3421 aggctggagg tcctcgttgc ggaccttcca gatgccgcgt tgctggttga cgaccagcag
     3481 cgagtcggcg cggaccttga cccggcgggc gccgaggacg cgggcgtgct ccaagccggc
     3541 gatcacgccc ttgtactcgg cgacgttgtt ggtggcccag ccgatgccct gcgagagctt
     3601 ggcgaggatg cggccgtcgg gggtctcgac cacggcgccg tagcccgcgg ggccggggtt
     3661 gccgcgggcg ccgccgtcgg cccagatcac cacgtcctcg acccgctcct gctccaagtc
     3721 cagctccagc gtcggctcgg cctccggttc ggttatcacg atttgcgcga agctccttat
     3781 tcgatcacca ggatgcggcg gcaattctcg caccgcttga ctcgttcggg tgggagcttg
     3841 cgcagggcgg ccagctcgac gtgggagacc gacacgcggc agccctggca ggtgttgccg
     3901 accagggcac cggcgccgat gccgccgtag cgggcacgga tctgctcgta gaggcgcagc
     3961 agctcctcgc cgatgccggg ggccagcgcg gcgcgggcgg cgcgctcgac ctccagctcg
     4021 cgctcgatct cagcgagggc ggcgtcgcgg gcctcggtga ccttggcctg ctcggcggtg
     4081 acggcctcac ggcggccggc cagctcggcc agctcggcct ccagagtctc gcgctgctcc
     4141 atcttttcca gcagctcgtc ttcgagggtg gcctgacggc ggtggaggga ggcgacctcc
     4201 tcctgaatgg cggtcagctc cttggggctg gtgaccttgc cggagaccgc gcgggcgtcc
     4261 tcgctggcgg ccttgcgggt gaccatctcg acgtcggttt ccaggcgggc ctgctcgcgg
     4321 ctgagcgtcg cgagcgtccc ctgccgttcg gcggtgagct ggtcgatggt ggcgagcgcc
     4381 tgggtcagct cttccagctt ggcgtgctcc ggcaggttct ggcggcggtg cgccagccgg
     4441 tccatggccg agtcgtggcc ctgcagctcg accagggcgc gctgctgttc tagcggggcg
     4501 tccaacggtg ctcctcgcaa cacaaagggt tcggcggcag cgttacccta ccccgtctgt
     4561 ggcctcgtcc gctttgacgc tatggaggcg gcggtcgtcg gaggtcaggc agcgcccggt
     4621 ggcgaggtcg aactgccagc cgtgcatgcg gcaggtgagc acgccgtcct cgaccacgcc
     4681 gaactgggcc aggtcagcct tgaggtgcgg gcagcggcgc tgcaccaggt agccgtccat
     4741 cgggaccagc tcctcggccg cgcccagctc ggcgtaccag ccctccgcgt actgcagccg
     4801 ctccggcgac agcaccttga accagttgta gacgtactcg ttgaaggggc cgtcgcggga
     4861 cgcctcgaaa cggcacgaca ggaacagctc gttgacccag tcctcgtcgt ggcgggcgat
     4921 gcacgcctcc accagggcgc ggtcgatgcg gaaggtgtag cggcacggct cgcccttcca
     4981 gaggtccacg cggcgatcga ggaagtcgat caccacctgc tcgccctcca ccggatcggt
     5041 ggcgtccaag agcacccggc cgttcacgcc cgcgcaggtc cggtcggcct gcgccagcag
     5101 cggttcccac caggccttca gggagctgac gatgtcgatc tggtggcgtg gccagctcgc
     5161 cttgcggcgc gcgatctcgg gcagctggcg ctgctggtag gcacgcagat aggacagctt
     5221 gtcgctgaag atcgcgtcga cggcgtcgtc gccggggtgc tcgacccggc acgaacccgg
     5281 ggtgacggcg gcgaccgacc cggggatcag caggcggccg ttgtccatgc cggagtcggc
     5341 caaaagctcc aggaacaccg tctggtcggg gaaggtgttg gccgggtcgc ggtcgatgtc
     5401 gttgaagccg aacaggtccg ggtccaggaa gcagggcggc ccggcgctcg ggaagacgaa
     5461 gctggcgccc agttcctcgg cgtagcgctt ggcgcgggcc atctcgttct ggcgcttgcg
     5521 gtggccgagg ttctgcttca tgcgctcggg gaagtcgtac accatcgggt accagatcgc
     5581 cccggagaac tgcaggaagt gggcgtcgaa atgcccgaac gcgcgcagcg gctccaggtc
     5641 gatcgggcgg gagtcgttct ggttgaacag cttggtggtc ccgtcgtcga cggccaggcc
     5701 ggagtcgccg atggggccgt cggtcggggc gaccagcgcc atgatcatga tccgcaggcc
     5761 gtccagctcg accggttcgc tgttgcgggt cttgacgaac cgggtgaagc cgagcgcgcg
     5821 cagctcctgc tccaggtggt tcatggggta gtcgggcagc agcacgatgg cgcgcttgtc
     5881 gacctgctcg cccaggaagt cggggtcgaa gtggtcgtgg tggaggtggg agacgtagag
     5941 gtagtcgggg tggctgatcg cgtcgaggtc cacgtcctcg ttgctcggga acgggaacca
     6001 ggaggcgaag aaggccgtgt tgaaccaggg gtcgcagagg atgctcccgt ggtcggtttc
     6061 gacgtacagc ccagcctgac cgaggaaggt tacctgcatc gtgttcgcaa tcctgaggtt
     6121 ggaggagggc gcctggcggc gcgtggggca cccggacagt aaaccatcag tgtggcggct
     6181 acccggcgaa gcaggcgctc agtaggccct cgccaccagc acggcggcgg tcgccggggc
     6241 gccgtcgacc aggcaggccg cggccggcgg gtcgtcgggc acgatcgcgc ggatactggc
     6301 gccggtgccc tcgccgaggg cgtcctcgca ttcgggcttg ccgcaccagg cggcgcggaa
     6361 gaagccgccg ccaccgatcc gctggcggaa cccgtccagg tcggtgacgg tgtgggtgtt
     6421 ggcctcccgg aaggcgagcg agtcggcgtg cagcgcctgc tgctgctggt cgagcaggcc
     6481 ccgggcggcg cgggcggcgc cgtcaagcgg caccggggac ttcgcgcggg tgtcgcggcg
     6541 gaccagggtg gcctggccgg cggccagctc gcgggcgccg agctcga
//
LOCUS       DASWDC010000216         3459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1224417, whole genome shotgun sequence.
ACCESSION   DASWDC010000216 DASWDC010000000
VERSION     DASWDC010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224417"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2475
                     /locus_tag="VF995_07020"
     CDS             <1..2475
                     /locus_tag="VF995_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX9343349.1"
                     /translation="SAMHTDVPGATVGLIDALAAVGIRYLSAAHNWAGRSVPYLVGGQ
                     ALTRPFWWRAPSGNRLLVWHTDTPHGIAYMEGNLAGLAAGYDLALDLIPAYLSALASR
                     PWPYGGRAFGWSGLPEGAEVTMRPYPHDIVHLRVQGSDADNAGPSVIPASIVREWNQT
                     WAFPRLRMATNHDFLAAAEERLGDALDEFEGDWTDWWADGLGSGARPLGYARRAQRNL
                     QTAETLHALADVRPPAEEGDRVVGTTATTTVDATYDKLALFDEHSWGSANPWTDGEEG
                     VDSGAIQWARKASFAHDGADDALDLLHAGIRRFGATFARSDGALASVVVFNPSSASGP
                     RTDLAEVWVPASVVDPAVPLALLDGRDGTPVPLLAGPPETVPFRPRGRRLRFVAKDLP
                     PLGYARLDLVTGPAEAASGTERAAVSAGLAAAGPGSAGAAAIAASNGVVAIENEFYRV
                     GYDLTEGFIASVFDKVAGRELVNQEAVGGLNQYVRDRYATAPHFNHLSGHVIATDLTL
                     LGGRFTGHGATVVRAERTPVGERLEVELHGDGTDWIRTVVELPRGVRRVDLTNRIAKI
                     GGAAKESVFFAFPFALRSPPAAWELAGGIGGSDLPRVPGSAHYLQYLRHWVAFEEPGL
                     SLAWATLEAPLVQFGNIHLPYAPFPPSLDLPHPEPATIWSWAMNNIWDTNFPSEQRGE
                     TTFRYALTSVEAIPARELGPAAAAGLVEPPVAVLIGGPAPAAGAGARPAAPAAGTFVT
                     VSDPLVEVTSLGRSRHGHALVVRLRSLAPETVEATLTFPLLQPSRAFAGTLLEEGLAE
                     VAVEAEDGAVRVRLPACGTAAVTLDL"
     gene            2527..>3459
                     /locus_tag="VF995_07025"
     CDS             2527..>3459
                     /locus_tag="VF995_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018584014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX9343350.1"
                     /translation="MRIVDIRPTTVAVPLEAPLRHSNGAHWGRFVRTIVEVEADNGLV
                     GLGEMGGGGESAEAAFRHLKDYLVGHDPIQLEALRWKIANPTASLYNNRVQLLAAIEF
                     ACVDLAGQHLGVPACDLLGGRLRDEVPFASYLFFRYPDPASGAGEVRTLDQLVEHARK
                     LKAEHGFTVHKLKGGVFPPGYELACYRALAEALPGDRLRYDPNAVPSVEEAIRFGRAI
                     EDLDNDYLEDPTWGLAGFRRVRERVRVPLATNTVVVDFEQLATNVLQPAADVVLLDTT
                     FWGGMRACVKAAAVCETFQLGIAVHSSGELGVQLA"
BASE COUNT          462 a         1327 c         1197 g          473 t
ORIGIN      
        1 agcgcgatgc acaccgacgt ccccggcgcc accgtcgggc tgatcgacgc gctggccgcc
       61 gtcgggatcc gctacctgtc ggcggcgcac aactgggcgg gccgctccgt gccctacctg
      121 gtcggcgggc aggcgctcac ccggccgttc tggtggcggg cgccgagtgg caaccgcctg
      181 ctggtgtggc acaccgacac tccccacggc atcgcctaca tggagggcaa cctggccggc
      241 ctggccgccg ggtacgacct cgccctggac ctgatcccgg cctacctgtc cgccctcgcc
      301 agccggccgt ggccctacgg cggcagggcg ttcggctggt cagggctgcc ggaaggggcc
      361 gaggtgacca tgcggcccta cccgcacgac atcgtccacc tgcgggttca gggcagcgac
      421 gccgacaacg ccggaccgag cgtgatcccg gcgagcatcg tgcgcgagtg gaaccagacc
      481 tgggcgttcc cgcgcctgcg catggccacc aaccacgact tcctcgccgc cgccgaggag
      541 cgcctcggcg acgccctcga cgagttcgag ggcgactgga ccgattggtg ggccgacggc
      601 ctcggctccg gcgcccgccc gctcggctac gcccggcggg cacagcgaaa tctccaaacg
      661 gccgagaccc tgcacgcgct cgcggacgtc cgccctccag ctgaagaggg ggaccgggtc
      721 gttggcacga cggcgaccac cacggtagac gccacctacg acaagctcgc cctcttcgac
      781 gagcattcct ggggatccgc caatccgtgg accgacgggg aggagggggt ggactccggc
      841 gcgatccagt gggcgcgcaa ggcgagcttc gcccacgacg gcgccgacga cgccctcgac
      901 ctgctccacg cgggcatccg gcgcttcggt gcgaccttcg cgcggtcgga cggggcgctc
      961 gcctcggtcg tggtcttcaa cccttccagc gccagcgggc cccgcaccga cctggccgag
     1021 gtctgggtgc cggccagcgt ggtcgacccc gcggtgccgc tggcgctgct cgacggccgt
     1081 gacggcaccc cggtgccgct gctggccggg ccgccggaga cggtcccctt ccggccgcgc
     1141 gggcgccgcc tccgcttcgt cgccaaggat ctgccgccgc tcggctacgc ccgcctcgac
     1201 ctggtcaccg gcccggccga ggcggcgtcg ggaaccgagc gggccgcggt gtccgcgggc
     1261 ctggccgcgg cggggccggg ctccgcgggc gccgcagcga tcgccgccag caacggcgtg
     1321 gtcgcgatcg agaacgagtt ctaccgggtc ggctacgacc tcaccgaggg cttcatcgcc
     1381 tccgtgttcg acaaggtggc gggccgggag ctggtcaacc aggaggccgt cggtggcctc
     1441 aaccagtatg tccgcgaccg ctacgccacc gcgccgcatt tcaaccacct ctccgggcac
     1501 gtcatcgcca ccgacctgac cctgctcggc gggcgcttca ccggccacgg cgccaccgtg
     1561 gtgcgcgccg agcggacccc ggtgggggag cggctggagg ttgagttgca cggcgacggc
     1621 accgactgga tccgcaccgt ggtcgaactg ccacgcggcg tgcggcgggt tgacctgacc
     1681 aaccggatcg ctaaaatcgg cggcgccgcc aaggagagcg tcttcttcgc cttcccgttc
     1741 gccctgcgga gcccccctgc cgcctgggag ctggccggcg ggatcggcgg cagcgacctg
     1801 ccgcgggtgc ccggatcagc ccactacctg cagtacctgc ggcactgggt cgccttcgag
     1861 gagccggggt tgtcgctggc ctgggcgacg ctggaggcgc cgctggtcca gttcggcaac
     1921 atccacctgc cctacgcgcc cttcccgccg agcctcgacc tgccgcaccc cgagccagcc
     1981 accatctggt cgtgggcgat gaacaacatc tgggacacca acttcccgtc cgagcagcgc
     2041 ggcgagacca ccttccgcta cgcgctcacc tccgtcgagg ccatccccgc ccgcgagctt
     2101 ggtcctgccg ccgccgccgg gctggtcgaa ccgccggtcg ccgtcctgat cggcggccca
     2161 gccccggccg cgggcgccgg cgcccgtccc gccgcacccg cggccggaac tttcgtcacc
     2221 gtcagcgacc cgttggtcga ggtcacctcc cttggccgct ctcggcacgg ccacgccctg
     2281 gtggtgcggc tgcgctcgct ggctccggag acggtcgagg cgaccttgac gttcccgctg
     2341 ctgcagccat cgcgcgcctt cgcagggacg ctcctggagg agggcctcgc cgaagtagcc
     2401 gtggaggctg aggacggcgc cgtgcgggtg cggctgcctg cctgcgggac ggcggcggtg
     2461 accctggacc tctgagttcc aagtaggaag cgaagcgagc acgaagtacg cgaggagagc
     2521 acctccatga ggatcgtcga catccggccc accacggtgg ccgtgccgct cgaggcgccc
     2581 ctgcggcact cgaacggcgc ccactggggc cgcttcgtcc gcacgatcgt cgaggtcgag
     2641 gcggacaacg gcctggtcgg cctcggcgag atgggcggcg ggggagagag cgccgaagcc
     2701 gccttccgcc acctcaagga ctacctggtc ggccacgacc cgatccagct ggaggcgctc
     2761 cgctggaaga tcgccaatcc cacggcgagc ctctacaaca accgcgtcca gctgctcgcc
     2821 gccatcgagt tcgcctgcgt cgacctggcc ggccagcacc tcggcgtccc tgcctgcgac
     2881 ctgctggggg gcaggctccg cgacgaggtc ccattcgcca gctacctctt cttccgctat
     2941 cccgacccgg cgagcggcgc cggcgaggtc cgcaccctcg accagctggt cgagcatgcc
     3001 cgcaagctca aggccgagca cggcttcacc gtccacaagc tgaagggtgg ggtgttcccg
     3061 cccggctacg agctggcctg ctaccgcgcg ctggccgagg cgctgcccgg cgaccggctg
     3121 cgctacgacc ccaacgccgt cccgagcgtc gaggaggcga tccgcttcgg ccgggccatc
     3181 gaggacctgg acaacgacta cctggaggac cccacctggg gcctcgcggg gttccgccgg
     3241 gtccgcgagc gggtgcgggt cccgctggcc accaacaccg tcgtggtcga cttcgagcag
     3301 ctcgcgacca atgtgctcca gcccgccgcc gacgtggtcc tgctggacac caccttctgg
     3361 ggcggcatgc gcgcctgcgt caaggcggcg gcggtctgcg agaccttcca gctcggcatc
     3421 gccgtccact cctccggcga gctgggcgtg cagctcgcc
//
LOCUS       DASWDC010000217        22568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_446296, whole genome shotgun sequence.
ACCESSION   DASWDC010000217 DASWDC010000000
VERSION     DASWDC010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22568
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446296"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VF995_07030"
     CDS             <1..260
                     /locus_tag="VF995_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX9343351.1"
                     /translation="EPLGLAPHSPAVLVVVGMAACLGTIAHVPIAVTVMAVEVTGSMD
                     VLLPAMIAVSTAAFVVGDATLYRKQLATRADQAARSGVEIN"
     gene            complement(257..1381)
                     /gene="dnaJ"
                     /locus_tag="VF995_07035"
     CDS             complement(257..1381)
                     /gene="dnaJ"
                     /locus_tag="VF995_07035"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX9343352.1"
                     /translation="MTDYYDLLGVSRDASQEEIKRAYRRLARQHHPDVNSGDAAAEER
                     FKEVSKAYSVLSDPDKRQRYDAFGEEGVPAGAGFADDPFGFATFEDLVGQFFGTGPFA
                     ARSRPRSRARRGADTAARVELRFEESVFGAKKEVPVRLAGACDRCHGSGAERGTDPTV
                     CPRCNGSGQQRTVRQSVLGQIVTASTCAKCSGSGYEVRTPCTRCHGQGVVAKDVKIAI
                     DIAPGVEDGTRERLPGRGHAGQYGGQPGDLWIEVTVAPHPVFGRNGHDLTCKLSVPLT
                     VAALGGTLSLDTLDGDELPVEVEPGTQSGTIKRFRKRGVPASDGWGRRGDLLVELAVE
                     TPTDLADEERRLLRSLAELRGETGHLSSSGLFSRLRGSAR"
     gene            complement(1420..2454)
                     /gene="hrcA"
                     /locus_tag="VF995_07040"
     CDS             complement(1420..2454)
                     /gene="hrcA"
                     /locus_tag="VF995_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX9343353.1"
                     /translation="MSLDERKALLLRAVVHDFIQTAEPVGSKALSERYTLGVSPATIR
                     NELAVLEAEGYLSHPHTSAGRVPTERGYRFYVDSLSGVNDLAHAQEEAVVRYLTGTAG
                     LEEILQRTSLLLATLTRYTAMVTTPAIDNARLRHVELVLLSPHVVLMVLIVDTGQVSK
                     RIIESASEVTNEDLDELRRQLNDKLVRERLSRAELALSGMAPQVRPERRGLFAVLADA
                     IGQLASDRSTERVWVGGQAHIAGPGVFDGLDTIRRVYEALEQQVLLLRLLEATMRTSD
                     HQLSVVIGSENKVEGMEAVSLVTATYPAGDTTGTIGVLGPTRMDYLRAMAAVQAVASY
                     LGAVLDDVER"
     gene            complement(2559..3809)
                     /gene="hemW"
                     /locus_tag="VF995_07045"
     CDS             complement(2559..3809)
                     /gene="hemW"
                     /locus_tag="VF995_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX9343354.1"
                     /translation="MTVATLADPAARREVDAVLTDPALFGVYAHVPFCLSRCHYCDFV
                     TYTGMEGLRRPYAAALLAEAELAVAALVRAEAERPTVTSVFVGGGTPTLLPAADLARV
                     LDRLRALLPFAPDPEVTVEANPETVTPEVAATLVAAGVNRVSMGAQSFDGQVLAALGR
                     THDARRPGEAVAALRAAAARAGADLSLSLDLIYGCPEEDEASWVSSLAAAVALEPDHL
                     SAYALTIEPATKFGRLVAAGRMAEPDEDLLADRYHTTCEILAAAGYEHYEVSNWAKPG
                     QASRHNRTYWRRGHYLGLGAGAHEFTGETRRWNTGSVPDYLAAVRAGRRPVAGEETLD
                     AGQARFEALALRLRTSDGLSAAEARVLGADPTGAEVANLRAAGLLAHDNPGDQDQGQG
                     PGPGLRLSEQGMFLHGEVVSRLAP"
     gene            complement(3806..5623)
                     /gene="lepA"
                     /locus_tag="VF995_07050"
     CDS             complement(3806..5623)
                     /gene="lepA"
                     /locus_tag="VF995_07050"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008381065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX9343355.1"
                     /translation="MDLARLRNFCIIAHIDHGKSTLADRLLDLTHAVNERQMRAQYLD
                     KMDLERERGITIKAQAVRLAYQAADGLDYVLNLIDTPGHVDFTYEVSRALEACEGAVL
                     LVDAAQGIEAQTLANLYLALEAELAIIPVINKIDLPAAQPERVAREIEQVLGVPAEEM
                     LRVSAKTGEGVPILLEALVRRVPPPKGDPGAPARALIFDSLYDSYRGVIAYVRMIDGR
                     LVSHERVKMMATGVVSEAEEVGVFAPEAVPTKDLAAGEVGYLIPGVKDVRQAKVGDTI
                     TAAGPAGAATPLPGYREPRPMVYSGLYPIDGDDFPDLRDALDRLRLNDAALVYEPETS
                     AALGFGFRCGFLGLLHMDIVRERLEREFDLDLITTAPNVAYHVFTEDGRELTVSNPSE
                     LPEAGRVQRIEEPYVSSMVLAPADFIGPVMELCQSRRGVLDTMEYLSEERVELRYQLP
                     LAEIIFDFFDQLKSRTRGYASLDYEPSGYRAADLVKVDLLLQGEPVDAFSAIVHRDKA
                     YAYGRAMATKLKRLIPRQLYEVPIQATIGSKVIARETITARRKDVLAKCYGGDITRKR
                     KLLERQKEGKKRMKQIGRVEVPQEAFVAALRTDDGDNSR"
     gene            5904..6170
                     /gene="rpsT"
                     /locus_tag="VF995_07055"
     CDS             5904..6170
                     /gene="rpsT"
                     /locus_tag="VF995_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016443759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX9343356.1"
                     /translation="MANIKSQIKRNRQNEARRMRNKSVRSALKTYARRVRESLATGDS
                     ATAETAYRHAARAYDKAASKGVIHPNNAANHKASLAKALHRVSE"
     gene            complement(6260..7216)
                     /gene="holA"
                     /locus_tag="VF995_07060"
     CDS             complement(6260..7216)
                     /gene="holA"
                     /locus_tag="VF995_07060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX9343357.1"
                     /translation="MAPVTLVIGEEELLAERAVAEAVAAAKAGLGAGTSVEEVSGGAL
                     PDGFAMGLATASLFGGGRIVVVREAETLDAAARGAVLAAAADPSPGTVLVLRAAGPGR
                     VAKFFKDLQQHAVTVTAAKLKPSERAAWLRAEIRRLGRKADDGAVAALLDTVGQDLRE
                     LAGAALKLHVAVPPPTVWTRAQVGEFLTPTADRGVFELTDAVLGGEPQAALRHLDSLL
                     GQGEEVLGLLGMLARQLRLLLRVVDYGNAPKGQVAQAIGGGVRDWQVDRARRQARGFR
                     PDDLRRALDLIAAADAEVRNGGLPARLLAELVVTRIAAAGAR"
     gene            complement(7250..8875)
                     /locus_tag="VF995_07065"
     CDS             complement(7250..8875)
                     /locus_tag="VF995_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX9343358.1"
                     /translation="MRPDDQRSGQRWRRGRDPDPDGVALAATVAAGVLLGGCAAVAAG
                     THATPLAVLLALAAAGLAAMPLSEAAERPVLAGAMVLVTLATTGAAVAAMRVAGTSGG
                     LLPRLAATPGRGVAVTGTVVEEPRTVQYGARLVVLRVDRVTTAGRTWRTRERAAVVFG
                     RDAAPSVSTVAVGDQLRLHAGVAPAAHPSRIGVSDQAPVTLRRPHLEAWAPSRSRLLR
                     SSEAFRAAARGSARASLPPEHAGLLVGMALGDTSLLSPTLDRVFKDAGLSHLLAASGE
                     NLAVLLAAGLGLAAALGAGRPSLAAVGVLLTIAFALLTRWEPSVLRASVMAALVLAGV
                     ATGRGPGGRRALCLAVMFLLLANPSLAATLGFQLSVAATAGVLGLGPANRPRAAPVAA
                     AAGPRRRRLRTRRPGRRAPRRRAGTRAALPRRPAREPPRAPAGDSADAPRCGCRARRA
                     GRPAARRRGLPPGRPIPRGPAGRRPGRRPAPGRDRHPARPRPSAASHHCPGVRSDRQA
                     MASPVWPGAGGQVTVAAIDFPASVTGPSVTAAG"
     gene            complement(8865..9647)
                     /locus_tag="VF995_07070"
     CDS             complement(8865..9647)
                     /locus_tag="VF995_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEX9343359.1"
                     /translation="MPTPPSFVLPPDRLTARLRDHAGRLLAGVGRQRVVLSVVALVAL
                     ACAAVIWFRGTPRPIDPTMAGARASASGAMLTSVTGADATPPTPPGSGMGANAVGAGT
                     GADTGTGALAVDVVGRVRHPGLVHLPVGSRVIDAIQAAGGAAPGADLDAVNLARKLAD
                     GEQLRVPRHGEAAVLPTTAQLDPAGSTGLGGPPSGPLDLNTTTTEQLDTLPGVGQVTA
                     SRIIAYRAAHPFTSVDDLRQVPGIGDRRFATLKDLVTVSNAP"
     gene            complement(9883..12348)
                     /gene="leuS"
                     /locus_tag="VF995_07075"
     CDS             complement(9883..12348)
                     /gene="leuS"
                     /locus_tag="VF995_07075"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX9343360.1"
                     /translation="MDPERYDPQQVEPKWTAAWEERALYRADENDTERARFYALDMFP
                     YPSGDLHMGHLEAFAGGDVIARLKRMQGYNVLHPIGWDAFGLPAENAAISRGIHPKTW
                     TRQNIDTQRATFQRLGISFDWSRTFNTCDPDYYRWTQWIFLRLYERGLAYRKASPVNW
                     CPHDQTVLANEQVINGHCERCGHLVEKRELTQWFFRITEYAQALLDDMAELEDRWSDK
                     VLTMQRNWIGRSVGAYVDFIISETGQTVRIFTTRPDTLYGATFFVLAPEHQLAEQLVA
                     GTEHEAAFRRFRDTVDRETEVERLSTERDKTGVFLGAYAINPVNGERIPVWAADYVLL
                     EYGTGAIMAVPAHDQRDFEFARNYGLPVRIVVQPEGEPPLDPGQMTEAMGHEGVMVNS
                     GPYDGIPAGEAIRRVTADLAAQGRGEFAINYRLRDWLVSRQRYWGCPIPIIHCPSCGE
                     VPVPDDALPVPLPDDVADFRPKGESPLATVSDWVNVACPRCGTPARRETDTMDTFVDS
                     SWYFLRYTGLDPERPFDPDRVARWMPVHQYTGGIEHAILHLLYSRFFVKFLHDLGLVG
                     FREPFRALLNQGMVVMHGAAMSKSRGNLVEPATIIDEHGADVARLTMLFAGPFEDDVD
                     WADVSPEGMARWIHRVWRAVRGAADSGAGHGGASDPALVRHAHRATQAVSDDLERFRF
                     NTAVAKLMTLSNDLADAVRSGRGSATEQRDTAERLVLMLAPLAPFVTEELWHALGHTE
                     SVHLAAWPGYDPELVRVERLTCVVQVDGKVRDRIEVTADAGEDDLRALALASPKVKAA
                     LADRPIARVITVPPKLVNLVTAR"
     gene            12786..12974
                     /locus_tag="VF995_07080"
     CDS             12786..12974
                     /locus_tag="VF995_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9343361.1"
                     /translation="MLLRTTEVARRLQVSARTVLAWAQAGKLPSILTPGGHRRYPAQG
                     VEQVLATMTAAGEPRVDE"
     gene            13178..14224
                     /locus_tag="VF995_07085"
     CDS             13178..14224
                     /locus_tag="VF995_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343362.1"
                     /translation="MEPATEGRGTPLSLPRTAAWVWHAEPRSIFGLDAVFVRCANGTS
                     TTSTSGDGFDFADNYRRWQDTRGPAVVPWTWFGPPASSDGTAAADLLCQIGGAQPLYV
                     VEIGFKAPAEEVVAFAKRMRERQPAALLGFSTYPTRAEATANGVPWDVCVAVFDLGLP
                     QVYTAKQRLELLRPDSPVVADMAGKPIHVVVCPDSDPGWLEAAKAAMARHAGVSAWAV
                     DQASFTTWRRHLSGLADERPAAKPPSTSRSALTDELPATEAAEEAAPPSPSPLSPGPG
                     PVPAPTPIAAGDGGPAPIPTPPRPAGLEPGAQALLANRIIAIVQAHLDAGGTLSDDQL
                     LSEVERALGDATAG"
     gene            14357..16240
                     /locus_tag="VF995_07090"
     CDS             14357..16240
                     /locus_tag="VF995_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HEX9343363.1"
                     /translation="MQASAATERAAIDAEVSGKTLCDLLLRNAETYGDQPALSWNADG
                     DGDGGWQHLTWRGYRDRVAEAAMGLAALGVGRGDFVAIMTRNRPEHLIADLAALHLGA
                     VPVSFYNTLAPEQIAYIAAHCEAKVAVLDGRQFMERWEKVKDDLPNLTHAVLLEDAAD
                     FADYGWVRSWDALLEGGRAALASADGRARFDAAWRQVTPDDLVTVMYTSGTTGPPKGV
                     MTTHYNALWTAAAVERWTRESNRAAWEPGATYLSYLPLAHSLERLSGHYAAQWRATSV
                     WFCPEVLRVVEYLPQVRPATFVAVPRLWEKAQAGITAALAAEPNPRRRRIAEQAIASG
                     RQAVRLFQAGKPMPLGLRLRRALFERLVFSKIRHRMGLDRCLVAFSGAAPLAEDVLEF
                     FLAIGIPLREGYGLTESTAPASLNLPERVKVGTVGPALPGVEMRVADDGELLIRGGNV
                     AKGYYKDPEQTAATFGPDGWLHTGDVAEVDEDGFIRIVDRKKELLITAGGDNVSPSNL
                     ENLLKAHPLIAQACAVGDRKPYIAALVVLDGEVAPGWAGQHGIPFESLERFALDERIR
                     AELQQAVDQVNQHVARIEQIKRFTVLPTEWTMDSEELTPTLKLKRRVIHQKYAAEIQA
                     LYQ"
     gene            complement(16320..19301)
                     /locus_tag="VF995_07095"
     CDS             complement(16320..19301)
                     /locus_tag="VF995_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9343364.1"
                     /translation="MYPNLTLPLITLMAAALLATALVALRRPFLRRLALRQASRRRGE
                     ALLVIAGSALGTAIIVGSLIVGDTLGFSVKRDADRQLGPIDEIVSAPTPAQGEAAAKR
                     VAVLRGDPDVDGVLTLRIDQAAVAKGDGAARKAEPNANVAEADFGTAQPVAGSDGGAG
                     LDAPTPNPGEVVLNSELAGALGASAGDTLTFWLYGHPTALRVTQVVGSTGIAGAGGNG
                     GRNAFLAPGTLAALAPKDAQPEVVTFVSNTGDIEGGSAHSDAVAGKLTRALAPLAAKA
                     NAKATGTGTGEDAVAVHTVKQHALADAKSAGDQFGSFFLFIGSFSIIAGVMLLVNVFV
                     MLADERKGELGILRAIGMRRGRLVRAFMIEGALYALIACVIGALAGIGVGRAVGIFAG
                     RIFGSFSSDQGGLAIVFHITPVSVVNGLAFGFLIAFLTATLTAVRVSRLNVIAAIRDL
                     PGDGGRRLKRRWVALSTLVAAGLGVLTVPVVANGRGSLVYLLPALAALALCPLLVRLL
                     PKRLAYSLAALGVLGWTLAANTVRPHLFDDGSTGTYVVLGVALTFSAVFLLSQNQQLV
                     TGPLRPLASRATPWGLAARLAFAYPLARRFRTGAILVMYGLVVFTIVLVAVLTSVEDS
                     TRAHEVASATGGFAIRADLNPSAPVTTPDPAAAFTTGQFARKVAAAVPLSAADGRIQA
                     PGAAGQAQPGGESGQDRVTVVGADPRIATAGLFPLDKRLGQFGDDRAVWRAVLNDPRY
                     VITVNALLGGKGETKPGDSFTLTDPRSGKVARKTVAGVLGNPDAFFGPGNDGPPVIMG
                     VPAARAQFGAWLKLSAVLVKPAPGVSDQALAGELQGAFLAQGLVATRIRHEVDQEFAA
                     NTSFDELMEGFLALGLVIGIGGLGVVMVRAVRERRRSVGVLRALGFQARVIHRAFLSE
                     SAFIAAEGIILGAALAVVTDYLLFRNDPALKAAQVAFSIPWLNIAVLLSLTAAASLLA
                     TAWPARQAARLRPAVALRIAD"
     gene            complement(19305..20042)
                     /locus_tag="VF995_07100"
     CDS             complement(19305..20042)
                     /locus_tag="VF995_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9343365.1"
                     /translation="MGEPLLLAEGVRKVYRTGDGGEIEVEALAGLDLAIDRGELVAVM
                     GPSGSGKTTLLNCLSGLDGIDSGRVLVEGQDLFALSDAKRTAYRARAMGFVFQAYNLI
                     PVFTAAENVELPLLLAGAPPRQARSEAAMMLERVGLGHRLRHRPNELSGGEQQRVTIA
                     RALAARPAIVWADEPTGNLDTQMAAQVLALLQDLNRDEGQTVVLVTHDPAIGAAAGRR
                     VRMRDGRVVADEPRPPAYAGQHQGLEG"
     gene            complement(20205..21782)
                     /locus_tag="VF995_07105"
     CDS             complement(20205..21782)
                     /locus_tag="VF995_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9343366.1"
                     /translation="MTGRPHRWSFHSYVGAVITAGALVVYLLAARGGLADVQRAPAAF
                     WLLAVLFTVGEVRPIFILRRGQVEEFTTSTTFTVALLLGWGLGPAALVVLAGSIIADL
                     LRRKALMRTLFNAAQYVLALAAASAVVELVGSGAEWQLSIRGLVALLLTGMAFFAANR
                     ILVRIVIALRQGAPLAPQILDPIGLAALPEALLVGMAPVAVILARQGAATALLLALPM
                     IGVHLAYKAAADAHANRAVAEAAAEEARKIAAEKARLVEAEQEVVRRLLQSDQFKDNV
                     LSTVSHELRTPLAGMLGAVQTLENREERLSDEQKRELVGMAVRQGERLKELIEHLLLA
                     AQVQASGVPLPNRHPPVDAGELAREAVAAAKARYPRRVMELGVEDDLPVHAAPEAVLQ
                     AVSNLLDNAAKYAPSGAPVWVDAFHDGNLVVIAVEDGGPGVPAAKREWIFGRFGKLDG
                     NVVRHADGVGLGLYIARRLARAEGGDLVMAEPTRTGAGARFELRVPPALKWEARSVAD
                     SVAEGIARETESRTSYS"
     gene            22326..>22568
                     /locus_tag="VF995_07110"
     CDS             22326..>22568
                     /locus_tag="VF995_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343367.1"
                     /translation="MSFYADLHIHSRFSRATSRDLDLEQLAWWGRRKGLSILGTGDFT
                     HPAWHQHLREELVPAEPGLYRLRPDLDAAVTQRLPGS"
BASE COUNT         3305 a         7977 c         8203 g         3083 t
ORIGIN      
        1 tggagccgct cgggctcgcg ccgcactcgc cagcggtcct ggtcgtcgtc ggcatggccg
       61 cctgcctcgg cacgatcgcg cacgtcccca tcgcggtcac cgtgatggcg gtggaggtca
      121 ccggcagcat ggacgtcctg cttccagcga tgatcgcggt ctccaccgcg gccttcgtgg
      181 ttggcgacgc gaccctctac cgcaaacagc tcgccacccg cgcggaccag gcggcacgtt
      241 ccggcgtgga gatcaactaa cgggcggagc cgcgcaggcg ggagaagagg ccggatgagg
      301 agaggtggcc ggtctcgccg cgcagctcgg cgagggagcg gagcaggcgg cgttcctcgt
      361 cggcgaggtc ggtgggagtt tcgacggcga gctcgaccag gaggtcgccg cggcggcccc
      421 agccgtcgga ggcggggacg ccgcgtttgc ggaagcgttt gatggtgccg gactgggtgc
      481 cgggctcgac ctcgacgggc agctcgtcgc cgtcgagggt gtccagcgac agcgtgccgc
      541 cgagcgcggc gaccgtcagg gggaccgaga gcttacaggt cagatcgtgg ccattgcggc
      601 cgaagacggg gtggggcgcg accgtgacct cgatccagag gtcgccgggc tggccgccgt
      661 actggccggc gtggccgcgg ccggggaggc gctcgcgggt cccgtcctcg acgccggggg
      721 cgatgtcgat ggcgatcttg acgtccttgg cgacgacgcc ctggccgtgg cagcgggtgc
      781 atggggtgcg gacctcgtag ccgctgccgc tgcacttggc gcaggtcgag gcggtgacga
      841 tctggccgag gaccgactgg cgcacggtgc gctgctggcc gctgccgttg cagcgggggc
      901 agaccgtcgg gtcggtgccg cgttcggcgc cggagccgtg gcagcggtcg caggcaccgg
      961 cgaggcggac cgggacctcc ttcttggcgc cgaagaccga ctcctcgaag cgcagctcca
     1021 cgcgggccgc ggtgtcggcg ccgcggcggg cgcgggagcg tggccggctc cgcgccgcga
     1081 aggggccggt gccgaagaac tggccgacga ggtcctcgaa ggtggcgaag ccgaacgggt
     1141 cgtccgcgaa cccggcgccg gcggggacgc cctcctcgcc gaaggcgtcg tagcgctgcc
     1201 gcttgtccgg atcggagagg acgctgtagg ccttgctgac ctccttgaag cgctcctcgg
     1261 cggcggcgtc gccggagttc acgtccgggt ggtgctggcg ggccagccgc cggtaggcac
     1321 gcttgatctc ctcctgggac gcgtcccggg agacaccgag gaggtcgtag tagtcggtca
     1381 agcgtctccc ctacatcctg gctcgagtgc taacgctttc tagcgctcga cgtcgtccag
     1441 aacggcgccg aggtaggagg caacggcttg caccgcggcc atggcacgaa ggtaatccat
     1501 ccgggtgggt ccgagcaccc cgatcgttcc ggtcgtatcg cccgcggggt aggtggcggt
     1561 caccagcgag acggcctcca tcccctcgac cttgttctcc gagccgatca ccaccgacag
     1621 ctggtggtcg ctggtgcgca tcgtggcctc aagcagccgc agcagcagga cctgctgctc
     1681 cagcgcctcg tagacgcgcc ggatggtgtc gaggccgtcg aacacccctg gaccggcgat
     1741 gtgcgcctgg ccgccgaccc agacccgctc ggtggatcgg tcgctggcca gctgcccgat
     1801 cgcgtcggcc aggacggcga acagcccgcg ccgctcgggg cggacctgcg gcgccatgcc
     1861 ggacagggcc agctcggcac ggctgagccg ctcgcggacc agcttgtcgt tgagctggcg
     1921 gcgcagctcg tccaagtcct cgttggtgac ctcgctggcg ctctcgatga tgcgcttgga
     1981 gacctggccg gtgtcgacga tcagcaccat caggaccaca tgcgggctga gcagcaccag
     2041 ctcgacgtgg cggagcctgg cgttgtcgat cgccggggtg gtgaccatcg cggtgtagcg
     2101 ggtgagggtg gccagcagca gcgaggtacg ctgcaggatc tcctccagcc cggcggtgcc
     2161 ggtcaggtag cggaccaccg cctcctcctg cgcgtgggcg aggtcgttca cccccgacag
     2221 cgagtccacg tagaagcggt agccccgctc ggtcgggacc cgcccggcgc tggtgtgcgg
     2281 gtgggagagg tagccttcgg cctccagcac ggccagctcg ttgcgaatgg tcgcgggtga
     2341 gacgccgagg gtgtagcgct cggacagagc cttcgacccg accggctcgg cggtctggat
     2401 gaagtcgtgg acaactgcgc gcagcagcag cgccttccgt tcatcaaggc tcatgagctg
     2461 agcgtagcag tccgaaaggg cgactgctaa gccgagcagg cggacacatt ggcgcctggt
     2521 ttgtcatgaa catccccgcc cgagggtcca tgcgaccgtc acggcgcgag gcggctcacc
     2581 acttcgccgt gcaggaacat gccctgctcg ctgaggcgca ggcccggacc cgggccctgg
     2641 ccctggtcct ggtcgccggg gttgtcgtga gcgagcaggc cggcggcgcg caggttggcg
     2701 acctcggcgc cggttgggtc ggcgccgagc acgcgggcct cggcggcgct gaggccgtcg
     2761 ctggtgcgca ggcgcaacgc cagcgcctcg aagcgggcct ggccggcgtc gagcgtctcc
     2821 tcgcccgcga cgggacggcg gccggcgcgg accgccgcga ggtagtccgg cacgctgccg
     2881 gtgttccagc ggcgggtctc gccggtgaac tcgtgggcgc ccgcgccgag gccgaggtag
     2941 tggccgcggc gccagtaggt gcggttgtgg cggctggcct ggccgggctt cgcccagttc
     3001 gacacctcgt aatgctcgta gccggcggct gcgaggatct cgcaggtggt gtggtagcgg
     3061 tcggcgagca ggtcctcgtc cggctcggcc atgcggccgg cggcgaccag acggccgaac
     3121 ttggtcgccg gttcgatcgt gagggcgtag gcggacaggt ggtccggctc cagggcgacc
     3181 gcggcggcca gggagctgac ccaggacgcc tcgtcctcct cggggcagcc gtagatcagg
     3241 tcgaggctga ggctgaggtc ggcaccggcc cgggcggcgg cggcgcggag cgccgcgacc
     3301 gcctcgccag ggcggcgggc gtcgtgggtg cggccgagcg ccgcgagcac ctggccgtcg
     3361 aagctctgcg cgcccatcga gacgcggttc accccggcgg cgacgagggt ggcggcgacc
     3421 tcgggggtga ccgtctccgg gttggcctcg acggtcacct ccgggtcggg cgcgaacggc
     3481 agcagggccc gcaggcggtc gaggacccgg gccaggtccg cggccgggag cagcgtcggc
     3541 gtgccgccgc cgacgaacac gctggtcacg gtcgggcgct cggcctccgc acgaaccagc
     3601 gcggcgacgg ccagctccgc ctcggcgagc agcgccgcgg cgtacgggcg ccgcaggccc
     3661 tccatgcccg tgtaggtgac gaagtcgcag tagtggcagc ggctcaggca gaacgggacg
     3721 tgcgcgtaga ccccgaacag cgccgggtcg gtgaggacag cgtcgacctc ccggcgggcc
     3781 gccggatcgg cgagcgtcgc gaccgtcacc ggctgttgtc cccgtcgtcg gtccgcaggg
     3841 cggcgacgaa ggcctcctgg ggcacttcga cgcggccgat ctgcttcatg cgcttcttgc
     3901 cttccttctg gcgctccagg agcttgcgct tgcgggtgat gtcgccgccg tagcacttgg
     3961 cgagcacgtc cttgcgccgc gcggtgatgg tctcgcgggc gatcaccttc gagccgatcg
     4021 tcgcctggat cggcacctcg tagagctggc ggggaatcag ccgcttcagc ttggtcgcca
     4081 tggcgcggcc gtaggcgtag gccttgtccc tgtggacgat cgcggagaag gcgtccacgg
     4141 gttccccctg cagcagcagg tcgactttga ccaggtcggc ggcgcggtag ccgctcggct
     4201 cgtagtcgag cgaggcgtag ccgcgggtgc gcgacttcag ctggtcgaag aagtcgaaga
     4261 tgatctcggc gaggggcagc tggtagcgca gctcgacccg ttcctccgag aggtactcca
     4321 tggtgtcgag cacgccgcgg cggctctggc acagctccat caccggcccg atgaagtccg
     4381 ccggggccag caccatggag gacacgtacg gttcctcgat ccgctggacg cggcccgctt
     4441 cgggaagctc gctggggttg gagacggtca gctcgcggcc gtcctcggtg aagacgtggt
     4501 aggcgacgtt cggggcggtg gtgatcaggt cgaggtcgaa ctcgcgctcc aggcgttcgc
     4561 gcacgatgtc catgtgcagc aggccgagga agccgcagcg gaacccgaat cccagcgccg
     4621 ccgaggtctc cggctcgtag accaaggccg cgtcgttgag gcgcagccgg tcgagcgcgt
     4681 cgcgcaggtc cgggaagtcg tcgccgtcga tcgggtacag gcccgagtac accatcgggc
     4741 ggggctcccg atagccgggc agcggggtgg ccgccccggc cgggccggcg gccgtgatgg
     4801 tgtcgccgac cttggcctgg cggacgtcct tgaccccggg aatcaggtag ccgacctcgc
     4861 cggccgccag gtccttggtg ggtacggcct cgggggcgaa caccccgacc tcctcggctt
     4921 cggagaccac gccggtggcc atcatcttga cccgctcgtg cgagaccagg cgcccgtcga
     4981 tcatccggac gtaggcgatg acgccccggt aggagtcgta gagcgagtcg aagatcagcg
     5041 cgcgggcggg cgccccgggg tcgcccttgg gcggcgggac gcgccggacc agggcctcca
     5101 gcaggatggg gacgccctcg ccggtcttgg cggagacgcg cagcatctcc tcggcgggga
     5161 cgccgagcac ctgctcgatc tcgcgggcga cccgctcggg ctgcgccgcc gggaggtcga
     5221 tcttgttgat caccgggatg atcgccagtt cggcctcgag cgccaggtag aggttggcga
     5281 gcgtctgcgc ctcgatgccc tgggcggcgt cgaccagcag gaccgcgccc tcgcaggcct
     5341 caagggcccg cgagacctca taggtgaagt ccacgtggcc gggggtgtcg atcaggttca
     5401 gcacgtagtc gagcccgtcg gcggcctggt aggcgagccg gaccgcctgc gccttgatgg
     5461 tgatgccgcg ctcgcgctcc aggtccatct tgtcgaggta ctgggcgcgc atctgccgct
     5521 cgttcaccgc gtgggtcagg tcgagcagtc ggtcggcgag ggtcgacttg ccgtgatcga
     5581 tgtgggcgat aatgcagaaa ttgcgtagac gcgcaaggtc cattggcagc ggcgccgaag
     5641 gcggcgatcg gttgtcgccg ggcttcaatg gtcctccgct tcgccgacct ccggcccggc
     5701 aggcggcgac tggtcgacgt ttcgaggcgg ttccatgggt gtgctctagt ccttcgggac
     5761 gatccaactg ggcagcgagc atacctgacg gcgctggtcg cgggccccct cgcaccgccg
     5821 tgtgacgttg agggtacgcg agccgctgtg ctagcctgcc ggagttgcga ccaatcccat
     5881 gcagagcaga gggctgacct tccttggcca acatcaagtc ccagatcaag cggaaccggc
     5941 agaacgaggc gcgccggatg cggaacaagt ccgtccgctc cgcgctcaag acctacgccc
     6001 gccgggtccg cgagagcctc gccaccggcg acagcgccac cgccgagacg gcctaccgcc
     6061 acgcggcccg cgcctacgac aaggcggcca gcaagggggt catccacccc aacaacgccg
     6121 ccaaccacaa ggcgagcctc gccaaggctc tgcatcgcgt cagcgagtag gggccctcgg
     6181 cgtcaccgac attgagggcg gccacctggc cgcccttctt gctgtctcgc cgtatcgggc
     6241 gcccgcctta tcggccgcct tatcgggcgc ccgccgccgc gatccgcgtc accaccaatt
     6301 ccgccaggag ccgagccggc aggccgccgt tgcggacctc cgcgtcggcg gcggcgatca
     6361 agtcgagcgc gcgccgcagg tcgtccgggc ggaacccgcg cgcctggcgc cgggcccggt
     6421 caacctgcca gtcgcggact cccccgccga tcgcctgcgc gacctggccc ttgggggcgt
     6481 tgccgtagtc gaccacccgc agcagcagcc gcagctgccg tgccagcatc cccagcagcc
     6541 ccagcacctc ctctccctgg ccgagcaaag agtccaggtg ccggagggcg gcctgtggct
     6601 ccccaccgag caccgcgtcg gtcagctcga agactccccg atccgctgtg ggggtcagga
     6661 actcccccac ctgcgcccgg gtccacaccg tcggcggcgg caccgccacg tgcagcttca
     6721 gcgccgcgcc ggccagctcc cgcaagtcct gcccaaccgt gtccagcagc gccgcgaccg
     6781 cgccgtcgtc ggccttgcgg ccgaggcggc ggatctcagc ccgcaaccag gccgcccgct
     6841 ccgacggctt cagcttggcg gccgtcaccg tgaccgcgtg ctgctgcagg tccttgaaga
     6901 acttcgccac ccggccaggc ccggctgccc gcagcaccag cacggtcccc gggctcgggt
     6961 ccgccgccgc cgccagcacc gccccgcgcg ccgccgcgtc cagcgtctcc gcctcccgca
     7021 ccaccacgat gcggccgccc ccgaacaacg acgcggtcgc caggcccatc gcgaacccgt
     7081 ccggcagcgc cccgcctgag acctcctcga cgctcgtgcc ggccccaagg cccgccttgg
     7141 ccgcggcgac cgcctcagca accgcccgtt cggcaagcag ctcctcctca ccgatcacca
     7201 gcgtgacggg cgccatccct tgcgtactct gactcggcat cgccaaagac tatcctgctg
     7261 cggtgacgct cggcccggtc acgctcgcgg ggaagtcgat cgccgctacc gtgacctggc
     7321 cgccagcacc gggccagacc ggcgacgcca tcgcctgacg atcgctgcgc acgccaggac
     7381 agtgatggct ggcagcgctc gggcggggcc gtgcagggtg acggtcgcgc ccgggagctg
     7441 ggcggcggcc tgggcgacgg ccagcagggc cgcgaggaat ggggcggcca ggcggcaggc
     7501 cacgacggcg agcggcggga cgaccggcgc ggcgagcgcg gcaaccacac cgaggagcat
     7561 cggcggagtc gccagcggga gcgcgaggag gttcgcgggc aggccggcga gggagagctg
     7621 cccgagtgcc agcgcggcgg cggggagcgc ggcggcctgg gcgccgagtg cgaagccggc
     7681 ggcggcgcgg accggctgcg gcagccaccg gggcagcgcg cgggcgattg gcggggccca
     7741 gccccaggac cccggcggtg gcggcgaccg acagctggaa gccgagcgtg gcggcgaggc
     7801 tggggttggc gagcaacagg aacatcacgg cgaggcagag ggcacggcgc ccgccggggc
     7861 ctcggccggt ggcgactccg gcgaggacca gggcggccat gacgctggcg cgcagcacgc
     7921 tcggctccca gcgggtgagc agcgcgaacg cgatggtcaa caggacgccg acagcggcga
     7981 gcgagggccg gccggccccg agggcggcgg cgagcccgag gcctgccgcg agcagcaccg
     8041 ccaggttctc accactggcg gcgagcaggt gggagaggcc cgcatccttg aagacacggt
     8101 cgagggtggg gctgagcagc gaggtgtcgc caagcgccat cccgaccagc agaccggcgt
     8161 gctcgggcgg caggctcgcc cgggcgctgc cacgggccgc ggcgcggaac gcctcgctcg
     8221 accgcagcag gcgcgagcgg gacggtgccc aagcctccag gtgtggccgg cgcagggtga
     8281 ccggcgcctg gtcgctgacg ccgatccggc tgggatgggc tgccggcgcg actcccgcgt
     8341 gcaggcggag ctgatcgccg accgcgaccg tgctgacgga cggagccgcg tcgcgtccga
     8401 agacgacggc cgcccgctct cgggtccgcc acgtccgccc ggcggtcgtg accctgtcca
     8461 ctcgcagcac caccaatcgg gcgccgtact ggacggtgcg gggttcctcc accacggtgc
     8521 ccgtcaccgc cacgcccctg ccgggcgtcg ccgccaggcg tgggagcagc ccgccgctgg
     8581 tcccagcgac ccgcatggcc gcgaccgcgg cgccggtggt tgccagcgtc accagcacca
     8641 tcgccccggc aagcaccggg cgttcagccg cttcggacag cggcatcgcc gcgagcccgg
     8701 cggcggcgag cgcgagcagg acggcgaggg gtgtcgcatg ggtgccggca gcgaccgcgg
     8761 cgcaccctcc cagcaacacc ccggcggcaa ccgtggcggc gagcgcgacg ccgtccgggt
     8821 ctgggtcacg gcccctgcgc caccgttggc cggaccgttg gtcgtcaggg cgcattggag
     8881 accgtcacca ggtccttgag tgtcgcgaag cgccggtcgc cgatgccggg aacctgccgc
     8941 aggtcgtcga cggaggtgaa ggggtgcgcg gcgcgatagg cgatgatccg gctggcggtg
     9001 acctggccga cgccagggag cgtgtcgagc tgctcggtgg tggtggtatt caggtcgagc
     9061 ggtccggacg gcggccctcc cagaccggtg gaaccggcgg ggtcgagctg cgcggtggtg
     9121 ggcaggacgg ccgcctcgcc gtggcggggg acgcgaagct gctcgccgtc ggccagcttg
     9181 cgggcgaggt tcaccgcgtc gaggtcggcg ccgggagctg ccccgccggc tgcctggatg
     9241 gcgtcgatga cccggctccc caccggcagg tgaaccagtc cagggtggcg cacccggccg
     9301 accacgtcca cggccagcgc gccggtcccg gtgtcggctc ccgtgccagc gccgaccgcg
     9361 ttggcgccca ttcccgatcc cggcggggtc ggcggcgtcg cgtccgcgcc ggtgacgctg
     9421 gtcagcatgg cgccggacgc ggacgcccgc gcgccggcca tcgtcgggtc gatcgggcgc
     9481 ggcgtgcccc ggaaccagat gaccgcggcg caggccagcg cgaccagcgc cacgaccgag
     9541 agcacgaccc gttggcgccc cacaccggcg agcaggcggc cggcgtggtc ccgcagtcga
     9601 gcggtcaggc ggtcgggtgg caggacaaag gaaggcggtg tcggcatgcg gctcctccag
     9661 atccggcggg ccttcgcggc ccgttcgtca gggtcgcgat gaccgtaacc gttctgaaga
     9721 atccgcgttt tcgttgtcca caggccccga tgtccaggcc cgaagccgag ccgcgcaacc
     9781 cagcgggcac gccaaaagcg cgcgcgtggg ggtccccctg attcaacgct atcggatcgg
     9841 ggcgggcggc gacggcgtcg tccacagccc cggctgggct cgtcagcgcg cggtgaccag
     9901 gttgacgagc ttgggcggga ccgtgatgac ccgggcgatc gggcggtcgg cgagcgcggc
     9961 cttcaccttc ggggaggcca gggcgagggc gcgcaggtca tcctcgccgg cgtcggcggt
    10021 gacctcgatg cggtcgcgga ccttgccgtc gacctggacg acgcaggtga ggcgctcgac
    10081 ccggaccagc tcggggtcgt agccgggcca ggcggccagg tggactgact cggtgtggcc
    10141 gagggcgtgc cacagctcct cggtgacgaa cggggccagc ggggcgagca tcaggaccag
    10201 ccgctcggcg gtgtcgcgct gctcggtggc gctgccgcga ccactgcgga cggcgtcggc
    10261 caggtcgttg gagagcgtca tcagcttggc gacggcggtg ttgaagcgga accgctccag
    10321 gtcgtcgctg accgcctggg tggcgcggtg ggcgtggcgg accagggctg ggtcgctggc
    10381 gccaccatgc ccggctccgg agtcggcggc gccgcggacg gcgcgccaga cccggtggat
    10441 ccagcgggcc atgccctcgg gcgagacgtc ggcccagtcg acgtcgtcct cgaagggacc
    10501 ggcgaacagc atggtgaggc gggcgacgtc ggcgccgtgc tcgtcgatga tggtggcggg
    10561 ctcgaccagg ttgccgcgcg acttgctcat ggcggcgccg tgcatcacca ccatgccctg
    10621 gttgagcagc gcccggaagg gctcgcggaa gccgaccagg ccgaggtcgt gcagaaactt
    10681 cacgaaaaag cgggagtaga gcaggtgcag gatggcgtgc tcgatcccgc cggtgtactg
    10741 gtgcaccggc atccagcggg cgacccggtc ggggtcgaag ggccgctccg ggtccaggcc
    10801 ggtgtagcgc aggaagtacc aggacgagtc gacgaaggtg tccatcgtgt cggtctcgcg
    10861 cctcgccggg gtgccgcagc gggggcaggc gacgttgacc cagtcactca ccgtggccag
    10921 cggggactcg cccttgggcc ggaagtcggc cacgtcgtcg ggcagcggga cggggagggc
    10981 gtcgtcgggg accggaacct cgccgcagga gggacagtgg atgatcggga tcgggcagcc
    11041 ccagtagcgc tgccgcgaga ccagccagtc gcgcaggcgg tagttgatgg cgaactcgcc
    11101 gcggccctgc gcggccaggt cggcggtgac gcgccggatc gcctcgccgg cggggatgcc
    11161 gtcgtagggg ccggagttga ccatgacgcc ctcgtggccc atggcctcgg tcatctggcc
    11221 ggggtccagg ggcggctcgc cctcgggctg gacgacgatc cggacgggca ggccgtagtt
    11281 gcgggcgaac tcgaagtcgc gctggtcgtg ggctgggacg gccatgatcg cgccggtgcc
    11341 gtactccagc agcacgtagt cggcggccca gacggggatc cgttcgccgt tgaccgggtt
    11401 gatggcgtaa gcgcccagga acacacccgt cttgtcgcgt tcggtggaga ggcgctcgac
    11461 ctcggtctcg cggtcgacgg tgtcgcggaa gcggcgaaag gccgcttcgt gctcagtccc
    11521 ggcgaccaac tgctcggcca gctggtgctc gggtgcgagc acgaagaagg tcgccccgta
    11581 cagagtgtcc gggcgggtgg taaagatccg cacagtctgt ccggtttcgg aaatgatgaa
    11641 atcgacgtag gccccaacgc tccgcccgat ccagttgcgc tgcatggtca ggaccttgtc
    11701 gctccagcgg tcctccagct cggccatgtc gtccagcagg gcctgggcgt actcggtgat
    11761 cctgaagaac cactgggtca gctcgcgctt ttcgaccagg tggccgcagc gctcgcagtg
    11821 accgttgatg acctgctcat tggccagcac ggtctggtca tgggggcacc agttcaccgg
    11881 cgaggccttg cgataggcga ggccgcgctc gtacaggcgc aggaagatcc actgcgtcca
    11941 gcggtagtag tccgggtcgc aggtgttgaa ggtccgtgac cagtcgaacg agatgccgag
    12001 ccgctggaag gtggcacgct gtgtgtcgat gttctggcgc gtccaggtct tggggtggat
    12061 cccgcggctg atggcggcgt tctcggccgg caggccgaag gcgtcccagc cgatcggatg
    12121 cagcacgttg tagccctgca tgcgcttcag gcgggcgatc acgtcgccgc cggcgaacgc
    12181 ttccagatgg cccatgtgca ggtcgcccga tgggtacggg aacatgtcga gcgcgtagaa
    12241 tcgggcacgt tcggtgtcat tctcgtcagc tcggtagagc gcccgctcct cccaggccgc
    12301 cgtccacttc ggctcaacct gctgcgggtc gtaacgctcg ggatccacgg tcttgcccac
    12361 cttctcggat tcggacgaaa aaaattcttg agcgggccga tcaggacccg ccccactact
    12421 ccgcaagcat aacagcaggc cagcggtggt tcgggaggcc acgcgcaagg ggacagcgaa
    12481 gcggatccgg gctgtgaccg acttgatccc tttgattgcc ctctggacac ggggccacga
    12541 caggtgcaat gttccccgcc atatcgtcga agtcgtacga gccggacgta gatcgcttcg
    12601 ggtggcaagc gggcagcgtc cttgaacgga gcgccactca gaggggaggc atcgtggtaa
    12661 cagcacgcaa tcaggtcgga ggtgcaagcc cgtaccggac ggtccggaac tggcagccgc
    12721 ctcgctcgta cgaaccacca ttccgtcccg ccccggcgac cccagccgac agcgaggagc
    12781 tcgaggtgct gctgcgcacc accgaggtcg cgcggcggct gcaggtgagc gcacggaccg
    12841 tgctcgcctg ggcccaggcg ggcaagctac cgagcatcct gactccgggg ggccaccgtc
    12901 gctacccggc gcagggcgtg gagcaggtgc tcgcgaccat gaccgcggcc ggggagccgc
    12961 gcgtcgacga gtaacggcag acgagtaacg gcaacggacg acaaccaacg accaaaacga
    13021 ccaacggcta gcgctgctga cgctacctct gggccgctgg gagcgcgcat gctggatcga
    13081 tgcgcgctcc cagccttccg gcggcgctcg gcggctcccg acgggtcgac ctgacggatt
    13141 gacccagtcg agggtctgat gcgtgaatgt tggtgcgatg gaaccagcga ctgaagggcg
    13201 cggcacgccg ctgagccttc ccaggaccgc ggcctgggtc tggcatgcgg agccacgctc
    13261 gatcttcggc ctcgacgccg tgttcgtgcg ttgcgcgaac ggcaccagca ccaccagtac
    13321 cagcggcgat ggcttcgact tcgctgacaa ctaccgccgc tggcaggaca cccgcggccc
    13381 ggcggtggtc ccgtggacct ggttcggccc ccccgccagc tccgacggga ccgctgccgc
    13441 cgacctgctc tgccagattg gcggagcgca accactgtat gtggtcgaga tcggcttcaa
    13501 ggcgcccgcc gaagaggtgg tcgccttcgc caagcgcatg cgcgagcgcc agcccgccgc
    13561 gctgctcggc ttctcgacct acccgaccag agccgaggca accgccaacg gcgtcccctg
    13621 ggacgtctgc gtggcagtgt tcgacctcgg cctcccgcag gtctacaccg ccaagcagcg
    13681 cctggagctg ctgcggccgg acagcccggt ggtcgccgac atggctggca agccgatcca
    13741 cgtggtggtc tgccccgaca gcgatcctgg ctggctggag gccgccaaag cggcgatggc
    13801 ccgccacgcg ggcgtctcgg cctgggcggt ggaccaggcc agcttcacca cctggcggcg
    13861 ccacctcagc ggcctggccg acgagcgacc ggccgccaag ccgcccagca ccagccggtc
    13921 cgcgctgacc gacgagctgc ccgccaccga ggccgccgag gaggccgccc cgccgtcccc
    13981 gtccccgctg agccccggtc cgggtccggt acccgcgccc acgccaatcg cggccggaga
    14041 tggcggtcca gcgccgattc ccacgccgcc gcgtcccgcg ggcctggagc cgggtgcgca
    14101 ggcactgctc gccaatcgca tcatcgcgat cgtccaggca cacctggacg ctggcggcac
    14161 cctcagcgac gaccagctcc tcagcgaggt ggaacgcgcg ctcggcgacg ccaccgctgg
    14221 ttgacagcct ccggtccagg tatccacaga tgccgacgag gcccttgatc ggcccgcgcg
    14281 gcgatgctct tattactgtg aggtaaccat ccaggtaacc ggccgggacc caacgcagga
    14341 cccggaggag gcgatcatgc aggcatccgc ggcgaccgag cgggccgcca tcgacgccga
    14401 ggtcagcggc aagacgctct gcgacctcct gctccgcaat gccgagacct acggcgacca
    14461 gccggcgctc tcctggaacg cggacggcga cggcgacggc ggctggcagc acctcacctg
    14521 gcgtggctat cgcgatcggg tcgccgaggc cgccatgggc ctggcggcgc tcggggtcgg
    14581 acgcggcgac ttcgtggcga tcatgacccg caaccgcccc gagcacctga tcgccgacct
    14641 ggccgcgctc cacctgggcg ccgtcccggt gtcgttctac aacaccctcg cgcccgagca
    14701 gatcgcctac atcgccgccc actgcgaggc caaggtggcc gtcctcgatg gccgccagtt
    14761 catggagcgc tgggagaagg tcaaagacga cctccccaac ctcacccacg cggtcctgct
    14821 cgaggacgcg gcggacttcg ccgactacgg ctgggtccgg tcctgggacg cgttgctgga
    14881 gggcggacgg gccgcgctcg ccagcgccga cggccgcgcg cgcttcgacg cggcctggcg
    14941 gcaggtgacc cccgacgacc tggtcacggt gatgtacacc tcgggtacca ccgggccgcc
    15001 caagggcgtc atgacgaccc actacaacgc gctgtggacc gccgcggcgg tcgagcgctg
    15061 gaccagggag tccaaccggg ccgcctggga gccgggcgcg acctacctgt cctacctgcc
    15121 actggcgcac agcctggagc gtctgagcgg ccactacgcg gcccagtggc gggccacctc
    15181 ggtctggttc tgcccggagg tgctccgggt ggtcgagtac ctcccgcagg tccgcccagc
    15241 caccttcgtg gcggtccccc gcctctggga gaaggcgcag gccggcatca ccgccgcgct
    15301 cgccgccgag ccgaacccgc gccgccgccg catcgccgag caggcgatcg ccagcggccg
    15361 ccaggcggtg cgcctgttcc aggcgggcaa gccgatgccg ctcgggctgc gcctgcgccg
    15421 cgccctgttc gagcggctgg tgttcagcaa gatccgccat cgcatgggcc ttgaccgctg
    15481 cctggtcgct ttcagcggcg ccgcccccct cgccgaggac gtgctggagt tcttcctggc
    15541 gatcggcatc ccgctgcggg agggctacgg cctgaccgag tcaaccgccc cggccagcct
    15601 caacctgccc gagcgggtca aggtcggcac ggtcgggccg gccctgcccg gggtcgaaat
    15661 gcgtgtcgcc gacgacggcg agctgctgat ccgcggcggc aacgtcgcca agggctacta
    15721 caaggaccct gagcagaccg ccgcgacctt cggcccggac ggctggctgc acaccggcga
    15781 cgtcgccgag gtcgacgagg acggcttcat ccgcatcgta gaccgcaaaa aggagctgct
    15841 gatcaccgct ggcggcgaca acgtctcacc atcgaatctg gagaacctcc tgaaggcgca
    15901 tccgctgatc gcccaggcgt gcgcggtcgg cgaccgcaag ccctacatcg ccgccctggt
    15961 cgtgctggac ggcgaggtgg cccccggctg ggcggggcag cacggcatcc ccttcgagag
    16021 cctggagcgg ttcgccctcg acgagcgcat ccgcgccgag ctgcagcagg cggtcgacca
    16081 ggtcaaccag cacgtcgccc gcatcgagca gatcaagcgc ttcaccgtcc tgccaaccga
    16141 gtggacgatg gactccgagg agctgacgcc aaccctcaag ctgaaacgcc gcgtgatcca
    16201 ccagaagtac gccgccgaga tccaggcgct ctaccagtag ccgttgcggg gggagggtgt
    16261 ggcgcctgcc gccgccccgg gcgggcacca caccctcggt ccgcgctcag tcccgcgggt
    16321 cagtccgcga tgcggagcgc gaccgccggc cgcagcctcg cggcctgccg tgccggccag
    16381 gcggtcgcca gcagcgacgc ggcggcggtg agcgacagca acaccgcgat gttcagccac
    16441 gggatcgaga acgccacctg cgcggccttg agggcggggt cgttgcggaa cagcaggtag
    16501 tcggtgacga ccgccagcgc cgcgccgagg atgatcccct cggccgcgat gaaggcgctc
    16561 tccgacagga acgcgcggtg gatgacgcgg gcctggaagc cgagcgcgcg cagcacgccg
    16621 acgctgcggc ggcgttcccg caccgcccgg accatcacca cgccgagccc gccgatgccg
    16681 atcaccagcc cgagcgccag gaacccttcc atcagctcgt cgaagctggt gttggcggcg
    16741 aactcctggt ccacctcgtg gcggatgcgg gtggcgacca gcccctgcgc caggaaggcg
    16801 ccctgtagct cccccgccag cgcctggtcg gagacgcccg gcgccggctt gaccagcacc
    16861 gcgctcagct tgagccacgc gccgaactgc gcccgcgcgg ctgggacgcc catgatcacc
    16921 ggcggcccgt cgttgcccgg gccgaagaag gcgtccgggt tgccgagcac gcccgcgacg
    16981 gtcttgcgcg caaccttgcc gctgcgcggg tcggtgaggg tgaagctgtc gcccggtttg
    17041 gtctcgccct tgccaccgag cagggcattg acggtgatca cataccgcgg gtcgttgagg
    17101 accgcccgcc acaccgcccg gtcgtcgccg aactggccca gccgcttgtc cagcgggaac
    17161 aggcccgccg tggcgatccg cgggtcggcg ccgaccacgg tgacgcggtc ctggcccgat
    17221 tccccgcctg gctgcgcctg gccggccgct cccggcgcct ggatccggcc gtcggcggcg
    17281 gagaggggca ccgccgcggc caccttgcgc gcgaactggc cggtggtgaa cgccgcggcc
    17341 gggtctgggg ttgtcaccgg cgccgagggg ttgaggtcgg cgcggatcgc gaacccgccg
    17401 gtggcgctgg cgacctcgtg cgccctggtc gagtcctcaa cgctggtgag caccgccacc
    17461 agcacgatcg tgaacaccac aagcccgtac atcaccagga tcgcgccggt gcggaagcgg
    17521 cgcgccagcg ggtaggcgaa ggcgagccgt gctgccagcc cccacggcgt cgcccgggac
    17581 gccagcggcc gcagcggccc ggtgaccagc tgctggttct ggctcagcag gaacaccgcc
    17641 gagaaggtca gcgccacgcc gagcaccacg taggtgcccg tggagccgtc gtcgaacagg
    17701 tgcggccgca cggtgttggc ggccagggtc cagccgagca cgccgagcgc cgccaggctg
    17761 taggcgagcc gcttgggcag cagccgcacc agcagcgggc acagcgccag cgcggccagc
    17821 gccgggagca ggtagaccag cgagccccgg ccgttggcga ccaccggcac cgtcagcacc
    17881 ccgagcccgg cggccaccag ggtcgacagc gcaacccagc gccgcttgag gcgccgcccg
    17941 ccgtcgcccg gcaggtcgcg aatcgcggcg atcacgttga ggcggctgac ccgcacggcc
    18001 gtcagcgtgg ccgtcaggaa ggcgatcagg aacccgaacg ccaggccgtt gacgacgctg
    18061 accggggtga tgtggaacac gatcgccagg ccgccctgat cgctggagaa gctgccgaag
    18121 atgcgccccg cgaagatccc gacggcgcgg ccgacgccga tcccggcgag cgcgccgatc
    18181 acgcaggcga tcagcgcgta cagggcaccc tcgatcatga aggcgcggac cagccgcccg
    18241 cgccgcatgc cgatggcacg caggataccc agctcgccct tgcgctcatc ggcgagcatc
    18301 acgaacacgt tcaccagcag catcactccg gcgatgatcg agaagctgcc gatgaacagg
    18361 aagaacgaac cgaactggtc gccggctgac ttggcgtcgg ccagggcatg ctgcttgacc
    18421 gtatggaccg cgaccgcgtc ctcgcccgtg cccgtgcccg tggccttggc attggccttg
    18481 gcggcgagtg gggcgagggc gcgggtcagc ttgcccgcga ccgcgtcgct gtgcgcgctg
    18541 ccgccttcga tgtcgccggt gttggagacg aacgtcacca cctccggctg cgcgtccttc
    18601 ggggcgagcg ccgccagcgt ccccggcgcc aggaaggcgt tgcgcccgcc gttgccgccg
    18661 gcgccggcga tgcccgtcga gccgaccacc tgggtcaccc gcagcgccgt cgggtggccg
    18721 tagagccaga aggtgagcgt gtcgcccgcg ctggcgccga gcgctcccgc cagctcgctg
    18781 ttcagcacca cctcgccggg gttgggggtg ggggcgtcca ggccggcgcc tccgtcgctg
    18841 ccagcgacgg gctgggcggt gccgaagtcg gcttcggcca cgttggcgtt cggctcggcc
    18901 ttgcgcgcgg cgccgtcgcc cttggccacg gcggcctggt cgatccggag ggtgaggacg
    18961 ccgtccacgt ccgggtcgcc gcgcagcacc gcgacccgtt tggcggcggc ctcgccctgg
    19021 gcgggggtcg gggcgctgac gatctcgtcg atcgggccga gctggcggtc ggcgtcccgt
    19081 ttcaccgaga agccaagggt gtcgccgacg atcaggctgc cgacgatgat cgcggtgccg
    19141 agcgccgagc cggcgatcac cagcagcgcc tcgccgcggc gccggctcgc ctggcgcagc
    19201 gccaggcggc gcaggaacgg ccgccgcagg gcgaccagcg ccgtcgccag cagcgccgct
    19261 gccatcagag tgatcagcgg cagggtcagg ttcggataca cgcgtcagcc ctccagaccc
    19321 tggtgctgac cggcgtaggc cggtgggcgc ggctcgtcgg cgaccacgcg gccgtcgcgc
    19381 atgcggacgc ggcgcccggc cgccgcgccg atcgccgggt cgtgggtgac cagcacgacc
    19441 gtctggcctt cgtcacggtt caggtcctgg agcaacgcca gcacctgggc ggccatctgc
    19501 gtgtcgaggt tcccggtcgg ctcgtcggcc cacacgatcg ccggcctggc cgcgagggct
    19561 cgcgcgatgg tgacgcgctg ctgctcccca ccggacagct cgttgggccg gtggcgcagg
    19621 cggtggccga ggccgacccg ctccagcatc atcgccgcct cgctccgtgc ctgccggggc
    19681 ggcgcgccgg ccagcagcag cggcagctcc acgttctcgg cggcggtgaa caccgggatc
    19741 aggttgtagg cctggaagac gaagcccatc gccctggccc ggtaggcagt ccgcttggcg
    19801 tccgacagcg cgaacagatc ctggccttcg accagcaccc ggccgctgtc gatgccgtca
    19861 agcccggaca ggcagttcag cagggtggtc ttgcccgacc ccgaggggcc catcaccgcg
    19921 accagctcgc cccggtcgat ggcgaggtcg aggccggcga gggcctccac ctcgatctcg
    19981 ccgccatcgc cggtgcggta gaccttgcgc acgccctcgg ccagcagcaa cggctcgccc
    20041 attgtccctc ccgtcgatca cgcgacccga gacccgattg gtggccgcgg tcctgacggc
    20101 caggctcgct gctggcgggt gggggcacat cggggaggct ccctgaacga acctgcggct
    20161 atcccccgtt ttctcagggt ttcccctgag acgggcgggg ttggtcagct gtagctcgtc
    20221 cgagactcgg tctcgcgggc gatgccttcg gcgacgctgt cggcgacgct gcgggcctcc
    20281 catttgagcg cgggtggcac acgcagctcg aagcgggcgc ccgcgccggt ccgggtcggc
    20341 tcggccatca ccaggtcgcc gccctcggcg cgggcgagcc gccgggcgat gtagaggccc
    20401 agcccgacgc cgtcagcatg gcggacgacg ttgccgtcga gcttgccgaa gcgcccgaag
    20461 atccactccc gcttggcggc tggcacgcct gggccaccgt cctcgacggc gatgactacg
    20521 aggttgccgt catggaacgc gtcaacccaa accggcgccc cagacggggc gtacttggcg
    20581 gcgttgtcga gcaggttgga aaccgcctgc aggaccgcct cgggcgcggc atgcaccggc
    20641 aggtcgtcct ccacgcccaa ttccatcacg cggcgggggt agcgcgcctt ggccgctgcg
    20701 accgcctcgc gagccagctc gccagcgtcg accggcgggt gccgattggg cagtggcacg
    20761 ccggacgcct ggacctgcgc ggcgagtagg agatgctcga tcagctcctt gagccgctcc
    20821 ccctggcgga cggccatgcc gaccagctcg cgcttctgct cgtcgctgag ccgctcctca
    20881 cggttctcca gcgtctgcac cgctcccagc atcccggcga gcggcgtacg gagctcgtgc
    20941 gaaaccgtgg agagcacgtt gtccttgaac tggtcgcttt gcagcaggcg tcgcaccacc
    21001 tcctgctcgg cctcgaccaa ccgcgccttc tcggccgcga tcttgcgggc ctcctcggcg
    21061 gccgcttcag ccacggcacg gttggcgtgc gcgtctgccg ccgccttgta cgccagatgg
    21121 actccgatca tcggaagtgc cagcagcaag gctgtggcag caccttgtct ggcgaggatg
    21181 accgcgaccg gtgccatccc aaccaggagc gcctcgggca gcgcggcgag cccgatcggg
    21241 tcgaggatct gaggagcgag cggcgctcct tggcgaagtg cgattacgat cctgaccagg
    21301 attcgattgg cggcgaagaa ggccatgccc gtaaggagca acgccaccaa gccccggatg
    21361 gagagctgcc actcagcgcc actgcccacc aactcaacga cggcgctggc cgcagccagc
    21421 gcgaggacgt actgcgcagc gttgaacagc gtccgcatga gcgccttacg gcgtagcaga
    21481 tccgcgatga tggagccggc taagacgaca agtgcggctg gcccgagccc ccaccccaag
    21541 agcagcgcca ccgtgaacgt ggtggaggtc gtgaattcct cgacctgccc acgccgcagg
    21601 atgaagatgg gccggacctc acccaccgtg aacaacacag caagcagcca aaaggcggct
    21661 ggggcgcgct gtacgtcggc gaggccacca cgagcggcga gcaggtagac gactagtgcg
    21721 cccgccgtta tgacagcacc gacgtagctg tggaacgacc agcggtgggg acgaccggtc
    21781 attgccctcg atctcgcgag tcaggacagg gagggccata gcagaccact tcgccccggg
    21841 tcttgtcgag tcccggggcg aaggcggcaa atcagtaaac cgtcgctacc acttctgtcc
    21901 ggcgcccagc gcagcggcga gcgcaccaaa cgtggcgagc agcaatgcgg cacgaacgta
    21961 ccgacgcttg aggaaggcca cgacccttca cctcctcgtt gtgatgcacg tgcgaccggg
    22021 gaccgggctg aagtcgcgac ccgacagttt cttgggcgag gaggatctga aggccccccg
    22081 gcattgccag attatatcaa acccgcacaa gcgactcaac cagtcgggcg tatcaaactt
    22141 ggtaagacgc ttgaagcgct cctacctcgc tcacctcatg cattcttcgt agatatagcc
    22201 tacatcatag gaactagggc tgattctccg aacggtattt gcccacctgg accgccccga
    22261 aacgggcgcg atccccgtag gggcaagcgg cacggctgtc gcgggcgtga taaagaatcc
    22321 acctcatgtc gttctacgcc gaccttcaca tccactcgag gttctcaagg gcgaccagcc
    22381 gggacctcga cctcgaacaa cttgcctggt ggggacgccg caagggcctc tcgatcctgg
    22441 gcaccgggga cttcacccac ccggcgtggc accagcacct gcgcgaggag ctggtgccgg
    22501 ccgagcccgg gctgtaccgg ctgcgccccg acctggacgc cgcggtcacg cagcggctgc
    22561 ccggctcg
//
LOCUS       DASWDC010000218         5254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_57428, whole genome shotgun sequence.
ACCESSION   DASWDC010000218 DASWDC010000000
VERSION     DASWDC010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57428"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VF995_07115"
     CDS             <1..430
                     /locus_tag="VF995_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX9343368.1"
                     /translation="LLDTTFWGGMRACVKAAAVCETFQLGIAVHSSGELGVQLATMLH
                     LGAALPNLTLAADAHYHHLRDDVIEGGKLPYTDGRIRVPTGPGLGVRLDRERLARYAE
                     LYRELGGYPYDRDPARPGWYPLIPNDRWADPEVAATPDLG"
     gene            complement(602..1072)
                     /locus_tag="VF995_07120"
     CDS             complement(602..1072)
                     /locus_tag="VF995_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9343369.1"
                     /translation="MDTLDRAILVQLQEDGRLSNAELAERVGLSPSPCLRRVRNLEAD
                     GVITGYRAVLDPAKVGSAFQVLVHVTMAVGDRKATMEAFEAGVAAIDEVVECRRMFGD
                     PDYLLWVAVADLEAYERLYMSELVGLRGVARTSSQLTLKTIKPGGRLPLPPRSR"
     gene            1208..1588
                     /locus_tag="VF995_07125"
     CDS             1208..1588
                     /locus_tag="VF995_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9343370.1"
                     /translation="MIQGISKVVVPVDDQERAKRFWTERMGFTLTTDEAYGDERWVEV
                     TPPDKTVTLVLALRQPSEPRPEVPETMPHSPVMFACDDIQRTHQELRDRGVGFPTPPT
                     EMPFGWWSLFEDDGGTRYALEQRS"
     gene            complement(1644..2438)
                     /locus_tag="VF995_07130"
     CDS             complement(1644..2438)
                     /locus_tag="VF995_07130"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014528180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9343371.1"
                     /translation="MGEFSGKVALVSGGSLGIGRATVERLAEAGAEVVFCGVGASVGE
                     AEAALRDRGLAVRGVEADVTVAADVERLVAVAVERHGGLDVVANCAGIQRYGTVEETD
                     EAEWDRVLAVNLKGIFLVCKAAVPELRRRGGGAIVNLSSVQAFQTQTGVAAYTASKGA
                     INALTRAMAVDHARDGIRVTAVCPGSVDTPMLRWAADQFRGDRPAADLVADWGRMHPL
                     GRVATPAEVAELVAFLASPRASFITGSAHLVDGGLLARLAVALPDR"
     gene            complement(2540..4570)
                     /locus_tag="VF995_07135"
     CDS             complement(2540..4570)
                     /locus_tag="VF995_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9343372.1"
                     /translation="MSEVEAPPHREILVAFSGLVLGMLLAALDQTIVATALPTIVGDL
                     GGLDHLSWVVTAYLLASTVSTPLWGKLGDLYGRKPLFQGAIVLFLAASALAGLSRGMD
                     QLIAFRALQGLGGGGLLVTAQGIVGDLAPPRDRGRYQGIFGAVFGLASVAGPLLGGFF
                     VDNLSWRWVFYVNLPVGAVALVVTTLVLRQPERRVQHTVDYLGTVLLGGAATCLVLLT
                     TFGGTTYPWRSWQIAALGCGVGSLLLGFVAAERRAAEPVLPLGLFQSRVFSLAGAISF
                     VVGFGLFGAVTFLPLFLQIVQGASPTVSGLRMLPMMLGLLFTSIASGQLISRRGRYRV
                     FPIAGTAVLAVGLLLLSRLEPSSSVLRSSLGMLVLGLGLGMVMQVLVLVVQNAVEYRD
                     LGAATSATTFFRSVGGSFGVAVFGTVFASRLQANLHSLLPASAAAQLGAAGTSLQPSQ
                     LAGLPAAVRSGVELAYSNSIQSVFRSALPVAVLGFLLALLLPERPLRPTAGATGSGET
                     FAMPASPSSLEEIQRALSVLARRENHRVVYQSLAAKAGLRLDPAACWLLLRVGEHEGS
                     DADALVERLRVPVRALRRHLGRLMGAGLVTLAGEHEPLALTGAGHSAVDQLVAARRDC
                     LAEQLADWSPECRAEAAEFIARLASDLLSDPTGKGGELVAGSHGRRLTRSAR"
     gene            4884..>5254
                     /locus_tag="VF995_07140"
     CDS             4884..>5254
                     /locus_tag="VF995_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017596852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HEX9343373.1"
                     /translation="MEPNGGKPTAKPTARWRTWLRLALVALFLVFVVVLLVKQWPQVR
                     PLLRRLDPAAIVAATAAVLAGVFGTFRAWRAVLADLGSPLPQAAGMRVFFLGQLGKYL
                     PGSVWPVLAQMELGRDYEVPER"
BASE COUNT          813 a         1909 c         1880 g          652 t
ORIGIN      
        1 cctgctggac accaccttct ggggcggcat gcgcgcctgc gtcaaggcgg cggcggtctg
       61 cgagaccttc cagctcggca tcgccgtcca ctcctccggc gagctgggcg tgcagctcgc
      121 caccatgctg cacctcggcg ccgccctccc gaacctgacc ctcgccgccg acgcccacta
      181 tcaccacctg cgcgacgacg tcatcgaggg cggcaagctg ccctacaccg atggccgcat
      241 ccgggtcccc accgggcccg gcctcggcgt ccgcctcgac cgggagcgcc tcgcccgcta
      301 cgccgagctg taccgtgaac tcggcggcta cccctacgac cgcgaccccg cccgccccgg
      361 ctggtatccc ctgatcccca acgaccgctg ggccgacccg gaggtggcgg ccaccccgga
      421 tctcggctga ggtcagttcg ccctgcgacg ttccgcgcag agctgtggat aaccctgtgg
      481 acaagtaccc ccgattctgt ggatgagcgg ttcggggtct gtagacaaag agctaaagcc
      541 tgtgggcagc tcccgccagc ctgtggataa cctgcgcctc cattgacacg tagcgggcca
      601 ctcagcgtga ccgcggtggc agcgggaggc gtccgcccgg cttgatggtc ttcagcgtca
      661 gctgggagct ggtacgggcc acgccgcgga ggccgaccag ctcgctcatg tagagccgct
      721 cgtaggcctc caggtccgcg accgccaccc acagcaggta gtcggggtcg ccgaacatcc
      781 gccggcactc gaccacctcg tcgatggcgg cgacgcccgc ctcgaacgcc tccatggtgg
      841 ccttgcggtc cccgaccgcc atggtgacgt ggaccagcac ctggaaggcg ctgccgacct
      901 tggcggggtc gagcacggcc cggtatccgg tgatcactcc gtcggcttcg aggttgcgga
      961 cgcggcgcag gcacggcgag gggctgaggc ccacccgctc ggccagttcg gcgttggaga
     1021 ggcgcccgtc ctcctgcagc tggacgagaa ttgctcgatc aagcgtatcc atagcagaag
     1081 atgctaacac aaggcaatca tacggcagaa tttggcacca ccttcgcctg attctgcgct
     1141 acggttgcgt cacggcgagg cgccagctcc gccgggaacg tggccaaacg gcagcgagga
     1201 ggccgccatg atccagggga tcagcaaggt cgtcgtgccc gtggacgacc aggaacgcgc
     1261 caagcggttc tggaccgagc gcatgggctt cacgctcacg accgacgaag cctacggcga
     1321 cgagcgctgg gtcgaggtca ccccaccgga caagaccgtg accctggtgc tggcactgcg
     1381 ccagcccagc gaaccccgcc cagaagtccc cgagacgatg ccgcactcgc cggtgatgtt
     1441 cgcctgcgac gacatccagc ggacccacca ggagctgcgc gaccgcgggg tcggcttccc
     1501 aactccgccg accgagatgc ccttcggctg gtggtcgctg ttcgaggacg acggcggcac
     1561 ccgctacgcc ctcgagcagc gctcctgacc cggccggcag agcagcgggg catggccgtc
     1621 gtgaagacca ggctctgccg ggctcagcgg tccggcagag ccaccgcgag acgggcgagg
     1681 aggccgccgt cgaccaggtg ggcggagccg gtgatgaagg aggcgcgggg gctggcgagg
     1741 aaggcgacca gctcggcgac ctcggcgggg gtggcgaccc ggccaagggg gtgcatgcgg
     1801 ccccagtcgg cgaccaggtc ggcggcgggc cggtcgccgc ggaactggtc ggcggcccag
     1861 cgcagcatcg gggtgtcgac cgagccgggg cagaccgcgg tgacgcggat gccgtcgcgg
     1921 gcgtggtcga cggccatcgc gcgggtaagg gcgttgatcg cgcctttgct ggccgtatag
     1981 gccgcgaccc cggtctgggt ctggaacgcc tgcaccgagg agaggttgac gatcgcaccg
     2041 ccgccgcgcc gccgcagctc ggggacggcc gccttgcaga ccaggaagat ccccttgagg
     2101 ttgaccgcca ggacgcggtc ccattcggcc tcgtcggtct cctcgacggt cccgtagcgc
     2161 tggatgccgg cgcagttggc caccacgtcg agcccgccgt ggcgctccac cgcgacggcg
     2221 accaggcgct ccacatcggc ggcgacggtc acgtcggcct cgacgccgcg cacggcgagc
     2281 cccctgtccc gcagggcggc ctcggcctcg ccgacgctgg cgccgacgcc gcagaagacc
     2341 acctccgcgc cagcctccgc caggcgctcg accgtggccc ggccgatccc gagggacccg
     2401 ccgctcacca gcgccacctt gccgctgaac tcacccatgc ccagcctgcc tcctcctgcc
     2461 gcagcgtcca cccgcggcgt ccacgtctgg tctcgcgcag caagcctagc ggcaaggccg
     2521 cccactggga ccgggacggt taccgggcgg agcgggtgag gcggcggcca tgggagccgg
     2581 cgaccagctc gccgcccttg cccgtggggt cgctgagcag gtcgctggcg agccgcgcga
     2641 tgaactcggc ggcctcggcg cggcattccg gcgaccagtc ggcgagctgc tcagcgaggc
     2701 agtcgcggcg cgcggcgacc agctgatcga ccgcgctgtg gcctgcgccg gtcagggcga
     2761 gcggctcgtg ctcgcccgcc agcgtgacca ggccggcacc catcaggcgg ccgagatggc
     2821 gccgcagcgc cctgaccggc acccgcaggc gctcgacgag cgcgtcggcg tcgctgccct
     2881 cgtgctcgcc gacccgcagc agcagccagc aggcggcagg gtccagccgg agcccggcct
     2941 tggcggcgag gctctggtag acgacacggt ggttctccct gcgggcgagc acgctgaggg
     3001 cccgctggat ctcctccagc gaggagggcg aggccggcat cgcgaaggtc tcgccgctgc
     3061 cggtcgcccc ggcggtgggg cgcaggggcc tctccggcag cagcagggcc aggaggaacc
     3121 cgagcaccgc gacggggagc gccgagcgga acaccgactg gatggagttg ctgtaggcca
     3181 gctcgacacc cgaccgcacc gccgccggga gccccgccag ctgcgacggc tgcaggcttg
     3241 tcccggcggc gccgagctgg gccgcggcac tggccggcag cagcgagtgg aggttggcct
     3301 gcagccggct ggcgaagacg gtgccgaaca ccgccacgcc gaacgacccg ccgaccgagc
     3361 ggaagaaggt cgtcgccgag gtggcggcgc cgaggtcgcg gtactcgacg gcgttctgca
     3421 ccaccagcac cagcacctgc atgaccatgc cgagcccaag gccgagcacc agcatgccca
     3481 ggctggagcg aagcacgctg ctgctcggct ccaggcgcga gagcagcagc agcccgaccg
     3541 ccagcaccgc cgtcccggcg atcgggaaga cgcgatagcg cccacggcgg ctgatcagct
     3601 gtccactggc gatcgaggtg aacagcagcc ccagcatcat cggcagcatg cgcaggcccg
     3661 agacggtcgg cgaggcgccc tgcacgatct ggaggaacag cggcaggaag gtgaccgcgc
     3721 cgaacaggcc gaagccgacc acgaagctga tggcgccggc gaggctgaag acccggctct
     3781 ggaacagccc gagcggaagc accggctcgg cggcgcggcg ctcggcggcg acgaacccga
     3841 gcagcagcga ccccaccccg cagccgagtg cggcgatctg ccacgagcgc cacgggtagg
     3901 tcgtcccgcc gaaggtggtg agcagcacca ggcaggtggc ggcaccaccg agcagcacgg
     3961 tgccgaggta gtcgacggtg tgctggacac gccgctcagg ctggcgcagc accagcgtgg
     4021 tcaccaccag cgccaccgcc ccgaccggca ggttgacgta gaagacccag cgccaggaca
     4081 ggttgtcgac gaagaagccg ccgagcagcg gtccggccac gctcgccagc ccgaacaccg
     4141 cgccgaagat cccctggtag cggccccggt cgcggggcgg cgccaggtcc ccgacgatcc
     4201 cctgggcggt caccagcagc ccgccgccgc cgaggccctg caaggcccgg aaggcgatca
     4261 gctggtccat gccgcggctc agcccggcca gcgccgaggc cgccaggaac agcacgatcg
     4321 cgccctggaa caggggcttg cggccataca ggtcgccgag cttgccccac agcggcgtgg
     4381 acacggtgga ggccagcagg taggcggtga ccacccagga gagatggtcg aggccgccga
     4441 ggtcgccgac gatcgtgggc agcgcggtgg ccacgatcgt ctggtcgagg gcggccagca
     4501 gcatgccgag caccaggccg ctgaacgcca ccaggatctc gcggtgcggc ggcgcctcca
     4561 cttcgctcat ccggctcgcg ctcccatcgt tatcgatccg ttgccgctgc tgtggtcatc
     4621 ggatcggcac gctggcgagc tgagcgaaag ctccaaaggc tcaggaaacg cggcgaacga
     4681 ctggcgaggc gggaggttcc gaacaggaac gcgctcagcg gcatccccca actccgccgc
     4741 gagctcgcgc gcccccagtc gcgcggcctg ttctccgtcc cgcctcgccg ggtggttcgg
     4801 tgctgccggc ttctctaacc cttcccaccc gagggcgagg cccaaaccag ctcgattgac
     4861 cgcccgcagg ataggatgcg cggatggaac cgaacggggg gaagccgacg gccaagccga
     4921 cggcgcgctg gcggacctgg ctgcggctgg ctctggtggc gctgttcctg gtgtttgtgg
     4981 tggtgctgct ggtcaagcag tggccgcagg tccggccgct gctgcggcgc ctcgacccgg
     5041 ccgcgatcgt ggcggccacc gcggcggtgc tcgccggggt cttcggcaca ttccgggcct
     5101 ggcgggcggt gctcgccgac ctcggctcgc cgctgccgca ggcggccggg atgcgggtgt
     5161 ttttcctcgg ccagctgggc aagtacctgc ctggctcggt ctggccggtg ctggcacaga
     5221 tggagctggg gcgcgactac gaggtgcccg aacg
//
LOCUS       DASWDC010000219        12069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1225563, whole genome shotgun sequence.
ACCESSION   DASWDC010000219 DASWDC010000000
VERSION     DASWDC010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12069
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225563"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VF995_07145"
     CDS             complement(<1..1239)
                     /locus_tag="VF995_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9343374.1"
                     /translation="MAEAEAQAERRGLGTGTAPYPFSALVGQPDLTLALLLNAVSPAI
                     GGVLVRGEKGTAKSTAVRGLARLLPEIEVVGGCPFNCDPAAPDPACPAGPHPADAPAK
                     RRPVPLVELPVGASTDRVAGSLDLEQALGEGRRVFEPGLLAAAHRGILYVDEVNLLGD
                     HLVDLLLDAAALGTNYVEREGVSVRHAARFLLVGTMNPEEGELRPQLLDRFAVTVEVR
                     GNAAVADRVEVVRRRLAYEADPAAFAAAFAGADAALTGRIAAARARLAAVQLPDELLA
                     RIAEVCAAFGVDGLRGDIVCAKTAMALAAWAGRDQVEVADVRSAALLALPHRRRRGPM
                     DSPGLDERELDRILGAGPDDDDPDPAGPGGSRPNRPDGSDGSDGNDAGAAPGDGQRGA
                     LPPHGGRPGDAQAGPALDPAT"
     gene            complement(1232..4975)
                     /gene="cobN"
                     /locus_tag="VF995_07150"
     CDS             complement(1232..4975)
                     /gene="cobN"
                     /locus_tag="VF995_07150"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018587226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEX9343375.1"
                     /translation="MILFLTTADTEILAARRARATLPGAFPPVRAANPTADPASPATV
                     ELGEAKVVLVRLLGGRRAWPAGFDQLAADCHAAGMPLLAFGGEAEPDAELAAASTAPA
                     GVVAEAFEYLRHGGVANMANLFRFVADRLLYGGFGFEPPAVLPEAGLYRPELLDRHQP
                     ERPTVGVVFYRTHWMSGNTAFVDTLVEALEAAGANPLPLFCYSLRPDPTGRVAACELL
                     AAHSADALIVTVLAAGGSHAADAADPDGWQVPALDALDVPIVQGICATSTRAAWLASR
                     GGLSPLDAAMQVAIPEFDGRIIGVPFSFKEPIGGEVSAPGAAHNDSGASRSDSGALVY
                     RADPERAAALAAIATRLARLRHTPNAAKRVALMLSNYPTKHARVGNAVGLDTPASALR
                     LLAALRDAGYDTGEVPTDGDALIHRLIAAGGHDSEFLTEGQLAGASGRIDAAAYARWF
                     QTLTEPLRAAVVRHWGEPPGELYVDGDAIVLAGLCLSEHLFLAIQPPRGFGENPIAIY
                     HDPELPPSHHYLAAYWWLRHEDGFGADAIVHLGKHGTLEWLPGKGLGLSAACAPDAVL
                     GPLPLVYPFIVNDPGEGTQAKRRAHAVVIDHLVPPMQRAETYDDLARLEQLLDEHAQV
                     QALDPAKLAEVRGRIWELVRTAELHRDLGVEAPPGDDSFDEFVLHLDGYLCEIKDARI
                     RDGLHILGQAPAGEQRLGLLAAILRLGSAGLPGLRAAVAAAAGLDEAALLKAGDRQRL
                     DQLEAASMALLAAMDEARWDPEQAAAVTSRILHPDPEAGIQLEAAAAAVARVLAFAAR
                     EVVPRLERTSDELVNLVRALDGRFVPAGPSGAPTRGRVDVLPTGRNFYSVDPKGLPSE
                     LAYATGRRLADDLLRRWRTDRDQGQAGIEADGQGDWPETVGIVVWGTSAMRTQGDDIG
                     EILALLGVRPRWNPESRRVTGLEAVPPGELGRPRIDVTVRISGFFRDAFPNLVALLDD
                     AVTLVAGLDEPDEVNYLAKHARADRAAGADERRATTRVFGSKPGAYGAGLLPLIDARN
                     WRTEADLVKVYEVWGGYAYGRGLDGAPALEAMRTNFARIRVATKNVDTDEHDLLDSDD
                     YFQYHGGMVAAVRALGGAPHAYIGDSADPARVRTRTLAEEVRRVVRARVLNPRWIASM
                     RRHGYKGAFELAATVDYLFGYDATTGVVEDWMYQAVSERYVLDQENGAFLRTANPWAL
                     RAIAERLLEAADRKLWDAPDPETIGRLQATYLELEGELEERGG"
     gene            complement(4972..6489)
                     /locus_tag="VF995_07155"
     CDS             complement(4972..6489)
                     /locus_tag="VF995_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX9343376.1"
                     /translation="MSGALLVAGTASDAGKSVVVAGICRWLARRGLKVAPFKAQNMAL
                     NSAVTPDGAEIGRAQVAQAAAAGATPEAAMNPILLKPAGERVSQVVVMGEPFTTVDAR
                     SYQQLKPRLRGVVLEALADLRQRFDVVVCEGAGSPAEINLRDGDLVNMGLARAAGLPV
                     VVVGDIDRGGVFASLFGTLALLDAADQALVAGFLINKFRGDQAILAPGLEQLRALTGR
                     PVLGVLPWRRGLWLDVEDSLALEAPRDGALPPLGADSLVVAVARLRWLSNFTDFDALA
                     AEPGVVVRFTQSPAELLSADLAVLPGSKATVDDLAWLHARGLDQAIATRARRGLPTLG
                     ICGGYQMLGGRIADRDAVESDAGEVAGLGLLPVETVFAATKTLANPTGTAPAYGGVPA
                     AGYEIHHGRIRRFGGEPVLASTSGDQGCRQGTVAGLAWHGIFEGDAFRRAFLTWVAAE
                     RGLDWIPGDRPFAAVRAERLDALGDLIAEHADTAALDRLIEHGAVPGYPQVGGAR"
     gene            complement(6486..7460)
                     /locus_tag="VF995_07160"
     CDS             complement(6486..7460)
                     /locus_tag="VF995_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX9343377.1"
                     /translation="MARTRGVAPAGLLAGALADQFLGDPRRGHPVAGFGQAAVVLERR
                     VWRPSRAAGTAYTLVLVGGAATAAALADARLRQRPLARALLLAGLTWTALGGRSLART
                     ARQLGEAVREGDLERARQLAPSLVGRDPSRLDGVELCRAAIESVAENTADAVVGPLLW
                     GALAGPAGVAAYRAANTLDAMVGHRSSRYLRFGWAAARLDDLLTWPAARLGAVLACLL
                     AATGASDGDRWRAWRVLRRDGTAHPSPNAGRMEAAFAGALGVRLGGRNRYGDQVEQRP
                     TLGDGPAPGPDDVDRAVRLSRQVSVATVALAVALAALTTALRQRKRSR"
     gene            complement(7460..8104)
                     /locus_tag="VF995_07165"
     CDS             complement(7460..8104)
                     /locus_tag="VF995_07165"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9343378.1"
                     /translation="MARLILLRSAATAATLRAAFPDDEPLVARGERDAGALTHVPCLQ
                     PRRAGQVLTSPALRARQTAAAAGLDATVEEALADADAGAWRGLTLDEVADRDPERLAA
                     WLEDPDARPPGGESRRDLLTRVSACLDQLHTRILGSDSNGDEDQDGGGIRTGAARGAT
                     VAVTHSGWVRAAALLVLGAPPAAFWRIDIAPASLTVLHRRERGWALARVNWTAT"
     gene            complement(8110..8880)
                     /locus_tag="VF995_07170"
     CDS             complement(8110..8880)
                     /locus_tag="VF995_07170"
                     /inference="COORDINATES: protein motif:HMM:NF021039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HEX9343379.1"
                     /translation="MASLLRRGLIAGLLAGLVAGVFYLLVGEPVIQQALHYETVPAGT
                     HTMQVFSRSTQRVGLVVGVSLFGIALGALFTVVYALLGPRLAAHSGWDRSLRLAGVCF
                     AALWLVPFLKYPANPPGMGSAATAPERQRLYLIMLAVSVGAAVGAWLLARWLARDGMR
                     RETRQVLVGTAYLAVVAGAWVLLPDTAEATALPAALLWQAREVSAAGAALLWLLLGVS
                     FGWLSARAEQRQRQAGSATDSERDGAAAPVERPRLAGS"
     gene            complement(8944..9198)
                     /locus_tag="VF995_07175"
     CDS             complement(8944..9198)
                     /locus_tag="VF995_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB-domain containing protein"
                     /protein_id="HEX9343380.1"
                     /translation="MASPTSAAAAIPGPSASPSPSRPLGVPVPWWAWVAVGLVALLVF
                     AIGYDQGQLLEPALGKAAASGNYLHEFFHDARHLLGFPCH"
     regulatory      complement(9231..9423)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(9477..9983)
                     /locus_tag="VF995_07180"
     CDS             complement(9477..9983)
                     /locus_tag="VF995_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9343381.1"
                     /translation="MPDQTSRTRDEEAVRRFVEHMAMQFAEWGFARMAARVLMTMMSM
                     DEPSLSAADLAERLDVSPAAISGAVRYLMQLGLLAREPVPGTRRDRYRLPDDPFYEAN
                     IVRRGMMKTLADLTDEGVAATGGPGTPAGARIAEMRDFYRFVAADIDGLLTRWQAYKR
                     QRDREDAR"
     gene            10143..11087
                     /locus_tag="VF995_07185"
     CDS             10143..11087
                     /locus_tag="VF995_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9343382.1"
                     /translation="MTEDAISVSGLHKRFGQTHALDGLDLTVRTGEVHGFLGPNGAGK
                     TTTIRILLGLLRPNGGSAELLGGDPWRDAVALHRRLAYVPGDVTLWPNLTGGEIVDLL
                     GRLRGGLDQKQRDALLERFDLDPTKKARAYSKGNRQKVALVAALAADVELLLLDEPTA
                     GLDPLMEAVFRETVEEAKRAGRTILLSSHILAEVEAICDRVSIIRNGRNVETGTLAEL
                     QHLTRTSVIAETARPIPDLSDLPGVHDLERNGTRIRCQVDTAELNGVLRRLTEAGVLT
                     LTSRPPTLEELFLRHYGDQGDQEDSEPDSGVGKPVEVG"
     gene            11089..>12069
                     /locus_tag="VF995_07190"
     CDS             11089..>12069
                     /locus_tag="VF995_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9343383.1"
                     /translation="MRSLTGTRTLLKLALRRDRVVIPAWVYGVASLVLSTAYGFKKLF
                     PTPAERLQFAAGIAGKATFKALYGPAFDLSTTGGLTAWRITGTAATFAGLMGLLLAVR
                     HTRAEEEAGRTELLGATVVGRHAGLAAALLVVVIANLALAALVTLGLAALGLPLAGSA
                     AMALGLASVGCVFGAVAAVCAQVATTPRGASGAAGALLGLAFILRAAGDNGDGTLSWL
                     SPIGWAQRVQPFATERWAVFALPLLLTAVLVPVAVALAARRDLGAGMLAQRPGRPAAS
                     RWLRSPWGLAWRLQRGSLLGWTVGFAVAGVVFGSVAKDVASLGDQVAKFLQ"
BASE COUNT         1549 a         4335 c         4668 g         1517 t
ORIGIN      
        1 cgttgccgga tcgagggcgg ggcctgcctg agcgtcgccg gggcggccgc cgtgcggggg
       61 cagggcgcca cgctggccat cgccaggggc ggcgcccgcg tcgttgccgt cggaaccatc
      121 ggaaccatcg ggccggttgg ggcgggaacc gccgggcccg gcggggtcag ggtcgtcgtc
      181 gtcgggcccg gcgccgagga tgcggtccag ttcccgctcg tcgaggccgg ggctgtccat
      241 ggggccgcgg cggcggcggt gcggcagcgc gagcagggcg gcgctgcgga cgtcggcgac
      301 ctcgacctgg tcgcggcctg cccaggcggc cagggccatg gcggtcttgg cgcacacgat
      361 gtcgccgcgc aggccgtcga caccgaacgc ggcgcacacc tcggcgatgc gggcgagcag
      421 ctcgtcgggc agctgcacgg cggccaggcg ggcacgggcc gcggcgatcc gcccggtgag
      481 ggcagcgtcc gcaccggcga acgcggcggc gaacgcggcg gggtcggcct cgtaggcgag
      541 gcggcggcgg accacctcga cccggtcggc gacggcggcg ttgccgcgca cctccacggt
      601 gacggcgaag cggtccagca gctgcggccg cagctcgccc tcctccgggt tcatcgtccc
      661 gaccagcagg aagcgggcgg cgtggcgcac gctgacgcct tcgcgctcga cgtagttggt
      721 gccgagcgcg gcggcgtcca ggagcaggtc gaccaggtgg tcgccgagca ggttcacctc
      781 gtcgacgtag aggatgccgc ggtgggcggc ggcgagcagg cccggctcga agacgcgccg
      841 gccctctccc agcgcctgct ccaggtcgag agagccggcc acccggtcgg tgctggcacc
      901 gaccggcagc tcgaccagtg ggaccggccg gcgtttggcg ggcgcgtccg ccgggtgcgg
      961 gccggcgggg caggccgggt cgggcgctgc tgggtcgcag ttgaaggggc agccgccgac
     1021 cacctcgatc tcgggcagca gccgggcgag gccgcggacc gcggtggact tggcggtgcc
     1081 cttctccccc cggaccagca ccccgccgat cgccggggag accgcgttga gcagcagcgc
     1141 cagggtcagg tcgggctgcc cgaccagagc cgagaacggg tacggcgcgg tgccggtgcc
     1201 cagccctcgc cgctcagcct gagcttcagc ttcagccacc acgttcctcc agttcgcctt
     1261 caagttccag gtaggtggcc tggagccggc caattgtctc cgggtcgggg gcgtcccaca
     1321 gcttccggtc ggccgcctcc agcagccgct cggcgatggc gcgcagcgcc cacgggttgg
     1381 cggtgcgcag gaacgcgccg ttctcctggt caaggacgta gcgttcgctg accgcctggt
     1441 acatccagtc ctcgaccacg ccggtggtgg cgtcgtagcc gaacaggtag tcgacggtgg
     1501 cggccagctc gaaggcgccc ttatagccgt ggcggcgcat cgaggcgatc cagcgggggt
     1561 tgaggacccg ggcgcgcacc acccggcgga cctcttcggc gagcgtgcgg gtccgcaccc
     1621 gcgcgggatc ggccgagtcg ccgatgtagg cgtggggcgc gccgccgagc gcgcgcacgg
     1681 cggcgaccat gccgccgtgg tactggaagt agtcgtcgga gtcgagcagg tcgtgctcgt
     1741 cggtgtcgac gttcttggtg gccacgcgga tgcgcgcgaa gttggtccgc atcgcctcca
     1801 gcgccggcgc gccgtccaag ccgcggccgt aggcgtagcc gccccaaacc tcgtagacct
     1861 tgaccaggtc ggcctcggtg cgccagttgc gcgcgtcgat cagcggcagc aggccggcac
     1921 cgtaggcacc cggtttggac ccgaagacgc gggtggtggc gcggcgttcg tcggcgccgg
     1981 cggcgcggtc ggcgcgggcg tgcttggcca ggtagttgac ctcgtcgggc tcgtccaggc
     2041 cggcgaccag ggtgaccgcg tcgtcgagca gggcgaccag gtttgggaag gcgtcgcgga
     2101 agaagccgct gatgcgcacg gtgacgtcga tgcggggccg gccaagctcc ccgggtggga
     2161 ccgcctccag gccggtgacc cggcgcgact cggggttcca gcggggccgg acgccgagca
     2221 gcgcgaggat ctcgccgatg tcgtcgccct gggtgcgcat cgccgaggtg ccccagacca
     2281 cgatcccgac ggtctccggc cagtcgccct gcccgtccgc ctcgatcccg gcctggccct
     2341 ggtcgcggtc cgtgcgccag cggcgcagca ggtcgtcggc gaggcgccgc ccggtggcgt
     2401 aggccagttc cgagggcagg cccttggggt cgaccgagta gaagttgcgc ccggtcggca
     2461 gcacgtcgac ccgcccgcgc gtcggcgccc cggacggtcc tgccgggacg aagcggccgt
     2521 cgagcgcgcg gaccaggttg accagctcgt cgctggtgcg ctccagccgg ggcacgacct
     2581 cgcgggcggc gaaggcgagc acgcgggcca ccgcagccgc cgccgcctcc agctggatcc
     2641 ctgcttcggg gtcagggtgg aggatgcggc tggtgacggc ggcggcttgc tcgggatccc
     2701 agcgggcctc gtccatcgcg gcgagcagcg ccatgctggc ggcctccagc tggtcgagcc
     2761 gctggcggtc gccagccttc agcagcgccg cctcgtccag gccggccgcc gcggcgacgg
     2821 cggcgcgcag ccccggcagg ccggcgctgc ccagccgcag gatcgcggcg agcaggccga
     2881 ggcgctgctc cccggcgggc gcctggccga ggatgtggag gccgtcgcgg atgcgggcgt
     2941 ccttgatctc gcagaggtag ccgtccaggt gcaggacgaa ctcgtcgaag gagtcgtcgc
     3001 ccggcggtgc ctccacgccg aggtcgcggt gcagctcggc ggtgcggacc agctcccaga
     3061 tccggccgcg gacctctgcc agcttggcgg ggtcgagcgc ctgcacctgg gcgtgctcgt
     3121 cgaggagctg ttcgagccgg gcgaggtcgt cgtaggtctc ggcccgctgc atcggcggga
     3181 ccagatggtc gatcaccacc gcgtgggcgc ggcgcttggc ctgggtgccc tcgccggggt
     3241 cgttgacgat gaacgggtag accagcggca gcggcccgag caccgcgtcc ggcgcgcacg
     3301 ccgccgacag gccgaggccc ttgccgggca gccactccag ggtgccgtgc ttgccgaggt
     3361 gcacgatcgc gtcggcgccg aagccgtcct cgtgccgcag ccaccagtag gcggccaggt
     3421 agtggtgcga gggtggcagc tcggggtcgt ggtagatggc gatcgggttc tcgccgaagc
     3481 cgcgtggcgg ctggatcgcc aggaacaggt gctcgctgag gcacagcccc gcgagcacga
     3541 tcgcgtcgcc gtcgacgtac agctcgccgg gcggctcgcc ccagtggcgc accacggccg
     3601 cgcgcagcgg ctcggtgagc gtctggaacc agcgggcgta ggcggccgcg tcgatccggc
     3661 cgctggcgcc ggcgagctgc ccctcggtga ggaactcgct gtcgtggccg ccggcggcga
     3721 tcagccggtg gatcagcgcg tccccgtcgg tggggacctc gccggtgtcg tagccggcgt
     3781 cccgcagcgc cgcgagcagc cgcagcgcgc tggcgggggt gtcgaggccg acggcgttgc
     3841 cgactcgggc gtgcttggtc gggtagttgg agagcatcag cgccacgcgc ttggcggcgt
     3901 tgggggtgtg ccgcagccgc gcgaggcggg tggcgatggc ggcgagcgcg gcggcccgct
     3961 ccgggtcggc gcggtagacc agcgcgccac tgtccgacct ggacgcaccg ctgtcgttgt
     4021 gcgccgctcc cggtgcgctt acctcgccac cgatgggttc cttgaaggag aacggcacgc
     4081 cgatgatgcg gccgtcgaac tcggggatgg cgacctgcat ggcggcgtcc agcggcgaca
     4141 gcccgccgcg ggaggcgagc caggccgcgc gcgtggaggt ggcgcagatc ccctgcacga
     4201 tcggcacgtc gagggcgtcg agcgcgggga cctgccagcc gtccgggtcg gcggcgtcgg
     4261 cggcgtggga accgccggcc gccaggacgg tgacgatcag cgcgtcggcg ctgtgggcgg
     4321 cgagcagctc gcaggcggcc acgcggccgg tggggtcggg ccgcagcgag tagcagaaca
     4381 gcggcagcgg gtttgccccg gcggcctcca gcgcctcgac gagggtgtcg acgaaggcgg
     4441 tgttcccgct catccagtgg gtgcggtaga agaccacccc gacggtcggc cgttccggct
     4501 ggtggcggtc gagcagctcg ggccggtaca gcccggcctc gggcaggacg gccggcggct
     4561 cgaagccgaa gccgccgtac agcaacctgt ccgccacgaa gcggaacagg ttcgccatgt
     4621 tggcgacgcc accgtgacgg aggtattcga aggcctcggc gaccaccccg gccggcgcgg
     4681 tcgaggccgc ggccagctcg gcgtccggct cggcctcacc cccgaaggcg agcagcggca
     4741 tcccggcggc gtggcagtcg gcggcaagct ggtcgaaccc ggccggccag gcacgccggc
     4801 cgccgagcag ccgcaccagc accaccttcg cctccccgag ctcgacggtg gcggggctgg
     4861 cggggtccgc ggtcgggttg gcggcgcgca ccggcggaaa ggcgccgggg agggtggcgc
     4921 gggcgcggcg tgccgccagg atctcggtgt cggcggtggt gaggaacagg atcaccgcgc
     4981 gcctccaacc tgtgggtaac ccgggacggc gccatgctcg atgaggcggt cgagggcggc
     5041 ggtgtcggcg tgctcggcga tcaggtcgcc gagggcgtcg aggcgctcgg cgcggacggc
     5101 ggcgaacggg cgatcgccgg ggatccagtc gaggccgcgc tcggccgcga cccaggtgag
     5161 gaaggcgcgg cggaaggcgt cgccctcgaa gatgccgtgc caggcaagtc cggccaccgt
     5221 gccctggcgg cagccctggt cgccgctggt gctggcgagc accggctcgc cgccgaagcg
     5281 gcggatgcgg ccgtggtgga tctcgtagcc ggccgctggg acgccgccgt acgcgggggc
     5341 ggttccggtc gggttcgcga gcgtcttggt ggcggcgaac acggtctcga cggggagcag
     5401 gccgagcccg gcgacctcgc cggcatcgct ctcgacggcg tcccggtcgg cgatccggcc
     5461 gccgagcatc tggtagccgc cgcagatccc gagcgtcggg aggccgcgcc gtgcccgggt
     5521 ggcgatcgct tggtcgaggc cgcgggcgtg cagccaggcg aggtcgtcga cggtcgcctt
     5581 gctgccggga agcacggcga ggtccgcgct gagcagctcc gccggcgact gggtgaagcg
     5641 gaccaccacg cccggctcgg cggcgagcgc gtcgaagtcg gtgaagttcg acagccagcg
     5701 cagccgcgcg acggccacca ccagcgaatc cgcgccgagc ggcggcagcg caccgtcgcg
     5761 gggcgcctcc agcgccagcg agtcctccac gtcgagccac agcccgcgcc gccacggcag
     5821 caccccgagc accgggcggc cggtgagggc gcggagctgc tccaggccgg gcgcgaggat
     5881 cgcctggtcg ccccggaact tgttgatcag gaacccggcg accagcgcct ggtcggcggc
     5941 gtcgagcagg gcgagggtac cgaacaggct ggcgaacacc ccgccgcggt cgatatcgcc
     6001 gaccaccact accggcaggc ccgccgcgcg tgccagaccc atgttgacca ggtcgccgtc
     6061 gcggaggttg atctcggctg ggctgccggc gccctcgcag acgaccacgt cgaagcgttg
     6121 ccgcaggtcc gccagcgcct ccaggaccac gccgcggagc cgaggcttga gctgctggta
     6181 cgagcgggcg tccacggtgg tgaacggctc ccccatcacc accacctggc tgacgcgctc
     6241 gccggccggc ttgagcagga tggggttcat cgccgcctcg ggcgtggcac cggcggcggc
     6301 ggcctgcgcg acctgcgccc ggccgatctc ggcgccgtcg ggggtgaccg cggagttgag
     6361 cgccatgttc tgtgccttga acggtgcgac cttgagcccg cgccgcgcca gccagcggca
     6421 gatcccagcg acgacgacgc tcttgcccgc gtccgaggcg gtcccggcga ccagcagcgc
     6481 gccgctcacc gggaccgctt ccgctgccgg agcgccgtgg tgagggcggc cagcgccacg
     6541 gcgagtgcca cggtcgcgac gctcacctgg cgggagaggc gcaccgcgcg gtccacgtcg
     6601 tcggggccgg gcgcgggtcc gtcgccgagg gtggggcgct gctcgacctg gtcgccgtag
     6661 cggttgcggc cgcccagccg cacgccgagg gcgccggcga aggcggcctc catgcggccg
     6721 gcgttggggc ttgggtgagc ggtgccgtcg cggcgcagga ctcgccaggc ccgccagcgg
     6781 tcgccgtcgc tggcgccagt ggcggcgagc aggcaggcga gcaccgcgcc gaggcgtgcg
     6841 gccggccagg tgagcaggtc gtcgaggcgc gcggcggccc agccgaagcg gagatagcgg
     6901 gagctgcggt ggccgaccat cgcgtccagg gtgttggcgg cccggtaggc ggccacgccg
     6961 gccgggccgg cgagggcgcc ccacagcagc gggccgacca ccgcgtcggc ggtgttctcc
     7021 gccaccgact cgatcgcggc gcggcacagc tcgacgccgt cgagcctgct cgggtcgcgc
     7081 ccgaccaggg agggggcgag ctggcgggcg cgctccaggt ccccctcccg gaccgcctcg
     7141 ccgagctggc gggcggtgcg ggccagcgag cgcccgccga gtgcggtcca ggtgagcccg
     7201 gcgagcagca gtgcccgcgc cagcggccgc tggcgaaggc gcgcgtcagc cagcgccgcg
     7261 gcggtcgcgg cgccgccgac cagcacgagg gtgtaggcgg tgcccgcggc ccgcgacggg
     7321 cgccagacac gccgctccag cacgaccgcg gcctggccga agccggccac cgggtggccg
     7381 cgccgcgggt cgcccaggaa ctgatcggcg agcgcgcccg ccagcaggcc ggctggagcg
     7441 acgcccctgg tgcgcgccat caggtggccg tccagttgac ccgggcgagc gcccagccac
     7501 gctcgcggcg atgcaggacc gtgagggagg cgggggcgat gtcgatgcgc cagaaggcgg
     7561 cgggcggtgc gccgaggacg agcagcgccg cggcacgtac ccagccgctg tgggtgaccg
     7621 cgacggtggc gccgcgcgcg gctccggtcc tgatcccgcc gccgtcctgg tcctcgtcgc
     7681 cgttgctgtc gctgccgagg atgcgggtgt gcaactggtc gagacaggcg ctgacccggg
     7741 tgagcaggtc gcggcgggac tcgccgccgg gcgggcgcgc gtcggggtcc tcgagccagg
     7801 cggcgaggcg ctccgggtcg cggtcggcga cctcgtcgag ggtgagtccc cgccacgcgc
     7861 ccgcgtcggc gtcggcgagc gcctcttcga cggtggcatc gaggccggcc gcggcggcgg
     7921 tctggcgggc acgcagggcc gggctggtga gcacctggcc cgcccgccgc ggctgcaggc
     7981 agggaacgtg ggtgagggcg cccgcgtcgc gctccccacg cgcgaccagg ggctcgtcgt
     8041 cggggaacgc cgcccgcagg gtcgccgcgg ttgccgcgct gcgcagcagg atcagccggg
     8101 ccacaggctt cagctccccg cgagccgcgg ccgctccacc ggcgccgcgg cgccgtcgcg
     8161 ctcgctgtcg gtggcgctgc cggcttggcg ctggcgctgc tcggcgcggg ctgacagcca
     8221 gccgaagctc acgcccagca gcagccacag cagcgcggca cccgcggccg agacctctcg
     8281 cgcctgccac agcagcgcgg ccggcagggc ggtcgcctca gcggtgtcgg gcagcagcac
     8341 ccacgcaccg gcgaccaccg cgaggtaggc cgtgccgacc agcacctgcc gggtctcgcg
     8401 gcgcatgccg tcgcgggcca gccagcgagc cagcagccag gcgcccacgg cggcgccgac
     8461 tgagaccgcg agcatgatga ggtacagccg ctgccgctcc ggcgcggtcg ccgcgctgcc
     8521 catccccggc gggttggccg ggtacttgag gaacgggacc agccacagcg ccgcgaagca
     8581 cacgccggcg agccgcagcg agcggtccca gccgctgtga gcggccaggc gcgggccaag
     8641 cagggcgtag acgacggtga acagcgcgcc gagggcgatc ccgaacaggc tcaccccgac
     8701 gaccaggccg acccgctggg tgctccgcga gaagacctgc atggtatgcg tcccggcggg
     8761 gacggtctca taatgcagcg cctgctggat gaccggctcg ccgaccagca ggtagaagac
     8821 gccagcgacc aggccggcca gcaggccggc gatcaggccg cggcgcagca gcgaagccac
     8881 gacggcgctc cttgtgacgg ttcctggctc gtttggcgct tgctcggggc cacctcgtgc
     8941 gtgtcagtgg caggggaagc cgagcaggtg ccgcgcgtcg tggaagaact cgtgcaggta
     9001 gttgccgctg gcggcggcct tcccgagggc cggctccagg agttggccct ggtcgtagcc
     9061 gatcgcgaag accagcagcg cgaccagtcc gacggcgacc caggcccacc agggaacggg
     9121 gacgccgagc gggcgggacg ggctggggct ggcgctgggg ccagggatcg ctgccgccgc
     9181 cgatgttggc gaagccattg gctctgaacc tcctcggggt cctgcgcccc ggtcggacgg
     9241 gcggcgacgg ccagtatcct ggctcccgga tcggcgctcg cctccgcctt cccaccctgg
     9301 aggggcagtg gcacggatga agctcgctcc ccggttacag tggcgcgacc gcgccggact
     9361 cgcaccggct tcctggttca cgtcgccaac ggcgccagcg gcaccgtggc cggaacctac
     9421 gtggccgccc agcgcctgtc aagcacgcaa ctccccacat cacgggaggc agcgcctcaa
     9481 cgagcatcct cgcggtcgcg ctggcgcttg taggcctgcc atctggtgag cagaccgtcg
     9541 atgtcggccg cgacgaagcg atagaagtca cgcatctccg cgatccgcgc accggcgggc
     9601 gtgcccggac cgccggtcgc cgccacgccc tcgtcggtga ggtcggcgag cgtcttcatc
     9661 atcccgcgcc ggacgatatt ggcctcgtag aaaggatcgt cgggaaggcg gtaacggtcg
     9721 cggcgagtcc ccgggaccgg ctcgcgggcg agcagcccca actgcatcag gtagcgcacc
     9781 gctccggaga tcgccgcggg gctgacgtcc agccgctccg ccaggtcggc ggcgctgagc
     9841 gagggctcgt ccatgctcat catggtcatc agcacccgtg ccgccatacg ggcgaagccc
     9901 cactcggcga actgcatcgc catgtgctcg acgaagcggc gaaccgcctc ctcgtcgcgg
     9961 gtcctggaag tctggtcagg catgcacggc tcctgcctgt tgcttgtctc gctgtcggcc
    10021 tgcctgtcaa taccacagct ggcgctccat aagtttaaca aactaatcat gatttacaag
    10081 gttatgaatg aaatgtagcc tgctgctacc agactgccct cgctcgatgg aggcgagaca
    10141 tcatgacgga ggacgcgatc agcgtttccg ggctgcacaa gcgcttcggc cagacccacg
    10201 cgctcgacgg gctcgacctc accgtccgaa ctggcgaagt gcacggcttc ctcggtccga
    10261 acggtgccgg gaagaccacc accatccgca tcctgctcgg cctgctgcgc cccaacgggg
    10321 gcagcgccga gctgctcggc ggcgatccct ggcgcgacgc cgtcgccctg caccggcgcc
    10381 tggcctacgt gcccggcgac gtgaccctgt ggcccaacct caccggcggg gagatcgtcg
    10441 acctgctcgg ccggcttcgc ggcggcctcg accaaaaaca gcgcgacgcg ttgctggagc
    10501 gcttcgacct ggaccccacc aagaaggccc gcgcctactc caaaggcaac cggcaaaaag
    10561 tcgcgctggt cgccgcgctc gccgccgacg tcgagctgct gctgctcgac gagccgaccg
    10621 cggggctcga cccgctcatg gaggcggtgt tccgcgagac cgtcgaggag gccaagcggg
    10681 ccggccggac catccttctg tccagtcaca tcctggccga ggtcgaagcc atctgcgacc
    10741 gggtgagcat catccgcaac ggccgcaacg tcgagaccgg caccctcgcc gagctgcagc
    10801 acctcacccg cacctcggtg atcgccgaga ccgcccgccc gatcccggac ctctccgacc
    10861 tcccgggcgt ccacgacctg gagcgcaacg ggaccaggat ccgctgccaa gtggacaccg
    10921 ccgagttgaa cggggtcctg cggcgcctca ccgaagccgg cgtcctcacc ctgaccagcc
    10981 gcccgccgac gctggaggag ctgttcctgc gccactacgg cgaccagggc gaccaggagg
    11041 actccgagcc ggactcgggc gtgggcaagc cggtggaggt gggctgagat gaggagcctt
    11101 acgggcacca gaacgttgct gaagctggcg ctgcgccgcg accgcgtggt gatcccagcg
    11161 tgggtgtatg gggtcgcgtc gctggtcctg tcgaccgcct atggcttcaa gaagctgttc
    11221 ccgaccccgg cggagcgcct gcagttcgcc gccggcatcg ccggcaaggc gaccttcaag
    11281 gcgctgtacg gccccgcgtt cgacctctcc accaccggcg ggctgacggc ctggcgcatc
    11341 accggcactg ccgccacctt cgcggggctg atgggcctgc tgctcgccgt ccgccacacc
    11401 cgcgccgagg aggaggccgg caggaccgag ctgctcggcg cgaccgtcgt cggccgccac
    11461 gccgggctcg ccgcggcgct gctggtggtc gtgatcgcca acctggcgct ggccgcgctg
    11521 gtgacgctcg ggctggccgc gctcggcctg ccgctggcgg gctcggccgc gatggcgctc
    11581 ggcctcgcct cggtcggctg cgtcttcggc gcggtcgcgg ccgtgtgcgc ccaggtcgcc
    11641 accacccccc ggggcgcgag cggcgcggcc ggagcgctgc tcgggctggc ctttatcctg
    11701 cgggcggcgg gcgacaacgg ggacggcacg ctctcgtggc tctcccccat cggatgggcg
    11761 cagcgcgtcc agccgttcgc cactgaacgg tgggcggtgt tcgcgctgcc gctgctgctg
    11821 accgccgtgc tggtgccggt cgcggtggcg ctggccgccc ggcgcgacct cggcgcgggc
    11881 atgctggcgc agcggccagg gcgccctgcc gcctcgcgct ggctgcgcag cccctggggg
    11941 ctcgcgtggc ggctgcagcg gggttcgctg ctcggctgga cggtcgggtt cgccgttgcg
    12001 ggcgtcgtct tcggctcggt ggccaaggac gtcgccagtc tgggcgacca ggtcgccaag
    12061 ttcctccag
//
LOCUS       DASWDC010000220         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_307494, whole genome shotgun sequence.
ACCESSION   DASWDC010000220 DASWDC010000000
VERSION     DASWDC010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307494"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="VF995_07195"
     CDS             <1..116
                     /locus_tag="VF995_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9343384.1"
                     /translation="VETYMSYLRRKVDRFDPPLLRTVRGVGYTLRLPKPRA"
     gene            132..>1557
                     /locus_tag="VF995_07200"
     CDS             132..>1557
                     /locus_tag="VF995_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9343385.1"
                     /translation="MSPHARLHRDLTVSLRARLISRLLLLLAAGLIAAAVCTSLLLRS
                     FLIARLDRQLGSVATATESYVASWRGPGLPPFDPKSDFGVAMSQLGVLPTFAELRGPD
                     NRVLLPLSSKSHPTPPLPAVLPVGPPTAANPSGQRFFTVAPRLGGREERPPYRVVVAR
                     LPDGRGTFAMGLPLTDVMETVGRLIRTEALVGAVVLLALALLARRAVRRGLRPLVEIG
                     ETARAIGAGDLTRRVEPATERTEVGRLGLALNAMLGQLEAAFREREASERRLRRFVAD
                     ASHELRTPLTSIRGYAELFRRGASARPEDLATAMRRIEEEAGRMGVLVDDLLLLARLD
                     QGRPLERGQVDLAALAGEAVADARVVEPDRPLRLERDGPVVVGGDADRLRQVLANLLA
                     NLRQHTPAGTGATVRVAREEPYGVLEVDDQGPGLTPEQRERVFERFWRAEPWRPRTRT
                     RRDGGGERERTPAGGAGLGLAIVAA"
BASE COUNT          167 a          594 c          596 g          200 t
ORIGIN      
        1 tggtcgagac ctacatgagc tacctgcggc gcaaggtcga ccgcttcgac ccgccgctgc
       61 tgcggacggt gcgcggcgtc ggctacaccc tgcggctgcc gaagccgcgg gcgtaggcgg
      121 ggaggccggc ggtgtcgccg cacgcgagac tccacaggga cctgacggtc tcgctgcgtg
      181 cccggctgat cagccggctg ctgctgctgc tcgcggcggg cctgatcgct gcggcggtgt
      241 gcacctcgct gctgctgcgc tcgttcctga tcgcacggct cgaccgccag ctcggctcgg
      301 tcgcgaccgc caccgagagc tacgtcgcga gctggcgggg accaggactg ccgccgttcg
      361 accccaagag cgacttcggc gtggcgatgt cgcagctcgg cgtgctgcca acgttcgcgg
      421 agctccgcgg cccggacaat cgggtcctcc tgcccctttc gtccaaaagc caccccacgc
      481 cgcccctgcc ggcggtgctg ccggtcggtc cgccgaccgc ggccaacccg tccggccagc
      541 gcttcttcac cgtcgccccc cgcttgggcg ggcgcgagga gcgccccccc taccgggtgg
      601 tggtggcccg gctgccggac ggccgcggca ccttcgcgat ggggctgccg ctgaccgacg
      661 tgatggagac cgtcggccgc ctgatccgca ccgaggcgct ggtcggcgcg gtggtgctgc
      721 tcgcgctcgc gctgctggcg cggcgcgcgg tgcggcgcgg gctgcggccg ctggtggaga
      781 tcggcgagac cgcccgcgcg atcggtgccg gcgacctcac ccgccgggtc gagccggcca
      841 ccgagcggac cgaggtcggg cggctcggcc tcgcgctgaa cgccatgctc gggcagctgg
      901 aggctgcctt tcgggaacgg gaggcgtccg agcggcggct gcgccgcttc gtcgccgacg
      961 cctcccacga gctgcgcacg cccctcacct cgatccgcgg ctacgcggag ctgttccgcc
     1021 gtggcgccag cgcccgcccc gaggacctcg cgacggcgat gcgccgtatc gaagaggagg
     1081 ccgggcgcat gggcgtgctg gtcgacgacc tgttgctgct cgcccgcctc gaccagggcc
     1141 gcccgctgga gcgcggccag gtggacctgg ccgcgctggc cggcgaggcg gtcgccgacg
     1201 cccgcgtggt cgagccggac cggccgctgc ggctggagcg ggacggcccg gtggtggtcg
     1261 gtggcgacgc cgaccgcctc cgccaggtgc tcgccaacct gctcgccaac ctgcgccagc
     1321 acacccccgc cggcaccggc gcgaccgtcc gggtggcccg cgaggaacca tacggcgtgc
     1381 tggaggtcga cgaccagggg ccgggcctca cccccgagca gcgcgagcgt gtcttcgagc
     1441 gcttctggcg cgccgagccg tggcgccccc ggacccggac gcggcgcgac ggcggcgggg
     1501 agagggagcg cacgcccgcc ggcggggctg ggctcggcct ggcgatcgtc gcggcgg
//
LOCUS       DASWDC010000221         7490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1390654, whole genome shotgun sequence.
ACCESSION   DASWDC010000221 DASWDC010000000
VERSION     DASWDC010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390654"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VF995_07205"
     CDS             complement(<1..555)
                     /locus_tag="VF995_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9343386.1"
                     /translation="MSQPIEPAGGGGARRGGVSRRVGAIAESATVAVDAKAKALKAAG
                     EDVIGFGAGEPDFPTPPHIVAAAQAACRVPRYQHYTPTAGLPELREAIAAKTARDSGY
                     QVAPSQVLVTNGGKQAIYEAFATLLDPGDEVLLPAPYWVTYPEAIALAGGVAVLVETT
                     ETTGFRAHLEQLEAARTPRTKALLF"
     gene            660..1859
                     /locus_tag="VF995_07210"
     CDS             660..1859
                     /locus_tag="VF995_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9343387.1"
                     /translation="MPSITLPDHLRPRDGRFGSGPSKVRPEAIAALAQAAPTYLGTSH
                     RQAGVRSMVASLKAGLVELFRPPDGYEIALGNGGTSAFWDVAAYSLIERQSQHLVFGE
                     FSAKFAAAAAAPHLAAPQLVESPAGTHPTPRPDAAVDLYALTHNETSTGVAMPVRRPS
                     VQASEAMLSTGGRRSRPVPAGAGAGAGAGALVAVDATSGAGGLPVELAEADAYYFAPQ
                     KCFASDGGLWVAILSPAAIERAEAIAASGRYVPATLSLTAALENSRLDQTTNTPALAT
                     LFLFVRQLDWLNQHGGLDWAVKRCESSASVLYGWAEASDYAIPFVADPAQRSSTVVTI
                     DLDAAVVSADAVAKVLRANGIVDTESYRKLGRNQLRIAAFPAIDPEDLETLTRAIDWV
                     VGRLRQR"
     gene            complement(1928..2161)
                     /locus_tag="VF995_07215"
     CDS             complement(1928..2161)
                     /locus_tag="VF995_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9343388.1"
                     /translation="MVTAYILIQTEVGKAAQVAKEIAEIKGVQQAEDVTGPYDVIVRA
                     EARNVDDLGKLVVARVQAVDGITRTLTCPVVHL"
     gene            2412..3134
                     /locus_tag="VF995_07220"
     CDS             2412..3134
                     /locus_tag="VF995_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX9343389.1"
                     /translation="MLWLAFPLEWGRVPAVTERHFIDRVDAGERLADALAPHAPQPEV
                     ILAIPRGGVVVGAAVARRLGMPLDVVVPRKVGAPGNPELAVGAVAEGVEVIDHQGVRR
                     LGLDPAAVQAAARAESEEVARRTAAYRAGRPPLDLTGRTVALVDDGVATGWTCVAAAR
                     WCRSAGAELVILAVPVGPAGLWRRLGPVVDEVVVLETPEPYLAVGQVYARFPQVSDDE
                     VLRCLEGLRKEGEHQGGRQPPR"
     gene            3206..4756
                     /locus_tag="VF995_07225"
     CDS             3206..4756
                     /locus_tag="VF995_07225"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX9343390.1"
                     /translation="MTSTTPSDLGVVGKSISFRPTEAELRAYTDAMPQARQTRYGNVN
                     VQTKVVARSKSSTYIVTDKPEEHSDQTISREEGARIARLQDEYIAGRDMVVVDGFIGH
                     AGPYRTPARLIIEQANANIAGMQRFLYYDPVEGDAPHTPGITIVYTPNLTMPGYSNDR
                     VIAVWLEEGVSRVLNSDYFGESKKGGLRMWNKRVYEGGGLAMHAGCKVIPSSQGPKTM
                     LIVGLSGTGKTTTTFTKQNGSQAVQDDFVGLFPGGVVVSTEDGCFAKTFALDPTDEPA
                     IYGAVTKPDAYLENVSQHGEELDFFDTSYTQNGRAVFNMRSIDNFPADQAPPASFLLI
                     LNRNENIIPGVAKLNIEQAAGYFMLGETQGTSAGGADEAGKALRVPGTNPFFPLRHEQ
                     QGNRLAELLSTHPLQVYLLNTGRVGGPDDDDRSKKVRIPHSSAIVAAIAEGSIIWDED
                     PDFGYQVASEVPGIDDPELLQPRRLYQRQGREGEYRQYVDRLKAERARYLAGFPGLDE
                     AIVKAVTT"
     gene            4813..5874
                     /gene="thiL"
                     /locus_tag="VF995_07230"
     CDS             4813..5874
                     /gene="thiL"
                     /locus_tag="VF995_07230"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX9343391.1"
                     /translation="MIARLTERAGAGGVASVAGVEVGVGDDAAVLVPAPGARLVATTD
                     VLVEGHDFRHDLSEPADWGWKAVAANLSDLAAMGARGRWLLLALTAPAATPVATLEQL
                     YAGVGEACQAFGVALVGGDVSDGPALSLAVTALGEAPAGQVVTRSGAQPGDRLVVSGP
                     LGAAAAGLALLELALELEPRRGRDPAAEPAEPAEPGSRRGDRARELLARFPGLAAAQR
                     RPHPDLGAGPRLAQAGASAMLDVSDGLAGDALHLAEASRVGVEIRDGDVPLAPGVAEA
                     ASLFDRDPVELALGGGEDFVLAAALPDPAGDVTGTIACGTFTSGATHGPGPGPGQGVR
                     RTAAGTVPLGGLAYQHFRR"
     gene            5976..6344
                     /locus_tag="VF995_07235"
     CDS             5976..6344
                     /locus_tag="VF995_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343392.1"
                     /translation="MKDRIDMLRRPGPPSLRERRRWARSIEKLVADANRRILQRAYWP
                     LANTTAVHRATPALLDVATALRQQDLLVSRDLLGELRAFLTDGGASPLYGNHPIAALW
                     AAEELRRRFAPVTAPLRHAA"
     gene            6468..6932
                     /locus_tag="VF995_07240"
     CDS             6468..6932
                     /locus_tag="VF995_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9343393.1"
                     /translation="MSVKQPTTRDAATFQSVSPATGEVVGTFPIDGPAQVAAALDRAR
                     TAAAWWAGLGFDGRKRRLLDFKRLLVRRGEELCDLIHRENGKPHDDAFLELLIAVEHL
                     DWAARKAAKILRPERVAPTLLARNVAASIHYQPLGVVAVIGPWNQPLSVSTR"
     gene            7310..>7490
                     /locus_tag="VF995_07245"
     CDS             7310..>7490
                     /locus_tag="VF995_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343394.1"
                     /translation="MDTDQSDVSSYLDHLPAIFRQEPFVGRFLRAFETILSGTDAEPP
                     GLETTISHLADYLDPT"
BASE COUNT         1066 a         2848 c         2553 g         1023 t
ORIGIN      
        1 gaacagcagc gccttggtcc tcggggtgcg ggccgcctcc agctgctcca ggtgggcgcg
       61 aaagccggtc gtctcagtgg tctcgaccag caccgccacg ccgccggcca gcgcgatcgc
      121 ctccgggtag gtgacccagt acggcgccgg cagcagcact tcgtcgcccg ggtcgagcag
      181 ggtcgcgaag gcctcgtaga tcgcctgctt gccgccgttg gtgaccagca cctggctggg
      241 cgcgacctgg tagccggagt cgcgagcggt cttggccgcg atcgcctcgc gcagctccgg
      301 cagcccggcg gtgggggtgt agtgctggta gcgcggtacc cggcaggcgg cctgcgccgc
      361 cgccacgatg tgcggcgggg tggggaagtc cggctcgccc gcaccgaagc cgatgacgtc
      421 ctcgcccgcc gccttcagcg ccttggcctt ggcgtcgacc gccacggtgg ccgactcggc
      481 gatggcgccg acgcgccgcg agacccctcc gcgccgcgcg cctccacctc ctgctggttc
      541 aatgggctgc gacatcgatc cgacggcctc ctcagatgat tctgggacgg gcactcgaca
      601 ggacacaacg gacatgacag gaacaaggaa accgcatcgt ggcacatgac gctcgttcca
      661 tcccctcaat taccttgcca gaccacctcc gcccccgcga cgggcgcttc ggctccggtc
      721 cctccaaggt ccggcccgag gcgatcgccg cgctggccca ggccgccccg acctacctcg
      781 gcaccagcca ccggcaggcc ggggtccgct cgatggtggc cagtctgaag gccgggctcg
      841 ttgagctgtt ccggcccccg gacggctacg agatcgcgct cggcaacggc ggcacctcag
      901 cgttctggga cgtggccgcg tactcgctga tcgagcgcca gagccagcac ctggtgttcg
      961 gcgagttctc cgccaagttc gccgccgccg ccgcggcgcc gcacctggcc gccccgcagc
     1021 tcgtcgagag ccccgccggc acccacccga cgccgcggcc cgacgccgcc gtggacctgt
     1081 acgccctgac tcacaacgag acctcgacgg gggtggccat gccggtccgc cggccgagcg
     1141 tgcaggcgag cgaagcaatg ctgtccacgg gaggccggcg gagccggccc gtcccggccg
     1201 gtgccggtgc cggtgccggt gccggtgcgc tggtcgcggt ggacgccacc tccggcgcgg
     1261 gcggcctgcc ggtggagctg gccgaggccg acgcctacta cttcgcgccg cagaagtgct
     1321 tcgcctccga cggcgggttg tgggtcgcga tcctgtcgcc cgccgcgatc gagcgggccg
     1381 aggcgatcgc cgcctcgggc cgctacgtcc cagcgaccct cagcctcacc gccgccctcg
     1441 agaactcgcg gctggaccag accaccaaca cccccgcgct ggccaccctg ttcctgttcg
     1501 tgcggcaact cgactggctg aaccagcacg gcggcctcga ctgggcggtc aagcgctgcg
     1561 agagctccgc ctcggtcctg tacggctggg ctgaggcgag cgactacgcc atcccgttcg
     1621 tggccgaccc ggcgcagcgc agcagcaccg tcgtcaccat cgacctggac gccgcggtgg
     1681 tctcggccga cgccgtcgcc aaggtcctgc gcgccaacgg catcgtcgac actgagtcct
     1741 accgcaagct cggccgtaac cagctgcgca tcgccgcctt cccggcgatc gacccggagg
     1801 atctggagac ccttacccgc gcgatcgact gggtggtggg ccgcctgcgg caacgctgac
     1861 cgccgcttgg tgggcgggct gagccggcaa ccacacggac taggacgaac aggccatgcc
     1921 gatcggccta caggtggacc accgggcagg tgagcgtccg ggtgatgccg tcgacggcct
     1981 ggaccctggc gaccaccagc ttgccgaggt cgtcgacgtt gcgggcctca gcacggacga
     2041 tcacgtcgta gggaccggtc acgtcctcgg cctgctggac gcctttgatc tccgcgatct
     2101 ccttggcgac ctgggccgcc ttgccaactt cggtctggat caggatgtac gccgtcacca
     2161 tcagcaccga cctccttgag ccgccgccgc cggcggcacc agccacccgc gggacatgtc
     2221 ggtctgcccg aaccgccgcg cgtcgccggg cctggtgggg ttgtccggcg ccctccggtc
     2281 gaaaggtggg cgcatggtac acgcggttcc aaggcatgcc aaggattgat tagttacctg
     2341 gcgtagttac tcggccgtca ccagccgaac ccggttggcg cgtgccctta ctgggaacta
     2401 ccgtgaccgt tatgctctgg ttggccttcc ctctcgaatg ggggcgcgtc cccgcggtca
     2461 cggaacggca cttcatcgat cgggtcgacg ccggcgagcg actggccgac gccttggctc
     2521 cgcacgcccc ccagcccgag gtcatcctgg cgatcccgcg cggtggggtg gtcgtcggcg
     2581 cggcggtggc acgtcgcctc gggatgccgc tggacgtggt ggtcccgcgc aaggtcggcg
     2641 cgccgggcaa cccggagctg gcggtcgggg cggttgccga gggtgtcgag gtgatcgacc
     2701 accagggggt gcgccgcctc ggcctggacc cggcggcggt ccaggcggcc gctcgggccg
     2761 agtccgagga ggtcgcccgg cgcaccgcgg cctaccgcgc cgggcggccg ccgctcgacc
     2821 tgacgggccg caccgtggcg ctggtcgacg acggcgtggc gaccggctgg acctgcgtgg
     2881 cggcggcgcg ctggtgccgc agcgccgggg cggagctggt gatcctggca gtcccggtcg
     2941 gccccgccgg gctgtggcgg cgcctcggcc ccgtggtcga cgaggtggtg gtgctggaga
     3001 ccccggagcc ctacctcgcg gtcggccagg tttacgcccg cttcccccag gtcagcgacg
     3061 atgaggtgct gcgctgcctg gaggggctcc gcaaggaggg cgagcaccaa ggggggcggc
     3121 agccgccacg gtagacttct gtgttggagt ttccggctgg gcgacgcgcc caaccgtagc
     3181 agcgaggcgc ttggaggcaa ctggtatgac gagcaccacc ccctctgacc tgggtgtcgt
     3241 cggcaagtcg atctcgtttc gcccaaccga agcggagttg cgcgcataca ccgacgcgat
     3301 gccgcaggcc cgccagaccc gctacggcaa cgtcaacgtc cagaccaagg tggtggcccg
     3361 ctccaagtcg tcgacctaca tcgtcaccga caagcccgag gagcactccg accagaccat
     3421 cagccgcgaa gagggcgccc gcatcgcgcg actgcaggac gagtacatcg ccggccgcga
     3481 catggtggtg gtcgacggct tcatcggcca cgccggcccc taccgcaccc ccgcgcggct
     3541 gatcatcgag caggccaacg ccaacatcgc cggcatgcag cgcttcctgt actacgaccc
     3601 ggtcgagggc gacgctcccc acacccccgg catcaccatc gtctacacgc cgaacctcac
     3661 catgccgggc tactcgaacg accgggtgat cgccgtctgg ctggaggagg gcgtctcgcg
     3721 ggtgctcaac tccgactact tcggcgagtc gaaaaagggc ggcctgcgga tgtggaacaa
     3781 gcgggtctac gagggcggcg ggctcgccat gcacgccggc tgcaaggtca tcccgagctc
     3841 ccagggcccg aagacaatgc tgatcgtcgg cctgtcgggc accggcaaga ccaccaccac
     3901 cttcaccaag cagaacggct cccaggcggt ccaggacgac ttcgtcgggc tgtttcccgg
     3961 cggcgtggtg gtctcgaccg aggacggctg cttcgccaag accttcgcgc tcgaccccac
     4021 cgacgagccg gccatctacg gggcggtcac caagcccgac gcctacctgg agaacgtcag
     4081 ccagcacggc gaggaactgg acttcttcga cacctcctac acccagaacg gccgcgccgt
     4141 cttcaacatg cgctcgatcg acaacttccc cgccgaccag gccccgcccg cgtcgttcct
     4201 gctgatcctc aaccgcaacg agaacatcat ccccggcgtc gccaagctca acatcgagca
     4261 ggccgccggc tacttcatgc tcggcgagac ccagggcacc tccgccggcg gtgccgacga
     4321 ggccggcaag gccctgcgcg tccccggcac caacccgttc ttcccgctgc gccacgagca
     4381 gcagggcaac cgcctcgccg agctgctctc gacccacccg ctccaggtct acctgctgaa
     4441 caccggccgg gtcggcggcc ccgacgacga cgaccgctcc aagaaggtcc gcatcccgca
     4501 ctcctcggcg atcgtggctg ccatcgccga gggcagcatc atctgggacg aggaccccga
     4561 cttcggctac caggtcgcca gcgaggtgcc cggcatcgac gatcccgagc tgctgcagcc
     4621 gcgccgcctc taccagcgcc agggccgcga gggcgagtac cgccagtacg tcgatcgtct
     4681 gaaggccgag cgcgccaggt acctcgccgg cttccccggc ctcgacgagg cgatcgtcaa
     4741 ggcggtcacc acctgagcga ccgggacgcc gcaccgtcgc cggagtcgct ccgctccctc
     4801 ggcgagttcg gcctgatcgc ccggctcacc gaacgggcgg gagccggcgg ggttgcgagc
     4861 gtcgcgggcg tggaggtcgg cgtcggcgac gacgcggcag tgcttgtccc ggcgccgggc
     4921 gcccgcctgg tcgccaccac cgacgtgctc gtcgagggcc acgacttccg ccatgacctg
     4981 tccgagccgg ccgactgggg ctggaaggcg gtcgccgcga acctctccga cctggcggcc
     5041 atgggcgccc gcgggcgctg gctgctgctg gccctgaccg ccccggcggc gacgccggtg
     5101 gcgacgctgg aacagctcta cgctggcgtc ggggaggcct gccaggcctt cggggtggcg
     5161 ctggtgggcg gcgacgtctc cgacggcccg gcgctgtcgc tggctgtcac cgcgctgggt
     5221 gaggcccccg ctgggcaggt ggtgacccgc tccggcgccc agcccggcga ccgcctggtc
     5281 gtcagcgggc cgctcggcgc cgccgccgcc ggcctggccc tgctggagct ggcgctggag
     5341 ctggagccgc gccgcgggcg cgaccccgcc gcagaacccg cggagcccgc agaaccggga
     5401 tcgcggcgag gagaccgggc gcgggagctg ctggcccgct tcccgggcct cgccgccgcg
     5461 cagcgccgcc cccacccgga cctcggcgcc gggccgcggc tcgcccaggc cggagcgagc
     5521 gcgatgctgg acgtgtccga cgggctggct ggcgacgccc tgcacctcgc cgaggcgtcg
     5581 agggttgggg tcgagatccg cgacggcgac gtgccgctgg cacccggcgt ggcggaggcg
     5641 gcctcgctgt tcgaccgcga cccggtggag ctggcgctcg gcgggggaga ggacttcgtg
     5701 ctggccgccg ccctgcccga ccccgccggc gacgtcaccg gcacgatcgc atgcggcacc
     5761 ttcaccagcg gcgccaccca cggccccggt cctggtcccg ggcagggggt gcgccgcacc
     5821 gccgccggga ccgtcccgct cggcggcctc gcctaccagc atttccgccg ctgattcccc
     5881 ggccgcattc gcggttcgag cggttttgcg ggctcctttc ggggatagac atgccgtatg
     5941 atggactcga ttcagtcacc cttgtcatct cccttatgaa ggacaggatc gacatgctcc
     6001 gccgccccgg accgccgagc ctgcgggagc gcaggcgctg ggcccgctcg atcgagaagc
     6061 tggtggccga cgccaaccgg cggatcctgc agcgcgccta ctggccgctt gccaacacca
     6121 ccgcggtcca ccgcgccacc ccggcgctgc tcgacgtcgc caccgcactg cgccagcagg
     6181 acctgctggt ctcccgcgac ctgctgggcg agctgcgggc gttcctcacc gacggcggcg
     6241 cctccccgct gtacggcaac cacccgattg ccgccctctg ggccgccgag gagctgcgcc
     6301 gccgtttcgc gcccgtcacg gccccgctgc gccacgccgc ctgacccgcc gtcggcttcg
     6361 cggcatcgtt ccctgccccc gccgagggga caaggccata cttgactcgt cggtaaccta
     6421 gccgtgccgg gcaggagcag gacgccattt cgggaaaggc ggccacgatg tcggtcaagc
     6481 agcccaccac ccgcgacgcc gctaccttcc agtccgtcag cccagcgacc ggcgaggtcg
     6541 tcgggacctt cccaatcgac ggccccgcgc aggtcgccgc cgcgctcgac cgcgcccgta
     6601 cggcggcggc ctggtgggcc gggctcgggt tcgacggccg caagcgccgg ctgctggact
     6661 tcaagcggct gttggtgcgc cgcggcgagg aactctgcga cctcatccac cgggagaacg
     6721 gcaagccgca cgacgacgcc ttcctggagc tgctgatcgc cgtcgagcac ctcgactggg
     6781 cggcgcgcaa ggcggccaag atcctgcgtc ccgagcgggt cgcgcccacc ctgctggcgc
     6841 gcaacgtcgc ggcgtccatc cactaccagc cgctcggcgt ggtggcggtc atcggcccgt
     6901 ggaaccaacc cctatccgta agtacccgat aggccggctc cgatccacgg ggacctgacc
     6961 cggccagatc cgggccggcc agatccgacc cggccagatc cgacccggcc agacccgggc
     7021 cgcgcggaac cgggctgtgc gaccccaacc caccaacggc tggcccacgg gaacccgaca
     7081 tcggaagccc aaatccacga tcagccgacc cgccgggacc cggtcccaga gaacccaacc
     7141 caccagcgtc cgacccgtcc tccccgatcg gcagcacccc cgtaatcgag ggcggccgtg
     7201 tggtggtggg cgtcgagctg gacgcccacg agctggtgcg gctccagacg accgcgctgg
     7261 tggcctatga cgtccgtggc aagccgtacc ggtagccggc aggagcgaca tggacaccga
     7321 ccaatccgac gtgagcagct acctcgacca tctcccggcg atcttccggc aggaaccgtt
     7381 cgtcggccgc ttcctgcggg ccttcgagac gatcttgagc ggcaccgacg cggagccgcc
     7441 cggcctggaa accaccatca gccacctcgc cgactatctc gacccaacca
//
LOCUS       DASWDC010000222        11315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1309800, whole genome shotgun sequence.
ACCESSION   DASWDC010000222 DASWDC010000000
VERSION     DASWDC010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309800"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1044)
                     /locus_tag="VF995_07250"
     CDS             complement(115..1044)
                     /locus_tag="VF995_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343395.1"
                     /translation="MTQPGPASRALELYVGPIAMELTFWRAELAVLQEELNQREADLA
                     DFERHLSLFETQYRRTLSDRYALLDDLADQIERARSQIGTNGQSGRTAGSDVDEQAIR
                     ENSGWFESGGPRGDGDDGDGSGQSQNWAWAWGEREPEPAKRPVVGETAKRLFRKLARL
                     IHPDLALNHAERERRTDLMVRANYAYEQGDEPTLQRLLDDWRRSPESVVGTRPEADLE
                     RTLRRIAQVHERIDGIGKHIEELEASAMGWLRHRVEKAAGEGWDLLSHMVKELDRQIT
                     EAQLELERLQAPPDHDHAWDRDRDRGNPQTAWA"
     gene            complement(1264..2049)
                     /locus_tag="VF995_07255"
     CDS             complement(1264..2049)
                     /locus_tag="VF995_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX9343396.1"
                     /translation="MTDTRKTLSYLIDMDGVLVHDDQPLPGAEQFIQRLRETSRSFLV
                     LTNNSIYTPRDLSARLARGGIHVPPESLWTSALATAKFLDEQRPNGTAFVVGEAGLTT
                     ALHEAGYVIVDADPDYVVLGETRTYSFEAITRAIRLVEGGARFIATNPDATGPSPGGP
                     LPATGSVAALITRATGKAPYFIGKPNPLMMRSALRTLEAHSETTTMIGDRMDTDIIAG
                     LEAGLETILVLTGVSSRDDADRFPYRPSEVVESLADIELENEL"
     gene            2612..3658
                     /gene="add"
                     /locus_tag="VF995_07260"
     CDS             2612..3658
                     /gene="add"
                     /locus_tag="VF995_07260"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX9343397.1"
                     /translation="MDVATALDRLPKVELHCHVEGTMRPKTVIDLASRNGIPMPAADP
                     TELYRYSSLDTFLEVFWLVQSTLATREDWARLAYESVIDGAAHGLVYRETFFTPARHL
                     AAGQKLGEILAGLAEGLEAAEAETGVRVLLICDLDRAYGGAAGRELVERLVELRRAAT
                     PAPGTERVLGIGLDSTELGVDPADFAEAFALAAKAGLRRTSHQGEETPPSHIRAGLDV
                     LGCERIDHGLSSLQDPELTRRLAAERIPLTVCPNSNVLIANAVPRLALHPYRRMRDAG
                     LLATLNTDDPALTDLDLGQEYRSVMTAFGYTFRDMVQIALDGVEATWLDDSDKLALSR
                     HVEDQAAKLAATLS"
     gene            complement(3935..4924)
                     /locus_tag="VF995_07265"
     CDS             complement(3935..4924)
                     /locus_tag="VF995_07265"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX9343398.1"
                     /translation="MGVISYFQAIVIGLLQGVTELFPVSSLGHSVLLPALLGWSGILK
                     DQAAQESPFLAFLVGLHVATALALLWFFREDWARIVAGLLRSLRTRQIETADERLGWL
                     LVVATIPAGLVGVIFEHELRTLFAKPLAAAIFLTINGGILLAGERLRRASGIGDLERR
                     LDPRRLEAIARGERQHERLVDAEDGMALLQPPRSIPATPRRARALSTLSFPEATLIGA
                     AQILAFFAGISRSGVTMVTGLSRGLDHEDAARFAFLLATPIILAAGVYKLPDFLGPNG
                     NGVRGQILVGSLVAAGAAYVSVRFLIRWFETRTLTPFAIYCLVAGILCIIRFS"
     gene            complement(4915..5547)
                     /locus_tag="VF995_07270"
     CDS             complement(4915..5547)
                     /locus_tag="VF995_07270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9343399.1"
                     /translation="MPKGPARALFNGPRSGTRVALTFHGSGDPALADQLLGVVEHAGA
                     KVTVFAVGTWLQQNPQMAARILGAGHELDNHTLTHPVLGRLGATQVAQQIRGCQSVLR
                     KLGAGPGGGHYFRPSGMTTPTPLVLAQAGAAGYPLVVSFDVDPRDYTDPGATAVAERV
                     AAGVRPGSIVSLHTGHAGTVTAMSDILATLHNRGLEPVPLRRLLVGEPSA"
     gene            complement(5730..6932)
                     /locus_tag="VF995_07275"
     CDS             complement(5730..6932)
                     /locus_tag="VF995_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX9343400.1"
                     /translation="MNPTRSLRRVAALLLALPLAGAACSGGHAQLTKADPPSVNRSLG
                     TTTTPPGSPLAVASGLLPGMPPPLDPHDLYAADRPDQLSPATRGFRPLVYVPNSDSAT
                     VDVIDPASFKIVEHFNVGRLPQHVTPSWDLKTLWVDNDQGNSLTPINPRTGRPGTPVP
                     VDDPYNLYFTPDGKFAIVVAERLRRLDFRDPHSMRLRRSVPVPCMGVDHMDFSANGRY
                     AIVSCEFSGMMLKVDIARQRVAGTLLLRAGGMPQDVKLSPDGRVFYVADMASGGVWLV
                     DGDRLKAIGFIHTGVGAHGLYVSRDSRLLYISNRGEGTISVLSFATRKVVRLWRLPGG
                     GSPDMGGVSADGKVLWLSGRYNGEVYAIDTSSGRLLARIRVGSGPHGLCVYPQPGRYS
                     LGHTGVFR"
     gene            7241..10693
                     /locus_tag="VF995_07280"
     CDS             7241..10693
                     /locus_tag="VF995_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX9343401.1"
                     /translation="MEEARALAAQVTGDWAATALALDRLTARLGGVEQLLRGTPGLRS
                     LLDDPGRRQSLDRLATELDGWVERLEARVDAQAATLPADALEPVNAALVGVKDTLWTL
                     RRDRLATAVAVDALAGRDSSDAAVAAFASIEATLAALDRIEVRGRDSAGVHVLVTGHG
                     LDLNDPEIAAQLAARADPLFTSGAARTPRGHLAFVYKAASEVGELGDNGRRLRAAIAA
                     DELLHRAVTAGGAQALVLTHTRWASVGLINEANAHPVNSEERDRGGLPYAVAALNGDV
                     DNYTELIGRHQLHIPAEITTDAKVIPVLLARRVAAKKVPVEEAFRRTVAEFEGSTAVA
                     ANAAAAPGKLLLALRGSGQGLFVGLADDLFMVASEPYGLVAECDRYLRLDGETPAVPG
                     DLASRGQMVVLDRAEAGTLAGIHRFDYQGNELPVAEAELRRSEITTRDIDRGAFPHYL
                     LKEISEAPSSFRKTLRGRLRDLDGQLSVDLGPEALPEELHRRLDAGLLCRVFVIGQGT
                     AAVAGQAVAALFGDVLRDLGWRISAPLATELSGFGLEPDMRDTLVVAISQSGTTTDTN
                     RTVDLLRARGALVVAVVNRRDSDLTHKADGVLYTSDGRDVEMSVASTKAFYAQVAAGA
                     LLACALAEAAGIGDRGERHQLLEGLHALPGAMTRVLGERERIRELAQRHAPQRRHWAV
                     VGNGRNRVAAEEIRIKLSELCYKSIACDATEDKKHIDLSSEPLTLVCAAGLSGSAADD
                     VAKEVAIFRAHAGAPIVIASEGETRFDLAAGVIPVPEAHPSLAFVLSTVAGHLFGYEA
                     ALALDAQALPLRQARAALERAVAEAGPGNAATALDRLAPSLAPAAAQFRERLRAGFYD
                     GTLNAGTASTLVTLLHYAEGRAPLDTWQGQGDVDAIASPGALLDQLSVALTGAIDELT
                     RPVDAIKHQAKTVTVGISRSDESLVTVPLVRQLLAAGAARDRLAYGTLRTVAALDPAV
                     ARVTGYTRYHVELDAAGSGGTIAIADRGGVSRDIPSRTERDQRLRGTKQLVAAQRQVL
                     AARGRSDGRTFVLVPEAKDGHTTGLVLLHVEFHEQLPAERTRRVLDGYRGRLTALRSA
                     VTETEPAFDESLLGTLPVERLLTEPVEVLADHWTSRADPEPGPEGPEGPEGPEGPEGS
                     PAEA"
     gene            complement(10707..11309)
                     /locus_tag="VF995_07285"
                     /pseudo
     CDS             complement(10707..11309)
                     /locus_tag="VF995_07285"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT         1596 a         4009 c         4123 g         1587 t
ORIGIN      
        1 tccctatcgc tgctacggag ctgcaggtcg cgcgataggg accccctccg ggcctacggt
       61 ggatccatgc ggcgcaggct cgcagctcac ccctaggaaa ggtgtcgagt ggggtcaggc
      121 ccaggcggtc tgcgggttgc cccggtcccg gtctcggtcc caggcgtggt cgtggtccgg
      181 gggggcctgg aggcgctcca gctccagctg ggcctcggtg atctggcggt caagctcctt
      241 gaccatgtgg gagagcagat cccagccctc gccggcggct ttctccacgc ggtggcggag
      301 ccaccccatg gcagaggcct ccagctcctc gatgtgcttg ccgatgccgt cgatccgctc
      361 gtgcacctgg gcgatgcgcc gcagggtccg ctccaggtcg gcctcgggcc tggtgccgac
      421 gacgctctcg gggctgcgtc gccagtcgtc gagcagccgc tgcagggtgg gctcgtcgcc
      481 ctgctcgtag gcgtagttgg cacggaccat caggtcggtg cgccgctctc gctcggcatg
      541 gttgagcgcc aggtccgggt ggatcagccg ggcgagcttg cggaacagcc gcttggcagt
      601 ctcgccgacc accggccgct tggccggctc aggttcgcgc tccccccagg cccaggccca
      661 gttctggctc tggcccgagc cgtcgccgtc gtcgccgtcg ccgcgggggc cgccggattc
      721 gaaccagccc gagttctcgc ggatcgcctg ctcatcgacg tcgctgcccg cggtgcgtcc
      781 ggactgcccg ttggtgccga tctgcgacct ggcccgctcg atctggtcgg cgaggtcgtc
      841 gagcagcgcg tagcggtcgc tgagcgtgcg ccggtattgg gtctcgaaga ggctcaggtg
      901 gcgctcgaag tcggccaggt cggcctcccg ctggttcagc tcctcttgca gcaccgccag
      961 ttcggcccgc cagaaggtga gctccatggc gatcggaccg acgtagagct cgagggcgcg
     1021 cgaggctggg cccggttgcg tcatcgcgat cgggctgcca ggagtggggg agagcacaac
     1081 gcaggcatcc gcatgccgct gcgatcctcc gtggcgaaga tcgttgctcg ctccttgcat
     1141 cgggccgcgc cgttccccgg ggccggaccg gtcacggcgg attgcctcat tccaggaggg
     1201 tgacgttacc ctgaccgcgg gtctccctgc tagcgccgct ggtgagcggc gatggagggc
     1261 gttctagagc tcgttctcga gctcgatgtc ggcgagggac tcgacgactt cgctcgggcg
     1321 gtaggggaag cggtcggcgt cgtcgcggga ggagaccccg gtgaggacca ggatggtctc
     1381 gaggcctgct tccaggccgg cgatgatgtc ggtgtccatg cgatcgccga tcatggtggt
     1441 cgtctccgag tgcgcctcca gggtccgcag ggctgatcgc atcatcagcg ggtttggctt
     1501 accgatgaag tagggcgcct tgccggtcgc gcgggtgatc agggcggcga ccgagccggt
     1561 ggcgggcagc gggccgccag gggaggggcc ggtggcgtcg gggtttgtgg cgatgaagcg
     1621 ggcaccgccc tcgaccaggc ggatcgcgcg ggtgatcgcc tcgaagctgt aagtgcgggt
     1681 ctcgccgagg acgacgtagt cggggtcggc gtcgacgatc acgtagccgg cctcgtgcag
     1741 cgcggtggtg aggccggcct cgccgaccac gaaagcggtg ccgttcgggc gctgctcgtc
     1801 gaggaacttc gcggtcgcca gcgctgaggt ccacagcgac tcgggcggca cgtggatccc
     1861 gccgcgggcc agccgggccg acaggtcgcg gggggtgtag atcgagttgt tggtcagcac
     1921 caggaagctc cggctggtct cccgcaggcg ctggatgaac tgctcggcgc cgggcagggg
     1981 ctgatcgtca tggacaagga ccccgtccat gtcgatcagg tagctcaggg tcttcctggt
     2041 gtcagtcatc atgatcaaga tcgggtggtt cccggcgacg gtgaagcggc gttgcgaggg
     2101 atttccataa ttccagctcc tcaccgcttc gtgcagctgc cgaacagcag cgaaggcgcc
     2161 ggacgatcgt ggcaaggtgg aaagtcacaa cttactgccc ggtaatgctt tacaggcggc
     2221 tgggggaggc tcaccataga gaagtccagt gcctgcagtc cttatcggtg gcgggtccac
     2281 cgggaaaggg ggcggttggc atggaggttc caccggacat ataggggcgg ctcacctagg
     2341 tgggtcgcca cccgctaacg ttccgtcggc cgacggacac gcgaactctg gataaccttg
     2401 cggcggccgt gacacagttc cggtccgact gcagaggccc gagacgccgg ccgacggaac
     2461 gcttttttgg gtcccccagc ccttctccgc cgcctacggc gcttcgccta cggcgcttcg
     2521 cctacggcgc ttcgccaacg gtgtgcccgg ttcgagtgtg agaatggcgt acgtccaacg
     2581 gcgggcgtag ggagatggga ggatcacgaa cgtggacgtc gcaaccgcgc tggaccggct
     2641 gccaaaggtc gagctgcact gccatgtcga ggggacgatg cggccgaaga ccgtgatcga
     2701 cctcgccagc aggaacggca tcccgatgcc cgccgccgat ccgaccgagc tgtaccggta
     2761 ctccagcctc gataccttcc tggaggtctt ctggctggtc cagtcgaccc tggcgacgcg
     2821 ggaggactgg gcgcggctcg cctacgagag cgtcatcgac ggcgccgccc acggcctcgt
     2881 ctaccgcgag accttcttca cccccgcccg ccacctcgcc gccggccaga agctcggcga
     2941 gatcctcgcc gggctcgccg agggcctgga ggccgccgag gctgagaccg gcgtccgggt
     3001 gctgctgatc tgcgacctcg accgcgccta cggcggcgcc gccggccgcg agctggtcga
     3061 gcggctggtc gagctgcgcc gcgccgccac ccccgccccc gggaccgagc gggtgctcgg
     3121 catcggcctc gactcgaccg agctgggcgt cgaccccgcc gacttcgccg aggcgttcgc
     3181 cctcgccgcc aaggcaggcc tgcgccgtac ctcccaccag ggcgaggaga caccgccgag
     3241 ccacatccgc gccggcctcg acgtgctcgg ctgcgagcgg atcgaccacg gcctgtcgag
     3301 cctccaggat cccgagctga cccgccgcct cgccgccgag cggatccccc tgaccgtgtg
     3361 ccccaactcc aacgtgctga tcgccaacgc cgtgccgcgc ctggcgctgc acccctaccg
     3421 gcgcatgcgc gacgccgggc tgctcgccac cctcaacacc gacgaccccg ccctcaccga
     3481 cctggacctc ggccaggagt accgctcagt gatgacggca ttcggctata ccttccggga
     3541 catggtccag atcgccctcg acggcgtcga agccacctgg ctggacgaca gcgacaagct
     3601 cgccctctcc cgccacgtcg aagaccaggc cgccaaactc gccgccaccc tcagctagac
     3661 ttccccgtca tgcgctccgc ccgccgccgg atcatcccgc gcgctctgcc aaccctggct
     3721 gccgccaccc tcgtcgccct cgccggcgcc ctcgccctcg ccctccaggc atggcagggc
     3781 gacgccctcg ccgcccatcg caccgccagc aataccgccg tccacccaaa ggcgccgcgg
     3841 cggcatcatc ccccgaccac cgacgatctc gaggggtgga ccgcaagccg gaaccgcgag
     3901 caccagcgat ccccccgacc accctcgaca cctttcagga gaaacgaatg atgcagagga
     3961 tcccggcgac caggcagtag atcgcgaacg gcgtgagcgt tcgggtctcg aaccagcgga
     4021 tcaggaagcg caccgagacg taggcggcgc cggcggcgac caggctgccg accaggatct
     4081 ggccgcgcac gccgttgccg ttggggccca ggaagtcggg cagcttgtag acgccggcgg
     4141 ccaggatgat cggggtggcc agcaggaagg cgaagcgggc ggcgtcctcg tggtcgaggc
     4201 cgcgggacag cccggtcacc atggtcacac ccgagcggct gatgccggcg aagaaggcga
     4261 ggatctgcgc cgcgccgatc agcgtcgcct cggggaacga gagggtggag agcgcgcggg
     4321 cacggcgcgg ggtcgccggg atcgagcgcg gcggctgcag cagggccatg ccgtcctcgg
     4381 cgtcgaccag gcgctcatgc tggcgctcgc cgcgggcgat cgcctccagg cggcgcgggt
     4441 cgaggcgccg ctccaggtcg ccgatgccgc tggcacggcg cagccgctcg ccggccagca
     4501 ggatgccgcc gttgatcgtc aggaagatcg ccgccgccag cggcttggcg aacagggtgc
     4561 gcagctcgtg ctcgaagatc accccgacca gcccggcggg gatggtggca acgaccagca
     4621 gccagccgag ccgctcgtca gcggtctcga tctggcgggt ccgcagcgag cgcagcagcc
     4681 cggcgacgat ccgtgcccag tcctcccgga agaaccacag cagcgccagc gccgtggcca
     4741 cgtgcaaccc gaccaggaag gccaggaagg gcgactcctg cgcggcctgg tccttgagga
     4801 tgccgctcca gccgagcagc gcgggcagca gcaccgagtg tcccaggctg gagacgggga
     4861 acagctcggt gaccccttgg agcaggccga tgacgatggc ttggaagtag ctgatcacgc
     4921 cgatggttct ccgaccagca ggcggcgcag cggaaccggt tcaaggccgc ggttgtgcag
     4981 ggtggccagg atatcgctca tcgcggtcac agtcccggcg tggccggtgt ggaggctgac
     5041 gatcgacccg ggccgcaccc cggcggcgac ccgttcggcc acggcggtcg cgcccgggtc
     5101 ggtgtagtcc ctcgggtcca cgtcgaagga gaccaccagc gggtagccgg cggcgcccgc
     5161 ttgcgccagc accagcggcg tcggcgtggt catccccgag ggccggaagt agtggccgcc
     5221 gccgggtccg gcgccgagct tgcgcagcac cgactggcag ccgcggatct gctgcgcgac
     5281 ctgggtcgcg ccgaggcggc cgaggaccgg gtgggtcagg gtgtggttgt ccagctcgtg
     5341 gcccgcaccg aggatgcggg cggccatctg ggggttctgc tgcagccagg tgccgaccgc
     5401 gaacacggtg accttcgccc cggcgtgctc gacgacgccg agcagctggt ccgcgagggc
     5461 cgggtcgccc gaaccgtgga aggtgagcgc cacccgcgtt ccgctgcgag gcccgttgaa
     5521 cagcgcccgc gcgggtccct tcggcacggt ggggtgggcg ttcgagcccg tcccgcttcc
     5581 ctgggggcgt ccccccgagg tcgggctctc cgtcgaggcg ggggggctgc tcgcgttgtc
     5641 cacaggcgtt gcctggtggc tgcaggacgc gagggtcgcg gcggcggccg cggagaggaa
     5701 ggctcgtcgt gacgagcggg gtttcgccat cagcggaaca ctcctgtatg gccgagcgaa
     5761 tagcggccgg gctgggggta gacgcacaac ccgtgcgggc ctgatccgac ccggatgcgg
     5821 gcgagcagcc ggccgctgct ggtgtcgatc gcgtacacct cgccgttata cctccccgac
     5881 agccagagca ccttcccgtc ggccgacacc ccgcccatgt ccgggctgcc gccgccgggc
     5941 aggcgccaga ggcggaccac cttgcgggtg gcgaacgaca gcaccgagat ggtgccctcg
     6001 ccccggttgg agatgtacag caggcgtgag tcgcggctca cgtacaggcc gtgggcgccc
     6061 acgccggtgt ggatgaagcc gatcgccttc aggcggtcgc cgtccaccag ccagacgccg
     6121 cccgaggcca tgtcggccac gtagaacacg cgtccgtcgg gcgacagctt gacgtcctgc
     6181 ggcatcccgc cggcgcgcag cagcagcgtc ccggcgactc gctggcgggc gatgtccacc
     6241 ttcagcatca tgccgctgaa ctcgcagctc acgatcgcgt agcggccgtt ggcgctgaag
     6301 tccatgtggt cgacgcccat gcacggcacc ggcaccgagc ggcgcagccg catgctgtgg
     6361 gggtcgcgga agtcgagccg ccgaagccgc tcggcgacca cgatcgcgaa cttcccgtcg
     6421 ggggtgaagt agaggttgta cggatcgtcg accgggaccg gcgtgcccgg cctgccggtg
     6481 cgcgggttga tcggggtgag gctgttgccc tggtcgttgt cgacccacag ggtcttcagg
     6541 tcccacgacg gggtgacgtg ctgggggagg cgcccgacgt tgaagtgctc gacgatcttg
     6601 aagctggcgg ggtcgatcac gtcgaccgtg gcgctgtcgg agttgggcac gtagaccagc
     6661 ggccggaagc cgcgtgtcgc cgggctcagc tgatctggcc ggtcggcggc gtagaggtcg
     6721 tgcgggtcca gcggcggcgg catccccggc aggagcccgg aggcgacggc cagcggggac
     6781 ccgggcggcg tcgtggtggt gccgaggctg cggttgacgg atggagggtc cgccttggtg
     6841 agttgcgcgt ggcctccgct gcaggccgct cccgcgagcg gcagggcgag aaggagggcg
     6901 gcgacgcgcc tgagggaacg cgttgggttc ataacctccg aactcaatct gactcgggat
     6961 gtggggagca tgagatcaca ccgcttccac ctcggtggat cgcgcaaaca ggacaggtgg
     7021 cgtttggtgc cgcatcgatc ttctcatcga gtcgcgagac gtggccgggt cggtctcggt
     7081 acgctcttgg cggcgaatcg cgcttgcacg acaggcacag ggcgatcagg cggaagccgg
     7141 cgccggctcg gagggggaat cgttcatgtg tggaatcatc gccgtcgccc ggcggccctc
     7201 ctcacacgcg ccccccggcg cgagaggcgt cctggccaga atggaagagg cgcgagcgct
     7261 ggccgcgcag gtcaccggcg actgggcggc gaccgccctg gcgctggacc ggctcaccgc
     7321 ccggctcggc ggggtggagc agctgctgcg cggcacgccg gggctgcgca gcctgctgga
     7381 cgatcccggc cggcgccagt ccctcgaccg cctcgccacc gagctggacg gctgggtcga
     7441 gcggctggag gcgcgggtcg acgcccaggc tgccaccttg cccgcggacg ccctcgaacc
     7501 ggtcaacgcc gccctggtcg gcgtcaagga caccctctgg acgctgcggc gcgaccgcct
     7561 cgccaccgcc gtcgcggtgg acgccctggc aggccgtgac tccagcgacg cggcggtggc
     7621 ggcgttcgcc tcgatcgagg ccacgctggc cgccctcgac cggatcgaag tgcgcggccg
     7681 cgactccgcc ggcgtccacg tgctggtcac cggccacggc ctcgacctga acgaccccga
     7741 gatcgccgcc cagctggccg cgcgggccga cccgctgttc accagcggcg ccgcccgcac
     7801 gccgcgtggg cacctcgcct tcgtctacaa ggcggccagc gaggtcggcg agctgggcga
     7861 caacggccgc cggctgcgcg ccgccatcgc cgccgacgag ctgctgcacc gcgcggtgac
     7921 cgctggcggc gcgcaggcgc tggtgctcac ccacacccgc tgggccagcg tgggcctgat
     7981 caacgaggcc aacgcccacc cggtcaactc cgaggagcgc gaccgcggcg gcctcccgta
     8041 cgccgtggcc gccctcaacg gcgacgtgga caactacacc gagctgatcg gtcgccacca
     8101 gctccacatc ccggccgaga tcaccaccga cgcgaaggtc atcccggtcc tgctggcccg
     8161 gcgcgtggca gccaagaagg tcccggtcga ggaggccttc cgccgcaccg tcgccgagtt
     8221 cgaaggctcg accgcggtcg ccgccaacgc ggcggcagcg ccgggcaagc tgctgctggc
     8281 gctgcgcggc agcggccagg ggttgttcgt tgggctggcc gacgacctct tcatggtggc
     8341 cagcgagccc tacgggctgg tggccgaatg cgaccgctac ctgcgcctgg acggcgagac
     8401 gccggcggtc cccggcgacc tcgcgagccg cggccagatg gtggtgctcg accgcgccga
     8461 ggccggcacc ctggccggca tccaccgctt cgactaccag ggcaacgagc tgcccgtcgc
     8521 cgaagccgag ctgcgccgct cagagatcac cacccgcgac attgaccgtg gtgccttccc
     8581 ccactacctg ctcaaggaga tctccgaggc gcccagctcg ttccgcaaga ccctgcgggg
     8641 ccgtctccgc gacctggacg gccagctcag cgtggacctc gggccggagg cgctgcccga
     8701 ggagctgcac cgccgcctcg acgccgggct gctgtgccgg gtgttcgtga tcggccaggg
     8761 caccgccgcg gtcgccggtc aggcggtcgc cgccctgttc ggtgatgtgc tgcgcgacct
     8821 cggctggagg atcagcgcgc cgctggccac cgagctgtcc gggttcggcc tggagccgga
     8881 catgcgcgac accctggtgg tggcgatcag ccagtcgggg accaccaccg acaccaaccg
     8941 caccgtcgac ctgctgcggg cgcgtggcgc gctggtggtc gcggtcgtca accgccgcga
     9001 ctcagacctg acccacaagg ccgacggcgt cctctacacc tccgacggac gcgacgtgga
     9061 gatgagcgtc gcctccacca aggcgttcta cgcgcaggtc gccgccgggg cgctgctggc
     9121 ctgcgcgctc gccgaggcgg ccgggatcgg cgaccgtggc gagcggcacc agctgctgga
     9181 gggactccac gccctgcccg gtgccatgac ccgagtcctg ggtgagcgcg agcgcatccg
     9241 cgagctggcc cagcgccacg cgccgcagcg gcgccactgg gcggtggtcg gcaacgggcg
     9301 caaccgcgtc gccgccgaag agatccgcat caagctgtcg gagctgtgct acaagtcgat
     9361 cgcctgcgac gcgaccgagg acaagaagca catcgacctg tcgtcggagc cgctcacgct
     9421 ggtgtgcgcc gccgggctca gcgggtcggc cgccgacgac gtcgccaagg aggtggcgat
     9481 cttccgggcc cacgcgggag ccccgatcgt catcgccagc gagggcgaga cccgcttcga
     9541 cctggccgcc ggggtgatcc cggtgccgga ggcgcacccc tcgctcgcct tcgtgctctc
     9601 gaccgtcgcc gggcacctgt tcggttatga agctgccctg gcgctcgacg cgcaggcgct
     9661 gccgctgcgg caggcccgcg cggcgctgga gcgcgctgtc gccgaagccg ggcccggcaa
     9721 cgcggcaacc gcgctggacc ggctcgcccc gtcgctcgcc ccggcggcgg cgcagttccg
     9781 cgagcggctt cgcgccggct tctacgacgg cacgctcaac gccggcaccg ccagcacgct
     9841 cgtcaccctg ctccactacg ccgaggggcg cgcgccgctg gatacctggc aggggcaggg
     9901 ggacgtggac gcgatcgcca gtcccggcgc gctgctggat cagctgagcg tcgccctcac
     9961 cggcgcgatc gacgagctga ccaggccggt cgacgccatc aagcaccagg ccaagaccgt
    10021 cactgtgggg atctcccgct ccgacgagtc gctggtcacg gtgccgctgg tgcgccagct
    10081 gctggccgcc ggcgcggccc gcgaccggct cgcctacggc accctgcgga ccgtcgcggc
    10141 gctggacccg gcggtggcgc gggtgaccgg ctacacccgc taccacgtcg agctggacgc
    10201 cgccggcagc ggcggcacga tcgcgatcgc cgaccgcggc ggcgtctcaa gggacatccc
    10261 ctcgcgcacc gagcgcgacc agcgcctgcg cggcaccaag cagctggtgg cggcgcagcg
    10321 ccaggtgctc gccgcccggg gccgcagcga cggccgcacc ttcgtgctgg tccccgaggc
    10381 caaagacggc cacaccaccg gcctggtgct gctgcacgtc gagttccacg agcagctgcc
    10441 agccgagcgc acgcgccgcg tgctggacgg ctaccgtggc cggctgaccg ctctgcgcag
    10501 cgcggtcacc gagaccgagc cggccttcga cgagtccctg ctcggcacgc tgccggtgga
    10561 gcgcctgctc accgagccgg tcgaggtcct ggccgaccac tggacgtcgc gggccgatcc
    10621 ggagccgggg cctgaggggc ctgaggggcc tgaggggcct gaggggcctg aggggtcgcc
    10681 agcagaggca tgacgacgtc gtcgaggacc gagacgacga actcgtcggg gaccggagga
    10741 ccttcggcca ggcagcgctg cccaagcatg gcggggccga cgtcggcgac cagctggctg
    10801 gcggcgccgg agcggatctc gccgcgggcg gcggcgcgct gcagcacggc gagccacatg
    10861 cgcttctgcg gctgcttgac ccgttcctgg aacaaacggg cgaaggcgtc gttgcgttcg
    10921 atctctgtca tgaggttctg gaacgcgcag cccaccgggg agttcatcag cgccgccatc
    10981 tggcgcagga ggaggagcag gtcgtcgcgc acgccgccct ggtcgggcgg gcactcgctg
    11041 gacggcagca tgtggttgat cgcgtcggtg atcaggtcgt ccttgtgcgg ccagcggcgg
    11101 tagagggcgg ccttgccggt gtgggcgcag gcggccactc gttccatggt gaggccggcg
    11161 tagccgaagg tccgcagcag gtcgaggacg gcgtcgtaga tcgccttctc gagggtgacg
    11221 ccccggcggc ggtgcagcgc gggctcgcat ggctgctcgg ccgcctgctc agcacctgat
    11281 gcgggcagcg gctcgggcgc tgtgaccacc ccggg
//
LOCUS       DASWDC010000223         7233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1641976, whole genome shotgun sequence.
ACCESSION   DASWDC010000223 DASWDC010000000
VERSION     DASWDC010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641976"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(413..655)
                     /locus_tag="VF995_07290"
     CDS             complement(413..655)
                     /locus_tag="VF995_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018681481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEX9343402.1"
                     /translation="MEDQAVFNRIEELSEEEHELLRRAGSGGLSQEEHARLNDLQVQL
                     DRCWDLMRQRRARRDAGLEPDDAQARDAGTVEDYWQ"
     gene            798..2786
                     /locus_tag="VF995_07295"
     CDS             798..2786
                     /locus_tag="VF995_07295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9343403.1"
                     /translation="MQVLKPSSSSAKSTGEPTAFWRRLLFRLRPPRLRPPRLPRLRPQ
                     RPDLDLRARLDLDLERLRQRWRAPVRLPRVALPWERLALGLLLAEVAVVVAQARDGFS
                     KPFWYDEQWRAYHMSLTGGAFWQQYKQANAPIAGGWILLEKGAALLFGNVELALRLPV
                     ILSFVLAVVLTWRVGRRLLGELAGLLAAGALAVNGPLFQFVPELKPYEVEAMATVAVL
                     LLWLWAAEPGRDLWHRLAAYTGIGLLSVLATAVVFVIVPLLALDLAGALPVPRWRWRS
                     PLELARLRLRLRLRRPPMDGIASLARALSRRLLPGAVAGGIALAHLHWFVLRQSRQLG
                     ATYWRPQFVPRGSAGQAWHFASTQLAGWIPEVVTGPLRQSAAIGWLLCLALLAGTAVA
                     ILVERRLLVLPVTLFGALALQYVAAARWQWPFGFVRVNLFLVPLVYLLAAAGIARSLA
                     LLGRFARSLPSDGDTNGDATGDGGSWTWRRVLRDADAVVLPLALALVVVIAGWGVVPD
                     GLKQIQATSEHTTQPGYGDGIRTLVQRARELQLPGSLVVVSGAMGTKGWAYYMWSYDG
                     WAPDLRDQPPIGPDRTLLMTAPELPGMEALLAAHKDAPQVAVLYMRGTGHDLNGSIDG
                     PLLRAGYRRTLMAGSVYYTGHLDIWVRRTAPSLRVTRP"
     gene            2863..3474
                     /locus_tag="VF995_07300"
     CDS             2863..3474
                     /locus_tag="VF995_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX9343404.1"
                     /translation="MEKTRLEAFSDGVFAVAITLLVLDLRVPQAVRPGGLGRALNDLW
                     PNYASYVVSFFIIGIIWVNHHGMFGHIRRVDRPLLFLNLLLLLFVALIPFPTALLASY
                     VRSGGANSHLAAAVFSGAMFGMSASFALNWLWILRDGRLLSEHVDLAAARATIPRFAV
                     FGIVAYLVLTGLSFVSAPLTLALHFLLAVFYIFNQLPVAQVAR"
     gene            3510..4904
                     /locus_tag="VF995_07305"
     CDS             3510..4904
                     /locus_tag="VF995_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HEX9343405.1"
                     /translation="MPAAATRPTAVRDHCLAPSRVIDAPLQGASGKYGRLFPEVPPLH
                     ADETALLRLGVDDGPCDDGAAVVDSGDDGGVDAGWPLFGQFIAHDITADRSALLADVD
                     DRGQVVNFRTPRANLECLYGDGPTGSPFLYDRDDGAKLLVGGNDLGEPGDLPRNAQGI
                     ALVGDPRNDVHLFVSQLHLAMLKVHNRLVDRLREDGEAEAELFDAARRAVAWHYQWII
                     VNEFLPLVAGEALVDELLLEGPRWYRPEGQPQIPLEFADAAYRYGHSQIRRGYRLRAD
                     GPALPLFPDLLGFQPVPASRRVDWSQLFQFPDRPVPQAAKRIDGRLARCLIQLPMAIT
                     GAVEVAAFRSLATRDLQRGQSYGLPSGEALARHLGAEPLSADEVGLAAHGWEGETPLW
                     YYLLREAAARGGGDRLGPIGGRIVAEVLLGIIDHDPESYRAVERDWQPTLPAARPGRF
                     GIADLLLAADAADH"
     gene            complement(5006..5932)
                     /locus_tag="VF995_07310"
     CDS             complement(5006..5932)
                     /locus_tag="VF995_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343406.1"
                     /translation="MAADGSVQSGQQERRPGDVAPAAADQPRPATATGRRNDAGSLET
                     LAPRGSPRDTIYQELTNGGGNWRSADLLVATGTRLGMPWGRGDSPDPESQGSAFEVAA
                     TLYLGAGRHDAAFSALKGAKLAYEDLAVQAGKAEAGSAAEAHAQDLGLGVYALRAQVG
                     LHRYLQRERRGSDGMRLLAEAAADLSVADRPPGDSRPPIFEVELAKAMRARVESRHGS
                     TDARRQASAPSLRRARAALEASEAADSLAAAMLALRHGRLELEWALHGLQAGRMQDAT
                     THFHAALDRSTRSAWQASPRPPTRDWRTRMRR"
     gene            complement(6033..6449)
                     /locus_tag="VF995_07315"
     CDS             complement(6033..6449)
                     /locus_tag="VF995_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX9343407.1"
                     /translation="MGRPGRRAPRPLHRQPGADRDGPRPLRAGALLALGSDNGASSSY
                     GPNFSLEDAKAFHAAALHAAIDDARAKADAAASLIGATVTGVASIDEQGGGQPPILTL
                     TTRAPASSGGTNPVPVSPGTQEVSASVTVVFSYSRP"
     gene            complement(6368..6901)
                     /locus_tag="VF995_07320"
                     /pseudo
     CDS             complement(6368..6901)
                     /locus_tag="VF995_07320"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
     gene            complement(7010..>7233)
                     /locus_tag="VF995_07325"
     CDS             complement(7010..>7233)
                     /locus_tag="VF995_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343408.1"
                     /translation="QDRAGGSPESARLYYTVALAGFDHLHQAGFAARAHHGLAVSYRA
                     LGAQRRASEHEQRAAELCGALCRGDGLTG"
BASE COUNT          812 a         2633 c         2679 g         1109 t
ORIGIN      
        1 agctcctcgg gctccagctc ctcgggctcc agctcctcgg gctcgaccac gccgagctcg
       61 accacgccgc cgacgtcggc gcccagcacc acggctccgc cgagcacctt gccgaccacg
      121 accggcgcgc catcggccac cacctgacct gacctgacct gacctgagcg gcgcgccgca
      181 ccccgagtca ccctccccgg ggtggtcgca ttggtggttc ggctggttcg tcggttaagc
      241 tcggcgcttc gattcgcacg gcacaacgca tggcaggagt ggacgtggac gtgggcatgc
      301 tgctgaacaa catcctgatg accgggctcg tgtacctcct ggcctggcgg atcgccaagc
      361 gcgtcacccg ccggccgccg gatgacagcc gctgacccgc ggttcgccgc gcctactgcc
      421 agtagtcctc caccgtgccg gcgtcgcggg cctgggcgtc gtcgggttcg aggccggcgt
      481 cgcgccgcgc ccggcgctgg cgcatcaggt cccagcagcg atcgagctgg acctgcaggt
      541 cgttgaggcg ggcgtgctct tcctggctga ggccgccgga gcccgcacgg cggagcaatt
      601 cgtgctcttc ctcgctcagt tcctcaatgc ggttgaatac tgcctgatct tccatcgtgc
      661 ccaccctccg tcgcgcgccg ccgagtgtcg ggacgacggt acaaccaccg cgcacccgtc
      721 cctgtaccct gccggaaatg aagcttctgt tacggattca ctgacttctc attcattcgt
      781 gccagccttg gacgagtatg caggtcctca agccgtcatc atcatcggcc aagtccaccg
      841 gggagcccac cgccttctgg cggcggctcc tgttccggct gcgcccgccg cggctgcgcc
      901 cgccgcggct gccgcggctc agaccgcagc gccccgacct tgatctgcgt gcgcgtctgg
      961 acctggactt ggagcgcctg cggcagcgct ggcgcgcacc ggtgcggctg ccgcgggtgg
     1021 cactgccgtg ggagcggctg gcgctcgggc tgctgctggc cgaggtggcg gtggtcgtcg
     1081 cgcaggcgcg ggacgggttc tcgaagccgt tctggtacga cgagcagtgg cgcgcgtacc
     1141 acatgtcgct caccggcggc gccttctggc agcagtacaa gcaggccaac gcgccgatcg
     1201 cgggcggctg gatcctgctg gagaagggcg cggcgctgct gttcggcaat gtggagctgg
     1261 ccctgcggct gccggtgatc ctcagcttcg tgctcgcggt ggtgctcacc tggcgggtcg
     1321 ggcggcgcct gctgggtgag ctcgccggcc tgctggcggc gggcgcgctg gcggtcaacg
     1381 gcccgctgtt ccagttcgtg ccggagctga agccgtacga ggtcgaggcg atggcgaccg
     1441 tggcggtgct gctcctgtgg ctgtgggcgg ccgagccggg ccgcgacctc tggcaccgcc
     1501 tggcggccta caccggcatc gggctgctgt cggtgctggc gaccgcggtg gtgttcgtga
     1561 tcgtcccgct gctcgccctc gacctggccg gggcgctccc ggtcccgcgg tggcggtggc
     1621 gctcgccgct ggagctggca cggctccgcc tccgcctccg cctccgccgg cctcccatgg
     1681 acggcatcgc ttcgctcgcc cgcgccctca gccggcgtct gctgcccggc gcggtcgcgg
     1741 gcgggatcgc cctcgcgcat ctgcactggt tcgtgctgcg gcagtcgcgc cagttgggcg
     1801 cgacctactg gcgcccgcag ttcgtcccgc gcggcagcgc cgggcaggcc tggcacttcg
     1861 ccagcacgca gctggcgggg tggatcccgg aggtggtgac cggcccgctg cgccaatccg
     1921 ccgcgatcgg ctggctgctg tgcctggcgc tgctggccgg gacggccgtg gcgatcctgg
     1981 tggagcgccg gctgctggtg ctgccggtga cgctcttcgg tgcgctcgcc ctgcagtacg
     2041 tcgccgccgc acgctggcag tggccgttcg ggttcgtgcg cgtcaacctc ttcctggtcc
     2101 cgctggtcta cctgctggcc gccgctggga tcgcccgctc gctggcgctg ctgggacgct
     2161 tcgcccgctc gctgcccagc gacggcgaca ccaacggcga cgccaccggc gacggcggca
     2221 gctggacctg gcggcgggtg ctgcgcgacg ccgacgcggt ggtgctcccg ctggcgctcg
     2281 cgctggtcgt ggtgatcgcc gggtgggggg tggtgccgga cggcctcaag cagatccagg
     2341 ccaccagcga gcacaccacc cagccgggct acggcgacgg catccgcaca ctggtgcagc
     2401 gggcccgcga gctgcagctg ccgggcagcc tcgtggtggt cagcggcgcc atgggcacca
     2461 aggggtgggc gtactacatg tggtcctacg acggctgggc gccggacctg cgcgaccagc
     2521 cgccgatcgg cccggaccgc accctgctga tgaccgcccc cgagctgccc ggcatggagg
     2581 cgttgctggc cgcccacaag gatgccccgc aggtggcggt gctctacatg cgcggcaccg
     2641 gccacgacct gaacggctcg atcgacgggc cgctgctgcg ggccggctac cgccgcaccc
     2701 tgatggccgg ctcggtctac tacaccgggc acctggacat ctgggtgcgg cggacggcgc
     2761 ccagcctgcg cgtcacccgg ccgtgaccac gaagggaacg cgacagcggc gtccaggatt
     2821 cgtcgcttgc gacttgcaag tgacccggat atggtcaacg gcgtggagaa gacacggttg
     2881 gaggcgttca gcgacggcgt cttcgccgtc gcgatcacct tgctggtgct tgacctgcgc
     2941 gtgccgcaag cggttcgccc tggcggcctc ggccgggcgc tcaacgacct gtggccgaac
     3001 tacgccagct atgtggtgag cttcttcatc atcgggatca tctgggtcaa ccaccacggc
     3061 atgttcggtc atatccggcg ggtggaccgg ccgctgctgt tcctgaacct gctgctgttg
     3121 ctgtttgtcg cgctgatccc cttccccacc gcgctgctgg cctcctacgt ccgttccggg
     3181 ggggccaact cccatctggc cgccgctgtc ttcagcggcg cgatgttcgg catgtcggcc
     3241 agcttcgccc tcaactggct gtggatcctg cgcgacggcc ggctgctcag cgagcacgtc
     3301 gacctggcgg cggcacgcgc gaccatcccg cgcttcgccg tcttcgggat cgtggcctac
     3361 ctggtgctga ccggcctgtc gttcgtcagc gcgccgctca ccctcgcgct gcatttcctg
     3421 ctcgccgtct tctacatctt caaccagctg cccgtcgccc aggtcgcccg ctgagtgccg
     3481 cggagcgctg ggtaaggagg aggatcgccg tgcccgccgc cgcaacgcgt ccgaccgccg
     3541 tccgggacca ctgcctggcg ccctcacgcg tcatcgacgc gcccctgcag ggcgccagcg
     3601 gcaagtacgg gcgcctgttc cccgaggtgc cgccgctgca cgccgatgag accgcgctgc
     3661 tgcgcctcgg cgtcgacgac ggcccctgcg acgacggcgc cgcggtggtg gacagcggcg
     3721 acgacggggg cgttgacgcc ggctggccgc tgttcggcca gttcatcgcc cacgacatca
     3781 ccgccgaccg ctccgcgctg ctggccgacg tggacgaccg cggccaggtg gtgaacttcc
     3841 gcactccgcg cgccaacctc gagtgcctct acggcgacgg cccgactggc agccccttcc
     3901 tctacgaccg cgacgacggc gccaagctgc tggtcggcgg caacgacctg ggcgagcccg
     3961 gcgacctgcc ccgcaacgcc caggggatcg cactggtcgg cgacccgcgc aacgacgtcc
     4021 acctgttcgt ctcgcagctg cacctggcga tgctgaaggt ccacaaccgg ctggtcgacc
     4081 ggctgcggga ggacggcgag gccgaggctg agctgttcga cgccgcccgg cgcgcggtcg
     4141 cctggcacta ccagtggatc atcgtgaacg agttcctgcc gctggtggcc ggcgaggcgc
     4201 tggtcgacga gctgctgctg gaggggccgc gctggtaccg gccggagggg cagccacaga
     4261 tcccgctgga gttcgccgac gccgcctacc gctacggcca cagccagatc cgccgcggct
     4321 accgcctgcg ggcggacggc ccggcgctgc cgctgttccc cgacctgctc ggcttccagc
     4381 cggtgccggc cagccgccgg gtggactggt cgcagctgtt ccagttccct gacaggccag
     4441 tgccgcaggc ggccaagcgg atcgacgggc gcctggcgcg ctgcctgatc cagctgccga
     4501 tggcgatcac cggcgcggtg gaggtggcgg ccttccgctc gctcgcgacc cgcgacctgc
     4561 agcgcgggca gagctacggg ctgccgtcgg gggaggcgct cgcccggcat cttggcgccg
     4621 agccgctcag cgccgacgag gtcggcctgg ccgcccacgg ctgggagggc gagacgccgc
     4681 tgtggtacta cctgctccgc gaggcggcgg cgcggggcgg cggcgaccgc ctcggcccga
     4741 tcggcggccg catcgtcgcc gaggtgctgc tcggcatcat cgaccacgac ccggagtcct
     4801 accgcgccgt cgagcgggac tggcagccga ccctgcccgc cgcccggcca ggccgcttcg
     4861 gcatcgccga cctgctgctc gccgccgacg ccgctgatca ctgacctggg cggcggtggc
     4921 ccgtcgtgcc ggtgtggtca cagatgggtc cggcgccacg cggccttggc gagcacagca
     4981 tgctccgccg tgcccttggg gtcgttcagc gccgcatacg cgtccgccag tccctggtgg
     5041 gcgggcgtgg cgaagcttgc caggccgatc tggtcgaacg gtcgagcgcg gcgtggaagt
     5101 gggtcgtcgc gtcctgcatc cggcccgcct gaaggccgtg cagcgcccac tcaagctcga
     5161 ggcgcccatg gcgcagcgcg agcatcgccg cggccaagct gtcggcggct tcggaagctt
     5221 ccagcgccgc cctggcccgg cgcaggctcg gggcgctcgc ctggcgcctc gcgtccgttg
     5281 acccgtggcg cgactccacg cgggcacgca tggccttggc cagctcaacc tcgaagatcg
     5341 gcggtcggct gtcgccgggg ggccggtcgg cgacgctcag gtcggccgcg gcctcggcca
     5401 gcagacgcat gccgtccgag cccctgcgct cgcgttgcag gtaccggtga agtccaacct
     5461 gtgcccgcag cgcgtagacc ccgaggccga ggtcctgggc gtgagcctcc gcggcggagc
     5521 ctgcctcggc tttccccgcc tgtaccgcca ggtcctcgta ggcgagcttg gctcccttca
     5581 gcgcgctgaa cgcggcgtcg tgcctgcccg cgcccaggta cagggtggcg gcgacctcga
     5641 aggcggatcc ctgggactcc gggtcggggc tgtccccgcg accccacggc atgccgaggc
     5701 gcgtgccggt ggcaaccagc aggtcggctg accgccagtt cccgcctccg ttggtcagct
     5761 cctggtagat ggtgtcgcgg gggctcccgc gaggcgccag cgtttccagt gagccggcgt
     5821 cgttccgacg accggtggcg gtggcgggac ggggctggtc agcggcggca ggggcaacat
     5881 cccccggtct ccgttcctgc tgcccgcttt gaacgctccc gtccgccgcc accgccgcct
     5941 cctgctcacc gtcgcgtcgc cgctccgccg ccgccggcta tcttcgccat gcacgctcgg
     6001 aggcggcgac gcgaccgccg cctcctgctg ggtcagggcc ggctgtagga gaagaccacc
     6061 gtcaccgagg cggagacctc ctgggtgccc ggggagaccg ggaccgggtt ggtgccgccg
     6121 ctggaggccg gggcgcgggt ggtgagggtg aggatcgggg gttggccgcc gccctgctcg
     6181 tcgatggagg ccacgccggt gacggtggcg ccgatcaggc tggcggcggc gtcggccttg
     6241 gcgcgggcgt cgtcgatcgc ggcgtgcagc gcggcggcgt ggaacgcctt ggcgtcctcc
     6301 agcgagaagt tcgggccgta gctggagctg gcgccgttgt cgctgccgag cgccagcagc
     6361 gccccggcgc ggagaggtcg cggaccgtca cggtcagctc ctggctggcg gtgtagcggc
     6421 cggggtgctc gccgtcctgg ccggcccagc cgctgccggt ggcgaggtcg gcctgggcga
     6481 taccggcgtg ctggaaggcg gcgagcaggg tggtcatccg cgcgctcgcc tggttctgcg
     6541 cggcggcgag gctgctgccg ctgcccacgg tgctgaagtg gagggtcgcc gtgtcgggtt
     6601 tggcctggac cgtgccagtg ccggtgaagc gcaggtaggc ggcgagctgc ccgccctgcg
     6661 cctgcgcctg cgcctgcggc ggtgccagcg gggcggccag cggccgcgcc agcgccggcg
     6721 gggccgcctg ggagcgcggc gtcgcgctga cgggcggcgc ggtccgcagc gtcaccgcgc
     6781 cggcgatgcc ggtcgcgagg agcgccgcgg cggcgacgat ggcgcgctga cgggtcctgc
     6841 cgcgctggca ttggagctgg agccgggagc gtccagcagc gggggtgggg gcggaaggca
     6901 cggcggtcga cctccgaggg gtgggatggc cgggtgggct gcccggccgc gctgggctga
     6961 tcccttctgt ttcggccccg gccggtgaag ctgtagccgc gccggcgggt cagccggtca
     7021 ggccatcgcc gcggcagagg gcgccgcaca gctcggcggc ccgctgctcg tgctcgctgg
     7081 cgcgacgctg ggcgcccagg gctcggtagg acacggccag gccgtggtgg gcgcgggccg
     7141 cgaagccggc ctggtggagg tggtcgaagc cggccagggc gacggtgtag tacagccggg
     7201 cgctctccgg gctgcccccg gcgcggtcct ggt
//
LOCUS       DASWDC010000224         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1310449, whole genome shotgun sequence.
ACCESSION   DASWDC010000224 DASWDC010000000
VERSION     DASWDC010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310449"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VF995_07330"
     CDS             <1..1085
                     /locus_tag="VF995_07330"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9343409.1"
                     /translation="AFCQAVAALDRFDRASLYWAGRLTMVARKQDFDAYEAAFRAWFP
                     AERDRATDLIAEWASQMRPPEAEAAGVELLLEEGFAEEGWTPAAPAEQDADADDSEAI
                     IGVVASAAEVLHAKAFEDLTEAERLQANQLIRALALRLPNKRTRRRRPSPHGAQFDFR
                     RTVRASLRTQGEPFRRAWRDRRERPRPLVLILDVSGSMSSYSRALTQFGFAAIRAGKR
                     VEVFCFGTRLTRLTRALRRSEPDEALRQVAATVVDWDGGTRIGASLKELLDRWSQHAS
                     MRGAVVVLCSDGLERGDPRLLADQMARLSRLSHRLIWLNPLKGSPRYQPLARGMAAAL
                     PHVDLFLPGHNLASLEALCQVLEAQR"
     gene            1147..1668
                     /locus_tag="VF995_07335"
     CDS             1147..1668
                     /locus_tag="VF995_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX9343410.1"
                     /translation="MTEISTSPVTVSLTAAPLSLDAAFQAVLRPGCGGTALFVGTTRT
                     PNEGNHVEELEYEAYAEVALPELERVGQAALTRHGLGAAYLAHRVGAVPAGEASVIVA
                     ASAPHRAEAFAGCRELIDELKLTVPIWKKEHWDGGGRWVGIPVPDDDFEPDPDVLDPS
                     GGPAVEEVRHDRA"
BASE COUNT          212 a          636 c          633 g          242 t
ORIGIN      
        1 tggcgttctg ccaggcggtc gcggccctgg accgcttcga ccgcgccagc ctctactggg
       61 cggggcggct caccatggtc gcccgcaagc aggacttcga cgcctacgag gccgccttcc
      121 gcgcgtggtt cccggctgag cgggaccgcg ccaccgacct gatcgccgag tgggcctccc
      181 agatgcggcc gcccgaggcg gaggccgccg gcgtcgagtt gctgctggag gaggggttcg
      241 ccgaggaggg ctggaccccg gccgcgccag cggagcagga cgccgacgcc gacgacagcg
      301 aggcgatcat cggcgtggtc gccagcgccg ccgaggtgct gcacgccaaa gccttcgagg
      361 acttgaccga ggccgagcgc ctccaggcca accagctgat ccgggcgctc gcgctgcggc
      421 tgccgaacaa gcgcacccgc cgccgccgcc cctcgcccca cggcgcccag ttcgacttcc
      481 gccggaccgt ccgtgcctcc ctgcggaccc agggcgagcc gttccgccgc gcctggcgcg
      541 accggcgcga gcggccccgg ccgctggtgc tgatcctgga cgtgtccggg tcgatgtcgt
      601 cgtattcgag ggcgctgacg cagttcggct tcgccgcgat ccgcgccggc aagcgggtcg
      661 aggtgttctg cttcggcacc cgcctcacgc ggctcacgcg ggcgctgcgc cgcagcgaac
      721 ccgacgaggc gctgcgccag gtggccgcca cggtggtcga ctgggacggc ggcacccgca
      781 tcggcgcctc cctgaaggag ctgctggacc gctggtccca gcatgcctcc atgcgcggcg
      841 cggtggtggt gctctgctcc gacgggctgg agcgcgggga cccgcggctg ctcgccgacc
      901 agatggcgcg cctcagccgc ctgtcccacc ggctgatctg gctcaacccc ctcaagggca
      961 gcccccgcta ccagccgctc gcccgcggga tggccgccgc cctgccgcac gttgacctgt
     1021 tcctgcccgg ccacaacctc gccagcctgg aggccctgtg ccaggtgctg gaggcacagc
     1081 ggtgagcgcg ccggtgagcg cgacggtgag cgcggcgctg accggctctc ggtgtaccgt
     1141 gcggtgatga ccgaaatctc gacgtcgccg gtgacggtgt cgctgaccgc ggcaccgctg
     1201 tcgctggacg ccgcctttca ggcggtcctg cggcctggct gcggcgggac cgcgctgttc
     1261 gtcggcacga cgcggacgcc caacgagggc aaccacgtcg aggagctgga gtacgaggcg
     1321 tacgccgagg tggccctccc cgagctggag cgggtcgggc aggcggcgct gacccgccac
     1381 ggccttggcg ccgcctacct cgcccaccgc gtcggtgcgg tcccggcggg cgaggccagc
     1441 gtgatcgtgg cggcttccgc tccccaccgg gccgaggcgt tcgccggctg ccgcgagctg
     1501 atcgacgagc tgaagctcac cgtgccgatc tggaagaagg agcactggga tggcggcggc
     1561 cgctgggtcg gcatcccagt ccccgacgac gactttgagc ccgaccccga tgttcttgat
     1621 ccatcggggg gccccgcggt tgaggaggtt cgccatgacc gagcctgatc gcgtgatggt
     1681 ggacagtttc gggcgggtcg tgcgcgacct gcgcgtgtcg gtc
//
LOCUS       DASWDC010000225         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_889679, whole genome shotgun sequence.
ACCESSION   DASWDC010000225 DASWDC010000000
VERSION     DASWDC010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889679"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VF995_07340"
     CDS             complement(<1..223)
                     /locus_tag="VF995_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343411.1"
                     /translation="MPREPVEAKAIRYLTAGRLQVLEVTPQVVRAECAGSTGELHRVG
                     WWRGRWGCSCPAGRFSRTCCHLLALRLVVP"
     gene            complement(223..>1931)
                     /locus_tag="VF995_07345"
     CDS             complement(223..>1931)
                     /locus_tag="VF995_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9343412.1"
                     /translation="LDAQTFPPLRYAVPDLLPEGFALLVGPPKIGKSWFVINALLGRA
                     AGGYALGTIPVTEPRRVLYLALEDSDRRMQDRCRALLGTDEPIPPLFHYLTVVEPGQL
                     LATIDAFMTCHPQTALVVVDTLGKIMPPALPGESAYQRDYRVGSALQQLARDHPGLAV
                     VVCHHDRKATSSDFIDSVSGTHGLAGAADSVLVLTRDRSERAGLLKVAGRDIPEGEYA
                     LVFDDGRHWRLDGPDLVASAARARDLRATAGLGDRSAEVISFVGKHPQGATPAEVAAA
                     LGIEEKTVSAYLSRAVKTGRLSRPHRGLYTPVGSVGSVGSDGDDHSEPNTSNTSNTPS
                     GEAATADFQWSIEVEPVGPCSVCAAACCSRGPDGQPAHPGCWTRRTPPGVSDASAGPD
                     AAVTGSANGSANGYQFPTREQWTAERRTLYSDDACNAQAAVSRSALDHATPEEITETL
                     AALAEGRRRAHRDLRAARSDTDAGDSEDLAPLQEVHRRYGRAIKSLRDDQVPLATFEG
                     GELPELRHLVERYQAAVDAALATARRDVEARPVDDAAWAEELEHRQRVGRDHGITSMR
                     EA"
BASE COUNT          252 a          628 c          753 g          298 t
ORIGIN      
        1 caggcacgac cagccgaagg gcgagcaggt ggcagcaggt ccggctgaac cggccggcgg
       61 gacagctgca tccccagcga ccccgccacc agcccacacg atgcagctcg ccggtgcttc
      121 cggcgcattc ggcccggacc acctgcggcg tgacctccag gacctggagc cggcccgcag
      181 tgaggtagcg gatggccttt gcctcgaccg gctcgcgcgg catcacgcct cccgcatgct
      241 cgtgatcccg tggtcgcgcc cgacccgctg acggtgttcg agttcctcgg cccatgcggc
      301 atcgtcaacg gggcgggctt cgacatcgcg gcgggcggtg gcgagcgcgg cgtcgaccgc
      361 cgcctggtag cgctcaacga ggtgtcgaag ctccgggagt tccccgccct caaacgtcgc
      421 cagggggacc tggtcgtcgc ggagggactt gatcgcgcgt ccgtagcggc ggtgaacctc
      481 ctgcaggggc gccaggtcct cgctgtcgcc cgcgtcggtg tcgctccggg ctgcccggag
      541 gtcgcggtgt gcacggcggc ggccctcggc gagcgcggcg agggtctcgg tgatctcctc
      601 gggtgtggcg tggtcgagcg cgctcctgct caccgcggcc tgggcgttgc aggcgtcgtc
      661 gctgtagagg gtgcgccgct cggccgtcca ctgctcccgg gtcgggaact ggtagccgtt
      721 cgcgctgccg ttcgcgctgc cggttacggc ggcgtcgggg ccggcactgg catcggagac
      781 gcctggcggg gtgcggcgtg tccagcagcc cggatgggcc ggctggccgt cgggcccgcg
      841 ggagcagcac gccgccgcgc agactgagca ggggccgacc ggctcgacct cgattgacca
      901 ctggaagtca gcggtagcgg cctcaccaga aggggtgttg gaagtgttgg aagtgttggg
      961 ttccgaatgg tcgtcaccgt cgcttccaac acttccaaca cttccaacag gggtatagag
     1021 gccgcggtgt ggccttgaga gccgtcccgt cttgaccgca cggctgaggt aggcgctgac
     1081 cgtcttctcc tcgatgccga gcgcggcggc gacctccgcg ggtgtggcgc cctgcgggtg
     1141 cttgccgacg aagctgatga cctcggcgga ccggtcgccc agcccggcgg ttgcccgcag
     1201 gtcccgcgcc cgtgccgctg aggcaaccag gtcaggaccg tcgagccgcc agtggcggcc
     1261 gtcgtcgaag acgagcgcgt actcgccttc ggggatgtcg cggccggcga ccttcaacag
     1321 tccggcgcgt tcgctgcggt cgcgggtcaa caccagcacg ctgtcggcgg cgccggcgag
     1381 gccgtgggtg ccactgaccg agtcgatgaa gtcgctggag gtggctttgc ggtcgtggtg
     1441 gcagacgacg acggcgaggc cgggatggtc gcgggcgagc tgttgcagcg cgctgccgac
     1501 gcggtagtca cgctggtagg ccgactcgcc gggcagggcg ggcggcatga tcttgccgag
     1561 cgtgtcgacc accaccagtg cggtctgcgg gtggcaggtc atgaacgcgt cgatggtggc
     1621 caggagttgg ccgggttcga cgacggtcag gtagtggaac agcggcggga tcggctcgtc
     1681 ggtgccgagg agcgcccggc agcggtcttg catgcgccgg tcgctgtctt ccagggcgag
     1741 gtacagcacg cggcgtggct ctgtgaccgg gatcgtgccg agtgcgtagc cgccggcggc
     1801 gcggccgagg agggcgttga tgacgaacca ggacttgccg atttttgggg ggccgaccag
     1861 gagcgcgaaa ccctcgggga gcaggtcggg gacggcgtag cgcagcggcg ggaaggtctg
     1921 cgcgtcgagc c
//
LOCUS       DASWDC010000226         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_309593, whole genome shotgun sequence.
ACCESSION   DASWDC010000226 DASWDC010000000
VERSION     DASWDC010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309593"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VF995_07350"
     CDS             <1..208
                     /locus_tag="VF995_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEX9343413.1"
                     /translation="KALREAAGLQPGVELEITEDHGQIQIEVASQPIRLVERDGFLAA
                     EVEGDQGPPLTAEAVRDLLDGLRR"
     gene            205..609
                     /locus_tag="VF995_07355"
     CDS             205..609
                     /locus_tag="VF995_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9343414.1"
                     /translation="MITVDTSVTVAAFARWHVAHGVAREALKHVDRLIGHVAVETYSV
                     LTRLPPPRRVPAQLVIEFLGHSFPNPALTLPDDHYAELLRLAAAQNIVGGAIYDALIA
                     KTAGSHQATLLTLDARAASTYAAVGVDHRLLS"
     gene            complement(722..1708)
                     /locus_tag="VF995_07360"
     CDS             complement(722..1708)
                     /locus_tag="VF995_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9343415.1"
                     /translation="MAVETGDPIPVGTWGVASERAWRRWLRRVLAALLGALLLAVAAV
                     GFVAWSASSKLLLLPRQAVVETPARYHLAFRDVSFTSRDGVVLRGWFLPAGQGTQAGL
                     APVLVLSHGRGQSREEWLRKLPLFHRHGFAVLDFDYRACGLSGGRYNTAGYKETWDLL
                     AAVDFAKGQPGVDPGRIAVVGNSQGASTALLAAARDSDLRAVVEDSGFASLQQVIAYN
                     FHQEAGLPAFPFAPVTVLFAQLRAGADVSRVTPADVIRRIAPRPVLVIHGLRDTKVRP
                     DQAAKLYAKAGDPKAEWLVPTADHVGAFDVDPKGYERHVIGFLGQALGIPAA"
     gene            1859..2197
                     /locus_tag="VF995_07365"
     CDS             1859..2197
                     /locus_tag="VF995_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343416.1"
                     /translation="MRVLRIGVVGDYQPGNETHTATAAAVEHAAASRGWAAEVTWIPT
                     RAVMGAGEAEAEDAPEGAAARTLAGFDALWIAPGSPYQSMRGALAAITYARAPTTCRC
                     SAPAAASSTC"
     gene            2161..2637
                     /locus_tag="VF995_07370"
     CDS             2161..2637
                     /locus_tag="VF995_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX9343417.1"
                     /translation="MLGTCGGFQHMLIEFARKVAGIPDADHAEYNPDASRLLIGALAC
                     SLVGQTMTVTLVEGSVAQRAYGTASTTERYYCRFGLNPAYLPALQHAGLVVSGTDQDG
                     EVRIMELPGQRFFVATLFVPQTSSTPAAPHPLISAYLAAAQRAAARAALTASTPSQ"
     gene            2699..3844
                     /locus_tag="VF995_07375"
     CDS             2699..3844
                     /locus_tag="VF995_07375"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX9343418.1"
                     /translation="MGGIDGIGNTPIVRLEQLPGDGSAELWVKLEAANPTGSYKDRMA
                     LAMIDAAERDGRLQPGQLVVEYTGGSTGSSLAFVCAVKGYPLRIVSSDAFAVEKIRTM
                     QAFGAEVELISSPRGITPDLIPAMMARAGEIAAETGAFWTDQFNNADMLGGYQRLGEE
                     VLEQLPSPPGIGAFCAYVGTAGCFLGTSRALTAKFPELRRVAVEPAESAVLSGGPPGT
                     HHIEGGGIGRRPPLLQLDGFDEAVAVPEADAFAMARTAARREGVFSGPSTGANLVAAL
                     QLAQRLGPGPPGRHRPGRQRPQVPGRGRLLRQPRHPPAVSLVCHWASRATAARSSAAR
                     SSGESAAGLALLSLLALTLGERLFDAMRHAFHGKWWCCYSKRNRRAG"
BASE COUNT          579 a         1547 c         1416 g          579 t
ORIGIN      
        1 caaagctcta cgtgaagcag cagggctgca acccggcgtc gagcttgaga tcacggagga
       61 ccacgggcag atccagatcg aggtcgcctc ccagccaatc cggctggtcg agcgcgacgg
      121 cttcctcgcc gccgaagtcg agggcgacca aggccctccg ctaaccgcgg aagcggtccg
      181 cgacctgctc gacgggctgc gccggtgatc accgtcgaca cctcggtgac ggtggcggcg
      241 ttcgcccgat ggcatgttgc ccacggcgtc gcccgcgagg cgctcaagca cgtcgatcgc
      301 ctgatcggcc acgtcgccgt cgagacctac tcggtgttga cccggctgcc gcccccgcgc
      361 cgtgtgcccg cccagttggt catcgagttc ctcggccact ccttccccaa tcccgccctg
      421 acccttcccg acgaccacta cgccgaactg ctccgcctcg ccgccgccca gaacatcgtc
      481 ggtggggcga tttacgacgc gctcattgcc aagaccgccg gcagtcacca agcgacgttg
      541 ctcaccctcg acgcccgagc cgccagcacc tatgccgccg tcggcgtcga ccatcgcctg
      601 ctctcctgaa gctgggcgtc acggcaggtc atggctgtct accagcggca accgtcgtcg
      661 gccgtgatcg gcgcgatccg ctcagcgtgc cgcgcaggtc cgccctcaac acgatcctgc
      721 gtcaagcggc tgggatgccg agggcctggc cgaggaagcc gatcacatgg cgttcatagc
      781 ccttcgggtc gacgtcgaag gcgccgacgt ggtcggcggt ggggacgagc cactccgctt
      841 tcgggtcgcc cgccttggcg tagagcttgg cggcctggtc ggggcggacc ttggtgtcgc
      901 gcaggccatg gatcaccagc accgggcgcg gtgcgatccg ccggatgacg tcggccgggg
      961 tcacccggga gacgtccgcg cccgcacgga gctgcgcgaa cagcaccgtc accggggcga
     1021 acgggaaggc cgggaggccc gcctcctggt ggaagttgta ggcgatgacc tgctggaggg
     1081 aggcgaagcc ggaatcctcg accaccgccc gcaggtcgct gtcgcgggcg gcggccagca
     1141 gcgccgtcga cgccccctgg gagttgccga ccaccgcgat gcgccccggg tccacgcccg
     1201 gctgcccttt ggcgaagtcg accgcggcga gcaggtccca ggtctccttg tagccggcgg
     1261 tgttgtagcg gccgcccgac agcccgcagg cccggtagtc gaagtcgagc accgcgaacc
     1321 cgtgccggtg gaacagcggc agcttccgca gccactcctc ccggctttgt ccccgcccat
     1381 gcgacagcac cagcaccggc gccagccccg cctgcgtgcc ctggccggcc ggcaggaacc
     1441 agccgcgcag caccaccccg tcccgcgagg tgaacgagac gtcccggaac gccaggtgat
     1501 acctggccgg ggtctccacc accgcctggc gtggcagcag caacagcttg gagctcgccg
     1561 accaggctac gaaccccacc gcggccaccg cgagcagcag cgccccgagc agcgcggcga
     1621 ggacccgccg cagccagcgc cgccacgccc gctccgacgc caccccccag gtccctaccg
     1681 gaatgggatc cccggtctca accgccaccg caacctcctc cttggcacct gctggagcag
     1741 ttctacacga tccgtcccct tccttcgccg cggatctccg gatctgtgga tattcggtgt
     1801 tcgccgccgc atcggcgacg gtagggtggg ccagcgagcc agccaagagg gaggcgtcat
     1861 gcgtgtgctc aggatcggcg tggtcggcga ctaccagccc ggcaacgaga cccacaccgc
     1921 caccgccgcg gccgtcgagc atgccgccgc gtcccgtggc tgggccgccg aggtcacctg
     1981 gatcccgaca cgggcggtca tgggcgcggg cgaggccgag gccgaggatg cgccggaggg
     2041 cgccgccgcc cggacgctcg ccgggttcga cgccctgtgg atcgcacccg gcagccccta
     2101 ccagagcatg cggggcgccc tcgcggcgat cacctacgcc cgcgcaccaa cgacctgccg
     2161 ttgctcggca cctgcggcgg cttccagcac atgctgatcg agttcgcccg caaggtcgcc
     2221 ggcatccccg acgccgacca cgccgaatac aacccggacg cctcccggct gctgatcggc
     2281 gcgctggcct gctcgctggt cggccagacc atgaccgtca ccctggtcga gggctcggtc
     2341 gcccagcggg cctacgggac cgccagcacc accgagcgct actactgccg cttcggcctg
     2401 aatcccgcgt acctcccggc cctccagcac gccggcctgg tggtcagcgg caccgaccag
     2461 gacggcgagg tccgcatcat ggaactgccc ggccagcgct tcttcgtggc gaccctgttc
     2521 gtgcctcaga cctccagcac ccccgccgcc ccacatcccc tgatcagcgc ctacctggcc
     2581 gccgcccagc gggctgcggc acgcgcggcg ctgaccgcct cgacaccgtc gcagtgaccg
     2641 ggtttctcag cggcgcagcg gcgcaccggc gctagctggc cagacgatcg gagggagcat
     2701 gggcgggatc gacggcatcg gcaacacccc gatcgtccgg ctggagcagc tacccggcga
     2761 cggcagcgcc gagctctggg tgaagctgga ggcggccaac ccgaccggct cctacaagga
     2821 ccggatggcc ttggccatga ttgacgctgc cgagcgcgac gggcgcctcc agccggggca
     2881 gttggtggtc gagtacaccg gcggcagcac cgggtcgtcg ctcgcgttcg tctgcgccgt
     2941 gaagggctat ccgctgcgga tcgtgtcatc cgacgccttc gccgtcgaga agatccggac
     3001 gatgcaggcc ttcggcgccg aggtcgagct gatctccagc ccgcggggca tcactcccga
     3061 cctgatcccc gccatgatgg cccgtgccgg cgagatcgcc gcggagaccg gcgcgttctg
     3121 gaccgaccag ttcaacaacg ccgacatgct cggcggctac cagcgcctgg gcgaggaggt
     3181 actggagcag ctgccctccc cgccgggcat cggcgcgttc tgcgcctacg tcggcacggc
     3241 tggctgcttc ctcggcacca gccgcgccct gaccgcgaag ttcccggagc tgcgccgcgt
     3301 ggcggtcgag ccggcggagt cagcggtcct ctccggcggc ccgcccggca cccaccacat
     3361 cgagggcggc ggcatcggcc ggaggccgcc cctcctccaa ctcgacggct tcgacgaggc
     3421 cgtcgccgtc cccgaagccg acgccttcgc catggcccgc accgccgccc gccgcgaggg
     3481 cgtcttctcc ggcccctcga ccggcgccaa cctggtcgcc gccctccaac tcgcccagcg
     3541 cctcggcccc gggccgccgg gtcgtcaccg tccaggccga cagcggcctc aagtacctgg
     3601 ccgggggcgc ctactgaggc agccgcgtca cccgcccgcg gtgagcttgg tgtgccactg
     3661 ggcctcgcgg gcgacggcgg cccgatcatc agcggcccga tcatcagggg agtcagccgc
     3721 cgggctggcc ctcctgtcgc tgcttgcctt gacattgggc gaacgcttgt tcgatgctat
     3781 gaggcatgcg tttcacggaa agtggtggtg ctgctactca aagagaaatc gaagggctgg
     3841 ctgagcgggt tcggccgctg gcgttggccg atgagcgggt ggttcccgtg cctgcggcgt
     3901 tccaggggct ggtgcctggg ggaggactgc ggcgggggag cacggtgagc gtccaggggt
     3961 cggtggcgct ggcactggcg ctggtcgcgg gggcttcggc agcggggtcg tgggtggtgg
     4021 cggcggggct gcccgacctc ggggtggtgg cggcggcgga ggccggcatc gtcctggagc
     4081 ggttcgcgct ggtgcccgcg gttggttccc gggtgtgggc g
//
LOCUS       DASWDC010000227         8026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1227748, whole genome shotgun sequence.
ACCESSION   DASWDC010000227 DASWDC010000000
VERSION     DASWDC010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227748"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VF995_07380"
     CDS             <1..830
                     /locus_tag="VF995_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX9343419.1"
                     /translation="AEAHATETLSFGQVVAQSSNIGTIEVAQRLGRDQLVRAEQAFGY
                     GRRTGAGVPGESAGILNPVSTWHCTDWVNAIGQGVAVTVLQMTRVYATVANGGVMVEP
                     RLLKGTIDSSGTLNPAPASARRRVISSATAKTLTSILEGVVRKGGTAPAAALADWTVA
                     GKTGTAQKPGPDGRYMPGKFVASFIGFAPAERPAVVIAVVLDEPTAGSHFGGVVAAPV
                     FRQIADYELHHLAIPPTVPLAQAAAARGALPAADGDAPGSPAAAPAAQLTQTNQAPP"
     gene            1068..2540
                     /locus_tag="VF995_07385"
     CDS             1068..2540
                     /locus_tag="VF995_07385"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX9343420.1"
                     /translation="MTDVVLDTRAVSDGALFCCVPGRRVDGHDLAGQAVAAGAAALCA
                     ERPVEASVPQLLVPSVRAALAPLAAAFFDHPASRLDLVGITGTNGKTTTAFMLDAIIR
                     AAGLRSGLIGTVEVRVGDERRPVLHTTPEAPDLQRVLAEMADSGVQAAAVEVSSHALA
                     YGRVDATHFRAAIFTNLTPEHLDFHTDLEGYFQAKRLLFTPAFADLAVVNLDDPYGRR
                     LAGELARTGDVPVVTIGRSQEAAWRASAIEASLAGTSFQLDGPGPGPGGPRQAQVRLR
                     LAGYFNVANALGALAAADALGIDLGTAVQGLARLRGVPGRFERVEGGQRFTVLVDYAH
                     TPDSLESVLRAARTVASGRVLLVFGCGGDRDRAKRPLMGEIAGKLSDLAVVTSDNPRS
                     EDPGAIIAQILEGLRRSAGPDRFQVVADRRAAIRTALALATPGDLVIVAGKGHELGQV
                     FADGRKIPFDDRVVVAEELARLGHRPAGPAGAPDESGGAL"
     gene            2537..3988
                     /gene="murF"
                     /locus_tag="VF995_07390"
     CDS             2537..3988
                     /gene="murF"
                     /locus_tag="VF995_07390"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX9343421.1"
                     /translation="MIPLTLQEVAEAAGGVLRDADGTAVSTGMGTDSRTIRPGDLFVA
                     LPGSQTDGRRFAAAAVAAGAVAAVVQTQTERGGAGEPGTPEFHGVPRVEVSDPLRALM
                     ALAAAVRGRSRARVVGITGSTGKTTTKDLLHAALSTRLRTRANQGSFNTEVGLPLTLA
                     RLEPDTEALVVEMGARGPGHIAELAALAHPEVGVVLNVGVAHIGMFGSREAIAAAKGE
                     LVEALPASGTAVLNADDPLVAAMAGRTAARVLRFGAAAADVGAEAVTLDDAGCAHLKL
                     RTPAGSVALTLPAPGEHLVSDALAAAAAAHVLGLDPDQIAAGLAAARLSPGRMQVGRR
                     ADGLTVINDAYNANPASLAAALKVLAAARHKEGGRRVAVLGEMAELGDHAEAEHNQAG
                     RLATRLGIDRLIGVGPAGQWIVTAARLEGMSPEEALVVPNVTEALALLTPWLTPADVV
                     LVKASRVVALDRLAEQLLSGEGDGNGDDWRVPA"
     gene            3985..5061
                     /gene="mraY"
                     /locus_tag="VF995_07395"
     CDS             3985..5061
                     /gene="mraY"
                     /locus_tag="VF995_07395"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX9343422.1"
                     /translation="MIGILIAAAVALTVSIIGTPLAIRAFRRRGLGQLIHVDLPDAHQ
                     TKRGTPTMGGAVVILGAVVAYLAVHLVSGRNAPVTPGGLLVIITMVGTGAVGFVDDFL
                     KSRNQRSLGLTKTEKFIGQAVCALVLGLGAWYWAHSFHGISFVRPIIGTTLPLIVYLV
                     WVFVLLSGVSNAVNLTDGMDGLASGATVLVMSAYLVIAFWEFRHLCTLPPVNAPACYQ
                     FPRGGSPQDLAIVAAGMMGATAGFLWWNAPPARIFMGDTGSLSLGGTLAGLALFTNTQ
                     LLLAVIGGLYVVEALSVIIQVASYRFFQRRVFKIAPIHHHFELSQWPEFTVIVRFWIL
                     AGLAVAFGLGLFYAEFLAQGGGSG"
     gene            5072..6520
                     /gene="murD"
                     /locus_tag="VF995_07400"
     CDS             5072..6520
                     /gene="murD"
                     /locus_tag="VF995_07400"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX9343423.1"
                     /translation="MSQPDPARPVAEWPAHCSPGWFAGLPVLVCGLGVSGRAAATHLA
                     AFGADVLACDDATNLDVSGLLAAGVKTHLGGAAVELLDGRRLVVVVPGVPERHPLPRT
                     ALAAGIPVWSEVELAARLAATSMVGVTGTNGKTTTVELAAAMLSASGLRTVAAGNVGT
                     PLIDAVLAAPAPEALVVELSSFQLRFTHTLRLAAGAWLNFAPDHLDWHPDLPSYAAAK
                     ARVWANQTADDWSVYAADDPVVAANAAHSLGTPVPFTLGRAAPGGLGVEAGVAVSRIP
                     GLEGGLWRAAALRLPGRHNLANALAASALSLALGGHPAAMARAIAAFRGGPHRLTSVG
                     EIRGVAFVDDSKATNPHAAARALASYQRVVWIAGGRNKGLDFDELAAGARAQLVGVVL
                     LGEAAGELEEALERAGYDGPVARAASIGEAVMVGLGLAEPGDTVLLAPACASFDMFSS
                     YVERGDAFTAAVARLAARYQGPDHGGGRPVRP"
     gene            6492..>8026
                     /gene="ftsW"
                     /locus_tag="VF995_07405"
     CDS             6492..>8026
                     /gene="ftsW"
                     /locus_tag="VF995_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX9343424.1"
                     /translation="MGVAVLSVREATRHPARAGTQARSRPRGARLRNGLGAFSSQHLL
                     LVVVCALLAVGLVMVLSASSVLAYARFHDSYAYFKKQLLWVTLGLIAMLAVSRVDYRR
                     WRRLGAPLLVASTLGLFIVLVHPGAVTAYGATRWIPIGFGITLQPAEFAKLALLLFSA
                     DVLTRKRRLIGDVRHLLVPVLPVTLVLAALVMAEPDLGTTMLLSAIAFGMLFIAGTPL
                     PLFTAMGVAGLGGGLGLIMLAPYRRSRFFSFVNPARDYQDGGWQFIQGSRALGSGGLF
                     GVGLGASKQKWFYVPNAHTDFIFAVIGEELGLLGTLAIIGLFLVIAYTGIRVARRAPD
                     PFGRYLAGGITLWLVVQAVVNIGAVVGVLPITGVPLPLVSFGGSSMMVMLTAIGILLN
                     VARQEVAEAPAPLRATMAAAAAGRRRPAAVTAASSAMPGLAGARTTSTKAKASAKASA
                     KANAKAKVQSKPQSKPQSTSQGKAHGQPKAPGNPAARRQAPAPSASARRPRPPGTGRS
                     ADRS"
BASE COUNT          957 a         3188 c         2764 g         1117 t
ORIGIN      
        1 acgccgaggc gcacgccacc gagacgctct cgttcgggca ggtggtggcg cagtcgtcca
       61 acatcggcac catcgaggtc gcccagcggc ttggccgcga ccagctcgtc cgggccgagc
      121 aggcgttcgg ctacgggcgc cgcaccggcg cgggcgtgcc gggggagagc gccggcatcc
      181 tcaacccggt cagcacctgg cactgcaccg actgggtcaa cgccatcggg cagggcgtcg
      241 ccgtcacggt gctgcagatg acccgggtct acgccactgt cgccaacggc ggcgtgatgg
      301 tcgagccgcg cctgttgaag ggcaccatcg actccagcgg cacgctcaat cccgcacccg
      361 cctcggcgcg ccgccgggtg atctccagcg ccaccgccaa gaccctcacc agcatcctgg
      421 agggcgtggt gcgcaagggc ggcaccgccc ctgccgccgc gctggccgac tggaccgtgg
      481 ccggcaagac cgggaccgcc cagaagcccg gcccggacgg ccgctacatg cccggcaagt
      541 tcgtggcctc cttcatcggc ttcgccccag ccgagcgccc cgccgtggtg atcgcggtgg
      601 tgctggacga gccgaccgcc ggctcccact tcggcggcgt ggtcgccgcc ccggtgttcc
      661 gccagatcgc cgactacgag ctgcaccacc tggccatccc acccaccgtc ccgctggccc
      721 aggccgcggc tgcccggggc gccctccccg ccgccgacgg cgacgccccc ggcagtcccg
      781 ccgccgcccc ggccgcccaa ctcacccaga cgaaccaggc cccgccatga ccccatgtat
      841 gaagagccgc cgatgagcta cccccccggc cgatccttgt taggctttgc ccggtcgccg
      901 cagctgtggc ggcaggtact gtcccggagc cccggcgttc ccgccgctgg aggctcagac
      961 cgtcgatgcg ggctcaactt tccatgacac tgacgccccg gtgcgtcccg ctcgcctcgc
     1021 tcgccgcggc agttggcggc gagcttcgca atggcgatgg gacgctggtg acggacgtcg
     1081 tcctcgacac ccgcgcggtc tcagacggcg ccctgttctg ctgtgtgccc ggccgccggg
     1141 tcgacggcca cgacctcgcc ggccaggcgg tcgcggccgg cgcggcggcg ctgtgcgccg
     1201 agcggcccgt cgaggccagc gtgccgcaac tgctggttcc ttcggtgcgg gccgcgctcg
     1261 cgccgctcgc cgccgccttc ttcgaccatc ccgcgagccg cctcgacctg gtcgggatca
     1321 ccggcaccaa cggcaagacc accaccgcct tcatgctgga cgcgatcatc cgcgccgccg
     1381 ggctgcgcag cggcctgatc ggcaccgtcg aggtgcgcgt cggcgacgag cggcgcccgg
     1441 tcctgcacac caccccggag gcgcccgacc tccagcgcgt gctggctgag atggccgaca
     1501 gcggcgtcca ggcggcggct gtggaggtct ccagccacgc cctcgcctac ggcagggtcg
     1561 acgccaccca cttccgcgcc gcgatcttca ccaacctcac ccccgagcac ctcgacttcc
     1621 acaccgacct ggagggctac ttccaggcca agcggctgct gttcaccccc gccttcgccg
     1681 atctcgccgt cgtcaacctg gacgacccct acggccgccg cctcgccggc gagctcgcgc
     1741 ggaccggcga cgtcccggtg gtcacgattg ggcgaagcca ggaggccgcc tggcgcgcca
     1801 gcgcgatcga ggcgtcgctg gcgggcacca gcttccagct cgacggccct ggccccggcc
     1861 ccggcggccc gcgccaggcg caggtccggc tgcggctcgc cggctacttc aacgtcgcca
     1921 acgccctcgg cgcgctggcc gccgccgacg cgctcgggat cgacctcggc accgccgtcc
     1981 aggggctcgc caggctgcgt ggggtgccgg gccgtttcga gcgggtggag ggcggacaac
     2041 ggttcaccgt gctggtcgac tacgcccata cccccgactc gctggagagc gtcctgcgcg
     2101 ccgccaggac ggtcgcctcg gggcgggtgc tgctggtgtt cggctgcggc ggcgaccgcg
     2161 accgcgccaa gcggccgctg atgggcgaga tcgccggcaa gctcagcgac ctcgccgtgg
     2221 tcacctccga caacccgcgc tccgaggacc ccggggcgat catcgcccag atcctcgaag
     2281 ggctgcgacg cagcgccggc ccggaccggt tccaggtggt cgccgaccgc cgcgccgcga
     2341 tccgcaccgc cctcgccctg gccacccccg gcgacctggt gatcgtcgcc ggcaagggcc
     2401 acgagctggg ccaggtgttc gccgatggcc gcaagatccc gttcgacgac cgcgtcgtgg
     2461 tcgccgagga actcgcccgc ctcgggcacc gcccggcagg gccagcgggg gcccccgatg
     2521 aatcaggggg tgcgctgtga tcccgctcac cctccaggag gtcgcggagg cagccggggg
     2581 agtcctgcgc gacgccgacg gcacggccgt ctcgacgggc atggggactg actcccgcac
     2641 gatccgcccc ggcgacctct tcgtcgccct gcccggcagc cagaccgacg gccgccgctt
     2701 cgccgccgcc gccgtggcgg ccggggcggt cgccgcggtc gtccagaccc agacggagcg
     2761 cgggggagcc ggcgaacctg ggacccccga gttccatggg gtgccccgcg tcgaagtcag
     2821 cgacccgctg cgggcgctca tggccctcgc cgccgccgtg cggggccgca gccgcgcccg
     2881 ggtcgtgggg atcaccgggt cgaccggcaa gacgaccacc aaagacctgc tccacgccgc
     2941 gctctccacc cgcctgcgga ccagggccaa ccagggctcc ttcaacaccg aggtcgggct
     3001 gccgctgacc ctcgcccggc tggagcccga caccgaggcc ctcgtggtcg agatgggcgc
     3061 gcgcgggccc ggccacatcg ccgagctggc cgccctcgct caccccgagg ttggggtggt
     3121 gctcaacgtc ggcgtcgccc acatcggcat gttcggctcc cgcgaggcga tcgccgccgc
     3181 caagggcgag ctggtcgagg cgctccccgc cagcggcacc gccgtgctca acgccgatga
     3241 cccgctggtc gccgcgatgg ccggccggac cgccgcgcgg gtgctccgct tcggtgccgc
     3301 cgccgccgac gtcggcgccg aggcggtcac cctcgacgac gccggctgcg cccacctgaa
     3361 gctccgcacc cctgcgggga gcgtggcgct gaccctgccc gcccccggcg agcacctggt
     3421 cagcgacgcg ctggcagccg cagccgccgc ccacgtcctc ggcctggacc ccgaccagat
     3481 cgccgccggg ctcgccgcgg cgcggctctc gcccggccgt atgcaggtcg gccgccgcgc
     3541 cgacggcctc accgtgatca acgacgccta caacgccaac cccgcctccc tggccgccgc
     3601 cctcaaggtc ctcgccgccg cccgccacaa ggagggcggc cgccgggtcg ccgtcctcgg
     3661 cgagatggcc gagctcggcg accacgccga ggccgagcac aaccaggccg gccgcctcgc
     3721 cacccgcctc ggcatcgacc gcctgatcgg tgtcgggccc gccggccagt ggatcgtcac
     3781 ggccgcccgc ctggaaggca tgtcgcccga ggaggcactc gtcgtcccca acgtcactga
     3841 ggcgcttgcc ctcctcacac cgtggctcac ccccgccgac gtcgtgctgg tcaaagccag
     3901 ccgcgtggtc gccttggacc ggctcgccga gcagctgctc agcggcgaag gcgacggcaa
     3961 cggcgacgac tggagggtgc ccgcgtgatc ggcatcctga tcgccgcggc cgtggcgctg
     4021 accgtctcga tcatcggcac gccgctggcg atccgcgcgt tccggcggcg tgggctcggc
     4081 cagctgatcc acgtcgacct gcccgacgcc caccagacca agcgcggcac ccccaccatg
     4141 ggtggcgcgg tggtcatcct cggcgcggtc gtcgcctacc tggccgtcca cctggtctcc
     4201 ggccgcaacg cgccggtcac ccccggtggg ctgctggtga tcatcaccat ggtcgggacc
     4261 ggcgcggtcg gcttcgtcga cgacttcctg aagagccgca accagcgcag cctcggcctg
     4321 accaagaccg agaagttcat cgggcaggcg gtctgcgcgc tggtgctggg gctcggcgcc
     4381 tggtactggg cgcactcctt ccacggcatc tcgttcgtgc ggccgatcat cggcaccacc
     4441 ctgccgctga tcgtctatct cgtctgggtg ttcgtgctgc tgtcgggggt gtccaacgcc
     4501 gtcaacctga ccgacgggat ggacgggctc gcctccgggg cgaccgtgct ggtgatgagc
     4561 gcctatctgg tgatcgcctt ctgggagttt cgccacctct gcaccctgcc gccggtcaac
     4621 gcgcccgcct gctaccagtt cccgcgcggc ggctcgccgc aggacctggc gatcgtggcg
     4681 gcggggatga tgggcgcgac cgccggcttc ctgtggtgga acgccccgcc cgcgcggatc
     4741 ttcatgggcg acaccggctc gctgtcgctc gggggcaccc tcgccgggct ggcgctgttc
     4801 accaacaccc agctgctgct cgccgtgatc ggtgggctgt atgtggtgga ggccctctcg
     4861 gtgatcatcc aggtcgcctc ctaccggttc ttccagcgcc gggtcttcaa gatcgccccg
     4921 atccaccacc atttcgagct gtcgcagtgg cccgagttca ccgtgatcgt ccgcttctgg
     4981 atcctcgccg ggcttgcagt tgccttcggc ctcgggctgt tctatgccga gttcctcgcc
     5041 caagggggtg gaagtggctg acgaccacgt cgtgtcccaa cctgatccgg cccgcccggt
     5101 cgccgagtgg cccgcgcact gctcgcccgg atggttcgcc ggtctgccgg tgctggtctg
     5161 cggcctcggc gtctccggca gggccgccgc cacgcatctg gcagccttcg gcgccgacgt
     5221 gctcgcctgc gacgacgcca ccaacctcga cgtcagcggc ctgctcgccg ccggggtcaa
     5281 gacccacctc ggcggcgcgg cggtggagct gctcgacggg cgccggctgg tggtggtcgt
     5341 cccgggcgtg cccgaacggc atccgctgcc gcggaccgcc ctcgccgccg gcatcccggt
     5401 gtggagcgag gtcgagctgg ccgcccgcct cgccgccacc tcgatggtcg gcgtcaccgg
     5461 caccaacggc aagaccacca ccgtcgagct ggccgccgcg atgctgtccg ccagcggcct
     5521 gcgcaccgtc gccgccggca atgtcggcac cccgctgatc gacgcggtgc tggccgcgcc
     5581 ggcgcccgag gcgctggtgg tggagctgtc cagcttccag ctccgcttca cccacaccct
     5641 gcggctcgcc gccggggcct ggctgaactt cgcccccgac cacctcgact ggcatcccga
     5701 cctgcccagc tacgccgccg ccaaggcgcg ggtgtgggcc aaccagaccg ccgacgactg
     5761 gtcggtctac gccgccgacg acccggtggt ggccgccaac gcggcccact ccctgggtac
     5821 cccggtcccg ttcaccctcg gccgcgccgc gcccggcggg ctcggggtcg aggcgggggt
     5881 ggcggtatcg cggatccccg gtctggaggg cgggctgtgg cgcgcggcgg cgctgcggct
     5941 gcccggccgg cacaacctcg ccaacgcgct ggcggcctcg gccctctccc tggcgctcgg
     6001 cggccacccc gccgccatgg ccagggcgat cgccgccttc cgcggcggcc cgcatcggct
     6061 caccagcgtc ggcgagatcc gcggggtcgc cttcgtcgac gactccaagg ccaccaaccc
     6121 ccacgcggcc gcccgcgccc tggcctccta ccagcgggtc gtctggatcg ccggggggcg
     6181 caacaagggc ctcgacttcg acgagctggc ggccggcgcg cgcgcccagc tggtcggggt
     6241 ggtgctgctg ggggaggccg ccggggagct ggaggaggcg ctggagcggg ccggctacga
     6301 cgggccggtg gcgcgcgccg cctccatcgg cgaggcggtg atggtcggcc tcggcctcgc
     6361 cgaaccgggc gacaccgtgc tgctcgcgcc cgcctgcgcc agtttcgaca tgttcagctc
     6421 ctacgtcgag cgtggcgacg ccttcaccgc ggccgtcgcc cgcctggccg cccgctacca
     6481 gggcccggac catgggggtg gccgtcctgt ccgtccgtga ggccacccgg cacccggccc
     6541 gcgcggggac ccaggcgcgg tcgcggccac gcggcgcgcg gctgcggaac gggctcggcg
     6601 ccttctccag ccagcatctg ctgctggtgg tggtgtgcgc gctgcttgcc gtcgggctgg
     6661 tgatggtgct gtcggcctcc tcggtgctgg cctacgcacg gttccacgac tcctacgcct
     6721 acttcaagaa gcagctcctg tgggtcaccc tcggcctgat cgcgatgctg gcggtgagcc
     6781 gtgtcgacta ccggcgctgg cgccgcctcg gcgcgccgct gctggtcgcc tcgacgctgg
     6841 ggctgttcat cgtgctggtc cacccgggcg cggtcaccgc ctacggcgcc acccgctgga
     6901 tcccgatcgg gttcggcatc accctgcagc cggccgagtt cgccaagctg gcgctgctgc
     6961 tgttctccgc cgacgtgctg acccgcaagc gccgcctgat cggcgacgtc cgccacctgc
     7021 tggtgccggt gctgccggtg acgctggtgc tggccgcgct ggtgatggcc gaaccggacc
     7081 tcggcaccac catgctgctg tcggcgatcg ccttcgggat gctgttcatc gccgggacgc
     7141 cgctgccgct gttcaccgcc atgggcgtcg ccggcctcgg cggcggcctc gggctgatca
     7201 tgctcgcccc ctaccgccgc tcccgcttct tctcgttcgt caacccggcc cgcgactacc
     7261 aggacggcgg ctggcagttc atccagggca gccgcgcgct cggctccggc gggctcttcg
     7321 gtgtcggcct cggcgccagc aagcagaagt ggttctacgt gccgaacgcc cacaccgact
     7381 tcatcttcgc ggtgatcggc gaggagctgg gcctgctcgg caccctggcg atcatcgggc
     7441 tgttcctggt catcgcctac acggggatcc gggtggcgcg ccgcgcgccc gacccgttcg
     7501 gccgctacct ggccggcggc atcaccctgt ggctggtcgt ccaggcggtg gtcaacattg
     7561 gcgcggtggt cggcgtgctg ccgatcaccg gtgtgccgct gccgctggtg tcgttcggcg
     7621 ggtcgagcat gatggtgatg ctcaccgcca tcggcatcct cctcaacgtc gcccggcagg
     7681 aggtcgccga ggcgcccgca ccgctgcggg cgaccatggc cgcggcggcg gcgggccgcc
     7741 gccgccccgc cgccgtgacc gccgcttcct ccgccatgcc cggcctcgcc ggagcccgca
     7801 ccacctccac caaggccaag gcgagcgcca aggcgagcgc caaggccaat gcgaaggcca
     7861 aggtccagag caagccccag agcaagcccc agagcaccag ccaaggcaag gcgcacggcc
     7921 aaccaaaggc accaggcaac ccagcggccc ggcggcaggc gccggcgccc tcggcctcgg
     7981 cgcgccggcc ccgccccccc gggaccgggc gttccgctga ccgttc
//
LOCUS       DASWDC010000228         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_890458, whole genome shotgun sequence.
ACCESSION   DASWDC010000228 DASWDC010000000
VERSION     DASWDC010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890458"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..666)
                     /gene="nucS"
                     /locus_tag="VF995_07410"
     CDS             complement(7..666)
                     /gene="nucS"
                     /locus_tag="VF995_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEX9343425.1"
                     /translation="MRLVIARCSVDYAGRLSTHLPLATRLLIVKADGSVLVHADGGGY
                     KPLNWMTAPCTLNQNDTAGVWRVANRSGETMTITIDEILHDSAHELGHDPGLVKDGVE
                     RHLQELLAERPQALGDRWAVVRREYPTDIGPVDLLCRDEHGGYVAVEVKRRGEIDGVE
                     QLTRYLVRLQGPLGQVWGVLAAQAFAPQARVLAADRGIRCVELDYDELRGLEPTTPRL
                     F"
     gene            844..1896
                     /locus_tag="VF995_07415"
     CDS             844..1896
                     /locus_tag="VF995_07415"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9343426.1"
                     /translation="MRIALVAPPWFSVPPDGYGGIESVVALLADGLAERGHEVTLFAP
                     PGSRTRARLVAPLGEEQSPPAGAVGDPWYEAAHAVAAYERAEEFDLLHDHTGPVGASI
                     GAVSGATVVHTLHGPFTGPAKLLYGRLARKLWFVAISESQRSFAPPGLRWAGVVYNGI
                     AMDRYPFAAGRDRGDYLVFIGRVDEEKAPDLAVEAARRAGRKLLMCCKIQNEREERYW
                     AEKVEPLLGRDGADVEVLGECDHDDKTRLLGGAAAVLFPVQWPEPFGLVMVEAMACGT
                     PVVAWRNGSVPEVVEDGRSGFMVDSMDEFVAAIGKVDQLDRAAARDHVARRFSAEAMV
                     DGYEEAYRRALAGHEG"
BASE COUNT          263 a          621 c          886 g          339 t
ORIGIN      
        1 cggccctcag aacagccggg gagtggttgg ctccaggccg cgcagctcgt cgtagtcgag
       61 ctcgacgcag cggatgccgc ggtcggcggc cagcacccgg gcctgcggcg cgaacgcctg
      121 ggcggcgagg accccccaga cctggccgag gggcccctgg aggcggacga ggtagcgggt
      181 cagctgctcg acgccgtcga tctcgccgcg gcgtttgacc tcgacggcga cgtagccgcc
      241 gtgctcgtcg cggcacagca ggtccactgg gccaatgtca gttgggtatt cacggcggac
      301 cacggcccag cggtcgccga gcgcttgggg gcgttcggcg agcagctcct ggaggtggcg
      361 ttcgacgccg tccttgacca ggccggggtc gtggccgagt tcgtgggcgg agtcgtggag
      421 gatctcgtcg atggtaatgg tcatggtttc gccgctgcgg ttggcgaccc gccagacgcc
      481 ggccgtgtcg ttctggttga gcgtgcaggg cgcggtcatc cagttgagcg gtttgtagcc
      541 gccgccgtcg gcgtggacca ggacggagcc gtcggctttg acgatcagca ggcgagtggc
      601 gagcggcagg tgggtgctga ggcggccggc gtagtcgacg ctgcagcggg cgatgacgag
      661 gcgcatgggg ggaggatagc cgagccgggg ggcggcgtcg ccggtggcgt cctgggtcca
      721 ggtcctgggt tcaggtcctg ggtccaggtc ctgggtccag gtcctggctt ggttcggcgc
      781 gcggcttgcg ggggaagtcg gcgggggagt ttgcgcttgg tccgagtctg gaggaggagt
      841 cgcgtgcgca tcgcgctggt cgcgccgccc tggttttctg tgccacccga tggctatggc
      901 gggatcgaat cggtggtggc gctgctggcc gacggcctgg ccgagcgggg ccatgaggtg
      961 acgctgttcg ccccgccggg gtcgcggacc agggcgcggc tggtcgcgcc gctgggtgag
     1021 gagcagtccc cgccagccgg cgcggtcggg gacccgtggt atgaggcggc ccatgcggtg
     1081 gccgcctacg agcgggcaga ggagttcgac ctgctccacg accacaccgg cccggtgggg
     1141 gcgagtatcg gcgcggtgag cggcgcgacg gtggtgcaca cgctgcacgg gcccttcacc
     1201 gggccggcga agctgctgta tgggcggctc gcccgcaagc tgtggttcgt ggcgatctcc
     1261 gagagccagc ggtcgttcgc gccgccgggg ctgcgctggg cgggggtggt ctacaacggc
     1321 atcgcgatgg atcgctaccc gttcgccgcc gggcgggacc ggggagacta cctggtgttc
     1381 atcggccggg tcgatgagga gaaggcgccg gacctggcgg tggaggcggc gcggcgtgcc
     1441 ggccgcaagc tgctgatgtg ctgcaagatc cagaacgagc gggaggagcg ctactgggcc
     1501 gagaaggtcg agccgctgct ggggagggac ggcgccgacg ttgaggtgct gggggagtgc
     1561 gaccatgacg acaagacccg gctgctcggc ggcgccgcgg cggtgctgtt cccggtgcag
     1621 tggccggagc cgttcgggct ggtgatggtg gaggcgatgg cctgcgggac gccggtggtc
     1681 gcctggcgca acggctcggt tcccgaggtg gtggaggacg gccgttccgg gttcatggtc
     1741 gattcgatgg atgagttcgt cgccgcgatc ggcaaggtgg atcagcttga ccgcgcggcg
     1801 gcgcgcgacc acgtggcgcg gcgcttctcg gcggaggcga tggtggacgg ctacgaggag
     1861 gcctaccggc gcgcgttggc cggccacgag gggtgagcgc cgggcggtta tgatggcggc
     1921 gccatgctgc cggagaatca atacctgccg accgggaata tggtctgaat ttcgcctgcc
     1981 agggcggtta ctctgtgctg agatgagcgg gtcttcccgc tgccgctggt gcggctgtct
     2041 ggtcggagtc ggatcgggga ctgatgagcg tggcggatcc gcgagcgccg cgagcgccgc
     2101 gagcgccca
//
LOCUS       DASWDC010000229         8686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1061491, whole genome shotgun sequence.
ACCESSION   DASWDC010000229 DASWDC010000000
VERSION     DASWDC010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8686
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061491"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VF995_07420"
     CDS             complement(<1..511)
                     /locus_tag="VF995_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9343427.1"
                     /translation="MAEQLYRRRWQALVILSLSLVVIGMDNTILNVALPTLVRDLGAS
                     ASQLQWMVDAYTLAFAGLLLTMGALGDRFGRRLLLDIGLLVFVGGSIASAWAGSSGML
                     IATRTVMGVGGAMIMPATLSIITDIFPLGTERDRAIGVWAAMAGLGIVLGPVIGGWLL
                     AHFWWGSVFL"
     gene            774..1745
                     /locus_tag="VF995_07425"
     CDS             774..1745
                     /locus_tag="VF995_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX9343428.1"
                     /translation="MVSLLGTRDELLKLIEAAFESTVFVRGNEITITGEPGDAARVGR
                     LFEELIHLLEQGNRLTAETVGRSIDMVLEGNGDRPSQVFGDAVLTVRGNRSITPKTAG
                     QKRYVDAIRKNTVIFSIGPAGTGKTYLAMAVAVQALLTKQVNRLILTRPAVEAGEKLG
                     FLPGTLSEKIDPYLRPLYDALYEMIDAEQVARLIERGTIEVAPLGYMRGRTLNSAFIV
                     LDEAQNTTPEQMKMFLTRLGMNSKAVITGDVTQIDLPSGQHSGLNVVREILTGIEGLA
                     FMYLSSRDVVRHRIVQEIVEAYHRYDEARAAPAQRQAPGPAVSRGRS"
     gene            1759..2322
                     /gene="ybeY"
                     /locus_tag="VF995_07430"
     CDS             1759..2322
                     /gene="ybeY"
                     /locus_tag="VF995_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX9343429.1"
                     /translation="MSVFVANEQTEQSVDEEHLALLAQLTMEAEGVEESEVSILVVDR
                     TVMTQLNKRYMDEDGPTDVLAFPIDGPSPASGPPEGPAGPRGFAEERLVDDYDDLDDD
                     EEDDDAPWLLGDVVLCPAYAAENARGAGHSLGAELELLTVHGILHLCGFDHAEPDDER
                     DMRARTDELLGRWRSGSGSSPGARTRR"
     gene            2319..3659
                     /locus_tag="VF995_07435"
     CDS             2319..3659
                     /locus_tag="VF995_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX9343430.1"
                     /translation="MTALLAAQLHFSVHTGDLWTLVAVMLLTAVAAFLSMSETSITRT
                     NRIRAMHLAEEGRRGARQLLALVEQPARSLNLVLLLVLVVQYTATSLFTSLMERVVGG
                     GLGVIIAVVVMTLLTFIFAEVAPKTFAVQHTDRAALTVSPIVYFITRLPVLGPLTRIL
                     IGIGNLITPGKGLKTGPFVSEDELRAMVDEAEKEEVIEEEEREMIHSIFEFGDTIVRE
                     VMVPRPDMVVASVDRSLQEVLELILRSGYSRVPIYQRDIDDIVGLAYAKDVLRSLHDG
                     QTSKPLTEILRPAPVMPESMRAADCLREMRRRKSHMVIVIDEYGGTAGLVTIEDLLEE
                     IVGEITDEYDREEPNIEPLPDGDHRVNARLGIDEVNELLDVELPATEWDSIGGLLFNL
                     VGDVPREGQEVDFQGLRLRAERVQGRRVTRVRIHRLTPEEQEVASQTRTEARRP"
     gene            3656..4135
                     /locus_tag="VF995_07440"
     CDS             3656..4135
                     /locus_tag="VF995_07440"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX9343431.1"
                     /translation="MSHRPKAAAGVSDRELVAAANAARALAYTPYSGFRVGAALLTGD
                     GVVIPAGNVENASYGLTICAERAAVCAALASGQRRFVAIAVVTDRPDPVTPCGACRQV
                     LREFPEGPALRVLCAGAGAGSVDPGGGSPGPVLTLALDQLLPRSFGPEQLQAGEALP"
     gene            4132..5013
                     /gene="era"
                     /locus_tag="VF995_07445"
     CDS             4132..5013
                     /gene="era"
                     /locus_tag="VF995_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX9343432.1"
                     /translation="MKSGLVALAGRPNVGKSTLLNRLLGQKVAIMSDTPQTTRSVVRG
                     VLHRPEGQAVLVDTPGLHKPKTLLGQRLNDLARGTLAEVDLVCLLVDATAGVGRGDQF
                     LAAELAKVDTPRFCLVNKVDAASKEKVAAALVAAAGLGDWAEIVPLSARTGDQLDVLV
                     GLLFQYLPEGPPLYPDDQVSDEPEQHLVAELIREKALGLLREELPHSLAVVVDEMGPS
                     DDNPDLLVIHATLFVERSSQKPIILGRGGTLLRDIGTRARTELEALLGTRIYLDLHVK
                     VAKEWQRDPKQLQRLGF"
     gene            5435..7042
                     /locus_tag="VF995_07450"
     CDS             5435..7042
                     /locus_tag="VF995_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006551629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX9343433.1"
                     /translation="MLDQVDLVVDGDSRVGVLGRNGAGKSTLLRVLAGLEEPIGGVVE
                     RSPPELTAGYLPQELDVLPGETTAALLRRRAGVAAAEAGMEAALAATQRRAGPAELAA
                     YDEALARFLALGGDDHEVRAPKVLADLGLPEPEALLGMPVAALSGGQKVKVGLAAILL
                     ARFGVLLLDEPTNNLDFDGLARLERFVRGFPGAVALVSHDRAFLEATTNRIAELDLVS
                     HQLSEFGGGYGAYLEERGRRREQADARYEEARAERTRLEASMRQRKEWSVSRRGQRTT
                     DNDKSLAGRRLGRSTAAAAGAKALEKRIERLGDQEKPWEGWELRMSLRAGRRSGALVA
                     SLAGAVVQLGAFTLGPVDLELHWQDRVAVTGPNGAGKTTLLRLLTGALQPSSGSARLG
                     AGVVVGHLWQEGALVFGDPARTLLAAVRDQTDLNLEEARSLLAKFDLTAGHTGRTQAE
                     LSPGERARAGLAVLVARGTNLLVLDEPSNHLDLDALEELERALDDFDGTLLVVTHDRR
                     LLERLRLTRRFEVETGAVREVGVPARG"
     gene            7052..7792
                     /gene="recO"
                     /locus_tag="VF995_07455"
     CDS             7052..7792
                     /gene="recO"
                     /locus_tag="VF995_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX9343434.1"
                     /translation="MNLYREQGVVLRTYRLGEADRIVVLMTQGEGKVRAVAKGVRKTR
                     SRFGGRLELFSHVDLSLYRGRNLDTVTQVEVVDAFRHLREDYDRVVAGSAMLEAVDQV
                     AQEREAAVRVFLLLIRGLRALDGGPRDPSVVLDAFLLKLMALEGYKPSLAECAACGLH
                     NETGTALRSFSINRGGALCGTCRSGMESVLDPGTMPLLAGLMAGELDRAAVVVPAGVP
                     RGEAGTLVKSFVEFHLDRRLRSYPLVAR"
     gene            7792..8586
                     /gene="uppS"
                     /locus_tag="VF995_07460"
     CDS             7792..8586
                     /gene="uppS"
                     /locus_tag="VF995_07460"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX9343435.1"
                     /translation="MTARNPRALPGDLDPKGVPDHVAIVMDGNGRWAGARGLPRNKGH
                     AAGEQALFDTVEGALDAGISWLTVYAFSTENWRRPVSEVSFLMNFNQSLLVRRSHELH
                     ERNVRIRFIGRRDRVPPHVRRRMEEAEELTAADTQMTLAVALDYGGRDELARAVQRLV
                     ADVKAGNGPRRLDEKAIAARLDEPGMPDVDLLIRTAGERRLSNFLLWQLYYAEVYFTP
                     LCWPDFNRQQLFTALRDYQGRRRRFGAVPDVPGPPPGPATGAPTPR"
BASE COUNT         1309 a         3164 c         3008 g         1205 t
ORIGIN      
        1 ccaggaagac cgaaccccac cagaagtgcg ccagcagcca gccgccgatc accggcccca
       61 gcacgatccc gaggcccgcc atcgccgccc acacgccgat ggcgcggtcg cgctcggtgc
      121 cgagcgggaa gatgtcggtg atgatcgaca gggtcgccgg catgatcatc gcgccgccca
      181 cgcccatcac cgttcgggtc gcgatcagca tccccgacga ccccgcccag gcggacgcga
      241 tcgagccgcc cacgaacacc agcagcccga tgtccagcaa gagcctgcgc ccgaaccggt
      301 cccccagcgc ccccatggtc agcagcagcc cggcgaacgc gagggtgtag gcgtcgacca
      361 tccactgcag ctggctggcg ctggcgccca ggtcccggac cagcgtcggc agggccacgt
      421 tgaggatggt gttgtccatc ccgatgacga ccaggctcag cgacaggatc acgagcgcct
      481 gccagcgccg gcggtagagc tgttcggcca tcgtgtgtct cccttcggcg catcgcatat
      541 acgagtcagt attgtatttt aatcacgccg gcgggtgccc gcaagcagtt ccgtcaaacc
      601 agcccaaccc gtagggcagc ccccccgaga cggatatact cggtgggtcg ccggcccggc
      661 gggacgtggg ccggccctga cctctgtcct ctccggaggg actactccaa cacgtgcaac
      721 aacctcagac gacacgagtc acggtcaacg tgcgggttcc cggcaaccag tcgatggtca
      781 gcctcctcgg cacccgcgac gaactgctca agctgatcga ggcggcgttc gagagcaccg
      841 tgttcgtgcg cggcaacgag atcaccatca ccggcgagcc gggcgacgcg gcgcgggtgg
      901 gccgcctctt cgaggaactg atccacctgc tcgaacaggg caaccgcctg accgccgaga
      961 ccgtcggccg ctccatcgac atggtgctgg agggcaacgg cgaccgcccc tcccaggtgt
     1021 tcggcgacgc ggtcctgacc gtccgcggca accgctcgat cacccccaag accgccggcc
     1081 agaagcgcta cgtcgacgcc atccgcaaga acaccgtgat cttcagcatc ggccccgccg
     1141 gcaccggcaa gacctatctc gccatggccg tcgccgtcca ggccctcctc accaagcagg
     1201 tcaaccgcct gatcctcacc cgccccgccg tcgaagccgg cgagaagctc ggcttcctgc
     1261 ccggcaccct ctccgagaag atcgacccct acctgcgtcc cctctacgac gccctctacg
     1321 agatgatcga cgccgaacag gtcgcccgcc tgatcgagcg cggaacgatc gaagtggccc
     1381 cgttggggta catgaggggg agaacgctga actccgcttt catcgtgctt gacgaggcgc
     1441 agaacaccac gcccgagcag atgaagatgt tcctgacccg ccttggaatg aactccaaag
     1501 cggtgatcac cggagacgtc acgcagatcg atctgccgtc ggggcagcat tcggggttga
     1561 atgtggtgcg ggagatcttg acggggatcg aggggctggc gttcatgtac ctgtccagcc
     1621 gggatgtcgt gcggcaccgg atcgtgcagg agatcgtcga ggcgtaccac cgctacgacg
     1681 aggcgcgggc cgccccggcg cagcgccagg ccccgggccc ggccgtcagc cggggacgat
     1741 cgtgatgggt gatcgccggt gagtgtcttc gtggccaacg agcagaccga gcagtcggtc
     1801 gacgaggaac atctggccct cctcgcccag ctcaccatgg aagccgaggg ggtggaggag
     1861 tccgaggtct cgatcctggt ggtcgaccgg accgtcatga cccagctcaa caagcgctac
     1921 atggacgagg acgggcccac cgacgtgctg gccttcccga tcgacgggcc ctcgcccgcg
     1981 tcggggccgc cggagggccc ggcgggaccc cgcggcttcg ccgaggagcg tctcgtcgac
     2041 gactacgacg acctcgacga cgacgaggag gacgacgacg ccccctggct gctcggcgac
     2101 gtggtcctgt gcccggccta cgcggccgag aacgcccggg gagccggcca cagcctcggc
     2161 gccgagctgg agctgctgac ggtgcatggc atcctgcacc tgtgcggctt cgaccacgcc
     2221 gagcccgacg acgagcgcga catgcgcgcc cgcaccgacg agctgctcgg gcgctggcgc
     2281 tccggctccg gctccagccc gggagcgagg accaggagat gaccgcgctg ctcgccgccc
     2341 agctccactt ctccgtccac accggcgacc tgtggacgct ggtcgcggtg atgctgctca
     2401 ccgcggtcgc ggcgttcctg tcgatgtcgg agacctcgat cacccggacc aaccggatcc
     2461 gcgccatgca cctcgccgag gagggcaggc gcggcgcgcg ccagctgctg gcactggtgg
     2521 agcagcccgc ccgctccctc aatctggtcc tgctgctggt gctggtggtg cagtacaccg
     2581 ccacctcgct gttcacctcg ctgatggagc gcgtggtcgg cgggggcctt ggggtgatca
     2641 tcgcggtggt ggtgatgacc ctgctcacct tcatcttcgc cgaggtcgcc cccaagacct
     2701 tcgcggtgca gcacaccgac cgcgccgcgc tcacggtctc gccgatcgtc tacttcatca
     2761 cccgcctgcc ggtgctcggg ccgctcaccc ggatcctgat cgggatcggg aacctgatca
     2821 cccccggcaa ggggctgaag accgggccgt tcgtgtccga ggacgagctg cgcgcgatgg
     2881 tcgacgaggc cgagaaggaa gaggtcatcg aagaggaaga gcgcgagatg atccactcca
     2941 tcttcgagtt cggcgacacc atcgtccgcg aggtgatggt gccccggccc gacatggtgg
     3001 tggccagcgt cgatcggtcc ctgcaggagg tgctggagtt gatcctgcgt tccgggtact
     3061 cccgcgtccc gatctaccag cgcgacatcg acgacatcgt cggcctcgcc tacgcgaagg
     3121 acgtcctgcg cagcctccac gacggtcaga ccagcaaacc gttgacggag atcctgcggc
     3181 ccgcgccggt catgcccgag tcgatgcgcg ccgccgactg cctgcgcgag atgcgccgcc
     3241 gcaagagcca catggtgatc gtgatcgacg agtacggcgg caccgccggc ctggtcacca
     3301 tcgaggacct gctggaggag atcgtcggcg agatcaccga cgagtacgac cgcgaggaac
     3361 ccaacatcga gccgctgccc gacggcgacc accgcgtcaa cgcccggctc ggcatcgacg
     3421 aggtgaacga gctgctcgac gtggagctcc ccgccaccga atgggactcg atcggcggcc
     3481 tgctgttcaa cctcgtcggc gacgtccccc gcgaaggcca ggaggtcgac ttccagggcc
     3541 tccgcctgcg cgccgaacgg gtccagggcc gccgcgtcac ccgcgtccgc atccaccgcc
     3601 tcaccccgga agagcaagag gtcgcctccc agacccgcac cgaggcccgg cggccgtgag
     3661 ccaccgtcca aaggccgccg ccggggtctc cgaccgcgag ctggtcgccg ccgccaacgc
     3721 cgcccgcgcg ctggcctaca cgccgtactc gggcttccgc gtcggcgcgg cgctgctgac
     3781 cggcgacggc gtggtgatcc cggccggcaa cgtcgagaac gcctcctacg ggctcaccat
     3841 ctgcgccgag cgggcggccg tctgcgcggc gctcgccagc gggcagcggc gcttcgtcgc
     3901 catcgccgtg gttaccgacc gccccgatcc ggtcaccccc tgcggcgcgt gccgccaggt
     3961 cctgcgcgag tttcccgagg gcccggccct gcgggtcctc tgcgccggcg ccggtgccgg
     4021 cagcgtcgat ccgggtggag gcagcccggg gccggtcctc accctggccc tcgaccagct
     4081 cctgccgcgc agcttcgggc cggagcagct ccaggcgggg gaggcgttgc cgtgaagtcc
     4141 ggcctggtcg ccctcgccgg ccgcccgaac gtcggcaagt cgaccctgct caaccgcctc
     4201 ctcggccaga aggtggcgat catgtccgac acgccccaga ccacccgctc ggtggtccgc
     4261 ggcgtcctgc accgcccgga gggccaggcg gtcctggtcg acacccccgg cctccacaag
     4321 cccaagaccc tgctcggcca gcgcctcaac gatcttgccc ggggcaccct cgccgaggtc
     4381 gacctggtct gcctgctggt cgacgccacc gccggggtcg ggcgcggcga ccagttcctc
     4441 gccgctgagc tcgccaaggt cgacactccc cgcttctgcc tggtcaacaa ggtcgacgcc
     4501 gcctccaaag agaaggtggc cgcagccctg gtggccgccg ccggcctcgg cgactgggcc
     4561 gagatcgtgc cgctgtcggc ccgtaccggc gaccagctcg acgtcctggt cggcctgctc
     4621 ttccagtacc tgccggaagg ccccccgctc taccccgacg accaggtctc cgacgagccc
     4681 gaacagcacc tggtcgccga actgatccgc gagaaggccc tcggcctgct ccgcgaggag
     4741 ctgccccact ccctcgcggt ggtcgtcgac gagatgggcc ccagcgacga caaccccgac
     4801 ctgctggtca tccacgccac tttgttcgtc gaacgctcca gccagaaacc catcattctc
     4861 ggccgcggcg gcaccctcct ccgcgacatc ggcacccgcg cccgcaccga gctcgaagcc
     4921 ctcctcggca cccgcatcta cctcgacctc cacgtcaagg tcgccaaaga atggcaacgc
     4981 gaccccaagc aactccagcg tctgggattt tgagcggcat gcgcgaggac cgagctgtcg
     5041 accgcctgat cgccgccggg ctggcgaccc caccccggcg cccgaaggct gatctcgact
     5101 ggactccggt cgccagcgac ggcagcgtca ctgacctgat tgaccggtcg tctggttccg
     5161 agtcatcgac agaggcacca gctgtccttg ggtgtatcgg tcgacctgat cgaggcggcg
     5221 gcgcttcgtg agggcgacga cgacatcctc agcgaagccg ttgaggtgtg acagtaatgc
     5281 gccgcccggt cgccaagctg caggctgcca ttgagcgtta ccgctcccag cggtaacgat
     5341 ccgacggaac acccgggcgt ccagccgttg agtatggaag gcttcaagca tgctcgcggc
     5401 aagagggatc acggtcgttc ggggagcgca cctcgtgctc gaccaggtcg accttgtggt
     5461 ggacggtgac agccgggtcg gggtgctggg gcgcaacggg gcggggaagt cgacgctgct
     5521 gcgggtgctc gccgggctgg aggagccgat cggtggggtg gtggagcggt cgccgccgga
     5581 gctgaccgcc gggtatctgc cgcaggagct ggacgtgctg ccgggggaga cgaccgcggc
     5641 gctgctgcgg cgccgcgccg gggtcgccgc cgccgaggcg gggatggagg cggcgctggc
     5701 ggcgacccag cggcgggcgg ggccggccga gctggccgcc tatgacgagg cgctggcgcg
     5761 gttcctcgcg ctcggcgggg acgaccatga ggtccgggcg cccaaggtgc tcgccgacct
     5821 cggcctgccg gagccggagg cgctgctcgg catgccggtg gcggcgctgt cgggcgggca
     5881 gaaggtcaag gtcggcctgg cggcgatcct gctggcgcgg ttcggagtgc tgctgctgga
     5941 cgagccgacc aacaacctcg acttcgacgg gctggcgcgg ctggaacggt tcgtcagagg
     6001 gtttcccggc gcggtggcgc tggtcagcca tgaccgggcg ttcctggagg cgaccaccaa
     6061 ccggatcgcc gagctggacc tggtcagcca ccagctcagc gagttcggcg gcggctacgg
     6121 cgcctacctg gaggagcgcg ggcgccgccg cgagcaggcc gacgcgcgct acgaggaggc
     6181 cagggccgag cggacccggc tggaggccag catgcgccag cgcaaggaat ggagcgtcag
     6241 ccggcgcggc cagcgcacca ccgacaacga caagtcgctc gccgggcggc ggctggggcg
     6301 ctccaccgcc gccgcggccg gcgccaaggc cctggaaaag cggatcgagc ggctcggcga
     6361 ccaggagaaa ccctgggagg gctgggagct gcgcatgtcg ctacgggccg ggcgccgcag
     6421 cggcgcgctg gtcgccagcc tcgccggcgc ggtcgtccag ctcggcgcct tcacgctcgg
     6481 cccggtcgac ctggagcttc actggcagga ccgggtggcg gtgaccgggc cgaacggggc
     6541 gggcaagaca acgctgctcc ggctcctcac cggcgccctc cagccaagca gcggcagcgc
     6601 ccggctcggc gccggggtgg tcgtcgggca tctctggcag gagggcgcgc tcgtcttcgg
     6661 cgacccggcc cgcacgctgc ttgcggccgt ccgcgaccag accgacctga acctggagga
     6721 ggcccgctcg ctgctcgcca agttcgacct gaccgccggg cacaccggcc gcacccaggc
     6781 cgagctgtcc ccgggggagc gcgcccgcgc cgggctcgcc gtgctggtcg cgcggggcac
     6841 caacctgctg gtcctggacg agccctccaa ccacctcgac ctcgacgcgc tggaggagct
     6901 ggagcgcgcc ctcgacgact tcgacggtac cctgctggtc gtcacccacg accggcggct
     6961 gctggagcgg ctccgcctca ccaggcggtt cgaggtcgag acgggggccg tccgcgaggt
     7021 cggcgtcccg gcccgcggct agaatgacca ggtgaatctt tatcgcgagc agggggtggt
     7081 gctgcgcacc taccggcttg gcgaggcgga ccggatcgtc gtcctgatga cccaggggga
     7141 gggcaaggtc cgcgcggtcg ccaagggggt gcgcaagacg cggtcgcggt tcgggggccg
     7201 gctggagctg ttcagccacg tcgacctgtc gctgtaccgg ggacgcaacc tcgacacggt
     7261 cacccaggtt gaggtggttg acgccttccg gcacctgcgg gaggactacg accgggtggt
     7321 ggccggcagc gcgatgcttg aggcggtcga ccaggtcgcc caggagcggg aggccgccgt
     7381 gcgggtgttc ctgctgctga ttcgggggct gcgggccttg gacggggggc cgcgggaccc
     7441 gtcggtggtg ctggacgcgt tcctgctcaa gctgatggcg ctggagggct ataagccgtc
     7501 gctcgccgag tgcgccgcct gcgggctgca caacgagacc ggcacggcgc tgcggagctt
     7561 ctcgatcaac cgcggggggg cgctgtgcgg gacctgccgg tccgggatgg agtcggtgct
     7621 cgacccgggt acgatgccgc tgctggccgg cctgatggct ggggagctgg accgcgccgc
     7681 ggtggtggtg cccgccgggg tgccgcgtgg tgaggcgggg acgctggtga agagcttcgt
     7741 ggagttccac ctggaccggc ggctgcgctc ctacccgctg gtggcgcgct gatgacggcc
     7801 cgcaaccccc gggcgctccc aggcgacctg gatcccaagg gcgttcccga ccacgtcgcg
     7861 atcgtcatgg acggcaacgg ccgctgggcc ggcgcccgcg gcctgccgcg caacaagggc
     7921 cacgccgccg gcgagcaggc cctgttcgac accgtcgagg gagcccttga cgcgggcatc
     7981 tcctggctga cggtgtacgc cttctcgacc gagaactggc gccgccccgt cagcgaggtc
     8041 agcttcctga tgaacttcaa ccagtcgctg ctggtgcgcc gctcccacga gctgcacgag
     8101 cgcaacgtac ggatccgctt catcggccgc cgcgaccggg tccccccaca cgtccgccgc
     8161 cgcatggagg aagccgagga gctgaccgcc gccgacaccc agatgaccct cgcggtcgcg
     8221 ctcgactacg gcggccgcga cgagctggcc cgcgccgtcc agcgcctggt cgccgacgtc
     8281 aaggccggca acggtccccg ccgcctggac gagaaggcca tcgccgcccg cctcgacgag
     8341 cccggcatgc ccgacgtcga cctgctgatc cgcaccgccg gcgagcgccg cctctccaac
     8401 ttcctgctgt ggcagctcta ctacgccgag gtctacttca ccccgctgtg ctggccggac
     8461 ttcaaccgcc agcagctgtt caccgccctg cgcgactacc agggccgccg ccgccgcttc
     8521 ggcgccgtcc cggacgtgcc cggccctcca cccggtcccg ctacaggcgc gcccacgcct
     8581 cggtgaggac gctgcgcagc accgcttcga tctcgtcgaa atgggactgg tcgcagatca
     8641 gcggcgggga gagctggatc accgggtcgc cgcggtcgtc ggcgcg
//
LOCUS       DASWDC010000230         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_950254, whole genome shotgun sequence.
ACCESSION   DASWDC010000230 DASWDC010000000
VERSION     DASWDC010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950254"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VF995_07465"
     CDS             <1..802
                     /locus_tag="VF995_07465"
                     /inference="COORDINATES: protein motif:HMM:NF022932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9343436.1"
                     /translation="QQPMTGYALANATRIPQPKVYETLRRLVDKRVVVQTGADPARFV
                     AVPAEDLLGQLDGEFRRRLAGARVGLARLATAPERVAYRPLQSFDRWSAVAERASALL
                     EGAERHVYLSAHDDQLAGLLDAVSAADARGVRVDLLHFGPLALDLRNGSTLRHASTDG
                     VVYRRHQARHLALVADSRETLWALAPDGDHWDGVAATDPLLAALVKGYVRHDLYVQRI
                     FADFGETLRARYGPSMEELTLPDTPDAPDLPAEAPATPSARSRRRRRA"
     gene            839..>2209
                     /locus_tag="VF995_07470"
     CDS             839..>2209
                     /locus_tag="VF995_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9343437.1"
                     /translation="MPLDGVTPFPPEFAERYRRLGYWEDRPLGAVFSELFRRHGQRVA
                     MVADGERVTYEELDRRAERLALHLLELGFQPLDRVVVQLPNVAEFVYLYFALQRIGAI
                     PLMALAAHREHEVRHYVAFVEAKGYAIADRLRGFDFLAFADTILADAPGLQHVLVAGD
                     RPLPDDPRYVSLRELLASEPRLAPSALDELKIDPDDPCVFLLSGGTTGIPKVIPRSHN
                     DYVFNTKGTAEACGISADDTLLVCLPLGHNFPLGTPGMQAHFVAGGRIVLSPSTLARD
                     VLALVERERVTQLDSVPALLISWLNDPALGSHDLSSVRVIRVAGQKCPVEIKPRVERA
                     FPAAKVTEGFGMGEGLIMWTRFDDPEEVRFQTVGRPWCPDDEIRLVDDDGNEVPPGEP
                     GELLVRGPYTLRGYYHAPETNARAFTADGFYQSGDVMRLHPTGRYVVDGRKKDLINRG
                     GEKISAE"
BASE COUNT          304 a          867 c          735 g          303 t
ORIGIN      
        1 gcagcagccg atgaccgggt atgcgctggc caacgcgacc aggatcccgc agcccaaggt
       61 ctacgagacg ctgcgccggc tggtcgacaa gcgggtggtg gtgcagaccg gcgccgaccc
      121 cgcgcgcttc gtcgccgtcc cggccgagga tctgctcggg cagctcgacg gcgaattccg
      181 ccgccgcctg gccggcgcca gggtcgggct ggcccgcctc gccaccgcgc cggagcgggt
      241 ggcgtaccgg ccactccagt cgttcgaccg ctggtcggcc gtggccgagc gggccagcgc
      301 gctgctggag ggcgccgagc ggcacgtcta cctgtccgcc cacgacgacc agctcgccgg
      361 cctgctcgac gcggtcagcg ccgccgacgc ccgcggggtg cgggtcgacc tgctccactt
      421 cgggccgctc gccctcgacc tgcgaaacgg ctcgaccctg cgccacgcca gcaccgacgg
      481 ggtcgtctac cgccgccatc aggccaggca cctcgccctg gtcgccgaca gccgcgagac
      541 gctgtgggcc ctcgccccgg acggcgacca ctgggacggg gtggccgcca ccgacccgct
      601 gctcgccgca ctggtcaagg gctacgtccg ccacgacctc tacgtccagc ggatcttcgc
      661 cgacttcggc gagacgctcc gcgcccgcta cggcccgagc atggaggaac tcaccctccc
      721 cgacaccccg gacgctccgg atctgcccgc cgaagccccc gccaccccgt ccgcgcggtc
      781 ccgccgccgc cgccgcgctt gacctgcctg acccccaagt atcaggagga agcaccgcat
      841 gcctctcgac ggcgtcaccc cgttcccgcc ggagttcgcc gagcgctacc ggcggctcgg
      901 ctactgggag gaccgcccgc tcggcgcggt gttcagcgag ctgttccgcc gccacggcca
      961 gcgcgtcgcg atggtggcgg acggcgagcg ggtcacctac gaggagctgg accgccgcgc
     1021 cgagcgcctc gccctgcacc tgctggagct cggcttccag ccgctggacc gggtggtggt
     1081 gcagctgccc aacgtggccg agttcgtcta cctgtacttc gccctgcagc gcatcggcgc
     1141 gatcccgctg atggcgctgg ccgcccaccg ggagcacgag gtccgccact acgtcgcgtt
     1201 cgtggaggcc aaagggtatg cgatcgctga tcggctgcgc ggcttcgact tcctcgcgtt
     1261 cgccgacacg atcctcgctg atgcgccggg cctccagcac gtgctggtcg ccggcgaccg
     1321 cccgctcccg gacgatccgc gctacgtctc gctgcgggag ctgctcgcca gcgagccgcg
     1381 cctggcgccc tccgccttgg acgagctgaa gatcgacccg gacgacccgt gcgtgttcct
     1441 gctgtcgggc ggtaccaccg ggatcccgaa ggtgatcccg cgcagccaca acgactacgt
     1501 cttcaacacc aagggcaccg ccgaggcgtg cggcatcagc gcggacgaca cgctgctggt
     1561 gtgtctgccg ctcggccaca acttccccct gggcaccccg ggcatgcagg cccatttcgt
     1621 cgccggcggg cggatcgtgc tctccccgag cacgctcgcc cgcgacgtgc ttgcgctggt
     1681 ggagcgcgag cgggtaaccc agctcgacag cgttcccgcg ctgctgatca gctggctcaa
     1741 cgacccggcg ctggggagcc acgacctctc ctcggtccgg gtgatccggg tcgcgggcca
     1801 gaaatgcccc gtcgagatca agccccgggt cgagcgcgcc ttccctgccg ccaaggtcac
     1861 cgagggcttc ggcatgggcg aaggcctgat catgtggacc cgcttcgacg accccgagga
     1921 ggtccgcttc cagaccgtcg gccgaccctg gtgccccgac gacgagatcc gcctggtcga
     1981 cgacgacggc aacgaggtcc ccccgggcga gcccggcgag ctgctggtcc gcggacccta
     2041 tacgttgcgc ggctactacc acgcccccga gaccaacgcc cgcgccttca ccgccgatgg
     2101 cttctaccag tccggcgacg tgatgcgcct gcaccccacc ggccgctacg tcgtggacgg
     2161 ccgcaagaag gacctgatca accgcggcgg cgagaagatc agcgccgag
//
LOCUS       DASWDC010000231         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1229135, whole genome shotgun sequence.
ACCESSION   DASWDC010000231 DASWDC010000000
VERSION     DASWDC010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229135"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..1273)
                     /locus_tag="VF995_07475"
     CDS             complement(290..1273)
                     /locus_tag="VF995_07475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX9343438.1"
                     /translation="MGSPVPAAYGFDEDPATWRLLRARPPRPALDWAAEALGGGTVTA
                     VRALRGGLASAVHLLTVDQRVRVVLRRYVRPEVNLEEPEIARWEARALRFVAAVELPT
                     PTLLAVDATGAAAGVPAVLMSRLPGRVEWSPPHRDRWLLRLAEQLPPIHAAPLPLPGA
                     GTRADGAGAEAGNGAASIRPFAPYRQDRYELPAWARWPAVWARAVDWFHRPTPPDAGP
                     AVFVHRDYHPGNVLWRRGRVTGVVDWASASIGPAVVDVGHCRGNLFPYGLAVADRFTA
                     HWERQSGARYHPWADIVTIIGDLDGLRDDPRPASERRLVEDALARAVAELG"
     gene            1581..2063
                     /locus_tag="VF995_07480"
     CDS             1581..2063
                     /locus_tag="VF995_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9343439.1"
                     /translation="MIAVLNRTYETQICSIARTLEVVGERWTLLVLRDVLRGTRRFDE
                     LIQSLGVARNVLSDRLDRLVAHGLVEREPYQDHPPRFEYHPTAKGSELVVVLLGLMHW
                     GDRHLAGLAGPPVLTEHRDCGGAVVEQLACGSCGRMVPPDQLITRPGPGARAPDHAAV
                     "
     gene            2340..2540
                     /locus_tag="VF995_07485"
     CDS             2340..2540
                     /locus_tag="VF995_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343440.1"
                     /translation="MTRIGSALVIIWLIIGVAAAAQRHYLDNSRPSCAKVGTVAATVV
                     AGPLNYVGVNPKIKCRTPQPSK"
     gene            complement(2574..>3004)
                     /locus_tag="VF995_07490"
     CDS             complement(2574..>3004)
                     /locus_tag="VF995_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEX9343441.1"
                     /translation="LTVLTVLALAFVMNLSGQTVTLGQWMAGAGGFFAFLSPILGWLG
                     VAVSGSDTSSNSLFGLLQVTAGHQAGLSPTLLAAANSSGGVLGKMISPQNLAIGASAV
                     GLAGEEGDLFRKVLGWSLLFLLLMCVLVLLQSTVLSGMVV"
BASE COUNT          451 a         1097 c         1068 g          388 t
ORIGIN      
        1 gccccgactc caccgcggcg tccaccgcca ccacccacgc ccgaggctcg aaggggacgt
       61 ccagggtggc cagcagcacc ggccgccccg ccgacttgac cggcgtgctg gggaccttgg
      121 actcggggcg gatccgggtc gacatcgacc ctccacgagg gacggaggat gtggtgtcct
      181 accggaagag tctggcacgc atcaaccggc agcgcgttgc gcggagccgg gcaagctcct
      241 gccgtggtcg caactcgtgg tcgcagctcg tcgggtcggt gggctcgcct cagccgagtt
      301 cggcgacggc gcgggccagg gcgtcctcga ccaggcgccg ctccgaggcg ggtcgcgggt
      361 cgtcgcggag gccgtccagg tcgccgatga tggtcacgat gtcggcccat gggtggtagc
      421 gggcgccgct ctgccgctcc cagtgggcgg tgaagcggtc ggccaccgcc aggccgtacg
      481 ggaagaggtt gccgcggcag tggccgacgt cgaccacggc cgggccgatg ctcgccgacg
      541 cccagtccac caccccggtc acccgcccgc gccgccacag cacgttgccg gggtggtagt
      601 cacggtggac gaataccgca gggccggcgt ccggcggggt cgggcggtga aaccagtcga
      661 ccgcacgggc ccagaccgcc ggccagcgcg cccaggcggg cagctcgtac cggtcctggc
      721 ggtagggggc gaacgggcgg atcgacgcgg ccccgttgcc cgcctcggcc ccagcgccgt
      781 cggccctggt ccccgcgccg ggcaggggca ggggcgccgc gtggatgggc ggcagttgct
      841 cggccaggcg caggagccag cggtcccggt ggggtggcga ccactcgacg cggccgggaa
      901 ggcgtgacat caacaccgcc ggcacaccag ccgcggctcc ggtcgcgtcc acggcgagca
      961 gcgtcggggt gggcagctcc acggcggcga cgaaccgcag cgcccgggct tcccacctgg
     1021 cgatctccgg ctcttcgagg ttcacctctg gccggacgta gcgccgcagc acgacccgca
     1081 cgcgctggtc caccgtcagc aggtgcacgg cggaggccag cccgccccgc agggcgcgca
     1141 ccgcggtgac cgtgccgcca ccaagcgcct cggcggccca gtcgagggcg gggcgaggcg
     1201 gccgtgcgcg cagcagccgc caggtcgccg ggtcctcgtc gaacccgtag gcggcaggaa
     1261 caggactacc catgatcgat ctccccaaag gtgtcgtcgc tggcgggggt ccacgccagg
     1321 cggcggggga gatcgagcgt tctagggtca gccgaagccg ggtcttcgca tcaccactcc
     1381 tcaagcctcc tcaagccgag ctgccaagcc agtatcgccc gcccatggtg cggaggctag
     1441 atgcattcca aggaagttgc ttgacgaaac tttccagctc cgcatagagg ttcgtcaagc
     1501 aacttcctcg ccgtctcgct tgaagaccgg gcgagcgttt ctggaggcgg tagtagcgtc
     1561 acgaaactga ctgggatacg atgatcgccg tgctgaaccg aacctacgag acccagatct
     1621 gctcgatcgc ccgcaccctt gaggtcgtcg gcgaacgctg gaccctgctc gtgctccggg
     1681 acgtcctgcg cggcacccgc cgcttcgacg agctgatcca gagcctgggc gtcgcccgca
     1741 acgtcctcag cgaccgcctc gaccgcctgg tcgcccacgg cctggtggaa cgcgagccct
     1801 atcaggacca cccgccccgg ttcgagtacc acccgaccgc caagggcagc gaactcgtgg
     1861 tcgtcctgct cggcctgatg cactggggcg accgccatct cgccggcctc gccggcccgc
     1921 ccgtcctcac cgagcaccgc gactgcggcg gggcggtcgt cgagcagctt gcctgcgggt
     1981 cctgcggccg gatggtcccg cccgatcagc tcatcacccg cccgggcccg ggcgcgcggg
     2041 caccggacca cgccgccgtc tgagctgcgc cgcactggtg ctcgcggacg gcggggttgt
     2101 cgtcgccggg ctggcgagtg ccgcggagag cgtctccgcc ggcaccgtcc acagcctcgc
     2161 ggtcggcttc ccgcccgcgc ggtgagtacg ggggaagccg ctggcgcgcc tcccccgaag
     2221 gccccttggc ctgaccgcca ccgggtggtc gtcgtcatcg tcgtttgcgt ggctggcatg
     2281 tttgttcgcg agcgtgggca tacgtgccat acaggaccgc gcgggacagg gaggcagcga
     2341 tgacgcgcat cggatcggca ctggtgatca tctggctgat tatcggcgtg gccgcggcgg
     2401 cgcagcgcca ctacctcgac aactcacggc cgagctgcgc caaggtcggg acggtcgcgg
     2461 ccacggtcgt cgccgggccg ctgaactacg tcggggtcaa cccgaagatc aaatgccgca
     2521 ccccgcagcc gtcgaagtga cggccacggg gtagctccgc cgcaggctcg gcctcagacc
     2581 accatgccgg acaggacggt cgactggagc agcaccagga cgcacatcag cagcaggaac
     2641 agcaggctcc agccgagcac cttgcggaac aggtcgccct cctcgccggc gaggccgacc
     2701 gcgctggcgc cgatcgccag gttctgcggc gagatcatct tgccgagcac gccgccggag
     2761 ctgttcgccg ccgccagcag cgtcggcgac aggccggcct ggtgccccgc ggtcacctgc
     2821 agcaggccga acagcgagtt cgacgaggtg tccgagccgc tcaccgcgac cccgagccag
     2881 ccgaggatcg gcgacaggaa ggcgaagaac ccgcccgcgc ccgccatcca ctggccaagg
     2941 gtgaccgtct ggccggagag gttcatcacg aacgccagcg ccagcaccgt caggaccgtg
     3001 agga
//
LOCUS       DASWDC010000232         8409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1394050, whole genome shotgun sequence.
ACCESSION   DASWDC010000232 DASWDC010000000
VERSION     DASWDC010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394050"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VF995_07495"
     CDS             <1..193
                     /locus_tag="VF995_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343442.1"
                     /translation="PASRSPEETRRLLTSYRAGLEQGRLEGRRVGGDANGGDANGDPT
                     TANGSDTSTRNGGDDTRQG"
     gene            253..600
                     /locus_tag="VF995_07500"
     CDS             253..600
                     /locus_tag="VF995_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HEX9343443.1"
                     /translation="MVRPYAITRGRIRRVHGELELELEALVTTTSRGISATRLPNEQR
                     SIVLLCRDLLSIAEVAARLRLPVEVIRTLVEDMADDGLVELHRPSHHDDRPDLALLER
                     VLYGLQHLPTPAS"
     gene            850..1701
                     /locus_tag="VF995_07505"
     CDS             850..1701
                     /locus_tag="VF995_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9343444.1"
                     /translation="MPAILLGLSASVCWGTADFFGGLQSRRLSALAVALWSQLVGGSV
                     LLAALLLITDNRPLHPAAVAFGAAGGLCGGLALLLFYRALAEGAMSIVAPISACGAIV
                     PLAVASLGGTLPSPRAIAGIGATLVGVLLACRSRDDEQHPSSRPALVVALSLGAALGF
                     GLYYVMLNAGAATGSSPLRVIAGGRAALLVVLAMVTLLSRERAPWPGRDLPGLAVIGV
                     VDTLANGLFTIACARGNLGVVGVLGSMYPVATVMLARFVLAERLSRLQRAGVTLSLLG
                     VALVAVG"
     gene            complement(1734..2729)
                     /locus_tag="VF995_07510"
     CDS             complement(1734..2729)
                     /locus_tag="VF995_07510"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9343445.1"
                     /translation="MRVLVTGGAGYVGGVVALRLRDAGHEVASLTRPAGHGLGSGHGG
                     APRPGVTTLTGDLRDAARLGELVATFQPEGVCHLAARVRVRESLSDPLGYFETNLAGT
                     LHLLRALELESLPERTGQPPRLVFASAGAVYDATRGVPLREDAPVAPTTPYAASKLAA
                     EQLIAAQTATGRLAAVTLRTLVITGSGPDGCRPDDDRSRLLPKALAVAAGLEPVLAVN
                     GDGSAVREYTHVADVADAYLLALDAARPGEHQVFNIGSGIAATITDLVATVEAVTGRP
                     LPVIRRPAQPEPQTLQLDSGHARATLGWQPRYTSLRELIEDGWARMAATAGAQPE"
     gene            3040..4518
                     /locus_tag="VF995_07515"
     CDS             3040..4518
                     /locus_tag="VF995_07515"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX9343446.1"
                     /translation="MAAPIVAYTWVASATPTVLYVDGSNPSCTDSGSGTQSVPYCTIT
                     KGASVATAGQTVQVAAGTYPQLATVGHSGTAGSPIVLTPAPGATVTVTGQADAFKVSS
                     KSYVTIQGFTVTNTTSYGIYVSGSSNVVVSGNIVSDTAAAGIYVTSSSSSVTVDGNTV
                     SGSATYGIYDNGSPSSTISNNTVSGSTSYGIQAASTSGVTISGNNVSDSGQQVSGSTK
                     YGIKLTAMTGSLVSGNVSHDNSEAGVFLDATTNGVEVTGNQTYGNARGYARAAPGIDV
                     RGYSNTVDRNVSHDNEDTGLQFYSGSHDNLVVDNLTYDNGDHGIDDLGSTGQRIISNT
                     VYNNVAAGINVEGSSTGATIEDNISVDNGINSPRTKSNIRVDSNSTSGTTVNYNLVYL
                     SVSGQVQYVWGSTSYTSLSAFQSATGQESNGISQNPQWVSYGSNFHLTSSSPAIDSAD
                     SGVSGETSTDLEGNSRVDVAGVPNTGSGPRDYDDRGAYEYQP"
     gene            4602..6056
                     /locus_tag="VF995_07520"
     CDS             4602..6056
                     /locus_tag="VF995_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX9343447.1"
                     /translation="MNIALIVLDAVRADHVSCYGYERPTTPNLDAFAAAKTRYATVVS
                     PGVWTFPSMTSMFTGLYPSQHGMNRANRSVPPELKLLAERLQEGGYRTGGFSANPYVG
                     RTYGFERGFEVFREFWGMGMAGGGDRSAVRGAVNRSYQWLFPRVRGTLKRSQTLTRAY
                     QHYLRRQVEGRDKGAAALTDAAVEWIGQARQDYHPFFLYAHIMEAHAPLAPPSAHVRR
                     FVADDAAIERAREVNQDAMAYMAGANPLTDDQMRSLIDLYDASIGYGDELLGRLLAAL
                     GDHGDTVVLITADHGNCFGEHGILDHFFSVHEPLAAVPMVASHPEIASGVIDRPVGTI
                     DIAPTILEAAGLPTAGLAGVSLAAETDPRACLVTEFLDPPLERFNRFHRFDPAAFRRD
                     LRGLRRGDHKYIWASDGRDELYDLRTDPHETTNLADADPALLADLRQLHQNWLQTTAT
                     GASATATANGYESADDFEVEEDVAESLRALGYFD"
     gene            complement(6069..7160)
                     /locus_tag="VF995_07525"
     CDS             complement(6069..7160)
                     /locus_tag="VF995_07525"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX9343448.1"
                     /translation="MIPPLRELLQARWPALRPGRPAPAGAIVAGVDRSPAAKVTLVFF
                     DQAGELAAIAKVARSAVSEPALEQEHEALTHLWSLGGGAPSVRRQVPQPLLLERVAGR
                     LALVVSPLAGRPMTAAYYTPGHVADQATVAADFTLAATWLRAFQTETAAPLQPFDPNQ
                     LDRWVGQVIRDYRAQVGWSATEEKLFTEVNTRAEALLGLPLPTAAIHGDYWMGNLLVD
                     PSGQLRGVIDWERGRREGPCLLDIYKFPTSYGLYLDRAYPSGVVPGHPDRQRWQTRWP
                     HLGDWLHLAGFGYTYYGDGWFPQQIRSFIISHLDALSVPHAANAVFFPLFLAEQALLP
                     GHPKTRAANRAMLHAFGEDPTGTWLWTSG"
     gene            complement(7157..7849)
                     /locus_tag="VF995_07530"
     CDS             complement(7157..7849)
                     /locus_tag="VF995_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343449.1"
                     /translation="MAIEQPADVAVVDHRLLNWRFVVPSVPDGLLLLPAADEAGEVPG
                     AVICDPGAGGPGATLTKLGAGPYPAVVLADVGRWAGSRRSGPDLLGRAAAAVAPGGWL
                     YASFANPWYPLRSRSAALTLAAASRAVRREGLQPISGWLAFPDQRTSAYLVPADGNAE
                     LAYFMRTFFFPYASGSSARKVRIKQQALTLMRNAALLAPASVRPRFAPAFALVAERVA
                     ERVAERVAERPA"
     gene            complement(7875..>8409)
                     /locus_tag="VF995_07535"
     CDS             complement(7875..>8409)
                     /locus_tag="VF995_07535"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX9343450.1"
                     /translation="LLAHQIFRLIYTAKFDDAVVTYQVLALVIAIRMLGHTLGTSITA
                     ANRQTARTIVVGAAAFTNIALNLYFIPRWSYLGAAETTAITESGVFIAYVILARRFIN
                     PSKLVEAVALPALACVPMAAVLLALRGDVNLLVTIAAGAAVYLLGLAIIAAALVPREA
                     LRHPRAALNALVARATA"
BASE COUNT         1237 a         2971 c         3051 g         1150 t
ORIGIN      
        1 ccccgcctcg cgctcccccg aggagacccg caggctgctg accagctacc gtgctggcct
       61 ggagcagggc agactcgaag gcaggcgcgt gggcggcgat gccaatggcg gcgatgccaa
      121 cggcgacccc accaccgcca acggcagcga cacgagcacg aggaacggtg gtgacgacac
      181 ccggcaaggg tgagcatgga gcaacaaccc gggcaacggc acgacagcgg acgcgggcga
      241 gacccgggcc gggtggttcg cccctacgcg atcacccgag gccgcatccg ccgcgtccac
      301 ggtgagctgg aactggaact ggaagcgctg gtcaccacca cctcccgggg gatctccgcc
      361 acccgcctgc cgaacgagca gcgctccatc gtgctgctgt gccgcgacct gctgtcgatc
      421 gcggaggtgg ccgcgcggct gcggctcccg gtcgaggtga tccgcaccct ggtcgaggac
      481 atggccgacg acggcctggt ggagctgcac cgcccctccc accatgacga ccggccggac
      541 ctggccctgc tggagcgggt cctctacggc ctccagcacc tgcctacccc ggccagctga
      601 ccggcgccgc gccaagccaa gcttgacttg agtcggcggc acggcagctc cgcggtcctg
      661 gtcacgcggc ggggttcgtc aggtgcccaa aatgcctgag cacctctttc tggatggggc
      721 tgctggaacg agtgggaaag tcagccggag ccctggacag ggagtttcgc cggactaaga
      781 tagtgatcgt tatctctcac tctcccgatc ggtgccacgc cccgatccct cccccaggag
      841 cagaacggct tgcccgcgat cctgctcggt ttgagcgcca gcgtgtgctg gggaaccgcc
      901 gacttcttcg gcggcctcca gtcccgccgc ctgtccgcgc tggcggtggc gctgtggagc
      961 cagctggtcg gcggcagcgt gctgctggcc gccctgctgc tgatcaccga caaccgcccc
     1021 ctccatcccg ccgcggtcgc cttcggcgcg gccggtgggc tctgcggcgg cctcgcgctg
     1081 ctgctgttct accgggccct ggccgagggt gccatgtcga tcgtcgcgcc gatctccgcc
     1141 tgtggcgcga tcgtcccgct ggcggtcgcc tccctcggcg gcaccctgcc gagcccgcgc
     1201 gccatcgccg gcatcggcgc caccctggtc ggcgtgctgc tcgcctgccg ctccagggac
     1261 gacgagcagc acccgagcag ccggcccgcg ctggtggtgg ccctctcgct gggtgcggcg
     1321 ctcggcttcg gcctctacta cgtgatgctc aacgcgggcg ccgcgaccgg cagctcgccg
     1381 ctgcgggtga tcgccggcgg ccgggccgcg ctgctggtgg tgctcgccat ggtgacgctg
     1441 ctcagccgtg agcgcgcccc ctggcccggc cgggacctgc ccgggctggc ggtgatcggc
     1501 gtggtcgaca ccctcgccaa cgggctgttc acgatcgcct gcgcccgcgg caacctcggc
     1561 gtggtcggcg tcctcgggtc gatgtatccg gtggccaccg tgatgctcgc ccgcttcgtg
     1621 ctggccgagc gcctctcccg gctgcagcgc gccggggtca ccctctcact gctcggcgtc
     1681 gccctggtcg ccgtcggctg acccgtcggc tgacccgctg gctgacccgc tggctactcc
     1741 ggctgtgctc ccgccgtcgc tgccatgcgg gcccagccgt cttcgatcag ctcgcggagg
     1801 ctggtgtagc ggggctgcca gccgagcgtg gcgcgggcgt ggccgctgtc gagctggagg
     1861 gtctgaggct ccggctgggc agggcggcgg atgaccggga gggggcggcc ggtgacggcc
     1921 tcgacggtgg cgaccaggtc ggtgatggtg gcggcgatgc cgctgccgat gttgaagacc
     1981 tggtgctcgc cggggcgggc ggcgtcgagc gcgagcaggt aggcgtcggc gacgtcggcg
     2041 acatgggtgt actcgcggac ggcggagccg tcgccgttga cggcgaggac cggttccagg
     2101 ccggcggcga ccgcgagggc cttggggagg aggcggctgc ggtcgtcgtc ggggcggcag
     2161 ccgtcgggtc cgctgccggt gatcaccagg gtccggaggg tgacggcagc gaggcggccg
     2221 gtggccgtct gggcggcgat cagctgctcg gcggcgagct tggaggcggc gtagggggtg
     2281 gtcggcgcga ccggggcgtc ctcgcgcaag gggacacctc gggtggcgtc atagaccgcg
     2341 cccgccgagg cgaagaccag gcgtgggggc tggccggtcc gctctggaag ggactcaagc
     2401 tccagcgcgc gcagcaggtg gagggtgccg gccaggttgg tctcgaagta gccgagcggg
     2461 tcgctcaggg attcgcggac ccgtacccgg gccgcgaggt ggcagacgcc ctccggctgg
     2521 aaggtggcga ccagctcccc gagcctggcg gcgtcgcgga ggtcgccggt gagggtggtg
     2581 acgccaggcc gtggggcccc gccgtggcca gagccgaggc cgtggcccgc ggggcgggtg
     2641 aggctggcga cctcatgccc ggcatcgcgg aggcggagcg ccaccacccc gccgacgtac
     2701 cctgctcccc cggtgaccag cacccgcacg cgaacgcccc tccagttgct tgctccgctc
     2761 tctcacggca ccctcattcg ccgtatggtc ccgctgctca ggctacgccg cgcaggccgg
     2821 gccgataccc ccgttgcgtg ttgccgccac gtgccgttcc gcccgatcct tctcattggt
     2881 cgcggctctg ccagcttctg ctgccatgag ccaccaatcg tgttattcac gcgggctgcc
     2941 caaacgggga cacaacaggg gggcagtgcc actgtgaggg ggaggcccca gatggtcaag
     3001 ttcccgaggc acgccgcgcg ttggcttgtg ctggttgccg tggcggcgcc gatcgtggcg
     3061 tatacatggg tggcatcggc gacgccgacg gtcctctacg tcgacggcag taatccgagc
     3121 tgcaccgaca gcggctccgg gacccagtcg gtcccctact gcaccattac caagggcgcc
     3181 tcggtcgcca ccgccggcca gaccgtccag gtggccgcgg gcacctaccc gcagctcgcc
     3241 accgtgggcc actccggcac ggccggctcc ccgatcgtgc tgacgccggc acccggcgcc
     3301 accgtgacgg tcaccggcca ggccgacgcc ttcaaggtgt cgagcaagag ctacgtcacc
     3361 atccagggct tcaccgtcac caacaccacc agctacggca tctacgtcag cggctcctcc
     3421 aacgtggtgg tcagcggcaa catcgtcagc gacaccgccg ccgcggggat ctacgtcacc
     3481 agctcctcct ccagcgtgac ggtcgacggc aacaccgtca gcggcagcgc cacctacggg
     3541 atctacgaca acggctcgcc gagttcgacc atcagcaaca acacggtgag cggcagcacc
     3601 agctacggga tccaggcggc cagcacctcc ggggtcacca tcagcggcaa caacgtcagc
     3661 gacagcggcc agcaggtgtc gggctcgacc aagtacggca tcaagctgac cgcgatgacc
     3721 ggctcgctgg tgtcgggcaa cgtctcccac gacaactccg aggctggcgt cttcctggac
     3781 gcgaccacca acggcgtcga ggtgactggc aaccagacct acggcaacgc ccgcgggtat
     3841 gcccgcgcgg cccccgggat cgacgtgcgc ggctactcga acaccgtcga ccggaacgtc
     3901 agccacgaca acgaggacac cggcctgcag ttctacagcg gctcccacga caacctggtg
     3961 gtcgacaacc tcacctatga caacggcgac cacggcatcg acgatctggg gtccaccggc
     4021 caacggatca tctccaacac cgtctacaac aacgtcgcgg ccggcatcaa cgtggagggc
     4081 tcctcgaccg gcgcgacgat cgaggacaac atcagcgtcg acaacggcat caactcgccg
     4141 cgcaccaaga gcaacatccg cgtcgactcg aactcgacca gcggcaccac ggtcaactac
     4201 aacctcgtct acctgagcgt ctccgggcag gtccagtacg tgtggggctc caccagctac
     4261 acctcgctgt cggccttcca gtcggccacc gggcaggagt cgaatggcat ctcacagaat
     4321 ccccagtggg tgtcctacgg gtccaacttc cacctgacct ccagctcgcc cgcgatcgac
     4381 tcggccgact ccggcgtgag cggcgagacc agcaccgacc tggagggcaa ctcccgggtg
     4441 gacgtcgccg gggtgccgaa caccggcagc gggccgcggg actacgacga ccggggcgca
     4501 tacgagtacc agccatgacg ccgggtgtga cgcgacgccg ttacgctgga acctgagggt
     4561 caacggaagg gtcaacgttt tttgggagtt ggaggaacgg gttgaacatc gccctgatcg
     4621 tgctcgatgc cgtgcgcgcc gaccatgtct cctgctacgg gtatgagcgg ccgaccacgc
     4681 cgaacctgga cgcgttcgcc gccgccaaga cccgctacgc cacggtggtc tcgccggggg
     4741 tgtggacctt cccgtcgatg acgtcgatgt tcaccgggct gtacccgtcg cagcacggca
     4801 tgaaccgggc caaccggtcg gtcccgcccg agctgaaact gctcgccgag cggctccagg
     4861 agggcgggta ccgcaccggc ggcttctccg ccaaccccta cgtcgggcgg acctacgggt
     4921 tcgagcgggg cttcgaggtg ttccgggagt tctgggggat ggggatggcg ggcggcggcg
     4981 accgctcggc ggtgcgcggc gccgtcaacc gctcctacca gtggctgttc ccgcgggtac
     5041 gcggcaccct caagcgcagc cagaccctca cccgcgccta ccagcactac ctccgccgcc
     5101 aggtggaggg gcgcgacaag ggcgccgccg cgctcaccga cgcggccgtg gagtggatcg
     5161 ggcaggcccg ccaggactac cacccgttct tcctctacgc ccacatcatg gaggcgcacg
     5221 cgccgctggc accgccgagc gcgcatgtcc gccgcttcgt cgccgacgac gccgcgatcg
     5281 agcgggcccg cgaggtcaac caggacgcca tggcctacat ggccggtgcc aacccgctca
     5341 ccgacgacca gatgcgctcg ctgatcgacc tgtacgacgc cagcatcggg tacggcgacg
     5401 agctgctcgg ccggctgctg gcggcgctcg gcgaccacgg cgacacggtg gtgctgatca
     5461 ccgccgacca cggcaactgc ttcggcgagc acggcatcct cgaccacttc ttcagcgtgc
     5521 acgagccgct cgccgccgtc cccatggtgg ccagccatcc cgagatcgcc agcggcgtga
     5581 tcgaccggcc ggtgggcacc atcgacatcg cccccaccat cctggaggcg gcggggctgc
     5641 cgaccgccgg gctggccggc gtgtcgctcg ccgccgagac cgacccgcgc gcctgcctgg
     5701 tcaccgagtt cctcgacccg ccgctggagc gcttcaaccg cttccaccgc ttcgaccccg
     5761 ccgcgttccg gcgcgacctg cgcggcctgc gccgcggcga ccacaagtac atctgggcct
     5821 ccgacggccg cgacgagctg tacgacctgc gcaccgaccc acacgagacc accaacctgg
     5881 ccgacgccga cccggccctg ctcgccgacc tgcgccagct ccaccaaaac tggctgcaga
     5941 ccacggcaac gggggcgtcg gccaccgcca cggccaacgg gtacgagtcc gccgacgact
     6001 tcgaggtcga ggaggacgtg gcggagagcc tccgcgccct cggctacttt gactgagccg
     6061 ctgcgggctc agccagacgt ccaaagccag gttcccgtcg ggtcttcgcc gaacgcgtgg
     6121 agcatggcgc ggttggcggc gcgggttttg gggtggccgg ggaggagggc ctgttcggcg
     6181 aggaacaggg ggaagaagac ggcgttggcg gcgtggggga cgctgagggc gtcgaggtgg
     6241 ctgatgatga agctgcggat ctgctggggg aaccagccgt cgccgtagta ggtgtagccg
     6301 aagccggcga ggtggagcca gtcgccgagg tggggccagc gggtctgcca gcgctgccgg
     6361 tcggggtggc cgggcacgac cccgctcggg taggcgcggt cgaggtagag gccgtaggac
     6421 gtggggaact tgtagatgtc gagcaggcag ggtccctcgc gacggccacg ctcccagtcg
     6481 atcacgccgc gcagctgccc ggaggggtcg acgagcaggt tgcccatcca gtagtcgccg
     6541 tggatggcgg cggtgggcag cgggaggccg aggagggcct cggcccgggt gttgacctcg
     6601 gtgaacagct tttcctcggt ggcgctccag ccaacctggg cgcggtagtc gcggatcacc
     6661 tggccgaccc agcggtcgag ctggttgggg tcgaacggct ggagcggggc ggcggtctcg
     6721 gtctggaagg cccgcagcca ggtggccgcc agggtgaagt cggcggcgac ggtggcctgg
     6781 tcggcgacgt ggccgggggt gtaataggcg gcggtcatgg gccgtccggc gagcggcgag
     6841 accaccaggg caaggcgccc ggcgacccgc tccagcagca gcggctgcgg gacctggcgc
     6901 cgcaccgacg gtgcgccccc cccgaggctc cacaggtggg tgagggcctc gtgctcctgt
     6961 tcaagggcag gctcggagac ggcgctccgg gcgaccttgg cgattgccgc cagttcgccg
     7021 gcctggtcga agaagaccag ggtgaccttg gcggcgggtg agcggtcgac gccagccacg
     7081 atcgccccgg cgggggcggg acggccaggc cgcagcgccg gccagcgtgc ctggagcagc
     7141 tcccgcagcg gcgggatcac gctggacgct ccgcgacgcg ctccgcgacg cgctccgcga
     7201 cgcgctccgc gacgagggcg aacgccgggg cgaaccgggg ccgcacggat gccggggcga
     7261 gcagggcggc gttgcgcatc agcgtgagcg cctgctgctt gatccggacc ttccgggccg
     7321 aggagccgct ggcatacggg aagaagaagg tgcgcatgaa gtaggcgagt tcggcgttgc
     7381 cgtccgccgg gacgaggtag gcggaggtgc gctggtccgg aaaggcgagc cagccggaga
     7441 tgggctgaag gccctcgcgt cggaccgccc gggaggccgc cgccagcgtc agcgccgccg
     7501 agcgggaccg cagcgggtac caggggttgg cgaacgaggc gtagagccat ccgccgggcg
     7561 cgaccgccgc ggcggcgcga ccgagcaggt ccggcccgct ccgccgtgac cccgcccagc
     7621 ggccgacatc ggcgaggacc accgctgggt acggccccgc gccgagcttg gtcagcgttg
     7681 cgcccgggcc gccggcgccc gggtcgcaga tgaccgcgcc cgggacctcc cccgcctcgt
     7741 cggcggcggg gaggagcagc aggccgtccg gcaccgaggg aaccacgaaa cgccagttca
     7801 gcaggcggtg gtcgacgacc gcgacgtccg ctggctgctc gatcgccatc gcgctccctc
     7861 cttcctcttc gttgtcaggc ggtcgcccgt gccaccaggg cgttgagggc ggcgcgcggg
     7921 tggcggagcg cttcgcgggg caccagggcg gcggcgatga tcgccaggcc gagcaggtag
     7981 acggcggcgc cggccgcgat cgtcaccagc aggttgacgt cgccgcgcag ggccaggagc
     8041 acggcggcca tcggcacgca cgccagcgcc gggagcgcca ccgcctccac cagcttggac
     8101 gggttgatga agcggcgggc gaggatcacg taggcgatga acacgccgct ctcggtgatg
     8161 gcggtggtct cggcggcgcc gaggtaggac cagcgcggga tgaagtagag gtttagggcg
     8221 atgttggtga acgccgccgc gcccaccacg atcgtccggg cggtctggcg gttggccgcg
     8281 gtgatcgagg tgccgagcgt gtggccgagc atgcggatcg cgatcaccag cgcgagcacc
     8341 tggtaggtca ccaccgcgtc gtcgaacttc gccgtgtaga tcaggcggaa gatctggtgg
     8401 gccagcagg
//
LOCUS       DASWDC010000233         8359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1645161, whole genome shotgun sequence.
ACCESSION   DASWDC010000233 DASWDC010000000
VERSION     DASWDC010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645161"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..176)
                     /locus_tag="VF995_07540"
     CDS             complement(36..176)
                     /locus_tag="VF995_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343451.1"
                     /translation="MIAAALRLLATALLAGVLLVALVPVIVAGFGQGERAWRRAGVRR
                     GR"
     gene            complement(173..439)
                     /locus_tag="VF995_07545"
     CDS             complement(173..439)
                     /locus_tag="VF995_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343452.1"
                     /translation="MPDPNATPPATTTLDPAAKSLLQAICQLDELTSGHAVARSIAFA
                     QVAGAAAAVLRQDAALRHAAHAVWEGVASFTADGRNRYLLEGDP"
     gene            678..1358
                     /locus_tag="VF995_07550"
     CDS             678..1358
                     /locus_tag="VF995_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX9343453.1"
                     /translation="MFLDAGDTLLSPWPSFHERFQQVALEEGASFELAAVEAAWATAI
                     REALWPEDWTDPATQQEFWHGFYVEVLRHLPFGGDPAGIATAAFEVFSDPATYQLFDD
                     SRPALEAMAHRGLKLGVISNFEPWLQDILALQGVDHLFAAVVISGVVGIAKPDPGIFH
                     AALAQAGVEASEAVHVGDQPEIDAKAAEEVGIAPVLIDRFGRRPEPVPWPRIASLTEL
                     VDLLDQKG"
     gene            1366..2223
                     /locus_tag="VF995_07555"
     CDS             1366..2223
                     /locus_tag="VF995_07555"
                     /inference="COORDINATES: protein motif:HMM:NF022431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEX9343454.1"
                     /translation="MDRSFWRVIATDENALPQGEWVAELTPELLSWLSSSDAELRDEF
                     AYRILAGWIDRDQYKPDQLRTMAAQMTANLFVGLGEAGTESVFLRSYSALVLMEIVAY
                     DNANPFLETFEVESYLDAALDYLARERDLRAYVQGPGWANAIGHTADLLMMLARHPHL
                     GAGALERILEGIASKLLSGGAPPFVHHEDERLAYAVLNVLRRDLVGWVFLRAWMERFL
                     AAPGKDSWRASFSNEVEAAARANVTAFLRGLYFQILLTEDPPPTAPEVVEAILATLKG
                     IDIGYYDLA"
     gene            complement(2242..3522)
                     /gene="murA"
                     /locus_tag="VF995_07560"
     CDS             complement(2242..3522)
                     /gene="murA"
                     /locus_tag="VF995_07560"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX9343455.1"
                     /translation="MESFVIKGGQPLCGSIRPAGNKNAALPIISATLLCDGPVVLHNV
                     PRIRDVATLLQLVASTGAEVSDVGPNSVRIDPRGVRRTSLDPLLCSQIRASVLLAAPL
                     LARTGRCVLPSPGGDVIGRRRLDTHLLAFRALGARASFDHRVLELEAERLRGHPMFLD
                     EASVTATENAISAAVLADGQTVIGNAACEPHIQDLCRFLNACGARISGCGSNLLIIDG
                     VERLSGGEYSIQPDYIEIASFIGLAAISGGELVIEDVVADDLRPVAVGFHRLGVRWLY
                     EGKNLRVPREQELTIQDDLGAAIPKLDDGPWPAFPADLTSIALAIATQCRGTVLIFEK
                     MYENRLFFVDKLVSMGARVILCDPHRAVVTGPGRLFGERLESPDIRAGMALLLAALVA
                     DGESVIGNIGQIDRGYERIDERLRGLGASIERVR"
     gene            3736..5049
                     /locus_tag="VF995_07565"
     CDS             3736..5049
                     /locus_tag="VF995_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9343456.1"
                     /translation="MQTFAMLPQEAIMGKPHGHGRPYGRPQGRFRFAAALAVAALLLA
                     ACSSGKSSSPSGGNQAQNSKPIRIGASISRSGDFSDPAQLVLRGYKLWADQVNAKGGL
                     LGRKVEIVTVDDASSPNQAVTNYQNLITRDKVDLTFGPFSSLLTLPSAQVAKRYGYAF
                     LEPEGGGPKVFAAKLPNLFFVQPAPAVAQGQVFADYVLSLPAAQRPKTAAYPSLDDPF
                     ASPVAEAVRTRFEQAGIRTVYNHIYPAETTDLTPIVQKVAAAKPDVVVAGTQSEDAYS
                     QVKAMVQLGFSPKMLFMSNGANAPTEFPDKVGKANVNGVFSSSTWSPDAKTNGNAEFI
                     AAYQKAYGGSPNGIDGNTADAYAVGQVLQQAVAKIGAIDNQKIIATLHSGTWSTIQGD
                     LQWDADGAPNGKDVLLQWVDGTLVPVFPADRASKQPVAVKPPWGG"
     gene            5105..5980
                     /locus_tag="VF995_07570"
     CDS             5105..5980
                     /locus_tag="VF995_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9343457.1"
                     /translation="MRTVVQAVILGLSAGAVYALMASGLTLAFGVMRVVNVAQGALVI
                     LGAYGSYALFSHLHADPFLSILVVTPLLFVVGVAVQMVFLRRLRSDERELSLLVTFAV
                     AIGIEGVLTLTFKTNYRSINPSYANRTLHLLGYQVPLVRVLAFVLSAGVLALLWLALE
                     RTRFGRALRATVQNPTSARLLGVEVERVAAVGFGLGAATGAAAGAVYGIVWPFNPGSH
                     YDLISRLLSIVVLGGLGSLGGAVVAALLMGVAEAVVAATLSPVWSSFAFFAVLIGVLL
                     VRPQGLFGTVTRGEL"
     gene            5977..7029
                     /locus_tag="VF995_07575"
     CDS             5977..7029
                     /locus_tag="VF995_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9343458.1"
                     /translation="MSWFQPWGVARGLIVFGALALAPLLLPQHWMLNLAIFTLMYAAL
                     AVAWNLIGGYAGYVSLGHAAFFGIGAYTLGITLDKLGLSTPGAASSSFHDLVPFLLLP
                     AIGIAVALMSLPVAWVALRTRATTFAIVTLTLLFVVQTLAFNLTKLTGGSQGLAFPVP
                     SFAVDSFERPFYLAMLALLALAMLISWYVRGAKLGLALFAIRDDEDRARGLGVRTTGA
                     KLSAFAASVGLTAMCGGVWAYYISFIYPQFAVDPLISIGTVLMVYLGGRGTLWGPVLG
                     AAVVIPAQQYLAYRFGQNNLYLIGYAAVFLLVIVLLPRGIAPSLADWFRRRGRRAEAQ
                     EGEPELTPRVPVRGGG"
     gene            7031..7768
                     /locus_tag="VF995_07580"
     CDS             7031..7768
                     /locus_tag="VF995_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9343459.1"
                     /translation="MATDGLLQVEGLTKRFGGVTAVDGCSFAVAEGAVTALIGPNGSG
                     KTTLFNLVTGYLPADQGEVTFLGERVARPHPARLCRQGLARTFQQTRVFPHLSVLDNL
                     VVGIQQPWSGLLRRRRGAADQERAEALVADFGLTAVAGQLAAELSYGQRKLVELATVL
                     IGEPRLVLLDEPTAGISPALIPTIERHIRERNAAGVSFLVVEHDMGVVMRLCDPVIVL
                     DRGSVIASGPPPAIQADPRVLDAYLGG"
     gene            7770..>8359
                     /locus_tag="VF995_07585"
     CDS             7770..>8359
                     /locus_tag="VF995_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9343460.1"
                     /translation="MTAVALLQLRGVVAGYGEAMVLRGVDLDVPKGAVTCVIGPNGAG
                     KSTVLKTVSGLLRPRAGTIVVSGELIGGLTPRAVLGHGVVHLPQDRSLFPAMTVWENL
                     LMGAYIIKDQALVRRRLAEVIELYPLVGQRRGARAGSLSGGEQRIVELARGLLLDPVL
                     VLMDEPSIGLGPQAARQVFDSIAAMPQAGRTVLLVE"
BASE COUNT         1144 a         2975 c         2945 g         1295 t
ORIGIN      
        1 ggttgccgtc gcggcgaagg tcgtagcgga gcggctcagc ggccacggcg cacccccgcc
       61 cggcgccaag cccgctcgcc ctggccgaag ccggccacga tcaccgggac gagggcgacc
      121 agcagcacgc cggcgaggag agcggtggcc agcaggcgga gggcggcggc gatcacgggt
      181 cgccctccag caggtaacgg ttccggccgt cggcggtgaa ggacgccacg ccctcccaga
      241 ccgcatgggc ggcatgccgg agggcggcgt cctggcgcag caccgcggca gcggcaccag
      301 cgacctgcgc gaacgcgatc gaccgggcga ccgcatggcc gctggtcagc tcgtcaagct
      361 ggcagatcgc ctggaggagg ctcttggctg ccgggtcgag cgtggtggtg gcgggtgggg
      421 tagcgttcgg gtcgggcatg aggcggtctc ctcgtgtccc ggcccccgga ggctccggcc
      481 tcgcgggggc actgccaccg ggcaccgatt gatccccggg cccacagccc cgcgtgcttg
      541 tgttgatagt cgcggctgca ccgctcgctt gcgacctgct ggcttggtgg tcagcacagc
      601 gttcagctgg gtgctagcct gcgcctatga ccgctcgtcc tgctcggact cggacgctgc
      661 ctcgtccggt tcgctgcgtg ttcctcgacg ccggtgacac gctgctgtcg ccgtggccga
      721 gcttccacga gcgcttccag caggtcgccc tggaggaggg cgcgagcttc gagctggccg
      781 ccgtcgaggc ggcctgggcg accgcgatcc gcgaggcgct gtggccggag gactggaccg
      841 acccagccac ccaacaggaa ttctggcacg gcttctacgt ggaggtcctg cgccacctcc
      901 ccttcggggg cgaccccgcc gggatcgcca cggccgcctt cgaggtgttc tccgacccgg
      961 ccacctacca gctgttcgac gacagccgcc cggccctgga ggcgatggcc caccgcggcc
     1021 tcaagctcgg ggtgatctcc aacttcgagc cctggctcca ggacatcctg gcgctccaag
     1081 gggtggacca cctgttcgcc gcggtggtga tctcgggggt cgtcgggatc gccaaacccg
     1141 acccggggat cttccacgcc gccctggcgc aggccggggt cgaggcgtcc gaggccgtcc
     1201 atgtcggcga ccagcccgag atcgacgcga aggccgccga ggaggtcggc atcgcgccgg
     1261 tgctgatcga ccgcttcggc cgccggcccg agccggtgcc ctggccccgg atcgcctcgc
     1321 tcaccgagct ggtcgacctg ctcgaccaga agggatagac aggcgatgga tcgcagcttc
     1381 tggcgggtga tcgccaccga cgagaacgcc ctcccgcagg gcgagtgggt cgccgagctc
     1441 accccagagc tgctgagctg gctcagctcc agcgacgcgg agctccgcga tgagttcgcc
     1501 taccggatcc tggccggctg gatcgaccgc gaccagtaca aacccgacca gcttcgcacc
     1561 atggccgcgc agatgaccgc caacctgttc gtcgggctgg gggaggccgg caccgagtcg
     1621 gtgttcctgc gcagctactc ggcgctggtc ctgatggaga tcgtcgccta cgacaacgcc
     1681 aacccgttcc ttgagacctt cgaggtcgaa agctacctcg acgcagcact cgactatctg
     1741 gcccgcgagc gtgacctgcg cgcctacgtg cagggccctg gctgggcgaa cgccatcggc
     1801 cacaccgccg acctgctgat gatgttggcg cgccacccgc accttggcgc tggcgcgctc
     1861 gagcggatcc tggaggggat cgccagcaag ctgctcagcg gcggggcacc tcccttcgtc
     1921 catcacgagg acgagcgcct cgcctacgcc gtgctcaacg tcctgcgtcg cgacctcgtc
     1981 ggctgggtgt tcctgcgcgc ctggatggag cgcttcctcg ccgcaccagg caaggattcc
     2041 tggcgcgcct cgttctccaa cgaggtggag gccgccgccc gcgccaacgt gaccgccttc
     2101 ctgcgcggcc tctacttcca gatcctgttg accgaggatc cgccgccgac cgcgcccgag
     2161 gtcgtcgagg cgatcctcgc caccctcaag ggtatcgaca tcggctacta cgacctcgcc
     2221 tgagctgccg ggttcgccgg gttacctgac ccgttcgatg ctggcgccga ggccgcgcag
     2281 gcgctcgtcg atgcgctcgt agccgcggtc gatctggccg atgttgccga tcaccgactc
     2341 gccgtcggcg accagggcgg ccagcagcag cgccatgccg gcgcggatgt cgggggattc
     2401 caggcgctca ccgaacagcc ggcccgggcc ggtgaccacc gcgcggtgcg ggtcgcagag
     2461 gatgacgcgg gcgcccatgc tgaccagctt gtccacgaag aacaggcggt tctcgtacat
     2521 cttctcgaag atcagcacgg tgccgcggca ctgggtggcg atcgccagcg cgatcgaggt
     2581 gaggtcggcc gggaacgccg gccaggggcc atcatccaac ttcgggatgg ccgcgccgag
     2641 gtcgtcctgg atggtcagct cctgctcgcg tggcacccgg agattcttgc cctcatacag
     2701 ccagcgcacc ccgaggcggt ggaagccgac cgcgaccggg cgcaggtcgt cggcgaccac
     2761 gtcctcgatc accagctcgc cgccgctgat cgcggccagg ccgatgaagc tggcgatctc
     2821 gatgtagtcc ggctggatgc tgtactcgcc gccgctgagc cgctcgaccc cgtcgatgat
     2881 gagcaggttc gagccgcagc cgctgatccg ggccccgcag gcgttgagga accggcagag
     2941 gtcctggatg tgcggctcgc aggcggcgtt gccgatgacc gtctgaccgt cggcgagcac
     3001 cgcggcgctg atcgcgttct cggtcgcggt caccgacgcc tcgtccagga acatcgggtg
     3061 gccgcgcagg cgctcggcct ccagctcaag gacccggtgg tcgaagctgg cacgggcgcc
     3121 gagcgcgcgg aaggcgagca ggtgggtgtc gaggcggcgg cggccgatca cgtcgccgcc
     3181 cggcgacggc agcacgcagc ggccggtgcg tgccagcagc ggcgcggcca gcagcaccga
     3241 ggcgcggatc tggctgcaca gcagcgggtc gaggctggta cggcgcacgc cgcgcgggtc
     3301 gatccggacc gagttcgggc cgacgtcgct cacctcggcg ccggtcgagg cgaccagctg
     3361 cagcagagtg gccacgtccc ggatccgcgg aacgttgtgg agcacgaccg gcccgtcgca
     3421 cagcagcgtc gccgagataa tcggcagcgc ggcgttcttg ttcccagcgg ggcggatcga
     3481 gccgcagagg ggctgcccac ccttgatcac gaagctttcc aatcctctcc cgctcctgct
     3541 cactcgacgc tggggacagg tcgcctactt taccgtcccg atgctccaaa gcggcgcacc
     3601 tgcacatcgc agttcggtga acgccgctcg tttccccaga ctcagccagt attgccggta
     3661 attgcctccc gtatgtgtcg gcgggaggtc tgaccggttg acaacggcag cgggatcagc
     3721 ggagagtcag cccgtatgca aacgtttgcc atgctcccgc aggaggcgat catgggcaag
     3781 ccgcacgggc acggacggcc gtacggacgg ccgcagggac ggttcaggtt cgccgcggcc
     3841 ctcgccgtcg cggcgctgct gctcgccgcc tgctcatcgg ggaagtcgtc ctccccgtca
     3901 ggtggcaacc aggcccagaa cagcaagccg atccggatcg gggcatccat ctcgcgctcg
     3961 ggggacttct ccgacccggc ccagctggtc ctccggggct acaaactgtg ggccgaccag
     4021 gtgaacgcca agggcggcct gctcggccgc aaggtcgaga tcgtgaccgt ggacgacgcc
     4081 tccagcccca accaggcggt caccaactac cagaacctga tcacccgcga caaggtggac
     4141 ctgaccttcg ggccgttctc gagcctgctg acgctcccct cggcccaggt cgccaagcgc
     4201 tacggctacg ccttcctgga gcccgagggc ggcggtccca aggtgttcgc ggccaagctg
     4261 ccgaacctgt tcttcgtgca gcccgcccca gcggtcgccc agggccaggt gttcgccgac
     4321 tacgtcctgt cactgcccgc ggcgcagcgc ccgaagaccg ccgcctaccc gtcgttggac
     4381 gacccgttcg cgtcgccggt cgcggaagcc gtccggaccc gcttcgagca ggccggcatc
     4441 aggaccgtct acaaccacat ctacccggcc gagaccaccg acctgacccc gatcgtgcag
     4501 aaggtcgccg cggcgaagcc ggacgtggtc gtcgccggga cccagtccga ggacgcctac
     4561 tcgcaggtca aggcgatggt ccagctcggc ttcagcccca agatgctgtt catgtccaac
     4621 ggcgccaacg cccccacgga gttccccgac aaggtcggca aggccaacgt caacggggtc
     4681 ttctcctcaa gcacctggtc gccggacgcc aagaccaacg gcaacgccga gttcatcgcc
     4741 gcctaccaga aggcctacgg cggcagcccg aacgggatcg acggcaacac cgccgacgcc
     4801 tacgccgtcg gccaggtgct gcagcaggcg gtggccaaga tcggcgcgat cgacaaccag
     4861 aagatcatcg ccacgctgca cagcggaacc tggtcgacca tccagggcga cctgcagtgg
     4921 gacgccgacg gcgcgcccaa cggcaaggac gtgctgttgc agtgggtcga cgggacgctc
     4981 gtcccggtct ttccggcgga cagggcgtcc aagcagccgg ttgcggtgaa gccgccgtgg
     5041 ggagggtagg taggcagcgg acctgaagcc ctgaactggc ggcggagctg aaggggaggg
     5101 atcggtgcgc acggtggtcc aggccgtcat cctcggcctg tcggcgggag cggtgtacgc
     5161 gctgatggcc agcggcctca cgctggcgtt cggggtcatg cgggtggtga acgtggcgca
     5221 gggggcgctg gtgatcctcg gcgcctacgg cagctatgcg ctgttcagcc acctgcacgc
     5281 cgaccccttc ctctcgatcc tggtggtcac gccgctcctg ttcgtggtcg gcgtggcggt
     5341 gcagatggtc ttcctgcggc ggctgcgcag cgacgagcgg gagctgtcgc tgctggtcac
     5401 cttcgcggtc gcgatcggca tcgagggggt gctgaccctc accttcaaga ccaactaccg
     5461 ctccatcaac cccagctacg ccaaccgcac gctgcacctg ctcggctacc aggtgccgct
     5521 ggtgcgggtg ctcgccttcg tcctgtcggc cggggtgctg gcgctgctgt ggctggcgct
     5581 ggagcgcacc cgtttcggcc gggcgctgcg cgccacggtg cagaacccga cctcggcgcg
     5641 gctgctcggc gtcgaggtcg agcgcgtcgc cgcggtcggg ttcgggcttg gcgccgccac
     5701 cggcgccgcc gccggggcgg tctacggcat cgtctggccc ttcaacccgg gcagccacta
     5761 cgacctgatc tcgcggctgc tgtcgatcgt ggtccttggc gggctcggca gcctcggcgg
     5821 cgcggtggtc gccgcgctgc tgatgggcgt ggcggaggcg gtggtcgcgg ccacgctgtc
     5881 gccggtgtgg tcgtcgttcg cgttcttcgc ggtgctcatc ggcgtcctgc tggtgcgacc
     5941 ccaggggctg ttcggcaccg tcacgcgggg cgagctgtga gctggtttca gccgtggggg
     6001 gtcgcccggg gcctgatcgt gtttggcgcg ctcgcgctgg cgccgctgct gctgccgcag
     6061 cactggatgc tgaacctggc gatcttcacg ctgatgtacg ccgcgctggc ggtggcgtgg
     6121 aacctgatcg gcggctacgc cggctatgtc tccctcggcc atgccgcctt cttcgggatc
     6181 ggcgcctaca ccctggggat caccctggac aagctcgggc tcagcacgcc cggggcggcg
     6241 tcctccagct tccacgacct ggtcccgttc ctgctgctgc ccgcgatcgg gatcgccgtc
     6301 gcgctgatga gcctgccggt cgcctgggtg gcgctgcgga cccgggcgac cacgttcgcg
     6361 atcgtcaccc tgacgctgct gttcgtggtg cagaccctgg ccttcaacct gaccaagctg
     6421 accggcgggt cgcaggggct cgcgttcccg gtgccatcgt ttgcggtgga cagcttcgag
     6481 cggcccttct acctggcgat gctcgccctg ctcgccctcg cgatgctgat cagctggtat
     6541 gtccgcgggg cgaagctcgg gctcgcgctg ttcgccatcc gcgacgacga ggaccgcgcc
     6601 cgcggccttg gtgtgcgcac caccggcgcg aagctcagcg ccttcgcggc cagcgtcggc
     6661 ctgaccgcga tgtgcggcgg ggtgtgggcc tactacatct ccttcatcta cccgcagttc
     6721 gcggtcgatc cgctgatctc gatcggcacg gtgctgatgg tctacctggg cggccggggc
     6781 accctctggg gcccggtgct gggcgcggcg gtggtcatcc cggcccagca gtacctcgcc
     6841 taccgcttcg gccagaacaa cctctacctg atcggctacg cggcggtgtt cctgctggtc
     6901 atcgtcctgc tcccacgcgg gatcgcaccg tcgctcgccg actggttccg ccgccggggc
     6961 cggcgggccg aggcccagga gggggagccg gagctgaccc cccgcgtccc cgtccgggga
     7021 ggtggctgag gtggccacgg acggcctgct ccaggttgag ggcctgacca agcggttcgg
     7081 cggggtcacc gccgtcgacg gctgctcgtt cgccgtcgcc gagggggccg tgaccgccct
     7141 gatcgggccc aacgggtccg gcaagaccac cctgttcaac ctggtcaccg gctacctgcc
     7201 cgccgaccag ggcgaggtca ccttcctggg agagcgggtg gcgcgcccgc acccggcgcg
     7261 gctctgccgg caggggctgg cgcggacctt ccagcagacc cgggtcttcc cgcacctgag
     7321 cgtgctcgac aacctggtgg tcgggatcca gcagccctgg agcgggctgc tgcgccgccg
     7381 ccgcggcgcc gccgaccagg agcgggccga agcgctggtg gccgacttcg gcctcaccgc
     7441 cgtcgctggg cagctcgccg ccgagctgtc ctacggccag cgcaagctgg tcgagttggc
     7501 gacggtgctg atcggcgagc cgcgcctggt cctgctcgat gagccgaccg ccgggatcag
     7561 ccccgccctc atccccacga tcgaacggca catccgcgag cgcaacgccg ccggggtcag
     7621 cttcctggtg gtcgagcacg acatgggtgt ggtgatgcgg ctgtgcgacc cggtgatcgt
     7681 gctcgaccgc ggcagcgtca tcgccagcgg cccgccgccc gcgatccagg ccgacccgcg
     7741 cgtcctcgac gcctacctgg gaggctgacg tgacggctgt ggcactgctg caactgcgcg
     7801 gggtggtggc cggctacggt gaggcgatgg tgctgcgcgg cgtcgacctc gacgtcccca
     7861 agggcgcggt gacctgtgtc atcggcccca acggggccgg caagtcgacc gtgctgaaga
     7921 ccgtcagcgg gctgctgcgg ccccgcgccg gcacgatcgt cgtcagcggt gagctgatcg
     7981 gcgggctgac cccccgggcc gtcctcggcc acggcgtggt gcacctgccg caggaccgga
     8041 gcttgttccc ggcgatgacg gtgtgggaga acctgctgat gggtgcctac atcatcaagg
     8101 accaggcgct cgtccggcgg cgcctggccg aggtcatcga gctgtatccc ctggtcggcc
     8161 agcggcgggg tgcccgcgcc ggttcgctgt ccggcggcga gcagcggatc gtcgagctgg
     8221 cccgggggct gctgctcgac ccagtgctgg tgctcatgga cgagccctcg atcgggctcg
     8281 gcccgcaggc cgcccgccag gtcttcgaca gcatcgcggc gatgccgcag gcgggccgca
     8341 ccgtcctgct ggtggagca
//
LOCUS       DASWDC010000234        10185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1394184, whole genome shotgun sequence.
ACCESSION   DASWDC010000234 DASWDC010000000
VERSION     DASWDC010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10185
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394184"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..982
                     /locus_tag="VF995_07590"
     CDS             122..982
                     /locus_tag="VF995_07590"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9343461.1"
                     /translation="MDEAEAGTAREHLAANRALWDEWTGVHLRSEFYDVAGWKAGRRP
                     MRAFLLDELGDVNGKDVLHLQCHFGLDTLALARLGARVTGVDFSERAVEAARGLAVEV
                     GLGDRSRFVCSDVLELPAALTGDFDVVFTTIGVLSWLPDLARWARVVARYLRPGGFVY
                     VADAHPFAQVYDDEADELRLRYPYWESADPIAWKVQGSYADPDAVVEQEVEYAWTHSM
                     GEIVTVLADAGLRIEFLHEFPFVVWKMLPFMVEHPTLEGETEWRLPGGQQQFLPLLFS
                     LKASKPAAAG"
     gene            complement(1117..1353)
                     /locus_tag="VF995_07595"
     CDS             complement(1117..1353)
                     /locus_tag="VF995_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343462.1"
                     /translation="MYEPFAANIARRLTENLATSALPDAPVRTEPEPATRRRAVAPHL
                     RERSARLLVSLAARLDPATPKPAMLSTARSRPAR"
     gene            1376..1618
                     /locus_tag="VF995_07600"
                     /pseudo
     CDS             1376..1618
                     /locus_tag="VF995_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
     gene            complement(1636..2790)
                     /locus_tag="VF995_07605"
     CDS             complement(1636..2790)
                     /locus_tag="VF995_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9343463.1"
                     /translation="MADVLLEDVSRIHADGTVAVDHLSLHVRDHELLVLLGPSGCGKS
                     TTLRLIAGIEPLSGGSITIGGRIVTNLAPHERDVAMVFETNSVYPHLTGGENLRFGLA
                     LRGLPEAEIDQRVQAEARVLRIRHLLGRLPKTMSAGQRQRLGLGRATVRVPTVFLLDE
                     PLTHLDETERVRLRTELNRILHGLGVTTLYVTHDQSQAMAIGDRVAVMRAGRLEQVGT
                     PRALYQEPANTFVATFLGSPAMNLLTGRIELEGVPGSAWIALGARDREQRLRFPGVPS
                     GPLRGYTGSGEPLTVGIRPEHLSAAGTATPPERRLAVTARRVDRLGPELLATCELPGP
                     SAGGGAAETLAARLAARHPIRAGEQLDLAVEVGRLSYFDPRSGAALWHGS"
     gene            2881..4182
                     /locus_tag="VF995_07610"
     CDS             2881..4182
                     /locus_tag="VF995_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9343464.1"
                     /translation="MAMSMAEQARGLTREQRNAFVASFLGWSMDAFDYFLIIFVFTEI
                     SKDLHSSLIKVQFATTLTLAARPVGAAIFGLLADRFGRRVPLMVDVAFYSVIEFLTGF
                     SSSITMLLFLRTLYGIGMGGEWGIGASLAMEKIPREKRGFWSGLLQQGYPFGYLLAAV
                     AYFLIGKNFGWRWLFWAGSIPALLVLFIRARVGESEVWERARQRESTIGSILRQPAVI
                     KRFLFLLVLMTLFNFMSHGTQDQYPTFLKTQHHYSADLAVLVAVIYNLGALCGGITIG
                     ALSESIGRRKAVVWVTLLALPVVPLFAFSSVLGLIVLGSFIMQFLVQGAWGIIPAHLS
                     ELSPDEIRGFYPGVTYQLGNLLAAINLPLQTRLADHYNKNFGLGMALVVAPVLLLTAL
                     VTWLGPENRGAELGRIATPGREEVGARAGPRGPGPGVPAEG"
     gene            4285..4506
                     /locus_tag="VF995_07615"
     CDS             4285..4506
                     /locus_tag="VF995_07615"
                     /inference="COORDINATES: protein motif:HMM:NF020512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HEX9343465.1"
                     /translation="MVNIGDRVRIESQRVGSVTRIGTVTGIEGRMITVRWDSGSQSMF
                     VPSAGCLEVIGHESVPVGPPQAQARAERD"
     gene            complement(4503..5798)
                     /gene="lysA"
                     /locus_tag="VF995_07620"
     CDS             complement(4503..5798)
                     /gene="lysA"
                     /locus_tag="VF995_07620"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX9343466.1"
                     /translation="MTGHQPPHPFRYHGGILHVGGLPLDQVVAATGTPAYVYDLDAVA
                     AAYQRVASAFAPIGGRVLFAVKANSSLAVLARLAQLGSGFDVVSGGELLRVLRSGGDP
                     AATVFAGVGKTASELDLAVAHGVTVHVESADELNALEAAAARGGTTIPFALRVNPDVE
                     VDTHAHIQTGHDEAKFGVPLEVAEDLLGRTAKGDHPHLRAVGIHVHVGSQLPDPDGLV
                     DAARTGLAMIEAGRAAGLELHTLDVGGGLPISYNGDQIDSRGAPLPGPEDFAHALAPV
                     VAGRDVTLLIEPGRYLVGPAGALVASVLYRKHRSAGRMLVVDTGMHQLMRPALYQAVH
                     RVVPLRQPPSSSSDAATEPTEVVGPICESTDVLAAAADLPELAPGDLVAFLDTGAYGM
                     TMASNYNSHPRPPEIVVEGGTARVARRRETWDDLLSFED"
     gene            5971..7020
                     /gene="argC"
                     /locus_tag="VF995_07625"
     CDS             5971..7020
                     /gene="argC"
                     /locus_tag="VF995_07625"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX9343467.1"
                     /translation="MSATVGILGGSGYAGGELLRLLAGHPHLRVVWAAADSAAGRPVT
                     DRYPGLASAYGALTWCTVDEGLGKGADVLFLALPHGQAAQLVARVLAAAGLVVDLSAD
                     FRLRDADAYPAWYGAAHPAPQQLGGWPYGLPELHRDELRGATRIAVPGCYPTAALLAL
                     APALAGGLVRPDGIGIDAKSGLSGAGRSLKDPNLFVQANENVTPYGIGTHRHTPEIEQ
                     ELSRLAGRPLQVSFTPHLVPQNRGILATCYATLTDPDLAAPCGVASCYQAAYGDEPFI
                     DLLGEGRWPGTRAVATTNRVQVAVTADPRTNRLVAAAAIDNLVKGAAGQALQCANLAL
                     GLPETAGLDLVPPGP"
     gene            7055..8338
                     /gene="argJ"
                     /locus_tag="VF995_07630"
     CDS             7055..8338
                     /gene="argJ"
                     /locus_tag="VF995_07630"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX9343468.1"
                     /translation="MTPATGRFQVDRGLGVCAPAGFSAAGVACGIKESGGLDLALVVG
                     APGTVAAGVFTTNLVVAAPVVWTRELLAASSDARVVVLNSGNANACTGAAGDAAVLAS
                     AQRAAVELGCQPEQVLICSTGVIGVPVPTDALTDGVAKAAIGLDPGPAGATAAAEAIR
                     TTDTVAKQAGTRLGPVTIGGMAKGAAMLAPALALLGPAHATMLAVVTTDAVAEPATLR
                     AALAAATATTFNRITVDGAQSTNDTVLLLASGTSRVAPAPEELAAAVEAVCDDLAGQM
                     LADAEGGTRVVAVHVGGASTAEAALTVARRVADSPLVKTAVFGGDPNWGRILQAAGTA
                     GVPFDPARVRLQVRGGPGAAGLQPEPEPGRPGAVSDPVELIRDGAPTGADGSAPMAER
                     EIEFRLDLGGPGPWACVRTADLTPEYVRLNSEYTT"
     gene            8335..9243
                     /gene="argB"
                     /locus_tag="VF995_07635"
     CDS             8335..9243
                     /gene="argB"
                     /locus_tag="VF995_07635"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX9343469.1"
                     /translation="MNTATEDLQAKADVLHEALPWIKRFAGRTAVIKYGGAAMADAGS
                     FVDDVVLLRYVGVDVVVVHGGGPEVSHAMRELGQTPVFVDGHRVTDADTIDLVRMILV
                     GRINKGLVGRFNRHGRIAVGLSGEDGLLVSATPRHDPGGRDLGFVGDVASVDTTALRA
                     LTGAGLIPVIATVAAGPEGRPYNVNADAVAGALAAALGAEKLVYLTDVEGLYRDVADP
                     ASLLRHLTLAEVEALLESDQAHSGMVPKLRGIVTALGAGVGSAHILDGRLRHALLLEL
                     FTNKGVGTMVGPNGWNDLDTVREGVA"
     gene            9240..>10185
                     /locus_tag="VF995_07640"
     CDS             9240..>10185
                     /locus_tag="VF995_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007380784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9343470.1"
                     /translation="MTIAEREAAVLWPTYARPEIEFVRGEGVHLWDAEGREYLDFLGG
                     IAVVAAGHANPKVVAAVAGQLATLGHTSNLYFTGPQVRLAERLVAAFGGDGKVFFSNS
                     GAEAIEAAIKVARRWGRANRGERAIGIVCTDGAFHGRTLATLAATGKPAIKAAFEPLP
                     AGFSHVPFGDADALAAAIGPDTAAVLLEPVQGENGIRVPPAGYLRAARELTADAGVLL
                     ILDEIQSGMGRTGRFFAHQHHGVRPDVVTMAKALGSGYPIGATIATNEVADTMHPGDH
                     GTTFGGNPVACAAALATLDVIEPLLDGHVDTVAGRLRDG"
BASE COUNT         1327 a         3497 c         3840 g         1521 t
ORIGIN      
        1 ggccgaacgt aggccccccg atgcgccgaa cgtaaccgaa gcaaggatcc aggtggaggg
       61 tgcggccgaa attgtggaaa acccggtgcg gtcacccccg ggcgggtggg aggcttggat
      121 catggacgag gcggaggcgg ggacggcgcg ggagcatctc gcggcgaacc gggcgctgtg
      181 ggacgagtgg acgggggtgc atctccgctc ggagttctat gacgtggcgg ggtggaaggc
      241 gggacgcagg ccgatgcggg cgttcctgct ggatgagctt ggcgacgtga acggcaagga
      301 cgtgctgcac ctgcagtgcc atttcgggct tgacacgctt gcgttggcgc ggttgggggc
      361 gcgggtgacc ggggtggatt tctctgagcg ggcggtcgag gcggcgcggg ggctggccgt
      421 tgaggtcggg ctcggcgacc ggtcgcggtt tgtctgctct gacgtgctgg aattgcccgc
      481 ggcgctgacc ggggacttcg acgtggtgtt caccaccatt ggggtgttga gctggctgcc
      541 ggacctggcc cggtgggcgc gggtggtggc gcgctatctg cggccggggg gtttcgtgta
      601 tgtcgctgat gcgcatccgt tcgcgcaggt ctacgacgac gaggccgacg agttgcgtct
      661 ccgctacccg tactgggagt cggccgaccc gatcgcctgg aaggtgcaag gttcctacgc
      721 ggacccggac gcggtcgtcg agcaggaggt cgagtacgcc tggacgcact ccatgggcga
      781 gatcgtcacg gtgctcgccg acgccgggct gcgcatcgag ttcctgcacg agttcccgtt
      841 tgtggtgtgg aagatgttgc cgttcatggt cgagcacccg accttggagg gtgagacgga
      901 gtggcgcctc cccggcggcc agcagcagtt cctgccgctg ctgttctcgc tgaaggccag
      961 caagcccgcg gcggccggct gaactggttg gtgatcgggg aggcgcggcg cgttactctg
     1021 gtggtcgctc ggtgacgcgg ggggacgtgg gatctttgcc cttgaccgaa ctccatgggg
     1081 aggttcggac cctggctccc atcgatgcgc ccccgctcac cgagcgggtc ggctcctggc
     1141 ggtggagagc atcgcgggct tgggggtggc cgggtccagc cgggcggcga ggctgaccag
     1201 caggcgcgct gaccgctcgc gcaggtgcgg tgcgacggct cggcggcgcg tcgctggttc
     1261 gggttcggtg cggacgggcg cgtccggcag cgccgaggtg gcgagattct cggtcaggcg
     1321 gcgagcgata ttggctgcga acggctcata catggcggcc tccgtgtccc ctgtcctctg
     1381 cttgtcacct ccaccggtcg ccgagcgcgt acggcggctg gaggagcagg gcgtcatcgc
     1441 cggctatcac gcccgggtcg acccggcccg cgccgggctt ccattgacag ccttcgtcca
     1501 gattcgatcc cggcttgacc attgcctgct gcaaaccagc aaatccagcg actatcccga
     1561 ggttgtggag gtccacaagc tcagcggcga ccactgctcc gtcctcaagg tccgtgccgc
     1621 ccaggggtgg agcggctaac tgccgtgcca gagggccgcg ccggagcgtg ggtcgaagta
     1681 ggagaggcgg ccgacctcca cggccaggtc gagctgctcg cccgcgcgga tcgggtggcg
     1741 ggcggccagc cgtgcggcga gggtctccgc cgctccgcct ccagccgaag gcccgggaag
     1801 ttcgcaggtg gccagcagtt ccgggccgag gcggtcgacg cggcgggcgg tgacggccag
     1861 gcgccgctcc ggcggggtcg cggtcccggc ggcgctcagg tgctctggcc ggatgccgac
     1921 ggtgagcggc tcgccgctgc cggtgtagcc gcggagcggg ccggagggga cgccggggaa
     1981 gcggagccgc tgctcccggt cccgggcgcc gagggcgatc caggcgctgc ccggcacgcc
     2041 ctccagctcg atccgtccgg tcagcaggtt catcgccggg gagccgagga aggttgccac
     2101 gaaggtgttc gcgggttcct ggtagagggc gcggggggtg ccgacctgct ccaggcggcc
     2161 ggcgcgcatc acggcgaccc ggtcgccgat cgccatcgcc tgggactggt cgtgggtgac
     2221 atagagggtg gtcaccccga ggccgtgcag gatccggttc agctcggtgc gcagccgcac
     2281 ccgttcggtc tcgtcgaggt gggtgagcgg ctcgtccagc aggaacaccg tggggacccg
     2341 gacggtggcg cggccgaggc cgagtcgctg gcgctgcccg gcggacatgg ttttggggag
     2401 ccgcccgagc aggtggcgga tgcgcaggac tctggcctcg gcctggacgc gctggtcaat
     2461 ctcggcctcg ggcaggccgc gcagagccag gccgaagcgc aggttctcgc cgccggtcag
     2521 gtgcgggtag accgagttgg tctcgaagac catggccacg tcgcgttcgt ggggtgcgag
     2581 gttggtgacg atccgcccgc cgatggtgat cgacccgccg ctgagcggct cgatcccggc
     2641 gatcagccgc agcgtggtgc tcttgccgca gcctgacggg ccgagcagca ccagcagctc
     2701 atggtcgcgg acgtggaggc tgaggtggtc gaccgccacg gtgccgtccg cgtggatccg
     2761 tgagacgtct tcgagcagca cgtccgccat ggcggtgctc ctcagctcag gttaccgacc
     2821 agaggcctgt gggtacgcaa gcccgcgcgg gaggggaccg gaccgcaggt ggtggctggg
     2881 atggcaatga gcatggccga gcaggcccgc gggctcacgc gcgagcagcg caacgccttc
     2941 gtcgcgagct tcctcggctg gtcgatggac gcgttcgatt acttcctcat catcttcgtc
     3001 ttcaccgaga tcagtaagga cctgcactcc tcgctgatca aggtgcagtt cgccaccacg
     3061 ctgacgctgg cggcgcggcc ggtcggcgcg gcgatcttcg ggctgctggc cgaccggttc
     3121 gggcggaggg tgccgctgat ggtggacgtg gcgttctact cggtgatcga gttcctcacc
     3181 gggttctcgt cgtcgatcac catgctgctg ttcctgcgga cgttgtacgg gatcggcatg
     3241 ggtggcgagt gggggatcgg cgcgtcgctg gcgatggaga agatcccccg ggagaagcgc
     3301 gggttctggt cggggctgct gcagcagggc tacccgttcg ggtacctgct ggccgcggtc
     3361 gcgtacttcc tgatcgggaa gaatttcggc tggcgctggc tgttctgggc cgggtcgatc
     3421 ccggcgctgc tggtgctgtt catccgtgcg cgggtggggg agtccgaggt ctgggaacgg
     3481 gcccggcagc gggagagcac catcgggtcg atcctccggc agccggcggt gatcaagcgg
     3541 ttcctgttcc tgctggtgct gatgacgctg ttcaatttca tgagccacgg gacccaggac
     3601 cagtacccga cgttcctgaa gacgcagcat cactactccg ccgacctggc ggtgctggtg
     3661 gcggtgatct acaacctggg cgcgctgtgc ggcgggatca ccatcggggc gctgtcggag
     3721 tcgatcgggc ggcgcaaggc ggtcgtgtgg gtgacgctgc tggcgttgcc ggtggtgccg
     3781 ctgttcgcgt tctcgtcggt gctcgggctg atcgtgcttg gctcgttcat catgcagttc
     3841 ctggttcagg gggcttgggg gatcatcccg gcgcatctgt cggagctgtc gccggacgag
     3901 atccgcgggt tctatccggg ggtgacctat cagctcggga acttgctggc ggccatcaac
     3961 ctgccgctgc agacccgcct cgccgaccac tacaacaaga acttcggcct ggggatggcg
     4021 ctggtggtcg cgccggtgct gctgctgacc gccctggtca cctggctcgg cccggagaat
     4081 cgtggggcgg agctgggccg catcgccact ccgggccgcg aggaggtggg cgcccgcgcg
     4141 ggcccgcgcg gacctgggcc gggtgtgccc gccgaggggt agcggcggcg ggcacaggcc
     4201 ccttgcccgt tcggggttgg ggcgtcacac tcgattgtcg tatttctccc gcttcctgat
     4261 tgattcgagt gctgggggtc agcgatggtc aacatcggcg atcgtgtccg gatcgagtcc
     4321 cagagagtgg gcagcgtgac ccggatcggc acggtgaccg ggatcgaagg ccgcatgatc
     4381 accgtccgct gggactcggg cagccagtcg atgttcgtgc cgagcgccgg ctgcctggag
     4441 gtcatcggcc acgagagcgt gcccgtggga ccgccgcagg cgcaggcgcg ggccgagcgc
     4501 gactaatcct cgaagctgag caggtcgtcc caggtctcgc ggcggcgggc gactctggcg
     4561 gtgccaccct cgaccacgat ttcggggggg cggggatggc tgttgtagtt cgacgccatg
     4621 gtcatgccgt aggcgccggt gtcgaggaag gcgaccaggt ccccgggcgc cagctcgggg
     4681 aggtcggcgg ccgcggcgag gacgtcggtg gactcgcaga tcgggccgac gacctcagtc
     4741 ggctccgtgg ctgcgtcgct ggagctggag ggcggctggc ggagcgggac gacccggtgg
     4801 accgcctggt agagggcggg gcgcatcagc tggtgcatgc cggtgtcgac caccagcatg
     4861 cggcccgcgc ttctgtgctt gcggtagagg acgctggcga ccagcgcgcc ggccgggccg
     4921 acgaggtagc ggcccggctc gatgaggagg gtgacgtcgc ggccggcgac gaccggggcg
     4981 agcgcgtggg cgaagtcctc cgggccgggg agcggggcgc cccggctgtc gatctggtcg
     5041 ccgttgtagc tgatcgggag accgccgccg acgtcgaggg tgtgcagctc cagcccggcg
     5101 gcgcggccgg cctcgatcat ggcgaggccg gtgcgggcgg cgtcgacgag gccgtcgggg
     5161 tcggggagct gggagccgac gtggacatgg atgcccaccg ctcgcaggtg gggatggtcg
     5221 cccttggcgg tgcggccgag caggtcctcg gcgacttcca gggggacgcc gaacttggcc
     5281 tcgtcgtggc cggtctggat gtgggcgtgg gtgtcgacct cgacgtcggg gttgacgcgg
     5341 agggcgaagg ggatggtggt gccgccgcgg gcggcggctg cttccagggc gttcagctcg
     5401 tcggcggact cgacgtggac ggtgacgcca tgcgcgacgg cgaggtccag ctcgctggcg
     5461 gtcttgccga cgccggcgaa gacggtcgcg gccgggtcgc cgccgctgcg cagcacccgc
     5521 agcagctcgc cgccgctgac cacgtcgaag ccgctgccga gctgggcgag ccgggcgagc
     5581 acggcgaggc tggagttggc cttgaccgcg aacaggaccc ggccgccgat cggggcgaag
     5641 gcgctggcga cccgctggta ggcggccgcc accgcgtcga ggtcgtagac gtaggcgggc
     5701 gtgccggtgg cggcgaccac ctggtcgagc gggaggccgc cgacgtgcag gatcccgccg
     5761 tggtagcgga acgggtgggg gggttggtga ccggtcatag gcgtgaggat aggtcaggtc
     5821 gggccccgcg gtcgtgcgcg ctgggccgga ggacgtcgac ggcgagcgtt gcatggaagc
     5881 gccgatctgg caactcttta ttccagcagc ataggaggat cacgcagaag tggtgttgca
     5941 tattttcatc aagcagtgaa tagttatgcc atgagcgcga cggtgggcat cctgggcggc
     6001 tccggctacg ccggaggcga gctgctccgc ctgctcgccg ggcacccgca cctccgggtc
     6061 gtctgggcgg cggcggactc ggcggctggc cggcctgtga ccgaccgcta tcccggtctc
     6121 gcctctgcct atggggcgct cacctggtgc accgtcgacg agggtctcgg caagggcgct
     6181 gacgtcctct tcctggccct gccccacggg caggcggcgc agttggtggc gcgggtgctg
     6241 gccgccgccg ggctggtggt ggacctgtcg gcggacttcc ggctccgcga cgccgacgcc
     6301 taccccgcct ggtacggcgc ggcccacccc gcgccgcagc agctcggcgg ctggccctac
     6361 ggcctgcccg agctgcaccg cgacgagctg cgcggcgcca ccaggatcgc ggtgcccggc
     6421 tgctacccga ccgcggcgct gctggcgctc gccccggcgc tggcgggcgg gctggtccgc
     6481 cccgacggca tcgggatcga cgccaagtcg gggctcagcg gcgccggccg ttcgctcaag
     6541 gaccccaacc tgttcgtcca ggccaacgag aacgtcactc cctacggcat cggtacccac
     6601 cgccacacgc ccgagatcga gcaggagctg agccgcctcg ctgggcggcc gctccaggtc
     6661 agcttcaccc cgcacctggt gccgcagaat cggggcatcc tcgctacctg ctacgccacc
     6721 ctcaccgacc cggacctggc cgcgccctgc ggcgtggcct cctgctacca ggccgcctac
     6781 ggcgacgaac cgttcatcga cctgctcggc gagggtcgct ggcccggcac ccgcgcagtc
     6841 gccaccacca accgggtcca ggtcgcggtc accgccgacc cgcgcaccaa ccggctggtc
     6901 gccgccgccg cgatcgacaa cctcgtcaag ggcgccgccg gccaggcgtt gcagtgcgcc
     6961 aacctcgccc tcggcctgcc ggagacggcc ggcctcgacc tggtcccccc gggaccgtga
     7021 ggcggtgaca acccccatga cctcgatgac ccccatgact cccgcgaccg gccggttcca
     7081 ggtggaccgc ggccttggcg tctgcgcccc cgccgggttc agcgccgccg gggtcgcctg
     7141 tgggatcaag gagtccggcg gcctcgacct cgccctggtc gtcggcgcgc cgggcacggt
     7201 cgcggcgggg gtcttcacca ccaacctggt ggtggccgcg ccggtggtgt ggacgcggga
     7261 actgctcgcc gcctcgtccg acgcccgggt cgtggtgctc aactccggca acgccaacgc
     7321 ctgcacgggc gctgcgggcg acgcggcggt gctcgccagc gcgcaacggg ccgccgttga
     7381 gctgggctgc cagcccgagc aggtgctgat ctgctccacc ggcgtgatcg gcgtcccggt
     7441 cccgaccgac gccctcaccg acggggtcgc caaggcggcg atcggcctcg accccggtcc
     7501 ggcgggcgcc accgccgcag ccgaggcgat ccgcaccacc gacacggtcg ccaagcaggc
     7561 cggcacccgc ctcggcccag tcaccatcgg cggcatggcc aagggtgccg ccatgctcgc
     7621 cccggcgctg gcccttctcg gtccggcgca cgcgaccatg ctggcggtgg tcaccaccga
     7681 cgcggtcgcc gaaccggcga cgctgcgggc cgccctggcc gcggccaccg cgaccacctt
     7741 caaccggatc accgtggacg gcgcccagtc caccaacgac accgtgctgc tgctcgcttc
     7801 cggcacctcc agggtggcgc ccgcgcctga ggagctggcc gccgcggtcg aggcggtctg
     7861 cgacgacctg gccgggcaga tgctggccga cgccgagggc ggcacccggg tcgtcgccgt
     7921 ccacgtcggc ggcgccagca ccgcggaggc ggcgctgacg gtggcccggc gggtggccga
     7981 cagcccgctg gtcaagaccg ccgtcttcgg cggcgacccg aactggggcc gcatcctcca
     8041 ggccgccggc accgccgggg tgccgttcga ccccgcgcgg gtacgcctcc aggtccgcgg
     8101 cggtccggga gcggccgggc tccaaccaga accggagccg gggcggccgg gagcggtgtc
     8161 ggatccggtc gagctgatcc gggacggcgc cccgactggc gccgacggca gcgcacccat
     8221 ggccgaacgg gagatcgagt tccgcctgga tctcggcggc ccaggcccct gggcttgcgt
     8281 gcggaccgcc gacctgaccc ctgagtacgt ccgcctcaac tcggaataca caacatgaac
     8341 accgcgacgg aagacctcca ggcgaaagcg gacgtgctcc acgaggcgct gccgtggatc
     8401 aagcgcttcg ccggccgcac cgcggtcatc aagtacggcg gcgcggccat ggcggacgcc
     8461 ggctccttcg tcgacgacgt ggtcctgctc cgctacgtcg gcgttgacgt ggtggtggtc
     8521 cacggcggcg ggcccgaggt cagccacgcc atgcgggaac tgggccagac gccggtgttc
     8581 gtcgacggcc accgggtcac tgacgccgac accatcgacc tggtccgcat gatcctggtc
     8641 gggcgcatca acaagggcct ggtggggcgc ttcaaccgcc acgggcgcat cgcggtcggg
     8701 ctctccggcg aggacggcct gctggtcagc gcgacgccgc ggcacgaccc gggcgggcgc
     8761 gatctcggct tcgtcggcga cgtcgcctcg gtcgacacca ccgccctgcg ggcgctcacc
     8821 ggtgccgggc tgatcccggt gatcgccacg gtcgccgccg gcccggaggg gcggccctac
     8881 aacgtcaacg ccgacgcggt cgccggggcg ctcgccgccg cgctcggcgc cgagaagctc
     8941 gtctacctca ccgacgtgga gggtctctac cgcgacgtcg ccgacccggc ctcgctgctg
     9001 cgccacctca ctctggctga ggtcgaggcg ctgctggaaa gcgaccaggc gcacagcggc
     9061 atggtcccca agctccgcgg catcgtcacc gccctcggtg ccggcgtcgg cagcgcccac
     9121 atcctcgacg ggcgcctgcg ccacgccctg ctgctggagc tgttcaccaa caagggcgtc
     9181 ggcaccatgg tcggccccaa cggctggaac gacctcgaca ccgtgagaga aggagtcgca
     9241 tgacgatcgc ggagcgggag gcagcggtgc tgtggccgac ctatgcgcgg cccgagatcg
     9301 aattcgtccg cggcgaggga gtgcacctct gggacgcgga aggccgcgag tacctcgact
     9361 tcctcggcgg catcgcggtg gtcgccgccg gccacgccaa ccccaaggtg gtcgccgcgg
     9421 tcgccggcca gctcgccacc ctcggccaca cctcgaacct ctacttcacc ggcccgcagg
     9481 tccgcctcgc cgagcggctg gtggcggcgt tcggcggcga cggcaaggtg ttcttctcca
     9541 actccggcgc cgaggccatc gaggccgcca tcaaggtggc gcgccgctgg ggccgcgcca
     9601 accgtggcga gcgggccatc ggcatcgtct gcaccgacgg cgccttccat ggccgcaccc
     9661 tggccaccct ggccgccacc ggcaagcccg ccatcaaggc cgcgttcgaa ccactgcccg
     9721 ccggcttctc ccacgtcccc ttcggcgacg ccgacgccct cgccgccgcg atcggcccgg
     9781 acaccgccgc tgtgctcctg gagccggtcc agggcgagaa cggcatccgc gtgccgcccg
     9841 ccggctacct gcgggcggcc cgcgagctca ccgccgacgc gggcgtgctg ctgatcctcg
     9901 acgagatcca gtcgggcatg ggccgcaccg gccgcttctt cgcccaccag caccacggcg
     9961 tgcgtcccga cgtcgtcacc atggcgaagg cgctcggcag cggctacccg atcggcgcca
    10021 ccatcgccac caatgaggtg gccgacacca tgcaccctgg cgaccacggc accaccttcg
    10081 gcggcaaccc ggtcgcctgc gccgccgccc tcgccaccct cgacgtgatc gagccgctgc
    10141 tggacggcca cgtggacacg gtcgccgggc gccttcgcga cggtc
//
LOCUS       DASWDC010000235         7246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_311755, whole genome shotgun sequence.
ACCESSION   DASWDC010000235 DASWDC010000000
VERSION     DASWDC010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311755"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..553
                     /gene="coaD"
                     /locus_tag="VF995_07645"
     CDS             65..553
                     /gene="coaD"
                     /locus_tag="VF995_07645"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX9343471.1"
                     /translation="MTIACCPGTYDPVTNGHLDIVARAAGVFDRVIVAVLLNPAKQPM
                     FTVEERIAMLKEATADLPGVEVDTFSGLLVDFARARSVTAIVKGLRAVSDFDYELQMA
                     QMNLRLSGIETMFMTTNPKWSYLSSSLVKDVARHGGDVGGLVPEVVLARLRERVGRGA
                     NG"
     gene            682..1347
                     /locus_tag="VF995_07650"
     CDS             682..1347
                     /locus_tag="VF995_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEX9343472.1"
                     /translation="MNELAISQTLTVNVRELLHQPGAHKHVVQRGPVPDLATPAARVD
                     PASLVTVDAEIESVVEGLLVTGQVSARATLECARCLRQIEQDLRVDVRELFVLDPRAE
                     RQSSAPGARHERPRSPQGSPQAGEARHRPATGALTEDEGYAVLPDDRLPLETMARDAI
                     VLAFPAAPLCRADCAGLCPECGADRNATDCGHRPSADARWSALAGLELGAPGQDEQDG
                     DPD"
     gene            1423..1599
                     /gene="rpmF"
                     /locus_tag="VF995_07655"
     CDS             1423..1599
                     /gene="rpmF"
                     /locus_tag="VF995_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX9343473.1"
                     /translation="MPVPKRKTPRTKTRQRRAHWKSTAPTYAVCPQCRQAKLPHTVCQ
                     NCGSYGGRQAIEVD"
     gene            1650..2537
                     /gene="rnc"
                     /locus_tag="VF995_07660"
     CDS             1650..2537
                     /gene="rnc"
                     /locus_tag="VF995_07660"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003957601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX9343474.1"
                     /translation="MSPSYVRPFKRLPIERALGIRFGNDDIYVRALTHRSYAYENGGL
                     PTNERLEFLGDAVLGVVITDLIYRAFPLLPEGQLAKLRAATVNMNVLADVARELGVGE
                     AVRLGRGEELSGGRDKSSILADTLEALLGAVYLSKGLPRTGALIRRLFEARVMEAAGR
                     GAALDYKTSLQELAASSLGGMPSYAIEEEGPDHAKRFTATVSVAGVAYGSGQGRSKKE
                     AEQHAAREAFEALATSSMVTGSDPDDDAGSDGEGAGASGAAAGAGAADATVTPARGKS
                     GKVAKVAAGKPKPAGRQRD"
     gene            2564..3424
                     /locus_tag="VF995_07665"
     CDS             2564..3424
                     /locus_tag="VF995_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX9343475.1"
                     /translation="MPELPELDVLTENLTAQLAGARVRALRLVSVAALKTFDPPLDTL
                     AGAVVSGAGRRAKYVWLELVADGQGMFLVIHLSLGGRLSLGPKPASRRVAVLTIEFDD
                     GRVLSMTEGGTQRRAAVWLVRDPAQVAGLAGLGPEPLDPDFTPERLAKILAAGGHTLK
                     RMLTDQRELSGVGNAWSDDVLHRARLSPFARPERLDPEQVARLHAALVGVLTEARGAL
                     AEQVGGAVEIPKASERLFAVHGRAGAACFVCGDTIRSVWMGERETSYCPTCQTGGRVL
                     ADRRRSRFLR"
     gene            3524..4399
                     /locus_tag="VF995_07670"
     CDS             3524..4399
                     /locus_tag="VF995_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX9343476.1"
                     /translation="MAELPEVETLRHDLDKEIVGRKIRDVEVAASRVVRRHRNRPDFC
                     RQLSARRVAAIGRSGPHLLIGLDSGDTLVLTLGASGRLLRERSAAPVERHTNAVITFA
                     TGGGLRVLGLAADGELFVLGAGDRDGLAALGHAALDPLADAVAWPSFGAMVTAGRGPL
                     RALLTGGALLAGVGPIYADEILWASGLRFDHDAGSLRAQEVRRLHRAVQEILQEAVRL
                     RGSSVGDDPWLDLHGNKGEYQERLRAYQREGQPCQRCRSPLVAERLAHGDAQSDADSD
                     AQIGDVTYYCPRCQS"
     gene            4429..5187
                     /locus_tag="VF995_07675"
     CDS             4429..5187
                     /locus_tag="VF995_07675"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9343477.1"
                     /translation="MAGLVVLLVLPAALAGCGRSGSAPPAPAAPAAPVVTRPAAPVDG
                     PVTLGAAPAYVALARTDVTVRSKPGGGALVGLFPQQLGWNTTTSFLVRQARRVGGKVW
                     YQVQLPRRPNQASGWVRADQTQLAPLVWRVDVSLTHHQASLVHNGRLVRAFTVAVGQP
                     GTPTPTGAFYLMFKLHPPQISRVYGAFALGLAAWSNVLNQFGTGDGQIALHGTANTWE
                     LGHDVSHGCVRFDNEAITYLAGVLPIGTPVTIHA"
     gene            5192..5482
                     /locus_tag="VF995_07680"
     CDS             5192..5482
                     /locus_tag="VF995_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX9343478.1"
                     /translation="MTQPFGGMQRLEAVVRGRVQGVGFRYSVLEIARHAGLRGFAENK
                     PDGSVFVLAEGLPDQLDDLEKFLRRGPSFAHVAAVEADRSTATGEFDGFVAR"
     gene            5811..5996
                     /locus_tag="VF995_07685"
     CDS             5811..5996
                     /locus_tag="VF995_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343479.1"
                     /translation="MSKALMGFVGQPPAYMILRELNALKARVAELERALAAAEQEIAL
                     REVGDFRSSTEKEAALA"
     gene            6223..>7246
                     /locus_tag="VF995_07690"
     CDS             6223..>7246
                     /locus_tag="VF995_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9343480.1"
                     /translation="MFLKSLTLRGFKSFADKTVLHFEPGITVIVGPNGSGKSNVVDAI
                     AWVLGEQGAKSLRSGRMEDVIFAGTATRPPLGRAEVSLTIDNSAGLLPIDYGEVTIAR
                     HLYRSGESEYSVNGDACRLLDLQELLSDTGIGRELHTILGQNRVEEVLQGRPEDRRAA
                     IEEAAGIYKHRRRKDKALRKLAGMEQHLVRLSDLVGELRRQLKPLQQQAETARKAAEV
                     QAELREVRLTLRSRELATARERAAVLDAEEARFAARLAELEAVHQAATADEARLQAVL
                     TAEEPRLALAGEIAHRLDTVRERLRGTMALAEERARHLAQPLAEEDQGRSAADLEREA
                     AALTDEL"
BASE COUNT         1038 a         2629 c         2523 g         1056 t
ORIGIN      
        1 cgcttgggct ggagtcgcct tcggacctgt cggtggagtc gcccccggaa ggaggccgcc
       61 agctgtgacg atcgcctgct gccccggtac ctatgatccg gtgacaaatg ggcatctcga
      121 tatcgtcgcg cgcgcggcag gggtgttcga tcgggtgatt gtcgcggtgc tgctgaatcc
      181 ggcgaaacag ccgatgttca cggttgagga gcgcatcgcg atgctgaaag aggccaccgc
      241 cgaccttccg ggcgtcgaag tcgacacctt ttcgggcctg ctggtcgact tcgcccgggc
      301 gcggtcggtg accgcgatcg tcaaggggtt gcgggcggtc agcgatttcg actacgagct
      361 gcagatggcg cagatgaacc tccgtctctc cgggatcgag acgatgttca tgaccaccaa
      421 cccgaagtgg tcgtatctca gctccagcct ggtcaaggac gtcgccaggc atggcggcga
      481 cgtcggcggc ctggtccccg aggtcgtgct ggcccgcctg cgggagcggg tcgggcgcgg
      541 cgcgaacggc tgagccgacg gcgggggttc gcgctgtttt ctgctaccct tccccgggcg
      601 gcttcggccc cgagcgaccc gtgcccgcgt gtccccgccg cgagtgccgc ctcatcacac
      661 cagcgccaca agagttttgc catgaatgag ctcgccatat cgcaaacgct gacggtcaac
      721 gtccgtgaac tgctgcacca gcctggagca cacaagcacg tggtccagcg tggccccgtg
      781 cccgacctgg ccaccccggc cgcccgggtc gacccggcct cgctggtgac ggtcgacgcc
      841 gagatcgaat cggtcgtcga ggggctgctg gtcaccgggc aggtcagcgc ccgggcgacg
      901 ctggagtgcg cccgctgcct gcgccagatc gagcaggacc tgcgggtcga cgtccgcgaa
      961 ctgttcgtcc tcgacccgcg cgcggagcgc cagtcaagtg ctccaggcgc gcgccatgag
     1021 cgtccgcggt ccccgcaggg gtccccgcag gcgggagagg cgcggcaccg acccgcgacc
     1081 ggcgccctga ctgaggacga gggctacgcc gtgctccccg acgaccgtct gcccctggag
     1141 acgatggcca gggacgccat cgtgctcgcc ttcccggccg cgccgctgtg ccgtgcggac
     1201 tgcgccgggc tctgcccgga gtgcggcgcc gaccgcaacg cgaccgactg cggccaccgg
     1261 cccagcgccg acgcgcgctg gtccgcgctg gccgggctgg agctcggcgc gccgggccag
     1321 gacgaacagg acggcgaccc ggactgacac gaggcgagca cgcgagcagg aaccaaccca
     1381 ggaccaggac cgggaagacc cggccaggca gaaggagacc agatgcccgt tccaaagcgg
     1441 aagacgccgc gcaccaagac gcggcagcgg cgtgcgcact ggaagagcac cgcgccgacc
     1501 tacgccgtct gcccacagtg ccgccaggcg aagcttccgc acaccgtctg ccagaactgc
     1561 ggctcctatg gcgggcgcca ggccatcgag gttgactgaa acaccgagcc acctcccgac
     1621 aatcccctgg gaagcagggt gatccagcga tgtcaccgtc atatgtcagg cccttcaagc
     1681 ggctcccgat cgagcgggcg ctcgggatcc gcttcggcaa cgacgatatc tacgtcaggg
     1741 cgctgaccca ccgttcctac gcctacgaga atggcgggct gcccaccaac gagcggcttg
     1801 agttcctcgg cgacgccgtc ctcggcgtgg tcatcaccga cctgatctac cgcgccttcc
     1861 ccctgctccc ggaaggccag ctcgccaagc tgcgggcggc caccgtgaac atgaacgtcc
     1921 tggccgacgt ggcccgggag ctgggagtgg gcgaggcggt caggcttggc cgcggcgagg
     1981 agctctccgg tgggcgcgac aagtcctcca tcctcgccga caccctggag gcgctgctcg
     2041 gcgcggtcta cctgagcaag ggcctgcccc gcaccggcgc cctgatccgc cgcctgttcg
     2101 aggcgcgggt catggaggcc gccgggcgcg gcgccgcgct cgactacaag acctcgctgc
     2161 aggagctggc tgcctccagc ctcggcggca tgccgagcta cgccatcgag gaggagggcc
     2221 ccgaccacgc caagcgcttc accgccaccg tctcggttgc cggcgtcgcc tacggcagcg
     2281 gccagggccg ctccaagaag gaggcggagc agcacgcggc ccgcgaggcg ttcgaggcgc
     2341 tggcgacctc gagcatggtc accggctccg accccgacga tgacgccggc agcgacggtg
     2401 agggcgcggg cgcctcgggc gcggcggcgg gcgcaggcgc cgccgacgcc acggtgaccc
     2461 cggcacgggg caagagcggc aaggtcgcca aggttgccgc cggcaaaccg aagccggccg
     2521 gccggcagcg cgactaggcg ccgggacagg gggacaggct ggcgtgcccg agcttccaga
     2581 gctcgacgtc ctgaccgaga acctcacggc gcagctcgct ggcgcgcggg tgcgcgcgct
     2641 gcggctcgtg tcggtggctg ccctcaagac cttcgaccca cccctcgaca cgctggcggg
     2701 tgcggtcgtc agcggtgccg gccgccgcgc caagtacgtc tggctggagc tcgtggccga
     2761 cgggcagggg atgtttctgg tcatccacct gtcgctcgga gggcggctgt cgctcgggcc
     2821 gaagccggcc agccgcaggg tggcggtgct gaccatcgag ttcgacgacg gccgggtgct
     2881 gtcaatgacc gagggcggca cccaacgccg cgccgcggtc tggctggtcc gggatcctgc
     2941 gcaggtggcg gggctggccg ggcttggacc ggagccgctc gacccggact tcaccccgga
     3001 gcggctcgcc aagatcctcg ccgccggtgg ccacaccctc aagcgcatgc tgaccgacca
     3061 gcgggagctg tcgggggtcg gcaacgcctg gagcgacgac gtgctgcacc gcgcccgcct
     3121 ctcgcccttc gcgcggcccg agcgcctcga ccccgagcag gtcgcccgcc tgcacgccgc
     3181 cctggtcggc gtgctcaccg aggcgcgcgg cgccctcgcc gagcaggtcg gcggggcggt
     3241 cgagatcccc aaggcgtccg agcgcctgtt cgccgtccac ggccgtgccg gcgccgcctg
     3301 cttcgtctgc ggcgacacga tccgctcggt ctggatgggg gagcgggaga cctcctactg
     3361 cccgacctgc cagaccggcg ggcgcgtcct cgccgaccgg cggcgctctc gcttcctgcg
     3421 ctgagcagcg gccgccgggc gcccgcgcct ccccgccggc ccgcgggtgc ttgctaaact
     3481 cggcgctccg cggcgtctgg cgtcttcgga gcggaggggg cgggtggccg aactacccga
     3541 agttgagaca cttcgtcatg atctggacaa agagatcgtt ggccggaaga tcagggatgt
     3601 cgaggtggcg gccagccggg tcgtccgccg ccaccgcaac cgtcccgact tctgccgcca
     3661 gctgagcgcc cgcagggtcg ccgcgatcgg gcgcagcggc ccgcacctgc tgatcggcct
     3721 ggattccggc gacactctgg tgctgacgct cggcgccagc ggccggctgc tgcgcgaacg
     3781 cagcgccgcc ccggtggagc ggcacaccaa cgccgtgatc accttcgcca ccggcggcgg
     3841 cctacgggtc cttggcctgg ccgccgacgg ggagctgttc gtgctcggcg ccggcgaccg
     3901 tgatgggctg gccgcgctcg gccacgccgc gctcgacccg ctggccgacg cggtcgcctg
     3961 gccgtcattc ggcgccatgg tcaccgctgg acgcgggccg ttgcgggcgc tgctcaccgg
     4021 cggcgcactg ctggccgggg tcgggcccat ctacgctgac gagatcctct gggccagcgg
     4081 gctgcgcttc gaccacgacg cgggcagcct gcgcgcccag gaggtccggc gcctgcaccg
     4141 tgcggtccag gagatcctgc aggaggcggt gcggctgcgg ggcagctcgg tcggcgacga
     4201 tccctggctc gacctgcacg gcaacaaggg cgagtaccag gagcgactcc gcgcctacca
     4261 acgcgaaggg cagccctgcc agcgctgccg ctccccgctg gtcgccgagc ggctcgcgca
     4321 cggcgacgcg cagagcgacg cagacagcga cgcacagatc ggcgatgtca cttactattg
     4381 cccgagatgc cagagctgag cagcttgttt cctcgacgtt cggtcggttt ggccggcctt
     4441 gtcgtgctgc tggtcctgcc ggcggcgctc gccggctgcg gtcgctcggg ttcggcgccg
     4501 cccgcgcccg ccgccccggc ggcgccggtc gtcaccaggc cggccgcgcc cgtggacggg
     4561 ccggtcacct tgggcgccgc ccccgcctac gtggcgctgg cacgcaccga cgtcaccgtc
     4621 cgctccaagc ccggcggcgg cgcgctggtg gggctgttcc cgcagcagct cggctggaac
     4681 accaccacct cctttctggt ccgccaggcc cggcgcgtcg gcggcaaggt ctggtatcag
     4741 gtgcagctcc cgcgccgccc caaccaggcc agtggttggg tccgcgccga ccagacccag
     4801 ctcgcgccgc tggtctggcg ggtggacgtc tcactcacgc accatcaggc cagcctggtc
     4861 cacaacgggc ggctggtccg cgccttcacg gtggcagtcg gccagccggg tacgcccacc
     4921 ccgacggggg cgttctacct gatgttcaag ctgcacccgc cgcagatctc acgggtctac
     4981 ggcgccttcg cgctcggcct ggccgcctgg tcgaacgtgc tcaaccagtt cggcaccggg
     5041 gacggccaga tcgcgctgca cggcaccgcc aacacctggg agctcggcca tgacgtctcc
     5101 cacggctgcg ttcggttcga taacgaggcg attacctacc ttgctggcgt gctgccgatc
     5161 ggcacgccgg tcacgatcca cgcctagacc gatgacccag cccttcggcg gcatgcaacg
     5221 actagaggcg gttgtgcggg ggcgcgtcca aggcgttggt ttccgctata gcgtccttga
     5281 aatcgcccgc catgctggtt tacgtggttt tgcggagaac aagcctgacg gctctgtatt
     5341 cgtgctcgcc gaagggcttc ccgatcagct cgatgacctg gagaagttcc ttcggagagg
     5401 cccaagcttc gcccatgtcg ccgccgtcga ggccgaccgt tccacggcga ctggtgagtt
     5461 tgatggcttc gtcgcccgct aaggcatttg tgcagctcat cgagggtgtc aagcgtgctt
     5521 cgtccgtaac acgctacttg gcgtatcagc tcggacgtgt tatcgtcccc ttgtgaaatc
     5581 aagtctctgg aaccgccctg atgcggcacc cggcacggtc gtgctccctc ccaatggcgg
     5641 gaacggtcct cgggtactcg tcaggccttt ctcatccaga gaaccataag gagtttgcta
     5701 gccgtcggag tattccgttg cggccgaaat ggtgtgtcgt cacgctggcc gaacggacgg
     5761 atcgcagcgg tcgccgattg agatcgttgc tgaggggagg atcttcccgg atgtcgaagg
     5821 cgctcatggg cttcgtaggt cagccgcccg catacatgat cctgcgggag ctgaatgccc
     5881 tcaaggcgcg ggtggctgag ttggaacgtg ctcttgcggc cgcggagcag gagattgccc
     5941 ttcgcgaggt cggggacttc cggtcctcga ccgagaagga ggcggccctg gcctgacgcc
     6001 aggctttttc tttgcacggg accctcgctg agggtcccgt ttcttgttcc cgggtgactc
     6061 ccgggtggct cccgggtggc gtccggccgc catgctagac tgcgcttctg ccgggtaaag
     6121 gctcaagcgg gcgccgtctc acctggacgg cagaccagga aggaccccgg atgccccggt
     6181 gagtgaggac cgggcagtct cagaaaggtc tccggctatc gcgtgttcct gaagtcgctc
     6241 actctccgtg ggttcaagtc gttcgcggac aagacggtcc tgcacttcga gcccgggatc
     6301 accgtgatcg tcgggccgaa cgggtcgggc aagtccaacg tcgtcgacgc gatcgcctgg
     6361 gtgctcggcg agcagggcgc gaagtcgctg cgctcgggcc gcatggagga cgtgatcttc
     6421 gccggcaccg ccacccgccc gccgctcggc cgcgccgagg tcagcctcac gatcgacaac
     6481 tccgccggcc tgctgccgat cgactacggc gaggtcacca tcgcccgcca tctgtaccgc
     6541 tcgggcgaga gcgagtactc ggtgaacggc gacgcctgcc gcctgctcga cctgcaggag
     6601 ctgctctccg acaccggcat cggccgcgag ctgcacacca tcctcggcca gaaccgcgtc
     6661 gaggaggtcc tgcagggccg ccccgaggac cgccgcgccg ccatcgagga ggccgccggc
     6721 atctacaagc accgccgccg caaggacaag gcgctgcgca agctcgccgg catggaacag
     6781 cacctggtgc gcctctccga cctggtcggc gagctgcgcc gccagctcaa gcccctgcag
     6841 cagcaggccg agaccgcccg caaggccgcc gaggtccagg ccgagctgcg cgaggtacgc
     6901 ctcaccctcc gcagccgcga gctggccacc gcccgcgagc gcgccgccgt cctcgacgcc
     6961 gaggaggccc gcttcgccgc ccgcctcgcc gagctggagg ccgtccacca ggccgccacc
     7021 gccgacgagg cccgcctcca ggccgtcctg accgccgagg agccccgtct cgcgctggcg
     7081 ggggagatcg cccaccgcct cgacaccgtg cgcgaacgcc tgcgcggcac catggccctg
     7141 gctgaggagc gcgcccgcca cctcgcccag ccgctcgccg aggaggacca gggccgctcc
     7201 gccgccgacc tggagcgcga ggccgccgcc ctcaccgatg agctcg
//
LOCUS       DASWDC010000236         5658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_616868, whole genome shotgun sequence.
ACCESSION   DASWDC010000236 DASWDC010000000
VERSION     DASWDC010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616868"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VF995_07695"
     CDS             complement(<1..1302)
                     /locus_tag="VF995_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343481.1"
                     /translation="MLEHHVRARVAGLPNVALRHGHVEDVLAGRGARSAAVTVTGVRL
                     AGDERLHADLVVDASGRAARSLRWLERLGLGCVAPPLSAVHVDMGYATRILRRDPGDG
                     RDWRVAIVIGDPPSSAGVAFALEDEQWMVTLAGFHGDHPPTTASGWAEFAATLPDPAI
                     AELIAESEPLSAIVPHRMAASLRRHVERLHQRQLAGGLVLLGDAVASFNPIYGQGISA
                     AALQAEALATALDRCDGLGAGFVRTYNRLAGRAVAAPWRLSVGGDFALPQTTGAKPWG
                     TALTTSYLRRTLQAAQHDEVVAQRLVEVTHLLRPPSALLAPAVALRVRTAWGVICDCQ
                     RSPAPVSDDQRCSFRLSYLPATLANMRSRAAPFVAAPPTGARIRTRLRVEEPDAQVLW
                     LVGTHLGRLASLDLAVRCQLGADDDQRTERQRALTAASSSRW"
     gene            1777..2490
                     /locus_tag="VF995_07700"
     CDS             1777..2490
                     /locus_tag="VF995_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9343482.1"
                     /translation="MGRQKKHGEHTREALLQAAEALLADGGEQALSVRAVAERVGTST
                     RAVYSVFGSKDGLLSALGQHGFELLSAVVDELPPTDDPIADIVEGGVMGFRRWWRRNP
                     RLFRLAFDHLVVAGPGGVKVSQAGLAALGRLKLRVQRASDAGLLGGCDVDEVTAQIEA
                     LCEGLTIVEGRHGPLLHSEPEEIWREALRALLDGLRGATSAPLPREGGAGGDLSAVAL
                     LPGEPGPVGDRSSGPDPPS"
     gene            complement(2717..2890)
                     /locus_tag="VF995_07705"
                     /pseudo
     CDS             complement(2717..2890)
                     /locus_tag="VF995_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473529.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2894..3073)
                     /locus_tag="VF995_07710"
                     /pseudo
     CDS             complement(2894..3073)
                     /locus_tag="VF995_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3571..4251)
                     /locus_tag="VF995_07715"
     CDS             complement(3571..4251)
                     /locus_tag="VF995_07715"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEX9343483.1"
                     /translation="MTTKASAVSYAERGLLVAAAPAGAPAGLPKAVRKALLGCGCGPL
                     GCAVAGWAPAPCADPLRASRLWRGGPEAGAGVGLLTGMDAGLEVLDVPQALGAAVLAL
                     GRPGLGPVAETACGRFHFYVAPSGAGPCITVPGRGAGGPSGIRWLGDGSFVLAPPSRG
                     ASGGAVRWHRTLDAPLPSALRVLDRLRDVEAAGPLTPGKSDNGASIPAPVIPIGFPVG
                     IALPGGAR"
     gene            complement(4274..4786)
                     /locus_tag="VF995_07720"
     CDS             complement(4274..4786)
                     /locus_tag="VF995_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343484.1"
                     /translation="MRWFRYLLAGGVVAAALLPTAANAAGSEHIGLHDMTCNGIGAMG
                     TGLPANARLNLKLVDKDNGKTLATQMLTTSASGTFSTTVKARLNQVLGMRLVVSDGQN
                     MTVAFADHQMEPGMAMCTLPFTGGADHTLPLVVFGAVLLSVGAGLLVFGSHTHAYARF
                     RARHGRHAAA"
     gene            complement(4815..>5658)
                     /locus_tag="VF995_07725"
     CDS             complement(4815..>5658)
                     /locus_tag="VF995_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343485.1"
                     /translation="GSVRRGCDRAGMQTTTAPKHLRLGRRDEFDLVPRPPRDENVAME
                     LFLAVVALVLSVALIVGALTRHSGSGTAAISPAASPVPAAAPAAAPSQGAAASPAPTT
                     MPAAKGGVGSGAMTQMAMGGPNLIANPGFETGLDGWRPIGGATLRRTATAKEGGFSAS
                     FATGATSNPGISFPSATRTAAKKNYSGTVWVYPAAPGTLIELRLVEYQNGKRLAVDTV
                     GEQLDSAQWQPVQVVHLAHHPGDTLGIEVLAPGLPVGSPVLVDDASIQQKSGSYMSVT
                     TTTR"
BASE COUNT          749 a         2010 c         2165 g          734 t
ORIGIN      
        1 ccagcggctc gacgacgccg cggtgagcgc ccgctgacgc tcagtgcgct gatcgtcatc
       61 ggcgccgagc tggcagcgga ccgccagatc caggctggcg aggcggccaa ggtgggtgcc
      121 gaccagccac agcacctgcg catcgggttc ttccacgcga aggcgggtgc ggatgcgcgc
      181 accggtgggt ggtgcggcga cgaacggtgc ggcacgggaa cgcatgttcg ctagcgtagc
      241 aggcaagtat gacaatcgga atgagcatcg ctggtcgtca ctgacaggtg ctggtgaacg
      301 ctgacagtca cagatgacac cccaagcagt ccgaacccgc agggcgaccg ccggggcgag
      361 cagggcggag ggcgggcgca gcaggtgggt gacctcgacg aggcgctggg ccacgacttc
      421 gtcgtgctgg gccgcctgca gggtccgccg caggtaggag gtggtcaggg cggtgcccca
      481 gggtttggcg ccggtggtct gggggagggc gaagtcaccg ccgaccgaca ggcgccaggg
      541 cgccgccacc gcgcgaccgg ccagccggtt gtaggtgcgg acgaagccgg cgccgaggcc
      601 gtcgcagcgg tcgagggcgg tcgcgagcgc ctcggcctgc agcgccgccg cgctgatccc
      661 ctgcccgtag atcgggttga agctggccac ggcgtcgccg agcagcacca gcccgccggc
      721 cagctggcgc tggtggaggc gctcgacgtg gcggcgcagg ctcgcggcca tccggtgggg
      781 gacgatcgcg ctgagcggct cggactcggc gatcagctcg gcgatcgccg ggtcgggcag
      841 ggtggcggcg aactcggccc agccggaggc ggtggtgggc gggtggtcgc cgtggaagcc
      901 ggccagggtc accatccact gctcgtcctc cagggcgaag gcgacgccgg cggagctggg
      961 cgggtcgccg atgacgatgg cgacacgcca gtcgcggccg tcgcccgggt cgcggcgcag
     1021 gatccgggtg gcgtagccca tgtcgacgtg gacggccgac agcggcggcg ccacgcagcc
     1081 gaggcccagc cgctccagcc agcggagcga gcgggccgcg cggcccgagg cgtcgaccac
     1141 caggtcggcg tggaggcgct cgtccccggc gaggcggacc ccagtgacag tgacagcggc
     1201 gctgcgggcc ccccggccgg cgagtacatc ttcgacatgg ccgtgccgca gcgccacgtt
     1261 cggcagcccg gcgacccggg cgcggacgtg atgttcgagc attgggcgcg agcagagcgc
     1321 gcccggatgg gaatggtcgg cgtcccgcag acgcaggcgc accccccagc ctggaaccag
     1381 cggagttgcg cgccgcggaa cggcagggcg ccgtgggcga gcaattcccc cccgagtcct
     1441 gggaaccagg cctggagccg ctgctgccca gagagcagca gggcgtgcgc gtggcgcgcc
     1501 tgcggaacgc ccctgcggaa ggccggctcc cccggaagct cgtcgtggtc gatcacgagc
     1561 acctcgccga agcgctggga cagcacgcgc gcggcgcaaa gccccgccat gctcgctccg
     1621 acgacgatcg ctctcgaacc ctgacggcgc tcggccattc ccgcatccct cgccaacatt
     1681 gcgataaccg cggtattcgt aataacgatc atcccgatat cgcaacaatg ttacgttatg
     1741 gcgtaacgta acaacgttgc gttacagtgt caacccatgg gacggcaaaa aaagcacggg
     1801 gagcacaccc gcgaggccct gctccaagcc gccgaagcgc tgctcgccga cggtggcgag
     1861 caggcgctgt cggtgcgtgc cgtggccgag cgggtcggca cctccacccg tgccgtctac
     1921 agcgtgttcg gctccaagga cggcctgctg tcggccctcg gccagcacgg cttcgagctg
     1981 ctcagcgcgg tggtcgacga gctccccccg accgacgacc cgatcgccga catcgtggag
     2041 ggcggggtca tgggcttccg ccgctggtgg cgccggaacc cgcggctgtt ccgcctggcc
     2101 ttcgaccatc tggtggtggc cggccccggg ggggtcaagg tgagccaggc cggcctggcc
     2161 gcgctcggcc ggctgaagct gcgggtgcag cgcgccagcg acgccggcct gctcggcggc
     2221 tgcgacgtgg acgaggtgac cgcccagatc gaggcgctct gcgagggcct gaccatcgtg
     2281 gagggccgcc acggcccgtt gctgcactcc gaaccggagg agatctggcg tgaggcgctg
     2341 cgggcgctgc tcgacggcct gcgcggcgcg acctcggcgc cccttcctcg cgagggcggg
     2401 gccggcggcg atctcagcgc cgtcgcgctc ctgcccggcg agccggggcc ggtgggggat
     2461 aggagctccg gtcccgaccc gccgagctag gagcctacgg gcgcgcaacc ctggctcgct
     2521 cgatctgggc ctggcgtgcg gcggcgaggg cctggcggcc cgccttggtg atcgaggcct
     2581 gggcctcgtc gatgacgacc aggccgcggc cggcgagggc gcgggcgacc gactggctga
     2641 cgggccaggt gtcggcgtga ccctgcccgg cttccagcag ttcgagggcg gagcgctccg
     2701 ccgggctgag gcgccacagc agcgcccgga cggccgcctc ggggaagcgg tagtggccgc
     2761 cgatggtgcg cacggccggc aggcggccct gcttggccca gcggcccacc gggcgctggt
     2821 ccaccccgaa cagctcggcg acctcgccgg gccgcagcag gcggtcctgc gtctccgccc
     2881 cggccccgat cgcggtcggg acgccggcct ggagctgctc gttgatggcg ctctcgggga
     2941 agcggtagtg gccgccgagg gtgcggatcg ccggcaggcg ccccttgcgg gcccactgct
     3001 tgacggtccg ggggccgacg ccgaagcgct cggcgacctc gccgacccgc agccagcggt
     3061 ccttgggcgc cagcgccgtc cgccggctgg cggccccggc cccggccccg gtcccggttc
     3121 cggtcccgtt gccggtgccg ttgcggccat ggccgttccc gttgccgttc ccgttgccgt
     3181 tgtggccgtt gccgttgtgg ccgttggccg ggcgggcgcc cggtccgacg accttggccg
     3241 ccggggcctg gcggacgact gccccgggcg ccgcgggcgc cctggcccca gagacgcccg
     3301 gcctcggccc gacgacattg gcgcccgggg caggtcgagc gaccgtgacg gccgcgctgg
     3361 cggtcggtcc gaccaccttg ggaagcgcgg ccgttgccgg tcccacaacc ttgccgttga
     3421 ctcgataggg ggcgccgcta ccactgcgcg cccggttcgg cccgacgacc ttggcgctgg
     3481 gcgccgggcc gacgaccttg agggcggggg cggggccgac caccttgctg gtggcggcgc
     3541 tcgccgggtt ggggtggatg atcttgcggg tcatcttgct cctcctggaa gcgcgatccc
     3601 gacggggaac ccgatgggga tgaccggtgc tgggatggaa gcgccgttgt ccgacttccc
     3661 gggagtcagg ggtccggcgg cctcgacgtc acgcaggcgg tccagcaccc ggagggcgga
     3721 cggcagcgga gcgtcgaggg tgcggtgcca gcggacagcc ccgccgctgg caccgcggga
     3781 tgggggagcg agcacgaagc tgccatcccc gagccagcgg atcccggagg gtccaccggc
     3841 cccgcgccct ggcacggtga tgcacgggcc tgcaccagac ggcgcgacat agaagtggaa
     3901 ccggccacag gcggtctcag cgaccggccc cagaccaggc cttcccaggg cgagcacggc
     3961 ggcgccgagc gcctggggca cgtcgagcac ctccaggccg gcgtccatgc cggtcagcag
     4021 cccgacgcca gccccggcct cggggccgcc ccgccacagg cgcgacgccc gcagcgggtc
     4081 ggcgcagggc gccggcgccc agccggccac cgcgcagccg agcggtccgc agccgcaccc
     4141 cagcagcgcc ttgcgcaccg ccttcggcag gcccgctggc gctccagcgg gggcagctgc
     4201 caccagcagc ccgcgttcgg cgtaggagac tgcgctcgcc ttggtggtca tggtcaaaac
     4261 ctcaagttca ggttcaggcc gcggcgtggc ggccgtggcg ggcgcggaag cgggcgtagg
     4321 cgtgggtgtg ggaaccgaag accagcaggc cggcgccgac gctcagcagc accgcgccga
     4381 acaccaccag cggcagggtg tggtccgcgc cgccggtgaa cggcagggtg cacatcgcca
     4441 tgcccggctc catctggtgg tcggcgaacg ccaccgtcat gttctggccg tccgacacca
     4501 ccaggcgcat gccgaggacc tggttgagcc tggccttcac cgtggtcgag aaggtccccg
     4561 acgccgaggt ggtaagcatc tgggtggcga gggtcttgcc gttgtccttg tcgaccagct
     4621 tcaggttcag ccgggcgttg gccggcaggc cggtgcccat cgcgccgatg ccgttgcagg
     4681 tcatgtcgtg caggccgatg tgctcgctgc cggcggcgtt cgccgccgtg ggcaggagcg
     4741 ccgcggccac cacgcccccc gccagtaggt acctgaacca ccgcatccgg atcacgagtc
     4801 cctttctctt gtggttagcg ggtggtggtc gtcaccgaca tgtacgaccc gctcttctgc
     4861 tggatggagg cgtcgtcgac cagcaccggg gagccgaccg gcaggcccgg cgccagcacc
     4921 tcgatgccga gcgtgtcgcc ggggtggtgg gccaggtgga ccacctggac cggctgccac
     4981 tgggcggagt cgagctgctc gccgacggtg tcgaccgcca ggcgtttgcc gttctggtac
     5041 tcgaccaggc gcagctcgat cagcgtcccc ggcgccgccg ggtacaccca gacggtgccg
     5101 gagtagttct tcttcgccgc ggtcctggtc gccgagggga agctgatgcc agggttggag
     5161 gtcgcgccgg tggcgaagct ggccgagaat ccgccctcct tggcggtcgc ggtgcggcgc
     5221 agggtcgcgc cgccgattgg ccgccagccg tccaggcccg tctcgaaacc cgggttggcg
     5281 atcaggttcg gcccgcccat ggccatctgg gtcatggccc ccgaccccac gccgcccttg
     5341 gcggcgggca tggtggtcgg cgccggcgag gccgcggcgc cctggctggg cgccgcggcc
     5401 ggggcggcgg ccgggacggg actggctgcc ggggagatcg ccgccgtgcc ggaccccgag
     5461 tgccgcgtga gcgccccgac gatcagcgcg acactcagca ccagcgcgac gacggcgagg
     5521 aacagttcca tcgccacgtt ctcgtcccgc ggcggccggg gaaccagatc gaactcgtcc
     5581 cgccggccga gccggaggtg cttgggagca gttgtcgttt gcatgcccgc acggtcgcag
     5641 ccccgcctaa cggacccc
//
LOCUS       DASWDC010000237         5504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1480384, whole genome shotgun sequence.
ACCESSION   DASWDC010000237 DASWDC010000000
VERSION     DASWDC010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480384"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VF995_07730"
     CDS             <1..1076
                     /locus_tag="VF995_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEX9343486.1"
                     /translation="KNNTDGKGTSYGTHENFLVDRTTPFTRLVRDLTPFLCSRQIFAG
                     AGSLVRDPADNSVAYQIAQRSQFFEVEVGLETTLKRPIINTRDEPHADPERYRRLHVI
                     IGDANMNEVATFLKTGSLAIILNLIEDDAVPGEEWQLANPVQALRAIAKDPTLRTTVE
                     RKDGRMVTACEIQWAYLDAAKKYLKDREIAHWQVEVLERWEAVLTQLEEDPFTAHRTV
                     DWVSKYQLMRQIAERDGLELTDPKLALVDLQYHDVRLDRGLYYRLAAAGRVERLVAEQ
                     EIARAVSEPPTDTRAYFRGRCLARYASQIAAAGWDAIIFDTGRETLQRVPMLEPTRGT
                     AVHTAAILEASPAAEDLIRNLQG"
     gene            1167..1388
                     /locus_tag="VF995_07735"
     CDS             1167..1388
                     /locus_tag="VF995_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEX9343487.1"
                     /translation="MASGSGSGGGAEQKRRATGKQDAEQEDVQASADVTEKGEALKDE
                     LDSLLDEIDDVLEENAEEFVKQYIQKGGQ"
     gene            1466..2266
                     /gene="prcB"
                     /locus_tag="VF995_07740"
     CDS             1466..2266
                     /gene="prcB"
                     /locus_tag="VF995_07740"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEX9343488.1"
                     /translation="MSDAVRGLARYSPSFVDALREHAPELLQQPGVTLGGQQVPEVPH
                     GTTVAAICYADGVVVGGDRRSTEGYAIAQRDVEKVFPADDFSAVAIAGAAGPAIEMVR
                     LFQTELEHYEKVEGLPLSLEGKANKLGQMVRGNLGMAMQGLVVVPLFCGYDQRRLTGR
                     IFRYDVTGGRWEDADYHANGSGGKDARATIKKRYRHGMDRESAADILVEGLYDAAEED
                     AATGGPDLIRGIFPVVATVTAEGYRRLPDEDVRKAAERVVAARARGEG"
     gene            2270..3010
                     /gene="prcA"
                     /locus_tag="VF995_07745"
     CDS             2270..3010
                     /gene="prcA"
                     /locus_tag="VF995_07745"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEX9343489.1"
                     /translation="MSIGFGYVPPEQIIKDKADYARKGIARGRPMVVLEYDGGILFVT
                     DNPSTHLHKVAELYDRIAFAAVGRFSEFENLRVAGITLADIRGYQYGREDVTSRSIAN
                     AYSQELGRIFMEGPKPYEVELCVAEVGSGSTPNALYHIGFEGTVTDHKRHLAMGGQAD
                     DLNAELQRNYTDGIGLDQALRLAVSALSKATQSGDGGPAAGRLEVAVLERDRPRRAFR
                     RLTSDDLNARLRSDDSAGDSHEAGEGPV"
     gene            3091..4473
                     /locus_tag="VF995_07750"
     CDS             3091..4473
                     /locus_tag="VF995_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343490.1"
                     /translation="MEPPSGHGRRRLVLVLVAVALVAASGGGVLIAKTSGPHGQGHAA
                     APATTAPATTAPATASTTAPPATATVSGALAAQIHQVEAAVERLRQLRFVRGVPVVVE
                     SPQKMAAQLLKEINADTNDARLQDLDRALETLGELPPHTDLPKLLRAVQTESVLGFYV
                     PGKAPAKGRLYVRSTNGLDPYARFVLSHELTHAVTDQHYDLTRGDRLDAPGKDDQSTA
                     YTALLEGDATVMMGRYHDQVLTPAEQADADRVGAAQTTPRLDAAPASLRESLLFPYEA
                     GVAFVRALYARGGWAAVDRAYLDPPTSSEQILHPEKYFGTRDQPKPVVVPDLSGSLGS
                     GWRSATVAGWGEFDVRLMLEGTLGVATAERAAAGWGGGELRSFQDGAKTAVALRTVWD
                     SSAEASEYCGLLANWATSHFGAPTAASGRRWAGSGQQTTLMCRGTAATWLSAPDGPSL
                     DRLTAGIGTT"
     gene            4470..>5504
                     /locus_tag="VF995_07755"
     CDS             4470..>5504
                     /locus_tag="VF995_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15"
                     /protein_id="HEX9343491.1"
                     /translation="MNRAARRLVAFAVPVAVVAALLGSTRASAPPPLFSSGLLGNATA
                     LAAIPPEAGEAQGAAYAPGSTVLRLADGRLRYLPAGASDPVTVPAGDPGANARIAADR
                     AWLALGTVPGANAAERDAASRSLLFLRLLQRPGGGVIAAATPYWADVWPRDASFAAAA
                     FSVTGHRTEAAAILAFLARTQRPDGTWPARSHPDGTPVDDGRAPELDADGWVPWAAWL
                     ASGQGHDHVLAAWSWPMVRAAAEAAASALRAGGLPAASPDYWERNERQPTLGTAAALL
                     TGLRAAADLAAALGHGQEGARWAAAAVRLAAATRRSFAGPAGYHRYPAPSSASSRAFG
                     DAAGDAAGDAG"
BASE COUNT          844 a         2092 c         1926 g          642 t
ORIGIN      
        1 acaagaacaa caccgacggc aagggcacct cctacggcac ccacgagaac ttcctggtcg
       61 accggaccac ccccttcacc cggctggtcc gcgacctgac cccattcctg tgcagccgcc
      121 agatcttcgc cggggccggc agcctggtgc gcgaccccgc cgacaactcc gtcgcctacc
      181 agatcgccca gcgctcccag ttcttcgagg tcgaggtcgg cctggagacc accctcaaac
      241 gaccgatcat caacacccgc gacgagccgc acgccgatcc cgagcgctac cgccgcctgc
      301 acgtgatcat cggcgacgcc aacatgaacg aggtcgccac cttcctgaag accgggtcgc
      361 tggcgatcat cctcaacctg atcgaggacg acgccgtccc cggcgaggaa tggcagctcg
      421 ccaacccggt ccaggcgctg cgcgcgatcg ccaaggaccc gaccctgcgg accaccgtgg
      481 agcgcaagga cggccgcatg gtgaccgcct gcgagatcca gtgggcctac ctggacgccg
      541 ccaagaagta cctcaaggac cgcgagatcg cccactggca ggtcgaggtc ctggaacgct
      601 gggaagcggt cctcacccag ctggaggagg accccttcac ggcccaccgc accgtcgact
      661 gggtcagcaa gtaccagctg atgcgccaga tcgccgagcg ggacggcctg gagctgaccg
      721 acccgaagct cgccctggtc gacctgcagt accacgacgt gcggctggac cgcggcctgt
      781 actaccgcct cgccgccgcc gggcgggtcg agcggctggt cgccgagcag gagatcgccc
      841 gcgcggtgtc cgagccgcca accgacacgc gcgcctactt ccgcggccgc tgcctggcca
      901 ggtacgcctc gcagatcgcc gcggcgggct gggacgcgat catcttcgac accggccgcg
      961 agaccctcca gcgggtcccg atgctggaac ccaccagggg cactgccgtg cacaccgccg
     1021 ctatcctcga ggccagcccg gcagccgaag acctgatccg caacttgcag ggatagcctg
     1081 tacccaagtt cgccagtccg ggtcgtccgg actcggtggc ccaaccgtgg cagaatcgcc
     1141 agatcagtac gtcaaggagg gacgccatgg ccagcggcag cggcagcggc ggcggcgccg
     1201 agcagaagcg gcgcgcaacc ggcaagcagg acgccgagca agaagacgtc caggcatcgg
     1261 ccgacgtcac cgagaagggg gaagccctta aggacgagct ggacagtctt ctcgatgaga
     1321 tcgatgacgt actagaggaa aatgcagaag aatttgtgaa acagtacatc cagaagggcg
     1381 ggcaatagcc gatcgacatc ggcggcaagc ggaacgagaa gaagaagcga atatgccaag
     1441 cggaatttga cgtgaaggga gcggtgtgag cgacgcggtc cgcgggctgg cgcggtacag
     1501 cccatcgttt gtcgatgcgc tgcgggagca tgcgccagag ctgctgcagc agcccggcgt
     1561 gacgctgggc gggcagcagg tgcccgaggt gccgcatggg accacggtgg cggcgatctg
     1621 ttacgccgac ggggtggtcg tcggcgggga ccggcgctcg accgaggggt acgcgatcgc
     1681 gcagcgggac gtcgagaagg tcttcccggc ggatgacttc tcggcggtgg cgatcgccgg
     1741 ggcggccggg ccggcgatcg agatggtgcg gctgttccag accgagctgg agcactacga
     1801 gaaggtcgag gggttgccgc tgtcgctgga gggcaaggcc aacaagctcg gccagatggt
     1861 gcgcggcaac ctcggcatgg ccatgcaggg cctggtggtg gtgccgttgt tctgcggcta
     1921 cgaccagcgc cgcctgactg gccgcatctt ccgctacgac gtcaccggcg gccgctggga
     1981 ggacgccgac taccacgcca acggctccgg cggcaaagac gcccgcgcca ccatcaagaa
     2041 gcgctaccgc cacggcatgg accgcgagag cgccgccgac atcctggtcg agggcctcta
     2101 cgacgccgcc gaggaggacg ccgccaccgg cgggcccgac ctgatccggg ggatcttccc
     2161 ggtggtggcg accgtgaccg ccgagggcta ccggcgcctg cccgacgagg acgtgcgcaa
     2221 ggcggccgag cgcgtggtcg ccgcccgcgc ccgaggggag ggctgagcgg tgtcgatcgg
     2281 cttcggctat gtgccccccg agcagatcat caaggacaag gccgactacg cccgcaaggg
     2341 catcgcgcgc ggccggccga tggtggtgct cgaatacgac ggcggcatcc tgttcgtcac
     2401 cgacaacccc tcgacccacc tccacaaggt cgccgagctc tacgaccgga tcgccttcgc
     2461 ggcggtggga cgcttctcgg agttcgagaa cctgcgggtc gccgggatca ccctggccga
     2521 catccgcggc taccagtatg gccgcgagga cgtcacctcc cggtcgatcg ccaacgccta
     2581 ctcccaggag ctgggccgca tcttcatgga ggggcccaag ccctacgagg tcgagctgtg
     2641 cgtcgccgag gtgggcagcg gcagcacccc caacgcgctg taccacatcg gcttcgaggg
     2701 caccgtcacc gaccacaagc gccacctcgc catgggcggc caggccgacg acctcaacgc
     2761 cgagctgcag cgcaactaca ccgacggcat cggccttgac caggccctgc gactggcggt
     2821 cagcgcgctc tccaaggcga cccagagcgg cgacggcggt ccggccgccg gccggctgga
     2881 ggtcgccgtg ctggagcgtg accggccccg gcgcgccttc cggcgcctga ccagcgacga
     2941 cctgaacgcc cggctccgct cagacgacag cgcgggcgac tcacacgaag caggcgaagg
     3001 cccggtatga ccgactgggg cgagccagcc cctccgcctg acccgtccgc ttcgccaacg
     3061 ccgccccagc cccagcccca gcgcggcccg gtggagccgc cgtcaggcca cggcaggcgc
     3121 cggctcgtcc tcgtcctcgt cgcggtggcg ctggtggccg cctccggcgg cggcgtgctg
     3181 atcgccaaaa ccagcggccc ccatggccag ggccacgccg ccgcgccagc caccacggcg
     3241 cccgcgacga ccgcccccgc cacggcctca accacggcac cgccggccac cgcgaccgtg
     3301 tcgggggcgc tggccgccca gatccaccag gtggaggcgg ccgtggagcg gctgcgccag
     3361 ctccgctttg tccgcggcgt gccggtggtc gtcgagtcgc cccagaagat ggcggcgcag
     3421 ctgctcaagg agatcaacgc cgacaccaac gacgcccgtc tccaggacct cgaccgggcc
     3481 ctggagacgc tcggggagct gccgccccac accgacctgc cgaagctgct gcgcgccgtc
     3541 cagaccgaga gcgtgctcgg cttctacgtc cccggcaagg cgcccgccaa gggccgcctc
     3601 tacgtccgca gcaccaacgg cctggacccc tacgcccgct tcgtgctctc ccacgagctg
     3661 acccacgcgg tcaccgacca gcactacgac ctgacccgcg gcgaccgcct cgacgcgccc
     3721 ggcaaggacg accagtcgac cgcgtacacg gcgctgctgg aaggcgacgc gacggtgatg
     3781 atgggccggt atcacgacca ggtgctcacc cccgccgagc aggccgacgc cgaccgggtc
     3841 ggcgccgcgc agaccacccc gcggctggac gcggccccag cgagcctgcg cgagtcgctg
     3901 ctgttcccct acgaggccgg ggtcgcgttc gtgcgggccc tgtacgcgcg gggcggctgg
     3961 gcggcggtcg accgcgccta cctcgaccca cccacctcca gcgagcagat cctccacccc
     4021 gagaagtact tcggcacccg tgaccagcca aaaccagtcg tggtgccgga cctttccggg
     4081 tcgcttggca gcggctggcg cagcgccacc gtggctggct ggggcgagtt cgacgtccgc
     4141 ctcatgctgg agggcaccct cggggtcgcc accgccgagc gggccgccgc cggctggggc
     4201 ggcggtgagc tgcgcagctt ccaggacggc gccaagaccg ccgtggcgct gcggaccgtc
     4261 tgggactcat ccgccgaggc cagcgagtac tgcgggctgc tggccaactg ggcgaccagc
     4321 cacttcggcg ccccgaccgc cgccagcggg cgccgctggg ccgggtccgg ccagcagacc
     4381 accctcatgt gccgaggcac cgccgccacc tggctctccg ccccggacgg gcccagcctc
     4441 gaccggctca ccgcgggcat cgggacgact tgaaccgggc ggcccggcgg ctggtcgcct
     4501 tcgcggtgcc ggtggcagtc gtggcggcgc tgctcgggtc gacccgggcg agcgcgccgc
     4561 cgccgctgtt cagcagcggg ctgctcggca acgccaccgc gctggcggcg atcccgccgg
     4621 aggcggggga ggcccagggc gcggcctacg cgccgggcag caccgtgctg cggctcgccg
     4681 acgggcggct gcgctacctg ccagccgggg ccagcgaccc ggtgaccgtc cccgccggcg
     4741 acccgggcgc caacgcgcgg atcgccgctg accgcgcctg gctcgcgctc gggacggtgc
     4801 ccggcgccaa cgccgccgag cgggacgccg ccagccgctc gctgctgttc ctgcggctgc
     4861 tgcagcgccc cggcgggggg gtgatcgccg cggccacgcc ctactgggcc gacgtctggc
     4921 cgcgcgacgc gagcttcgcg gccgctgcct tcagcgtcac cggccaccgg acggaggctg
     4981 ccgcgatcct cgccttcctg gcccgcaccc agcggcccga cgggacctgg ccggcgcgct
     5041 cccacccgga cggcaccccg gtggacgacg gccgggcgcc ggagctggac gccgacgggt
     5101 gggtgccctg ggccgcctgg ctggccagcg ggcagggcca cgaccacgtc ctggcggcgt
     5161 ggtcgtggcc gatggtccgc gccgccgccg aggcggctgc cagcgcgctg cgcgccggcg
     5221 gcctcccggc tgccagcccg gactactggg agcgcaacga gcgccagccg accctcggca
     5281 ccgccgcggc gctgctcacc gggctccgcg ccgccgccga cctggccgcc gcgctcggcc
     5341 acggccagga gggggcccgc tgggcggcgg cggcggtgcg gctcgccgcc gcgacccggc
     5401 ggtcgttcgc cggccccgcc ggctaccacc gctacccggc accgtcctcg gcctccagcc
     5461 gcgccttcgg cgacgccgca ggcgacgccg caggcgacgc gggc
//
LOCUS       DASWDC010000238        12159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1118610, whole genome shotgun sequence.
ACCESSION   DASWDC010000238 DASWDC010000000
VERSION     DASWDC010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12159
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118610"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VF995_07760"
     CDS             <1..427
                     /locus_tag="VF995_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX9343492.1"
                     /translation="FGREPNLPLLRDELRRALAGQLPLEQRLAEREAAYRTPTPPLPR
                     PSAEAEVRFPDRASDRATVIEVRCPDGPAVLYRITTALATCGVTITLAKIATYGAEVV
                     DTFYLHDPSGRPTLNPETRTAIVAAVLGALAPVTSRGVL"
     gene            complement(405..896)
                     /locus_tag="VF995_07765"
     CDS             complement(405..896)
                     /locus_tag="VF995_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343493.1"
                     /translation="MIDSRDAAREVGRESQSANAGGIRQVPLLAAYLIAGVILGLVTL
                     ALHPMSRVLSNQLASHLLLPYAAAGALFFTGRAVARDKLRWCRSCCRWLAVQPDGERV
                     VRSRPITRRNHGRLEQFNTYGQRTGYSRSQTTTRGIRETVAITMVCHHCGAKSIVRER
                     REK"
     gene            1366..1563
                     /locus_tag="VF995_07770"
     CDS             1366..1563
                     /locus_tag="VF995_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343494.1"
                     /translation="MRVAFSFLFLLTIIIVVMLRFRHKGDGLTVFEFLLCGAWGFLLA
                     SSSFAPTIRNVLNDLVRAISR"
     gene            1628..1903
                     /locus_tag="VF995_07775"
     CDS             1628..1903
                     /locus_tag="VF995_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343495.1"
                     /translation="MRPTRPDDQRQPQYHQLTPEAKSRLGRAAAKNQPRRRRRFRPLH
                     TLLIAPLQRLFVLTVRTMWQVARPWLVPLLLLAGGARLLLWYVQHHH"
     gene            complement(1924..2496)
                     /locus_tag="VF995_07780"
     CDS             complement(1924..2496)
                     /locus_tag="VF995_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9343496.1"
                     /translation="MARVLVAEDEVLIRVDVVETLEEASHTVVGEAGDGEQAVKLARE
                     LAPDLVVMDVKMPKMDGLEAARQITAAGGAVLVLTAFSDKELVDEAADAGTIGYLVKP
                     FQPAQLVAAVSVALARVAETRDLQQTVEELEAKLEARKVIERAKGRLMEQFDLTEDQA
                     YTRMRRAAMDRQLPLAEIARRVLEASQPKS"
     gene            complement(2520..7151)
                     /gene="gltB"
                     /locus_tag="VF995_07785"
     CDS             complement(2520..7151)
                     /gene="gltB"
                     /locus_tag="VF995_07785"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX9343497.1"
                     /translation="MAGGVPQESPGRLYDARRDERDACGIGFVADAEGRASRVIVEYA
                     LGGLERLRHRGAVAADARTGDGAGLLLPLPRQFFAGGCGEDGDRSDSDRSDSGDQALV
                     GVAMAFLPRDGAAAAHARAAVEQALAAEQLKLVRWRAVPVDPSALGDAARASQPRIEQ
                     AVFIASDPSGGPAAAMGRARGGSGRGTVPPEPPGHDGEEAERRCFRAGRRAALQAKAA
                     GVPLYLASCSTRTVTYKALCAADQLAAFYTDLADARMVAPFVVFHQRYSTNTLPSWER
                     AQPFRFLCHNGEINTLDGNVAWMRAREGSLGSGDLAPEELLRPVLDPDGSDSAMLDNA
                     VELLVRGGRDIRHALAMLVPAAVEHDPRASRALRDFYRFHACLTEPWDGPAGLVFTDG
                     RLVGAALDRNGLRPLRFEACDDGLVVCASEVGAVDLAGHGGVRRGRLGPGQMLCVDPE
                     AGLLEDAAIKAALAGRRPWSVWVAEHLRELDQGNPVTIPAPGSEAAAALVPAQVAFGF
                     TSELVSAVLRPMATGGKEPTASMGDDTALAPFGHTLRPVSHFLRQRFAQVTNPPIDHL
                     RERHVLSKRTLLGPRQPLLSEAPEAAKLIELPGFLLTPEGLERLQDPEHDLNPVTLDA
                     TWPVAEGRDGLRAACLRLADRAEAAVRGGAGLLVISDREAEVGGERAPVPSLLAVGAT
                     QQRLVWAGLATRASVVVDSGEPVESHQAAALLGYGAEAICPRVTLVTVAGLANDGDPA
                     TGQPAAARAQRRYVAALEEAVFKVLSKMGISTLDSYRGAQIFEALGLDSDVIATCFTG
                     TASPLGGIGFAELADDVLARVSLAQEAGARLGNPGWFKHRPKGEYHQTNPPVMQALHH
                     TLNEEGSLRGSKRGAHLLQQAVKGGGYERYRHFAKLVNERPPAALRDLLEARPAGPAV
                     PLDEVEPAAVILRRFSTAAMSLGSLSPEAHETLAIALNRVGGRSNSGEGGEDPARFGT
                     ERSSAIKQVASGRFGVTPEYLANAAELQIKIAQGSKPGEGGQLPGHKVSAEIARLRHT
                     QPGVALISPPPHHDIYSIEDLAQLVFDLKQANPEADVTVKLVAEAGVGTVAAGVVKSL
                     ADVVTVSGADGGTGASPLSSIKHAGGPWELGLAETQQALQANGLRSRVRLRIDGGLKS
                     GRDVLLAALLGADEYGFGTAALLAEGCLMVRTCHQDNCPVGIATQRPDLRAKFAGTPD
                     MVVHYLEYVAEELRELLAWLGLRSLDEAVGRVDLLAQRTTGHARADALDLRPLLQRAP
                     EARGAARRFEAPDPTQRARSALGDRLAEDAMPGLLAGDLVNLRYQIGNTDRSVGARLG
                     VAVGAAFGSSPPHGRARVEFDGVAGQSFGAFLAPGIDFRLTGAANDYVGKGMGGGRIV
                     VHGPAGDRGDAVLVGNTVLYGATRGELFCAGRAGERFAVRNSGATAVVEGTGDHCGEY
                     MTGGTVVVLGPVGRNVGAGMTGGELFLYDGGGDEALEHLNDELVLARRPDDVQLVFLR
                     ELVVAHAELTGSAVASRILDRWDQAVGLFWRVAPRAEVAVIERAHEGTG"
     gene            7373..8881
                     /locus_tag="VF995_07790"
     CDS             7373..8881
                     /locus_tag="VF995_07790"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9343498.1"
                     /translation="MQKEIPRPARKRLDSKATEHLHRLMAAWQLIADLAFADLLLFTA
                     VEGTENFRIVGQLRPYTARTLYPSDLVGEEVEPGWHPFVERAWLEQRRLRSPQPIFID
                     AEPVRVEAVPVRHKGTVIAALSVEAAEAPVRPTGRLEAAYLRAADALLEMVAGGTFPF
                     KEAHDLIASPRVGDGLVVLDSSGRVEFASPNATSAFRRMGTNSLPLGQPLPSPAGIMV
                     AEAMAGRRPIESDVDMDGAVTDLRVVPLLRAHQRRGALVLCREVTELRRRDRVISRRE
                     ATIREVHHRVKNNLQTVASLLRLQARRLDDHPMAVAALEESVRRIAAIALVHETLTEE
                     LEGAVDMAEVARRVVRMLEGSLGREDVRIVVDAQSVRVNAHVATPLSVVLNELIQNAV
                     EHGLGNGPGTITVHLHATPTDHLELEVVDEAATRPGPPPRAGGAPEVPQPAAPGGLLT
                     SDPDGTTGGLGLRLVRGLVEEELGGRFSLIHRIPPAHGSIARATIPPQETPA"
     gene            9083..9598
                     /locus_tag="VF995_07795"
     CDS             9083..9598
                     /locus_tag="VF995_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343499.1"
                     /translation="MTALRSAVVVGALLLAAAGCGGSGKKPAASPTSQAPTQSTAASG
                     APGASAIASQPMALPKGTARVDLLAVDRSAANTVTIRWRVTNISSQQVNLEGAMERES
                     AKFEPNRPADGVSLVDATGNKRYFPLYSTAGQCLCPAHDATKVDLEPGKSAEFSAVMP
                     APRRRCSRSPS"
     gene            9595..10608
                     /locus_tag="VF995_07800"
     CDS             9595..10608
                     /locus_tag="VF995_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9343500.1"
                     /translation="MIPLTPLFQAVPIGSSPRPFSGTEIDPAKTSLKVPEIVPLISTA
                     EGTVESTDATGADRSVRLSADVLFAINKSNLTPAAQSVLKQVAQQVDASSGTTVRVDG
                     YTDNSGNDAINNPLSVARAQAVETQLKALVSRQGVSFQAAGHGSADPVADNGSEEGRR
                     KNRRVTVTFARPAPQTSSGSGSAAPAPAAPAPAWTGGKLPVVATAASVGDNVKSVKLE
                     INSVHRDAAGLTTMTWTLTNTGTAKFGVGFTFVDRGQEFNYDGLNVPGARLVDQAGKL
                     TYNPLRDEGKECVCTNLNKGKGDLDPGESVTYFDSYELPPGVASVDITFPFFAPAQHV
                     QVQ"
     gene            10634..10810
                     /locus_tag="VF995_07805"
     CDS             10634..10810
                     /locus_tag="VF995_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343501.1"
                     /translation="MVKDGASSEAKPSSQKEIRAGRPDTRTEGSETFEARILALQKQG
                     KTYEEALQSLEDGE"
     gene            complement(10821..11318)
                     /locus_tag="VF995_07810"
     CDS             complement(10821..11318)
                     /locus_tag="VF995_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9343502.1"
                     /translation="MAVEPPPRPVPSGDPRAWSQRVGGSLRNVVVAADGSQASLHAVA
                     LAGQIASGAGAQLTVLHVRPEAPVFGGLTPASCGMIEQWREDLEYEVGIRVTETLDPL
                     GIRWRFRVENGDPVHRIDQLAAAVDADLIVVGSSGSGLLRILLGGSTATRLVRRERRP
                     VLVVR"
     gene            complement(11432..11707)
                     /locus_tag="VF995_07815"
     CDS             complement(11432..11707)
                     /locus_tag="VF995_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343503.1"
                     /translation="MADYHIFETPDTLDQYKQASAKFLDALGRVVYPVLREDPTNESG
                     RFPISYEDGWYTFEVETAGGGLYYLAYQAIEELHRVLVFRLTTFREP"
     gene            complement(11707..11871)
                     /locus_tag="VF995_07820"
     CDS             complement(11707..11871)
                     /locus_tag="VF995_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343504.1"
                     /translation="MDHEHVRKVISQQLAELDPLARRLRTALLRGESPESLGLVENGL
                     ATVLRERGIE"
BASE COUNT         1804 a         4453 c         4230 g         1672 t
ORIGIN      
        1 cttcggccgc gagcccaact tgccgctgct ccgcgacgag ctgcgccgcg ccctcgccgg
       61 ccaactcccc ctggaacagc gcctcgccga acgcgaggcc gcctaccgca cccccacccc
      121 acccctacca cgccccagcg ccgaggccga ggtccgcttc cccgaccgcg cctccgaccg
      181 cgccaccgtc atcgaggtcc gctgccccga cgggcccgcc gtcctctacc gcatcaccac
      241 cgccctggcc acctgcggtg tcaccatcac cctcgccaaa atcgccacct acggcgccga
      301 ggtggtcgac accttctacc tccacgaccc ctcgggccgc ccgaccctca atcccgagac
      361 gcgcaccgcg atcgtggccg ccgtgctcgg cgcgctcgcc ccggtcactt ctcgcggcgt
      421 tctctgacga ttgatttggc gccgcagtgg tggcagacca tggtgatggc gacggtctcg
      481 cggatgcccc gggtggtggt ctgggagcgg ctgtagccgg tgcgctggcc gtaggtgttg
      541 aactgctcca ggcggccgtg gttgcggcgg gtgatcgggc ggctgcggac gacgcgctcg
      601 ccgtccggct ggaccgcgag ccagcggcag catgagcggc accagcggag cttgtcgcgg
      661 gccaccgccc tgccggtgaa gaacagcgcc ccggcggcgg cgtagggcag cagcaggtgg
      721 ctggcgagct ggttggaaag gaccctggac atcgggtgga gcgcgagggt caccagcccc
      781 aggatgacgc ccgcgatcag gtaggcggcg agcagcggca cctggcggat gccccccgcg
      841 ttggcgctct ggctctcccg gccgacttcc cgtgcggcat ctctgctgtc gatcatctgc
      901 agcggccccc cgcggcgatc ccgtgattgc tgtgacgcat cgctaccagg tcaatcagcc
      961 tggtcgacgg tgataaccat cctcaaatcc cgcgcgaagt ccaggcgatc ttcgtaggga
     1021 acctcatcag accgctggca gcaaattggc agctccacac ttagacgttg aacaacccat
     1081 tgcctcgtaa agatggagat gccgtccaga tcgaatgaaa agcctgagca tggcgaaccg
     1141 gagtaacaag catgttatac ttcagcctgg aggcatccgg gcgaacccgt ccagccagcg
     1201 cagaccggcg acgggaatcg cgcggatgac tctttcattg actaggtccc cctttacact
     1261 tagactcaat ggcgtggttt cgacttttcg cccgaaagat ctcgcagtca tgccgccatt
     1321 tgacgggatc ggcgtcaccc cggttcatga aaggacgatg cgccgatgag agtcgccttc
     1381 tcgttcctct tcctgctcac catcatcatc gtggtgatgc tccgcttccg gcacaaaggt
     1441 gacgggctca ccgtgttcga gttcctgctc tgcggcgcgt ggggtttcct gcttgcctcg
     1501 tcgtcgttcg caccgacgat ccgcaacgtc ctcaacgacc tggtgcgagc gatcagccga
     1561 tagcggggcc acgactccca gcagcaccca cccggtccgg gaaccacggc agcaagcgag
     1621 gcagatcatg cgcccaaccc ggcccgacga tcagcgccag ccccagtacc atcagctcac
     1681 tccggaggcg aagagccgcc tgggtcgggc ggcggccaag aaccagcccc ggcgcaggcg
     1741 gcgcttccgc ccgctgcaca cgctgctgat cgcaccgctg caacgcctgt tcgtgctcac
     1801 cgtgcgcacc atgtggcagg tggcgcgtcc ctggctggtg ccgctgctgc tgctggccgg
     1861 cggcgcgagg ctgctcctct ggtacgtgca acaccaccac tgagaccgtg cgcgtggcgg
     1921 ccgctacgac ttcggctggg acgcctccag cacgcggcgg gcgatctcgg ccagcggcag
     1981 ctgccggtcc atggccgcgc ggcgcatgcg ggtgtaggcc tggtcctcgg tgaggtcgaa
     2041 ctgctccatg aggcggccct tggcccgctc gatcaccttg cgagcctcca gcttggcctc
     2101 cagctcctcg acggtctgct gcaggtcgcg cgtctcggcg acgcgggcga gggcgacgct
     2161 gacggcggcg accagctgtg ccggctggaa cggcttcacc aggtagccga tggtgccggc
     2221 gtcggcggcc tcgtcgacca gctccttgtc cgagaaggcg gtcaacacca gcaccgcgcc
     2281 gcccgcggcg gtgatctggc gggccgcttc cagcccgtcc atcttcggca tcttcacgtc
     2341 catgaccacc aggtcgggag ccagctcgcg ggcgagcttg accgcctgct cgccgtcgcc
     2401 ggcctccccc acgacggtgt ggctggcctc ctccaaggtc tcgacgacgt ccactcggat
     2461 caggacctcg tcttcagcca cgagcacccg cgccatcccg ctcctctcca aacactgacc
     2521 tatccggtgc cctcgtgggc ccgttcgatg acggcgacct ctgccctcgg ggcgacccgc
     2581 cagaacaagc ctaccgcctg gtcccagcgg tcgaggatgc ggctggccac cgcggatccg
     2641 gtcagttcgg catgggcgac gaccagctcc cgcaggaaga ccagctggac gtcatctggc
     2701 cgccgggcca gcaccagctc gtcattcagg tgctcaaggg cctcgtcgcc gcctccatcg
     2761 tagaggaaca gctccccgcc ggtcatgccg gcgccgacat tgcgcccaac ggggcccaaa
     2821 accaccacgg tcccgccggt catgtactca ccgcagtggt cgccggtccc ctcgaccacg
     2881 gcggtggcgc ccgaattgcg caccgcgaac cgctccccgg cccgcccggc gcagaacagc
     2941 tccccccggg tggcgccgta caggacggtg ttgccgacca gcaccgcgtc gccacggtcg
     3001 cccgccgggc catgcaccac gatccggccg ccgcccatgc ccttgccgac gtagtcgttg
     3061 gcggcgccgg tgagccggaa gtcgatcccg ggggccagga aggcgccgaa gctctgcccg
     3121 gcgacaccgt cgaactcgac ccgggcgcgg ccgtgcggcg ggctcgaccc gaacgcggcg
     3181 ccgaccgcca cgccgaggcg ggcgccgacc gagcggtcgg tgttgccgat ctggtagcgc
     3241 aggttgacca ggtccccggc gagcaggccg ggcatggcgt cctcagcgag gcggtcgcca
     3301 agcgccgacc gggcccgctg ggtcgggtcg ggcgcctcga agcgccgcgc ggccccccgg
     3361 gcctcgggcg cccgctggag gagtgggcgc aggtcgagcg cgtccgcccg cgcgtgcccg
     3421 gtggtccgct gcgccagcag gtccacccgg ccgaccgcct cgtccaggct ccgcaggccg
     3481 agccaggcaa ggagctcccg cagctcctcg gcgacgtatt ccaggtagtg gaccaccatg
     3541 tcgggggtgc cggcgaactt ggcgcgcagg tccggccgct gggtggcgat cccgaccggg
     3601 cagttgtcct ggtggcaggt ccgcaccatc aggcagccct cggccagcag cgccgccgtg
     3661 ccgaagccgt attcgtcggc gccgagcagc gcggccagca gcacgtcccg gccgcttttc
     3721 aggccgccgt cgatccgcag ccgcacccgc gagcgcagcc cgttggcctg gagggcctgc
     3781 tgcgtctcgg ccaggcccag ctcccagggc ccgccggcgt gcttgatcga cgacagcggg
     3841 ctggcgccgg tgccgccgtc ggcgccggag accgtcacca cgtcggccag ggatttcacc
     3901 accccggcgg cgaccgtgcc gacgccggcc tcggcgacca gcttgacggt cacgtcggcc
     3961 tccgggttgg cctgcttcag gtcgaagacc agctgggcga ggtcctcgat cgagtagatg
     4021 tcgtggtgcg gcggcggcga gatcagcgcc acgcccggct gggtgtggcg caggcgggcg
     4081 atctcggcgc tgaccttgtg gccggggagc tggccgccct cgccgggctt ggagccctgg
     4141 gcgatcttga tctgcagctc ggcggcgttg gcgaggtact cgggggtgac cccgaagcgg
     4201 ccggaggcga cctgcttgat ggcggagctg cgctcggtgc cgaagcgggc cgggtcctcg
     4261 ccgccctcgc cggagttgct gcggccgccg acgcggttga gggcgatcgc cagggtctcg
     4321 tgcgcctccg gcgacagcga ccccaggctc atcgcggcgg tcgagaagcg ccgcaggatg
     4381 acggcggcgg gctcgacctc gtccagcggc accgccgggc cggccgggcg ggcctccagc
     4441 aggtcgcgca gcgcggcggg cggccgctcg ttgaccagct tggcgaagtg gcggtagcgc
     4501 tcgtagccgc cccccttgac ggcctgctgc agcaggtgcg ccccgcgctt ggagccgcgc
     4561 aggctgccct cctcgttgag agtgtggtgc agcgcctgca tcaccggcgg gttggtctgg
     4621 tggtactcgc ccttgggcct gtgcttgaac cagccggggt tgccgagccg ggcgcccgcc
     4681 tcctgggcga ggctgacgcg ggcgagcacg tcgtcggcca gctcggcgaa gccgatgccg
     4741 cccagcggcg aggcggtccc ggtgaagcag gtggcgatca cgtcgctgtc gaggccgagc
     4801 gcctcgaaga tctgggcgcc ccggtaggag tccagggtgg agatgcccat cttcgagagg
     4861 actttgaaga cggcctcctc cagcgccgcg acgtagcggc gctgggcccg cgccgcggcc
     4921 ggttgtcccg tggccgggtc gccgtcgttg gcgagcccgg ccacggtgac caaggtgacc
     4981 cgggggcaga tcgcctcagc gccgtagccg aggagggctg ccgcctggtg cgactcgacc
     5041 ggctcaccgc tgtccaccac cacgctggcg cgggtggcga gccccgccca caccagccgc
     5101 tgctgcgtcg ccccgaccgc cagcagcgac gggaccgggg cgcgctcgcc ccccacctcc
     5161 gcctcccggt cggagatgac cagcaggccg gcgccgccgc gcacggccgc ctcggcccgg
     5221 tcggcgaggc gcaggcaggc ggcccgcagg ccgtcgcgcc cttcggcgac gggccaggtg
     5281 gcgtccagcg tcaccgggtt gaggtcgtgc tcggggtcct ggaggcgctc caggccctcc
     5341 ggggtgagca ggaagcctgg cagctcgatc agcttcgccg cttcgggcgc ctcgctgagc
     5401 agtggctggc gcgggccgag gagggtccgc ttcgagagca cgtggcgttc gcgcaggtgg
     5461 tcgatgggcg ggttggtgac ctgcgcgaac cgctggcgca ggaagtggct gaccggccgg
     5521 agggtgtggc cgaacggggc cagggcggtg tcgtcaccca tcgaggcggt cggctccttg
     5581 ccgccggtgg ccatcggccg cagcaccgcc gagaccagct cgctggtgaa cccgaaggcg
     5641 acctgcgcgg gcaccagcgc cgccgcggcc tcggagccgg gcgccgggat cgtcaccggg
     5701 ttgccctggt ccagctcccg gaggtgctcg gcgacccaga cgctccaggg ccgccggccg
     5761 gcgagggccg ccttgatcgc ggcgtcctcc agcaggccgg cctcggggtc gacgcagagc
     5821 atctggcccg gtccgaggcg gccccggcgc accccgccgt gcccggcgag gtcgacggcg
     5881 ccgacctcgg aggcgcacac caccaggccg tcgtcgcagg cctcgaagcg cagcgggcgc
     5941 aggccgttgc ggtccagcgc cgcgccgacc aggcggccgt cggtgaacac cagcccggcg
     6001 gggccgtccc acggctcggt caggcaggcg tggaagcggt agaagtcccg cagggcacgg
     6061 ctggcgcgcg ggtcgtgctc gacggcggcc gggaccagca tcgcgagggc gtggcggatg
     6121 tcgcgcccgc cgcgcaccag cagctccacg gcgttgtcga gcatcgccga gtccgacccg
     6181 tccgggtcga gcactggccg cagcagctcc tcgggggcca ggtcgccgct gccgaggctg
     6241 ccctcgcggg cgcgcatcca ggcgacgttg ccgtcgaggg tgttgatctc gccgttgtgg
     6301 cataggaagc ggaacggctg cgcccgctcc cacgacggca gggtgttggt cgagtagcgc
     6361 tggtggaaca ccacgaacgg cgccaccatc cgcgcgtcgg ccaggtcggt gtagaacgcg
     6421 gcgagctggt cggcggcgca cagcgccttg taggtcaccg tccgggtcga acacgacgcc
     6481 aggtagaggg gcaccccggc ggccttggcc tggagggcgg cccgccggcc cgcgcggaaa
     6541 catcgccgct ccgcctcctc cccatcgtgg ccggggggtt cggggggcac ggtcccgcgg
     6601 ccggaaccac cacgagccct tcccatggcg gccgcggggc cccccgatgg gtcggacgcg
     6661 atgaagacgg cctgctcgat gcgtggctgg ctggccctgg cggcgtcgcc gagcgcggac
     6721 gggtccaccg gtaccgcgcg ccatcgcacc agcttcagct gctcggcggc cagggcctgc
     6781 tcgacggcgg cgcgggcgtg cgccgccgcc gcgccgtcac gcggcaggaa cgccatggcg
     6841 acgccgacga gggcctggtc gccgctgtcg ctccggtcgc tgtcgctccg gtcgccgtcc
     6901 tcgccacagc ctccagcgaa gaactgccgg ggcagcggca gcagcagccc ggcgccgtcg
     6961 ccggtgcggg cgtccgcggc gaccgcgccg cggtggcgca ggcgctcaag gccgccgagc
     7021 gcgtattcga cgattaccct gctggcccgc ccttcggcgt cggccacgaa cccaattccg
     7081 caggcgtcgc gctcgtcgcg acgcgcgtca tagagccgcc caggggactc ttgggggacg
     7141 ccaccagcca ccgtctctcg cctccgatcg cggtcctgcc ggggcggacg ccggaggtgc
     7201 cgctcggccg tgtcgctgga ccgaactgga gagacagcgg tgtctcgggc atgaaaacgg
     7261 cgcccgccgg ggcgagcgcc attgcccttc acgtcttctc agcgaaggtt acgggcgtgt
     7321 gacgaactcg tcaacacaag cgcacctgtc gcggagggat accctgggtg gcgtgcaaaa
     7381 ggagataccc cggccagcgc ggaagcggct cgacagcaag gcgaccgagc acctgcaccg
     7441 cctcatggcg gcttggcagc tgatcgccga cctcgccttc gccgacctgc tcctgttcac
     7501 agcggtcgaa ggaaccgaga atttccggat tgtcggacaa ctccgcccct ataccgcccg
     7561 caccctgtac ccatcggatc tggtcgggga agaggtcgaa cccggctggc acccgttcgt
     7621 ggagcgggcg tggctggagc agcggcgcct gcggtcgcca cagccgatct tcatcgacgc
     7681 cgagccggtc cgggtcgagg ccgtgcccgt gcgccacaag ggcaccgtga tcgcggcgct
     7741 cagcgtcgag gccgccgagg cccccgtgcg gccgaccggc cggctggagg ccgcctacct
     7801 gcgcgccgcc gacgcgctgc tggagatggt cgcgggcggg accttcccgt tcaaggaggc
     7861 ccacgacctg atcgcctccc cccgggtggg cgacgggctg gtggtgctgg attccagtgg
     7921 ccgggtcgag ttcgccagcc ccaacgccac ctcggcgttc cggcggatgg ggacgaactc
     7981 gctgccgctc gggcagccgc tgccgtcgcc ggccgggatc atggtcgccg aggcgatggc
     8041 gggccgccgc ccgatcgagt ctgacgtgga catggacggc gcggtcaccg acctccgggt
     8101 ggtgccgctg ctgcgcgccc accagcgccg cggcgcgctg gtgctgtgcc gcgaggtgac
     8161 cgagctgcgc cgccgcgacc gggtcatctc ccgccgcgag gcgaccatcc gcgaggtcca
     8221 ccaccgggtc aagaacaacc tccagacggt cgcaagcctg ttgcgcctgc aggcgcgccg
     8281 cctcgacgac cacccgatgg ccgtcgccgc cctggaggaa tcggtccgcc gaatcgccgc
     8341 gattgccctg gtccacgaga ccctcaccga ggagctggag ggcgccgtcg acatggccga
     8401 ggtcgcccgc cgggtcgtcc gcatgctgga gggctccctc ggccgcgagg acgtccgcat
     8461 cgtggtggac gcccagtcgg tccgcgtcaa cgcccacgtc gccacccccc tttcggtggt
     8521 cctcaacgag ctgatccaga acgccgtcga gcacggcctc ggcaacggcc cggggaccat
     8581 caccgtccac ctccacgcca cccccaccga ccacctcgaa ctcgaggtgg tcgacgaggc
     8641 cgccacccgc ccgggcccgc cgcccagagc cggcggggcg cccgaggtcc cgcagcccgc
     8701 cgcgcctggc gggctcctca ccagcgaccc cgacggcacc accggcggcc tcggcctccg
     8761 cctggtccgc ggcctggtcg aggaggagct cggaggccgc ttctccctga tccaccgcat
     8821 ccccccggcg cacggcagca tcgcccgcgc gaccatcccg ccgcaggaaa ctcccgcctg
     8881 atccggccgc cgcgtcgcgt tctgccaacc gggtgcgcgt agctgtgccg cgtagctgtg
     8941 ccgcgtagct gtgccacgta gctgtgccgc gtagctgtgc ggagaaatca ccatcgcgcg
     9001 cgcttgcggg cttccactac catggcaagc ccccagcgca gaggagtaga acggcgactt
     9061 tatggcgtat gtaaagaatt cgatgacggc actccggtcg gcggtggtgg tgggcgccct
     9121 gctgctcgcg gcggccggat gcggcggctc cgggaagaag ccggcggcct ccccgaccag
     9181 tcaggcgccc acgcagagca cggcggcgtc gggagcgccg ggagcgagcg ccatcgcctc
     9241 gcagccgatg gcgctcccca aggggaccgc acgcgtggac ctgctcgcgg tggaccgcag
     9301 cgcggccaac acggtgacca tccgctggcg ggtcaccaac atcagctccc agcaggtgaa
     9361 cctggagggg gcgatggagc gggagagcgc gaaattcgag cccaaccggc ccgcggacgg
     9421 cgtcagcctg gtggacgcga ccgggaacaa gcgctacttc cccctgtaca gcaccgccgg
     9481 acagtgcctg tgcccggccc acgacgccac caaggtggac ctggagccgg gcaagtcggc
     9541 ggagttctcc gcggtcatgc ccgccccccg gcgtcgctgc agcaggtcgc cgtcatgatc
     9601 ccgctgacgc cgctgttcca ggcggttccg atcggttcga gcccccggcc gttcagcggc
     9661 acggagatcg acccggcgaa gacctcactc aaggtgccgg agatcgtccc gctgatcagc
     9721 accgcggaag gcactgtcga gtcgaccgac gccaccggcg cggaccggag cgtcaggctc
     9781 tccgccgacg tcctgttcgc gatcaacaag tcgaacctga cgccggccgc gcagagcgtc
     9841 ctgaagcagg tcgcccagca ggtcgacgcc tccagcggca caacggtgcg ggtcgacggc
     9901 tacaccgaca acagcggcaa cgacgccatc aacaaccccc tgtcggtcgc ccgggcgcag
     9961 gcggtcgaga cgcagctcaa agccctggtc agccggcagg gcgtcagctt ccaggccgcc
    10021 ggccacggct ccgccgaccc ggtcgccgac aacggcagcg aggaggggcg ccgcaagaac
    10081 cgccgggtca ccgtgacgtt cgcccgccca gccccccaga cctcgtcagg atccgggtcc
    10141 gccgctccgg ctccggccgc tccagcaccg gcgtggaccg gcggcaagct gccggtggtc
    10201 gcgaccgcgg cgtccgtggg ggacaacgtg aagagcgtga agctggagat caacagcgtg
    10261 caccgcgacg cggccggcct caccaccatg acctggaccc tgaccaacac cggcaccgcc
    10321 aagttcggcg tcgggttcac cttcgtggac cgaggccagg agttcaacta cgacggcctg
    10381 aacgtccccg gcgccaggct ggtcgatcag gccggcaagc tgacctacaa ccccctccgc
    10441 gacgagggga aggagtgcgt ctgcacgaac ctcaacaagg gcaagggcga cctcgacccg
    10501 ggggagagcg tgacctactt cgacagctac gaactcccgc ccggagtcgc cagcgtcgac
    10561 atcaccttcc ccttcttcgc accggcccag cacgtccagg tgcaataggg cacttcaccc
    10621 gaggagcaca gcggtggtca aggacggagc cagcagcgag gcaaaaccat cgtcccaaaa
    10681 ggaaattcga gctggtcgac cggataccag gacagaggga agcgaaacct tcgaagcccg
    10741 aattctggcc ctccagaagc agggaaagac ctatgaggag gcgcttcaaa gcctcgagga
    10801 cggcgagtga cccaccccgc tcagcgcacc accaggaccg ggcggcgctc gcgacgcacc
    10861 agccgggtcg cggtcgagcc gccgaggagg atgcgcagca ggccggaacc gctgctgccg
    10921 accacgatca ggtcggcgtc gacggccgcg gcgagctggt cgatgcggtg gaccgggtcg
    10981 ccgttctcga cgcggaagcg ccagcggatg cccagcgggt cgagggtctc ggtgacccgg
    11041 atgccgacct cgtactccag gtcctcgcgc cactgctcga tcatcccgca cgaggcgggg
    11101 gtgaggccgc caaagaccgg cgcctcgggc cgcacgtgaa ggacggtgag ctgggccccg
    11161 gcgccgctgg cgatctggcc ggccagggcg accgcgtgca ggctcgcctg cgagccgtcg
    11221 gccgccacca ccacgttgcg gagcgacccg ccgacccgtt gcgaccaggc ccgaggatcc
    11281 ccacttggaa caggtctcgg cggcggctcc acggccatcg cccccgcccc cttttcccct
    11341 tcgtcgttga agttgaagct gcggaagcct taccacaatg gtcctaccca ctgacgaacg
    11401 gcgcaagcat gactgccgat ccagcagagg atcatggctc ccggaaggtc gtcaaccgga
    11461 acaccaacac ccgatgaagt tcctcgatcg cctggtaggc gagatagtag agcccgccac
    11521 cagcggtctc tacctcgaag gtataccagc cgtcctcgta ggagatcggg aagcgtccgc
    11581 tctcgttggt ggggtcttcc cgaagcaccg gatagacaac tcggccgagc gcatcaagga
    11641 acttagcgga tgcttgcttg tactggtcga gtgtgtctgg agtctcgaaa atgtgatagt
    11701 cggccactac tcgatgccgc gctcccgaag caccgtggca aggccgttct cgacgagccc
    11761 gagcgactcg ggtgactccc cacgcagcag cgccgtacgc aaccgccgtg ccagcgggtc
    11821 cagctcagcg agttgctgtg agatgacctt cctgacgtgc tcatggtcca cgggcggatc
    11881 acctgattcg tcaggcaaag caggacaact catgatggtc ccccctcacg accgcggaca
    11941 tactgtcggt tcggctgatg gtacccgatc acatggacat ctgtcccaag acgggcctct
    12001 cagtgttctg gaggctcgac gccggggacg tggcgggcgg cgaactcgag gatgacctcg
    12061 gtctggcgga tgcgttcgtc ggtgaccagg gcgccgtggc cggcgtcgaa gcggtcgagc
    12121 tcgaagggct tgcggaggga gcggagcgcg tcggcgtag
//
LOCUS       DASWDC010000239         4936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_199465, whole genome shotgun sequence.
ACCESSION   DASWDC010000239 DASWDC010000000
VERSION     DASWDC010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199465"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..1121
                     /locus_tag="VF995_07825"
     CDS             426..1121
                     /locus_tag="VF995_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9343505.1"
                     /translation="MPKVLVVDDEESLLDAIRYALSREGMEVVTARDGAEAISDFEAE
                     RPDLVVLDLMLPTQSGWDVCRRIRASSAVPILMLTARDAEVDRVVGLEMGADDYVTKP
                     FSLRELVARVRALLRRAGPYQGGPAQSSPGTVLEVGGIRLDQERHEVSVRGEPVSLPL
                     KEFELLEILMENRNRVLTRQMLIDRVWGFDYVGDTKTLDVHVKRLRARLEEDRHEPKL
                     IVTVRGVGYRFDG"
     gene            1249..1641
                     /locus_tag="VF995_07830"
     CDS             1249..1641
                     /locus_tag="VF995_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9343506.1"
                     /translation="MYEFRLDGRSGVPPYLQLVQQVKQALLLGYLQPGDQLPTVKDVA
                     AGLAINPNTVVKAYRQLEHDGLVVPRPGQGTFIARTLDAVEPGAYGALRRSLERWLRS
                     AYDAGLDEEAVNALFSTAHHELRAEGVA"
     gene            1644..2576
                     /locus_tag="VF995_07835"
     CDS             1644..2576
                     /locus_tag="VF995_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9343507.1"
                     /translation="MEATFALETERLGKRYGRPWALRACTFATPEGRVIALVGPNGAG
                     KTTLLQLVAGLLAPTEGTVRVFGEAARANTPAALARVGFVAQDHPLYKGFTVADLLTM
                     GRRLNLPGRFDQALAERRLADLGIPREQRAGKLSGGQQAQVALALALAKRPRLLLLDE
                     PLASLDPLARREFMQALMGAAAEDGLTVVLSSHVVAELERVCDHLVLLTSGRVRLAGE
                     VDELIASHRLLTGPRSDPAAAGQIPGVLRASHGDRQTTLLVRGDAGPERPAVHPNWQS
                     HPVTLEDLVLAYLQLRSEAAAAPNPPAEPVEVVR"
     gene            2576..3556
                     /locus_tag="VF995_07840"
     CDS             2576..3556
                     /locus_tag="VF995_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9343508.1"
                     /translation="MVWLTWRQHRAEALGGLLALAAVAASLLALGLPAHHAFAHEGIA
                     ACLAQASSGPVLTSGSCGEALKLFSGRYLNSGAGGLIPWLNFLPAAIGVFVGAPLLAR
                     EFEHGTWRLAWTQAVPRTRWLAVKLVLLVAGVAALTAVTAVLFTWYRGPFDQIDGRMW
                     ASAFDFEGLTIPAYALFAFALGILAGILTRRIVVSMAVTLGGFLAVRLPVEFWLRPHY
                     QRPVTMTVDPLSTQGPTRVDQVLSTNLIDSAGHTLSDLQTRRVMDAALQGATRGDVNT
                     YLQAHSYHWSYVFQPAARFWHFQLIEAAIFIGLAAALLTSAVWLLRRRVG"
     gene            3570..3722
                     /locus_tag="VF995_07845"
     CDS             3570..3722
                     /locus_tag="VF995_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018728744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9343509.1"
                     /translation="MLVRQRGSHRQYKHPIKPGLVTVPGNLGKDLSHGLVCSILKQAG
                     LLKGDR"
     gene            3719..3955
                     /locus_tag="VF995_07850"
     CDS             3719..3955
                     /locus_tag="VF995_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017625589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9343510.1"
                     /translation="MTVNVYTYLVVIERAEDGGYGAWAPDLPGCVAIGETLEECERQM
                     QEAVAFHVEGLRLEGEAVPEPSAVAATLVKVPAA"
     gene            complement(3936..>4936)
                     /locus_tag="VF995_07855"
     CDS             complement(3936..>4936)
                     /locus_tag="VF995_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX9343511.1"
                     /translation="KEGLALINGSPCAAALSADAVLSARARLALAYEVFALSAEAFRA
                     PLDAYDPALDALWGDEHEARALRKLRALLGMDSMDSVEGGRGMASPDGRRPYQAPVSY
                     RILPRVLGQAERVLDAAEHVATVSLRSVSDNPVYLPPSPNAPDPEHPDGRVLSTGGYH
                     NAAAPAALHDLTVCWAELCQLAERHVEHLTFTVSPPASRADGGELLLHLLLMVAVGYS
                     EEARAAAQPPLLPRAGPGQNDVTAPSFLAWSREQTAAASLDAALALVAAAAGHWLAGN
                     TLDPPPRLARLAAETREHFPPLTDRRSLGADVANLAAHFRARVFAEVAQPATPREP"
BASE COUNT          665 a         1791 c         1733 g          747 t
ORIGIN      
        1 cggccagcgc ctccagggtc cggtcgagct gcgactggac cacctggccc atctgcagca
       61 gcttcgcttc caggccgtcg aggcgttgct cgaaggtggt gcgcacgtcg tctcctgcct
      121 gttccatcgg ggggccccgc ggctactgga gcacaggctc gcggtgcttc cggccgcggg
      181 accatgcccc ccgaaccccc cgggggttcg ctgtcctgtt acctggacca atggtacgcc
      241 gtggctgtcg aactccctga gggtgacaag gtggccggag ggcgaacggg aggtgaacct
      301 tgcgcgcaag ccgcgcgagg cgagcccggc accttgccgg cctggacgaa gctgtggaag
      361 cgtacacagg gggctgccgc gcaggatggg gtcgctgagg cagtccgcgt cgaacggggt
      421 ggtccgtgcc gaaagtcttg gtggttgacg acgaggaatc gctgctcgac gcgatccgct
      481 acgcgctgtc gcgcgagggc atggaggtcg tgaccgcccg cgacggcgcc gaggcgatca
      541 gcgacttcga ggcggaacgc ccggatctgg tggtcctcga cctgatgctc ccgacccaga
      601 gcggctggga cgtctgccgc cgcatccgcg cctccagcgc cgtcccgatc ctgatgctga
      661 ccgcccgcga cgccgaggtc gaccgcgtgg tcggcctgga gatgggcgcc gacgactacg
      721 tcaccaagcc cttctccctg cgcgagctgg tcgcccgggt ccgcgccctc ctgcgccgcg
      781 ccgggcccta ccagggcggc cccgcccaga gcagccccgg gaccgtcctg gaggtcggcg
      841 gcatccgcct cgaccaggaa cgtcacgagg tctcggtccg cggcgagccg gtcagcctcc
      901 cgctcaagga attcgagctg ctcgagatcc tgatggagaa ccgcaaccgg gtcctcaccc
      961 gccagatgct gatcgaccgc gtctggggct tcgactacgt gggcgacacc aagaccctcg
     1021 acgtccacgt caagcgcctc cgcgcccgcc tcgaagagga ccgccacgaa cccaagctga
     1081 tcgtcaccgt ccgcggcgtg ggctaccgct tcgacggctg accctgacgg gtcccgggtt
     1141 caccacgcga gctgccctcc ttcgggctgg ggcttgtgcc ttctcaactt ctactgtttt
     1201 actatgatca tagtagaaca gtagaggtcg ggaaggagcc tgccaccgat gtatgaattc
     1261 cgcctggacg gccgctcggg cgtgccgccg tacctgcagc tcgtgcagca ggtgaagcag
     1321 gccctgctgc tcggctacct gcagcccggg gaccagctcc ccaccgtcaa ggacgttgcc
     1381 gcgggcctgg cgatcaaccc caacacggtg gtcaaggcct accggcagct tgagcacgac
     1441 ggcctggtgg tgccccgacc tgggcagggg accttcatcg cccgcacgct cgacgctgtc
     1501 gagccgggcg cctacggagc gctgcgccgc tcgctggagc gctggctgcg cagcgcctac
     1561 gacgccgggc tcgacgagga ggcggtcaac gccctttttt ccaccgccca ccacgagttg
     1621 cgcgctgagg gggtggcata gccgtggaag ccaccttcgc gctcgagacc gagcggctcg
     1681 gcaagcgcta cggccggccg tgggcgctcc gtgcctgcac cttcgccacg ccggagggca
     1741 gggtcatcgc cctcgtcggg cccaacggcg ccggcaagac caccctgctc cagctcgtcg
     1801 ccgggctgtt ggcgccgacc gaggggaccg tccgggtgtt cggcgaggcg gcccgcgcca
     1861 acaccccggc cgcgctcgcc cgggtcgggt tcgtcgccca ggaccacccc ctctacaagg
     1921 gcttcaccgt cgccgatctg ctcaccatgg gccgccggct gaacctgccg gggcgcttcg
     1981 accaggcgct ggccgagcgg cgcctggccg acctcggcat cccccgcgag cagcgcgccg
     2041 gcaagctctc cggcggccag caggcccagg tcgcgctcgc gcttgcgctg gccaagcggc
     2101 cacggctgtt gctgctcgac gagccgctgg ccagcctcga cccgctcgcc cgccgcgagt
     2161 tcatgcaggc gctgatgggc gccgcggccg aggacggcct caccgtggtg ctctcctccc
     2221 acgtcgtcgc cgagctggag cgggtctgcg accacctggt cctgctcacc tccggccggg
     2281 tccggctcgc cggcgaggtc gacgagctga tcgccagcca ccgcctcctg accggcccgc
     2341 gcagcgatcc ggcggccgcc ggccagatcc ccggcgtgct gcgagccagc cacggcgacc
     2401 gccagacgac cctgctggtc cgcggcgatg ctgggccgga gcgccccgcc gtccacccca
     2461 actggcagtc ccaccccgtc accttggagg acctcgtgct cgcctacctc cagctccgga
     2521 gcgaggccgc cgccgcgccg aacccaccag ccgaacctgt ggaggtggtg cgctgatggt
     2581 ctggctcacc tggcgccagc accgcgctga ggccctcggt ggcctcctcg cgctcgcggc
     2641 agtcgccgcg agcctgctcg ccctcggcct gccggcgcac cacgccttcg cccacgaagg
     2701 catcgccgcc tgtctcgctc aggccagctc cggccctgtc ctcaccagtg gcagctgcgg
     2761 cgaggcgctc aagctgttca gcgggcggta cctcaacagc ggagccgggg ggctgatccc
     2821 ctggctcaac ttcctgccgg cggccatcgg cgtgttcgtc ggcgccccgc tgctggctcg
     2881 ggagttcgag cacggcacct ggcggcttgc ctggacacag gccgtgccgc gcacccgctg
     2941 gctcgccgtc aagctcgtcc tgctggtcgc gggcgtcgcc gcgctgacgg cggtgaccgc
     3001 cgtcctcttc acttggtacc gcgggccgtt cgaccagatc gacgggcgca tgtgggccag
     3061 cgcgttcgac ttcgagggcc tcaccattcc cgcctacgcg ctgttcgcct tcgcgctcgg
     3121 catcctcgcc ggcatcctca cccgccgcat cgtggtctct atggccgtta ccctgggcgg
     3181 cttcctcgcc gtccgcctgc cggtcgagtt ctggctgcgg ccccactacc agcgccctgt
     3241 cacgatgacc gtcgacccgc tctccaccca ggggccgacg cgcgtcgacc aggtcctcag
     3301 caccaacctg atcgactccg ccggccacac cctcagcgac ctccagaccc gccgagtgat
     3361 ggacgccgcc ctccagggag ccacccgcgg cgacgtcaac acctacctgc aggcccacag
     3421 ctaccactgg agctatgtct tccagcccgc cgcccgcttc tggcacttcc agctcatcga
     3481 agccgccatc ttcatcggct tggccgcggc cctcctcacc agcgccgtct ggctactccg
     3541 ccgccgcgtc ggttgaccga tatggctgga tgctggtccg ccagcgtggc agccatcgac
     3601 aatataaaca tccgatcaag ccggggcttg tcaccgtccc tggcaacctt ggtaaggact
     3661 tgtcgcacgg cttggtgtgc agcatcctca agcaagctgg actgttgaag ggtgatcgat
     3721 gactgtgaat gtctatacct accttgtcgt gatcgagcgt gccgaggatg gcggctacgg
     3781 cgcttgggcg cctgatctgc ctggatgtgt ggcgattggg gagacgctcg aggagtgcga
     3841 acggcagatg caggaggcgg tcgccttcca cgtcgaaggg ttgcgtttgg agggcgaggc
     3901 ggtccctgaa ccttcggcgg ttgccgccac gctggtcaag gttcccgcgg cgtagctggc
     3961 tgggcgactt ctgcgaacac gcgggcgcgg aagtgcgctg ccaggttggc gacgtcggcg
     4021 ccgagagagc ggcggtcggt gagcgggggg aagtgctcgc gggtctcggc ggcgaggcgg
     4081 gcgagccgcg ggggcgggtc cagggtgttg ccggcaagcc agtggccggc ggcggcggcc
     4141 acgagggcga gcgcggcgtc gaggctggcg gcggcggtct gttcgcggga ccaggcgagg
     4201 aaggaggggg cggtgacgtc gttctggccg ggcccggcgc gggggagcag cggcggctgg
     4261 gcggcggcgc gggcttcctc ggaatagccg accgcgacca tcagcagcag gtggagcagc
     4321 agctcgccgc cgtcggcgcg gctggctggg ggggagacgg tgaaggtcag gtgctcgacg
     4381 tggcgttcgg cgagctggca cagctccgcc cagcagaccg tcaggtcatg gagggcggcg
     4441 ggggcggcgg cgttgtggta gccgccggtg gagaggacgc ggccgtcggg atgctccggg
     4501 tccggtgcgt tgggtgatgg cggcaggtac accgggttgt ccgacactga gcgcagcgac
     4561 acggtggcga cgtgctcggc ggcgtccagc acccgttcgg cctggccgag tacgcgcggg
     4621 aggatgcggt agctgaccgg tgcctggtag ggccggcgcc cgtccgggct ggccatcccc
     4681 cgcccgccct ccacactgtc catactgtcc atgcccagca gcgcccgcag cttccgcagc
     4741 gcccgcgcct cgtgctcgtc accccagagg gcgtccaggg ccgggtcgta ggcgtccagc
     4801 ggcgcgcgga acgcctcggc cgagagcgcg aagacctcat aggcgagcgc cagccgggcg
     4861 cgggctgaaa gcaccgcgtc ggcgctcagg gcggcggcgc agggggagcc gttgatcagc
     4921 gccaggccct ccttct
//
LOCUS       DASWDC010000240        14143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_894653, whole genome shotgun sequence.
ACCESSION   DASWDC010000240 DASWDC010000000
VERSION     DASWDC010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14143
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894653"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VF995_07860"
     CDS             complement(<1..199)
                     /locus_tag="VF995_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Nuclear pore complex protein-Nup96 precursor"
                     /protein_id="HEX9343512.1"
                     /translation="MRPRTIGTAAGLVGLALLVGLAASSCTAGGKTASSSDAARPAIG
                     APVGSAGGGSAASAGAGSTAES"
     gene            401..2779
                     /locus_tag="VF995_07865"
     CDS             401..2779
                     /locus_tag="VF995_07865"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX9343513.1"
                     /translation="MAVAVSPTTTTTTTGTRRGLVLGLFVASGAAGLIYQVVWSRELV
                     LVFGNTTQAVATIVTAFMAGLGFGSLVGGRRASRTANPLALYGMVELAVAVYALLLPF
                     AFASLGDLYRGVYPSLVEHPAGLTAVRLLLALAAVAPATFLMGMTLPLLVRHLVRTLD
                     EAGARLGELYAANTGGAFTGTLLAGFVLIEFLGLRRTSYVAVALNLLAGLGAQFLARR
                     DRERDRNRVAAGGGEAGGVARPEPRSAAVVDLTGPERLRRRLVLAATFVSGFVALALE
                     VLWTRMLAAGTGVSIYIFATILAIYLLGITLGSAAYRRWTRPGWERLAVLGLCLGAIG
                     LLAQLTVVLGSGVAGSPSFLVRSVVILLPATALMGFAFPLAGRLLVPSAERAGASVGL
                     LYAANTAGSILGSFSAAFILAGTLGTNGSVLLLGGLNLLTGAALLLAGSTESATGRGR
                     TLVGRPVAAALIVLAVGGLVASSLDLPVTRTATENRLRATGLPVTHAEDELATVDTLG
                     GPAAGRRLLVGGVGMTSLTVDTKLMAYLSKALRPQARDFLVIAFGMGGTFRAGLNAGF
                     QTDAVELSPTVPSRMPVFFPDAERWLHDPDGHVIVSDGRNYVRLTRKHYDVIAADPPP
                     PVESAGSVVLYTREFLTEGKARLNPGGVFMLWVPYSVPLEDLKTHVRTFHAVFPHVSL
                     LLAPARHGVYMFGSEAPLTFDDQSLLNVFRDPVVRGDLTGSPDYRPTDPAGWVRATHH
                     AEWLSDDQVSAFTGPGPLITDDQPRSEYYFWRRAKMADRRYINEAMLRAAAP"
     gene            complement(2872..4254)
                     /gene="dnaB"
                     /locus_tag="VF995_07870"
     CDS             complement(2872..4254)
                     /gene="dnaB"
                     /locus_tag="VF995_07870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012880089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX9343514.1"
                     /translation="MAIRPPVAEGGGSRASASPAYERIPPHNIEAEESVLGSMLLSRD
                     AIAEVLELLHEDDFYRPAHRTVFNAILGLYSHGQAVDAVTVSEELRRGGQLADVGGAP
                     FLHTLLAGVPTAANAAYYARIVKEAGTMRRLIDVGTRIVQLGFETPQDTERAVDTAES
                     LVYQVAQGRVADDYQSLREVLTSTLEAIERLHDDHREITGVPTGFPDLDRVTSGLQPS
                     NLIIVAARPAVGKSTLGLDIVRNASVKANVTSVIFSLEMSKSELVQRLMCAECSVDMQ
                     RLRTGRMDESDWTRLTRSLGRLADAPLFIDDSASTTLMEIRAKCRRLKQRHGLGLVVV
                     DYLQLMQASRKVDNRQQEVSEMSRGLKLLAKELAVPVIAISQLSRNPEARSDKKPMLS
                     DLRESGSLEQDADCVLFIYRDEIYDPESPRKGEADLILAKHRNGPTDTVVVTFQGQYS
                     RFAPMAARSL"
     gene            complement(4300..4746)
                     /gene="rplI"
                     /locus_tag="VF995_07875"
     CDS             complement(4300..4746)
                     /gene="rplI"
                     /locus_tag="VF995_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017595521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX9343515.1"
                     /translation="MKVILNQEVRGLGAPGDIAEVADGYGRNYLLPRNLARLATPGAV
                     RQADGIRARRIAREIANLEQAQTIATHLESLRVVIPAKAGKEGRLFGSITTPQIVEAV
                     QRTGGVEIDRRRIHLDNPIKQTGTHQLHVRLHPEVEATVNLEVVPG"
     gene            complement(4786..5019)
                     /gene="rpsR"
                     /locus_tag="VF995_07880"
     CDS             complement(4786..5019)
                     /gene="rpsR"
                     /locus_tag="VF995_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012808120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX9343516.1"
                     /translation="MPKSTFQRKPKRKFCQFCAEKQDFIDYKDVNLLRKFMSDRGKIR
                     ARRVSGNCSQHQRDVATAIKNAREMALLPYSIR"
     gene            complement(5092..5553)
                     /locus_tag="VF995_07885"
     CDS             complement(5092..5553)
                     /locus_tag="VF995_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX9343517.1"
                     /translation="MANDNQVMVVGNLTDDPELRFTANGAAVANFRLAVTPRMREGDQ
                     WKDGETSFFRVNVWRQQAENVAETLTKGSRCIVVGRLRMRNWETPEGEKRSVTEIEAD
                     EIGPSLKFATAKVERSSRGGGDFAGSSAGSSAGSSAGGSRGGGQFSDEPPF"
     gene            complement(5701..5988)
                     /gene="rpsF"
                     /locus_tag="VF995_07890"
     CDS             complement(5701..5988)
                     /gene="rpsF"
                     /locus_tag="VF995_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX9343518.1"
                     /translation="MRNYEVMIILDPSLEDKDAKAAVDKFLTTVTTRGGTVRNIDHWG
                     KRRFAYEIRHITEGYYTVVELEAEAATVDELSRVLGLADGVIRHKVIRPAA"
     gene            complement(6232..7521)
                     /locus_tag="VF995_07895"
     CDS             complement(6232..7521)
                     /locus_tag="VF995_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343519.1"
                     /translation="MPAVPAAVANPPVEQVPSTRWTATPLLWLWVATRVLLLIVSLNP
                     RLYSGGLFGDVRTYGARVERMFQGELPYRDIAIEYPPGSVPFTVIPGLVTGAGAGYRL
                     AFAVEMLLVDAVGLYAAMRLARAADAARRRIPLAYVLLMFAVGPLLVLRFDLVPAVCI
                     LLAAALAAEGSAGWAAAALGFGTAAKLFPLVLAPLLVLGLVPAQGWGWWRTLRRTVPP
                     FLLGFAVTVVPALLLSVHGTLNAVNYQTARGVQIESLPANAIDLVHLATGLATRSFLD
                     FGAYHLASSLSAPVKTISVAATAATLVAAVALVGWCSWRRGGLRPRDWAPAFLIGVFA
                     FMLPARVLSPQYLVWIAALLAGLADRRRARPALLALVAAAAATQVIYPFRYPQLIRFD
                     AVDIGLLTVRNLLLVAITAVVVWTFVRPGPEAAPNPS"
     gene            complement(7531..9696)
                     /locus_tag="VF995_07900"
     CDS             complement(7531..9696)
                     /locus_tag="VF995_07900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX9343520.1"
                     /translation="MATRTTVPSRTARLHTAPQRPRTKRILFWVLWLTVPVIAILFGV
                     VIGVVYAFAKLPVTNQAPPAQSISFSDANGAPLGTISPIANRQVVDFKNIPPVMREAV
                     LAAEDRDFYKHGALSYKGLLRAAWSNVVQRRVAEGGSTITQQYVKNVFPAVGNERTMF
                     RKVKEAVIAIKLEHHYTKDQILGSYLNAVYFGHGAYGVDAAAHAFFGKSVEKLTPAQA
                     ATLAGAIRAPEVYTRKPNARIAKERRNFILGVMQQRGWLSADAAAKAEAAPIAENWTV
                     HASGIANSKAPFFLDLVRQYLVDQFGAAAVAQGGFSVRTTLDLKMQAQAEAAVKGILN
                     QQGDPPASLVALDPKTGAVRAIYAGASAKQWATHQFDYATDPPGRQAGSTFKPFVLEQ
                     ALLDGVTLDQPLLAPDKLLIPTGKPKPDDVQTIRNFAGEHFSGKISLLQATTQSVNTA
                     YTMLLNQLARNGQSGPDRVAALAHRTGLDTTLNSAEHKPNLAHVPSLALGPSDVTTLQ
                     LASAFGTWANQGVHQEPFLVEWIKDAKGHLIKLPPHRSETVLDANTANTMSFALRSVV
                     EHGTGTAARLPDRQVAGKTGTTNDSKDARFVGYTTDLVTSVWMGYTTPKPMVNLHGVA
                     NVTGGSLPAEIWHNFMAAATQGTPGTAFQLPQQASVLPAPATTVAPAPTTPTTEVPTS
                     LPPSTLPPSSSPTSSTLPTSTTFGFATSTTRFRPTTTTR"
     gene            complement(9798..10157)
                     /locus_tag="VF995_07905"
     CDS             complement(9798..10157)
                     /locus_tag="VF995_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HEX9343521.1"
                     /translation="MRLVDYTLAKRAKLRELRFGGLTRGDVCDAHPELLRAARNVGES
                     TRTPCPVCAETCLTHVTYVFGDALRQESGRVWPSAALADLHRTMDEFACYVVEVCTGC
                     SWNHLAQHYLLGRRHAG"
     gene            10270..10893
                     /locus_tag="VF995_07910"
     CDS             10270..10893
                     /locus_tag="VF995_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9343522.1"
                     /translation="MLELAVLGLLKESPMSGYELKKRLTVTHGAFWRVSFGSLYPCLK
                     RLLADGALAVEESSQFTSRKKHVYRLTEHGERLFQEMLESTDAHDLEQDRFSLRLAFF
                     RYLGPESRLDQLERRRGYLQERLADLRASLRTVRDRLDAYTIELMDHGLDAHEREIAW
                     LDRLIAAERHSTSAAAAASAPQPPPLPAPPPDQKPATTPARTEGVKT"
     gene            10893..11966
                     /locus_tag="VF995_07915"
     CDS             10893..11966
                     /locus_tag="VF995_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX9343523.1"
                     /translation="MAKVRVAIVGVGNCASSLVQGVQYYQDALEGDDVPGLMHVELGG
                     YHVRDIEFVAAFDVDAKKVGLDLAEAVLASENNTIHLADVPPLGVEVRRGPTLDGLGR
                     YYQEMITESEQAPVDVAAALRDARADVLVCYLPVGSEEAARSYAAQAIEAGVAFVNCL
                     PAFIATDPAWAERFRAAGLPIIGDDIKSQVGATIVHRVLSRLMEDRGITIDRTYQLNF
                     GGNMDFMNMLERDRLQSKKISKTRAVTSQLEHDLGKRNVHVGPSDHVPWLDDRKWCYI
                     RIEGRAFGDVPLNLELKLEVWDSPNSAGVVIDAIRCAKLGLDRGLAGPLLGPASYFMK
                     SPPVQYTDDVARQAVEDFIANKT"
     gene            12198..12794
                     /locus_tag="VF995_07920"
     CDS             12198..12794
                     /locus_tag="VF995_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343524.1"
                     /translation="MPSTTNRRPLRRLLLVPVLGVAVLSSVAFSGIAYAATPQAATAP
                     AQPSADKPATDSSSGSSSADNSSSSAPSNPLDIVLNVLKPVLALVQGLPDQLLGALGT
                     SGSSSSDNSTTTTTSGSSAGTAKTEVAIPEGTQVIAACQEPSADAYTVHAALPQAVSG
                     LQEGTSIQLDSSALTGLAGQAVPNVPMCASSDSMISNS"
     gene            complement(12801..>14143)
                     /locus_tag="VF995_07925"
     CDS             complement(12801..>14143)
                     /locus_tag="VF995_07925"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX9343525.1"
                     /translation="GHQLYLVGGTVRDALLERPSPDVDFTTDATPDEILALVRAGHWH
                     DGVWLQGVRFGTVGVRKGDLRLEVTTFRSDIYRDDSRKPEVTFGTTIEEDLARRDFTV
                     NAMAVHLPQGQFVDPFGGLADLAARCLRTPGDPVGSFEDDPLRMLRAARFVSQLELQP
                     TRETLAAIGERVERLDIVSRERIRDELSKLLCGRAPGNGLWLTVNSGLAARFLPELPA
                     LALEQDPVHRHKDVLAHTIAVVERATAFDQDEPDLTLRMAALLHDIGKPRTRRVGSDG
                     VSFHLHEVVGAKMAERRLTELRYAKDFIQDVRRLVELHLRFHTYRLGWTDSAVRRYVR
                     DAGPLFDRLNFLVRSDCTTRNALKARQLAAACDELEERIARLAAEEELASIKPPLDGR
                     QVMEILGITPGPLVGQALAHLLELRLDRGQMTNEEAVAALHDWARDHGLPGVLK"
BASE COUNT         2036 a         4821 c         5103 g         2183 t
ORIGIN      
        1 cggattcagc ggtggaaccc gcgcccgcgc tggccgcgct cccacccccg gcgcttccca
       61 ccggggcgcc aatggctggc ctggccgcgt cactcgacga cgccgtcttg ccgccagcgg
      121 tgcagctgga agccgccagt cccaccagca gcgcgagccc caccagcccg gccgctgtcc
      181 cgatcgtccg cggcctcaca ccgctgctcc tgcctgtgcc catgcccgac ctcccggtct
      241 cgacacccgc cgcgcttgcc ttgcgtccgc ctctttgagg gcggggaagg cccgccggtt
      301 cccggcccct ggcccggtgc gtgcttgagg tcgcttgccc tcgggcacga acttctgcga
      361 gccttccggc ctgcacagtt cgccaaccag ggagccgccc gtggctgtcg ccgtctcgcc
      421 caccacgacc acaaccacca ccggcacccg ccggggactc gtcctcggcc tgttcgtcgc
      481 ctccggcgcc gccgggttga tctaccaggt cgtttggtcc cgcgagctgg tgctggtgtt
      541 cggcaacacc acccaggcgg tggcgacgat cgtgaccgcg ttcatggccg gcctcggctt
      601 cggcagcctg gtcggcgggc gccgtgccag ccgcaccgcc aacccgctcg ccctctacgg
      661 gatggtcgaa ctggccgtgg cggtctacgc gctgctgctg ccgttcgcct tcgcgagcct
      721 cggcgacctc taccgggggg tctatcccag cctggtggaa cacccggccg gactgaccgc
      781 ggtgcggctg ctgctcgcgc tcgcggcggt cgcccccgcc accttcctga tgggcatgac
      841 cctgccgctg ctggtccgcc acctggtccg caccctggac gaggcggggg cacgcctcgg
      901 cgagctgtac gcggccaaca ccggcggcgc gttcaccggc acgctgctgg ccggcttcgt
      961 gctgatcgag ttcctcggcc tgcgccgcac cagctatgtc gcggtggcgc tgaacctgct
     1021 cgccggcctg ggcgcccagt tcctcgcccg ccgcgaccgg gaacgcgaca ggaaccgggt
     1081 cgccgcgggt ggaggcgaag cggggggagt ggcgcgtccg gaaccgaggt ccgccgcggt
     1141 tgttgacctc accgggccgg agcggttgcg gcggcggctg gtgctggcgg ccaccttcgt
     1201 gtcgggcttt gtggcgctcg cgctcgaggt gctgtggacc aggatgctcg ccgcgggaac
     1261 cggggtgagc atctacatct tcgccaccat cctggcgatc tacctgctcg gcatcacgct
     1321 cggcagcgcc gcctaccggc gctggacacg tcccgggtgg gagcggctgg cggtgctcgg
     1381 gctctgcctc ggcgcgatcg gcctgctcgc ccagctcacc gtggtgctcg gcagcggcgt
     1441 ggccgggagc ccgtcgttcc tggtccgctc ggtggtgatc ctgctgccgg cgaccgccct
     1501 gatgggcttc gccttcccgc tcgccgggcg gctgctggtg ccgtcggcgg agcgcgcggg
     1561 cgccagcgtc ggcctgctgt acgcggccaa caccgccggc tcgatcctcg ggtcgttcag
     1621 cgccgcgttc atcctcgcgg gcaccctcgg gaccaacggc tcggtgctgc tgctcggcgg
     1681 cctcaatctg ctcaccggtg cggccctgct gctggcgggg tccacggaga gcgccacagg
     1741 gagagggcgg acgctggtgg gacggccggt ggcggccgcg ctcatcgtgc tcgcggtcgg
     1801 cgggctggtc gcctcctccc tggacctgcc ggtcacccgc accgcgaccg agaaccggct
     1861 gcgcgcgacc gggctgccgg tcacccacgc cgaggacgag ctcgccaccg tcgacacgct
     1921 cggcgggcca gcggccggac ggcggctgct ggtcggcggg gtgggcatga cctcgctcac
     1981 cgtcgacacc aagctgatgg catacctgtc caaggcgctg cgtccccagg cgcgggactt
     2041 cctggtgatc gcgttcggca tgggcggcac cttccgcgcc gggctgaacg ccggcttcca
     2101 gaccgacgcg gtcgagctgt cgccgacggt gccgagccgc atgccggtgt tcttccccga
     2161 cgcggagcgc tggctccacg accccgacgg ccacgtgatc gtcagcgacg gacgcaacta
     2221 cgtgcgcctc acccgcaagc actacgacgt gatcgccgcc gacccgccgc cgccggtcga
     2281 gagcgccggc tcggtggtgc tctacacccg cgagttcctc accgagggca aggcgcgcct
     2341 caacccgggc ggcgtgttca tgctctgggt cccctactcg gtgccgctgg aggacctgaa
     2401 gacccacgtg cgcaccttcc acgcggtctt ccctcacgtc agcctgctgc tcgccccggc
     2461 ccgccacggc gtctacatgt tcggctccga ggcgccgctg accttcgacg accagtcgct
     2521 cctcaacgtc ttccgcgacc cggtcgtccg gggcgacctg accggctccc ccgactaccg
     2581 gccgaccgac ccagccggct gggtccgcgc cacccaccac gccgagtggc tctccgacga
     2641 ccaggtgagt gccttcaccg gcccgggtcc cctgatcacc gacgatcagc cccgctcgga
     2701 gtactacttc tggcgccgtg ccaagatggc cgaccgccgc tatatcaacg aggcgatgct
     2761 gcgcgccgcc gccccgtagg ggtcgccgcc gagccacgcg cgcccaccgc tccgctcgct
     2821 ccgggtccga gttccgctgc ccctccccct cccccggcgc acacgccgct cctacaaact
     2881 ccgagccgcc atcggagcaa accgcgaata ctgcccctgg aacgtaacca ccacggtgtc
     2941 ggttgggccg ttgcggtgct tggccaggat caggtccgcc tcccccttac gcggcgactc
     3001 cgggtcgtag atctcgtcgc ggtagatgaa caggacacag tctgcgtcct gttcgagcga
     3061 accactttcc ctcaaatcgg acagcatagg ctttttgtcc gatcttgctt cggggtttcg
     3121 agagagctgc gagatcgcga tcaccgggac tgccagctcc ttcgcgagga gcttgaggcc
     3181 gcgggacatt tcgctgacct cctgctggcg gttgtcgacc ttcctgcttg cctgcatcag
     3241 ttggaggtag tcgaccacga ccaggccaag accatgacgt tgcttcagac gtcggcattt
     3301 cgcacggatt tccatcaacg tggtcgaggc agaatcgtca atgaacagcg gcgcgtccgc
     3361 gagcctcccg agagaacgtg tcagccgggt ccagtcggat tcatccatcc gcccggtccg
     3421 cagccgctgc atgtccaccg agcactccgc gcacatcagc ctctgcacca actcgctctt
     3481 actcatttcc agcgagaaga tcactgatgt cacgtttgcc ttgaccgatg catttcgtac
     3541 gatatccagc cccaaggtcg atttgcccac cgcgggcctg gccgcgacga tgatgaggtt
     3601 ggagggttgg aggccggagg tgacgcggtc gaggtcgggg aagccggtgg ggacgccggt
     3661 gatttcgcgg tggtcgtcat ggaggcgttc gatggcttcg agggtgctgg tgaggacctc
     3721 gcggagggac tggtagtcgt cggcgacgcg gccttgggcg acctggtaga cgagggattc
     3781 ggcggtgtcg acggcgcgct cggtgtcctg gggcgtctcg aaaccgagtt ggacgatgcg
     3841 ggtgccgacg tcgatcaggc gccgcatcgt gccggcttcc ttcacgatgc gggcgtagta
     3901 ggcggcattg gcggcggtgg ggacgccggc gagcagggtg tggaggaagg gggcgccgcc
     3961 gacgtcggca agctgtccgc cgcggcgcag ttcctcggag acggtgacgg cgtcgacggc
     4021 ctgaccatgg ctgtagaggc cgaggatggc gttgaagacg gtgcggtggg ccgggcggta
     4081 gaagtcgtcc tcgtggagga gttcgaggac ctcggcgata gcgtcgcggg aaaggagcat
     4141 cgagccgagg acggattcct cggcttcgat gttgtgaggc gggatgcgct cgtaggctgg
     4201 ggaagcgctg gcccgcgagc ccccgccctc ggcgaccggt gggcggatgg ccatggtgct
     4261 ctccttggtc ggtggtgccg cggcccgtgt gcgctggcgc tagcccggga cgacctccag
     4321 gttgacggtc gcttcgacct cggggtggag gcggacgtgg agctggtggg tgccggtctg
     4381 cttgatgggg ttgtcgaggt ggatgcggcg gcggtcgatc tcgaccccgc cggtgcgctg
     4441 gaccgcctca acgatctggg gcgtggtgat ggagccgaac aggcggcctt ccttgcccgc
     4501 cttggcgggg atcaccacgc ggagggactc gaggtgggtg gcgatggtct gcgcctgctc
     4561 gaggttggcg atctctcggg cgatgcggcg cgcgcggatg ccgtcggcct gccggaccgc
     4621 gcccggggtg gcgagccggg cgaggttgcg gggcagcagg tagttgcggc cgtagccgtc
     4681 ggcgacctcg gcaatgtccc cgggggcgcc gagcccgcgg acctcctggt tgagaatgac
     4741 cttcacgtcg ggaacctcgt ctgtgtctgg gtcgcttccg tgtcgctagc gaatcgagta
     4801 ggggagcagg gccatctcgc gggcgttctt gatcgccgtg gcgacgtcgc gctggtgctg
     4861 cgagcagttg ccgctgaccc gccgggcgcg gatcttgccc cggtcgctca tgaacttgcg
     4921 cagcaggttg acgtccttgt agtcgatgaa gtcctgcttc tcggcgcaga actggcagaa
     4981 cttgcgcttc ggcttgcgct gaaacgtgct cttcggcatt cgcatctatc cttcgtctct
     5041 taccggggca ctccgatcca ccgaagtacc ccggacctcg cttcggctcg cctagaaggg
     5101 cggctcgtcg ctgaactggc cgccgccccg ggacccgccg gcggagctgc cggcggagct
     5161 gccggcggag ctgccggcga agtcaccgcc gccccgggaa gagcgttcga ccttggcggt
     5221 ggcgaacttc aggctggggc cgatctcgtc ggcctcgatc tcggtgacgg agcgcttctc
     5281 gccctctggg gtttcccagt tgcgcatgcg caggcggccg acgacgatgc agcgggagcc
     5341 cttcgtcagg gtctcagcca cgttctcggc ctgctggcgc cagacgttca cgcggaagaa
     5401 cgaggtctcg ccgtccttcc actggtcgcc ctcacgcatg cggggggtga ccgccaggcg
     5461 gaagttcgcc acggccgccc cgttggcggt gaagcgcagc tcggggtcgt cggtcaggtt
     5521 gccgaccacc atcacttggt tgtcgtttgc cactggggat ccacctcact cacatcccgc
     5581 gccggccttg ggccgggcac gatccggtat gacggcccaa tcgatcgaga ccgatcggga
     5641 tcgggccagc ggggaaggga gacgccactt gggtcacggg gtcgccgtgc gcgcggcggt
     5701 tcaggccgcc ggacggatca ccttgtgccg gataaccccg tcggcaaggc cgagcactcg
     5761 cgagagctcg tcgaccgtgg cggcctcggc ctccagctcg acgaccgtgt agtacccctc
     5821 ggtgatgtgc cggatctcgt aggcgaaccg gcgctttccc cagtggtcga tgttccgcac
     5881 cgtgccaccg cgggtggtca ccgtggtcag gaacttgtcg accgccgcct tggcgtcttt
     5941 gtcctccagg ctggggtcga ggatgatcat cacttcgtag ttgcgcattg acgcatgtca
     6001 cctcctctgg actatggcgg cccccgcacg tgggcgagag caggagtgtg aagtcgagcg
     6061 agggtagcag cgcggtgccg ttgaaggcaa cgagccagag cccgctgctt cccgcgagcc
     6121 cgacgatgac cacgacgatc ccatccatgc gtgcagtctg acatggaccc ccgacagtcg
     6181 tcgttcgttg tccacagcct ctgaccaggg aaacaagcac cgcgcgggtc attacgaagg
     6241 attcggcgcc gcttctggcc ccggcctgac gaaggtccac accaccacgg cggtgatggc
     6301 gaccagcagc aggttgcgga cggtgagcag gccgatgtcg accgcgtcga agcggatcag
     6361 ctgcgggtag cggaacggat agatcacctg ggtggccgcg gcggcggcga ccagggccag
     6421 cagggccggc cgggcgcggc ggcggtccgc cagaccggcc agcagcgcgg cgatccacac
     6481 caggtactgc ggcgacagca cccgggctgg cagcatgaag gcgaacacgc cgatcaggaa
     6541 ggcgggcgcc cagtcccgcg ggcgcaggcc gccccgccgc caggagcacc agccgaccag
     6601 cgcgacggcg gcgaccaggg tggctgctgt ggcggcaacc gagatggtct tgaccggcgc
     6661 cgacaggctc gacgccagat ggtaggcgcc gaagtcgagg aacgaccggg tcgccagccc
     6721 ggtggccagg tggacgaggt cgatcgcgtt ggcgggcagg ctctcgatct gcacaccccg
     6781 ggccgtctgg tagttgaccg cgttgagggt gccatgcacc gacagcagca gtgccggcac
     6841 cacggtcacg gcgaacccga gcaggaaggg cggtacggtg cgccgcagcg tccgccacca
     6901 cccccagccc tgtgccggca ccaggccgag caccagcagg ggagcgagca ccagggggaa
     6961 cagcttggcc gcggtgccga agccgagcgc ggcggcggcc cacccggcgc tgccctcggc
     7021 cgccagcgcg gctgccagca ggatgcagac cgccggcacc aggtcgaagc ggagcaccag
     7081 cagcggcccc acggcgaaca tcagcaggac gtaggcgagc gggatgcggc gccgggcggc
     7141 gtcggcggcg cgggcgagcc gcatcgcggc gtagagacca accgcgtcga ccagcagcat
     7201 ctccaccgcg aaggccaggc ggtacccggc cccggctccg gtgaccaggc ccgggatgac
     7261 ggtgaacggc acgctgcccg gcgggtattc gatggcgatg tcgcggtagg gcagctcgcc
     7321 ctggaacatc cgctcgaccc gggcgccgta ggtgcggacg tcgccgaaca ggccccccga
     7381 gtacagccgc gggttcaggg agacgatcag cagcagcacc ctggtcgcca cccagagcca
     7441 caggagcggc gtggcggtcc aacgagtgga cgggacctgt tccacaggcg gattggcgac
     7501 ggcggcgggc acggcgggca tgggacgcgc ctatcgcgtg gtggtggttg ggcggaacct
     7561 cgtggtcgac gtcgcgaagc cgaaggtggt gctggtcggc aaggtgctgg aggtggggga
     7621 ggacgagggc gggagggtgc tcggcggcag gctggtcggc acctccgtcg tcggcgtcgt
     7681 cggcgcggga gccacggtgg tcgcgggggc cggcaggacg ctcgcctgct gcggcagctg
     7741 gaaggcggtg ccgggcgtgc cctgggtcgc cgccgccatg aagttgtgcc agatctcggc
     7801 gggaagcgaa ccgccggtga cattcgccac cccatgcagg ttcaccatcg gtttcggggt
     7861 ggtgtacccc atccacaccg aggtgaccag gtcggtggtg tagccgacga agcgggcgtc
     7921 cttgctgtcg ttggtcgtgc cggtcttgcc ggcgacctgg cggtcgggca gccgggcggc
     7981 ggtgccggtg ccgtgctcga ccacgctgcg cagcgcgaag ctcatggtat tggcggtgtt
     8041 ggcgtcgagc accgtctcgg agcggtgcgg cggcagcttg atcaggtggc ccttggcgtc
     8101 cttgatccac tcgaccagga acggctcctg gtggacgccc tggttggccc aggtcccgaa
     8161 ggcggaggcg agctgcaggg tggtgacgtc gctcggcccg agcgccagcg aggggacgtg
     8221 ggcgaggttc ggcttgtgct cagcggagtt gagggtggta tccaggccgg tacggtgagc
     8281 gagcgccgcg acccggtccg gccccgactg gccgttccgg gcgagctggt tgagcagcat
     8341 cgtgtaggcg gtgttgaccg attgggtggt cgcctgcagc agggagatct tgccgctgaa
     8401 gtgctcgccc gcgaagttgc ggatcgtctg gacgtcgtcg ggcttcggct tcccggtcgg
     8461 gatcagcaac ttgtccggcg cgaggagcgg ctggtccagg gtgaccccgt cgagcagggc
     8521 ctgctccagc acgaacggct tgaaggtcga accggcctgg cgcccggggg ggtcggtggc
     8581 gtagtcgaac tggtgggtgg cccactgctt ggcgctggcg ccagcgtaga tggcgcgcac
     8641 cgccccggtc ttggggtcga gcgcgaccag cgaggcgggc gggtccccct gctggttgag
     8701 gatgcccttc acggcggcct cggcctgggc ctgcatcttg aggtccaggg tggtgcgcac
     8761 gctgaagccg ccctgggcga ccgcggcggc gccgaactgg tcgaccaggt actggcgcac
     8821 cagatcgagg aagaacggcg ccttggagtt ggcgatgccg ctggcgtgaa ccgtccagtt
     8881 ctcggcaatc ggggcggcct cggccttcgc ggcggcgtcg gcgctgagcc agccgcgctg
     8941 ttgcatcacg ccgaggatga agttgcggcg ctccttggcg atccgcgcgt tcggcttgcg
     9001 ggtgtagacc tcgggggcgc ggatggctcc ggccagggtc gccgcctgcg ccggggtgag
     9061 cttctcgacc gacttgccga agaaggcgtg cgcggcggcg tccacgccgt aggcgccgtg
     9121 gccgaagtag accgcgttca ggtaggagcc gaggatctgg tccttggtgt agtggtgctc
     9181 cagcttgatg gcgatcacgg cttccttgac cttgcggaac atggtccgct cgttgccgac
     9241 cgccgggaag acgttcttca cgtactgctg ggtgatggtc gagccgccct cggcgacgcg
     9301 gcgctggacg acgttggacc aggcggcgcg cagcagcccc ttgtaggaga gggccccgtg
     9361 cttgtagaag tcgcggtcct cggcggcgag caccgcctcg cgcatcaccg gcgggatgtt
     9421 cttgaagtcg accacctggc ggttggcgat cggcgagatg gtgccgagcg gggccccgtt
     9481 ggcgtcgctg aaggagatgc tctgcgccgg gggcgcctgg ttggtgaccg gcagcttcgc
     9541 gaaggcgtag accacgccga tcacgacgcc gaagagaatc gcgatgactg gcacggtcag
     9601 ccatagcacc cagaagagga tgcgcttggt gcggggccgc tggggggcgg tgtggagccg
     9661 tgcggttcgt gagggcacgg tggtacgggt cgccatggcc aaggactcta ccccagggca
     9721 ggcgaaaacc tggaaccggg ccaacctggg gggatcaagc gggaaatttc agccggctgg
     9781 gcaggcgcgc ggacgggtca gccggcgtgg cggcggccga gcaggtagtg ctgggcgagg
     9841 tgattccagg agcagcccgt gcagacctcg accacgtagc aggcgaactc gtccatggtg
     9901 cgatgcaggt cggcgagggc ggcgctgggc cagacgcggc cggactcctg ccggagggcg
     9961 tcgccgaaca cgtaggtgac gtgggtgagg caggtctcgg cgcagaccgg gcagggggtg
    10021 cgggtcgatt cacccacgtt tcgggcggcg cgcagcagct cggggtgggc gtcgcagacg
    10081 tcgccgcggg tgaggccccc gaagcgcagc tcccgcagct tcgcccgttt ggcgagggtg
    10141 taatcgacca ggcgcatgga ggcagtgtag ggcgacaagg cagctatttg acagcctgat
    10201 cctggcgcat ctatcgtgtc gatacatcga gtcgatatat gtcgagagaa ccgcgagggg
    10261 gccccacccg tgctcgagct tgccgtcctt ggcctgctca aggagtcgcc catgtccgga
    10321 tacgagctga aaaagcggct taccgtgacc catggcgcct tctggcgagt cagcttcggc
    10381 tccctctacc cctgcctcaa gcggctgctc gccgacgggg cgctcgcggt ggaggagtcg
    10441 tcgcagttca cctcgcgcaa gaagcacgtg taccggttga ctgagcatgg cgagcggctc
    10501 ttccaggaga tgctcgagtc gaccgacgcc cacgacctcg agcaggaccg cttctccctg
    10561 cgactggcgt tcttccgcta cctgggtccg gagagccgcc tggaccagct cgaacgccgc
    10621 cggggctacc tccaggagcg gctcgccgac ctgcgcgcgt cgctgcgcac ggtccgcgac
    10681 cgcctggacg cctacaccat cgagctgatg gaccacgggc tcgacgcgca cgagcgtgag
    10741 atcgcctggc tcgaccgcct gatcgcggcc gagcggcact cgacctcggc cgcggccgcg
    10801 gcctcggcgc cgcagccccc gcccctgccc gcgcccccac cggaccagaa accggcaaca
    10861 acgccggcac gaacagaagg agtcaagacg taatggccaa ggtacgcgtc gcgatcgtcg
    10921 gcgtgggcaa ctgcgcctcg tcgctggtgc agggcgtcca gtactaccag gacgccctgg
    10981 agggggacga cgttcccggg ctgatgcacg tcgagctcgg tggttaccac gtccgggaca
    11041 tcgagttcgt cgccgctttc gacgtcgacg ccaagaaggt tgggctcgac ctcgccgaag
    11101 cggtgctcgc cagcgagaac aacactatcc acctggccga cgtcccgccc ctcggggtcg
    11161 aggtgcggcg cggcccgacc ctggacggcc tcggccgcta ctaccaggag atgatcaccg
    11221 agtccgagca ggcgccggtc gacgtggccg ccgcgctgcg ggacgcccgc gccgacgtgc
    11281 tggtctgcta cctgccggtc ggctccgagg aggccgcccg ctcctacgcc gcccaggcga
    11341 tcgaggccgg agtcgccttc gtgaactgcc tgccggcgtt catcgccacc gaccccgcgt
    11401 gggcggagcg cttccgcgcc gccgggttgc cgatcatcgg cgacgacatc aagtcgcagg
    11461 tgggcgccac catcgtgcac cgggtgctga gccgcctgat ggaggaccgc gggatcacca
    11521 tcgaccgcac ctaccagctc aacttcggcg gcaacatgga cttcatgaac atgctggagc
    11581 gcgaccggct gcagtcgaag aagatctcca agacccgggc ggtcacctcg cagctcgagc
    11641 acgacctcgg caagcgcaac gtccacgtcg ggcccagcga ccatgtcccg tggctggacg
    11701 accgcaagtg gtgctacatc cgcatcgagg gccgcgcctt cggggacgtc ccgctcaacc
    11761 tggagctcaa gctcgaggtc tgggactcgc ccaactcggc tggcgtggtg atcgacgcga
    11821 tccgctgcgc caagctcggc ctggaccgcg gtcttgccgg cccgctgctg ggcccggcct
    11881 cgtacttcat gaagtcgccg ccggtgcagt acaccgacga cgtcgcccgc caggccgtcg
    11941 aggacttcat cgccaacaag acctgaccca tgcgctgaac cggccgcctc gccgcgccgt
    12001 cgcccacaag gaacccgggc ggcggcgcgt ttcgcttgcg cgcagctgcg ctcccgcgcc
    12061 gcagaagacc tggtcgcagg gccgtctgga agccgcggaa agtgatcctc aaggcagggc
    12121 agggacggaa gctgccgaga taagggcgta tctgcacccg ccgcgtgccc tcgcatgggc
    12181 acagaaagga ctcgtggatg ccgagtacaa ccaaccgccg cccgctccgg cgccttctcc
    12241 tggtgccggt gctcggcgtc gcggtgctgt caagcgtcgc attcagcggg atcgcctacg
    12301 cggcgacccc gcaagcggcc acagcgccgg cgcagccgtc ggccgacaag ccggcgaccg
    12361 acagctccag cggcagctcg agcgctgaca acagctccag ctccgccccc tccaacccac
    12421 tggacatcgt cctcaacgtg ctgaagccgg tcctcgcgct ggtccagggc ctcccagacc
    12481 agctcctcgg ggccctgggc accagtggca gcagcagctc cgacaacagc accaccacca
    12541 ccacgagtgg cagcagcgcc ggcaccgcga agaccgaggt ggccatccca gaaggcaccc
    12601 aggtcatcgc cgcctgccag gagccgtcgg ccgacgccta cacggtccac gccgcgctcc
    12661 cgcaggccgt ctccggtctt caggaaggca cctcgatcca gctggactcg agcgcgctga
    12721 ccggcctcgc cggccaggcc gtgccgaacg tgccgatgtg cgccagcagc gacagcatga
    12781 tcagcaacag ctaggcagca tcacttcagt accccgggga ggccgtggtc gcgcgcccag
    12841 tcgtggagcg cggcgacggc ctcctcattt gtcatctggc cccggtccag gcgcagctcc
    12901 agcaggtgcg cgagcgcctg accgacgagg ggaccgggcg tgatcccgag gatctccatg
    12961 acctgccggc cgtcgagggg cggcttgatg ctcgccagct cctcctcggc ggccaggcgg
    13021 gcgatccgct cctccagctc gtcgcaggcg gcggcgagct gccgtgcttt gagggcattg
    13081 cgggtggtgc agtcggagcg gaccaggaag ttgagccggt cgaacagcgg gccggcgtcg
    13141 cgcacgtagc ggcgcaccgc cgagtcggtc cagccaagcc gataggtatg gaagcgtagg
    13201 tgcagttcga ccaggcggcg cacgtcctgg atgaagtcct tggcgtagcg cagctcggtc
    13261 aggcgccgct cggccatctt ggcgccgacc acctcgtgca ggtggaacga gacgccgtca
    13321 gagccgaccc gccgggtgcg cggcttgccg atgtcgtgca gcagcgcggc catgcgcagg
    13381 gtcaggtcgg gctcgtcctg gtcgaaggcg gtggcgcgct cgaccaccgc gatggtgtgg
    13441 gcgaggacgt ccttgtgccg gtggaccggg tcctgctcca gcgccagcgc cggcagctcc
    13501 ggcaggaagc gagcggccag gccgctgttg acggtgagcc agaggccgtt gccgggggcg
    13561 cggccgcaaa gcagcttgga gagctcgtcg cggatgcgct cccgcgagac gatgtcgagc
    13621 cgctcgaccc gctcgccgat ggccgcgagc gtctccctgg tcggctgcag ctccagctgc
    13681 gacacgaagc gggcggcgcg cagcatccgc agcgggtcgt cctcgaatga gccgaccggg
    13741 tcgccggggg tccgcaggca tcgggcggcc aggtcggcga ggcccccgaa ggggtcgacg
    13801 aactggccct gcggcaggtg tacggccatg gcgttgacgg tgaagtcgcg gcgtgccagg
    13861 tcctcctcga tggtcgttcc gaaggtgacc tcgggcttgc gggagtcgtc gcggtagatg
    13921 tcggagcgga aggtggtgac ctccagccgc aggtcgccct tgcgcacccc gacagtgccg
    13981 aagcggacgc cctgcagcca gacgccgtcg tgccagtggc cggcgcgcac cagcgccagg
    14041 atctcgtccg gggtggcgtc ggtggtgaag tcgacgtcgg gggaggggcg ctccagcagc
    14101 gcgtcccgga ccgtcccgcc gaccaggtag agctggtggc cgg
//
LOCUS       DASWDC010000241         5291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1397326, whole genome shotgun sequence.
ACCESSION   DASWDC010000241 DASWDC010000000
VERSION     DASWDC010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397326"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VF995_07930"
     CDS             <1..593
                     /locus_tag="VF995_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HEX9343526.1"
                     /translation="RVSPALPWLLHCPPHSLSTEAVALMLGRLRRDPDFLTQILGADP
                     RQAATLARRSQELLRDERLVFIRWCLVMVRFEQAIYRDPAADLTSVWWDLVERLQGLH
                     RPEGRQQPDWAAKIHLAHAPVYYQNYALGELMAAQLDAAVRAAAGGFIGRPEAGAFLT
                     ERVFAPGASFTWSGLIESATGRPLGVEAFLDDSTRG"
     gene            728..1057
                     /gene="fdxA"
                     /locus_tag="VF995_07935"
     CDS             728..1057
                     /gene="fdxA"
                     /locus_tag="VF995_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005293116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX9343527.1"
                     /translation="MTYTIAEPCIDVKDKSCIEECPVDCIYEGDRMLYIHPDECVDCG
                     ACEPVCPVEAIFYEDDVPAQWKEFTPANAAFFTEGDDPLGSPGGAAKVGPLVRDAAFI
                     ANYQMPE"
     gene            complement(1087..2271)
                     /locus_tag="VF995_07940"
     CDS             complement(1087..2271)
                     /locus_tag="VF995_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HEX9343528.1"
                     /translation="MVASDAAKTQWRELYHEVVETNLCTGCAACVMACPRDVLDYDHT
                     TSYHPFNVELSTAFDDCVHGERGCDICTRACPRFRAWEIDSDEALFGRPREADEVVGI
                     HRGIWLTRAADPAVLAAGQDGGLVSALLIWGLETDRIEGALTSRLSTTRLWDCEPFLA
                     TNQAEVLEAAGSRYTYAANPLAMKEAERRGLKRLALVGMSCQASINGTLAARRLNKYA
                     RRIELTIGLLCSKSFEYEGMKQVITEEYGIPLEDVAKVNIKGKFQLWRRSTGEQVDIP
                     LKRMQAATREGCRQCPDFAAQHADISTGGLGQRDGWTLTIVRTQRGEDWLNGAIQAGV
                     VEARPGEEDPNALALMAKLAIRSRERWPIAPGTLDGSGAPRLLPIAGVSAGGATSAAA
                     AS"
     gene            2394..2855
                     /locus_tag="VF995_07945"
     CDS             2394..2855
                     /locus_tag="VF995_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343529.1"
                     /translation="MAVRTRTTTTTIAITVALAVSALASMAFTVALHDTFTSGLVTFV
                     MFGSLAALSAGVLAWEVASRRVCPRCGTESGDPAACAACGYDLRARPRFACTEGHHVA
                     YEGGLCDCGRRLLPLRPVVIAGRLARTFWLAVAFFLAFLVLDALLSLHGNS"
     gene            complement(2954..3451)
                     /locus_tag="VF995_07950"
     CDS             complement(2954..3451)
                     /locus_tag="VF995_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343530.1"
                     /translation="MKRLGRHMGWLGWVVAGVLALSAGGVAWAATSSSPTTTTAPSAS
                     GSATKGEHPKLGHGLGRGLGGRLLHGEVTVQTKNGTQNVVLARGKVSAYTAGSSITIT
                     STDNTATTFAINSDTRYGFLNFSQPEASLKTGETALVVGAQSGSTNTAKRIVTVQNPP
                     QSSSS"
     gene            complement(3592..3834)
                     /locus_tag="VF995_07955"
     CDS             complement(3592..3834)
                     /locus_tag="VF995_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX9343531.1"
                     /translation="MFNALRDEVGKRLLTSVKVVATGCMEGCLAGPCLLVVPDNVWYG
                     GVTEADVPLLFDQHLAANGDGEPVEMLRLEPDDFCA"
     gene            4028..4456
                     /locus_tag="VF995_07960"
     CDS             4028..4456
                     /locus_tag="VF995_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9343532.1"
                     /translation="MDSQAGTLGREEQRELLKSAKVIAVVGLSHNPERPSNQVAWYLH
                     HQGYELYGVNPRLAGQDVSGIPVVASLAEVPKPIDIVDVFRRAEYTPDVAREAVAVGA
                     RALWLQLGIRNPETRRIAIAAGVDYVEDHCLKVEHLRLLG"
     gene            complement(4466..>5291)
                     /gene="folP"
                     /locus_tag="VF995_07965"
     CDS             complement(4466..>5291)
                     /gene="folP"
                     /locus_tag="VF995_07965"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9343533.1"
                     /translation="SREVAVAAIINRTPDSFYDRGATWQFGEALDAADRALEEGADWL
                     DVGGVKAAPGPEVPLAQELDRVLPLIEVLRARTGAVLSIDTFRAEVARRAIDVGADVV
                     NDPFGLRDPEMASVAAETGAGLVVMHSGGPPRTHIPQPVYADVVAAVAGFLTERTELA
                     RRRGVAAERLIIDPGFDFGKTTAHSLELVRRLRELTALGYPVLAAPSNKDFIGETLDL
                     PVDQRLEGTLAAVVACVLNGASIVRVHNVAATVRAVRLTESVLGWRPPAAPRRGLG"
BASE COUNT          711 a         1857 c         1902 g          821 t
ORIGIN      
        1 accgcgtcag ccccgccctg ccgtggctgc tgcactgccc gccgcacagc ctcagcaccg
       61 aggcggtcgc gctgatgctc ggccggctgc gccgcgaccc cgacttcctc acccagatcc
      121 tcggcgccga cccccgccag gccgccaccc tcgcgcgccg ctcccaggag ctgctgcgcg
      181 acgagcgcct ggtgttcatc cgctggtgcc tggtgatggt ccgcttcgag caggccatct
      241 accgcgaccc cgccgccgac ctcaccagcg tctggtggga cctggtcgaa cggctccagg
      301 gcctgcaccg ccccgagggc cgccagcagc ccgactgggc cgccaagatc cacctcgccc
      361 acgccccggt ctactaccag aactacgccc tcggcgagct gatggccgca caactcgacg
      421 ctgccgtccg cgcggccgcc ggcgggttca tcggccgacc cgaggcgggc gccttcctca
      481 ccgagcgggt cttcgcaccg ggcgcatcct tcacctggtc cgggctgatc gaatccgcca
      541 ccggccgccc cctcggcgtc gaggcatttc tggacgactc gacgcgcggg tgaggcggga
      601 ggagcggtag gattcgggcg gcgcccagtg gtggtcgtcc caggctgtcc ccgttggttg
      661 acggttccgg atggccggtg gtatccgtca aggcgaagaa cgtatccaca agggggtcaa
      721 cccgaagatg acctatacca tcgccgagcc gtgcatcgac gtgaaggaca agtcctgcat
      781 cgaggaatgt ccggtcgact gcatctacga aggcgaccgg atgctctaca tccacccgga
      841 tgaatgcgtc gactgcggcg cgtgcgagcc ggtctgcccg gtcgaggcga tcttctacga
      901 ggacgacgtt ccggcgcagt ggaaggagtt caccccggcg aacgcggcct tcttcaccga
      961 gggcgacgac cccctcggct ccccgggtgg tgcggccaag gtcggcccgc tggtgcggga
     1021 cgcggcgttc atcgcgaact accagatgcc cgaatagccc acccgaaggg cgggctgctc
     1081 gaaggctcaa ctggcggcgg ccgccgaggt ggcgccgcca gcgctcactc cggcgatcgg
     1141 cagcaggcgc ggtgcgccgc tgccgtcgag cgtgcccggc gcgatcggcc agcgctcccg
     1201 ggagcggatc gcgagcttcg ccatgagcgc gagcgcgttc gggtcctcct cgccggggcg
     1261 cgcctccacg acgccggcct ggatggcgcc gttgagccag tcctcgccgc gctgggtgcg
     1321 gacgatggtg agggtccagc cgtcgcgctg gccgaggccg ccggtggaga tgtcggcgtg
     1381 ctgggcggcg aagtcgggac actgcctgca gccttcgcgg gtggccgcct gcatgcgctt
     1441 gagggggatg tcgacctgct cgccggtgga gcggcgccac agctggaact tccccttgat
     1501 gttgaccttg gcgacgtcct ccagcgggat gccgtactcc tcggtgatga cctgcttcat
     1561 gccctcgtat tcgaacgact tggagcagag caggccgatg gtcagctcga tccgccgtgc
     1621 gtacttgttg agccggcggg ccgccagggt gccgttgatc gacgcctggc agctcatccc
     1681 gaccagggcg agccgcttga ggccgcgccg ctccgcctcc ttcatggcga gcgggttggc
     1741 cgcgtaggtg tagcgcgacc cggccgcctc cagcacctcc gcctggttgg tggccaggaa
     1801 cggctcgcag tcccacaggc gggtggtgga cagccgcgag gtgagggcgc cctcgatccg
     1861 gtcggtctcc aggccccaga tgagcagcgc ggagaccagc ccgccgtcct ggcccgctgc
     1921 cagcacggcg gggtcggcgg cgcgggtgag ccagatgccg cggtggatgc cgaccacctc
     1981 gtcggcctcc cgggggcggc cgaacagggc ctcgtcggag tcgatctccc aggcgcggaa
     2041 gcgggggcag gcgcgggtgc agatgtcgca gccccgctcg ccgtgcacgc agtcgtcgaa
     2101 ggcggtgctc agctcgacgt tgaaggggtg gtagctggtg gtgtggtcgt agtcgagcac
     2161 gtcgcgggga caggccatca cacaggccgc gcaacccgta cagaggttgg tctcgacgac
     2221 ctcgtggtag agctcgcgcc actgggtctt ggcggcatca ctcgcgacca tgcggcctcc
     2281 ggtcgatctt cggtcgattt tggtgagact atctttctac taggctacct ggacgggggt
     2341 atgaactcag cttgagcctc ttccgatcga gcgcgtccga gtcggggcgg ccggtggccg
     2401 tgcggacgcg gaccaccacg accacgatcg ccatcaccgt cgcgctggcg gtctcggcgc
     2461 tggccagcat ggcgttcacg gtcgcgcttc acgacacgtt cacgtcgggc ctggtgacct
     2521 tcgtgatgtt cggcagcctc gcggcgctca gcgcgggggt gctcgcctgg gaggtcgcct
     2581 cacgccgggt ctgtccgcgc tgcgggactg agagcggcga tcccgcggcc tgcgccgcct
     2641 gcggctacga cctgcgggcc cgcccgcgct tcgcctgcac cgaggggcac cacgtcgcct
     2701 acgagggcgg cctgtgcgac tgcggccgcc ggctgctgcc gctgcggccg gtggtgatcg
     2761 ccggacgcct cgcgcggacc ttctggctgg cggtcgcgtt cttcctcgcg ttcctggtgc
     2821 tggacgcgct gctgagcctc cacgggaaca gctgagccgc ctccgcggac acgaagggac
     2881 gccggcccct gggtgaggcc ggcgtcctga gagggcacgg ccgggcgccc tcgctccgta
     2941 ctggctatgt ccgctaggac gacgaggatt gcggtggatt ctgcaccgtg acgatccgct
     3001 tggcggtgtt ggtgctgccc gactgcgcgc cgaccaccag ggcggtctcg cccgtcttca
     3061 ggctggcctc cggctgggag aagttcagga agccgtagcg ggtgtcgctg ttgatggcga
     3121 aggtcgttgc ggtgttgtcg gtgctggtga tggtgatgct cgatccggcg gtgtaggcgc
     3181 tgaccttgcc gcgggcgagc accacgttct gggtgccgtt cttggtctgg accgtgacct
     3241 cgccatgcag cagccgcccg cccaggccgc ggcccaggcc atgcccgagc ttcggatgct
     3301 cgcccttggt ggcgcttccg ctggcggacg gggcggtggt ggtggtgggg gagctgctgg
     3361 tggccgccca ggcgaccccg ccagcggaca gggccaggac gcccgcgacc acccagccga
     3421 gccaccccat gtgcctgccg agccgtttca ttgactcacc tctgcgacgg ttgacggtgg
     3481 aggttccctt caagggcatg atccccgccc ttgatcagcg gaagcggcag cgattgttcg
     3541 ggcttcgtaa gagttcggga cgccgcccgg cggtcagacc ccttcagccc ttcaggcgca
     3601 gaagtcgtcg ggttccagcc gcagcatctc gaccggttcg ccgtcgccgt tggcggccag
     3661 atgctggtcg aacagcaacg gaacgtcggc ctcggtgacg ccgccatacc agacgttgtc
     3721 ggggacgacc agcaggcagg gcccagccag gcagccctcc atgcagccag tggcgaccac
     3781 cttgacgctg gtgagcaggc gcttgccgac ctcgtcacgc agcgcgttga acacctccgc
     3841 cgcaccccgg tccaggcacg accccttcgg gtggcccggc gggcgctggt tgaggcaaac
     3901 gaagacgtat ttttgtggct gggccaattg ctcggcgctc ctcggtgccc ggttcttctc
     3961 ggccaggcta ccggtggttg tcgatttcgg ggggtgcccg ttcgtcgctc ccggctacca
     4021 tggcgccatg gacagccaag cagggacgtt gggcagggag gagcagcgtg agctgctcaa
     4081 atcggcgaag gtgatcgccg tggtggggct gtcgcacaac cccgagcgac cctccaacca
     4141 ggtcgcctgg tacctgcacc atcagggcta cgagctgtac ggggtcaacc ccaggctcgc
     4201 cggccaggac gtgtccggca tcccggtggt cgccagcctc gccgaggtgc ccaagccgat
     4261 cgacattgtg gacgtgttcc gccgggccga gtacaccccg gacgtcgcca gggaggccgt
     4321 cgcggtcggc gcccgcgcgc tctggctcca gctcggcatc cgcaaccccg agacccgccg
     4381 gatcgccatc gccgccgggg tggactacgt ggaggaccac tgcctcaaag tcgagcacct
     4441 gcggctgctc ggctagccgg ccgcctcagc cgagcccccg gcgcggcgcg gcgggggggc
     4501 gccagccaag gaccgactcg gtgaggcgga ccgcgcggac ggtcgcggcg acgttgtgga
     4561 cccggacgat gctggcgccg ttgaggacgc aggcgaccac cgccgccagc gtcccctcca
     4621 ggcgctggtc gaccgggagg tcgagggtct cgccgatgaa gtccttgttg gagggagccg
     4681 ccagcaccgg gtagccgagc gcggtcagct cccgcaggcg tctgaccagc tccagggaat
     4741 gggcggtggt cttgccgaag tcgaagcccg ggtcgatgat caggcgctcg gcggcgacgc
     4801 cgcgccggcg ggccagctcg gtccgctccg tcaggaagcc ggccaccgcc gcgaccacgt
     4861 cggcgtagac cggctggggg atgtgggtgc ggggcgggcc gccgctgtgc atcaccacca
     4921 ggccggcgcc ggtctcggcg gcgacgctgg ccatctccgg gtcgcgcagc ccgaacgggt
     4981 cgttgaccac gtcggcgccg acgtcgatgg cccggcgtgc cacctccgcg cggaacgtgt
     5041 cgatcgacag caccgcgccg gtgcgggccc gtagcacctc gatcagcggc agcacccggt
     5101 caagctcctg cgccaggggg acctcggggc ccggggcggc cttgacgccg ccgacgtcga
     5161 gccagtcggc gccctcctcc agggcacggt cggcggcgtc cagcgcctcg ccgaactgcc
     5221 aggtcgcgcc ccggtcgtag aacgagtcgg gcgtccggtt gatgatcgcg gccaccgcga
     5281 cctcgcggct g
//
LOCUS       DASWDC010000242         4113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_564362, whole genome shotgun sequence.
ACCESSION   DASWDC010000242 DASWDC010000000
VERSION     DASWDC010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564362"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VF995_07970"
     CDS             complement(<1..798)
                     /locus_tag="VF995_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9343534.1"
                     /translation="MQSAAQSAAEIRAIAQSRVRLELLANLVRKDLKVKYKNSALGFL
                     WSLANPLLYLVVFGLVFGVFLKNGIPDFWVLFMSGLLVWNFFNLSTLSATGSVIGNAN
                     LVRKVRFPRVVLPLSSIGFAGVHFLLQLGVLLVVLVAFYRHAFGPQLVLLLPALLVVL
                     LFTGGMALLASSLNVRYRDVEHLLEIALIAWFWLTPIVYSVSLARSELLGRFHNLLWY
                     VYMGNPMTAVVTSMQRAIYRTTTVINTQSKQPYQVLASDGYRFYLKWL"
     gene            complement(938..2479)
                     /locus_tag="VF995_07975"
     CDS             complement(938..2479)
                     /locus_tag="VF995_07975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9343535.1"
                     /translation="MAIYNRFLPSMGGGERHSCMLAQVLAEEGHDVELIAHDDVGKEI
                     LGDHLGLDLTKVNLRIVPDKGDLQLAGLSAAYDLFVNASYMSRVKPRARHNLYLCYFP
                     TPFDHDLSRLRRRLARVLGPQVRRGKPDLVGWGDGWFPPEGGRRRTWAWTSGHGVLRL
                     PAGEGRRIAFDLGRPGAPEPAELVLRDERGAQLATLTADPKRFEHHEIPLLPNLGEHE
                     VSFGSDTFVPGEGDPRSLGVAVSRLHMAGAHRDLRRRLGERFPWLLRNPDDLSFLDHY
                     QRVLANSEYTRAWIQRYWKVDSDVLFPPIRVPDQPSAASKTRKILSVGRFFPPGMGHS
                     KKQLEQVRAFGQMIRKGSLDGWELHLAGGCRPAQRPYLEKVRRAAEGLPVQIHANAPR
                     ALVDELFATSSIFWTATGLGEDERTRPWVFEHFGITTAEAMANGCVPVVIDKAGQREI
                     VRHGVDGYRWSTLEELETYTRVLAADRALRERLAAAAATRAQEFSEPAFAARWAELEE
                     RLGLI"
     gene            complement(2542..3384)
                     /locus_tag="VF995_07980"
     CDS             complement(2542..3384)
                     /locus_tag="VF995_07980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9343536.1"
                     /translation="MTRVESDYYTHMSFDPEHTRQVLKHYVPMFTGHGPVLELGCGRG
                     EFLGLLKAAGVDAVGVDSDEGMVEAVTAAGQAAVLGDAAEYLHQEPAPGPYGGVFCAH
                     FVEHLTPDEVAHLLAGVRRVLAPGGRFVAVTPNPACYAVLSHDFWRDPTHVRFYDLPL
                     LEFFCRQAGLAVEGTGTNPLNHPGPPPRFLSADPVVHPGIGDLVDNAMGKLPEALAHS
                     VRRGREVDPHEAAWVYELAHLVKVLGDRLQETSEALRALRRAHDNLVWGLYEGNEIYV
                     VAKG"
     gene            complement(3414..>4113)
                     /locus_tag="VF995_07985"
     CDS             complement(3414..>4113)
                     /locus_tag="VF995_07985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9343537.1"
                     /translation="VLENHANLGYSRAVNLGMASTGASWILVANPDTRLEPGALARLV
                     ACGEADPRIGCVGPRLRNPDGTDYPTGRRFPSLLMGALHALLAPAWPDNPATRRYHLT
                     ELDRTHPVGVDWVSGACMLVRRSAFAAAGGFDESYFMYFEDMDLCLRLARGGWRVVFE
                     PRAAVHHTVGGSTRSAPYRKVWNHHRSALRFYCRRYARDPRILLAPLVAATLAARGAV
                     SLALARAKQPHRQS"
BASE COUNT          632 a         1340 c         1546 g          595 t
ORIGIN      
        1 gagccacttg aggtagaagc ggtagccgtc ggaggcgagc acctggtatg gctgtttgct
       61 ctgggtgttg atcacggtgg tggtgcggta gatcgcccgc tgcatcgagg tcaccaccgc
      121 cgtcatcggg ttacccatgt agacgtacca gagcaggttg tggaaccgcc cgagcagctc
      181 cgacctggcc aggctcaccg agtagacgat cggggtgagc cagaaccacg cgatcagggc
      241 gatctccagc aggtgctcga cgtcgcggta gcgcacgttc agcgacgagg cgagcaacgc
      301 catccccccg gtgaacagca acaccaccag cagcgcgggc agcagcagca cgagctgcgg
      361 cccgaacgcg tgccggtaga aggccaccag gacgaccagc agcacgccga gctgcagcag
      421 gaagtgcacc cccgcgaacc cgatcgagga cagcggcagc accacccgcg ggaagcggac
      481 cttgcggacc aggttggcgt tgccgatcac cgaacccgtg gcgctcaggg tcgagaggtt
      541 gaagaagttc cacaccagca gtccggacat gaacagcacc cagaaatccg ggatgccgtt
      601 cttcaggaac accccgaaca ccagcccgaa cacgaccagg tacagcagcg ggttcgccag
      661 cgaccacagg aagccgagcg cggagttctt gtacttgacc ttgaggtcct tgcggaccag
      721 gttggccagc agctccaggc gaacgcgcga ctgggcgatg gcccggatct cggccgcgct
      781 ctgtgccgcg ctctgcatga cggtctctct tccagctccg ggggtgtcca ggattcaggt
      841 tcagcttcag gttcgggacg attccaatgg gatggcgggc gcgcgtcccc ggtcgtggcg
      901 gggggttggc gcagtatacc cagcgcgtcg agccgggtca gatgaggccg aggcgctcct
      961 ccagttcggc ccagcgggcc gcgaaggcgg gctcggagaa ctcctgggcg cgggtggccg
     1021 cggcggcggc gaggcgttcg cggagggcgc ggtcggcggc gaggacgcgg gtgtaggtct
     1081 ccagttcttc cagggtcgac cagcggtagc cgtcgacgcc gtggcggacg atctcgcgct
     1141 ggccggcctt gtcgatcacc acggggacgc agccgttggc catggcttcg gcggtggtga
     1201 tgccgaagtg ctcgaagacc cagggccggg tgcgttcgtc ctcgccgagg ccggtggcgg
     1261 tccagaagat cgagctggtg gcgaacagct cgtcgaccag ggcgcggggc gcgttggcgt
     1321 ggatctggac gggaaggccc tcggccgcgc ggcggacctt ttcgaggtag ggccgctgcg
     1381 ccgggcggca gccgccagcg aggtgcagct cccagccgtc gaggctgccc ttgcggatca
     1441 tctggccgaa cgcccggacc tgttcgagct gcttcttgga gtggcccatg ccgggcggga
     1501 agaagcggcc gacggagagg atcttgcgcg tcttcgaggc ggcggagggc tggtcgggga
     1561 cccggatcgg cgggaacagc acgtcggagt cgactttcca gtaccgctgg atccaggccc
     1621 tggtgtactc ggagttggcg aggacccgct ggtagtggtc gaggaacgag aggtcgtcgg
     1681 ggttgcgcag cagccagggg aagcgttccc cgagcctgcg gcgcaggtcg cggtgggcgc
     1741 cggccatgtg gaggcggctg accgcgacgc cgaggctgcg cgggtcgccc tcgccgggga
     1801 cgaacgtgtc gctgccgaag gagacctcgt gctcgcccag gttggggagg agcgggatct
     1861 cgtggtgctc gaaccgtttc gggtcggcgg tcagggtggc gagctgggcg ccccgctcgt
     1921 cgcgcaggac cagctcggcg ggttccgggg cgccggggcg gccaaggtcg aaggcgatcc
     1981 ggcggccctc cccggcgggg agccggagga cgccgtggcc gctggtccac gcccaggtgc
     2041 gccgccggcc gccttctggc gggaaccagc cgtcgcccca gccgaccagg tcgggcttgc
     2101 cccggcggac ctgcgggccg agcacgcggg cgagccggcg ccgcagccgc gacaggtcgt
     2161 ggtcgaaggg ggtggggaag tagcagaggt agaggttgtg ccgggcccgt ggcttgacac
     2221 ggctcatgta ggaggcgttg acgaacaggt cgtaggcggc gctcaggccg gcgagctgca
     2281 ggtcgccctt gtccgggacg atccggaggt tgaccttcgt gaggtcgagg ccgaggtggt
     2341 cgccgaggat ctccttgccg acgtcgtcat gggcgatcag ttcgacgtcg tggccctcct
     2401 cggccaggac ctgggcgagc atgcaggagt gccgctctcc cccgcccatt gacggcagga
     2461 accggttgta gattgccacc ttcaaggttc gcgtccacct cgtcacagtc gcggttccgg
     2521 tcgccacggc gaccggaccg gtcagccctt ggcgacgaca tagatctcgt tgccttcgta
     2581 cagtccccac accaggttgt catgcgcgcg gcgcagcgcg cgcagcgcct ccgaggtctc
     2641 ctgcagccgg tcgccgagga ctttgaccag atgcgccagc tcatagaccc aggcggcctc
     2701 gtgtgggtcg acctcccggc ccctgcggac gctatgcgcg agcgcttctg gaagcttacc
     2761 cattgcgttg tcaacaaggt cgccaatccc tggatgaacg accggatcgg cgctcaggaa
     2821 tcgcggcggc ggcccggggt ggttcagcgg gttggtgccg gtgccctcca ccgccaggcc
     2881 ggcctggcgg cagaagaact ccagcagcgg caggtcgtag aagcggacat gggtcgggtc
     2941 gcgccagaag tcatggctga gcaccgcgta gcatgccggg ttcggcgtga ccgccacgaa
     3001 ccgcccgccc ggggcgagca cccggcggac gccggccagc aggtgggcga cctcgtcggg
     3061 ggtcaggtgc tccacgaaat gcgcgcagaa gaccccgccg taggggccgg gggcgggctc
     3121 ctggtggagg tattccgccg cgtcgccgag gacggccgcc tggccggcgg cggtcaccgc
     3181 ctcgaccatg ccctcgtcgg agtcgactcc gaccgcgtcg accccggcgg ctttcagcag
     3241 gccgaggaac tcgccgcggc cacagccgag ttccagcacc gggccgtgcc cggtgaacat
     3301 cggcacgtag tgcttgagga cctggcgcgt gtgctccggg tcgaaactca tgtgtgtgta
     3361 gtagtcactc tctacccggg tcaccggccg atgacctcct ctgcgactgt ccgctatgac
     3421 tgtcggtggg gctgcttggc gcgggcgagc gccagcgaca ccgcgccgcg ggcggcgagg
     3481 gtcgcggcga ccaggggcgc gagcaggata cgcgggtcgc gggcgtagcg cctgcagtag
     3541 aagcgcaacg cgctgcggtg gtggttccag accttgcggt agggggcgct gcgggtggag
     3601 ccgccaacag tgtggtgcac cgccgcgcgc ggctcgaaca ccacccgcca gccgccccgc
     3661 gcgagccgca ggcacagatc catgtcttcg aaatacatga aataggactc gtcgaagccg
     3721 ccggccgccg cgaacgccga gcggcgcacc agcatgcagg cgcccgacac ccagtcgacc
     3781 ccgaccgggt gggtccggtc cagctcggtc aggtggtagc gccgggtcgc cgggttgtcc
     3841 ggccaggctg gcgccagcag ggcgtgcagg gcacccatca gcagcgacgg gaagcgccgc
     3901 ccggtcgggt agtcagtgcc gtcggggttg cgcagcctgg gtccgacgca gccgatccgc
     3961 gggtcggcct cgccgcaggc gaccaggcgg gccagcgccc cgggctccag gcgcgtgtcg
     4021 gggttggcga ccaggatcca gctcgccccg gtgctcgcca taccgaggtt gacggcgcgc
     4081 gaatagccga ggttggcgtg gttctccagc acc
//
LOCUS       DASWDC010000243         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1535834, whole genome shotgun sequence.
ACCESSION   DASWDC010000243 DASWDC010000000
VERSION     DASWDC010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535834"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VF995_07990"
     CDS             complement(<1..268)
                     /locus_tag="VF995_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX9343538.1"
                     /translation="MTRPIRPFRVRAPATSANLGPGFDAFGLALDWWDTLRVSAPPGK
                     GEATTITVRGDGADAIPDGPDNLVRITMERVAADLGAELPPLRLQ"
     gene            complement(276..1364)
                     /gene="thrC"
                     /locus_tag="VF995_07995"
     CDS             complement(276..1364)
                     /gene="thrC"
                     /locus_tag="VF995_07995"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX9343539.1"
                     /translation="MNPITGQAAASPGVVERYRDQLPVSAATPVVSLLEGGTPLVPAP
                     VLAARTGCDVWLKIEGANPTGSFKDRGMTVAVSKALEEGSRAVLCASTGNTSASAAAY
                     AARAAITCAVLVPDGKIALGKLAQALVHGATVLAVDGSFDDCLTLARDLAEHYPVTLV
                     NSVNPYRIQGQKTVAFEIVEALGRAPDVHCIPVGNAGNITATWLGYREALEAGWATSR
                     PRMLGFQAAGAAPLVSGAPVAKPSTIATAIRIGRPASGEQALRAVAESSGALAAVTDR
                     QILAAYRLLAREEGVFVEMASAAAVAGLLQTAAKGDLRRGSAVVCTLTGNGLKDPDWA
                     VAGAPTPTRVPVDAAAAATALGLDPGSG"
     gene            complement(1372..2685)
                     /locus_tag="VF995_08000"
     CDS             complement(1372..2685)
                     /locus_tag="VF995_08000"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX9343540.1"
                     /translation="MAERGTVGIGMLGCGAVGTGVARLLADHAEPIRDRAGVPLALRR
                     IAVRDLTKKRDPVVDPTILGDEPAAVVEDPGVDVVVEVMGGIEPARSLILRALELGKP
                     VVTANKELLATRAGELFAAADRSGVDLRFEAAVAGAIPIIAPLKQSLAGDRLRRVLGI
                     VNGTTNYVLWRMASTGADYEDALAEATELGYAEPDPSADVEGFDAAAKCAILASLAFN
                     ARVVAGDVYREGITDVTAADIAAAARMGYTVKLLAIAEEVDGDVSARVHPAMIPDTHP
                     LASVTEAYNAVFVEGDAVGQLMFYGKGAGSLPTGAAVVGDLVEVARNRLQGARGVGCT
                     CLYERSVRSMDEVDTQYYLALDVADRPGVLAKVATVFGEHGVSIRSVLQRGKGDEAQL
                     VLVTHLAREGAMRATLAGLRQLDVVNDLQSVMRVEGEEADEVMIR"
     gene            complement(2794..3210)
                     /locus_tag="VF995_08005"
     CDS             complement(2794..3210)
                     /locus_tag="VF995_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9343541.1"
                     /translation="MTNARILVVDDDRVIQQLLKVNLELEGYVVEVAEDGEDALFQVA
                     MFRPDLILLDIMMPRLDGWEVSRRLKEDPRTASLPIVLLSARAQETDVQRGDELGVAA
                     YVTKPFDPIQLLDLVDGLLRTYGFGGDAYGGSWAPA"
     gene            3363..3438
                     /locus_tag="VF995_08010"
     tRNA            3363..3438
                     /locus_tag="VF995_08010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3396..3398,aa:Arg,seq:ccg)
BASE COUNT          499 a         1302 c         1291 g          505 t
ORIGIN      
        1 gctgcagccg cagcgggggc agctcggcgc cgaggtcggc ggccacccgc tccatggtga
       61 tgcggaccag gttgtcgggg ccgtcgggga tggcgtcggc gccgtcgccg cgcaccgtga
      121 tggtggtggc ctcccccttc cccggcggcg cgctgacccg cagggtgtcc caccagtcga
      181 gcgccaggcc gaaggcgtcg aaccctggcc cgaggttggc gctggtggcc ggggcccgga
      241 cgcggaaggg gcggatcggt ctggtcatga tctgctcatc cggagcccgg gtcgaggccg
      301 agggcggtcg cggcggcggc cgcgtcgact ggcacgcggg tcggggtggg cgcgccggcc
      361 accgcccagt cggggtcctt gagcccgttg ccggtgaggg tgcagacgac cgcgctgccc
      421 cgtcgcaggt cgcccttggc ggcggtctgg agcaggccgg cgacggcggc ggccgaggcc
      481 atctcgacga acacgccctc ctcccgggcg agcaggcggt aggcggccag gatctgccgg
      541 tcggtgacgg ccgcgagcgc cccggaggac tctgccaccg cccgcagggc ctgctcgccg
      601 gaggcgggcc ggccgatgcg gatcgcggtc gcgatggtcg agggtttggc caccggcgcg
      661 ccgctcacca gcggcgccgc gccggccgcc tggaagccga gcatgcgcgg ccgcgaggtc
      721 gcccagccgg cctccagcgc ctcgcggtag ccgagccagg tggcggtgat gttgccggcg
      781 ttgccgacgg ggatgcagtg cacatcgggc gcgcggccga gcgcctcgac gatctcgaag
      841 gcgaccgtct tctggccctg gatgcggtag gggttgaccg agttgaccag ggtgaccggg
      901 tagtgctcgg cgaggtcgcg ggcgagggtc aggcagtcgt cgaaggaccc gtccaccgcc
      961 agcacggtcg cgccgtggac cagcgcctgg gccagcttgc cgagggcgat cttgccgtcg
     1021 gggaccagta ccgcgcaggt gatcgccgcc ctggccgcgt aggcggcggc ggacgcggag
     1081 gtgttgccgg tggaggcgca cagcaccgcc cgcgagccct cctccagcgc cttggagact
     1141 gccacggtca tgccgcggtc cttgaacgac ccggtggggt tcgcgccctc gatcttgagc
     1201 cagacatcgc agccggtccg ggctgccagc accggcgccg gcaccagcgg cgtgccccct
     1261 tccagcaagc tgaccaccgg cgtggccgcc gagaccggca gctggtcgcg gtagcgctcc
     1321 acgacccccg gcgaggccgc cgcctggccc gtgatcgggt tcatctgcgc atcacctgat
     1381 catcacctcg tcagcctcct ccccctcgac ccgcatcacg ctctgcaggt cgttgaccac
     1441 gtcgagctgg cgcagaccgg cgagggtcgc ccgcatcgcg ccctccctgg ccaggtgggt
     1501 gacgagcacg agctgcgcct cgtcgccctt gccgcgctgc aggaccgagc ggatcgacac
     1561 gccgtgctcg ccgaagacgg tggcgacctt cgccagcacg ccgggccggt cggccacgtc
     1621 gagggcaagg taatactgag tgtcgacctc gtccatgctg cgcaccgaac gctcgtacaa
     1681 acaggtacag ccaacgccgc gggcgccttg gaggcggttg cgggccacct cgaccaggtc
     1741 gccgacgacc gcggcgccgg tcggcagcga gccggcgccc ttgccgtaga acatcagctg
     1801 gccgaccgcg tcaccctcca cgaagacggc gttgtaggcc tcggtgaccg aggcgagcgg
     1861 gtgggtgtcg gggatcatcg ccgggtgcac ccgggcgctg acgtcgccgt cgacctcctc
     1921 ggcgatggcc agcagtttga cggtgtagcc catgcgggcg gcggcggcga tgtcggcggc
     1981 ggtcacgtcg gtgatgccct cgcggtagac gtcgccggcg accacccggg cgttgaacgc
     2041 caggctggcg aggatggcgc acttggcggc ggcgtcgaac ccctccacgt cggcgctcgg
     2101 gtcgggctcg gcgtagccaa gctcggtcgc ctcggccagg gcgtcctcgt agtcggcgcc
     2161 ggtggaggcc atgcgccaca gcacgtagtt ggtggtgcca ttgacgatgc cgagcacccg
     2221 gcgcaggcgg tccccggcca gcgactgctt gagcggcgcg atgatcggga ttgccccggc
     2281 cacggccgcc tcgaagcgca gatccacgcc gctccggtcg gcggcggcga acagctcgcc
     2341 ggcccgggtg gcgagcagct ccttgttggc ggtcaccacc ggcttgccca gctccagagc
     2401 ccgcaggatc agcgagcggg ccggctcgat cccgcccatc acctcgacca ccacgtccac
     2461 gccggggtcc tcgaccaccg ccgccggctc gtcgccgagg atggtggggt cgaccaccgg
     2521 gtcccgcttc ttggtcaggt cccgcacggc gatccgccgc agcgccagcg gcaccccggc
     2581 gcggtcacgg atcggctcgg cgtggtccgc gagcaacctg gccacgccgg tgcccaccgc
     2641 cccacacccg agcatgccga tcccgaccgt gccgcgctct gccacgtcgc tcctccatgc
     2701 gcctctgggt ttgccgctgg actgagaatc catcttgccg gaccaggcca cggcttgctt
     2761 gggttatcca cagggccagg gggatcaggg ggatcaggcc ggcgcccagg agcctccgta
     2821 ggcgtccccg ccgaagccgt aggtgcgcag cagcccgtcg accaggtcga gcagctggat
     2881 cgggtcgaac ggcttggtca cgtaggccgc gacccccagc tcgtcgccgc gctgcacgtc
     2941 ggtctcctgc gcccgcgccg acagcagcac gatcggcagg ctcgcggtgc gcgggtcctc
     3001 tttgagcctg cgggagacct cccagccgtc cagccgcggc atcatgatgt cgagcaggat
     3061 caggtccggc cggaacatcg ccacctggaa cagcgcgtcc tcgccgtcct cggccacctc
     3121 gacgacgtaa ccctcaagct caaggttcac cttgagcaat tgctgaatca cgcggtcgtc
     3181 gtcgaccacc agaatgcggg cattggtcat gaagcgaaac cagggtccag aatggagagc
     3241 gcttggtcgc taccgcctcg cgcagcataa cataggcggc ctgacctgca ttttcataga
     3301 cgggaagcca actggccgcc ggatggtacc atacgccgcg cacccgattg gaccctcatc
     3361 gggcccacgt agctcagcgg atagagcact gccctccgga ggcaggtgtc gcaggttcga
     3421 gtcctgccgt gggcaccacc caaaccccca cgtcagccgc catgtcggca ctcgcttcat
     3481 cctgcgtcga gcccgtgttg ccgctgccgg gcagcaaacg ggcagcagtc ggcagcacga
     3541 tggccagccg ctcctcgacc gcagcggtga tgcgcgcctc catcgccggg gtggtgt
//
LOCUS       DASWDC010000244         6103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1066206, whole genome shotgun sequence.
ACCESSION   DASWDC010000244 DASWDC010000000
VERSION     DASWDC010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066206"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VF995_08015"
     CDS             <1..296
                     /locus_tag="VF995_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9343542.1"
                     /translation="HTNCQEAFFVLEGDVEFVLGDGDASPVRHGPGTFVLVPGGVPHT
                     FGNPGPGPARVLVLHAPAMDQYFRELRALWSGDIPPDRDTELDLMRRHGMEPG"
     gene            complement(332..1105)
                     /locus_tag="VF995_08020"
     CDS             complement(332..1105)
                     /locus_tag="VF995_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005485050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9343543.1"
                     /translation="MPILVGTSGWQYRDWRGRFYPQKLPQARWLEYFASQFATVESNN
                     AFYRLPTPDTFAAWAKRTPDDFVMAVKASRYLTHVKRLHDPEEPVRRLLDHAEHLGAT
                     LGPVLLQLPPNLRAEAELLDRCLRAFPKHVRVAVEPRHDSWWSDEIRALLDAHSAALC
                     LADRGNRISPLWRTAPWGYLRFHHGAASPPPSYGDRALATWASRLHDLFGPEADVYVY
                     FNNDQGAAAVRDAARFARAAEHAGLHPTSTPPPSAIRVE"
     gene            complement(1180..1710)
                     /locus_tag="VF995_08025"
     CDS             complement(1180..1710)
                     /locus_tag="VF995_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343544.1"
                     /translation="MAVKVVHVSDISGKEADEQALGKLIIHEHPEYSDLPVTLEVLPE
                     EVESLEGASRYVTVEWLHPGSRKGKRMTVGIEDFNLLAGPGVMESAVQEALIAKHRTR
                     AKSTGTTTATGRAARGKVNYATLEHAGEPHRGRITEAEKELVRTHLDEINERLRNTDL
                     REIDPNDPTMRDRYSL"
     gene            1980..2969
                     /locus_tag="VF995_08030"
     CDS             1980..2969
                     /locus_tag="VF995_08030"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX9343545.1"
                     /translation="MVAAFFAVTLFARWRQVTAVKWHLGGGYFVVACLLLGASYALVA
                     ALWGLALRAVAAVRPHPVAGARIWFVSNLARYVPGNVWSFVGAAELARREGVRRRDTL
                     AVMALTQGLSVGVALLVGLPVLLAERSRLGRPALVGTVLLLAAVVLALALWRPLWRRL
                     AVPLLARRYPGLLPSEGGEGGGRVPWPPPLTMVWLGAGYAFYWAVTGLAFAALYRSLF
                     PLAGGDVPLVVAAYAAAYAVGFLSLLTPAGLGVREGVLVVALAPLLPAGPALVVALLS
                     RLWMMLFELLGVAAAWLLERAGQRRSSRLDPDPAPAPAPDPDPAPGNGRRRSS"
     gene            complement(2873..3886)
                     /locus_tag="VF995_08035"
     CDS             complement(2873..3886)
                     /locus_tag="VF995_08035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9343546.1"
                     /translation="MRMDLHEEHDGRLVSVVVPAHNEAATVATFVERTAKAFAELGRP
                     WELVYVDDGSTDGTAAAIRRAATREPRVHLVSQRRNLGLTEALNRGFRAAAGDLIVFL
                     PADLESDPAVDIPLLLGKLDEGFDVVSGWRQGRRDGKRLASRLANVTCRLLFGLDVHD
                     MNWIKAFRREVTDGFAMRSSWHRYMLVLALDEGWRIGEVKVPYQPRQSGRSKFGFSRL
                     PVSFIDVVTLKFLLTFQRKPMLFFGAIGSAMIGAGVLLWAYLAWRYLSSHTQYRPLML
                     VAGIAVLAGLLVVLVGFLAELVVNVSERVDQLERRLPLPGAGSGSGAGAGAGSGSSRD
                     ERR"
     gene            complement(4154..5290)
                     /locus_tag="VF995_08040"
     CDS             complement(4154..5290)
                     /locus_tag="VF995_08040"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX9343547.1"
                     /translation="MAELRVPLVDLRAGHRVLRDELQAAIDRVIDSAAFIQGPEVAAF
                     ERAFAAFCGTQRAVGVASGTAALHLALLALGVMPGDEVVTVAHTFTATAEAIAQAGAI
                     PRFVDVDKATGGMDPDALAASLDETEAAAVLPVHLYGLPADMPAITAAAHAAGIHVVE
                     DAAQAHGASVLQVEGAAEKRAGALGTLACFSFYPGKNLGAFGDAGAVTTDDEERADQV
                     ARLRDHGRSSKYEHAEPGYGERLDTLQAAILLVKLAHLEAANAARARGAARYGEALAG
                     VGDLVLPAATPGRQSAWHLYVVRSAQRDGLLAHCRAAGIGAQVHYPIPLHLQPAWRHL
                     GYARGALPATEAWAAECLSLPLYPELTEEQQDHVIATVRGFFGG"
     gene            5653..>6103
                     /locus_tag="VF995_08045"
     CDS             5653..>6103
                     /locus_tag="VF995_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343548.1"
                     /translation="MLARRKWYLLVALTLTTSAALLLTRATPPLYRSEATLLLAPRAS
                     AAGMPLTPLTPLTPQVIASLADVASDRATALAVLRRYGAEFPLTTTALQRRISATAVD
                     GTLLLRVTVSDSDPAQAQQLADAVATTFAELLPRLQGPAVRASLISPA"
BASE COUNT          806 a         2068 c         2270 g          959 t
ORIGIN      
        1 ggcacacgaa ttgccaggag gcgtttttcg tgctggaggg cgacgtcgaa ttcgtcctcg
       61 gcgacggcga cgccagcccg gtgcggcacg gcccgggaac cttcgtgctt gtccccggcg
      121 gtgtgccgca caccttcgga aatcccggac cgggcccggc gcgggtcctg gtgctccacg
      181 cccccgcgat ggaccagtac ttccgcgagc tgcgggcgct gtggtccggc gacatcccgc
      241 ccgaccgcga caccgaactg gacctgatgc gtcgccacgg catggaaccc gggtaaccga
      301 caagtgggaa cgggaggtgg agcaggagga gtcactcgac ccggatcgcg ctcggcggcg
      361 gggtgcttgt cgggtggagg ccggcgtgct cggcggcgcg ggcgaaccgg gccgcgtcgc
      421 ggacggcggc ggcgccctgg tcgttgttga agtagacgta cacgtcggcc tccgggccga
      481 agaggtcgtg gaggcggctg gcccaggtgg cgagcgcccg gtcgccgtag gacggcggcg
      541 ggctggcggc gccgtggtgg aagcggaggt agccccaggg ggcggtgcgc cacagcgggg
      601 agatccggtt gccccggtcg gcgaggcaca gcgcggcgct gtgggcgtcg aggagggcgc
      661 ggatctcgtc gctccaccag ctgtcgtggc gcggctcgac cgccacccgg acgtgcttgg
      721 ggaaggcgcg cagacaacgg tcgagcagct cggcctcggc ccgcagattc ggcgggagct
      781 gcagcaggac cgggccgagg gtggcgccca gatgctcggc gtggtcgagc aggcgccgca
      841 ccggctcctc gggatcgtga aggcgtttca catgggtgag ataccggctg gctttcaccg
      901 ccatgacgaa gtcgtcaggg gtgcgctttg cccatgcggc gaacgtatcc ggcgtgggga
      961 gccggtagaa ggcgttgttg gactccacgg tggcgaactg gctcgcgaag tactcaagcc
     1021 atcgcgcctg cggcagcttc tgggggtaga aacggccacg ccagtcgcga tactgccagc
     1081 ctgacgtccc aaccaagatg ggcatgttga cccctgccca cggcagtgag cagcgttctc
     1141 tcagcgtatt ttaaggttca gctgaaacct atttggcggt tagagactgt agcggtcccg
     1201 catggtcggg tcgtttgggt cgatctcgcg caggtcggtg ttgcggagcc gctcgttgat
     1261 ctcatcgagg tgggtgcgga ccagttcctt ctcggcttcg gtgatgcgtc cgcggtgggg
     1321 ttcgccggcg tgttcgaggg tggcgtagtt gaccttgccg cgggcggcgc ggccggtggc
     1381 ggtagtggtg ccggtgctct tggcgcgggt gcggtgcttg gcgatcagcg cctcctgcac
     1441 cgcggattcc atgacaccgg gcccggccag caggttgaag tcctcgatgc cgacggtcat
     1501 gcgcttgccc ttgcgggagc cggggtggag ccattcgacg gtgacgtagc gcgaggcgcc
     1561 ttcgagggac tcgacttcct cggggaggac ctcaagggtg accggcaggt ccgaatattc
     1621 agggtgctca tggatgatga gcttcccgag cgcctgctcg tcagcctcct tgcctgaaat
     1681 gtccgagaca tggacgacct tcactgccat cagggtagat tcctccctca atccaaaagg
     1741 gatcgctcgt tggcttgtgg ctttacgcta atcgaaaacg tgtcggtaag gcaacgtcag
     1801 ctgcacaacg gggaccacgc tgcgaacaga tagtatcgcg agacacctcg gttaggtcgt
     1861 gggaaggcgg gatcgtgccg gcggaatccg aaggaccgcg ttcgcgagtg cgttcgcaag
     1921 tgcgttcgca agtgcgttcg caagtgcgtt cggcttggct caagtgggtc gccgccgcgg
     1981 tggttgccgc cttctttgcc gtgacgcttt tcgcgcgctg gcggcaggtc acagcggtga
     2041 aatggcacct tggagggggt tatttcgtgg tggcctgcct cctgctcggc gcctcctacg
     2101 cgctggtggc cgccctgtgg gggctcgccc tgcgggccgt cgccgccgtc aggccccacc
     2161 cggttgccgg cgcccggatc tggttcgtgt ccaacctcgc ccgctatgta ccgggaaacg
     2221 tctggagctt cgttggcgcc gccgagctgg cccggcgtga gggtgtgcgc cgccgcgaca
     2281 ccctggcggt gatggcgctg acccagggcc tgtcggtggg ggtggccctc ctggtcgggc
     2341 tgccggtcct gctggcggaa cgctcgcgcc tgggacgccc ggcgctggtc ggcacggtgc
     2401 tgctgctcgc cgccgtggtg ctcgccctgg cgctgtggcg gccgctctgg cggcggctgg
     2461 ccgtgccgct gctggcacgg cgctacccgg ggctgctgcc gtccgaaggg ggagagggcg
     2521 gggggcgggt gccgtggccg ccgcccctca ccatggtctg gctgggcgct ggatatgcct
     2581 tctactgggc ggtcaccggg ctggcgttcg cggcgctgta ccggagcctg ttcccgctgg
     2641 cgggcgggga cgtgccgctg gtggtggcgg cctacgcggc ggcctacgcg gtcgggttcc
     2701 tgtcgttgct gaccccggcg ggcctcggcg tgcgcgaggg ggtcctggtg gtcgcgctgg
     2761 cgcccctgct gccggccggc ccggcgctgg tggtggcgct gctgtcacgg ctgtggatga
     2821 tgctgttcga gctgctcggc gtcgccgccg cctggctgct ggagcgggcc ggtcagcgcc
     2881 gctcgtcgcg gctggacccg gacccggccc cggccccggc cccggaccct gacccggcgc
     2941 cgggcaacgg caggcggcgc tccagctgat cgacccgttc tgagacgttc accaccagct
     3001 cggcgaggaa gccgaccagg accaccagca gccccgccag cacagcgatc cccgcgacca
     3061 gcatcagcgg ccggtactgg gtatggctgg agaggtagcg ccaggccagg taggcccaca
     3121 agagcacccc ggcgccgatc atcgcggagc cgatcgcgcc gaagaacagc atcggcttgc
     3181 gctggaaggt gagcaggaac ttgagggtga ccacgtcgat gaacgacacc ggcaggcgcg
     3241 agaagccgaa cttcgagcgc ccgctctggc gcggctgata ggggaccttg acctcgccga
     3301 tccgccagcc ctcgtcgagc gccagcacca gcatgtagcg gtgccaggag gagcgcatcg
     3361 cgaatccgtc ggtgacctcg cggcggaacg ccttgatcca gttcatgtcg tggacgtcca
     3421 ggccgaacag caggcggcag gtgacgttgg cgaggcggct ggccagccgc ttgccgtcgc
     3481 ggcggccctg gcgccagccg ctgaccacgt cgaacccctc gtccagcttg ccgagcagca
     3541 gcgggatgtc caccgcgggg tcggactcca ggtcggcggg caggaacacg atcagatcgc
     3601 cggcggcagc cctgaagccg cggttgagcg cctcggtcag gccgaggttg cggcgctggc
     3661 tgaccaggtg gacgcgcggc tcacgggtcg cggcccggcg gatcgcggcg gcggtgccgt
     3721 cggtggagcc gtcgtcgacg tagaccagct cccagggccg gcccagctcc gcgaacgcct
     3781 tggcggtccg ctccacgaag gtggccaccg tggctgcttc gttgtgggcg gggacgacca
     3841 ccgacaccag gcgcccgtca tgctcctcat gaaggtccat gcgcatgggc ccatcatcgc
     3901 aagcgggggc ggcgggcggc cactcggcgt gcatcggact tcagggaatt gtcacttggg
     3961 agatccgcgg aggccgccga tacagagggt gaggcggcag ggacggacga atcgccgcct
     4021 cggagacgct cggctggctg gtcgccggtc ggccaagtcc agtcctggtg gcggcccacg
     4081 tgaggagagg tgcggatggt gggcccaccg gcgccgaccc tgaggggcgc cggcccgccg
     4141 ggcgatcaac cattcagccg ccaaagaacc cgcggacggt cgcgatcaca tggtcctgct
     4201 gctcctcggt cagctcggga tacagcggca gcgacaggca ctcggcagcc cacgcctcgg
     4261 tggccggcag ggcgccgcgc gcgtagccaa ggtggcgcca ggccggctgc aggtgcagcg
     4321 ggatcgggta gtgcacctgc gcgccgatcc cggcggcgcg acagtgcgcc agcagcccgt
     4381 cgcgctgggc gctgcggacc acatacaggt gccaggcgct ctgccgccct ggcgtggccg
     4441 cgggcaggac caggtcgccg acccccgcga gcgcctcgcc gtagcgggcg gcgccgcgtg
     4501 ccctggcggc gttggccgcc tccaggtggg cgagcttgac cagcaggatc gcggcctgca
     4561 gggtgtccag ccgctcgcca tagcccggtt cggcgtgctc gtacttggag ctgcgcccgt
     4621 ggtcccgcag ccgcgccacc tgatcggccc gctcctcgtc gtcggtggtc accgcccccg
     4681 cgtcgccgaa ggcgccgagg ttcttgcccg ggtagaagct gaagcaggcg agcgtgccga
     4741 gcgccccggc ccgcttctcc gccgcaccct ccacctgaag gacactggcg ccgtgtgcct
     4801 gcgccgcgtc ttccaccaca tggatcccgg cggcatgggc cgcggccgtg atcgcgggca
     4861 tgtcggcggg cagcccgtag aggtggaccg gcaggaccgc ggctgcctcg gtctcgtcca
     4921 gcgaggcggc gagggcgtcg gggtccatgc cgccggtggc cttgtcgacg tcgacgaagc
     4981 gggggatcgc gccggcctgg gcgatcgcct ccgcggtggc ggtgaaggtg tgcgccacgg
     5041 tgacgacctc gtcgcccggc atgaccccga gggccagcag ggcgaggtgg agggcggccg
     5101 tgcccgaggc cacgccgacc gcccgctggg tgccgcagaa cgccgcgaac gcccgctcga
     5161 aggcggccac ttcggggccc tggatgaagg cggccgaatc gatcacgcgg tcgatcgccg
     5221 cctgcaattc gtcgcgcagg acgcggtggc ccgcccgcag gtcgaccagg ggcacgcgca
     5281 actcggccat aggtgtgggt cctcgtctct ggtggagggt gtggggatcc tgggcttctg
     5341 gcatggtgca cccggccagt caggtggcgc gagtgggcca cctcgggcat ggcccagcgg
     5401 ggctacgatg ggccatctag gttctggtcg agggtttcga cagcttcaat gcttgcgtcg
     5461 cgttcgagac tcacttaggc tgcggaagcc gtcggcgggg ctcgcgcccg ttcctcgcgc
     5521 gaggcgcgag cacgccgcgg cgcctcctca ccaatcctgt cgttcccgct gttcccgtat
     5581 tcccgtattc ccgtattccc gtattccgct ctttccgctg ggggacccat ggcatgttct
     5641 cgcgcaggtc gagtgcttgc caggcgtaag tggtacctgc tcgtcgccct caccctgacg
     5701 acctcggcgg cgctgctgct gacccgggcg acaccgccgc tctaccgctc cgaggccacc
     5761 ctgctgctcg caccgcgggc cagcgcggcg ggcatgccgc tcaccccact caccccgctc
     5821 accccgcagg tcatcgcctc gctggccgac gtggccagcg accgcgccac cgccctggcg
     5881 gtgctgcgcc gctacggcgc cgagttcccg ctcaccacca ccgcgctgca gcgcaggatc
     5941 agcgccaccg ccgtcgacgg gaccctgctg ctgcgggtca ccgtctcgga ctccgacccg
     6001 gcacaagccc agcagctcgc cgacgcggtc gcgaccacgt tcgccgaact gctcccgcgg
     6061 ctgcagggcc cggccgtgcg cgccagcctg atcagtccgg cga
//
LOCUS       DASWDC010000245         6516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1397670, whole genome shotgun sequence.
ACCESSION   DASWDC010000245 DASWDC010000000
VERSION     DASWDC010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397670"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..2324
                     /locus_tag="VF995_08050"
     CDS             189..2324
                     /locus_tag="VF995_08050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9343549.1"
                     /translation="MTTTIPKPTTWGVADPAGGSRRAGLLRRLVRGRESDQPWVRPAL
                     LALLAATALLYLWSLGASGWANSFYAAAVQAGSKSWKAFFFGSSDASNFITVDKTPAS
                     LWIMDISARIFGVNSWSLLVPQALEGVAAVGLLYAAVRRWFSAGAALLAGAVMALTPV
                     AVLMFRFNNPDALLTLLLVAGGYAMVRAVEDGRTRWLVLAASCVGFGFLTKMLQALLV
                     VPAFALVYLVAAPTPLRRRIGQLLAAGVAMVVSAGWWVAAVALIPAANRPYIGGSQHN
                     SILELTFGYNGFGRLTGNETGSVGGGGLGGTGGRWGTPGLFRLFSEMGGEITWLLPAA
                     VVLLGAGLWLTRRMPRPDRTRAALLLWGGWLVVTGLTFSYAAGIIHPYYTVALAPAIG
                     AVVGIGAAFFWRYRSYLVARAVLAASLAVTGWWSYVLLERASGWQPWLAPLVLLGSLA
                     VAAVLLALPHLRLARRAALAVAAAGLIVALAAPAAYSMATAAQPHSGAIPSVGPASAG
                     GLGFGRPGGGNFGTNRQFGGTQGQGQAPGGFAFGTPGQGQGNGTTNGQGQASPFGGQG
                     NGNGNGNGNGGFGGRGGVGSLLNGSNPSSQLVALLKQGASGYTWTAAAVGSNSAAGVQ
                     LATGEPVMAIGGFNGTDPTPTLAQFQQYVRDGKIHYFIASGGGMTGGQGGSSTSSQIT
                     AWVEQNFKSTTAGGTTVYDLSTASSTNTA"
     gene            complement(2390..3205)
                     /locus_tag="VF995_08055"
                     /pseudo
     CDS             complement(2390..3205)
                     /locus_tag="VF995_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216234.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
     gene            3313..3462
                     /locus_tag="VF995_08060"
                     /pseudo
     CDS             3313..3462
                     /locus_tag="VF995_08060"
                     /inference="COORDINATES: protein motif:HMM:NF036707.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3576..4010
                     /locus_tag="VF995_08065"
     CDS             3576..4010
                     /locus_tag="VF995_08065"
                     /inference="COORDINATES: protein motif:HMM:NF036707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343550.1"
                     /translation="MVVEEFTGLPAEKLSIQFRDPSEFFDPGAYAAARASGAVSGAVL
                     GRVGLSHTPDREPSGAILGSRLLHIVRDTPWGAALRSHFFLGQDLPAQGLSPAEITTA
                     FSDEFGQALLQHAYNEFTFLSRFLPSLYIAENRDTKRPEVPW"
     gene            complement(4018..4803)
                     /locus_tag="VF995_08070"
     CDS             complement(4018..4803)
                     /locus_tag="VF995_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014588399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9343551.1"
                     /translation="MGTDRSNQERQSRAAGVQVIARAAELLRELQASPGGLAQAELAA
                     RVGLARSTVHRILTALEAEGLVMSRTRRGRYRLGPEFVRLAEGARHGLVAEIRPFLDK
                     LSRELDETVDLSIRDGDHVTFLDQVEAPQRLRAVSAVGASFPLHCTANGKAVLATLPP
                     DELAALLPPALPAFTVHTVTDPKRLLAELAQVRASGVAFDREEHTEGICAVGTSVLPS
                     SGAALAISVPMPALRFHGREQRLAETLLVTRRAVEDAFGAATA"
     gene            complement(4884..5507)
                     /locus_tag="VF995_08075"
     CDS             complement(4884..5507)
                     /locus_tag="VF995_08075"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9343552.1"
                     /translation="MRPESARRPRGRPRDARVDEAILEAALDLFIEAGFEGMSIEDVA
                     ERAGVARATVYRRWPSKQELVFAAIESCFEMLVPDSGEVQADLIAGVRQARHFLTETK
                     AGEALPRMVPEFAAGTPFGVAYLERVIRPRFQLVMEALARGQQRGELRDDLDLELALA
                     AIVGPMMFLRLTRRMREMGPDLPERIVRQAIEGLGPRTPSKPESGNG"
     gene            complement(5533..>6516)
                     /locus_tag="VF995_08080"
     CDS             complement(5533..>6516)
                     /locus_tag="VF995_08080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9343553.1"
                     /translation="LLSVAGLVSLVYGIIEAPNHGWTDPLILWAFGAGGALLLAFIGW
                     ELRAKHPMLQIAFFRNPRFSAASAAITLVFFALFGMIFLLQQHLQFVLGYSALQAGVR
                     LLPVATLVVAAPLAARVVEHAGTKLVVTAGLVIVATGLWLISRVGAGDGYAPIGWALA
                     VLGAGMGATMAPATASIMGSLPPAKAGVGSAMNDTTRQVGGALGVAVLGSVLSSAYHD
                     KITPALSRLPRPAASAARDSVGEALGVASRLGSSGSATGAAAGRHLAEVARGSFVHAM
                     NDVALVAAGVALLGAIVALTLLPARDGRPVAAAPADEEKEAALAATADEGE"
BASE COUNT          877 a         2532 c         2236 g          871 t
ORIGIN      
        1 tcgccgccac cctgctgcgg ttcgtgttgt tccgcagctg ggtgttccgc tccgcagccg
       61 caaccgcagc cgcaaccgcg accccgcccc cgcccaccgc aaccgcgacc accgcgaccc
      121 cccccgaccg cggcaaccgc ggcggtaccg ctgccgcctc catcaccgtc aatgcaaagg
      181 atccacccat gaccaccacg atccccaagc ccaccacctg gggggtggcc gacccggccg
      241 gcgggtccag gcgtgccggg ttgctccggc gcctggtccg cggccgggag agcgaccagc
      301 cctgggtccg ccccgcgctg ctggcgctgc tggcagcgac cgcgctgctg tacctgtggt
      361 cgctcggcgc ctccggctgg gccaacagct tctacgccgc cgccgtccag gccggcagca
      421 agagctggaa ggcgttcttc ttcggctcct ccgacgcctc caacttcatc accgtcgaca
      481 agacgccggc ctccctgtgg atcatggaca tctcggcgcg gatcttcggc gtcaactcct
      541 ggagcctgct ggtgccccag gcgctcgaag gcgtcgccgc cgtcgggctc ctctacgcgg
      601 ccgtgcgccg ctggttctcg gccggggcgg cgctgctggc cggcgcggtg atggcgctga
      661 cgccggtggc ggtgctgatg ttccgcttca acaaccccga cgcgctgctc accctgctgc
      721 tggtcgccgg cggctacgcg atggtgcggg cggtcgagga cggccgcacc cgctggctgg
      781 tgctggccgc gagctgcgtc ggcttcggct tcctcaccaa gatgctgcag gccctgctgg
      841 tggtgcccgc cttcgcactg gtctacctgg tggccgcccc gacgccgctg cggcgccgga
      901 tcggccagct gctcgccgcc ggggtcgcca tggtggtctc cgccggctgg tgggtggcgg
      961 ccgtcgcgct gattcccgcg gcgaatcggc cctacatcgg cgggtcgcag cacaacagca
     1021 tcctggagct gaccttcggc tacaacggct tcggccgcct caccggcaac gagaccggct
     1081 cagtcggcgg cggaggactc ggcggcaccg gaggccgctg gggcaccccc ggcctgttcc
     1141 gcctcttcag cgagatgggc ggggagatca cctggctgct ccccgcagcg gtggtcctgc
     1201 tcggcgccgg cctgtggctg acccgccgca tgccccgccc cgaccgcact cgcgcggccc
     1261 tgctgctgtg gggcggctgg ctggtggtca ccgggctgac gttcagctac gccgccggca
     1321 tcatccaccc ctactacacc gtggcgctgg cgcccgcgat cggcgcggtc gtcggcatcg
     1381 gcgcggcgtt cttctggcgc taccgctcct acctggtggc ccgcgcggtc ctcgccgcat
     1441 cgctggccgt caccggctgg tggtcctacg tcctgctgga gcgggcctcc ggctggcagc
     1501 cctggctggc cccgctggtg ctgctcggca gcctcgccgt cgccgccgtg ctgctcgccc
     1561 tgccgcacct gcgcctcgcc cgccgcgccg ccctggcggt ggccgccgcc ggcctgatcg
     1621 tcgcgctggc cgctcccgcc gcctactcga tggcgaccgc cgcgcagccc cacagcggcg
     1681 ccatcccctc ggtcggcccc gccagcgccg gcggcctcgg cttcgggcgc cccggcggcg
     1741 gcaacttcgg caccaaccgc cagttcggcg gcacccaggg ccagggccag gccccaggcg
     1801 gcttcgcctt cggcaccccc ggccagggac agggcaacgg caccaccaac ggccaaggtc
     1861 aggccagccc cttcggcggc cagggcaacg gcaacggcaa tggcaacggc aacggcggtt
     1921 tcggcggccg gggcggcgtc ggcagcctgc tcaacggcag caacccgagc agccagctgg
     1981 tcgccctcct gaagcagggc gccagcggct acacctggac cgccgcagcc gtcggctcca
     2041 actccgccgc cggcgtccag ctcgccaccg gcgagccggt gatggccatc ggcggtttca
     2101 acggcactga cccgaccccc accctcgccc agttccagca gtacgtccgc gacggcaaga
     2161 tccactattt catcgccagc ggcggcggca tgacgggcgg ccagggcggc tccagcacct
     2221 ccagccagat caccgcctgg gtcgagcaga acttcaagtc caccaccgcc ggcggcacca
     2281 ccgtctacga cctcagcacc gccagctcca ccaacacggc ataacgcaag aggaggtagg
     2341 ttgaccggcc ccggccgtcc cagcggcgac cggggccttc cttgtgcccg ctaccgccag
     2401 ggagctgcag cagcttctcc gtctcctccg cgcccgccac ctccaggttc aggtcgtggg
     2461 cgagctggac ggcgcggtcg accagcgggg cgttgccgtc ggtgagcttg cctttgccca
     2521 ggtggagggt gtcctccatg ccagcccggg cgttgccacc cagcgccagg ccaatcgccg
     2581 tcagcggcag gctcgcccgc ccgaccgcga tcacctgcca gatcgccccc gggggcaggc
     2641 ggcggaccat cgtgagcagg ttgtcggggg tggcggccat gccgccgcgg acgcccagga
     2701 ccatgctgaa ctggagcggc tcgtcgagca ggccctcatc gagcagccgc aggcaggcgt
     2761 ccaggtggcc ggtgtcgtag atctccagct cgggcttgac gcccagctcc cgcatgcggg
     2821 cggcgaggcg gcgcaccccg gcgggcgggt tgcggaactc gccctcccca aagctcatcg
     2881 agcaggggtt cagcgtggcc atgcggggcc gcagctccac cagccgctcg cgctcctcga
     2941 acgggaccga caggccgacg ccggtggaca gctggatcag gatcgggcag cgctcgctga
     3001 tcagctccat cgcccggcgg gcgatgccga ggtcggcggt gggccgctgc tgctcgtccc
     3061 ggaggtggac gtgcaccacc gaggcaccca gccggtgggc ctcctcggcg gcggcggcga
     3121 tctcctcagg ggtggtcggc agcgaggggt tgtcggcctt ggtcgcgatg gggccggtcg
     3181 gcgcgaccgt gatgacaacg ctcatacgtc ctccgagggg gcagcttgtt ccgcgacatg
     3241 gtcgtatcat cccgcattgc gcgcgagcgt agggccaccc caggaacagg tcaagcgagg
     3301 aggcagacag tcatgcggct ttcccaggtc ggtgcgctcc tggacccggc acccctcccg
     3361 atcgagaccg gctacgaacg cctccccgac ggcgtcctcc acgtcgccgc ccgcaccgac
     3421 ctgcacggct gcagcggcga gatgttcgag tggtggttcc gcttccgccc cgacacccag
     3481 cagtacatct ggtggcatcc ggtcgaccac cagttcagcg actggatcga gacccgcgag
     3541 ggcacccaca tcggctccat atcggctcca tccacgtggt cgtcgaggag ttcaccggcc
     3601 tgcccgccga gaagctctcc atccagttcc gcgaccccag cgagttcttc gaccccggcg
     3661 cctacgccgc cgcccgcgcc agcggcgcgg tcagcggcgc cgtcctcggc cgcgtcggcc
     3721 tgagccacac ccccgaccgc gaaccctccg gcgcgatcct cggcagccgc ctcctgcaca
     3781 tcgtccgcga caccccctgg ggcgccgccc tccgcagcca cttcttcctc ggccaggacc
     3841 tccccgccca gggcctctca cccgccgaga tcaccaccgc cttctccgac gagttcggcc
     3901 aggccctgct ccagcacgcc tacaacgagt tcacctttct ctcccgcttt ctgcccagcc
     3961 tctacatcgc cgagaaccgc gacaccaagc gccccgaagt cccctggtag ggaccgctca
     4021 ggcggtcgcg gcgccgaagg cgtcctcgac ggcgcggcgg gtgacgagca gcgtctcggc
     4081 gaggcgctgc tcgcggccgt ggaagcggag ggcgggcatg gggacgctga tcgccagggc
     4141 cgcgccggag gaggggagga ccgaggtgcc gacggcgcag atgccctcgg tgtgctcctc
     4201 gcggtcgaag gcgacgccgg aggcacgcac ctgggccagt tcggccagga gccgcttcgg
     4261 gtcggtgacg gtgtggacgg tgaaggccgg cagcgccggc ggcagcagcg cggccagctc
     4321 gtcgggcggg agggtggcca gcaccgcctt gccgttggcg gtgcagtgca gggggaacga
     4381 ggcgcccacc gccgacaccg cccgcagccg ctggggcgcc tcgacctggt ccaggaaggt
     4441 cacgtggtcg ccgtcgcgga tcgacaggtc cacggtctcg tccagctcgc gggagagctt
     4501 gtcgaggaag ggccggatct cggcgaccag cccgtggcgg gcgccctccg ccaagcggac
     4561 gaactccggc ccgagccggt agcggccccg ccgggtcctg ctcatcacca gcccttccgc
     4621 ctccagcgcg gtgaggatgc ggtgcacggt ggagcgcgcc agccccaccc tggcggcgag
     4681 ctctgcctgc gccagcccgc cggggctcgc ctgcagctcg cgcagcagct cggccgcccg
     4741 tgcgatcacc tggacgcccg ccgcgcgcga ctgccgctcc tgattggacc ggtccgtccc
     4801 catgcctgaa gccgcccccc ttcaccgcaa ctcccaccga gggtgccgcc gatggcgaaa
     4861 agccatgggc aacacggtac agactagccg ttcccagact ccggcttgct gggggtgcgg
     4921 ggtcccagac cctcgatggc ctgccggacg atgcgctcgg ggaggtcggg gcccatctcc
     4981 cgcatccgcc gggtgaggcg caggaacatc atcggcccga cgatcgcagc gagggcaagc
     5041 tccagatcga gatcgtcgcg gagctcgccg cgctgctggc ctcgtgccag cgcctccatc
     5101 acgagctgga agcgtggccg tatgacccgc tccaggtagg cgaccccgaa cggggtgccg
     5161 gccgcgaact ccggcaccat ccggggcaat gcctccccag ccttggtctc ggtgaggaag
     5221 tggcgggcct gccgcacccc ggcgatcaga tcggcctgga cctcgccgct gtcgggcacc
     5281 agcatttcga aacagctctc gatggcggcg aacaccagct cctgtttgga gggccagcgc
     5341 cggtagaccg tggccctggc gacaccggcc cgctcggcca cgtcctcgat gctcatgccc
     5401 tcgaagcccg cttcgatgaa caggtcgagc gccgcttcca ggatggcctc gtcgacacgg
     5461 gcgtcacgag gccgcccccg cggccgccgc gcggactcag gtcgcatccc cgcggccgag
     5521 ctcatgaccg catcattcac cctcgtcggc ggtggccgcc agggcagcct ccttctcctc
     5581 gtcggccggc gccgccgcga ccggccggcc gtcgcgggcg ggcagcaggg tcagcgcgac
     5641 gatcgcgccc agcagcgcca ccccggcggc gaccagggcg acgtcgttca tggcgtggac
     5701 gaacgacccc cgggcgacct cggccaggtg gcgcccggcc gccgcgccgg tggcggagcc
     5761 ggaggagccg agccggctcg cgacgcccag cgcctcgccg accgagtcgc gcgcggcgct
     5821 ggcggcgggc ctgggcagcc gcgagagggc gggggtgatc ttgtcgtggt aggccgacga
     5881 cagcacgctg cccagcaccg ccacgccgag cgcgccgccg acctggcggg tggtgtcgtt
     5941 catcgccgag ccgaccccgg ccttggccgg cggcagcgat cccatgatcg aggccgtggc
     6001 cggtgccatg gtcgcgccca tccccgcgcc cagcaccgcc agcgcccagc cgatcggcgc
     6061 gtagccgtcg ccggcgccga cccgggagat gagccacagc ccggtcgcca cgatcaccag
     6121 cccggcggtc accaccagct tggtgccggc gtgttcgacc acccgcgcgg cgagcggggc
     6181 cgcgaccacc agcgtcgcca ccggcagcag ccgcacgccg gcctgcagcg cgctgtagcc
     6241 gagcacgaac tgcaggtgct gctgcagcag gaagatcatg ccgaacagcg cgaagaacac
     6301 cagggtgatc gccgcgctgg cggcgctgaa gcgggggttg cggaagaagg cgatctgcag
     6361 catcgggtgc ttcgcccgca gctcccagcc gatgaacgcc agcagcaggg cgccgccggc
     6421 cccgaacgcc cacaggatca gcgggtcggt ccagccgtgg ttcggcgcct cgatgatccc
     6481 gtagaccagg ctcaccagcc cggcgaccga cagcag
//
LOCUS       DASWDC010000246         4759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1066723, whole genome shotgun sequence.
ACCESSION   DASWDC010000246 DASWDC010000000
VERSION     DASWDC010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066723"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VF995_08085"
     CDS             complement(<1..1247)
                     /locus_tag="VF995_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9343554.1"
                     /translation="MLRVTDMTVEIGHRLLVDETSFTISPGDVVGLVGPNGAGKTTLL
                     RTLAEAATASGASGKGDPRVQLVGALAWLPQESPPTSESTGLARLLSARGLDKTRDEL
                     ERARRRIEASQDQRARDRAIRRFSQLQELFEVEGGYRAEAEARRVAASLGVPPDGLDR
                     SLAGLSGGERRRVELARVLLSQAGTLLLDEPTNHLDTDAKRWLADFLADFPGGILAVS
                     HDLPLLDADVTGVLLLDPITASIESYKGTYTQYLKEREVRAASEARTRKVAERDIKRL
                     SAFVEKYRHANESMARRAMVTERRVERMKKVLTPVQRSGRKVAVKFPPPPLCGRIPLE
                     AEHLCRSYGGPMIFEDVSLDLERTERLLILGLNGAGKSSLLRILAGVDKPDSGEVRLG
                     HGAAIGYYAQEHEGLDPNKTSMEL"
     gene            1529..2056
                     /locus_tag="VF995_08090"
     CDS             1529..2056
                     /locus_tag="VF995_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343555.1"
                     /translation="MGAASRASASTADPTDTGDVTRHHAQVDWVSVRCVGDTLTGLVQ
                     ARGARGEVLTLELLERQRDGAKFVPAGRAAQVTLAARKRGNNHYHYSVGIGGLNARAY
                     QVTVRPSPRAALVRSPVVSAAECAPGPVVPEAALPILLPASMLVSLAAAGAVVTRRAR
                     RARRVRRGDPQPTSS"
     gene            2053..3762
                     /locus_tag="VF995_08095"
     CDS             2053..3762
                     /locus_tag="VF995_08095"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEX9343556.1"
                     /translation="MTVATQPGAGPIAERQALPWLVCVPLRLGLLVALLVLSYHYSLE
                     SLIKDWDYQAPLADLALVPPVALGLLVAATLRHQSVGIMRLGRADLVIGGAGLLIAAA
                     SMLLAPVVLTNYYWAFRPDLLSLPIATVAVVALLFGVRTVIPFAFPLAYLMLAWPLPH
                     AVMLEHAQRWLTTLTTGALRLLIGVVPVARVEAGGPDLRLFVPHVPGFTVGVASACSG
                     TGSLLGFSLIGLAALYLIDGPLRRRFAWLAVGALAIWVTNLLRVLAILAAGRLFGQSF
                     ALGVLHPLAGIVMVNLTFALLVIGLPRFGLTRRPLRAATPSDTPLTEPAPPEQRPTPR
                     QLLPRLAVLAVVAAVLAVANGQLAIASVGFEHAMQPVSATFDAQPRVGASWMAERLGQ
                     IDDARTYFGSTATWTRYRLTPLAGATAAPPQPFTVWVDSIVTSDLSALAAHPVLGCYR
                     LHGFKVLTAQRVTMVAGLIGELLAYRLPDGDVWHALSWEWPVRNPDGGILQERMTLLA
                     SSDLRAASPLPGSVGQGLRAPVLSAFNSLAPNHDPNPTLSRALLQVGAGIVASRTDLS
                     REG"
     gene            3769..>4759
                     /locus_tag="VF995_08100"
     CDS             3769..>4759
                     /locus_tag="VF995_08100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9343557.1"
                     /translation="MTSIGIYAMQGGLRIVFLVYGMLVIGHFLLQTLFAHLAWRRSLR
                     AAAIPQASSWQPSVDVVVACYNEDPDRLDACCRALLRQDYQGEVRVYLVDDGSGNREA
                     LVPVYERYAEYPGWEILLPENNAGKRHAQDLAVRFCRSDLIITIDSDTEIDPDGVRVI
                     VQPFRDERVGAVTGDVGVSNASANLLTRLIHMRYWVAFNQERAAQGYFGTVLCCSGPF
                     AVYRRSALAEVWSRYITQTFRGIACTYGDDRHLTNLVLSRDYLALFEPRAHAITHAPT
                     TLREYLRQQLRWNKSFYRELLWTLPFLLHRSRYMVFEVIVQTLLPLLLTLAIAT"
BASE COUNT          601 a         1756 c         1623 g          779 t
ORIGIN      
        1 agctccatgc tggtcttgtt ggggtcgagg ccctcgtgct cctgggcgta gtagccgatc
       61 gcggccccgt ggccgaggcg gacctcaccg ctgtcgggct tgtcgactcc ggcgaggatg
      121 cgcagcaggc tcgacttgcc ggcgccgttg agaccgagga tcagcagccg ctcggtgcgc
      181 tccaggtcga ggctgacgtc ctcgaagatc atcggcccgc cgtaggagcg gcagaggtgt
      241 tccgcctcca gcggaatccg gccgcagagc ggcgggggag ggaacttgac cgcgaccttg
      301 cggccggagc gctggaccgg ggtcagcacc ttcttcatgc gctcgacgcg ccgctcggtg
      361 accatcgcgc ggcgggccat ggactcgttg gcgtggcggt acttctccac gaatgcggag
      421 aggcgcttga tgtcgcgttc cgccaccttg cgggtgcggg cctcggaggc ggcgcggact
      481 tcgcgttcct tcaggtactg ggtgtaggtg cccttgtagc tctcgatcga ggcggtgatc
      541 gggtcgagca gcagcacccc ggtgacgtcc gcgtccagca gcggcaggtc gtgggagacc
      601 gccaggatgc cgccggggaa gtcggcgagg aagtccgcca gccagcgctt ggcgtcggtg
      661 tccagatggt tggtgggctc gtccagcagc agcgtgccgg cctgggacag cagcacccgg
      721 gccagctcca cccgccgtcg ctcgccgccg gagaggcccg ccagcgagcg gtcgaggccg
      781 tcggggggca cgccgaggga ggcggcgacg cggcgggcct cggcctcggc gcggtagccg
      841 ccctcgacct cgaacagttc ctgtaattgg gagaagcggc ggatcgcgcg gtcgcgggcg
      901 cgctggtcct ggctcgcctc gatgcgccgg cgtgcccgct ccagctcgtc cctggtcttg
      961 tcgaggccgc gggcggacag cagcctggcg aggccggtgg actccgaggt gggcggcgac
     1021 tcctgcggca gccaggcgag cgcgccgacg agctgcacac gcgggtcgcc cttgccgctg
     1081 gccccgctgg ccgtggcggc ctcggcgagg gtgcgcagca gggtcgtctt gccggctccg
     1141 ttgggtccca ccaggccgac gacgtcgccg ggggaaatcg tgaagctcgt ctcgtccacc
     1201 aggaggcggt ggccgatctc gacggtcatg tcagtgacgc gcagcatcgc gaacgggttc
     1261 cttcagtact gaatcgggtt gagcgtggcc gatcgtgcgg ttggcgccag gttcgggtca
     1321 agcgaattcc caggccacga gcttccttgc atggtagcgg agcctcggtt acagtcccgc
     1381 tcgaaccgtt aaagcaattc gaggaattca tctacttcag ctaggtagag ttgttctctc
     1441 tgtacctcta ccccccagga ggaatacaga cgtgcgctat ttccgatttc gtccagctct
     1501 tctcgtagcg cttgccctcc tcgtcccgat gggcgcggca agcagggcaa gcgcaagcac
     1561 tgccgacccg accgacaccg gcgacgtcac ccgccaccac gcacaggtcg actgggtcag
     1621 cgtccgctgt gtcggtgaca cgctcaccgg tctggttcag gcgcggggag ctcgcggaga
     1681 ggtcctgacg ctcgagctgc tcgagcggca gcgcgacggc gcgaagttcg tgccggccgg
     1741 cagggcggcg caggtgacgc tggccgcccg caaacgcggc aacaaccact accactacag
     1801 cgtcgggatc ggcggcctga acgcccgcgc ctaccaggtg acggttcgtc catccccgcg
     1861 ggcggcgctg gtgcgctcgc cggtcgtctc cgccgccgag tgcgcccccg gtccggtggt
     1921 ccccgaggcc gcactgccga tcctcctgcc ggccagcatg ctggtgagcc tcgccgccgc
     1981 cggggcggtg gtcacccgcc gtgcgcgccg tgcgcggcgg gtgcgccgcg gcgacccgca
     2041 gccgacctcg tcgtgaccgt ggcgacgcag cctggcgccg gcccgatcgc tgagcgccag
     2101 gctctcccat ggctggtgtg cgtgccgctc cgactcgggc tgctggtcgc gctgctggtg
     2161 ctctcctacc actactcgct cgagagcctc atcaaggact gggactacca ggctcccctc
     2221 gccgacctgg cgctggtccc gccagtcgcg ctcgggctgc tggtcgccgc gacgctccgc
     2281 caccagtcgg tcggcatcat gcggctcggc cgtgccgacc tggtcatcgg cggcgccggg
     2341 ctgctgatcg ccgcggccag catgctgctc gctccggtgg tgctgaccaa ctactactgg
     2401 gcgttccggc cagacctgct cagcctgccg atcgccacgg tggcggtggt ggcgctgctg
     2461 ttcggcgtcc gcaccgtgat ccccttcgcc ttcccgctcg cctacctcat gctcgcctgg
     2521 ccgctgccgc acgccgtgat gctcgagcac gcccagcgct ggctcaccac cctcaccact
     2581 ggggcgctcc gcctgctgat cggcgtcgtg ccggtggcca gggtggaggc gggcgggccg
     2641 gacctgcggc tgttcgtacc ccacgtcccg gggttcaccg tcggggtcgc cagcgcctgc
     2701 tcggggacgg gcagcctgct cggcttcagc ctgatcgggc tcgccgccct ctacctgatc
     2761 gacgggccgc tgcggcggcg gttcgcctgg ctcgcggtcg gcgcgctggc catctgggtc
     2821 accaacctgc tgcgcgtgct cgccatcctg gcggcgggcc gcctgttcgg gcagtccttc
     2881 gccctcggcg tgctgcatcc gctggccggg atcgtcatgg tgaacctgac cttcgccctg
     2941 ctggtcatcg gcctgccacg gttcggcctg acgcggcgcc cgctgcgcgc ggcgaccccg
     3001 agcgatacgc ctctcaccga gccggctccg cccgagcagc gtcccacccc gcgccagctc
     3061 ctgccccggc tggccgtgct cgccgtcgtc gccgccgtcc tcgccgtcgc caacggccag
     3121 ctcgccatcg cctcggtggg gttcgagcat gccatgcagc cggtcagcgc caccttcgac
     3181 gcccagcccc gcgtcggcgc cagctggatg gcggagcgcc tcggccagat cgacgacgcc
     3241 cgtacctact tcggcagcac cgccacctgg acccgctacc gcctgacccc gctggccggc
     3301 gccacggccg cgccgccgca gccgttcacc gtgtgggtgg actcgatcgt caccagcgac
     3361 ctgagcgcgc tcgccgctca cccggtgctg ggctgctacc ggctgcacgg cttcaaggtg
     3421 ctgaccgccc agcgggtcac catggtcgcc ggcctgatcg gcgagctgct cgcctaccgg
     3481 cttcccgacg gcgacgtctg gcatgccctg agctgggaat ggccggtgcg caaccccgac
     3541 ggcgggatcc tgcaggagcg catgacgctg cttgcctcca gcgacctgcg cgccgcgagc
     3601 cccctgcccg gcagcgtggg tcagggcctg cgcgcccccg tgctcagcgc gttcaactcg
     3661 ctcgccccca accatgatcc caacccgacg ctctccaggg cgttgcttca ggtcggggcc
     3721 ggcatcgtcg ccagccgtac ggatctctcc agggagggct gatcagttgt gacctcgatc
     3781 gggatctatg ccatgcaggg cgggctcagg atcgtgttcc tggtctatgg gatgctcgtg
     3841 atcgggcact tcctgctcca gaccctgttc gcgcacctgg cctggcggcg cagcctgcgc
     3901 gccgcggcga tcccccaggc gtccagctgg cagccgagcg tcgacgtggt cgtggcctgc
     3961 tacaacgagg accccgaccg gctcgacgcc tgctgccggg ccctcctgcg ccaggactac
     4021 cagggggagg tgcgcgtcta cctggtcgac gacggctcgg gcaaccgcga ggccctggtg
     4081 ccggtctacg agcgctacgc cgagtatccc ggctgggaga tcctgctgcc cgagaacaac
     4141 gccggcaagc gccacgcgca ggacctggcg gtgcggttct gccgcagcga cctgatcatc
     4201 accatcgact ccgacaccga gatcgacccg gacggcgtcc gcgtgatcgt gcagcccttc
     4261 cgcgacgagc gcgtcggcgc ggtcaccggc gacgtcggcg tctccaacgc ctcggccaac
     4321 ctgttgaccc gcctgatcca catgcgctac tgggtcgcct tcaaccagga acgcgccgcg
     4381 cagggctact tcggcacggt gctgtgctgc tccggcccgt tcgcggtcta ccgccgctcg
     4441 gccctggccg aggtgtggtc gcgctacatc acccagactt tccgcggcat cgcctgcacc
     4501 tacggcgacg accgccacct gaccaacctg gtgctgtcgc gcgactacct ggctctgttc
     4561 gagccgcgcg cgcacgccat cacccacgcg cccaccaccc tgcgggagta cctgcgccag
     4621 cagctgcgct ggaacaagag cttctaccgg gagctcctgt ggacgctgcc gttcctgctc
     4681 caccgctcca ggtacatggt cttcgaggtg atcgtccaaa cgcttctgcc gctactcctc
     4741 acgctcgcga tcgcgacgt
//
LOCUS       DASWDC010000247         4414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1398100, whole genome shotgun sequence.
ACCESSION   DASWDC010000247 DASWDC010000000
VERSION     DASWDC010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398100"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VF995_08105"
     CDS             complement(<1..546)
                     /locus_tag="VF995_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX9343558.1"
                     /translation="MRVAVIVCDSLGVGAAPDADAYGDEGANTLGHTAAAVGGLALPT
                     LGSWGIGLLTEVAGVPPADPPLGVVARMAERSAGKDTTTGHWEMMGVVLQTPFPTYPH
                     GFPAEVIEPFEAAIGRRVLGNRPASGTEIIKELGEAHLASGDPIVYTSGDSVFQIATH
                     KRVVPLEQLYEWCETARKLLTG"
     gene            complement(558..941)
                     /locus_tag="VF995_08110"
     CDS             complement(558..941)
                     /locus_tag="VF995_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX9343559.1"
                     /translation="MDQQTTQQLRERLAGEQGALRKQLRELGARPDAEGIEEPDLDAG
                     FADAGQAAAERSSLLTLVRKLRTQLVEVEGALVRIDAGTYGRCEKCGQPIPEERLEAL
                     PAVRLCMSCKRATTGSGRTMGSGRR"
     gene            complement(1036..2031)
                     /gene="xerD"
                     /locus_tag="VF995_08115"
     CDS             complement(1036..2031)
                     /gene="xerD"
                     /locus_tag="VF995_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX9343560.1"
                     /translation="MPTTADVAIRRYLDYLAVERGLAANTLAAYRRDLRRYAAWLASS
                     GIDDPAAASEEDLVAFLGALRVALTPVGTPYQATSVARSLAAVRGFHRFLVREHLAGA
                     DPSAELGTPKLPVALPKALSVEQVEALLGAVAGDTALALRDRALLETLYAAGLRVSEA
                     TALDVDDLDLEDGSLRAFGKGSKERLLPVGRSACRALQAWLVRGRPQLARVRSGPALF
                     LNAQGSRLTRQGCWKLLQRHARHAGLEARVSPHVLRHSFATHLLAGGADIRSVQELLG
                     HASLATTQVYTKLTQERLREVYLRAHPRARMPRARTPQTPRARTAPTPEILARYG"
     gene            complement(2037..3200)
                     /gene="ald"
                     /locus_tag="VF995_08120"
     CDS             complement(2037..3200)
                     /gene="ald"
                     /locus_tag="VF995_08120"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX9343561.1"
                     /translation="MHIGIPRELKDHEYRVAITPIGVRELTHAGHQVLIEQDAGTGSA
                     IPDRDFERAGARVLPSADEVWGQSDLLLKVKEPIAEEFGRLRKDQVLFTYLHLAASAE
                     VTSALASAGTTAVAYETVELPDGRLPLLAPMSEVAGRMAPQVGAHLLEREQGGRGVLM
                     GGVSGVGAAKVVVFGGGMAGQNAAWIAAGMEAEVTLLDTSVDKLRYVDSIHKGRIVTL
                     MSNRSTVEDLIRAADLAIGAVLIPGARAPRLVSREMVSEMKAGSVLVDISIDQGGCFE
                     TSHVTTHSDPTYVVSDVVHYCVGNMPGAVPHTSTYALTNVTLPYVLQIAAHGLEAAVR
                     TDPALAKGVNVYQGELCYQPVAEAHGMEWRPLADLLPDLLPGGVAKGGRKGDG"
     gene            complement(3321..3959)
                     /locus_tag="VF995_08125"
     CDS             complement(3321..3959)
                     /locus_tag="VF995_08125"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9343562.1"
                     /translation="MPPEPPYRALDRTRPYQGKLVSVRVDHLASPSGETTTREVVEAR
                     GAVAGLAVDEQGRVLLVEQWRNAPGGRLVEIAAGQYDVDGEAVEAALRRELAEELGVG
                     GGTATWLASVHTTPGWSDEVVDLFLVEGVRPLSPRERGKVAKGVWEEAELRVLRLGLE
                     EAMALAATPPGDAKTLLALALYGLKHAGVWTSDQAGRPAPDAPARRAAARPG"
     gene            complement(4070..>4414)
                     /locus_tag="VF995_08130"
     CDS             complement(4070..>4414)
                     /locus_tag="VF995_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343563.1"
                     /translation="AQRGAQRGAQRGAQRDGQGQDGRRPSDRAPLAPTSSAARQDPHP
                     AAAPPRGPQERIVDVREREADVPAPASASTRPPGLLPPAGSGLFAPPSARNSNGMAER
                     ADGYGDGYGDRL"
BASE COUNT          536 a         1638 c         1634 g          606 t
ORIGIN      
        1 cccggtgagg agcttccggg cggtctcgca ccactcgtag agctgctcca gcggcaccac
       61 gcgcttgtgg gtggcgatct ggaacaccga gtcaccggag gtgtagacga tcgggtcgcc
      121 gctggcgagg tgcgcctcgc cgagctcctt gatgatctcg gtgccggacg cggggcggtt
      181 gccgagcacc cggcggccga tcgccgcctc gaacggctcg atgacctcgg cggggaagcc
      241 gtgcgggtag gtcgggaagg gcgtttggag gaccacgccc atcatctccc agtgcccggt
      301 ggtggtgtcc ttgccggcgg aacgctccgc catgcgggcg accaccccga gcggcgggtc
      361 ggccggcggc accccggcaa cctcggtgag caggccgatc ccccacgacc cgagggtcgg
      421 cagcgccagc ccgccgacgg cggcggcggt gtgcccgagg gtgttggcgc cctcgtcgcc
      481 gtaggcgtcg gcgtccggcg cggcaccgac cccgagcgag tcgcagacga tcaccgcgac
      541 cctcatccct ggagcgctca ccggcgcccg gaccccatgg tccgtcccga cccggtggtc
      601 gcgcgcttgc agctcatgca gagccgcacc gccggcagcg cctccaggcg ctcctccggg
      661 atcggctgtc cgcatttctc gcagcgcccg taggtgccgg cgtcgatccg gacgagcgcg
      721 ccctcgacct cgaccagctg ggtgcgcagc ttgcggacca gggtgagcag gctggagcgc
      781 tccgccgccg cctggccggc gtcggcgaag cccgcgtcca ggtctggctc ctcgatcccc
      841 tcggcgtcgg ggcgtgcccc aagctcgcgg agctgcttac gcagcgcgcc ctgctccccg
      901 gccagccgct cgcgcagctg ctgggtcgtc tgctggtcca tctttgctgc tcctctgctg
      961 gccactaggc ggccgtgtcc aacacgccgg acacgaggga tcgaactcga aggaaacgct
     1021 acaagaccat ccaggtcaac cgtatcgagc aagaatttct ggagtgggtg ccgtccgcgc
     1081 ccgcggcgtc tgcggcgtcc gcgccctcgg catcctcgcc ctggggtggg cgcgcaggta
     1141 gacctcgcgc agccgctcct gcgtcagctt ggtgtagacc tgggtggtgg ccaggctcgc
     1201 gtggccgagc agctcctgca cgctgcggat gtcggccccg ccggccagca gatgggtggc
     1261 gaaggagtgg cgcagcacgt gcggggagac ccgcgcctca agccccgcgt ggcgggcgtg
     1321 gcgctgcagg agcttccagc atccctgccg ggtcagccgg ctgccctgcg cgttgaggaa
     1381 cagcgccggc ccagagcgga cccgggcgag ctgcggccgc ccgcggacca gccacgcctg
     1441 cagcgcccgg caggcggacc gccccaccgg cagcagccgc tccttggagc ccttgccgaa
     1501 ggcgcgcagg ctgccgtcct ccaggtccag gtcgtcgacg tccaacgcgg tcgcctcgct
     1561 gacccggagc cccgccgcgt acagcgtctc gagcagcgcg cggtcccgca acgccagcgc
     1621 cgtgtccccg gcgaccgcgc cgagcagcgc ctccacctgc tcgaccgaca gcgccttcgg
     1681 cagtgcgacc gggagcttcg gggtgccgag ctccgccgac ggatcggcgc cggcgaggtg
     1741 ctcacggacc aggaagcggt ggaagccacg caccgccgcg aggctgcgcg cgacgctggt
     1801 cgcctggtac ggggtgccga ccggggtgag cgccacccgc agcgcgccga ggaaggcgac
     1861 caggtcctcc tcgctggccg cggccgggtc gtcgatgccg ctggaggcca gccacgcggc
     1921 gtagcggcgc aggtcccgcc ggtaggcggc gagggtgttg gcggccaggc cccgctccac
     1981 cgccaggtag tccaggtagc ggcggatcgc cacgtcagcg gtggtgggca tggcggtcag
     2041 ccgtcgcctt tgcggccgcc tttggcgacg ccgccgggga gcaggtcggg gagcaggtcg
     2101 gcgagcgggc gccactccat gccgtgggcc tcagcgaccg gctggtagca gagctcgccc
     2161 tggtagacgt tgacgccctt ggccagcgcc gggtcggtcc gcacggcggc ctccagccca
     2221 tgcgcggcga tctggagcac atagggcagg gtgacgttgg tgagggcata ggtggaggtg
     2281 tgcggcaccg cccccggcat gttgccgacg cagtagtgga ccacgtcaga gacgacgtag
     2341 gtcgggtcgg agtgggtggt gacgtgcgag gtctcgaagc atccgccctg gtcgatggag
     2401 atgtccacca gcacgctgcc cgccttcatc tcgctgacca tctcgcggga gaccagccgc
     2461 ggcgcccgcg ccccggggat cagcaccgcg ccgatcgcca ggtcggcggc gcggatcaga
     2521 tcctcgacgg tcgagcggtt cgacatcagc gtgacgatgc ggcccttgtg gatcgagtcg
     2581 acgtagcgga gcttgtcgac gctggtgtcg agcagggtca cctcggcctc catgccggcg
     2641 gcgatccagg cggcgttctg gccggccatg ccgccgccga acaccaccac cttggccgcg
     2701 ccgacgccgg agaccccgcc catcagcacg ccgcggccgc cctgctcgcg ttccagcagg
     2761 tgcgcgccga cctgcggagc catgcggccg gccacctcgc tcatcggggc gagcagcggc
     2821 aggcgcccgt cgggcagctc caccgtctcg taggcgaccg cggtggtgcc ggcgctggcc
     2881 agcgcgctgg tgacctcggc gctggcggcc aggtgcaggt aggtgaacag cacctggtcc
     2941 ttacgcaggc ggccgaactc ctccgcgatc ggttccttga ccttgagcag caggtccgac
     3001 tggccccaga cctcgtcggc gctgggcagg actcgcgcgc ccgcgcgctc gaagtcacgg
     3061 tcggggatcg cgctgccggt cccggcgtcc tgctcgatca gcacctggtg gccggcatgg
     3121 gtcagctccc gtaccccgat tggggtgatc gccacccggt actcgtggtc cttcagctcg
     3181 cgcgggatcc cgatgtgcat cgtgcgcgcc ctttccgtca accggcctcc gttggccgcc
     3241 caatgtcagt ctacgagagt gaacccttcc ggcggcaggg cccatctcct cagcgcggcg
     3301 ttccggccgc ggcgtggtcg tcagccgggc cgcgccgcgg cgcggcgggc gggagcgtcc
     3361 ggggcgggtc ttcccgcctg gtcggacgtc cacaccccgg cgtgtttgag cccgtagagg
     3421 gcgagcgcga gcagcgtctt ggcgtcgccc ggcggtgtgg cggcgagcgc catcgcctcc
     3481 tcgaggccga gccggaggac acgcagctcg gcctcctccc agacaccttt ggcgaccttg
     3541 ccccgctccc gcggcgagag cgggcgcacg ccctccacca ggaacaggtc gacgacctcg
     3601 tcgctccagc cgggcgtggt gtggacactg gcgagccagg tggcagtgcc gcctccgacg
     3661 cccagctcct cggccagctc gcggcgcagc gccgcctcca ccgcttcgcc gtcgacgtcg
     3721 tactggccgg cggcgatctc gaccaagcgc ccgccggggg cgttgcgcca ctgctcgacc
     3781 agcagcaccc gcccctgctc gtccacggcc aggccggcga ccgcgccacg cgcctccacc
     3841 acctcgcggg tggtggtctc gccgctggga ctggcgaggt ggtcgacccg gacgctgacg
     3901 agtttgccct ggtagggacg ggtgcggtcg agggcgcggt aggggggttc ggggggcacg
     3961 gtcccgcggc cggaatgacg acgcgccgat tccacggttg ccgcggggcc ccccgatggg
     4021 tcggggggtt cggggggcac gccggcccga cgctccccct ccccgctgct cacaaccggt
     4081 cgccgtaccc gtcgccgtac ccgtcggcgc gctcggccat gccgttgctg tttcgcgccg
     4141 acggcggggc gaacaggccg gagccggccg gtgggagcag ccccggcggg cgggtcgacg
     4201 cggatgccgg ggcaggcacg tccgcctccc gctcccggac gtcgacgatg cgctcctgcg
     4261 gaccccgggg cggagccgcc gccgggtgcg ggtcctgccg ggccgccgag ctggtgggcg
     4321 cgagcggtgc ccggtccgac ggccgcctgc cgtcctgccc ctgcccgtcg cgctgggcgc
     4381 cgcgctgggc gccgcgctgg gcgccgcgct gggc
//
LOCUS       DASWDC010000248         6454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1317272, whole genome shotgun sequence.
ACCESSION   DASWDC010000248 DASWDC010000000
VERSION     DASWDC010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317272"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VF995_08135"
     CDS             <1..310
                     /locus_tag="VF995_08135"
                     /inference="COORDINATES: protein motif:HMM:NF040132.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343564.1"
                     /translation="GVGFHAASAPSHGIVPIYRLHNHTTGDYVYTASTAERTSLTHAG
                     YVNEGPVFYAPTTPAPGLYPAFRLQRGLYHRIAFGTLARNDAIAAGWHLEHAIFYAHR
                     "
     gene            complement(412..1302)
                     /locus_tag="VF995_08140"
     CDS             complement(412..1302)
                     /locus_tag="VF995_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX9343565.1"
                     /translation="MTVAVITDGAASLPDELARDRGVTVVPLEVTIGGHAAGDGGPVP
                     LEEVVRRLGDGVRTSSPPPGRFAAAIRRSQDQDQGRGGDGVLILTVARSMSSTYQAAA
                     LAARQAAGASGPGFGPVEVLDTGTAAGGQGLVVLAAAQAAQRGRPLGEVAAAARTAAG
                     RVRLVATLDNLDQLVAGGRLPRAVGRLGTLLGVQPLFEFSHGGIHPRAPAFSRAAARR
                     RILARWRSSRPRGPAALHVAALHALAPHEAESLLGAVRAELGTDPATAFIGEFGAAMV
                     AHTGPNLTGLSWYWEGEPRV"
     gene            complement(1299..2690)
                     /locus_tag="VF995_08145"
     CDS             complement(1299..2690)
                     /locus_tag="VF995_08145"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9343566.1"
                     /translation="MAERPNVLSRPGFRSLAIGQAVSAFGDWMGTVALMALVLDLTGS
                     ATAVGVILVLRLLPAGFAGPIAAGLAERWDRRRIMLTADLLRAGLVALVPLVRAAWWV
                     YLWAFLLEVLNIVFLPARDASIPDLVDEDDLPTANGIILGSSYGNIPIGAGAFALLTL
                     IPTGWNGWLDAHPNSLALWIDAATYLVSFAFIRRLAVLGPATGASAEAAEAEGEEGVG
                     RDTLAGIRAALSLPIVRAVMPATVTAVVGIGALFSLGVVFVREVLHASTGEFGGMVAL
                     FGVGGVAGWAFLQRQEDGPTLATIKLATGAMGVILAVMSLLSHVVLAYLAAIPFGAAG
                     AAALVASLTYVQEALTGDQRVLGLTVYHVVLRVGMSVAAIGGGLAAQWVGPVGVPLLG
                     TLQPSALVMFCAGVVMVLGAAFMPTTVPDASGSPASNAAAEGPAGEAGPAAEQREGQP
                     RGTGGAHGPKAAS"
     gene            complement(2690..3301)
                     /locus_tag="VF995_08150"
     CDS             complement(2690..3301)
                     /locus_tag="VF995_08150"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX9343567.1"
                     /translation="MRTEDAAKLGIAFLIGAIPFSNLAARLTKGVDLRAVGSGTVSGT
                     SLYRVAGWGPLAAAGVLEVGKGAVGPLLVGRQRRELAALAAGLAVAGHNWSPFLRGAG
                     GRGISPSLGALGVLAPPGAALLFGGMALGKLAGQAAPGSLAAQLALVPLLRRTNGRAG
                     ALAGAAVLAPLLAKRLLGNAPPPAGSPAVRVYLTRLLHDRDVA"
     gene            3599..4426
                     /gene="hpnC"
                     /locus_tag="VF995_08155"
     CDS             3599..4426
                     /gene="hpnC"
                     /locus_tag="VF995_08155"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX9343568.1"
                     /translation="MAKAGGENFPVAPVLLGRRLRGHLLAVYGFARLVDDLGDEAKGD
                     RLAQLDLFEQDLARIWSGTPRDPLLVRLQPTVREFSIPPEPFQRLIEANRQDQRVSRY
                     QTYQELLDYCVLSANPVGHLVLYVFGKATPERLRLSDAVCTGLQLVEHWQDVAEDLAR
                     DRVYLPREDLAAFGCDERDLAALHASGRLRKLLAFEAGRAAALLDEGAPLIGLLTGRA
                     RLAVTGFLAGGRAALAAIADAGYDVLPGPPKPSRPRLVREAVSVLLRNRNRKRKGKG"
     gene            4436..5386
                     /locus_tag="VF995_08160"
     CDS             4436..5386
                     /locus_tag="VF995_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX9343569.1"
                     /translation="MDVFEAYRQCERVTRERARNFYYGIRLLPPAKRSALCAVYALAR
                     RVDDIGDGQLPDGEKLAQLATVRAGLGSLAGDPSEAEGDDDDPVLVALSHATRHFPIP
                     LTAFGELVDGVELDVRGTTYPTFDELAHYCRCVAGSIGRLSLGVFGSADPPEADRLAD
                     ALGLAMQLTNILRDLREDAAAGRVYLPAEDLARFGWDPLGDFTGGLPLGRRRPSDAVA
                     GASLLSPPDPRRWEPDERLTGLISFQAARAQRYFSEGLGLVPMLDRRSAACVMAMAGI
                     YQRLLDRITRRPELVLRQRVSLHAWEKAEIAVRSLTRMPA"
     gene            5335..>6454
                     /locus_tag="VF995_08165"
     CDS             5335..>6454
                     /locus_tag="VF995_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9343570.1"
                     /translation="MGEGRDRGAEPDEDAGMTGAEVAPTVVVVGGGLAGITAALVAAD
                     AGAQVTLLEARARLGGATFSFQRDGLWIDNGQHVFLRCCTAYRWLLRRLGAEDLVTLQ
                     RRLALPVVAPGGRVGWLRRGALPLPPPLHLAGALARYPYLSWPQRLRVGRAALAMRRL
                     DPADEANDRQALAPWLAAHGQDAGSLQALWELISLPTLNARAVDSSLALGLKVFKTGL
                     LEAADSADIGYARVPLQQLHGERAEKALGDAGARLRTHCRVSKIEPLGGPEGGFAVMP
                     GEGERLEAAAVILAVPHDGTGGLLPTAAEPDRLGALGALGSSPIVNLHVRYDRRVTTL
                     DFAAGLRTPVQWVFDRTAASGLDPAWGDGQHLAVSLSAA"
BASE COUNT          870 a         2399 c         2398 g          787 t
ORIGIN      
        1 cggcgtgggc ttccacgcgg cctcagcgcc ctcgcacggg atcgtgccga tctacaggct
       61 gcacaaccac accaccggcg actacgtcta caccgccagc accgcggaac gaacttcgct
      121 gacccacgcc ggctacgtca acgaaggccc cgtcttctac gccccaacca ccccagcccc
      181 aggcctctat cccgccttcc gcctccagcg aggcctctac caccgcatcg ccttcggaac
      241 cctggcccgc aacgacgcca tcgcggccgg ctggcacctg gaacacgcca tcttctacgc
      301 ccaccgctga cccagccagc caccccactc gacgccccga ccccactcga cacctccata
      361 ggggcgagct gtgagcccgc gccgccggat ccaccccgcc aaggcgcagg ctcacacccg
      421 gggttcccct tcccagtacc agctcagccc ggtgaggttc ggcccggtgt gggcgaccat
      481 ggccgcgccg aactcgccga tgaaggcggt ggccgggtcg gtgccgagtt cggcgcggac
      541 ggcgccgagc agcgactcgg cctcgtgcgg ggcgagggcg tggagcgctg cgacgtgtag
      601 agccgctggc ccccggggcc gggaggagcg ccagcgggcg aggatgcggc ggcgggcggc
      661 cgcgcgggag aaggccgggg cgcgcgggtg gatgccgccg tgggagaact cgaagagcgg
      721 ctggacgcca agcagggtgc cgaggcggcc gaccgccctg gggaggcggc cgcccgcgac
      781 cagctggtcg aggttgtcga gggtggcgac caggcgcacc cgcccggcgg cggtgcgggc
      841 ggcggcagcg acctcgccga gggggcggcc ccgctgagcg gcctgggccg ccgcgaggac
      901 caccaggccc tggccgccgg cggccgttcc ggtgtcgagg acctcgaccg ggccgaagcc
      961 gggcccggag gcaccagcgg cctggcgggc ggcgagggcg gcggcctggt aggtactgct
     1021 catcgagcgt gccacggtga ggatcaggac gccgtcgccg ccgcgaccct ggtcctggtc
     1081 ctggctccgc ctgatcgcgg cggcgaagcg gcccggcggc gggctggagg tacggacccc
     1141 gtcgccgagg cgccgcacca cctcctccag cggcaccggc ccgccgtccc cggccgcgtg
     1201 gccgccgatg gtcacctcca gcggcaccac ggtcaccccc cggtcacgcg ccagctcgtc
     1261 agggagggag gcggcgccgt cggtgatgac cgcgacggtc acgatgccgc cttcgggccg
     1321 tgcgccccgc cggtccccct gggctggccc tcgcgctgct cggccgctgg tccagcctcc
     1381 cccgctggcc cctcggccgc ggcgttggac gcggggctcc ccgacgcgtc agggacggtt
     1441 gtgggcatga aggccgcgcc gaggaccatc accaccccgg cgcagaacat caccagcgcg
     1501 gagggctgga gggtgccgag cagcggcacg cccaccgggc cgacccactg ggcggcgagg
     1561 ccgccgccga tcgcggcgac gctcatcccg acccgcagga ccacgtggta gacggtgagg
     1621 ccgaggacgc gctggtcgcc ggtgagcgcc tcctggacgt aggtgaggct ggcgaccagc
     1681 gccgcggcgc ccgccgcgcc gaacgggatc gccgccaggt aggcgagcac cacgtgcgac
     1741 agcaggctca tcaccgcgag gatcacgccc atcgcgccgg tggcgagctt gatcgtggcc
     1801 agggtcggcc cgtcctcctg gcgctgcagg aacgcccacc cagccacccc gccgacgccg
     1861 aacagcgcca ccatgccgcc gaactccccg gtgctggcgt gcagcacctc gcgcacgaac
     1921 accaccccga gcgagaacag cgcgccgatg ccgaccacgg cggtgacggt ggcgggcatg
     1981 accgcccgca cgatcggcag gctcagcgcg gcccggatcc cggcgagggt gtcccgcccc
     2041 acgccctcct caccttcggc ctcggcagcc tcggcgctgg cgccggtcgc cgggccgagc
     2101 accgcgaggc ggcggatgaa ggcgaacgac accaggtagg tggccgcgtc gatccacagc
     2161 gcgagcgagt tggggtgcgc gtccagccac ccgttccagc cggtcgggat cagggtgagc
     2221 agggcgaacg ccccggcgcc gatggggatg ttgccgtagg aggagccgag gatgatgccg
     2281 ttggcggtcg gcaggtcgtc ctcgtcgacc aggtcgggga tggaggcgtc gcgcgccggc
     2341 aggaacacga tgttgagcac ctccagcagg aaggcccaca ggtagaccca ccaggcggcc
     2401 cgcaccagcg gcaccagggc gaccagcccg gcgcgcagca ggtcggcggt gagcatgatg
     2461 cggcgccggt cccagcgctc ggccagcccg gcggcgatcg gcccggcgaa cccggccggc
     2521 agcaggcgca gcaccaggat caccccgacc gcggtggccg acccggtcag gtcgaggacc
     2581 agggccatca gcgcgaccgt gcccatccag tcgccgaacg ccgacaccgc ctgcccgatc
     2641 gccagggagc ggaacccggg gcggctgagg acgtttggcc gctccgccat caggccacgt
     2701 cccggtcgtg cagcaggcgg gtgaggtaca cgcgcaccgc cggcgacccg gccggcgggg
     2761 gcgcgttgcc gagcagccgc ttggcgagca gcggcgccag caccgccgcc ccggccagcg
     2821 cgccggcacg gccgttggtg cggcgcagca gcgggaccag ggcgagctgc gccgccagcg
     2881 acccgggggc ggcctggccg gcgagcttgc cgagggccat cccgccgaac agcagcgcgg
     2941 cccccggcgg cgcgagcacc ccgagcgcac ccagcgacgg ggagatcccc cggccgccgg
     3001 cgccgcgcag gaacggcgac cagttgtgcc cagcgacggc gagcccggcc gccagtgccg
     3061 ccagctcgcg ccgctggcgg ccgaccagca gcgggccgac cgcgcccttg ccgacctcca
     3121 gcaccccagc ggcggccagg ggcccccagc ccgccacccg gtacaggctg gtaccggaga
     3181 ccgtgccgct gccgaccgct cgcaggtcga cgcctttggt gagccgggcg gccaggttcg
     3241 agaaggggat cgcgccgatc aggaacgcga tgcccaactt ggcggcgtcc tcggttcgca
     3301 ctcggagacc tccctttgcc tccccggcag cgaagggtaa tcccgctaaa cgtaccggaa
     3361 gacgtcgaca tgacgacgac atgccgaacc aaccgcgtaa tgagcttggc tcgatccgca
     3421 tgataggcct aggctggttt tcgccgaacg gatcgccaat tcgcctccca tggtccgttt
     3481 ggttgctcga cgcgcatgtt gccttgcagt ggcgattccg aatggggaag cgggcctctt
     3541 gcctgaatcg ctcgaaccgg ccataagtag ctccgcggtc gggtcagaac acggggtaat
     3601 ggccaaggct ggcggggaga acttccctgt cgccccggtc ctgctcggcc ggaggctgcg
     3661 cggccaccta ctggccgtgt atggcttcgc gcggctggtc gacgacctgg gggacgaggc
     3721 caaaggggac cgcctcgccc agctcgacct gttcgagcag gacctcgcgc ggatctggag
     3781 cggcaccccg cgcgaccccc tgctggtgcg gttgcagccg acggtgcggg agttctcgat
     3841 ccctccggag ccgttccagc gcctgatcga ggccaaccgg caggaccagc gggtcagccg
     3901 ctaccagacg taccaggagc tgctcgacta ctgcgtgctg tcggccaacc cggtcggcca
     3961 tctggtgctg tacgtgttcg ggaaggccac gccggagcgg ctgcggctgt cggacgcggt
     4021 ctgcaccggc ctgcagctgg tcgagcactg gcaggacgtg gccgaggacc tcgcccgcga
     4081 ccgcgtctac ctgcccaggg aggacctggc cgcgttcggc tgcgacgagc gggacctggc
     4141 ggcgctccac gcgagcgggc ggctgcggaa gttgctggcc ttcgaagccg gccgggccgc
     4201 cgcgctgctc gacgagggcg ccccgctgat cggcctgctt acgggacgcg cccgcctggc
     4261 tgtgacaggc tttctcgctg gtggccgcgc tgccctggcc gcgattgccg acgccggcta
     4321 cgacgtcctg cccggaccac cgaagccgtc ccgcccgagg ctggtgcgtg aggcggtgag
     4381 cgttctcctg cgaaaccgga accggaagcg gaaggggaag gggtgagcaa gtagtttgga
     4441 cgtcttcgaa gcctaccggc agtgcgagcg ggtgacccgc gaacgggccc gcaacttcta
     4501 ttacggcatc cggctgctgc cccccgccaa gcgaagcgcc ctgtgcgcgg tctacgcgct
     4561 ggccaggcgg gtcgacgaca tcggcgacgg gcagctcccc gacggcgaga agctggccca
     4621 gctcgcgacg gtccgcgccg ggctcggcag cctggcaggc gacccctccg aggccgaggg
     4681 ggacgacgac gatccagtgc tggtggcgct gtcgcacgcg acccggcact tccccatccc
     4741 gctgaccgcc ttcggcgagc tggtcgacgg cgtcgagctg gacgttcgcg gcaccaccta
     4801 cccgaccttc gacgagctgg cgcactactg ccgctgtgtg gccgggtcga tcgggcggct
     4861 gtcgctgggg gtgttcggct cggccgaccc gcccgaggct gaccggctgg ccgacgcgct
     4921 cggcctcgcc atgcagctca ccaacatcct gcgggacctg cgcgaggacg ccgccgccgg
     4981 gcgtgtctac ctgcccgccg aggacctggc ccgcttcggc tgggacccgc tgggcgattt
     5041 caccgggggt ctaccgctcg gccgtcgtcg gccgtcggat gctgtggccg gggcctcgct
     5101 cttgagcccc ccggaccccc ggcgctggga gccggacgag cggctcaccg ggctgatcag
     5161 cttccaggcg gcccgcgcgc agcggtattt cagcgagggc ctgggcctcg tcccgatgct
     5221 cgaccggcgc agcgccgcct gcgtcatggc gatggccggc atctaccagc ggctgctcga
     5281 ccgcatcacc cggcggccgg agctggtcct gcgccagcgg gtctcgctgc acgcctggga
     5341 gaaggccgag atcgcggtgc ggagcctgac gaggatgccg gcatgaccgg cgcggaggtg
     5401 gccccgactg tcgtggtcgt tgggggtggg cttgctggga tcaccgccgc gctggtcgcg
     5461 gcggacgcgg gcgcccaggt gaccctgctg gaggcgcggg cgcggctggg cggggcgacc
     5521 ttctcgttcc agcgggacgg gttgtggatc gacaacggcc agcacgtgtt cctgcgctgc
     5581 tgcacggcct accgctggct gctgcgccgg ctcggcgccg aggatctggt cacgctgcag
     5641 cggcggctgg cgctgccggt ggtcgcgccc ggcgggcggg tcggctggct gcggcgcggc
     5701 gccctgccgc tgccgccgcc gctgcacctg gccggggcgc tggcccgcta cccgtacctg
     5761 agctggccgc agcggctgcg ggtgggccgc gccgccctgg cgatgcggcg cctggacccg
     5821 gccgacgagg ccaacgaccg gcaggcgctg gcgccctggc tcgccgcgca cggccaggac
     5881 gccggcagcc tgcaggcgct ctgggagctg atctcgctgc cgaccctgaa cgcgcgggcg
     5941 gtggactcgt cgctggcgct cggcctcaag gtcttcaaga ccgggctgct ggaggccgcc
     6001 gactcggccg acatcggcta cgcgcgggtg ccgttgcagc agctccatgg cgagcgggcc
     6061 gagaaggccc tcggcgacgc gggcgcgcgg ctccggacgc actgccgggt gtcgaagatc
     6121 gagccgctcg gcggcccgga gggcgggttc gccgtcatgc ctggggaggg cgagcggctg
     6181 gaagcagcag ccgtgatcct cgcggtgccg cacgacggga ccggcgggct gctgcccacg
     6241 gcggcggaac ccgaccggct cggggccctt ggggcactcg gcagctcgcc gatcgtgaac
     6301 ctgcacgtcc gctacgaccg gcgggtgacc acgctcgact tcgccgccgg actccgcacg
     6361 ccggtgcagt gggtcttcga ccggaccgcc gccagcgggc tcgacccggc ctggggtgac
     6421 gggcagcacc tggcggtgtc gctgtcggcc gccg
//
LOCUS       DASWDC010000249         4170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_896355, whole genome shotgun sequence.
ACCESSION   DASWDC010000249 DASWDC010000000
VERSION     DASWDC010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896355"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /locus_tag="VF995_08170"
     CDS             <1..1779
                     /locus_tag="VF995_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX9343571.1"
                     /translation="EATYRARRGLDPERRLGCHARMVGDLVIDVPPDSQLHRQVVRKP
                     AEARAIELDPVVRLCYVEVERPSLGTQTSDHARLLQALEREWGLTGLTTDAQVVVGLQ
                     AALRTGDWAVTVAVHDGDAVTAVWPGFRDLALGIAFDVGSTTVAGHLCDLATGEVLAS
                     AGAMNPQIRFGEDLMSRVSYLMLNPGATAALTRAVRGCLNDLAATLAADAGRDPADVL
                     EVTLVGNPVMHHLLLGVDPTELGGAPFALATDQALRLRAASDLELAVHPGARAYVLPC
                     VAGHVGADTAGVLLAEAPYQRGAVSLLVDVGTNAEIVLGGHDRLLAASSPTGPAFEGA
                     QISAGQRAAPGAVERVRVDRDTLEPRLRVIGNPAWSDQPGFDPAAVSGICGSGIIEAV
                     AELYLAGVVTADGVIDGGAAARSPRVVRDGRTHAYVLHDRALQRQPILVTQNDIRQIQ
                     LAKAALHAGCRLLMDRLGVDSVDRVRLAGAFGAQIDPVHALVLGMVPDCDPAAVSSAG
                     NAAGTGARIALLSGRSRHEVEEAVRRVEKVETAVEPRFQEHFVDAMAIPHRRDPYARL
                     ARAVPLPERDPRARPRARTRTRRRTR"
     gene            1776..3320
                     /locus_tag="VF995_08175"
     CDS             1776..3320
                     /locus_tag="VF995_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019812318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX9343572.1"
                     /translation="MSDQAEPRGRRSGGREGRRAARLHAVAERVPFLTRTMAPFEVLS
                     EEGLALIEHNADVILEQVGVEFRDADALATLKDAGAEVDGELVRFPRGMARALVQATA
                     PREFTQHARNPANDVRIGGDATVFAPAYGPPFVRDLDQGRRYGTIEDFRNFVKLTYAS
                     PWLHHSGGTVCEPVDLPVNKRHLDMVEAHLRCSDKPFMGSVTHPDRARDTVEMARIVF
                     GAERLERDCVILSLINANSPLVWDATMLGAARVYAEANQATLMTPFILAGAMSPATVA
                     GTAAQTLAEALAGMAYVQLVRAGAPVVFGSFASSMSMQSGAPTFGTPEPALVLYTLAA
                     CARRLGVPFRSGGALTAAKVADAQAAYESANTMQPTMLSGVNFVLHVAGWLEGGLCMG
                     YEKFVLDNDQAGAWHAFAKGVDLSPNGQALDAIIANGPGQHFLGSAHTLANFETAFWR
                     SQLADANSFEQWELEGSRDATQRANALWKKTLADYQPPPIDDAVDQELREWIERRKAS
                     FPDSNV"
     gene            3425..3694
                     /locus_tag="VF995_08180"
     CDS             3425..3694
                     /locus_tag="VF995_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343573.1"
                     /translation="MGEQAIEQLARRTARLEDEFTNIHEALASIRSVQEDHSAQLADH
                     GVRLGRLEGGLDEVRGGVRTALEILERIERRTGGQANGGQTQAQN"
     gene            complement(3704..>4170)
                     /locus_tag="VF995_08185"
     CDS             complement(3704..>4170)
                     /locus_tag="VF995_08185"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEX9343574.1"
                     /translation="ALSQVSLRVDLTPAKGRPAPPLELPLQPNSWGRIGILEALWLGP
                     DEWLVTGGQGNAGKMVILLDAAFDGRPHSIVDVSANRVATDLTGADRLDLLATGCPLD
                     LHPRVWSEGACAQTLLARVPVLLQERASTTRLFVRPSFAAWLADWLAAAAPP"
BASE COUNT          604 a         1588 c         1438 g          540 t
ORIGIN      
        1 gaggcaacct accgggcgcg gcgcgggctc gacccggagc ggcggctcgg ctgccacgcg
       61 cgcatggtcg gcgacctggt gatcgacgtg ccgcccgaca gccagcttca ccggcaggtg
      121 gtgcgcaagc ccgccgaggc gcgcgcgatc gagctggacc cggtggtgcg gctctgctac
      181 gtcgaggtcg agcggccctc cctcggcacc cagaccagcg accacgcccg gctgctccaa
      241 gccctggagc gcgaatgggg cctgaccggc ctgaccaccg acgcgcaggt cgtcgtgggc
      301 ctgcaggcgg cgctgcggac cggcgactgg gccgtcaccg tcgccgtcca cgacggcgac
      361 gcggtcacgg cggtctggcc cgggttccgc gacctcgccc tcgggatcgc cttcgacgtc
      421 ggctcgacca ccgtcgccgg gcacctctgc gacctcgcca ccggcgaggt gctcgccagc
      481 gcgggcgcga tgaacccgca gatccgcttc ggcgaggacc tgatgagccg ggtcagctac
      541 ctgatgctca accccggcgc gaccgccgcg ctgacccgcg cggtgcgcgg ctgcctcaac
      601 gacctggccg ccaccctggc cgccgacgcg ggccgcgacc cggccgacgt gctggaggtc
      661 accctggtcg gcaacccggt catgcaccac ctgctgctcg gcgtcgaccc gaccgagctg
      721 ggcggcgcgc cgttcgcgct ggccaccgac caggcgctgc gcctgcgcgc cgcaagcgac
      781 ctggagctcg ccgtccaccc cggcgcgcgc gcctacgtgc tgccctgcgt cgccgggcac
      841 gtcggcgccg acaccgccgg ggtgctgctg gccgaggcgc cctaccagcg tggcgccgtg
      901 tcgctgctgg tggacgtcgg caccaacgcc gagatcgtgc tcggcggcca cgaccggctg
      961 ctggccgcct ccagccccac cggcccggcg ttcgagggcg cccagatctc cgccgggcag
     1021 cgtgccgccc ctggcgcggt cgagcgggtc cgggtcgacc gcgacaccct ggagccgcgt
     1081 ctccgcgtga tcggcaaccc cgcctggtcc gaccagccgg gtttcgaccc tgccgccgtc
     1141 agcgggatct gcggctccgg gatcatcgag gcggtcgccg agctgtacct cgccggggtg
     1201 gtcaccgcgg acggggtgat cgacggcggc gccgccgcgc gctccccccg ggtcgtccgg
     1261 gacgggcgca cccatgccta cgtgctgcac gaccgcgccc tccagcggca gccgatcctg
     1321 gtcacccaga acgacatccg ccagatccag ctcgccaagg cggccctgca cgcgggctgc
     1381 cggctgctga tggaccgcct cggcgtcgac agcgtcgacc gggtccgcct ggcgggcgcg
     1441 ttcggcgccc agatcgaccc ggtccacgcc ctcgtgctcg gcatggtccc cgactgcgac
     1501 ccggcggcgg tcagcagcgc cggcaacgcc gccgggaccg gcgcacggat cgcgctgctc
     1561 tccggccgct cccgccacga ggtcgaggag gccgtgcggc gcgtcgagaa ggtcgagacc
     1621 gccgtcgagc cgcgcttcca ggagcacttc gtcgacgcga tggcgatccc gcaccgccgc
     1681 gacccctacg cccgcctcgc ccgagcggtg ccgctcccgg agcgggaccc gcgggcgcga
     1741 cccagagcgc ggaccaggac cagaaggagg accaggtgag cgaccaggcc gagccgaggg
     1801 gacggcgcag cggcgggcgc gaggggcgcc gcgccgcgcg gctgcacgcg gtcgccgagc
     1861 gggtcccgtt cctcacccgc acgatggccc cgttcgaggt cctctccgag gagggcctcg
     1921 ccctgatcga gcacaacgcc gacgtgatcc tggagcaggt cggcgtcgag ttccgcgacg
     1981 ccgacgcgct cgccaccctc aaggacgccg gcgccgaggt ggacggcgag cttgtccgct
     2041 tccctcgcgg catggcccgc gcgctggtcc aggcgaccgc gccccgcgag ttcacccagc
     2101 acgcccgcaa ccccgccaac gacgtccgca tcggtggcga tgcaacggtg ttcgcccccg
     2161 cctacgggcc gccgttcgtc cgcgacctgg accagggccg ccgctacggg accatcgagg
     2221 acttccgcaa cttcgtgaag ctcacctacg cctcgccctg gctgcaccac agcggcggga
     2281 cggtctgcga gccggtcgac ctgccggtca acaagcgcca cctcgacatg gtcgaggcgc
     2341 acctgcgctg ctccgacaag ccgttcatgg gctcggtcac ccaccccgac cgcgcccgcg
     2401 acacggtcga gatggcccgg atcgtgttcg gcgccgagcg gctggaacgc gactgcgtga
     2461 tcctcagcct gatcaacgcg aactccccgc tggtctggga cgccaccatg ctcggcgccg
     2521 cccgcgtcta cgccgaggcc aaccaggcca ccctgatgac ccccttcatc ctggccggcg
     2581 cgatgagccc ggcgaccgtg gcgggcaccg ccgcccagac cctcgccgag gcactggccg
     2641 gcatggccta cgtgcagctg gtgcgggcgg gcgccccggt ggtgttcggc tcgttcgcct
     2701 cctcgatgtc gatgcagtcc ggcgccccca ccttcggcac ccccgagccc gcgctggtgc
     2761 tgtataccct ggccgcctgc gcgcgccggc tcggggtccc gttccgcagc ggcggcgcgc
     2821 tcaccgccgc caaggtcgcc gacgcgcagg ccgcctacga gtccgccaac accatgcagc
     2881 ccaccatgct cagcggggtg aacttcgtcc tgcacgtcgc cgggtggctg gagggcggcc
     2941 tctgcatggg ctacgagaag ttcgtgctcg acaacgacca ggccggcgcc tggcacgcct
     3001 tcgccaaggg cgtcgacctg tcccccaacg gccaggccct ggacgcgatc atcgccaacg
     3061 gccccggcca gcacttcctc ggctccgccc acaccctcgc caacttcgag accgccttct
     3121 ggcgctccca gctcgccgac gccaacagct tcgagcaatg ggaactggag ggctcccgcg
     3181 atgccaccca acgcgccaac gctctctgga aaaagaccct cgccgactac cagccccctc
     3241 caatcgacga cgccgtcgac caggagctgc gcgagtggat cgaacgccgc aaagcatcgt
     3301 tccccgactc gaatgtctga gtaggattat tctcctataa cctactcaag acttgaatgg
     3361 gacgcggagg gcctgcacgc attcggccaa tctgcgattc tggcaaacac gagcggagga
     3421 acgattgggc gaacaggcga tcgaacaact tgcgcggcgg acggcgcggc ttgaggacga
     3481 attcacgaac atccatgaag cccttgccag catccgctcg gtacaggaag accacagcgc
     3541 gcagctagct gaccacggcg tccgcctagg gcgccttgag ggcggcctcg acgaggtaag
     3601 aggcggggtc cggacggcgc tcgagatcct tgagcggatc gagcgcagga ccggcggcca
     3661 agccaacggc ggtcagacgc aagcgcagaa ctaggagctc acctcaaggg ggagcggcgg
     3721 cggcaagcca atcggccagc catgcggcga acgatgggcg tacgaacaat cgggtcgtgc
     3781 tggcgcgttc ctggagcagg acgggcacgc gggccagtag ggtctgcgcg caggcgccct
     3841 cgctccagac gcgggggtgc aggtcgaggg ggcagccggt cgccagcagg tcgaggcggt
     3901 ctgcgccggt caggtcggtg gcgacgcggt tggcgctgac gtcgactatg gagtggggcc
     3961 ttccgtcgaa ggcggcgtcc agcaggatca ccatctttcc ggcgttgcct tgaccgccag
     4021 ttaccagcca ttcgtcgggg ccgagccaca gggcctccag gatcccgatc cgtccccagc
     4081 tgttcggctg gagcgggagc tcgagcggtg gtgcgggccg acccttcgct ggcgtcaggt
     4141 ctacccgcag gctcacctgg gagagggctg
//
LOCUS       DASWDC010000250         3765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1399375, whole genome shotgun sequence.
ACCESSION   DASWDC010000250 DASWDC010000000
VERSION     DASWDC010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399375"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /gene="cysS"
                     /locus_tag="VF995_08190"
     CDS             <1..1264
                     /gene="cysS"
                     /locus_tag="VF995_08190"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX9343575.1"
                     /translation="LLRRALEAEGVEVRQVQNITDVGHLTDERFDRGEDRMLVSARLE
                     DKTPAEIAEHYTQRYLTDAAALNLEPAHALPRASAYVPRMIELVARLLEGGHAYQAGG
                     NVYFAVASFPAYGKLSRNTLDQLRAGHRQEWVDPHKRHHADFTLWKAAGPGRLVKWDS
                     PWGEGFPGWHIECSAMSLGELGERIEVHTGGVDNVFPHHEDEIAQSEAVVGHPVVGTW
                     VHGEHLLCEGRRMAKSAGNFYDLEEVRARGHAPLAVRYLFLQARYRTQLNFTWEALAG
                     AERALERLRARVAGWPRAPVQGAGDGNEGRARRYQERFQAAIGDDLDTPRALAILGEL
                     EADEALAPATRLSLVQRFDRVLGLDLDRSARSGQTLPPGAAELIERREAARSGRDWAT
                     ADRLREELAALGVEVADTGSGTTWRLRT"
     gene            1350..2171
                     /locus_tag="VF995_08195"
     CDS             1350..2171
                     /locus_tag="VF995_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX9343576.1"
                     /translation="MGLMKRFSMIFKAKASKALDRYEDPRETLDYSYERQLEMLQKMR
                     RGVADVATSRKRIELQAQQLQQSAAKLEGQAKQAVSANREDLAREALTRRAAIAGQLS
                     DLQAQHDQLKGEEAKLVDASQRLQTKIEAFRTRKETIKATYTAAEAQTKVNEAISGIS
                     EEMGDVGMAMQRAEDKVSIMRSRAGALDELMASGALDDASMANDRLQAELDRSLATPG
                     IEAELARIRGEIGGASTTAKQLGAPAEHAPGASVPSESETPAEAPVQAPAQEEQR"
     gene            2168..2446
                     /locus_tag="VF995_08200"
     CDS             2168..2446
                     /locus_tag="VF995_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343577.1"
                     /translation="MIIRVVGEGQFEVPDGHLEALNRLDEDLLKACDSGDEARFATAL
                     DALITSVRSAGSELPADYLGASDLVLPGPDSTIEEVRNLLGDEGLIPG"
     gene            2460..3389
                     /gene="htpX"
                     /locus_tag="VF995_08205"
     CDS             2460..3389
                     /gene="htpX"
                     /locus_tag="VF995_08205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019360016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX9343578.1"
                     /translation="MATHSRYPADRGLAARMLLTMFLIGLIYVAFIAVLIAIRIPVVL
                     VVLIAGGFLFFQYFYSDKIALFSMRAREVSPQEAPELHAIVDRLCALADMPKPRVAIA
                     DNDMPNAFATGRNQKNAVVCATTGIMRRLEPPELEAVLAHELSHVAHRDVAVMTIASF
                     LGLLAGFVVRSAIYAGAWGGFGGGYGRRDDRDNTGAIILLVVLASAIVYALSFLLTRA
                     LSRYRELSADRSGAILIGRPSLLATALTKVTGQMGRIPTRDLRAAEPLNAFFFAPALS
                     AGFSFSSLFSTHPSLERRLEQLAKLEAEMGRYA"
     gene            3386..>3765
                     /locus_tag="VF995_08210"
     CDS             3386..>3765
                     /locus_tag="VF995_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343579.1"
                     /translation="MKLLDTLLGRSKPKQANLDALFAVSGAAITLEAGAGLKPSGEAA
                     VCFKPASGEGFTATSTELTELLDLAVKEAGSTLRVEDDRYGYRWVIIGDPDLEDLVTS
                     VHLINTTLKEHGYGPQLLCTVFGFT"
BASE COUNT          577 a         1376 c         1308 g          504 t
ORIGIN      
        1 cctgctgcgg cgggcgctgg aggccgaggg tgtcgaagtc cggcaggtgc agaacatcac
       61 cgacgtcggg cacctcaccg acgagcggtt cgaccggggc gaggaccgca tgctggtctc
      121 ggcccgcctg gaggacaaga cgcccgccga gatcgccgag cactacaccc agcgctacct
      181 gaccgacgcg gcggcgctga acctggagcc ggcccacgcc ctcccgcgcg cctccgcgta
      241 cgtcccccgg atgatcgagc tggtcgcgcg cctgctggag ggcggccacg cctaccaggc
      301 gggcggcaac gtctacttcg cggtggcgtc gttcccggcc tacgggaagc tgtcgcgcaa
      361 caccctcgac cagctccgcg ccggccaccg gcaggagtgg gtggaccccc acaagcgcca
      421 ccacgcggac ttcaccctct ggaaggcggc cgggccggga cggctggtca aatgggactc
      481 gccctggggc gaggggttcc ccggctggca catcgagtgc tcggcgatgt cgctcggcga
      541 gttgggcgag cggatcgagg tgcacaccgg cggggtcgac aacgtcttcc cccaccacga
      601 ggacgagatc gcccagtccg aggcggtcgt cggccacccg gtggtcggca cctgggtcca
      661 cggcgagcac ctgctctgcg agggccgccg catggccaag tcggcgggca acttctacga
      721 cctggaggag gtccgcgcgc gcggccacgc cccgctggcg gtccgctacc tgttcctgca
      781 ggctcgctac cgcacccagc tgaacttcac ctgggaggcg ctggcagggg ccgagcgggc
      841 cctggagcgc ctgcgcgccc gggtcgccgg gtggccccgc gctccggtgc agggcgcggg
      901 tgacggcaac gagggacgcg cccgccgtta ccaggagcgc ttccaggcgg cgatcggcga
      961 cgacctggac acgccgcggg cgctggcgat cctcggcgag ctggaggccg acgaggcgct
     1021 cgcccccgcc acgcggcttt ccctggtcca gcggttcgac cgggtgctcg ggctggacct
     1081 ggaccgctcg gccaggtccg gccagaccct gcccccggga gcggccgagc tgatcgaacg
     1141 ccgggaggcg gcccgctccg gccgcgactg ggccaccgcg gaccgcctgc gcgaggagct
     1201 ggccgctctg ggcgtcgagg tggccgacac cggttcggga accacgtggc ggctgaggac
     1261 ctgaccctgt ggacaaccct cggcctccac ccgcagcaag tcgtatgctg ctaggagcat
     1321 gtgagcgacg gccgtcagga gtgtgagcga tgggcttgat gaagcgattt tcgatgatct
     1381 tcaaggcgaa ggcatcgaaa gcgctcgacc ggtatgagga ccccagggag acactggact
     1441 actcgtacga gcgccagctg gagatgctgc agaagatgcg gcggggcgtc gccgacgtgg
     1501 ccacctcccg caagcgcatc gaactgcagg cccagcagct gcagcagtcg gcggccaagc
     1561 ttgagggcca ggccaagcag gccgtctccg ccaaccgcga ggacctggcc cgggaggcgc
     1621 tgacccgccg ggccgcgatc gccggccagc tctccgacct gcaggcccag cacgaccagc
     1681 tcaagggcga ggaggccaag ctggtggacg cctcccagcg cctgcagacc aagatcgagg
     1741 cgttccggac ccgcaaggaa accatcaagg cgacctacac cgccgccgag gcgcagacca
     1801 aggtcaacga ggccatctcg ggcatcagcg aagagatggg cgacgtcggc atggcgatgc
     1861 agcgcgccga ggacaaggtc tcgatcatgc ggtcgcgcgc cggcgccctg gacgagctga
     1921 tggcctcggg cgccctcgac gacgcctcga tggccaacga ccgcctccag gcggagctgg
     1981 accgctccct ggccaccccg gggatcgagg cggagctcgc caggatcagg ggcgagatcg
     2041 gcggcgccag caccaccgcc aagcagctcg gcgcccccgc cgagcacgca ccgggcgcct
     2101 cggtgccgtc cgagagcgag accccggccg aggctcccgt ccaggcacca gcccaggagg
     2161 agcagcgatg atcatccggg tcgtgggcga ggggcagttc gaggtacccg acggccacct
     2221 cgaagcgctg aaccgcctcg acgaggacct gctgaaggcg tgcgactccg gggacgaggc
     2281 ccgcttcgcc accgcgctgg acgcgctgat caccagcgtg cgcagcgccg gcagcgagct
     2341 gcccgccgac tacctcggcg cctccgacct ggtcctgccg gggcccgact ccaccatcga
     2401 agaggtccgc aacctcctcg gcgacgaagg gctcatccca gggtgaaggt gagacgctga
     2461 tggccaccca ctcgcgctac cccgccgacc ggggactggc agcccgcatg ctgctgacca
     2521 tgttcctcat cggcctgatc tatgtggcgt tcatcgccgt gctgatcgcg atccgcatcc
     2581 cggtggtgct ggtcgtgctg atcgccggcg gtttcctgtt cttccagtac ttctactccg
     2641 acaagatcgc cctgttctcg atgcgtgccc gcgaggtctc cccgcaggag gcgccggagc
     2701 tgcacgccat cgtcgaccgg ctctgcgccc tggccgacat gcccaagccg cgggtggcga
     2761 tcgccgacaa cgacatgccc aacgccttcg ccaccggccg caaccagaag aacgcggtgg
     2821 tctgcgccac caccgggatc atgcggcggc tggagccgcc ggagctggag gcggtgctgg
     2881 cgcatgagct gtcccacgtc gcccaccgcg acgtggccgt gatgaccatc gcgagcttcc
     2941 tcggcctgct cgccgggttc gtggtccgct cggccatcta cgccggcgcc tggggcgggt
     3001 tcggcggcgg ctacggccgg cgcgacgacc gcgacaacac cggcgcgatc atcctgctgg
     3061 tggtgctcgc ctcggcgatc gtctacgcgc tcagcttcct gctgacgcgg gcgctgtccc
     3121 gctaccggga gctgtcggcg gaccgctccg gcgcgatcct gatcggccgc ccgtcgctgc
     3181 tggcgaccgc gctcaccaag gtcaccggcc agatgggccg catccccacc cgcgacctgc
     3241 gcgccgccga gccgctcaac gccttcttct tcgcgccggc gctgtcggcc gggttcagct
     3301 tctcgtcgct gttctcgacc cacccgagcc tcgagcgccg gctggagcag ctcgccaagc
     3361 tggaggcgga gatgggccgg tacgcgtgaa gctcctcgac acgctgctcg gccggtccaa
     3421 acccaagcag gccaacctcg acgcgctgtt cgcggtcagc ggcgccgcca tcaccttgga
     3481 ggctggtgcc ggcctgaagc cgtccggcga ggcagcggtc tgcttcaagc cggccagcgg
     3541 cgaggggttc accgccacct cgaccgagct gaccgagctg ctcgacctgg cggtcaagga
     3601 agccggctcg accctgcggg tggaggacga ccgctacggc taccgctggg tgatcatcgg
     3661 cgaccccgac ctggaggacc tggtcaccag cgtccatctc atcaacacca ccctgaagga
     3721 gcacggctac ggcccgcagc tgctctgcac ggtgttcggc ttcac
//
LOCUS       DASWDC010000251         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_566870, whole genome shotgun sequence.
ACCESSION   DASWDC010000251 DASWDC010000000
VERSION     DASWDC010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566870"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VF995_08215"
     CDS             <1..990
                     /locus_tag="VF995_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343580.1"
                     /translation="RHAGPLAPVISALLAKSPEARPSGARLRGLLQVAEAGVQAGVAT
                     EPQPATQPLPPPSMGWPTRRRADRRPLWIVGGTLLALLVALVVALWQSGVLTGPETKP
                     PARGGTASGASGAGSNAAQGQRSAPTTTQPGPSASSQPQGSDSGAGSAAATGNTQAAT
                     ATPVTLPTSEQPAPGQLLPKFWTSATNQAGGYSVGVPQTWDVSATGTTTDVEWLGERT
                     FEAAFQVQALPQTQAPLQWLKGQAATFATDHQSDSYTQLKLTSHWNYKGQPAAAWEFT
                     WFRNGEKTHARWVAFRSGSHTFAVLYRSRDVFWLGGGSAVFPEGFEQAFTPLG"
     gene            complement(1000..1491)
                     /locus_tag="VF995_08220"
     CDS             complement(1000..1491)
                     /locus_tag="VF995_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX9343581.1"
                     /translation="MSVTDELLQNNQAYAASFDKGALPLPPAKPVVVVACMDARLIPT
                     RVLGLNEGDAHVIRNAGGAVTDDVIRSLTISQRLLGTREVILVHHTDCGMLTFADDDL
                     KRQVQADTGVRPPFAFEAFPDLEEDVRQSVARVKASPFVPHTDQVRGFVYEVESGRLR
                     VVS"
     gene            complement(1556..2365)
                     /locus_tag="VF995_08225"
     CDS             complement(1556..2365)
                     /locus_tag="VF995_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX9343582.1"
                     /translation="MSSYDPASTAGVPVHGRERLAQMKRGLFTSDLSVNEFLLVKHAG
                     FEPLGLVVGSSIYHIGYQQSAWKQSMEMAVLSQAMYEARELAMTRMEEEADQLGADGV
                     VGVRLDIGRYEWGQHMAEFIAIGTAVRHRDGELHRAPNGRPFTSDLSGQDFWTLLASG
                     HRPVGMVMGSCVYHVAHQGMFASMRQVGQNVELPNFTQALYDARELAMERMQREAEEV
                     QAEGVVGVQLQERSHGWGSHVIEFFAVGTAIVPLSGEHQLPPPTLTLSLTD"
     gene            complement(2358..3443)
                     /locus_tag="VF995_08230"
     CDS             complement(2358..3443)
                     /locus_tag="VF995_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX9343583.1"
                     /translation="MPLFRRGGAGGAAPPDREARQRAEAEQAASREALERGGLPLKAQ
                     QRLAALGSSGGGGFSFTSDLSVNEFALGAAIGLRPLSQVMGSSVYHVGWQQMPGSWGS
                     GWGAGWQPDGISQELGVLSDAWNTARRLAFGRLEQEARLVGAHAVVGVHLTRRQHEWA
                     SGSIEYIAVGTAVRIEGAPPAEQPALTDLSGQDFWKLWQAGYAPLGVVGASTVFYVMP
                     SWSTQWAQSGGIFGSGWNNQELGDFTQALYEAREVALGRVTAEARALGAAGVVGVSID
                     QSEREREVEQNNRKRTDLIITFHVLGTAIGAQRDHAADLTVTPTLALRAGRKERLAAG
                     LPQELAQQLANLPPNVAALLRQESNNE"
     gene            complement(3454..3855)
                     /locus_tag="VF995_08235"
     CDS             complement(3454..3855)
                     /locus_tag="VF995_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343584.1"
                     /translation="MDEGMAFELLAASLRASSSDLETFVKVLAGKLEQALPGRAQVER
                     RPARFLSKQTRVERIECQLGDRRYLLAARRGAVETRCATVVRGIVLKSAQLPLDQWIE
                     ALARDLASEAQRSEASRLALERLLSQDQTQE"
BASE COUNT          544 a         1425 c         1430 g          582 t
ORIGIN      
        1 aggcacgccg gccccctcgc cccggtgatc tctgccctgc tggccaagtc accggaggcc
       61 cgcccgtccg gcgcgcgcct gcgcgggctg ctgcaggtgg ccgaagccgg ggtccaggcc
      121 ggggtcgcga ccgagccgca gccagccact cagccgctgc ctccgccgtc gatggggtgg
      181 cccacccggc ggcgggcaga tcgaaggccg ttgtggattg tcgggggcac gctcctcgcc
      241 ttgctggtcg cgctcgtcgt cgcgctgtgg cagagcgggg tcttgaccgg tccggagacc
      301 aagcctccgg ctcgcggcgg cacggcttcc ggtgcatccg gcgcaggctc caacgccgcg
      361 caggggcagc ggtccgcgcc gacgacgacg cagccgggac catcggccag cagccagccg
      421 caaggctccg actccggcgc tggctctgcc gctgccaccg gcaacacgca ggccgcgacg
      481 gcgacgccgg tgaccctgcc gaccagcgag cagccagcgc cagggcagct cctgccgaag
      541 ttctggacct cggcgaccaa ccaggcgggc ggctacagcg tcggcgtgcc gcagacctgg
      601 gacgtgagcg ccaccggcac caccaccgac gtggagtggc tcggcgagcg gaccttcgag
      661 gcggccttcc aggtccaggc gctcccccag acccaggccc cgctccagtg gctgaaggga
      721 caggccgcca cgttcgccac cgaccaccag tcggacagct acacccagct caagctgacc
      781 agccactgga actacaaggg tcagccggcg gccgcctggg agttcacctg gttccgcaat
      841 ggggagaaga cccacgcgcg ctgggtcgcc ttccgctccg ggagccacac cttcgcggtc
      901 ctctaccgca gcagggacgt gttctggctg ggcggcggct cggcggtgtt cccggaaggc
      961 ttcgagcagg cgttcacgcc gctcgggtga gggcccggct cagctgacta cgcgcaggcg
     1021 gccgctctcc acctcataga cgaacccgcg gacctggtcg gtgtggggca cgaacgggct
     1081 tgccttgacg cgggcgaccg actggcgaac gtcctcctcc aggtcgggga aggcctcgaa
     1141 ggcgaacggc ggccggacgc cggtgtcggc ctggacctgg cgcttcaggt cgtcgtcggc
     1201 gaaggtgagc atgccgcagt cggtatggtg gacgaggatg acctcgcggg tgccgagcag
     1261 gcgctgggag atcgtcagcg agcggatcac gtcgtcggtg accgcgccgc cggcgttgcg
     1321 gatcacgtgc gcgtcgccct cgttcagtcc cagtacccgg gtcgggatca gccgcgcgtc
     1381 catgcacgcc accaccacga ccggcttggc cggcggcagt ggcagggccc ccttgtcgaa
     1441 gctcgccgcg taggcctggt tgttctggag cagctcgtcg gtcacgctca tggcacgcct
     1501 cctgggccgg gtccgtccgg atttgcgagt gtgccctacc ttactcggac gccggtcagt
     1561 cggtcaggct cagcgtcagg gtcggcggcg gcagctggtg ctccccgctg agcgggacga
     1621 tcgcggtgcc gacggcgaag aactcgatga cgtgggagcc ccagccgtgg gagcgttcct
     1681 ggagctggac gccgacgacg ccctcggcct ggacctcctc ggcctcgcgc tgcatccgct
     1741 ccatggccag ctcgcgggcg tcgtagaggg cctgggtgaa gttgggcagc tccacgttct
     1801 ggccgacctg gcgcatcgag gcgaacatcc cctggtgggc gacgtggtag acgcaggagc
     1861 ccatcaccat cccgaccggg cggtgcccgg aggcgagcag cgtccagaag tcctggccgg
     1921 agaggtcgct ggtgaagggg cggccgttgg gggcgcggtg cagctcgccg tcgcggtggc
     1981 ggaccgccgt gccgatggcg atgaactcgg ccatgtgctg gccccactcg tagcggccga
     2041 tgtcgaggcg aaccccgacg acgccgtcgg cgccgagctg gtcggcttcc tcctccatgc
     2101 gggtcatggc cagctccctg gcctcgtaca tggcctgcga caggacggcc atctccatcg
     2161 actgcttcca ggccgactgc tggtagccga tgtggtagat cgagctgccc accaccagcc
     2221 cgagcggctc gaagccggca tgcttgacca gcaggaactc gttgaccgac aggtcggagg
     2281 tgaacaggcc gcgcttcatc tgcgcgaggc gctcgcgccc gtggacgggc acccccgcgg
     2341 tcgaggccgg gtcgtagcta ctcattgttg gactcctgtc gtagcagcgc ggccacgttt
     2401 ggtgggaggt tggccagctg ttgtgccagt tcctgcggca gcccggcggc gagccgctcc
     2461 ttcctgccgg cgcgcagcgc gagcgtgggc gtcacggtca ggtcggccgc gtggtcgcgc
     2521 tgcgcgccga tggcggtgcc gagcacgtgg aaggtgatga tcaggtcggt gcgcttgcgg
     2581 ttgttctgct cgacttcgcg ttcgcgctcg ctctggtcga tcgagacgcc gacgacccct
     2641 gccgcgccga gggccctcgc ctcggcggtc acccggccga gcgcgacctc gcgggcctcg
     2701 tagagggcct gggtgaagtc accgagctcc tggttgttcc agcccgagcc gaagatgccg
     2761 cccgactggg cccactgcgt cgaccagctc ggcatgacgt agaagacggt ggaggcgccg
     2821 accacgccga gcggcgcgta gcccgcctgc cacagcttcc agaagtcctg gccggagagg
     2881 tcggtgagcg ccggctgttc cgctggaggc gccccctcga tccgcaccgc ggtgccgacc
     2941 gcgatgtact cgatcgagcc ggacgcccat tcgtgctggc ggcgggtcag gtggacgccg
     3001 acgaccgcgt gggcgccgac cagccgggcc tcctgttcga ggcgaccgaa ggcgagccgc
     3061 cgggcggtgt tccaggcgtc ggagaggacg cccagctcct gcgagatccc gtcgggctgc
     3121 cagccggccc cccagccgct gccccagctg ccgggcatct gctgccagcc gacgtggtag
     3181 accgagctgc ccatcacctg cgagaggggc cgcaacccga tcgccgcgcc gagggcgaac
     3241 tcgttgaccg acaggtcgga ggtgaagctg aagccaccgc cgcccgagga gcccagcgcc
     3301 gccaggcgct gctgggcctt cagcgggagg ccgccgcgtt ccagcgcctc gcgggaggcg
     3361 gcctgctccg cctcggctcg ctggcgcgcc tcacggtcgg gcggcgcggc gccaccggcg
     3421 cctcctcgac ggaacagcgg catgggcctc cctctattcc tgggtctggt cctggctgag
     3481 gaggcgctcc agcgcgagcc tcgacgcctc gctccgctgc gcctcgctgg ccaggtcgcg
     3541 ggcgagcgcc tcgatccact ggtccagcgg gagctgcgcg ctcttgagca cgatcccacg
     3601 gaccaccgtg gcacagcggg tctcgaccgc gccgcgacga gcggccagca ggtagcgccg
     3661 gtcgccgagc tggcattcga tccgctcgac ccgcgtctgc ttggagagga accgcgccgg
     3721 gcggcgctcc acctgggccc ggccgggcag cgcctgctcc agcttgccgg cgaggacctt
     3781 cacgaacgtc tccaggtcac tgctggaggc acgcaacgag gccgcgagca gctcgaaggc
     3841 catgccctcg tccaagcgtc gatcctcccc cgattcgctg ttggcgacct cgctacctgg
     3901 cgggaaagtc tacttcagcg ccgccatttg cgtatgggcg ctccatacgg acgcatccgc
     3961 tggcgacttg tgatgtggtc g
//
LOCUS       DASWDC010000252         4628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1319606, whole genome shotgun sequence.
ACCESSION   DASWDC010000252 DASWDC010000000
VERSION     DASWDC010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319606"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="VF995_08240"
     CDS             complement(<1..1632)
                     /locus_tag="VF995_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX9343585.1"
                     /translation="MQVRSGFISDLWWKNAVIYGVDVKCFQDSDDDGIGDFPGLTERL
                     EYLASLGVTCLWLLPIMPSPWRDNGYDVTDYYNVHPRLGTLGDVVDCLRTARELGLRV
                     ITDLVVNHTSDQHPWFQQARSSRDNPFRDWYVWRDRPDKEPEGVSFPGEEDSNWCFDE
                     GTGQWYLHRFYHFQPDLNIENPKVRREINKMMGFWLQLGFSGFRIDAAPFLIETAGIP
                     GKTPDHDPQAPLRDMRGFLSRRRGDAILLGEANVAPDRLTDYFGERGDELHLLLNFVL
                     NQGLYLSLAREDPGPLRDHLEELPKVPRIGQFVNFVRNADELTLDKLSKPEQEEVFRR
                     FAPEASMRLYGHGIRRRLPPMVDGDRRRIELAYSLLFSLPDTPLLLYGEEIGLGDDLS
                     TPGRGSVRVAMQWTDRPGGGFSDADPGEFLPPLVSEGPFGSRQVNVAAQRRDPRSLLN
                     RVARMIHLRREAPEIGFGRWRLLDTDQPGVLAHRCDWEGGTFVALHNFTGNAVQVKLD
                     LGDDDVEGVGAGRVEELFADQAEEHLGTVPPEPPGA"
     gene            complement(1723..2433)
                     /locus_tag="VF995_08245"
     CDS             complement(1723..2433)
                     /locus_tag="VF995_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343586.1"
                     /translation="MPARPQPGKPSTHPRIRWLAILALLALAAMLLAPVGASASTSAA
                     PCPTSGGIPFSSTLTNGTVTIGKHAHASGTSGSACGLVTFDPNIGLVSTIAQDNITFN
                     PFTLHLGLLSIPTQVTAVSDFHGAVAGNPDGTTSITLTGSVTATSKILGFSCGIGPFT
                     PTLTTGTSGSLSGTPLVGQLPGPLTGKLVANDFAVPKATASRSCPKLIAALVNLTVGL
                     PQPAGGSSLTSDVSLSTS"
     gene            complement(2530..2967)
                     /gene="arfB"
                     /locus_tag="VF995_08250"
     CDS             complement(2530..2967)
                     /gene="arfB"
                     /locus_tag="VF995_08250"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX9343587.1"
                     /translation="MAEVVRITPEVTVPVDELEFRVSRSSGPGGQGVNTTDSRVELRF
                     DLAGSPSLPPAARERALRRLASRVDSTGTLRVVAQAQRSQLANRRAALERFAELLGDA
                     LATPKHRTPTKPTAASRRRRLQAKRRRASTKRTRHPPNSLDED"
     gene            complement(2983..>4628)
                     /locus_tag="VF995_08255"
     CDS             complement(2983..>4628)
                     /locus_tag="VF995_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017564728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX9343588.1"
                     /translation="PAGRARRVRRSYRGDTLVLETEFRTSEGTVRLVDCMPPRGQAPD
                     VVRVVEGIHGRVPMRMDLRIRFDYGSIVPWVRRREDALHAIGGPDSVWLYTPVELRGE
                     DLSTVAEFTVAEGEQVPFTLTWQASHLPRPRRIDPFRAVDDTEAWWAHWAGRLAYEGG
                     WRDAVVRSLLTLKALTYHPTGGIVAAPTTSLPEQLGGVRNWDYRYCWLRDATFALYAL
                     MSCGLVGEAKAWREWLLRAVAGAPKQMQILYGVAGERRILENELEWLPGYEGARPVRV
                     GNAAVDQFQLDVYGEVMDTLHLGRMAGIEHEDAAWDFQRKLVEFVEDCWRKPDEGLWE
                     IRGPRRDFTHSKVMAWVALDRAVKAVELFGRPGPLQRWRALRHEIHEEVLERGYDADR
                     KSFVQYYGAKQLDASLLMIPLVGFLPATDPRMRGTVDAIQRELRQDGFVLRYPTDAAE
                     PVDGLPPGEGAFLACSFWLADNLALMHRHDEAREQFERLLELRNDVGLLAEEYDPKAG
                     RQVGNFPQAFSHVPLINTAYHLSGLGPGGKGPAWARHRA"
BASE COUNT          651 a         1573 c         1734 g          670 t
ORIGIN      
        1 cgcgccgggg ggttcggggg gcacggtccc gagatgctcc tcggcctggt cggcgaacag
       61 ctcctcgacc cgcccggcac ccacgccctc cacgtcgtcg tcgccgaggt cgagcttcac
      121 ctggacggcg ttgccggtga agttgtgcag cgcgacgaag gtgccgccct cccagtcgca
      181 gcgatgcgcg agcacgccgg gctggtcggt gtcgagcagc cgccagcggc cgaaaccgat
      241 ctccggcgcc tcgcgccgca ggtggatcat gcgcgcgacc cggttcagca gcgaacgcgg
      301 gtcgcgccgc tgggcagcca cgttgacctg ccgggagccg aacggcccct cgctgaccag
      361 cggcggcagg aactcgccag ggtcggcgtc ggagaacccg ccgcccggcc ggtcggtcca
      421 ctgcatcgcc acccgcacgc tgccccggcc gggcgtcgac aggtcgtcgc cgaggccgat
      481 ctcctcgccg tagagcagca gcggggtgtc cggcaggctg aacagcaggc tgtaggccag
      541 ctcgatgcgg cgccggtcgc cgtccaccat cggcggcagg cggcggcgga tgccatggcc
      601 gtagagccgc atcgacgcct cgggcgcgaa gcggcggaag acctcctcct gctccggttt
      661 ggagagcttg tcgagggtca gctcgtcggc gttacggacg aaattgacga actggccgat
      721 gcgcgggacc ttcggcagct cctccagatg gtcgcgcagc gggccggggt cctcccgcgc
      781 cagcgacagg tacaggccct ggttgagcac gaagttcagc agcaggtgca gttcgtcgcc
      841 gcgctccccg aagtagtcgg tgagccggtc gggcgcgacg ttggcctcgc cgagcaggat
      901 cgcgtcgccg cggcggcgcg acaggaagcc gcgcatgtcc cgcagcggcg cctgcgggtc
      961 atggtcgggg gtcttgccgg ggatccccgc ggtctcgatc aggaacggcg ccgcgtcgat
     1021 ccggaagccg gagaagccga gctgcagcca gaagcccatc atcttgttga tctcccggcg
     1081 gaccttgggg ttctcaatgt tgaggtccgg ctggaagtgg tagaagcgat gcaggtacca
     1141 ctggccggtg ccttcatcga agcaccagtt ggagtcctcc tcgccgggga agctgacccc
     1201 ctccggctcc ttgtcgggcc ggtcgcgcca gacataccag tcgcggaacg ggttgtcgcg
     1261 gctcgaccgg gcctgctgga accaggggtg ctggtcggag gtgtggttga cgaccaggtc
     1321 ggtgatcacc cgcaggccca gctcccgcgc ggtgcgcagg cagtcgacca cgtcgccgag
     1381 agtgccgagg cgggggtgga cgttgtagta gtcggtcacg tcgtagccgt tgtccctcca
     1441 gggggagggc atgatcggca gcagccacag gcaggtcacg cccaggctcg ccaggtactc
     1501 cagccgctcg gtcaggccgg ggaagtcgcc gatcccgtcg tcgtcggagt cctggaagca
     1561 cttgacgtcc acgccgtaga tgacggcgtt cttccaccac aagtcgctga taaagccgga
     1621 tcgcacctgc atgaaccaag cttccccccc ggacccggtg gtgaatcggc cgggaacggg
     1681 ggggaagcgg ccggggggcc cggctgtgcg gctgcggtca gctcagctgg tggacaggct
     1741 cacgtcggag gtgagcgagg agcctcctgc tggctgcggc aggccgacgg tgaggttgac
     1801 gagcgcggcg atcagcttgg ggcaggaccg cgacgcggtc gccttgggga cggcgaagtc
     1861 gttggcgacc agcttgccgg tgagcgggcc ggggagctgg ccgaccagcg gcgtgccgct
     1921 gagcgagccg ctggtgccgg tggtcagggt gggcgtgaac ggcccgatgc cgcagctgaa
     1981 cccgaggatc ttcgaggtgg cggtcaccga gccggtcagg gtgatgctgg tggtcccgtc
     2041 cgggttgccg gcgacggcgc cgtggaagtc gctgaccgcg gtcacctgcg tcgggatcga
     2101 cagcagcccg aggtgcaggg tgaaggggtt gaaggtgatg ttgtcctggg cgatggtcga
     2161 gaccagcccg atgttggggt cgaaggtgac cagcccgcag gcgctgccgc tggtcccgct
     2221 ggcgtgggcg tgcttgccga tggtcacggt gccgtttgtg agcgtgctgg agaacgggat
     2281 cccgccgctg gtcgggcagg gcgcggcgct ggtggaggcc gaggcgccca ccggcgccag
     2341 cagcatcgcg gccagcgcga gcagcgccag aatggccagc cagcggatgc gggggtgtgt
     2401 ggagggcttg cctggttgtg gtcgtgcggg catgcgcacc aacctcctca ggtggcgtga
     2461 tctttagtta ctgatcagta acaaccaagc aagcgaacaa tacggacgtt tcacgggatc
     2521 tgtcaaccct tagtcctcgt ccagcgagtt gggggggtgg cgggtccgct tggtggaggc
     2581 gcggcggcgt ttggcctgca ggcggcggcg gcgggaggcg gcggtcggtt tggtcggagt
     2641 gcggtgcttg ggagtggcca gggcgtcgcc cagcagctcg gcgaagcgct ccagcgccgc
     2701 gcggcggttg gcgagctggc tgcgctgcgc ctgcgccacc acccgcagcg tcccggtgga
     2761 gtcgactcgg gaggcgaggc ggcgcagcgc ccgctccctg gcggcgggcg gcagcgacgg
     2821 cgagccggcc aggtcgaagc gcagctccac ccgcgaatcg gtggtgttga cgccctgtcc
     2881 gcccggcccg gaggagcggc tcacccggaa ctccagctcg tcgacgggga ccgtcacctc
     2941 gggagtgatc cggacgacct ccgccatgcg tgcggccccc gctcaggccc ggtggcgggc
     3001 ccaggcggga cccttcccgc ccggccccag gcctgacaga tggtaggcgg tgttgatcag
     3061 cggcacgtgc gagaacgcct gcgggaagtt gccgacctgg cgcccggcct tcgggtcgta
     3121 ctcctcggcg agcaggccga cgtcgttgcg cagttccagc agccgctcga actgctccct
     3181 ggcctcgtcg tggcggtgca tcagcgcgag gttgtcggcc agccagaagc tgcacgccag
     3241 gaaggcgccc tcgccgggcg gcaggccgtc gaccggctcg gcggcgtcgg tcgggtagcg
     3301 cagcacgaag ccgtcctggc gcagctcccg ctggatcgcg tcgaccgtgc cgcgcatgcg
     3361 cgggtcggtg gctggcagga agcccaccag cgggatcatc agcaggctgg cgtcgagctg
     3421 cttggcgccg tagtactgca cgaaggactt gcggtcggcg tcgtagccgc gttcgaggac
     3481 ctcttcgtgg atctcgtgcc gcaacgcccg ccagcgctgc agcgggccag ggcggccgaa
     3541 cagctcgacc gccttgaccg cgcggtcgag cgccacccag gccatcacct tggagtgggt
     3601 gaagtcccgc cgcggcccgc ggatctccca caggccctcg tccggcttgc gccagcagtc
     3661 ctcgacgaac tcgaccagct tgcgctggaa gtcccaggcg gcgtcctcgt gctcgatgcc
     3721 ggccatgcgg ccaagatgca gggtgtccat cacctcgccg tagacgtcca gctggaactg
     3781 gtccacggcg gcgttgccga cccggaccgg ccgggcgccc tcatagccgg gcagccactc
     3841 cagctcgttc tccaggatgc gccgctcacc ggcgacgccg tagaggatct gcatctgctt
     3901 gggcgccccg gcgaccgcgc gcagcagcca ctcccgccac gccttggcct cgccgaccag
     3961 cccgcagctc atcagcgcgt agagcgcgaa ggtcgcgtcg cgcagccagc agtagcggta
     4021 gtcccagttg cgcacccccc cgagctgctc gggcagcgag gtggtgggcg cggcgacgat
     4081 cccgccggtc gggtggtagg tgagcgcctt gagggtgagc agcgagcgga ccaccgcgtc
     4141 gcgccagccg ccctcgtagg cgagccggcc ggcccagtgc gcccaccacg cctcggtgtc
     4201 gtcgaccgcc cggaacggat cgatcctgcg gggtcgcggc aggtgcgacg cctgccaggt
     4261 gagcgtgaac ggcacctgct cgccttcggc cacggtgaac tccgccacgg tggagaggtc
     4321 ctcgccgcgg agctcgaccg gcgtgtacag ccacaccgag tcgggcccgc cgatggcgtg
     4381 cagggcgtcc tcccggcggc gcacccacgg gacgatcgag ccgtagtcga agcggatccg
     4441 caggtccatc cgcatcggca cgcgcccgtg gatgccctcc acgacgcgga cgacgtccgg
     4501 ggcctgcccg cgcggcggca tgcagtcgac cagccgcacg gtcccctcgc tggtgcggaa
     4561 ttccgtctcc agcaccagcg tgtcgccccg gtagctgcgc cggacgcggc gcgcccgccc
     4621 ggccggcg
//
LOCUS       DASWDC010000253         3907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1319641, whole genome shotgun sequence.
ACCESSION   DASWDC010000253 DASWDC010000000
VERSION     DASWDC010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319641"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VF995_08260"
     CDS             <1..736
                     /locus_tag="VF995_08260"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007469784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX9343589.1"
                     /translation="SRPGMALIAEVKRRSPSKGDIRADLDPAGLARAYQRGGAAAISV
                     LTERRHFSGSPDDLRATRTATDLPVLRKDFITTPYQLCEARAWGADAVLLIVAALDPE
                     QLASLLAEAALAGLDALVEVHTRAEAETAAAAGATLVGINARDLTTLTVDPRRFAAVR
                     GALPADGVTVVAESGIHDRNGVLAAAAAGADAVLVGESLVRADDPAAAAAALLGRIPP
                     TATPTPSHQVAPQGDGAAAPIGGHDG"
     gene            729..2036
                     /locus_tag="VF995_08265"
     CDS             729..2036
                     /locus_tag="VF995_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX9343590.1"
                     /translation="MAEPRKVLLSEDELPTAWYNIVPDLPVKPDPYLHPGTREPVTPD
                     LLTPLFPMALIGQEVSTDRWIDIPGAVLDTYRIWRPTPLIRALDLERALGTPARIYYK
                     YEGGSPAGSHKPNTAVAQAWYNKAEGTTSLSTETGAGQWGSALAFACARFGLDCKVYM
                     VRASYDSKPYRRSLMETWGASVTPSPSPETAAGRAVLDADPDSPGSLGIAISEAVEVA
                     AQDPGIHYALGSVLNHVLLHQTIIGLELKAQLGKLGVTPDVLIGCVGGGSNWGGFSFP
                     LLADKLAGRIATRFVAVEPTAAPTLTKGRFDYDLGDQAGMTPLIPMFTLGSDFIPAPI
                     HAGGLRYHGDSPLISLLVRDGHMEAVAYPQTKVFEAAVQFARTEGILPAPEPAHCIRA
                     AIDEALRCKEQDSAETIVFNLCGHGHFDLKAYEDYLSGDMLDV"
     gene            2029..3375
                     /gene="trpB"
                     /locus_tag="VF995_08270"
     CDS             2029..3375
                     /gene="trpB"
                     /locus_tag="VF995_08270"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX9343591.1"
                     /translation="MSDPSDPNQNTPAGAGHPRGHGQGSGQGVLLDLPALRRAIPHPP
                     DPFGRFGPYGGRYVPEVLMAALDELTAAFEEAMADPSFNAELDELRRAYAGRPTPLTP
                     ARRLSKELGLTVWLKREDLLHTGAHKLNNSLGQHLLTKRMGKPRVIAETGAGQHGVAT
                     ATAAALLGQDCVVYMGEEDTRRQHLNVVRMRLLGTEVRPVATGTATLKDAINEALRDW
                     VESVGHTHYAIGSVVGPHPFPLMVREFQRVIGEEARAQLLDAAGRLPDLAVACVGGGS
                     NAAGLFAPFVDDATVRLVGVEAGGRGPGPGDNGAPLNFGQAGVLHGARSYLLQDPDGQ
                     VLPTHSVSAGLDYPGVGPEHALWKDDGRVTYTEAGDRAALDGFQLLARTEGILPALEP
                     AHAIGWLAAAARAGTVTPGSLVVLNLSGRGDKDVETVASILGMDAEENAEGGRHDG"
     gene            3368..>3907
                     /gene="trpA"
                     /locus_tag="VF995_08275"
     CDS             3368..>3907
                     /gene="trpA"
                     /locus_tag="VF995_08275"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX9343592.1"
                     /translation="MAEGRTPTGAFTSTAAPKAGIPGGAGAGDTGEGLPPADRLEGRL
                     ATLRAAGRAALVAYGVAGYPDRAASLAAFQAMAAAGADVLEVGPPYSDPLIDGPVIQR
                     AVQAALDAGTRLDDVFALVGELTATVDTPVVLLVYYNLIAHRGPARFAADTAAAGACG
                     AVVPDLPPEEAGEWLAAAEA"
BASE COUNT          551 a         1581 c         1274 g          501 t
ORIGIN      
        1 gtcgaggccg ggcatggcgc tgatcgccga agtcaagcgg cgctcgccct ccaaaggaga
       61 catccgcgcc gacctggacc ctgccggact cgcccgcgcc taccagcgcg gcggcgccgc
      121 cgccatcagc gtcctcaccg aacggcgcca cttctccggc agccccgacg acctgcgcgc
      181 cacccgcacc gccaccgacc tgccggtcct gcgcaaggac ttcatcacca ccccctacca
      241 gctctgtgag gcacgggcct ggggtgcgga cgcggtcctg ctgatcgtcg ccgccctcga
      301 ccccgaacag ctcgcctccc tgctcgccga ggccgccctg gccgggctcg acgcgttggt
      361 cgaggtccac acccgcgccg aggccgaaac cgccgccgcc gcaggcgcga ccctggtcgg
      421 catcaacgcc cgcgacctca ccaccctcac cgtcgacccg cgccggttcg ctgccgttcg
      481 cggcgccctg ccggctgacg gggtcaccgt ggtcgccgag agcggcatcc atgaccgcaa
      541 cggcgtgctt gccgcagccg ccgccggtgc cgatgctgtg ctggtcggcg agtccctggt
      601 ccgcgccgac gacccggcag cggccgccgc cgcgctgctc ggccggatcc cacccacagc
      661 cacacccaca ccatcacatc aggtagcgcc acagggagat ggggccgcag ccccgatcgg
      721 aggacatgat ggctgagccg cgcaaggtcc tgctgagcga ggacgagctg cccacggcct
      781 ggtacaacat cgtcccggat ctgcccgtca agcccgaccc ctacctgcac ccggggaccc
      841 gggagccggt cacccccgac ctgctcacgc cgctgttccc gatggcgctg atcggccagg
      901 aggtctccac cgaccgctgg atcgacatcc ctggcgcggt gctcgacacc taccggatct
      961 ggcggccgac gccgctgatc cgcgccctcg acctggaacg tgctctcggg acacccgcgc
     1021 ggatctacta caagtacgaa ggcggctccc cagcgggctc ccacaagccc aacaccgccg
     1081 tcgcccaggc ctggtacaac aaggccgaag gcacgacgag cctgtccacc gagaccggcg
     1141 ccggccaatg gggcagcgcg ctggccttcg cctgcgcccg cttcggcctg gactgcaagg
     1201 tctacatggt gcgggcctcc tacgacagca agccgtaccg gcgctcgctg atggagacct
     1261 ggggcgcctc ggtgaccccg tcgccgtcgc cggagaccgc cgccggccgc gccgtgctcg
     1321 acgccgaccc cgactcgcca gggtcactcg gcatcgccat ctccgaagcc gtcgaggtcg
     1381 ccgcccagga cccgggcatc cactatgcgc tcggcagcgt cctcaaccac gtgctgctcc
     1441 atcagacgat catcggcctg gaactgaagg cccagctcgg caagctcggc gtcacccccg
     1501 acgtgctgat cggctgtgtc ggaggcgggt ccaactgggg tgggttctcg ttcccgctgc
     1561 tggccgacaa gctcgccggg cggatcgcca cccgcttcgt cgccgtcgag ccgaccgcag
     1621 cccccaccct caccaagggg cgcttcgact acgacctcgg cgaccaggcc gggatgacgc
     1681 cgctgatccc gatgttcacc ctcgggtcgg acttcatccc cgcgccgatc cacgccggcg
     1741 ggctgcgcta ccacggcgac tcgccgctga tctccctgct ggtcagggac ggccatatgg
     1801 aggcggtcgc ctacccgcag accaaggtct tcgaggccgc tgtccagttc gcccgcaccg
     1861 agggcatcct gcccgcgccg gagccagccc actgcatccg cgccgcgatc gacgaggcgc
     1921 tgcgctgcaa ggagcaggac tcggccgaga ccatcgtgtt caacctctgc ggccacggcc
     1981 acttcgacct caaggcctac gaggactacc tgtcagggga catgctcgat gtctgacccg
     2041 tccgacccga accagaacac gcccgccgga gccgggcacc cgcggggaca cgggcaggga
     2101 tctgggcagg gggtgctgct cgacctgccc gccctgcgcc gcgccatccc gcacccaccc
     2161 gacccgttcg ggcgcttcgg cccctatggc ggccgttacg tgcccgaggt gctgatggcc
     2221 gccctcgacg agctgaccgc cgcgttcgag gaggccatgg ccgatccgag cttcaacgcc
     2281 gagctggacg agctgcgccg cgcctacgcc gggcggccaa ccccgctcac cccggcccgg
     2341 cggctctcga aggagctggg gctgaccgtc tggctcaaac gcgaggacct gctgcacacc
     2401 ggcgcccaca agctcaacaa ctccctcggc cagcacctgc tcaccaagcg catgggcaaa
     2461 ccccgggtga tcgctgagac cggcgctggc cagcacggcg tcgccaccgc caccgccgcg
     2521 gccctcctcg gccaggactg cgtcgtctac atgggcgagg aggacacccg ccgccagcac
     2581 ctcaacgtcg tccgcatgcg cctgctcggc accgaggtcc gcccggtcgc caccggcacc
     2641 gccaccctca aggacgccat caacgaggcg ctgcgcgact gggtcgagtc ggtcggccac
     2701 acccactacg ccatcggctc ggtggtcgga ccgcacccgt tcccgctgat ggtccgcgag
     2761 ttccagcgag tcatcggcga agaagctcgc gcccaactgc tcgacgccgc cggccgcctg
     2821 cccgacctcg ccgtcgcctg cgtcggcggc gggtccaacg ccgccgggct gttcgccccg
     2881 ttcgtcgacg acgccactgt ccggctggtc ggcgtcgagg ccggtggccg cgggcccggg
     2941 cccggcgaca acggcgcccc gctcaacttc ggccaggccg gcgtcctcca cggggcccgc
     3001 tcctacctgc tccaggaccc cgacgggcag gtcctgccta cccactcggt gtcggccggg
     3061 ctcgactacc ccggcgtcgg ccccgagcat gccctctgga aggacgacgg ccgcgtcacc
     3121 tacaccgagg ccggcgaccg agccgccctc gacggcttcc agctccttgc ccgcaccgag
     3181 gggatcctgc ccgcgctgga acccgcccac gcgatcggtt ggctcgccgc cgccgcccgc
     3241 gcagggaccg tcacacccgg gtctttggtg gtgctgaacc tctccggccg gggcgacaag
     3301 gacgtcgaga cagtcgccag catcctcggc atggacgccg aggagaacgc ggaagggggg
     3361 cggcacgatg gctgagggtc ggacaccgac cggggcgttc acctccacgg ccgctcccaa
     3421 ggcggggatt ccgggtggag cgggcgcggg ggacacgggt gagggcctgc cgcccgccga
     3481 ccggctggag ggccggctcg ccaccctgcg ggcggcggga cgggctgcgc tggtcgccta
     3541 cggggtcgcc gggtatcccg accgggccgc ctcgctggcc gccttccagg ccatggccgc
     3601 cgcgggcgcc gacgtccttg aggtgggccc gccctactcc gacccgctga tcgacgggcc
     3661 ggtcatccag cgcgccgtcc aggcggcgct cgacgccggc acccgcctgg acgacgtgtt
     3721 cgccctggtc ggcgagctga ccgccaccgt cgacaccccg gtcgtcctgc tcgtctacta
     3781 caacctcatc gcccaccgcg gcccggcccg cttcgccgcc gacaccgccg ccgctggggc
     3841 ctgcggcgcg gtcgtccccg acctgccgcc ggaggaggcg ggggagtggc tcgccgccgc
     3901 cgaggcc
//
LOCUS       DASWDC010000254         4365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1400678, whole genome shotgun sequence.
ACCESSION   DASWDC010000254 DASWDC010000000
VERSION     DASWDC010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400678"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..543)
                     /locus_tag="VF995_08280"
     CDS             complement(58..543)
                     /locus_tag="VF995_08280"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF000832.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX9343593.1"
                     /translation="MIVGVGVDAVEIDRVARALQRHPGFGARVFTPAELQSAGRRGAG
                     RVAYLAKRWAAKEAVSKALGVGFSGLSYTDIEVTNLRSGAPTVTVHGEVAEWSHQLGV
                     LHWHLSLSDTRDTAVAMAVAEGPDDYPRHPTTPPPWVARRLERQQQRQRQRGRERERR
                     P"
     gene            complement(540..872)
                     /locus_tag="VF995_08285"
     CDS             complement(540..872)
                     /locus_tag="VF995_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343594.1"
                     /translation="MPFNIDPEQSVSIVAVFLFIDAALIGFPLLSALIGGGGAAPLGL
                     VLLAGLIAAGVGILKGRQWAWYLAVGVVGLKLLFDLAGIVLLALIFDGLAMFLLVQPG
                     LRSRFGVR"
     gene            complement(1025..2347)
                     /locus_tag="VF995_08290"
     CDS             complement(1025..2347)
                     /locus_tag="VF995_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEX9343595.1"
                     /translation="MSTAATEGLPAAPTPQSSVLAVLQRIGRSLMLPIAVLPAAALLL
                     RFGQPDLLVKVWSHFSILATAGNALFSNLPILFAVGVAVGFARRSDGSTALAALVGYL
                     IFDRVTMAIFATTSLSTQVLSTSADFKTPALAIEKAPANPTGVIGGIIIGVTAALLYN
                     RFYRIKLPNWLAFFGGRRFVPIVTAVAALILGVIFGYIWTPIGHGINNLGDLLTTHGA
                     VGAGIYGMLNRLLLPFGLHHIVNSLVWFTFGTYNGVHGDLNRYFAGDPTAGTFMAGFF
                     PVMMFGLPAACLAIWRNAHPSQRRVIGGIMLSAALTSLVTGVTEPIEFAFLFVAPVLY
                     GVHVVLTGVSMWLAAALGVHDGFGFSAGLTDYVLNFTKSNTAKPLLLLLIGAVYAVVY
                     YFLFDFLIKRFNLQTPGREPDTAAVETPEQPAPAPAPGSGSRQEEAPA"
     gene            2494..3714
                     /gene="nagA"
                     /locus_tag="VF995_08295"
     CDS             2494..3714
                     /gene="nagA"
                     /locus_tag="VF995_08295"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX9343596.1"
                     /translation="MPVISAPRVVAQGRVLAPGAVVIEDGHVAAVLGTRPRPAPDHLA
                     LDRGILCPGLVDLQVNGAFGVDLIDADRDGWSRVSRGLLGTGVTSFLPTFISAPIETL
                     AAGLRRAGAARDQRDQMEAAGRAGSPGARILGVHLEGPFLSERRRGAHDPAALVDPTT
                     ERVEALLEAGAGILRLVTLAPEREGALEAVKRLTAGGVAVSVGHSDALAEPVAAAADA
                     GARMITHVFNAQRGLHHREPGVAGQALADEQLTVGLIADLHHVHPAVCRIVLAAAAGR
                     VALVTDAVAATGMPPGRYQLGGELVELGPDGPPRRPDGTIAGSNLRLDQAVGNVAGLL
                     SGTGTDADAGTDADAEAGLAAAVEAASRVPADLLGRTDLGRIEVGALADLAWLSDTYH
                     AVGTWIEGVQAWRA"
     gene            3711..>4365
                     /locus_tag="VF995_08300"
     CDS             3711..>4365
                     /locus_tag="VF995_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX9343597.1"
                     /translation="MNAGTPTRMAAEIAEQPEAVARTLAALRPQRAELRRLAEGVRLV
                     SFIARGSSDNAATYGRYLCEVHAGRAASLAAPSVATHYHADVDLRGVLAVALSQSGET
                     AEIVETLQWAAKHGARTVAVTNDQASTLAAAADLALVTRAGPELAVPATKTYSCQLAA
                     LSELAAALGRDDPAFDAASERVPAAIEALLACAPAAAALATEIAGAASLVVSGRGFAY
                     "
BASE COUNT          649 a         1575 c         1570 g          571 t
ORIGIN      
        1 cggcggcctt ggcggcggcg tcggcggcct ggatcgtggg cacctcgacc aggggcttca
       61 tggccgccgc tcccgctccc gcccccgctg gcgctggcgc tgctgctggc gctccagccg
      121 ccgggccacc cagggcggcg gggtggtcgg atggcggggg tagtcgtccg gcccctcggc
      181 gacggccatg gcgacggcgg tgtcgcgggt gtcggacagc gacagatgcc agtgcagcac
      241 cccgagctgg tgtgaccatt cggccacctc gccgtggacg gtgacggtcg gggcgccgga
      301 acgcaggttg gtgacctcga tgtcggtgta ggagaggccg gagaagccca ccccgagggc
      361 tttggagacg gcctccttgg cggcccagcg tttggcgagg taggcgaccc ggccggcgcc
      421 gcggcgcccg gcgctctgaa gttccgccgg tgtgaagacc cgcgcgccga agccagggtg
      481 gcgttggagc gcgcgggcca cccggtcgat ctcgaccgcg tccaccccca cccccacgat
      541 catcggaccc cgaaccggga tcgcaaccct ggctgcacca gcaggaacat cgccagccca
      601 tcgaagatca gcgccagcag gacgatgccg gccaggtcga acaggagctt gagcccgacc
      661 acgccgactg ccaggtacca ggcccactgg cgtcctttga ggatgccgac tccggcggcg
      721 atcagcccgg cgagcagcac caaccccagc ggcgccgcgc cgccaccccc gatcagcgcg
      781 gacaacaggg gaaagccgat cagcgcggcg tcgatgaaca gaaagaccgc gacgatggag
      841 accgactgct cgggatcgat gttgaacggc atggcactct cccggccggg accgctgtcg
      901 gactggcagc ttgcttgttt ccagtgtcct acgtgccgaa tcgtagcagc ggtcccggac
      961 cggattggcg gttatccaca acccctgttg atccgatttt ccggatcggg gcgtggtcga
     1021 tggctcaggc gggcgcctcc tcctgccgcg acccggaccc cggcgcgggt gccggcgcag
     1081 gctgctctgg cgtctcgacc gccgccgtgt ccggttcgcg accgggggtc tgcaggttga
     1141 accgcttgat caggaagtcg aacaggaagt agtagacaac cgcgtagacc gcgccgatca
     1201 gcagcagcaa cagcggcttg gccgtgttcg acttggtgaa gttgagcacg tagtcggtca
     1261 ggccggcgga gaacccgaag ccgtcgtgga cgccgagcgc ggccgccagc cacatcgaca
     1321 cccctgtcag caccacgtgg accccataca gcaccggggc gacgaacagg aaggcgaact
     1381 cgatcggctc ggtgacgccg gtgaccagcg aggtgagcgc ggccgacagc atgatcccgc
     1441 cgatcaccct gcgctgcgac gggtgggcgt tgcgccagat cgccaggcag gcggcgggca
     1501 ggccgaacat catcaccggg aagaacccgg ccatgaaggt ccccgcggtc gggtcgccgg
     1561 cgaagtagcg gttcaggtcg ccgtggacgc cgttgtaggt gccgaaggtg aaccacacca
     1621 gcgagttgac gatgtggtgc agcccgaacg gcagcagcag ccggttgagc atcccgtaga
     1681 tgcccgcgcc gacggcgccg tgggtggtga gcaggtcgcc gaggttgttg atgccgtggc
     1741 cgatcggcgt ccagatatac ccgaagatca cccccaggat cagcgcggcg acggcggtga
     1801 cgatcgggac gaagcggcgc ccgccgaaga acgccagcca gttgggcagc ttgatgcggt
     1861 agaagcggtt gtagagcagc gcggcggtga cgccgatgat gatcccgccg atcaccccgg
     1921 tcgggttggc gggggccttc tcgatcgcca gcgcgggtgt cttgaagtcc gcggaggtcg
     1981 acagcacctg ggtgctgagc gaggtggtgg cgaagatcgc catcgtcacc cggtcgaaga
     2041 tcaggtagcc gaccagcgcg gccagcgcgg tcgagccgtc ggagcggcgc gcgaacccga
     2101 ccgcgacgcc caccgcgaac aggatcggca ggttcgagaa cagcgcgttg ccggcggtcg
     2161 cgaggatgct gaagtggctc cagaccttga ccagcaggtc aggctggcca aagcgcagca
     2221 gcagcgcggc ggccggcagc accgcgatcg gcagcatcag gctgcggccg atgcgctgca
     2281 gcacggcaag taccgaggat tggggggttg gggcggccgg gaggccttcg gtggcggctg
     2341 tggacatgcg gtctcccctc catctggatg agtggcatcc acagagcaat gccgggactc
     2401 tgcatctgga ctagaccaca ttaaggtctc tcaagtaaca cccgctgcgc aaggtcattg
     2461 ccttactgct caagccgact aagctggagg cccatgccag tcatctccgc tccccgcgtg
     2521 gtcgcccaag gccgggtgct cgcccccggc gcggtcgtga tcgaggacgg ccacgtcgcg
     2581 gcggtgctcg gcacccggcc gcgcccggca cccgaccacc tcgccctcga tcggggcatc
     2641 ctctgccccg gcctggtgga cctgcaggtc aacggcgcct tcggggtgga cctgatcgac
     2701 gctgaccggg acggctggtc ccgggtcagc cgggggctgc tcgggaccgg ggtgaccagc
     2761 ttcctgccga ccttcatcag cgccccgatc gagaccctgg cggctgggct ccgccgcgcc
     2821 ggcgccgcgc gggaccagcg ggaccagatg gaggccgccg ggcgagccgg atcacctggc
     2881 gcgcggatcc tcggggtgca tctggagggg ccgttcctgt cggagcgccg acgcggcgcc
     2941 cacgacccgg ccgcgctggt cgacccgacc accgagcgtg tcgaggcgct gctggaggcg
     3001 ggcgcgggca tcctgcgcct ggtgacgctg gcgcccgaac gggagggggc cctggaggcg
     3061 gtcaagcgcc tcaccgccgg cggcgtggcc gtctccgtcg gccactcgga cgcgctggcg
     3121 gaaccggtcg ccgccgccgc tgacgcgggc gcccgcatga tcacccacgt cttcaacgcg
     3181 cagcggggcc tgcaccaccg cgaaccgggc gtcgccggcc aggcgctcgc cgacgagcag
     3241 ctcaccgtcg gactgatcgc cgacctgcac cacgtccacc cggcggtctg ccggatcgtc
     3301 ctggcagccg ccgccggacg ggtggccctg gtgaccgacg cggtcgcggc caccggcatg
     3361 ccgcccggcc gctaccagct cggcggcgag ctggtcgaac tcgggccaga cggtccgccc
     3421 cggcggcccg acggcaccat cgccggctcg aacctccgcc tcgaccaggc ggtcggcaac
     3481 gtcgccggtc tcctctccgg caccggcact gacgctgacg ccggcactga cgctgacgcc
     3541 gaagccgggc tggccgccgc ggtcgaggcg gccagccgcg tcccggccga cctgctgggg
     3601 cgcaccgacc tcggccgcat cgaggtgggc gcgcttgctg acctcgcctg gctgtcggac
     3661 acctaccatg ccgttggcac ctggatcgag ggagtgcagg catggcgggc atgaacgcgg
     3721 ggacgcccac ccggatggcc gctgagatcg ccgagcagcc cgaggcggtc gcccgcaccc
     3781 tggcggcgct gcggccgcag cgggcggagc tgcgccgcct cgctgagggc gtgcggctgg
     3841 tgagcttcat cgcccggggc tcctccgaca acgccgccac ctacgggcgc tacctctgcg
     3901 aggtccatgc cggccgtgcg gcgagcctgg ccgcgccctc ggtcgccacc cactaccacg
     3961 ctgacgtgga cctgcgcggc gtgctcgcgg tggcattgtc gcagtccggc gagaccgccg
     4021 agatcgtgga gaccctccag tgggcggcca agcacggcgc ccgcaccgtg gcggtcacca
     4081 acgaccaggc cagcaccctt gccgccgccg ccgacctcgc cctggtcacc cgcgccggcc
     4141 cggagcttgc cgtgcccgcc accaagacct acagctgcca gctcgcggcg ctcagcgagc
     4201 tggccgccgc cctcggccgc gacgaccccg ccttcgacgc cgccagcgaa cgcgtccccg
     4261 ccgcgatcga ggcgctgctc gcctgcgccc cggccgccgc cgcgctcgcc accgagatcg
     4321 ccggggcggc cagcctggtc gtctccggcc gcggcttcgc ctact
//
LOCUS       DASWDC010000255         8832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_568692, whole genome shotgun sequence.
ACCESSION   DASWDC010000255 DASWDC010000000
VERSION     DASWDC010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568692"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VF995_08305"
     CDS             <1..1182
                     /locus_tag="VF995_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX9343598.1"
                     /translation="VLLVDDYQEARDNLGRVLDRLGGIKVVGLAGDGAEALDLAEKVA
                     PQVVLMDLRMPRMDGVEATRLLAERHPAIAVLILSAYGDEGLVIDALTAGARGYLLKG
                     TQAPQLAEAIVGAANGQARIAGQVTRPILERLVEALGQERETARLAQEARQASERISR
                     RQQQFATVAAHELRTPVTAVLGSLTTLERLIPPEELPPVAGDMLSACTRQARRLARLV
                     EDLTVVAKGASGGITVKPAMVDVGETIASVVADLDHLGAERVRVNAAPGLVAWADPDR
                     YTQVLTNLVRNALQYSPPGEPVEVSASAAPGVVRVAVADRGPGIPAERLPELFDRFGD
                     RGKTHATGLGVGLWIVRELLNAMHGNVWVEANPEGGSIFYSAIPAHNRSADWDRDPDL
                     S"
     gene            1366..2142
                     /locus_tag="VF995_08310"
     CDS             1366..2142
                     /locus_tag="VF995_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005312455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343599.1"
                     /translation="MAVRSVAERTPGARFATLRKDRWWLQPLVTVVVLAGFVVYGIYV
                     AFVNKDYYHDPYISPFYSPCLASSCAHATAKIFGDWWKLSPALLVLPFPLGFRLTCYY
                     YRKAYYRSFWWAPPACAVPDARPRYSGETRFPLILQNIHRYFFYLALPFPILLTWDAL
                     KAFKFPDGFGLGVGTLVLLLNAGMLWLYTLSCHSCRHLCGGHLNTFSAHPLRYRLWRW
                     STVLNKRHMLYAWVSLFVVALADLYIRLVANGTIHDPRLF"
     gene            2145..3962
                     /locus_tag="VF995_08315"
     CDS             2145..3962
                     /locus_tag="VF995_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX9343600.1"
                     /translation="MPEYETHEFDVVVVGAGGAGLRAAIEAAAQGCQVALVCKSLLGK
                     AHTVMAEGGIAAAMGNVYPDDNWRVHFRDTMRGGKMLNNWRMAQLHAQEAPDRVYELE
                     EWGALFDRTKDGRILQRDFGGHRYARLAHVGDRTGLEMIRTLQHRVVGNSGGAVQVFM
                     ECTVTRLLTDPDGRIAGAFGYWRESGRFLVFNAKAVVVATGGIGKAWKVTSNSWEYTG
                     DGHSLALWAGADLIDMEFVQFHPTGMVWPPSVRGILVTEGVRGDGGVLRNSEGRRFMF
                     DYIPEMFRAETADTEEEADRWYKDRKSARRTPDLLPRDEVARAINNEVKEGRGSPHGG
                     VFLDIASRLDAESIRRRLPSMYHQFKQLADVDITKEPMEVGPTCHYVMGGVRVDADTE
                     AATVPGLFAAGEAAGGMHGANRLGGNSLSDLLVFGRRAGLGAAEYAKGLAAAPRADPL
                     QAEAMAAEALALFEREAGENPYTIQHDLQDTMQELVGIIRTEGELKAALEKLQVLKER
                     AGQVRVEGNRQYNPGWHLALDLRSLLTVSECVALAALERKESRGGHTRDDHPKPDPEF
                     GRLNMALRQKEPGGEIALAREPLPEMPDDLKQLFDEGKG"
     gene            3969..4775
                     /locus_tag="VF995_08320"
     CDS             3969..4775
                     /locus_tag="VF995_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX9343601.1"
                     /translation="MPGDNGNGTASANGNGSGGYDVTLRLWRGDESGGELRDYTVPVE
                     EGEVVLDAVHRVQATQAGDLAVRWNCKAGKCGSCSAEVNGKPRLLCMTRLSVFERGEP
                     ISITPLRTFPIIRDLVTDVSFNYEMAKKVPPFQPRPREADGTYRMMQEDIDRAQEFHK
                     CIECFLCQNVCHVIRDHEDNKPAFAGPRMFIRYAELEMHPLDTNDRRALLREQGGLGY
                     CNITKCCTEVCPEEIKITDNAIIPLKERVVDRSYDPLARLLKRLKGDKAD"
     gene            4900..5514
                     /locus_tag="VF995_08325"
     CDS             4900..5514
                     /locus_tag="VF995_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343602.1"
                     /translation="MPEPVLRREHFRDAEVRQFRPRADPAPAPGPDPGANPKPRHVRD
                     LSPVHEAVTTQLLVVSVAGLSAGAAAIHFAIVQSHLKELPAAGVFFLALAVAQALWAI
                     AIPRYHSRQMLAWGAVANLAIVALWLWVRVYGLPVGPEPWVPEAFGITDTISATFELT
                     LALAATALLTNLRLPLRRLTVPASLLALTVALGTWIALLHASMT"
     gene            5559..5807
                     /locus_tag="VF995_08330"
     CDS             5559..5807
                     /locus_tag="VF995_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343603.1"
                     /translation="MVKILVSIDDQLLQRIDRAAQRLGLTRSAWLSRVAARDVGDEHG
                     PGTDQRARRAMAALDQLFQRQSVPEDATAAIRADRDTR"
     gene            5804..6208
                     /locus_tag="VF995_08335"
     CDS             5804..6208
                     /locus_tag="VF995_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9343604.1"
                     /translation="MTEVVLDASVVLKWFTPGEEPGRSEARALRRDYDAGHLTVVVPG
                     LFFLELLNVAGRRWGWDGPALRELADDLDDLLFDTAEPELPSVATWIARGLTAYDAAY
                     VALAEERSISLVTADDGILKLAGTIARPLVPH"
     gene            complement(6281..6649)
                     /locus_tag="VF995_08340"
     CDS             complement(6281..6649)
                     /locus_tag="VF995_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343605.1"
                     /translation="MEAMMWWRVICLTLLGLFGLTACGTSPLRGAAPGVLTGGIVYHG
                     GPSSAAPSNTHYEPGTVEVRHQGRLVTTQQVSEGNLYHLSLAPGDYDLYVHLGDLTCH
                     RLVRVQSASTTTADLSCSIK"
     gene            complement(6717..8369)
                     /locus_tag="VF995_08345"
     CDS             complement(6717..8369)
                     /locus_tag="VF995_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343606.1"
                     /translation="MGVETAGPDAAIRAGPPGAGVPARVPADRCADPHRDPDQPPGRR
                     GRGRRWWDRWWLDALLLAPILLVAWFAELPRDMLSRSFWLDEGWVADSLRAPIGQLKL
                     LASSTPVGWELLLRLVPHVGLPERLRLVPMLFAVAAAAAGWLFGRQFGRIQGVAVGLA
                     VAVAPGMLMMRSLKQYTAEAFVALLLVWLASRVEERWSPRRVVVLGVACLPAIFLSNT
                     TVFVSAALFGALGLLALPRRDWRKLGWLAAVGAGALAVEGTVYLTFVGTGNNAGMQRY
                     WGRFFVPLTSGRGVAVHVIASQGHLLLGRIGFGPALLSAALVVAGLVVLWRAGRPAVA
                     TSVVLLAFAQLLAGIAHRYPLFDVRTSMFYAVLLTACAALAVGSFAAWAWRRLLTAPL
                     GVVAVGLAAVLLVPAAHTAQHSRLPPSRMRQQVDDVLAHRRPGDVVVVGWVATFPFAY
                     YWPEQPSFPPTDLNTAVLFKVAYPNTPDVVVAHRNNSEADDEAALRTALARIQASGGT
                     GRIWLVLADSGDGTTQWPAAIKATGIHLVSHARPILLTTGTS"
     gene            8605..>8832
                     /locus_tag="VF995_08350"
     CDS             8605..>8832
                     /locus_tag="VF995_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343607.1"
                     /translation="MNTQIRGAVRRTEDQRLVTGSARYTEDLEIPGALHAAFVRSAVA
                     HGRIVAIDTEEAKAAPGVAGVYTAADLELGTL"
BASE COUNT         1388 a         3283 c         2926 g         1235 t
ORIGIN      
        1 gtgctgctgg tcgacgacta ccaggaggcc agggacaacc tcgggcgggt gctcgaccgg
       61 ctcggcggca tcaaggtcgt cggcctggcc ggcgacggcg ccgaggcgct cgacctggcg
      121 gagaaggtgg cgccgcaggt cgtgctgatg gacctgcgca tgccccggat ggacggggtg
      181 gaggcgaccc ggctgctggc ggagcgccac ccggcgatcg ccgtgctgat cctgtcggcc
      241 tatggcgacg aggggctggt gatcgacgcg ctcaccgccg gcgcccgcgg ctacctgctg
      301 aagggcacgc aggcgccgca gcttgccgag gcgatcgtcg gcgccgccaa cggccaggcg
      361 cggatcgccg gccaggtgac ccgcccgatc ctggagcggc tggtcgaggc gctcggccaa
      421 gagcgcgaga ccgcccgcct cgcccaggag gcacgccagg ccagcgagcg gatctcgcgc
      481 cgccagcagc agttcgccac cgtggcagcc cacgagctgc gcaccccggt gaccgccgtg
      541 ctcggctcgc tgacaacgct ggagcgcctc atcccaccgg aggagctccc gccggtggcc
      601 ggcgacatgc tgtcggcctg cacccgccag gcacgccgcc tcgcccgcct ggtcgaggac
      661 ctgaccgtgg tcgccaaggg ggccagcggc ggcatcaccg tgaagcccgc catggtcgac
      721 gtgggcgaga cgatcgcgag cgtggtcgcc gacctcgacc acctcggcgc cgagcgggtc
      781 cgcgtcaacg ccgcccccgg cctggtcgcc tgggcggacc cggaccgcta cacgcaggtg
      841 ctcaccaacc tggtccgcaa cgccctgcag tactccccgc ctggcgagcc ggtggaggtg
      901 agcgccagcg ccgcgcccgg cgtggtccgg gtcgccgtgg ccgaccgagg tcccggcatc
      961 cccgccgaac gcctcccgga gctgttcgac cgcttcggcg accgcggcaa gacccacgcc
     1021 accggcctcg gcgtcggcct gtggatcgtc cgcgagctgc tcaacgccat gcacggcaac
     1081 gtctgggtcg aagccaaccc cgagggcggc tcgatcttct acagcgccat ccccgctcac
     1141 aaccgctccg ccgactggga ccgcgacccg gatctctcct gatccccgtg atcccggccc
     1201 ccatccccat cctctgattc cggccgagcc tgtggatacg gcgatgctga ccttggtcgg
     1261 gcggactatg ctgcccgatg gaaaatccga catggagaga agctgccggg tgaccccagg
     1321 tcaccggcct gtcaagccaa tacggctgaa aggtcagagg agacgatggc tgttcgcagc
     1381 gtcgcggaac gaaccccggg ggcccgcttc gccaccttgc ggaaagaccg ctggtggctc
     1441 caaccgctgg tcacggtcgt ggtgctggcc ggcttcgtgg tctacggcat ctacgtggcg
     1501 ttcgtgaaca aggactacta ccacgacccc tacatctcgc cgttctattc tccctgcctg
     1561 gcctccagct gcgcccacgc caccgccaag atcttcggcg actggtggaa gctgtccccg
     1621 gcgctgctgg tgctgccgtt cccgctcggc ttccggctca cctgctacta ctaccgcaag
     1681 gcgtactacc gctcgttctg gtgggcgccg cccgcctgcg ccgtcccgga cgcgcggccg
     1741 cgctacagcg gcgagaccag gtttcccctg atcctgcaga acatccaccg ctacttcttc
     1801 tacctggcgc tgccgttccc gatccttttg acctgggacg cgctgaaggc gttcaagttc
     1861 cccgacggct tcggcctcgg tgtcgggacc ctggtgctgc tgctcaacgc gggcatgctg
     1921 tggctctaca cgctgtcgtg ccactcctgc cgccacctct gcggcggcca cctcaacacg
     1981 ttctccgcgc acccgctccg ctaccggctg tggcgctgga gcaccgtgct caacaagcgc
     2041 cacatgctgt acgcgtgggt gagcctgttc gtggtcgccc tcgccgatct gtacatccgc
     2101 ctggtcgcca acgggaccat ccacgacccg aggttgttct agccatgccc gagtacgaga
     2161 cccacgaatt cgacgtcgtc gtcgtcggcg ccggcggcgc cgggctgcgc gcggcgatcg
     2221 aggcggccgc gcagggctgc caggtcgccc tggtctgcaa gtcgctgctc ggcaaggccc
     2281 acaccgtcat ggccgagggt ggcatcgccg ccgccatggg caacgtctac cccgacgaca
     2341 actggcgcgt ccacttccgc gacaccatgc gcggcggcaa gatgctcaac aactggcgga
     2401 tggcccaact ccacgcccag gaggcccccg accgggtcta cgagctggag gaatgggggg
     2461 cgctgttcga ccgcaccaag gacggccgca tcctgcagcg cgacttcggc ggccaccgct
     2521 acgcccgcct cgcccacgtt ggcgaccgca ccggcctgga gatgatccgc accctgcagc
     2581 accgcgtggt cggcaacagc ggcggagcgg tccaggtgtt catggagtgc accgtcaccc
     2641 gcctgctcac cgaccccgac ggccgcatcg cgggcgcctt cggctactgg cgcgaatccg
     2701 gccgcttcct cgtcttcaac gccaaggcgg tggtggtcgc cactggcggg atcggcaagg
     2761 cctggaaggt cacctccaac tcctgggagt acaccggcga cggccactcg ctggcgctgt
     2821 gggccggggc ggacctgatc gacatggagt tcgtccagtt ccaccccacc ggcatggtct
     2881 ggcccccctc ggtgcgcggc atcctggtca ccgagggtgt gcgcggcgac ggcggcgtgc
     2941 tgcgcaactc cgagggccgg cgcttcatgt tcgactacat ccccgagatg ttccgcgccg
     3001 agaccgccga caccgaggag gaggcggacc gctggtacaa ggaccgcaag tccgcccgcc
     3061 gcacccccga cctgctcccc cgtgacgagg tcgcccgcgc gatcaacaac gaggtcaagg
     3121 agggccgcgg cagcccccac ggcggggtct tcctggacat cgcctcgcgg ctggacgccg
     3181 agtcgatcag gcggcgcctg ccgagcatgt accaccagtt caagcagctc gccgacgtcg
     3241 acatcaccaa ggagccgatg gaggtcggcc ccacctgcca ctacgtgatg ggcggtgtcc
     3301 gcgtcgacgc cgacaccgag gccgccaccg tccccggcct gttcgccgcg ggcgaggcgg
     3361 ccggcgggat gcacggcgcc aaccggctcg gcggcaactc cctctcggac ctgctggtgt
     3421 tcggccgccg cgccggcctc ggcgccgccg agtacgccaa gggcctcgct gccgcgccgc
     3481 gggccgaccc gctccaggcc gaagcgatgg ccgccgaggc gctggccctg ttcgagcggg
     3541 aggcgggcga gaacccctac accatccagc acgacctcca ggacaccatg caggagctgg
     3601 tcggcatcat ccgcaccgag ggcgagctca aggccgccct cgagaagctc caggtcctca
     3661 aggagcgcgc cggccaggtc cgggtggagg gcaaccggca gtacaacccc ggctggcacc
     3721 tggcccttga cctgcgctcc ctgctcaccg tctccgaatg cgtcgcgctg gcggcgctgg
     3781 agcgcaagga gagccgcggc ggccacaccc gcgacgacca cccgaaaccc gacccggagt
     3841 tcggcaggct caacatggcc ctgcgccaga aggaacccgg cggcgagatc gccctcgccc
     3901 gggagccgct gccggagatg cccgacgacc tgaagcaact cttcgacgaa gggaaggggt
     3961 agtcccagat gcccggcgac aacggcaacg gcactgccag tgccaacggc aacggctccg
     4021 ggggctacga cgtgacgctg cgcctgtggc gcggcgacga gagcggcggc gagctgcgcg
     4081 actacaccgt gccggtcgag gagggcgagg tcgtcctcga cgcggtccac cgggtgcagg
     4141 cgacccaggc cggcgacctg gccgtccgct ggaactgcaa ggccggcaag tgcggctcct
     4201 gctcggccga ggtgaacggc aagccgcgcc ttctgtgcat gacccggctg agcgtcttcg
     4261 agcgcggcga gccgatctcg atcacgccgc tgcggacctt cccgatcatc cgcgacctgg
     4321 tcaccgacgt gtcgttcaac tacgagatgg ccaagaaggt cccgccgttc cagcccaggc
     4381 cccgcgaggc ggacggcacc taccggatga tgcaggagga catcgaccgg gcgcaggagt
     4441 tccacaagtg catcgagtgc ttcctgtgcc agaacgtctg ccacgtgatc cgcgaccacg
     4501 aggacaacaa gcccgccttc gccggcccgc gcatgttcat ccgctacgcc gagctggaga
     4561 tgcacccgct cgacaccaac gaccgccgcg ccctgctccg cgagcagggg ggcctcggct
     4621 actgcaacat caccaagtgc tgcaccgagg tctgccccga ggagatcaag atcaccgaca
     4681 acgccatcat cccgctgaag gagcgggtgg tggaccgctc ctacgacccc ctcgcgcggc
     4741 tcctgaaacg cctcaaggga gacaaggcag actaaggatc aatcctgtca caccacctgt
     4801 acacgactgg gagcggctgc tagggtgatg agagagatca ttctctttcc cccctgggtc
     4861 tcgcacctca gcagctagaa ggacgtgacg acggtggccg tgccagaacc agtcctgcga
     4921 cgcgaacact tccgggacgc cgaggtccgc cagttccgcc cccgggccga ccccgctccg
     4981 gccccgggtc ccgatcccgg cgcgaacccg aagccccgcc acgtccggga cctcagcccc
     5041 gtccacgagg ccgtcaccac ccagctcctg gtggtctccg tcgccggcct ctccgccggc
     5101 gccgccgcga tccacttcgc catcgtccag tcccacttga aggagctgcc ggccgccggc
     5161 gtcttcttcc tcgccctggc cgtcgcccag gccctctggg cgatcgccat cccccgctac
     5221 cactcccgcc agatgctcgc ctggggcgcc gtcgccaacc tcgccatcgt cgccctctgg
     5281 ctctgggtcc gcgtctacgg cctccccgtc ggccccgaac cctgggtccc cgaagccttc
     5341 ggcatcaccg acaccatctc cgccaccttc gagctgaccc tcgccctcgc cgccaccgcc
     5401 ctcctcacca acctccgcct ccccctccgc cgcctgacgg tccccgcctc cctcctcgcc
     5461 ctcaccgtcg ccctcggcac ctggatcgcc ctcctccacg ccagcatgac ctgaccttgc
     5521 tgcttgccgc gccttggata tccggatatc ctgcggccat ggtgaagatc ctggtttcga
     5581 tcgacgacca gctcctgcag cggatcgatc gcgcggcgca gaggttgggg ctgacgcgca
     5641 gcgcctggct gtcccgcgta gcggcccgtg atgtggggga cgagcatggt cccggaaccg
     5701 accagcgggc acgtcgcgcc atggccgcgc tggaccagtt gttccagcgc caatcagttc
     5761 ctgaagacgc gaccgccgcg atccgcgccg accgggacac tcggtgaccg aggtcgtcct
     5821 tgacgcttcg gtcgttctca aatggttcac gcccggcgaa gagcccggcc ggtccgaggc
     5881 gagagcgctg cggcgagact acgacgccgg tcacctgacc gtcgttgtac cagggttgtt
     5941 tttcctggaa cttctcaacg tagccggccg ccgctgggga tgggacgggc cagctttgcg
     6001 agagcttgcc gacgacctcg acgacctcct cttcgacacc gccgagccag aactcccctc
     6061 cgtcgccacc tggatcgccc gcggcctcac cgcctacgac gccgcctacg tcgccctggc
     6121 cgaggagcgg agcatctcct tggtaaccgc cgacgacggc atcctcaaac tggcaggcac
     6181 catcgccagg ccactcgtcc cccactgact cgacaccccc tcaccgctgt cccctccgcc
     6241 gcggcacgaa ggcccccgct cggatctccc ctgctgagaa ctacttgatg ctgcagctca
     6301 ggtccgcggt tgtcgtcgaa gcagattgaa ctctgaccaa ccggtggcaa gtcagatcac
     6361 cgaggtggac gtagaggtca tagtctcctg gcgccaggct gaggtgatac aggttgccct
     6421 cgctgacctg ctgggtcgtg acgaggcggc cttgatgccg cacttcgacg gtgccgggct
     6481 catagtgggt gttgctcggg gcggcgctcg aagggccgcc gtggtagacg attccgcccg
     6541 tcaggacgcc gggggcagca cctcgcaacg ggctggttcc gcaggctgtc aggccgaaca
     6601 ggccgagcag cgtcaggcag atcacgcgcc accacatcat ggcctccaag tgaattgctc
     6661 cacgttggag gttggatgtt gggagcgtgc ggctggttcc cgccaggcag cggaattcag
     6721 ctggtaccag tggtgagcag gatggggcgg gcgtggctga ccaggtggat gccggtggcc
     6781 ttgatggcgg cgggccactg ggtggtgccg tcgccggagt cggcgaggac gagccagatg
     6841 cggccggttc cgccggaggc ttggatgcgg gcgagggcgg tgcggagcgc ggcttcgtcg
     6901 tcggcttcgg agttgttgcg gtgggccacc accacgtcgg gggtgttggg gtaggcgacc
     6961 ttgaagagga cggcggtgtt gaggtcggtg ggcgggaagc tcggctgctc cggccagtag
     7021 taggcgaagg ggaaggtggc gacccagccg accaccacga cgtcgccggg gcggcggtgg
     7081 gcgagcacgt cgtcgacctg ctggcgcatg cggcttgggg gcagcctgct gtgctgggcg
     7141 gtgtgggcgg cggggaccag cagcaccgcg gccaggccca ccgcgaccac gccgagcggg
     7201 gcggtcaaca gccgccgcca cgcccaggcg gcgaacgacc cgacggccag cgcggcgcag
     7261 gcggtcagca gcaccgcgta gaacatgctg gtgcgcacgt cgaacagggg gtagcggtgg
     7321 gcgatcccgg cgagcaactg cgcgaaggcg agcagcacca ccgacgtggc cacggctggg
     7381 cggcccgccc tccacaggac caccaggccc gcgaccacca gcgccgccga cagcagcgcc
     7441 ggcccgaacc cgatccggcc gagcagcagg tgcccctggc tggcgatgac atggacggcg
     7501 acgccgcgcc ccgacgtcag cggcacgaag aaccgccccc agtagcgctg catcccggcg
     7561 ttgttgccgg tgccgacgaa ggtgaggtag accgtgccct ccaccgccag cgcgcccgcg
     7621 ccgaccgccg ccagccagcc gagcttccgc cagtcccgac gcggcagcgc cagcaggccg
     7681 agcgcgccga acagcgccgc cgacacgaac acggtggtgt tgctcaggaa gatcgccggc
     7741 aggcacgcca cgcccaggac gaccacccgc cgggggctcc agcgctcctc gacccggctc
     7801 gccagccaga ccagcagcag ggcgacgaac gcctcggcgg tgtactgctt gaggctgcgc
     7861 atcatcagca tgcccggcgc gaccgcgacc gccagcccga ccgccacccc ttggatgcgc
     7921 ccgaactggc ggccgaacag ccagccggcc gctgccgcgg ccacggcgaa cagcatcggc
     7981 acgagccgca gccgctccgg aagcccgacg tgcgggacca ggcgcagcag cagctcccat
     8041 ccgaccggtg tggaggaggc cagcagcttg agctgcccga tcggcgcccg cagcgagtcg
     8101 gcgacccagc cctcgtcgag ccagaacgac cggctgagca tgtcccgtgg cagctccgcg
     8161 aaccaggcga ccagcaggat cggcgcgagc agcagcgcgt ccagccacca ccggtcccac
     8221 cagcgccggc cccggccccg gcgcccgggc ggctggtccg ggtcgcgatg cgggtccgcg
     8281 caccggtcag ccggcactct ggccggcacc ccagccccag ggggcccggc acggatcgcc
     8341 gcgtcaggtc ccgcggtctc cacgcccacg tccagccaac tctccttcag cgtccgcggt
     8401 gatggttgcc gcacgctcgg cggctcagcg gcgggtaatg cgtcctacca aagcttgccc
     8461 tatgtgatcg gaaaccactg cctaattccc cagtgaaaca atggtgaatg aggagcgaac
     8521 tatcgcatac gatgtcaaaa gcggcgcgag ccagacgcct gggcgccgat cgtcgatcga
     8581 tcgctggtcg tatacgattc agcaatgaat acgcagattc gcggcgccgt gcgccgtacc
     8641 gaagaccagc ggctggtcac tgggagcgcc aggtacaccg aggacctgga gatacctggg
     8701 gcgctccatg ccgcattcgt caggtcggcc gtcgcgcacg gccgcatcgt cgcgatcgac
     8761 accgaggagg ccaaggccgc acccggcgtg gccggggtgt acaccgccgc ggatctggaa
     8821 ctgggcaccc tc
//
LOCUS       DASWDC010000256         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_569999, whole genome shotgun sequence.
ACCESSION   DASWDC010000256 DASWDC010000000
VERSION     DASWDC010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569999"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VF995_08355"
     CDS             complement(<1..888)
                     /locus_tag="VF995_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEX9343608.1"
                     /translation="MAIKVALIGAGSARCYRELMDLCAFDGVGSYEVALHDPAPRRLA
                     VAGQLARRVAAATSAPCTITTHAERPPALDGADVVLTSISVGMQESIRPDFEIPARYG
                     LRQTVADTIGIGGIMRILRTAPAMAAIAADMRRYCPGGLLLNESNPLAMVGMALDRLE
                     PQITAVHLCHSPGRTAATLAGWLGVPPIELECEVAGVNHQAWVLRLAHHGEDLYPRLR
                     ELARKEEGPDGFASTVRADMLRRLGWFPTESSKHNAEYVPWYLPWDWEVERYGLQVNP
                     YWERRAHNVEGFASVADLAD"
     gene            complement(975..>1514)
                     /locus_tag="VF995_08360"
     CDS             complement(975..>1514)
                     /locus_tag="VF995_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX9343609.1"
                     /translation="RGDGRGLARRRGGRRWDGEGGGAGAGFARRLLAALRAGDQAGGD
                     RRGRQSAAVRVWRASSGYGGRLDLALDLRVDDHPDPVTELDRLLGLHQLYFGKPDPAS
                     LITLDPELASEGGGGLAGEVAAALALLGHDPASVGLEVALDRWAGIENLEERLVPGKL
                     DPRVLAILRQRAEAARRAC"
BASE COUNT          210 a          546 c          558 g          200 t
ORIGIN      
        1 gtcggccagg tcagcgacgc tggcgaaccc ctcgacgttg tgcgcccgcc gctcccagta
       61 ggggttgacc tgcaggccgt agcgctccac ctcccagtcc cagggcaggt accacgggac
      121 gtactcggcg ttgtgcttgg aggactcggt tgggaaccag ccgagccggc gcagcatgtc
      181 ggcccgcacg gtgctggcga acccgtcagg accctcctcc ttacgggcca gctcccgcag
      241 gcgcgggtag aggtcctcgc cgtggtgggc cagccgcagc acccaggcct ggtggttgac
      301 gccggcgacc tcgcattcca gctcgatcgg cgggacgccg agccagccgg ccagcgtcgc
      361 cgcggtgcgc cctggggagt ggcacaggtg gaccgccgtg atctgtggct ccaggcggtc
      421 cagtgccatg ccgaccatcg ccagcgggtt ggactcgttg agcagcagcc cgccggggca
      481 gtagcggcgc atgtcggcgg cgatggcggc catcgccggg gcggtccgca ggatccgcat
      541 gatcccgccg atgccgatgg tgtcggcgac ggtctgacgc agcccatagc gggcggggat
      601 ctcgaagtcc gggcggatcg actcctgcat gccgacgctg atcgaggtga gcaccacgtc
      661 cgcgccgtcg agggcgggag ggcgctcggc gtgcgtggtg atggtgcagg gtgcgctggt
      721 ggcggcggcg acgcggcggg cgagctgccc ggcgaccgcg aggcggcgcg gcgccgggtc
      781 gtggagggcg acctcgtagg agccgacgcc gtcgaaggcg cagaggtcca tcagttcgcg
      841 gtagcaccgg gcgctgcccg ccccgatcag tgcgaccttg atggccatcc caggagcgta
      901 gagaaggaat actctctaca gcaaggtcga acacctataa ttgcgcgctt ctgagcagat
      961 aaccgtaagc cgctctaaca cgcccggcgt gcggcctcgg cgcgttgccg caggatggcg
     1021 agcacccgcg ggtcgagctt gccgggcacc aggcgctcct ccaggttctc gatccccgcc
     1081 caacggtcga gcgcgacctc caggccgacg ctggccgggt cgtgaccgag gagggcgagc
     1141 gccgcggcga cctcgcccgc gagcccgccc ccgccctcgc tggcgagctc cgggtccagg
     1201 gtgatcaggc tcgccgggtc gggctttccg aaatacagct ggtgcaggcc gagcagtcgg
     1261 tcaagctcgg tgaccgggtc gggatggtcg tcgacgcgca ggtccagcgc gaggtcgagg
     1321 cggccgccat agcccgagct ggcgcgccac acccgcacgg cggccgactg gcgaccgcgc
     1381 cggtcgccgc cggcctgatc gccggcccgc agcgccgcca gcagccgccg ggcaaatcca
     1441 gcccccgccc ctcccccttc cccgtcccag cgtcgcccgc cgcgccggcg tgccaggccg
     1501 cgaccatcgc cgcg
//
LOCUS       DASWDC010000257         5192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1322839, whole genome shotgun sequence.
ACCESSION   DASWDC010000257 DASWDC010000000
VERSION     DASWDC010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322839"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1103
                     /locus_tag="VF995_08365"
     CDS             156..1103
                     /locus_tag="VF995_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9343610.1"
                     /translation="MALADGFGDNNWRRITSAEAKAEASLCPSVTKYLYTDGQGNTQK
                     AISDIQGLVAQGVNALVVFPDAGQAVLPAIRSAYKAGAVTVPYRVFPGGKDGVDYNYY
                     IKTDFTQAGVLWAQWMAKALNGKGNIANLGGPPANTQSLAEYQGMQSVFKNYPDIHFV
                     GETPYNVTNWDPAQTQRVVTALLAKYPKIDGITTDFGAALASSFGAFQQAGRKIPAVA
                     TEDSNLLSCDWQQMHASDPQFQLFTVDSQNWMVRTAVDFAIAKASGGQIPASTEVPQK
                     VFEDSITGKPHPVTCDKSLPQDALLSSHLSKAALAAALK"
     gene            1128..1790
                     /locus_tag="VF995_08370"
     CDS             1128..1790
                     /locus_tag="VF995_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9343611.1"
                     /translation="MTTSLLHLANIWKRFLGVEALKDVSLEAAGGEVHALLGENGAGK
                     STLMAIASGSLLPDSGAIEIAGEAIGALTPLLAHQLGVAIVHQDPALLPDLTVAENMA
                     IAVPKGLRASGAAKGAAKGAANGATAWMRRQLERVGCEVDLGLRVQELSVAQRQLVEI
                     AKALALDPRVLILDEPTAALGADMVERLFEQVRAVAASGAAVVYITHRLPEVRRTGAT
                     RA"
     gene            1790..4363
                     /locus_tag="VF995_08375"
     CDS             1790..4363
                     /locus_tag="VF995_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9343612.1"
                     /translation="MMTLSVRENAALVALPQFARFGVVQRRAEVDGVERQREALGIKA
                     PSLGTEISALSGGNQQKVVLARSLLAQPSLVLADEPTQGVDAAARVELYRILRGVADS
                     GIPVLIVSSDNLELEGLCDRIVVFSRGHVVGELAGAEVTEEKIAQAVVTATTHRREAA
                     GAARGRPTTAPGIRRRPRQRLRGWATGDYAPSVILALAIVALGAYTWSHNPRYLSPFN
                     VTSVLTLLAGLAFISLGQLCVILTGGIDISVGPLAGLLVVIASFFVNDGKPATTAALG
                     VLLMLATAVAVGLVNGCLVRFGRFTPVAATLTTYIALQGVSLLLRPFQGGLISTVVVD
                     RIETAIGAIPVAILAATALAAALEYCLRHSRWGLNLRAVGSRERAAHRLGVRVNRTVV
                     GAYVACSLLTFLGGIMLMAQIGIGDPTQGVTYTLASITVVVLGGTSLLGGRGSFIGAL
                     LGAALIQQILNATTFLHLSQAWQYWFQGIGVLLAAGLYTHARRSGVAPRGRGDPRAAA
                     GPGQARSRTSSHDGHYQRRHRDVKALVFEAPKRAVVADLEQPEIGPDEVLVRSRMVGI
                     CHSDFELYEGRYIIPISYPVIPGHEWAGEVVETGAAVVGPRPGDRVVGECVVGPGGRD
                     HFGFSINGANAEYLKVRAEWLHLLPDALSWTQGALVEPFSVAYNATVSAGGVDASDVV
                     AVLGGGPIGLLCTLAASASNAAVVLVEPRRHRRDKGLALGARLALDPTAGDFADAVAE
                     ATKGRGFDAVIEATGAPAAMASALELAGHGARIVYLGIDVGSTAPARLGLIQSKALRI
                     RGVIGSAGLWPQTIRFLASGVADPSRIVTACYPLGSALEALAAARDTAHNLKVHIQTA
                     P"
     gene            4395..>5192
                     /locus_tag="VF995_08380"
     CDS             4395..>5192
                     /locus_tag="VF995_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9343613.1"
                     /translation="MKAAVYHGPGDIRVESVPDPASPGPGEVVLRVTRAAICGTDAAE
                     WAHGPLLARPPVVLGHEFAGTVVAAGDGAGGLAAGDRVVCGAGVSCGGCAWCRAGRTN
                     LCASYLTLGLHADGGLAEFAVAPAAICQRVPERVTDDAAAMAQPLAVALHAVRRGGVE
                     AGQACVVIGVGGIGAFIVAGAAARGAAPLIAVDLDDDRLATASRLGAHQAVNLRGHDS
                     FDSSGGPAATMEPARGPSGHGTVPPEPPSAAAVAAAILDATGDQGAQV"
BASE COUNT          714 a         1916 c         1852 g          710 t
ORIGIN      
        1 gccggaagcg tcgtggcgag cgccatgggg gcgctgttcc tgagccagct cgatcaggtc
       61 gtccttacca ccggggtgaa cagcgccgtc cgcaacctga tcgaggcggc ggcgccggcg
      121 gcgggccgtc ctggtgcggc agcaagaaga tcacggtggc gctggcggat gggttcgggg
      181 acaacaactg gcgccggatc accagcgccg aggccaaggc cgaggccagc ctgtgcccga
      241 gcgtcaccaa atacctctac accgacgggc agggcaacac ccagaaggcc atctccgaca
      301 tccagggcct ggtcgcgcag ggcgtcaacg cgctggtcgt cttccccgac gccggccagg
      361 cggtcctgcc cgccatccgc agcgcctaca aggcgggcgc ggtcaccgtc ccctaccggg
      421 tcttccccgg cggcaaggac ggcgtcgact acaactacta catcaagacc gacttcaccc
      481 aggccggcgt cctgtgggca cagtggatgg ccaaggcgct gaacggcaag ggcaacatcg
      541 ccaacctcgg cggcccgccg gccaacaccc agagcctcgc cgagtatcag ggcatgcaga
      601 gcgtcttcaa gaactacccg gacatccact tcgtcggcga gacaccctac aacgtgacca
      661 actgggaccc cgcccagacg cagagggtcg tgaccgcgct gctggccaag taccccaaga
      721 tcgacggcat caccaccgac ttcggggcgg cgctggcgag ctccttcggc gcgttccagc
      781 aggccggccg caagatcccc gcggtggcga ccgaggactc caacctgctg tcctgcgact
      841 ggcagcagat gcacgcctcg gacccgcagt tccagctgtt cacggtggac tcgcagaact
      901 ggatggtgcg caccgcggtc gacttcgcca tcgccaaggc cagcggcggg cagatcccgg
      961 cctccaccga ggtgccccag aaggtcttcg aggactcgat caccggcaag ccgcacccgg
     1021 tgacctgcga caagagcctg ccccaggacg cgctgctctc cagccacctc tccaaggccg
     1081 cgctggcggc ggcgttgaag tagccgaggg gggatcgagg aatccgcatg acgacctcgc
     1141 tgctgcacct cgcgaacatc tggaagcggt tcctgggtgt cgaggcgctc aaggacgtct
     1201 ccctggaggc ggccggcggc gaggtccatg cgctcctcgg ggagaacggg gccggcaagt
     1261 cgacgctgat ggcgatcgcc tcgggctcgc tcctgcctga ctccggggcg atcgagatcg
     1321 ccggcgaggc gatcggggcg ctgacgccgc tgctggccca tcagctcggg gtggcgatcg
     1381 tgcaccagga cccggcgctg ctcccggacc tgacggtcgc cgagaacatg gcgatcgcgg
     1441 tcccgaaggg gctccgcgcc agcggagctg ccaagggagc tgccaaggga gctgccaacg
     1501 gcgccaccgc ctggatgcgg cgccagctcg agcgggtcgg ctgcgaggtg gacctgggcc
     1561 tgcgggtgca ggagctgagc gtcgcccagc ggcagctcgt cgagatcgcc aaggcgctgg
     1621 ccctggaccc gcgggtgctg atcctcgacg agccgaccgc ggcacttggc gccgacatgg
     1681 tcgagcgcct cttcgagcag gtccgtgccg tcgccgccag cggcgcggcg gtcgtctaca
     1741 tcacccaccg cctccccgag gtccggcgga ccggcgcaac gagggcctga tgatgaccct
     1801 gtcggtccgc gagaacgcgg cgctggtggc gctcccacag ttcgcccgct tcggcgtggt
     1861 ccagcgccgt gcggaggtcg acggcgtcga acgccagcgg gaggcgctcg gcatcaaggc
     1921 cccgtccctc ggcaccgaga tctccgccct gtccggcggc aaccagcaga aggtggtgct
     1981 ggcccggtcg ctgctcgccc agccgtcgct ggtgctcgcc gacgagccga cccagggcgt
     2041 ggacgcggct gcgcgcgtcg agctctaccg gatcctgcgc ggtgtggccg acagcggtat
     2101 cccggtgttg atcgtctcct cggacaacct cgaactggag gggctgtgcg accgcatcgt
     2161 ggtgttctcg cgcgggcacg tggtcggcga gctcgccggc gccgaggtca ccgaggagaa
     2221 gatcgcccag gcggtcgtga ccgccaccac ccatcgccgg gaggcggcag gggcggcccg
     2281 aggccggccc accaccgccc cgggcatccg gcggcgcccg cggcaacgcc ttcgcgggtg
     2341 ggccaccggc gactacgcgc ccagcgtgat cctcgcgctg gcgatcgtcg cgctgggcgc
     2401 ttacacctgg tcgcacaacc cccgctacct gagccccttc aacgtcacct cggtgctgac
     2461 gctgctggcg gggctcgcct tcatcagcct gggccagctc tgcgtgatcc tcaccggcgg
     2521 catcgacatc tccgtcggac ccttggcggg cctgctggtc gtcatcgcgt ccttcttcgt
     2581 caacgacggc aagcctgcca ccaccgccgc cctcggcgtc ctgctgatgc tggcgaccgc
     2641 ggtggccgtc ggcctggtga acggctgcct ggtgcggttc ggccgcttca cgccggtcgc
     2701 cgccacgctg accacctaca tcgccctcca aggagtctcg ttgctgctgc ggcccttcca
     2761 aggggggctc atcagcacgg tggtggtcga ccggatcgag acggcgatcg gcgcgatccc
     2821 ggtcgcgatc ctggccgcca ccgcgctcgc cgccgcgctc gagtactgcc tgcggcacag
     2881 ccgctggggc ctgaacctca gagcggtcgg ctcccgcgag cgggccgcgc accggctcgg
     2941 cgtccgggtc aaccgcaccg tggtcggtgc ctatgtggcc tgctcgctgc tcaccttcct
     3001 cggcgggatc atgctgatgg cacagatcgg catcggcgac cccacccagg gggtcaccta
     3061 tacgcttgcc agcatcaccg tggtggtgct gggcgggacc agcctgctcg ggggcagggg
     3121 gtcgttcatc ggggcgctgc tcggcgccgc gctcatccag cagatcctga acgcgaccac
     3181 cttcctgcac ctctcccagg cctggcaata ctggttccag ggcatcggcg tcctcctcgc
     3241 cgcggggctc tacacccacg ccaggcgcag cggcgtggcg ccgcgcggcc gcggggaccc
     3301 ccgcgcggcg gcaggtccag ggcaggcccg gagcaggacg agcagccacg acgggcacta
     3361 ccagcggagg catcgtgacg tgaaggcact ggtattcgag gctcccaaac gggcggtcgt
     3421 cgccgacctg gagcagccgg agatcggacc cgacgaggtg ctggtccgct cgcgcatggt
     3481 cgggatctgc cactccgact tcgagctcta cgagggtcgc tacatcatcc ccatctccta
     3541 cccggtcatc cccggccacg agtgggccgg ggaggtcgtc gagaccggcg ccgcggtcgt
     3601 gggcccgcgg cctggggacc gggtcgtggg cgagtgcgtg gtcggccccg gcggccgcga
     3661 ccacttcggc ttctcgatca acggcgccaa cgccgagtac ctcaaggtgc gggcggaatg
     3721 gctgcacctg ctgcccgacg cgctctcctg gacgcagggt gccctggtcg aaccgttctc
     3781 ggtcgcctac aacgcgacgg tgagcgcggg cggggtcgac gccagcgacg tcgtggcggt
     3841 gctcggcggc ggcccgatcg ggctgctgtg caccctggcc gccagcgcga gcaacgcggc
     3901 cgtggtgctg gtggagccgc ggcggcaccg gcgcgacaag ggactcgcgc tgggagcgcg
     3961 gctcgcgctc gacccgacgg ccggcgactt cgccgacgcc gtggccgagg cgaccaaggg
     4021 ccgcggcttc gacgccgtca tcgaggcgac cggcgccccg gcggcgatgg cctcggcctt
     4081 ggagttggcc ggccacgggg cacggatcgt ctacctcggc atcgacgtcg gctccaccgc
     4141 cccggcgcgg cttgggctga tccagtcgaa ggcgctgcgg atccgcgggg tgatcggctc
     4201 ggccgggctg tggccccaga cgatccgctt cctcgccagc ggcgtcgccg acccgagcag
     4261 gatcgtcacc gcgtgctacc cgctcggctc ggcgctggag gcgctggcgg ccgcgcggga
     4321 caccgcccac aacctcaagg tccacatcca gaccgcgcca tgacgcccag ggtgcccgta
     4381 ccgtccgtga aaccatgaag gccgccgtct accacggccc gggcgacatc cgggtcgagt
     4441 ccgtgccgga cccagccagt ccgggcccag gcgaggtcgt cctccgggtc acccgggcgg
     4501 cgatctgcgg caccgacgcc gccgagtggg cgcacggccc gctgctggcg cgcccgccgg
     4561 tggtgctcgg ccacgagttc gccggcacgg tcgtcgcggc cggggacggc gccggcgggc
     4621 tggcggccgg cgaccgcgtg gtctgcggcg ccggggtgtc ctgcggcggc tgcgcgtggt
     4681 gccgtgccgg gcggaccaac ctgtgcgcca gctacctcac cctcgggctg cacgccgacg
     4741 gcggcctcgc cgagttcgcc gtcgcgccgg cggcgatctg ccagcgagtg ccggagaggg
     4801 tgaccgatga cgccgcggcg atggcgcagc cgctcgccgt cgcgctgcac gccgtgcgcc
     4861 gcggcggggt cgaggcgggc caggcgtgcg tggtcatcgg cgtcggcggg atcggtgcct
     4921 tcatcgtcgc gggcgctgcc gcccgcggtg ccgcgccgct gatcgccgtc gacctggacg
     4981 acgaccggct ggcgaccgcc tcccgcctcg gcgcccacca ggcggtcaac ctgcgcggcc
     5041 acgactcttt tgattcatcg gggggccccg cggcaaccat ggaaccggcc cgcggtcctt
     5101 ccggccacgg gaccgtgccc cccgaacccc ccagcgcggc ggcggtcgcc gcggcgatcc
     5161 tcgacgccac cggcgaccag ggcgcccagg tc
//
LOCUS       DASWDC010000258         9656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_321749, whole genome shotgun sequence.
ACCESSION   DASWDC010000258 DASWDC010000000
VERSION     DASWDC010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321749"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1531
                     /locus_tag="VF995_08385"
     CDS             236..1531
                     /locus_tag="VF995_08385"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX9343614.1"
                     /translation="MEYAEAVRSLYARMPTRIGPSLERIQRLVELLDHPERSAPAIHL
                     TGTNGKTSSARMMTALLAAFGVGSGRYTSPHLQDVRERIALATRPISMEEFAETYTYL
                     EPYFAEVDREGEQPVTFHEALTALAFAWFAEVPVDAQVIEVGMGGSWDATNVVDGVVA
                     VLNRVSVDHPELGDTPVQVAVEKAGIIKPGATVVSQEQDPDVLAVISERTERLGGRLL
                     LAGRDFGLLRRRGAHGGQVLDLATPSGEVNGLFLPLYGRHQALNAASSLVAVQAFLGD
                     RALDGDVLREGFATVTSPGRLEVVGRNPLVLLDGAHNPEGARALAAALLEEFVVDRRT
                     IVLACLADKDLHGILEGLAPATGRLIVTSNSSPRTAGAEQLHKEAETVGLGAEVAPDV
                     ASAVGLALEGAGESEAVVVTGSLHTVGDARELLLGPGPA"
     gene            1579..1992
                     /gene="ndk"
                     /locus_tag="VF995_08390"
     CDS             1579..1992
                     /gene="ndk"
                     /locus_tag="VF995_08390"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9343615.1"
                     /translation="MADERTLVLCKPDAVARGLVGEVVGRLERKSLRLLAMELRTLDE
                     GVAKQHYGEHEGKPFFGELVSFITSGPLVAIAVEGPEAVAAVRALMGPTNPVSAPPGS
                     IRGDFCLEIGQNLVHGSDSAASAARELALFFPELR"
     gene            2251..3282
                     /locus_tag="VF995_08395"
     CDS             2251..3282
                     /locus_tag="VF995_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX9343616.1"
                     /translation="MQNAFRFLGRDMAVDLGTANTLVYVRGRGVVLNEPSVVAINTKT
                     NQVLAVGSDAKAMIGRTPGHIQAIRPLKDGVIADFDTTEKMLRYFVHKVHRRQILAKP
                     RVVVCVPSGITGVERRAVFNSAIQAGARSAFIIEEPMAAAIGVGLPVEDPTGNMVVDI
                     GGGTTEVAVVALGGIVTSQSIRIGGDELDEAIISYVKKEYSLMLGERTAETIKINIGS
                     AFPLAEEPTAEVRGRDLVSGLPKTIAVGAEEIRKAIEEPVNAIVDAVKNTLDRTPPEL
                     AADVMDKGIVLTGGGALLRGLDERLKHETSMPIHVADEPLHSVVYGSGKCIEQFETLK
                     RVLVSSGSR"
     gene            3373..4287
                     /gene="mreC"
                     /locus_tag="VF995_08400"
     CDS             3373..4287
                     /gene="mreC"
                     /locus_tag="VF995_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX9343617.1"
                     /translation="MYARPRRARMLLVLLLLASITVITIDFREGQSGPVAQLQRVSVA
                     AFGPLQRGVSAVVRPVGAFFSGIGQIGRLRADNRRLTAEVEQLQSEERTYQDAIGENQ
                     RLRGTLGMAARCGCKTVGALVVARSGSNFQWSVTIDAGSSRGIASGMPVLDADGLVGR
                     VSQVTAGYATVTLLDDPASGVAASLARTKGPGIVKGAAEAGLQFEPVQAGTDVRPGDE
                     VVTRGYQGGVFPAGLPIGMVARVDPAGAASLVPTVTLRPYANLGTIDVVAVVVSQPKA
                     PSRPRSGQRPQPQAGPAMAATSASGGGR"
     gene            4287..4799
                     /gene="mreD"
                     /locus_tag="VF995_08405"
     CDS             4287..4799
                     /gene="mreD"
                     /locus_tag="VF995_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX9343618.1"
                     /translation="MRRLLVLGAVIVAGLLLESTVLAAWRLAGARPDVLVIAVVAVAM
                     GAGPVEGASFGFCAGLIADLLFGQSQSVGVSALVYTAIGFAVGALRTYVTSSGAWVHL
                     ALAAAASLASVWLAGLVLRVFDQSSWGYVARTGLLAAAANLLLTPLVYPLVRRLVART
                     APAPTTVMFP"
     gene            4796..6778
                     /gene="mrdA"
                     /locus_tag="VF995_08410"
     CDS             4796..6778
                     /gene="mrdA"
                     /locus_tag="VF995_08410"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX9343619.1"
                     /translation="MKPKPPTRSQPPSHQRLSLLSVVVAVLLVALLSRLWYLQVLAAD
                     HYGDLAEGNRVRQVVVQAPRGRILDRKGRVLVSNRAAWAFTVKPAEMATKQGGVVLGR
                     LARLLGEPAAKLRQRMNDWNGSPLEGTPVAEDVPTDKLFYVTEHASQFPGVTPEVLAL
                     RDYPHGKLAAHVLGYVGEVSQGELKAPSFQQLRAGDVVGKAGVELTYDRWLRGSDGVQ
                     DLEVNAAGQVIRVLGGVAPKPGADLELSLDLDVQKTAEDSLAAAMRLARKLPDSQRGG
                     TYPAPASTAVVLDPNDGSVVALASLPEYDPRKFVGGISRKDYAAYNTNPNKPLLDRAV
                     QSAYPPGSTWKAVSALAGLQHGAITPQSTINCPGSFKFGNDLRHDWTPSGHGTVNLTR
                     SLQFSCDVYYYNLGAHFSNAEMSQERRHQQVDEQMQATARSLGFGRQPAIDLPYAVGG
                     TVPDRAWRKHVWQLLRPVYCKGKSALYQELCKSGSVWEGGDDLSIAIGQGSLQVSPLQ
                     LALGYGAIANGGTVYTAHVAQAVLDPVTHKPVRTITPKINSVARIPAGDFAAVTEGLK
                     AVPAAGTAVAAFAGFPLDRYPVGGKTGTADLPPKAPFAWFASFAPIGSPRYVIVAMVE
                     QAGHGGESAAPVAKVLYQKLFGLPVQPIAAGADRSG"
     gene            6784..7974
                     /gene="rodA"
                     /locus_tag="VF995_08415"
     CDS             6784..7974
                     /gene="rodA"
                     /locus_tag="VF995_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX9343620.1"
                     /translation="MQESWGSWTDRVSRLGWEAASERPMTWRAPWRHLDPTVALAAVG
                     LAVFGLVAIYSSTFASLREHGLPGAMIMQKQLVNLLLGVVAMALAMLVDYRRLAGWAM
                     VCYGFVVVILAVVLTPLGATSNGAQSWFAVGPYQIQPSEYAKIGVILVLATILAGTRE
                     RAGPKRIAFALAALGLVAGEILLQPDFGTMMVFVAILFGILLVSGAQYRWLLILLLVG
                     SVGVVGMFKLNVLKQYQKERLTAFIDPSADATGRGFTYNARQALIAIGSGGVTGKGYL
                     RGSQTNLQYVPEQRTDFIFTVIGEEFGFAGSAALLGLLALLLWRGLRIAALARDRFGA
                     LIACGIVAMLVFQTFINIGMDIGIMPITGIPLPFVSYGGSSLISSFIAIGLLESIHMR
                     RFAR"
     gene            8945..>9656
                     /locus_tag="VF995_08420"
     CDS             8945..>9656
                     /locus_tag="VF995_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343621.1"
                     /translation="MATEQVAEAEAESATPARAGRSRRRRGRGGRSARPEVGDGDGDA
                     AAAAVPPAALTGQDPEATAAVAAGVAAASSDDGDAAEAAEEDEAEDESSAAGSARTRA
                     RRRRRAAAKARIRTDEPATDGEPATEPASPNGAAATSPVLAADDGEGEGEATATQQAA
                     APATSAPAPAATGERAPAPSAAGPGRSGGRGRQRTSPQKAPTAAASTPAGAAETEPEA
                     AAAAAAGTSAAPAAEQRQP"
BASE COUNT         1346 a         3512 c         3440 g         1358 t
ORIGIN      
        1 gacttcgtcg ccaaggcacc cctgccggtg gtcggcaagg cccgcgcccg cctcacggag
       61 gtcgaagccg acctggtcaa ggtcgccgag cagctcgccg ccctcggcgc tgcgcgtccc
      121 tgagcgggcg ccccgggcca ctacaatcgc caggcactca agcctggctc ttgagcctgg
      181 ctcttgagcc tggctcgcgc cgggaaccct ggatcgttcg aggagaagag cgctggtgga
      241 gtacgcggag gctgtcaggt cgctgtacgc acggatgccg acgcggatcg ggccgagcct
      301 tgagcggatc cagcggctgg tggagctgct ggaccatccg gagcggagcg cgccggcgat
      361 ccatctgacc gggaccaacg ggaagaccag cagcgcgcgc atgatgacgg cgctgctggc
      421 ggcgttcggg gtgggatcgg gacgctacac cagcccacac ctgcaggacg tgcgggaacg
      481 gatcgcgctg gcgacgcggc cgatctcgat ggaagagttc gcggagacct acacctatct
      541 ggagccgtac ttcgcggagg tggaccggga gggggagcag ccagtcacct tccatgaggc
      601 gctgaccgcg ctggcgttcg cctggttcgc ggaggtgccg gtggacgcgc aggtgatcga
      661 ggtcggcatg ggcggctcct gggacgccac caacgtggtc gacggggtgg tcgcggtcct
      721 caaccgggtc tcggtcgacc atcccgagct gggggacact ccggtccagg tcgcggtcga
      781 gaaggcgggg atcatcaagc cgggcgcgac cgtggtcagc caggagcagg atcccgacgt
      841 gctggcggtg atcagcgagc ggaccgagcg gctcggcggc cggctgctgc tcgccgggcg
      901 cgacttcggc ctgctgcggc gccggggcgc ccacggcggg caggtgctcg acctggccac
      961 gcccagcggg gaggtcaacg gcctgttcct gccgctctac gggcggcacc aggcgctgaa
     1021 cgccgccagc tcactggtgg cggtgcaggc gttcctcggc gaccgggcgc ttgacgggga
     1081 cgtcctccgg gaggggttcg ccaccgtcac ctccccgggg cggctggagg tggtggggcg
     1141 caacccgctc gtgctgcttg acggcgccca caaccccgag ggcgcccgtg ccctggccgc
     1201 cgcgctgctg gaggagttcg tggtggaccg gcgcaccatc gtgctggcct gcctggccga
     1261 caaggacctg cacggcatcc tcgagggcct cgcgccggcg accgggcggc tgatcgtcac
     1321 cagcaactcg agcccgcgaa ccgccggggc cgagcagctc cacaaggagg ccgagacggt
     1381 cggcctcggc gccgaggtcg ccccggacgt ggccagcgcg gtgggtctgg cgctggaggg
     1441 cgccggcgag tccgaggcgg tggtggtgac cggctccctc cacaccgtcg gcgacgcccg
     1501 cgagctgctg ctggggccgg gacccgcgta agatccgcga gcatcgctcg acattcaccg
     1561 agtctgggag gttggcgcgt ggccgacgaa cgcaccctgg tcctctgcaa gcccgacgcg
     1621 gtcgcccgcg gcctggtcgg ggaggtcgtc ggccggctgg agcgcaagag cctgcggctg
     1681 ctcgccatgg agctgcgcac cctggacgag ggcgtggcca agcagcacta cggcgagcac
     1741 gagggcaagc cgttcttcgg cgagctggtc tccttcatca ccagcggccc gttggtggcg
     1801 atcgcggtcg aagggccgga ggcggtcgcg gcggtccgcg ccctgatggg cccgaccaac
     1861 ccggtcagcg ccccgccagg ctcgatccgc ggcgatttct gcctggagat cggccagaat
     1921 ctggtccacg gctccgactc ggccgcctcc gccgcccggg agctggcgct gttcttcccc
     1981 gagctgcggt agcgccccgc cgcggtcacc ccggccgtcc tctcgctggc gcattcgccg
     2041 gtcaagtcca gcgggcgacc cgccgaaggg atcgagatgg cggttcggta acgtctcgct
     2101 gagcctcccg gagacgaacg accggctggt gtaccctgac gaagttccca gtggctgacc
     2161 gctcacgcag ctcaacacgg ctgccctggc actttttggc gccccggccg aatcggggtc
     2221 tgcaaaatgc gctcgaaggg gctggttcct ttgcagaatg cctttcgttt tctcggtcgg
     2281 gacatggcgg tcgatcttgg gaccgccaat acgctcgtgt acgtgcgcgg gcgcggcgtg
     2341 gtcctgaacg agccgtcggt ggtagcgatc aacaccaaga ccaaccaggt gctcgccgtc
     2401 ggatcggacg ccaaggcgat gatcggccgc acacccggcc acatccaggc gatccggccg
     2461 ctgaaggacg gcgtcatcgc cgacttcgac accaccgaga agatgctgcg ctacttcgtc
     2521 cacaaggtgc accgccgcca gatcctcgcc aagccgcggg tggtggtgtg cgtcccgtcc
     2581 gggatcaccg gagtggagcg gcgcgcggtc ttcaactctg ccatccaggc gggcgcgcgc
     2641 tccgccttca tcatcgagga gccgatggcc gccgcgatcg gcgtcggcct gccggtcgag
     2701 gaccccaccg gcaacatggt ggtcgacatc ggcggcggca ccaccgaggt cgccgttgtg
     2761 gctctcggcg gcatcgtgac cagccagtcg atccgcatcg gcggcgacga gctggacgag
     2821 gcgatcatct cctacgtgaa gaaggagtac tcgctgatgc tcggcgagcg gaccgccgag
     2881 acgatcaaga tcaacatcgg ctccgccttc cccctcgctg aggagccgac cgccgaggtg
     2941 cgcggccgtg acctggtcag cgggctgccc aagacgatcg ccgttggcgc cgaggagatc
     3001 cgcaaggcga tcgaggagcc agtcaacgcg atcgtcgacg cggtcaagaa caccctcgac
     3061 cggaccccgc cagagctcgc cgccgacgtg atggacaagg gcatcgtgct caccgggggc
     3121 ggcgcgttgc tgcgcgggct cgacgagcgg ctcaagcacg agaccagcat gccgatccac
     3181 gttgccgacg agccgttgca ctcggtggtg tacggctccg gcaagtgcat cgaacaattc
     3241 gagaccctga aacgggtgct ggtcagttcc ggatcacgct gatctgggca agtgatctgg
     3301 aagcgtcaga ccgcgggaag gcgttgttcg tttcgttccg gaagctggag cttgagctgg
     3361 aggttgggtc ggttgtacgc acgcccccgc cgcgcccgca tgctgctggt gctgctgctg
     3421 ctcgcctcga tcacggtgat caccatcgac ttccgcgaag gccagagcgg accggtcgcg
     3481 cagctccagc gagtctcggt cgcggccttc gggccgctgc agcgaggggt cagcgcggtg
     3541 gtgcggcccg tcggtgcctt tttcagcggg atcggtcaga tcggacgcct gcgggctgac
     3601 aaccgccgcc tcaccgccga ggtcgagcag ctccaaagcg aggaacgcac ctaccaggac
     3661 gcgatcggcg agaaccagcg cctgcgcggc accctcggga tggccgcgcg atgcggctgc
     3721 aagacggtcg gggccctggt ggtggcccgc tccggctcga acttccagtg gtcggtgacg
     3781 atcgacgcgg gttccagccg ggggatcgcc agcggcatgc cggtgctcga cgccgacggc
     3841 ctggtcggcc gtgtcagcca ggtgaccgct ggctacgcca ccgtgacgct gctcgacgac
     3901 cccgccagcg gcgtcgcggc cagtcttgcc cgcaccaagg gccccggcat cgtcaagggc
     3961 gccgccgaag cgggcctgca gttcgagccg gtgcaggccg gcaccgacgt gcgcccgggc
     4021 gacgaggtcg tcacccgcgg ctaccagggc ggcgtcttcc cggcggggct gccgatcggg
     4081 atggtcgccc gggtcgaccc cgccggtgcc gccagcctgg tcccaaccgt gacgctgcgt
     4141 ccctatgcca acctcggcac catcgacgtg gtcgccgtgg tggtctccca gccgaaggct
     4201 ccgtcgcgcc cgcgatccgg ccagcggccg cagccccagg cagggccggc gatggcggcc
     4261 acctcggcct cgggtggtgg ccgctgatgc gccggctcct ggtcctcggc gcggtgatcg
     4321 tcgccgggct gctgctcgaa tccaccgtgc tcgccgcctg gcggctggcc ggagcgcggc
     4381 cggacgtgct ggtgatcgcc gtggtggcgg tcgccatggg cgccgggccg gtggaggggg
     4441 cgagcttcgg gttctgcgcc gggctgatcg ccgacctgct cttcggccag agccagtcgg
     4501 taggggtgtc ggcactggtc tacacggcga tcgggttcgc ggtcggtgcg ctgcggacct
     4561 atgtgaccag ctcgggcgcc tgggtgcacc tggccctggc cgccgcagcc agcctcgcct
     4621 cggtatggct ggccgggctg gtactgcgcg tcttcgacca gtcgtcctgg ggctacgtgg
     4681 cccgcaccgg gctgctggcc gccgccgcca acctgctgct caccccactg gtctacccgc
     4741 tggtccggcg tctcgtcgcc cggaccgccc cggcgccgac cacggtgatg ttcccgtgaa
     4801 accgaaaccg cccacgcgct cccagccacc gtcgcaccag cggctgtcgc tgctgtcggt
     4861 ggtcgtcgcc gtcctgctgg tcgccctgct gtcgcggctg tggtacctgc aggtgctggc
     4921 cgccgaccac tacggcgacc tggccgaggg caaccgggtg cggcaggtgg tggtgcaggc
     4981 gccacgcggg cgcatcctcg accgcaaggg ccgcgtgctg gtcagcaacc gggctgcctg
     5041 ggccttcacc gtcaagccgg ccgagatggc caccaagcag gggggcgtgg tgctcgggcg
     5101 cctcgccagg ctgctcggcg agccggccgc caagctgcgg cagcgcatga acgactggaa
     5161 cggctcgccg ctggagggca cccccgtcgc cgaggacgtg cccaccgaca agctcttcta
     5221 cgtcaccgag cacgccagcc agttccccgg cgtgaccccg gaggtgctgg cgctgcgcga
     5281 ctacccgcac ggcaagctcg cagcgcacgt gctcggctac gtcggggagg tctcccaggg
     5341 cgagctgaag gcccccagct tccagcagct gcgggcgggc gacgtggtcg gcaaagccgg
     5401 ggtggagctg acctacgacc gctggctgcg cggttccgac ggcgtccagg acctggaggt
     5461 gaacgccgcc ggccaggtca tccgcgtgct cggcggggtc gcgccgaaac ccggcgcgga
     5521 cctggagctg tcgctcgacc tggacgtgca gaagaccgcc gaggactccc tcgccgccgc
     5581 gatgcggctg gcccgcaagc tccccgacag ccagcgcggc ggcacctacc cggcgcccgc
     5641 gtcgaccgcg gtggtgctcg accccaacga cggctcggtg gtcgccctgg ccagcctgcc
     5701 cgaatacgac ccgcgcaagt tcgtcggcgg catctcgcgc aaggactacg ccgcctacaa
     5761 caccaacccg aacaagccgc tgctggaccg tgccgtccag tcggcctacc cgcccggctc
     5821 gacctggaag gcggtcagcg ccctcgccgg gctgcagcac ggcgcgatca ccccgcagag
     5881 caccatcaac tgccccggct cattcaagtt cggcaacgac ctgcgccacg actggacccc
     5941 gagcgggcac ggcaccgtca acctgaccag gagtctgcag ttctcctgcg acgtgtacta
     6001 ctacaacctc ggcgcccact tctccaacgc cgagatgagc caggagcgcc gccaccagca
     6061 ggtcgacgag cagatgcagg cgaccgcccg aagcctcggc ttcggccgcc agcccgccat
     6121 cgacctgccc tacgccgtcg gcggcacggt gccggaccga gcctggcgca agcacgtctg
     6181 gcagctgctc aggcccgtct actgcaaggg caagagcgcc ctgtaccagg agctgtgcaa
     6241 gagcggctcc gtctgggagg gcggcgacga cctgagcatc gccatcgggc agggctccct
     6301 gcaggtctcg ccgctgcaac tggccctcgg ctacggcgcg atcgccaacg gcggcacggt
     6361 ctacaccgcc cacgtggcgc aggcggtgct cgacccagtc acccataaac cggtccggac
     6421 gatcaccccg aagatcaact cggtggcacg catccccgcc ggcgacttcg ccgcggtcac
     6481 cgagggcctc aaggccgtcc cggcggccgg gaccgccgtc gcggccttcg ccggcttccc
     6541 gctcgaccgc taccccgtcg gcggcaagac cggcaccgcc gacctgccgc cgaaagcgcc
     6601 gttcgcctgg ttcgcctcct tcgccccgat cggtagccct cgctacgtca tcgtcgccat
     6661 ggtcgaacag gccggccacg gcggcgagtc ggcggccccg gtcgccaagg tgctgtacca
     6721 gaagctgttc ggcctccccg tccaaccgat cgccgctggc gccgatcgga gcggttgagt
     6781 cctgtgcaag agtcctgggg atcgtggacc gaccgggtct cgcggctcgg ctgggaggcc
     6841 gcgagcgagc ggccgatgac ctggcgcgcg ccctggcggc acctggaccc gacggtggcg
     6901 ctggccgccg tcgggctggc ggtgttcggc ctggtggcga tctactcgtc gaccttcgcg
     6961 agcctgcgcg agcacgggct gcccggggcg atgatcatgc agaagcagct ggtcaacctg
     7021 ctgctcggcg tggtcgccat ggccctggcg atgctggtcg actaccgccg cctcgccggg
     7081 tgggcgatgg tctgctacgg cttcgtggtg gtgatcctgg cggtggtgct gacgccgctc
     7141 ggggcgacct ccaacggcgc acagtcgtgg ttcgcggtgg gcccctacca gatccagccg
     7201 tccgagtacg ccaagatcgg ggtgatattg gtcctcgcca cgatcctggc cggtacgcgc
     7261 gagcgggccg ggccgaagcg gatcgccttc gcgctcgccg ccctcgggct ggtcgcgggg
     7321 gagatcctgc tgcaaccgga cttcggcacg atgatggtgt tcgtcgcgat cctgttcggg
     7381 atcctgctgg tcagcggggc ccagtaccgc tggctgctga tcctgctgct ggttggcagc
     7441 gtcggcgtgg tcgggatgtt caagctgaac gtgctcaagc agtaccagaa ggagcggctc
     7501 accgccttca tcgacccgag cgcggacgcc accggccgcg gcttcaccta caacgccagg
     7561 caggcgctga tcgcgatcgg gtcaggcggg gtgaccggaa agggctacct gcggggcagc
     7621 cagaccaacc tccagtacgt gccggagcag cgcaccgact tcatcttcac cgtgatcggg
     7681 gaggagttcg gcttcgccgg gtcggcggcc ctgctgggcc ttctggcgct gctgctgtgg
     7741 cgcgggctgc gcatcgccgc gctggccagg gaccggttcg gcgcgctgat cgcctgcggg
     7801 atcgtggcga tgctggtgtt ccagacgttc atcaacatcg gcatggacat cggcatcatg
     7861 ccgatcaccg gcatcccgct gccgttcgtc tcgtacggtg gttcgtcgct gatctccagc
     7921 ttcatcgcca tcgggctgct tgagagcatc cacatgcgcc ggttcgcccg ttagcgctga
     7981 atgtgtcggg ggactagtcc ttctcggcaa ctccccttag caaccccggc cccttggctg
     8041 tagactcaac tcagttttgc gccatgccga tcctgcggtg cggcccgacg acgcggtgac
     8101 cgccttcgca ctggctctcg ggcgccaacc atgcgtccag cccctcccgg gcagcccggc
     8161 ctagtcagcg cggaggcgcc agccgtgtgg atcgtggccg tcgcaacagg cgacgggcca
     8221 gcaccctcgg gcggggatcc ttgatcctcg tcgacgcccg acgggtccgg atgtgtatgg
     8281 cggcgccgtc cgacccggac ggagacttcg agtcaccggc tgacgctggg gaccaggttc
     8341 cgaccggttc ggccggggag cggtcaccgc acgtcccgtc ccccgggtgc gcaagagatt
     8401 tcggccgccc ccgtcggcga ccgcccgaac ttggtgagga gcattcatgg actccccgga
     8461 agagtcccgg gtcgaaggta cccgggacct ccctggcgca cccgctgaca gcggcgcgcc
     8521 caatcccgac cccatccccg attcccagat ccccgattcc cagccctcgc ggcccacatc
     8581 cgctgacgga gcgcgtgatg gcgcgccagc gggcgatgcc gacccggcag ccgagggcgc
     8641 cccggccgcc gctccggcct cggtgacccc cgcccggcgg cgcccacgcg ccgctgcctc
     8701 gttccccggc gggccgcctc cctccgcttc agcgaccccc gcggcgacac tcccggcccc
     8761 ggcgccgccc acccccacac cacctgccga gccggcgccg agcttccggg tggtcacggt
     8821 ggagagcact gccgcctccc gcccggcggg cccgccccca gctccggctc cctccgccgc
     8881 tcagcctgtc cagccgccac cggaggcgga gccggcccga gcggagcagg aagcgcccga
     8941 gcaggtggct accgagcagg tggccgaggc tgaggctgaa agcgccacac cggcccgggc
     9001 cgggcggagc cgccggcgcc gtggcagggg cggccgttcg gcccggccgg aggtcggcga
     9061 cggcgacggc gacgcggcgg ctgcggccgt cccgccggcc gccctgacag gccaggatcc
     9121 cgaagccacg gccgcggttg cggccggtgt cgccgctgcc tccagcgacg acggcgacgc
     9181 tgccgaagcg gccgaggaag acgaagcgga ggacgagtcg tcggccgccg gctcggcgcg
     9241 gacccgggcg cggcgccggc gccgcgccgc ggcgaaggcc cgcatccgca ctgacgagcc
     9301 cgccaccgat ggcgagccgg ccaccgaacc ggcctcgccc aacggcgcgg cggcgacgtc
     9361 gccggtgctg gccgcggacg acggcgaggg cgagggcgag gcgaccgcga cccagcaggc
     9421 cgccgctccg gcgacctcgg cgccggcccc agccgccacc ggtgaacggg ccccggcgcc
     9481 atccgcggcc ggcccgggcc gctcgggtgg ccgtggccgg cagcggacct cgccccagaa
     9541 ggcgcccacc gccgccgcct ccacacccgc gggggccgcc gagacggaac ccgaagccgc
     9601 cgctgccgcc gctgccggaa ccagcgccgc gccggccgcc gagcaaaggc agccga
//
LOCUS       DASWDC010000259         8348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1489810, whole genome shotgun sequence.
ACCESSION   DASWDC010000259 DASWDC010000000
VERSION     DASWDC010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489810"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VF995_08425"
     CDS             <1..531
                     /locus_tag="VF995_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9343622.1"
                     /translation="PGLVVVDEAYGEFGGSHAATLLPSHPNLVVTRTFSKAFRMAGAR
                     LGYLLGDPAVVDGIRLVRLPYHLSALIQTAGLRALAHVGTLMDHIVATRRERDRVAAG
                     LVALPGVGVLPSDANFLCFSTGVSPRVLWRRLLDRGVLVRDVSGYPTLDRYLRVTIGT
                     PAENDAFLAALAAELA"
     gene            531..1148
                     /gene="hisB"
                     /locus_tag="VF995_08430"
     CDS             531..1148
                     /gene="hisB"
                     /locus_tag="VF995_08430"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX9343623.1"
                     /translation="MAAPAPPRAPRRARVERRTSEVAVTVELDLDGSGQASADTGVPF
                     LDHMLAQLGKHARFDLAIVAKGDLQVDAHHTVEDTGIALGQAFGEALGDKSGVRRFGD
                     ATVPLDEARARCAVDLSGRPYLRWDVPVASTPGELGGFDPRQARHLLEGLVANARCTV
                     HLLLEAGDLPHHVLEACFKALARALGDACAPDPRAGSGSTKGTLA"
     gene            1145..2011
                     /locus_tag="VF995_08435"
     CDS             1145..2011
                     /locus_tag="VF995_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX9343624.1"
                     /translation="MNPNRSRPRARPPVALLDYGVGNLHSAAKALDRAGAEVRTVPTV
                     AAAEAAGAWAMVVPGVGAYRACLEGLGRAGGAAAVAAWIDAGRPLLGICVGMQLLFEA
                     SEEGPVGDGIGALAGTVRRLGGFGGHGPAAGSTEAVSQRGRGAPRWVGGPVKVPHIGW
                     DEVTVQPGGSGGTVPRPEGVRADSTAAAGPPDGSRSRLFAGIADGARFYFVHSYAPEA
                     SGAAVAAVCDYGSPFAAAVERGNLFGTQFHPEKSSAAGHALLSNFVALVAEWGAGDQE
                     PEGARGAVRGIS"
     gene            2080..2835
                     /locus_tag="VF995_08440"
     CDS             2080..2835
                     /locus_tag="VF995_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX9343625.1"
                     /translation="MPLTLLPAVDLLGGQAARLVQGALGTETAYGDPVEAARSFAAQG
                     AEWLHVVDLDAAFGRGPRNRAQIGRIVAAVAPVRVEASGGVRHPDDLDALLGLGAARV
                     VIGTAALEDPAWVGEACERHGDRVAVGLDARGRELRARGWTTGGGDLFEVLDRLDRAG
                     CRRFVYTEVAADGMLSGPALDGLRAVLAATKRPVVASGGVATLDDLRDLAALASGGLE
                     GVIVGKALYAGRFTVAEALATVRAAAGIGEARP"
     gene            2832..3623
                     /gene="hisF"
                     /locus_tag="VF995_08445"
     CDS             2832..3623
                     /gene="hisF"
                     /locus_tag="VF995_08445"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX9343626.1"
                     /translation="MTLARRVIPCLDVTAGRVVKGVSFVNLVDAGDPVELAALYDAEG
                     ADELVFLDITATVEARETLFDVVARTAEQVFIPLTVGGGVRTPDDVRRLLRAGADKVS
                     VNSAAVRDPDLLSAGADTYGVQCMVLAIDARRRPGARPGARPSGGGTGWEVVVDGGRT
                     STGLDALAWAEEGAARGAGELLVTSMDADGQQTGYDLDLVEAVTAAVDIPVIASGGAG
                     NPDHFTAACLAGADAVLAASVFHFGQLSISQVKDSLAAAGLPVRT"
     gene            complement(3633..8345)
                     /locus_tag="VF995_08450"
     CDS             complement(3633..8345)
                     /locus_tag="VF995_08450"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX9343627.1"
                     /translation="MVTDDMPFLLDSVTMELNRHGLTIHLPIHPTLVVRRDAAGELLQ
                     LLPPEGASKESPELPTDALREACIHIEVDRQTEPQLLTQLRAGIGRVLGDVRVAVEDW
                     APMRERVAGILAELGEHPPPVEAAELAETRALLEWIDDGNFTFLGYRAYDLDVVDGEN
                     VLRPVPGSGLGILRGHSTRPKGKGKAATPGPAVARSHSRSFAKLPPQARRQARDRRPL
                     VLTKANSRATVHRPSYLDYVGVKRFDEQGAVIGEHRFLGLYTSAAYNRNPREIPALRR
                     KVATVLELAGLPPGGHDWKALLNILETFPRDELFQIADEQLFTVAMGILRLEERRRVR
                     LFLWKERYERFVSALVYVPRDRYTTATRTRIEAVLRHAFPGSTLDFAVLLSESVLARL
                     HFVIRVPDGEIPPYDQHELEAKLAETTRSWTDELQALLLGQHGEEAGNRLSARYAEAF
                     PAAYREDQSARSAVYDIQRMEDLTAGARDAQNAMSLYRPLEAPLGSLRLKLFSRERPL
                     PLSDVVPMLEDMGVEVVEERPYEIRPPAAEAEAGMTSVWLHDFGLQYAPGAPGVQPAG
                     AGATGDAVMAEVDLGGVRELFQDAFGRILSGEAESDGFNALVLGAELSWREIVLLRAC
                     CKYLRQTRLTFSQAYMEEALCANPAVARQFVELFSARFDPARQRASDGDTAEVEALRE
                     RIGTAIDSVASLDTDRILRSFLALLEAMTRTNYFQVQADGAPKPYLSFKLDPERVPSL
                     PLPRPMFEVFVYSPRTEAVHLRGGRVARGGIRWSDRREDFRTEILGLMKAQMVKNAVI
                     VPTGAKGGFVVKCLPPPADREALGAEVAACYELFMRGLLDLTDNRDHDGQVMPPAGVV
                     RYDEDDPYLVVAADKGTATFSDLANSIAREYGFWLDDAFASGGSAGYDHKKLGITARG
                     AWESVKAHFRALNMNPQTTDFTVVGIGDMSGDVFGNGMLRSRHSKLVGAFNHLHIFLD
                     PHPDPERGFAERERLFTLPRSSWSDYDPAAISKGGGVWPLTAKSIPLSAEAREVLGLE
                     AGVQALTPSELISALLRAPVDLLWNGGIGTYVKASSESHAAARDRGNDAVRVDGAELR
                     CRVVGEGGNLGFTQLGRVEYALGGGRINTDFIDNSGGVDCSDHEVNIKILLNQVVADG
                     DMTRKQRDRLLAQMADEVAQVVVHDNYRQAQALSITESRAAASLDDHLRLIRSLERAG
                     RLNRRLEGLPSDEELDARRAAGHGLTRPELAVLLAYGKVGVKEELLASDVPEDPYLSD
                     DLERYLPAKLREPFRDQVRRHPLRREIIATHITNSMVNRVGTTFVHRLREDTGTHAPD
                     IARAYTVAREVFDARRLWKELGQLDDQVDASVQTALMAEGRRLIERAARWFLRNRRQP
                     LDIAATVSSFAPGITALAQELPKLLAPADSESLDHAAERFVALDVPPDLANRVASMDA
                     LFSGLNVIEVASAAGETVEAVAAAYFALGARLDLHWLRDQIAKLPTVTHWQALAQGAL
                     RDDLYSEQRELTAQVLRPGVEDRDAQQLVNAWVDVNRTAVDRCLALFDDLKEVDAVDI
                     AMVSVALREIRNLSDTGD"
BASE COUNT         1056 a         3021 c         3087 g         1184 t
ORIGIN      
        1 cccggcctgg tggtggtcga cgaggcctac ggcgagttcg gcggctccca cgcggccacg
       61 ctgctgccga gccacccgaa cctggtggtc acccggacct tctccaaggc gttccggatg
      121 gccggggcgc gcctcggcta cctgctcggc gacccggccg tggtcgacgg gatccggctg
      181 gtgcgcctgc cctaccacct ttcggcgctc atccagacag ccgggctgcg cgccctcgcc
      241 cacgtcggca cgctgatgga ccatatcgtg gcgaccaggc gggagcgcga ccgggttgcg
      301 gccgggctcg tcgccctgcc gggggtcggg gtgctgccca gcgacgcgaa ctttctctgc
      361 ttctcgaccg gcgtgtcgcc gcgggtgctg tggcggcgac tgctcgaccg gggggtcctg
      421 gtccgcgacg tgtcgggcta cccgacgctg gaccgctacc tgcgggtgac catcggcacc
      481 ccggccgaga acgacgcgtt cctggccgcg ctcgccgcgg agctggcctg atggccgcgc
      541 cagctccgcc gcgtgcgccc cgccgcgccc gggtcgagcg gcgcaccagc gaggtcgcgg
      601 tcactgtcga gctggacctc gacggcagcg ggcaggccag cgccgacacc ggcgtgccgt
      661 ttctcgacca catgctcgcc cagctcggca agcacgcccg cttcgacctc gccatcgtcg
      721 ccaaaggcga cctgcaggtc gacgcccacc acaccgtcga ggacaccggc atcgcgctcg
      781 gccaggcgtt cggcgaggcg ctcggcgaca agtcgggggt gcgccgcttc ggcgacgcca
      841 ccgtccccct cgacgaggcc agggcgcgct gcgccgtcga cctctccggc cgcccctatc
      901 tgcgctggga cgtcccggtg gccagcaccc cgggtgagct cggcggcttt gacccgcgcc
      961 aggcccgcca cctgctggag ggcctggtcg ccaatgcccg ctgcaccgtc cacctgctcc
     1021 tggaggccgg cgacctgccc catcatgtcc tggaggcctg cttcaaggcg ctcgcccgcg
     1081 ccctcggcga cgcctgcgcc cccgatcccc gcgccggcag cggcagcacc aaaggaaccc
     1141 tcgcatgaac ccgaaccgct ccagacctcg cgccagaccg cccgtggccc tgctcgacta
     1201 cggcgtcggc aacctgcact cggccgccaa ggcgctggac cgtgccggcg ccgaggtgcg
     1261 gacggtgcca actgtggccg ccgcggaggc ggcgggggcc tgggccatgg tggtgccggg
     1321 cgtgggcgcc taccgcgcct gcctggaggg gctcggccgg gccggcgggg ccgccgcggt
     1381 cgccgcctgg atcgacgcgg gccggccgct gctcggcatc tgcgtcggca tgcagctcct
     1441 gttcgaagcg agcgaggagg gccccgtcgg tgatggcatc ggcgcgctcg ccggcacggt
     1501 ccgccgcctc ggggggttcg gggggcatgg tcccgcggcc ggaagcactg aggccgtttc
     1561 ccagcgtggt cgcggggccc cccgatgggt cgggggcccg gtcaaggtgc cgcacatcgg
     1621 ctgggacgag gtgaccgttc agccgggggg ttcggggggc acggtcccgc ggccggaagg
     1681 agtgcgggcc gattccactg cggccgcggg gccccccgat gggtcaagga gccgcctgtt
     1741 cgccgggatc gccgacggcg cccgcttcta cttcgtccac tcctacgcac ccgaggcgag
     1801 cggcgcggcg gtcgcggcgg tctgcgacta cggcagcccc ttcgcggccg ccgtcgagcg
     1861 gggcaacctc ttcggcaccc agttccaccc ggagaagtcc agcgcggctg gtcacgcgct
     1921 gctcagcaac ttcgtcgccc tggtcgccga gtggggggcg ggggaccagg agccggaggg
     1981 agcgcggggc gccgtccggg gaatctcatg agcgcggcag cggcgaacgg gccggaccac
     2041 ggtgacgcgg cgggggaacg ggagctcacg gcgacggccg tgccgctgac gctgctgccc
     2101 gccgtcgacc tgctcggcgg gcaggcggcc aggctcgtgc agggcgccct cggcaccgag
     2161 acggcctatg gcgacccggt ggaggccgcc cgctcgttcg cggcccaggg cgccgagtgg
     2221 ctccacgtcg tggacctcga cgccgccttc ggacggggcc cgcgaaaccg tgcgcagatc
     2281 ggccggatcg tggcggcggt cgcgccggtc cgggtggagg cctccggtgg ggtgcgccac
     2341 cccgacgacc tggacgccct cctcggcctg ggtgccgccc gggtggtgat cggcaccgcc
     2401 gcgctggagg atcccgcctg ggtgggcgag gcgtgcgagc ggcacggcga ccgggtcgcc
     2461 gtcgggctcg acgcccgcgg gcgtgagctg cgggcacgcg gctggaccac cggcggcggc
     2521 gacctgttcg aggtgctgga ccgcctcgac cgagctggat gccgccgctt cgtctacacc
     2581 gaggtggccg ccgacggcat gctcagcggc ccggcgctcg acggcctgcg ggccgtgctg
     2641 gccgccacca agcggccggt ggtcgcctcc ggcggcgtcg caaccctcga tgacctgcgc
     2701 gacctcgccg cccttgcctc cggcggcctg gagggcgtga tcgtcggcaa ggctctctac
     2761 gcgggccgct tcaccgtcgc tgaggcgctc gccaccgtgc gggcggcggc cggcattggg
     2821 gaggcgcggc catgacgctc gcgcggcggg tgatcccctg cctggacgtc accgccggcc
     2881 gggtggtcaa gggtgtctcg ttcgtcaacc tggtcgacgc cggcgacccg gtggagctgg
     2941 ccgccctcta cgacgccgaa ggcgctgacg agctggtctt cctcgacatc accgccaccg
     3001 tggaggcgcg tgagaccctc ttcgacgtgg tcgcccgcac cgccgagcag gtgttcatcc
     3061 ccctcaccgt cggcgggggc gtgcgcaccc ccgacgacgt gcggcggctg ctgcgtgccg
     3121 gcgccgacaa ggtctcggtc aactccgccg ccgtccgcga cccggatctg ctctccgccg
     3181 gggcggacac ctacggggtg cagtgcatgg tgctcgccat cgacgctcgc cgccgccccg
     3241 gcgcccgccc cggcgcccgc cccagcggcg gtggcaccgg ctgggaggtg gtggtcgacg
     3301 gcggccgcac ctccaccggc ctcgacgccc tcgcctgggc cgaggagggc gccgcccgcg
     3361 gcgccgggga gctgctggtc accagcatgg acgccgacgg ccagcagacc ggctacgacc
     3421 tcgacctggt cgaggcggtc accgcggcgg tcgacatccc ggtgatcgcc agcggcggcg
     3481 ccggcaaccc tgaccatttc accgccgcct gcctggccgg cgccgacgcc gtcctcgccg
     3541 ccagcgtctt ccacttcggt cagctctcca tctcccaggt caaagacagc ctcgccgccg
     3601 ccggcctccc cgtccgcacg tagcttcgac ggtcagtcgc cggtgtcgct caggttgcgg
     3661 atctcccgca gggccaccga gaccatggcg atgtcgaccg cgtccacctc cttgaggtcg
     3721 tcgaacaggg cgaggcagcg gtccaccgca gtgcggttga cgtcgaccca ggcgttgacc
     3781 agctgctggg cgtcgcggtc ctcaactccg gggcgcagca cttgggcggt cagctcgcgc
     3841 tgctcggagt agaggtcgtc gcgcagggcc ccttgggcga gcgcctgcca gtgggtcacg
     3901 gtgggcagct tggcgatctg gtcgcgcagc cagtgcaggt cgaggcgggc gccgagcgcg
     3961 aagtaggccg cggcgaccgc ctccacggtc tcgccggcgg cgctggccac ctcgatgacg
     4021 ttcaggccgg agaacagcgc gtccatgctg gcgacccggt tggccaggtc gggtgggacg
     4081 tcgagggcga cgaagcgctc ggcggcgtgg tcgagcgact cggagtccgc gggagccagc
     4141 agcttcggca gctcctgcgc cagcgcggtg atccccggcg cgaaagacga caccgtcgcg
     4201 gcgatgtcga gcggctggcg gcggttgcgc aggaaccagc gggccgcccg ctcgatcagg
     4261 cggcggccct cggccatcag ggccgtctgg accgacgcgt cgacctggtc gtcgagctgg
     4321 ccgagctcct tccacagccg gcgggcgtcg aacacttccc gcgccaccgt gtacgcgcgg
     4381 gcgatgtccg gcgcgtgggt tccggtgtcc tcgcgcaggc ggtgcacgaa ggtggtgccg
     4441 acgcggttca ccatgctgtt ggtgatgtgg gtggcgatga tctcgcgtcg cagcgggtgg
     4501 cggcggacct ggtcgcggaa cggctcgcgg agcttggccg gcaggtagcg ctccaggtcg
     4561 tcggagaggt aggggtcctc cggcacgtcg gaggcgagca gctcctcctt cacgccgacc
     4621 ttgccgtagg cgagcagcac cgccagctcc ggccgggtga ggccgtgccc ggcggcgcgg
     4681 cgcgcgtcga gctcctcgtc gctcggcagg ccctccagcc gacggttgag ccggccggcg
     4741 cgctccaggc tgcggatcag ccggaggtgg tcgtcgaggc tggcggcggc gcgcgactcg
     4801 gtgatgctga gcgcctgcgc ctggcggtag ttgtcgtgca ccaccacctg ggcgacctcg
     4861 tcggccatct gggcgagcag gcggtcgcgc tgcttacggg tcatgtcgcc gtcggcgacc
     4921 acctggttca gcaggatctt gatgttgacc tcatggtcgg agcagtcgac cccgccggaa
     4981 ttgtcgatga agtcggtgtt gatgcggccg ccgccgagcg cgtactcgac ccggccgagc
     5041 tgggtgaagc cgagattgcc gccctcgccg accacccggc agcgcagctc ggcgccgtcg
     5101 acccggaccg cgtcgttgcc gcggtcacgg gcggcggcat gggactcgct gctcgccttc
     5161 acgtaggtgc cgattccgcc gttccacagc aggtccaccg gcgcgcgcag cagcgcgctg
     5221 atcagctcgc tgggcgtcag cgcctggacg ccggcctcca ggccgagcac ctcgcgggcc
     5281 tcggctgaca gcgggatcga cttggccgtc agcggccaga cgccaccgcc cttggagatc
     5341 gccgcggggt cgtagtcgga ccaggaggat cgggggaggg tgaacagccg ctcccgctca
     5401 gcgaagccgc gttcgggatc cgggtggggg tcgaggaaga tgtgcaggtg gttgaaggcg
     5461 ccgaccagtt tgctgtggcg cgatcgcagc atgccgttgc cgaacacgtc accggacatg
     5521 tcgccgatgc cgaccacggt gaagtcggtc gtctgcgggt tcatgttgag ggcgcggaaa
     5581 tgggccttga ccgactccca ggcaccccgg gcggtaatgc cgagcttctt gtggtcatag
     5641 ccggccgacc cgcccgaggc gaaggcgtcg tccaaccaga agccgtactc gcgggcgatc
     5701 gagttggcca ggtccgagaa cgtcgccgtg cccttgtcgg ccgcgaccac caggtagggg
     5761 tcgtcctcgt cgtagcggac cacgccggcg ggcggcatca cctggccgtc gtggtcgcgg
     5821 ttgtcggtca ggtcgagcag ccctcgcatg aacagctcgt agcaggcggc cacctcggcg
     5881 ccgagcgcct cccggtcggc gggtggcggg aggcacttga ccacgaagcc gcccttggcg
     5941 ccggtcggca cgatcaccgc gttcttgacc atctgcgcct tcatcaggcc gaggatctcg
     6001 gtgcggaagt cctcccggcg atccgaccag cggatcccgc cgcgggcgac ccggccgccg
     6061 cgcaggtgca ccgcctcggt ccgtggcgag tagacgaata cctcgaacat cggccggggc
     6121 agcggcaggc tcggcacccg ctccggatcg agcttgaacg acaggtacgg cttgggtgcc
     6181 ccgtcagcct gcacctggaa gtagttggtc cgggtcatcg cctccagcag cgccaggaaa
     6241 ctgcgcagga tgcggtcggt gtcgaggctg gcgaccgagt cgatcgcggt gccgatccgc
     6301 tcccgcagcg cctccacctc ggccgtgtcc ccgtcggagg cgcgctgcct cgccgggtcg
     6361 aagcgggcgg agaacagctc gacgaactgc cgggcgaccg ccgggttggc gcacagcgcc
     6421 tcctccatgt acgcctggct gaaggtcagc cgggtctggc gcagatactt gcagcacgcc
     6481 cgcagcagga cgatctcgcg ccagctgagc tcggcgccga gcaccagggc gttgaagccg
     6541 tcgctttcgg cctcgccgct gaggatccgg ccgaaggcgt cctggaacag ctcgcggacc
     6601 ccgccgaggt ccacctcggc catcacggcg tcaccggtcg cgcccgcccc ggccggctgc
     6661 acgcccggtg cccctggcgc gtactgcagg ccgaagtcgt gcagccacac cgaggtcatt
     6721 cccgcctccg cctccgccgc cggcgggcgg atctcgtagg ggcgttcctc gaccacctcg
     6781 acgcccatgt cctccagcat cgggaccacg tcggagagcg gcagcgggcg ctcccggctg
     6841 aacagcttga ggcgcaggga ccccagcggc gcctccagcg ggcggtacag gctcatcgcg
     6901 ttctgggcgt cgcgggcgcc agcggtgagg tcctccatcc gctggatgtc gtagacggcg
     6961 ctgcgggcgg actggtcctc gcggtaggcg gcggggaacg cctcggcgta gcgggccgac
     7021 agccggttgc cggcctcctc gccgtgctgg ccgagcagca gggcctgcag ctcgtcggtc
     7081 caggagcggg tcgtctcagc gagcttggcc tctagctcgt gctggtcgta ggggggaatc
     7141 tcgccgtcgg ggacacggat gacgaagtgc agccgggcca gcaccgactc cgacagcagc
     7201 accgcgaagt ccagcgtcga gccggggaag gcgtggcgca ggaccgcctc gatccgggtg
     7261 cgggtggcgg tggtgtagcg gtcgcggggc acgtacacca gcgccgacac gaagcgctcg
     7321 tagcgctcct tccacaggaa caggcgcacc cgccggcgct cctccaaccg caggatgccc
     7381 atggcgaccg tgaagagctg ctcgtcggcg atctggaaca gctcgtcgcg ggggaaggtc
     7441 tccaggatgt tgagcagcgc cttccagtca tgcccgccgg gcggcagacc ggccagctcc
     7501 agcacggtgg cgaccttgcg ccgcagcgcc gggatctcgc gcgggttccg gttgtaggcc
     7561 gccgaggtgt acaggccgag gaagcggtgc tcgccgatca cagcgccctg ctcgtcgaag
     7621 cgcttgaccc ccacatagtc caggtaggac ggccggtgca cggtggcgcg tgagttcgcc
     7681 ttggtcagta ccagcgggcg ccggtccctg gcctggcggc gtgcctgcgg ggggagcttg
     7741 gcgaagcttc gcgagtgcga gcgggccact gccggccccg gcgtcgccgc cttccccttc
     7801 cccttcggcc gggtgctatg gccgcgcagg atgccgagcc cgctgccggg caccgggcgc
     7861 agcacgttct cgccgtcaac gacatccagg tcgtaggcac ggtagccgag gaaggtgaag
     7921 ttgccgtcgt cgatccactc cagcagcgcc ctggtctcgg ccagctcggc cgcctcaacc
     7981 ggcgggggat gctcaccgag ttcggcgagg atcccggcga cccgctcccg catcggcgcc
     8041 cagtcctcga ccgccacccg cacgtcgccc aggacccgcc cgatccccgc ccggagctgc
     8101 gtgagcagct gcggttcggt ctggcggtcg acctcgatgt ggatgcatgc ctcccgcagg
     8161 gcgtcggtcg ggagttcggg ggactctttg gacgccccct ccggtggcag gagctgcagc
     8221 agctcgccgg ccgcgtcgcg gcggaccacc agggtcgggt ggatcggcag gtgaatggtg
     8281 aggccgtgtc ggttcagctc catcgtcacc gagtcgagca gaaacggcat gtcgtcggtg
     8341 acgatctc
//
LOCUS       DASWDC010000260         3416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_120001, whole genome shotgun sequence.
ACCESSION   DASWDC010000260 DASWDC010000000
VERSION     DASWDC010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120001"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VF995_08455"
     CDS             complement(<1..1045)
                     /locus_tag="VF995_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9343628.1"
                     /translation="MTSAPARSPLRRLLPARPLSLRARLLAVLLILVICGLGVADVAT
                     YSRLRSFLVGRVDQQLDTAHRGVELMLLGGGPPAHDRGGQIDVQYALDEASRRYQGLF
                     LQVRDSGNAVEGNVQCVGRDGTQSSPQLPAQLPTPHASGQSPSSPYQSGGPLPGGAAE
                     PAVYLNTPAVQHGAASFRVRVSPLIDGSGTLIVAQPLGDIAGTLRSLMLIEILVSVGV
                     LAGAIAFGLWLVRLGLRPLDDIAATAATIAGGDLTQRVARADAGSEVGRLGQSLNRML
                     GQIEAAFVERSASEGRARQSEARMRRFVADASHELRTPLAAIRAYAELFRRGADQHPE
                     DLPRLLRRIESEAA"
     gene            complement(1047..1754)
                     /locus_tag="VF995_08460"
     CDS             complement(1047..1754)
                     /locus_tag="VF995_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9343629.1"
                     /translation="MAAAPVSARVLVVDDEPSITDAVATALRYQGFEVQEAATGRAAL
                     AAAASFRPDFIVLDVMLPDVDGFEVARRLRSSGIGTPVLFLTAKDATEDKLSGLDIGD
                     DYVTKPFSLAEVIARVRAVLRRSHPDAQADGVLRFADLVLDEKTHEVFRSDAKIHLTA
                     TEFKLLRYFMLNPRQVLSKTQILDHVWEYDFNGDDNVVETFVSYLRRKLDRHGPPLIH
                     TIRLVGYSLRLPANEAV"
     gene            complement(1872..3197)
                     /locus_tag="VF995_08465"
     CDS             complement(1872..3197)
                     /locus_tag="VF995_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9343630.1"
                     /translation="MGAVAAVVVAAPVPGSEWSNEEDDQGRGAAEDDGGAPMIFESIR
                     LAFNRLGANAARSALTVLGVIIGVGSVVTLVAVGRGSAADVNDQFSGLGANTLTVTSG
                     RGFGRGLAGAAGSANSLKLTDLGALQKLSTVAAVAPVLQTQQTITADTTSVQSPVKGT
                     TPALMGTDHLQVASGTFFSGFAEARKLPLAVLGATLTSDLGLTPRSAIGTTLTVGPDH
                     FVVVGVLHAQGQGFSSEDDSLLVPMSSLVGRLVSGNATVNQFRVAATSAGAVNSRYRT
                     DVTNTLRATHGLDASANNDFLTIDPTTLIQARQASSTNFTRLITAIAAISLVVGGIGV
                     ANVMLVAVRERTHEIGIRRAIGARRRDVIAQFLTEATVLSVLGGLLGVAGGLGLTYAL
                     PHVTGQRTIASYPAAALALAISALVGILAGIGPANQAAGLDPADALRYE"
     gene            complement(3136..>3416)
                     /locus_tag="VF995_08470"
     CDS             complement(3136..>3416)
                     /locus_tag="VF995_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HEX9343631.1"
                     /translation="VIVPTSAIGGGANNPTVQVELGDGQTVARQVIVGLSTASGSEII
                     AGVRPGDQVVTGVSASQNTTATTQATNQRGGGGGGGGGGAGPGFGVVQ"
BASE COUNT          463 a         1179 c         1285 g          489 t
ORIGIN      
        1 gggcggcctc gctctcgatc cggcgcagga ggcgcgggag gtcctcgggg tgctggtcgg
       61 cgccacggcg gaacagctcg gcgtaggcac ggatcgcggc caggggggtg cgcagctcgt
      121 gggaggcgtc cgcgacgaac cggcgcatcc gcgcctcgga ctgccgcgcc cgcccttcgg
      181 acgcgctccg ttcgacgaac gcggcctcga tctggccgag catgcggttg agcgactggc
      241 cgagccggcc gacctcgctg ccggcgtcgg cccgcgcgac ccgctgggtg aggtccccgc
      301 cggcgatggt ggcggcggtc gcggcgatgt cgtccagcgg ccgaagcccc agccggacca
      361 gccacagccc gaaggcgatc gcgcccgcga gcaccccgac cgagaccagg atctcgatca
      421 gcatcaggct gcgcagcgtc ccggcgatgt caccgagcgg ctgcgccacg atcagggtgc
      481 cgctcccgtc gatcagcggc gagacccgaa cccggaagga cgccgcgccg tgctggacgg
      541 ctggggtgtt caggtagacg gccggctccg ccgctcctcc tggcagcggc ccgccgctct
      601 ggtatggcga ggacggcgac tggccgctcg cgtggggcgt ggggagctgt gcggggagct
      661 gcgggctgga ctgcgtcccg tcccggccga cgcactgcac gttgccttcg acggcgttgc
      721 cgctgtcacg gacctgcagg aagaggccct ggtagcggcg gctcgcctcg tcgagggcgt
      781 attgcacgtc gatctggccg ccacggtcgt gcgcgggcgg gccgccgccc aggagcatca
      841 gctccacgcc ccggtgggcc gtgtcgagct gctgatcgac ccgcccgacc aggaaggagc
      901 gcagccgcga gtaggtggcc acgtcggcga cgccgagccc gcagatcacc aggatcagca
      961 ggaccgccag cagccgcgcc cgcagcgaga gcgggcgtgc cggcagcagc cgccgcagcg
     1021 gcgagcgggc tggcgcgctg gtcatgtcac acggcttcgt tcgctgggag ccgcaggctg
     1081 tagccgacca gccggatggt gtggatcagc ggcgggccgt ggcggtccag cttgcggcgc
     1141 aggtagctga cgaaggtctc gacgacgttg tcgtctccgt tgaagtcgta ctcccagacg
     1201 tggtcgagga tctgtgtctt ggacaggacc tggcgcgggt tgagcatgaa gtagcgcaac
     1261 agcttgaact cggtggcggt caggtggatc ttggcgtcgc tgcggaagac ctcgtgggtc
     1321 ttctcgtcca ggaccaggtc ggcgaagcgc agcacgccgt cggcctgggc gtcaggatgg
     1381 ctgcggcgca ggaccgcccg gacccgggcg atcacctcgg cgaggctgaa gggcttggtc
     1441 acgtagtcgt cgccgatatc gaggccgctg agcttgtcct cggtggcgtc cttggcggtc
     1501 aggaacagca ctggggtgcc gatgccgctg ctgcgcaggc gccgggcgac ctcgaagccg
     1561 tcgacgtcgg gaagcatcac gtcgagcacg atgaagtcgg gccggaacga cgccgccgcc
     1621 gccagcgccg cccggcccgt cgctgcctcc tgcacctcga atccctggta gcgcagggcg
     1681 gtcgcgaccg catcggtgat cgacggctcg tcgtccacga ccaggacgcg cgccgacacc
     1741 ggtgccgctg ccatctcgcc tccccctcga actgcccgac ctgaactgcc cgatccttcc
     1801 agatcgagta tgccgcgcct acctgtgtgt ccgctgtggc ggcttcctcc ctcaagctgg
     1861 ggccgtctcg gttattcgta gcgaagcgcg tcggccgggt ccaggccggc cgcctgattg
     1921 gccgggccaa tcccggcgag gatcccgacc agggcggaga tcgcgagcgc gagcgccgcc
     1981 gccgggtacg aggcgatcgt gcgctggccg gtgacatggg gcaaggcgta ggtgagcccg
     2041 agaccgccgg ctacgccgag caggccgccg aggacgctga ggacggtcgc ctcggtgagg
     2101 aactgggcga tcacgtcgcg gcggcgggcg ccgatcgccc ggcggatgcc gatctcgtgg
     2161 gtgcgctccc ggacggccac cagcatcacg ttggcgacgc cgatgccgcc gaccaccagg
     2221 ctgatcgcgg cgatcgcggt gatcaggcgc gtgaagttgg tggaggacgc ctggcgggcc
     2281 tggatcaggg tggtcgggtc gatggtcagg aagtcgttgt tggcgctggc gtccaggccg
     2341 tgcgtggcgc gcagcgtgtt ggtcacgtcg gtgcggtagc ggctgttgac cgctccggcg
     2401 ctggtggcgg ccacgcggaa ctggttgacc gtggcgttcc cgctcaccag ccgcccgacc
     2461 agcgacgaca tcggcaccag cagactgtcg tcctcgctgg agaacccctg gccctgggca
     2521 tggagcaccc cgacgaccac gaaatggtcg ggcccgacgg tgagggtggt gccgatcgcc
     2581 gagcggggcg tcagcccgag gtcggaggtc agggtcgcgc ccaggaccgc caggggcagc
     2641 ttgcgggcct ccgcgaagcc gctgaagaag gtgccggagg cgacctgcag gtggtcggtg
     2701 cccatcagtg cgggcgtggt gcccttgacc ggcgactgca cgctggtggt gtcagcggtg
     2761 atggtctgct gggtctgcag caccggggcg accgccgcga ccgtgctgag cttctgcagg
     2821 gcgccaaggt cggtcagctt gaggctgttg gccgacccgg cggcaccggc gaggccgcgc
     2881 ccgaagccgc gcccgctggt gactgtcagg gtgttcgcgc cgagcccgct gaactggtcg
     2941 ttcacgtcgg cggcggagcc acgcccgacc gccaccaggg tgaccaccga cccgactccg
     3001 atgatcaccc cgagcacggt cagcgccgac cgcgccgcgt tcgcccccag gcggttgaag
     3061 gccagccgga tcgactcgaa gatcatgggc gcgccgccgt cgtcctccgc cgccccgcgt
     3121 ccctggtcgt cctcctcatt ggaccactcc gaacccggga ccggcgccgc caccaccacc
     3181 gccgccaccg cccccacgct ggttggtggc ctgggtggtc gcggtggtgt tctggctggc
     3241 ggagacgccg gtcacgacct ggtcgcctgg ccggacgccg gcgatgatct cactgccgct
     3301 ggcggtggag aggccgacga tcacctggcg ggcgaccgtc tggccgtcgc ccagctcgac
     3361 ctgcacggtc gggttgttgg cgccgccgcc gatggcggag gtcggcacga tcacca
//
LOCUS       DASWDC010000261         5013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_793868, whole genome shotgun sequence.
ACCESSION   DASWDC010000261 DASWDC010000000
VERSION     DASWDC010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793868"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VF995_08475"
     CDS             <1..367
                     /locus_tag="VF995_08475"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEX9343632.1"
                     /translation="AEVDFSKLASSAIKVSADRRTATITLPRAQLSQVHIDVDHSYVF
                     SRQRGVLDRIGSVFAEDSNSLQQLYQVAEVKLAAAAQQSGVADRAEQNTRATLEGMLH
                     SLGYTTVIVNFTGPAAPPK"
     gene            397..2067
                     /locus_tag="VF995_08480"
     CDS             397..2067
                     /locus_tag="VF995_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX9343633.1"
                     /translation="MTGAAGRVEDWFLTAEERGNPATSTSAWTEGNLAEPLIHGASYY
                     TRLLQALRTLRPGDQLAIADWRGDADQLLDGPGSELGRILCDLAERGVGVRGLLWRSH
                     PSRAHFNEEQNRHLGEIVNQAGGQILLDERVRGPGSQHQKLVLLRHDGREDLDVAFLG
                     GIDLCHGRRDDERHLGDPQSTPMDERYGPRPPWHDVQLEVRGPAIGDLSITFRERWED
                     PTPLDHHNPWRARLTRKVHQPRHPDPLPPVLRDPAPVERAGERSDAVHGRTPAEAEAE
                     PAGTHAVQILRTYPAKRHPLPFAPEGERSVARAYSKALSRAHRFVYLEDQYLWSAEIA
                     QVLVVALRRAPQLRLVAVVPRYPDQDGPFTGPPNRIGQQTALDQVKAAGGDRVAVFDL
                     ENEQGTPVYVHAKVCVIDDVWAAVGSDNLNRRSWTNDSELTAAVLDPTLDRRLPADPA
                     GLGDGARVFARELRLTLWREHLGFGPDDDPAPLLDPERGFQLWRDRAAALEAWHRAGR
                     TGPRPPGRIRDHRPGRVQPWAVWWAWPLYKTIVDPDGRPRFLRRTGKF"
     gene            complement(2078..3085)
                     /locus_tag="VF995_08485"
     CDS             complement(2078..3085)
                     /locus_tag="VF995_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9343634.1"
                     /translation="MEFQGTFDERTITSEALGDNPLGDPHERPLWVYLPPGAGDDPSV
                     RYPSIYLIQGLTGQVDMWWNRTPFRPTMPERIDQLFAGHGAPPAIVVFVDAWTSLGGS
                     QYLNSPGTGRYLDYLCDEVVAFVDSHYPTLPGREHRGLTGKSSGGYGAMVVPMLRPDV
                     FGALASHAGDALFELCYLPDIAKAVRALRDDYEGSYEKFWADFNSRPAFSKEHDGDLL
                     NAYCMAACYSAEPDGTVTLPFDVATGKLRDDIWQRWLALDPVRMAPSHADDLRSMRGI
                     YLDAGKRDEYFLDLGAAAFAAELDTIGVSYTFELFEATHAAIEYRYPRAIAFLAEHLR
                     Q"
     gene            complement(3132..4118)
                     /gene="hemB"
                     /locus_tag="VF995_08490"
     CDS             complement(3132..4118)
                     /gene="hemB"
                     /locus_tag="VF995_08490"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017585817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX9343635.1"
                     /translation="MQSPAPFPASRPRRLRRSGVLRELVRETTLSRRDLVAPLFVKEP
                     LGAPEPIASMPGHWQHTLESLRKEAAELAALGIPALVLFGIPTDKDPDGTQAWAPEGI
                     IQQALRALHNDLGDDVELIADLCLCEYTSHGHCGVLGPDNSVDNDGTLERYARIAVAQ
                     AEAGATIIAPSGMMDGQVGTIRQALDQAGFTDLPILAYAAKYASGLYGPFRDAAEGAP
                     QFGDRTGYQMDPANADEALREVALDVAEGADMVMVKPAHAYLDVIWRVKEAFGLPTAA
                     YHVSGEYALLKAAAERGWIDGERVMLEILTAIRRAGADMVITYHAREAAPLL"
     gene            4246..4623
                     /locus_tag="VF995_08495"
     CDS             4246..4623
                     /locus_tag="VF995_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343636.1"
                     /translation="MFRTTLLQLAAPDALRGRISALWLAQVSASPRIGDAEAGAVAAI
                     STPQISVISGGLACIAGALALVWLIPGLRHARTAAPASSGEAAGPQPEVLQEAAGASA
                     SKISSAAASDAPGSASGEPNSGS"
     gene            complement(4527..>5013)
                     /locus_tag="VF995_08500"
     CDS             complement(4527..>5013)
                     /locus_tag="VF995_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9343637.1"
                     /translation="GDVAEFHGQYYEVPPSRMDPKPLQRPHPPLLLGGSAEPALRRAG
                     RLADGWISASNADLAHIGEPIATVKQAAREAGRDPESLRFVSRGPLRVRPAGQPDRRP
                     LHGSYDDIRADLDRLREQGVTEVFFDLNYDPEFGSPDADPGASLAAAEEILEALAPAA
                     S"
BASE COUNT          696 a         1813 c         1824 g          680 t
ORIGIN      
        1 cgcggaggtc gacttctcca agctcgccag cagcgcgatc aaagtctccg ccgaccgccg
       61 caccgccacg atcaccctgc cccgcgcgca gctcagccag gtgcacatcg acgtggacca
      121 cagctacgtc ttcagccgcc agcgcggcgt gctggaccgg atcggctcgg tgttcgccga
      181 ggattccaac agcctgcagc agctctacca ggtcgccgag gtcaagctgg ccgccgccgc
      241 gcagcagagc ggcgtcgccg accgggccga gcagaacacc cgcgccacgc tggaggggat
      301 gctccactcg ctcggctaca ccacggtcat cgtcaacttc accggcccgg ccgccccgcc
      361 gaaatgagac tgcgccggag ccgaggtgac cgtgacgtga ccggcgctgc cggccgcgtc
      421 gaggactggt tcctgacggc ggaggagcgc ggcaacccgg cgacctcgac cagcgcctgg
      481 accgagggca acctcgccga gccgctgatc cacggcgcca gctactacac ccggctgctg
      541 caggccctgc gcaccctgcg gcccggtgac cagctcgcca tcgccgactg gcgcggcgac
      601 gccgaccaac tgctcgacgg gcccggcagc gagctgggcc ggatcctctg cgacctggcc
      661 gagcgaggcg ttggggtccg ggggctgctg tggcgctccc acccgagccg ggcacacttc
      721 aacgaggagc agaaccgcca cctcggcgag atcgtcaacc aggcgggcgg gcagatcctg
      781 ctggacgaac gggtccgcgg acccgggtcg cagcaccaga aactggtgct gctgcgccac
      841 gacggccgtg aggacctcga cgttgccttc ctcggcggga tcgacctgtg ccacggccgc
      901 cgtgacgacg agcgccacct cggcgacccg cagtccaccc cgatggacga acgctacggg
      961 ccccggccgc cctggcatga cgtgcagctg gaggtccgcg gcccggccat cggcgacctt
     1021 tcgatcacct tccgggaacg ctgggaggac cccactcccc tcgaccacca caatccctgg
     1081 cgtgctcgcc tcacccgcaa ggtccaccag ccccgccacc ccgacccgct gccgccggtc
     1141 ctgcgcgacc cagcgccggt cgagcgtgca ggcgagcgaa gcgacgccgt ccatgggagg
     1201 acgccggccg aggcggaggc ggagccggcc gggacgcacg cggtgcagat cctgcgcacc
     1261 tacccggcca agcggcaccc gctgccgttc gcaccggagg gcgaacgcag cgtcgcccgc
     1321 gcctactcca aggccctcag ccgcgcccac cggttcgtct acctggagga ccagtatctc
     1381 tggtccgcgg agatcgcgca ggtcctggtg gtcgccctgc gccgcgcgcc gcagctgcgc
     1441 ctggtcgcgg tggtgccccg ctaccccgac caggacggcc ccttcaccgg cccgcccaac
     1501 cggatcggcc agcagaccgc cctcgaccag gtcaaggcgg ccggcggcga ccgcgtcgcc
     1561 gtcttcgacc tggagaacga gcagggcacc ccggtctacg tccacgccaa ggtctgcgtg
     1621 atcgacgacg tctgggcggc ggtcggctcc gacaacctga accgccgctc ctggaccaac
     1681 gactcggagc tgaccgccgc ggtcctcgat ccgaccctcg accgccgcct cccggccgac
     1741 cccgccggcc tcggcgacgg tgcccgggtc ttcgcccgcg agctgcgcct caccctctgg
     1801 cgggaacacc tcggcttcgg cccggacgac gaccccgccc ccctgctcga ccccgaacgc
     1861 ggcttccagc tctggcgcga ccgcgccgcc gccctcgaag cctggcaccg cgccggccgc
     1921 accggccccc gcccccccgg ccgcatccgc gaccatcgcc ccggccgcgt ccagccttgg
     1981 gcggtctggt gggcctggcc cctctacaag accatcgtcg accccgacgg ccgcccccgc
     2041 ttcctccgcc gcaccggcaa attctgacgc gctgccgcta ctgccgcagg tgttcggcga
     2101 ggaaggcgat cgccctgggg tagcggtatt cgatggcagc gtgggtggct tcgaacagct
     2161 cgaaggtata ggagacgccg atggtgtcca gttcggcggc gaaggcggcg gcgcccaggt
     2221 cgaggaagta ctcgtcgcgc ttgccggcgt cgaggtagat accgcgcatc gagcggaggt
     2281 cgtcggcgtg gctgggggcc atgcggacgg ggtcgagcgc gagccagcgt tgccagatgt
     2341 cgtcgcggag cttgccggtg gcgacgtcga aggggagggt cacggtgccg tcgggttcgg
     2401 cggagtagca ggcggccatg cagtaggcgt tgagcaggtc gccgtcgtgc tctttcgaga
     2461 aggccggccg ggagttgaag tcggcccaga acttctcgta ggaaccctcg tagtcgtcgc
     2521 ggagggcgcg gaccgccttg gcgatgtcgg gcaggtagca cagctcgaac agggcgtcgc
     2581 cggcgtggct ggcgagggcg ccgaagacgt cggggcgcag catcgggacc accatggcgc
     2641 cgtagccgcc gctggacttg ccggtgaggc cgcggtgctc gcggccgggc agggtcgggt
     2701 agtggctgtc cacgaacgcg accacctcgt cgcagaggta gtcgaggtag cggccggtgc
     2761 ctggtgagtt caggtactgg ctgccgccga gcgaggtcca ggcgtcgacg aacaccacga
     2821 tcgcgggcgg cgcgccgtgg ccggcgaaca gctggtcgat gcgctcgggc atggtgggac
     2881 ggaagggggt gcggttccac cacatgtcca cctggccggt gaggccctgg atcaggtaga
     2941 tgctcgggta gcgcacgctg gggtcgtcac cggcgccggg gggcaggtag acccagaggg
     3001 gccgctcgtg cgggtcgccg agggggttgt cccccagcgc ctcgctggtg atggtgcgct
     3061 cgtcgaaggt tccctgaaat tccacgtgcc acgggcgcaa cgaaagcctc cttgcgttgt
     3121 tcggggtcgc ttcagagcag cggtgcggcc tcgcgggcat ggtaggtgat caccatgtcg
     3181 gcgcccgccc ggcggatggc ggtcaggatc tcgagcatga cccgctcgcc gtcgatccag
     3241 ccgcgctcgg cggcggcctt gagcagcgcg tactccccgg agacgtgata ggcggcggtg
     3301 ggcaggccga aggcctcctt gacccgccag atgacgtcca ggtaggcgtg cgcgggcttg
     3361 accatcacca tgtcggcgcc ctcggcgaca tccagggcga cctcccgcag ggcctcgtcg
     3421 gcgttggcgg ggtccatctg gtagccggtg cggtcgccga actgcggggc cccctcggcc
     3481 gcgtcgcgga acggcccgta cagcccggag gcgtatttgg ccgcgtaggc gaggatcggc
     3541 aggtcggtga agcccgcctg gtcgagggcc tggcggatgg tgccgacctg gccgtccatc
     3601 atgccgctcg gcgcgatgat cgtcgctccc gcctcggctt gggcgaccgc gatgcgggca
     3661 tagcgctcca gcgtcccgtc gttgtccacc gaattgtccg ggcccaggac gccgcagtgg
     3721 ccgtggctgg tgtactcgca aaggcagagg tcggcgatca gctccacgtc atcgccgagg
     3781 tcattgtgga gggcgcggag ggcctgctgg atgatccctt ccggcgccca tgcctgggtg
     3841 ccgtcggggt ccttgtcggt ggggatgccg aacagcacca gcgccgggat accgagggcg
     3901 gccagctcgg cggcttcctt gcggaggctc tccagggtgt gctgccagtg gccgggcatg
     3961 gaggcgatcg gctcgggggc gccgaggggt tccttcacga acagcggcgc gaccagatcg
     4021 cggcgcgaca gggtggtctc gcggaccagc tcgcgcagga ccccgctgcg gcgaaggcgg
     4081 cggggacggc tggcgggaaa cggcgctggt gactgcatcg ggcactcctg gcgctgcacg
     4141 gacagtgagc ctgaccaccc taccgcagca ggcccagccg cgagcctggc ccgtgccgat
     4201 gctgctatca atgaggcatg gccgccccca gacgtcgtct cggaggtgtt ccgcaccacg
     4261 ctgctgcagc tcgccgcgcc agacgccctg cggggccgga tcagcgccct gtggctcgcc
     4321 caggtgagcg cctcaccgcg gatcggcgac gcggaggccg gcgcggtcgc cgcgatctcc
     4381 accccgcaga tctcggtgat ctccggcggg ctcgcctgca tcgccggggc gctcgccctc
     4441 gtctggctga tccccgggct ccgccacgcc cgcacggccg cgccggcgtc gagcggggag
     4501 gccgccggcc ctcaacccga agttcttcag gaggccgcgg gggcgagcgc ctccaagatc
     4561 tcctcggccg ccgccagcga cgcgcccggg tccgcgtccg gggagccgaa ctccgggtcg
     4621 tagttgaggt cgaagaagac ctcggtgacg ccctgctccc gcagccggtc gaggtcggcg
     4681 cggatgtcgt cataggaacc atgaagcgga cgccggtcgg gctggccggc ggggcgcacc
     4741 cgcagcgggc cgcgggagac gaagcggagc gactcggggt cgcggcccgc ctcgcgggcg
     4801 gcctgcttga ccgtggcgat cggctcgccg atgtgggcca ggtcggcgtt gctggcgctg
     4861 atccagccgt cggcgaggcg gccggcgcgc cgcagcgctg gctcggcgct gccgccgagc
     4921 agcagcggcg ggtgggggcg ctgcagcggc ttggggtcca tccgcgacgg cgggacctcg
     4981 tagtactggc cgtggaactc ggccacgtcc ccg
//
LOCUS       DASWDC010000262         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_572967, whole genome shotgun sequence.
ACCESSION   DASWDC010000262 DASWDC010000000
VERSION     DASWDC010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572967"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VF995_08505"
     CDS             <1..883
                     /locus_tag="VF995_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9343638.1"
                     /translation="ANANPLEAVLGGGRAALPRLALGGLLVAAGMGYVLGNVVVNGKV
                     VNAVSGVLLSIAVTAAGLTLIFGPWVMRLARQVGEERRARIRSEERAEVAAHLHDSVL
                     HTLALIQRADAPLEVATLARRQERELRAWLYGVHAPVAASAGLPWTASLGSPARSAGG
                     RRERLGAAVEALASKIEELYAIKVDAVVVGDEPLDERSRAVLLACQEAAVNAAKHSGA
                     PLVSVYVEAEPEAITAYVRDQGKGFDPEATPPDRHGIADSIRGRIQRQGGTVAIASEP
                     GAGTEVTITMPRPREGR"
     gene            880..1542
                     /locus_tag="VF995_08510"
     CDS             880..1542
                     /locus_tag="VF995_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9343639.1"
                     /translation="MSVPARGAGGPIRVFLVDDHQFFLSGLRAELGAQFAIVGAAADA
                     DAAIEGVRRTQPDVVLLDVHLPGGGGRAVLEAVRQTHPEIRFLALSVSDAADDVIGVI
                     RAGARGYVTKSISPPELASAIRRVNGGDAVFSPRLAGFVLDAFHGELTTPLDPELDQL
                     TAREREVLRHIARGFTYKEVARRLHLSVKTVETHVSSVLRKLQLSSRHELTRWASDRR
                     LV"
     gene            1539..2336
                     /locus_tag="VF995_08515"
     CDS             1539..2336
                     /locus_tag="VF995_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343640.1"
                     /translation="MSAGVALGLVAATFVASFVEFVEALTIVLAMGVTRGWRSALAGT
                     AAAALALAAFTAVAGYAIATWLPESLLRLVIGGLLLVFGLQWLRKAILRSSGLKALHD
                     EEETFAAQAAAAEQAGRRMAFGLDWFSFVVSFKGVFLEGAEVVFIVLTFGQNARNIPV
                     AAAAAAAAGFVVVVAGIVVQGPLARVPENTLKYVVGLMLTTFGTFWSVEGLGIARPGG
                     ESLTWPGGDLMLPVVLLAWLALSRLLIRTAPTLATPTPVTASQAKES"
     gene            2342..2566
                     /locus_tag="VF995_08520"
     CDS             2342..2566
                     /locus_tag="VF995_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343641.1"
                     /translation="MIAVRFVRAFLRFWYDFVIGDDWKIATSVAVVLVTGALLAHNTH
                     LPDGATAVLLGLALLAAFVVEFAIDTRHRR"
     gene            complement(2623..3129)
                     /locus_tag="VF995_08525"
     CDS             complement(2623..3129)
                     /locus_tag="VF995_08525"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9343642.1"
                     /translation="MPGAEGHADGCRIGSAPGIALQPGIGAQVGEPVRRDAADRGGFW
                     LVEQYRYTVGGRFWEFPQGTFPHGQDGDPAELARSELAEETGLRAGHLEHLARLHIAY
                     GMSSQGCNVFLATSLEHGPARRECEEQDMCQQWVARSSFEQMIGDGAITDTATIATYA
                     LLRVREAN"
BASE COUNT          405 a         1202 c         1108 g          495 t
ORIGIN      
        1 cgccaacgcc aacccgctgg aggcggtgct cggtggcggc cgggccgcgc tgccgcgtct
       61 ggccctcggc ggcctgctgg tcgccgccgg gatgggctac gtgctcggca acgtggtggt
      121 gaacggcaag gtcgtgaacg ccgtgagcgg cgtgctgctg tcgatcgcgg tgaccgccgc
      181 cgggctcacc ctgatcttcg gcccctgggt gatgcggctc gcccgacagg ttggcgagga
      241 gcggcgcgcc cgcatccgct ccgaggagcg cgccgaggtc gccgcccacc tgcacgactc
      301 ggtgctgcac accctggcgc tgatccagcg tgccgacgcg ccgctggagg tcgccaccct
      361 ggcccgccgc caggagcggg agctgcgcgc ctggctctac ggagtgcacg cccccgtcgc
      421 cgcctccgcc ggcctcccgt ggacagcatc gcttggctcg cctgcacgct cggccggcgg
      481 ccgccgcgag cggctcggcg cggcggtcga ggcgctcgcc tccaagatcg aggagctgta
      541 cgcgatcaag gtcgacgcgg tggtggtcgg cgacgaaccg ctcgacgagc gctcccgcgc
      601 ggtgctgctc gcctgccagg aagcggccgt caacgccgcc aagcactccg gcgccccgct
      661 ggtcagcgtc tatgtggagg ccgagcccga ggcgatcacc gcctacgtcc gcgaccaggg
      721 caagggtttc gacccggagg caaccccgcc ggaccgccac ggcatcgccg actcgatccg
      781 cggccgcatc cagcgccagg gcggcaccgt ggccatcgcc agcgagcccg gcgccggcac
      841 cgaggtgacc atcaccatgc cacggccaag ggagggacga tgagcgttcc cgcgcggggc
      901 gccggcggcc ccatccgggt gttcctggtc gacgaccacc agttcttcct ctccgggctg
      961 cgggccgagc tgggggccca gttcgccatc gtcggcgccg ccgccgacgc ggacgcggcg
     1021 atcgagggcg tccgccgcac ccagcccgac gtggtcctgc tggacgtgca cctgcctgga
     1081 ggcgggggcc gggcggtgct ggaggcggtc cgccagaccc acccggagat ccgcttcctg
     1141 gccctgtcgg tctccgacgc cgccgacgac gtcatcgggg tgatccgcgc tggcgcacgc
     1201 ggctacgtca ccaagtcgat ctccccaccc gagctggcct cggcgatccg ccgcgtgaac
     1261 ggcggcgacg cggtcttctc gccgcgcctg gccgggttcg tgctggacgc cttccacggc
     1321 gagctgacca cccccctcga cccggagctg gaccagctca ccgcccgcga acgcgaggtc
     1381 ctgcgccaca tcgcccgagg cttcacctac aaggaggtcg cccggcgcct ccacctgtcg
     1441 gtcaagaccg tcgagaccca cgtctcgtcg gtgctgcgaa agctgcagct ctccagccgc
     1501 cacgagctga cccgctgggc ctcggaccgg cgcctggtat gagtgctggc gtcgccctgg
     1561 ggctggtcgc cgccaccttc gtggcgtcgt tcgtggagtt cgtcgaggcg ctgacgatcg
     1621 tcctggcgat gggggtgacg cgcggctggc gctccgcgct cgccgggacc gccgccgccg
     1681 cgctggcgct ggcggcgttc accgcggtcg ccggctacgc gatcgccacc tggctgccgg
     1741 agtcgctgct gcggctggtg atcggcggcc tgctgctggt cttcggcctg cagtggctcc
     1801 gcaaggcgat cctgcggtcc agcggcctca aggccctcca cgacgaggag gagaccttcg
     1861 ccgcgcaggc cgccgccgcc gagcaggccg gccgccggat ggccttcggc ctcgactggt
     1921 tctccttcgt ggtctccttc aagggcgtgt tcctggaggg cgccgaggtc gtcttcatcg
     1981 tgctcacctt cggccagaac gcccgcaaca tcccggtcgc ggccgccgcc gcagccgccg
     2041 ccgggttcgt cgtggtggtc gccgggatcg tcgtccaagg gccgctggcg cgggtccccg
     2101 agaacaccct caagtacgtg gtcggcctga tgctgaccac cttcggcacc ttctggtcgg
     2161 tggagggcct cggcatcgcc cgccccggcg gcgaaagcct cacctggccc ggcggcgacc
     2221 tgatgctccc agtcgtcctg ctcgcctggc tcgccctgag ccgtctcctg atccgcaccg
     2281 cccccaccct ggccaccccg acccccgtca ccgcttcgca ggcgaaggag tcctgagcgc
     2341 catgatcgcc gtcaggttcg tccgggcctt cctgcggttc tggtacgact tcgtcatcgg
     2401 cgatgactgg aagatcgcca cctccgtcgc cgtggtgctc gtgaccggtg cgctcctcgc
     2461 ccacaacacc cacctgcccg acggagcgac agcggtcctg ctcgggctcg cgctgctggc
     2521 agccttcgtg gtcgagttcg cgatcgacac ccgccaccgg cgttaggctg atccgaacct
     2581 cgcgagccga cttcgaactt cgaggagcat gggcctcatg ctctaattcg cctcgcgcac
     2641 gcgcagcaag gcgtatgtgg cgatggtcgc agtatcggtg atggcgccat caccgatcat
     2701 ctgctcgaag ctgctgcggg caacccactg ctggcacatg tcctgctctt cacattcgcg
     2761 ccgcgccggg ccgtgttcca gtgaggtcgc caggaacaca ttgcagccct gactgctcat
     2821 tccgtaggcg atgtgaagcc tcgccagatg ctcaagatgc ccggcacgaa ggcccgtctc
     2881 ctcggcgagc tcgctacggg caagctcagc cgggtcgcca tcctgcccgt gggggaaggt
     2941 tccctgtggg aactcccaga accggccacc gacggtgtag cggtactgct cgaccagcca
     3001 aaagccgcca cggtcggcgg catcgcggcg gacgggttct ccgacctgcg cgccgatgcc
     3061 gggttgcagc gcgatccccg gtgcggaccc gatgcgacac ccatcggcgt ggccctccgc
     3121 accggggatg ctgtgcgtcg ctcacttgca ggggggcagg gtcgtggtgg tcgccgaggt
     3181 ggtggtggtc gacccggacg tgggcggcag
//
LOCUS       DASWDC010000263         4229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1241623, whole genome shotgun sequence.
ACCESSION   DASWDC010000263 DASWDC010000000
VERSION     DASWDC010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241623"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="VF995_08530"
     CDS             <1..1550
                     /locus_tag="VF995_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9343643.1"
                     /translation="STGLDLPVGDAALAVGTGVLITLVAAFAPARKATLIAPIAALRT
                     TTPAVGGFSTRRPSMQASEAMPSMGGRRSRLVSGAVVAVAGSAALLAGLFGHTGAGSL
                     LVGGGALAAFIGVTVLCPLFAKPLAGAIGAPLRRLPGRTGVLARDNAMRNPKRTAATA
                     AALMVGLALVAASAVLDASLKRAADQAIDQGTLAELYVQPADPDSGLDPALAHSIAAQ
                     PGVAAVGEVRETEGTIAGASFQKVDGVDPAVVGQVADLKVKSGSVAALSDAAGNLLVS
                     ASAAKSHHWTVGSAVPVKLDKSKGAGTLRVVGTFAGKGSFGDYLISLTSYDQITGQPT
                     DTLLMVKAVPGAPVAALKARISGLLGAYPGAKVLDQAGFKQATGATLDQILNLLVALL
                     ALAVVIALLGIVNTLALSVVERTRELGLLRAIGMRRGQVGAMIAGEAMIIAIFGALLG
                     IVLGVGLGSALAAALTRGNGAAVVAIPGTQLLVYVIGAGFAGILAAIAPARRAAKLNV
                     LTAIASE"
     gene            1767..3383
                     /locus_tag="VF995_08535"
     CDS             1767..3383
                     /locus_tag="VF995_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343644.1"
                     /translation="MRRARCILFTIAILGALLAVVVPSGAPAGAANATPLWMLHTQRF
                     SGGISGGVRAMASPEATAAQARHAGVVAATGSGLHNVQMNDDSNPPLPQNETSAAASL
                     ANPLVAVAASNDYVSGGVAIMRTANGGRSWATTRVVPQFGGTRDFCTGGDPAVAYSLR
                     DHAFYVAQLCFFRALPFSEVQVYKSIDNGKTWTPGREAARAASNFDYTTGTVDASIFN
                     DKEYIAVDNTPTSPHYGRLYVTYTKFHLLADGSSDFCPIQLSYTDTIPTTNPSLTVFR
                     HTAVVPDAPGSGGVGESANQFSDPQVEKSGALDVAYGLEECNTAIDHGFRFQKSTNGG
                     ASFLASAVHVTKPGQFQDNPDPGDLLPPTAFRAPNTESLSYNPKTGGLVYVYQNDINE
                     AASGTDIDVQTSSDGGLHWSNAKVLSTAGGAPAPNDQFFPWAATDPSGATYVIWFDRR
                     RDPANRNIDTWQAVSTNSGASWTSARISTVSWNPDLGFFTSGAFIGDYNGLAVSSKAV
                     YPVWTDGRNDAIATTGIGETDIFTNVELHG"
     gene            3536..4009
                     /locus_tag="VF995_08540"
     CDS             3536..4009
                     /locus_tag="VF995_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343645.1"
                     /translation="MNQELLALFEQDQADRDGGLRPDARARDDRRRHQVEALIAAGGL
                     RDPEDYYHAAMVFQHGTKPAHYLRAHELASRGAELGVREARWLAAAAYDRWLMMNGRP
                     QKYGTQYRADGGRWMLWEVDPATTDAERAEWDVPTLAEAHARAEEMTRADPPPRR"
     gene            complement(4043..>4229)
                     /locus_tag="VF995_08545"
     CDS             complement(4043..>4229)
                     /locus_tag="VF995_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343646.1"
                     /translation="PRAKSHLLLTHARQRTRHGTVAEAAPSPYLADIEDALLEHYRTA
                     AKRPQPAAPAGDQLQLL"
BASE COUNT          626 a         1671 c         1365 g          567 t
ORIGIN      
        1 ccagcaccgg cctcgacctg ccggtcggcg acgccgcgct cgcggtcggc accggcgtcc
       61 tcatcaccct ggtcgccgcc ttcgcgccgg cccgcaaggc caccctgatc gccccgatcg
      121 ccgcgctgcg caccaccacc ccggccgtgg gggggttttc cacccgccgg ccgagcatgc
      181 aagcgagcga agcgatgccg tccatgggag gccggcggag ccgccttgtc agcggcgccg
      241 tggtggcggt ggccgggagc gccgcgctgc tcgccggcct gttcggccac accggcgccg
      301 ggagcctgct ggtcggcggc ggcgcgctcg ccgccttcat cggcgtgacc gtgctctgcc
      361 ccttgttcgc caagccgctc gccggggcga tcggcgcgcc gttgcggcgc ctgcccggcc
      421 gcaccggggt gctcgcccgc gacaacgcga tgcgcaaccc caagcgcacc gccgccaccg
      481 ccgcggcgct gatggtcggc ctcgccctgg tggccgcctc cgcggtgctc gacgcctccc
      541 tcaagcgcgc cgccgatcag gcgatcgacc agggcaccct ggccgagctc tacgtccagc
      601 ccgccgaccc cgacagcggc ctcgacccgg cgctggccca ctcgatcgcc gcgcagcccg
      661 gtgtcgccgc cgtcggcgag gtccgcgaga ccgagggcac gatcgcgggg gcctccttcc
      721 agaaggtcga cggcgtcgac cccgccgtcg tcgggcaggt cgccgacctc aaggtcaaga
      781 gcggcagcgt ggccgccctg agcgacgccg ccggcaacct gctggtctcg gccagcgccg
      841 ccaagtccca ccactggacg gtcggcagcg ccgtgccggt caagctcgac aagagcaagg
      901 gcgcgggcac cctgcgggtc gtcggcacct tcgccggcaa gggctccttc ggcgactacc
      961 tgatcagcct taccagctac gaccagatca ccggccagcc caccgacacc ctgctgatgg
     1021 tcaaggccgt ccccggagcc ccggtcgccg ccctgaaggc ccggatcagc ggcctgctcg
     1081 gcgcctaccc cggcgccaag gtcctcgacc aggccggctt caagcaggcc accggcgcca
     1141 ccctcgacca gatcctcaac ctgctggtcg ccctgctcgc gctcgccgtg gtcatcgccc
     1201 tgctcggcat cgtcaacacc ctcgccctgt cggtcgtcga gcggacccgc gagctcggcc
     1261 tgctgcgcgc catcggcatg cgccgtggcc aggtcggcgc catgatcgcc ggcgaagcca
     1321 tgatcatcgc gatcttcggc gccctgctcg gcatcgtcct cggcgtcggc ctcggcagcg
     1381 ccctcgccgc cgccctcacc cgcggcaacg gcgccgcggt cgtcgccatc cccggcaccc
     1441 agctgctggt ctacgtcatc ggcgccggct tcgccgggat cctcgccgcg atcgcccccg
     1501 cccgccgcgc cgccaagctc aatgtgctca ccgccatcgc cagcgaatag acgaagcatc
     1561 agcagccgga gggggtcgcc gccgttggcg accccctccg gttactggcg gctacggcgt
     1621 ttctcccgca aaacgtcctg gtcactgctt gctggatcta cgggagcggg acgaacaacg
     1681 ccctacggca catagccgat tgacagccaa tatgggtgag agtgtcacct gatggtggtg
     1741 ggttccctcc tcgaaaggag caacgcgtga gaagagcgcg atgcatcctg ttcacaatcg
     1801 caatcctggg cgcgctcctg gcggtggtgg tgccctccgg ggcgccggca ggcgcggcga
     1861 acgcgacacc cctgtggatg ctgcacaccc agcgcttctc cggcggcatc tccggcggcg
     1921 tccgcgccat ggcctccccg gaggcgacgg ccgcccaggc caggcacgcc ggcgtcgtcg
     1981 cggcgaccgg gtcgggcctg cacaacgtcc agatgaacga cgactccaac ccacccctcc
     2041 cccagaacga gacctcggcc gccgccagcc tcgccaaccc gctggtcgcg gtcgcggcgt
     2101 ccaatgacta cgtcagcggc ggcgtcgcga tcatgcgcac cgccaacggc ggcaggtcct
     2161 gggcgaccac ccgggtcgtc ccgcagttcg gcggcacgcg cgacttctgc accggcggcg
     2221 accccgcggt cgcctacagc ctccgcgacc acgccttcta cgtcgcgcag ctctgcttct
     2281 tccgcgccct gccgttctcc gaggtgcagg tctacaagtc aatcgacaac ggcaagacct
     2341 ggacccccgg gcgtgaggcc gcgcgggcgg cgagcaactt cgactacacc accggcacgg
     2401 tcgacgcctc gatcttcaac gacaaggaat acatcgccgt cgacaacacc ccgaccagcc
     2461 cccactacgg ccgcctctac gtcacctaca ccaagttcca cctgctggcg gacgggtcca
     2521 gcgacttctg cccgatccag ctctcctaca ccgacacgat cccgaccacc aacccgtcgc
     2581 tgacggtgtt ccggcacacc gccgtcgtcc ccgacgcgcc cggctccggc ggggtcgggg
     2641 agtccgccaa ccagttcagc gacccgcagg tggagaagag cggcgccctc gacgtcgcct
     2701 acggcctcga ggagtgcaac accgcgatcg accacggctt ccgcttccag aagtcgacca
     2761 acggcggcgc cagcttcctg gccagcgccg tccacgtcac caagcccggc cagttccagg
     2821 acaaccccga cccgggcgac ctgctgccgc cgaccgcgtt ccgcgcgccg aacaccgagt
     2881 cgctgtccta caaccccaag accggcggct tggtctacgt ctaccagaac gacatcaacg
     2941 aagcggcctc cggcaccgac atcgacgtgc agacctccag cgacggcggc ctccactgga
     3001 gcaacgccaa ggtgctcagc accgctggcg gcgccccggc cccgaacgac cagttcttcc
     3061 cctgggcggc caccgacccg tccggggcca cctacgtgat ctggttcgac cggcgccgcg
     3121 accccgccaa ccgcaacatc gacacctggc aggcggtctc caccaacagc ggcgcctcct
     3181 ggaccagcgc ccggatcagc accgtctcgt ggaaccccga cctcgggttc ttcacctcgg
     3241 gcgcgttcat cggcgactac aacggcctcg cggtctccag caaggccgtc tacccggtct
     3301 ggaccgacgg ccgcaacgac gccatcgcca ccaccggtat cggcgaaacc gacatcttca
     3361 ccaacgtgga gctccacggc tagcggccga ggcttcgtcg ggggagccca cgctgagcgg
     3421 gctcccccga gctttctctt gccgaagcga acttgtcgga aaaaaggtgt atcttcttcc
     3481 ccgcgacata ggtcgcgtgc agccgccgct tcggaccggt ttcggggagg tggggatgaa
     3541 ccaggagctg ctggcgctct tcgaacagga ccaggcggac cgcgacggcg gcctccggcc
     3601 tgatgcccgc gcccgcgacg accggcgccg gcaccaggtc gaggcgctga tcgccgccgg
     3661 ggggctccgc gaccccgagg actactacca cgcggcgatg gtgttccagc atggcaccaa
     3721 gccagcccac tacctgcgcg cgcacgagct ggccagccgt ggcgccgagc tgggtgtccg
     3781 cgaggcgcgc tggctggcgg cagcggccta cgaccgctgg ttgatgatga acggccgccc
     3841 gcagaagtac ggcacccagt accgcgccga cggcggccgc tggatgctgt gggaggtcga
     3901 cccggccacc accgacgccg agcgtgccga atgggacgtc cccacgctcg ccgaggcgca
     3961 cgcccgggcc gaggagatga cccgggcgga tccgccgccg cgccgctgat cccgcacggc
     4021 ccgtccgctc gcgagatccg ttctagagga gctgtagctg gtctccggct ggcgcggccg
     4081 gctgcggccg cttggccgcg gtccggtagt gctccagcag ggcatcctcg atgtcggcca
     4141 gataggggga gggggcggcc tccgcgacgg tgccgtggcg ggtccgctgc cgcgcgtggg
     4201 tgagcagcag gtgggacttc gcccggggc
//
LOCUS       DASWDC010000264         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_205623, whole genome shotgun sequence.
ACCESSION   DASWDC010000264 DASWDC010000000
VERSION     DASWDC010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205623"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1019)
                     /locus_tag="VF995_08550"
     CDS             complement(129..1019)
                     /locus_tag="VF995_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9343647.1"
                     /translation="MPMSEGALPSGVTFGSIEELEAALGQHAYLADRGLATAIYLSLA
                     LGKPLLLEGEAGVGKTEVAKVLAELLGRTLVRLQCYEGIDASQALYEWDYSRQLIAVR
                     AAQLGDRQADLFGTEFLVERPLLAAVRAGDQAVMLIDELDRADDEFEAFLLEILAEFA
                     VTIPEIGRVAAQRPPAVVITSNRTRELHDALKRRCLYHWIDHPSVEREVEIVRVRAPQ
                     VPETLARQVALAVSRLRRLDVAKRPGVAETIDWSQALAFLGANGLDAATATATLGTLV
                     KDHDDLELVRSQLDQVLGKE"
     gene            complement(1060..1887)
                     /locus_tag="VF995_08555"
     CDS             complement(1060..1887)
                     /locus_tag="VF995_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX9343648.1"
                     /translation="MIPASFDYEVATSADHAIALLGEHGEDAKLLAGGHSLLPLMKLR
                     LAAPTMLIDIGRLPDQSYVRDGGDHIAIGALTTHEDVHKSDLLRSQCPLLSHTAGEIG
                     DPQVRHRGTIGGSVAHADPASDVPTVLLALDAELVVQGPEGTRTLPAAGFWTGLFESA
                     IGPRDLLTEIRVPKVPDAGWSYQKFHPRAQDWAIVGVAALARDGSAAVSLTNMGEVPL
                     RASGVEQALAGGADPAAAAEQALEGTSPPNDALASADYRRHLAKVLTRRAIEEARSR"
     gene            complement(1884..>3480)
                     /locus_tag="VF995_08560"
     CDS             complement(1884..>3480)
                     /locus_tag="VF995_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018844168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9343649.1"
                     /translation="EEFIALAVARRLGTPVKWIEERSEGYLATIHGRDVIQDMEVAAT
                     DEGKLLGVRVGLLADMGAYHQLLSPIIPILGAFLYHGSYSCDAYAFSCTAVFTNKTPT
                     DAYRGAGRPEACYAIERIMDALARRVGRDPADVRALNFIPPFSEPTAVASGVFFDSGN
                     YGAALDRAKALVGYDELRKQQQEGPRNGKLLGIGLSSYIEACGVAPSQIIAALKGGAG
                     GWEAATIRCHPTGKVTVVTGTSPHGQGHETSWSQIAADALGVTPDDVEVLHGDTAISP
                     LGLDTYGSRSLAVGGTALFKAAEKVRAKAKTVAAHELEVAEEDLEWVNGRFQVAGAPD
                     KTKTIPEVAFSLWTAHNLPAGVEPGLEETAVWDPPNFTFPSGTHVCVVEVDPETGTVE
                     IVKYVAVDDCGNVINPMIVDGQIMGGIAQGIAEAMYEEAQYDENGVLMTGNMATYRIP
                     SAAELPSYTLDRVETPSTTNPLGVKGVGEAGTIAAPPAVVNAVADAVRQVGVEFVDLP
                     VSAEHVWRAISNAKANAREGGQG"
BASE COUNT          451 a         1220 c         1288 g          521 t
ORIGIN      
        1 gatgcgcccg gtgccgaccg gcagcccccg gcgccgcagc agccgcccga acccgaccag
       61 ccgccgcagc cccgcctccg ccccggcctg aacctgtgcc tgcgtcccgg cctcggcctc
      121 cattgcggtc actccttgcc gagcacctgg tcgagctggg agcggaccag ctccaggtcg
      181 tcatggtcct tgaccagcgt gccgagggtg gcggtggcgg tggcggcgtc gaggccgttg
      241 gcgccgagga aggcgagcgc ctgcgaccag tcgatcgtct cggcgacccc gggccgcttg
      301 gccacgtcga ggcggcgcag gcgcgacacg gccagggcga cctgccgggc gagcgtctcg
      361 ggcacctgcg gggcgcggac ccgcacgatc tccacctccc gttccaccga ggggtggtcg
      421 atccagtggt agaggcagcg ccgcttgagc gcgtcgtgca gctcgcgggt gcggttggag
      481 gtgatcacga ccgcgggcgg ccgttgcgcc gcgacgcggc cgatctcagg gatggtgacc
      541 gcgaactcgg cgaggatctc cagcaggaac gcctcgaact cgtcgtcggc gcggtcgagc
      601 tcgtcgatca gcatgacggc ctggtcgccg gcccggacgg cggcgagcag cggccgctcg
      661 accaggaact cagtgccgaa caggtcagcc tggcggtcgc cgagctgcgc ggcccgcacc
      721 gcgatcagct gccgggagta gtcccattcg tagagcgcct ggctggcgtc gatgccctcg
      781 tagcactgca gccgtaccag ggtccggccg agcagctccg ccaggacctt ggcgacctcg
      841 gtcttgccca cgccggcctc gccctccagc agcagcggct tgccgagggc gagcgacagg
      901 tagatcgcgg tggcaaggcc gcgatcggcc agataggcat gctggccgag cgcggcctcc
      961 agctcctcaa tggatccgaa cgtgaccccg gacgggagcg cgccctcact catcggcatc
     1021 gagcgcgctc cgcgccccgg atcgtcttcg gcttcagcgt cagcgcgatc gtgcctcctc
     1081 gatagcgcgg cgggtcagga ccttggccag gtgccggcgg tagtcggcgc tggcgagggc
     1141 gtcgttgggc gggctggtgc cctccagcgc ctgctcggcg gctgcggccg ggtcggcgcc
     1201 accagcgagg gcctgctcga ccccgctggc gcgcagcgga acctcgccca tgttggtgag
     1261 cgacacggcg gcgctgccgt cgcgggccag cgcggccact cccacgatag cccagtcctg
     1321 cgcgcgcggg tggaacttct ggtatgacca gccagcgtcc gggaccttcg gcacccggat
     1381 ctcggtcagc aggtcccgcg gcccgatcgc cgactcgaac aggcccgtcc agaacccggc
     1441 cgccggcagc gtccgcgtcc cctcaggccc ctgcaccacc agctccgcgt ccagcgccag
     1501 cagcaccgtg gggacgtcgg aggccgggtc ggcgtgagcc accgagccgc cgatggtgcc
     1561 gcggtggcgc acctgcgggt cgccgatctc cccagccgtg tgggacagca gggggcactg
     1621 cgagcgcagc aggtcgctct tgtggacgtc ctcgtgggtg gtgagggcgc cgatggcgat
     1681 gtggtcgccg ccgtcgcgca cgtagctctg gtccggcagg cggccgatgt cgatcagcat
     1741 ggtcggcgcg gccaggcgca gcttcatcag cggcagcagc gagtgcccgc cagcgagcag
     1801 cttggcgtcc tcgccgtgct cgcccagcag cgcgatggca tggtcggccg acgtggccac
     1861 ctcgtagtcg aacgaagccg ggatcacccc tggcctcctt ccctggcgtt cgccttggcg
     1921 ttcgaaatcg ctctccagac gtgctcggcg ctgaccggca ggtcgacgaa ctcgacgccg
     1981 acctggcgta ccgcgtcggc gaccgcgttg accaccgcgg gcggcgcggc gatcgtgccg
     2041 gcctcgccga cgcccttgac gccgagcggg ttggtggtcg agggggtctc gacccggtcc
     2101 agtgtgtagg agggcagctc ggcggcggac gggatcctgt aggtggccat gttgccggtc
     2161 atcaggacgc cgttctcgtc gtactgggcc tcctcgtaca tcgcctcggc gatgccctgg
     2221 gcgatgccgc ccatgatctg gccgtcgacg atcatcgggt tgatcacgtt gccgcagtcg
     2281 tcgaccgcca cgtacttgac gatctcgacc gtgccggtct cggggtcgac ctcgaccacg
     2341 cagacgtggg tgccggaggg gaaggtgaag ttcggcgggt cccacacggc cgtctcctcc
     2401 aggcccggct cgaccccggc ggggaggttg tgcgcggtcc acagcgagaa ggcgacctcg
     2461 gggatggtct tggtcttgtc tggggcacca gcgacctgga agcggccgtt gacccactcc
     2521 aggtcctcct cggcgacctc cagctcgtgg gcggcgaccg tcttggcctt ggcgcggacc
     2581 ttctcggccg ctttgaacag ggcggtgccg ccgaccgcga ggctgcggct gccgtaggtg
     2641 tccaggccga gcgggctgat ggcggtgtcg ccgtgcagca cctcgacgtc gtcgggggtg
     2701 accccgagcg cgtcggcggc gatctgcgac caggaggtct cgtgcccctg gccgtgcggc
     2761 gaggtgccgg tcaccacggt caccttgccg gtcgggtggc agcggatggt ggccgcctcc
     2821 cagccgccgg ccccgccctt cagggccgcg atgatctggg acggcgccac cccgcaggcc
     2881 tcgatgtagg acgacaggcc gatgccgagc agcttgccgt tgcgggggcc ttcctgctgc
     2941 tgcttgcgca gctcgtcgta gccgaccagc gccttggccc ggtccagtgc ggcgccgtag
     3001 ttgccggagt cgaagaagac gccgctggcg acggcggtcg gctcgctgaa cggcgggatg
     3061 aagttgagcg cgcggacgtc ggcggggtcg cggccgaccc gccgcgccag cgcgtccatg
     3121 atccgctcga tggcgtagca ggcctcggga cggccggcgc cccggtaggc gtcggtgggg
     3181 gtcttgttgg tgaacaccgc ggtgcagctg aaggcgtagg cgtcgcagct gtaggacccg
     3241 tggtacagga acgccccgag gatcgggatg atcggcgaca gcagctggtg gtaggcgccc
     3301 atgtcggcga gcaggccgac gcgcacgccg agcagcttgc cctcatcggt ggcggcgacc
     3361 tccatgtcct ggatgacgtc gcggccgtgg atggtggcga ggtagccctc ggagcgttcc
     3421 tcgatccact tgaccggggt gccgagcctg cgggcgaccg ccagggcgat gaactcctcg
//
LOCUS       DASWDC010000265        10815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_575281, whole genome shotgun sequence.
ACCESSION   DASWDC010000265 DASWDC010000000
VERSION     DASWDC010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575281"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VF995_08565"
     CDS             complement(<1..195)
                     /locus_tag="VF995_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004873134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343650.1"
                     /translation="MGDRHEVVVGAPFPAVPAVPAVPAVPITPVVPALDDPSLYINRE
                     LSWLEFNRRVLEEAQDKQVPL"
     gene            327..1277
                     /locus_tag="VF995_08570"
     CDS             327..1277
                     /locus_tag="VF995_08570"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX9343651.1"
                     /translation="MTSTERHDRRPGELLPYGTPYLIGDPAAARHGLTELRRALAHAG
                     VVAEAAVARPASASAARRLAERALNGGARLLVAVGADPIAHGLVEALHAADAAHEPDR
                     ALGLVGLGRQDLAATFGLPDDPERAASHLLGVGVFLADLGRARWQGPDSVQRSGVFAN
                     SAELGYPAEVSSRLQRATRAGRAGRLAASVTALARSHGTPVRLTLDHTTVEFRLAGLV
                     VANGQFTMGRMKVAPRALPDDGRMNVLAFEGQPLTIYARSRELYYGTHLPHPQIREYQ
                     SKLVEVATAEPLTLALDGCRVPGGPPLAVDVLPKALRIKI"
     gene            1373..2545
                     /locus_tag="VF995_08575"
     CDS             1373..2545
                     /locus_tag="VF995_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX9343652.1"
                     /translation="MTPTPTSTGYADRLARARQELVARGIDALLLGPSADLVYLTGYS
                     APLLERMTLLVVPANGEPRLVVPALEAPLAEEQLDDVPLQVAAWQETENPVALVRAAL
                     DASAALDVTGAAGGRLAVGDRLWSTFLLRLQVALPTASFTVASTITRSLRMIKDTEEL
                     DALSRAAEAIDEVVEQLTDLHWIGRSEQDLARELDQAIRAAGHEETCFVIVGSGPNSA
                     SPHHEPGDRVIQAGDVVVVDIGGRLDGYCSDTTRTLVIGEPPEGFLELYEVLRYAQLA
                     GCQAVRPGVPASAVDKACRDVIEQAGYGSYFIHRTGHGIGLEEHEDPYLVTGNDEPIE
                     PGMAFSIEPGIYLPGKFGARIEDIVICPDPDLEPDPDAPGAFRLNRTSRKLRVVDA"
     gene            2601..3728
                     /locus_tag="VF995_08580"
     CDS             2601..3728
                     /locus_tag="VF995_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343653.1"
                     /translation="MGGRRRRRRQAWCPRCDELRPGRAGGQCLACRARLVALPGVAAP
                     APDPARAAMAGRLRALLPGLRAVALTLGAAALVVGSFVAGRTTRSSAAAQGASGVTAA
                     SPSAAATTATTQPGPGVRPFGWHASHGQVTVTLRSIENGSDFSTVVLDVAGLAGGVTA
                     FNGLRIEDARGNDLTGEGTATKEPVGRDRSGFQLGGGTDYLVQFNHPIDFGAVARVEL
                     HSLTIVQPTEELVSGSLFDPALRRSIDQNGQATATATQPCPTCRLLLRCTTCKVTRIL
                     GSAYRHGRVVLLFTPADPAHSLITDDQSSEVDAPNGQVSIDTQPLASGGTMITFQASS
                     LALISPAGRPTMAFTTHVFASRQLVAQGPWRIAQTSGGAGG"
     gene            3732..4931
                     /locus_tag="VF995_08585"
     CDS             3732..4931
                     /locus_tag="VF995_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343654.1"
                     /translation="MAAWCPRCDELRPGDGRGASLSRCPECGAALADLARPRPGGRAA
                     AADPAADAAFPVSLQPPRSRLRAALLVTALALSGLGYVAGHAGGPGSGPGSGARAGAG
                     AASSTTASTSTSTLPGPLQGPGRRDQQLIPQPERRVLGWHASDGRATLTLESIARSGA
                     QNDTNSDATAVLTVRVDGLPAGQHLLGLQGLELLDAGGGVYATPVVASIGRQRGVTAN
                     LDESGSGPGRYLVSLGPAPSTATLATVRVRALIVSRPPDVAVPLGSVPPGAGPSAALQ
                     ALPEGLHDNLQVPLEVAPDLGRNAATVELSAAFVSAHRAVLVATVDLNGTGNFSGQEA
                     VVAFTSGLVADGREVCARTDVFSASQSEIPSITLDCPASLTAAARLSATFGSATSTVA
                     FQRTITR"
     gene            complement(4946..6988)
                     /locus_tag="VF995_08590"
     CDS             complement(4946..6988)
                     /locus_tag="VF995_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9343655.1"
                     /translation="MNNVLRYTFKKSVKILFTLLVLWAIAIPILYWMYRGARGLDWWN
                     VLGGDPRVDPTWRAYLIKTGHPLAPKFNILKATTAAPSQLLGYAMYLMFFLLIGIVQI
                     LGIFLFLARGRQYTIYPREYDVSFDDVRGQPEIVESTKEALTLFQGFKKFRDAGGYPP
                     HGILFEGPPGTGKTLLGKAIAGSAKVPFLYASGTSFNNMFMGVGNIRIARLFRRARKM
                     SQKYGGAVIFIDELDAVAGSRGAISPTSDRTPADDVRGPMRFIMPGGMGMGSMYVNEL
                     LIQMDGLTMPKGFWRHVKRILHLGKPKIPQYNVMVIGATNQASTLDPAVLRPGRFDRK
                     LHVGLPRADGRMDIIRYYLDKVPHEDVDVARLARATYAFSPAQIKNLVNEALIMALID
                     GREKLNFADLWSAKTTEQFGIKDSRKPSARDRQMTAFHEAGHAVLCYILMTDHQIGIA
                     TIVPRGGTGGHVYPIPLEESFSELSEDKRRWIVMFLGGLAAEEIWFGDTTHGPSSDLR
                     NASSLAAQMLGYFAMGDSVLSYGVLGDNPYTGGAIQAMLEDPHTRDAIRELLADCKRE
                     AVELLRRNEPAVRALAERLLEKEEIYGEEIEELMQAKAVARTTPAFRPVLELIGENWH
                     QPALVDALPRQLEPRRDDGGGSGSQPGAPPATSPQGPGPGPVSDRPTPASSREFEW"
     gene            7233..8858
                     /locus_tag="VF995_08595"
     CDS             7233..8858
                     /locus_tag="VF995_08595"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9343656.1"
                     /translation="MPGLALIARTVWGVAGVAGLEARGGNGAVSGSRHDLAVLVHDGR
                     VAAVVPAVEVAALRPDRVVDLGDATLLPGFVDAHVHLTATGLALGGGVDLRAARSAAD
                     LLEPVRQAAAGLPAGELLWGHGYDESSFLTPALPTAAQLADAAGGRPVYLSRACGHQA
                     LATLDLLERAGALGATGCERDGAGQPTGVVRDDANHQARRHAFGALPARTIVAAQDRA
                     LALAARRGIACVHEMGGPGISSRRDFELLLERGHRLPVEVLCYWGDLDLDFVATRKLA
                     QVGGDLFLDGSLGAHTAALSTPYEDWPEASGSLYHGDADLTDLYVRASLAGVQVAVHA
                     IGDVAIGQALRCARRAARTVGPTAFRALRHRVEHAELLGSDGADRMAELGLAAGVQPG
                     FDAVWGGPGGLYERRLGSRRAKSMNPFADLWRRGVPMGGSSDSNVTPMDPWHGIAAAV
                     HHHRASQRLGLPVALELFTLGGRVLGRQERVTGRIRPGLRADLTAFPGDVLTADPRKL
                     QRREALFTLVRGRLAHGPAELSVESAASAADRA"
     gene            9022..10578
                     /locus_tag="VF995_08600"
     CDS             9022..10578
                     /locus_tag="VF995_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9343657.1"
                     /translation="MATFRVTYSTLSADNEELQAGYEAGLKTARSWLDQTFPAYVNGE
                     ARGDGPAFELSSPNDEHLRLATFHAATERDLDDAITAAKAAFPAWSGMPWQERVAILR
                     RCADVISERTNELAALMSLEVGKNRLEALGDVAESADLIRYYCQQVTDNNGFDRQMGQ
                     LSAGEHTRSVLRPYGVWAVISPFNFPMALAAGPSGGALVAGNTVVLKPSPQGSFTGVK
                     LYECLRDAGLPAGVLHFLPGGDEVGAALVRHPGVDGITFTGSYQVGMEIYHRFAGSYP
                     KPVVCEMGGKNPAIVSANADLDLAAEGVMRAAFGFSGQKCSACSRVYVERPVYDEFTA
                     KLADKARALTVGDPTERAVYMGPVIDRAAVARFEQAVEEARKDGRVLVGGDVLAGDLP
                     DGNYVAPTVVADLPPDHRLFKDELFVPFVAVAAVDSLDEALARANDTEYGLTAGFYST
                     DTDEVRRFLDRIEAGVVYVNRRAGATTGAWPGIQGFGGWKGSGSSGKAGGGPYYVQQF
                     LREQSQTIID"
     gene            10625..>10815
                     /locus_tag="VF995_08605"
     CDS             10625..>10815
                     /locus_tag="VF995_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343658.1"
                     /translation="MAASPQGESPQANKVVSMREAVAELVRDGDTLAIEGFTHLISFA
                     AGHEVIRQRKRDLTLCRLTP"
BASE COUNT         1521 a         4071 c         3728 g         1495 t
ORIGIN      
        1 tagcggcacc tgcttgtcct gggcctcttc aagcacccgg cggttgaact ccaaccagct
       61 cagttcgcgg ttgatataca acgacgggtc atccagggcg gggacgaccg gcgtgatggg
      121 gacagccggg acagccggga cagccgggac agccgggaac ggggcgccga cgacgacttc
      181 atggcgatcg ccaatgccct ccagcttcac cgacaggggc gcaggcgccg cacccgatag
      241 gcgaacggct cgcggaccag ctcgcagcct ggcccggtgg ggcgcataca ctgtcgcggt
      301 ccgaccccgc ccgcaggagc cgtgccgtga ccagcaccga acgccacgac cgccggcctg
      361 gtgagctgct gccctacggg acgccctacc tgatcggcga cccggccgcc gcccgccacg
      421 gcctcaccga gctgcgccgc gccctcgccc acgccggcgt ggtcgccgag gcggccgtcg
      481 cccgccccgc cagcgccagc gccgcgcgcc ggctggccga acgcgccctc aatggcggtg
      541 cccgcctcct ggtcgccgtc ggcgccgatc cgatcgccca tggcctggtc gaggcgctgc
      601 acgcggctga cgctgctcat gaacccgacc gggccctcgg cctggtcggg ctcggacgcc
      661 aagacctcgc cgccaccttc gggttgcccg acgacccgga gcgagcggcc agccacctgc
      721 tcggggtggg ggtgttcctc gccgacctcg ggcgggcacg ctggcagggc ccggacagcg
      781 tccagcgcag cggcgtcttc gccaacagcg ccgaactcgg ctacccggca gaggtctcca
      841 gccgcctgca gcgcgccacc agggcaggcc gcgccggccg tctggccgcc tcggtcaccg
      901 cgctcgcccg cagccacggc acccccgtcc ggctcaccct cgaccacacc accgtcgagt
      961 tccgcctcgc cgggctggtg gtcgccaacg gccagttcac catgggtcgc atgaaggtcg
     1021 ccccgcgcgc cctgcccgac gacggccgca tgaacgtgct cgccttcgaa ggccagccgc
     1081 tcacgatcta cgcccgttcc cgcgagctgt actacggcac ccacctgccc cacccccaga
     1141 tccgcgaata ccagtccaaa ctggttgagg tcgccaccgc cgagccgctc accctcgccc
     1201 tggacggctg ccgggtgcct ggcggcccac cgctggccgt ggacgtgctg cccaaggcgc
     1261 tgcgcatcaa gatctgatca aggcctgaac gatccgcgcg agtttccgtc gtagtctgag
     1321 atcggcaggt tggggcatcg ccgcgaagcg ggcagccagg taggatctgc ccatgactcc
     1381 gacacccact tcgaccgggt acgccgaccg cctggcccgc gcccgccagg agctggtcgc
     1441 ccgcgggatc gacgccctgc tgctcggccc atcggccgac ctggtctacc tcaccggcta
     1501 ctcggcgccg ctgctggagc ggatgacgct gctggtggtg ccggccaatg gcgagccgcg
     1561 cctggtggtg ccggcgcttg aggcgccgct cgccgaggag cagctcgacg acgtgcccct
     1621 ccaggtggcg gcctggcagg agaccgagaa cccggtcgcg ctggtccgtg ccgccctgga
     1681 cgccagcgcc gcgctggacg tcaccggcgc ggccggggga cggctggcgg ttggggaccg
     1741 gctgtggagc accttcctgc tgcggctgca ggtggcgctg ccgacggcca gcttcacggt
     1801 cgcctcgacc atcacccgaa gtctgcgcat gatcaaggac accgaggagc tggacgcgct
     1861 cagccgcgcc gccgaggcga tcgacgaggt ggtcgagcag ctcaccgacc tgcactggat
     1921 cggccgcagc gaacaggacc tggccaggga gctggaccag gcgatccgcg ccgccggtca
     1981 cgaggagacc tgcttcgtga tcgtcgggtc cggacccaac agcgccagcc cccaccacga
     2041 gcccggcgac cgcgtgatcc aggccggcga cgtggtggtg gtcgacatcg gcggccgcct
     2101 cgacggctac tgctcggata cgacccgcac gctggtgatc ggcgagccgc ccgaggggtt
     2161 cctggagttg tatgaggtgc tccgctacgc ccagctcgcc ggctgccagg cggtccgccc
     2221 gggggtgccg gcgtccgccg tcgacaaggc ctgccgggac gtgatcgagc aggccggcta
     2281 cggcagctac ttcatccacc gcaccggcca cggcatcggc ctggaggagc acgaggaccc
     2341 ctacctcgtc accggcaacg acgagccgat cgagccgggc atggccttct cgatcgagcc
     2401 gggcatctac ctgcccggca agttcggcgc ccgcatcgag gacatcgtca tctgcccgga
     2461 cccggacctg gaacctgatc cagacgcgcc cggggcgttc cggctcaacc ggacctcccg
     2521 caagctgcgg gtggtcgacg cctgaccgtg ccatggacgg ccgccggccg agcatgcaag
     2581 cgagcggagg aatgccgttc atgggaggcc ggcggaggcg gaggcggcaa gcctggtgcc
     2641 ctcgctgtga cgagctgcgt cccggccggg ccggcgggca gtgccttgcc tgccgggccc
     2701 ggctggtcgc cctccccggg gtagcggcgc ccgcccccga cccagcccgc gccgccatgg
     2761 cggggcgcct gcgggccctg ctgccagggc tgcgcgcggt cgcgctcacc ctcggcgcgg
     2821 cggcgctggt ggtcggctcc ttcgtggcgg gacggacgac ccggtccagc gccgccgcgc
     2881 agggggccag cggcgtcacg gcggcctcgc catccgccgc cgcgacgacc gcgacgaccc
     2941 agcccgggcc aggggtgcgc ccattcggct ggcacgccag ccacgggcag gtcaccgtca
     3001 ccctgcgcag catcgagaac ggctccgact tcagcaccgt cgtcctggac gtcgccgggc
     3061 tggccggcgg agtcaccgcc ttcaacgggc tgcgcatcga agacgcgcgc ggcaacgacc
     3121 tcaccgggga aggcaccgcc accaaggaac cagtcggcag agatcgcagc gggttccagc
     3181 tgggcggcgg caccgactac ctggtgcagt tcaaccaccc gatcgacttc ggcgcggtgg
     3241 cacgggtgga gctgcacagc ctcaccatcg tccagccgac cgaggagctg gtgtccggaa
     3301 gcctcttcga ccccgcgctg cggcgcagca tcgaccagaa cggccaggcg acagccaccg
     3361 cgacccagcc ctgcccgacc tgccggctcc tgctgcgctg caccacctgc aaggtcaccc
     3421 gcatcctcgg cagcgcctac cggcacggcc gggtggtgct gctgttcacg ccggccgacc
     3481 ccgcccactc gctgatcacc gacgaccaga gctccgaggt cgacgccccc aacgggcagg
     3541 tctccatcga cacgcagccc ctcgccagcg gcggcacgat gatcaccttc caggcgtcga
     3601 gcttggcgct gatctcgccg gccggtcggc ccaccatggc cttcaccacc cacgtgttcg
     3661 ccagccggca gctggtggcc caggggccgt ggcggatcgc ccagaccagc ggcggagcgg
     3721 ggggctagcc gatggcggcc tggtgcccac gctgcgacga gctccgcccc ggcgacggcc
     3781 gcggagcaag cctcagccgc tgcccggagt gcggcgccgc gctggctgac ctggcgcggc
     3841 cacggccggg cggccgcgcc gccgccgctg acccggccgc ggacgcggcg ttcccggtca
     3901 gcctccagcc gccacggtcg cggctgcggg ccgcgctgct ggtgacggcg ctcgccctca
     3961 gcggtcttgg ctacgtcgcc ggccacgccg gcgggcccgg atcagggcca gggtcgggag
     4021 cccgcgccgg tgccggggcg gcctcgtcga ccaccgcatc gacctcgacc agcaccctcc
     4081 ccggaccgtt gcagggcccg gggcggcgcg accagcagct gatcccgcaa cccgagcggc
     4141 gggtgctggg ctggcacgcc agcgacggcc gggccaccct cacgctggaa tcgatcgccc
     4201 gaagcggcgc tcaaaatgac accaacagcg acgccaccgc cgtcctgacc gtccgggtgg
     4261 acgggctgcc agccggccag cacctgctcg gcctgcaggg actcgagctg ctggacgccg
     4321 gtggtggcgt gtatgccacc ccggtggtcg ccagcatcgg caggcagcgc ggagtgaccg
     4381 ccaacctcga cgagagcggc agtggccccg ggaggtacct ggtgtcgctc ggtcccgccc
     4441 cgagcacggc gacgctggcc acggtgcggg tccgggccct gatcgtcagc cggccgccag
     4501 acgtcgccgt gccgctcggc agcgtcccgc ccggcgccgg gccgtcggcg gctctccagg
     4561 cgctgccgga gggcctccac gacaacctcc aggtgccctt ggaagtggcc cctgacctcg
     4621 gccgcaacgc cgccacggtc gagctgtcag ccgccttcgt gagcgcccac cgggcggtgc
     4681 tggtcgccac cgtcgacctg aacggcaccg gcaacttctc cggccaggag gcggtggtag
     4741 cgttcaccag cgggctggtc gccgacggcc gcgaggtctg cgcccgcacc gacgtgttca
     4801 gcgcgagcca gagcgagatc ccctcgatca ccctcgactg cccggccagc ctcacggcgg
     4861 cggcgcggct cagcgcgacc ttcggcagcg ccaccagcac cgtcgccttc cagcggacca
     4921 tcacccgctg accccgcgcg ggccactacc actcgaactc gcggctcgac gccggcgtcg
     4981 ggcggtcgct gaccggcccc ggcccgggac cctgcggcga ggtcgcgggg ggcgctccgg
     5041 gctggctgcc gctgccgccg ccgtcgtcgc gacgaggctc aagctggcgc ggcaacgcgt
     5101 cgaccagtgc cggctggtgc cagttctcgc cgatcagctc gagcaccggg cggaaggccg
     5161 gggtggtcct ggccaccgcc ttggcctgca tcagctcctc gatctcctcg ccgtagatct
     5221 cttccttctc cagcagccgc tcggcaagcg ccctgaccgc cggctcgttg cggcgcagca
     5281 gctccacagc ctcccgcttg cagtccgcca gcagctcgcg gatcgcgtcg cgggtgtgcg
     5341 ggtcctcaag catggcttgg atcgcgccgc cggtgtacgg gttgtcgcca aggaccccgt
     5401 aggagaggac actgtcaccc atcgcgaagt agcccagcat ctgggccgcg agactgctgg
     5461 cgtttctcag gtctgagctg ggaccgtggg tggtgtcgcc gaaccagatc tcctcggctg
     5521 ccagaccgcc caggaacatg acgatccagc ggcgcttgtc ctcgctcagc tcgctgaagc
     5581 tctcctccag cgggatcggg tagacgtgcc cgccggtgcc gccgcgtggg acgatcgtgg
     5641 cgatcccgat ctggtggtcg gtcatcagga tgtagcaaag gaccgcgtgc cctgcctcgt
     5701 ggaacgcggt catctggcgg tcgcgtgccg agggcttcct gctgtccttg atgccgaact
     5761 gctcggtggt cttggccgac cacaggtcgg cgaagttgag cttctcgcgg ccgtcgatca
     5821 gcgccatgat cagcgcctca ttgaccaggt tcttgatctg cgccgggctg aatgcgtagg
     5881 tggcccgggc caggcgcgcg acgtcgacgt cctcgtgcgg caccttgtcc aggtagtacc
     5941 tgatgatgtc catgcggccg tcggccctgg gcaacccgac gtggagcttg cggtcgaagc
     6001 gtcccggccg cagcaccgcc gggtccaggg tggaggcctg gttggtcgcg ccgatcacca
     6061 tcacgttgta ctgcgggatc ttcggcttgc cgaggtgcag gatgcgcttg acgtgccgcc
     6121 agaacccctt cggcatggtc aggccgtcca tctggatcag cagctcgttg acgtacatcg
     6181 agcccatgcc catgcccccc ggcatgatga agcgcatcgg gccgcggaca tcgtctgctg
     6241 gcgtcctgtc tgatgtgggg gagatcgccc cgcggctgcc agccaccgcg tccagctcgt
     6301 cgatgaagat caccgcgccg ccgtacttct gtgacatctt ccgtgcccgg cggaacagcc
     6361 tggcgatccg gatgttgccg acacccatga acatattgtt gaagctggtg cccgaggcgt
     6421 acaggaacgg gactttggcc gagccggcga tcgccttgcc aagcagcgtc ttgccggtcc
     6481 cgggcgggcc ttcgaacagg atgccgtggg gcgggtagcc gccggcgtcg cggaacttct
     6541 tgaagccctg gaacagggtg agggcttcct tggtcgactc gacgatctcg ggctggccgc
     6601 gcacgtcgtc gaaggagacg tcgtactcgc gcgggtagat cgtgtactgc cggccgcgcg
     6661 ccaggaacag gaagatccca aggatctgca cgatcccgat cagcaggaag aacatcaggt
     6721 acatggcgta gccgagcagc tgcgatggcg cggccgtggt cgccttcaga atgttgaact
     6781 tcggcgccag cggatggccg gtcttgatca ggtaggctcg ccaggtcggg tcgacgcgtg
     6841 ggtcgccccc gaggacgttc caccagtcca ggccgcgcgc accccggtac atccagtaca
     6901 ggatcgggat cgcgatcgcc caaaggacga ggagcgtgaa caggatcttg acggatttct
     6961 tgaaggtgta gcgtagtacg ttattcaagg aagccccgcc ttcccaagcc gccgccagtc
     7021 gtctccccgc cccagcagta tgcgccgagc ctcgttgaac tgctacagcc gtacgagcgc
     7081 cgggcagaac gggcggcttg ctgtctaccc gtttgcccgc cctgcgcctt tacagcgcaa
     7141 cgcccctgcc tccatattgt cgtggccgcc cggcgctaaa cctatcgacc tatcggcagg
     7201 ttgcggacgg ttcgaaagga ggagcttcga ccgtgcccgg cctcgcgttg atcgcccgga
     7261 ctgtctgggg cgtggcgggt gtggccggcc tcgaagcgcg cggcggcaac ggcgcggtct
     7321 ccggcagccg ccacgacctc gccgtgctgg tccacgacgg gcgcgtcgcg gcggtcgtgc
     7381 ccgccgtcga ggtcgccgcg ctgcggcccg accgggtcgt cgatctcggc gacgcgaccc
     7441 tgctgcccgg gttcgtcgac gcgcacgtgc acctgaccgc caccgggctc gccctcggcg
     7501 gcggcgtgga cctgcgcgcc gcccgctcgg ccgccgacct gctcgaaccg gtccggcagg
     7561 ccgccgccgg cctgccggcg ggggagctgc tgtggggcca cggctacgac gagagcagct
     7621 tcctcacccc ggcgctgccg accgcggcgc agctggccga cgccgccggc gggcgccccg
     7681 tgtacctgtc gcgcgcctgc ggccaccagg cgctggcaac cctcgacctg ctggagcgcg
     7741 ccggcgcgct gggcgccacc gggtgcgagc gcgacggcgc cgggcagccc accggggtgg
     7801 tgcgcgacga cgccaaccac caggcccgcc gccacgcctt cggggcgctg ccggcacgga
     7861 ccatcgtcgc cgcccaggac cgtgccctgg cgctggccgc ccggcgcggg atcgcctgcg
     7921 tccacgagat gggcggcccc ggcatctcca gccggcggga cttcgagctg ctgctggagc
     7981 gcggccaccg cctcccggtc gaggtgctct gctactgggg cgacctggac ctcgacttcg
     8041 tcgccacacg caagctcgcc caggtcggcg gcgacctgtt cctggacggg tcgcttggcg
     8101 cccacaccgc cgcgttgtcg accccctacg aggactggcc ggaggccagc ggctcgctct
     8161 accacggcga cgccgacctc accgacctgt acgtccgcgc cagcctggcc ggcgtccagg
     8221 tggccgtcca cgccatcggc gacgtcgcca tcggccaggc gctgcgttgc gcccgccggg
     8281 cggcgcggac ggtcgggccg accgccttcc gggcgctgcg ccatcgggtc gagcatgccg
     8341 agctgctcgg ctccgacggc gccgaccgca tggccgagct gggcctggcg gccggggtgc
     8401 agcccgggtt cgacgcggtc tggggcggcc ccggcggcct ctacgagcgc cgtctcggca
     8461 gccgccgggc caaaagcatg aacccgttcg cggacctgtg gcgccgcggc gtgccgatgg
     8521 gcgggtcgtc ggactcgaac gtcaccccga tggatccctg gcacggcatc gccgccgccg
     8581 tccatcatca ccgcgccagc cagcggctcg gcctgccggt ggcgctggaa ctgttcaccc
     8641 tcggcggccg ggtcctcggc cgccaggagc gggtcaccgg gcggatccgg ccgggcctgc
     8701 gcgccgacct gaccgccttc cccggcgacg tcctgactgc cgacccgcgg aagctgcagc
     8761 gccgcgaggc gctgttcacc ctggtcagag gccggctcgc ccatggcccc gcggagcttt
     8821 cggtggagtc ggcggccagc gccgccgacc gcgcctgagc ggccgccccg cgccggcctg
     8881 cctgccctcc tgcctgccct gtggataacc agaaccgggt ggcggcgagg tcactacact
     8941 catgcagagc ccgccgtgcg gggtgggggg tacagggccg accgaacgac agcgacagcg
     9001 acagcgaagg agttggagga catggccacg tttcgcgtca cctattccac gttgtccgcc
     9061 gacaacgagg agttgcaggc cggctacgaa gccggactga aaaccgctcg gtcctggctc
     9121 gaccagacat tcccggcata cgtcaacggc gaggctcgcg gcgacggccc cgccttcgag
     9181 ctgagcagcc ccaacgacga gcacctccgg ctcgccacct tccacgccgc caccgaacgc
     9241 gacctcgacg acgcgatcac cgctgccaag gccgccttcc ccgcatggtc cgggatgccc
     9301 tggcaggaac gagtggcgat cctgcgccgc tgcgccgacg tcatcagcga gcgcaccaac
     9361 gagctcgccg ccctgatgag cctcgaggtc ggcaagaacc gccttgaagc cctcggcgac
     9421 gtcgccgagt ccgccgacct gatccgctac tactgccagc aggtcaccga caacaacggc
     9481 ttcgaccgcc agatgggcca gctctccgcc ggtgagcaca cccgcagcgt gctgcggccc
     9541 tacggcgtct gggcggtgat cagcccgttc aacttcccga tggcgctggc cgccgggcca
     9601 tcgggcggcg cgctggtcgc tggcaacacg gtggtgctca agccctcccc gcagggctcc
     9661 ttcaccggcg tcaagctcta cgaatgccta cgcgacgccg gcctgcccgc aggggtcctc
     9721 catttcctgc ccggcgggga tgaggtcggc gcggcgctgg tgcgccaccc cggcgtcgac
     9781 gggatcacct tcaccggctc ctaccaggtc ggcatggaga tctaccatcg cttcgccggc
     9841 agctacccca agccggtcgt ctgcgagatg ggcggcaaga accccgcgat cgtctccgcc
     9901 aacgccgacc tcgacctggc tgccgaaggg gtcatgcggg cggcgttcgg gttcagcggc
     9961 cagaaatgct cggcctgctc gcgggtgtac gtggaacggc ccgtctacga cgagttcacc
    10021 gccaagctcg ccgacaaggc ccgcgcgctg accgtcggcg acccgaccga gcgtgccgtc
    10081 tacatgggtc cggtgatcga ccgcgcggcc gtcgcccgct tcgagcaggc cgtcgaggag
    10141 gctcgcaagg acggccgcgt cctcgtcggc ggcgacgtcc tggcaggcga cctgcctgac
    10201 ggcaattacg tcgccccgac cgtggtcgcc gatctgccac ccgaccaccg gctcttcaag
    10261 gacgagctgt tcgtgccgtt cgtggcggtc gccgcggtgg actccctcga cgaggcgctg
    10321 gcacgcgcca acgacaccga atacggcctc accgccgggt tctactccac cgacaccgac
    10381 gaggtccggc gtttcctcga ccggatcgag gcaggggtgg tctacgtcaa ccgccgcgcc
    10441 ggcgccacca ccggcgcctg gccgggaatc caggggttcg gcggctggaa gggctccggc
    10501 agctccggca aggccggcgg cggcccctac tacgtgcagc agttcctccg cgagcagtcg
    10561 cagacgatca tcgactgaaa ccgactggtc aagctgaccg agggaggtgc cggtggtcga
    10621 cgacatggcc gccagcccgc agggggagag cccccaggcg aacaaggtcg tcagcatgcg
    10681 tgaggcggtc gccgagctgg tccgcgacgg cgacacgctg gcgatcgagg ggttcaccca
    10741 cctgatctcg ttcgccgcgg ggcatgaggt gatccgccag cgcaaacgcg acctcaccct
    10801 gtgccggctc acccc
//
LOCUS       DASWDC010000266         5232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_121337, whole genome shotgun sequence.
ACCESSION   DASWDC010000266 DASWDC010000000
VERSION     DASWDC010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121337"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VF995_08610"
     CDS             <1..1338
                     /locus_tag="VF995_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX9343659.1"
                     /translation="GPESPGRAVVRRCAPTQAILLDGPCCVVRYELELGDGSGRVVPA
                     LVSGRLFTEPATAARYAAERLAPLAERVRGRAEVAPFARAAAVIEPLGMAVHAFPVDG
                     ELPTLVDATDPGVAARALGELLAASGHGQAAAPGQVAGCRVEPVHYNRRRRCMLRYRL
                     DLAGGGQLVLYGKVSNDGAGERTPAVVAALRDALDRAGTRFTVPECLGFRPDLQLVVL
                     TEIPGVPQVAQLLKARLRGEPAPGPAADGLTLEDAVSACGLIAAVLHTSGIQLGVERP
                     LERELADLRAEVAPIRRVSPELGGRLGRWLDLAEAHAAPTAALPLCQSHGDFSYTQLI
                     FHGSQSGLVDFDNFCQAEPALDLGHFMAYLRFAGVKARGMSPTERAALGQDLAARFTA
                     AYTGAGGPAEALARVPVYETVSLVRLAVHAWQRFKGNRLGNVLTVLDERLLDT"
     gene            1368..2780
                     /locus_tag="VF995_08615"
     CDS             1368..2780
                     /locus_tag="VF995_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343660.1"
                     /translation="MWEGLGRFAAMPPWLAALADPARVEAALRPAVPGLRHATLGEVR
                     LKRKAWTARCELAVAASGSDGRPGGQPGGPAVDQAGERLVHRVATIIPPRAHDKDDGD
                     DEAGEAAARLVLDPDEQVEAPLPALALLTDPERARELLERAIRAGTPAYRELRILGVT
                     PRVMRYTPGSRCTIRYRLELAPGSPGPGVVVAKTYHRSGKGRIAWEGMGALWDSPLAR
                     SEVVAVAAPLAWLPELNVLVQGPVREERTLKDLLLEALTTGDEANLGVLRAQLARTAA
                     GLAELHRCGAPSAQLVTWDDELAEVRQVLDRLAPRVPELEGAADPFLDDLRRFAARVP
                     ADPALPAHRSFRPAQVLLYHDEIAFIDFDGFCHAEPALDLALFRATIRDLGIGAFPPG
                     VPTETRLDRLDQLCDEFLAAYERLAPVSRERVALWEALDLFTNVLHSWTKIRPARLAH
                     ATTLLQHHLAGLVAAFAPQM"
     gene            2857..3909
                     /locus_tag="VF995_08620"
     CDS             2857..3909
                     /locus_tag="VF995_08620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX9343661.1"
                     /translation="MGPDPARRDLPGEFGLAVENFTPARAQPSIERILAYAGAAESLG
                     YRSLWVWDHLFLGKRRPFPFLEALSTLAALSTRTSAAALGTGVLVLPLRDPVLLAKTA
                     ATVDLLSGGRLALGVAAGWYEREFQACGVPFKQRGRLFERNLEILDRLWREPEVSGTW
                     DELDLRGVAMRPPPASRPRPPILIGGYVDRVLRRVATRGDGWLTYFYTPESFRRSWTR
                     IGEFAVEAGRDPATFTNVAQVPLCVGASYEEADRRVRAFVAEYFDTPAWSESTPDSAI
                     RGTPAQCAEQVAAYRAAGVRHLCLVPDQYQVEQAERFATEVLPLLGPLLGHGQQGQTG
                     PPPGPGATAEAAEPTT"
     gene            3906..4841
                     /locus_tag="VF995_08625"
     CDS             3906..4841
                     /locus_tag="VF995_08625"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX9343662.1"
                     /translation="MTAPPPVRLRPVADKVLPAADAVALVRDGDVVAAGGTLYSRTPM
                     ALLFALLRRRPRPRGLALVRPLTCYEAELFLVAGAAERLITSWVGIGIPWGVSRVLRE
                     LVGGGRATYEEWSHLALGLRLKAGAMGVPFLPTVTMLGSDLARPGDTHELACPFTGEK
                     VLLVPALNPDVALLHVHRADRFGNAQIDGYSHMDADMATAARTVIVSAEEVVAPEVIR
                     RRPDATVLPHFLVDAVVEAPMGAFPHECYGRYEADFAHFDEYAAAIRQDGLAAVSTYL
                     DRHVHGQPDFAAFLASFGATRLQRQRERAAELVHQ"
     gene            4838..>5232
                     /locus_tag="VF995_08630"
     CDS             4838..>5232
                     /locus_tag="VF995_08630"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX9343663.1"
                     /translation="MSGTAGATPYRPSYTAQELMAVSASRLLEDHRVVFAGVGLPLLA
                     SILAKRRHAPELTIVLEGGIIGPGILPGRLPISTNEMRAAHQATMLTPISEIFLLAQR
                     GCFHYGFLGAAQIDMFGNINTSVIGPAGRP"
BASE COUNT          626 a         2036 c         1864 g          706 t
ORIGIN      
        1 ggcccggaat ccccgggtcg tgccgtggtg cggcgctgcg cgcccaccca ggcgatcctc
       61 ctcgacggcc cctgctgcgt ggtccgctac gagctggagc tgggggacgg ctcggggcgg
      121 gtggtgcccg cgctggtcag cggccgcctc ttcaccgagc ccgccaccgc ggcccgctac
      181 gccgccgagc ggctcgcccc gctcgccgag cgggtgcggg gtcgcgcgga ggtggccccg
      241 ttcgcccgcg ccgccgcggt catcgagccg ctcggcatgg cggtccacgc cttcccggtc
      301 gacggtgagc tgcccacgct ggtcgacgcg accgatcccg gagtggccgc acgcgccctc
      361 ggcgagctgc tggccgccag cgggcacggg caggcggccg cgcccggcca ggtcgccggc
      421 tgccgggtcg agccggtcca ctacaaccgc cgccgccgct gcatgctccg ctaccgcctg
      481 gacctggccg ggggagggca gctggtcctc tacggcaagg tcagcaacga cggcgccggg
      541 gagcgcaccc cggcggtcgt cgccgccctc cgcgacgccc tcgaccgggc ggggacgcgc
      601 ttcaccgtcc ccgaatgcct cggcttccgc cccgacctgc agctggtcgt gctgaccgag
      661 atccccggcg tcccccaggt cgcgcagctc ctcaaggcac gcctgcgggg cgagcccgcc
      721 ccaggcccag cggcggacgg gctgaccctg gaggacgccg tcagcgcctg cgggctgatc
      781 gcggcggtgc tgcacacctc gggaatccag ctgggcgtgg agcggccgct ggagcgcgag
      841 ctggcggacc tgcgagcgga ggtcgcgccg atccggcggg tctcgccgga gctgggcggg
      901 cgcctcggcc gctggctcga cctggccgag gcgcacgccg cccccaccgc ggcgctgccc
      961 ctgtgccaga gccatggcga cttcagctac acccagctca tcttccacgg ctcccagagc
     1021 gggctggtgg acttcgacaa cttctgccag gccgagccag cgctcgacct cggccacttc
     1081 atggcctacc tgcgcttcgc gggggtcaag gcacggggca tgtcgcccac ggagcgggcc
     1141 gccctcggcc aggacctcgc cgcccgcttc accgccgcct acaccggcgc cggcggtccc
     1201 gccgaggcgc tcgcccgggt gccggtgtac gagacggtca gcctcgtccg gctggcggtg
     1261 cacgcctggc agcgcttcaa gggcaaccgg ctcggcaacg tcctgaccgt gctggacgag
     1321 cgcctgcttg acacctgaca cctcgacgtc gagctgaggg ggaacgtatg tgggaggggc
     1381 tgggccgctt cgccgccatg ccgccctggc tggccgcgct cgccgacccc gcgcgggtcg
     1441 aggcggcgct gcgcccggcc gtccccgggc tgcggcacgc caccctcggc gaggtgcgcc
     1501 tgaaacgcaa ggcatggacc gcccgctgcg agctggccgt ggccgccagc ggcagcgacg
     1561 gccgccccgg tgggcaaccc ggcggccccg ccgtggacca ggccggcgag cgcctggtcc
     1621 accgggtcgc cacgatcatc ccaccacgcg ctcacgacaa agacgacgga gacgacgaag
     1681 ctggcgaggc cgcggcgcgg ctggtcctgg acccggacga gcaggtggag gcgccgttgc
     1741 cggcgctggc gctgctgacc gacccggagc gcgcccgcga gctgctggag cgggccatcc
     1801 gcgccggcac gcccgcctac cgggagctgc gcatcctcgg cgtcacaccc agggtgatgc
     1861 ggtatacccc ggggagccgc tgcaccatcc gctaccgcct ggagctggct cccgggtcgc
     1921 ccgggcccgg tgtggtggtc gccaagacct atcaccgctc cggcaagggc cggatcgcct
     1981 gggaggggat gggggcgctg tgggactcgc cactggcccg ctccgaggtg gtcgccgtcg
     2041 ccgcgccgct cgcctggctg ccggagctga acgtgctggt ccagggcccg gtccgcgagg
     2101 agcggacgct caaggatctg ctgctggagg cgctcaccac cggcgacgag gccaacctcg
     2161 gcgtcctgcg cgcccagctg gccaggaccg cggcgggcct ggccgagctg caccgctgcg
     2221 gcgctccgag cgcgcagctg gtcacctggg acgacgagct ggccgaggtg cgccaggtcc
     2281 tcgaccggct cgccccgcgt gtccccgagc tggagggcgc ggccgatccc ttcctcgacg
     2341 acctgcgccg cttcgccgcc cgcgtccccg ccgacccggc gctcccagct caccgcagct
     2401 tccgtcccgc gcaggtgctg ctctaccacg acgagatcgc cttcatcgac ttcgacggct
     2461 tctgccatgc cgagcctgcc ctggacctgg cgctgttccg ggccaccatc cgcgacctcg
     2521 gtatcggcgc gttcccgccg ggggtcccga ccgagacccg cctcgaccgc ctcgaccagc
     2581 tctgcgacga attcctcgcc gcctacgagc gcctcgcgcc ggtctcgcgc gagcgggtcg
     2641 cgctgtggga ggcgctggac ctgttcacca acgtcctgca ctcctggacc aagatccggc
     2701 ccgcccggct ggcccatgcc accacgctgc tccagcacca tctcgccggc cttgttgcgg
     2761 cgttcgcacc ccagatgtga tcagcttgat caactggtcc taccatcagg ccagttgatc
     2821 actgcttttg gacggcggca gcggcgagga gcggcgatgg gtcctgaccc cgcacggcgt
     2881 gacctgcccg gcgagttcgg gctggcggtc gagaacttca cccccgcgag ggcgcagccg
     2941 agcatcgagc ggatcctcgc ctacgccggc gccgccgagt cgctcggcta ccggtcgctg
     3001 tgggtctggg accacctgtt cctcggcaaa cgccggccgt tcccgttcct ggaggcgctg
     3061 tccaccctgg cggcgctgtc cactcgcacg tcggcggcgg ccctcgggac cggcgtgctg
     3121 gtgctgccgc tgcgcgaccc ggtgctgctc gccaagaccg ccgcgaccgt ggacctgctg
     3181 tcgggcgggc ggctcgccct cggcgtcgcc gccggctggt acgagcgcga gttccaggcc
     3241 tgcggcgtgc ccttcaagca gcgcgggcgg ctgttcgagc gcaacctcga gatcctcgac
     3301 cgcctctggc gcgaaccgga ggtcagcggc acctgggacg agctggacct gcgtggcgtg
     3361 gccatgcggc cgccgccggc cagccggccc cgccctccca tcctgatcgg cggctacgtc
     3421 gaccgggtgc tgcgccgcgt cgccacccgc ggcgacggct ggctgaccta cttctacacg
     3481 cccgagagct tccgccggtc gtggacgcgg atcggcgagt tcgccgtcga ggccggccgc
     3541 gaccccgcca ccttcaccaa cgtcgcccag gtgccgctct gcgtcggcgc ctcctacgag
     3601 gaggccgacc gtcgcgtgcg cgccttcgtc gccgagtact tcgacacccc cgcctggagc
     3661 gagtccaccc ccgacagcgc catccgcggc acccccgcgc agtgcgccga gcaggtcgcc
     3721 gcctaccggg ccgccggcgt gcggcacctc tgcctggtac ccgaccagta ccaggtcgag
     3781 caggccgagc ggttcgccac cgaggtcctg cccctgctcg gccccctgct cggccacggc
     3841 cagcagggcc agaccgggcc gccgcccgga cccggggcga cggccgaggc ggcggagccg
     3901 accacatgac ggctccaccc ccggtccggc tgcggccggt ggccgacaag gtgctgcccg
     3961 ccgccgacgc ggtggcgctg gtgcgggacg gggacgtcgt cgcggcgggc gggacgctgt
     4021 actcccgcac gccgatggcg ctgctgttcg cactgctgcg ccgccggccc cgtccccgcg
     4081 gcctcgccct ggtccgcccg ctgacctgct atgaggccga gctgttcctg gtcgcgggtg
     4141 cggccgagcg gctgatcacc agctgggtcg ggatcggcat cccgtggggg gtgtcgcggg
     4201 tgctgcgcga gctggtcggc ggcgggcggg cgacctacga ggagtggagc cacctggcgc
     4261 tggggctccg cctcaaggcg ggcgccatgg gggtgccgtt cctgcccacc gtgacgatgc
     4321 tcggttccga cctcgcccgc ccgggcgaca cccacgagct ggcctgcccg ttcaccggcg
     4381 agaaggtgct gctggtcccg gctctcaacc cggacgtggc cctgctgcac gtgcaccgcg
     4441 ccgaccgctt cggcaacgcc cagatcgacg gctacagcca catggacgcc gacatggcca
     4501 cggcggcccg gacggtgatc gtgagcgccg aggaggtcgt cgcgcccgag gtcatccggc
     4561 ggcgccccga cgccaccgtg ctgccgcact tcctggtcga cgcggtggtc gaggcgccga
     4621 tgggggcctt cccgcacgag tgctacggcc gctatgaggc ggatttcgcc catttcgacg
     4681 aatacgcggc agcgatccgg caggacggcc tcgccgcggt ctccacctac ctggaccgcc
     4741 acgtccacgg gcagccggac ttcgccgcgt tcctcgccag cttcggcgcg acgcgcctgc
     4801 agcgccagcg ggagcgggcc gcggagctgg tgcaccagtg agcggcactg ctggcgcgac
     4861 gccgtatcgg ccgtcgtata ccgcccagga gctgatggcg gtctcggcca gccgcctgct
     4921 ggaggaccat cgggtggtgt tcgccggggt cggcctgccg ctgctggcct cgatcctggc
     4981 gaagcggcgg cacgcgccgg agctgaccat cgtgctcgaa ggcggcatca tcgggccggg
     5041 gatcctgccc ggcaggctgc cgatctccac caacgagatg cgagcggccc accaggcaac
     5101 catgctgact ccgatcagcg agatcttcct gctggcacag cgcggctgct tccactacgg
     5161 cttcctcggc gccgcccaga tcgacatgtt cggcaacatc aacaccagcg tgatcgggcc
     5221 ggccgggcgg cc
//
LOCUS       DASWDC010000267         4129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_74913, whole genome shotgun sequence.
ACCESSION   DASWDC010000267 DASWDC010000000
VERSION     DASWDC010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74913"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3111
                     /locus_tag="VF995_08635"
     CDS             <1..3111
                     /locus_tag="VF995_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9343664.1"
                     /translation="RLHIAIATGEALVRHDAARRGGQRVLGDLVTTCAHLQEATPTGA
                     IMVTDATMQATERTISYGPASIVALRNGEPVTVWSALEPRAKMPPGTRPRRLPPLVAR
                     MRELGALLERFAFARAGGTPQLVVVTGPPGIGKSRLLTEFSRVAEAGIDPAVFRHGRS
                     LAYNDGMRALSEIVETEAGILETDSADEAERKLEWAVFNAIDDPAAAVWVNRHLRRLL
                     GTSAEGQLGAGDREEMLVAWRHYLYGRAQQKPLVLAMEDLHWADDTLLDFIESVVDPE
                     IAAQAGQVPLIMVAAARPELLDRRPSWLSGRPGITVVPVEPLAEADTSRLVTALLSHH
                     GLDGGPGEGTAGGDTAPDPALLARIAGNPLFAEEYVRMLRDRGGTATLDDPGQLPVEG
                     APGTVTDAPVRPAPAPPPMPTTVHAIIAARLDALPADEKALIHDAAVIGHVGWVGALA
                     RVGGGEPDADREAVETLLTRLERKDFLLRAERSRVAGETEYRFHHLLVRDVAYGQIVR
                     AERAAKHQRAAAWIEAIVTDRAAEHAELLAHHYHSAYTLNRAAGIEAPGLAAKAMRAS
                     RDAGDRVAALGIYAGANRYYSEALALCTAADPGRAELLLRLGRARCAGEGGGGDVLRE
                     AHALLLELADRTAAAEAQMLLAEVAFLHGSGEERAGHVNQALALIVDAPPSPGKAAVL
                     KGCMLHLVIANRHAEALEVARQVRGMARGLGLRDLEGSALGTIGLARVDAGDPGGIVD
                     LENAITIFEQLGTPGAIIWHMNLAYARATLGDLPGCFAALAAGSEMAERFGSLRWIRA
                     MRLQRVAEYYWTGEWDEAVTIVNALVAESGAGNGQARHYLEWECRLWRGRIRLARGQV
                     AAALDDAEAALLVATEVADPQALNPTRTFRARALLAAGRDDEAHALATDLLTGLGGSL
                     LGPELGVDLGVVLASLDLLDGSPAEAESPLAGLGVPPSRWRDAIDAFVRGGAARAADL
                     YATIGSAPDEAHARLEAARDALATGHTPEGEDQLSSALAFYRQVAASAYLVEAEALES
                     TLVREP"
     gene            3334..>4129
                     /locus_tag="VF995_08640"
     CDS             3334..>4129
                     /locus_tag="VF995_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343665.1"
                     /translation="MMQLPVAGISQERRQARAELATEVELGVRTRHGDLRKALAELER
                     QGPQDSPVRHQLALAAVHLDLGEVQKARGQEPTELWNADSRVTTAMRLADRAPDEVRA
                     AAQLEAYGFAALYANRSDVGLRLNVLPHLDRAGQALTELHPEWVTTSAYGDRVAPLLQ
                     GLHRLETQLAERLLDRGDPQAKFWIDPLGTPPEGHPLWPYHQRVKLLAGIRFGGELAG
                     EAALDKTTAIAHAFSEQDQPAEAGKTYLSMTGVFLGEASRYRTKGPL"
BASE COUNT          574 a         1692 c         1404 g          459 t
ORIGIN      
        1 cgcctccaca tcgccatcgc caccggcgag gcgctggtgc gccacgacgc cgcccggcgc
       61 ggggggcagc gcgtcctcgg cgacctggtc accacctgcg cccacctgca ggaggcgacc
      121 ccgaccggcg cgatcatggt caccgacgcc accatgcagg cgaccgaacg gaccatctcc
      181 tacggccccg ccagcatcgt cgccctgcgc aacggcgaac cggtcaccgt ctggagcgcc
      241 ctggaacccc gcgccaaaat gccgcccggc acccgcccgc gccgcctgcc gcctctggtc
      301 gcccgcatgc gcgagctggg cgcgctgctg gagcgcttcg ccttcgcccg cgccggcggc
      361 accccgcagc tcgtcgtcgt caccggcccg ccggggattg gcaagagccg cctgctcacc
      421 gagttctccc gcgtcgccga ggcgggcatc gaccccgccg tcttccgcca cggccgcagc
      481 ctcgcctaca acgacggcat gcgcgccctc tccgagatcg tcgagaccga ggccggcatc
      541 ctggagaccg actccgccga cgaggccgag cgcaagctcg aatgggccgt cttcaacgcc
      601 atcgacgacc cggccgccgc cgtctgggtc aaccgccacc tccgccgcct gctcggcacc
      661 agcgccgaag gccagctcgg cgcgggcgac cgcgaggaga tgctggtcgc ctggcgccac
      721 tacctctacg gccgcgccca gcagaagccg ctggtgctcg ccatggagga cctccactgg
      781 gccgacgaca ccctgctcga cttcatcgaa tccgtggtcg accccgagat cgccgcccag
      841 gccgggcagg tcccgctgat catggtcgcc gccgcccgcc cggagctgct ggaccgccgc
      901 ccctcgtggc tctcgggccg cccggggatc accgtcgtcc cggtcgagcc gctcgccgag
      961 gccgacacca gccgcctggt caccgcgctc ctcagccacc acggcctcga cggcggcccg
     1021 ggcgagggca ccgcgggcgg cgacaccgct cccgaccccg ccctcctggc acggatcgcc
     1081 ggcaacccgc tgttcgccga ggaatacgtc cgcatgctgc gcgaccgcgg cggcaccgcc
     1141 accctcgacg accccggcca gctcccggtg gagggcgccc cgggcacggt caccgacgcg
     1201 ccggtgcgcc ccgcccccgc tcccccgccg atgccgacga ccgtccacgc gatcatcgcc
     1261 gcccgcctcg acgcgctgcc ggccgacgag aaggccctga tccacgacgc cgccgtgatc
     1321 ggccacgtcg gctgggtcgg cgcgctcgcc agggtcggcg gcggcgaacc cgacgccgac
     1381 cgggaagccg tcgagacgct gctgacccgc ctggagcgca aggacttcct gctgcgcgcc
     1441 gagcgctcgc gcgtggccgg cgagaccgag taccgcttcc atcatctgct ggtccgcgac
     1501 gtcgcctacg gccagatcgt gcgtgccgag cgggccgcca agcaccagcg cgccgccgcc
     1561 tggatcgagg cgatcgtcac cgaccgggcc gccgagcacg ccgagctgct cgcccaccac
     1621 taccactccg cctacacctt gaaccgcgcc gccgggatcg aggcgccggg cctcgccgcc
     1681 aaggcgatgc gcgcctcccg cgacgcgggc gaccgggtcg cggccctggg gatctacgcg
     1741 ggcgcgaacc gctactactc cgaggcgctc gccctctgca cggcggccga cccgggccgc
     1801 gcggagctgc tgctgcgcct cggccgggcg cgctgcgccg gcgagggcgg cggcggcgac
     1861 gtgctccgcg aggcccacgc cctcctgctg gaactggccg accggaccgc tgccgccgag
     1921 gcgcagatgc tgctcgccga ggtcgccttc ctgcacggca gcggcgagga gcgcgccggg
     1981 cacgtcaacc aggccctcgc gctgatcgtc gacgcgcccc catcgcccgg caaggccgcc
     2041 gtgctgaagg gctgcatgct gcacctggtg atcgccaacc gccacgccga ggcgctggag
     2101 gtcgcccgcc aggtgcgcgg catggcccgc gggctcggcc tccgcgacct ggagggcagc
     2161 gccctcggca ccatcggcct cgcccgggtg gacgcgggcg accccggcgg gatcgtggac
     2221 ctggagaacg ccatcaccat cttcgagcag ctcggcaccc ccggcgcgat catctggcac
     2281 atgaacctcg cctacgcccg cgccaccctc ggggacctgc cgggctgctt cgcggcgctg
     2341 gcggccgggt ccgagatggc cgagcgcttc ggctcgctgc gctggatccg cgcgatgcgg
     2401 ctgcagcggg tcgccgagta ctactggacc ggcgagtggg acgaggccgt cacgatcgtc
     2461 aacgcgctgg tcgccgaatc gggcgccggg aacggccagg cccgccacta cctggaatgg
     2521 gaatgccgcc tgtggcgcgg gcgcatccgg ttggcccgcg gccaggtcgc cgccgccctg
     2581 gacgacgccg aagccgccct cctggtggcc accgaggtcg ccgacccgca ggccctcaac
     2641 ccgacccgca ccttccgcgc ccgtgcgctg ctcgccgccg ggcgcgacga cgaggcccac
     2701 gcgctggcca ccgacctgct caccggcctc ggcggcagcc tcctgggtcc cgagctgggc
     2761 gtcgacctcg gcgtggtgct cgccagcctg gacctgctcg acggcagccc ggcggaggcg
     2821 gagtcgccgc tggccgggct cggcgtcccg ccctcccgct ggcgcgacgc gatcgacgcc
     2881 ttcgtgcgcg gcggcgccgc ccgcgccgcc gacctctacg ccaccatcgg ctccgccccc
     2941 gacgaggccc acgcccgcct ggaggccgcc cgcgacgccc tcgccaccgg ccacaccccc
     3001 gaaggcgaag accagctcag ctccgccctc gccttctacc gccaggtcgc cgccagcgcc
     3061 tacctggtcg aagccgaagc cctcgaatcc accctggtcc gcgaaccctg aggcgaaccc
     3121 tgaggcgatc ccggagcccg ctccgggtcg cgttccagcc tccggccgcg catagcgggg
     3181 acgaacggca gcgccacgcg gcacggggag gagcgagttg gcgggacccg atcaccccgg
     3241 gcccgggccc ggacccggga acccggacgg tccccagcga ggagacccgg agcagcaacg
     3301 gcatcagacg cgcgccgcag cgacgagcca accatgatgc agctccccgt cgcggggatc
     3361 tcgcaggagc gccgccaggc ccgcgccgag ctcgcaacgg aggtcgagct cggcgtccgg
     3421 acccgccacg gcgacctccg aaaagcgctg gccgagctcg aacgtcaggg cccccaggac
     3481 tcccccgttc ggcatcagct ggcgctggcg gccgtccacc tggacctcgg cgaggtccag
     3541 aaggcccgcg ggcaggagcc gaccgagctc tggaacgcgg acagccgggt gacaacggcg
     3601 atgaggctgg cggaccgcgc gcccgacgag gtcagagccg ccgcgcaact ggaggcctac
     3661 gggttcgccg cgctgtacgc caaccgtagt gacgtcgggc tgcgcctcaa cgtcctgccc
     3721 cacctcgacc gcgccggtca agcgctcacg gagctgcacc cggagtgggt gacgaccagc
     3781 gcctacggcg accgggtagc gcccctcctg caggggctgc acaggctcga gacgcagctc
     3841 gccgagcggc tcctggaccg gggcgacccg caggccaagt tctggatcga cccgctcgga
     3901 accccgccgg aaggtcatcc gctgtggcca taccatcagc gcgtcaagct cctggcgggg
     3961 atccggttcg gcggggagct ggccggcgaa gccgcgctcg acaagaccac ggcgatcgcg
     4021 catgccttct ccgagcagga ccaaccggcc gaagcaggca agacctacct gagcatgacc
     4081 ggcgtgttcc tgggtgaggc gtccagatac cgcacaaagg gccccctcg
//
LOCUS       DASWDC010000268         7667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1242988, whole genome shotgun sequence.
ACCESSION   DASWDC010000268 DASWDC010000000
VERSION     DASWDC010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242988"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VF995_08645"
     CDS             <1..617
                     /locus_tag="VF995_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9343666.1"
                     /translation="AYPGPGERVARLEETLKLLRALLADAEAPTSFEGRFYHTHDAPV
                     IPGPVQQPWPPLWVGGRGDRMLGVTARAADGWNYCWSATPEQYGERLEVLARACARAG
                     RPFGAVRRSLGLSTLVGRDPDDLVARWRRLRAWAPGGALDTVELADWAASRLVGTPDE
                     VVAQLRGWQARGIEQVICSFANVPFAIFEDDQLDLVAELVLPKL"
     gene            complement(629..1447)
                     /locus_tag="VF995_08650"
     CDS             complement(629..1447)
                     /locus_tag="VF995_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9343667.1"
                     /translation="MREREVEGAGGSIVFDRAAGYYDDTRGLPAEVSDQVTDRIEAAA
                     GPDARLLELGVGTGRIALPLQRRGRTVFGVDLSLPMLERYRANAAAAGLPRPPLLRGD
                     VSRLPVRDGGVGAVVEVHVLHLIPAWRQVLAEARRVLAPGGVLLMGKRGSVSHGGGPW
                     RETRRRWRALVEGLGGHPKRPGVQTPEEVAAAITDLGGQAEELPPVAWQVDETWGESL
                     RLMEQRLFSDTWLVGDAAWQASLGPLCAEIEASGVDLDAPVPVTISFELLAVRF"
     gene            1492..1728
                     /locus_tag="VF995_08655"
     CDS             1492..1728
                     /locus_tag="VF995_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343668.1"
                     /translation="MEPREACLKVLDDAAPEPLHWTVVLDRALRERLLDPFTVHDVRG
                     AVVKNLAALAKEGRAVKVGTGLYRAAGTQPDGSA"
     gene            complement(1771..2421)
                     /locus_tag="VF995_08660"
     CDS             complement(1771..2421)
                     /locus_tag="VF995_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343669.1"
                     /translation="MFLRRSKSKGGKRGLPKAGNVRDQVADVTDDLTDSLEAARDAIT
                     KAMRSSSRRGADATRQAARKTTTATKQAGRRGRRAARELSDLVPEPEAIAEATRRATD
                     KLLPALAKQRRKEQRKRKRRLITRGAGLLGLLGAAGMIITGKLRRRGEAARYPYQTEP
                     APGAGMADGRPASATATGPQQSGSTTPASGAEAGAGPAVAHIRDEGAPATSNRRKS"
     gene            complement(2526..3332)
                     /locus_tag="VF995_08665"
     CDS             complement(2526..3332)
                     /locus_tag="VF995_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343670.1"
                     /translation="MDVRLTGVYVADARLLAEGVAQPEDAMAVFVGRAGHGRVAPFTV
                     RRSYTGAGGWYVESMALLAPNGDVAWRGAEQQITLRGSSKIDSLSDDIHGVSVDSADD
                     HELVLLIAGDEVGRVPLAILDEDPPYATASAAAVLDEALKKSTVVWVEVPGGAGGAGT
                     GGRAVPAWYGTLDGRVYILTGGEEQRIPGLAEADRVLLSARSKELQSLVAKVEASARV
                     VPATDPLFARLVPVLLPRRLNLRDGEEAGDRWRKESTLVELTPSGVPADA"
     gene            3463..3942
                     /locus_tag="VF995_08670"
     CDS             3463..3942
                     /locus_tag="VF995_08670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9343671.1"
                     /translation="MVARRSQAGQQVTYQAAKSESGELRRFLAAEWRRADQRLFGATP
                     DWTSQPLVIEAHAGRELIGTAIGEVIAGIARLNDVLVTHERQRGGIGGHLVERFCARA
                     GELGAARCYLRCPATDSHRRFYERHGFVLVARLPRYYHDHDFLEYLRDPIQTPPGRG"
     gene            complement(3943..4668)
                     /locus_tag="VF995_08675"
     CDS             complement(3943..4668)
                     /locus_tag="VF995_08675"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9343672.1"
                     /translation="MHGLRERKKQRTRQAIAAAALRLFIERGFEQVTVAEVAAAAEVA
                     EKTVFNYFPTKEDLFFADAAAMDSGVIIDAIRQRPAGESPLDAFRRLLADKPAFEAEA
                     GQAGPFTPAQLDIHQQAMWVLQRSPALRTHLREQFARLEASIADLLAEETGAGANDVE
                     PKVAAAALVGVIRAVTDRFLAQAAANQDDQDRHGLMAALAEDTTRALDLLERGLSGYA
                     AAAPRPQPAADGTEEEEAGPPSG"
     gene            4783..5154
                     /locus_tag="VF995_08680"
                     /pseudo
     CDS             4783..5154
                     /locus_tag="VF995_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5583..6050
                     /locus_tag="VF995_08685"
     CDS             5583..6050
                     /locus_tag="VF995_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9343673.1"
                     /translation="MVSVQPTDETSTPAGRQTVSRVRTAAAAFPGVRVGGSGPLDVDF
                     GHAVYSRFPLMLAAIAVVTFVLLARAFRSLLLPLKAVLLNLLSVGAIYGALRLVWQDG
                     HGSGLWGIPATGSVDTFIPLMVFAFLYGLSMDYEVFIRRWQAARRLQSEWVLT"
     gene            6090..6701
                     /locus_tag="VF995_08690"
     CDS             6090..6701
                     /locus_tag="VF995_08690"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9343674.1"
                     /translation="MTPTRTAKAKDAILTAADELIRREGFARLTTKQIARAAGISEAS
                     IYYHYADKLDLVQAVLATHLARLLPVTRSLPERAGSGTVAANLEAVATALVSFYQHVI
                     PVAAAAQTDAELLARFRRQLADSGQGPHRGVEVVADYLRAEQRLGRVSAAADPDAAAM
                     LLVGACHQVAFESHLLGTPPPPSADPAALVAALLGLGGLTPQS"
     gene            6727..7182
                     /locus_tag="VF995_08695"
     CDS             6727..7182
                     /locus_tag="VF995_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9343675.1"
                     /translation="MVTIHTHAETTVPADRVVAALTDFSDHRFELFANLDRRYFQLHD
                     SGPTWAEVTEGSPFAGGIWERTRYDWSKPGTVRLDLVDSNAFAPGSSWEYRVHPETHG
                     HTQVSLTVHRVPRTLKARLLATMLTIFGERVFRSDLAKTLQTIATRTTT"
     gene            7438..>7667
                     /locus_tag="VF995_08700"
     CDS             7438..>7667
                     /locus_tag="VF995_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9343676.1"
                     /translation="MGGLEGIGGTPLVRLERLPAEGSAEIWVKLEAANPTGSYKDRMA
                     LAMIDAAERDGRLQPGQLVVEYTGGSTGSSLAL"
BASE COUNT          995 a         2942 c         2614 g         1116 t
ORIGIN      
        1 tggcctatcc cggcccgggc gagcgggtcg cccgcctgga ggagaccctc aagctgctgc
       61 gggccctgct cgccgacgcc gaggccccga cctcgttcga gggccgcttc taccacaccc
      121 acgacgcccc ggtgatcccc ggcccggtgc agcagccctg gccgccgctg tgggtcggcg
      181 ggcggggcga ccgcatgctc ggcgtgaccg cccgcgccgc cgacggctgg aattactgct
      241 ggtcggccac cccggagcag tacggtgagc gtctggaggt cctggccagg gcctgcgccc
      301 gcgcgggacg gccgttcggt gcggtacgcc gcagcctcgg cctctccacc ctggtcggcc
      361 gcgaccccga cgacctggtg gcgcgctggc gccgcctgcg ggcatgggcg cccggcggcg
      421 cgctcgatac ggtcgagctg gccgactggg cggcctcgcg cctggtcggc acccccgatg
      481 aagtcgtcgc ccagttgcgc ggctggcagg cgcgcggtat cgagcaggtg atctgctcct
      541 tcgcgaacgt ccccttcgcc atcttcgagg acgaccagct cgacctggtc gccgagctgg
      601 tcctgcccaa gctgtaggca cctctcagtc agaagcggac ggcgagcagc tcgaagctga
      661 tcgtcaccgg gaccggcgcg tccaggtcca cgccgctggc ttcgatctcg gcgcacagcg
      721 gcccaaggct cgcctgccac gccgcgtcgc cgaccagcca ggtgtcggag aagagccgct
      781 gctccatgag gcgcaggctc tcgccccagg tctcgtccac ctgccaggcg accggcggca
      841 gctcctcggc ctggccgccg aggtcggtga tcgccgcggc gacctcctcg ggggtctgga
      901 cgccgggtcg cttggggtgc ccgccgaggc cttcgaccag cgcccgccag cgccgtcgag
      961 tctcccgcca ggggccgccg ccgtgcgaca cgctgccgcg cttgcccatc agcagcaccc
     1021 cgccaggggc gagcaccctc cgggcttcgg ccaggacctg ccgccacgcg gggatcaggt
     1081 gcagcacgtg gacctcgacg accgcgccca cgccgccgtc gcggaccggc aggcggctga
     1141 cgtcgccccg cagcagcggc gggcgcggga ggccggccgc cgccgcgttg gcgcggtagc
     1201 gctccagcat cgggagcgac aggtccaccc cgaacacggt gcgcccgcgc cgctgcagcg
     1261 gcagggcgat cctgccggtg ccgacgccca gctccaggag gcgcgcgtcc ggcccggccg
     1321 ccgcctcgat gcggtcggtg acctggtcgc tcacctcggc cggcaggcca cgggtgtcgt
     1381 cgtagtagcc ggccgcccgg tcgaagacga tcgagccgcc agcgccctcc acttcccgtt
     1441 ccctcatggc agcgcatatc cagttgtcgg tgttgctagg ctgggaccgc gatggaaccg
     1501 agagaagctt gcctgaaagt tctcgacgac gccgcccccg agccgctcca ctggacggtc
     1561 gtgctcgacc gggcgttgcg cgagcgcctg ctcgacccct tcaccgtgca cgacgtgcgc
     1621 ggggcggtgg tgaagaacct cgcggccctc gccaaggagg gccgcgcggt caaggttggc
     1681 accggcctgt accgagccgc cggcacgcag ccagacggct cggcctaggc cccggccggg
     1741 gtcccgttcc ggtcggccgg actggtcagc tcagctcttg cggcggttcg aggtcgccgg
     1801 ggcgccctcg tcccggatgt gcgccaccgc cgggccggcc ccggcctcgg ccccggacgc
     1861 gggcgtggtg ctgccgctct gctgcggtcc ggtcgcggtc gcgctggcgg gccgcccgtc
     1921 ggccatgccg gcacccggcg cgggctcggt ctggtaggga tacctggccg cctcgccgcg
     1981 gcggcgcagc ttgccggtga tgatcatccc ggccgcgccg agtaggccca aaaggcccgc
     2041 gccgcgcgtg atcaggcgcc gcttgcgctt gcgctgctcc ttgcggcgct gcttggcgag
     2101 tgccgggagc agcttgtcgg tggcccggcg ggtcgcctcg gcgattgcct ccggctcggg
     2161 caccaggtcg ctcagctccc gcgccgcgcg cctgccgcgc cggcccgcct gcttggtggc
     2221 ggtggtggtc ttgcgggccg cctgcctggt cgcgtcggcc ccgcggcggc tgctcgaacg
     2281 catcgccttg gtgatcgcgt ccctggcggc ctccagggaa tcggtcaggt catcggtgac
     2341 atcggcgacc tggtcgcgga cgttgcccgc cttcggcagc ccgcgcttgc cacccttgct
     2401 cttgctcctg cggagaaaca tggtcctcct ccgtcgctga ggcctggatc gcgggttggg
     2461 gtggcagccc ctcgccgccg gcgcttcgac ctactggtgc ctttggtacc cgtcaagcgc
     2521 ccaggtcatg catcggcggg aacgcccgac ggtgtgagct ccaccagggt ggattccttg
     2581 cgccagcggt cgccggcttc ctcgccgtcg cggaggttga ggcggcgggg gagcaggacc
     2641 ggaacaaggc gggcgaacag cgggtcggtg gcgggcacca cccgggcgct ggcctcgacc
     2701 ttggccacca gcgactgcag ctccttggag cgggccgaca gcagcacccg gtcggcctcg
     2761 gcgagccctg ggatgcgctg ctcctcgccg ccggtgagga tgtagacgcg gccgtccagg
     2821 gtgccgtacc aggccggcac cgctcggccg ccggtcccgg cgccgccggc gccgcccggc
     2881 acctcaaccc acaccaccgt gctcttcttc agcgcctcgt cgagcacggc cgccgccgag
     2941 gcggtcgcgt acggcgggtc ctcgtccagg atcgccagcg gcacccggcc gacctcgtcc
     3001 ccggcgatca acaggaccag ctcgtggtcg tcggcggagt cgaccgagac cccgtggatg
     3061 tcgtcggaga ggctgtcgat cttgctggag ccgcgcaggg tgatctgctg ctccgccccg
     3121 cgccaggcca cgtccccgtt cggggcgagc agggccatgg actccacgta ccagccgccc
     3181 gcccccgtgt aggagcgccg gacggtgaag ggcgccaccc tgccgtggcc ggcccgcccc
     3241 acgaacacgg ccatggcgtc ctcgggctgg gccacgccct cggcgagcag cctggcgtcg
     3301 gcgacgtaga cgccggtcag gcggacgtcc atcgggttcc tttcgtcgcg cggttcattg
     3361 cgaagtcgcc gctcctgtca cgacctgcgg cggaatgatc atagacaacc gcaggacttc
     3421 gtggccgggt accatgccag ccactcgaca aggaggcctg acgtggtcgc gcgccgctcc
     3481 caggcaggtc agcaggtcac ctaccaggct gccaagagcg agagcggcga gctgcgccgc
     3541 ttcctcgccg ccgagtggcg gcgggcggac cagcggctct tcggcgcgac gccggactgg
     3601 accagccagc cgctggtgat cgaggcccac gccggccgcg agctgatcgg caccgccatc
     3661 ggcgaggtga tcgccgggat cgcccgcctc aacgacgtgc tggtcaccca cgagcgccag
     3721 cgcggcggca tcggcggtca cctggtcgag cgcttctgcg cccgcgccgg cgagctgggt
     3781 gccgcccgct gctacctgcg ctgccccgcc accgacagcc accgccgctt ctacgagcgc
     3841 cacggcttcg tgctggtcgc ccgcctgccg cgctactacc acgaccacga cttcctcgag
     3901 taccttcgcg acccgatcca aaccccgccg ggccgaggct gatcagcctg aaggaggccc
     3961 ggcctcctcc tcctccgtgc catccgctgc gggctgcggg cgcggggcgg cggccgcata
     4021 accgctgagg ccgcgttcga gcaggtcaag ggcccgcgtg gtgtcctcgg cgagggcggc
     4081 catgaggcca tgccgatcct ggtcgtcctg gttggcggcg gcctgggcga ggaaccggtc
     4141 ggtgaccgcc cggatcaccc cgaccagggc ggcggccgcg accttcggct cgacgtcgtt
     4201 ggcaccggcg ccggtctcct cagcgagcag gtccgcgatg gacgcctcca ggcgggcgaa
     4261 ctgctcccgc aggtgcgtcc gcagcgcggg gcttcgctgc agcacccaca tggcctgctg
     4321 gtggatgtcg agctgggccg gcgtgaaggg gccggcctgg ccggcctcgg cctcgaaggc
     4381 gggcttgtcg gcgagcaggc gccggaaagc gtcgagcggc gactcgcctg cgggacgctg
     4441 gcggatcgcg tcgatgatca cgccgctgtc catcgcggcg gcgtccgcga agaagaggtc
     4501 ctccttggtc gggaagtagt tgaagacggt cttctccgcc acctcggcgg ctgcggccac
     4561 ctcggccacg gtgacctgct cgaagccgcg ctcgatgaag aggcgcaggg cagccgcggc
     4621 gatcgcctgc cgcgttcgct gcttcttgcg ttctcgaagg ccgtgcatgc tccaagcata
     4681 gcgcgattcc tgcggtcagc ataaaaatac tgcagttata aatttctagt gactgtgctt
     4741 acctggaaag gtccgcatcg ccaacacgta tccgcaacgg aagtgaagga ccagtcgtat
     4801 gacacgactc tccacgttcg tcctccggca caagcggctc gtcgtcctgt tctggctgac
     4861 cctggcggtc gccgggatgg cggcgagccc caagctctcc acccgcctct cgcagcagtt
     4921 cagcttgccc ggccagagag ctacgaggcc aaccaggcga tcctgcgcac ctacggcaac
     4981 ggcggcaaca ccctgccgct ggtcgggatc gtctccgcgc cctcgggcac caccctcgac
     5041 agtcccgccg cgcggcaggt gctcgcccgc gccttcgccc aggccgccca gacccaggcc
     5101 gtcgtccggc cgggcatcgc cccagctggg cgggtcgtct cctacgccag caccggcgac
     5161 cagcgcctgc tcagcgccga ccaccgcagc gcctacgggc tggtctaccc gccctcccgc
     5221 gccgcggaga tgggcagccc cgacctggtc acaccagtgg gcgccgcgct gcgccagggc
     5281 ctcccggcgg gctggatcgt ccaggtcacc gggctggacg cgctgcagtc cggcggcgac
     5341 gccaacggcc ccggcgtgct cgccgagacc ctgctcggcg ccgtcggcgc gctggcggtg
     5401 ctggcgttcg tgttcggctc gctgctggcg ctggtgccgc tgctgatcgc ggcggtgtcg
     5461 atcctggcga ccttcctgat catcctcggc ctcaccgagc tcaccgaggt gagcgtgatc
     5521 gtgcagtacc tggtcgcgct gatcggcctc ggcgtggcgc ttggcggcac ggcggcaccg
     5581 cgctggtcag cgtccagcca accgacgaga ccagcacccc ggcgggcagg cagaccgtca
     5641 gcagggtccg cacggcagcc gccgcctttc ccggcgtgcg ggtgggcgga tccggcccgc
     5701 tggacgtcga cttcggccac gccgtctaca gccgcttccc gctgatgctc gccgccatcg
     5761 cggtggtgac cttcgtgctg ctggcccgcg ccttccgctc gctgctgctg ccgctcaagg
     5821 cggtgctgct caacctgctc agcgtcgggg cgatctacgg cgcgctcagg ctggtctggc
     5881 aggacggcca cggctcgggc ctgtggggca tccccgccac cggctcggtg gacaccttca
     5941 tcccgctgat ggtgttcgcg ttcctgtacg gcctgagcat ggactacgag gtgttcatcc
     6001 gacgctggca ggcggctagg cgcttgcaat ctgagtgggt actcacttag tatagtgagt
     6061 acccactcag ttacgggagg cgccatgcca tgaccccaac ccgcaccgcc aaggccaagg
     6121 acgcgatcct caccgccgcc gacgagctga tccggcgtga gggcttcgcc cgcctgacca
     6181 ccaagcagat cgcccgtgcc gccgggatct cggaggcgag catctactac cactacgccg
     6241 acaagctcga cctggtccaa gcggtgctcg ccacccacct ggcccgcctg ctcccggtga
     6301 cccggtcgct gccggagcgt gccggcagcg gcaccgtcgc cgccaacctc gaagccgtgg
     6361 ctaccgcgct ggtcagcttc taccagcacg tcatcccggt cgccgccgcc gcccagaccg
     6421 atgccgagct gctcgcccgc ttccgccgcc agctcgccga cagcggccag ggcccccacc
     6481 gcggcgtcga ggtcgtcgcc gactacctgc gcgccgagca gcgcctcggc cgcgtcagtg
     6541 ccgccgccga tcccgatgcc gccgccatgc tgctcgtggg cgcctgccac caggtcgcct
     6601 tcgagtccca cctgctcggc accccgcccc cgccgagcgc cgatcccgcc gcactcgtcg
     6661 ccgccctgct cggcctcggc ggcctcaccc ctcaatcctg actcgatcct gaacggaggg
     6721 atccccatgg tcactatcca cacccacgcc gagaccaccg tccccgccga ccgcgtcgtc
     6781 gccgccctca ccgacttctc cgaccaccgc ttcgagctgt tcgccaacct cgaccgccgc
     6841 tacttccagc tccacgactc cggccccacc tgggccgagg tcaccgaggg ctccccgttc
     6901 gccggcggca tctgggagcg cacccgctac gactggtcga agcccggcac ggtccgcctc
     6961 gacctggtcg actccaacgc cttcgccccc ggcagctcct gggagtaccg cgtccacccc
     7021 gagacccacg gccacaccca ggtcagcctg accgtccacc gcgtcccccg caccctcaag
     7081 gctcgcctgc tggcaaccat gctcaccatc ttcggcgagc gcgtcttccg cagcgacctc
     7141 gccaagaccc tccagaccat cgccaccagg acaaccacct gacagcgaca gcgagcgcgc
     7201 cggtcagagg ttcccccctg accggcttcc tcgtcccggc ataacggata atcggtattc
     7261 acctcgcggg agagccgata gggttggtca gtgaggcgcc aaccaggacg ggcggctcgc
     7321 cgtgcgtgga tgttctattt cgataccgtc gcgctggccg agttcctcgc caacgcagca
     7381 cggccagggc cataggcagg ggccacaggc caggccgacc gagcgatcgg agggagcatg
     7441 ggcggactcg aaggcatcgg cggcaccccc ctcgtgcggc tggagcggct gcccgccgag
     7501 ggcagcgccg agatctgggt gaagctcgag gcggccaacc cgaccggctc ctacaaggac
     7561 cggatggccc tggccatgat tgacgctgcc gagcgcgacg ggcgcctcca gccggggcag
     7621 ctggtggtcg agtacaccgg cggcagcacc gggtcgtcgc tcgcgct
//
LOCUS       DASWDC010000269         8914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1125528, whole genome shotgun sequence.
ACCESSION   DASWDC010000269 DASWDC010000000
VERSION     DASWDC010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125528"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2509)
                     /locus_tag="VF995_08705"
     CDS             complement(<1..2509)
                     /locus_tag="VF995_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9343677.1"
                     /translation="MPNREGAVGAGARLAATGRSKPFVGRAQDLAALRSVLRAAVEGH
                     GSLVLLAGEPGIGKTCLAERLAIEAEAAPGGAAILWGSAWEGGAPPFWPWVQILRQLL
                     RQLPATDEQFDALLLEGDPGMAEVARILPELAGSAPRPAELAVAPAEQARFRLFGAVA
                     ALLARASAVRPLLLVLDDLHWADMPSLRLLRFLAQELEGVRVLLLGAYRDVEAPAARR
                     IEEVAASLGDACARLALRGLGLDEVGQLIGLTTGAEPSPEEAAGALQHAGGNPLFVRE
                     VARLLAAGVPGRAVPAGVRPVLARRLEQLRPDAAELLATASVLGTELRPELLGTLVGA
                     RAERIVALLGEAADARLVELVAGPPARWRFAHELVREVLYERLPLARRQALHRQAGEA
                     IEARLPTDLELHLGELAGHFLHAGSAGAAKAAGYAERAGRHALDMLAWEDAAGHFQRA
                     LEALDLMPDRADRAGRLPVELTRRLDLDLELELAGVRMILGEVAAARAGYERAAVLAR
                     RLGSADHLARAALGLGAEEIIFTVDESQVSLLEEAIGQLGPGGPLRARALARLAKALE
                     YGPDIDRRIALAGEALAIARQVGDPATLAAVLVDHHRATWGGRPPADQLDTATEIVAL
                     AEAGGDRFLALQGRLYRSLDLLELGDIVAMRAEVEAFSRGAEASRQPRLQWLSCQLVG
                     TLALIDGRFEEVRRRNEETLVIAQRAGDPYGVMSYLGLGGVLSAVIGDFSAVVEPLRQ
                     AARGLPAFMVQAAVVVGLLNTGQPAQAREEFERLAAGDFAGLFPAASYVMNAAVASMA
                     AYGLGDAARARVLYERLAPYAGQVIRASRLAGGCFWAV"
     gene            complement(2514..4118)
                     /locus_tag="VF995_08710"
     CDS             complement(2514..4118)
                     /locus_tag="VF995_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9343678.1"
                     /translation="MMDYPLTLRGVYERARALFPDRQLVSRRAGGEIARIGYADWAER
                     VARLATALRELGVGSGDRVGTFCWNHDRHLEVYFAAALSGASYHTLNIRLGPDQLAYI
                     ANHARDKVLIADAALVPALAPVLPETKTVEHLVVIGDDEGAPPHELPFGSVHDYETLL
                     ARAAPMTDWPELDERQTAGICYTSATTGNPKGVAYSHRALFLHSLMIGLADTVAVSER
                     DVVMPIVPMFHVNAWGLPFAATWMGAGQVFGGPQPTPADYLKLIREQGVTISAGVPTV
                     WLGILQVLERDGGDLGPLRRIMCGGSAAPVSMIRTYEQRYGVEFIHAYGMTEASPVTH
                     FSRLKSSLLGLPEDGRLGYKAKQGLLVPGLEQRIAGEDGEPVPGDGKSMGEVWLRGPW
                     VADEYLDDPRSADTFVGGWYRSGDVATLDGDGYMQLVDRTKDLVKSGGEWISSVELEN
                     ALMGHPEVAEAAVIAVPDPKWDERPLAVVVRKPGSSLGGEELIASIAGQFPKWWLPDR
                     VEFTDELPKTATGKFSKRILRERYAG"
     gene            4393..6492
                     /locus_tag="VF995_08715"
     CDS             4393..6492
                     /locus_tag="VF995_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX9343679.1"
                     /translation="MGGAVGDVRVGVDVGGTFTDLVALVDGQVVSAKVPSTPRDQAQG
                     VMHAIGLAELAAERVAVIAHGSTVATNALLERGGARTALVTTEGFRDVIEIGRQHRPA
                     LYDLTRDRPPPLVPRELRFTVRERMGPGGVLVPLDEASLAEAVEACRAAGVDAVAVCL
                     LFGFLHPAHELRVGAALRDALPGVHVSLSCQVLPEFREFERCATTVADAYLTPRLGAY
                     LRSLSGQLRQRALPGPMVMQSSGGVAEVEEAAAHAAACVLSGPAGGVVGAAWIAGLSG
                     HRDLLTFDMGGTSTDVALVRGGVVATTTESVVGGVPIRLPVVDIHSVSAGGGSIAWVD
                     DGGSLRVGPRSAGADPGPAAYGRGGSDSTVTDANLALGYLADGAVLGGEVRLDRARAE
                     AALARLGERLGLDPHRAAVGVVRVANAEMNRALRVISVQRGIDPREHTLVAFGGAGGM
                     HACALAEELGITRVLLPGVAGVLSALGLAIADLRRDYLRPFLGALDETDPEALEAAWK
                     ELEARASADAGAGGSGSMVPRPEAPQADPTAAAGPPDGSITEASALQLTRQADLRYHK
                     QSFELTITAPSPDPGELAVRFHEAHERRYGYRMLEEPVELVNLRLVAVTPVEKPSLST
                     NTFIQSRSPTRRRASFDDRQQEVETLDRASLGVGVAVGGPAIVASSDATCVVRPGWHG
                     VVDEVGSLLLERTREGV"
     gene            6492..8030
                     /locus_tag="VF995_08720"
     CDS             6492..8030
                     /locus_tag="VF995_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX9343680.1"
                     /translation="MEGGPLDPITLSVLSSALAGIGEEMGTALVRSAYSANIKERRDC
                     SAALFDAAGRMVAQAEHIPVHLGAMPEAVAAVIACDPAPGDVHVLNDPYSGGNHLPDV
                     TMVAPLDVDGERLAYAVTRAHHSDIGGMQPGSMPAGSRDIFSEGLVIPPVRLVADGAY
                     VPDVLKLLLANTRTPAIRRGDFRAQIAANRLAQERLGELVGRWGRELVLASFDGVIAA
                     TERRTREAIRRLPDGEYTAASELEGDGVLDDDIPIRVTVQIRGDELRVDFSGTAGAVP
                     GNVNCPLAVTRSACIFALRVLVADDLPTNAGTWAPLDIQAEPGSLVNARRPSAVVAGN
                     VETSQRLADTVLAALGQATDLPAAGQGTMNNVNIGGRGWTYYETIGGGQGASASGPGP
                     SGVHVGMSNTLNTPIEAFELEYPMRVERFELAAGSGGAGRHRGGDGIIRAIRVLEPAT
                     LSLLTDRRRHRPPGSHGGEPGQCGRNELNGEPLPPKVNVALSPGDVVTVTTPGGGGWG
                     RAEP"
     gene            complement(8034..8417)
                     /locus_tag="VF995_08725"
     CDS             complement(8034..8417)
                     /locus_tag="VF995_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9343681.1"
                     /translation="MPHYSRLYRVVVDVPEADHDEELAFWQGAIGKPLDQSKRHPEYH
                     GTDLADDAWGILVQKLGEGTNRVHLDIHTDDLEAEVARLKGLGAEEVQRINRWCVMRD
                     PAGMVFCVIPDPPGTLNDRNARRWD"
     gene            complement(8488..>8914)
                     /locus_tag="VF995_08730"
     CDS             complement(8488..>8914)
                     /locus_tag="VF995_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9343682.1"
                     /translation="PDDLDELLCRRRKSHFFLAHPGAIAAFGRECNRRGIYPPTVEVE
                     GRRVHAWRGVPLLPCDKLPVSPSRTTAILVMRTGQEDQGVVGLHQTGIPDEYQPSLSV
                     RFMGVDDQAVMSYLVSAYYAAAVLVPDALGILENVELGR"
BASE COUNT         1194 a         3338 c         3264 g         1118 t
ORIGIN      
        1 tgaccgccca gaagcatccc ccggccagcc ggctggcgcg gatcacctgg ccggcgtacg
       61 gcgccaggcg ctcgtacagg acgcgggcgc gggcggcgtc gccgaggccg taggcggcca
      121 tgctggcgac ggcggcgttc atgacgtagc tggcggcggg gaacaggccg gcgaagtcac
      181 cggcggccag gcgttcgaac tcctcgcggg cctgcgccgg ctggccggtg ttgagcaggc
      241 cgaccaccac agcagcctgg accatgaagg ccggcagccc ccgcgcggcc tgccgaagcg
      301 gctccaccac cgcgctgaaa tcgccgatga cggcgctcag cacgccacca agacccaggt
      361 acgacatcac cccgtagggg tcgccggcgc gctgggcgat caccagcgtc tcctcgttgc
      421 gccgccgcac ctcctcgaaa cggccgtcga tcagggcgag ggtgccgacc agctggcagc
      481 tcagccattg cagccgcggc tgccgcgacg cctcggcgcc acgcgagaag gcctcgacct
      541 ccgcgcgcat ggccacgatg tcacccagct ccagcaggtc gaggctgcgg tagaggcgac
      601 cctggagggc gaggaaccgg tcgccgcccg cctcggcgag cgccacgatc tcagtggcgg
      661 tgtcgagctg gtcagcgggc ggcctgccac cccaggtggc ccggtggtgg tcgaccagga
      721 ccgccgccag ggtggccggg tcgccgacct gccgggcgat ggcgagcgcc tcgccggcaa
      781 gggcgatccg ccggtcgatg tctggcccgt actcgagcgc cttcgccagc cgggccagcg
      841 cccgcgcccg cagcgggccg ccgggcccga gctggccgat cgcctcctcc agcaggctga
      901 cctgcgactc gtcgacggtg aagatgatct cctccgcgcc cagcccgagc gcggcacggg
      961 cgagatggtc ggcgctgccg agccgccggg ccagcacggc ggcccgctcg tagccagcgc
     1021 gggcggcggc gacctccccg aggatcatcc gcaccccggc cagctccagc tccaggtcca
     1081 ggtccaggcg ccgggtcagc tccaccggca gccggccggc ccggtcggcc cggtcgggca
     1141 tgaggtcgag ggcctccagg gcgcgctgga aatgaccggc tgcgtcctcc caggcgagca
     1201 tgtcgagggc atggcggccg gcgcgttcgg cgtagccggc cgccttggcg gcacccgccg
     1261 atccggcgtg caggaaatgg ccggccaact cgccgagatg gagctccagg tcggtgggca
     1321 agcgcgcctc gatcgcctcc cccgcctggc ggtgcagcgc ctgccggcgc gccagcggca
     1381 ggcgctcgta gaggacttcc cggaccagct cgtgggcgaa gcgccagcgg gcgggcgggc
     1441 cggccaccag ctccaccagc cgggcgtccg ccgcttcgcc gagcagcgcc acgatccgct
     1501 cggcgcgggc gccgacaagg gtccccagca gctccgggcg cagctcggta ccgagcaccg
     1561 aggcggtcgc cagcagctcg gcggcgtcgg gccggagctg ctccagccgc cgggcgagca
     1621 ctggccgcac ccccgcggga accgcgcggc cagggacccc ggcggccagc agacgagcga
     1681 cctcgcggac gaacagcggg ttgccgccgg cgtgctggag cgcaccggcg gcctcctccg
     1741 gggacggctc ggcgccggtg gtgaggccga tgagctggcc gacctcgtcc aggccgaggc
     1801 cgcgcagcgc cagccgggcg caggcgtccc ccagcgaggc ggcgacctcc tcgatacgcc
     1861 gggccgcggg cgcctcgacg tcgcggtagg cgccgagcag cagcacccgg acgccctcca
     1921 gctcctgagc gaggaagcgc agcagccgta gcgacggcat gtccgcccag tgcaggtcgt
     1981 cgaggaccag cagcagcggc cgcaccgccg acgccctggc cagcagggcc gccaccgcgc
     2041 cgaacaggcg gaagcgggcc tgctccgccg gcgccaccgc cagctcggcc ggcctgggcg
     2101 cggatccggc cagttcgggc aggatccgcg ccacctcggc catccccgga tcgccctcca
     2161 gcaggagcgc gtcgaactgc tcgtcggtgg cggggagctg gcgcagcagc tgccgcagga
     2221 tctggaccca cggccagaac gggggtgcgc cgccttccca ggcgctgccc cacaggatgg
     2281 ccgcaccgcc cggcgctgcc tcggcctcga tggccaggcg ctcggccagg caggtcttgc
     2341 cgatcccggg ctcgccggcc agcagtacca gcgacccgtg gccctcgacc gccgcccgca
     2401 gcaccgaacg gagcgcggcg aggtcctggg cgcggcccac gaacggcttg gacctgccgg
     2461 tcgcggcgag ccgcgcgccg gccccgacgg ccccctcccg gtttggcatc cgctcagccc
     2521 gcgtagcgct cgcgcaggat gcgcttggag aacttgccag tagcggtctt gggcagctcg
     2581 tcggtgaact ccacccggtc cgggagccac cacttcggga actggccggc gatcgaggcg
     2641 atcagctcct cgccgccaag tgacgacccg ggcttgcgga ccaccaccgc cagcggccgc
     2701 tcgtcccact tcgggtcggg cacggcgatc accgcggcct ccgcgacctc gggatggccc
     2761 atcagggcgt tctccagctc gaccgaggag atccactcgc cgccggattt caccagatcc
     2821 ttggtgcggt cgaccagctg catgtagccg tcgccgtcga gggtggcgac gtcgcccgat
     2881 cggtaccagc cgccgacgaa ggtgtcggcg gagcgcgggt cgtccaggta ctcgtcggcg
     2941 acccatgggc cgcgcagcca cacctcgccc atggacttgc cgtcgccggg caccggctcg
     3001 ccgtcctcgc cggcgatgcg ctgctccagt ccggggacca gcaggccctg cttggctttg
     3061 tagccgaggc gcccgtcctc ggggaggccc agcagcgagg acttcagccg cgagaagtgg
     3121 gtgaccggcg acgcctcggt catgccatac gcgtggatga actcgacgcc gtagcgctgc
     3181 tcgtaggtgc ggatcatcga caccggcgcc gccgacccgc cgcacatgat ccgccgcagc
     3241 gggccgaggt cgccgccgtc ccgctccagc acctggagga tcccgagcca gacggtcggg
     3301 acgccggccg agatggtcac gccctgctcg cggatcagct tgaggtagtc ggccggggtt
     3361 ggctgcgggc cgccgaacac ctggccggcg cccatccagg tggccgcgaa cgggaggccc
     3421 caggcgttga cgtggaacat cggcacgatc ggcatgacca cgtcgcgctc ggagacggcg
     3481 acggtgtcgg ccagcccgat catcagcgag tgcaggaaca gcgcccggtg gctgtaggcg
     3541 acgcccttgg ggttgccggt ggtcgccgag gtgtagcaga tcccggcggt ctgccgctcg
     3601 tccagctcgg gccagtcggt catgggggcg gcccgcgcca gcagcgtctc gtagtcgtgc
     3661 accgacccga acgggagctc gtgcggcggc gcgccttcat cgtcgccgat gacgaccagg
     3721 tgctcgaccg tcttcgtctc gggcaggacg ggcgcgagcg ccgggacgag cgcggcgtcg
     3781 gcgatcagga ccttgtcgcg ggcgtggttg gcgatgtagg cgagctggtc ggggccgagg
     3841 cggatgttga gggtgtggta gctggcaccc gacagcgccg ccgcgaagta gacctccagg
     3901 tgccggtcgt ggttccagca gaaggtcccg acccggtcgc cgctgccgac gccgagctcc
     3961 cggagcgcgg tggcgaggcg ggcgacccgc tcggcccagt cggcgtagcc gatgcgggcg
     4021 atctcacccc ccgcgcgcct gctgacgagc tgtctgtctg ggaacagcgc ccgcgcgcgc
     4081 tcgtacacgc ccctgagggt gagcggatag tccatcatcg cgcagacctc cctgctccag
     4141 ctggttcggc gcgccaaatc ccttccgccg agatcatcaa cgatcctatg ggcgccggcc
     4201 gcgaaggtcg agaggggacc gctcggctga cgctggggac ggccgccgag ggaaaagggg
     4261 tcagcgggat tccatcgccc acctcttgac gaatcgggca atggagaacg ttatatgttc
     4321 tcatcgacat gataagtcga ggaggccgag cgcctcgccg ccgccagcga ctggcgcgag
     4381 gagggctgat ccgtgggggg cgccgtgggg gacgtcaggg tcggcgtcga cgtgggcggc
     4441 accttcaccg acctggtcgc cctggtcgac ggccaggtgg tcagcgccaa ggtcccctcc
     4501 accccccgcg accaggcaca gggggtgatg cacgcgatcg ggctcgccga gctggcggct
     4561 gagcgggtcg ccgtgatcgc ccacggcagc acggtggcga ccaacgccct gctggagcgc
     4621 ggcggcgccc gcaccgcgct ggtcaccacc gagggcttcc gggacgtgat cgagatcggc
     4681 cggcagcacc gcccggccct ctatgacctg acccgcgacc ggccaccgcc gctggtcccc
     4741 cgcgagctgc gcttcaccgt ccgcgagcgg atgggccccg gcggcgtgct cgtccccctg
     4801 gacgaggcct ccctggccga ggcggtcgag gcctgccgcg ctgccggggt ggacgcggtc
     4861 gcggtctgcc tgctgttcgg gttcctgcac cccgcgcatg agcttcgggt cggcgcggcg
     4921 ctgcgggacg ccctcccggg cgtgcacgtc tcgctgtcgt gccaggtgct gcccgagttc
     4981 cgcgagttcg agcgttgcgc caccacggtc gccgacgcct acctgacgcc ccggctcggc
     5041 gcctacctgc gcagcctctc ggggcagctg cggcagcggg cgctccccgg cccgatggtg
     5101 atgcagtcat ccggcggcgt ggccgaggtc gaggaggccg ccgcccacgc ggccgcctgc
     5161 gtgctgtcgg gcccggcggg cggagtggtc ggggcggcct ggatcgccgg gctgagcggc
     5221 caccgcgacc tgctcacctt cgacatgggc gggacctcca ccgacgtggc gctggtccgg
     5281 ggcggtgtgg tggccaccac caccgagtcg gtggtcggcg gggtcccgat ccggctgccg
     5341 gtggtcgaca tccacagcgt gagcgcgggc ggcggctcga tcgcctgggt ggacgacggc
     5401 ggcagcctgc gggtcggccc gcgctcggcc ggcgccgacc cgggcccggc cgcctacggc
     5461 cgcggcggca gcgactcgac cgtgaccgac gccaaccttg ccctcggcta cctcgccgac
     5521 ggcgccgtgc tcggcggcga ggtgcggctg gaccgggccc gcgccgaggc cgcgctcgcc
     5581 cgcctcggcg aacgcctcgg cctggacccg caccgggccg cggtgggcgt ggtccgggtg
     5641 gccaacgcgg agatgaaccg ggcgctgcgg gtgatcagcg tgcagcgcgg gatcgacccg
     5701 cgcgagcaca ccctggtcgc cttcggcggc gccggcggga tgcacgcctg cgcgctggcg
     5761 gaggagctgg ggatcacccg cgtgctgctg cccggggtcg ccggggtgct cagcgccctc
     5821 ggcctggcaa tcgccgacct gcgccgcgac tacctgcgcc ccttcctcgg cgccctcgac
     5881 gagaccgacc ccgaggcgct ggaggccgct tggaaggagc ttgaagcccg cgccagcgcc
     5941 gacgcgggcg cggggggttc ggggagcatg gtcccgcggc cggaagcacc gcaggccgat
     6001 cccacggctg ccgcggggcc ccccgatggg tcgatcacgg aggcgtccgc cctccagctg
     6061 acacggcagg cggacctgcg ctaccacaag cagtcgttcg agctgaccat taccgcgccc
     6121 agccctgacc ccggcgagct ggccgtccgg ttccacgagg cgcacgagcg ccgctacggc
     6181 taccgcatgc ttgaggagcc cgtcgagctg gtcaacctgc gcctggtcgc cgtgacgcct
     6241 gtggagaaac cctcactctc aacgaatacc tttatccagt cgagatcccc gacccggcgg
     6301 cgggccagct tcgacgaccg ccagcaggag gtcgagaccc tggaccgcgc cagcctcggg
     6361 gtgggcgtgg cggtgggcgg gccagcgatc gtggcctcct cggacgcgac ctgcgtggtc
     6421 cgccccggct ggcacggcgt ggtggacgag gtcgggtcgc tgctgctgga acgcacccgg
     6481 gagggggtct gatggagggc ggcccgctcg acccgatcac cctctcggtg ctcagcagcg
     6541 cgctggccgg gatcggcgaa gagatgggca ccgcgctggt ccgcagcgcc tactcggcca
     6601 acatcaagga gcggcgcgac tgctcggcgg cgctgttcga cgccgccggg cggatggtgg
     6661 cgcaggccga gcacatcccc gtccacctcg gcgccatgcc cgaggcggtc gcggcggtga
     6721 tcgcctgcga ccccgccccc ggcgacgtcc acgtcctcaa cgatccgtat tcgggcggca
     6781 accacctgcc cgacgtcacc atggtcgccc cgctggacgt ggacggcgag cggctcgcct
     6841 acgccgtcac ccgcgcccac cactccgaca tcggcgggat gcaacccggc tcgatgccgg
     6901 cgggctcacg cgacatcttc tccgaggggc tggtgatccc gccggtccgc ctggtcgccg
     6961 acggcgcgta cgtgcccgac gtgctcaagc tgctgctcgc caacacccgc acccccgcga
     7021 tccggcgggg ggacttccgc gcccagatcg ccgccaaccg cctcgcccag gagcgcctcg
     7081 gtgagctggt cgggcgctgg ggccgcgagc tggtgctggc atccttcgac ggggtgatcg
     7141 ccgcgaccga gcgccgcacc cgcgaggcga tccggcggct ccccgacggc gagtacaccg
     7201 ccgccagcga gctggagggc gacggcgtgc tcgacgatga catcccgatc cgcgtcaccg
     7261 tccagatccg gggcgacgag ctgcgggtcg acttcagcgg caccgccggg gccgtgcccg
     7321 gcaacgtcaa ctgcccgctg gcggtcaccc gctcggcctg catcttcgcg ctgcgggtgc
     7381 tggtcgccga cgacctgccg accaacgcgg gcacctgggc gccgctcgac atccaggcgg
     7441 agcccggcag cctggtcaac gcgcggcggc cctcggcggt ggtcgccggc aacgtcgaga
     7501 ccagccagcg cctcgccgac accgtgctcg ccgccctcgg ccaggcgacc gacctgccgg
     7561 cggcgggcca gggcacgatg aacaacgtca acatcggcgg gcgcggctgg acctactacg
     7621 agacgatcgg cggcggccag ggcgcgagcg ccagcggccc aggcccctcc ggcgtccacg
     7681 tcggcatgag caacaccctc aacaccccga tcgaagcctt cgagctggag tacccgatgc
     7741 gggtcgaacg cttcgagctg gccgcgggca gcggcggcgc gggccgccac cgcggcggcg
     7801 acggcatcat ccgcgcgatc cgggtgctgg agccggccac cctctcgctg ctcaccgacc
     7861 gccgccgcca ccgccccccc ggcagccacg gcggcgagcc gggccagtgc ggccgcaacg
     7921 agctgaacgg cgaaccgctg ccgccgaagg tcaacgtcgc cctctccccc ggcgacgtcg
     7981 tgaccgtcac caccccgggc ggcggcggct ggggccgggc cgagccctga cggtcagtcc
     8041 cagcgccgcg cgttgcggtc gttcagcgtg ccgggcgggt ccgggatgac acagaagacc
     8101 atgccggcgg ggtcgcgcat gacgcaccac cggttgatcc gctggacctc ctcggcgccc
     8161 agtcctttga ggcgtgcgac ctcggcctcc aggtcgtcgg tgtggatgtc caggtggacc
     8221 cggttcgtgc cctcccccag tttctggacc aggatccccc aggcgtcatc cgcgagatcg
     8281 gttccgtggt attcggggtg gcgcttcgac tggtccagcg gcttgccgat cgcgccctgc
     8341 cagaacgcca gctcctcatc gtggtcggcc tccggcacgt cgaccacgac ccggtacagc
     8401 ctgctgtagt gcggcatgtc ctcacctcca caggcatctt gccgcagagg tgccacactc
     8461 gcgccagcgc cagcgccagc gccagcgtca gcggccgagc tcgacgttct ccaggatgcc
     8521 gagggcgtcg gggaccagca cggcggccgc gtagtaggcg ctgaccaggt aggacatgac
     8581 cgcctggtcg tcgacgccca tgaaccggac cgacaggctc ggctggtact cgtcgggaat
     8641 gccggtctgg tgcaggccga ccacgccctg gtcctcctgg ccggtccgca tgaccaggat
     8701 cgcggtggtg cggctggggc tgaccggcag cttgtcgcag gggagcagcg ggacgccccg
     8761 ccaggcgtgg acccggcggc cctccacctc aaccgtcggc gggtagatcc cccggcggtt
     8821 gcattcgcgg ccgaacgcgg cgatggcgcc cggatgggcg aggaagaaat gcgacttgcg
     8881 ccgccggcag agcagctcgt cgaggtcgtc gggg
//
LOCUS       DASWDC010000270         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_76193, whole genome shotgun sequence.
ACCESSION   DASWDC010000270 DASWDC010000000
VERSION     DASWDC010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76193"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VF995_08735"
     CDS             <1..306
                     /locus_tag="VF995_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX9343683.1"
                     /translation="SYQPGVVAEGRVAGGDEDSDEDPLLDQAMEIVVRSGLGSTSMLQ
                     SKMKVGFSRARRIMDQLEERGIVGPSEGSKPRDVLMTVEELEQLRGREAGYGEIVGD"
     gene            435..1223
                     /locus_tag="VF995_08740"
     CDS             435..1223
                     /locus_tag="VF995_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX9343684.1"
                     /translation="MSLKGVSRLSTIGETLRSAREEQGKSIAEAATATRIRSSYIEAL
                     EQERFDELGGSVYAKGFLRSYASFLGLDPEPLLETYHASQPGDRPAFERPPKILGTMQ
                     ARRRSPSWVTIGIVGAAVVLGVGVYSLVRPTAGPAQTAPAPIAAPATTIAPTEAQAVT
                     TTTLPPIKGVNLRLHYRAASWTKVTADGKVEFQGILGTTATTKTFTAKHTMDLVLGAA
                     GAVDITVNGKPFQLPANGGQVVQHHFTAAAGATPLAAGHGGTTG"
     gene            complement(1275..2102)
                     /locus_tag="VF995_08745"
     CDS             complement(1275..2102)
                     /locus_tag="VF995_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9343685.1"
                     /translation="MDPAAVALGDANVAAVAAVLADRGRWRVLAALGDGRALPASMLA
                     AEAGVAASTASEHLARLVGAGLLTVERHGRYRYFRLAGPQVADLLEALARVAPPVPVR
                     SLREGTRAQALRFARTCYDHLAGRVGVAMMDAMLRRGFLEGGGENEVENEVGTEAASG
                     SGAAGLAPARPWGDGLAVPGRAIRYGLTEPGAAFLGELGVDLAALPPRRPAVRYCIDW
                     SEQRPHLAGAVGAALTSRLLALDWLRPAERSRAVHLTDQGRAGLTSVFGIDAERAAS"
BASE COUNT          350 a          870 c          790 g          279 t
ORIGIN      
        1 agctaccaac caggcgtggt cgccgagggc cgcgtcgccg ggggggacga ggactccgac
       61 gaggacccgc tgctcgacca ggccatggag atcgtggtcc gctccgggct cggctccacc
      121 tcgatgctgc agtcgaagat gaaggtcgga ttctcccggg cccgccgcat catggaccag
      181 ctggaggaac gcggcatcgt cggcccctcg gagggctcca agccccgcga cgtgctgatg
      241 accgtcgagg agctggaaca gctccgcggc cgtgaggccg gctacggcga gatcgtggga
      301 gactagcgat cgaaataaag gttcccgcga cgcatgccga atccattgcg gggtaaccac
      361 ctgatagcta gaattcgcac tcgtcaaatc tgaccgaatc tgcggtttgc ccagtcgtcg
      421 tttgggctgg ccgcatgtcg ttgaaagggg tgtcccggct gagcaccatc ggtgagacgc
      481 tccgctcggc tcgggaggag caggggaagt cgatcgctga ggcagcgacc gcgacccgta
      541 tccgctcgag ctacatcgaa gccctggagc aggagcggtt cgacgagctg ggtgggagcg
      601 tgtacgccaa gggcttcctg cgctcctatg ccagcttcct cggccttgac ccggagccgc
      661 tgctggagac ctaccacgcc agccagcccg gcgaccggcc ggcgttcgag cggcccccga
      721 agatcctcgg caccatgcag gcccgccgcc gcagcccgag ctgggtcacc atcgggatcg
      781 tcggcgccgc cgtggtcctg ggcgtcgggg tgtactcgct ggtccggccc accgcgggcc
      841 ccgcccagac ggcaccggcg ccgatcgccg cgccagccac cacgatcgcg ccgacggagg
      901 cgcaggcggt caccacgacg accctcccgc cgatcaaggg ggtcaacctg cggctgcact
      961 accgcgccgc ctcctggacc aaggtcaccg ccgacggcaa ggtcgagttc cagggaattc
     1021 tcggcaccac ggcgaccacc aagaccttca ccgccaagca caccatggac ctggtcctgg
     1081 gcgccgccgg cgcggtcgac atcaccgtca acggcaagcc gttccagctc ccagccaacg
     1141 ggggccaggt cgtccagcac cacttcaccg ccgccgcggg cgccaccccg ctcgccgccg
     1201 ggcatggcgg caccaccggc taggctaggc taggacgcgg acgatcgtac cgcgcgtggc
     1261 cacggcccgg ccgctcagct ggcggcgcgt tcggcgtcga tgccgaacac cgaggtcaag
     1321 cccgcgcggc cctggtcggt gagatggacc gcgcggctcc gctccgccgg tcgcagccag
     1381 tccagcgcca gcaggcggct ggtgagggcg gcgccgaccg cgccggccag gtgcgggcgc
     1441 tgctcgctcc agtcgatgca gtagcgcacc gccgggcggc gtggcggcag cgcggcgagg
     1501 tcgacgccga gctcgccgag gaacgccgct cccggctcgg tcagcccgta gcggatcgcc
     1561 ctgcctggta cggccaggcc gtcaccccag ggccgagctg gggccagccc ggcggcgccc
     1621 gagcccgagg cggcctccgt cccgacctcg ttctcgacct cgttctcacc gccaccctcc
     1681 aggaagccac gccgcagcat cgcgtccatc atggcgacgc cgacccggcc ggcgagatgg
     1741 tcgtagcagg tccgcgcgaa ccgcagcgcc tgcgcccggg tcccctcccg cagcgaccgc
     1801 accggcaccg gcggagccac gcgcgccaat gcctccagca ggtcggcgac ctgcgggccg
     1861 gccaggcgga agtagcggta gcggccgtgg cgctccaccg tcagcagccc ggcgccgacc
     1921 agccgggcaa gatgctcgct ggccgtggac gccgccacgc ccgcctcggc ggccagcatg
     1981 ctggcgggaa gcgcccggcc gtcgccgagc gccgccagca cccgccagcg cccgcgatcg
     2041 gccagcaccg cggcgacggc cgcgacattg gcatctccaa gagcaacagc ggcagggtcc
     2101 atgcccgcac cgtaccaccg caacacttcg tcagagaccg aactgtggca gcgacagtgg
     2161 gtcgtggacg cctgcgccct cgccgccgcc gcgttcgcgc cagcgccctc ctgcccgcga
     2221 ccacggaccc ctgagcgggc ggcacctcgg cgggcgatgc tgcggtgcgg gcgaccctgg
     2281 tagcgttgc
//
LOCUS       DASWDC010000271         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_122351, whole genome shotgun sequence.
ACCESSION   DASWDC010000271 DASWDC010000000
VERSION     DASWDC010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122351"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="VF995_08750"
     CDS             complement(<1..147)
                     /locus_tag="VF995_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX9343686.1"
                     /translation="MSSERRDPLTYAAAGVDVEAGERAVSLMRAAVEGTHHRNVLGGI
                     GGFAG"
     gene            complement(166..1635)
                     /gene="purF"
                     /locus_tag="VF995_08755"
     CDS             complement(166..1635)
                     /gene="purF"
                     /locus_tag="VF995_08755"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX9343687.1"
                     /translation="MWRSMLEADQPDPSGPESPREACGVFGVWAPGERVANLVFYGLY
                     ALQHRGQESAGIAVADGDNLLVYKDMGLVSQVFDETTLATLQGHLAIGHTRYSTSGSS
                     RWENAQPTFKTTAHGGGIALGHNGNLTNTGALAATLPRRQDGTGATSDTDVMTELLAA
                     ERAAEVPLEEAILRVMPRLRGAYSLVIMDEHTLYGVRDPHGVRPLVVGKLPTGWVVAS
                     ETAALDIVGASFVREVEPGEAVMIDEAGLRSRRFAEASPNLCVFEYVYLARPDSTLGG
                     RSVHQVRRSLGAALAQEAPVEADLVVPVPDTAVSAAAGYAEASGVPFGEGLIKNRYVG
                     RTFIQPSQTMRQLGIRIKLNPLREAIRGKRLVVVDDSIVRGNTTKAIVTMLREAGAAE
                     VHLRITSPPVRWPCFYGIDMPTRGELIASGLLTDEVAAYTGADSLAYLSLEALTEASA
                     PQGGGLCSACFTGDYPIPLDTGSQGKLLLEPEPPLPKPS"
     gene            complement(1994..2458)
                     /locus_tag="VF995_08760"
     CDS             complement(1994..2458)
                     /locus_tag="VF995_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011278082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HEX9343688.1"
                     /translation="MNATSSSDKVVIRGEEFLRGAPMERVPGVRDLKLIYPETGFRPR
                     SLILGIVEIEPGCRSPLHRHNCEEAYYVLAGRGVVEHRDGATEYEVNAGDAVLNRENV
                     THRVRNAGTELLRLLVVGGIMFVPLVPNWPTDSPYEILEEREEGVTGASVVG"
     gene            complement(2415..2831)
                     /locus_tag="VF995_08765"
     CDS             complement(2415..2831)
                     /locus_tag="VF995_08765"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX9343689.1"
                     /translation="MRGACPDKPADTLAAHLAFPLGSAALSCGYLLAIGTANSIIGEL
                     MRQVMHALGEERRASLQVPGQIRRAIEGHRAIMEAIAAHDPTTAAQAMRQHLYDAQHY
                     ITDYIREFSPIPATGPSGNRREHERDQQFGQGGDPR"
BASE COUNT          404 a          986 c         1095 g          456 t
ORIGIN      
        1 cccggcgaac ccgccgatgc cgccgagcac attgcggtgg tgggttccct ccacggccgc
       61 gcgcatcagc gagaccgccc gctcccccgc ctcgacgtcg accccggcgg cggcgtaggt
      121 gagcgggtcg cggcgctcag agctcacgag ggcttcacga gggcttcacg agggcttcgg
      181 cagcggcggc tccggctcca ggagcagctt tccctggctg ccggtgtcga gcgggatcgg
      241 gtagtcgccg gtgaagcagg cgctgcagag gcccccgccc tggggtgcgg aggcttccgt
      301 cagggcctcc agcgagaggt aggcgaggga gtcggcgccg gtgtaggcgg cgacctcgtc
      361 ggtgagcagg ccgctggcga tcagctcgcc ccgggtgggc atgtcgatgc cgtagaagca
      421 gggccagcgc accgggggcg aggtgatgcg caggtgcacc tcggcggcgc ccgcctcgcg
      481 cagcatggtg acgatcgcct tggtggtgtt gccgcgcacg atcgagtcgt ccaccaccac
      541 caggcgcttg ccgcggatcg cctcgcgcag cgggttcagc ttgatgcgga tgccgagctg
      601 gcgcatggtc tggctcggct ggatgaaggt gcgtccgacg tagcggttct tgatcagccc
      661 ttcgccgaag gggacgccgc tcgcctcggc gtagccggcc gccgcgctga cggcggtgtc
      721 ggggaccggg acgaccaggt ccgcctcgac cggggcctcc tgggcgaggg cggcgccgag
      781 gctgcggcgc acctggtgga cgctgcgccc gcccagggtc gagtcggggc gggccagata
      841 gacgtactcg aagacgcaga ggttcggact ggcctcggcg aagcggcgcg atcgcaggcc
      901 ggcctcgtcg atcatcaccg cctcgcccgg ctcgacctcg cgcacgaacg aggcccccac
      961 gatgtcgagc gcggcggtct ccgaggcgac cacccagccg gtggggagct tgccgaccac
     1021 cagcggccgg acgccgtgcg ggtcgcggac cccgtacagg gtgtgctcgt ccatgatcac
     1081 cagcgagtag gcgccccgca ggcgtggcat gacgcgcagg atcgcctcct ccagcgggac
     1141 ctcggcggcg cgctcggcgg cgagcagctc ggtcatgacg tcggtgtcgc tggtcgcgcc
     1201 ggtgccgtcc tggcggcgcg gcagggtggc ggccagggcg ccggtgttgg tgaggttgcc
     1261 gttgtggccg agggcgatac cgccgccgtg ggcggtggtc ttgaaggtcg gctgggcgtt
     1321 ctcccagcgg gagcttccgc tggtcgagta gcgggtgtgc ccgatcgcca ggtgcccctg
     1381 cagggtggcc agggtggtct cgtcgaagac ctggctgacc aggcccatgt ccttgtagac
     1441 gagcaggttg tcgccgtcgg cgacggcgat cccagcggac tcctgcccgc ggtgctgcag
     1501 ggcgtagagg ccgtagaaca caaggttggc gacccgttca ccgggcgccc ataccccgaa
     1561 aacgccacaa gcttcccggg gggactcagg gcccgatggg tcaggctgat cagcttcgag
     1621 catgctcctc cacatgatgg atttgacgcg gcgggggaag ctcctccagc gtagccggat
     1681 cggggtcgcg gggccatcta cggagttcgc agtacgggca gatcgaccga gctcacgatg
     1741 acgggcgcga agcggccacg gatgctccgc gctggaggtg agacctgaat gaagaagttc
     1801 ctccgcaaga tgaagcgccg cctgcttggc ggcggcaaga agggctagca ggaccggaag
     1861 cctcagacgc ggggaagggc ccgcagccgc gaacccttcc ccgctctcgg tcgctcctgg
     1921 catggagcca cggtcgagcg tcccatcccc tcccgctctc ggtctgctcc cttgctgaca
     1981 ggggcggtgt cggctagccg acgacgcttg ctccggtcac gccctcttcg cgttcttcga
     2041 ggatctcgta gggcgagtcg gtcggccagt tcgggaccag cgggacgaac atgatcccgc
     2101 cgaccaccag caggcgcagc agctcggtgc cggcgttgcg gacccggtgg gtgacgttct
     2161 cgcggttcag gaccgcgtcg ccggcgttga cctcatattc ggtcgcccca tcgcggtgct
     2221 cgaccacgcc gcgaccggcg aggacgtagt acgcctcctc gcagttgtgc cggtgcagcg
     2281 gcgaacggca gccgggttcg atctcgacga tcccgaggat caggctgcgc gggcggaagc
     2341 cagtctccgg gtagatcagc ttgaggtccc ggacccccgg gacccgctcc atgggcgcgc
     2401 cgcggaggaa ctcctcaccg cggatcacca ccttgtccga actgctggtc gcgttcatgc
     2461 tccctccggt ttccactcgg tcccgtggct gggatgggac tgaactcgcg gatgtagtcc
     2521 gtgatgtagt gctgggcgtc gtacagatgc tggcgcatcg cctgggcggc ggtggtggga
     2581 tcgtgggcgg cgatcgcctc catgatcgcg cggtgcccct cgatcgcccg gcggatctgc
     2641 ccgggcacct ggagcgaggc gcggcgctcc tcgccgaggg catgcatcac ctggcgcatc
     2701 agctcgccga tgatcgagtt ggctgtgccg atggccagca ggtagccgca ggacagcgct
     2761 gcgcttccca ggggaaacgc gaggtgagcg gcgagcgtgt cggcgggttt atccggacat
     2821 gcgccgcgca ctatgctcca gcgctccagg gcctcggcgg ctgctcgtgc ccgggaggcg
     2881 caccgtcgcc gctccccggt cgaccggctg tagcaggcgc atccggccct gcccgcgtgt
     2941 g
//
LOCUS       DASWDC010000272         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1495197, whole genome shotgun sequence.
ACCESSION   DASWDC010000272 DASWDC010000000
VERSION     DASWDC010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495197"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VF995_08770"
     CDS             <1..713
                     /locus_tag="VF995_08770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX9343690.1"
                     /translation="TAGIAASPSPRSPDCVLLERGDPGALAAVREGRAVVQDPASALV
                     APALDPNPGDLVVDLCAGPGGKAGHLAALGATVLAVEPQPGRARLVADTARRLQLEGR
                     LHTVAADSRALPLVPGRADAALVDAPCSNLGSLRRRPEARWRHQPDEIEGLAALQLDL
                     VRAAAEAVRPGGSIVYSVCTWTARETDGVVDAALATIPGLEPAPLGGPAGPGASRQLW
                     PHRDDSDGLFLARLRKIV"
     gene            849..1526
                     /gene="rpe"
                     /locus_tag="VF995_08775"
     CDS             849..1526
                     /gene="rpe"
                     /locus_tag="VF995_08775"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX9343691.1"
                     /translation="MARRLAPSILNADLANLADQVRLVQDAADWIHIDIMDGHFVPNL
                     TFGPPTVAALRPHSDRFFDCHLMVDNPEALLPALADAGADSVTMHVEALDDPGRAIRA
                     ARGHGLGAGLALKPDTPLTDALGWLDAIDLLLPMTVEPGFGGQAFRADVLPKLAAARQ
                     AIDVLAGGRPVALEVDGGINADSVPRCLAAGVDTFVVGTAIFHAKDPAAAAKAFRAMI
                     HPVPEGA"
     gene            1539..2462
                     /locus_tag="VF995_08780"
     CDS             1539..2462
                     /locus_tag="VF995_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9343692.1"
                     /translation="MVLIADDDRDIVRFVEVNLRLEGFEVVTAHDGKDALNKALDLQP
                     NLILLDVMMPGMDGFEVCTKLRAADRTSHIPVIMLTAKSLSSDKVVGLTAGADDYIIK
                     PFDPMELVARVKTTLRRASEMRALSPLTGLPGNNRIEQELQARMKRETPTAVVYADLD
                     NFKSYNDRYGFLRGDEVISLAAQVLRRAAQDAAGSDGFVGHIGGDDFVAIVPPEVAEP
                     FAELAISTFDAQIVTLYDRADADAGSIEMVDRQGQLRRFPIVSISLGIASTARRQFAD
                     YRELVATATELKNLAKKRQGSSFELDRRTTL"
     regulatory      2525..2654
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2760..3449
                     /locus_tag="VF995_08785"
     CDS             2760..3449
                     /locus_tag="VF995_08785"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009713589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX9343693.1"
                     /translation="MFTGIVEGQGTVTALRFAPHGSAAVVEVEAGWLDSTLRVGDSVA
                     VNGCCLTATRVTGTGGDGAAGSGSGSGSGFAADVMAETLRRTALGRLAPGARVNLERP
                     LALGERLGGHLVQGHVDGLGTLLERRPGGQGSEVLRFGLDPALARYLVEKGSVALDGV
                     SLTVAAARPDWFEVALIPHTLAVTTLGPLAPGGVVNLEVDLLAKYVERLLQPHVEPRA
                     AGTGTGTEVAR"
     gene            3446..4714
                     /locus_tag="VF995_08790"
     CDS             3446..4714
                     /locus_tag="VF995_08790"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX9343694.1"
                     /translation="MTRFATIPEAVAAIRAGEMVVVVDNADRENEGDLIMAAEATTPA
                     KIAFYVRHTSGVIVFPMLGERLDELELPLMVTHNTEKHRTAFTVSVDAAHGTATGISA
                     ADRATTIQTLIDPNTKPADLNRPGHIFPLRYTEGGVLKRGGHTEAGIDLARLAGCYPA
                     AVLCEIVNPDGSMARLPELERFAATHHLKLITIADLIHSRRQSEKLVRRVAEARIPTR
                     WGDFTAYAYESILDGEQHLALVKGEVAGQAQLLVRVHSECLTGDAFGSLRCDCGSQLD
                     GALRRIAAAGQGVVVYLRGHEGRGIGLAHKLRAYHLQENGRDTVDANLELGLPVDDRE
                     YGIGSQILADLGVTTMRLMTNNPAKYRGLEGYGLQIVERVPLETYPNPENLHYLRTKR
                     ERMGHLLDLPADQAGAATETATETTAGEGV"
BASE COUNT          679 a         1857 c         1608 g          608 t
ORIGIN      
        1 ccaccgccgg gatcgccgcc tcgccgagcc cccgatcgcc cgactgcgtg ctgctggaac
       61 gcggcgaccc cggcgccctg gcggcggtcc gggagggccg cgcggtggtg caggaccctg
      121 cctccgcact ggtcgcgccc gccctcgacc ccaaccccgg cgacctggtc gtcgatctgt
      181 gcgccgggcc gggcggcaaa gccggccacc tcgccgcgct cggcgcgacc gtgctcgccg
      241 tcgagccgca acccggccgc gcccgcctgg tcgccgacac cgcccgccgc ctccagctgg
      301 aaggccgtct ccacaccgtg gccgccgaca gccgcgccct gccgctggtg ccgggccgcg
      361 ctgacgccgc cctggtcgac gcgccctgct ccaaccttgg gagcctccgc cgccgccccg
      421 aggcgcgctg gcgccaccag ccggacgaga tcgagggcct ggccgccctc cagctcgacc
      481 tggtccgggc cgccgccgag gcggtccggc caggcggcag catcgtctac tcggtctgca
      541 cctggaccgc ccgcgagacc gacggggtgg tcgacgccgc cctggcgacc attcccgggc
      601 tggagccggc gccgctcggc gggcccgccg gccccggcgc aagccgacaa ttgtggccgc
      661 atcgggacga cagcgacggt ctttttctcg ctcgactgcg caagatcgtc tgatcgcgtc
      721 gcgcgtcgcg cgcattcccc gcgtccgctc gataccatct gcgaaacccg gtccgggcgg
      781 gggagggcgc cgcccaaggg cgcgcccgag cccagggcat ccggaccatc ggatcgaact
      841 ggagtgtcgt ggcccgtcgc ctagccccct cgatcctcaa cgccgacctc gcgaacctgg
      901 ccgaccaggt ccgcctggtc caggacgccg ccgactggat ccacatcgac atcatggacg
      961 gacatttcgt gcccaacctc acgttcggcc cgccgacggt cgcggcgctc cgcccccata
     1021 gcgaccgctt cttcgactgc cacctgatgg tcgacaaccc cgaggcgctg cttccggccc
     1081 tggccgacgc cggcgccgac tcggtcacca tgcacgtcga ggcgctcgac gaccccggca
     1141 gggccatccg ggccgcccgc ggccatggcc tcggcgccgg gctggctctc aagccggaca
     1201 cgccgctcac cgacgccctc ggctggctcg acgccatcga cctgctgctg cccatgaccg
     1261 tcgagcccgg cttcgggggg caggcgttcc gcgccgacgt gctgcccaag ctcgccgccg
     1321 cccgccaggc gatcgacgtc ctcgcagggg gacgcccggt cgcgctcgag gtcgacggcg
     1381 gcatcaacgc cgactccgtg ccgcgctgcc ttgcggccgg tgtcgatacc ttcgtggtcg
     1441 ggaccgcgat cttccacgcc aaggacccgg cggcggcggc caaggcattc cgtgcgatga
     1501 tccacccggt accggaaggg gcttgaaccg tggacgagat ggtgctgatc gccgacgacg
     1561 atcgtgacat cgtccgcttc gtggaggtga acctccggct ggagggcttc gaggtggtca
     1621 ccgcccacga cggcaaggac gccctcaaca aagccctcga cctgcagccc aacctgatcc
     1681 tgctggacgt gatgatgccc gggatggacg ggttcgaggt ctgcaccaag ctccgcgccg
     1741 ccgaccggac ctcgcacatc ccggtgatca tgctgaccgc gaaatcgctg tcgtccgaca
     1801 aggtggtcgg cctgaccgcc ggcgccgacg actacatcat caagccgttc gacccgatgg
     1861 agctggtcgc ccgggtcaag accaccctgc gccgcgccag cgagatgcgc gccctgtcgc
     1921 cgctcaccgg cctgcccggc aacaaccgca tcgagcagga gctgcaggcc cgcatgaagc
     1981 gggagacccc caccgcggtg gtctacgccg acctggacaa cttcaagtcc tacaacgacc
     2041 gctacgggtt cctccgcggc gacgaggtga tctcgctggc cgcgcaggtg ctgcgccgcg
     2101 ccgcgcagga cgccgccggc agcgacggct tcgtcggcca catcggcggc gacgacttcg
     2161 tggcgatcgt cccgccggag gtcgccgagc ccttcgccga gctggcgatc agcaccttcg
     2221 acgcccagat cgtgaccctc tacgaccgcg ccgacgccga cgccggcagc atcgagatgg
     2281 tcgaccgcca gggccagctg cgccgcttcc cgatcgtgtc gatctcgctc gggatcgcct
     2341 cgaccgcccg ccgccagttc gccgactacc gcgagctggt cgccaccgcc accgagctga
     2401 agaacctcgc caagaagcgc caggggtcaa gcttcgagct ggaccgccgc accaccctct
     2461 agacgcctgg cagccggctc cggcggcgtg ttgccggccg gtgttaccct gctggcagga
     2521 aagcgcacgt ctccgaggcg gggtgaaagt ccccaccggc ggtcacagcc cgcaaccccc
     2581 ggccgatcct ggccggggct gaccctgtga aacccagggg ccgacggtca cagtccggat
     2641 gggaggaggc gggaggccgg cgacccggcc ctggtccgcg ttcgccgcca acgcgaccag
     2701 tcggtctggt tcgtccaggt ttgccgcgac gccccggagt tgccggaagg ggtgtcgcgg
     2761 tgttcaccgg gatcgtcgag gggcagggga ccgtgacggc gctgcggttc gcgccgcacg
     2821 gcagcgctgc cgtggtcgaa gtcgaggccg gctggctgga cagcacgctg cgggtcggcg
     2881 actcggtggc ggtcaacggc tgctgcctga ccgccacccg cgtcaccggg accggcggtg
     2941 acggcgccgc cgggtccggg tccgggtccg ggtccggatt cgcggccgac gtgatggccg
     3001 aaacccttcg gcgcaccgcc ctcgggcggc tggcgcccgg cgcgcgggtc aacctggagc
     3061 ggcccctggc actcggcgag cgcctcggcg gccacctcgt ccagggccac gtggacggcc
     3121 tgggaaccct gctggagcgc cggccgggcg gccagggcag cgaggtgctg cgcttcgggc
     3181 tcgacccggc gctggcccgc tacctggtcg agaagggctc ggtcgccctc gacggggtca
     3241 gcctgacggt ggccgccgcc cggccggact ggttcgaggt cgcgctgatc ccgcacaccc
     3301 tggcggtgac gaccctcggc ccgctggcgc ccggcggggt cgtcaacctc gaggtcgacc
     3361 tgctcgccaa gtacgtggaa cggcttctcc aaccacacgt cgagccgcgg gcggcgggga
     3421 cgggaacggg aacggaggtg gcccggtgac ccggttcgcg accatccccg aggcggtcgc
     3481 ggcgatccgc gccggcgaga tggtggtggt ggtcgacaac gccgaccgcg agaacgaggg
     3541 tgacctgatc atggcggcgg aggcgaccac ccccgccaag atcgccttct acgtgcgcca
     3601 cacctccggg gtgatcgtct tccccatgct cggcgagcgg ctcgacgagc tggaactgcc
     3661 gctgatggtc acccacaaca ccgagaagca ccgcaccgcc ttcaccgtct cggtcgacgc
     3721 cgcccacggc accgccacgg gcatctccgc cgccgaccgc gccaccacca tccagacgct
     3781 gatcgacccc aacaccaagc ccgccgacct caaccgcccc gggcacatct tcccgctgcg
     3841 ctacaccgag ggcggggtgc tcaagcgcgg cggccacacc gaggccggca tcgacctggc
     3901 gcggctcgcc ggctgctacc cggcggcggt gctgtgcgag atcgtcaacc ccgacggcag
     3961 catggcccgc ctccccgagc tggagcgctt cgccgccacc caccacctca agctgatcac
     4021 catcgccgac ctgatccact cccgccgcca gagcgagaag ctggtccgcc gcgtcgccga
     4081 ggcgcgcatc cccacccgct ggggcgactt caccgcctac gcctacgagt cgatcctcga
     4141 cggcgagcag cacctggccc tggtcaaggg cgaggtcgcc ggtcaggccc agctgttggt
     4201 ccgggtccac tccgagtgcc tcaccggcga cgccttcggc agcctccgct gtgactgcgg
     4261 ctcgcagctg gacggcgcgc tgcgccgcat cgccgccgcc ggccagggcg tggtcgtcta
     4321 cctgcgcggc cacgagggcc gcggcatcgg cctggcccac aagctgcgcg cctaccacct
     4381 gcaggagaac ggccgcgaca ccgtcgacgc caacctggag ctgggcctgc cggtcgacga
     4441 ccgcgagtac gggatcgggt cgcagatcct cgccgacctc ggcgtcacca ccatgcggct
     4501 gatgaccaac aaccccgcca agtaccgcgg cctggaaggc tacggcctgc agatcgtcga
     4561 gcgggtcccg ctggagacct acccgaaccc cgagaacctc cactacctgc gcaccaagcg
     4621 ggagcgcatg ggccacctgc tcgatctgcc ggccgaccag gccggcgccg cgaccgagac
     4681 cgcgaccgag accacggccg gggagggcgt atgacccgca cctacgaagg cggcatgcgc
     4741 ggcgacggcc gc
//
LOCUS       DASWDC010000273         5269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_958370, whole genome shotgun sequence.
ACCESSION   DASWDC010000273 DASWDC010000000
VERSION     DASWDC010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958370"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /locus_tag="VF995_08795"
     CDS             complement(<1..1615)
                     /locus_tag="VF995_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEX9343695.1"
                     /translation="MSGKLPTPQRSSEQFLLVVMLITFGALVTLWLTGEVGGYLASGH
                     WPAMSLSEMGGVLIGFFGHPSDPAHAWPAAVRGQLPSPVMFYAVLMVLLLLATTAWLY
                     LGMVLRWLRRRTGAVAPARQVSPAGQTLPSGWARPQLFRDLFVRAPEAGRVTLGRTNG
                     RLVAAGASQPVVVLGPASSHKTSGMVVPAILEWSGPVLAAGMNPDFIRHTMSQRWQRG
                     EVHLFDPAGVTGLDSSNWSPLGRCGAWEGAYRMARSLVDTSRRGSQPSPDGDFLHNAA
                     VALLAALLLAAATSDRSMGDVARWVQRQEHAEVAAALSMAGEPAARDALQLVSGLTSG
                     HRGQVYAAVLAVIAAYADPALKQTVLAAQFSADRLLDGHANTAYVCAPAHEQRRLGPV
                     FTALVQEVVDLAYERSREQGKPLDPPLLLLLDDAATAVALPGLDALAATAASHGVQLL
                     TTFRHVSQIQARYGDRAETILSSHRAKVILSGVTDQPTLTVLSHLLEDETIRSLATPA
                     TLAKAGKDDQDAALRARYPSPAETLHRIWP"
     gene            complement(1670..2716)
                     /locus_tag="VF995_08800"
     CDS             complement(1670..2716)
                     /locus_tag="VF995_08800"
                     /inference="COORDINATES: protein motif:HMM:NF040179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9343696.1"
                     /translation="MLFGRTAWVIDPKGEYNRLAAYCGVEPIRFATGGMLRLNPLDPM
                     ISGQSQLALLRSIGTVMLGRNLRPEEDACLERAHHEAVIEASNRGIQPVVPQVVNRLL
                     DPSDATAHELRASMGRLAEDGRQLALALRRMGAGDLRGMFDGPTSRGIDLTGRLVVFN
                     LREVKDEARPILMACTAAWLQGAWARNDGVRRIVVLDEAWVVLRHLAIARWLRESWKL
                     ARQYGVQNIAVMHRLSDLTAAGDAGSEQVQLAKGLLEDSETRVIYRQSPGEVEQAREL
                     LGLTQTEVEQIPTLERGEALWRVGRRSFIVQHRIDQVIEREIVDTDANMDGRPPPWAE
                     PESDDAQAISGGAS"
     gene            complement(2962..4473)
                     /locus_tag="VF995_08805"
     CDS             complement(2962..4473)
                     /locus_tag="VF995_08805"
                     /inference="COORDINATES: protein motif:HMM:NF042935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO6880 family protein"
                     /protein_id="HEX9343697.1"
                     /translation="MRNTASHVDYRYRFGPLERRGLIGSLRAGQVAVLGVSVALVIMI
                     VEAIRNGIGLAIGAMILGVGMLGTFVPFGGRTLNEWAPVMASFLGRRLSGRDRYRSGA
                     AEAGTRFQGGDVPVKLPDPIGKIELIAFPFRGVELGVIADRANGTFTAVIQARARSFV
                     LLDPADKAYRLAGWAGVVAGLAREGSPISRLQWIERTVPDDRNALSRYLRDAIDPSIG
                     IDALPLQSYLRLTHGAGPVTEQHELYIAMQVSASKAHRLIRQAGGRDTGACTVLAREL
                     ETMARRLESAEVEVLHALGPRRLAATIRLAYDPHARTNLARLATADSSREEGVSARNA
                     WPQRTQTYWSHYRTNDVVHATYWIAEWPRMEVGPDFLAPLLVQTRCMRTVSVTMEPVP
                     PLKAMRAVGFAKTADVADEELRQKLGFLGTAKRRNQADAVARREQDLADGHADVRFSG
                     YITVTADDTDSLLEACGEVEHAAGQSRLELQRCDGEQDIAFTFSALPLCRGLR"
     gene            complement(4466..>5269)
                     /locus_tag="VF995_08810"
     CDS             complement(4466..>5269)
                     /locus_tag="VF995_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343698.1"
                     /translation="IGAGTANPAVPVFGVLLGAMVVVLGAFSIWLELLLRSAAIYIAV
                     LFLPIGLSAMIWPSGWRWCKRLIDFLIAIVFAKVFIVAIINLAASGLAQGGFADKFEG
                     VLAGGALLLMAAFTPIALLRLIPLAEAAVVSAGQQRAALRQATQGATAFTGTSVVSQM
                     IQSRFRGAGGGAGSGWAGGAGAASGAASNAVSGARQRVNSGFATMTPDGGGLPEGGAR
                     PVPPPPKPEVGPAPSSNGSGSSGGGFGGGMARPLKLPELAPEGGVGSDA"
BASE COUNT          770 a         1821 c         1972 g          706 t
ORIGIN      
        1 cgggccagat gcggtggagg gtctcggctg gggacggata gcgggcgcgc agggccgcgt
       61 cctggtcgtc cttgccggcc ttggcgaggg tggcgggggt ggcgaggctg cggatcgtct
      121 cgtcctccag caggtgggac aggaccgtga gggtgggctg gtcggtgacg cccgacagga
      181 tcaccttggc gcggtggctg gagaggatcg tctcggcgcg gtcgccgtag cgggcctgga
      241 tctgggagac gtggcggaag gtggtgagca gctggacgcc gtggctggcg gcggtggcgg
      301 cgagcgcgtc caggccgggc agggccaccg cggtggcggc gtcgtcgagc agcagcagca
      361 gcggcgggtc caggggtttg ccctgctcgc gggagcgttc gtaggcgagg tcgacgacct
      421 cctgcaccag tgcggtgaag accgggccga ggcggcgctg ctcgtgcgcg ggcgcgcaga
      481 cgtaggcggt gttggcgtgg ccgtcgagca accggtcggc ggagaactgc gcggcgagca
      541 cggtctgctt gagcgccggg tcggcgtagg cggcgatcac cgccagcacc gccgcgtaca
      601 cctgcccgcg gtggccgctg gtgaggcccg agacaagctg gagcgcgtcg cgggcggcgg
      661 gctcgccggc catgctgagc gcggcggcga cctcggcgtg ctcctggcgc tgcacccagc
      721 gggccacgtc gcccatggag cggtcggagg tggcggcggc gagcagcagc gccgccagca
      781 gcgccacggc ggcgttgtgc aggaagtccc cgtcgggaga gggctggctg ccgcgccggg
      841 aggtgtccac cagcgagcgg gccatccggt aggcgccctc ccaggcgccg cagcggccca
      901 gcggcgacca gttggagctg tccaggccgg tgacgcccgc cgggtcgaac aggtgcacct
      961 cgccgcgctg ccagcgctgg ctcatggtgt ggcggatgaa gtccgggttc atcccggcgg
     1021 ccagcaccgg accgctccat tcgaggatcg ccggcaccac catgccgctg gtcttgtggc
     1081 tggaggccgg gccgagcacc accaccggct gggacgcccc ggcggcgacc agccgaccgt
     1141 tggtccgccc gagggtgacc cgcccggcct ccggcgcgcg cacgaacagg tcgcggaaca
     1201 gctgcggccg cgcccacccc gacgggaggg tctggccggc gggcgagacc tggcgggcgg
     1261 gcgccaccgc gcccgtgcgg cgccgcagcc agcgcagcac catccccagg tacagccagg
     1321 cggtggtggc gagcagcagc aggaccatca ggacggcgta gaacatcact gggctcggca
     1381 gctggccgcg gaccgccgcc ggccaggcgt gcgccgggtc gctcggatgc ccgaagaacc
     1441 cgatcagcac cccgcccatc tcgctcagcg acatcgccgg ccagtggccc gaggccaggt
     1501 agccgccgac ctccccggtg agccacagcg tcaccagcgc cccgaaggtg atcagcatca
     1561 ccaccagcag gaactgctcg ctggagcgct gcggcgttgg aagcttccca ctcatggacg
     1621 acgcaccgcg ctcatggttc gccgcttccg gtgcgcccgc ccaagtgcct catgatgccc
     1681 cgccgctgat cgcctgggcg tcgtcggact ccggttccgc ccacggcggc gggcggccat
     1741 ccatgttggc gtcggtgtcg acgatctccc gctcgatcac ctggtcgatg cggtgctgca
     1801 cgatgaacga gcggcgcccg acccgccaca gcgcctcgcc gcgctccagc gtggggatct
     1861 gctccacctc ggtctgggtg agaccgagca gctcccgggc ctgctccacc tcgccgggcg
     1921 actgccggta gatcacccgc gtctcggagt cctccaggag gcccttggcg agctgcacct
     1981 gctcgctgcc ggcgtccccg gcggcggtca ggtccgacag ccggtgcatc accgcgatgt
     2041 tctgcacgcc gtactggcgc gcgagcttcc agctctcccg cagccagcgc gcgatcgcca
     2101 ggtgccgcag caccacccac gcctcgtcga gcaccacgat gcgccgcact ccgtcgttgc
     2161 gcgcccacgc cccctgcagc cacgcggccg tgcaggccat caggatcggc cgcgcctcgt
     2221 ccttgacctc ccgcaggttg aacaccacca gccgcccggt caggtcgatc ccccgcgagg
     2281 tcggcccatc gaacatcccc cgcaggtccc cagcccccat ccttcgcaac gccagcgcca
     2341 gctgccgccc gtcctccgcc agccgcccca tcgaagcccg caactcatgc gcggtcgcat
     2401 ccgaaggatc cagcaaccgg ttcaccacct gcggcacgac cggctgtatc ccccggttgg
     2461 acgcctcgat caccgcctca tgatgcgccc gctccaggca ggcgtcctcc tcaggccgca
     2521 ggttccgccc aagcatcacg gtcccaatgg accgcagcag ggcgagctgc gactgtccgg
     2581 agatcatcgg atcgagcggg ttgagccgca gcatgccgcc ggtcgcgaac cggatcggct
     2641 cgacgccgca gtaggccgcc agccggttgt attcgccctt cgggtcgatg acccaggcgg
     2701 tgcgaccgaa gagcatactt ctaaaaagga acgttttgac caagctcgac ttagcatacc
     2761 caatctgccc aattacgagc atatttgggc ccgtgatctc acgcccgtaa agagagaagc
     2821 tgtcgtaaca gaaacttcca ccgaagacat cacgaccgat gtagacgcct tcactgccaa
     2881 gaccgccttc tgagacaaat gggtatgcgg cctgaaggtg agcggtcgtg acccgatggg
     2941 ctggccggcg cgctctggtc ctcatcgcaa gcctcggcaa aggggcagcg cagagaacgt
     3001 gaatgcgatg tcctgctccc cgtcgcagcg ctgcaattcg aggcgggact ggccggcggc
     3061 gtgttcgact tcgccgcagg cttcgaggag ggaatcggtg tcgtcggcgg tgacggtgat
     3121 gtagccggag aagcggacgt cggcgtggcc gtcggcgagg tcctgttcgc ggcgggcgac
     3181 cgcgtccgcc tggttgcggc gcttggcggt gccgaggaag ccgagcttct ggcggagctc
     3241 ctcgtcggcg acgtcggcgg tcttggcgaa gccgacggcg cgcatggcct tcagcggcgg
     3301 gaccggctcc atggtgaccg agaccgtgcg catgcagcgg gtctggacca ggaggggggc
     3361 gaggaagtcg gggccgacct ccatgcgggg ccattcggcg atccagtagg tggcgtggac
     3421 cacgtcgttg gtgcggtagt ggctccagta ggtctgggtg cgctggggcc aggcgttgcg
     3481 ggcgctgacg ccctcctcgc ggctggagtc ggcggtggcg aggcgggcga ggttggtgcg
     3541 ggcgtggggg tcgtaggcga ggcggatggt ggcggccagg cggcgcgggc cgagggcgtg
     3601 gaggacctcg acctcggcgg actccaggcg gcgggccatg gtttcgagct cgcgggcgag
     3661 gacggtgcag gcgccggtgt cgcggcctcc ggcctggcgg atcaggcggt gggccttgga
     3721 ggctgagacc tgcatggcga tgtagagctc gtgctgctcg gtgaccgggc cggcgccgtg
     3781 ggtgaggcgc aggtagctct gcagcggcag cgcgtcgatg ccgatcgacg ggtcgatcgc
     3841 gtcgcgcagg tagcgggaga gggcgttgcg gtcgtcgggg acggtccgct cgatccactg
     3901 cagccggctg atggggctgc cctcgcgggc gaggccggcg accacgccgg cccagccggc
     3961 caggcggtag gccttgtcgg ccgggtcgag cagcacgaag gagcgcgccc gcgcctggat
     4021 cacggcggtg aaggtgccgt tggcgcggtc ggcgatcacg cccagctcga cgccgcggaa
     4081 ggggaaggcg atcagttcga tcttgccaat tggatcgggc agcttgaccg ggacgtcgcc
     4141 gccctggaag cgggtgccgg cctcggccgc acccgagcgg tagcggtcgc ggccagagag
     4201 cctgcggccg aggaacgatg ccatcaccgg tgcccactca ttcagtgtcc tgcctccgaa
     4261 gggcacgaag gtgcccagca tgccgacgcc gaggatcatg gcgccgatcg ccagcccgat
     4321 gccgttgcgg atcgcttcga cgatcatgat gaccagcgcc accgacaccc cgaggacggc
     4381 gacctggccg gctcggaggc tgccgatcaa cccgcggcgc tccagcgggc cgaagcggta
     4441 gcggtagtcg acgtggctgg ccgtgttacg catccgatcc gactccgccc tctggggcca
     4501 gctcgggcag cttcagcgga cgggccatgc cgccgccgaa gccgccgccg ctggaaccgg
     4561 acccgttcga cgacggggcg ggcccaacct ccggcttggg gggtggcggc accggccggg
     4621 cgccgccctc gggaaggccg ccgccgtcgg gggtcatggt cgcgaagccg gagttgactc
     4681 gctggcgcgc gcctgagacc gcgttggagg ccgcgccact ggccgcgcca gccccgccgg
     4741 cccagcccga gccggcgccg ccgccggcgc cgcggaagcg ggactggatc atctgcgaca
     4801 ccaccgaggt gccggtgaag gcggtggcgc cctgggtcgc ctggcggagc gcggcgcgct
     4861 gctggccggc gctgaccacc gccgcctcgg ccagcgggat gagccgcagc agcgcgatcg
     4921 gggtgaaggc ggccatcagc agcagggcgc cgccggccag cacgccctcg aacttgtcgg
     4981 cgaagccgcc ctgggcgagg ccggaggcgg ccaggttgat gatcgccacg atgaacacct
     5041 tggcgaagac gatcgcgatc aggaagtcga tcagccgctt gcaccagcgc cagccggatg
     5101 gccagatcat cgccgacagg ccgatcggca ggaacagcac ggcgatgtag atcgcggccg
     5161 agcgcagcag cagctccagc cagatcgaga aggcgccgag caccaccacc atcgcgccga
     5221 gcaacacgcc gaacacgggc acggccgggt tggcggtgcc ggcgccgat
//
LOCUS       DASWDC010000274         9310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1329280, whole genome shotgun sequence.
ACCESSION   DASWDC010000274 DASWDC010000000
VERSION     DASWDC010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329280"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VF995_08815"
     CDS             <1..253
                     /locus_tag="VF995_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343699.1"
                     /translation="MGTDRVLQATLDRAAHLDLQVALLPALRDLDRVEDLHAALEGGE
                     LDRAPRTRAAAWELLAAAPGHGRGLCDGAGALSARGSSR"
     gene            complement(260..904)
                     /locus_tag="VF995_08820"
     CDS             complement(260..904)
                     /locus_tag="VF995_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9343700.1"
                     /translation="MVQRVPYRRGLRVLETSPRLGDDYRQAMRRRVDYLASDYDERAH
                     RTELKLDLTRMELADGSLDLAMTAHVLEHIPDTDAALRELHRVLRPGGRALIQVPLVQ
                     PATAPPVEPEFHEDNTPVFWRFGFDLTERLRDHGFTATVLVTQDFRRRVLSLDTDWPE
                     GISGEFDAIGMVKAADPADLTVVASDEVAERSWLRPSFMHAAWECVKPVGVEGQ"
     gene            complement(1609..1809)
                     /locus_tag="VF995_08825"
     CDS             complement(1609..1809)
                     /locus_tag="VF995_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343701.1"
                     /translation="MGEIVYRSEVSVTRQRGPLRTATLPALEEPVVFGVHDAVAEHYG
                     IEPDEQEPHTTTLDYLVAAAAG"
     gene            complement(1889..2341)
                     /locus_tag="VF995_08830"
     CDS             complement(1889..2341)
                     /locus_tag="VF995_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9343702.1"
                     /translation="MPEPLPVVIADTSALLSFFNDTDAHHQAVWDGFTTVGHVVVSPC
                     VLTELDYLIAKRQGPTPARAVLGFITDLVATGRWEVPDIGPFLLVAHAVLGDYPAIGL
                     ADAINVALAREFHTDVVATLDRRHFRMIRPLSRHDSFRLLPDDVPAGS"
     gene            complement(2334..2591)
                     /locus_tag="VF995_08835"
     CDS             complement(2334..2591)
                     /locus_tag="VF995_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007460476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9343703.1"
                     /translation="MEATARELNQQTAQVLARVEAGETVIVTKNGRPIAVVRPYGPDD
                     PPVYPFRTDPIGHYDAVPTFSGPPDLSERADEFLEGFGEDA"
     gene            complement(2637..3368)
                     /locus_tag="VF995_08840"
     CDS             complement(2637..3368)
                     /locus_tag="VF995_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018089478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX9343704.1"
                     /translation="MTGGRADINTITEVWHRVVGTQPDPPTWLVLACGLLALAAVVPD
                     RAWRVARNVATITHEGGHALAALVTGRRLGGIRLHADTSGVTVSAGRPTGPGMVLTSA
                     AGYIASPLLGLGGAWLLAAGHVTALLWISLVLLGGMLVLIRNPFGAATVLAAGAAVFL
                     ISWLTPASVQAAFAYLLTWFLLLGGLRPVVELQHERRRRRAPNSDADQLARLTRVPGL
                     VWVALFTLTALAAILAGGRWLLHLG"
     gene            3469..4119
                     /locus_tag="VF995_08845"
     CDS             3469..4119
                     /locus_tag="VF995_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343705.1"
                     /translation="MTDWATISSLATAGATLALAVATFSSVRSANRAARTAERALEAR
                     LRPLLLSSRLEDPTQKIIWADEHKTTVGGGRAAVEVGADGTIYLAMSVRNVGAGMAVL
                     HGWYPRPEWLSGIPAPSDVRHEDPERFRRLTRDLFVPAGDFGFWQGAIRDADDPLREG
                     LPDAIAARRRFGIDVLYGDHEGGQRIISRFTLAPAADSSWLCSVARHWNLDRPDPR"
     gene            complement(4121..5722)
                     /locus_tag="VF995_08850"
     CDS             complement(4121..5722)
                     /locus_tag="VF995_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020131079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9343706.1"
                     /translation="MGERRHNLAERLATLAARSGWRDRSAFLVGDERYTHGAVHDGAA
                     RTAALLARHGVGPGDRVLIALPDGVEFAWAFLGAARLGAIAVPVNPQLAAADHAAMLE
                     DATPSTVVCGDELAGRFAPVPVLSAGGLAAGIRQVEPAPAAPVTPETPAYAQFTSGTT
                     GRPKAALHRHRDLEVYFQAFARRALRVREDDVLFSASKLYFAYGLGNSLAFPLLSGAS
                     AVLHPGRPRPEAIGALLERHQPTILFAVPTLYANLLAAVAGGRPPTRDLRLAVSAGEA
                     LLPALAERVRAAFGCLVLDGLGSTEVGQTFVSNTPDAWRDGTVGRALPPYQVEVRAGG
                     DAGGEAARPCPPAEHAGERSDAVRGRPAEPGEQGVLWVNGPTVLLGYWNNPEATAAAL
                     HGEWLRSGDLAVLDDDGFVRMAGRADDIENVGGINLAPAEIEGLLATHDGVTEVAVAA
                     VRDPNGATHLRAFVVAGPAHGGEAARTALEAELLARCRAELTAFKVPRAVTFLEALPR
                     TPTGKLRRFMLRAWQPEADPEERPG"
     gene            complement(5755..7743)
                     /locus_tag="VF995_08855"
     CDS             complement(5755..7743)
                     /locus_tag="VF995_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX9343707.1"
                     /translation="MESRGFVALQRSYPYQPALDGLRALAVAAVLLYHGQVGWVPGGF
                     LGVDLFFVLSGYLITTLLLAEWEGRGGIDLKGFWARRARRLLPALFLMLAAVAAYGAL
                     LAPAATRARLRWDGLATLGYAANWRFAISHLPYFAQFGDPSPLTHMWSLGIEEQYYLL
                     WPLLLLGLLGLAGRRARRGLPTPLLGLVLLGALGSALLMALLYQPGTDPSRVYYGTDT
                     RAQALLLGGALAIALARLGRPLPRVLADSLGVAGLGGLAALMLRVPDTATWLYRGGFA
                     LAAVAAAGAIVAAVQPEGDGRVRELLSLAPLPAIGRISYGLYLWHWPVDVALSPERTR
                     LGPVPLLAVRLAATFALALLSYFLLEQPIRQGALRRRGLARPATAGAVVGLAALLVLT
                     TAAGPGAGAEGATLRAGTGPAGLPTVPPPGSGTGAGQAPGSGGNGFRVFLVGDSVAFT
                     LGYNYQQGAVPRMTLGGDTDIGCGLARAPSVNQDEGAVQAVDPKCTTWPTRWRADAAA
                     FHPDVSVLLIGAWEVLDKRVGGRTLRAGSTDDERYLDSELQLAIDTLAPVSRRIAILN
                     VPCFQEPQTADAYAWIAKARDDPARGVWLNQVLGRFAAAHRDRVTVLPLRDFLCPGGQ
                     QDPANGTKVRYDGVHFTADGATLVWDWLGPQLYSLAKQ"
     gene            complement(7834..8178)
                     /gene="cutA"
                     /locus_tag="VF995_08860"
     CDS             complement(7834..8178)
                     /gene="cutA"
                     /locus_tag="VF995_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004955897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX9343708.1"
                     /translation="MVEQGKGKGIVPLQVTVAVPDEAAARRLAEGIVTERLAAAVQVV
                     GPVWSVFWWQGERHRADEWLCLAKTTAARFQRLAAYVREHHAYEVPEVIAVPVSDGNA
                     DYLAWLGEETGA"
     gene            complement(8180..9148)
                     /locus_tag="VF995_08865"
     CDS             complement(8180..9148)
                     /locus_tag="VF995_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9343709.1"
                     /translation="MRYRTLGRDGPALSVVGFGAWAIGGPWAWGWGRQDDRQSIAALH
                     RALDAGVNWIDTAPAYGLGHSELLVGRVLRQRADRDRIVVATKCGLWADGRRDLYDLR
                     PQTIRREVDESLRRLGAEAIDLFLIHWPDRQTGTPIEASWAAMAALADAGKVRRLGVS
                     NFDVALLERCERIRHVDALQPPLSLIDRSALEVLLPWCVAHGTGAICYSPMQSGLLSG
                     AFSEQRLAELARDDWRRRDEQFQEPRLSANLAVAESLRPIAARYGVSVAVVAVAWVAA
                     QTGVTAAIVGARKASQVDGWLGAADLVLDADDLAALDGAVPGTLVG"
BASE COUNT         1369 a         3340 c         3403 g         1198 t
ORIGIN      
        1 gatgggcacc gaccgcgtcc tccaggccac cctcgaccgc gccgcgcacc tcgacctgca
       61 ggtggcgctg ctgcccgcgc tccgcgacct cgaccgcgtc gaggacctcc atgccgcgct
      121 ggagggcggc gagctggacc gcgccccccg cacccgcgcc gccgcttggg agctgctcgc
      181 cgcggctccc gggcacgggc gtggcctctg cgacggcgcc ggtgcgctca gcgcccgggg
      241 atcatcccgg tgagcgccgc tactgaccct cgacaccgac gggtttcacg cactcccagg
      301 cggcgtgcat aaagctgggc cgcagccagc ttcgctcagc gacctcgtcg ctggcgacca
      361 cggtgaggtc agccggatcg gcggccttga ccatcccgat cgcgtcgaac tcccctgaga
      421 tcccctcggg ccagtcggtg tccagactca gcacccggcg acggaagtcc tgcgtgacca
      481 gcaccgtcgc cgtgaagccg tgatcccgca gccgctcggt caggtcgaag ccgaaccgcc
      541 agaacaccgg ggtgttgtcc tcgtgaaact ctggctcgac tggcggcgcg gtcgctggct
      601 gcaccagagg aacctggatc aacgcccgcc cgcctggacg aagcacccga tggagctcgc
      661 gcaaggcagc gtcggtgtca gggatatgct cgaggacgtg cgcggtcatc gccagatcga
      721 gcgacccgtc ggcaagctcc atccgcgtca gatcaagctt gagctcggtc ctgtgggcgc
      781 gctcgtcgta gtcactggct aggtagtcca cccggcgtct catggcctgg cggtagtcgt
      841 ccccgagccg cgggcttgtc tccaggacgc gcaggccgcg ccggtacggc acccgctgca
      901 ccatgcagag gtagaggaac cggtcgcggc caaccgcgcc gcagcgcggg catttcgcgg
      961 actcgctgtg gagccctccc tcgaactgtt caccgcgcca gccgcagagc agacaccagc
     1021 agccccgctt cggcacgtct cggttggtca gcggccgcca aggccgatag cggatcggcc
     1081 gtcgcaacct cccccgagcg atctcactcg tcttgcgcag gtagtggagc gtgagattcc
     1141 gcgcggcggc agcatacgac atcgacgggc tccttgggat cggcaggcga cttgccgaga
     1201 gctcacgaag agaagacctc tagccaccat tcaactttat tgcgggcgaa tgagcaaaat
     1261 atcgccatta cgcacactct cccgaaggga gcccatcaag ttcgcgatgc tggtgtcggt
     1321 ctggctcagc gctcttcaac cagatccaag ctggtggaga tgtcgaccca gccgccgatc
     1381 gtcccgggcg accgggcaga agtcggcgtg gaagccgaac acccgctcga tcttggtccg
     1441 ctggtcgtca tcaacgccgt cggcacgcag ctggtaggtg acgtggatcc gcttgacaac
     1501 caggaccttg ccgtccagtt cgatctcgcc gaccgcgtct gtctgaagac gaccgtctcc
     1561 agaggagata cccttggcct caagggcacc cccgaaggtg cgagtcagtc acccggcggc
     1621 agcggccacc aggtagtcga gggtggtggt gtggggctcc tgctcgtcag gctcgatgcc
     1681 gtagtgctcg gcgaccgcgt cgtgcacgcc gaacaccacc ggctcctcca gggccggcag
     1741 cgtcgcggtc cgcaacggcc cccgctgccg cgtgacgctc acctctgagc ggtagacaat
     1801 ctctcccatc gcgacttcct ctctgctcgc ggcggcttcc cactgactct ttgaatgagc
     1861 tctctcacca gtgatgacga tggggcgatc aactgcctgc cggtacatcg tccggaagca
     1921 gcctgaacga atcatgccgg ctgaggggac ggatcattcg gaaatggcgg cgatccaggg
     1981 tagcgacaac gtcggtgtgg aactcacggg cgagtgcgac gttgatggca tccgcgaggc
     2041 cgatggcggg atagtcgccg agcaccgcgt gagcgacgag caagaatggg ccaatgtcag
     2101 gaacctccca acgtccggtc gcgaccagat cagtgatgaa ccccaggacc gccctcgccg
     2161 gagtcggtcc ctgccgtttg gcgatcaggt agtcgagttc ggtgaggacg cacggcgaga
     2221 caacgacatg accaaccgtc gtgaagccat cccagacggc ctgatggtgc gcgtcggtgt
     2281 cattgaagaa cgagagcagg gcggacgtat ccgcgattac gaccggaagg ggctcaggca
     2341 tcctcgccga atccctcgag gaactcatcg gcccgctcgg acaagtcggg gggtccgctg
     2401 aatgtcggga cagcgtcgta gtgacctatc gggtcagtgc ggaacgggta aaccggtgga
     2461 tcgtccggac catacggtcg cacaacggcg ataggccggc cgttcttggt gacgatgacg
     2521 gtctccccgg cctcgacacg ggcgaggacc tgcgcggtct gctggttgag ctctcgggct
     2581 gtggcttcca tgaacctact gtactacgcg tagtacgcct atgccggcaa gcaccgctag
     2641 cctaaatgca gcagccagcg gccgccggcg aggatcgcgg cgagggcggt gagggtgaac
     2701 agggcgaccc agaccaggcc ggggacgcgg gtgaggcggg cgagctggtc ggcgtcggag
     2761 ttgggggcgc ggcggcggcg gcgctcgtgc tggagttcga cgacggggcg gagcccgccg
     2821 aggagcagga accaggtgag caggtaggcg aaggcggcct ggacggaggc gggggtgagc
     2881 caggagatca ggaagacggc ggcgccggcg gcgaggacgg ttgcggcgcc gaaggggttg
     2941 cggatcagca ccagcatgcc gccgagcagc accaggctga tccacagcag cgcggtgacg
     3001 tggccggcgg cgagcagcca ggcgccgccg aggccgagca gcggcgaggc gatgtagccg
     3061 gcggcgctgg tgaggaccat cccggggccg gtcggcctgc ccgccgagac ggtcaccccg
     3121 gaggtgtcgg cgtgcaggcg gatgccgccg agccgccggc cggtgaccag ggcggcgagc
     3181 gcgtggccgc cctcgtgggt gatggtggcg acgttgcgcg cgacccgcca ggcgcggtcg
     3241 gggacgaccg ccgccagcgc cagcagcccg caggccagca ccagccaggt gggcgggtcg
     3301 ggctgggtgc cgaccacgcg gtgccagacc tcggtgatcg tgttgatgtc cgccctccct
     3361 cccgtcaagc ggcgcgcccg ttgcgtgagc gcgttccggt gtcgggttag cgtcgcatgg
     3421 aaagccggga ttctgcccgc tcctggctcg gctcggtagg ctcttggcat gactgactgg
     3481 gcaacgattt cgtcgctggc caccgctggg gccaccctgg cgcttgcggt cgccaccttc
     3541 tcgtcggtgc gctcggccaa ccgggcggcg cggaccgccg aacgggcgct ggaggcgcgg
     3601 ctgcgcccgc tgctgctgtc ctcccggctg gaggacccga cgcagaagat catctgggcc
     3661 gacgagcaca agaccacggt cggcggcggc agggccgccg tcgaggtggg cgctgacggc
     3721 accatctacc tggccatgtc ggtgcgcaac gtcggcgccg ggatggcggt gctgcacggc
     3781 tggtacccgc ggccggagtg gctgagcggc attccggcgc cgagcgacgt gcgccacgag
     3841 gaccccgagc ggttccggcg cctgacccgc gacctcttcg tccccgccgg ggacttcggc
     3901 ttctggcagg gcgccatccg cgacgccgac gatcccctgc gcgaggggct gcccgatgcc
     3961 atcgcggcgc ggcggcgttt cggcatcgac gtcctctacg gcgaccacga gggcggccag
     4021 cgcatcatca gccgcttcac gctcgctccg gctgccgaca gctcctggct gtgctcggtc
     4081 gcccggcact ggaacctcga ccggcccgac ccgcgctgaa tcagccgggg cgctcctccg
     4141 ggtcggcctc cggctgccag gcgcgcagca tgaagcgccg cagcttgccg gtcggggtgc
     4201 ggggcagcgc ctccaggaag gtcaccgccc ggggcacctt gaaggcggtc agctcggccc
     4261 ggcagcgggc gagcagctcc gcctccagcg ccgtccgggc cgcctcaccg ccgtgcgccg
     4321 gccccgcgac cacgaaggca cgcaggtggg tggccccatt cggatcgcgg acggcggcga
     4381 cggcgacctc ggtgacgccg tcgtgggtgg cgagcagccc ctcgatctcg gcgggggcga
     4441 ggttgatccc gccgacgttc tcgatgtcgt cggcgcggcc ggccatgcgc acgaacccgt
     4501 cgtcgtcgag caccgccagg tcgccgctgc gcagccactc gccgtggagc gccgcggcgg
     4561 tggcttccgg gttgttccag tacccgagca gcacggtcgg gccgttgacc cacaggacgc
     4621 cctgctcccc cggctccgcc ggcctcccac ggacagcatc gcttcgctcg cctgcgtgct
     4681 cggccggcgg gcacggcctg gccgcctccc cgccggcgtc gccgccggcg cggacctcga
     4741 cctggtaggg cggcagggcc cggccgacgg tgccgtcgcg ccaggcgtcg ggggtgttgg
     4801 acacgaaggt ctgccccacc tcggtggagc cgaggccgtc caggacgagg cagccgaagg
     4861 cagcacggac ccgttcggcc agggctggca gcaacgcctc gcccgccgag acggccaggc
     4921 gcaggtcgcg ggtgggcggc ctccctccgg caacggcggc gagcaggttg gcgtagaggg
     4981 tcggcacggc gaacaggatg gttggctggt ggcgctccag cagcgcgccg atcgcctcgg
     5041 gccggggccg cccggggtgc agcacggcgc tggcgcccga cagcagcggg aacgcgagcg
     5101 agttgcccag cccgtaggcg aagtacagct tggaggccga gaacagcacg tcgtcctcgc
     5161 ggacccgcag cgcccgccgc gcgaacgcct ggaagtagac ctccaggtcc cggtggcggt
     5221 gcagcgccgc cttgggccgc ccggtggtgc cggaggtgaa ctgcgcgtag gcgggcgtct
     5281 cgggggtgac cggcgcggcg ggggccggct ccacctggcg gattccagcc gccagccctc
     5341 cagccgaaag gaccggcacc ggagcgaagc gcccagccag ctcgtcgccg cagaccacgg
     5401 tcgagggcgt ggcgtcctcc agcatggcgg cgtggtcggc ggcggcgagc tgcgggttga
     5461 ccggcacggc gatggcgccg agccgggcgg cgccgaggaa cgcccaggcg aactcgaccc
     5521 cgtcgggcag ggcgatcagc acccggtcgc cagggccgac gccgtggcgt gccagcagcg
     5581 cggcggtgcg ggcggcgccg tcgtgcaccg cgccgtgggt gtagcgctcg tccccgacca
     5641 ggaaggcgct gcggtcgcgc cagccggagc gggcggccag cgtggcgagc cgctccgcga
     5701 ggttgtgccg gcgctccccc atctcgcgga gatgttaccg cctgttcctg cctgttactg
     5761 cttcgcgagg ctgtagagct gcgggccgag ccagtcccac accagggtgg cgccgtcggc
     5821 ggtgaagtgg acgccgtcat agcggacctt ggtcccgttg gccgggtcct gctggccgcc
     5881 ggggcagagg aagtcgcgca gggggagcac ggtgacccgg tcgcggtgcg cggcggcgaa
     5941 gcggccgagg acctggttca gccagacgcc cctggcgggg tcgtcgcggg ccttggcgat
     6001 ccaggcgtag gcgtcggcgg tctgcggctc ctggaagcag ggcacgttga ggatcgcgat
     6061 ccggcggctg accggtgcca gggtgtcgat ggcgagctgc agctcgctgt cgaggtagcg
     6121 ctcgtcgtcg gtgctgccgg cccgcagggt gcgcccgccg acccgcttgt cgagcacctc
     6181 ccaggcgccg atcagcagca ccgacacgtc cgggtggaag gcggccgcgt cggcgcgcca
     6241 gcgggtcggc caggtggtgc acttcgggtc gaccgcctgc accgcgccct cgtcctggtt
     6301 gacgctgggc gcgcgggcca ggccgcagcc aatgtcggtg tcgccgccca gcgtcatcct
     6361 cggcacggcc ccctgctggt agttgtagcc gagcgtgaag gccaccgagt cccccaccag
     6421 gaacacccgg aacccgttgc cgccgctgcc tggcgcttgc ccggcgcccg tgccgctgcc
     6481 cgggggcggc acggtgggga ggccggccgg cccggtaccc gcccgcaggg tggcgccctc
     6541 cgcgccagcg ccgggaccgg cggcggtggt gagcaccagc agcgctgcca gccccaccac
     6601 cgccccggcc gtcgccggcc gcgccaggcc gcggcggcgc agggcgccct ggcggatcgg
     6661 ctgctccagc aggaaatacg agaggagcgc cagcgcgaag gtggccgcca gccgcacggc
     6721 gagcagcggc accgggccca ggcgggtccg ctccggcgac agcgccacgt ccacaggcca
     6781 gtgccacagg tacagcccgt aggagatgcg cccgatcgcc ggcaggggtg ccagcgagag
     6841 cagctcccgg acccgcccgt cgccctccgg ctggaccgcc gcgacgatcg cgccggccgc
     6901 cgcgaccgcc gccagcgcga acccgccccg gtacagccag gtggcggtgt cgggcacccg
     6961 cagcatcagc gcggccagcc cgccgagccc ggcgaccccg aggctgtcgg cgagcacccg
     7021 cggcagcggc cggccgagcc gtgccagcgc gatcgccagg gcgccgccga gcagcagcgc
     7081 ctgcgcccgc gtgtcggtgc cgtagtagac ccgcgagggg tcggtgcccg gctggtacag
     7141 cagtgccatc agcagcgccg atccgagcgc gccgagcagc accagcccga gcagcggcgt
     7201 gggcagcccc cggcgggccc gccgcccggc cagcccgagc aggccgagca gcagcagcgg
     7261 ccacagcagg tagtactgct cctcgatgcc cagcgaccac atgtgggtga gcggcgacgg
     7321 gtcgccgaac tgcgcgaagt acggcaggtg gctgatggcg aaacgccagt tggcggcata
     7381 cccgagggtc gccagcccgt cccagcgcag ccgcgcccgg gtcgccgccg gcgccagcag
     7441 cgccccgtag gcggccaccg ccgccagcat caggaacagc gccggcagca gccgccgcgc
     7501 ccgccgggcc cagaagccct tcaggtcgat cccgccgcgc ccttcccact cggccagcag
     7561 cagcgtggtg atcaggtagc cagacagcac gaagaaaagg tcgacgccga ggaacccccc
     7621 gggcacccat cccacctgcc cgtggtagag cagcaccgcc gcgacggcga gcgcccgcag
     7681 cccgtcgagg gcgggctggt acggatagga acgctgcagc gccacaaagc cgcgagattc
     7741 caccgttctc ctcgtccttg tcaggggcac ggacgagcgg tcgccacaca ggctaccgga
     7801 cggtaacaaa ccctatcacg cggcacaagc ggttcaagcc ccggtctcct cccccagcca
     7861 ggcgaggtag tcggcgttgc cgtcgctgac cggcacggcg atcacctcgg ggacctcgta
     7921 ggcgtggtgc tccctgacgt aggcggcgag gcgctggaac cgtgcggcgg tggtcttggc
     7981 gaggcagagc cactcgtcgg cgcggtggcg ctcgccctgc caccagaaga cgctccagac
     8041 cgggccgacg acctgcaccg ccgcggccag gcgttcggtg acgatgccct ccgccagccg
     8101 cctggccgcg gcttcgtccg ggaccgcgac cgtcacctgt aaggggacga tgcctttgcc
     8161 cttgccttgt tcgaccatgt caccccacca gggttccggg gacggcaccg tcgagtgccg
     8221 ccagatcgtc ggcgtcgagg acgaggtcgg cggcgccgag ccaaccgtcg acctgggagg
     8281 ccttgcgggc gccgacgatc gcggcggtca cccccgtctg cgcggccacc caggcgaccg
     8341 cgaccacggc cacggagacg ccgtagcggg ccgcgatcgg ccgcagcgac tcggccacgg
     8401 ccaggttggc cgacagccgg ggctcctgga actgctcgtc gcggcggcgc cagtcgtcgc
     8461 gggcgagctc agccagccgc tgctcgctga acgcgccgga gagcaggcca gactgcatcg
     8521 ggctgtagca gatcgccccg gtgccgtgcg ccacacacca gggcagcagc acctcgagcg
     8581 cgctccggtc gatcagcgac agcggcggct gcagcgcgtc gacatggcgg atccgctcgc
     8641 agcgctccag caacgccacg tcgaagttag agacgccgag gcggcggacc ttgccggcgt
     8701 cggccagcgc cgccatggcg gcccacgacg cctcgatcgg cgtaccggtc tgccggtcgg
     8761 gccagtggat caggaacagg tcgatcgcct cggcgccgag gcggcgcagg ctctcgtcca
     8821 cctctcgccg gatcgtctgg ggccgcaggt cgtagaggtc gcggcgcccg tcggcccaga
     8881 gcccgcactt ggtggcgacg acgatccggt cgcggtcggc ccgctggcgc aggacccggc
     8941 cgaccagcag ctcggagtgg ccaaggccgt aggccggcgc ggtgtcgatc cagttcaccc
     9001 ccgcgtccag cgcgcggtgc agcgccgcga tcgactggcg gtcgtcctgg cggccccagc
     9061 cccaggccca cggcccgccg atcgcccagg cgccgaagcc gaccaccgac agtgccggac
     9121 cgtcccgccc gagagtccgg tagcgcatct cagtacccct ttcgctgaca gggagtcccg
     9181 gaaccgagtg tggggccggc ggcgctgtcg gccaggatca aatggaggtc gccgaagggg
     9241 ccccaggcgt agcggcggtc gagcgcggcg cggtaggcgc actccagcgg ctccagcccg
     9301 gcgagcgcct
//
LOCUS       DASWDC010000275         5195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1542325, whole genome shotgun sequence.
ACCESSION   DASWDC010000275 DASWDC010000000
VERSION     DASWDC010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542325"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="VF995_08870"
     CDS             complement(<1..1588)
                     /locus_tag="VF995_08870"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9343710.1"
                     /translation="MFAAIGRFTYRRRRLVAFVWLGIFVVGILVGPRVFAHLDSGNGL
                     RGNAESVVTLQRLNQATKSGTEVTALLDGRDVHDPALRASVAAAVADLRARPDVRRVI
                     DPVATPVPALLASDRRAMLLEVELRDDLSVSQENQASDQVTARLRRISAPRVLVGGNR
                     AANLDFQDRAQKDLERGEMIALPFVVVLLFLIFRGVVALLVPLVVALVAIAGALLVLL
                     GVSQLTDTSTYSVNVITMLGIGLAVDYSLLIVSRFREERAGHEAAAAIERTLATAGRT
                     VAFSGLTVAIALSGLLVFAVPFLRSMAWGGIGVVLVAMVAAITLIPALLGLWGRRIKP
                     ARATRSDHGVFYRVSRVVQRFAWALVPLLVVGLALLAVPFAHAKLENSGVEALPRSSG
                     VRQLFETLRDRFPGRGSDPIIVLAEINPADPQAQAFVARVRALPGVASVAPHQGVPPV
                     VTVLDVTPDGPSQGAVATRLVSQIRGLERPVAVYVTGSVVVPLKAVVMNVLSLGASFG
                     ALVWVFQEGHLSGLLGFEPTG"
     gene            1856..3892
                     /gene="ligA"
                     /locus_tag="VF995_08875"
     CDS             1856..3892
                     /gene="ligA"
                     /locus_tag="VF995_08875"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX9343711.1"
                     /translation="MATPVPADEAARRVDELHAQLRDASYRYYVLSDPDISDAEYDEL
                     LRELTRLEQEYPDLVTPDSPTQTVGAPPSAAFAPVRHRVPMLSLDNVFSFEELAAWGR
                     RTEKAVAEVGAYVCELKIDGTAVSLQYERGRLVRAATRGNGTVGDDITPNVRTIQNVP
                     ERLALDDPPPVFEVRGEIFYPTRAFEKLNDQMAVEGRQVFANPRNAASGALRQKDPRV
                     TASRPLAMLCHSFGQADGMRFASHSEFLAFCEAAGLPVAAETTRVATLDEVEAFVRSW
                     SEHRHDLDHEIDGVVVKVDSTNQQQELGRTSRFPRWAIAFKFPPEERTTQLLDIEISI
                     GRTGAATPFAVLEPVLVAGSTIGLATLHNQDEVARRDVRPGDTVFVRKAGDVIPEVVG
                     PVLNKRPADSQPWEFPRECPECGTPLVRPEGESVTRCPNTTGCPAQRWATLVHFGSRG
                     AMDIEHLGERTVAALIDAGNVRDVADIYQLTADDLAALAGFKDKSIANLLAAIEASKQ
                     RPLDRLLVGLSIRHVGDHVATVLANRLKSLEAIAAASEEQLAAVDEIGPTIAASVAAW
                     FADEGNQRLIRRLAEAGVATEADGGDEPALPQTLAGKALVLTGGLEGYTRDEASSAIE
                     ERGGRVTSTVSKKTDYVVVGADPGSKAARATELGVPTLDEAAFRDLLERGEIEP"
     gene            3889..4470
                     /locus_tag="VF995_08880"
     CDS             3889..4470
                     /locus_tag="VF995_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343712.1"
                     /translation="MSQVWTPWDPEGSRPQQPSGQERGPDPEPIVRTVRIGFLLAGIM
                     VAAMINAGLHQTLARVPDDSGVALTGVLAGGVLAVVGWAVWAFRPSRLLLIGKRAAQS
                     PNPRERRPRAERARAMGFRGLAMSWTGQAMMFCFVPALAGLALDMVHGQAWELLAFSA
                     ASLLAGFLLQHQVAGAVRLAVDDPELQARYGSR"
     gene            complement(4516..>5195)
                     /locus_tag="VF995_08885"
     CDS             complement(4516..>5195)
                     /locus_tag="VF995_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX9343713.1"
                     /translation="TWIECHHCETRLTLPPAGPGRVTTGEAALQPGAPPAGRERRERV
                     VRLLAVVVLLAVVAVVVMVATRGLRHPHAAVAAKPAPGTAVASLVVVRPLTATASSES
                     GERVARNVIDRNLTTYWSRHVPGTDAQPFLQFTFATPVKLTELQIAAGASGDQFIARP
                     RPQRIALLFADGSSLNFSLADKRGFQKISFPPRSVGKFRLVILSIYPGTDLPRTSISE
                     VKFLAAP"
BASE COUNT          775 a         1877 c         1859 g          684 t
ORIGIN      
        1 tgccggtcgg ctcgaagccg agcaggcccg acaggtggcc ctcctggaac acccagacaa
       61 gcgcgccgaa gctggcgccg agcgacaaca cgttcatcac caccgccttc agcggcacca
      121 ccaccgaccc ggtcacatac accgccaccg gtcgctccag gccgcggatc tggctcacca
      181 gccgtgtggc caccgctccc tggctgggcc cgtccggggt cacgtccagc acggtgacaa
      241 ccggcggcac gccctggtgg ggtgcgaccg aggccacgcc gggaagcgcg cggacgcggg
      301 cgacgaaggc ctgggcctgg gggtcggcgg ggttgatctc ggcgaggacg atgatcgggt
      361 cgctgccgcg cccggggaag cggtcccgca gcgtctcgaa cagctgccgc acgccggagg
      421 atcgcggcag cgcctccacc ccggagttct ccagcttcgc gtgggcgaac ggcaccgcca
      481 gcagcgccag cccgaccacc agcagcggga ccagcgccca ggcgaagcgc tgcaccaccc
      541 gcgagacgcg gtagaacacg ccgtggtcgg agcgggtcgc tcgcgccggc ttgatccggc
      601 gcccccacag gcccagcagg gcagggatca gggtgatggc ggcgaccatc gcgaccagca
      661 ccacgccgat gccaccccag gccatcgagc gcaggaatgg caccgcgaac accagcagcc
      721 cggacagggc gatcgccacg gtgagcccgg agaaggccac cgtccgcccg gcggtggcca
      781 gcgtccgctc gatcgcggcg gcagcctcat gtccggcgcg ctcctcccgg aaccggctca
      841 cgatcagcag cgagtagtcc accgccaggc cgatgccgag catggtgatg acgttgaccg
      901 agtaggtgga ggtatcggtc aactggctga ccccgagcag caccagcagc gccccggcga
      961 tcgccaccag cgcgaccacc agcggcacca gcagcgccac gacccctcgg aagatgagga
     1021 acagcagcac caccacgaac ggcagcgcga tcatctcgcc gcgttccagg tccttctggg
     1081 cgcggtcctg gaagtcaagg ttcgcggccc ggttgccgcc gaccagcacc cgtggcgcgc
     1141 tgatccgccg caaccgcgcg gtgacctggt cgctcgcctg gttctcctgg ctgacggaga
     1201 ggtcgtcgcg cagctccacc tccagcagca tggcgcgccg gtcgctggcg agcagggcgg
     1261 ggaccggcgt ggccaccggg tcgatcacgc ggcggacgtc gggccgggcg cgcaggtcgg
     1321 cgacggcggc ggcgacgctc gcccgcaggg cggggtcgtg gacgtcgcgg ccgtcgagca
     1381 gcgcggtcac ctcggtgccg ctcttggtgg cctggttgag gcgctgcagc gtgaccacag
     1441 actcggcgtt gccgcgcagg ccgttgccgc tgtcgagatg ggcgaagacg cgcgggccaa
     1501 ccaggatgcc gaccacgaaa atgccgagcc agacgaatgc gacgagccgc cgccgccggt
     1561 aggtgaaccg gccgatggca gcgaacattg cgaccctccc gtgcctggct gccctgcctg
     1621 gtggtggtgc tggctgctgc ctgctggccg ttggtggttg gctcaccgag ggccattcgc
     1681 gcagactgtg acgtactact ctacgtcggc aggtccagac atacatctgc tttgcttcgg
     1741 aggccgccgt tctcgcccgg tggcctgtgg atatccggga agtcctgtcc ctggtcctgg
     1801 taatactggg agtgaccttc gaccacagca ccgaaatgcc cagggggaag cgcacgtggc
     1861 gacaccagta ccggcggacg aggcggccag gcgcgtcgac gagctgcatg cccagctccg
     1921 ggacgcctcc taccggtact acgtgctcag cgatccggac atctccgacg ccgagtacga
     1981 cgagctgctg cgtgagctga cccggctgga gcaggaatac cccgacctgg tcacccccga
     2041 ctcgccgacc cagacggtcg gggcgccgcc gtcggcggcg ttcgcgccgg tgcggcaccg
     2101 ggtgccgatg ctctcgctgg acaacgtctt ctcgttcgag gagctggccg cctggggcag
     2161 gcgcaccgag aaggcggtcg ccgaggtcgg cgcctacgtc tgcgagctga agatcgacgg
     2221 cacggcggtg tcgctgcagt acgagcgggg ccggctggtc cgcgcggcga cccgcggcaa
     2281 cggcaccgtc ggtgacgaca tcaccccgaa cgtgcgcacc atccagaatg tcccggaacg
     2341 gctcgccctc gacgacccgc cgcccgtctt cgaggtccgc ggcgagatct tctacccaac
     2401 ccgggcgttc gagaagctca acgaccagat ggccgtcgag ggcaggcagg tcttcgccaa
     2461 cccgcgcaac gccgcctcgg gggcgctgcg gcagaaggac ccgcgggtga ccgcgagccg
     2521 gccgctggcg atgctgtgcc actccttcgg gcaggccgac gggatgcgct tcgcctccca
     2581 ctcggagttc ctcgccttct gcgaggctgc cgggctgccg gtcgccgccg agaccacccg
     2641 cgtcgccacc ctggacgagg tggaggcctt cgtccgctcc tggagcgagc accgccacga
     2701 cctggaccac gagatcgacg gcgtggtggt caaggtcgac tccaccaacc agcagcagga
     2761 gctgggccgc acctccaggt tcccgcgctg ggcgatcgcc ttcaagttcc cacccgagga
     2821 gcgcaccacc cagctcctgg acatcgagat ctccatcggc cgcaccggcg cggcaacccc
     2881 gttcgcggtg ctggagccgg tgctggtggc cggctccacg atcggactgg cgaccctcca
     2941 caaccaggac gaggtcgccc gccgcgacgt gcgccccggc gacaccgtct tcgtccgcaa
     3001 ggcgggcgac gtgatccccg aggtggtcgg cccggtgctg aacaagcgcc cggcggactc
     3061 ccagccctgg gagttcccgc gcgaatgccc tgagtgcggg accccgctgg tccggcctga
     3121 gggcgagagc gtgacccgct gccccaacac caccggctgc ccggcgcagc gctgggccac
     3181 cctggtccac ttcggctcgc gcggggccat ggacatcgag cacctggggg agcggaccgt
     3241 cgccgccctg atcgacgccg gcaacgtgcg cgacgtcgcc gacatctacc agctcaccgc
     3301 cgacgacctg gccgcgctgg ccggcttcaa ggacaagagc atcgccaacc tgctcgccgc
     3361 catcgaggcg tccaagcagc gcccgctcga ccggctgctg gtcggcctgt ccatccgcca
     3421 cgtcggcgac cacgtcgcga cggtgctcgc caaccggctg aagtcgctgg aggcgatcgc
     3481 cgccgcctcg gaggagcagc tcgccgcggt cgacgagatc ggcccgacga tcgcggccag
     3541 cgtcgccgcc tggttcgccg acgagggcaa ccagaggctg atccgccgct tggccgaggc
     3601 gggcgtcgcc accgaggccg acggcgggga cgagccggcg ctgccgcaga ccctggccgg
     3661 gaaggcgttg gtgctgaccg gcggcttgga ggggtacaca agggacgagg cgagctcggc
     3721 gatcgaggag cggggcggca gggtcacctc gactgtctcc aagaagactg actacgtggt
     3781 ggtcggcgcc gatcctggct ccaaggccgc ccgcgccacc gagctgggcg tcccgacgct
     3841 tgacgaggcg gcctttcgcg atctgctgga acgaggtgaa atagaaccgt gagccaagtt
     3901 tggactccct gggacccgga agggtcgcgg ccgcagcagc cgtcagggca ggaacgcggg
     3961 cccgacccgg agccgatcgt ccgcacggtg cggatcggct tcctgctggc cggcatcatg
     4021 gtcgcggcga tgatcaacgc cggcctgcac cagaccctcg cccgcgtccc cgacgattcg
     4081 ggtgtggccc tgaccggggt gctggctggc ggggtgctgg ccgtggtcgg ctgggcggtg
     4141 tgggcgttcc ggccctcgcg gctgctgctg atcggcaagc gcgccgcgca gtcccccaac
     4201 ccgcgtgagc gccgcccccg cgccgagcgt gcccgcgcca tgggcttccg cggcctggcg
     4261 atgtcgtgga ccggccaggc gatgatgttc tgcttcgtcc ccgccctcgc cggcctcgcc
     4321 ctcgacatgg tccacgggca ggcctgggag ctgctcgcct tctccgccgc cagcctgctc
     4381 gccgggttcc tgttacagca ccaggtcgcc ggcgcggtcc gtctcgccgt cgacgacccc
     4441 gagctgcagg ctcgctacgg gtcgcgctga ccggccaccg acggctgcgc ggcgccgagg
     4501 cgtccggcca cgcggctacg gggccgccag gaacttgacc tcgctgatgg aggtccgggg
     4561 gaggtcggtg cccgggtaga tgctgaggat caccaggcgg aacttgccga cgctgcgcgg
     4621 cgggaagctg atcttctgga aaccgcgctt gtcggccaag ctgaagttca ggctggagcc
     4681 gtcggcgaac agcagcgcga tgcgctgggg ccgcggccgg gcgatgaact ggtcgccaga
     4741 ggccccggcg gcgatctgca gctcggtgag cttcaccggc gtcgcgaagg tgaactgcag
     4801 gaacggttgt gcgtcggtgc cggggacgtg ccgtgaccag taggttgtca ggttgcggtc
     4861 gatcacgttg cgggcaactc gctccccgga ctcgctggag gcggtcgcgg tcagcgggcg
     4921 gaccaccacc agggaggcga cggcggtgcc cggtgccggc ttggcggcga ccgcggcgtg
     4981 cgggtggcgc agcccccggg tcgccaccat cacaaccacg gcgacgactg cgagcagcac
     5041 gaccacggcc agcaggcgca cgacccgctc ccgccgctcc cgcccggccg gcggtgcgcc
     5101 aggctggagg gcggcctcgc ccgtggtgac ccgcccgggc ccagctggcg gcagggtcag
     5161 gcgcgtctcg cagtggtggc actcgatcca tgtgg
//
LOCUS       DASWDC010000276         5884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1495694, whole genome shotgun sequence.
ACCESSION   DASWDC010000276 DASWDC010000000
VERSION     DASWDC010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495694"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /gene="tatC"
                     /locus_tag="VF995_08890"
     CDS             complement(<1..631)
                     /gene="tatC"
                     /locus_tag="VF995_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX9343714.1"
                     /translation="MTILEHLAELRDRLIKSCAALVITSLVAFFVLYNPALKLLTTSY
                     CHLPAKYRFANAAGVARCQLLATSPLDPFGIRLRVSAVLGLALAMPIVAFQIWRFITP
                     GLKRRERRYAIPFSLATTLLFVFGLGMAFFTLPKAIGFLVGIGGTNLLSLFTADEYLR
                     FVFFMGLAFGITFEFPLVLVALSMAGVLSSRAMIKAWRPAVVIIIVVAAV"
     gene            complement(709..960)
                     /locus_tag="VF995_08895"
     CDS             complement(709..960)
                     /locus_tag="VF995_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX9343715.1"
                     /translation="MFEDKWIFVILIIALLFGASRLPMLGRNFGAGIKEFKKGLAESQ
                     RDDKSGAKAEQADKAEAAIDQPEAARPASGSSDENGAGA"
     gene            complement(1052..1330)
                     /locus_tag="VF995_08900"
     CDS             complement(1052..1330)
                     /locus_tag="VF995_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343716.1"
                     /translation="MEVPLPTWLAILLGVFWLGVLCSVVFTALRLWGNLKGLLTSVNN
                     LTEQLAPALEDLNAQAQDAAEVAARLQRSQAAVKRNQRRRAAGRERHR"
     gene            complement(1375..1776)
                     /locus_tag="VF995_08905"
     CDS             complement(1375..1776)
                     /locus_tag="VF995_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343717.1"
                     /translation="MDAYRNDDLDDREPETRIRATCPDCGEVELTPPQVELRLGRLGR
                     RGLYRFTCPSCTLLVTKAADRRIVRLLASAGVKATLPSAGPGTGADREQLPAEALEPH
                     HGPPLTWDDLLDFHFELQAPGWWARFLAACR"
     gene            complement(1838..2893)
                     /locus_tag="VF995_08910"
     CDS             complement(1838..2893)
                     /locus_tag="VF995_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX9343718.1"
                     /translation="MSTTPGTGSGVGAGPGRRGAADTSAGARLRRVLALVPWLMAHPE
                     ATVDEICARFTMTREQLIADLDLLYVSGVPPYGPGDLMEAWIDGDKVHIALADYFARA
                     PRLTWREAAGLYLAGRALAALPEIAEQGSLTRALAKLEAVLPADQLDRVQELAGKVSV
                     DLDSGEGGAERDHRAALVRAAAADRRVLLEYWSGSRGDLTRRKVDPWLVFAASGHWYM
                     AGYCHKAAGERLFRVDRIVSVTQTGEPFERPARFDPAAYAESPWSGAFDQGVECELDL
                     DKRAEWVAEYFPILAQERRPDGRLRVRLATNELAWVVRLILRLAPDAEPVSPSELRQA
                     VAEAAHHNRAPYRDASR"
     gene            complement(2890..3867)
                     /locus_tag="VF995_08915"
     CDS             complement(2890..3867)
                     /locus_tag="VF995_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX9343719.1"
                     /translation="MRKVERLVNLTALLLDTHRPLTLDQIAELVPGYEATGESLRRMF
                     ERDKEELRGLGVPVERTPIDAWGTDEGYYIDPGAYAMPALDLAPDERAALALAARAWS
                     GGGAGDAAAVAGLAKLDLDPGAGPDTVQANLDGTSPLLAALLEAVTSRKRIGFSYQPP
                     GREPTERRLDPYALIHRRGTWYVAGHDADREALRSFRLSRFRGAVRLAKPTSRGPEYE
                     VPVDFDPRELLPTAGEPDAMPIAIVRADESAARLAELRGAKREGDPDASGRVTLRLPV
                     GDPSTFLMWAIGNAVEVVEPPELRAEAASRLGGLLDLVDPGQRRQVNGG"
     gene            3998..4672
                     /locus_tag="VF995_08920"
     CDS             3998..4672
                     /locus_tag="VF995_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9343720.1"
                     /translation="MTEPAPSPSTRRAVLDLLKREGPQTVARLGEALGVSGVAVRRHL
                     EALISDGLVTQTASASGRGRPAHVYALTEAGHDQFPRNYHQLAAQLLEAVEERFGAGA
                     LESLFERRQQVLAALYAARTHGRPLGALAAELAAIQDENGYMADWEQTGTDRYVVSEH
                     NCAIRCVASAYPQACQHELALFQQLAGPAVEVQRIEHLQAGDSRCAYVLRPASTPTPN
                     ANGAGE"
     gene            4702..5832
                     /locus_tag="VF995_08925"
     CDS             4702..5832
                     /locus_tag="VF995_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018811728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HEX9343721.1"
                     /translation="MIRIRNGTVTALLARRPGATEVAVSVEPEGVEARALGYDDLTGP
                     LKVGDRVVLNTTAVALGLGTGGLHLVMARLDGPGDRHDGHDEGGPGSPGHLMKARYTP
                     AQVRVLAVEEEASPHRGAVEAVTDLGGMPVVCAELHSMVPVIAAAARAAGADLTVAYV
                     MSDGAALPLAYSRLAAELRDAGLVAGIVTAGQAFGGDLEAITLHSGLAAARAVLGADV
                     AVVAQGPGGAGTGTAFGYSGTQVAEMLHAAAALGGRPIACLRMSTADRRERHRGVSHH
                     SLTVLGRLTLTRVTVALPALDDPALAALVNRQLVAAGVAGRHELIAVHPPADPAALLA
                     SWNLKVTTMGRGPDDDPVFFAAAAAAGTLAAQLVQVRRGAGG"
BASE COUNT          785 a         2089 c         2230 g          780 t
ORIGIN      
        1 tcaccgccgc gacgacgatg atgatcacca ccgccggccg ccacgccttg atcatggcgc
       61 gcgatgacag cacccccgcc atcgacagcg ccaccagcac cagcgggaac tcgaaggtga
      121 tcccgaaggc gagccccatg aagaacacga agcggagata ttcgtcggcg gtgaacaggc
      181 tgagcaggtt ggtgccgccg atcccgacca ggaagccgat cgccttcggc agggtgaaga
      241 aggccatccc caggccgaac acgaacagca gcgtggtcgc cagcgagaat gggatcgcgt
      301 agcgcctctc cctgcgcttg agccccgggg tgatgaaccg ccagatctga aaggcgacga
      361 tcggcatcgc cagcgccagg ccgagcaccg ccgagacccg cagacggatc ccgaacgggt
      421 ccagcgggct ggtcgccagc agttggcagc gcgccacccc cgccgcgttg gcgaagcggt
      481 acttggcggg cagatggcag taggaggtgg tgagcagctt cagcgccggg ttgtagagca
      541 cgaagaacgc gaccaggctg gtgatcacca gcgcggcgca gctcttgatc agccggtcgc
      601 gcagctcggc gagatgctcg aggatcgtca tctcgccctc gggattcacc cgagaacgac
      661 gctggaggcg catggcggag gcgcgagcgc tctgtggggc gggggcgcct acgcgccggc
      721 gccgttctcg tcactggagc cggaagccgg cctggccgcc tcgggctggt caatggccgc
      781 ctcggccttg tcagcctgct cggccttcgc cccggacttg tcgtcgcgtt gggattcggc
      841 gaggcccttc ttgaactcct tgatgccggc cccgaagttg cggccgagca tcgggaggcg
      901 gctcgcgccg aacagcagcg cgatgatcag gatcacgaag atccatttat cttcaaacat
      961 cttcctgctc ccttttgtct gcccccgagc cgacggctcg gttgagtcta ccgctccgcg
     1021 gcgtttccgg gcagtcgggc aatcctgccg ctcagcggtg ccgctcgcgg cccgccgccc
     1081 gccgccgctg gttgcgcttc acggccgcct ggctgcgctg caggcgcgcg gccacctcgg
     1141 cggcgtcctg cgcctgcgcg ttgaggtcct ccagcgccgg ggcgagctgc tctgtgaggt
     1201 tgttcaccga cgtgagcagc cccttcaggt tgccccacag ccgcagcgcg gtgaacacga
     1261 cgctgcagag cacgccgagc cagaacacac cgagcaggat ggccagccag gtcggcagcg
     1321 gaacctccac gggtcggggc cagggccagg gcggtgccat gacgcccgct catcctacct
     1381 gcaggccgcg aggaagcgcg cccaccagcc gggcgcctgc agctcgaagt ggaagtcgag
     1441 caggtcgtcc caggtcagcg ggggtccgtg gtgcggttcg agcgcctcgg cggggagttg
     1501 ctcccggtcg gcgccggtcc cgggaccggc gctggggagg gtggccttga ccccggccga
     1561 ggcgagcagg cggacgatcc ggcggtcggc ggccttggtg accagcaggg tgcagctcgg
     1621 gcaggtgaac cggtagaggc cgcggcgccc gagccggccg aggcgcagct cgacctgcgg
     1681 cggggtcagc tccacctcgc cacagtcagg gcaggtcgcg cggatccggg tctccggctc
     1741 gcggtcgtcg aggtcgtcgt tgcggtaggc atccacggac gcgggtatcg accctcgcct
     1801 gggggacctt gaggagaacc ggtgccgccc cggcgggtca gcggctggcg tcgcggtagg
     1861 gggcgcggtt gtggtgggcg gcctcggcga ccgcttggcg cagctcggag gggctgaccg
     1921 gttcggcgtc gggagcgagg cgcaggatca ggcggacgac ccaggccagc tcgttggtgg
     1981 ccaggcggac ccggaggcgg ccgtcggggc ggcgttcctg cgccaggatc ggaaagtact
     2041 cggcgaccca ttcggcgcgt ttgtccaggt cgagctcgca ttcgacgccc tggtcgaacg
     2101 cgccgctcca gggcgactcg gcgtaggcgg ccgggtcgaa gcgggcgggt cgctcgaacg
     2161 gctcgccagt ctgggtgact gagacgatcc ggtcgacgcg gaacagccgc tccccggcgg
     2221 ctttgtggca atagcccgcc atgtaccagt gcccggaggc ggcgaacacc agccagggat
     2281 cgaccttgcg gcgggtcagg tcgccgcggg agccgctcca gtattcgagc agcacccgcc
     2341 ggtcggcggc ggcggcacgg accagcgcgg cccggtggtc ccgctcggcc ccgccctcgc
     2401 cgctgtcgag gtcgaccgag accttgccgg ccagctcctg gacccggtcg agctggtcgg
     2461 cgggcaggac cgcttcgagc ttggcgagcg cgcgggtgag cgagccctgc tcggcgatct
     2521 ccggcagggc agcgagcgcc cgcccggcga ggtacagccc ggcggcctcc cgccaggtga
     2581 ggcgcggcgc gcgggcgaaa tagtcggcca gggcgatgtg gaccttgtcg ccgtcgatcc
     2641 acgcctccat caggtcgccc ggcccatacg gcggcacccc ggagacgtag agcaggtcca
     2701 ggtcggcgat cagctgctcg cgggtcatgg tgaagcgcgc gcagatctcg tcgacggtgg
     2761 cctccggatg cgccatcagc cacggcacca gggcgagcac ccgccgcagc cgggcgcccg
     2821 cgctggtgtc ggcagcgcct cgccgtccag gcccggcgcc cacgccggag ccggtccccg
     2881 gagtggtgct catcccccgt tcacctgcct ccgctgtccc ggatcgacca ggtcgaggag
     2941 cccgccgaga cggctggccg cctcggcgcg cagctccggc ggctcgacaa cctcgacggc
     3001 gttgccgatc gcccacatca ggaaggtcga ggggtcgccg actggcaggc gcagggtgac
     3061 ccggccgctc gcgtcggggt cgccctcccg cttggcgccg cgcagctcgg cgagacgagc
     3121 ggcgctctcg tcggcccgca cgatcgcgat cggcatggca tcgggctcgc cggccgtcgg
     3181 cagcagctcc cgcgggtcga agtccaccgg cacctcgtat tccggcccgc ggctggtcgg
     3241 cttggccagc cgcaccgccc cgcggaaccg ggagagccgg aaggagcgca gcgcctcgcg
     3301 gtcggcgtca tggccggcca cataccaggt gccgcgccgg tggatgagcg cataggggtc
     3361 gaggcgacgc tcggtcggct cgcggcccgg cggctgatag gagaacccga tccgcttgcg
     3421 gctggtcacc gcctccaaga gggccgcgag cagcgggctg gtcccgtcga ggttggcctg
     3481 gaccgtgtcc gggccagcgc cggggtccag gtcgagcttg gccagcccag cgaccgcggc
     3541 ggcgtcgccg gcgccgccgc ccgaccaggc ccgcgcggcc agcgccagcg cggcgcgctc
     3601 gtcgggggcg aggtcgaggg ctggcatggc gtaggcaccc gggtcgatgt agtagccctc
     3661 gtcggtgccc caggcgtcga tcggggtacg ctcgaccggc actccgagcc cgcggagctc
     3721 ctctttgtcc cgctcgaaca tacgccgcag actctccccg gtggcctcgt agccgggcac
     3781 cagctcggcg atctggtcga gggtgagcgg gcggtgggtg tcgagcagga gggcggtcag
     3841 gttgacgagc cgctcgacct tgcgcatgcg cgcatgctag tcgttttggg ctgctctggt
     3901 cgagcggttt cccccggccg cgttgccgtc agcggatggg gccgggtagc ctggcgggcg
     3961 tgacgtgaac agtgccgtcc agttcatcgg agcgtgcgtg accgagccag ctcccagccc
     4021 ctcgacccgg cgcgcggtgc tcgacctgct caagcgcgaa gggccccaga ccgtggcgcg
     4081 cctcggcgag gcgctcggcg tctccggggt ggcggtccgc cgccacctgg aggccctgat
     4141 cagcgacggc ctggtgacgc agaccgccag cgcgagcggc cggggccgcc ccgcccacgt
     4201 gtacgcgctc accgaggctg gccacgacca gttcccccgc aactaccacc agctcgccgc
     4261 ccagctgctg gaggcggtcg aggagcgctt cggcgcgggc gcgctggagt cgctgttcga
     4321 gcgccgccag caggtcctcg ctgcgctgta cgcggcccgc acccatggcc ggccgctcgg
     4381 cgcgctggcg gcggagctgg ccgcgatcca ggacgagaac ggctatatgg ccgattggga
     4441 gcagaccggc accgaccgct atgtggtcag cgagcacaac tgcgccatcc gctgcgtggc
     4501 cagcgcctac ccgcaggcct gccagcatga gctggcgctg ttccagcagc tcgccggccc
     4561 ggcggtcgag gtgcagcgga tcgagcacct gcaggccggc gactcccgct gcgcctatgt
     4621 gctgcgtccg gcgtcgaccc ccacccccaa cgccaacggc gccggcgagt agccgcggcg
     4681 ccggcgcggg ataatcccgg gatgatcagg atccgcaacg gcacggtgac cgcgctgctg
     4741 gctcgccggc ccggcgccac cgaggtggcc gtctccgtcg agcccgaggg ggtcgaggcg
     4801 cgcgccctcg gctatgatga cctgaccggg ccgctcaagg tcggcgaccg ggtggtgctg
     4861 aacaccaccg cggtcgccct gggacttggc accggcggcc tccacctggt gatggctcgc
     4921 ctcgacggcc ccggtgaccg ccatgacggc catgacgaag gcggtccggg aagcccaggg
     4981 cacctgatga aggcccgcta cacccccgcg caggtgcggg tgctggcggt cgaggaggag
     5041 gcgagcccgc accgcggcgc cgtcgaggcg gtcaccgatc tgggtgggat gccggtggtc
     5101 tgcgcggagc tgcattccat ggtcccggtg atcgcggcgg ccgcccgcgc cgccggtgcg
     5161 gacctcaccg tcgcctatgt gatgagcgac ggggccgcgc tgccgctggc gtactcgcgg
     5221 ctggccgccg agctgcgcga cgccgggctg gtcgccggga tcgtcaccgc cgggcaggcg
     5281 ttcggcgggg acctggaggc gatcaccctc cactcggggc tggcggcggc gcgggcggtg
     5341 ctcggcgccg atgtggcggt ggtggcgcag ggcccggggg gggctgggac cgggacggcc
     5401 ttcgggtact ccggcacgca ggtggcggag atgctgcacg cggcggcggc gctcggcggg
     5461 cggccgatcg cctgcctgcg gatgtccacc gccgaccggc gggagcggca ccggggcgtg
     5521 agccaccaca gcctgaccgt cctcggccgc ctcaccctca cccgggtcac cgtggcgctg
     5581 ccggcgctcg acgacccggc gctcgccgcg ctggtcaacc ggcagctcgt cgcggcgggt
     5641 gtggccgggc ggcacgagct gatcgcggtg caccctccag cggatccggc ggcgctgctg
     5701 gcgagctgga acctcaaggt cacgaccatg ggccgcgggc cggacgacga cccggtgttc
     5761 ttcgcggcgg cggccgccgc cgggaccctc gccgcccagc tcgtgcaggt gcggcggggg
     5821 gctggcggat gagcgaagcg gcgccggcgc cggcggcggc acgggctggg ggacgcaccc
     5881 gagg
//
LOCUS       DASWDC010000277         7110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_374825, whole genome shotgun sequence.
ACCESSION   DASWDC010000277 DASWDC010000000
VERSION     DASWDC010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374825"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VF995_08930"
     CDS             <1..494
                     /locus_tag="VF995_08930"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX9343722.1"
                     /translation="PELGFCLTTRGQMFWLTEGLPNSLEPGKRPRTTLSPSLALRDGQ
                     PWLACGTPGGDQQDQWSLQLLLNHLHFGMDLQEAIDAPAFHSDHFPSSFYPREGRPGV
                     VVIERRAGGAVIEALRARGHQVEVADPWSLGRLCAVGRDPETGLLMAAANPRGRAGYA
                     VGR"
     gene            complement(525..1709)
                     /locus_tag="VF995_08935"
     CDS             complement(525..1709)
                     /locus_tag="VF995_08935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9343723.1"
                     /translation="MQPQPLLEARDWLKSQNPVQLTRAINVPAIRQRVSAVLVTYNQC
                     PFAANSGRLADNPLTWALDSLLAQTGEALREIVVVDDGSTDHTPMIVEAYRYMIASTE
                     RTTRVVTLRHKRRRGLGAGRNTGIAQATGDLVLFGDDDAVFRPHCVTGAAYTLTLLRD
                     HDPRACALALPSYDRSLWPQQIADLAYIGRLEPEAARFSARFDCYPAEYLRDPQLLDN
                     ESALLAPFPVELMNGTCLVDAATLRQAGGFPDLSSWPAASPEHLYLAVALSRNGGHLY
                     HMPDPRLAARTDASARGQTPSSLADLIGSYFAFFVAASQRGGAAWGIRSHQEFVVTGA
                     SEMLRADQMPPNERRRQWRDGLRRGTRFAADNPPRVSPMDLQNALDEVTRTVREPRIA
                     LW"
     gene            complement(1918..3447)
                     /locus_tag="VF995_08940"
     CDS             complement(1918..3447)
                     /locus_tag="VF995_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343724.1"
                     /translation="MRSDTSRALYKRIDVLRRDWEAAGLAPGAIVCEILAASPEISPL
                     QAHRLARGLTLAQVVRGAGQLYSDDGLMPPPGLNTSRLHEWEHWPRSGRRPGEEARDY
                     LARFYETRQDKLGFGHDYSLDEGDSPGSEDPQGGHVEIHTSAGGRTVHVELPGGNHPT
                     IEVDEEDGLKRRVVVMLGGLVVAEKLLPAVDPERLAWARANPSRVDLELLRDLRATTM
                     AYAGELASSPTVRLAEPARAHLDYLVGLLHGSQPESIERRLRVITGEAAVLAGWTTFQ
                     CEDRDAARADYALARLLARQTGDQQLLAETFIPMSYLYSTRYAAAGRQTNPTKALTLL
                     RAAGAGPGGARLAAPMRGWAAGCRAAEHAFRGEADAARRELDRAEDALAEIRPGEEHG
                     FLAYWEQTRVDGYVGLCNLLLGRGEAAEQALTKSLETADLSLHRHRLGVKVDLGIARV
                     LQGEIEAACATLEEACGHAERGKFPIVLRRIRNVHREHLARWHDLRVVRSLDEHLHES
                     A"
     gene            3585..4796
                     /locus_tag="VF995_08945"
     CDS             3585..4796
                     /locus_tag="VF995_08945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9343725.1"
                     /translation="MRILLIGPDHDRGSIPPYLDLLAAGLGERGAQVTRLSAGGLPLD
                     RESARESARESAAPRFWSAERIVAAAEALVARIDPDAFDLVSLHYGNLEVEQLVPVLW
                     ERRFGPSGSGSGPPIVAHAHFVDWTLFSTHVVDSALYQAVRTGIGRMDGLVFFGEYAR
                     RALLDRDGLRGVPTLVSYFPTTIPAGERAPGPGALRPALRELDPASRAGRGQGGGLLP
                     LATMYGFANPWKDPSDLLAAFQRMRVPLMVLLAGHGWDRPERAGADLRAALSPRRLRI
                     GPVELTVLADYIGPGERLALVAASDFAVFPYRRHESFQGSGAITDYLANGVPVVATDV
                     ANMAELIGDAGVVVPPGDPAALAATLDRVAGDAGLRAALALAARRRAPRFSLARHLDD
                     CDAFYRTLAAR"
     gene            4935..5093
                     /locus_tag="VF995_08950"
     CDS             4935..5093
                     /locus_tag="VF995_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343726.1"
                     /translation="MMESIVAATLLLAVVLVAYLADVLAGRSRLRAAADARRWDHVYQ
                     RAQQSRRH"
     gene            complement(5182..5946)
                     /gene="map"
                     /locus_tag="VF995_08955"
     CDS             complement(5182..5946)
                     /gene="map"
                     /locus_tag="VF995_08955"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX9343727.1"
                     /translation="MVILKSPDEIATMREAGRVVARALAAVAAAVRPGVSLRELDALG
                     EQVIRDAGAKPSFLGYNPHFASSPFPGTLCLSPNQVIVHGIPDRTVLRDGDVLSIDCG
                     AIVDGYHGDAAVTVPVGQIDEAARRLLETTERSLRAGIEQVRPGGRLSDISHAIGQVA
                     HKAGYGIVPDFGGHGIGRALHEDPSVLNDGRPGRGLRLREGLVLAIEPMFNQGSGDYD
                     VKPDGWALVTRDGSRSAHFEHTIAITAAGPLVLTLP"
     gene            complement(5970..>7110)
                     /locus_tag="VF995_08960"
     CDS             complement(5970..>7110)
                     /locus_tag="VF995_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9343728.1"
                     /translation="ERVDVDNFALSATAPPLVDDGGVTYFTLDRGAVALTRFPLDPSG
                     GSRAGAGAGAGPGPVGEPIPLLSGQRQVTDFAAARTPAGTVVAAVVTDAASSGELVVL
                     GPDGTERVVTGFGANLARAAALRPMEELAASAPDGYPVHGWVVKPAGKPPFPVLLVIH
                     GGPFTQFGYRLFDEAQVYAGAGYAVVLGNPRGSAGYGEAHGRAIVGDFGNLDAQDLQA
                     LLDAALADPELDATRVGVMGGSYGGLMTGWLLGHSDRFRAGISERACNAFDSFEGSSD
                     IGWLFNKDYIGTDPERVRAQSPLTYADSISAPVLIIHSEQDWRCPLEQAQRLFVALKQ
                     RGVETEMLLFPGEGHELSRSGLPSHRVARFAAILDWWERHLKPAE"
BASE COUNT          921 a         2510 c         2649 g         1030 t
ORIGIN      
        1 tcccggagct gggcttctgc ctcaccaccc gcgggcagat gttctggctc accgagggcc
       61 tccccaactc cctggaaccc ggcaaacgcc cccggacgac gctcagcccc tcgctggcgc
      121 tgcgcgacgg ccagccgtgg ctcgcctgcg ggacccccgg cggcgaccag caggaccagt
      181 ggtcgctgca gttgctgctc aaccacctcc acttcggcat ggacctgcag gaggcgatcg
      241 acgcgccggc cttccacagc gatcatttcc ccagctcgtt ctacccgagg gagggccgcc
      301 ccggcgtggt cgtcatcgag cggcgcgccg gcggcgccgt gatcgaggcg ctgcgcgccc
      361 gcggccacca ggtcgaggtc gccgacccct ggtcgctcgg ccgcctctgt gcggtcggcc
      421 gcgatccgga aaccggcctg ctgatggccg ccgcgaatcc tcggggtcgc gcgggttacg
      481 cggtgggtcg gtagcggcgc ccgcgctcca cccgaacgcg cgggctacca gagtgcgatg
      541 cggggctcgc ggaccgtgcg ggtgacctcg tcgagggcgt tctgcaggtc catcggggag
      601 acccgtgggg ggttgtcggc ggcgaagcgg gtgccgcgcc ggaggccgtc gcgccactgg
      661 cggcggcgct cgttgggggg catctggtcg gcgcggagca tctcgctggc gccggtgacc
      721 acgaactcct ggtggctgcg gatgccccag gcggcaccgc cgcgctggct ggcggcgacg
      781 aagaaggcaa aatacgagcc gatcaggtcg gccagggacg agggcgtctg tccccgcgcg
      841 ctggcgtcgg tgcgggcggc caggcgcggg tcgggcatgt ggtagaggtg gccgccgttg
      901 cggctcagcg ccaccgccag gtagaggtgc tcgggcgagg ccgccggcca gctcgacagg
      961 tccgggaacc cgccggcctg ccgcagcgtg gcggcgtcga ccaggcaggt gccgttcatc
     1021 agctcgaccg ggaacggcgc cagcagcgcg ctctcgttgt cgagcagctg cgggtcgcgc
     1081 aggtactcgg cggggtagca gtcgaaccgc gcggagaagc gggcggcctc gggctccagc
     1141 cgcccgatgt aggccaggtc ggcgatctgc tgcggccaca gcgagcggtc atacgaaggg
     1201 agcgcgagcg cgcaggcgcg cgggtcgtgg tcgcgcagca gcgtcagggt gtaggcggca
     1261 ccggtgacgc agtgcggccg gaacaccgcg tcgtcgtcgc cgaacagcac caggtcgccg
     1321 gtggcctggg cgatgccggt gttgcgcccg gcaccgaggc cacggcggcg cttgtgccgc
     1381 agggtcacga cccgggtggt ccgctcggtg gaggcgatca tgtagcggta ggcctccacg
     1441 atcatcgggg tgtggtcggt ggagccgtcg tcgaccacca cgatctcgcg cagggcctcc
     1501 ccggtttggg cgagcaggct gtcgagcgcc caggtcagcg ggttgtcggc gagccggccg
     1561 ctgttcgccg cgaagggaca ctggttgtag gtgaccagca ccgccgagac gcgctggcgg
     1621 atcgcgggga cgttgatcgc cctggtgagc tggacggggt tctggctctt cagccagtcc
     1681 cgcgcttcga gcagcggttg gggttgcatc tcggacgctc catctcatgc atggcacccc
     1741 accggcccgc acgatttccg ccgacagatc atctctgatc gaatctgcca ggtatgcggc
     1801 cggtcacgac gggccgaagc ttactcggtt ttcgccactg gcacgattcc gcttgcctgc
     1861 ttggcaatac tttgccgccc acttcggccg gcttcgtcgc atcgtgacgt tcgggtatca
     1921 ggcagactcg tggaggtgct cgtcgagcga gcggaccacc cgcagatcgt gccagcgcgc
     1981 gaggtgctcg cggtggacgt tgcggatgcg gcgcagcacg atcgggaact tgccgcgctc
     2041 ggcgtggccg caggcctcct ccagcgtggc gcaggccgct tcgatctcgc cctggaggac
     2101 gcgggcgatg ccgaggtcga ccttcacgcc gaggcggtgg cggtggaggg acaggtcggc
     2161 tgtctccagt gacttggtga gcgcctgctc ggccgcttcg ccccgaccga gcagcagatt
     2221 gcacagccca acgtagccgt ccacccgggt ctgctcccag taggccagga agccgtgctc
     2281 ctcgccgggg cggatctcgg ccagggcgtc ctcggcgcgg tccagctccc ggcgggcggc
     2341 gtcggcctcg ccacggaagg cgtgctcggc ggcgcggcag ccagccgccc agccgcgcat
     2401 cggtgcggcc aggcgggcac cgccggggcc ggcgccggct gctcgcagga gggtcagcgc
     2461 cttggtgggg ttggtctgcc tgccggccgc tgcgtagcgg gtggaataca ggtagctcat
     2521 cgggatgaag gtctcggcga gcagctgctg gtcgccggtc tggcgggcga ggaggcgggc
     2581 gagggcgtag tccgcccgcg ccgcgtcgcg gtcctcgcac tggaaggtgg tccagccggc
     2641 gaggaccgcg gcctcgccgg tgatcacccg caggcgccgc tcgatgctct caggctgcga
     2701 gccgtgcagg agcccgacga ggtaatcgag gtgggcccgc gccggctcgg cgagccgcac
     2761 cgtggggctg ctcgccagct cgccggcgta ggccatggtc gtcgctcgca agtctcgcag
     2821 aagctccagg tcgacccggc tcgggttggc tctggcccag gcgagccgct cggggtcgac
     2881 tgccggcagg agcttctcgg cgaccaccag cccgccgagc atgaccacca cgcgtcgctt
     2941 caagccgtcc tcctcgtcga cctcgatcgt gggatggttg ccgccgggca gctcgacgtg
     3001 gaccgtgcgg ccgccggcgc tggtgtggat ctcgacgtgg ccgccctggg ggtcctctga
     3061 tccaggcgag tcgccctcgt ccaggctgta gtcgtggccg aagccgagct tgtcctgccg
     3121 ggtctcgtag aagcgggcga ggtagtcacg ggcttcctcg ccggggcggc ggcccgagcg
     3181 cggccagtgc tcccactcgt gcagccgtga ggtgttgagg ccgggtgggg gcatgagccc
     3241 gtcgtcactg taaagctggc cagcaccccg gacaacctgt gccagtgtca ggccgcgggc
     3301 gagccggtgc gcctgcagcg ggctgatctc gggggaggcg gcgaggatct cgcaaacgat
     3361 cgcgccgggc gccagcccgg ccgcctccca gtcgcgccgc agcacgtcaa tccgcttgta
     3421 gagcgcccgc gaggtatcag atcgcaacgc cagcagcccc cctcggcacg gagcgcaacg
     3481 agtcgcgtcc tccgtgtttc gatcataaga gaatggagag gctgattgcg aaaccgcacg
     3541 ttcaggtatt tctcccatag catcacctat gaccaatatg gccgatgcgg atcctgctga
     3601 tcggacctga ccacgaccgc gggagcatcc cgccctacct cgacctgctc gccgccgggc
     3661 tgggggagcg gggcgcccag gtgacacggt tgagcgcggg cgggttgccg ctcgaccggg
     3721 aatccgctcg ggaatccgct cgggaatccg cggccccgcg tttctggtcg gcggagcgga
     3781 tcgtcgccgc ggccgaggcg ctggtggcgc ggatcgaccc ggacgccttc gacctggtct
     3841 cgctgcacta cggcaacctg gaggtcgagc agctggtccc ggtgctgtgg gaacggcgct
     3901 tcggcccatc cgggtccggg tccggcccgc cgatcgtggc ccacgcgcac ttcgtcgact
     3961 ggacgctgtt ctccacccat gtggtggact cggcgctcta ccaggcggtc agaactggga
     4021 tcgggcgcat ggacggcctg gtgttcttcg gcgagtacgc ccgccgcgcg ctcctcgacc
     4081 gtgacggcct gcgcggcgtc ccaacgctgg tgagctactt ccccaccacc atcccggcgg
     4141 gggagcgagc ccccggcccc ggcgcgctgc ggcccgccct gcgcgagctg gacccggcct
     4201 cgcgtgccgg ccgggggcag ggcggcggcc tcctccccct ggccaccatg tacgggttcg
     4261 ccaacccctg gaaggacccg agcgacctgc tggccgcgtt ccagcggatg cgggtgccgc
     4321 tgatggtcct gctggccggg catggctggg accggcccga gcgggccggc gccgacctcc
     4381 gcgcggcgct gtctccgcgc cgcctgcgga tcggtccggt tgagctgacc gtgctggccg
     4441 actacatcgg gccgggtgag cgcctcgcgc tggtggcggc cagcgacttc gcggtgttcc
     4501 cctaccgccg gcacgagagc tttcagggca gcggcgcgat caccgactac ctggccaacg
     4561 gcgtgccggt ggtcgccacc gacgtcgcca acatggccga gctgatcggc gacgccggcg
     4621 tggtggtccc gcccggcgac ccggcggccc tggcggccac gctcgaccgg gtggcgggcg
     4681 acgccggcct ccgcgcggcg ctcgccctgg cggcccggcg gcgggcgccg cgcttctcgc
     4741 tcgccagaca cctcgacgac tgcgacgcct tctaccgcac gctggccgcc cgctgaccgc
     4801 tgaccgccgc gcgtggcggg accgctacac cgccatggtg atagtcaaca aattgggggc
     4861 cgtgcgaccc tccgttcttg ggtcggtcgt gccttgcgag gggcatgaca ggatcgccca
     4921 ggaggggcag cgaaatgatg gaaagcatcg tggcggccac gctcttgctg gccgtggtgc
     4981 tggtggcata cctggccgac gtgctcgccg gtcgcagccg cctgcgcgcg gcggccgacg
     5041 cccgccgctg ggaccacgtc taccagcggg cccagcagag ccgccgccac tagcggcggc
     5101 gggacgcagc cgtcaacact ccgggtcccg gccgttgcca atcccccaat ggccggggct
     5161 cggatctctt tgttgctgag gctacggaag ggtgagcacc agcgggccgg cggcggtgat
     5221 cgcgatggtg tgctcgaagt gggccgagcg ggagccgtcg cgggtgacca gggcccagcc
     5281 gtccggcttg acgtcgtagt cgccggagcc ctggttgaac atcggctcga tcgccagcac
     5341 cagcccttcg cgcaggcgca gcccgcggcc ggggcggccg tcgttgagga cgctcggatc
     5401 ctcgtggagg gcgcggccga tgccgtggcc gccgaagtcg ggcacgatcc cgtacccggc
     5461 cttgtgggcg acctggccga tggcgtggct gatgtcggag aggcggcctc cggggcggac
     5521 ctgctcgatc cccgcccgca ggctgcgctc agtggtctcc agcaggcggc gggcggcttc
     5581 gtcgatctgg ccgaccggga cggtgaccgc cgcgtcgccg tggtagccgt ccacgatcgc
     5641 gccgcagtcg atcgacagga cgtcgccgtc gcgcagcacg gtccggtcgg ggatcccgtg
     5701 gacgatcacc tggttgggcg acaggcacag cgtccccggg aacggcgacg aggcgaagtg
     5761 cgggttgtag ccgaggaacg acggcttggc gcccgcgtcg cggatcacct gctcgccgag
     5821 cgcgtccagt tcccgtagcg agacccccgg ccgcaccgcc gccgccaccg ccgcaagggc
     5881 ccgcgcgacc acccgcccgg cctcccgcat ggtggcgatc tcgtcagggg atttcaggat
     5941 gaccacggct gttcgccccc ccttgctcct tactccgctg gcttgaggtg ccgttcccac
     6001 cagtcgagga tagccgcgaa gcgggcgacc cggtgactgg gcaggccgga gcgcgacagc
     6061 tcgtggcctt cgccgggaaa cagcagcatc tcggtctcga ccccacgctg tttgagggcc
     6121 acgaacaggc gctgggcctg ctccagcggg cagcgccagt cctgctcgga gtggatgatc
     6181 agcaccggag cgctaatgct gtcggcgtag gtgagcgggc tctgtgccct gacccgttcg
     6241 gggtcggtgc cgatgtagtc cttgttgaag agccagccga tgtcgctcga cccctcgaag
     6301 ctgtcgaagg cgttgcaggc gcgctcgctg atgcccgcgc ggaagcggtc cgagtggccg
     6361 agcagccagc cggtcatcaa gccgccgtag gacccgccca tcaccccgac ccgggtcgcg
     6421 tccagctcgg ggtcggcgag ggcggcgtcg agcagcgcct gcaggtcctg cgcgtcgagg
     6481 ttgccgaagt cgccgacgat cgcgcggccg tgggcctcgc cgtagccggc cgagccgcgc
     6541 gggttgccca gcaccaccgc gtagcccgcg ccggcgtaga cctgcgcctc gtcgaacagc
     6601 cggtagccga actgcgtgaa cggcccgccg tggatcacca gcagcaccgg gaacggcggc
     6661 ttgccggcgg gcttgaccac ccagccgtgc acggggtagc cgtccggggc gctggccgcc
     6721 agctcctcca tcggccgcag cgcggccgcg cgggccaggt tcgcgccgaa gccggtgacc
     6781 acccgctcgg tcccgtccgg gccgaggacc accagctccc cgctggaggc ggcgtcggtg
     6841 accaccgcgg cgacgaccgt gccggcaggc gtccgggccg cggcgaagtc cgtcacctgg
     6901 cgctggccgc tgagcagcgg gatcggctcg ccgacgggtc cagggccggc cccggccccg
     6961 gccccggccc gggagccgcc agacggatcg agcgggaagc gggtcagggc gaccgcgccc
     7021 cggtccagcg tgaagtaggt gacgccaccg tcgtcgacca gtggtggcgc ggtcgcgctc
     7081 agcgcgaagt tgtcgacgtc gacgcgctcg
//
LOCUS       DASWDC010000278         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_908538, whole genome shotgun sequence.
ACCESSION   DASWDC010000278 DASWDC010000000
VERSION     DASWDC010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908538"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VF995_08965"
     CDS             <1..715
                     /locus_tag="VF995_08965"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX9343729.1"
                     /translation="KLVFSWLGNPGVGSLHAVRRRVERGDPESLEIEEYSHFGMIGRY
                     TAGAANLPFYPLRSYAESDLPRVNPNIRPVSSPFGGETIYAVPPLNPDVAIIHAQRAD
                     RAGNTQIWGLLGCQREAAFAARRVIVVVEELVDEAVIRSDPNRTLIPGLKVAAVVVCP
                     RGAHPSYAQGYYDRDNRFYLEWEQISRDPARLDAWLDEWVHGTRDHAEYVAKLGEPRW
                     ESLTPAPALSAPVDYGDYR"
     gene            737..1504
                     /locus_tag="VF995_08970"
     CDS             737..1504
                     /locus_tag="VF995_08970"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX9343730.1"
                     /translation="MSDWSRSEMLVVAGARALAGKRVCFVGVGLPNIAVALAQRTVAP
                     EIELVYESGVYGARPARLPLSIGDPCLVTGATATMGMVELFQYYLQGGLVDVGFLGAA
                     QLDRYGNINTTVIGDYQRPKVRLPGSGGACEIAINAAEVFILMRQSARSFVETIDFRT
                     SPGNLGGAALRAAQGWQGRGPTLVVTDLARYRFDRDSGEMLLESIHPGVTLDEVRAAT
                     GWPVKIAGDLAETPAPSAEELRLIREELDPEGVYVKR"
     gene            1565..1936
                     /locus_tag="VF995_08975"
     CDS             1565..1936
                     /locus_tag="VF995_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9343731.1"
                     /translation="MDWKLELVALPVSDVDRAKRFYTEQAGFNADHDYTVSEEIRFVQ
                     LTPPGSACSIAIGTGLVDSPPGSVRGVQLVVQDIEAARAELAGRGVAVSEVQDFPWGR
                     FVFFSDPDGNAWSVQQIPHRG"
     gene            2045..3034
                     /locus_tag="VF995_08980"
     CDS             2045..3034
                     /locus_tag="VF995_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX9343732.1"
                     /translation="MPLPDSDSERPGSRLPAAAPPPPWRWRRGLRVLLVPLGAAALLA
                     LAAVGALRSPPGTGRGEAHFPTALIESLLLVAGVAVLLAVVAAVYALGPDHRRRQLAA
                     RRSAWTLLLFPVLLTLAFWLLRHAPRRWLRLLPAQPATAPSQPQPGPAGSLSHPQAEW
                     LPVLLLGGLLMAAAAGIAWGAWRRRRQLLPPSARKLAAMLGEVLDDVGAEPDPRRAVI
                     AAWAGMERRFAAAGLPREPAETPLEYVARILDHAVPVAAMPVRTLADLFEQAKFSHHL
                     IDQGMRAQALAALRAIREDLEAAAAAEEAAATMAAQAARTQPPEKRGAAWRAH"
     gene            3019..3582
                     /locus_tag="VF995_08985"
     CDS             3019..3582
                     /locus_tag="VF995_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343733.1"
                     /translation="MAGALTRTALIAVAATLLLSAAVEAMPQRRPLVISAYLLLLGAL
                     TLRLLVRWTWQTYPRYRRSELDAALTWRPTPPAPAAALVAATRTLTLSHASAGYAHSR
                     LRPHLRALAADLLTWHRAIALDQEPERAAAALGPVAWSWLRPDRAEPADRDDPGLPLE
                     ALGEIIVSLERLEAPEPAAPLEGVARR"
     gene            3687..>4052
                     /locus_tag="VF995_08990"
     CDS             3687..>4052
                     /locus_tag="VF995_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9343734.1"
                     /translation="MLDQVERAVVGKRRALELVLLGLLADGHVLLDDYPGLAKTLIAR
                     SFAQVTRLRFARVQFTPDLMPSDVTGSSIYNQRLADFEFRAGPVFTNLLLGDEINRSP
                     PKTQAALLEAMQEHQVTADG"
BASE COUNT          541 a         1533 c         1433 g          545 t
ORIGIN      
        1 caagctggtg ttctcctggc tcggcaaccc cggcgtcggg tcgctgcacg cggtccgccg
       61 ccgggtcgag cgcggcgacc ccgaatcgct tgagatcgag gagtactccc acttcgggat
      121 gatcggccgc tacaccgccg gcgccgccaa cctccccttc tacccgctgc gctcctacgc
      181 cgaaagcgat ctgccccggg tcaaccccaa catccgcccg gtcagctcac cgttcggcgg
      241 cgagacgatc tacgcggtgc cgcccctcaa ccccgacgtc gccatcatcc acgcgcagcg
      301 ggccgaccgc gccggcaaca cccagatctg gggcctgctc ggctgccagc gcgaagccgc
      361 cttcgccgcc cgccgggtca tcgtcgtcgt cgaggagctg gtcgacgagg cagtgatccg
      421 ctccgacccc aaccgcaccc tgattcccgg cctgaaggtc gccgcggtgg tggtctgccc
      481 ccgcggcgcc cacccctcct acgcgcaggg ctactacgac cgcgacaacc gcttctacct
      541 cgaatgggag cagatcagcc gcgaccccgc ccgcctcgac gcctggctcg acgagtgggt
      601 ccacggcacc cgcgaccacg ccgagtacgt cgccaagctc ggcgagcccc gctgggagtc
      661 cctcaccccg gccccggcac tgtccgcccc ggtcgattac ggcgactacc ggtgacggcg
      721 ggaacagagg tgccccatga gtgactggtc gcggtcggag atgctggtgg tggcgggcgc
      781 acgagcgctg gccggcaagc gggtctgctt cgtcggggtc gggctgccga acatcgcggt
      841 cgcgctggcg cagcggaccg tcgcgccgga gatcgagctg gtctacgagt cgggggtgta
      901 cggcgcgcgg ccggcgcggc tgccgctgtc gatcggcgac ccgtgcctgg tgaccggcgc
      961 gaccgccacc atgggcatgg tggagctgtt ccagtactac ctgcagggcg gcctggtcga
     1021 cgtcggcttc ctcggcgcgg cgcagctgga ccgctacggc aacatcaaca ccaccgtgat
     1081 cggcgactac caacggccga aggtgcgcct gcccggctcg ggcggggcct gcgagatcgc
     1141 catcaacgcc gccgaggtgt tcatcctgat gcgccagtcg gcgcgctcgt tcgtcgagac
     1201 gatcgacttc cgcaccagcc ccggcaacct cggcggcgcc gcgctgcgcg ccgcccaggg
     1261 ctggcagggg cgggggccga cgctggtggt gaccgacctg gcccgctacc gcttcgaccg
     1321 cgacagcggc gagatgctgc tggagtcgat ccaccccggc gtgaccctcg atgaggtgcg
     1381 tgccgcgacc ggctggccgg tcaagatcgc cggcgacctc gccgagaccc ccgcgccgag
     1441 cgccgaggag ctgcgcctga tccgcgagga actcgacccc gaaggcgtct acgttaaacg
     1501 atagcgacag aaacgccgat cctatccata tcacaccgat gacaccgacc aaaggatggc
     1561 gcggatggac tggaagctcg agctggtggc actgcccgtc tcggacgtgg accgcgcgaa
     1621 gcgcttctac accgagcagg ccggcttcaa cgccgaccat gactacaccg tcagcgagga
     1681 gatccgcttc gtgcagctga ccccgcccgg ctcggcctgc tcgatcgcga tcggcacggg
     1741 gctcgtcgac agccctcccg gctcggtacg gggcgtccag ctcgtcgtcc aggacatcga
     1801 ggccgcccgc gctgagctgg ccgggcgggg cgtggcggtg agcgaagtcc aggacttccc
     1861 ctggggccgc ttcgtgttct tcagcgaccc ggacggcaac gcctggtcgg tgcagcagat
     1921 cccgcatcgc gggtaaccgg gcgggtgacc gggcggggcg tcgtatccgg gcgctgacgc
     1981 cgggccggcc gcgccggtat gctccacaga ccccggccaa cgacgtctgg aggaggccgg
     2041 tcagatgccc ttgccggact cggactcgga gcgtcccggc tcgcgattgc cagcggcagc
     2101 gccgccgccg ccgtggcggt ggcggcgcgg gctgcgggtc ctgttggtgc cgctcggtgc
     2161 ggctgcgctg ctggccctgg cggcggtcgg ggcgctgcgc tcgccgccgg ggactgggcg
     2221 cggcgaggcg cacttcccga ccgcgttgat cgagtcactg ctgctggtcg ctggcgtcgc
     2281 tgtgctgctc gccgtggtgg cggcggtcta cgcgctcggc ccggaccacc gccggcggca
     2341 gctggcggcg cggcgcagcg cctggaccct gctgctgttc ccggtgctgc tcaccctggc
     2401 gttctggctg ctgcgccatg ccccgcgccg ctggttgcgt ctgctcccgg cgcagcccgc
     2461 caccgcgccg tcgcagccgc agcccggacc ggccggcagc ctcagccatc cccaggcgga
     2521 gtggctgcca gtgctcctgc tcggcgggct gctcatggcc gccgctgcgg gcatcgcctg
     2581 gggcgcgtgg cggcgccgcc ggcagctcct gccgccgagc gctcggaagc tcgccgccat
     2641 gctcggcgag gtcctcgacg acgtcggggc ggagcccgac ccgcgtcgcg ccgtgatcgc
     2701 cgcctgggcg ggcatggagc ggcgcttcgc cgccgccggt ctgccgcgcg agccggccga
     2761 gaccccgctc gaatacgtcg cccggatcct cgaccatgcc gtcccggtcg cggcgatgcc
     2821 ggtgcggacc ctcgccgact tgttcgagca ggcgaagttc agccaccatc tgatcgacca
     2881 gggcatgcgt gcgcaggccc tggcagcgct gcgggcgatc cgcgaggacc tcgaagcagc
     2941 agccgccgct gaggaggcgg ccgcgaccat ggcggcgcag gcggcgcgga cgcagccgcc
     3001 ggagaagcgg ggagcagcat ggcgggcgca ctgaccagga cggcgctgat cgcggtcgcg
     3061 gcgaccctgc tgctgagcgc ggcggtcgag gccatgccgc agcggcggcc gctggtgatc
     3121 tcggcctatc tgctgctgct cggcgcgctc accctgcggc tgctggtgcg ctggacctgg
     3181 cagacctacc cccgctaccg gcgctcggaa ctggacgccg ccctgacctg gagaccaacc
     3241 ccgccagccc cggcggcggc gctggtggcg gccacccgca ccctcaccct gtcacacgcc
     3301 agcgccgggt acgcgcacag ccgcctacgc ccgcacctgc gggcgctcgc ggccgacctg
     3361 ctcacgtggc accgggcgat cgccctcgac caggaacccg agcgggcggc cgccgcgctc
     3421 ggcccggtgg catggtcgtg gctgcggccc gaccgggcgg aaccagccga ccgcgacgac
     3481 cccgggctgc ccctggaggc gctcggcgag atcatcgtca gcctcgagcg cctggaagca
     3541 ccggaaccgg cagcgccgct ggagggggtg gcgcgccggt gacccagcac gacggcccct
     3601 ccaacgccaa cgccagccac caggccagcc gcgaggccgg ccgccagatc cgcggcgtcg
     3661 cggacgtccg ggacctggcc accgagatgc tcgaccaggt cgagcgggcg gtggtcggca
     3721 agcggcgcgc actggagctg gttctgctcg gactgctggc tgacgggcac gtgctgctgg
     3781 acgactaccc ggggctcgcc aaaaccctga tcgcgcggtc gttcgcgcaa gtgaccaggc
     3841 tgcgcttcgc gcgcgtccag ttcactcccg acctgatgcc ctccgacgtc accggctcgt
     3901 cgatctacaa ccagcgcctc gccgacttcg agttccgggc cggcccggtg ttcaccaacc
     3961 tgctgctcgg cgacgagatc aaccgctcgc cacccaagac ccaggcggcg ctgctggagg
     4021 cgatgcagga gcatcaagtg accgccgacg gc
//
LOCUS       DASWDC010000279        10027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1496856, whole genome shotgun sequence.
ACCESSION   DASWDC010000279 DASWDC010000000
VERSION     DASWDC010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496856"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VF995_08995"
     CDS             complement(<1..467)
                     /locus_tag="VF995_08995"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="binding of PriA to forked DNA starts the assembly
                     of the primosome, also possesses 3'-5' helicase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosome assembly protein PriA"
                     /protein_id="HEX9343735.1"
                     /translation="MIDRRVSAGVQVVVDVPLFHLDRPFSYRVPEALRADVSLGSRVK
                     VPFGGRKRVDGWVVGFVDDDELPADAREILRVVSPLPAFDADGLELLRWVAARYAGTL
                     IDVVRLAVPPRVAAIEKQPQEAVGGPDGAAAPEAAVGPGPGPAPSFGDPLVARV"
     gene            complement(559..1752)
                     /gene="metK"
                     /locus_tag="VF995_09000"
     CDS             complement(559..1752)
                     /gene="metK"
                     /locus_tag="VF995_09000"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX9343736.1"
                     /translation="MAKRYLFTSESVTEGHPDKMADQISDAVLDEILTSDPMGRVACE
                     TLITTGLVVVAGEISTSTYVDIPSVVRKTILDIGYDRAKYGFDGSTCGVTVAIDEQSP
                     DIAQGVDHAWEHRVGDVDPLDVQGAGDQGMMVGFACDETPELMPVPVALAHRLSRRLA
                     QVRRSGDIPYLRPDGKTQVTVEYESGRPRRLDTVVVSTQHGPDIDLDTLLTPDIREQV
                     IDPTLPEDLGAEGLRVFVNPTGRFELGGPCADTGLTGRKIIVDTYGGMARHGGGAFSG
                     KDPTKVDRSAAYAVRWVAKNVVAAGLANRCEVQVAYAIGVAHPVSVFVETFGTEQVDP
                     DKLGKLIPEFFDLRPAAILRDLDLRRPIYRKTAAYGHFGRTDPDFTWESTSRADELAK
                     AAGSL"
     gene            complement(1874..3025)
                     /locus_tag="VF995_09005"
     CDS             complement(1874..3025)
                     /locus_tag="VF995_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEX9343737.1"
                     /translation="MGDRIELKAFCSDPEATAEAVRDFGAMESGASEERDTYFHVRRV
                     PRGRLLLRRTAHQAARLIAYTLANQPGIHRTPAIVAELPATGADALEAVLAAANGVRV
                     VVEKQRRTFLHKQVLVHLDQVRELGSFIEVVVLADRCESEEAADTIASEVRSALGIGD
                     ADVVAWTYADFATIQAAGRSWRQRLQQTASSGVVPGTLFVLDGPSGAGKTTIAQLLAD
                     RVPGLDFIRRYTSRPPRQTVTESEYIFVGGEQFSQLIANGELIEFRDFEFQMSYGLPW
                     QPVMDSLLAGRDALGIINLGNVRLVKQVVPEARTILLSVTPETLRRRLEARGKHNEGE
                     IAERLGNAARVDAYRPYYDDVVDNREGELAQVVDELAVHLRTRDQPGSS"
     gene            complement(3254..4468)
                     /gene="coaBC"
                     /locus_tag="VF995_09010"
     CDS             complement(3254..4468)
                     /gene="coaBC"
                     /locus_tag="VF995_09010"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX9343738.1"
                     /translation="MLLGVSGGIAAYKAAELARELMRAGAQVQPLLTAAAEALVTRAT
                     FAALTGRQVPASIWDDPAAVEHVELARWGQVLMVAPATAHTLARLAAGFADDLLTNVA
                     LAFPGPVVVAPAMHTEMWRHPATQANLAVLERRGVQVVLPASGPLTSGDVGLGRLAEL
                     SDLVAGVAVALKDRDSPAEARTLAGVRMLISLGGTREPLDPVRYLGNRSSGRMGAAIA
                     VEALRRGAEVTAVAAVTSVDLPAGTRVLQVETAQQLYDTVLAEAPAHDVLIMAAAVAD
                     FRPKVSASVKLKKDQGVPELALEPTPDALAELGRRRRPDQVLVGFAAETDDHLANARA
                     KLDRKRLDLVVVNHVEPDRSAFGADAADAALVDATAAEDLPAGASKQEIARRLLHRVE
                     RLRAQRRPPRPA"
     gene            complement(4524..4775)
                     /gene="rpoZ"
                     /locus_tag="VF995_09015"
     CDS             complement(4524..4775)
                     /gene="rpoZ"
                     /locus_tag="VF995_09015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX9343739.1"
                     /translation="MIEPKIDDLLARVDSKYTLVILAARRARQINGYYSQLGEGIGEF
                     VPPMVDAGPNRKALSVALQELDEGKLEWERPAETAAAIK"
     gene            complement(4911..5228)
                     /gene="mihF"
                     /locus_tag="VF995_09020"
     CDS             complement(4911..5228)
                     /gene="mihF"
                     /locus_tag="VF995_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX9343740.1"
                     /translation="MPLPTLTPEQRQQALEKAAEARRKRAELKAQLKSGNLTLTTVLD
                     KEDDDTVGKMKVASVLESLPGVGKVRARKIMEKHDISASRRVRGLGSKQKDALLAEFG
                     TKK"
     gene            complement(5430..6098)
                     /gene="pyrE"
                     /locus_tag="VF995_09025"
     CDS             complement(5430..6098)
                     /gene="pyrE"
                     /locus_tag="VF995_09025"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX9343741.1"
                     /translation="MTPRQAGSQPPAPPPLPPGPGPLDADAVLALYQHAGGLRRGHFV
                     LSSGLHSDTYLQSAPVLQWPPAAEALGRSLASRWHSQAAGGPVDVVVGPAMGGVVIGH
                     EVARALGVRMVFTERTGDRGAGGAMALRRSFTVGPGERVLIAENVVTTGGSALEVAEL
                     LRTAGAAVAGLVTIIDRLPAGARLPVPFTALARIEAGAWESAACPGCLQDANAETPGS
                     RGRI"
     gene            complement(6154..6894)
                     /gene="pyrF"
                     /locus_tag="VF995_09030"
     CDS             complement(6154..6894)
                     /gene="pyrF"
                     /locus_tag="VF995_09030"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX9343742.1"
                     /translation="MGSGTASGSGSAIVLALDTPDLAEARALAAATSGSVGLVKVGLE
                     LFGAHGPDAVRTLRADGHRVMLDLKLHDIPATVGRAVAALVPLGAELLTVHAGGGTAM
                     LTAAVQAARAAGPGPGPRIAAVTVLTSLGPDDLAALGAGDATRAATRLTRLALEAGCQ
                     ALVCSPLEVARLRALTGPDVTIICPGVRPAAPQPDSDDQARVATPAAALAAGADLLVV
                     GRPITRAPDPAAAAATIAAETQTAGAAR"
     gene            complement(6917..7855)
                     /locus_tag="VF995_09035"
     CDS             complement(6917..7855)
                     /locus_tag="VF995_09035"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX9343743.1"
                     /translation="MSMTIPDPDLTVELCGVRLPSPVLVASGCFGFGREMARFMDLGE
                     LGAVVTKSVSLEPRPGRPTPRIAETPAGMLNAIGLQNPGVDAFRASELPFLAQAGARV
                     VASVVGRSAEEFGRVAEQFSGEDAVTLLEVNLSCPNVDHRGEVFATDPDAAASVIRAV
                     KAVAGQPVLAKLTADVTDLVRVARAVVDAGADGLTLINTLLGMAVDVHRRRPKLAAVT
                     GGLSGPAIRPVAVRCVWQVAKALPGVPIVGTGGIMSADDAAEFLLVGATAVAVGTGNF
                     VSPRRPLEITDGLRRYLAEQGVATVAELPALFKAER"
     gene            complement(7858..8709)
                     /locus_tag="VF995_09040"
     CDS             complement(7858..8709)
                     /locus_tag="VF995_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX9343744.1"
                     /translation="MAEVLGHRRAGVYHTLTLAASEVARTAQPGQFIGLLTGPERAYL
                     LRRPFSIHRVDWRGAGSGSLEVVFDVVGPGTRALSWLRPHDTVDLVGPLGHAFTLPEQ
                     PTSCLLIGGGYGSAPLFFLAERLLARGCRIDFIVGASTEERLFKAMEAKRLARSAVIT
                     TDDGSAGRRGIVTDPMPEVIEAARTELVYACGPMAMLAAVSQVATTAKVACQVAVEEL
                     MACGTGICYSCVVPMAAPEGGIRMARSCVEGPVLDGAQVAWAELGLSAGLSGGPGYPA
                     SLAEEAM"
     gene            complement(8766..>10027)
                     /locus_tag="VF995_09045"
     CDS             complement(8766..>10027)
                     /locus_tag="VF995_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX9343745.1"
                     /translation="AEDRGRFGTVLAELGLDAPAWGEAASREEALAVAERIGYPVLAR
                     PSYVLGGRSMEVSYDAEALTRSAALATMDHGSRPVLIDRFLEDAVEVDVDALCDADGN
                     VLVCGVMEHIEEAGVHSGDSACVLPAITLGTDQVAEVAAATAKLARALGVVGILNVQF
                     AFKAERLYVLEANPRASRSVPFVEKVTGLPVAKAAALLMAGARLPELRQPGGMLPLGE
                     GILAPEPRHVGVKEAVLPFARFPGADSLLGPEMRSTGEVLGLDQSFGAAFAKSQEASY
                     GALPTKGVAFLSVRNADKRAIVLPAKRLADLGFTLVATAGTASVLNRNGVPARVVRKV
                     HEGPGNVVDLIRGGEIDVVVNTPHGSGPRTDGYEIRTAAVAADIPCVTTLSGLAAMVQ
                     GIEARLAGELTVQSLQAYHQRGSEGDA"
BASE COUNT         1401 a         3432 c         3810 g         1384 t
ORIGIN      
        1 acccgcgcca ccagcggatc cccaaacgac ggcgccggcc cggggccggg cccaacggca
       61 gcctccggcg cggcggcccc gtcgggaccg cccacggcct cctgcggctg cttctcgatg
      121 gcggcgaccc ggggcggcac cgcgagccgc accacgtcga tcagggtgcc ggcgtagcgg
      181 gccgcgaccc agcgcagcag ctccagccca tcggcgtcga acgcgggcag cggcgagacc
      241 acccgcagga tctcacgagc gtcggcgggc agctcgtcgt cgtccacgaa cccgacgacc
      301 cagccgtcaa cccgcttgcg ccccccgaac ggcaccttga ccctgctgcc gagcgacacg
      361 tctgcccgca gcgcctccgg caccctgtag ctgaacggcc gatcgagatg gaacagcggc
      421 acatccacca cgacctggac gccggccgac acgcggcggt cgatcatgcc agcgagtcta
      481 cgccgctcgg gttggctggc gggaccccga agaatcccgc cagagggtcc gctgtcctcg
      541 ctggttccag ggtccccgtc agagggagcc ggcggccttg gcgagctcgt cggcgcggct
      601 ggtcgactcc caggtgaagt cggggtcggt gcggccgaag tggccgtagg cggcggtctt
      661 gcggtagatc gggcggcgca ggtcgaggtc gcggaggatc gcggcggggc gcaggtcgaa
      721 gaactcgggg atgagcttgc cgagcttgtc ggggtcgacc tgctcggtgc cgaaggtctc
      781 gacgaagacg ctgacggggt gggcgacgcc gatggcgtag gcgacctgga cttcgcagcg
      841 gttggcgagg ccggcggcga cgacgttctt ggcgacccag cgcacggcgt aggcggcgga
      901 gcggtcgacc ttggtcggat ccttgccgct gaaggcgccg ccgccgtggc gggccatgcc
      961 gccgtaggtg tcgacgatga tcttgcggcc ggtcaggccg gtgtcggcgc aggggccgcc
     1021 cagctcgaag cggccggtgg ggttgacgaa cacccgcagg ccctcggcgc cgaggtcctc
     1081 ggggagggtc gggtcgatca cctgctcgcg gatgtcgggg gtgagcaggg tgtcgaggtc
     1141 gatgtcgggg ccgtgctggg tggagaccac cacggtgtcg aggcggcggg gcctgccgct
     1201 ctcgtattcg acggtgacct gggtcttgcc gtcggggcgg aggtagggga tgtcgccgga
     1261 gcggcggacc tgggcgagcc ggcgcgacag gcggtgggcc agcgcgaccg gcacgggcat
     1321 cagctcgggc gtctcgtcgc aggcgaagcc gaccatcatg ccctggtcgc cggcgccctg
     1381 gacgtcgagc gggtcgacgt cgccgacgcg gtgctcccag gcgtggtcga cgccctgggc
     1441 gatgtcgggc gactgctcgt cgatggcgac ggtgacgccg caggtggacc cgtcgaagcc
     1501 gtatttggcg cggtcgtagc cgatgtcgag gatggtcttt ctgaccacgc tcgggatgtc
     1561 cacatacgtc gaggtggaga tctcgccggc cacgaccacc aggccggtgg tgatcagcgt
     1621 ctcgcaggcg acccggccca tcgggtcgga ggtgaggatc tcgtcgagga ctgcgtcgga
     1681 aatctggtcg gccatcttgt ccgggtgacc ctcagtcacc gactccgacg tgaacaggta
     1741 ccgcttcgcc acgggtgcct cccaggggct tccagggaaa tcttcgcgcc gcgagcaccc
     1801 cgcggcgagc ttcgccgaag gttagtcgtg ctcagacgcc agggcaaatg tacccggagg
     1861 ggcgagtcag ccgtcacgag ctgccaggct ggtcacgtgt ccgcaggtgg acggccaact
     1921 cgtccacgac ctgcgccagc tcgccttcgc ggttgtcgac cacatcgtcg tagtagggac
     1981 ggtaggcgtc cactcgagcc gcgttgccga gccgctcggc gatctcgccc tcgttgtgct
     2041 tgccgcgtgc ctccagccgg cggcggagcg tctcaggagt gacgctgagc aggatcgttc
     2101 tggcctctgg aacgacctgc ttgacgaggc ggacgttgcc gaggttgatg atgccgaggg
     2161 catcccggcc ggccagcagc gagtccatca ccggctgcca tggcagccca taggacatct
     2221 ggaactcgaa gtcacggaac tcgatcagct cgccgttcgc gatcagctgg ctgaactgct
     2281 cgccgccgac gaagatgtat tccgattcag taaccgtttg gcgtggcggc cgtgatgtgt
     2341 agcggcggat gaaatccaag ccggggaccc ggtcggcgag gagctgcgcg atggtcgtct
     2401 tgccggcacc gctgggcccg tcgagcacaa acagcgttcc cggaaccacg ccggaagagg
     2461 ccgtctgctg gagtcgctgc cgccagctgc gcccagctgc ctggatggtc gcgaaatccg
     2521 cgtatgtcca ggcaacgacg tcagcgtcgc cgatcccgag cgctgaacgc acctcgctcg
     2581 cgatcgtgtc agcggcctcc tccgactcgc agcgatcggc gagcaccacg acctcgatga
     2641 aggagccgag ttcccgcacc tggtcaaggt ggacaagaac ctgcttgtgg aggaaggttc
     2701 gtcgctgctt ttcgacgacg acacgcacgc cgttcgcggc cgcgaggaca gcttcgagcg
     2761 cgtccgcgcc ggtcgcgggc agctccgcga caatcgctgg ggtgcggtgg atgcctggct
     2821 ggttggcgag ggtgtaggcg atcagccggg cagcctggtg agcggtcctg cggagcagca
     2881 gccgcccgcg gggcacccgc cgaacgtgga agtaggtgtc gcgctcttcc gaggctcccg
     2941 attccatcgc cccgaaatcc ctgacagcct cggcggtagc ctccggatcg ctgcagaacg
     3001 ccttcagctc gattcgatca cccatgccga atgactgttc gtgacgccag cgaccactcc
     3061 ctggcgagca acggcacgac caccacgacc actacgcctc cgtcccatcg tgggtagggg
     3121 gacgccgccg cagcctgaag cggcagccga aatcgctggc agtatacggc agcgtcgcga
     3181 cctcgccgtc ctgttgaggg tcgtggctgg agctcagacg ggaatcggcg tcgcaccctc
     3241 ccgaacccgg ttgtcaggct ggtcggggcg gacgacgctg ggcacgcagg cgctcgacgc
     3301 ggtggaggag gcgccgggcg atctcctgtt tggaagcgcc ggcaggaagg tcctccgcgg
     3361 cagtggcgtc gaccagggcg gcgtcggcgg cgtcggctcc gaaggcggaa cggtcgggct
     3421 cgacatggtt gacaacgacc aggtccaagc gcttgcggtc gagcttggcg cgggcattgg
     3481 cgaggtgatc gtcggtctcg gcggcgaagc cgaccaggac ctggtccggg cggcggcggc
     3541 ggccgagctc ggcgagcgcg tccggggttg gctcaagggc caactccggt acaccctggt
     3601 ccttcttgag cttgacggag gcactgacct tcgggcggaa gtcggcgacg gcggcggcca
     3661 tgatcaacac gtcgtgggcg ggcgcttcgg cgaggacggt gtcgtagagc tgctgggcgg
     3721 tctcgacctg gagcacacgg gtgccagcgg gcaggtcgac gctggtgacg gccgcgacgg
     3781 cggtgacctc ggcaccgcgg cgcagcgcct ccaccgcgat ggcggcgccc atgcggccgg
     3841 aggagcggtt gccgaggtag cggaccgggt cgagcggctc gcgggtgccg ccaaggctga
     3901 tcagcatgcg gacacccgcc agcgtgcgcg cctcggctgg tgaatcacga tccttgagcg
     3961 cgacggcgac cccggcgacc aggtcggaca gttcggcgag gcggcccagc ccgacgtcgc
     4021 cggaggtgag cgggccgctg gcgggcagca cgacctggac gccgcggcgt tccagcacgg
     4081 cgagatttgc ctgggtggcc ggatgccgcc acatctcggt atgcatggcc ggggcgacaa
     4141 ccaccgggcc agggaaggcg agagcgacgt tggtgagcag gtcgtcggcg aagccggcgg
     4201 caagccgggc gagggtgtgc gcggtggccg gcgcgaccat cagcacctgg ccccagcggg
     4261 ccaactccac gtgctcgacg gcggcggggt cgtcccagat ggaggctggg acctggcggc
     4321 cggtgagggc ggcaaaggtg gcacgggtca ccagcgcctc ggcggcggcg gtgagcaacg
     4381 gctgcacctg cgcccctgcg cgcatcaact ctctcgccag ctcagccgcc ttgtaggcgg
     4441 cgatgccgcc gctgaccccg agcaacaccc gggcgccccg aagagtgggg tgccccgcac
     4501 ccgccggggt cccggtcatc gggctacttg atcgcggcgg cggtctcggc tgggcgctcc
     4561 cattcgagct tgccctcgtc gagttcctgc agcgcgacgg agagggcctt gcggttgggg
     4621 ccggcgtcga ccatcggcgg gacgaactcc ccgatgccct cgccgagttg cgagtagtag
     4681 ccgttgatct gcctggcgcg ccgggccgcc aagatcacca gggtgtactt cgagtccacc
     4741 cgtgcgagca ggtcgtcgat cttcggttca atcattggcg ggatcgatct cctcaaaggt
     4801 tttgaggcgt agaacggtca gccccgatgg tacgcccccg accgcacccg gctgaggcgc
     4861 gccgggtgat ggtcgagcgg tgcggtcgcg gtgagcttgt ggcgccgcgg ctacttcttg
     4921 gtgccgaact ccgccagcag ggcgtccttc tgcttggagc cgaggccgcg gacgcggcgg
     4981 gatgcggaga tgtcgtgctt ctccatgatc ttgcgcgccc ggaccttgcc cacgccgggc
     5041 aggctctcga ggacgctggc gaccttcatc ttgccgacgg tgtcgtcgtc ttccttgtcg
     5101 aggacggtcg tgagcgtcag gttgccgctc ttgagctgcg ccttcagctc ggcccgcttg
     5161 cgccgggcct cagcggcctt ctccagggcc tgctggcgct gctcgggcgt cagggttggc
     5221 agtggcatgt tgcctcctcg tgaggtggtg ccctcagagc ggacggtgct gttcgccccg
     5281 cctgccgata cggttcgttc acagatcgcc ggggattcta cgcacaccaa ggcggccttc
     5341 acaatctatc ggcgcaggtc agacaaggat caacccaaaa aaacgctgga tcgcgtgctg
     5401 agttcggtga ctttaggggc tttcgccggt caaatcctgc cccggctccc gggcgtctcg
     5461 gcgttcgcgt cctggaggca acccgggcat gccgcggact cccaggcgcc cgcctcgatt
     5521 cgggcaagcg ccgtgaacgg gaccggcagc cgcgccccgg cggggagccg gtcgatgatc
     5581 gtgaccaacc cggcgaccgc ggcgccggcg gtgcggagca gctcggcgac ctccagcgcg
     5641 gacccgccgg tggtgacgac gttctcggcg atcagcaccc gctcccccgg cccgacggtg
     5701 aacgagcggc gcagcgccat cgcaccgccg gcgccgcggt cgccggtgcg ttcggtgaac
     5761 accatgcgca cgccgagggc gcgggcgact tcgtgcccga tgacgacccc gcccatggcg
     5821 gggccgacga cgacgtcgac ggggccgccc gctgcctggc tgtgccagcg gctggcgagc
     5881 gagcggccga gcgcctcggc ggccggcggc cactgcagca ccggcgccga ctgcaggtag
     5941 gtgtcggagt gcagccccga ggagagcacg aaatggcccc gccgcagccc gccagcgtgc
     6001 tggtagaggg cgagcaccgc gtcggcgtcc aggggtcccg gccccggggg gagcggtggt
     6061 ggcgcgggcg gctggctgcc ggcctggcgc ggggtcatgg acgccccgcc agcgccggca
     6121 tggttcgccg cagcgccccg ctcgccctgg cactcaccgt gccgccccgg cggtctgggt
     6181 ctcggcggcg atcgtggcgg ctgccgcggc cgggtcgggc gcccgggtga tgggccggcc
     6241 gacgaccagg aggtccgcgc cggccgcgag ggccgccgct ggggtggcga cgcgggcctg
     6301 gtcgtcggag tcgggctggg gggccgccgg ccgcacccct gggcagatga tggtgacgtc
     6361 cgggccggtg agggcgcgca ggcgggcgac ctccagcggg gagcagacca gggcctggca
     6421 gcccgcttcg agggccagcc gggtgaggcg cgtggccgcg cgggtggcgt cgccggcgcc
     6481 gagcgcggcg aggtcgtcgg ggccgaggct ggtcaggacg gtgaccgcgg cgatcctcgg
     6541 gccggggccg gggccggccg ccctggccgc ctggacggcg gcggtgagca tggcggtgcc
     6601 gccgccggcg tggacggtca gcagctcggc gccgagcggg acgagggcgg cgacggcgcg
     6661 gccgacggtg gcggggatgt cgtggagctt gaggtcgagc atcacccggt gcccgtcggc
     6721 gcgcagcgtg cggaccgcgt cggggccgtg ggcgccgaac agctccagcc cgaccttgac
     6781 caggccgacc gagccgctgg tggcggcggc cagcgccctg gcctcggcga ggtcgggcgt
     6841 gtcgagcgcg aggacgatcg ccgaccctga ccctgacgct gtgcctgacc ccatcccctg
     6901 accctccgct cgtgcgtcaa cgttcggctt tgaacagcgc cggcagctcc gcgacggtgg
     6961 cgacgccctg ctcggcgagg tagcggcgca gcccgtcggt gatctccagg ggccgccgcg
     7021 ggctgacgaa gttgccggtg ccgaccgcca cggcggtcgc accgaccagc aggaactcgg
     7081 ccgcgtcgtc ggcgctcatg atcccgccgg tgccgacgat cgggacgccg ggcagggcct
     7141 tggcgacctg ccagacgcag cgcaccgcga ccggccggat cgcggggccg gagaggccgc
     7201 cggtgaccgc ggcgagcttt ggccgccgcc ggtgcacgtc cacggccatc cccagcagcg
     7261 tgttgatgag ggtgagcccg tcggcgccgg cgtcgaccac ggcgcgggcg accctgacca
     7321 gatcggtcac gtcggcggtg agcttggcga gcaccggctg gccggcgacg gccttgaccg
     7381 cccggatgac gctggcggcg gcgtcggggt cggtggcgaa cacctcgccg cggtggtcga
     7441 cgttcgggca ggagaggttg acctccagca gggtgacggc gtcctcgccg ctgaactgct
     7501 cggcgacgcg gccgaactcc tcggcgctgc ggccgacgac gctggcgacc acccgcgcgc
     7561 cggcctgggc caggaacggc agctcgctgg cgcggaaggc gtccacgccg gggttctgca
     7621 ggccgatcgc gttgagcatc ccggcgggag tctcggcgat ccgcggggtg gggcggccgg
     7681 ggcggggttc caggctgacc gacttggtga cgaccgcgcc gagctcaccg aggtccatga
     7741 agcgggccat ttcacgaccg aacccgaagc agccggaggc gaccagcacc ggggacggca
     7801 gccggacgcc gcagagctcg acggtgaggt ccgggtcggg gatggtcatc gacatggtca
     7861 catcgcctcc tctgccagcg acgctgggta gccggggccg ccgctcaggc ccgcgctgag
     7921 gccgagctcg gcccaggcga cctgggcgcc gtccaggacc gggccttcca cgcaggagcg
     7981 ggccatgcgg atcccccctt cgggggcggc catggggacc acgcaggagt agcagatgcc
     8041 ggtcccgcag gccatcagct cctcgaccgc gacctgacag gcgaccttgg cggtggtggc
     8101 gacctggctg accgcggcga gcatcgccat cggcccgcag gcgtagacca gctcggtgcg
     8161 ggccgcctcg atcacctccg gcatcgggtc ggtgacgatc ccgcggcgcc cggcggagcc
     8221 gtcgtcggtg gtgatcacgg cggagcgggc gaggcgtttg gcctccatcg ccttgaacag
     8281 ccgctcctcg gtggaggcgc cgacgatgaa gtcgatccgg cagccacgcg cgagcagccg
     8341 ctcggccagg aagaacagcg gggcgctgcc gtagccgccg ccgatcagca ggcaggaggt
     8401 gggctgctcg ggcagggtga aggcatggcc gagcgggccg accaggtcga cggtgtcgtg
     8461 cgggcgcagc cacgacagcg cgcgggtgcc cggaccgacc acgtcgaaga cgacctccag
     8521 cgagccggac ccggcgccgc gccagtcgac ccggtggatc gagaacggcc gccgcagcag
     8581 gtacgcccgt tccgggccgg tgagcagccc gatgaactgc cctggctggg cggtgcgggc
     8641 gacctcggag gcggccaggg tgagggtgtg gtagacgccc gcccgccggt ggccgagcac
     8701 ctcagccacc tgaagactgg ggggtgcggg gccccccgat gggacggggc ggccatcctc
     8761 aggcattacg catccccttc gctccccctc tggtggtagg cctgcaggga ctgcaccgtc
     8821 agctccccgg ccaggcgggc ctcgatgccc tggaccatgg cggcgaggcc ggagagtgtg
     8881 gtgacgcagg ggatatcggc tgctacggcg gcggtcctga tctcgtatcc gtcggtgcgc
     8941 ggcccggagc cgtggggggt gttgaccacc acgtcgatct cgccgccgcg gatcaggtcg
     9001 acgacgttgc cggggccctc gtggaccttt cggacgaccc gggcggggac gccgttgcgg
     9061 ttcaggacgc tggcggtccc ggcggtcgcg accagggtga agccgaggtc ggcgaggcgc
     9121 ttggcgggca ggacgatggc gcgcttgtcg gcgttgcgca ccgacaggaa cgccacccct
     9181 ttcgtcggca acgccccgta ggaggcctcc tggctcttgg cgaacgccgc cccgaagctt
     9241 tgatcgaggc cgagcacctc gccggtggag cgcatctccg ggccgagcag cgaatcggcc
     9301 cccgggaagc gcgcgaacgg cagcaccgcc tccttcaccc cgacatggcg cggttcaggg
     9361 gcgaggatcc cctcccccag cgggagcatt cccccgggct ggcgcagctc cgggaggcgc
     9421 gcgccggcca tcagcagcgc ggcggccttg gcgaccggca ggccggtgac cttctcgaca
     9481 aacgggaccg aacggctggc acgagggttg gcctccagga cgtagagccg ctcggccttg
     9541 aaggcgaact ggacgttgag gatgccgacc actccgaggg cgcgggcgag cttggcggtg
     9601 gcggcggcga cctcggcgac ctggtcggtg ccaagcgtga tcgccggcag cacgcaggcg
     9661 gagtcgcccg aatgcacccc cgcctcctcg atgtgctcca tcaccccgca gaccaggacg
     9721 ttcccgtcgg cgtcgcagag ggcgtccacg tcgacctcga ccgcatcctc aaggaaccgg
     9781 tcgatcagca ccggcctcga cccgtggtcc atggtcgcca gcgcggccga gcgggtcagc
     9841 gcctcggcat cgtaggagac ctccatggag cgcccgccga gcacgtagga gggccgcgcc
     9901 agcaccgggt agccgatccg ctcggcgacc gccagggcct cctcgcggct ggccgcctca
     9961 ccccaggcgg gcgcgtccag gcccagctcg gccaggacgg tgccgaagcg gccacggtcc
    10021 tcggcca
//
LOCUS       DASWDC010000280         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_579066, whole genome shotgun sequence.
ACCESSION   DASWDC010000280 DASWDC010000000
VERSION     DASWDC010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579066"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1146
                     /locus_tag="VF995_09050"
     CDS             172..1146
                     /locus_tag="VF995_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9343746.1"
                     /translation="MAKASSRHATVRIGTAGWTDPTLIQSRRFYPPGTNSAEARLRYY
                     ASQFPIVEVDASYYYLPTEQQAGLWVERTPPGFVFNVKAFSLLTGHPTRRNALPKDLD
                     GELDPGKRDAQRLSANHLSADALTEVWRRFHEALLPLDSAGKLGAVLLQFPEWFGPRR
                     SSREELRRVRERLPGYQVCIEFRNRAWLEEGTSRDRTLSFLAEEHLPLVCVDMPQGFR
                     SSMPPLAEATSPDLAVVRFHGRDPKAWEHKGPTAGPRFRYLYTEDELEEWVPRVEQLA
                     EHTRDVHVIMNNCVEDYAVRNGRQLAGLLEQDADAPPGMLVQPGLTSS"
     gene            complement(1147..1995)
                     /locus_tag="VF995_09055"
     CDS             complement(1147..1995)
                     /locus_tag="VF995_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343747.1"
                     /translation="MRYRYRQPLTPVTAALAAAVALMLAGCAPAGAIRTSAVGGTTDP
                     TTAVVNAAQATARAGSARIAATFQNGDSGQALTTTGLIDFQRHLVRLSSSLLQSQNGQ
                     TTITLLADGVLYQQLPPSFADRLGRHWVKLDLSGLGAAAQPNVQDVSHTLELLTGAAN
                     FRQIGSDTIRVGTATHYQGTVDRAKLDDSNLAPQLRQWLDRSGATTIPSDAWVDQQGR
                     LAKLRYTLNLANPSTQTRVATVVTTLEFWDFGTPVDVQLPAPGDTTDLGTLKGTVQVQ
                     PQPQTS"
     gene            complement(2063..>2947)
                     /locus_tag="VF995_09060"
     CDS             complement(2063..>2947)
                     /locus_tag="VF995_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019544191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX9343748.1"
                     /translation="RVTRLVLDSPSLVYRAFFALPKSIRDPAGNSVNAAHGYLDMLSR
                     LLVDRRPAGVVNTFDADWRPQWRVDAYPGYKAQRPEDPPELPGQFPVIRQVLDAAGLP
                     VAEAPGYEADDVIGTLAAQAGAGERVAIVTGDRDLFQLVRDPVVRVLYPLRGTSQLAL
                     MDEAGVTEKCGVPPSRYADFAILRGDPSDGLPGISGIGAKTAARLVNEYPTLAALVQA
                     AGTLGPRLATALTQSRDYLAAMDRVVPVVTDLEYQITPPGPPDRDRLDALGEQHRLAG
                     PIRRLLAAMDTAGLARVS"
BASE COUNT          383 a          983 c         1155 g          426 t
ORIGIN      
        1 tggccgcggt cggactgctg accgccggct cgctccccgc ggcccgcgcg gtgctcaccg
       61 gtcccaccgg ggccacccgt cctccgtgac gagtgacgat cctgaaggcc ggaccgtccg
      121 ccgtcaggtc ggccggcagc caccgtcacg gccctccgct accgtggatg catggcgaag
      181 gccagcagca ggcacgccac cgtccggatt gggacggcgg ggtggaccga cccgaccctg
      241 attcaatcca ggcggttcta cccgccgggg acgaactcgg ccgaggcgcg gctgcgctac
      301 tacgcctcgc aattcccgat cgtggaggtc gacgcctcct actactacct cccgaccgag
      361 cagcaggcag gcctgtgggt ggagcggacc ccgccgggct tcgtcttcaa cgtgaaagcc
      421 ttcagcctgc tcaccggcca cccgacccgg cgcaacgcgc tgccgaagga cctcgacggc
      481 gagctggacc ccggcaaacg cgacgcgcag cgcctctccg ccaaccacct gtccgccgac
      541 gccctcaccg aggtctggcg gcgcttccac gaggcgctgc tgccgctcga ctcggccggc
      601 aagctcggcg cggtgctcct gcaatttccg gaatggttcg ggccgcgccg ctccagccgc
      661 gaggagctgc ggcgggtccg cgagcggctc cccggctacc aggtctgcat cgagttccgc
      721 aaccgcgcct ggctggagga gggcaccagc cgcgaccgca cgctcagctt cctcgccgag
      781 gagcaccttc cgctggtctg cgtggacatg ccccagggct tccgttcctc gatgccgccg
      841 cttgccgagg cgacctcccc tgacctggcg gtggtccgtt tccacggccg cgaccccaag
      901 gcctgggagc acaagggccc caccgccggc ccgcgcttcc gctacctcta cacggaagac
      961 gaactggagg agtgggtccc gcgggtcgag cagctcgccg agcacacccg cgacgtccac
     1021 gtgatcatga acaactgcgt cgaggactac gcggtccgca acgggaggca gctcgccggg
     1081 ctcttggagc aggacgccga cgcgccgccg ggcatgctgg tgcagccggg gctgaccagc
     1141 tcctgatcag ctggtctggg gttgtggctg gacctggacg gtgcctttga gggtgccgag
     1201 gtcggtggtg tcgcccgggg cggggagctg gacgtcgacg ggggtgccga agtcccagaa
     1261 ttcgagggtg gtgacgacgg tcgcgaccct ggtctgggtc gaggggttgg cgaggttcag
     1321 ggtgtagcgg agtttggcga ggcggccttg ctggtcgacc caggcgtcgc tggggatggt
     1381 ggtcgcgccg gagcggtcga gccactggcg gagctggggt gcgaggttgc tgtcgtcgag
     1441 cttggcgcgg tcgacggtgc cctggtagtg ggtggcggtg ccgacgcgga tggtgtcgga
     1501 cccgatctgg cggaagttgg cggcgccggt gagcagctcc agggtgtggc tgacgtcctg
     1561 gacgttcggc tgggcggcgg cgccgaggcc gctcaggtcg agtttgaccc agtggcggcc
     1621 gaggcggtcg gcgaacgacg gcggcagctg ctggtagagg accccgtcgg cgagcagagt
     1681 gatggttgtc tggccgttct ggctctgcag caggctgctg gacagtcgca ccaggtgacg
     1741 ctggaagtcg atcaggccgg tggtggtgag ggcctggccg ctgtcgccgt tctggaaggt
     1801 ggcggcgatg cgggcgctgc ccgcccgcgc ggtcgcctgg gcggcgttga cgaccgccgt
     1861 ggtcgggtcg gtggtgccgc cgacggcgct ggtgcggatg gcaccggcgg gcgcgcagcc
     1921 agccagcatg agggcgacgg ctgcggcgag ggcggcggtg acgggcgtga gcggttggcg
     1981 gtagcggtag cgcatgcgct cccccagctt cgggaagacc ggtgtgctgc ggtgtccgcc
     2041 tggttctgcc ccgacgaggc gctcaggaaa cccgggcgag gccggcggtg tccatggcgg
     2101 ccagcaggcg gcggatcggc ccggcgaggc ggtgctgctc gccgagagcg tccaggcggt
     2161 cgcggtcggg cggcccgggt ggggtgatct ggtactcgag gtcggtgacg accgggacga
     2221 cgcggtccat cgcggcgagg tagtcgcggc tctgggtcag cgcggtggcg aggcgcgggc
     2281 cgagggtacc ggcggcctgc accaacgccg ccagggtcgg gtactcgttc accaggcggg
     2341 ctgctgtctt ggcgccgatg ccgctgatgc cgggcaggcc gtcggagggg tcgccgcgca
     2401 ggatcgcgaa gtcggcgtag cggctcggcg gcaccccgca cttctcggtc acccctgcct
     2461 cgtccatgag ggcgagctgc gaggtgcccc gcagcgggta caacacgcgg accaccgggt
     2521 cgcggaccag ctggaacaga tcacggtcgc cggtgacgat cgcgacccgc tcgccggcgc
     2581 cggcctgggc ggcgagcgtg ccgatgacgt cgtcggcctc gtagccgggc gcctccgcca
     2641 ccggcagccc ggcggcgtcg aggacctggc ggatcaccgg gaactggccg ggcagctcgg
     2701 gcgggtcctc gggacgctgg gccttgtagc cggggtaggc gtccacgcgc cactggggcc
     2761 gccagtcggc gtcgaaggtg ttgaccaccc cggcgggccg ccggtcgacc agcagccggg
     2821 agagcatgtc gaggtagccg tgggcggcgt tcaccgagtt gccggcgggg tcgcggatgc
     2881 tcttcggcag cgcgaagaag gcccggtaca ccaggctggg cgagtcgagc accaggcggg
     2941 tcacccg
//
LOCUS       DASWDC010000281         5100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_734561, whole genome shotgun sequence.
ACCESSION   DASWDC010000281 DASWDC010000000
VERSION     DASWDC010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734561"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VF995_09065"
     CDS             <1..530
                     /locus_tag="VF995_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343749.1"
                     /translation="LFLSSTRVIFAAAFDRILPERVADVSERRHVPVYALVLMLVPSI
                     VVSAFYSYTAQMKTYILDAVLVIAVTFLGTAIAAMLLPYRRKQLFENSAIARYKVFGV
                     PLITIAGAITAAFLAFNLWKWLKDSLYAVNNKTSLWFMGGMYLLALVVYVVAKLVRRS
                     QGIDLKAIHQEIPVE"
     gene            568..3006
                     /locus_tag="VF995_09070"
     CDS             568..3006
                     /locus_tag="VF995_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9343750.1"
                     /translation="MIVGAGIVGASAAWHLAELGQPDVLVLDRGPLFETGGSTSHAPG
                     LVFQTNPSRLMCRVAQDTVALYKTLELDGEPGWYGVGSLEVATTPERAWELKRRLGWA
                     RSYGIEGGELLSPEQAAERVPLLDPATILGAYHVPSDGIAKAVRIVAALARAASAKGV
                     VFKGGVTVTGFDLHDGRVRAVETDQGRVECERVLICAGVWGPTVGALAGVPVPLVAVE
                     HQLVWTDPLPELAGETREVAHPIVRHQDMAMYFRQREDHYGVGSYQHEPIAVPQRSLG
                     GDAVNPFTPGDFDIAEAEAARVMPALKGTMRPEDPARSLNGIFSFTPDAASIVGESAR
                     ARGVWVCEAVWITHAGGMGRQVAEWMALGEPSYDLAEADANRFHGFQTAPPYLLERGK
                     QQYREVYDIIHPRQQPARPRELRLTPFYRRQVDLGARFVTAAGWERPQWFEANGTLLS
                     EAGAGGDALWGRRDGWAAKLWSPVEGAEHRATRSHAALFDLTPFAKADVAGPDALAFL
                     ERICGNRIDRPVGSVVYTAMLTPRGRIRCDLTVTCLAHDRFRIITGGGSGQHDLAWIR
                     AQRRPGERAAVTEWTSELFTLGLWGPKARAILAEVSDADVSNGAFPYLTGRWLHVGEV
                     PVWAQRISYAGELGWELYGEFAMGDRLWELLWEAGREHGLIAAGLGAFDTLRLEKGYR
                     LWGQDIDAEHDPLEAGLGFALRWDKDFQGREALLEARQRGLRRKLVPMVFDDLDVVVL
                     GKEPIRVDGRVVSSVTSAGYGYSIDRGIVYGYLPAELAAVGTRVEVEYFDQRHPATVA
                     SEPLFDPKGERLRA"
     gene            3038..3778
                     /locus_tag="VF995_09075"
     CDS             3038..3778
                     /locus_tag="VF995_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020058300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEX9343751.1"
                     /translation="MAEEQEQEQEQEQEQEEIVLVELPDEELVKQIHDDLYDGLADEI
                     VEGTSILLDRGWGPDQVLNQALVEGMRIVGIDFRDGILFVPEVLLAANAMKAGMAILR
                     PLLAETGAERVGKVVIGTVKGDIHDIGKNLVAMMLEGAGFEVVDLGINNPVERYLEAL
                     EKHKPDILGMSALLTTTMPYMKVVIDELVAKGIRDDYIVLVGGAPLNEEFGRAVGADG
                     YCRDAAIAAEMAQAMVLERRAQRQGASR"
     gene            3775..4422
                     /locus_tag="VF995_09080"
     CDS             3775..4422
                     /locus_tag="VF995_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEX9343752.1"
                     /translation="MTALQPRPRLLVIACGAIAREVLAVLRLNGWDREVTLRCLPAQL
                     HNTPQRIAARVDAKLHEAEAEGYPPTQILVAYGDCGTGGALDRVLDEHGVGRLPGDHC
                     YAFFAGVDSWLAMHDAEPRTFYLTDFLCRHFDRLVVRGLGLDRHPELLPAYFGNYLRL
                     VYLAQRADEALLANAREAAAYLGLTLEVRHSGYGDLAPALQAARRRAVEVEVACA"
     gene            4419..>5100
                     /locus_tag="VF995_09085"
     CDS             4419..>5100
                     /locus_tag="VF995_09085"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9343753.1"
                     /translation="MTHTVVSSASKEVVIGFDRPFVIIGERINPTGRKRLAAEMALGD
                     FSTVIADALAQVEAGAHMLDVNAGIPLADEPAILTRVVELVQSVTDVPLCIDSSIVEA
                     LEAALPAYKGKALVNSVTGEEEQLERVLPLVARHGAAVVAISNDERGISENPDVRFEV
                     ARKIVERAADYGITPADIVVDPLVMPIGAMASAGRQVFRLVRRLRQELHVNTTCGASN
                     IAFGLPNRP"
BASE COUNT          754 a         1770 c         1835 g          741 t
ORIGIN      
        1 cgctgttcct gtcgtcgacc cgggtgatct tcgcggcggc gttcgaccgg atcctgccgg
       61 agcgggtggc cgacgtgtcg gagcggcggc acgtcccggt gtacgcgctg gtgctgatgc
      121 tggtcccgtc gatcgtggtg agcgcgttct actcctacac cgcgcagatg aagacctaca
      181 tcctggacgc ggtgctggtg atcgccgtca ccttcctcgg cacggcgatc gcagccatgc
      241 tgctgcccta ccgccgcaag cagctgttcg agaactcggc gatcgcccgc tacaaggtgt
      301 tcggcgtgcc gctgatcacg atcgcgggag ccataaccgc cgcgttcctg gccttcaacc
      361 tgtggaagtg gctcaaggac tccctctacg cggtcaacaa caagacctcg ctgtggttca
      421 tgggcgggat gtacctgctc gcgctggtgg tctacgtggt cgccaaactg gtgcgccgca
      481 gccaggggat cgacctgaag gcgatccacc aggagatccc ggttgagtag cgcaccgccc
      541 acagcatttc cggagcgagc ccagaccgtg atcgtcggtg ccggcatcgt cggcgccagc
      601 gccgcctggc acctggcgga gctgggtcag ccggacgtgc tggtgctgga ccggggcccc
      661 ctgttcgaga ccggcggctc cacctcccac gcgcccggcc tggtgttcca gaccaacccg
      721 tcgcgcctga tgtgccgggt cgcgcaggac accgtcgcgc tctacaagac cctggagctg
      781 gacggcgagc cgggctggta cggcgtcggc tcgctggagg tcgccaccac cccggagcgg
      841 gcctgggagc tgaagcgccg cctcggctgg gcccgctcct acggcatcga aggcggcgag
      901 ctgctcagcc ccgagcaggc cgccgagcgg gtgccgctgc tggacccggc cacgatcctc
      961 ggcgcctacc acgtccccag cgacgggatc gccaaggcgg tccggatcgt ggccgcgctc
     1021 gcccgcgccg ccagcgccaa gggggtcgtg ttcaagggcg gtgtcaccgt caccgggttc
     1081 gacctgcacg atggccgggt gcgggcggtc gagaccgacc agggccgggt ggagtgcgag
     1141 cgggtgttga tctgtgcggg cgtctggggc ccgaccgtgg gcgcgctggc cggggtgccg
     1201 gtcccgctgg tcgcggtgga gcaccagctg gtctggaccg acccgctgcc ggagctggcc
     1261 ggcgagaccc gggaggtcgc ccacccgatc gtgcgccacc aggacatggc gatgtacttc
     1321 cgccagcgcg aggaccacta cggcgtcggc agctaccagc acgagccgat cgcggtcccg
     1381 cagcgcagcc tgggcggcga tgcggtcaac ccgttcaccc ccggcgactt cgacatcgcc
     1441 gaggccgagg ccgcgcgggt catgccggcg ctgaagggga ccatgcgccc cgaggacccg
     1501 gcccgctcgc tcaacgggat cttctccttc acccccgacg ccgcctcgat cgtcggggag
     1561 agcgcgcggg cccgcggtgt ctgggtctgc gaggcggtct ggatcaccca cgccgggggg
     1621 atgggccgcc aggtggccga gtggatggcc ctgggggagc cgagctacga cctggccgag
     1681 gccgacgcca accgcttcca tgggttccag accgctccgc cgtacctgct ggagcgcggc
     1741 aagcagcagt accgcgaggt ctacgacatc atccacccgc gccagcagcc ggcacggccg
     1801 cgcgaactcc gcctcacccc gttctaccgc cggcaggtcg acttgggcgc gcggtttgtg
     1861 accgccgccg ggtgggagcg cccgcagtgg ttcgaggcga acgggacgct cctcagcgag
     1921 gccggggccg gcggtgacgc gctctggggg cgccgagacg gctgggcggc caagctctgg
     1981 tcgccggtcg agggcgccga gcaccgcgcg acccggtccc atgccgccct gttcgacctc
     2041 acccccttcg ccaaagccga cgtggcaggg ccggacgcgc tcgccttcct ggagcggatc
     2101 tgcggcaacc gcatcgaccg gccggtcggc tcggtggtct ataccgccat gctcaccccg
     2161 cgtggccgga tccgctgcga cctcaccgtc acctgcctgg cccacgaccg gttccggatc
     2221 atcaccggag gcggcagcgg ccagcacgac ctcgcctgga tacgggcgca gcgccgcccc
     2281 ggcgagcggg cggcggtcac cgagtggacc tcggagctgt tcaccctcgg cctgtggggc
     2341 ccgaaggccc gcgcgatcct ggccgaggtc agcgacgcgg acgtctccaa cggggcgttc
     2401 ccctacctga ccggccgctg gctccacgtc ggcgaggtgc cggtgtgggc gcaacggatc
     2461 agctacgccg gggagctggg ctgggagctc tacggcgagt tcgccatggg cgaccggctc
     2521 tgggagctgc tgtgggaggc cgggcgcgag cacggcctga tcgccgcggg gctgggcgcc
     2581 ttcgacacgc tcaggctgga gaagggctac cgcctctggg gccaggacat cgacgccgag
     2641 cacgacccgc tggaggccgg gttggggttc gcgttgcggt gggacaagga cttccagggc
     2701 agggaggcgc ttctggaggc gaggcagcgc gggctgcgcc gcaagctcgt gccgatggtc
     2761 ttcgacgacc tggacgtggt ggtcctcggc aaggagccga tccgggtgga cggccgggtg
     2821 gtctcgtcgg tgacctcggc cggctacggc tactcgatcg accgggggat cgtctacggc
     2881 tacctcccgg ccgagctggc cgcggtcggc accagggtcg aggtcgagta cttcgaccag
     2941 cgccacccgg cgacggtcgc cagcgagccg ctgttcgacc cgaaggggga gcgtctcagg
     3001 gcctgacagg cagtcgatga accaggggtt tgcgctgatg gccgaagaac aggaacagga
     3061 acaggagcag gaacaggagc aggaagagat cgtcctggtc gagctgcccg acgaggagct
     3121 ggtcaagcag atacacgacg acctctacga cggcctggcc gacgagatcg tcgagggcac
     3181 cagcatcctc ctcgaccggg gctgggggcc agaccaggtc ctcaaccagg cgctggtcga
     3241 ggggatgcgg atcgtcggca tcgacttccg cgacggcatc ctgttcgtcc ccgaggtgct
     3301 gctggccgcc aacgcgatga aggcgggcat ggcgatcctg cgcccgctgc tggccgagac
     3361 cggcgccgag cgggtcggca aggtggtgat cggcaccgtc aagggcgaca tccacgacat
     3421 cggtaagaac ctggtcgcga tgatgctgga gggcgccggc ttcgaggtgg tcgacctggg
     3481 catcaacaac ccggtcgagc gctacctgga ggccctggag aagcacaagc ccgacatcct
     3541 gggaatgtcg gcgctgctca ccaccaccat gccctacatg aaagtggtga tcgacgagct
     3601 ggtggcgaag ggcatccgcg acgactacat cgtcctggtc ggcggcgccc ccctcaacga
     3661 ggagttcggc agggcggtcg gcgccgacgg ctactgccgc gacgccgcca tcgccgcgga
     3721 gatggcccag gcgatggtgc tggaacggcg cgcacagcgg cagggagcga gccggtgacg
     3781 gccctccagc cgaggccacg actgctggtg atcgcctgtg gcgcgatcgc ccgggaggtc
     3841 ctggcggtgc tccgcctcaa cggctgggac cgggaggtga ccctgcggtg cctgcccgcg
     3901 cagctccaca acacgccgca gcggatcgcc gccagagtgg atgccaagct ccacgaggcc
     3961 gaggcggaag gctatccccc tacccagatc ctggtcgctt acggcgactg cggcaccggc
     4021 ggggcgctcg accgcgtgct ggacgagcac ggcgtcggac gccttcccgg cgaccactgc
     4081 tacgccttct tcgcgggggt ggactcctgg ctcgcaatgc atgacgcgga gccgcggacc
     4141 ttctacctga ctgacttcct gtgccgccac ttcgaccggt tggtggtgcg gggcctcggc
     4201 ctcgaccggc atccggagct gctgccggcc tacttcggca actacctgcg cctggtctac
     4261 ctcgcgcaac gcgccgacga agcgctgctc gccaacgcgc gggaggcggc ggcctacctc
     4321 ggcctcaccc tggaggtccg ccacagcggc tacggcgacc tggcgccggc actccaggct
     4381 gcgaggcggc gagccgtgga ggtggaggtg gcgtgcgcat gacgcatacc gtggtgtcgt
     4441 cggcttccaa ggaggtcgtg atcgggttcg accggccgtt cgtgatcatc ggcgagcgga
     4501 tcaacccgac gggacgcaag cgccttgccg ccgagatggc gctcggcgac ttctccaccg
     4561 tgatcgcgga cgccctcgcg caggtcgagg ccggtgccca catgctggac gtcaacgctg
     4621 gcattcccct ggccgacgag cccgcgatcc tcacccgtgt ggtcgagctg gtccagtcgg
     4681 tcaccgacgt gcccctgtgc atcgactctt cgatcgtcga ggcgctggag gcggccctgc
     4741 cggcctacaa gggcaaagcc ctggtcaact ccgtcaccgg cgaggaggag cagctggagc
     4801 gcgtgttgcc gctagttgca aggcacggcg ccgcggtggt ggcgatcagc aacgacgagc
     4861 gcggcatctc cgagaacccc gacgtccgct tcgaggtcgc ccgcaagatc gtggagcgcg
     4921 ccgccgacta cggcatcacg cccgccgaca tcgtggtgga cccactggtg atgccgatcg
     4981 gcgcgatggc cagcgccggg cggcaagtgt ttcgcctggt ccgccgcctg cgccaagagc
     5041 tgcacgtcaa caccacctgc ggtgcctcca acatcgcctt tgggctgccg aaccgccccg
//
LOCUS       DASWDC010000282         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1331271, whole genome shotgun sequence.
ACCESSION   DASWDC010000282 DASWDC010000000
VERSION     DASWDC010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331271"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VF995_09090"
     CDS             <1..558
                     /locus_tag="VF995_09090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9343754.1"
                     /translation="DLLAEAGARATFFCCGEPARLHPELVRRAARLGEVGNHSWSHAD
                     LATLNTAPVGRELDQTHQLLASLTGQPPTLFRPPYGNLRGAVLLVAARHRYGAILWTD
                     MVQHAASTPAADTARLLAHAAPGQILLAHDGRGDRAGILERLPALLHGLRGDGFELTT
                     VSDLLSFDDGLDDGLGGSREGPPGS"
     gene            complement(555..1805)
                     /gene="glgC"
                     /locus_tag="VF995_09095"
     CDS             complement(555..1805)
                     /gene="glgC"
                     /locus_tag="VF995_09095"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX9343755.1"
                     /translation="MTSQYQQPHVLSMVLAGGEGKRLAPLTADRAKPAVPFGGNYRIV
                     DFALSNLVNAGYLRIVVLTQYKSHSLDRHIATTWRMSPLLGNYVTPVPAQMRRGPRWF
                     AGSSDAIFQNLNLIGDERPDYVIVFSADHIYRMDPRQLVEQHIASGAGVTVAGIRVPR
                     SEAHQFGVIQAAADGRGIEAFLEKPEDPPGLPGDPDTVYASMGNYVFTTATLTEVVSL
                     DATDDSSNHDLGGDILPLLVERGEAQVYDFADNEVPGATERDRGYWRDVGTLDSFYDA
                     SLDLISVHPVFNLYNQRWPILTWHDPLPPAKFVFEDGEGRTGQAFDSMVCAGVIVAGG
                     TVRRSILSPGVRVADGALVEGSVLLHGVEVGAHAVVRNAIVDKNVIIPEGARIGTDAE
                     DDRARFAVSPGGVVVIGKGQKVEA"
     gene            1940..2578
                     /locus_tag="VF995_09100"
     CDS             1940..2578
                     /locus_tag="VF995_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343756.1"
                     /translation="MSARTTPPFRADHVGSLLRPPELLRARDEFTAGKIPAEELRTIE
                     DEAIEAVVRMQRDAGLRSATDGEFRRAAWHMDFIYQLGGISRAPEELKIHFRNEQGDL
                     EFTTAALQVDGRIHLEHTIFGPDFQFLQGLVAGTGVTPKLTIPSPNMVHYRGGRATID
                     QRVYPEMEEFWADLAAAYAEEVRRLGELGCTCLQFDDSTSGEAPATRGKPDE"
BASE COUNT          411 a         1030 c          903 g          379 t
ORIGIN      
        1 gacctgctcg ccgaggccgg cgcccgcgcg accttcttct gctgcggcga gcccgcccgc
       61 ctccaccccg agctggtccg ccgcgccgca cggctcggcg aggtcggcaa ccactcctgg
      121 agccacgccg acctcgccac cctgaacacc gccccggtcg gccgcgagct cgaccagacc
      181 caccagctcc tcgccagcct caccgggcag ccgccgaccc tgttccggcc gccctacggc
      241 aacctccgcg gcgccgtcct gctcgtcgcc gcccgccacc gctacggagc catcctctgg
      301 accgacatgg tccagcacgc cgccagcacc ccggccgccg acacggcccg gctgctcgcc
      361 cacgccgcgc ccggccagat cctgctcgcc cacgacggcc gcggcgaccg cgccgggatc
      421 ctcgagcgcc tccccgccct cctccacggc ctgcgcgggg acggcttcga gctgaccacc
      481 gtcagcgacc tgctcagctt cgacgacggc ctcgacgacg gcctcggcgg cagccgcgag
      541 gggccgcccg gaagctaggc ctccaccttc tggcccttgc cgatcaccac gacaccgccg
      601 ggggagaccg cgaagcgggc gcggtcgtcc tcggcgtcgg taccgatccg ggcgccctcc
      661 gggatgatca cgttcttgtc gacgatggcg ttgcggacca ccgcgtgggc gccgacctcg
      721 acgccatgca gcagcaccga gccctccacc agagcgccgt cggcgacgcg cacgccgggg
      781 gacaggatcg agcggcgcac cgtcccaccc gcgacgatca ctcccgcgca caccatcgag
      841 tcgaacgcct gcccggtgcg gccctcgccg tcctcgaaca cgaacttcgc cggcggcagc
      901 gggtcgtgcc aggtcaggat cggccagcgc tggttgtaca ggttgaacac cggatggacc
      961 gagatcaggt ccaggctggc gtcgtagaag ctgtccagcg tcccgacgtc ccgccagtag
     1021 ccgcggtccc gctcggtcgc gccggggacc tcgttgtcgg cgaagtcgta gacctgcgcc
     1081 tcgccgcgct ccaccagcag cggcaggatg tcgccgccga ggtcgtggtt ggaggagtcg
     1141 tcggtggcgt ccagggaaac gacttcggtc agggtggcgg tcgtgaagac gtaattgccc
     1201 atcgaggcgt agacggtgtc cgggtcgccg ggcagccccg gcgggtcctc cggcttctcc
     1261 aggaacgcct cgatgccgcg cccgtcggcc gccgcctgga tgacgccgaa ctggtgcgcc
     1321 tccgagcgcg gcacccggat ccccgccacc gtcaccccgg cgccgctggc gatgtgctgc
     1381 tcgaccagct gccgcgggtc catgcggtag atgtggtcgg cgctgaacac gatcacgtag
     1441 tcgggccgct cgtcgccgat caggttgagg ttctggaaga tcgcgtccga ggagcccgcg
     1501 aaccagcgcg gcccccgccg catctgcgcc ggcaccgggg tgacgtagtt gccgagcagc
     1561 ggcgacatcc gccaggtggt ggcgatgtgc cggtcgaggg aatggctctt gtactgcgtc
     1621 agcaccacga tccgcaggta gcccgcgttg accaggttgg acaacgcgaa gtcgacgatg
     1681 cggtagttcc cgccgaacgg caccgccggc ttggcgcggt cggcggtgag cggcgccagc
     1741 cgcttgccct cgccgccggc gaggaccatc gagaggacgt gcggctgctg atactgggac
     1801 gtcacagcgt gagcgtatcc accccgccct gctttgatcc accatttgga ggatccttgc
     1861 ggccgtgcac catactggtt gaccggtgga ggcgcggcct ccagcctcca ggctgtccgc
     1921 tcgcgcaaag gagggcccga tgtccgctcg cacgacgccg ccgttccgcg ccgaccacgt
     1981 cggcagcctg ctgcggcctc cggagctgct gcgggcacgc gacgagttca ccgccgggaa
     2041 gatccccgcc gaggagctgc gcacgatcga ggacgaggcg atcgaggcgg tcgtgcggat
     2101 gcagcgcgac gccggcctgc ggtcagccac cgacggggag ttccgccgcg ccgcctggca
     2161 catggacttc atctaccagc tcggcggcat cagcagggcc cccgaggagc tcaagatcca
     2221 cttccgcaac gagcagggcg acctggagtt caccaccgcc gccctgcagg tcgacggcag
     2281 gatccatctg gagcacacca tcttcggccc ggacttccag ttcctccagg gtctggtcgc
     2341 ggggaccggg gtgacgccga agctgaccat cccctcgccc aacatggtcc actaccgcgg
     2401 cggccgggca acgatcgacc agcgcgtcta cccggagatg gaggagttct gggcggatct
     2461 cgccgccgcc tatgccgagg aggtccgacg cctcggcgag ctgggctgca cctgcctcca
     2521 gttcgacgat tccacttctg gcgaggcacc tgcaaccagg ggcaaaccag acgaatgaca
     2581 caggctgtgg cgacaccgcc tcgagtgggt ccggacggcc acggccccca cgcctcgccg
     2641 caccaagaca actactctca cctgctggat gatcggacag ggaggtccgc cagttgaccg
     2701 acggatgcag ctatgattct ctg
//
LOCUS       DASWDC010000283        16466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1497503, whole genome shotgun sequence.
ACCESSION   DASWDC010000283 DASWDC010000000
VERSION     DASWDC010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497503"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VF995_09105"
     CDS             complement(<1..262)
                     /locus_tag="VF995_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343757.1"
                     /translation="MASSAGKPGDAAQRSERLLLGTFAFVRLAIYTQTVTAGILVWRH
                     FRAPVVVALVLVACLVESAVVVEVGRRRGALDSGALAVLDVAV"
     gene            complement(333..1052)
                     /locus_tag="VF995_09110"
     CDS             complement(333..1052)
                     /locus_tag="VF995_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9343758.1"
                     /translation="MRPIRIAIVDDHPVARWGVEHIFEAHPEIRVLCSVASVEELEVA
                     TGAGEAAPEVVIVDLYLAGEAPSLAAIERLAARCRVLVMSASGRREDVLAAIRSGADG
                     YLTKQASDDAFLAGVERVAAGQLYLSSQLADMLHADLDLDSALEAGVGAWGGGEQRLA
                     PREEETLRYIARGFTHAQAARRMGVSQTTVETYIKRIRQKLRVGNKADLTRKAIELGK
                     VSAASPPQPPALLADRCDPSD"
     gene            complement(1219..2268)
                     /locus_tag="VF995_09115"
     CDS             complement(1219..2268)
                     /locus_tag="VF995_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX9343759.1"
                     /translation="MGQRRLLAADGGGSKTDLVLLDEDGAVLAAVRGPGSNPDNVGAA
                     RCVAILDALVTRAALVAGLDGQPRPIAELGAYYLSGLDFPAQERDLGARVAACRWSAA
                     SVVGNDCFAALRAGASRGWGVAVVCGTGINCVAVSPSGRHARYPALGPLTGDWGGAAD
                     VGMAALAAAIRSQDGRGPRTSLQRSVAAHFGLRQPQAAGIAIFQGRLPERALEGLPPL
                     LFAAARDGDGVAGGIVDRLADEVVAMAAALLRRLRLTRSDAEIVLGGGILQAGDDRLT
                     ARVHRGIQAVAPHAQPITLALPPVTGAALLALEAAGASPAATSRARAQVPAALRDPIP
                     NPVPDPTRNAGRAGR"
     gene            complement(2271..3638)
                     /locus_tag="VF995_09120"
     CDS             complement(2271..3638)
                     /locus_tag="VF995_09120"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEX9343760.1"
                     /translation="MKVAVVGGGSTYTPELVDGFARRQRQGRLAVDELALHDLDAGRL
                     EVVGGLARRILARQGWPGRLTLTTDLAGAAAGADVAIIQLRVGGQAARQVDETLPLRF
                     GCIGQETTGPGGFAKALRTVPVMLELADRIAALAAPGAWIVDFTNPVGIVTRALLDAG
                     HRVIGLCNVAIGFQRTIAALCQVEPERIQLEHVGLNHLSWVRAAHLDGVDRLPELLAR
                     PDAAQVLAGQAGQPVALLRTLGAVPSYYLRFFYLREQVLAEQRAAPSRAERVAAIEQE
                     LLEAYRDPALAAKPALLEQRGGAFYSEAAAQLVESLVGGRGDVQVVNLRNGTTLAGLP
                     PEAVVEVPARIDRDGAHPLPQRPLAPELLGLVQQVAAYERLAVEAARSGDRRVALRAL
                     LANPLVAQYQVAVPLLEALLEANRAHLPRFFPDPATGARAAAGTAAAGTAAGAQTRTE
                     TETEA"
     gene            complement(3661..4542)
                     /locus_tag="VF995_09125"
     CDS             complement(3661..4542)
                     /locus_tag="VF995_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9343761.1"
                     /translation="MSAAVPALDRPRPAVPPAVRRHRFLVAVATHSGLIACSVLFLAP
                     FAFIVLTALMNDLQAGGQNLWPHPFRWGNFATVIHSFPVLRYAANTFLYAGLATIGVL
                     LSSVPVAYALAKLRWRLRGFFFVLVLATMMLPYQVTAVSLYVIFTRVHWIGTLLPLIV
                     PNFFGDAFSIFLLRQFFMTIPEDYLDAARVDGGSDFQILRRVVVPMARPALAAVALFN
                     FLYAWNDFFGPLLYVINNQANWTLSVALTQFRTLHRVEWNLTMAAVVLVMLPVIALFF
                     FAQRAFIEGVTLTGVKG"
     gene            complement(4539..5426)
                     /locus_tag="VF995_09130"
     CDS             complement(4539..5426)
                     /locus_tag="VF995_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9343762.1"
                     /translation="MSPWLLGFSMFFVYPMAASLWFSFTHYDLLGQPRFIGLANYRYM
                     FTKDQNFWLAVRNTVWVILVAVPARVVFAVGAAMLLTKARRAAGLFRTIFYLPVLAPP
                     VAATLGFVFLFNPTTGPVNHILHLLHLPQPLWFSDPRFAKPALVLLALWGIGDIMIIF
                     LASLLDVPVQLYEAADIEGANAWQKARKVTLPMISPVILFALIIGIIDGLQYFTQGYV
                     ASVAASGSFQVGQSITLGYPQGSTLFFSVWLYQNGFEYFRMGYASALAWTLFLVTLIF
                     TLLLLRISRRWVHYQGGVR"
     gene            complement(5567..6961)
                     /locus_tag="VF995_09135"
     CDS             complement(5567..6961)
                     /locus_tag="VF995_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019058938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9343763.1"
                     /translation="MRHHGERATGANKVGGACGGRAWIAVLAVVALALAACTAGGGGA
                     KTPTTSKTVDTTPKTITFWEGFSANHEKAAIRAAVAKFHARYPWITVKLTDAITDENI
                     VNAIHGGNPPDAAMSFTTDNVGEFCAANAFQDLGSYAKADGTDLSVIPQAARDYTSYK
                     GKQCTLPMLADTYGLYYNTDMFTKAGLDPAKPPRTMSELVADAEKLTVRNPDGSIKVA
                     GFVPLWGFYEASVAHLGQSWGAQWLDADGKSNISTDPRWAEMAKWQKQLVDWYGYKNL
                     VKFAGQPPDEFSAQNMFESGKLAMNLDGEWRVAFLADEKPNLNYATAPFPAADDQPGQ
                     YGAGFVSGNIIGIPRGAKHPEQAWLLVKFLATDTAAQVGLSNDLRNVPTVTAALSSPD
                     IKPDQHFKTFLDIFGNPKSAAVPPTPVGAALQNNFQDVLVKYQAGNVRDLQAALQGVD
                     KTNDNQLAQQVGAP"
     gene            complement(6958..8271)
                     /locus_tag="VF995_09140"
     CDS             complement(6958..8271)
                     /locus_tag="VF995_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014177599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX9343764.1"
                     /translation="MPVWEPSDQPVEAAAERLPAPVPMAAGTPSLLRAINERGAVELI
                     RRFGPLSRADIARRSGLSKPTVSLALARLEAAGLVREVGRTSGGRGASAVLYDLDPSA
                     GWVLGIDVGRRWVRCAATDITGRVVARRDEHAHIRSGHTLITQIGSLARAVAADAGIE
                     LAAVTWATLGSPGVLGPGGERVILAPNLPGWQQPGVVDEVRAELGLEVEFENDVNLAA
                     IAERWRGLGREVDDFVLLSIGTGIGMGVVLGGQLHRGSAGAAGEVGYLPLPLPPGTGG
                     ARARGRRGGAFEAAAGARGVVALARQCGMTKARNAEEVFAAARRGHPDALAAVNLQAH
                     HLALGIAAVAAVLDPDLVILGGGIGHNSGDLLLEPVTRELRGCGPFRPRVAVSALGAD
                     AVLQGALATALLAAQERVFARTEQAPALTGRPSPARSASAKEASA"
     gene            8624..9658
                     /locus_tag="VF995_09145"
     CDS             8624..9658
                     /locus_tag="VF995_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343765.1"
                     /translation="MKIRYAAAMASVAVAALAAAAGMGPSAKASPTAAGATGGAVPIT
                     AGGRAHMFPRPQVLADVHNGDSRGGSPPLQYHSGPIMKTAVNYIVLWNPGHLQNGTAT
                     GYSSKYTSLLSRWFKDVGGGGLYANNTQYSQGGGVFVQNSAKFGGVFTDTTPFPTGQC
                     SNPDTGTNCITDANLAAEAAAVAKAHSISAGLTKDFFVFTPAGEGSCFDAGCSYPSYT
                     YYCAYHSYTNATFGNSPYLLYANMPYPTDPVGNNCYASYLGQTFPNGDVNADAAINVT
                     SHEQMETVTDPLLSAWYDAAGYEIGDECAWNFGSNNWPSGGNQERNGHPYDLQLEASN
                     HSADCVTSGP"
     gene            9820..10635
                     /locus_tag="VF995_09150"
     CDS             9820..10635
                     /locus_tag="VF995_09150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9343766.1"
                     /translation="MRGEHSEPEPTLDIDTSVAHPARMYDYFLGGKDNFPADREAAEK
                     VLAIVPEGRDMARENRAFLGRAVRFLAGSGIRQFLDIGTGIPTQGNTNAVAHAVAHDA
                     RVAYVDNDPIVLVHARALLAAHDQDRTTVVQADLRQPAELLAHPEVRAMIDFQQPVAI
                     LLVAILHFIRDQEDPAGIVACLKQVMAPGSYLVISHGTQDLDPVMAQRAVRPYNQATA
                     PFILRSRDEIASFFDGLELVDPGLVQLPLWRPDGPVTANLDRIWLYGGIGQQP"
     gene            complement(10687..13731)
                     /locus_tag="VF995_09155"
     CDS             complement(10687..13731)
                     /locus_tag="VF995_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9343767.1"
                     /translation="MIEGVARRVSCLELVGRDAEVARLRQALDGAAAGQPAIVVVAGE
                     AGVGKTRLVAELLHDAAGVGAVALTGGCLDVGEGVLAYAPLVEALRSLARGIDPAELE
                     RALGAARGELTRLVPELAPQDGGEVAPAPGPLVPTRLFELLLGMLHRLAERAPVLLVV
                     EDLHWADQSTRNLLGFLARNLRGGVALVVTYRSDELHRRHPLRPFLAELDRSGRAERL
                     ELGRLGRRELAELLAGILNEPVPPALVGEILARSEGNPFFAEELLAAHLEGARLPLAL
                     RDLVLARVETLSEPTQRVLEAAAVAGARVDHELLAAVVGQDAEELVRLLREAVSHHVL
                     AVDQSSGAYVFRHALVQEAIYDDLLPVQRAPLHAAYARALDRRIEQRAERQGERQGGA
                     AAAAERGQLAYHWYAAHDQGQALLACVQAGQAAEAASALAEALEHYERALELWDQVPA
                     AAARSSLDRVTVLQRAGEVANLAGRYDRAVALARLAFDRVDEVAEPLRAGALLERLAR
                     YHWLAGDSPTAMLIIERAVAVIPAEPPTRVLARALAAHGQLLMLMSRHSPAQARCEEA
                     VVVARKVGARAVEGHALTTLGTSLSLLGKVEAGIANLEEGRQIAVELGAVDDQCRAHS
                     NLATVLELNGRAADAVGVYLAGVEVGRQLGALGHYGPAQLPDAAAALLSLGRREEAER
                     VLAEAFDLDLVSAAHWVRPLTARGKLRLWEGDLAAARADFQRILDESTAPLDPQVAAP
                     VLAGLAEVAVWDGRLPDARAAVADGLATLASAEEPYWITQLCRVGMAVEAAAAEQARD
                     RHADAEHTAAHQRAAGLIEQMRSATQAPNVVVTPAVAANLLAAEAEWSRVTGPSDPDG
                     WAASAAAWDALSYPWPAAYAHWRRAEALLAQRAPRDDAATALATAWTLASRFDARLLI
                     AELGALARRARIQLPLQPGGGAADGSASEPAPEPDPLGLTPREREVLALIADGRTNRQ
                     IATALFISDKTAGVHVSNILAKLGVASRGEAAAVAHRLRLTG"
     gene            complement(13752..13991)
                     /locus_tag="VF995_09160"
     CDS             complement(13752..13991)
                     /locus_tag="VF995_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343768.1"
                     /translation="MYPMLTRKLAALRIDDLQREAARQRQIRALAARREPATRPRRIP
                     FDFDRLLSRRPRRTLEPRPDPAWPRCAPDGARPTR"
     gene            complement(14156..15256)
                     /locus_tag="VF995_09165"
     CDS             complement(14156..15256)
                     /locus_tag="VF995_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009678829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9343769.1"
                     /translation="MSALDAAEPQAGPNRTGSPSAPLRAALEQLRLDGAIFFRSEFSE
                     AFALEDTPADIAGLLHPGAERLILFHIVARGACWVAGPEGDRHWACQGDVIVVPYGDQ
                     HTIGGEQQAERVPINSLLEPPPWAVIPLLRHGGGGARTDIVCGYLHSEDPLFDPAMRV
                     FPSVFVVRLPAGPAAGWVQASIAYALEESAPSNASANPIATRLPELVLTEVLKLHLAT
                     APAAGQGQGWIAALRDPVLAPALSLLHSAPSRRWTVAELAAGAAVSRSLLDERFRQVL
                     GRSPIRYLTEWRMHLAKELLATTRIGIGTVARRVGYDSEEAFSRAFKRAHGDPPGHWR
                     AARWAAVMAPGGAPRPSTHRMPPGRPVPRSRR"
     gene            15471..15869
                     /locus_tag="VF995_09170"
     CDS             15471..15869
                     /locus_tag="VF995_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9343770.1"
                     /translation="MAAFPPLTHVALTVHDLSVSVPWYEALFDAKPVIDEDTDPDFHH
                     TVYLLGNNTLVGLHQHAKAAPEEAFSEFRVGLDHVSFGCADRAELEQWARRLDDLGVA
                     HGAIKDASYGSGLSFRDPDGIALEFFAPPS"
     gene            complement(16031..>16466)
                     /locus_tag="VF995_09175"
     CDS             complement(16031..>16466)
                     /locus_tag="VF995_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9343771.1"
                     /translation="YFGELLMDAAVRRSDIRCISIRPSWVQHEGNYERNLGPQVRDAV
                     LPSAGLWSYIDVYDLADAIVLAVQSELPGHEVIYVASPDNPGGHDFQRILRQHYGDQI
                     ELRPLARPDASGISSAKAMRLLGWKPTRSWRDYLDENGRAIT"
BASE COUNT         2396 a         5898 c         5989 g         2183 t
ORIGIN      
        1 tcaccgccac gtcgagcacg gcgagcgcgc cgctgtcgag ggcgccacgc cgccggccca
       61 cctccaccac cacggcgctc tcgaccaggc aggcgaccag caccagggcc accaccaccg
      121 gagcccggaa gtgccgccac accaagatcc cggcggtgac ggtctgtgtg tagatcgcca
      181 gccgcacgaa cgcgaacgtc cccagcagca atcgttcgct gcgctgggcg gcgtcaccgg
      241 gcttgcctgc cgagctggcc attttgcacc cttgtgcggc agtctacgag actggggccc
      301 tcaggggaac gcgtcgacga ccgaccacag ggtcagtcgg atggatcgca ccggtccgcc
      361 agcagggccg gcggctgcgg cggcgacgcg gcgctgacct tcccgagctc gatcgccttg
      421 cgggtgaggt cggccttgtt gccgacgcgc agcttctgcc ggatgcgctt gatgtaggtc
      481 tcgacggtcg tctggctgac gcccatccgc ctcgccgcct gcgcgtgggt gaagccccgg
      541 gcgatgtagc gcagcgtctc ctcctcgcgc ggggcgagcc gctgctcgcc gccgccccag
      601 gcgcccacgc ccgcctcgag ggcggagtcg aggtccaggt cggcgtggag catgtcggcg
      661 agctgggacg acaggtacag ctgaccggcg gcgacccgct cgacgccggc caggaaggcg
      721 tcgtcggagg cctgcttggt caggtagccg tcggcgccgg accggatcgc cgccaggacg
      781 tcctcacgcc gccccgacgc cgacatcacc aggacgcggc agcgcgccgc cagccgctcg
      841 atcgccgcca gcgagggcgc ctcccccgcc aggtagagat cgacgatcac cacctccggc
      901 gccgcctcgc ccgcgcccgt ggcgacctcc aactcctcga ccgaggcgac cgagcacagc
      961 acgcggatct cggggtgtgc ctcgaagatg tgctcgacac cccagcgcgc caccggatga
     1021 tcgtcgacga tcgcgatccg gatcggtcgc atccccagcc ctcgagggcg aacggccatg
     1081 acaaccgtat tgtttcacgc cgcggctgcc ggccggcatg tccctggttc aggggacatg
     1141 ccgccgcggc cttcctgctg gacgaaccct ccggcgaact ggtcttcgag gcggtcgcgg
     1201 gcgagggcgc caaccacctc atcggccagc gcgtcccgcg ttccgcgtcg ggtccgggac
     1261 cgggttcggg atggggtcgc gcaaggccgc ggggacctgc gcgcgggcgc ggctggtggc
     1321 ggcgggagac gcgccggcgg cctcaagggc caacagggcg gcaccggtga cgggcggcag
     1381 ggccagggtg atcggctggg cgtggggggc gaccgcctgg atgccgcgat ggacacgggc
     1441 ggtgaggcgg tcgtcgccgg cttggaggat gccgccgccc agaacgatct cggcgtcgga
     1501 gcgggtcagg cgcaggcggc gcagcagggc ggcggccatc gcgacaacct cgtcggcgag
     1561 ccggtcgacg atgccgccgg cgaccccgtc gccgtcgcgg gcggcggcga acagcagcgg
     1621 cgggaggcct tccagcgcgc gttccgggag ccggccctgg aagatggcga tgccggccgc
     1681 ctgtggctgg cgcaggccga aatgggcggc gaccgaccgc tgcagcgagg tgcgcggccc
     1741 gcgcccgtcc tggctgcgga tggcggcggc gagggcggcc atgccgacgt cggcggcgcc
     1801 gccccagtcg ccggtcagcg gtcccagcgc cggatagcgg gcatgccgcc cgctcggcga
     1861 gacggcaacg cagttgatac cggtgccgca caccaccgcg acgccccagc cgcgcgaggc
     1921 gccggcgcgc agcgccgcaa agcagtcatt gccgacgacg ctggcggcgc tccagcggca
     1981 tgccgccacg cgcgcgccga ggtcgcgctc ttgagccggg aaatccagcc cggagaggta
     2041 gtaggcgccc agctcggcga tcgggcgcgg ctgcccgtcc agcccagcca cgagggccgc
     2101 ccgggtgacc agcgcgtcaa ggatcgccac gcagcgggcg gcgccgacgt tgtcggggtt
     2161 ggagccgggg ccgcgcaccg cggccaggac ggcgccgtcc tcgtcgagca gcaccaggtc
     2221 ggtcttggag ccgcccccgt ctgccgcgag gagccgtcgc tggcccatgc tcaggcttcc
     2281 gtctccgtct cagtccgggt ctgggcgccg gcggcggtgc cggccgcggc ggtgccggcc
     2341 gcggccctgg cgccggtggc cgggtccggg aagaagcgcg gcaggtgggc gcggttggcc
     2401 tccagcagtg cctccagcag cgggacggcg acctggtact gggcgaccag cgggttggcg
     2461 agcagggcgc gcagggcgac gcggcggtcg ccgctccgcg ccgcctcgac cgccaggcgc
     2521 tcgtaggcgg cgacctgctg caccagcccg agcagctcgg gggcgagcgg ccgctgcggc
     2581 agcgggtggg cgccgtcgcg gtcgatccgg gcggggacct cgacgacggc ctccggcggc
     2641 agcccggcga gggtggtccc gttgcggagg ttgacgacct ggacgtcgcc gcggccaccg
     2701 acgagcgact cgaccagctg cgcggcagcc tcgctgtaga aggcgccgcc acgctgctcc
     2761 agcagcgccg gtttggcggc gagggcgggg tcgcggtagg cctccagcag ctcctgctca
     2821 atcgccgcta cccgttcggc gcgcgacggt gcggcgcgct gctcggcgag cacctgctcg
     2881 cgcaggtaga agaagcgcag gtagtacgac ggcaccgctc cgagcgtgcg cagcagcgcg
     2941 accggctgtc cggcctgccc ggcgagcacc tgggcggcgt cggggcgcgc cagcagctcg
     3001 gggaggcggt cgacgccgtc caggtgggcg gctctgaccc aggagagatg attgaggccg
     3061 acgtgctcca gctggattcg ctccggctcg acctggcaca gcgccgcgat cgtgcgctgg
     3121 aagccgatag cgacgttgca caggccgatg actcggtggc cggcgtcgag cagggctctg
     3181 gtgacgatcc cgaccgggtt ggtgaagtcc acgatccagg cgcccggcgc cgccagcgcg
     3241 gcgatccggt cggccagctc cagcatgacc gggacggtgc gcagcgcctt ggcgaagccg
     3301 ccgggaccgg tggtctcctg cccgatgcag ccgaaccgca gcggcagggt ctcgtcgacc
     3361 tgccgggcgg cctgcccgcc gacgcggagc tggatgatcg cgacgtcggc cccggcggcg
     3421 gcgccggcca ggtcggtggt cagggtgagc cgccccggcc agccctgccg cgcaaggatc
     3481 cggcgggcga gcccgccgac gacctccagg cgaccggcgt cgagatcgtg gagggccagc
     3541 tcgtcgacgg cgaggcgtcc ctggcgctgg cgtcgggcga acccgtcgac cagctccggg
     3601 gtgtaggtgc tccccccgcc gaccacggcg accttcacgc gcgggcctgc ctcgggcagc
     3661 tcaacccttc accccggtga gggtcacgcc ctcgatgaag gcgcgctggg cgaagaagaa
     3721 cagcgcgatc accggcagca tcaccagcac aaccgcggcc atggtgaggt tccactcgac
     3781 gcggtgcagg gtgcggaact gcgtcagggc gaccgacagc gtccagttcg cctggttgtt
     3841 gatcacatac agcagcggcc cgaagaagtc gttccaggcg tagaggaagt tgaacagcgc
     3901 caccgcggcg agcgccggcc tggccatggg gacgaccacg cgccgcagga tctggaagtc
     3961 gctgccgccg tcgacccggg cggcgtccag gtagtcctcg gggatggtca tgaaaaactg
     4021 gcgcagcagg aagatcgaga aggcgtcgcc gaagaagttc ggcacgatca gcggcagcag
     4081 cgtgccgatc cagtgcaccc tcgtgaagat cacgtacagc gacaccgccg tcacctggta
     4141 gggcagcatc atcgtggcca gcaccagcac gaagaagaac ccgcgcaggc gccagcggag
     4201 cttggcgagc gcgtaggcga ccgggacgct ggacagcagc accccgatgg tggccaggcc
     4261 ggcgtacagg aacgtgttgg cggcgtagcg cagcaccggg aacgagtgga tcacggtggc
     4321 gaagttcccc cagcggaacg ggtgcggcca gaggttctgg ccacccgcct gcaggtcgtt
     4381 catcaacgcg gtcagcacga tgaacgcgaa cggcgccagg aacaggaccg agcatgcgat
     4441 cagcccgctg tgggtggcca ccgcgaccag gaagcggtgc cgccggaccg ccggcggcac
     4501 cgccggccgc gggcggtcaa gggccgggac tgccgcgctc atctgacgcc tccctggtag
     4561 tggacccagc gtctcgagat gcgcagcagc agcagggtga agatcagggt gaccaggaac
     4621 agcgtccagg ccagcgccga ggcgtagccc atgcggaagt actcgaagcc gttctggtag
     4681 agccagaccg agaagaacag ggtcgagccc tgcgggtagc cgagcgtgat gctctggccg
     4741 acctggaagg agccgctggc cgccaccgag gcgacgtagc cctgggtgaa atactgcagg
     4801 ccgtcgatga tgccgatgat cagggcgaac aggatcaccg gcgagatcat gggcagggtc
     4861 accttgcggg ccttctgcca ggcgttggca ccctcgatgt cggcggcctc gtagagctgg
     4921 accggcacgt cgagcaggct cgccaggaag atgatcatga tgtcgccgat gccccacagc
     4981 gccagcagca ccagcgccgg cttggcgaaa cgggggtcgg agaaccacag cggctgaggc
     5041 aggtggagca ggtggaggat gtggttgacc ggcccggtcg tcgggttgaa caggaacacg
     5101 aaaccgagcg tcgccgccac cggcggggcc agcaccggca ggtagaagat cgtccggaac
     5161 agcccggcgg cgcgcctggc cttggtcagc agcatggccg cgccaaccgc gaacaccacc
     5221 cgcgccggca ccgcgaccag gatcacccag acggtgttgc gcaccgccag ccagaaattc
     5281 tggtccttgg tgaacatgta gcggtagttg gccagaccga tgaacctcgg ctgcccgagc
     5341 aggtcgtagt gggtgaacga gaaccatagg ctggcggcca tcgggtagac gaagaacatc
     5401 gagaacccga gcagccacgg ggacatgaac agcagcacgg tcagctttcg gcgcgcccgc
     5461 aggcgccgcg cggggccggc cgcgcggggc cggggcggtg gcttggaccg gatcgccgga
     5521 gccgcccgcc cctcctggac ggccccggtg ccggtccggg cgctggctat ggcgcaccta
     5581 cctgctgtgc cagctggttg tcgttggtct tgtcgacccc ctggagggcc gcctgcaggt
     5641 cgcggacgtt gccggcctgg tacttgacga gcacgtcctg gaagttgttc tgcagcgcgg
     5701 caccgacggg ggtgggcggc acggcggccg acttcgggtt gccgaagatg tcgaggaagg
     5761 tcttgaaatg ctggtccggc ttgatatcgg gcgagctgag cgccgccgtg acggtgggga
     5821 cgttgcgcag gtcgttggag aggccgacct gggcggcggt gtcggtggcc aggaacttca
     5881 ccagcagcca ggcctgctcg ggatgcttgg cgccgcgggg gatgccgatg atgttgccgc
     5941 tgacgaagcc ggcgccgtac tggccgggct ggtcgtcggc ggccgggaac ggcgcggtcg
     6001 cgtagttgag gttcggcttc tcgtcggcca ggaaggcgac ccgccactcg ccgtcgaggt
     6061 tcatggcgag cttgccgctc tcgaacatgt tctgggcgga gaactcgtcg ggcggctgcc
     6121 cggcgaactt gacgaggttc ttgtagccgt accagtcgac gagttgcttc tgccacttgg
     6181 ccatctccgc ccagcgcggg tccgtggaga tgttggattt gccgtcggcg tcgagccact
     6241 gagcgcccca ggactgcccg aggtgcgcga cgctcgcctc atagaagccc cacagcggca
     6301 cgaagccggc caccttgatc gacccgtcgg ggttgcgcac ggtcagcttc tcggcgtcgg
     6361 cgaccagctc gctcatggtc cgcggcggct tggccgggtc caggccggcc ttggtgaaca
     6421 tgtcggtgtt gtagtagagg ccgtaggtgt cggccagcat cggcagggtg cactgcttgc
     6481 ccttgtagct ggtgtagtcg cgggccgcct gcgggatgac gctcaagtcg gtgccgtccg
     6541 ccttggcgta ggacccaaga tcctggaagg cgttggcggc gcagaactcg ccgacgttgt
     6601 cggtggtgaa ggacatggcg gcgtcaggtg ggttgccgcc gtggatcgcg ttgacgatgt
     6661 tctcgtcggt gatggcgtcg gtgagcttca cggtgatcca cggatatctg gcgtggaact
     6721 tggcgaccgc cgcccggatc gccgccttct cgtggttggc gctgaacccc tcccagaagg
     6781 tgatcgtctt cggcgtggtg tcgaccgtct tggaggtcgt gggcgtcttt gcccccccgc
     6841 ccccggcggt gcaggcggca agcgcgaggg cgaccaccgc caggaccgca atccatgctc
     6901 ggcctccgca cgccccgccg accttgttcg cccccgtggc gcgctcaccg tgatgtctca
     6961 tgctgacgcc tccttggcgc tcgcggaccg ggccggcgac ggccgcccgg tgagtgctgg
     7021 tgcttgctcc gtcctggcga agacccgctc ctgcgccgcg agcagggcgg tcgccagcgc
     7081 gccctgcagc acggcgtcgg cgccgagggc cgataccgcc acccgcggcc ggaacgggcc
     7141 acagccgcgc agctcccggg tgaccggttc gagcagcaga tcgccgctgt tgtggccgat
     7201 gccgccgccg aggatgacca gatccgggtc gagcacggcg gcgacggcgg cgatgccgag
     7261 cgccaggtga tgggcctgca ggttcaccgc cgccagcgcg tccgggtggc cgcgccgcgc
     7321 tgccgcgaag acctcctcgg cgttgcgcgc cttggtcatg ccgcactggc gcgccagcgc
     7381 gaccacgccg cgcgccccag ccgcggcctc gaacgcgccg ccgcgtcgcc ccctggccct
     7441 ggcgccgccg gtcccgggtg gcaggggcag cggcaggtag ccaacctcgc cggccgcccc
     7501 ggccgagccg cggtggagct ggccgcccag caccaccccc ataccgatgc cggtgccgat
     7561 cgacagcagc acgaagtcgt ccacctcacg gccgaggccg cgccagcgct cggcgatcgc
     7621 ggccaggttc acgtcgttct cgaactccac ctcgaggccg agctcggcgc ggacctcgtc
     7681 gaccacgccg ggctgctgcc agccgggcag gttgggggcc aggatgaccc gctcgccgcc
     7741 gggcccgagc acgccggggc tgcccagcgt cgcccaggtc accgcggcca gctcgatccc
     7801 ggcgtcggcg gcgacggcgc gcgccagcga gccgatctgc gtgatgagcg tgtggccgct
     7861 gcggatgtgg gcgtgctcgt cgcggcgtgc gaccaccctg ccggtgatat cggtggccgc
     7921 gcagcggacc cagcgccgcc cgacgtcgat gccgagcacc cagccggcgc tggggtccag
     7981 atcgtagagc accgcgctgg cgccccggcc gccgctggtc ctgccgacct cgcggaccag
     8041 gccggcggct tccaggcgcg cgagcgccag cgagacggtg ggcttggaca ggccgctgcg
     8101 ccgggcgatg tcggcccgcg acagcgggcc gaagcggcgg atcaactcga cggcgccgcg
     8161 ctcgttgatc gcccgcagca ggctcggcgt ccccgccgcc atcggcaccg gcgccgggag
     8221 gcgctcggcg gctgcctcga ccggctgatc ggagggttcc cacaccggca tacggatcac
     8281 tccggtctcg tgcgggactg cagattagtt aggatacttc attaactagc cggactttac
     8341 ggcttcgtcc ggaaggtgtc aacgggcgac tcgggctgac ccagccaccc cacggcgcgg
     8401 agccgccgcg ccgggcggag gtgaggccgc caagtcgccg cacctgaccg cggagcgaag
     8461 tcttcgcaga aattaattaa aaggaatatc gcgcagaatt gcatcataca acaagcgtac
     8521 cgatttggcg cacctatggg gttgctccgg gagtgaccgg ctcgcttagt atttgcctcc
     8581 attgcggcac acaaggcgcc gtgaaattcg agggggagag ccgatgaaaa tccgctacgc
     8641 ggccgccatg gcgtccgttg cggtcgccgc gctggcagcg gctgctggaa tgggcccgag
     8701 cgcgaaagcg agcccgacgg ccgccggcgc gaccggagga gcggtcccga tcacggccgg
     8761 gggccgcgcg cacatgttcc cgcgcccgca ggtgctggcc gacgtgcaca acggcgacag
     8821 caggggcggc agcccgccgc tgcagtacca cagcgggcca atcatgaaga cggccgtcaa
     8881 ctacatcgtc ctgtggaatc ccgggcacct gcagaatggc accgcgacgg ggtattcgtc
     8941 gaaatacacc tcgctgctct cccgctggtt caaggacgtt ggcggcggcg gcctctacgc
     9001 caacaacacg cagtactccc agggcggcgg agtgttcgtc cagaacagcg cgaagttcgg
     9061 cggggtcttc accgacacca cgccattccc gaccggccag tgcagcaacc cggacaccgg
     9121 cacgaattgc atcaccgacg cgaatctcgc cgccgaagcc gccgcggtcg cgaaggcaca
     9181 ttccatttcg gccggcctca ccaaggactt cttcgtcttc accccggccg gtgaggggtc
     9241 ctgcttcgac gcgggctgct cgtacccgag ctacacctac tactgcgcct accacagcta
     9301 caccaacgcg accttcggca acagcccata tctgctctac gccaacatgc cctacccgac
     9361 cgacccggtg ggcaacaact gctacgcctc ctacctcggg cagaccttcc cgaacggcga
     9421 cgtcaacgcc gacgcggcga tcaacgtcac ctcgcacgag cagatggaga ccgtcaccga
     9481 cccgctgctg agcgcctggt acgacgcggc cggctatgag atcggcgacg agtgcgcctg
     9541 gaacttcggc agcaacaact ggccgtccgg cggcaaccag gagcgcaacg gccaccccta
     9601 cgacctccag ctggaggcat ccaaccacag cgccgactgc gtgacctcgg gtccctgacc
     9661 cggtccccag tcatcgaaca caggaaggcc ctggcccccc cggggccttc ctgtgttgct
     9721 tccggttgcc gccacccgcc gtgccgctta gcatgaccac caccggatcg ggcaaatcag
     9781 acccgacggg taatcgggaa cgtcaggggt gcacaccggg tgcgcggcga gcacagcgag
     9841 ccggagccga cgctggacat tgacaccagc gtggcgcatc cggcccggat gtacgactac
     9901 ttcctcggcg gcaaggacaa cttcccagct gaccgcgagg ccgccgagaa ggtcctcgcg
     9961 atcgtgccgg aagggcgcga catggcccgg gagaaccggg cgttcctcgg gcgcgcggtc
    10021 cgcttcctcg ccgggagcgg gatccgccag ttcctcgaca tcggcaccgg catccccacg
    10081 cagggcaaca ccaacgcggt cgcgcacgcg gtcgcgcacg acgcccgcgt tgcctacgtg
    10141 gacaacgacc cgatcgtgct ggtccacgcc cgcgcgctgc tggccgccca cgaccaggac
    10201 aggaccacgg tcgttcaggc cgacctgcgg cagcccgcgg agctcctcgc gcatccggag
    10261 gtgcgcgcga tgatcgactt ccagcagccg gtggcgatcc tgctggtggc gatcctgcac
    10321 ttcatccgtg accaggagga tcccgccggg attgtggcct gcctcaagca ggtcatggcg
    10381 ccgggcagct acctggtcat ctcccacgga acccaggacc tcgacccggt gatggcacag
    10441 cgggccgtgc gtccctacaa ccaggccaca gccccgttca tcctgcgctc cagggacgag
    10501 atcgccagct tcttcgacgg cttggagctg gtcgaccccg gcctggtgca gctgcccctg
    10561 tggcggccgg acggccccgt caccgccaac ctggaccgca tctggctcta cggcggcatc
    10621 ggccagcagc catagcgcga ggcgaccgcg gagcgactgc cgggccgcgg ttgctggcgc
    10681 gcgccgttag ccggtgagcc gcagccggtg cgcgacggca gcagcctcgc cacggctcgc
    10741 gacgccgagt ttggccagga tgttggagac gtggacgccg gcggtcttgt cgctgatgaa
    10801 cagcgcggtg gcgatctgac ggttggtgcg gccgtcggcg atcagcgcga gcacctcacg
    10861 ctcccgcggg gtgaggccga gcggatcggg ctccggggcg ggttccgatg ccgagccgtc
    10921 cgccgctccc ccgcccggtt ggaggggcag ctggatccgg gcgcggcggg ccagcgcgcc
    10981 gagctccgcg atcagcagcc gcgcgtcgaa cctgctggcc agggtccacg ccgtggcgag
    11041 ggccgtggcg gcgtcgtcgc ggggggcccg ctgggccagc agtgcctcgg cccgtcgcca
    11101 gtgggcgtag gccgccggcc aggggtagct gagcgcatcc cacgccgcgg cgctggccgc
    11161 ccagccgtcg gggtcgctcg ggccggtgac ccgggaccac tcggcctcgg cggcgagcag
    11221 gttggccgcc accgccgggg tcaccaccac gttgggcgcc tgcgtcgcgc tgcgcatctg
    11281 ctcgatcagg ccagccgccc gctggtgggc ggccgtatgc tcggcgtcgg cgtggcggtc
    11341 gcgggcttgc tcggccgctg ccgcctcgac ggccatcccg acgcggcaca gctgggtgat
    11401 ccagtagggc tcctcggcac tcgccagggt cgccagcccg tcggcgacgg cagcgcgtgc
    11461 gtccggcagg cggccgtccc agacggcgac ttcggccagg ccggcgagca ccggggcggc
    11521 gacctgcgga tccagcggcg cagtggactc gtcgaggatc cgttggaagt cggcccgcgc
    11581 ggcggcgagg tcgccttccc acagccgcag ctttccacgg gcggtcaacg ggcggaccca
    11641 gtgcgcggct gacacgaggt cgaggtcgaa cgcctcggcc agcacccgct cggcctcctc
    11701 ccggcgcccc aggctgagca gcgcggcggc ggcgtcgggg agctgcgcgg ggccatagtg
    11761 gcccagcgct ccgagctggc gcccgacctc gacgccggcg aggtagacgc cgacggcatc
    11821 ggcggcgcgg ccgttcagct ccaggacggt cgcgaggttg gagtgcgccc ggcactggtc
    11881 gtcgacggcg ccgagctcca cggcgatctg gcggccctcc tccagattgg cgatgccggc
    11941 ctcgaccttt ccgaggaggc tcagcgaggt gccaagcgtc gtgagagcgt ggccctccac
    12001 ggcgcgcgcg ccgaccttgc gggccaccac caccgcctcc tcgcagcggg cctgcgccgg
    12061 cgaatggcgc gacatcagca tcaggagctg gccgtgcgcg gccagcgcgc gcgccagcac
    12121 ccgcgtcggc ggctcggctg ggatcaccgc gactgcccgc tcgatgatca gcatcgccgt
    12181 ggggctgtcc ccggcgagcc agtggtagcg ggcgagccgc tcgagcagcg cgccggcccg
    12241 tagcggctcg gcgacctcgt cgacccgatc gaacgccagg cgtgccaggg cgaccgcgcg
    12301 gtcgtagcgg ccggcgaggt tggccacctc gccagcgcgc tgcaggaccg tgacccggtc
    12361 cagcgaactg cgggcggccg ccgcgggcac ctggtcccac agctccagcg cgcgctcgta
    12421 gtgctcgagc gcctcggcca gcgccgaggc cgcctccgcg gcctgcccgg cctggacgca
    12481 ggccagcagc gcctgtccct ggtcgtgggc ggcgtaccag tgataggcga gctggccgcg
    12541 ttcggcggcg gcggcggcac cgccctgccg ctcgccctgc cgctcggcgc gctgctcgat
    12601 gcgacggtcc agcgcccggg cgtaggcggc gtgcaggggg gcgcgctgca ccggcagcag
    12661 gtcgtcgtag atcgcctcct gcaccagtgc gtggcggaag acataggcgc cgctcgactg
    12721 atcgaccgcc agcacgtggt ggctgaccgc ctcgcgcagc aaccgcacca gctcctcggc
    12781 atcctggccg accactgccg ccagcagttc gtggtccacc cgcgcgccgg ccacggccgc
    12841 agcctccagc acccgttggg tcggctccga gagcgtctcc acccgcgcca gcaccaggtc
    12901 ccgcagcgcc agcggcagcc gagcgccttc caggtgggcg gcgagcagct cctcggcgaa
    12961 gaacgggttg ccctccgagc gcgccaggat ctccccgacc agcgccggtg ggaccggctc
    13021 gttgaggatc ccggcgagca gctcggccag ctcgcggcgg cccagccggc caagctccag
    13081 ccgctcggcc cggccgctgc ggtccagctc ggccaggaac ggccgcagcg gatggcgccg
    13141 gtgcagctcg tcggagcgat aggtcaccac cagtgccacg ccgccgcgca agttgcgggc
    13201 caggaagccc agcaggttgc gggtggactg atccgcccag tgcaggtcct cgaccaccag
    13261 cagcaccggg gcccgctcgg cgaggcggtg cagcatcccg agcagcagct cgaacagacg
    13321 cgtcggcacc agtggccccg gcgccggtgc gacctccccg ccgtcctgcg gggccagttc
    13381 cgggaccagc cgggtcagct ccccacgggc ggcgccgagc gcccgctcca gctccgcggg
    13441 gtcgatgccg cgggcgagcg agcgcagcgc ttccaccagc ggcgcgtagg ccaggacgcc
    13501 ctcgccgacg tccaggcagc caccggtgag cgcgacggca ccgacgccgg cggcgtcgtg
    13561 gagcagctcg gcgaccagcc gggtcttgcc gacgccagcc tcgcccgcca ccaccacgat
    13621 ggcgggctgg ccggccgctg ccccgtcgag cgcctgcctg agccgcgcga cctcggcgtc
    13681 gcggccgacc agttccagac aggagactcg ccgggccacg ccctcgatca tctcagatcc
    13741 cgcacggtgg ctcaccgggt cggccttgcg ccgtcgggcg cacaccgtgg ccacgccggg
    13801 tcgggacgcg gctccagcgt gcggcgtggg cgccgcgaca gcagccggtc gaagtcgaag
    13861 gggatacggc gggggcgggt ggccggttct cgcctggccg ccagcgcgcg gatctggcgc
    13921 tgcctggcgg cctcccgctg gaggtcgtcg atgcgcaggg cagcgagttt ccgggtcagc
    13981 atcgggtaca tctcggttcg ttctcctcag gtgccgtgtg cgaggtggcg tggacgtcct
    14041 gctcgaagcc gagcatccgc ctaagacccc tcctggcaca ttgggaggac gaggtactgc
    14101 cgtacgggtc agcgcctcat tttcgcccgc gtccggctca gcgcggcggc ggcgatcacc
    14161 ggcgtgagcg tggtaccggt cgccccggcg gcattcggtg cgtgctcggc cgcggggcgc
    14221 ctcccggcgc catgaccgct gcccagcgtg cggcgcgcca gtgccccgga gggtcgccgt
    14281 gcgcccgctt gaacgcgcgg ctgaaagcct cctccgagtc gtacccgacg cgcctggcaa
    14341 ccgtgccgat gccgatcctg gtggtggcca gcagctcctt cgccaggtgc atgcgccact
    14401 cggtgaggta gcggatcggt gagcggccca ggacctggcg gaagcgctcg tcaagcagcg
    14461 agcgcgagac ggccgcgccg gccgcgagct cggcgaccgt ccagcggcgg ctgggcgcgc
    14521 tgtgcagcag cgacagcgcc ggcgccagca ctgggtcgcg cagcgcggcg atccatccct
    14581 ggccctggcc ggctgcgggc gcggtcgcga ggtgcagctt gaggacctcg gtcagcacca
    14641 gctcgggcag gcgggtggcg atcgggttgg cgctggcgtt ggagggcgcc gactcctcga
    14701 gcgcgtaggc gatgctggcc tggacccagc cggccgcggg tcccgccggc aggcgcacga
    14761 cgaacacaga ggggaacacg cgcatcgccg ggtcgaacag cgggtcctca gaatgcagat
    14821 agccgcagac gatgtcggtg cgtgcgccgc ccccgccgtg gcggagcagc gggatgaccg
    14881 cccacggcgg cggctcgagc agcgagttga tcggcacccg ttcggcctgc tgctcgccgc
    14941 cgatggtgtg ctgatcgccg tagggcacca cgatgacgtc cccttggcag gcccagtggc
    15001 ggtccccttc ggggccggcg acccagcacg cgccgcgcgc gacgatgtgg aacaggatca
    15061 accgctccgc gcccgggtgg agcaggccgg cgatgtcggc cggggtgtcc tccagcgcga
    15121 aggcttcgct gaactcggag cggaagaaga tcgcgccgtc gaggcggagc tgctccagcg
    15181 ctgcccgcag cggcgccgac ggcgacccgg tgcggtttgg cccagcctgc ggctcagccg
    15241 cgtccagcgc gctcatttgg cacgctcctc catcgtcgtc caccacgggc accgcgggca
    15301 cgcgcgcagc gcggtgtccc accgtcgcaa ctccggtgtc gcggtcagca gtggcggtgt
    15361 ctcgatcata gcgacgcaat cagctccgat gaacactcaa tcatgacagc agtccccgca
    15421 ccggatcggt tcccatcagt tcccatcagt tcccgagaag gagcaacccg ttggccgcct
    15481 ttccaccgct cacccacgtc gccctcaccg tccatgatct gtccgtcagc gtgccctggt
    15541 acgaagcgct cttcgacgcc aagccggtga tcgacgagga caccgatccc gacttccacc
    15601 acaccgtcta cctgctcggc aacaacacgc tggtcggcct gcaccagcac gcgaaggccg
    15661 cccccgagga ggccttcagc gagttccggg tcggcctcga ccatgtctcc ttcggctgcg
    15721 ccgaccgcgc ggagctggag cagtgggccc gccggctcga cgacctcggc gtcgcgcacg
    15781 gcgccatcaa ggatgcgagc tacgggtcgg gcctgagctt ccgtgaccca gacggcatcg
    15841 cgctcgagtt cttcgccccg cccagctgac ccgccgggcg gacagggggg cggccagagc
    15901 tttgaacgct cgggtcaccc cccgaccggc tcgccggcgc aggcggcgac gacctccgtg
    15961 tgccaggcga cgtcggcccc ggtggcgacc gggccgacca gcgccaggtt gcggccgggg
    16021 accctgggcg tcaggtgatc gcgcggccgt tctcgtccag gtagtcgcgc caggaccggg
    16081 tcggcttcca gccaagcagc cgcatcgcct tggccgagga gatccccgag gcgtcggggc
    16141 gggccagcgg ccgcagctcg atctggtcgc cgtagtgctg ccggaggatc cgctggaagt
    16201 cgtggccgcc ggggttgtcc ggcgaggcga catagatcac ctcgtggccg ggcagctcgg
    16261 actgcaccgc cagcacgatg gcatccgcca ggtcgtagac gtcgatatag ctccacaggc
    16321 ccgcgctggg caacacggcg tcgcggacct gcgggccgag gttgcgttcg tagttgccct
    16381 cgtgctggac ccagctcggg cggatcgaga tgcagcggat gtcggagcga cgcaccgcgg
    16441 cgtccatcag cagctcgccg aagtac
//
LOCUS       DASWDC010000284         5065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_457949, whole genome shotgun sequence.
ACCESSION   DASWDC010000284 DASWDC010000000
VERSION     DASWDC010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457949"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..802
                     /locus_tag="VF995_09180"
     CDS             164..802
                     /locus_tag="VF995_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9343772.1"
                     /translation="MAPLLGAVLDPTLFNVSELIREGYDDAHSATLPLIVQHGSGALA
                     APLASGAKGGRQLHALGAVVARQPKTSLAQLGAAMAAPARAARQAQARAAATAPEVAG
                     AKHVWLDRKLHVGALPAATAPATTTVPSATLDHDLTQIGAPAAWNAGFSGKGVSVAIL
                     DTGIDTTHPDLQGKVLDAANFSDASDTTDHFGHGTPRRRHRRRDRRGLQWRP"
     gene            780..2333
                     /locus_tag="VF995_09185"
     CDS             780..2333
                     /locus_tag="VF995_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9343773.1"
                     /translation="MASNGARKGVAFGASLLNGKVLDDDGFGTDDTVIAGMEWAAQHH
                     AKVVNLSLGEDVPSDGSDPASQTVDQLTAADHTLFVIAAGNSGPAPVGSPAAADAALT
                     VGAVDGNDQLANFSTGGPRLGDDAIKPELTAPGVDIIEARAAGTTLGIPVGKDYVTLS
                     GTSMATPHVAGAAAILAQQHPDWSPEQLKAALVATAAPASGGDELQVGSGRVDIASAL
                     GIPVVGGQAAVNLGVFAWPQTSASLVTRTLTWTNPGTSPVTLDLGVQLRSRAGGSTPA
                     GLASLSPATLAVAPGGSASATLTVSPAAASSAPGLYDGAVTATPRGGRGTMHTLLDFD
                     EQPPSHDLTVKAVPLPGTPTGATIGVVDVVNIDDASQFAQFQTATETLYGIPPVSTRS
                     STVWTFHSAAPASDTDLAPVPLLLLDYQLPLDLNNHPDGTQATFQVHPQDGTPAQTIS
                     QFTLETSTDDGATWTQAPSHALGAGRFQATLPAAAPGTWMALRADARSTTGNRVQQTI
                     IHAYRTATP"
     gene            2355..2684
                     /locus_tag="VF995_09190"
     CDS             2355..2684
                     /locus_tag="VF995_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343774.1"
                     /translation="MTQQPVSTRDRLQARGRSLHADPELAKLDSVAALELVGEALAEA
                     RADIYNPLPAMLDDLRLSLSLRQLARAHGAAPAGLPGLRSLLRRTRPLRTYLPGSRVT
                     TETEVTG"
     gene            2681..>5065
                     /locus_tag="VF995_09195"
     CDS             2681..>5065
                     /locus_tag="VF995_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX9343775.1"
                     /translation="MTQELELELNAKLAALTLERKVALLTGADFWSLHPEPAIGLRRV
                     VLSDGPAGVRGERWDERDPSANVPCPSALAATWDEARVERLGRLLAAEARRKGVQVLL
                     APTVNLHRTPYAGRHFECFSEDPLLTARIGAAYVRGVQAGGVAATVKHFVANDAETER
                     HTVDVRVDERTLRELYLAPFEAIVREAGAWAVMAAYNRVNGTTMTEHPLLREVLKAEW
                     GFDGVVMSDWFATTSTVPSARGGLDLVMPGPSGPWGDDLVAAVRAGQVSEAAIDDKVL
                     RVLRLAARVGGLDLGADRVEAEREPWPAAAVAAELRATAAAGFVLLRNEPAAAPAWAA
                     AGAGPRERGSALLPLEAGRLRRVAVLGPNAAVPRTLGGGSATVFPPYAVSPLDGLRAA
                     LEPGVEVTHAAGARTTTRVPAADLRLLRPPASALEGLEGAERGVELRFLTADGTVLHH
                     ERRRTAAFTWLGSFGDFPAERVAAIELRTRLLADVSGEYTVGCSGVGDFELALAGPTR
                     FTGPVTLPPGAEPIDAMMRPPQRVARVPLEAGEEIPVTLRYGLTEASRIPAFQLNVER
                     PHGGDEEELARAVALARNADAAIVVVGTTDEVESEGFDRDSLSLPGRQDDLVRAVAAA
                     NPRTVAVVNAGAPVLLPWAGEVPAILLTWFPGQEFGNALADVLLGATEPGGRLPPTWP
                     ASPDGLPTTTPTAGRLDYAEGSFVGHRAPEGRAVQYPFGHGLGYTTWEYLDLKVTGGA
                     TLDPDPAASVAAQVTVRNSGHRPGRELVQVYASRPDSTIPRPARWLAGFAPADAA"
BASE COUNT          684 a         2117 c         1676 g          588 t
ORIGIN      
        1 gcctactaca accgattccg gctgcactcc acccgcggct acctgccacc aatcgagtgg
       61 gaacgacagc acgccaccat caacccgcta ccatcgacca cggccgcata gcctcagtgt
      121 ccgcctcccg gggggaattc cgctgcacgt cgtcccgctg gacgtggcgc cgctgctcgg
      181 cgcggtgctc gacccgaccc tgttcaacgt cagcgaactg atccgtgagg gctacgacga
      241 cgcccacagc gccaccctcc cactgatcgt ccagcatggc agcggcgcac tggccgcccc
      301 gctggccagc ggcgccaagg gcggccggca gctccacgcc ctcggcgcgg tggtggcgcg
      361 ccagcccaag accagcctgg cccagctcgg cgccgccatg gccgctccgg ctcgcgccgc
      421 ccgccaggcc caggcccgcg ccgccgccac ggcgcccgag gtcgccggcg ccaagcacgt
      481 ctggctcgac cgcaagctcc acgtcggcgc cctcccagcc gccacggccc ccgcgaccac
      541 caccgtcccg tccgcgacac tcgaccacga cctgacccag atcggcgcgc cggccgcctg
      601 gaacgccggc ttcagcggca agggcgtcag cgtcgcgatc ctcgacaccg gcatcgacac
      661 cacccacccc gacctccagg gcaaggtcct cgacgccgcc aacttctccg acgccagcga
      721 caccaccgac cacttcggcc acggcacccc acgtcgccgc caccgtcgcc gggaccggcg
      781 tggcctccaa tggcgcccgt aagggcgtcg cgttcggcgc cagcctgctc aacggcaagg
      841 tgttggacga cgacggcttc ggcaccgacg acaccgtcat cgcggggatg gagtgggccg
      901 cccagcacca cgccaaagtc gtcaacctga gcctcggcga ggacgtgccc agcgacggca
      961 gcgacccggc gtcccagacg gtcgaccagc tgaccgcagc cgaccacacc ctgttcgtga
     1021 tcgccgccgg gaacagcggg ccggcgccgg tcggctcgcc cgcggcggcg gacgccgcgc
     1081 tgaccgtcgg cgcggtcgac ggcaacgacc agctcgccaa cttctccacc ggcgggccgc
     1141 gccttggcga cgacgcgatc aagccggagc tgaccgcgcc gggcgtcgac atcatcgagg
     1201 ccagagcggc cggcaccacc ctcggcatcc cggtcgggaa ggactacgtc acgctgtccg
     1261 gcacgtcgat ggcgaccccg cacgtcgctg gcgccgccgc gatcctcgcg cagcagcatc
     1321 ccgactggag ccccgagcag ctcaaggctg ctctggtcgc aaccgccgcg ccggccagcg
     1381 gcggcgacga gctccaggtc ggctcgggac gcgtcgacat cgcctcggcg ctgggcatcc
     1441 cggtggtcgg cgggcaggcg gcggtcaacc tcggcgtctt cgcctggcca cagaccagcg
     1501 cgagcctggt cacccgcacg ctgacctgga ccaaccctgg gaccagcccg gtcaccctcg
     1561 acctcggcgt gcaactgcgc tcccgtgctg gcgggtcgac gcccgccggg ctggccagcc
     1621 tgtcgccggc cacgctcgcc gtggcgcctg gcgggtcagc ctcggccacc ctcacggtct
     1681 ccccggccgc cgccagcagc gcgcccggcc tctacgacgg agcggtcacc gccaccccgc
     1741 gaggcggccg tggcacgatg cacaccctgc tcgacttcga cgagcagccc ccgagccacg
     1801 acctgaccgt caaggccgtg ccgctgcccg gtacgcccac cggcgcgacg atcggcgtgg
     1861 tggacgtcgt caacatcgac gatgcgtccc agttcgcgca gttccagacc gccaccgaga
     1921 cgctgtacgg cataccgccg gtgtccacca ggtcgtcgac ggtgtggacc ttccactccg
     1981 ccgcgccggc cagcgacacc gacctggccc ccgtgccgct gctgctgctc gactaccagc
     2041 tcccgctcga cctgaacaac catcccgacg gcacccaggc gaccttccag gtccatcccc
     2101 aggacggcac gcccgcccag accatcagcc agttcaccct ggagacctcg accgacgacg
     2161 gcgccacctg gactcaggcg cccagccacg ccctcggcgc tggccgcttc caggcgaccc
     2221 tgccggccgc cgccccaggc acctggatgg cactccgcgc cgacgcgcgg agcaccaccg
     2281 gcaaccgcgt ccagcagacc atcatccacg cctaccggac ggcgacccca tgagcccgcg
     2341 gaaacgatga cggggtgacc cagcagccgg tctcgacccg ggaccggctg caggcccgcg
     2401 ggcgcagcct ccacgccgac ccggagctgg ccaagctcga ctcggtcgcg gcgctggagc
     2461 tggtaggcga ggcgctggcg gaggcacgcg ccgacatcta caacccgctg cccgcgatgc
     2521 tggacgacct ccggctctca ctcagcctgc ggcagctggc gcgggcgcac ggcgccgccc
     2581 cggccggcct gcccggcctg cgctccctcc tgcggcggac gcggcccctc cggacctatc
     2641 ttccaggcag ccgcgtcacg acggagacgg aggtcaccgg atgactcagg agctggagtt
     2701 ggagctgaac gccaaactgg ccgcgctcac cctggagcgg aaggtggcgc tgctcaccgg
     2761 cgcggacttc tggtcgctgc acccggagcc cgcgatcggg ctgcggcggg tggtgctgtc
     2821 ggacggcccg gcgggcgtgc gcggcgagcg ctgggacgag cgcgacccgt ccgcgaacgt
     2881 gccgtgcccc agcgcgctcg ccgcgacctg ggacgaggcc cgggtcgagc ggctcggccg
     2941 cctgctggcc gccgaggccc gccgcaaggg cgtccaggtg ctgctcgccc cgaccgtgaa
     3001 cctgcaccgc accccctacg ccggccgcca cttcgagtgc ttcagcgagg acccgctgct
     3061 gaccgcccgg atcggcgccg cctacgtccg cggggtccag gccggcgggg tcgccgcgac
     3121 cgtcaagcac ttcgtcgcca acgacgccga gactgagcgg cacaccgtgg acgtgcgcgt
     3181 cgacgagcgc accctgcgcg agctgtacct ggcccccttc gaggcgatcg tgcgggaggc
     3241 cggcgcgtgg gcggtgatgg ccgcctacaa ccgcgtcaac ggcaccacca tgaccgagca
     3301 ccccctgctg cgcgaggtcc tcaaagcgga gtggggcttc gacggcgtgg tgatgtccga
     3361 ctggttcgcc accacctcga cggtcccgag cgcccgcggc ggcctcgacc tggtcatgcc
     3421 cggcccgtcc ggcccatggg gcgacgacct ggtcgcggcg gtccgtgccg gccaggtcag
     3481 cgaggcggcg atcgacgaca aggtgctgcg cgtgctgcgc ctggccgccc gcgtcggcgg
     3541 cctcgacctc ggcgccgatc gggtggaggc agagcgggaa ccatggcccg ccgccgcggt
     3601 cgccgccgag cttcgtgcca ccgccgccgc ggggttcgtc ctgctccgca acgagcccgc
     3661 cgccgccccc gcgtgggcgg cggcgggggc ggggccgagg gagcgcggga gcgcgctgct
     3721 gcctctggag gcgggccgcc tgcgccgggt ggcggtgctg gggccgaacg cggcagtccc
     3781 ccgcaccctc ggcggtggca gcgccacggt gttcccgccc tacgccgtct ccccgctcga
     3841 cggcctccgc gccgccctgg aacccggggt cgaggtcacc cacgcggccg gagcgcggac
     3901 caccacccga gtcccggccg ccgacctgcg cctgctgcgc cctccggcgt cagcgctgga
     3961 aggtctggaa ggtgcggagc gcggggtcga gctgcgcttc ctcaccgccg acggcaccgt
     4021 gctccaccac gagcgccgcc gcaccgccgc cttcacctgg ctggggtcgt tcggcgactt
     4081 ccccgccgag cgggtcgccg ccatcgagct gcgcacccgc ctgctcgccg acgtcagcgg
     4141 cgagtacacc gtgggctgct ccggcgtcgg cgacttcgag ctggcgctcg ctgggccgac
     4201 ccggttcacc ggtccggtga ccctcccgcc aggcgcggag ccgatcgacg ccatgatgcg
     4261 gccaccccag cgggtcgccc gggtcccgct ggaggccggc gaggagatcc cggtcaccct
     4321 gcgctacggc ctcaccgaag ccagccgcat ccccgccttc cagctgaatg tggagcgccc
     4381 gcacggcggc gacgaggagg agctggcccg cgccgtggcc ctggcccgta acgccgacgc
     4441 cgccatcgtc gtcgtcggca ccaccgacga ggtcgagagc gaaggcttcg accgcgactc
     4501 cctttccctc cccggccgcc aggacgacct ggtccgcgcc gtcgccgccg ccaacccccg
     4561 caccgtggcc gtggtcaacg ccggcgcccc cgtcctcctt ccctgggccg gcgaggtccc
     4621 ggcgatcctg ctcacctggt tccccggcca ggagttcggc aacgccctcg ccgacgtcct
     4681 cctcggcgcc accgaacccg gcggccgcct cccccccacc tggcccgcct cccccgacgg
     4741 cctcccgacg accactccga ccgccggccg cctcgactac gccgaaggca gcttcgtcgg
     4801 ccaccgcgcc cccgagggcc gagccgtcca gtaccccttc ggccatggcc tcggctacac
     4861 cacctgggag tatctcgacc tgaaggtcac cggcggcgcc accctggacc cggacccggc
     4921 cgccagcgtc gccgcccagg tgaccgtccg caacagcggc caccgccccg gccgcgagct
     4981 cgtccaggtc tacgcctccc gccccgacag cacgatcccg cgcccggccc gctggctcgc
     5041 cgggttcgcc cccgccgacg ccgcc
//
LOCUS       DASWDC010000285         6216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1497854, whole genome shotgun sequence.
ACCESSION   DASWDC010000285 DASWDC010000000
VERSION     DASWDC010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497854"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VF995_09200"
     CDS             <1..949
                     /locus_tag="VF995_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9343776.1"
                     /translation="LPFSSRGLVACLREGAARFGWGDRDPAPGRRRDGRWQVGTGVAA
                     STYPAHPAYRRGAPSTARIRALPDGRFLVQLGASDIGTGAWTALTQIAADALAVPVEA
                     VELQIGDSALPEAPIAGGSRGTGTWGWAIVDAAERLRAKLADGEPGELPAEGVAADGE
                     VPPNPATERFSMHAFGAQFAEVRVDQDTGEIRVPRLLGVFAAGRIVNPKTARSQLLGG
                     MTMGLSMALHEAGVLDQRFGHVANGDLAGYHIATNADVGSVEVVCLDEHDPHVNPMGT
                     KGLGEIGIVGTAAAIANAAWHATGIRVRDLPLSVDKLLA"
     gene            complement(967..2100)
                     /locus_tag="VF995_09205"
     CDS             complement(967..2100)
                     /locus_tag="VF995_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343777.1"
                     /translation="MPMLSRRFLVNAAGAAVVVLVLLLAGRLSGLTDRHGPGGGAAAP
                     APARGDAPATPRLAVIATIPFSESITAISADPTSVWVAACTVTRVDPRNDEPVANVVG
                     TGSSAGTTCVEDVAIGPGVVWGSVPGIGLVRIDPAAAQVAAQVPLPQLLPPIAVSATG
                     VWAVCCQTAPRPLTAGTLIRVDPATNHVAARIRLPGQPVGVAAGPSGVWVVGSDPTGA
                     GRVWRVDPSSGRLVATIDTGSRLGQSVAADGAAVWVDAQPQTGQKGQASRDNPAELLR
                     IDPGSDQVVATYPGATGLGVATIGGTTWTTAPDGLLRLAATTATRAGGGASAGSGTLY
                     PVALDSDAPLAISDLAASQQSLWIATWSGVEGGPGLLRVRLPA"
     gene            2222..2347
                     /locus_tag="VF995_09210"
     CDS             2222..2347
                     /locus_tag="VF995_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343778.1"
                     /translation="MGERPGGKDPDGQGEELIMELTIVNPPPRPEAHQPGTDNRQ"
     gene            2374..3204
                     /locus_tag="VF995_09215"
     CDS             2374..3204
                     /locus_tag="VF995_09215"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9343779.1"
                     /translation="MSTASTTPPNPQFAGKVALVAGASRGIGAVTAQAFARAGAAVVL
                     AARDQRALEQVAESIRNDGGQALVVPTDVGDAASVERLVGQALDTFGRLDAAFNNATG
                     GPLPAQLAEIDPDEFDTGIRVNVRGTFLGMKYEIPALLASGGGTVVNMASVAGIQAVA
                     NLAAYAAGKAGIIALTKVAALDYADQGVRVNVVAPGPILTYHLEAAGPEAQRQAGQST
                     PMRRVGQAEEVAATVLWLCSDQSSFVTGAVVPIDGGQSAGLKPPRMYRQGQSMAAAKP
                     "
     gene            complement(3376..3933)
                     /locus_tag="VF995_09220"
     CDS             complement(3376..3933)
                     /locus_tag="VF995_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009713089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9343780.1"
                     /translation="MARISLDPPGSLSYRIGAWFIRRRFGDDLDPFRAMGHHKQVARA
                     IGQLEQRVPRWHRMDEQLKDLAELAATARIGCPWCMDFGYWVLLGHGITHEKIAAVPR
                     WRDSDLFNPLERLVMEYAEAMTETPPTVDDELVKRLREHFDEAQLVELTMAVCMDNLR
                     SRFNLALGLTPQGFKDRCELPQPSR"
     gene            4018..4920
                     /locus_tag="VF995_09225"
     CDS             4018..4920
                     /locus_tag="VF995_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005320538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9343781.1"
                     /translation="MNQHDWLAERFEADRGHLRAVAYRMLGSLAEADDAVQEAWLRLS
                     RADTSDVQNLSGWLTTVVARVCLNLLRARRARREEPIDVDGQRSVPELAAGRASGADP
                     EQEALLADSVGLALLVVLDTLSPAERLAFVLHDLFAVPFDEIAPILERSPTAARQLAS
                     RARRRVHGHDPEALPDADLHRQREVVDAFLAASRHGDFEALLAVLDPDVVLRADRSAA
                     HTATATEIRGAAAVAKGALAFQGRSRFAQLALVHGAVGVVVAPRGRLFVVLGLTVRRG
                     RITAIDVIADPERLRQLDLAVFDA"
     gene            5044..5286
                     /locus_tag="VF995_09230"
     CDS             5044..5286
                     /locus_tag="VF995_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343782.1"
                     /translation="MTEPFPFSDFSDFSDEDPPIEDASINQHRATDDPHSKVFDDRGG
                     PVEDVTSVSGWPDPGFEDVAFPSPGGGGSGPAGGER"
     gene            5283..6020
                     /locus_tag="VF995_09235"
     CDS             5283..6020
                     /locus_tag="VF995_09235"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEX9343783.1"
                     /translation="MTATSVMTVIDRLRAIELGEAKKSAASEPGTVRNPFGSNNVKYN
                     TAFYGHEVSDPAGKQFAWCVVFQWWCMQAAGVPTSIFPKSAGVSFVRDWFDDPHRRRY
                     FRVPTTPQVGDLVIFKYSHIGFVESVDPTFVVTVEGNSSNRVRRVQHRRDEAEIDGYC
                     RPAYDHVQVQAQGGLSMADVNDILAKLELLRVGERKGTAADPHDFASLEGVGMKVDAA
                     RAEIDWLMAAVKAIAAKVGAQLPPPPQ"
     gene            6122..6216
                     /locus_tag="VF995_09240"
                     /pseudo
     CDS             6122..6216
                     /locus_tag="VF995_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018510728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
BASE COUNT          881 a         2295 c         2191 g          849 t
ORIGIN      
        1 cctgccgttc tccagccgcg ggctggtcgc ctgcctgcgg gagggcgccg cgcgcttcgg
       61 ctggggggac cgggacccgg cacccggccg gcgccgcgac gggcgctggc aggtcggcac
      121 cggcgtggcc gcctcgacct acccggccca ccccgcctac cggcgcggcg ctccctcgac
      181 cgcgcggatc cgcgccctgc cggacggccg cttcctggtg cagctcggcg catccgacat
      241 cggcaccggg gcctggaccg ccctcaccca gatcgcggcg gacgcgctgg cggtgccggt
      301 ggaggcggtg gagctgcaga tcggcgacag cgccctgccg gaggcgccga tcgccggcgg
      361 atcgcgcggg accgggacct ggggctgggc gatcgtcgac gccgccgagc gcctgcgcgc
      421 caagctcgcc gacggcgagc cgggggagct ccccgccgag ggggtagccg ccgacggcga
      481 ggtgccgccg aacccggcga ccgagcgctt ctcgatgcac gccttcggcg cccagttcgc
      541 cgaggtccgc gtcgatcagg acactggcga gatccgggtg ccgcggctgc tcggggtgtt
      601 cgccgccggc cggatcgtca accccaagac cgcccgctcc cagctcctcg gcgggatgac
      661 catgggcctg tcgatggcgc tgcacgaggc gggcgtgctc gaccagcgtt tcggccatgt
      721 ggccaacggc gacctcgccg gctaccacat cgccaccaac gccgacgtcg gcagcgtcga
      781 ggtggtctgc ctcgatgagc acgacccgca cgtcaacccg atgggcacca agggcctcgg
      841 cgagatcggc atcgtcggca ccgccgccgc gatcgccaac gccgcctggc acgccaccgg
      901 gatccgcgtc cgggacctgc cgctgtcggt ggacaagctg ctcgcctgag cccggggcgg
      961 cgccggtcag gcgggcaggc ggacgcgcag caggccgggg ccgccctcca ccccgctcca
     1021 ggtggcgatc cagagcgact gctggctggc cgccaggtcg gagatggcga gcggggcgtc
     1081 cgagtcgagg gcgaccgggt agagggtgcc gctcccggcg ctggcgccgc cgccggcacg
     1141 ggtcgccgtg gtcgcggcga gccgcaggag gccgtcgggg gcggtggtcc aggtggtccc
     1201 gccgatggtg gccacgccga gcccggtcgc gcctgggtag gtggccacca cctgatcgct
     1261 gcctgggtcg atgcgcagca gctccgctgg attgtcccgg ctggcctggc ccttctggcc
     1321 ggtctggggc tgggcgtcga cccagaccgc cgcgccgtcg gccgccaccg actgccccag
     1381 cctgctcccg gtgtcgatgg tggcgaccag ccgcccgctg gacgggtcca cgcgccagac
     1441 gcggccggcg cccgtggggt ccgatccgac cacccagacg ccgctcgggc cggcggcgac
     1501 gccgaccggc tggccgggca ggcggatcct ggccgcgacg tggttggtgg cggggtcgac
     1561 gcggatgagc gtgccggctg tcagtggccg cggcgcggtc tggcagcaca ccgcccagac
     1621 accggtggcg ctcacggcga tcggcggcag caactgcggc agcggcacct gggcggccac
     1681 ctgggccgcc gcggggtcga tgcggaccag cccgatcccc gggaccgagc cccagaccac
     1741 cccggggccg atcgccacgt cctcgacgca ggtcgtgccc gcgctgctgc cggtgccgac
     1801 gacgttcgcc accggctcgt cgttgcgcgg gtccacccgg gtcaccgtgc acgccgccac
     1861 ccagaccgag gtcgggtcgg cgctgatcgc ggtgatgctc tcggagaacg ggatggtggc
     1921 gatgaccgcc aggcgcggtg tggccggggc gtcgccacgg gcgggcgctg gcgcggcggc
     1981 gccgcctccc ggcccatgcc tgtccgtcag gccggaaagc cgcccggcga gcagcaacac
     2041 gagcacgacc acagccgccc cggcggcgtt gaccaggaag cgacgcgaca gcatcggcat
     2101 gcaccacccc ggcgcattgt gacagttccg ccgctctgga gtcaccggac ttttcgccgc
     2161 cctggccgtc gcggcaacaa acgatttctc ttgggaatag ttttatgact gtatgatgtt
     2221 cttgggagaa agacctggcg ggaaggaccc cgatggtcaa ggggaggagc tgatcatgga
     2281 gctcacgatc gtcaatcccc caccccggcc cgaagcgcac cagccgggca ccgacaaccg
     2341 ccagtgacag ccaacagcac agggagcaca gcgatgagca ccgcgtccac gaccccaccg
     2401 aacccacagt tcgccggcaa ggtcgccctg gtcgccgggg ccagccgcgg gatcggcgcc
     2461 gtcaccgcgc aggcgttcgc gcgggccggc gccgccgtgg tgcttgccgc ccgcgatcag
     2521 cgggcgctgg agcaggtcgc cgagagcatc cgcaacgacg gcggccaggc gctggtggtc
     2581 cccaccgacg tcggcgacgc cgcctcggtc gagcggctcg tcggccaggc cctcgacacc
     2641 ttcggccgcc tcgatgccgc cttcaacaac gccaccggcg gcccgctgcc ggcgcaactg
     2701 gccgagatcg accccgacga gttcgacacg ggcatccgcg tcaacgtccg cggcaccttc
     2761 ctgggcatga agtacgagat cccggcgctg ctggccagtg gcggcggcac ggtcgtgaac
     2821 atggcctcgg tcgccggtat ccaggccgtc gccaacctgg ccgcctacgc cgccggcaag
     2881 gccgggatca tcgccctcac caaggtcgcc gcgctcgact acgccgacca gggcgtccgc
     2941 gtgaacgtcg tcgcccccgg accgatcctg acctatcacc tggaggccgc cggcccggag
     3001 gcgcaacgcc aggccggcca gtccaccccg atgcgccgcg tcggccaggc cgaggaggtc
     3061 gccgccaccg tcctgtggct gtgctccgac cagtcgtcgt ttgtgaccgg cgcggtggtg
     3121 ccgatcgacg gcggccagtc cgccgggctg aagcccccgc ggatgtaccg ccagggccag
     3181 tcgatggcag ccgccaagcc gtaacgtaac cgcccgtatc agctcgcggg gcatgcggcc
     3241 ggcgacgtct ctgtcagccg gccgcatgct tgccttggtc gggccgtgtg cgaaactcgc
     3301 ccggtgacgc cgaagaccgg tccgctcgcg ggcggtgctg cgctggctcg gcctggccga
     3361 cgcggacggg ctgggctagc ggctgggctg cggcagctcg cagcggtcct tgaagccctg
     3421 tggagtcagg ccgagcgcga ggttgaaccg cgagcgcagg ttgtccatgc agacagccat
     3481 tgtcagctcg accagctgcg cctcgtcgaa gtgctcccgc aggcgcttga ccagctcgtc
     3541 gtcgaccgtc ggcggcgtct cggtcatcgc ctcggcgtac tccatgacca gccgctccag
     3601 cggattgaac aggtcgctgt cgcgccagcg cggcaccgcc gcgatcttct cgtgcgtgat
     3661 gccgtggccc agcagcaccc agtaaccgaa atccatgcac cacggacagc cgatcctggc
     3721 ggtcgcggcc agctcggcca ggtccttcag ctgctcgtcc atccggtgcc agcggggcac
     3781 ccgctgctcc agctggccga tcgccctcgc cacctgcttg tggtggccca tggcgcggaa
     3841 cgggtcgagg tcgtccccga acctccgccg gatgaaccag gcgccgatcc ggtacgacag
     3901 actccctggt ggatcaagcg agatgcgtgc catcttccgt ctccctgtca cattgaacgg
     3961 atttcgtcgg tcactgcttt gacccgacac cgccagggaa tgtgaccgaa gggctcaatg
     4021 aaccaacacg actggctggc agaacgcttc gaggccgacc gcggccacct gcgcgcggtc
     4081 gcctaccgca tgctcggctc gctcgccgag gccgacgacg ccgtccagga ggcctggctg
     4141 cggctcagcc gcgccgacac cagcgacgtc caaaacctca gcggctggct gaccacggtg
     4201 gtggcgcggg tgtgcctcaa cctgctgcgc gcccggagag cgcggcgcga ggagccgatc
     4261 gacgtggacg ggcagcgctc cgtccccgag ctggccgcgg gccgtgcgtc aggtgccgac
     4321 cccgagcagg aggcgctgct ggccgactcg gtcgggctcg cgctgctggt ggtgctggac
     4381 acgctgtccc cggccgagcg gctggcgttc gtgctgcacg acctgttcgc ggtaccgttc
     4441 gacgagatcg cccccatcct ggagcgctcc ccgaccgcgg cccgccagct cgccagccgc
     4501 gcccgccgcc gggtgcacgg ccacgacccg gaggcgctcc ccgacgccga cctccaccgc
     4561 cagcgggagg tcgtcgacgc cttcctcgct gcctcgcgtc acggcgactt cgaggcgctg
     4621 ctcgccgtgc tcgacccgga cgtcgtcctg cgcgccgacc gcagcgccgc ccataccgcg
     4681 acggcgaccg agatccgtgg cgccgcggcc gtcgcgaagg gagcgctcgc cttccaggga
     4741 cgctcccgct tcgcgcaact ggcactggtg cacggcgccg tgggcgtcgt cgtggccccg
     4801 cgcggccggc tgttcgtggt cctcggcctc acagtccggc gcggcaggat caccgcgatc
     4861 gacgtgatcg ccgaccccga gcggctgcgc cagctcgatc tcgcggtatt cgacgcctga
     4921 cgtcacgtta cgcggggtgc ggccgtccct tgtcagggcg tttcggcacg cccatgatga
     4981 gacgctgacg cgatagcccc ggtatccgat ccggggggat tgttcgatgg atcggaggcg
     5041 aggatgacgg aaccgttccc gttcagcgac ttcagcgact tcagcgacga ggatcctccc
     5101 atcgaggacg ccagcatcaa ccagcaccgc gccaccgatg acccacacag caaggtcttc
     5161 gacgaccgcg gcggcccggt cgaggacgtt accagcgtca gcggctggcc agaccccggt
     5221 ttcgaggacg tcgccttccc cagccctggc ggtggcgggt ccgggccggc tgggggggaa
     5281 cgatgaccgc gaccagcgtg atgacggtga tcgaccggct gcgggccatc gagcttggag
     5341 aagcgaagaa gagcgcggca tcggagcccg gcacggtccg caacccgttc gggtccaaca
     5401 acgtcaagta caacaccgcc ttctacggcc acgaggtcag cgacccggcg gggaagcagt
     5461 tcgcctggtg cgtcgtgttc cagtggtggt gcatgcaggc ggcgggggtt cccacgtcga
     5521 tcttcccgaa gtccgccggc gtgtccttcg tgcgggactg gttcgacgac ccgcaccgcc
     5581 gccgctactt ccgggtcccg accacgccgc aggtcggcga cctggtgatc ttcaagtaca
     5641 gccacatcgg cttcgtggag agcgtcgacc cgacctttgt cgtgacggtc gagggcaaca
     5701 gctccaaccg ggtccgccgc gtccagcacc gccgcgacga agccgagatc gacggctact
     5761 gccgtcccgc gtatgaccac gtccaggttc aggcgcaagg gggactttca atggcggacg
     5821 tcaacgacat cctggccaag ctcgaactgc tccgcgtcgg ggagcggaag gggacggcag
     5881 ccgaccccca tgacttcgcc tcgctcgagg gtgtcggcat gaaggttgac gccgcccgcg
     5941 ccgagatcga ctggctgatg gcagccgtca aggcaatcgc tgccaaggtc ggcgcgcaac
     6001 tccctccccc gccgcagtaa cccgagtggg atctccgctt ccggccagcg gcgtgcgtct
     6061 agaggtggct ggcggagaag gtgtcgcctc gccgcagcca gtctcggtgg cgccagtgac
     6121 caggtgggtc ggaaccctag tagccctgac aatcacggcg ggacgtgacc cgcgaccgcg
     6181 caggctcaac ctgatgatcg ccgccatcgc ctctac
//
LOCUS       DASWDC010000286         8161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1331860, whole genome shotgun sequence.
ACCESSION   DASWDC010000286 DASWDC010000000
VERSION     DASWDC010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331860"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /gene="lspA"
                     /locus_tag="VF995_09245"
     CDS             complement(<1..527)
                     /gene="lspA"
                     /locus_tag="VF995_09245"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX9343784.1"
                     /translation="MSPKSLRLAAMYGTAAVVVVADQASKYAVVRTLAERGPLHVLGS
                     FVEFRFQTNSGGAFSLFTGVPLFFALMAMVIIGGIVYAGTRARGTAVLLTLGLLLGGA
                     IGNLLDRALRGGVPLRGEVVDFVKVGRWPVFNLADSCITVGGLLLALLLSRAERDQHD
                     EGEGAQDEAARSTTS"
     gene            complement(573..956)
                     /locus_tag="VF995_09250"
     CDS             complement(573..956)
                     /locus_tag="VF995_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX9343785.1"
                     /translation="MAQNSPTTPDLDRLRERLEAELAELTGQRDDLEAASASDDAAGE
                     LGFAEEFADVGSYTFERERDQSLADNVRDLIDKVEAALARLRDGSYGRCEKCGQPIEP
                     ERLEALPYATLCLADARRRARLRAR"
     gene            1384..4596
                     /gene="ileS"
                     /locus_tag="VF995_09255"
     CDS             1384..4596
                     /gene="ileS"
                     /locus_tag="VF995_09255"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX9343786.1"
                     /translation="MGTFPEVDPKLDLPALDAAMLELWRERKVFERSLEQRHGAKGFV
                     FYEGPPTANGRPGVHHVEARVFKDLFPRFKTMRGYSVNRKGGWDCHGLPVELEIEKEL
                     GFTHKRQIEEYGVDRFNARCRESVLRYVDVWETLTERIGFWIDTSQAYRTMDTSYVES
                     VWWALAELHRRGLLVEDYKVVPYCPRCETALSDAEVSQGYQDVEDLTAYVVLRPTSGT
                     LAEEGAGLLVWTTQPWTLVPNVSVVVGPAIEYVLVEAERDGARRKLVVARDLMVQAVG
                     EDARVLRPVPLQELVGTRYERAFDLVPITEEQAGRGWRVVTDEYVTTTDGSGLVQTSP
                     AYGAEDLAVGQRYGTPILHPVGPDGRFGPQTGWLSGKDVKEADAEIVEDLRRRGLLWR
                     AEPHRHSYPHCWRCGRNLIYYALTSWYARTTAKKEVLLAENAKIGWHPEHIREGRFGD
                     WLAHNVDWALSRARYWGTPLPLWRCPQGHVTAVGSLADLSKKAGSDLSGLDPHRPYVD
                     EIVFACPEVDCGADARRVPDVLDAWFDSGSMPFAQWGYPHQGEVSFQREYPADFISEA
                     IDQTRGWFYSLLAVSTLLFDQTPYRNVVCLGHIVDRDGRKMSKSLGNVLDPFALLDQY
                     GADPLRWFMVASGSPWVARRLSGEALEEVTRSFFLTLWNTFSFFTLYARLEGFDPSEA
                     GAGAPSALDRWVLSELADTVRLVTERLDAYDPTAAARLLASFVDDLSNWYVRLSRRRF
                     WRGAGAAGSDGGEDGTAAFRTLWTCLRTLALLLAPFAPFTAEQLWQGLVVSVDPGAPD
                     SVHLADWPEPDEDALDPELDLAMSAVRELVVLGRTARTESKVKVRQPLARALVTVPAE
                     RRDDVAGLLDLVAAELNVKEVAFAESESGLVTFRLVPNFRALGPRFGKDAPGVAQALR
                     DVDAATAADLAPRLRAGERVTVWAHGLGPVEIGPEEATVTEEPVTGWRVAREGAFSVA
                     LDLDLTPDLRREGLARDLVRAVQDLRKSAGLAVSDRIDLAIKASGEVAEAVDAHEDYL
                     RSETLAVSLHRAPQGDGFDARVDLDGQEVRLWLRPVPATG"
     gene            4566..5018
                     /locus_tag="VF995_09260"
     CDS             4566..5018
                     /locus_tag="VF995_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343787.1"
                     /translation="MAPPSPRHRLSLRADGLGTLLPHRRPWWGYLWAAPNTLIGLLGA
                     ATTRTRPIPHRGVLLFEPTPGARPRGLARFLAWRGFEAITLGHAIITNTPLDDHVLAH
                     EFVHIRQHERWGPLFYPAYLLTSLAGYRRNPFERAADRASAAPLRRDR"
     gene            complement(5036..5491)
                     /locus_tag="VF995_09265"
     CDS             complement(5036..5491)
                     /locus_tag="VF995_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9343788.1"
                     /translation="MSVSARPLRIGEAAERAAVSVRTLRYYEELGLLTPSAHSPGGAR
                     RYAEDDLARLARIRELKDLLGFNLDEVRTVLTAEDRLDSLRAAYHAETNDRAAQRAIV
                     EEAIEINARLQAAVEAKRGLLATFQEALQTRATRYQAALANLQSPTPTA"
     gene            complement(5488..7194)
                     /locus_tag="VF995_09270"
     CDS             complement(5488..7194)
                     /locus_tag="VF995_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9343789.1"
                     /translation="MLVATINSSIVIISLPAIFRGIHLDPLRPGNVSYLLWMLIGYLV
                     VSAVLVVAFGRLGDIYGRVKMYNAGFLIFTVASVALGFTPNVGSAGALELIGLRIVQG
                     VGGALLMANSMAILTDAFPANERGMALGINQVAALAGSFIGLLVGGLLADTNWRAVFL
                     VSVPFGILGTVWGYLKLREGGQRGHGHGRIDWAGNLTFGVGLIAVLVGITYGIQPYGG
                     HTMGWTSPLVLTLLVGGVALLGLFLLVERRSSDPLFHLELFRVRAFSAGNLAGLVSSI
                     ARGGLQFMLIIWLQGIWLPLHGYSFASTPLWAGIYMVPLTLGFLLAGPVSGWLSDRHG
                     ARPFATGGMLLCAASFAALMLLPIDFPYLVFAALLLANGIGSGLFAAPNTAGIMNAVP
                     ARQRGAASGMRATFQNAGFVLSIGLFFSLMVAGLAARLPTTLYAGLTANGVPPAAATR
                     VAHLPPVGSLFAAFLGYNPVKTLLGPATLARLSPAQVANLTGKRFFPQLIAGPFRHGL
                     AIVFLAAAAMSLIAAAASWLRGGKYIHEEEPARPAAPAEATGDDEGENGDEAGSREEL
                     SA"
     gene            7386..8156
                     /locus_tag="VF995_09275"
     CDS             7386..8156
                     /locus_tag="VF995_09275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9343790.1"
                     /translation="MADMPVNPDPTTETTRIKGHNGDQIEAYLAAPAGPGPYPGVVVI
                     HHMPGFDEGTKGIARTFARHGYTAIVPNLHYREAPGASPDDASAAVRAAGGVPDDRLV
                     GDVAGAAALLRALGSGTGKVGVIGYCSGGRQAFLAACSLPLDAAVDCYGGFVVNPPPQ
                     DSPSKAKPVVGLAGSLSCPLLGLFGAEDTHPSPAETAQLEQELARHGKTYEFHTYKGA
                     GHAFFAVDRPGYRPEAAAAGWERIWDWFGRYLAAEPEG"
BASE COUNT         1197 a         2872 c         2964 g         1128 t
ORIGIN      
        1 tcactggtgg tgctgcgggc ggcctcgtcc tgggcgccct caccctcgtc atgctggtct
       61 cgctcggcgc ggctgagcag cagggcgagc agcaggccgc cgaccgtgat gcaggagtcc
      121 gccaggttga acaccggcca gcgcccgacc ttgacgaagt cgaccacctc gccgcgcagc
      181 ggcacgccgc cgcgcagcgc ccggtcgagc agattgccga tcgcgccgcc gagcagcagg
      241 ccgagggtga gcagcacggc ggtgccgcgg gcgcgggtgc cggcgtagac gatcccgccg
      301 atgatcacca tcgccatcag cgcgaagaac aacggcaccc cggtgaacag cgagaacgcc
      361 ccgccggagt tggtctggaa gcggaactcc acgaagctgc cgagcacgtg cagcggcccg
      421 cgctcggcga gcgttcggac caccgcgtac ttgctggcct ggtcggcgac gaccaccacg
      481 gcggcggtgc cgtacatggc ggcgaggcgg agactctttg ggctcacgag ggcgacgtcg
      541 ccttcaccgt gcggttcacc ttcaccgtcc cgtcaccgtg cccgcaggcg ggcccggcgg
      601 cgcgcgtcgg ccagacacag ggtggcgtag ggcagcgcct ccagccgttc cggctcgatc
      661 ggctggccgc atttctcgca gcggccatag ctgccgtcgc gcaggcgggc cagcgccgct
      721 tcgaccttgt cgatcaggtc gcggacgttg tcggccagcg actggtcccg ctcccgctcg
      781 aaggtatagg accccacgtc ggcgaactcc tcggcgaagc caagctcgcc cgcggcgtcg
      841 tcgctggcgc tggcggcctc caagtcgtca cgctggccgg tcagctcggc cagctccgcc
      901 tcaagccgct cgcgcagccg gtcaagatct ggtgtggtgg gtgaattctg tgccatcggc
      961 gtgctccttc aggatgcgac gcattgtagc gaccgggaag gccgaagggg tcccgcgttc
     1021 gctgggcgtg agcagcgcaa ccggcttgcc ggagttgtta cactggccct acaggcagtg
     1081 acgggaacga gtagaggggc ggcacggccc cagcgagccg gggacggtgg aagcccggcg
     1141 gcacggcgcc ctcgaagatc ctccccgagc cgctgcccga acggcgggac agcatcgacc
     1201 ggccctcccg cccagtagac gcagccgggt ccgcccgtga cagcgggagc cgttcgacat
     1261 gacggcgtga gtggtaccgg ccgcgaggcc ggtggcaaga ggggtggtac cgcggggccc
     1321 cggcctcgtc ccttcgcaaa ggtctcgatg actgcttgac cgtgcgaagg agagaggatt
     1381 ccgatgggga cgttcccgga ggttgacccg aagcttgacc tccccgccct cgacgccgcg
     1441 atgctggagc tgtggcggga gcgcaaggtg ttcgagcgtt ccctggagca gcgccacggc
     1501 gccaaggggt tcgtgttcta cgagggcccc ccgaccgcca acggccgccc cggcgtccac
     1561 cacgtcgagg cgcgggtctt caaggacctg ttcccgcgct tcaagaccat gcggggctac
     1621 tcggtcaacc gcaagggcgg ctgggactgc catggcctcc cggtcgagct ggagatcgag
     1681 aaggagctgg gcttcaccca caagcggcag atcgaggagt acggcgtcga ccggttcaac
     1741 gcccgctgcc gcgagtcggt gctccgctac gtggacgtct gggagacgct gaccgagcgg
     1801 atcggtttct ggatcgacac cagccaggcc taccggacca tggacacctc ctatgtggag
     1861 tcggtgtggt gggcgctggc ggaactgcac cgccgcgggc tgctggtgga ggactacaag
     1921 gtggtcccct actgtccgcg ctgcgagacc gccctgtcgg acgccgaggt gtcgcagggc
     1981 taccaggacg tcgaggacct gaccgcctac gtggtgctgc ggccgaccag tgggacgctg
     2041 gccgaggagg gcgccgggct gctggtgtgg accactcagc cgtggacgct ggtccccaac
     2101 gtctcggtgg tggtcggacc ggcgatcgag tatgtcctgg tggaggccga gcgcgacggt
     2161 gcccgccgga agctggtggt ggcccgtgac ctgatggtcc aggccgtcgg cgaggacgcg
     2221 cgggtgctgc ggccggtgcc gctccaggag ctggtcggga cccgctacga gcgcgccttc
     2281 gacctggtcc cgatcaccga ggagcaggcc gggcgtggtt ggcgggtggt gaccgacgag
     2341 tacgtcacca ccaccgacgg gtccgggctg gtccagacct ccccggccta cggcgccgag
     2401 gacctcgcgg tcgggcagcg ctatggcacc ccgatcctgc acccggtcgg gccggacggg
     2461 cgcttcgggc cgcagaccgg gtggctgtcg ggcaaggacg tgaaggaggc cgacgccgag
     2521 atcgtcgagg acctgcgccg gcgggggctg ctgtggcggg cggagccgca ccggcacagc
     2581 tacccgcact gctggcgctg cgggcggaac ctcatctact acgcgctgac ctcgtggtat
     2641 gcgcgcacca ccgcgaagaa ggaggtgctg ttggccgaga acgccaagat cggctggcac
     2701 ccggagcaca tccgtgaggg ccgtttcggc gactggctgg cccacaacgt cgactgggcg
     2761 ctgtcgcggg cgcgctactg gggcaccccc ctgccgctgt ggcgctgccc acaggggcac
     2821 gtgacggcgg tcgggagcct cgccgatctg tcgaagaagg cgggcagcga cctgtcgggc
     2881 ctggacccgc accggcccta cgtggacgag atcgtcttcg cctgcccgga ggtggactgc
     2941 ggcgcggacg cgcggcgggt gccggacgtg ctggacgcct ggttcgactc gggctcgatg
     3001 ccgttcgcgc agtggggcta cccgcaccaa ggcgaggtgt cgttccagcg ggagtacccg
     3061 gcggacttca tctccgaggc gatcgaccag acccggggat ggttctactc gctgctggcg
     3121 gtctcgacgc tgctgttcga ccagacgccg taccgcaacg tggtctgcct cgggcacatc
     3181 gtggaccgcg acggccgcaa gatgtccaag agcctcggca acgtgctgga cccgttcgcc
     3241 ctgctggacc agtacggcgc cgacccgctg cgctggttca tggtggccag cggctccccc
     3301 tgggtcgcgc gccgcctgtc gggcgaggcg ctggaggagg tcacccgttc attcttcctc
     3361 accctgtgga acacgttcag cttcttcacc ctgtacgcgc ggctggaagg gttcgacccg
     3421 tcggaagccg gtgcgggcgc gccgagcgcg cttgaccggt gggtgttgtc ggagctggcc
     3481 gacacggtgc ggctggtgac cgagcggctg gacgcctatg acccgaccgc cgccgcgcgg
     3541 ctgctggcaa gcttcgtgga cgacctgtcc aactggtacg tacggctgtc gcggcggcgg
     3601 ttctggcgtg gcgccggagc agctgggagc gacggcggcg aggacggcac ggcggcgttc
     3661 cgcacgctgt ggacctgcct gcggaccctc gcgctgctgc tggcgccgtt cgcgccgttc
     3721 accgccgagc agctgtggca gggcctggtg gtctcggtgg acccgggcgc cccggactcg
     3781 gtccacctgg ccgactggcc ggagccggac gaggacgccc ttgacccgga gctggacctc
     3841 gccatgtccg cggtccgcga actcgtggtg ctggggcgca ccgcccgcac cgagtccaag
     3901 gtcaaggtgc gccagccgct cgcccgcgcc ctggtcaccg tccccgccga gcggcgcgac
     3961 gacgtcgccg ggctgctcga cctggtcgcc gctgagctga atgtgaagga ggttgccttc
     4021 gccgagagcg agtcgggcct ggtcaccttc cgcctggtcc ccaacttccg cgccctcggc
     4081 ccccggttcg gcaaggacgc tccaggggtc gcccaggccc tccgcgacgt ggacgccgcc
     4141 accgccgccg acttggcccc ccgcctccgc gccggcgaac gcgtgaccgt ctgggcccac
     4201 ggcctcggcc cggtcgagat cggccccgag gaggccaccg tcaccgagga gcccgtcacc
     4261 ggctggcgcg tcgcccgcga gggcgccttc agcgtcgccc tcgacctcga cctgaccccc
     4321 gatctgcgcc gcgagggcct cgcccgcgac ctggtccgcg ccgtccagga cctccgcaag
     4381 agtgccggcc tcgccgtcag tgaccgcatc gacctggcca tcaaagcgag tggggaggtc
     4441 gccgaagccg tcgacgccca cgaggactac ctccgctccg aaaccctcgc cgtctccctg
     4501 caccgcgccc ctcagggcga cggcttcgac gcccgcgtcg acctcgacgg ccaggaggtc
     4561 cgcctatggc tccgcccagt ccccgccacc ggctgagctt gagggcggac ggcctcggca
     4621 ccctcctccc ccatcgccgc ccctggtggg gctacctgtg ggctgccccc aacaccctta
     4681 tcggcctgct gggggcagcc accacccgca cccgcccgat cccccaccgt ggcgtcctgc
     4741 tgttcgaacc caccccagga gcccgccccc gcggcctcgc ccgcttcctc gcctggcgcg
     4801 gcttcgaagc catcaccctg ggccacgcga tcatcaccaa cacccctctg gacgaccacg
     4861 tccttgccca cgagttcgtg cacatccgcc agcacgagcg ctggggtccg ctgttctatc
     4921 ccgcatattt gctgaccagc ctggccgggt accgtcgcaa cccgttcgaa cgcgcggcgg
     4981 accgggcgag cgccgcgccg ctgcgccgcg atcgctgacc gcaccggcgg ctgcctcagg
     5041 ccgtgggcgt tggggattgg aggttggcca gggccgcttg gtagcgcgtg gctctggtct
     5101 gcagcgcttc ctggaaggtg gcgaggaggc cacgcttggc ctcgacggcg gcttggaggc
     5161 gggcgttgat ctcgatggcc tcctcgacga tggcgcgctg ggcggcgcgg tcgttggtct
     5221 cggcgtggta ggcggcgcgg agggaatcga ggcggtcttc ggcggtgagg acggtgcgga
     5281 cctcgtcgag gttgaagccg aggaggtctt tgagttcgcg gatgcgggcg aggcgggcga
     5341 ggtcgtcctc ggcgtagcgg cgggcaccgc cagggctgtg ggccgacggg gtgagcaggc
     5401 cgagttcctc gtagtagcgc agggtacgga ccgagacggc agcgcgttcc gcagcctccc
     5461 cgatgcgcaa cggccgcgcg ctcacgctca cgccgacagc tcctcccgcg accccgcctc
     5521 gtctccgttc tcgccctcgt cgtcgccagt ggcctcggca ggcgcggccg ggcgggcggg
     5581 ctcctcctca tgaatgtact tgccgccgcg cagccaggag gcagccgcgg cgatcagcga
     5641 catcgcggcg gcggcgagga acacgatcgc cagcccgtgc cggaacggcc cggcgatgag
     5701 ctggggaaag aaccgcttgc cggtgaggtt ggcgacctgc gccgggctca ggcgcgcgag
     5761 cgtggccggg ccgagcagcg tcttgaccgg gttgtagccg aggaaggcgg cgaacaggct
     5821 gccgaccggc ggcaggtggg cgaccctggt ggccgcggcc ggcggcacgc cgttggcggt
     5881 cagcccagcg tacagcgtgg tcggcaggcg ggcggccagg ccggcgacca tcagggagaa
     5941 gaacagcccg atcgacagca cgaacccggc gttctggaag gtggcgcgca tgccggaggc
     6001 ggcgccgcgc tggcgtgctg ggacggcgtt catgatcccg gcggtgttcg gcgcggcgaa
     6061 caggcccgag ccgatgccgt tggccagcag cagcgcggcg aacaccaggt aggggaagtc
     6121 gatcggcagc agcatgaggg cggcgaagct ggcggcgcac agcagcatgc cgccggtggc
     6181 gaacggccgc gcgccgtgac ggtccgacag ccagccggac accggtccgg cgagcaggaa
     6241 cccgagggtg agcgggacca tgtagatccc cgcccacagc ggcgtcgagg cgaaactgta
     6301 gccgtgcagc ggcagccaga tgccctgcag ccagatgatc agcatgaact ggaggccgcc
     6361 gcgggcgatc gaggagacca gcccggcgag gttgccggcc gagaaggcgc ggacccggaa
     6421 cagctccagg tggaacagcg ggtcgctgga gcgccgctcc accagcagga acaggccgag
     6481 cagcgcgacc ccgccgacga gcagggtgag caccagcggg ctggtccagc ccatggtgtg
     6541 gccgccgtag ggttggatgc cgtaggtgat gccgaccagc acggcgatca gcccgacgcc
     6601 gaaggtgagg ttgccggccc agtcgatccg accgtgaccg tggccgcgct gccccccctc
     6661 gcggagcttg aggtagcccc agaccgtccc gaggatgccg aacgggacgc tgaccaggaa
     6721 caccgcccgc cagttggtgt cggccagcag tcccccgacg agcaggccga tgaaggagcc
     6781 ggccagcgcc gccacctggt tgatgccgag cgccatgcca cgctcgttgg ctgggaaggc
     6841 gtcggtgagg atggccatgg agttggccat cagcagggcg ccgccgaccc cttggacaat
     6901 ccgcagcccg atcaactcaa gggcgccggc ggagccgacg ttgggcgtga acccgagcgc
     6961 gaccgaggcg acggtgaaga tcaggaaccc cgcgttgtac atcttgaccc ggccgtagat
     7021 gtcgccgagg cgcccgaagg cgaccaccag cacggcgctg accaccaggt agcctatcag
     7081 catccacagc aggtagctga cgttgccggg ccgcagcggg tcgaggtgga tgccgcggaa
     7141 gatcgccggc agggagatga tcacgatcga cgagttgatg gtggcgacca gcatccccag
     7201 ggtcgtgttc gacagggcga tccacttgta gcggtcgtcc cgctcccccg ccgcccggct
     7261 cggttccggg cggctaccag ctttcgcacg cagcattgtt gaatcctcat gctcgacggc
     7321 tacaaaacct tacgttcacg taagcatcat agccgcaacg gcggccgcga cgaggaggac
     7381 aggcgatggc cgacatgccg gtcaacccgg acccaacgac ggagacgacc cggatcaagg
     7441 ggcacaacgg ggaccagatc gaggcctacc tggccgcccc cgccggcccc ggcccctacc
     7501 ccggggtggt cgtgatccac cacatgcccg gcttcgacga gggcaccaag ggaatcgccc
     7561 ggaccttcgc ccgccatggc tacaccgcga tcgtgccgaa cctccactac cgtgaggcgc
     7621 cgggcgccag ccccgacgac gcctcggcgg cggtccgggc ggccggcggg gtgcccgacg
     7681 accgactggt cggcgacgtc gccggggccg cggcgttgct gcgagcgctg ggctcgggga
     7741 ccggcaaggt cggcgtgatc ggctactgct cgggagggcg gcaggcgttc ctggccgcct
     7801 gcagcctgcc gctcgacgcg gcggtcgact gctacggcgg cttcgtcgtc aacccgccgc
     7861 cgcaggactc gccgagcaag gccaagccgg tcgtcggact ggccggcagc ctctcgtgcc
     7921 cgctgctcgg cctgttcggc gccgaggaca cccacccgtc cccggccgag accgcccagc
     7981 tcgaacagga gcttgctcgc catggcaaga cctacgagtt ccacacctac aagggcgcgg
     8041 gccacgcctt cttcgccgtc gaccggccgg gctaccgccc cgaggcggcc gctgccggct
     8101 gggagcggat ctgggactgg ttcggccgct acctggccgc cgagccggag ggctgagcag
     8161 a
//
LOCUS       DASWDC010000287         5643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_580548, whole genome shotgun sequence.
ACCESSION   DASWDC010000287 DASWDC010000000
VERSION     DASWDC010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580548"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1044)
                     /locus_tag="VF995_09280"
     CDS             complement(22..1044)
                     /locus_tag="VF995_09280"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HEX9343791.1"
                     /translation="MQSADEDALMEIRARAPLRISFAGGGTDVPPYPATHGGAVLSAT
                     IDRWAVAGVRVRDDRAYHVESLDLMVRKAFQPHELVFDGHLDLVKAVLRDLAPEQGLD
                     LTLGTDAPPGSGLGSSSALVVAMLAAVGATSGRNWSPAELAWRAYQVERIDLKIQGGM
                     QDQYAAAFGGLNFIEFHGDDNVAVRPLALAPETLEDLEGSLLLAWSGAGRTDGGILRR
                     QVDGVLGGSARSLSSLAAIKALAEAMRDALLGGDLATFAEGLHEGWEQKRRLASGIAT
                     PRLDELYQVARDAGAAGGKVPGAGGGGFLLFCAPPGALRRVAAALEAAGAACTPVRFV
                     PQGAHW"
     gene            complement(1044..2171)
                     /locus_tag="VF995_09285"
     CDS             complement(1044..2171)
                     /locus_tag="VF995_09285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9343792.1"
                     /translation="MNLLLLTNDFPPRAGGIQQYCHNLVSRLDPASVTVYAPDWPGAA
                     AFDAAQPFRVMRHPTSRMLPTADLARRVIALVREVHPDVICFGASVPLGLLAGRVTRA
                     TGTPCIGFSHGVEAAVGRVPLVRRLLAKVAGDLRLVTAVSRWSAARVERGVRGRCPVE
                     LLPSGVATGSYHPGVDGGAIRARHGLGDAPVCVCVSRLVPRKGQDRLIQAWPAVAARV
                     PDARLLLVGGGPDERRLRRLAAASPASDRIHLTGEVSWEELPAHYATGDVFAMPCRTR
                     WLGLDLEALGAVFLEAAAAGLPVVAGRSGGAPETVVEGETGIVVDGRRAEPVGQAVAE
                     LLADPPRASAMGEAGRRFVEARYAWDAVVARFEKLLVEAAS"
     gene            complement(2255..3496)
                     /locus_tag="VF995_09290"
     CDS             complement(2255..3496)
                     /locus_tag="VF995_09290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9343793.1"
                     /translation="MSPPRHARLRRRVPRPRPGDPLEAFSPDEIAACLAYAKPRQRWA
                     MVAFGVELALLAALALSAPGRALVRGTIGLAGHFTAGQAALGVAVVVLVQALASLPFS
                     LRNWSQDVSAGLATQRLGGFLLDWVKSRAVGLALTVVPTGALILAARWRPPGWPWAAG
                     MVAALLVVALSLLGPVVLEPLFNRFRPLPPGPLRERVVTLAATMGIGVREVLVADASR
                     RTTKVNAYVSGLGRTRRVVVYDTLLARSTAAPGGTSEDEVALVLAHELAHVRHRDMLW
                     GTTAAAAGAAAGVVFANWLLARPWPRHWLHASGLGDPNVAAGLLLLGALGGLLISPLA
                     SAVSRFAEARADWTALEVSHDPVTAVAVERRLALENRADLRPNRMMVALFATHPPIMA
                     RIAQARLWASSHGVGEATTLS"
     gene            complement(3615..5171)
                     /locus_tag="VF995_09295"
     CDS             complement(3615..5171)
                     /locus_tag="VF995_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343794.1"
                     /translation="MNPSDEGSPPRIPEWIPGRVAEPTPPGWRSPSPPSPAAPPGRPP
                     AAAAPAPPPAPPRAPLPPDAPSWLRQAPTRAPDAPVVWGPPQAPRRRPRGWLIALVVV
                     GLALALVAAVLSLQSPDAAPSRVASSVAPRLRANDPVRQVQHVLALRAQAIVHHNRAG
                     FLASVDSRRTSFYRAQAVLFDRMATAPFSGFAYQLDSPANLATARIRQRYAPAPVYLP
                     EIQASYHFRGQDASPVLARFYYTFVWTGSAWRIAAQDELRPLVQSDAEIWDAGTLQTV
                     ATSRTLVVFHPGDSVLANRLRGAAEQGYAQVAASWSGSWEHKVVILVPHDQAEAERLV
                     GARDLGQVAAVAASSIESGAFQRVLGNRIVVNSTNVRRYDQLNLQVVMTHEMTHVATR
                     TIGQGVPLFIVEGFADFTALRPIGQPLAFTRPALASAVKAGRFDGTLPSDADLRGANA
                     SVAYDASSSFCLWVATTFGEPRLQALYRAFRGTAEPGIAGQDIVFRHVLGISRATAQT
                     RWATWVRQVL"
     gene            complement(5322..>5643)
                     /locus_tag="VF995_09300"
     CDS             complement(5322..>5643)
                     /locus_tag="VF995_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX9343795.1"
                     /translation="VLAQLGKPYHWGGAGPSSFDCSGLTMYSWAKAGVSMPHSAAAQY
                     DLFPHVARSALQAGDLVFFGSPIHHVGLYIGQNLMVAAPSTGRLVQVQTLVGRSDYVG
                     AARP"
BASE COUNT          803 a         2024 c         2166 g          650 t
ORIGIN      
        1 tccgatatca atcacgctga atcaccaatg cgctccttgg gggacgaagc ggacaggggt
       61 gcaggcagcg ccggccgcct ccagcgcggc ggcgacgcgc cggagggcgc cgggcggtgc
      121 gcagaacaac aggaacccgc ccccgccggc gccggggacc ttgccgccgg ccgcgccggc
      181 gtcgcgggcg acctggtaca gctcgtcgag gcgcggggtg gcgatgccgc tggcgaggcg
      241 gcgcttctgc tcccagccct catgcaggcc ttcggcaaag gtcgcgaggt cgccgccgag
      301 cagcgcgtcg cgcatcgcct cggcgagcgc cttgatcgcg gcgaggcttg agaggctgcg
      361 cgccgagccg ccgaggaccc cgtcgacctg ccggcgcagg atgccgccgt cggtgcggcc
      421 cgcccccgac caggccagca gcagcgagcc ctccaggtcc tccagggtct ccggcgcgag
      481 cgccaggggc cgcaccgcca cgttgtcgtc gccgtggaac tcgatgaagt tgaggccgcc
      541 aaaggcggcg gcgtactgat cctgcatgcc gccctggatc ttgaggtcga tccgctcgac
      601 ctggtacgcg cgccaggcca gctccgcggg cgaccagttg cggccgctgg tggcgccgac
      661 cgcggcgagc atcgccacca ccagcgccga cgaggagccg agcccggagc ccggcggcgc
      721 gtcggtgcca agcgtcaggt cgaggccttg ctcgggggcg aggtcacgca ggaccgcctt
      781 gaccaggtcc aggtgcccgt cgaagaccag ttcgtgcggc tggaatgcct tgcggaccat
      841 gaggtcgagc gactcgacgt ggtaggcgcg gtcgtcgcgg acccgcacgc cggcgaccgc
      901 ccagcggtcg atggtggccg acaggaccgc gccgccgtgg gtggccggat agggcgggac
      961 gtcggtgccg ccgccggcga agctgatccg cagcggcgcg cgggcgcgga tctccatcag
     1021 cgcatcctca tcagctgact gcatcagctg gccgcctcga ccagcagctt ctcgaaccgg
     1081 gcgacgaccg cgtcccaggc gtagcgggcc tcgacgaagc ggcgtccggc ctcccccatc
     1141 gcgctggcgc ggggcgggtc ggcgagcagc tcggccacgg cctgcccgac cggttcggca
     1201 cggcggccgt cgaccacgat gccggtctcg ccctcgacga cggtttcagg cgcgccaccg
     1261 gaccggcccg ccaccaccgg cagcccggcg gcggcggcct ccaggaagac cgcgccgagc
     1321 gcctccaggt ccaggccgag ccagcgggtg cggcacggca tcgcgaacac gtccccggtg
     1381 gcgtagtggg ccggcagttc ctcccaggac acctcgccgg tcaggtggat gcggtcgctg
     1441 gccgggctgg ccgcggcgag ccggcgcagg cgccgctcgt cgggaccacc gccgaccagc
     1501 agcaggcggg cgtcggggac ccgggcggcc accgccggcc atgcctggat gagccggtcc
     1561 tggcccttgc gtgggaccag gcgggagacg cagacgcaca ccggcgcgtc gccgaggccg
     1621 tggcgggcac ggatcgcgcc gccgtcgacc ccgggatggt agctgccggt ggcgaccccg
     1681 gagggcagca gctcgaccgg gcagcggccc cgcacgccgc gctccacccg cgccgccgac
     1741 cagcggctca cggcggtgac cagccgcagg tcgccggcga ctttggcgag cagtcgccgc
     1801 accagcggca cgcgcccgac ggcggcctcg acgccgtggc tgaagccgat gcacggcgtc
     1861 ccggtcgccc gggtgacgcg gcccgcgagc aggccgagcg ggacgctcgc cccgaagcag
     1921 atcacgtcgg ggtggacctc gcggaccagc gcgatcacgc ggcgggcgag gtcggcggtg
     1981 ggcagcatcc gactggtcgg gtggcgcatc acccggaacg gctgcgcggc gtcgaaggcg
     2041 gcggcaccgg gccagtccgg cgcgtagacc gtcaccgagg cggggtcgag tcgactgacc
     2101 aagttgtggc agtactgctg gatcccccca gcgcgcgggg gaaagtcatt ggtcagcaat
     2161 aggagattca caaactgatc atagaggctg ccgcctggct cctgccggag cggcggcgcc
     2221 gattggcagg ttcggtcgcg ctctcagtcg cagctcagga cagggtggtc gcctcaccga
     2281 cgccgtggct ggaggcccac aggcgggcct gggcgatgcg cgccataatc ggcgggtgcg
     2341 tggcgaacag cgccaccatc atccgattgg gacgcaggtc ggcgcggttc tccagggcga
     2401 ggcggcgttc caccgcgacc gcggtgaccg ggtcatggga gacttccagg gcggtccagt
     2461 cggccctggc ttcggcgaag cggctcaccg cgcttgccag ggggctgatg agcaggccgc
     2521 cgagggcgcc gagcagcagc agcccggctg ccacgttcgg gtcaccgagg ccgctggcgt
     2581 gcagccagtg ccgcggccac ggccgggcca ggagccagtt ggcgaagacc accccggcgg
     2641 ccgcgccggc tgctgcggcg gtggtccccc acagcatgtc gcggtgccgc acgtgggcca
     2701 gctcgtgcgc gagcaccagc gcgacctcgt cctcggaggt gccgccgggt gcggcggtgg
     2761 acctggcgag cagggtgtcg tacaccacca cccggcgggt gcggccgagg ccggagacat
     2821 aggcgttgac cttggtggtg cggcggctcg cgtcggcgac cagcacctcg cggacaccga
     2881 tccccatggt cgccgcgagg gtgacgaccc gttcgcgcag cggccccggg ggcagcggcc
     2941 ggaaacggtt gaatagcggc tcaagcacca ccggcccgag caggctgagc gccaccacca
     3001 gcagcgccgc gaccatgccg gccgcccatg gccagccggg cggccgccag cgcgccgcca
     3061 ggatcagcgc gccagtcggc accaccgtga gcgccagccc gaccgcgcgg ctcttcaccc
     3121 agtcgagcag gaagccgccg aggcgctggg tggcaaggcc ggcgctgacg tcctgtgacc
     3181 agttgcggag cgagaacggc agcgaggcga gcgcctgcac cagcaccacc acggcgaccc
     3241 cgagcgccgc ctgcccggcg gtgaagtggc cggccagtcc gatcgtcccg cgcaccagcg
     3301 cccggccggg cgcgctcagc gcgagcgcgg ccagcagcgc gagctcaacc ccgaacgcga
     3361 ccatggccca gcgctgccgc ggcttggcgt aggcgaggca ggcagcgatc tcgtcgggcg
     3421 agaacgcctc cagcgggtcg ccaggccggg gacgcgggac ccgccggcgc agccgggcgt
     3481 gacgcggcgg actcaccctg gcctcatccg gcacgccccg ggatcgtcat ccgccgcggc
     3541 aacgcactca ccgtggacca atccggcacg ccccgggatc gtcgtccgcc gcggcaacgc
     3601 gctcaccgtg gacctcatag gacctgccgc acccaggtgg cccagcgggt ctgggcggtt
     3661 gcccgggaga tgccgagcac gtggcggaag acgatgtcct ggccggcgat gcccggctca
     3721 gcggtgcccc ggaaggcccg gtacagcgcc tgcaaacggg gctcgccgaa ggtggtggcg
     3781 acccatagac agaacgacga gctggcgtcg taggcgacgg aggcgttcgc gccgcgcagg
     3841 tcggcgtcgg acggcagggt gccgtcgaag cggccggcct tgacggcgga ggccagcgcc
     3901 gggcgcgtga acgcgagcgg ctgcccgatg ggccgcaggg cggtgaagtc ggcgaagccc
     3961 tccacgatga acagcggcac gccctgcccg atcgtgcggg tggcgacgtg ggtcatctca
     4021 tgggtcatca ccacctgcag gttgagctgg tcgtagcggc gcacgttggt gctgttgacc
     4081 acgatgcggt tgccgagcac ccgctggaac gcgcccgatt cgatcgagga ggcggccacc
     4141 gccgcgacct gtccgaggtc gcgggcgccg accagccgtt ccgcctccgc ctggtcgtgt
     4201 gggaccagga tcaccacctt gtgctcccag gagcccgacc aggaggccgc gacctgcgcg
     4261 tagccctgct cggcggcgcc ccggaggcgg ttggcgagca cgctgtcgcc ggggtggaag
     4321 accaccagcg tgcggctcgt cgcgacggtc tgcagcgtcc cggcatccca gatctccgcg
     4381 tcgctttgga ccagcgggcg cagctcgtcc tgggcggcga tgcgccaggc cgagccggtc
     4441 cagacgaagg tgtagtagaa gcgcgccagg accgggctcg cgtcctggcc gcggaagtgg
     4501 tagctggcct ggatttcggg caggtagacc ggtgcggggg cgtagcgctg ccggattcgg
     4561 gcggtggcca ggttggcggg gctgtcgagc tggtaggcga agcccgagaa gggcgccgtc
     4621 gccatgcggt cgaacagcac cgcctgggcg cggtagaagc tggtccggcg cgagtcgacg
     4681 ctggcgagga agccagctcg gttgtggtgc acgatcgcct gtgcccgcag cgcgaggacg
     4741 tgctgcacct gccggacggg atcgttcgcc cggaggcgcg gcgccacgct ggaggcgacc
     4801 cgtgacggcg cggcgtccgg cgactgcagc gagagcacgg ccgcgaccag cgcgagcgcc
     4861 agcccgacca cgacgagggc gatcagccac cccctcggcc gtcgccgcgg cgcctgcggc
     4921 ggcccccaga ccaccggcgc gtccggggcc cgcgtcggcg cctgccggag ccagctcggc
     4981 gcgtcgggag gcagcggcgc ccgcggcggc gccggcggcg gcgcgggcgc cgccgcggca
     5041 ggcggcctgc ccggcggtgc cgctggtgaa ggtggggacg gcgagcgcca gccgggtggg
     5101 gtcggctcgg caacccgccc agggatccac tcggggatcc gtggggggga gccttcgtcg
     5161 cttgggttca tgcgccgcat cgtaaccgac cccagtcact cgcaacggtg gttatccaca
     5221 gcccccgtca accgccgtcc cccacactcc cagaaagagc tacggccctc gcccggggga
     5281 tagcgagggc cgtagcggct tgagagcgac gggggctcag ctcagggacg ggcggcgccc
     5341 acgtagtcgc tcctgccgac gagggtctgg acctggacga gccggccggt ggacggcgcg
     5401 gccaccatca ggttctggcc gatgtacagg ccgacgtggt ggatggggct gccgaagaag
     5461 accaggtcgc cggcctgcag ggcgcttctg gccacgtggg ggaacaggtc gtactgcgcc
     5521 gcggccgagt gcggcatcga gactccggcc ttggcccacg agtacatggt cagcccagag
     5581 cagtcgaacg acgacggccc ggccccaccc cagtggtagg gcttgccgag ctgcgcgagc
     5641 acg
//
LOCUS       DASWDC010000288         1919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_330782, whole genome shotgun sequence.
ACCESSION   DASWDC010000288 DASWDC010000000
VERSION     DASWDC010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330782"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VF995_09305"
     CDS             complement(<1..690)
                     /locus_tag="VF995_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343796.1"
                     /translation="MAGTHADQDPGHTTPLAPEDLDWVPQPRPRPKRRGKRALWAVGL
                     VVVLIAGTGFAVAQRSHRGRAAAPPGIAAGLRWSRVGGTSAILPTAIIAGGPGFIAGS
                     WATPLSGAPLWTSPDGANWSQVPVTDQQFGSFRPEAMVSFGGRIFAAGPQQTGQRARF
                     GVWSSTDGQIWSPAVTPAALWTASDSVMLDGHPGLGLVVSALTAGPRGLVAVGYHSDT
                     HPDRSQPLAWSS"
     gene            complement(806..>1919)
                     /locus_tag="VF995_09310"
     CDS             complement(806..>1919)
                     /locus_tag="VF995_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343797.1"
                     /translation="SIEAGRPVALGLVAARGLDGNEAARNHQVVAHAYEEDPESGAFR
                     MHVYDPDRPGEQVVLGFEPKDPRLVEAVEAAASGAGGRTGGVEGRCWRGCFVQDYLPE
                     QPAYVDLAISKGIWVSAWTPLVGQHFVAQFTARNYGDHPARLAELCLALRGPGGERLD
                     GAFTAAAEAEAEAPEQVGALALELEPGAERALVRHTNSFGAVPGGYRITAAYRSEGGH
                     LRALPPGEPGTVSQVGVTAVAPLWPTLVRVRFLRVTVDDAAAIGGRCALELVVNGQTS
                     RWPAEGTVGCEDGETLDLDVRFEVALGPAEPLTVTVTGPCGRLRGHWLGLDGWGQGAH
                     RCRSRLRDDSPDPVDGAYTVAFTIEASTAEAEAEEA"
BASE COUNT          272 a          752 c          664 g          231 t
ORIGIN      
        1 cgaagaccag gccagcggct gcgaccggtc cggatgcgtg tccgaatggt agccaacagc
       61 gaccagaccc ctcgggcccg ccgtcaacgc cgacacgacc agccccagcc cggggtggcc
      121 atcgagcatg acgctgtccg aggccgtcca gagcgccgcc ggcgtcacag ccggcgacca
      181 gatctggccg tcggtggacg accagacgcc gaaccgggcc cgctggccgg tctgctgcgg
      241 acccgccgcg aagatccggc cgccgaagga gaccatcgcc tccggacgga acgagccgaa
      301 ctgctggtcc gtcaccggca cctggctcca gttcgcaccg tccggcgagg tccagagcgg
      361 tgccccgctg agcggcgtcg cccaggaccc ggcgatgaac cccgggccgc cggcgatgat
      421 cgcggtcgga aggatcgcgc tggtaccccc gacccgcgac cagcgcagcc cagcggcgat
      481 ccccggcggc gcggctgccc gcccacggtg ggaacgctgc gccacggcga accccgtgcc
      541 cgcgatcagc accacgacca gcccaaccgc ccacagagcc cgcttcccac ggcgtttggg
      601 ccgagggcga ggttgtggca cccagtcgag gtcctccggg gcgaggggtg tggtgtggcc
      661 aggatcttgg tcggcgtggg tgcccgccat cggggtctcc gggttccttg gttgcggcag
      721 caggcgaatt ccagcaggta gatacaaatg ccgtcaatcg cggtgccata tcgagcaacc
      781 cgcggtccgc ctcccgacgg cctcgctacg cctcctcggc ctcggcctcg gccgtgctcg
      841 cctcgatggt gaaggcgacc gtgtaggcgc cgtcgaccgg gtccgggctg tcgtcgcgga
      901 ggcggctgcg gcagcgatgg gccccctggc cccagccgtc gaggccgagc cagtgcccgc
      961 gcaggcgccc gcagggcccg gtgaccgtga ccgtgagcgg ctcggccggg ccgagcgcca
     1021 cctcgaagcg gacgtcaagg tcgagggtct cgccgtcctc gcagccgacc gtgccctccg
     1081 ccggccagcg gctggtctgg ccgttgacga ccagctccag cgcgcaacgg ccgccgatcg
     1141 cggcggcgtc gtccacagtg acccgcagaa accgcacccg caccagcgtc ggccacagcg
     1201 gcgcgaccgc cgtcaccccg acctgcgaga ccgtgcccgg ctcgccgggc ggcagcgccc
     1261 gcaggtgccc gccctcggaa cggtaggccg cggtgatccg gtagccgccc ggaaccgcgc
     1321 cgaagctgtt ggtgtgcctg accagcgccc gctccgcgcc cggttccagc tccagcgcca
     1381 acgcgcccac ctgctccgga gcctcggcct cggcctcggc ggcggcggtg aaggcgccat
     1441 ccagccgctc cccgcccggg ccgcgcagcg ccaggcacag ctcggcgagg cgagccgggt
     1501 ggtcgccgta gttcctggcg gtgaactgcg ccacgaaatg ctggccgacc agcggcgtcc
     1561 aggcgctcac ccagatcccc ttcgagatcg ccaggtcgac gtaggcgggc tgctcgggca
     1621 ggtagtcctg cacgaaacag ccccgccagc agcgaccctc gacgccgccc gtcctccccc
     1681 cggcaccgga ggcagcggcc tcgacggctt cgaccaggcg cggatccttc ggttcgaagc
     1741 cgagcaccac ctgctcgcca ggccggtccg ggtcgtagac atgcatgcgg aacgcgccgc
     1801 tttccgggtc ctcctcatag gcgtgggcga ccacctgatg gttgcgggcc gcctcgttcc
     1861 cgtcgagccc gcgggcggcg accaacccga gcgccaccgg ccgccccgcc tcgatactg
//
LOCUS       DASWDC010000289         4149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_735531, whole genome shotgun sequence.
ACCESSION   DASWDC010000289 DASWDC010000000
VERSION     DASWDC010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735531"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VF995_09315"
     CDS             complement(<1..1234)
                     /locus_tag="VF995_09315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9343798.1"
                     /translation="MSLTASTSTLSIQEHSFADLGATPATLTSLARIGIEHPFPIQTL
                     CLPLALSGVDLIGQARTGTGKTLAFGIPIVQDIDPGEPVVQALVVVPTRELCLQVASD
                     LENAGRTAGVRVTAIVGGRPLGPQIVALNEGAHVVVGTPGRLLDHLRQGSLSLKQVRA
                     LVLDEADEMLDLGFLPDVETIIEATPQHRQTMLFSATMPKEVVALARRYAKQPTFVHA
                     EPGALATVPATRQYFFQVHPLNRFEVLCRLIDSPHRDRVAVFRRTKRGVERTIQGLKE
                     RGYRAGALHGDLPQAIREQVMNKFRKGKLDVLVCTDVAARGLDIAGLSHVINYDTPED
                     ERAYVHRIGRTGRAGAAGVAITFVAWNEADTATLIRERLGLTDEPVQEVYSTSDLLVE
                     LFDLPTVEAASAQRAAATP"
     gene            1439..1780
                     /locus_tag="VF995_09320"
     CDS             1439..1780
                     /locus_tag="VF995_09320"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9343799.1"
                     /translation="MASPADVYQALREAHGTADAESAAALYAPDAEYYEPDSEVHRGR
                     DEIRTFLAAYFARRGPVGISVKREVVADSSVLAEWTSSYTEAGRRWAGTPGVSVLEVG
                     DDGITYHRDYQ"
     gene            complement(1806..2036)
                     /locus_tag="VF995_09325"
     CDS             complement(1806..2036)
                     /locus_tag="VF995_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HEX9343800.1"
                     /translation="MEVKLGVTYSPKELVVEIDDSSDSVVRAVDQVITGDARMLWLVD
                     AKGRRIGVPTDKLAYIEIGEDNPSKRVGFASL"
     gene            2245..3129
                     /locus_tag="VF995_09330"
     CDS             2245..3129
                     /locus_tag="VF995_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343801.1"
                     /translation="MYRRALVIIGCLALLAAGCGQAAPPATKGGGPAQAPTYAAKGQV
                     GTPTRNRDTLYLATPGAATTQVAAVAPASRAVLRASTIPGRWALPAIGLAKVPDGLSG
                     DGSTLVLQEVGGSHTISRFAVLDSAMRKPAQVAVLHGWFDYDAVSPDGGTLYLIEHLA
                     ANDLLHYRVRSYEVANRQLDEAIVVDKRELDEASMQGLPNTRVSDGGWAWTLYRSPKG
                     PFIHALDLGQKFAVCIDLPAKARSSDRVARLWTLTYDEPSGELHAANTALHLAVRVQF
                     NENDPFGYRVATGELTTN"
     gene            3253..>4149
                     /locus_tag="VF995_09335"
     CDS             3253..>4149
                     /locus_tag="VF995_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX9343802.1"
                     /translation="MGEARMMGGTVVAVAVALLAGAAAGVWLMRRGGRSGGGTALERR
                     LEGMSQDLDRRLAALDQRLWLGLDTVQNAQAQAAESAADVRERVAVVGGIAERVLEHA
                     RDLARLEELLRPPQARGAFGEVLLEQLLAQGLPGACYRTQHQFRSGARVDAVLQLGDT
                     LVPVDSKFPLDSFARMVACADGDEARASHRRAFIRDARRHLDAIAEKYIRPDEGTLDF
                     ALCYLPSEAVYYEFLREDPPGESPFRYAVNCRVFPVSPGSLYAYLLSIGVGLRGLRVE
                     ANARRVVDALATLQGDLGRFADD"
BASE COUNT          589 a         1450 c         1499 g          611 t
ORIGIN      
        1 cgggcgtcgc cgcggcgcgc tgggcgctgg ccgcctccac cgtgggcagg tcaaaaagct
       61 ccaccagcag gtcgctggtc gagtagacct cctggacggg ctcgtcggtg aggccgaggc
      121 gctcgcggat cagcgtggcg gtgtcggcct cgttccaggc gacgaaggtg atcgccaccc
      181 cggcggcgcc ggcacggccg gtgcggccga tgcggtgcac gtaggcgcgc tcgtcctcgg
      241 gggtgtcgta gttgatcacg tgcgagaggc cggcgatgtc caggccgcgg gcggcgacgt
      301 cggtgcagac cagcacgtcg agcttgccct tgcggaactt gttcatgacc tgctcgcgga
      361 tcgcctgggg caggtcgccg tgcagcgcac cggcgcggta gccgcgctcc ttgaggccct
      421 ggatggtccg ctcgaccccg cgcttggtgc gccggaagac cgccacccgg tcgcggtgcg
      481 gcgagtcgat cagccggcac agcacctcga agcggttcag cgggtggacc tggaagaagt
      541 actgccgcgt cgccggcacg gtggccaggg cgccgggctc ggcgtgcacg aaggtgggct
      601 gcttggcgta ccggcgcgcc agcgccacca cctccttcgg catggtggcc gagaacagca
      661 tcgtctggcg gtgctgcggg gtcgcctcga tgatcgtctc gacgtccggc aggaagccga
      721 ggtcgagcat ctcgtcggcc tcgtccagca ccagggcgcg gacctgcttc agcgagaggc
      781 tgccctggcg caggtggtcg agcagccgcc cgggggttcc caccacgacg tgggcgccct
      841 cgttcagcgc gacgatctgg gggccgagcg ggcggccgcc cacgatcgcg gtgacccgca
      901 cgccggcggt gcggccggcg ttctcaaggt cgctcgcaac ctggaggcag agttctctgg
      961 tagggaccac gaccagcgcc tggaccacgg gttcgcccgg gtcgatgtcc tgcacgatcg
     1021 ggatgccaaa ggcgagggtc ttgccggtgc cggtgcgcgc ctggccgatc aggtcgacgc
     1081 cgctcagcgc tagcggcagg cagagggtct ggatggggaa ggggtgctcg atgccgatcc
     1141 gggccagcga cgtgagcgtc gccggggtcg caccgaggtc ggcgaagctg tgctcctgga
     1201 tggaaagggt tgatgtcgat gcggtcagac tgatcctcca aaatcatgga cgtccggggc
     1261 gaacgtacgg aaaaaggcag agcttcgacg atgctccgac cacgagtgta gtccagctgt
     1321 agtcaagctt ggccagcccc cttatcgacc accccggcca cggccttagc cgagaccttg
     1381 gcgagcgggc ccattgcggt agtcttcagc ggaatcatcg ccgtccagga ggttacccgt
     1441 ggccagtccg gccgacgtct accaggcgct ccgtgaagct catgggaccg cggacgcgga
     1501 gagcgccgcc gcgctgtacg cacccgacgc ggaatattac gagccggaca gcgaggtcca
     1561 tcgcggccgc gacgagatcc gcaccttcct ggccgcctat ttcgcccgcc gcggcccggt
     1621 cggcatcagc gtcaagcgcg aagtggtcgc cgactcctcg gtgctggcgg aatggaccag
     1681 ctcctatacc gaggcgggcc gccgctgggc aggcacgccg ggggtgtcgg tgctggaggt
     1741 cggcgacgac ggcatcacct accaccgcga ctaccagtag gcggtcggcg gcgtccagcg
     1801 ggcgtctaca ggctggcgaa gccgacccgc ttgctggggt tgtcctcgcc gatctcgatg
     1861 taggcgagct tgtcggtcgg cacgccgatc cggcggccct tggcgtcgac cagccagagc
     1921 atgcgcgcgt ccccggtgat cacctgatcg actgcccgga ccaccgagtc ggacgagtcg
     1981 tcgatctcca ccaccagctc cttcgggctg taggtgacgc ccagcttgac ctccatggtg
     2041 ctgtttcctc ctgtcggggc gccgccaggg cggcggtggt tcgagtgggt tggttttatc
     2101 cagcttgagg ttgcgggcgc gagcgtagca ccgccgcggg caatggaacc gaccacgcgg
     2161 gcccggtgta tcagagccga cgacaacgcc actggcgaac gccactggcg aacgccactg
     2221 gcgaacgcac tgggggtgga cgggatgtat cgccgtgctc tggtcatcat cgggtgcctg
     2281 gcgctgctgg ccgccggctg cggccaggcg gcgccgccag ccacgaaggg aggagggccg
     2341 gcccaggcgc ccacctacgc ggccaagggc caggtcggca cgccgacccg gaaccgggac
     2401 accctgtatc tcgcgacgcc cggggcggcc acgacccagg tggcggcggt cgcgcccgcc
     2461 agccgcgcgg tgctgcgcgc cagcacgatc ccgggacgct gggccctccc ggcgatcggg
     2521 ctcgccaagg tccctgacgg gctctccggc gacggctcca cgctggtcct gcaggaggtc
     2581 ggcggcagcc acacgatcag ccgcttcgcg gtgctcgaca gcgccatgcg caagcccgcc
     2641 caggtggcgg tgctgcacgg ctggttcgac tacgacgcgg tctccccgga cggcggcacc
     2701 ctctacctga tcgagcacct cgccgccaac gacctgcttc actaccgggt ccgctcctat
     2761 gaggtggcga accggcagct ggacgaagcg attgtcgtcg acaaacgtga actggacgag
     2821 gcctcgatgc agggcctgcc gaatacccgc gtcagcgacg gcggctgggc ctggaccctg
     2881 taccgcagcc ccaaggggcc gttcatccat gcccttgacc ttggccagaa gttcgcggtc
     2941 tgcatcgacc tgcccgcgaa ggcgcgctcc agcgaccgcg tcgcccgact ctggacgctc
     3001 acctacgacg agcccagcgg cgagctgcat gcggccaaca ccgccctgca cctggccgtc
     3061 cgggtccagt tcaacgagaa cgaccccttc gggtaccggg tcgccaccgg cgaattgacc
     3121 accaattgag ttgtggaggg tgcggcgccg gccagttggc ttcggtcgct gtcgcaccct
     3181 cctgatagcg tccgcgccat cgccgggcgg ccccgctgaa ggttcccagg aggggcgacc
     3241 ggcgctcatc tcatggggga ggcgcggatg atgggcggga cggtggtggc ggtcgcggtc
     3301 gcgctgctgg cgggcgcggc cgctggcgtg tggctgatgc gccggggcgg cagaagcggc
     3361 gggggaacgg cgctggagcg gcggctggag ggcatgtcgc aggacctcga ccggcgcctg
     3421 gcagcgctcg accagcggct gtggctcggg ctggacacgg tgcagaacgc ccaagcgcag
     3481 gcggccgagt ccgccgccga cgtacgcgaa cgggtggcgg tggtcggcgg catcgccgag
     3541 cgggtcctgg agcacgcccg cgacctcgcc cggctggagg agctgctgcg cccgccgcag
     3601 gcccgtggcg ccttcggcga ggtgctgctg gagcaactgc tcgcccaggg gcttcccggc
     3661 gcctgctacc gcacccagca ccagttccgt tccggggccc gggtggacgc ggtgctgcag
     3721 ctcggcgaca ccctggtccc ggtcgactcc aagttcccgc tggactcgtt cgcacgcatg
     3781 gtggcctgcg ccgacggcga cgaggcccgc gcctcgcacc gccgcgcctt catccgggac
     3841 gcgcgccgcc acctcgacgc gatcgccgag aagtacatcc gccccgacga gggaactctc
     3901 gacttcgccc tgtgctacct gccctccgaa gcggtctact acgagttcct gcgcgaggac
     3961 ccgccgggcg agtcaccctt ccgctacgcg gtcaactgcc gcgtcttccc cgtgtcgccg
     4021 ggcagcctct acgcctacct gctctccatc ggcgtgggcc tgcgcggcct ccgggtcgag
     4081 gcgaacgccc gccgggtggt cgacgcgctg gccaccctcc agggcgacct cggccgcttc
     4141 gccgacgat
//
LOCUS       DASWDC010000290        10832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1248658, whole genome shotgun sequence.
ACCESSION   DASWDC010000290 DASWDC010000000
VERSION     DASWDC010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10832
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248658"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..621)
                     /locus_tag="VF995_09340"
     CDS             complement(97..621)
                     /locus_tag="VF995_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9343803.1"
                     /translation="MTASAADWRDPGLLEVGRGTVERAGIDYNGHLNVAHYRAAFDLG
                     TDELFAHLGLGAEDYNPSANATLMVIEDHTRYHAELRLGESYRILAQLVGHSAKTIRY
                     LMFMENLDRGGLAATHEELALHVDLAGRRASPLPAEVLPALSALEADHARLPLHPDLG
                     RSVGQPAGQRSAPY"
     gene            complement(676..1141)
                     /gene="bcp"
                     /locus_tag="VF995_09345"
                     /pseudo
     CDS             complement(676..1141)
                     /gene="bcp"
                     /locus_tag="VF995_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
     gene            complement(1243..2070)
                     /locus_tag="VF995_09350"
     CDS             complement(1243..2070)
                     /locus_tag="VF995_09350"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9343804.1"
                     /translation="MRNRAGAALTTLFAVALTLAVAACTGARAGGGDPSATTRARGSA
                     APATTAAPSTRAPATHTTPATPAAKPKPAPKPAPELRLGSRGAAVAALQRRLLALGYQ
                     APAADGQFGSETSHAVVAFQKVNRLSRDGVAGPVTLGALTHPRLPSPRVRTNGFHVEA
                     DLTPQVVYLVQDGRVTRVLDASSGSGRAYSENGDSGVAITPLGNFQVQHKINGWHKSK
                     LGLLYRPAFFDGGFALHGAPSVPPFPASHGCIRITIAAMDQLYDHLPVGTPVLVYRS"
     gene            complement(2184..3887)
                     /locus_tag="VF995_09355"
     CDS             complement(2184..3887)
                     /locus_tag="VF995_09355"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9343805.1"
                     /translation="MGFFQQPPELGSTYRSDVALREHLERLLPPDVLLEVVPELEALG
                     AAAATGGELARLAEEAEACPPRLVPFDPWGRRIDRVEVSAAWTALHAVQARAGLAAIP
                     HEGRHGPHARVVQHALLHLYGPSSAVYSCPVAMTDAAVRVLRDQAPPALRDRVVPRLL
                     SRDPERAWTSGQWMTERVGGSDLSRHATVARLDPTADPAQDGAWRLSGTKWFTSAVTA
                     DCALVLARPQWSDGASVPGSGGLGLFLVERVHPRTGVSQVGETILVNRLKDKLGTRAL
                     PTAELTLAGAAATPVGTVEAGVRKMAGMLNVTRVHNAVSAAAGMRRGTDLAFAYAQVR
                     EAFGHPLAELPLHRATLAALAVEAEAAFALVATVMERLGRVEHGAGGGAAGGAEREQR
                     ALRALVPLAKLLTAKDAVAHASEVVEAFGGAGYIEDSGIPRLLRDAQVLPIWEGTTNV
                     LSLDLLRAQRRDEAVTALLDELREGSAVLGGPHLGAVAALVEGTATDLAKDVEGWPSS
                     EEDELQARLRAFALRLGRTCAAASLAVHAAHRLARHGDTRAAKVAVSYATRWLAPGMP
                     Q"
     gene            4072..5484
                     /locus_tag="VF995_09360"
     CDS             4072..5484
                     /locus_tag="VF995_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9343806.1"
                     /translation="MPASAWPSGWLSRLRGALPRGRSLPDEVWRPRQRAILAILWLHV
                     AGLAALALAHGGNPEALAGVGLVALVAVPASTRYGSRRWRSALASLGLLLASVLLVRL
                     EGGAIELQFHFFVVIALIMLYQDGFLFLIAVGFVVAYYGAVGWLEAAGTYPPPVAWAN
                     PWQRAAVDEALLLAAAVANLASWRLNEDSQARAESSYRQLYEGQRAVVRELQQSQHLK
                     DELYNVVSHELRTPLTGILGFGELLLVRDGQLSPAQRKDHLGRILREARRLQRVVDNL
                     VYSSKVIRQDGGAATDVGAVVHAVIEDLDDLPGRDRVELEVRVPAGLQATLTPEAFRL
                     VLVNLLGNALKYATPGTTVFVSAWIEVGAGAGRAGDRLAVAVSNAGQPIPPADRERIF
                     QPFVQLDSSTTRTASGVGLGLHIVRRLVESFGGTVEVDSDVAGMVTFTVRVPMASRRS
                     DPARLGGSPSSHLRSSGGSI"
     gene            5561..6256
                     /locus_tag="VF995_09365"
     CDS             5561..6256
                     /locus_tag="VF995_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343807.1"
                     /translation="MTQADVSTAASTESTGSGSSSSSSSSSNSRSSVTGSGSRSAQAD
                     LDRSRSAAFSGGSLQLGELEEVDVRTVFQRDGDGFSGWLAGNLDRLANELGVNGLREV
                     GRDIGVGSFTVDILAQTDRGRRVAITSQLEAADHLHLGQVVTFAAGLDISAVIWICTK
                     ISEEHRAVLDWLNQHSDDDVRFFGVEMRLFRIGSSLPAAVFDVEARPNDWQKVVRQRQ
                     RVGSPFNSRMREF"
     gene            complement(6357..6758)
                     /locus_tag="VF995_09370"
     CDS             complement(6357..6758)
                     /locus_tag="VF995_09370"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX9343808.1"
                     /translation="MPAFEAVRRWRGLAVLDNDGNKIGSIVEIYYDSETDEPGWALLS
                     TAGIAGDTCLVPLRDAAEQGNEIRVPYDQLQVRGAPGMEPGGRLWPQDEAALYAYYAL
                     PYSGGLDDLDDEEDIGGTVTVGPSSRWHFGS"
     gene            complement(6962..7549)
                     /locus_tag="VF995_09375"
     CDS             complement(6962..7549)
                     /locus_tag="VF995_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX9343809.1"
                     /translation="MPVRPDDLRMAAWRSLLEAHAAVVELLEQELERACGLPLSQYEV
                     LLKLAGAPGGRLRMRELARSVLLSKSGLSRLVDRMAAAGLVCRERSLDDRRGAFAVLT
                     DQGRMALRKAAPVHLRGIQEHFAGYLGEAEAEVVGVALGRVTAAAREAGGQARGGSCE
                     PEPCEPDGQPEPCEPGGAAQDVGEAAQSGRVLSAD"
     gene            7707..8864
                     /locus_tag="VF995_09380"
     CDS             7707..8864
                     /locus_tag="VF995_09380"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9343810.1"
                     /translation="MSRDAMQLIKADRKKLESLFKQYEKTSAGDLEARERLVSEMTQQ
                     VSKIVSLKAQTVYPPMQAKLSRRGREMVLQAVEQQQIAKRMLGELSKMRPQDARFNAK
                     VHVLMENLRQHLKFEESKLMPQAKKTLSSRTRKEMGKQMEKETKMQPAKPAAKASAKA
                     ATKPAAKRTTASVSKTRSVSKAKPAARKSASTRPAAKPMSKTAKPAAKRTMKSAAKPA
                     AKRTASTSRTMKSAAKPAAKRTTAKATTRSMAKPAAKRTTSSRTTSRTVKSAAKPAAK
                     RTTSRTTAKSMSKSMAKPAAKRTTAKAATRSMAKPAAKRTVKVSSRSTAKPVAKSSAK
                     PTAKRMVKRTTAKAAARPAMRKTAMAARKTARPTAKRTTRRTGTRKLARVA"
     gene            9023..10666
                     /locus_tag="VF995_09385"
     CDS             9023..10666
                     /locus_tag="VF995_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9343811.1"
                     /translation="MRVLGVNGVFHDPAAALVVDGRIVAAAEEERFSRRKHGKPAVPF
                     STWELPERAAAWCLAEAGLAPEDLDAVAYSYDPGLAPSGGGDVTADGWEGLRTLYARR
                     APLFLRSVLPGLDPACIRFVPHHVAHAASAYLAAPWPDSSVLVLDGRGERASHLAGRA
                     AGGSLEILRAQALPHSLGILYEEVTAHLGFTRASDEYKVMALAAYGKPAFLREFREAV
                     RADGDGGFRVAPIELDTLAKRRASEAEELAEEHADLAASLQTRLEEVLLELAGWLHRQ
                     TGDSTLTMAGGVALNCVANSRLAAEGPFERIWVQPAAGDAGTALGAALWVAAAGGDQV
                     EPMRTAALGPGWGDDALEAWLVRANLPYERPADLADAVARALAADAVVAWFQGRSEYG
                     PRALGHRSLLANPAHAANLERLNDVKGREQFRPVAPMVLAERAPAIFEGPLPSPYMLF
                     THRVRPAWRERIPAVVHVDGSARIQTVDRAEEPLVARLLDAVERRTGVPVAVNTSLNT
                     AGRPMVDDPRDALECFGSAPVDVLALGPFLVRRANFARP"
BASE COUNT         1560 a         3866 c         3907 g         1499 t
ORIGIN      
        1 agacagccac caggcgagcc acgagtcaag tcaagcggat ggccgccccg aaaggtgcgg
       61 ccatccgtgc gtggaccagc cggaactccc gttaggttag tagggagcgg agcgctgccc
      121 cgccggctgc ccgaccgagc ggccgaggtc gggatggagc ggcaggcggg cgtggtctgc
      181 ttcgagggcc gacagcgccg gcagcacctc ggcgggcagc gggctcgcgc ggcggccggc
      241 cagatcgacg tgcagggcca gctcctcgtg ggtggcggcc agcccgcccc ggtcgaggtt
      301 ctccatgaac atcaggtagc ggatggtctt ggcgctgtgg ccgacgagct gggcgaggat
      361 ccggtaggac tcgccgaggc gcagctcagc gtggtaacga gtgtggtcct cgatcaccat
      421 cagggtggcg ttggcgctcg ggttgtagtc ctcggcgccc aggccgagat gggcgaacag
      481 ctcgtcggtg cccaggtcga aggctgcccg gtagtgcgcc acgttgaggt gcccgttgta
      541 gtcgatgccc gcccgctcga ccgttccccg cccgacctcc agcaggcccg ggtcgcgcca
      601 gtccgccgca ctcgccgtca tcaccgcagc ttcgcgcatc cacaactcga aggccaaacg
      661 gcgctgtcgc gggcgctatt cacccagttc cgcgaggacc ttggcggcgt ggccgtcggc
      721 cttgacgctg taccaggcgc gggcgacgct gccgtccccg tcgatcagga aggtggagcg
      781 gagcacgccg acggtggtcc tgccgtagag gttcttctcg ccccaggcgc cgtaggcctc
      841 cagaacccgg tggccggggt ccgacagcaa cgggaaggcg agcccgtact tggcacggaa
      901 gcgctggtgg ctggcggcgt cgtcgggcga gatcccgagc accggcacct cggcgcgctg
      961 aaaggcgtgc aggttgtcat tgaactggca ggcttccctg gtgcaccccg ggggtgtcgt
     1021 cggctgggta gaagtagacg atcacccggc ggcccttgaa gtcgtccagc gacacggtgt
     1081 tgccatcctg gtcgggcagg gagaacggcg gggcgggcgc gccggcgtcc agcttgggca
     1141 tggaggggtc ctcggttcgc agcggtggtc ggacccgccc agggtacctc caccgggtgg
     1201 ggcgaccccc tcggcgtcgg ccggtcccgt ccggtcgtcc ggtcagctcc ggtagacgag
     1261 caccggggtg ccgaccggaa ggtggtcgta gagctggtcc atggccgcga tggtgatgcg
     1321 gatgcagccg tggctggcgg ggaagggcgg cacgctcggg gcgccgtgca gggcgaagcc
     1381 gccgtcgaag aaggccgggc ggtacagcag cccgagcttg ctcttgtgcc agccgttgat
     1441 cttgtgctgg acctggaagt tcccgagcgg ggtgatggcc acgccgctgt cgccgttctc
     1501 gctgtaggcg cggccggagc cggaggaggc gtcgaggacg cgcgtgaccc tcccgtcctg
     1561 gaccaggtag acgacctgcg gggtgaggtc ggcctcgacg tggaacccat tggtccgcac
     1621 gcgcgggctg ggcagcctgg gatgcgtgag cgcgccgagg gtgaccggcc cggcgacccc
     1681 gtcgcgggac aggcggttga ccttctggaa ggcgaccacc gcgtggctgg tctcggagcc
     1741 gaactgcccg tcggcggcgg gcgcctggta gccgagcgcc agcagccgcc gctgcagcgc
     1801 cgccacggcg gcgccccgcg agccgagccg cagctcgggc gcgggcttcg gcgccggctt
     1861 cggcttggcc gccggcgtgg cgggggtcgt gtgcgttgcc ggtgcccggg tggagggcgc
     1921 ggccgtggtc gccggcgcgg cgctgccccg cgccctggtc gtggcactcg ggtcaccacc
     1981 gccagcccgc gccccggtgc aggccgcgac ggcgagcgtc agcgccacgg cgaacagggt
     2041 tgtcagcgcc gctccggcac ggttccgcat ctcgtctccc atcctcgaag gccaccaccg
     2101 cgtcgaggtc ctatctaccc agtgtgccga gaacgggaaa gtcttacagc cacgccgccg
     2161 taacaatcct ggcggacccg tctctattgg ggcattcccg gagcgagcca gcgggtggcg
     2221 tagctgacgg ccaccttggc ggcacgagtg tcgccgtggc gggcgagccg gtgggcggcg
     2281 tggacggcca gcgacgcggc ggcgcaggtg cgtcccagcc gcagcgcgaa ggcgcggagg
     2341 cgggcctgga gctcgtcctc ctcgctggag ggccagccct cgacgtcctt ggcgaggtcg
     2401 gtggccgtgc cctccaccag cgcggcgacg gctccgaggt gcgggccgcc cagcaccgcc
     2461 gacccttcgc gcagctcgtc gagcagcgcg gtgaccgcct catcgcggcg ctgcgcgcgc
     2521 agcaggtcga ggctgagcac gttggtggtg ccctcccaga tcggcaggac ctgggcgtcg
     2581 cgcagcaggc gcgggatgcc gctgtcctcg atgtagccgg caccgccgaa ggcctcgacc
     2641 acctcgctgg catgggcgac ggcgtccttg gcggtcagga gcttggccag tgggaccagc
     2701 gcccgcagcg cccgctgctc gcgctcagcg ccgcccgcgg cgccgcctcc ggcgccgtgc
     2761 tcgacccggc cgagccgctc catcaccgtc gcgaccagcg cgaacgcggc ctccgcctcg
     2821 accgcgaggg cggcgagggt ggcacggtgc agcggcagct cggcgagcgg atggccgaag
     2881 gcctcgcgca cctgggcgta ggcgaacgcc aggtcggtac cgcgccgcat cccggcggcg
     2941 gcgctgaccg cgttgtggac ccgggtcacg ttcagcatcc cggccatctt gcggacgccc
     3001 gcctcgacgg tcccgaccgg ggtggcggcg gcgccggcaa gcgtcagctc ggcggtcggc
     3061 agcgcccgcg tgccgagctt gtccttcagc cggttgacca ggatggtctc ccccacctgc
     3121 gagacaccgg tgcgcgggtg gacccgctcc accaggaaca ggccgaggcc accgctgcca
     3181 ggcaccgagg cgccgtcgga ccactgcggc cgggcgagca ccagggcaca gtcggcggtc
     3241 accgccgagg tgaaccactt ggtcccggag agccgccagg cgccgtcctg ggcagggtcc
     3301 gcggtggggt cgagccgggc gacggtggcg tggcgggaca ggtcggagcc gcccacccgc
     3361 tcggtcatcc actggcccga ggtccaggcg cgctcggggt cccgcgacag caggcgcggg
     3421 accacccggt cgcgcagggc gggcggcgcc tggtcgcgca gcacccgcac ggccgcgtcg
     3481 gtcatcgcca ccgggcagct gtagaccgcc gacgacggcc cgtagagatg cagcagcgca
     3541 tgctgcacca cccgcgcgtg gggcccgtgc cgcccctcgt gcgggatggc ggcgagcccg
     3601 gcgcgcgcct gcacggcgtg cagcgccgtc caggcggcgc tcacctcgac ccggtcgatc
     3661 ctgcggcccc acgggtcgaa ggggaccagg cgaggcgggc acgcctccgc ctcctcggcc
     3721 aggcgggcca gctccccgcc ggtcgcggcc gccgcgccca gcgcctccag ttccggcacg
     3781 acctcgagga gcacgtccgg gggcagcagc cgctccaggt gctctcgcaa ggccacgtcc
     3841 gaccggtagg tgctgcccag ctcgggcggc tgctggaaga agcccacgac ccgctcccct
     3901 catccggagt ccacgcggaa tccacgccaa caaccaaact acttccgacc aaacaaagtc
     3961 ttggcggcgg aacggggcga acacaaccac ctggggcagg cttgcgcagg caaaacttgt
     4021 tagtcttgca acacttgaga ctcttggtct gattcacctg aggagctacg tgtgcccgcg
     4081 tccgcatggc cgtcggggtg gttgagccgc ctccgcggcg cccttccccg gggtcgctcg
     4141 ctgccggacg aggtgtggcg ccccaggcag cgcgcgatcc tggcgatcct gtggctgcac
     4201 gtggccgggc tggcggcgct ggcgctggca cacgggggca accccgaggc gctggccggg
     4261 gtgggcctgg tcgcgctggt ggccgtcccc gcgagcaccc gctacgggag ccgccgctgg
     4321 cgctcggcac tggccagcct cgggctgctg ctcgcctcgg tgctgctggt gcggctggag
     4381 ggcggcgcga tcgagctgca gttccatttc ttcgtggtga tcgcgctgat catgctctac
     4441 caggacggct tcctgttcct gatcgcggtc ggcttcgtgg tggcctacta cggcgcggtc
     4501 ggttggctgg aggcggccgg cacctacccg ccgccggtcg cgtgggccaa cccctggcag
     4561 cgggccgccg tcgacgaggc gctgctgctg gcggcggcgg tcgccaacct ggcgagctgg
     4621 cgcctcaacg aggacagcca ggccagggcg gagtcctcct accgccagct ctacgaaggc
     4681 cagcgcgcgg tcgtgcgcga gctgcagcag tcccagcacc tcaaggacga gctctacaac
     4741 gtcgtctcgc acgagctgcg cacccccctc accggcatcc tcgggttcgg cgagctgctg
     4801 ctggtccgcg acgggcagct ctcaccggcc cagcgcaagg accatctcgg ccgcatcctg
     4861 cgggaggcac ggcgactgca gcgggtggtc gacaacctgg tgtactcctc caaagtgatc
     4921 cgccaggacg gcggcgcggc caccgacgtc ggcgccgtcg tccacgcggt gatcgaggac
     4981 ctggacgacc tccccggccg ggaccgggtc gagctggagg tccgcgtccc cgccggcctg
     5041 caggcgaccc tgacgcccga ggcgttccgg ctggtgctgg tgaacctgct cggcaacgcg
     5101 ctgaagtacg ccaccccggg caccaccgtg ttcgtgagcg cctggatcga ggttggcgcc
     5161 ggtgccggcc gcgccggcga ccgcctcgcg gtggcggtgt ccaacgccgg gcagccgatc
     5221 ccgccggccg accgggagcg gatcttccag ccgttcgtgc agctcgactc ctccaccacc
     5281 cggaccgcct cgggggtggg gctcgggctg cacatcgtgc gccgcctggt ggagtcgttc
     5341 ggcggcaccg tcgaggtcga ctccgacgtc gccggcatgg tgaccttcac cgtccgggtg
     5401 ccgatggcca gccgccgcag cgacccggcc cggctgggcg gctcgccctc atcacattta
     5461 cgcagttcgg gcggctcgat ttgactcgac ctgtgtcttt ttagaaccct tccagggtca
     5521 tgctgcgaga gccgcactac gaccagggaa aggatccagc gtgactcagg cagacgtcag
     5581 tacggccgcc agcacggaga gcaccggctc gggctccagt tccagctcca gctcaagctc
     5641 gaattcgcgc tcctccgtca cgggtagcgg cagccggtcc gcgcaggcgg acctcgaccg
     5701 gtccaggagc gcggcgttca gcggaggctc cctccagctc ggcgaactgg aggaggtcga
     5761 cgtccggacg gtcttccagc gggacggaga cgggttctcc ggctggctcg ccggcaacct
     5821 cgaccggctc gccaacgagc tcggcgtgaa cggcctgcgc gaagtgggcc gcgacatcgg
     5881 cgtcggctcg ttcaccgtcg acatcctggc ccagaccgac cgtggccggc gcgtcgccat
     5941 caccagccag ctcgaggccg ccgaccacct ccacctcggc caggtggtca ccttcgccgc
     6001 cggcctggac atctccgccg tcatctggat ctgcaccaag atcagcgagg agcaccgcgc
     6061 cgtgctcgac tggctgaacc agcacagcga cgacgacgtc cgcttcttcg gcgtcgagat
     6121 gcgcctgttc cgcatcggca gctccctccc ggcggcggtc ttcgacgtcg aggcccgccc
     6181 gaacgactgg cagaaggtcg tccgccagcg ccagcgcgtc ggcagcccct tcaactccag
     6241 aatgcgagag ttctaagcag cgtccggcgc agggggaccc gcgcgggtcc ccctgcgccg
     6301 gtccccgacc ggtgatgaag ctggtgctgg cgcccgccga gccgcggccg ggctgcctag
     6361 ctgccgaagt gccagcggct ggacgggccg acggtgacgg tgccgccgat gtcctcctcg
     6421 tcgtcgaggt cgtcgaggcc gccgctgtac ggcagcgcgt agtaggcgta gagggcggcc
     6481 tcgtcctgtg gccagaggcg gccgccgggt tccatgccgg gagcgccgcg tacctggagc
     6541 tggtcgtacg ggacgcggat ctcgttgccc tgctcggccg cgtcacgaag cgggacgagg
     6601 caggtgtcgc cggcgatgcc ggccgtgctc agcagtgccc atccaggctc atcggtctcg
     6661 gagtcatagt agatctcgac gatcgagcca attttgttgc cgtcgttgtc aaggacggcc
     6721 agacccctcc acctgcgcac ggcctcaaat gccggcatca cggcccccct cgcgttctgg
     6781 tgcctgcccc tgactggagc agcacgcgac tatccccacg ccggatgtta ccgaggagcg
     6841 gcgggcgatg gacagtctac tttgaggacc atgaccgaat agcgcgtgta ttgtctccaa
     6901 gagttgcgat cgttgcgcaa cacccagggc gcagttcact accatcgcgc cattaatcgg
     6961 gctaatcggc cgacaacacc cgcccacttt gtgcggcttc gcccacgtcc tgggccgccc
     7021 ctccgggttc gcacggctca ggctgcccgt caggctcgca cggctccggc tcacaggacc
     7081 cgccgcgcgc ttgccctccc gcctccctcg cggcggcggt gaccctgccg agcgcgacgc
     7141 cgaccacctc cgcctcggcc tcgccgaggt agccggcgaa gtgctcctgg atgccacgca
     7201 ggtggacggg cgcggccttc cgcagcgcca tccggccctg gtcggtgagc accgcgaagg
     7261 cgccgcgccg gtcgtccaga ctccgctccc ggcacaccag cccggcggcg gccatccggt
     7321 cgaccagccg cgacagccca ctcttggaga gcagcaccga gcgcgccagc tcccgcatcc
     7381 ggaggcgccc gccgggcgcg cccgccagct tcagcagcac ctcgtactgg ctgagcggca
     7441 gcccgcacgc gcgctccagc tcctgttcca gcagctcgac cacggcggca tgcgcctcca
     7501 gcaacgagcg ccaggcggcc atccgaaggt cgtcaggtcg tacaggcatg cgccaatagt
     7561 agagacacgc tccagaccac gttcgggctg tcttagcgtg gcaatcgggc gagttttggg
     7621 gcgcatccga atttagggtt caagtttttg gcggaggcat accgatgaat agcccgatcc
     7681 cctcaaagga cttgcaagga ggcaatgtgt cgagggatgc aatgcagctc atcaaggccg
     7741 accgcaagaa gctggaaagc ctcttcaagc agtacgagaa gaccagcgcg ggcgacctgg
     7801 aggcgcgcga gcgcctggtc agcgagatga cccagcaggt cagcaagatc gtctcgctca
     7861 aagcgcagac cgtctacccg ccgatgcagg cgaagctcag ccggcgtgga cgcgaaatgg
     7921 tcctgcaggc cgtcgagcag cagcagatcg ccaagcgcat gctcggcgag ctgtccaaga
     7981 tgcgcccaca ggacgcgcgc ttcaacgcca aagtccacgt gctgatggag aacctccgcc
     8041 agcacctcaa gttcgaggag tcaaagctca tgccgcaggc caagaagacg ctcagcagcc
     8101 gcacccgcaa ggagatgggc aagcagatgg agaaggagac caagatgcag cccgccaagc
     8161 cggccgccaa ggcgtccgcc aaggccgcga ccaagccagc cgccaagcgc accaccgcct
     8221 cggtcagcaa gacccggtcg gtcagcaagg ccaagccggc ggcgcgcaag tccgccagca
     8281 cccggcctgc cgccaagccg atgagcaaga cggccaagcc ggccgccaag cgcaccatga
     8341 agtccgccgc gaagccggcc gcgaagcgga ccgccagcac gagccgcacc atgaagtccg
     8401 cggcgaagcc ggccgccaag cggaccaccg ccaaggcgac cacccgctcc atggccaagc
     8461 cggccgccaa gcggaccacg agcagccgca ccaccagccg caccgtcaag tcggctgcca
     8521 agcccgcggc gaagcgcacc accagccgca ccacggccaa gtccatgagc aagtccatgg
     8581 cgaagccggc cgcgaagcgc accaccgcca aggcggccac ccgctccatg gccaagccgg
     8641 ccgccaagcg gaccgtcaag gtcagcagcc gctcgaccgc caagccggtg gcgaagtcgt
     8701 cggcgaagcc gaccgcgaag cgcatggtca agcggaccac cgccaaggcg gccgcccggc
     8761 cggccatgcg gaagacggcc atggcggccc gcaagaccgc ccggccgacc gccaagcgca
     8821 cgaccaggcg caccggcacg cggaagctcg cgcgggtcgc ctgacgcagc cgcaccgcat
     8881 cgaacgggcc gctccaaaag gggcggcccg ttcggcgttt ccgcaggtcc acgaaccgaa
     8941 tttacctgcg ggttgtttgc ttctccgagc agcggttagc cgactcccga ccaagaaaag
     9001 tgtcctggga aggggtaacc gggtgcgtgt gctcggcgtc aacggcgtct tccacgaccc
     9061 ggcggccgcg ctggttgtcg acggccggat cgtggcggct gccgaggagg agcgcttcag
     9121 ccgccgcaag cacggcaagc ccgcggtgcc cttctccacc tgggagctgc cggagcgggc
     9181 ggctgcctgg tgcctggcgg aggcggggct cgcacccgag gatctggacg cggtcgcgta
     9241 ttcgtatgat cccgggttgg cgccgtcggg cggtggggat gtgaccgctg acggctggga
     9301 gggcctgcgg acgctgtacg cgcggcgggc gccgttgttt ctgcgcagtg tgctgcctgg
     9361 gctcgacccg gcgtgtatcc gtttcgtacc gcaccatgtt gcccatgccg cctcggcgta
     9421 tctggcggca ccgtggccgg actccagtgt gctggtgctc gacggccgcg gcgagcgggc
     9481 ttcgcatctg gccggacggg cagccggtgg gagcctggag atcctcaggg cgcaggcgct
     9541 gccccactcg ctcgggattc tctatgagga ggtcaccgcc cacctcggct tcacgcgcgc
     9601 ctccgacgag tacaaggtga tggcgctcgc cgcctacggg aagcctgcct tcctgcggga
     9661 gttccgcgag gcggtgcggg cggacggcga cggcggcttc cgggtcgcgc cgatcgagct
     9721 ggacaccctg gcgaagcggc gggccagcga ggctgaggag ctcgcggagg agcatgccga
     9781 tctggctgcc agtctccaga cccggctgga ggaggtcctg ctggagctgg ccggctggct
     9841 gcaccgccag accggcgatt ccaccttgac gatggccggc ggcgtcgccc tcaactgtgt
     9901 cgccaactcg cgcctggccg ctgagggccc gttcgagcgg atctgggtgc agccggcggc
     9961 tggcgacgcg ggcaccgcgc tcggtgcggc gttgtgggtc gcggcggccg gcggggacca
    10021 ggtggagccg atgcggacgg ctgcccttgg ccccggctgg ggcgacgacg cgctggaggc
    10081 ctggctggtc cgcgcgaacc tgccgtatga gcggccggct gatctggccg acgcggtcgc
    10141 tcgggcgctc gccgccgatg cggtggtggc gtggttccag ggacgcagcg agtacgggcc
    10201 acgtgcgctt gggcaccgca gccttctggc gaacccggcg catgccgcga acctggagcg
    10261 cctcaacgat gtgaagggcc gcgagcagtt ccggccggtc gccccgatgg tgctggccga
    10321 gcgtgccccg gcgatcttcg agggtcccct gccgagcccg tacatgctct tcacccaccg
    10381 ggtgcggccc gcgtggcgtg agcggatccc ggcggtggtg catgtcgacg gcagcgctcg
    10441 catccagaca gtcgaccggg cggaggagcc gctggtcgcc cggctgcttg acgcggtcga
    10501 gcgccgtacc ggtgtgccgg tggcggtcaa taccagcctg aacaccgccg gccggccgat
    10561 ggtggacgac ccgcgcgacg cgctggagtg cttcggttcg gccccggtgg acgtgctcgc
    10621 gctgggaccg ttcctggtgc gccgtgcgaa cttcgcgagg ccgtgacgat gaccggcttc
    10681 gacctggtcg tccccacgct gggccggccg tcgctgcggg cgctgctcgg cgcgctcggt
    10741 tcggccgcgg ccgcgggtgg tccgctgccc ggccggctgt tgctggtcga tgaccggcgg
    10801 cagccggagg cgccgttgcc gcttgccgag ct
//
LOCUS       DASWDC010000291         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1499052, whole genome shotgun sequence.
ACCESSION   DASWDC010000291 DASWDC010000000
VERSION     DASWDC010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499052"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1114)
                     /locus_tag="VF995_09390"
     CDS             complement(17..1114)
                     /locus_tag="VF995_09390"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9343812.1"
                     /translation="MMRDKIALEEHYLSEGQLDEGHRAIDANIVKRLLDTEDLRLEAM
                     DRNGIATSVLSHSITGVQAEPDPGRAAALARELNDGLAELVARHPSRFAAFAAIPMHD
                     GDSAAAELRRAVADLGFLGAHLNGFQNVSGSGFTVTEALYYDDPRFEPFWAAAVDLSV
                     PVYLHPRFAPTDQQRIYEGYRAMASAAWGFGVETGGHALRLIVSGVFERHPQLTMILG
                     HLGEMLPYAIGRFDQCWARDARGPQSEAISQPPSHYFHRNFHVTTSGFFHTRALEAAI
                     AELGVDRVMFSVDYPYENPDAGAAWFDALPLDPADRARISARATPPASCASPEPHRGR
                     PSGWVLVPATLARSQQTPRRLPRELELRSPR"
     gene            complement(1111..2088)
                     /locus_tag="VF995_09395"
     CDS             complement(1111..2088)
                     /locus_tag="VF995_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015655658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9343813.1"
                     /translation="MRIAAFEHDGVERLGLVTDDGGGVRPLPVGVTLLELLAGTGAGT
                     QVEGVAPDAGAAVPLGEVRLLPPLRPASLRDFVSFERHVEGVTKAVEGRASVPERWHE
                     APTFLFMNPHSAIGSGAPVPIPPGAKLMDYELEVGGVLGRDGRDLTPEQARDHIAGYL
                     IVNDWSARDLQAWEMRMGLGPAKGKDFATTLGPWLVTPDELEPWRRGDRLDLAMRVAV
                     NGVERGADRLSNMAWSFEELVAYAARGAWVRAGDVIASGTCSGGALAELWGRNGRQDP
                     PPLRAGDVVTMTVEGLGTISNQLVEGPPEAAPIPVARRLRKISEPEKGA"
     gene            complement(2101..2394)
                     /locus_tag="VF995_09400"
     CDS             complement(2101..2394)
                     /locus_tag="VF995_09400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX9343814.1"
                     /translation="MPYVVLATWTAQQGSEAVVLEALTELAPISRQESACRFYQPYQD
                     PAEPRVFHIFELYDDEDGYKAHAESEHFKRLGFGKAIPALEHRERAFYQTLDV"
     gene            complement(2415..2945)
                     /locus_tag="VF995_09405"
     CDS             complement(2415..2945)
                     /locus_tag="VF995_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9343815.1"
                     /translation="MTFSERRVSADGFDLRVLEAGWGPPLVYLHGAGGLHLTSAHDLL
                     AGHHRVVAFELPGFGASPQNSRTASFDELAATMRLAVAAAGIDGGYTLTGTSFGGATA
                     LHMALAEAEAPPRPEFWGGPATVLRQLRESRQAIGWEILDGLFTMPHLPYAKAHRSLE
                     LFVKEVVPGLRAAGTA"
     gene            3081..>3346
                     /locus_tag="VF995_09410"
     CDS             3081..>3346
                     /locus_tag="VF995_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343816.1"
                     /translation="MALAGDLNLLRPVFDPGSVRRTFTVVLSDYALTVLGEPLLGIVG
                     ASAPCIRIDFRAIPDDLDLDGMDRLLLRRDLVIGPVGHGITGNG"
BASE COUNT          460 a         1121 c         1277 g          488 t
ORIGIN      
        1 ttgtcgccgg tcgccatcac cgtggacttc gcagctccag ctcccttggg agccgcctgg
       61 gagtttgctg ggaacgtgcc agcgttgccg ggacgagcac ccaaccactt gggcgccccc
      121 ggtggggttc aggggaggcg caggaggcgg gcggcgttgc cctggccgat atcctggcgc
      181 ggtcggcggg atcgaggggg agggcgtcga accaggcggc gccggcgtcg gggttttcgt
      241 aggggtagtc gaccgagaac atcacgcgat cgacgcccag ctcggcgatg gcggcttcca
      301 gggcgcgggt gtggaagaag cccgaagtcg tcacgtggaa gttgcggtgg aagtagtgcg
      361 agggcggctg cgagatcgcc tcagactgcg ggccgcgggc gtcgcgggcc cagcactggt
      421 cgaagcgccc gatcgcgtac gggagcatct cgccgaggtg gccgaggatc atggtcagct
      481 gcgggtggcg ctcgaacacg ccgctgacga tcaggcggag ggcgtggcct ccggtctcga
      541 cgccgaagcc ccaggcggcg ctggccatcg cccggtagcc ctcgtaaatc cgctgctggt
      601 ccgtcggggc gaacctgggg tggaggtaga ccgggaccga gaggtcgacg gccgccgccc
      661 agaacggctc gaagcgcggg tcgtcatagt acagcgcctc ggtgacggtg aagccgctgc
      721 cgctgacgtt ctggaaaccg ttcaggtggg cgccgaggaa gccgaggtcg gccacggcac
      781 ggcgcagctc ggcggcggcg ctgtcaccgt cgtgcatcgg gatcgctgcg aaggcggcga
      841 agcggctggg gtggcgggcg accagctcgg cgaggccgtc gttcagctcc cgggcgaggg
      901 cggcggcgcg gccggggtcc ggctcggcct ggacgcccgt gatcgagtgc gagaggacac
      961 tggtggcgat gccgttgcgg tccatcgcct ccaggcgcag gtcctcggtg tcgaggaggc
     1021 gcttgacgat gttggcgtcg atggcgcggt ggccctcgtc gagctgcccc tcggacaggt
     1081 agtgctcctc cagggcgatc ttgtcccgca tcatgctccc ttctcaggct cggagatctt
     1141 ccgcaggcgg cgggccaccg ggatgggcgc ggcctcgggt ggcccctcga ccagctggtt
     1201 gctgatcgtg cccaggccct cgacggtcat cgtcaccacg tcgccggccc gcagcggcgg
     1261 cgggtcctgg cggccgttgc ggccccacag ctcggccagc gcgccgccgg aacaggtacc
     1321 cgaggcgatc acgtccccgg cgcggaccca ggcgccgcgg gcggcgtagg cgaccagttc
     1381 ctcgaacgac caggccatgt tggagaggcg gtcggcgccc cgttcaacgc cgttgaccgc
     1441 gacgcgcatg gcgaggtcga ggcggtcgcc acggcgccag ggctccagct cgtcgggggt
     1501 gaccagccag gggccgaggg tggtggcgaa gtccttgccc ttggcggggc cgaggcccat
     1561 gcgcatctcc caggcctgga ggtccctggc ggaccagtcg ttgacgatca ggtagccggc
     1621 gatgtggtcg cgggcctgtt cgggggtcag gtcgcggccg tcgcggccga gcacgccgcc
     1681 gacttccagc tcgtagtcca tcagcttggc cccgggcggg atcggcacgg gcgccccgct
     1741 gccgatcgcc gagtgggggt tcatgaacag gaaggtcggt gcctcgtgcc agcgctcggg
     1801 gaccgacgcc ctgccctcga ccgccttggt cacgccctcg acgtggcgct cgaaggagac
     1861 gaagtcgcgc aggctcgcgg gacgcagcgg cgggagcagg cgcacctcgc cgagcgggac
     1921 ggccgctccg gcgtccggcg ccacgccctc cacctgggtg ccggcaccgg tcccggccag
     1981 caactccagg agggtgaccc cgaccggcag cgggcggacg ccaccgccgt catcggtgac
     2041 gaggccgagc cgctcgaccc cgtcatgctc gaacgcggcg atccgcatgc cggccgcgcc
     2101 tcagacgtcg agggtctggt agaaggcgcg ctcgcgatgc tccagcgcgg ggatcgcctt
     2161 gccgaagccg aggcgcttga agtgctcgga ctcggcgtgg gccttgtagc cgtcctcgtc
     2221 gtcgtagagc tcgaagatgt ggaagacccg cggctcggcg gggtcctggt agggctggta
     2281 gaagcggcag gcggactcct ggcgggagat cggcgccagc tcggtcagag cctccagcac
     2341 gacggcttcg gagccttgct gcgccgtcca ggtggcgagc acgacgtagg gcatggcaga
     2401 tcctttcgtg ggagttacgc ggtcccggcg gctctgagtc ccggcacgac ctccttgacg
     2461 aacagctcca gggagcggtg tgccttggcg tagggcaggt gcggcatggt gaacagcccg
     2521 tcgaggatct cccagccgat cgcctgccgc gactcgcgca gctgccgcag gaccgtggcc
     2581 ggtcctcccc agaactccgg gcgcggcggc gcctccgcct cagcgagtgc catgtgcaat
     2641 gcggtcgccc cgccgaagga tgtcccagtc agggtgtagc ctccgtcgat cccggccgcc
     2701 gccaccgcca ggcgcatggt cgctgccagt tcgtcgaagc tcgcggtccg cgagttctgg
     2761 ggtgatgcgc cgaacccggg cagctcgaag gcgaccaccc ggtggtgccc agccagcagg
     2821 tcgtgggcgc tggtcaggtg aaggccgccg gcgccgtgga ggtagacgag gggcgggccc
     2881 caccccgcct cgagcacgcg gaggtcgaag ccgtccgcgc tgacgcgacg ctcactgaag
     2941 gtcactcggg ctcctcggcg gggcagggaa ggggtggcgg gtgggacatc aggcggtcgt
     3001 tgggcacctg ccgcgcgtga catcgtaaaa tatcacccac tcttgtgcag tacgtcgccg
     3061 ctggcgaccg ggaggcactg atggccctgg caggcgatct gaatctcctg cgcccggtct
     3121 tcgacccggg atcggtgcgg cgcaccttca cggtggtcct gtccgactac gcgctcaccg
     3181 tccttggcga gccgctgctc gggatcgtcg gcgccagcgc gccgtgcatc cgcatcgact
     3241 tccgggcgat ccccgacgac ctggacctcg acggcatgga ccggctgctg ctgcgccgcg
     3301 acctggtgat cgggccggtc gggcacggga tcaccggcaa cggcga
//
LOCUS       DASWDC010000292        11511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1333043, whole genome shotgun sequence.
ACCESSION   DASWDC010000292 DASWDC010000000
VERSION     DASWDC010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333043"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VF995_09415"
     CDS             <1..465
                     /locus_tag="VF995_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX9343817.1"
                     /translation="DLRLEDIDLVVPHQANQRIIEATARRLKTPMSNVVTNVARYGNT
                     SAASIPIAMEEAWEQGQIRPGQHLLLTAFGAGLAWGSLVLDWTLQGPPAKQPEEPTQS
                     ALPIVHGLIDGSLWEGVPIGPPQPAGPVSGALLPDAEAATAEAATVVEAPGD"
     gene            458..1219
                     /gene="fabG"
                     /locus_tag="VF995_09420"
     CDS             458..1219
                     /gene="fabG"
                     /locus_tag="VF995_09420"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009142066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX9343818.1"
                     /translation="MTDAGKLELEGRTALVTGASGAIGRACAVALAGVGARVVVAYSS
                     DEQGARDTAELVKAAGAETVVAQADLADADGAKRLVEAAGSGGVDVLVNNAGVTRDGL
                     VLRMRDEDFARVLDVNLVAAFRCTREALRGMLRRRWGRVISISSVVGLIGNPGQSNYA
                     AAKAGLIGFSMSVAREVASRGITVNVVAPGYIPSKLTEAMSEEAKQATFSAIPAGRMG
                     TPEEVAAAVRFLAGEQAAYITGQVLAVDGGVTMGA"
     gene            1289..1546
                     /gene="acpP"
                     /locus_tag="VF995_09425"
     CDS             1289..1546
                     /gene="acpP"
                     /locus_tag="VF995_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9343819.1"
                     /translation="MPESREEVVDTMKEVLSSELGVDADKITADARFKEDLDADSLDL
                     VEVVLALEEKFGIEIPDEEIAGVKTVGEAADLVISKQSAVG"
     gene            1552..2790
                     /gene="fabF"
                     /locus_tag="VF995_09430"
     CDS             1552..2790
                     /gene="fabF"
                     /locus_tag="VF995_09430"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX9343820.1"
                     /translation="MRRVVVTGLGAVTPVGVGVRAYWDGICAARSGVGPLTLVDPEQL
                     PSKVAAECLDFDPAAHLGAKEARRLDRSTQFALSAAREAWADAGIEGTLDRDETGVVF
                     GTGIGGITSLLASDKVLHAKGPNRVSPFTVPQLMPNAAAGQIAMAFKLRGPNFTTTTA
                     CAASNHAIGLAFRMIQSGEAQAMVAGGTESAFADIALVAFAQMTALSTHFNDRPEQAS
                     RPFDGRRDGFVMGEGAAALILEEREAALRRGARVYAELAGFGQSADAFHITAPSEDGG
                     GAALAMRRALRSSGIDPEAIGYVNAHGTSTPQGDVSETRAIRLTFGDHADRLAVSSTK
                     SMIGHLFGAAGAVEGVATILALQEGVLPPTINQTDPDPACDLDYIPNEARKADVEAAM
                     SNGFGFGGHNAVVVFRRHQA"
     gene            complement(2834..3970)
                     /locus_tag="VF995_09435"
     CDS             complement(2834..3970)
                     /locus_tag="VF995_09435"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX9343821.1"
                     /translation="MLLRVRHLLVALVAAVLAAGWSAVPAAAQAQAAPPLVGAPEGVV
                     MDVATGAVLWQRDEHRPVPIASTTKILTALVAQDSYPADQVLTVPTAAEKVDGTRFGY
                     QAGMRIKLHDLFTALLMVSANDAAETLAAAYPHGGRAGFVRAMNDEAAAYGCTDSSWR
                     DPSGLDAPGHRASAADLAVLGRALLARPLLAKIVATRSTPYRWPSGHVQILDNHNHFV
                     SFGRDPGAIGIKTGFTDLAHATIVAAQRRGGRTLIAVALGAPTAAASYGDVRAMFAYG
                     FATPASPLDEVLGRGARRGLAAGSAADVRLLPKRQAAANGSLSHVLRHRLLGAPLPLA
                     LTVVVFLVMAGAVVVVGWRPGVTYGGSHAARSRSRARAARREDA"
     gene            4657..6456
                     /locus_tag="VF995_09440"
     CDS             4657..6456
                     /locus_tag="VF995_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9343822.1"
                     /translation="MVAVVALLAVSFVIFRQSHRTSAAASCGQGQGQLPLTVVASPDA
                     AGPLRQVAQSYTRANHVVAGRCVSVSVQSLEASQAGAALAKGWTGAPAVPRPDVWVPD
                     SSSWAQLIELQLRQAHQVDPIPTERPSIATSPLVIAMPEPMAQALGWPRRPIGWGDLL
                     TALQNPQGWAAFHHPEWGAVKLGKTDPTQSTSGLEGVLGALTAGLGGPISPQALTAHS
                     EQVRELILGLERAPGEQSDTDSNFLVSLRQADDNGGALKFVSAVPLGEQQVLDYNQGN
                     PGGDPGTLGDHAKPKVPLAAVYPKEGTLEADHPWLVLHEAWVDDAKRSAASAFLNYLL
                     SPTVQAKLQAAGLRDPDGKAGSLATEANGLIASQPARVIPNPSASVVGGIIRGWSAAS
                     RRGNVLAVFDVSGSMNEPVPGTHTTKMGLVRQAAAAATGLWAPEDNVGIWAFSTKLPP
                     NGKDFQEIVPLGPVDAQIPGTNRTRRELLGAGVSKLQATTHDTALYETALGAFQLVKS
                     HFVPDRINTVVLLTDGINDDPDNALSLQQLVQQLKAGEAGGQQVRIITIGYGRDADAN
                     ALRAIAQATGGAYFSAADPRGIRSIFIQALSNF"
     gene            6541..8328
                     /locus_tag="VF995_09445"
     CDS             6541..8328
                     /locus_tag="VF995_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9343823.1"
                     /translation="MRRFLVGGLVTALAVTLAAPVTARPFIPPALPGAEMTDMAWAAG
                     SHGAPPTGGTQAYVVLYASGASAAVARAALRASGATIVAENRWVGLATVRTSNPQFLE
                     AVAGERALAGAAHDDAIGWAPPQATASASANASSDPDPDHSNGAARGTATTMAAPGTG
                     RAPGARNRTTGPEPLSARQWDMRVIGATPDGSYRVNQGNRGVLVGVIDTGVDGGHPDI
                     APNFDRDLSRNFTRDIPSVDGPCEHPSCVDPADEDDEGHGTHAAGTIAAALNGLGIGG
                     VAPGVTLVSLRAGQDSGLFFLQPVVDALTYAARNGIDVVNMSFYTDPWLYNCPDNPAD
                     TPDEQRQQRIIIAAMQRAVTFARRHGVTPIAALGNEHTDLDHPATDDTSPDYPPQSAR
                     RRSITDACVSVPTETKGVVAVSALGPDGRKAAYSNWGLGRTTLAAPGGDQDDFPGSGQ
                     YGQPENAVLSAYPENVARANHDLRRDGTPANPSVLRDCQHGVCAYYQYLEGTSMAAPH
                     VAGVAALIVSALGRPDPRHGGLTMDPDRVIRILRRSATKTACPAPVLTYPGHGSDYDA
                     PCQGSRHDNSVYGHGIVNALNAVLPQALQ"
     gene            8338..8429
                     /locus_tag="VF995_09450"
     tRNA            8338..8429
                     /locus_tag="VF995_09450"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8372..8374,aa:Sec,seq:tca)
     gene            complement(8618..9208)
                     /locus_tag="VF995_09455"
     CDS             complement(8618..9208)
                     /locus_tag="VF995_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEX9343824.1"
                     /translation="MIEAELKAHLREPERIRQALRALPAEGEEMCVYHDTYYDWPDGR
                     LVGGDRELRVRRIDIIDGDRRRRCLLTYKDAAADPASGSKPEWETAVEEPEAIDAILR
                     GLGFEHDIAFEKHCTNYTFHAQGRRLQATLARVPELDGSFLEVETLVEDNAELQPALD
                     AIHRVLDDLRIPRSDLTNELYTDLVRRQRAAARQWP"
     gene            complement(9263..9742)
                     /locus_tag="VF995_09460"
     CDS             complement(9263..9742)
                     /locus_tag="VF995_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5919 domain-containing protein"
                     /protein_id="HEX9343825.1"
                     /translation="MQGLDDDEDLAAVLAVRGLGILGLNDSLLRAALTGRLREGRLTV
                     RALLLNPDSLAARRRAAEIGESVESFASGIQLSVARLRELVGTHPAVSLEVYEYDTLP
                     VWRLVDIDGTVYVAVFAEDWEGHESPVYKVTATPRGVLHRGFRRMFEDLRQGAKRII"
     gene            complement(10240..10683)
                     /locus_tag="VF995_09465"
     CDS             complement(10240..10683)
                     /locus_tag="VF995_09465"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9343826.1"
                     /translation="MGKHGDEGQGAAPRWLIDGMNLIGSRPNRWWNDPDAAVRRLIGE
                     LDRYAAASGEDVIVMFDRRPPDVMAGTHGAVQVAFASRRGRDAADHEIVQLVAADPVP
                     GSFRVVTSDRALVERVRDLGAQTSPASSFRRQLDQVLAATPEPGS"
     gene            10748..>11511
                     /locus_tag="VF995_09470"
     CDS             10748..>11511
                     /locus_tag="VF995_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9343827.1"
                     /translation="MGTADADWDWELVVVGAGPAGAAAAIEARRIDPEARVLLLDRAV
                     FPRDKACGDGIGPHAVAELVSLGAGAALDGYPPIRRLRVRAPGGIEIAGEPAEPSWVV
                     PRRVLDARLVDLAIAAGVEFRQERVRSLRQRDGAVVVNGTLTGRVVVGADGANSVVRR
                     LLGVPSSADRHTAIALRGYAPAPTGSHNGDGVAAAEPEQLIGWVADGWPAYVWSFPTG
                     TGLVNAGYGLLRSRLEGGRAELERRLRVLLPGVDPDP"
BASE COUNT         1725 a         4114 c         4154 g         1518 t
ORIGIN      
        1 gacctgcggc tggaggacat cgacctggtg gtcccccacc aggccaacca gcggatcatc
       61 gaggcgaccg cccggcggct caagacgccg atgagcaatg tcgtcaccaa cgtcgcccgc
      121 tacggcaaca cctcggcggc ctccatcccg atcgccatgg aggaggcctg ggagcagggc
      181 cagatccgcc ccgggcagca cctgctgctg accgccttcg gcgccggcct ggcgtggggc
      241 tcgctggtgc tcgactggac gctccagggc ccgccggcga agcagccgga ggagcccacg
      301 cagtcggccc tgccgattgt gcacggcctg atcgacgggt cgctgtggga gggcgtgccg
      361 atcgggccgc cacaacccgc gggcccggtg agcggcgccc tcctcccgga cgccgaggca
      421 gcgacggcgg aggcggcgac ggtcgtggag gcgcctggtg actgacgccg gcaagcttga
      481 gctggaaggg cggaccgccc tggtcacggg cgcgtcgggg gcgatcggac gggcctgcgc
      541 ggtcgccctc gccggcgtcg gggcacgggt ggtggtcgcc tactcgagcg acgagcaggg
      601 cgcgcgggac accgccgagc tggtcaaggc cgcgggggcc gagacggtgg tggcacaggc
      661 ggacctggcc gacgcggacg gagccaagcg gctggtcgag gcggccgggt ctggcggggt
      721 cgacgtcctg gtcaacaacg ccggcgtgac ccgcgacggg ctggtgctgc ggatgcgcga
      781 cgaggatttc gcgcgggtgc tggacgtcaa cctggtggcc gcgttccgtt gtacgcgcga
      841 ggcgcttcgt gggatgctgc gacgccgctg gggccgggtg atctccatct ccagcgtcgt
      901 cgggctgatc ggcaatcccg gccagtccaa ctacgccgcc gccaaggcgg gcctcatcgg
      961 cttctccatg agcgtggccc gcgaggtcgc cagtcggggc atcacggtca acgtggtcgc
     1021 gcccggctac atcccgtcca aactgactga ggcaatgagt gaggaggcca agcaggcgac
     1081 gttcagcgcg atccccgccg ggcggatggg gacgcccgag gaggtggccg ccgccgtacg
     1141 gttcctcgcc ggcgagcagg ccgcctacat caccggtcag gtcctggcag tcgacggtgg
     1201 cgtcaccatg ggagcctgag cagcaaggca acgaaacaga gccaacgaag caccgcaaca
     1261 gaggcggacg agaaggagag catgcactat gccggagtcg cgcgaagagg tcgtcgacac
     1321 catgaaggag gtcctctcct ccgagctcgg cgtcgacgcc gacaagatca ccgctgacgc
     1381 ccgcttcaag gaggacctcg acgcggactc cctcgacctg gtcgaggtgg tcctggcgct
     1441 cgaggagaag ttcggcatcg agatcccgga cgaggagatc gccggcgtca aaaccgtcgg
     1501 agaggcggcc gacctggtga tctccaagca gagcgcggtg gggtagcgaa cgtgcgacgc
     1561 gtcgtcgtca ccggcctcgg tgcggtgacc ccggtcggtg tcggcgtccg ggcgtactgg
     1621 gacgggatct gtgccgctcg ctcgggggtc ggcccgctca ccctggtcga ccccgagcag
     1681 ctcccgtcca aggtcgccgc cgagtgcctc gacttcgatc cggcggcgca cctcggggcc
     1741 aaggaggcac gccgcctcga ccgctcgacc cagttcgctc tcagtgcagc ccgcgaggcg
     1801 tgggccgacg ccgggatcga ggggacgctc gaccgggacg agaccggcgt ggtgttcggc
     1861 accggcatcg gcgggatcac ctcgctgctc gccagcgaca aggtgctgca cgccaagggc
     1921 ccgaaccggg tgtcgccatt caccgtgccg cagctcatgc ccaacgcggc cgccgggcag
     1981 atcgctatgg cgttcaagct gcgcggcccg aacttcacca ccacgaccgc gtgcgccgcc
     2041 tccaaccacg ccatcggtct cgccttccgg atgatccaga gcggcgaggc gcaggcgatg
     2101 gtggccggcg gcaccgagtc cgccttcgcc gatatcgcct tggtggcgtt cgcgcagatg
     2161 accgccctgt cgacccattt caacgaccgt cccgagcagg cctcacgccc gttcgacggg
     2221 cggcgcgacg gcttcgtcat gggcgagggc gcggccgcgc tgatcctgga ggagcgcgag
     2281 gcggcgctgc ggcggggcgc gcgcgtctat gccgagctgg ccggcttcgg gcagtcggcc
     2341 gacgccttcc acatcaccgc gccctctgag gacggcggcg gcgccgctct ggcgatgcgc
     2401 agggcgctgc gctccagcgg catcgacccg gaggcgatcg gctatgtcaa cgcccacggc
     2461 acctccaccc cgcaggggga cgtctcggag acgagggcga tccggctgac gttcggcgac
     2521 cacgccgacc ggctggcggt ctcgtcgacc aagtcgatga tcggccacct gttcggcgcg
     2581 gcgggcgcgg tcgagggggt cgccaccatc ctcgcgctgc aggagggcgt gctgcccccg
     2641 accatcaacc agaccgaccc ggatcccgcc tgcgacctgg actacatccc caacgaggcc
     2701 cgcaaggccg acgtcgaggc ggccatgtcg aacgggttcg ggttcggcgg ccacaacgcg
     2761 gtggtggtgt tccgccgcca ccaggcgtag ccacgaggcg tagccaccag gcgtaggcgc
     2821 gggcaggttc ggctcaggcg tcctcgcggc gggcggcccg tgcgcgcgag cgcgagcggg
     2881 ccgcgtggct gccaccgtag gtgacgcccg gccgccagcc gacgaccaca acggcgccgg
     2941 ccatgaccag gaacaccacc accgtgaggg cgagcgggag cggtgctccc agcagccggt
     3001 gccgcagcac gtgggagagg gaaccgttgg cggctgcctg ccgtttgggg agcagccgga
     3061 cgtcggcggc gctgccagcg gccaggcccc ggcgggcgcc gcggccgagc acctcgtcca
     3121 gcgggctcgc cggagtggcg aagccgtagg cgaacatcgc gcggacgtcg ccatagctgg
     3181 cggcggctgt cggtgcgccg agcgccaccg cgatcagggt gcggccgcct cggcgctgcg
     3241 cggccacgat ggtggcgtgg gcgaggtcgg tgaagccggt cttgatcccg attgctcccg
     3301 ggtcgcgccc gaagctgacg aaatggttgt ggttgtcgag gatctgcacg tgcccgctcg
     3361 gccagcggta gggcgtcgag cgggtggcga cgatcttggc cagcagcggc ctggcgagca
     3421 gcgcccgccc gaggaccgcg aggtcggccg cgctggcccg gtggccgggg gcgtccaggc
     3481 cggaggggtc gcgccaggag gagtcggtgc agccgtaggc ggcggcctcg tcgttcatgg
     3541 cgcggacgaa acccgccctg cctccatggg ggtaggcggc ggcgagcgtc tcggccgcgt
     3601 cgttggccga gaccatcagc agcgccgtga acaggtcgtg cagtttgatg cgcatgcccg
     3661 cctggtagcc gaagcgggtg ccgtccacct tctcggccgc cgtggggacg gtgagcacct
     3721 ggtcggccgg gtagctgtcc tgtgccacca gggcggtcag gatcttggtg gtggaggcga
     3781 tcggcacggg gcgatgctcg tcgcgctgcc acagcaccgc gccggtggcc acgtccatca
     3841 ccacgccctc cggcgcaccg accagcggtg gggccgcctg ggcctgcgcg gcggcgggaa
     3901 cggcgctcca gccggcggcc aggacggcgg cgaccagcgc gacgagcagg tgacggactc
     3961 ggaggagcac tggggaagcg tgcatggcgc ccagtatacg gcgcaagcca gccgagcgtg
     4021 acaatcaagg cagagggcaa gggaccacgc ggtcacgagg accgtcaggg ggccggtcgc
     4081 ctgggccaac tggagccaag cgaccggcga cacccgtcct tcccgcccga gacggcgcga
     4141 cgcggaaccg cccgccgtcc ggggagtctc gcccagaggc gcggcaccag cccttcgact
     4201 ggcgggcacc ccctgctgcg tcggcccggg gatccggccc ttcgaccgga ccgccggatc
     4261 gaccggcgat actccaagag tgacacaaca tcgccgactt tgcccacccc agtggatgaa
     4321 gtgagcatgg cggtcatccg aggagcagca gtcggctccc ctggaaccga tttgatccct
     4381 ctaaatagaa aagaaacctg aaagcatcga tcctggcaag gacttttacc tccagaacgt
     4441 tccaattagg gcgccgtttt gcattgactc gcggaacgcg gcattctcta cgctcgcccc
     4501 cataatgcac cggcctgtcc ccggtgtgtc tacgtgattt cctatttcga tcagggagcc
     4561 caagcgaggc ggaagtagcg aatgtcacag acttcttcgg gaaggcattt ggcgacacgg
     4621 ccgggccgcc cctcgggcct tgccacctcg atgatcgtgg tggcggtcgt ggccctgctg
     4681 gccgtcagct tcgtgatctt caggcagtcg catcgcacca gtgccgccgc cagctgcggc
     4741 cagggccagg gccagcttcc gctgacggtg gtcgcctccc cggacgctgc cggcccgctc
     4801 cgccaggtgg cccagagcta cactcgggcc aaccacgtgg tcgccggccg ctgcgtcagc
     4861 gtgtcggtcc agagcctgga ggcgtcccag gccggcgcgg ccctggccaa gggctggacc
     4921 ggcgcgcccg cggtgccgcg cccggacgtc tgggtgcccg actcctccag ctgggcgcag
     4981 ctgatcgaac tccagctgcg gcaggctcac caggtcgacc cgatccccac cgagcgaccg
     5041 agcatcgcca cctcgccgct ggtgatcgcc atgccggagc cgatggcgca ggcgctgggc
     5101 tggccgcggc ggccgatcgg ctggggcgac ctgctcaccg cgctccagaa cccgcagggt
     5161 tgggcggcgt tccaccatcc cgaatggggg gccgtcaagc tcggcaagac cgaccccacc
     5221 cagtcgacct ccgggctgga gggcgtgctc ggcgcgctga ccgctgggct cggcggcccg
     5281 atctccccgc aggcgctgac cgcccacagc gagcaggtgc gcgaactgat cctcgggctg
     5341 gagcgggcgc ccggcgagca gtccgacacc gacagcaact tcctggtcag cctgcggcag
     5401 gccgacgaca atggcggggc gctcaagttc gtctccgcgg tcccgctggg cgagcagcag
     5461 gtgctcgact acaaccaggg caatcccggc ggcgacccgg gcaccctggg cgaccacgcc
     5521 aagccgaagg tccccttggc cgcggtctac ccgaaggagg gcacccttga ggccgaccat
     5581 ccctggctgg tgctgcacga ggcgtgggtg gacgacgcca agcggagcgc ggcctcggcg
     5641 ttcctgaact acctgctctc ccccacggtt caggcgaagc tccaggccgc cgggctgcgc
     5701 gaccccgacg gcaaggccgg ctcgctggcg accgaggcca acggcctgat cgccagtcag
     5761 ccggcgcggg tcatccccaa ccccagcgcg tcggtggtcg gggggatcat caggggctgg
     5821 agcgccgcct ccaggcgcgg caacgtgctg gccgtcttcg acgtctccgg ctcgatgaac
     5881 gagccggtcc ccggcaccca cacgaccaag atgggcctgg tcaggcaggc cgccgccgcc
     5941 gcgaccggcc tgtgggcgcc ggaggacaac gtcggcatct gggcgttctc caccaagctg
     6001 ccccccaacg gcaaggattt ccaggagatc gtgccgctcg gcccggtcga cgcgcagatc
     6061 cccggcacca accggacccg ccgcgagctg ctcggcgccg gcgtctcgaa gctgcaggcc
     6121 accacgcacg acaccgccct ctacgaaacg gctctgggtg ccttccagct cgtgaagagc
     6181 catttcgtgc ccgaccggat caatacggtg gtgctgctga ccgatggcat caacgacgac
     6241 cccgacaacg ccctcagtct ccagcagctc gtccagcagc tcaaggccgg cgaggcgggc
     6301 ggccagcagg tccgcatcat caccatcggc tacgggcgcg acgccgacgc gaacgccctg
     6361 cgggcgatcg cccaggcgac gggcggcgcc tacttctccg ccgccgatcc ccgcgggatc
     6421 cggagcatct ttattcaggc cctgtcgaac ttctgacgac ctagagagag tcgttcgcgg
     6481 tttcgtcgag gtgccggacc cgccgccggc ctgcgtgcct tcgggcgtgg gggtggggcg
     6541 atgcggaggt tcctcgttgg cggcctggtc acggcactgg cggtgacgct cgctgcgccg
     6601 gtgaccgcga gaccgttcat cccgccggcg cttcccggcg ccgaaatgac cgatatggcc
     6661 tgggcagccg gctcccacgg cgcgccgccg acgggcggga cgcaggccta cgtggtgctc
     6721 tacgccagcg gcgcgtcagc ggccgtggcc cgcgcggcgc tcagggcgtc cggcgccacc
     6781 atcgtggcgg agaaccgctg ggtggggctg gccacggtgc ggaccagcaa tccccagttc
     6841 ctggaggcgg tggccggaga gcgggccctg gccggtgccg cgcacgacga cgccatcggc
     6901 tgggccccgc cccaggcgac cgcgagcgcg agcgcgaacg cgagctcgga cccggacccg
     6961 gaccactcga acggcgcagc gcgcggcacc gcgaccacga tggcggcgcc gggcaccggc
     7021 agggcgccgg gcgcccgcaa ccgcaccacc ggcccggagc cgctcagcgc acggcagtgg
     7081 gacatgcgcg tgatcggcgc cacaccggat ggctcctacc gggtcaacca gggcaaccgg
     7141 ggcgtcctcg tcggcgtgat cgacaccggc gtcgacggcg gccaccccga catcgccccc
     7201 aacttcgacc gcgacctcag ccgcaacttc acccgcgaca tcccgtcggt cgacgggccc
     7261 tgcgagcacc cctcctgcgt cgacccggcc gacgaggacg acgagggcca cggcacccac
     7321 gcggcgggga ccatcgctgc cgccctgaac ggcctcggga tcggcggggt cgcaccgggc
     7381 gtcaccctgg tgagcctgcg cgccgggcag gactccggcc tgttcttcct gcagccggtg
     7441 gtggacgcgc tgacctacgc ggcccgcaac ggcatcgacg tggtcaacat gagcttctac
     7501 accgacccgt ggctctacaa ctgcccggac aaccccgctg acaccccgga cgagcagcgg
     7561 cagcagcgga tcatcatcgc ggcgatgcag cgggcggtga ccttcgcccg ccgccacggc
     7621 gtcaccccga tcgctgccct cggcaatgag cacaccgacc tcgaccatcc ggccaccgac
     7681 gacaccagcc ccgactaccc gccgcagtcc gccaggcgcc gcagcatcac cgacgcctgc
     7741 gtgagcgtcc caaccgagac caagggggtc gtcgccgtct cagcgctggg ccccgacgga
     7801 cgcaaggccg cctactccaa ctggggcctc ggccgcacca ccctcgccgc acccggcggc
     7861 gaccaggacg atttcccagg gtccggccag tacggccagc cggagaacgc cgtcctgtcc
     7921 gcgtacccgg aaaacgtcgc cagggccaac cacgacctgc gccgggacgg cacccccgcc
     7981 aacccgtcgg tgctgcggga ctgccagcac ggcgtgtgcg cctactacca gtacctggag
     8041 ggaacctcga tggccgcccc gcacgtcgcc ggggtggccg ccctgatcgt cagcgccctc
     8101 ggccgcccgg acccgcgcca cggcggcctc accatggacc cggaccgggt catccggatc
     8161 ctgcgccgca gcgccaccaa gaccgcctgc cccgcgccag tcctcaccta ccccgggcac
     8221 gggtccgact acgacgcccc ctgccagggc agccgccacg acaacagcgt ctacggccat
     8281 ggcatcgtca acgccctcaa cgccgtgctg ccacaagccc tccagtaaga tcgccgtgga
     8341 ggtgtccggg tcctggtggg ccccgcggtc ttcaaaaccg ttgaggcggg cgtcccccgt
     8401 ctggcgggtt cgattcccgt ccgcctccgc tagctgggaa aatgcaagag agcgccgcct
     8461 tggcggcgac cgctgacgcg ctggagcggg ccatcgctgc cgccaaggtc gaggccgagc
     8521 aggaccaata gcgtgttact gacccaacgc ggtcgccgag ttcagcgcgt gcaggaggcc
     8581 ctggcggcga ccaagcgggg ccctcgcagc atcggcctca aggccattgg cgagccgctg
     8641 cgcgctgccg ccgaacaagg tcggtataca gctcgttggt caggtcgctt cgggggatgc
     8701 gcaggtcgtc gaggacgcga tggatggcgt cgagcgccgg ctgaagttct gcattgtcct
     8761 cgacgagcgt ctcgacctcc aagaaggacc cgtccagctc ggggacgcgg gcgagcgttg
     8821 cttggaggcg ccgaccctgc gcgtggaagg tgtagttggt gcagtgcttc tcgaaggcga
     8881 tgtcatgctc gaagccgagg ccgcggagga tcgcgtcgat cgcctcgggc tcctcgaccg
     8941 cggtctccca ttcgggtttc gacccactcg caggatcagc cgccgcgtcc ttgtaggtca
     9001 gcaagcaccg gcgccgccga tccccgtcga tgatgtcgat gcggcggacg cgaagctctc
     9061 ggtcgcctcc gacaagcctg ccatctggcc agtcgtagta ggtgtcgtgg tacacgcaca
     9121 tctcctcgcc ctcggctggc agcgcacgga gcgcctgccg aattcgctcg ggctcccgca
     9181 ggtgggcttt gagctcggcc tcgatcatgg tgcctccgac ggtgacctca tcacatcaca
     9241 tcgctccaca tcgcttgtcg ggtcagatga tgcgcttggc gccctggcgg agatcctcga
     9301 acatgcggcg aaacccgcgg tgcagcacgc cgcgtggcgt ggccgtgacc ttgtagaccg
     9361 gcgactcgtg gccttcccag tcctccgcga acaccgcgac gtacaccgtg ccgtcgatat
     9421 ccaccagccg ccagaccggc agggtgtcgt actcgtagac ctccagcgag acggccgggt
     9481 gggtgcctac cagctcgcgg agcctcgcaa ccgaaagctg gatgccgctg gcgaacgact
     9541 cgaccgactc gccgatctcc gccgcgcggc gccgcgcggc cagcgaatcc gggttaagaa
     9601 gcagcgcccg gaccgtcagg cggccctccc gcagccggcc ggtgagcgcg gcgcgcagca
     9661 gcgagtcatt caacccgagg atgccgaggc cacggacggc cagaaccgcc gccaggtcct
     9721 cgtcatcgtc gagaccctgc acggcctgtg cgaaggcaat ggggtgaatg cggcggggat
     9781 cagatgggcg acggagcacc tcgccgaagc gctggtgacc ctcggttcga cccatccagc
     9841 cattcaactg gccgaccagc tggccagcgg cgcgcagggg agggacgagc atccgccgct
     9901 gctgacccgc ccgcacgact ggccacgagc aaggtaaagg tcgtcaagga cgccaaggcg
     9961 ccaggctggg ggagcagtca ccctccggtc ccagcctggc gggcgaggag ccgacggcga
    10021 ggtcgaggga gcgcgtacag ggagaccgct gcatgccaac agcggtgcgc ttggacttcc
    10081 ctcgccgtcg gcccgcccca accgtctcga ccctcaagcc caacagtccg actgcagcca
    10141 agcttgcagc caaacaggga gggacgaccg gggagcgggc gtccccctcc tggcgggttc
    10201 gattcccgtc cgcctccgct gagctgtcga acccgggggt cagctcccgg gttcgggcgt
    10261 cgccgccagc acctggtcca gctggcggcg gaaggagctg gcggggctgg tctgggcgcc
    10321 gaggtcgcgg acccgctcga ccagggcgcg gtcggaggtg accacgcgga aggaccctgg
    10381 gaccgggtcg gccgcgacca gctggacgat ctcgtggtcg gcggcgtcgc ggccgcggcg
    10441 ggaggcgaag gcgacctgga ccgcgccgtg cgtgccggcc atgacgtccg gagggcggcg
    10501 gtcgaacatc acgatgacgt cctcgccgct ggcggcagcg tagcggtcaa gctcgccgat
    10561 gaggcgccgg acggcggcgt ccgggtcgtt ccaccagcgg ttcgggcggg agccgatcag
    10621 gttcatgccg tcgatcagcc agcgcggcgc ggcgccctga ccctcgtccc cgtgcttccc
    10681 catttgagga gcctaccgga gggcggaccg gcatgcgcga actcctccga ggcgggagac
    10741 tggcgctgtg gggacagcgg atgcggattg ggactgggag ctggtcgtgg tcggggccgg
    10801 gccggcgggc gcggccgccg cgatcgaggc caggcggatc gaccccgagg cgcgggtgct
    10861 gctgctggac cgggcggtgt ttccccggga caaggcgtgt ggcgacggga tcgggccgca
    10921 tgccgtcgcg gagctggtct cgttgggagc gggagcggcg ctcgacggct acccgccgat
    10981 ccgccggctg cgggtgcggg cgcctggcgg gatcgaaatc gccggggagc ccgccgagcc
    11041 gagctgggtg gtgccccggc gggtgctgga cgcccggctg gtcgatctgg ccatcgccgc
    11101 cggggtggag ttccgccagg agcgggtacg gagcctgcgc cagcgcgacg gcgcggtggt
    11161 cgtcaacggg acgctgaccg gccgggtggt ggtcggggcg gacggggcca actcggtggt
    11221 gcgccgcctg ctcggcgtgc cctccagcgc tgaccggcac accgcgatcg cgctgcgcgg
    11281 ctacgcgccc gccccgaccg ggtcgcacaa cggcgacggc gtggccgcgg ccgagccgga
    11341 gcagctgatc ggctgggtcg cggacggctg gccagcctat gtctggagct tcccgaccgg
    11401 gaccggcctg gtgaacgccg gctacgggct gctgcgcagc cgcctggagg ggggcagggc
    11461 cgagcttgag cggcggctgc gtgtgctgct gcccggtgtc gatccggatc c
//
LOCUS       DASWDC010000293         5140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1499205, whole genome shotgun sequence.
ACCESSION   DASWDC010000293 DASWDC010000000
VERSION     DASWDC010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499205"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VF995_09475"
     CDS             complement(<1..354)
                     /locus_tag="VF995_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007976298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurase"
                     /protein_id="HEX9343828.1"
                     /translation="MNVEAINIGPSDAIAPVNAVTAVAGKGLKGDRYFFDDGAGPGEA
                     LTLIEAEALEAVGLTGPQARRQVVVRGVRLNDLVGKRFRVGEVECYGVELCEPCLHLE
                     ELTRPGIIRDLVHRAG"
     gene            complement(396..722)
                     /locus_tag="VF995_09480"
     CDS             complement(396..722)
                     /locus_tag="VF995_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019884482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343829.1"
                     /translation="MPAEARGRKVPALTDAECSFIDHYLAIVDLVARLNPARSRGHAY
                     ACLRAAQSLTGEARALLAALELMWERGEREIHVPTLTQALRALDGERRAGLVTLPPAP
                     AEPEGH"
     gene            complement(902..3271)
                     /locus_tag="VF995_09485"
     CDS             complement(902..3271)
                     /locus_tag="VF995_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEX9343830.1"
                     /translation="MRIAVIGGGPGGLYFAALAKQLDAAHEITVWERNAADDTFGFGV
                     VFSDETLGGIEHADAAVYARMERDFARWDDIDVHYRGQVLTSGGHGFAAMSRRRLLRL
                     LQERCQALGVSMHFQRWAPDSDELAATHDLVVACDGVNSAVRARYAASFHPAIDARAC
                     QYMWLGTERVFDAFKFYVLETPHGVMQVHGYPYDTTGSTFIVEMHESVWRAAGFDAYA
                     DQTFAPGDSDEKSIARIRELFAEVLDGHEILANNSRWTRFRTIRCQSWRHGNVVLLGD
                     AAHTAHFSIGSGTKLAMEDALALAACLHEQDTLEAALGAYEAERRPVVASTQRAAQAS
                     LEWFENLGQYTDQEPPQFAFNIMTRSRRVTYENLRIRDPEFVASVDAWFAGHLRRQGT
                     APGDPRPPMFQPFRLGELELANRVVVSAMDMYRAVDGLPNDYHLVHLGGKALGGAGLV
                     MTEMACVSDTGRITPGCSGLYSDAQAAAWRRITEFVHRESPARIGVQLGHSGRKGSTK
                     LMWEGMDDPLDEGNWEVVAPSPLPYRPGVNQLPRELTAADLDEIREQFATATQRAEAA
                     GFDLVELHCAHGYLLSSFISPLSNQRRDRYGGSLANRLRYPLEVFAAMRAVWPAHKPM
                     TVRISATDWAEGGITGADAVEVARAFKAAGAAAIDVSTGQVTPDERPDFGRSYQTPFA
                     DAIRNQVEIATIAVGAISSYDDVNSIVLAGRADLCALGRVHLYDPSWTLHAAAEQGYD
                     GPGATWPQPWRAGRRRPPTSRSDGPRPRLQLIREGAAATRHARWRPPSS"
     gene            complement(3293..4930)
                     /locus_tag="VF995_09490"
     CDS             complement(3293..4930)
                     /locus_tag="VF995_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9343831.1"
                     /translation="MQLTRSAHVDTFCRDHLPPAEEWPELRFDLPEVRYPERLNCATE
                     LLDRTAQRYGADRECLRSPGERWTYGELLGAANRVAHVLVDDCGVVPGNRVLLRGPNN
                     PWLVACWFGVLKAGAVAVTTMPLLRPTELTRIAEIAAVDLALCDHRFTGDLLGADIAG
                     LRLVTYGGEDAADLSRRVDAKPARFADADTAADDVALLAFTSGTTGRPKATMHFHRDV
                     LAIADTFSRHLLRPNPADLFTGTPPLAFTFGLGGLVVFPLRAGAATLLIEKASAGELA
                     DLIAKHAVSVCFTAPTAYRAMLGAGKAAQLTTLRTGVSAGEHLPGSTWRAFHQATGVK
                     LIDGIGTTELLHIFIATAGDEIRPGSTGRVVPGYAAAILDEAGQPLPPGQLGRLAVKG
                     PTGCRYLADPRQREYVRHGWNLTGDTYVQDEDGYFWYQARSDDMIISSGYNIAGPEVE
                     EALLAHGDVAECGVVGVPDADRGQLVTAYVVLRPGVAGDEAKIRELQEFARQRIAPYK
                     YPRAVVFVDSLPRTSTGKLQRFRLRQAGAGAAPAPAT"
BASE COUNT          676 a         1828 c         1911 g          725 t
ORIGIN      
        1 cccggcccgg tgcaccaggt ccctgatgat gcccggccgc gtcagctcct cgaggtggag
       61 acagggctcg cacagctcga cgccgtagca ctcgacctcg ccgacccgga agcgcttgcc
      121 gaccaggtcg ttcagccgca ccccgcgcac caccacctgg cgccgcgcct gggggccagt
      181 gaggccgacg gcctccagcg cctccgcctc gatcagcgtc agcgcctcgc cggggccggc
      241 gccgtcgtcg aagaagtagc ggtcaccctt gagtcccttg cccgccaccg cggtgacggc
      301 gttgaccggc gcgatcgcgt cgctcggtcc gatgttgatt gcttcgacat tcattgggcg
      361 attgtgcctg gccagcagga caaccgccag gcaattcagt gcccctccgg ctccgctggc
      421 gccggcggca gggtgacgag gccggcgcgg cgctcgccgt cgagcgcgcg cagcgcctgg
      481 gtgagggtcg ggacgtggat ctcgcgctcg ccgcgctccc acatcagctc cagcgccgcc
      541 agcagcgccc tggcctcgcc ggtgagcgac tgggcggccc gcaggcaggc gtaggcgtgg
      601 ccccggctgc gggcggggtt gaggcgcgcg accaggtcca cgatcgcgag gtagtggtcg
      661 atgaacgagc attcggcgtc ggtcagcgcc ggcaccttcc tgcccctggc ttccgctggc
      721 atggcctcac ctgtgatcat gcgttcctac cgcgggcggc atccggtccg aatcccgtgg
      781 cccccggccc ccgtgcaaac cgtgccacaa cgccgctgcc gctgtcctgg tttccgctgc
      841 tggcagacgg cggcatgggc agccggcacc ggcggccagg ttggtggcgg gtcaggcctg
      901 ctcagctgga gggcggccgc cagcgggcat ggcgggtggc ggccgcgccc tcacggatca
      961 actggaggcg aggtcggggc ccgtcgctgc ggctggtcgg gggcctgcgc cggccagcac
     1021 gccagggctg cggccaggtg gcccccggcc cgtcgtagcc ctgctcggcg gcggcgtgca
     1081 gggtccagct ggggtcgtag agatggacgc ggccgagcgc gcagaggtcg gcgcggcccg
     1141 cgagcacgat cgagttgacg tcgtcgtagg acgagatcgc gcccaccgcg atggtggcga
     1201 tctcgacctg gttgcggatc gcgtcggcga acggcgtctg gtaggagcgg ccgaagtcgg
     1261 gccgctcgtc cggggtgacc tggccggtcg agacgtcgat cgcggcggca ccggcggcct
     1321 tgaaggcgcg ggcgacctcg accgcgtccg cgccggtgat gccgccctcg gcccagtcgg
     1381 tggcggagat gcgcaccgtc atcggcttgt gcgccggcca gaccgcgcgc atggcggcga
     1441 agacctccag cgggtagcgc agccggttgg ccagcgaccc gccgtagcgg tcgcggcgct
     1501 ggttgctgag cggcgagatg aacgacgaca gcaggtaccc gtgggcgcag tgcagctcca
     1561 ccagatcgaa cccggccgcc tccgcccgct gggtcgcggt ggcgaactgc tcccggatct
     1621 cgtcgaggtc ggcagccgtc agctcccgag gcagctggtt gaccccggga cggtagggga
     1681 ggggggaggg ggcgaccacc tcccagttgc cctcgtccag cgggtcgtcc atcccctccc
     1741 acatcagctt ggtcgagcct ttgcggccgg aatgcccgag ctggacgccg atccgggcgg
     1801 gcgactcccg gtggacgaac tcggtgatcc ggcgccacgc cgctgcctgc gcgtcgctgt
     1861 agaggccgct gcagccgggg gtgatccgcc ccgtgtcgga gacgcaggcc atctcggtca
     1921 tcaccagccc ggcaccgccc agcgccttgc cgccgagatg gacgaggtgg tagtcgttgg
     1981 gcaggccgtc gaccgcccgg tacatgtcca tcgccgacac caccacccgg ttggccagct
     2041 ccagctcgcc gagccggaac ggctggaaca tcggcggccg cgggtcgccg ggggcggttc
     2101 cctggcgccg caggtggccg gcgaaccagg cgtcgacgga cgcgacgaac tcggggtcgc
     2161 ggatccggag gttctcgtag gtgacgcggc ggctgcgggt catgatgttg aaggcgaact
     2221 gcggcggctc ctggtcggtg tactggccga ggttctcgaa ccactccagg ctggcctggg
     2281 cggcccgctg ggtcgaggcg accaccggcc ggcgctcggc ctcgtaggcg ccgagcgcgg
     2341 cctccagggt gtcctgctcg tgcaggcagg ccgccagggc gagcgcgtcc tccatggcca
     2401 gcttggtccc cgacccgatc gagaagtggg cggtgtgggc ggcgtcgccg agcagcacca
     2461 cgttgccgtg ccgccacgac tgacagcgga tcgtgcggaa tctggtccag cgggagttgt
     2521 tggccaggat ctcgtggccg tcgagcacct cggcgaacag ctcgcggatc cgcgcgatcg
     2581 acttctcgtc ggaatcgccc ggcgcgaagg tctggtcggc gtaggcgtcg aagccggccg
     2641 cgcgccacac cgactcgtgc atctcgacga tgaaggtgct gccggtggtg tcgtaggggt
     2701 agccgtggac ctgcatcacc ccgtggggcg tctccaggac atagaacttg aaggcgtcga
     2761 agacccgctc ggtgccgagc cacatgtact ggcaggcgcg cgcgtcgatg gcggggtgga
     2821 agctggcggc atagcgcgcc cggaccgccg agttgacccc gtcgcaggcg accaccaggt
     2881 cgtgggtagc cgccagctca tcgctgtccg gcgcccagcg ctggaaatgc atgctgacgc
     2941 cgagcgcctg gcagcgttcc tggagcagcc gcagcagccg tctgcggctc atcgcggcga
     3001 acccgtggcc cccgctggtg agcacctggc ctcggtagtg gacgtcgatg tcgtcccagc
     3061 gggcgaagtc ccgctccatg cgcgcgtaga ccgcggcgtc ggcgtgctcg ataccgccca
     3121 gggtctcgtc ggagaagacc acgccgaagc cgaaggtgtc gtcggcggcg ttgcgctccc
     3181 acacggtgat ctcgtgtgcc gcgtcgagct gcttggcgag cgccgcgaag tagaggccgc
     3241 cgggcccgcc gccgatcacc gcgatccgca ctggccgcct cccgcgctcg cgctatgtgg
     3301 ccggcgcggg cgcggccccg gcgcccgcct gccgcagccg gaagcgctgc agcttgccgg
     3361 tgctggtgcg cggcaggctg tcgacgaaca ccacggcacg tgggtacttg tagggggcga
     3421 tccgctgccg ggcgaactcc tgcagctccc ggatcttcgc ctcgtcgccg gccacgcccg
     3481 gtcgcagcac cacgtaggcg gtgaccagct ggccgcggtc ggcgtcgggg acgccgacca
     3541 cgccgcactc ggccacgtcg ccgtgggcca gcagcgcctc ctcgacctcc ggcccggcga
     3601 tgttgtagcc ggaggagatg atcatgtcgt cgctgcgcgc ctggtaccag aagtagccgt
     3661 cctcgtcctg gacgtaggtg tcgccggtga ggttccagcc gtgccgcacg tactcccgct
     3721 ggcgcgggtc ggccaggtag cggcagccgg tgggcccctt caccgccagc cgccccagct
     3781 gcccgggggg cagcggctgg ccggcctcgt cgaggatcgc ggcggcgtag ccgggcacga
     3841 cccggccggt cgaacccggg cggatctcgt cgccggccgt cgcgatgaag atgtgcagca
     3901 gctcggtggt gccgatcccg tcgatcagct tgacgccggt cgcctggtgg aacgcccgcc
     3961 aggtcgatcc cggcaggtgc tcgccggcgg agacccccgt ccgcaaggtc gtgagctgcg
     4021 ccgccttgcc ggcgccgagc atggcgcggt aggcagtggg ggcggtgaag cacaccgaga
     4081 cggcatgctt ggcgatcagg tccgccagct cgcccgcgct ggccttctcg atcagcaggg
     4141 tggcggcgcc ggcccgcagg gggaacacca ccagcccgcc gaggccgaag gtgaacgcca
     4201 gcggcggcgt gccggtgaac aggtcggccg ggttgggccg cagcaggtgc ctggagaagg
     4261 tgtcggcgat cgccagcacg tcgcggtgga agtgcatggt cgccttcggc cgcccggtgg
     4321 tcccagaggt gaaggcgagc agcgccacgt cgtcggcggc cgtgtcggcg tcggcgaacc
     4381 gtgccggctt ggcgtcgacc cgccggctca ggtcggcggc gtcctcgccg ccgtaggtga
     4441 ccagccgcag gcctgcgatg tccgcgccca acaggtcccc ggtgaagcgg tgatcgcaca
     4501 gcgccaggtc gacggcggcg atttcggcga tcctggtcag ctcggtgggg cgcagcagcg
     4561 gcatggtggt gaccgcgacc gcgcccgcct tgagcacgcc gaaccagcac gccaccagcc
     4621 acgggttgtt ggggccccgc agcagcacgc ggttgccggg cacgacgccg cagtcgtcga
     4681 ccagcacgtg cgccacccgg ttggcggcgc ccagcagctc gccgtaggtc cagcgctcgc
     4741 ccggcgaccg caggcactca cggtcggcgc cgtagcgttg ggccgtccgg tcgagcagct
     4801 cggtggcgca gttgagccgc tcgggatagc gcacctcggg gaggtcgaag cgaagctccg
     4861 gccactcctc ggccggcggc aggtggtcgc ggcagaacgt atcgacgtgc gctgaccgtg
     4921 tgagctgcat catcgcgcct ccccggtccc ggctccggtc cgaaccgacc agcgcttcca
     4981 gtattgccct cttgtgccgc agcgtcaccc gacccgaccc gacccggcct cgccacggcg
     5041 cggcggcagg gtgcccggcg gcgcggatcg ccgtttcgcg gaccggccgt tacaggaccg
     5101 cgaacccctg cagctcgacc agggcatcct cgtcccacag
//
LOCUS       DASWDC010000294         8995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_626558, whole genome shotgun sequence.
ACCESSION   DASWDC010000294 DASWDC010000000
VERSION     DASWDC010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8995
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626558"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1050)
                     /locus_tag="VF995_09495"
     CDS             complement(187..1050)
                     /locus_tag="VF995_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9343832.1"
                     /translation="MAEAATAPRAAAGRRPGALPLLWRQWRAQNKLFWRNPFSAFFTL
                     AFPLMFLLIFNALNTNQRITQLGDIRFAQFFTPGILVFAVVSACYVNMATSVPINRDE
                     GVLKRVRGTPLPAWIYLTARVATAVYMAFIGATLMMLVGVLAFQVKIFWHLLPAAVVT
                     MLFGTTCLCALGLAMATIVPNGEAAPAFANFTWLPIAFVSDIFYPTNNAPTWVKTAGD
                     IFPVKHFAIAMEAAFSPFTKGSGFEWNHLRVIAIWGIAAAIFAFRRFKWEPNVGGSGR
                     RVGASARYRSG"
     gene            complement(1043..2029)
                     /locus_tag="VF995_09500"
     CDS             complement(1043..2029)
                     /locus_tag="VF995_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017570771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9343833.1"
                     /translation="MGTAIKVAGLRKRYGDLEAVRGISFEVAEGECFALLGPNGAGKT
                     TTVEMLEGYRDRTEGDVRVLGTDPQHATREFRERIGIVLQSAGIHRELTVREVMALYR
                     SYYPHPRAADETIELVGLGEKAGARVKTLSGGQQRRLDLALGIIGDPDLVFLDEPTTG
                     FDPSARRRSWELIESLRALGKTILLTTHYMDEAQNLADMVTVMAGGRIVAQGPPESLG
                     GRDVAEARIRFRLPPGASVADLPGMRPNGLELEDDHVVLRTKTPTPILSILTGWAVAR
                     GEELEALTVTRPSLEDIYLQLTGTSAEAGGGAAAADAGASAGAGAGEEARNG"
     gene            2088..2603
                     /locus_tag="VF995_09505"
     CDS             2088..2603
                     /locus_tag="VF995_09505"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX9343834.1"
                     /translation="MIEHSAMRWGAPEPESVQAAARDAVTAFLAEQEREVPAERLGEG
                     RWFLRLAGERKLGIGVHLVVGDRTLRVESFFMRAPEEQRERLYHDLLVRQAGTYVLRF
                     TLDESGDLFLIGQVPLQAVTIQEVDRIVGTILEVCDTAYLPAVEIGFAASMAAEKAWR
                     ARLAERESAGG"
     gene            3081..4304
                     /locus_tag="VF995_09510"
     CDS             3081..4304
                     /locus_tag="VF995_09510"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX9343835.1"
                     /translation="MPLTSESVVSAMARPEGRKAMLLVMDGLGGYRTTQRGSELTQAR
                     TPNLDRLAREGSLGLHDPVTRGITPGSGAGHLGLFGYDPLAYEIGRGALSAAGIGFEL
                     RPGDVAARVNFCNLDGRGVITDRRAGRIPTEEAAGICELLGDRVQLDGVAFFVRPERE
                     HRGLLVLRGEGLSPEVTDTDPQREGIAPLDCEPRSPEAGAEAKRTAELLRSFLEQARE
                     LLADRQRGNFLLLRGFDTHKELPSFPERYKVRALAIAAYPMYVGISRLLGFETHQVEG
                     AAPEEVDALAPLLPAHDFVFMHVKKTDSAGEDGDFERKVAAIEEVDALIPRVLDAGVE
                     VLAVSGDHATPAQMAAHSWHPVPVLLWGGTAAADGLPKFDEVHCRQGSLGRFEAKHLM
                     PQLLAAAGRLAKYGA"
     gene            4336..5091
                     /locus_tag="VF995_09515"
     CDS             4336..5091
                     /locus_tag="VF995_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9343836.1"
                     /translation="MKGVILAGGSGTRLHPLTRITNKHLLPIYDRPMVTYAIEALVGA
                     GITEIMLVTGGTHAGEFLRLLGNGHEYGIDRLLYAYQDRPGGIAEALGLAERFVDGDR
                     VLVMLADNVVEQSIRPLVENFKGQEAGARLLLARVEDPVHLRNLGVPAFDGAGSEGGR
                     VTRIVEKPEDPPSGFAVTGIYCYEASVFEVIPTLEPSGRGELEITDVNNWYVQCGQME
                     YDVIEGFWGDAGESIDAYYLVNDFVRQHGANKG"
     gene            5210..6550
                     /locus_tag="VF995_09520"
     CDS             5210..6550
                     /locus_tag="VF995_09520"
                     /inference="COORDINATES: protein motif:HMM:NF022388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2637 domain-containing protein"
                     /protein_id="HEX9343837.1"
                     /translation="MTTTGPPLAGPPPLSSNAIASDKWLARFRTILVVILGCTVTGIA
                     SLAFYTSFEAIRDYSERSEGISPQHAWAIPLLVDSFIIVATGADLWFTTTKSRRRLFE
                     VVWPKALLAGAALVSFILNVAHAQHNWPARFVAAIPPAALVLGVELLMMVLRRSTTIR
                     SQRLQAELDARMAAEAAAAAAALPAIPPAPVRGELLRKQRTRPRREVSAPLRRTAVDD
                     FVEAAAAEAATYEPRPRPRLSDRLPDQPAERPANRSLEAERDPRAPIFGGDGGVARRP
                     PLPPKVIVPPRTATPTPGSGSNGPRPAVNGPRPGTVTQSISEPAQVQASAPGTGTGTG
                     TGTTATVTTSRTATATATATATARARREQRNNGKPATPYVVAARILDEHDESETFSPE
                     QLMQALAERGVSVNMSTAQGLLRELRSPLATRLTAQPPAAPAAGVKASKGTGRR"
     gene            complement(6629..7186)
                     /locus_tag="VF995_09525"
     CDS             complement(6629..7186)
                     /locus_tag="VF995_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX9343838.1"
                     /translation="MRQGVLYVVVCAAPPARDARVLVEFAREAGWDTCVIVTPAARAF
                     ADLAELEARSGHPVRFEWKRPGTPDVLPPADAMIVAPATFNTLNKWAAGISDTLALGL
                     LCEAIGKRLALVALPFLNAAQAYHPAFGRSLEQLREMGVRILWGEEEGVPSAHPPGTG
                     ERRIASFPWGRTLNALAETRERAPR"
     gene            complement(7272..8168)
                     /locus_tag="VF995_09530"
     CDS             complement(7272..8168)
                     /locus_tag="VF995_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX9343839.1"
                     /translation="MPGLLGLRRAVDHAWRAFQATRYSVLGRVLPELIAEAQLATDEL
                     TNDEALAATGLLAQAYQVSTITLLKLGAAELAWLSADRSIDTARRSQDELLVGGSARG
                     LVHALLEHGYYARAKELATGTAAAFESRLGRGSPALLSIYGALLLRGSVAASRQGDRS
                     ASKDLLREASIAADLLGADRNCCWTAFGPTNVAVHRVAAAVSLGDGGEAIEQGLAIDL
                     EALTILERRARFLVDLAHGYSLCAKDNEALTTLLKAERLAPEEIRLTPAVREIVGELL
                     RRQRRAGARELRALADRVGALA"
     gene            complement(8169..8531)
                     /locus_tag="VF995_09535"
     CDS             complement(8169..8531)
                     /locus_tag="VF995_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9343840.1"
                     /translation="MKEDLSVGERIAHYRRRRGMSQLVLAGRLGRSESWVSKVERGER
                     RIDRLSVIQEVAKTLDVEPIALTGRLTGRVSEGRAPGAGGRAHPQSEAEGVIEAIREA
                     FTRPDAVVRSLQRSQRRR"
     gene            8720..>8995
                     /locus_tag="VF995_09540"
     CDS             8720..>8995
                     /locus_tag="VF995_09540"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX9343841.1"
                     /translation="MAELLRQDLANAARRLFDNEAGVRLGDDPEAVHQARVGIRRLRS
                     TLRTFRSVLDREWSGALRAELAWIADLLGAVRDADVLLERLRHGLTAL"
BASE COUNT         1308 a         3169 c         3200 g         1318 t
ORIGIN      
        1 cgcaacgtca tccccttctg gtgggacatg ctgatcgtgg ccgccttcag cgtggcgatc
       61 tacgccttcg cgatgcgcgt ccgcctgccc gccgagcgcg tccgcgagta catcggcgaa
      121 gtcgagggct gaccccggaa gaagtcaggc ccgcagcgct ccagccgcgg ggtcctcagt
      181 cgctgctcag ccgcttcggt agcgggcgga ggcgccgacg cggcggccgc tgccgccgac
      241 gttgggttcc cacttgaagc ggcggaaggc gaagatcgcg gcggcgatgc cccagatggc
      301 gatgacgcgc aggtgattcc attcgaagcc gctgcccttg gtgaaggggc tgaaagcggc
      361 ctccatggcg atggcgaaat gcttcaccgg gaagatgtcg ccggcggtct tcacccaggt
      421 gggcgcgttg ttggtggggt agaagatgtc ggagacgaag gcgatcggca gccaggtgaa
      481 gttggcgaag gccggggccg cctcgccgtt ggggacgatc gtggccatgg ccaggccgag
      541 cgcgcacagg caggtcgtcc cgaacaacat cgtcacgacg gcggcgggca gcaggtgcca
      601 gaagatcttc acctggaacg ccaggacccc caccagcatc atcagggtgg caccgatgaa
      661 cgccatgtag acggccgtgg cgacccgcgc ggtcaggtag atccaggcgg gcaggggggt
      721 gccgcggacg cgcttgagca cgccctcgtc gcggttgatc gggactgagg tcgccatgtt
      781 gacgtagcag gccgacacca ccgcgaacac caggatgccc ggggtgaaga actgcgcgaa
      841 gcggatgtcg ccgagctggg tgatccgctg gttggtgttc agcgcgttga agatcagcag
      901 gaacatcagc gggaacgcca gcgtgaagaa cgccgagaag gggttgcgcc agaacagctt
      961 gttctgcgcg cgccactggc gccacagcag cgggagggcg ccagggcgcc gcccggccgc
     1021 cgcgcgaggg gccgtcgccg cctcagccat tgcgggcctc ctctccggct ccggctccgg
     1081 cgctggctcc agcgtcggcc gccgccgcgc cgccgccggc ttcggccgaa gtgccggtca
     1141 gttgcaggta gatgtcctcc agcgagggtc gcgtcacggt cagggcctcc agttcctcgc
     1201 cgcgggcgac cgcccagccg gtgaggatgc tgaggatcgg ggtgggggtc ttggtcctca
     1261 gcaccacgtg gtcgtcctcc agctcgaggc cgttcggccg catcccgggg aggtccgcga
     1321 cgctggcgcc gggtggcagg cggaagcgga tccgagcctc ggccacgtcg cggccgccga
     1381 gggactccgg cgggccctga gcgacgatcc tgccgcccgc catgaccgtg accatgtcgg
     1441 ccaggttctg ggcctcgtcc atgtagtggg tggtcagcag gatggtcttg ccgagagcgc
     1501 gcaggctctc gatcagctcc caagagcggc gccgcgccga cgggtcgaag ccggtggtgg
     1561 gctcgtccag gaagaccagg tcggggtcgc cgatgatgcc gagcgccagg tcgaggcgcc
     1621 gctgctgccc gccggacagg gtcttgacgc gggcgccggc cttctcgccg agaccgacca
     1681 gctcgatggt ctcgtccgcc gcgcgcgggt gcgggtagta gctgcggtag agggccatga
     1741 cctcgcgcac ggtcagctcc cggtggatgc cggccgactg cagcacgatg ccgatgcgct
     1801 cgcggaactc ccgggtggcg tgctgagggt cggtgccgag cactcggacg tccccttcgg
     1861 tgcggtcgcg gtagccctcc agcatctcca ccgtggttgt cttgccggcg ccgttcggcc
     1921 ccagcagggc gaagcattcg ccctccgcca cctcgaacga gatgcccctg accgcctcca
     1981 ggtcgccgta gcgtttgcgc agcccggcga ccttgatggc tgttcccacc ggtttcacat
     2041 ccttccaaag gggtttagcg cccattgggc cgctacagtc tgtggtcatg atcgaacatt
     2101 ccgcaatgcg ttggggcgcc cccgaaccgg aatccgtcca ggcggcggca cgcgacgcgg
     2161 tgacggcgtt cctggcagag caggaacggg aggtgccggc cgagcggctc ggcgaggggc
     2221 gctggttcct gcgcctggcg ggggagcgca agctcggcat cggggtccac ctggtggtgg
     2281 gggaccgcac cctgcgggtc gagtcgttct tcatgcgcgc tccggaggag cagcgcgagc
     2341 gcctctacca tgatctgctg gtgcgccagg ccggaaccta tgtgctgcgg ttcaccctgg
     2401 acgaatccgg cgacctgttc ctgatcggtc aagtgccgct ccaagctgtt acaattcagg
     2461 aagttgaccg aatcgttggg acgattctag aggtctgcga taccgcctat ttgcccgcag
     2521 tggaaatcgg gttcgcagcg agcatggcgg ccgaaaaagc ctggcgcgcc cgcctggcgg
     2581 aacgggaatc agctggcggg taaattccat ggatgaaatt ctaccgcctc tacgctgctg
     2641 atacgccgct gacctgcggc gatacgcgct tgacggcggc ttggccagct tttagatttc
     2701 ccactgtcct gcgggacgca gtgcggtagg gtcaggaaaa ctcgactgga ggattcagtc
     2761 ggggcaagct gaaaagcgcg ccccgctgcg tcggtggatc ccggtgggtc tgcgcaaccc
     2821 gccgacgtac cggcgaaatt ccggcggctt tggccgcggg aatttagctc cggccgagcg
     2881 ggagcgatct gatctgtgct cgtccgcagg gctccggtcc ccaggggccg ttcgccggga
     2941 gctggcggct tgccgtcagc aaccgagcgg gctcctgggg accgttcttt tcgcgcgccc
     3001 gctgcggcgg tctgttcccc ggtccgtggg tagactcgat ctgacttgtg acctgcccac
     3061 gttcttcccg gaggatcttg atgccgctca ccagcgagtc cgtcgtcagc gcgatggcca
     3121 gacccgaggg ccgcaaggcg atgctgctcg tcatggacgg gctcggcggc taccggacga
     3181 cgcagcgggg ctcggagctg acgcaggcac gcacacccaa cctcgaccgc ctggcacgcg
     3241 agggatcgct cggcctgcac gacccggtca cccgcgggat cacgcccggg tccggggccg
     3301 ggcacctggg cttgttcggc tacgacccgc tcgcctacga gatcggcagg ggcgcgctgt
     3361 cggccgccgg gatcggcttc gagctgcggc ccggcgacgt ggccgcgcgg gtcaacttct
     3421 gcaacctgga cgggcgtggg gtcatcaccg accggcgcgc cggccggatc cccaccgagg
     3481 aggccgctgg catctgcgag ctgctcggcg accgggtcca gctggacggc gtggcgttct
     3541 tcgtccggcc ggagcgcgag caccgcggcc tgctggtgct gcgcggcgag gggctctcac
     3601 ccgaggtcac cgacaccgac ccgcagcgtg agggcatcgc gccgctcgac tgcgaacccc
     3661 gctcgccgga ggccggcgcc gaggcgaagc gaaccgccga gctgctgcgc tcgtttctgg
     3721 agcaggcccg cgagctgctc gccgaccgcc aacgcggcaa cttcctgctc ttgcgcggct
     3781 tcgacaccca caaggagctg cccagcttcc ccgagcgcta caaggtgcgg gcgctggcga
     3841 tcgccgccta cccgatgtac gtcggcatct cccgtctgct cggcttcgag acccatcagg
     3901 tggagggcgc ggcgcccgag gaggtcgacg ccctcgcgcc gctgctgccc gcgcacgact
     3961 tcgtcttcat gcacgtcaag aagaccgact cggccggcga ggacggcgac ttcgagcgca
     4021 aggtggcggc gatcgaggag gtggacgcgc tcatcccgcg cgtgctcgac gccggggtcg
     4081 aggtgctggc ggtgagcggc gaccacgcca ctcccgcgca gatggcggcg cactcctggc
     4141 atccggtccc ggtgctgctg tggggcggca ccgccgcggc cgacggcctg cccaagttcg
     4201 acgaggtcca ctgccggcag ggctcgctcg gccggttcga ggccaagcat ctgatgccgc
     4261 aacttctcgc ggcggccggc cgtctggcaa agtacggcgc ctgagaccta accgaccgga
     4321 cggaggggta ggcaagtgaa aggtgtcatc ctggcgggcg ggagcgggac caggctgcat
     4381 ccgctgacac gcatcaccaa caagcacctg ctgccgatct atgaccggcc gatggtcacc
     4441 tacgcgatcg aggcgctggt cggcgccggg atcaccgaga tcatgctggt caccggcggc
     4501 acccacgccg gcgagttcct gcgcctgctc ggcaacgggc acgagtacgg catcgaccgg
     4561 ctgctctatg cctaccagga ccgccccggc ggcatcgccg aggcgctggg gctcgccgag
     4621 cgcttcgtcg acggggaccg ggtgctggtg atgctcgccg acaacgtggt cgagcagtcg
     4681 atccgcccgc tggtcgagaa cttcaagggc caggaggccg gcgcgcgcct gctgctcgcc
     4741 cgcgtcgagg acccggtgca cctgcgcaac ctcggcgtcc cggcgttcga cggagcgggc
     4801 agcgagggcg gccgcgtgac ccggatcgtg gagaagccgg aggacccgcc gagcgggttc
     4861 gcggtcaccg ggatctactg ctacgaggcg agcgttttcg aggtgatccc gaccctggag
     4921 ccgtcaggcc ggggggagct ggagatcacc gacgtgaaca actggtacgt ccagtgcggg
     4981 cagatggaat acgacgtcat cgagggcttc tggggcgacg ccggcgagtc gatcgacgcc
     5041 tactacctgg tcaacgactt cgtccgccag cacggcgcca acaagggctg aacctggaaa
     5101 atccaccggc aaaccgtccg tagccagtcg gttggtcacg aaggccgcgc gtgttgtaaa
     5161 acgtcagcct gcatgtcttg cctcagcctc cacgttccga ggagtcctcg tgaccactac
     5221 tgggccgccg cttgctggac ccccgccgct ctcctccaac gccatcgcat ccgacaagtg
     5281 gctggcccgg ttccggacca tcctggtcgt gatcctgggc tgcaccgtga ccggcatcgc
     5341 ctcgctcgcc ttctacacca gcttcgaggc gatccgtgac tactccgagc gctccgaggg
     5401 catctcgccc cagcacgcct gggcgatccc gctgctggtg gactcgttca tcatcgtggc
     5461 caccggcgcc gacctgtggt tcaccaccac gaagtcgcgg cggcggctgt tcgaggtggt
     5521 ctggccgaag gcgctgctgg ccggcgcggc gctggtcagc ttcatcctca acgtcgccca
     5581 cgcgcagcac aactggcccg cccgcttcgt cgccgcgatc ccgccggccg cgctggtcct
     5641 cggcgtcgag ctgctgatga tggtcctgcg gcgcagcacc acgatccgct cccagcggct
     5701 ccaggccgaa ctggacgccc ggatggccgc cgaggccgcc gccgcggccg cggcgctccc
     5761 ggccatcccg ccggcgccgg tgcgtggcga gctgctccgc aagcagcgca cccggccgcg
     5821 ccgcgaggtg agcgcgccgc tgcgccgcac ggcggttgac gacttcgtgg aggccgcggc
     5881 cgccgaggcg gcgacctacg agccgcggcc tcgcccgcgg ctgtccgacc ggctccccga
     5941 ccagcccgcg gagcggccgg cgaaccggtc gcttgaggcc gagcgtgacc cccgcgcgcc
     6001 gatcttcggc ggcgacggtg gcgtggcgcg gcgcccgccc ctgccaccga aggtgatcgt
     6061 cccgccccgg accgccacgc ccacgcccgg gagcgggagc aacggcccgc gtccggccgt
     6121 gaacgggccg cgcccgggca ccgtcaccca gtcgatctcc gagccggccc aggtccaggc
     6181 ctcggccccg gggacgggga cggggacggg gacggggacg accgcgaccg tcaccaccag
     6241 ccgaacggcc accgccaccg ccaccgcaac ggccacggcg agggcgcggc gcgagcagcg
     6301 gaacaacggc aagccagcga ccccgtacgt ggtggccgcg cgcatcctcg acgagcacga
     6361 cgagagcgag accttcagcc cggagcagct catgcaggca cttgccgagc gcggtgtctc
     6421 ggtgaacatg agcaccgcgc agggcctcct gcgcgagctg cgctcgccgc tggccacccg
     6481 cctcaccgcc cagcccccgg cggcgccggc cgcgggcgtg aaggcgagca aggggactgg
     6541 ccgtcgctga tcgctgaagc cgccaattca accacgctag gtggttgaat tggcggcttc
     6601 agtcaccaac aaaccaggac agcttgtcct atcgcggtgc gcgttcgcgc gtctcggcga
     6661 gggcattcag ggtgcgtccc caggggaagc tggcgatgcg ccgttcgccg gtgcccgggg
     6721 gatgcgccga gggcacgccc tcctcctcgc cccagaggat ccgtacgccc atctcgcgga
     6781 gctgctccag gctccgcccg aaggccgggt ggtaggcctg cgcggcgttg aggaagggga
     6841 gcgccaccag cgcgaggcgc ttgccgatcg cctcacacaa gagccccagg gcgagcgtgt
     6901 cgctgatccc ggcagcccac ttgttgagcg tgttgaaggt ggccggggcc acgatcatgg
     6961 cgtcggcggg cggcaggacg tccggcgtgc caggccgctt ccactcgaac cggaccgggt
     7021 ggccgctgcg cgcctccagc tccgccaggt cggcgaacgc ccgcgcggcc ggcgtgacga
     7081 tcacgcaggt gtcccaaccg gcctcgcggg cgaactccac cagcaccctc gcgtcacgcg
     7141 cggggggtgc ggcacacacc accacataca ggacgccctg tctcatcagg gggctgccgc
     7201 tcggctgacc tggaccaccg gattctctgg ccggggcctc gctcccccgg cccccggccc
     7261 ccctccccgc atcaggccaa ggctcccacg cgatccgcga gcgcccgcaa ctcccgcgcg
     7321 ccggcgcggc gctggcggcg cagcagctcg cccacgatct cgcgcacggc tggggtgagg
     7381 cggatctcct cgggggcgag gcgttccgcc ttgagcaggg tggtgagggc ctcgttgtcc
     7441 ttggcgcaga ggctgtagcc atgtgccaga tcgaccagga agcgtgcccg gcgctccagg
     7501 atcgtcagcg cctccaggtc gatcgccagg ccctgctcga tcgcctcgcc gccgtcgccg
     7561 agcgagacgg ccgcggcgac ccggtgcact gccacgttgg tcggcccgaa cgcggtccag
     7621 cagcagttgc ggtcggcgcc gagcaggtcg gcggcgatcg acgcctcgcg cagcaggtcc
     7681 ttgctggccg agcggtcgcc ctgccgcgac gccgccaccg atcctctgag cagcagcgcg
     7741 ccgtagatcg acagcagcgc cggggagccg cggccgagtc tcgactcgaa ggcggcggcc
     7801 gtgccggtgg ccagctcctt ggcgcgagcg tagtagccgt gctccagcag ggcatggacc
     7861 aggccgcggg cgctgccgcc gaccagcagc tcgtcctggg accgcctggc cgtgtcgatg
     7921 ctgcggtcgg ccgacagcca ggctagttcg gcggcgccga gcttcagcag cgtgatggtg
     7981 ctgacctggt aggcctgcgc cagcaggccg gtggcggcca gcgcctcgtc gttggtcagc
     8041 tcgtcggtgg ccagctgggc ctcggcgatc agctcgggca ggactcggcc aagtaccgag
     8101 tagcgggtgg cctggaacgc ccgccaggca tggtcgacgg cccgccgcaa ccccagcagg
     8161 ccgggcacct agcggcggcg ctggctacgt tgcagcgacc gcaccaccgc gtcggggcgg
     8221 gtgaacgcct cacggatggc ctcgatcaca ccttccgcct cgctctgcgg atgggcccgc
     8281 ccgcccgcgc cgggcgcgcg cccctcgctc acgcgaccgg tcaggcgtcc ggtcagcgcg
     8341 atgggttcga cgtcgagggt cttggcgacc tcctggatca ccgagagccg gtcgatacgc
     8401 cgctccccgc gctccacctt ggagacccag ctctccgagc gccccaaacg cccggcaagc
     8461 accagctgcg acatcccgcg tcggcgccgg tagtgcgcga tgcgctcacc gactgacagg
     8521 tcctctttca cgagcacccc cctggaccgg tggtctggcc gtcaggacca gaccaattca
     8581 atagcattga tgctttgttg gacccgatcc gtcaaattgt gattcggggc gcagatggct
     8641 cgccactgtg cgtggttgcg atacggtaat gggaatgcgc ggtgggttgg agccggacac
     8701 gagccaggag attctctcca tggcggaatt gctccgccag gatctcgcca acgctgcccg
     8761 caggctgttc gacaacgagg ccggggtgcg gctgggcgac gaccccgagg ccgtccacca
     8821 ggcgcgtgtc ggcatccgcc ggttgcgctc cacgctgcgg accttccggt cggtgctcga
     8881 ccgggagtgg agcggcgccc tgcgcgccga gctggcctgg atcgccgatc tgctgggcgc
     8941 ggtccgcgac gccgacgtgc tgctggagcg cctgcggcac ggcctgaccg ccctg
//
LOCUS       DASWDC010000295         3271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_960711, whole genome shotgun sequence.
ACCESSION   DASWDC010000295 DASWDC010000000
VERSION     DASWDC010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960711"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VF995_09545"
     CDS             <1..316
                     /locus_tag="VF995_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9343842.1"
                     /translation="WRLYDTHYTERYLGDPSADPGVYQRSSLLADAARLERPLLLIHG
                     LADDNVVAAHTLRLSGLLLAAGRPHSVLPLTGVTHMAVQEAVAENLLHLQLAFLRAAL
                     GL"
     gene            complement(332..1372)
                     /locus_tag="VF995_09550"
     CDS             complement(332..1372)
                     /locus_tag="VF995_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9343843.1"
                     /translation="MRLGVSIGYWGAGVDAARQLAAAREAERLGYDAVWAAEAYGSDA
                     ATVLAWVAAQTSTIGLGAAVFQVPARSATMAAMTAATLDQLSGGRFRLGLGLSGPQVA
                     EGWYGQRYDHPLTRMRDYATVVRTALARQRVDYQGETIQLPLPGGPGKPLKLTISPRQ
                     RPLPLYFGAMGPRNLALTGELADGWLAFLLDPTQMATFRRSLDEGAARVGRDLAADGF
                     DIAPTVQFQVSDDHDAARDAMRPFLALYVGGMGSRERNFYNRLVRSYGFEDAAATVQD
                     HFLAGRWGDAAAAIPEALVDAVCLCGPAAHIRDRLAAYRDAGVTNLILSPVASTTDAL
                     IAQLRRAVELAG"
     gene            complement(1456..2838)
                     /locus_tag="VF995_09555"
     CDS             complement(1456..2838)
                     /locus_tag="VF995_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003780726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9343844.1"
                     /translation="MGQRPDQERISALTAARTQLVHERTPKSREWRERSLKSLPLGVA
                     SSFQDSPPYPIFFATAKGSRVWDVDGNEYSDYHNGFGVMSVGHAHPKIAAALSERAGI
                     GTHFAQPVAETTLLAEELCQRFQLDQVRFTNSGTESTMDAIRLARGVTGRDKIVKIEG
                     SYHGHHESVLVSIKPDRDAIGPRAHPASVPLGKGTPRAVTDLTLIVPFNDADALDRLL
                     AANTGEVAALIMEPIMMNVGIIDPDPGYLEAVREVCTRHGVLLIWDEVKTGATVAYGG
                     AEELYGVSPDLKCFAKAVGGGTPLGAFGGRGELMAEIAREGVPQLGTFNGNPLSARAG
                     LVALTEVLTPDAYKELNRVNEALLGGCQRIIDDHDLPCYTIGMGGKGAVMYAPEELHD
                     YRDYAGFDAAGVDADLSYLAWLYELTEGVFMTPGTDEQWTLSVQHTDEDVARYVNAFE
                     TFAREVTAAG"
     gene            2998..>3271
                     /locus_tag="VF995_09560"
     CDS             2998..>3271
                     /locus_tag="VF995_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEX9343845.1"
                     /translation="MPTRGSGAPLDPVDRRIVAALQQDGRRPFTRIARDLGISEAAVR
                     QRVARLESAGVMQVVAVADPITCGFKTMAMLAIRVEPRAIAQVAEAL"
BASE COUNT          439 a         1066 c         1278 g          488 t
ORIGIN      
        1 ctggcgcctg tacgacaccc actacaccga acgctacctg ggcgatccca gcgccgaccc
       61 gggcgtctat cagcgcagct cactgcttgc cgacgccgct cgcctggagc gccccctgct
      121 gctgatccac ggcctcgccg acgacaacgt ggtcgccgcc cacacgctgc gcctgtcggg
      181 cctgctgctc gccgccggcc gcccgcacag cgtcctgccg ctgaccggcg tcacccacat
      241 ggcggtccag gaggccgtcg cggagaacct cctgcacctg cagctggcat tcctgcgcgc
      301 ggcgctgggg ttgtgaccgg gcctgccggg cctaccccgc cagctcgacg gcgcggcgga
      361 gctgggcgat gagcgcgtcg gtggtggagg cgaccgggga gaggatcagg ttggtgaccc
      421 cggcgtcgcg gtaggcggcg aggcggtcgc ggatgtgggc ggcggggccg cagaggcaga
      481 ccgcgtcgac cagggcttcg gggatggcgg cggcggcgtc gccccagcga ccggcgagga
      541 agtggtcttg gacggtggcg gcggcgtcct cgaagccgta ggagcggacc aggcggttgt
      601 agaagttgcg ctcgcgggag cccatgccgc cgacgtagag agcgaggaac gggcgcatcg
      661 cgtcgcgggc ggcgtcgtgg tcgtcgctga cctggaactg gacggtcggg gcgatgtcga
      721 agccgtcggc ggcgaggtcc cggccgacgc gggcggcgcc ctcgtccagg gagcggcgga
      781 aggtcgccat ctgggtgggg tcgagcagga acgccagcca cccgtcggcc agctcgccgg
      841 tgagggcgag gttgcgcggc cccatggcgc cgaagtagag cggcaggggc cgctggcggg
      901 ggctgatggt gagcttgagc ggcttgccgg ggccgccggg gagcgggagc tggatggtct
      961 cgccctggta gtcgacccgc tggcgggcga gcgcggtgcg gaccacggtg gcgtagtcgc
     1021 gcatccgtgt cagcgggtgg tcgtagcgct gcccgtacca gccctcggcg acctgtgggc
     1081 cggacaggcc gaggccgagc cggaagcgcc cgccggagag ctggtcgagc gtcgcggcgg
     1141 tcatcgcggc catggtggcg ctgcgcgcgg ggacctggaa caccgccgcg cccagcccga
     1201 tcgtgctggt ctgcgcggcg acccaggcga gcaccgtggc cgcgtcggat ccgtaggcct
     1261 cggccgccca gacggcgtcg tagccgagcc gttccgcctc ccgtgccgcc gcgagctgcc
     1321 gcgccgcgtc gaccccggcg ccccagtagc cgatgctgac gccgagccgc accgcacccc
     1381 tccttactgg tcgttcagcg gagcgtagca gcacaaccgg accgagccac gggacttcgt
     1441 cggtatggag gcaaatcacc cggctgccgt gacttcccgc gcgaacgtct cgaaggcgtt
     1501 gacgtagcgg gcgacgtcct cgtcggtgtg ctgcaccgac agggtccact gctcgtcggt
     1561 accgggggtc atgaagacgc cctcggtcag ctcgtagagc caggcgaggt aggacaggtc
     1621 tgcgtcgacc ccggcggcgt cgaagccggc gtaatcgcgg tagtcgtgca gctcctccgg
     1681 ggcgtacatg accgcgccct tgccgcccat gccaatggtg tagcagggca ggtcgtggtc
     1741 gtcgatgatc cgctggcagc caccgagcag cgcctcgttg acgcggttca gctccttgta
     1801 ggcgtcgggg gtgagcacct cggtgagggc gaccaggccg gcgcgggccg acagcgggtt
     1861 gccgttgaag gtaccgagct gggggacgcc ttcgcgggcg atctcagcca tcagctcgcc
     1921 ccggccgccg aaggcgccga ggggggtgcc gccgccgacc gccttggcga agcacttgag
     1981 gtcggggctg acgccgtaga gctcctcggc gccgccgtag gcgacggtcg cgccggtctt
     2041 gacctcgtcc cagatcagca gcacgccgtg gcgggtgcag acctccctga ccgcctccag
     2101 atagccgggg tcggggtcga tgatgccgac gttcatcatg atcggctcca tgatgagcgc
     2161 ggcgacctcg ccggtgttgg cggccagcag ccggtcgagg gcgtcggcgt cgttgaaggg
     2221 gacgatcagg gtcaggtcgg tgaccgcccg cggggtgcct ttgccgaggg ggacgctggc
     2281 ggggtgggcg cgcgggccga tggcgtcgcg gtcgggcttg atcgacacca gcaccgactc
     2341 gtggtgcccg tggtaggagc cttcgatctt gacgatcttg tcgcggccgg tgacgccgcg
     2401 ggcaagtcgg atggcatcca tggtcgactc ggtgccggag ttggtgaagc ggacctggtc
     2461 gagttggaag cgctggcaca gctcctcggc gagcagggtg gtctcggcga ccggctgggc
     2521 gaagtgggtg ccgatgccgg cacgttcgct gagcgccgcg gcgatcttgg ggtgggcgtg
     2581 gccgaccgac atgacgccga agccgttgtg gtagtcggag tactcgttgc cgtcgacgtc
     2641 ccagacgcgg ctgcccttgg cggtggcgaa gaagatcggg tacggcgggg agtcctggaa
     2701 gctggaggcg acgccgaggg gcagggactt gagggagcgt tcgcgccact cccgcgactt
     2761 gggggtccgt tcgtggacga gctgggtacg ggcggcggtg agcgccgaga tgcgctcctg
     2821 gtcgggccgc tggcccatga tgatcacgct cctgcgccgg tggccggtgg ccgggtggtt
     2881 gctcgtgaag gaacactgaa tgcgctgcct tccgaaagta cttcgagatc cgtgctatgg
     2941 tcaaccatct ttgaaagatt ccgttgcgag tcgagcggat caggaggtag ttcgtaggtg
     3001 cctacgcgcg ggagcggcgc tccgctcgac ccggtcgacc gccggatcgt ggcggcgctg
     3061 cagcaggacg gccgccggcc cttcacccgg atcgcccgcg acctcggcat ctcggaggcc
     3121 gccgtccgcc agcgggtcgc ccggctggag agcgccggcg tgatgcaggt ggtcgcggtc
     3181 gccgacccga tcacctgcgg cttcaagacg atggcgatgc tggcgatccg ggtcgagccg
     3241 cgtgccatcg cccaggtcgc cgaggcgctg c
//
LOCUS       DASWDC010000296        15715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1500047, whole genome shotgun sequence.
ACCESSION   DASWDC010000296 DASWDC010000000
VERSION     DASWDC010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15715
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500047"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..243)
                     /locus_tag="VF995_09565"
     CDS             complement(10..243)
                     /locus_tag="VF995_09565"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HEX9343846.1"
                     /translation="MANLTLSVDDELLQRARMRALELHTTVNALVRDYLESFAGADPT
                     RQAISEFLNIAGTVHASSGPKGRTWKREDLYDR"
     gene            complement(292..1179)
                     /locus_tag="VF995_09570"
     CDS             complement(292..1179)
                     /locus_tag="VF995_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX9343847.1"
                     /translation="MDRPIVVADRIATASAVVALESSVLAQGLPSPASLESAEAQSLA
                     VSKSGAEPAVVAVINGRIVVGADPDQVELLATARTTWKVTVRDLPYVVARRLDGATTV
                     SATVFAAERVGIDVMATGGIGGVHFGANDVSADLPQLARSRLVVVCSGAKHVADSAAT
                     LEWLETNGVLVVGYRTDRMPGFLARGNLPLEHLVNNVEEAVELVRVRRALDSPGALLL
                     VHNPPASAAMDPDELAELAAASSVAARDAGIVGKARTPFELDWLHRRTGGRSLAANLA
                     LLEANAGLAGELAAALAGS"
     gene            complement(1327..4458)
                     /locus_tag="VF995_09575"
     CDS             complement(1327..4458)
                     /locus_tag="VF995_09575"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEX9343848.1"
                     /translation="MPHSRPRGSAAPEPGPFLARLGFEEPLAVVGDLEALGAWPPHAA
                     AGGLRLLHELGGSANPELATRALGAVAAALPEGDRGRFTAALRRQLGFRRRLVALVAA
                     SRSLGAWLAAHPEEVDRLADGRAFAAPRTRDELVEEALATVSAPDPVADPVADPVADP
                     VADPVVAWEALRRFKRRELLRIAVRDLAGGAAVEVVGQELAALAEACLEAALAVVAVV
                     GRRVEEAERVRVAVLGMGKLGGEELNYVSDIDVLFVHEPAPRPASGVEDEGEADADEA
                     ARIAAAIARELIGGLSAVTAEGTCYRVDANLRPEGRNGPLTRTLGSYQAYWDRWAEAW
                     EQQALIKVRPVAGNPALGERFHQAAAAWTYPERLDPEAIAAIRHMKARVEASQQARDG
                     GERQVKLGPGGLRDVEFAVQLLQLVHARHDPELRSGSTLVALEALSAAGFVGRSDAAA
                     LASAYRFLRMIEHRLQLASERRTHTIPAGEGERRWLARTMGYRDGPDGDALELFEADR
                     RRHAATVRSLHEKLFYRPLLEVFGAVPTGLDPREARERLAALGFANPERALAHLRSLT
                     SGLSRRATLFRAMLPAMLTWLADAPDPDSGLAALRVVAEALGRHGAFLATLRDNPAVA
                     ELLCIVLGSSRLLGDLLARHPELLTAMAEAGGPAGSRTHDQLVGEALAIVTRHLGPGH
                     DGDDRQVAAAWDGLRRFKRRALLRIAVRDLAGGVAVEVVGQELATLAEASLEAGLAVA
                     SHRVRGAEQVRVTVLGMGKLGGEELNYVSDLDILLCHEPADGADPEQAARAAAQIARE
                     LLNGLGAVTAEGTGFKVDANLRPEGRNGPLTRTLASYQAYWDRWAEPWERQALIKVRP
                     VAGDRALGLRFGQAAAAWTYPERLDAEQVAAIRRMKARVESERLPAGADPRLHLKLGP
                     GGLADVEWTVQLLQLRLGGTHPEVRRQGTFAALDALTAARALDAREAAWLAGAYRLCL
                     RLRNHGYLVAGRPVDSLPTDPLLLERLAETMGEPPGGRQRLLESFRRATRRARRVVMA
                     RFWDEHPVSGTRG"
     gene            complement(4608..5336)
                     /locus_tag="VF995_09580"
     CDS             complement(4608..5336)
                     /locus_tag="VF995_09580"
                     /inference="COORDINATES: protein motif:HMM:NF020366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HEX9343849.1"
                     /translation="MGANSGWDDSSGWDDEPDSSWRAPEASPASGRAARPPSPSRRRR
                     SPLVTLVGVVALVVALMVVARAPVTRTFHPAHVAHPALGRPVRVVAAGAGAGREYAFP
                     LPSGWRDQTRQLAGSYRGVRPVQVLTGRATSGFVADLSVLRQPAAQPPSLPRLAAALP
                     GRLRDTLGAVPVGSAWPLQLDSAAAIATDWTLAQGGHPVRVRQIACYHHGAVWWLTFS
                     ASTQAFPGDVAALDRAVRAWRWTT"
     gene            complement(5391..6710)
                     /locus_tag="VF995_09585"
     CDS             complement(5391..6710)
                     /locus_tag="VF995_09585"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX9343850.1"
                     /translation="MDKQREYVLRMVEERDIRFVRLWFTDVLGFLKSFAITPAELEEA
                     FEQGMTFDGSAIEGYARAQEADVVARPDPSTFQVLPGRTGGDGGVARMFCDVLTTEGE
                     SFDADPRWVLRRNLQRAADRGFTVYVQPELEFFLFKNAKSVRPLDSGSFFEQTALDLA
                     HDFRQDAIDMLERMGISVMQSHHEIAPSQHEIDLREADALTMADNVMSCRLVVKEAAL
                     ERDLHASFMPKPIQEVDGSGMDTHLSLYEGDRNAFYDPGQEANLSKTARSFIAGLLVH
                     AREYTAVTNQWVNSYKRLVPGFDAPVHIAWAQRSRSAMVRVGGYRSDRVDVEVRSPDP
                     AANPYLAFAVLLAAGMKGIEEDYELPPEADDALDRMTEAERRAAGIEPLPVDLHEALV
                     AMESSELVAEALGEHVFEYFLRNKRRDWQAYQRYVTPFERKRYLPLL"
     gene            6817..8592
                     /locus_tag="VF995_09590"
     CDS             6817..8592
                     /locus_tag="VF995_09590"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX9343851.1"
                     /translation="MERLRLAGAQLNQVVGDIDGNVERILAIYREAAAKGAHLAVFPE
                     LAVTGYMPEDLVFKPDFLAASQAGIERVAAGVGACAAVVGFVEATPAGPANAAALCHD
                     GRILATYHKVLLPNYSVFDEERYFIPGREVLLASFGEVSVAITICEDLWFPWGPMAAA
                     AAAGAEVVVSINGSPYRLGKADRVGPMLATRAADHGAHLFYVNRVGGQDELAYEGESC
                     YFDPEGRVRARAASFEEDLLLVDLTLEEGATARLADRPRRTPASVPPGAGSPPSLAVR
                     RVQVTDRLPDPGPLVERRLSPRLGAEEEVYRALVCATRDYVTKNGFGAVHVGLSGGID
                     SSLVAAIAVDALGPDRVTGVAMPSSYSSEGSLRDAQALAANLGIELLTLPIHDPVDGF
                     AAALKEPFAGTEPGLAEENIQARVRGTLLMSLSNKFGTMVLTTGNKSEMAVGYCTLYG
                     DMAGGFAVIRDVPKTLVYALARWRNQQAHARGAGEVIPQSVLDKPPSAELRPGQLDTD
                     SLPPYEVLDPILERYIEDDASTDELVAEGFDRAVVERVVGLVDGAEYKRRQAPPGPKI
                     TGRSFGKDRRLPISNRFRGYPRAAR"
     gene            8665..9990
                     /locus_tag="VF995_09595"
     CDS             8665..9990
                     /locus_tag="VF995_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9343852.1"
                     /translation="MGSGSEGGGVEGLAGGSRWRRWRRLAVVDNRPLRERRDFRLLFM
                     AQGGSTFGTRITYVAIPYQVFRITRSPLAVGLLGLAELTPLLLTALLGGALADAHDRR
                     RMVRLTELGMATASASLLVNAALPHPALWVLYAASVVLAALDGLQTPSLDALVPRLVE
                     RDELPAAVALEGLRGTLGMVAGPALGGVLVATAGLPVAYGVDVATFGFSLLLLSRMRA
                     VPPPPGAARVSLRGILDGLRYARSRPELLGTYVVDIVAILFGMPLALFPAIADRYGGA
                     GVLGLLYAAPSAGALLANLTSGWLGRSRRHGAGILGAAGCWGLAIAAFGVAYGLGGPL
                     WLALLLLAASGGADMVSGLFRSTIWNQTIPDELRGRLGGIELLSYSIGPTLGNVEAGA
                     VASAFTVTTSVVSGGALCVAGVGVCALLLPAFRRYDAARFLAAARANAA"
     gene            10079..10870
                     /gene="panB"
                     /locus_tag="VF995_09600"
     CDS             10079..10870
                     /gene="panB"
                     /locus_tag="VF995_09600"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX9343853.1"
                     /translation="MPVTITDLAAWKERGERFVMLTAYDALTARVLGEAGVPLLLVGD
                     SLGNNVLGYDSTIPVTMEESLVFTAAVARGARNAMVVGDLPFGSYQAAAADAMRNAAR
                     LVKVGANAVKLEGGQRSVEAVRLIVAAGVPVMGHLGLTPQSANSFGGFRVQGRGEAGD
                     RLLADALSLQAAGCFSIVLEAIPAELGKRVTEALQIPTIGIGAGPHTDAQVLVITDLL
                     GLTPDPAPRFVKRYADLRSTIAEAAQRFTKDVAEGHYPGPEHQYH"
     gene            10967..11548
                     /locus_tag="VF995_09605"
     CDS             10967..11548
                     /locus_tag="VF995_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9343854.1"
                     /translation="MSEAPTVPSGSRVVDGLVVPEAGTWVADAAHSTFEFVARHLTAK
                     VRGRFHTATVTAHVAEVPEQSTVEVEIDTASVDTHEPRRDGHLKSPDFFNVEKYPKMT
                     FRSTGLRSAGEGVWELTGDLTMLDATRPVSFDLEFLGTSPDPWGGIRAGFSAFAEVDR
                     EDWGLNWNSALETGGWLLSKKIRLEIEVEAVRQ"
     gene            complement(11538..13238)
                     /locus_tag="VF995_09610"
     CDS             complement(11538..13238)
                     /locus_tag="VF995_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9343855.1"
                     /translation="MARLPAVLRSRRARAAVLAAVAVTIALLPADFALLRMQHHKLEA
                     SIQSTARTDVLRVARLLRTGAPAEELLHGAPGDLIQVVDTAGLVSLESPALHGAPALA
                     AAGQPLRGDTVSVAHLRRLPAVVADGRGPYLMLSTRVTTPREGARIVFVAASLHLAEL
                     SEHTTQTGLDLQTPLLILLVAVAAWFLAAHALRPVEAIRVEAGEIARRDLSLRIADQR
                     AAGEVVPLVRSMNELLEQLEASAARQRRFVADASHELRSPLTAIQLQLDVALALPERT
                     DWRAVADAVMQEVRRMQRIVEDLLLLARVDEATLPLRRDQVDLDELALAEARRLRNRG
                     KVMVDTSGISAARVTGDRHRLTQVARNLAANAERHARQQVRLEVRRLDGGEAELVVAD
                     DGPGIPPEDRERVFERFTRLDEARSPDRGGAGLGLAIVREIVQAHGGTVAVAVAGQGS
                     NDPNDSGGSGARLVVRLPADGPSDGHAEGHEPTERPELGGYPAGSRGSPALLGSSQRP
                     LRSVQLRLSRAVARLSGRQRPLRLPQVPVAADWAAAPMARVPPGCRRRRPPARRPPTA
                     "
     gene            complement(13231..13908)
                     /locus_tag="VF995_09615"
     CDS             complement(13231..13908)
                     /locus_tag="VF995_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9343856.1"
                     /translation="MRVLVVEDERPLAETLRRGLEAEGFVVEVAHDGNDGLWRAQEHP
                     FDAIVLDIMLPGLNGYRVCAALRDAEIWTPVLMLTAKDGDLDEAEALDTGADDYVTKP
                     FSFVVLVARLRALLRRGPRERPTVLSAGDLRLDPARHHLDRSGTEISLTAREFALLEL
                     LMRHPGEVLAKRQILDEVWDLEFDGDPNIVEVYIGYLRRKIDVPFGRNAIETVRGVGY
                     RLAADGG"
     gene            complement(13916..>15715)
                     /locus_tag="VF995_09620"
     CDS             complement(13916..>15715)
                     /locus_tag="VF995_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX9343857.1"
                     /translation="QSAPIADRLAAHTCLAQVLAGEHRADQRAWHLAAAATGPDEQVA
                     SALERTADRAARRGGTVAAADALERAAALTPDAARHAGRLVAAAEAAWDAGQPERARV
                     LLDAAEPYSTRPRCRARIAGLRGWIEHISGTPTTASVILAEGAAPIMRSDPDLARDLL
                     ILAARSAWLGNEPAWLIEIGERLQQLPGAHDAEFGQLALLIVELGHDRLPRHGSQHQA
                     LAETSTPGQVPEDPRPWIWPPTFLPELLGDELAISERLTKLVAALRARGVIGMLPVAL
                     TQLTPVEVVLGRWNSAVSHASEGIRLAQETEQPTLAAYLRSMLAWLAAARGRADDCRR
                     LVGEACEFAVPHEHVAALTFTSWASGLLALGQGQPDQALARLLPLATPKGPGGGHYLL
                     GRRATGDLVEAAVLAGQAEAAVPVLAEFEAWTRHNQQPWAIHLARRCRGLLSADDAVA
                     ERSFQAALTPDGGGQQRLRPFQFARTELSYGEWLRRGRRRSDARPHLRAALEIFDRLA
                     ATPWAERARAELRASGESARKRDPSTLQQLTPQERQIADRAARGLTNRQIGAELFLSP
                     RTVAYHLHNIFPKLGIASRAELQRHDLTSPPSR"
BASE COUNT         2075 a         5589 c         5928 g         2123 t
ORIGIN      
        1 aggaaggtcc tacctgtcat acaggtcctc gcgtttccat gtgcggccct tggggccact
       61 ggaggcgtgg acggtaccgg cgatattcag aaactcagat attgcctgcc tggtgggatc
      121 tgctccggcg aagctctcca gatagtcacg aacgagcgca ttgacggtcg tatgcagttc
      181 aagagcgcgc atgcgcgccc tctggagcaa ctcgtcatcg acgctgagcg tcagattggc
      241 cacaggccct cctttccgtg tagcacggaa tcagtgtagc acggatgtgg ttcaggaccc
      301 agccagggca gcggcgagct caccggcgag gccggcgttg gcttccagca gggcgaggtt
      361 ggcggcgagg ctgcggccgc cggtgcggcg gtggagccag tcgagttcga agggggtgcg
      421 ggcctttccg acgatgccgg cgtcgcgggc ggcgacggag gaggcggcgg ccagctcggc
      481 cagctcgtcg ggatccatcg ccgcggacgc cggggggttg tggaccagca gcagggcacc
      541 cggggagtcg agcgcccggc ggacccggac cagctcgacg gcttcctcga cgttgttgac
      601 gaggtgctcc aggggaaggt tgccgcgcgc caggaatccg ggcatgcggt cggtgcggta
      661 gccgaccacc aggacgccgt tggtctccag ccattcgagg gtggcggcgc tgtcggcgac
      721 gtgcttggcg cccgagcaga ccaccaccag ccgggaacgg gcgagctgcg ggaggtccgc
      781 ggagacgtcg ttggcgccga agtggacccc gccgatgccg ccggtggcca tcacgtcgat
      841 gccgacccgt tcggccgcga acacggtcgc cgacacggtg gtggcgccgt ccagccgccg
      901 cgccaccaca taggggaggt cccggaccgt caccttccag gtcgtgcggg cggtggcgag
      961 cagctcaacc tggtcggggt cggccccgac cacgatgcgc ccgttgatca ccgcgaccac
     1021 ggccggttcg gcgcccgatt tgctgacggc aagcgactgc gcttcggcgg actcgagact
     1081 ggccgggctc ggcagccctt gggccagcac gctcgattcc agcgccacaa cggcgctggc
     1141 ggtggcgatt cggtcggcaa ccacgatcgg tcggtccatg cgactgcgac gctccctgct
     1201 ctgctcggcg aaacctacgg atgctacgga gagaagctcg tcaagggtaa cggcaacctg
     1261 ggctccgagt catcgccgcg cgacaaccca gcggcggcgc gctgggctcg cgggacgccg
     1321 cgccggtcag cctctcgtgc cggaaacggg atgttcgtcc cagaaccgag ccatcacgac
     1381 gcggcgggca cggcgggtgg cgcggcggaa ggactccagc aggcgctggc ggccgccggg
     1441 cggttcgccc atggtttcgg cgaggcgctc cagcagcagc gggtcggtcg gcagcgagtc
     1501 gaccggccgg ccggcgacca gatagccatg gttgcggagc cgcaggcaga gccggtaggc
     1561 gccggccagc caggcggcct cgcgggcgtc gagcgccctg gcggcggtga gggcgtccag
     1621 tgccgcgaag gtgccctggc ggcggacctc ggggtgggtg ccgccgaggc ggagctggag
     1681 gagctgcacg gtccactcga cgtcggcgag gccgccgggg ccgagcttga ggtgcaggcg
     1741 cgggtcggcc ccggcgggga gccgctcgga ttcgacccgg gccttcatgc ggcggatcgc
     1801 ggcgacctgc tcggcgtcga gccgctccgg gtaggtccag gcagcggccg cctgcccgaa
     1861 ccgcaggccg agtgcgcggt ccccggcgac cggacggacc ttgatgaggg cctggcgctc
     1921 ccagggctcc gcccagcggt cccagtaggc ctggtaggag gcgagcgtgc gggtcagcgg
     1981 cccgttgcgc ccctcggggc ggaggttcgc gtcgaccttg aagccggtcc cctcggcggt
     2041 gaccgcgcca agtccgttga gcagctcgcg ggcgatctgg gcggcggcac gggcagcctg
     2101 ctcggggtcg gcgccgtcgg cgggctcatg gcagagcagg atatccaggt cggagacata
     2161 gttcagctcc tcgccgccga gcttgcccat gcccagcacc gtcacccgca cctgctcggc
     2221 gccacgcacc cggtggctgg cgaccgcgag gcccgcctcg aggctcgcct cggccagggt
     2281 ggccagctcc tgcccgacca cctcgacggc gaccccgccg gccaggtccc gcacggcgat
     2341 ccgcagcagc gcgcgccgct tgaagcggcg cagcccgtcc caggcggcgg ccacctgccg
     2401 gtcgtcgccg tcatggcccg ggccgaggtg gcgggtgacg atcgccaggg cctcccccac
     2461 cagctggtcg tgggtccggc tgccggcggg gccgccggct tcggccatgg cggtgagcag
     2521 ctcggggtgg cgggcgagca ggtcgccgag gaggcgggag ctgccgagca cgatgcacag
     2581 cagctcggcg acggcgggat tgtcgcgcag ggtggcgagg aaggcgccgt ggcggccgag
     2641 cgcctcggcg acgacccgca gggcggcgag gccgctgtcg gggtcggggg cgtcggccag
     2701 ccaggtgagc atggcgggca gcatcgcgcg gaacagggtg gcgcggcgtg acaggccgct
     2761 ggtcagcgac cgcaggtgcg ccagggcccg ctcggggttg gcgaagccga gcgcggcgag
     2821 ccgctcgcgc gcctcccgcg ggtcgagtcc ggtggggacc gcgccgaaga cctccagcag
     2881 cggccggtag aagagcttct cgtggaggct gcggacggtg gccgcgtggc ggcggcggtc
     2941 ggcctcgaac aattccagcg cgtcgccgtc ggggccgtcg cggtagccca tggtgcgggc
     3001 gagccagcgc cgctccccct cgcccgccgg gatggtgtgg gtgcggcgct cgctggcgag
     3061 ctgcaggcgg tgctcgatca tgcgcaggaa gcggtaggcg ctggcgagcg cggcggcgtc
     3121 gctgcggccg acgaacccgg cggcgctgag cgcctccagc gcgaccaggg tcgagccgct
     3181 gcggagttcg gggtcgtggc gggcgtggac cagctggagg agctggacgg cgaactcgac
     3241 gtcacggagg ccgccggggc cgagcttgac ctggcgttcg ccgccgtcgc gggcctgctg
     3301 ggaggcctcg acccgggctt tcatgtggcg gatcgcggcg atcgcctcgg ggtcgagccg
     3361 ctccgggtag gtccacgcgg cggcggcctg gtggaaccgc tcgccgaggg ccgggttccc
     3421 ggcgaccggg cggaccttga tgagggcctg ctgctcccac gcctccgccc agcggtccca
     3481 gtaggcctgg taggagccga gcgtccgggt cagcggcccg ttgcgcccct cgggacggag
     3541 gttggcgtcc acgcggtagc aggtcccttc ggcggtgacc gcgctgagac cgccgatcag
     3601 ctcccgggcg atcgcggcgg cgatccgtgc ggcctcgtcg gcgtcggcct caccctcgtc
     3661 ctccacgccg gaggcagggc ggggggcggg ttcgtggacg aagagcacgt cgatgtcgga
     3721 gacgtagttc agctcctcgc cgccgagctt gcccatgccg agcacggcca cccgcacccg
     3781 ctcggcctcc tcgacccggc ggcccacgac cgccacgacc gccagggcgg cctccaggca
     3841 cgcctcggcg agcgccgcca gctcctgccc gaccacctcg accgccgcgc cgccggccag
     3901 gtcccgcacc gcgatccgca gcagctcccg ccgcttgaag cgccgcagcg cctcccaggc
     3961 gaccaccggg tccgcaaccg gatccgcaac cgggtccgca accgggtccg caaccgggtc
     4021 cggggcggag acggtcgcca gcgcctcctc caccagctcg tcgcgggtac ggggggcggc
     4081 gaacgcgcgc ccgtcggcga ggcggtcgac ctcttcgggg tgggcggcga gccaggcgcc
     4141 gagggagcgg ctggcggcga ccagcgcgac cagccgccgc cggaagccga gctggcggcg
     4201 cagcgccgcg gtgaaacggc cacggtcgcc ctcgggcagg gcagcggcga ccgccccgag
     4261 cgcccgtgtg gccagttcgg ggttggcgct cccgcccaat tcgtggagca ggcgcaggcc
     4321 gcccgcggcc gcatgcggcg gccaggcccc cagcgcctcc aggtcgccga cgaccgccag
     4381 cggctcctcg aagccgagcc gtgccaggaa tgggccgggc tcaggcgcag ccgaacccct
     4441 tggcctgctg tgcggcaccg ccactattgc ccccagatgt ccgccctgac aagcgtcggg
     4501 cctaccaaca ggcacgccaa aacccgcgcg agggggtccc cctgtttaac gctatcggat
     4561 ttggcccgga tggcgtcgcg ttgtccacag ccctccgcca gcggcgctca ggtggtccag
     4621 cgccaggccc ggacggcccg gtcgagggcg gcgacgtcgc cggggaaggc ctgcgtcgag
     4681 gcggagaagg tgagccacca gaccgcgccg tggtggtagc aggcgatctg gcgcacccgc
     4741 actgggtggc cgccctgggc cagcgtccag tcggtggcga tcgccgccgc actgtcgagc
     4801 tggagcggcc aggcgctgcc gaccgggacc gcgccgaggg tgtcgcgcag gcggcccggc
     4861 agcgccgcgg cgagccgcgg cagcgacggc ggctgtgcgg ccggctgccg gagcacgctc
     4921 aggtcggcca caaatccgct ggtcgcgcgg cctgtcagga cctggactgg ccgcacgccg
     4981 cggtaggagc cggcgagctg gcgggtctgg tcacgccacc cggacggcag cggaaaggca
     5041 tactcgcgcc cggcacccgc acccgccgcg acgacccgca ccggacggcc gagcgccggg
     5101 tgggccacat gggccgggtg gaaggtccgc gtgaccggag cccgcgcgac caccatcagg
     5161 gccacgacca gcgcgaccac gcccaccagg gtgaccagcg ggctgcggcg ccgccgtgac
     5221 ggggacggcg gacgcgccgc ccgccccgat gccggggacg cctcgggcgc cctccaggag
     5281 gaatctggtt cgtcgtccca gcctgaggag tcgtcccagc ccgaattcgc ccccatgtgt
     5341 ttgcctgccc cctcgttggg tcttcagctg cgacggatgt ggcgcccggg tcagagcagc
     5401 ggcaggtagc gcttgcgctc gaacggggtg acgtagcgct ggtaggcctg ccagtcgcgg
     5461 cgcttgttgc gcaggaagta ctcgaagacg tgctcgccga gcgcctcggc gaccagctcc
     5521 gagctctcca tggccaccag tgcctcgtgc aggtcgactg ggagcggttc gatgccggcg
     5581 gcgcggcgct ccgcctcggt catccggtcg agggcgtcgt cggcctcggg cggcagctcg
     5641 tagtcctcct cgatgccctt catgccggcg gcgagcagca ccgcgaacgc gaggtagggg
     5701 ttggcggcgg ggtcgggcga gcggacctcg acgtccaccc ggtcggagcg gtagccgccg
     5761 acccgcacca tcgcggagcg gctgcgctgc gcccaggcga tgtggaccgg cgcgtcgaac
     5821 ccgggcacca gccgcttgta ggagttgacc cactggttgg tgaccgcggt gtactcccgc
     5881 gcgtggacca gcagccccgc gatgaacgag cgggcggtct tggagaggtt ggcctcctgc
     5941 ccggggtcgt agaaggcgtt gcggtcgccc tcgtagaggc tgaggtgggt gtccatgccg
     6001 gagccgtcca cttcctggat cggtttcggc atgaagctgg catgcaggtc gcgttccagc
     6061 gccgcctcct tgaccaccag gcggcagctc atcacgttgt cggccatggt gagcgcgtcg
     6121 gcctcgcgca ggtcgatctc gtgctggctg ggagcgatct cgtggtggga ttgcatcacc
     6181 gagatgccca tccgttccag catgtcgatc gcgtcctggc ggaagtcgtg ggcgagatcc
     6241 agcgcggtct gctcgaagaa cgacccggag tccagcggcc gcaccgattt cgcgttcttg
     6301 aagaggaaga actccagctc cggctgcacg tagacggtga agccccggtc ggcggcgcgt
     6361 tgcaggttgc ggcgcagtac ccagcggggg tcggcgtcga acgactcacc ctcagtggtc
     6421 agcacgtcgc agaacatccg tgcgacgccg ccgtcgccgc cggtgcggcc cggcagcacc
     6481 tggaaggtgc tcgggtcggg gcgggcgacc acgtcggcct cctgcgcgcg ggcgtagccc
     6541 tcgatcgcgg agccgtcgaa ggtcatgccc tgctcgaacg cctcctccag ctcggccggg
     6601 gtgatcgcga acgacttgag gaagccgagc acgtcggtga accacagccg gacgaagcgg
     6661 atgtcgcgct cctccaccat ccgcaggacg tattcgcgct gcttgtccat gtcgtagacg
     6721 ctccggatcg acggtcgggc gccaatgcta ccgctggtac cgtggattcc ggagcaccgt
     6781 ccacaaccgt ttacaagctt gttcgggagg tgccggatgg agcgcctgcg gctcgccgga
     6841 gcgcagctca accaggtcgt cggggacatc gacggcaatg tggagcggat cctggcaatc
     6901 taccgcgaag ccgccgcgaa gggtgcgcac ctggcggtgt tcccggagct ggcggtcacc
     6961 ggctacatgc ccgaggacct ggtcttcaag cccgacttcc tggcagccag ccaggccggg
     7021 atcgagcggg tcgccgccgg ggtgggcgcc tgcgccgcgg tggtcggctt tgtcgaggcg
     7081 accccggccg ggccggcgaa cgccgccgcg ctctgccacg acgggcggat cctggcgacc
     7141 taccacaagg tgctgctgcc caactacagc gtcttcgatg aggagcgcta cttcatcccc
     7201 ggccgcgagg tcctgctcgc cagcttcggc gaggtcagcg tcgccatcac catctgcgag
     7261 gacctgtggt tcccctgggg cccgatggcc gccgcggcgg cggccggggc cgaggtggtg
     7321 gtgagcatca acggctcccc ctaccgcctc ggcaaggccg accgggtcgg cccgatgctg
     7381 gccacccgcg ccgccgatca tggcgcccac ctcttctacg tgaaccgggt cggcggccag
     7441 gacgaactgg cctacgaagg cgagagctgc tacttcgacc cggagggccg cgtccgcgcc
     7501 cgggccgcca gcttcgagga ggacctgctg ctggtcgacc tgaccctgga ggagggcgcc
     7561 accgcccgcc tcgccgaccg gccacggcgc acccccgcca gcgtcccgcc cggggccggg
     7621 tcgcccccga gcctcgctgt ccggcgcgtc caggtcaccg accgcctgcc tgacccgggc
     7681 ccgctggtcg agcggcgcct gtccccgcgt ctgggcgccg aggaggaggt ctaccgggcg
     7741 ctagtctgcg cgacccggga ctatgtgacc aagaacggct tcggcgcggt ccacgtcggc
     7801 ctgtcagggg ggatcgactc cagcctggtg gccgccatcg cggtcgacgc gctcgggcct
     7861 gaccgggtca ccggggtggc gatgccgtcg tcgtactcgt cggaggggag cctccgcgac
     7921 gcccaggcgc tcgccgccaa tctcgggatc gagctgctca ccctgccgat ccacgacccg
     7981 gtcgacggct tcgccgctgc cctgaaggag ccgttcgccg gcaccgagcc gggcctggcg
     8041 gaggagaaca tccaggcgcg ggtgcgcggc acgctgctga tgagcctctc gaacaagttc
     8101 ggcaccatgg tgctgaccac cggcaacaag tccgagatgg cggtcgggta ctgcaccctg
     8161 tacggcgaca tggccggcgg cttcgcggtc atccgcgacg tccccaagac gctcgtctac
     8221 gccctggccc gctggcgcaa ccagcaggcc cacgcgcgcg gagcgggcga ggtgatcccc
     8281 cagtcggtgc tggacaagcc cccgtcggcg gagctgcgcc ccggccagct cgacaccgac
     8341 tccctgccgc cctacgaggt cctcgacccg atcctggaac gctacatcga ggacgacgcc
     8401 tcgactgacg agctggtcgc cgaaggcttc gaccgcgccg ttgtcgaacg ggtcgtcggc
     8461 ctggtcgacg gcgccgaata caagcgccgc caggccccgc ccggccccaa gatcaccggc
     8521 cgctccttcg gcaaggaccg ccgcctgccg atcagcaacc gcttccgcgg ctacccgcgc
     8581 gccgccaggt agccggccgc caggcgggca gccggggcca aattcgctgg ctgcgaccgg
     8641 gctcggaacg gcacgatctc cactatgggt tcgggttcgg agggcggggg cgtggagggg
     8701 ctcgccggcg ggtcgcgctg gcggcgctgg cggcgactcg cggtggtcga caacaggccg
     8761 ctgcgggagc ggcgggactt ccggttgctg ttcatggcgc agggagggtc gacgtttggg
     8821 acccggatca cctatgtggc catcccctat caggtgttcc ggatcacgcg ttcgccgctg
     8881 gcggtggggc tgctcgggct ggcggagctg acgccgctgc tgctgacggc gctgctcggc
     8941 ggggcgctcg ccgacgcgca cgaccggcgg cggatggtgc ggctcaccga gctgggcatg
     9001 gcgacggcgt cggcgtcgct gctggtcaac gcggcgctcc cccaccccgc cctttgggtg
     9061 ctgtatgcgg cgtcggtggt gctggcggcc ttggacgggc tgcagacgcc gtcactggac
     9121 gcgctggtcc cccggctggt cgaacgcgac gaactgccgg ccgcggtcgc gctggaaggg
     9181 ctgcgcggca ccctggggat ggtggccggg ccggcgctcg gcggggtgct ggtggcgacg
     9241 gcgggcctgc cggtcgccta cggggtggac gtggcgacct tcggattctc gctgctgctg
     9301 ctgtcgcgca tgcgggccgt gccgccaccg ccgggggccg cgcgggtgag cctgcgcggc
     9361 atcctcgacg ggctgcgcta cgcgcgcagc cggccggagc tgctcggcac ctatgtggtg
     9421 gacatcgtgg cgatactgtt cggcatgccg ctggcgctgt tcccggcgat cgccgaccgc
     9481 tacggcggcg ctggggtgct cggcctgctg tacgccgcgc cctcggccgg ggcgctgctg
     9541 gccaacctca ccagcggctg gctcgggcgc tcacggcggc acggcgccgg catcctgggc
     9601 gccgccggct gctgggggct cgccatcgcc gccttcgggg tggcctatgg cctgggtggg
     9661 ccgctgtggc tggcgttgct gctgctcgcc gcctccgggg gtgccgacat ggtgagcgga
     9721 ctgttccgct ccaccatctg gaatcagacg atcccggacg agctgcgcgg ccgcctcggc
     9781 ggcatcgagc tgctcagcta ctcgatcggc ccgacgctcg gcaacgtcga ggcgggcgcg
     9841 gtcgcgtccg ccttcaccgt gaccacctcg gtggtctccg gcggggcgct ctgcgttgcc
     9901 ggcgtgggcg tctgcgccct gctgctgccg gccttccgcc gctacgacgc cgcgaggttt
     9961 ctggccgcgg cgcgggcaaa cgccgcgtag gcgcccgttt cggcggctgg cgggtgcaca
    10021 ctggaggagc caggagaccc ccatccgggg ctccaaggaa ctccagaagg aggccgcgat
    10081 gccggtcacc atcaccgatc tcgccgcgtg gaaggagcgc ggggagcggt ttgtcatgct
    10141 caccgcctac gacgcgctca ccgcgcgggt gctgggcgag gcgggagtcc cgttgctgct
    10201 ggtcggcgac tcgctgggga acaacgttct cggctacgac tcgacgatcc cggtcaccat
    10261 ggaggagtcg ctggtgttca cggcggcggt ggcccggggc gcgaggaacg cgatggtcgt
    10321 cggggacctc cccttcgggt cataccaggc cgcggccgcc gacgccatgc gcaacgccgc
    10381 ccgcctggtc aaggtgggcg ccaacgcggt caagctggag ggcgggcagc gctcggtcga
    10441 ggccgtcagg ctgatcgtcg cggccggggt gccggtcatg ggccatctcg gcctcacgcc
    10501 gcagtcggcc aacagtttcg gcggcttccg ggtgcagggg cgcggcgagg cgggcgaccg
    10561 gctgctggcc gacgcgctct ccctgcaggc ggctggctgc ttctcgatcg tgctggaggc
    10621 gatccccgcc gagctgggca agcgggtcac cgaggccctc cagatcccga ccatcggcat
    10681 cggcgccggg ccgcacaccg acgcgcaggt gctggtcatc accgacctgc tcggcctcac
    10741 ccccgacccg gcgccgcggt tcgtgaagcg ttacgccgac ctgcgctcca ccatcgccga
    10801 ggcggcccag cgcttcacca aggacgtcgc cgagggccac tatccgggac cggaacacca
    10861 gtaccactga gcgacggagc gccacggcgc tgtggttgcg tttgcaacca cctctggagt
    10921 agttgtagat tcaactatgc cttcccactg ccgagaggag ccctagatga gcgaagcacc
    10981 cacggtccca agcggatccc gggtggtcga cggcctggtc gtcccagagg ccggcacctg
    11041 ggtcgcggat gcggcccact ccaccttcga gttcgtcgcc cggcacctga ccgccaaggt
    11101 ccgcggccgc ttccacaccg ccaccgtcac cgcccacgtc gccgaggtcc cggagcagtc
    11161 aacggtcgag gtcgagatcg acacggccag cgtcgacacc cacgagccgc gccgcgacgg
    11221 ccacctcaag tcgccggact tcttcaacgt cgagaagtac ccgaagatga ccttccgctc
    11281 caccgggctg cgctcagcgg gcgagggcgt ctgggagctg acgggcgacc tgaccatgct
    11341 cgacgcgacc cgcccggtca gcttcgacct cgaattcctc ggcaccagcc ctgatccgtg
    11401 gggcggcatc cgcgccggtt tctccgcctt cgccgaggtc gaccgcgagg actggggcct
    11461 caactggaac tcggccctgg agaccggcgg ctggctgctc tccaagaaga tccgcctcga
    11521 gatcgaggtc gaagccgtca ggcagtaggc ggtcgacggg ctggggggcg gcgccgacgg
    11581 cagccggggg ggaccctggc catcggcgcc gcagcccagt cggcggcaac cggcacctgg
    11641 gggaggcgca gcggtcgctg gcgacctgac aaccttgcca cagcacggct gagacgaagc
    11701 tgaacgctcc tgagaggacg ctgagaggac cccaggaggg ccggggagcc gcggctcccc
    11761 gccggatatc cgcccagctc aggccgttcc gttggctcgt gaccctcggc gtgaccatcg
    11821 ctggggccgt cggcaggcag gcgcaccacc agcctcgcgc cggaaccgcc cgaatcattc
    11881 ggatcgttcg aaccctggcc ggcgacggcg acggcgacgg tgccgccgtg cgcctggacg
    11941 atctcccgga cgatcgccag ccccagccct gccccgccac ggtcggggct gcgggcctcg
    12001 tccaggcggg tgaaacgctc gaagacccgc tcgcggtcct ccggcgggat gccagggccg
    12061 tcgtcagcga cgaccagctc ggcctcgccg ccgtcgagcc ggcgcacctc cagccgcacc
    12121 tgttggcggg cgtgacgctc ggcgttggcg gccaggttgc gggcgacctg ggtgaggcga
    12181 tggcggtcgc cggtgacgcg ggcggccgag atcccggagg tgtccaccat gaccttgcca
    12241 cggttgcgca gccgccgtgc ctccgccaac gccagctcgt caagatcgac ctggtcgcgc
    12301 cgcagcggca aggtcgcctc gtcgacccgg gcgagcagca gcaggtcctc gacgatccgc
    12361 tgcatccgcc gcacctcctg catgaccgcg tccgcgaccg ccctccagtc ggtgcgctca
    12421 ggaagcgcca gcgcgacgtc gagctgcagc tggatcgcgg tgagggggct gcgcagctcg
    12481 tgcgaggcgt cagccacgaa gcgccgctgg cgcgccgctg acgcctccag ctgttccagc
    12541 agctcgttca tggaccggac cagcgggacg acctcgccgg ccgcccgctg gtcagcgatc
    12601 cgcagggaga ggtcccgccg ggcgatctcg ccggcctcga cccggatcgc ctccaccggc
    12661 cgcagggcgt gggcggccag gaaccaggcg gcgaccgcca ccagcaggat cagcaggggc
    12721 gtctgcaggt cgagcccggt ctgggtggtg tgctccgaca gctcggcgag gtggagggac
    12781 gccgccacaa agacgatgcg cgccccctcc ctcggggtgg tgacgcgggt tgagagcatc
    12841 aggtaggggc cgcgcccgtc cgcgacgaca gcgggcagcc ggcgcaggtg ggcgacgctg
    12901 acggtgtcgc cccgcagcgg ctgcccggcg gccgccagcg ccggggcgcc gtgcagcgcg
    12961 gggctctcca gcgacacaag accggcggtg tcgaccacct ggatcaggtc gcctggcgcg
    13021 ccatggagca gctcctcggc gggggcaccg gtccgcagca gccgggcgac ccgcaacacg
    13081 tcggtccgcg ccgtcgactg gatgctcgcc tccagtttgt ggtgctgcat ccgcaggagg
    13141 gcgaagtcgg ccggcagcaa cgcgatcgtc acggcgaccg ccgccaggac ggccgcgcgt
    13201 gcccgccgcg accgcaggac cgcgggcagt ctagccaccg tcggcggcga gccggtagcc
    13261 gaccccgcgg accgtctcga tcgcgttgcg gccgaagggg acgtcgatct tgcgccgcag
    13321 gtagccgatg tagacctcga cgatgttcgg gtcgccgtcg aattcgaggt cccagacctc
    13381 gtcgaggatc tggcgcttgg cgagcacctc gccggggtgt cgcatgagca gctccagcag
    13441 ggcgaactcg cgggccgtca gcgagatctc cgtcccactc cggtcgaggt ggtgcctggc
    13501 cgggtcgagg cgcaggtcgc cggcgctgag caccgtcggc cgctcgcgcg ggccgcgccg
    13561 cagcagcgcc cgcagccgcg ccaccagcac cacgaaggag aacggcttgg tgacatagtc
    13621 gtcggcgccg gtgtcgagcg cctccgcctc gtccaggtcg ccgtccttgg cggtgagcat
    13681 cagcaccggc gtccagatct cggcgtcgcg cagggccgcg cagacccggt agccgttcag
    13741 gccgggcagc atgatgtcga gcacgatcgc gtcgaacggg tgctcctggg cgcgccagag
    13801 cccgtcgttg ccgtcatggg cgacctcgac gacgaagccc tcggcctcca gcccgcggcg
    13861 aagcgtctcc gcgagcgggc gctcgtcctc caccaccaga actcgcatca ggtcctcatc
    13921 gggatggcgg gctggtcagg tcgtggcgtt gcagctcagc ccgggaggcg atgccgagct
    13981 tggggaagat gttgtggagg tggtacgcga cggtccgcgg gctcaggaac agctccgcgc
    14041 cgatctgccg gttggtcagc cctcgcgcgg cgcggtcggc gatctggcgc tcctgcgggg
    14101 tgagctgctg gagggtgctc gggtcacgct tgcgggcgct ctccccgctg gcccgcagct
    14161 cggcgcgggc acgctcggcc cagggggtcg ccgccagccg gtcgaagatc tccagggcgg
    14221 cccgcaggtg cgggcgggcg tcgctgcggc gccggccgcg gcgcagccac tcgccgtagc
    14281 tcagctcggt ccgggcgaac tggaagggcc gcaggcgctg ctggccgccg ccgtcagggg
    14341 tcagcgcggc ctggaagctg cgctcggcga cggcgtcgtc ggcgctcagc aggccgcggc
    14401 agcgccgcgc cagatggatc gcccatggct gctggttgtg cctggtccac gcctcgaact
    14461 cggccaagac gggcacggcc gcctcggcct ggccggcgag caccgccgcc tccaccaggt
    14521 cgccggtggc gcggcggccg agcaggtaat ggccgccgcc ggggcccttg ggggtcgcca
    14581 gcggcaggag gcgtgccagg gcctggtcgg gctgtccctg gcccagcgcg agcaggccgg
    14641 acgcccagga agtgaacgtc agggcggcga cgtgctcgtg cgggacggcg aactcgcacg
    14701 cctcgccgac gaggcgccgg cagtcgtcgg cgcggccccg cgcggcggcg agccaggcaa
    14761 gcatggaccg caggtatgct gccagggtcg gctgctccgt ctcctgggcg agacggatcc
    14821 cttcgctcgc gtggctgacc gcgctgttcc agcggcccag cacaacctcg accggggtca
    14881 actgggtcag cgcgaccgga agcatgccga tcaccccccg agcccgcagg gccgcgacca
    14941 gcttggtgag ccgttcgctg atcgccagct cgtcgccgag aagctcggga aggaacgtcg
    15001 gcggccagat ccagggtcgc gggtcctcgg gcacctgccc gggcgtggac gtctcggcca
    15061 gggcctggtg ctgcgagccg tgcctgggga ggcggtcgtg gccgagttcg acgatgagca
    15121 gggcgagctg cccgaactcg gcatcgtggg cgccggggag ctgctggagg cgttcgccga
    15181 tctcgatcag ccatgccggc tcgttgccga gccacgccga gcgcgcggcc aggatgagca
    15241 ggtctctggc caggtcgggg tcgctccgca tgatcggagc ggcaccctcg gccaggatca
    15301 ctgacgcggt ggtcggcgtc ccgctgatgt gctcgatcca gccgcggagg cccgcgatcc
    15361 gggcacggca cctcgggcgg gtgctgtagg gctcggcggc gtccagcagc acccgggccc
    15421 gttcgggctg gccggcgtcc caggcggcct cggcggccgc gaccagccgt ccggcgtggc
    15481 gcgcggcgtc gggggtgagg gcggcggcac gctcaagggc gtccgcggcc gccacggtgc
    15541 cgccccgccg ggcggcgcgg tcggcggtgc gctccagcgc cgaggcgacc tgctcgtccg
    15601 ggccggtcgc ggcggcggcg aggtgccagg cacgctggtc ggcgcggtgt tccccggcca
    15661 ggacctgcgc gaggcaggtg tgggcggcca ggcggtcggc gatcggggcg ctttg
//
LOCUS       DASWDC010000297         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_125084, whole genome shotgun sequence.
ACCESSION   DASWDC010000297 DASWDC010000000
VERSION     DASWDC010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125084"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2319
                     /locus_tag="VF995_09625"
     CDS             <1..>2319
                     /locus_tag="VF995_09625"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX9343858.1"
                     /translation="EAFVERVWEWKAASGGRILGQMQRLGDSVDWSRERFTMDEGLSR
                     AVRHAFVSLYEQGLLYRGQRIINWCPRCLTALSDIEVEHEEVPGELAHLRYPLADGSG
                     HIEVATSRAETMLGDTAVAVHPEDPRYRGLIGRTVRLPLVDREIPVVADEAVDRAFGT
                     GAVKVTPAHDPNDHEIAQRHDLPAINVLTEEAIVNHNGGRFQGLDRYAAREAVKRAFA
                     DLGLLGKVVAAPHAVGHCYRCTTEVEPRLSLQWFVATKPLAEEAMAAVRGGRTRFEPA
                     RYEKTFFGWMESIRDWCVSRQLWWGHRIPAWYCPDGHLTVARDDPDTCAECGQGGLTQ
                     DEDVLDTWFSSGLWPLSTLGWPDQTEDLAAFYPTSVLVTGYDIIFFWVARMLMLCCHF
                     KPQTPFDVVAIHGMVRDAHGKKMSKSFGNVIDPIELMDRYGTDALRFALIRGANPGSD
                     VPLAEEWVEGARNFANKLWNLGRFTLSSLTTTRGEGVAGDTPASPAVAPGGEALADRW
                     LASRLERTRAAVSAAYDHYDPAEAARLLHAFAWSELADWAVELAKPRLAAGGEDARRA
                     GATLVGAFDVTLKLLHPIMPFVTEELARALSGVETITLGPWPQERPGDLDADAEAAMA
                     DLQEVIAALRRFRAEHHVPTASKPRCVLVPADARQAALFEAEAERVRRLTRLAELTVA
                     PPGTAPSGEPATKLLAAGVELYLPLAGLLDLDEERRRLEREQATLEAERDRATAKLAN
                     PDFVAKAPPPVVDKARARLAEVEADLVKVTEQL"
BASE COUNT          321 a          922 c          767 g          309 t
ORIGIN      
        1 cgaggcgttc gtcgagcggg tgtgggagtg gaaggccgcc tccggcgggc gcatcctcgg
       61 ccagatgcag cgcctcggcg actcggtcga ctggtcccgc gagcgcttca ccatggacga
      121 gggactctcc cgcgccgtcc gccacgcctt cgtcagcctc tacgagcagg gcctgctcta
      181 ccgtggccag cgcatcatca actggtgccc ccgttgcctc accgccctct ccgacatcga
      241 ggtggagcat gaggaggtcc ccggcgagct ggcccacctg cgttacccgc tcgccgacgg
      301 ctccggccac atcgaggtgg cgacctcccg cgccgagacg atgctcggcg acaccgctgt
      361 ggccgtccac ccggaggacc cgcgctaccg cggcctgatc ggccgcaccg tgcgcctccc
      421 gctggtcgat cgcgagatcc cggtcgtcgc cgacgaggcc gtcgaccgcg ccttcggcac
      481 cggcgcggtc aaggtcaccc cggcgcacga ccccaacgac cacgagatcg cgcagcgcca
      541 cgacctcccc gcgatcaacg tgctcaccga ggaggcgatc gtcaaccaca acggcggccg
      601 cttccagggc ctggaccgct acgccgcccg cgaggcggtc aagcgggcgt tcgccgacct
      661 cggcctgctc ggcaaggtgg tcgccgcgcc ccacgcggtc ggccactgct accgctgcac
      721 gaccgaggtc gagccgcgcc tgtcgctgca atggttcgtg gccaccaagc cgctcgccga
      781 ggaggcgatg gcggcggtcc gcggcggccg cacccgcttt gagccggccc gctacgagaa
      841 gaccttcttc ggctggatgg agagcatccg cgactggtgc gtctcccggc agctgtggtg
      901 gggccaccgc atccccgcct ggtattgccc cgacgggcat ctcaccgtcg cccgcgacga
      961 ccccgacacc tgcgccgaat gcggccaggg cggcctgacc caggacgagg acgtgctcga
     1021 cacctggttc tccagcgggc tgtggccgct ctcgaccctt ggctggcccg atcagaccga
     1081 ggacctggcc gcgttctacc cgacctcggt gctggtcacc ggctacgaca tcatcttctt
     1141 ctgggtcgcg cggatgctga tgctctgctg ccacttcaag ccccagaccc ccttcgacgt
     1201 ggtcgccatc cacgggatgg tccgcgacgc gcatggcaag aagatgtcga agtccttcgg
     1261 caacgtcatc gacccgatcg agctgatgga ccgctacggc accgacgcgc tccgcttcgc
     1321 gctgatccgc ggcgccaacc ccggcagcga cgttccgctg gccgaggaat gggtcgaggg
     1381 cgcccgcaac ttcgccaaca agctctggaa cctcggccgc ttcacgctgt cctcgctgac
     1441 cacgaccagg ggcgagggcg tggcggggga cactcctgcc agccccgcgg tcgcccccgg
     1501 cggggaggcg cttgccgacc gctggctcgc ctcgcgcctg gagcgcaccc gcgcggcggt
     1561 gagcgccgcc tacgaccact acgacccggc ggaggccgcc aggctgctgc acgccttcgc
     1621 ctggagcgag ctggccgact gggccgtcga gctggccaag ccgcgcctgg ccgccggtgg
     1681 ggaggacgcc cgccgggccg gcgccacctt ggtgggcgcg ttcgacgtga ccctcaagct
     1741 gctccacccg atcatgccgt tcgtcaccga ggagttggcc cgcgccctct ccggcgtgga
     1801 gacgatcacc ctcggcccct ggccacagga gcggcccggc gacctcgacg ccgacgccga
     1861 ggcggccatg gccgacctgc aggaggtcat cgccgcgctg cgccgcttcc gcgccgagca
     1921 tcacgtcccc acggccagca agccgcgctg cgtgctcgtc cccgccgacg cccgccaggc
     1981 cgccctgttc gaggccgagg ccgagcgcgt ccggcgcctc acccggctcg ccgagctcac
     2041 cgtcgcccct cccggcacgg ccccgagtgg ggagccggcc accaagctgc tggccgccgg
     2101 cgtggagctg tacctcccgc tcgccggcct cctcgacctg gacgaggaac gccgccgcct
     2161 ggagcgcgag caggccaccc tggaagccga gcgcgaccgc gccaccgcca agctcgccaa
     2221 ccccgacttc gtcgccaagg cacccccgcc ggtggtcgac aaggcccgcg cccgcctcgc
     2281 ggaggtcgaa gccgacctgg tcaaggtcac cgagcagct
//
LOCUS       DASWDC010000298         5804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_961058, whole genome shotgun sequence.
ACCESSION   DASWDC010000298 DASWDC010000000
VERSION     DASWDC010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961058"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VF995_09630"
     CDS             <1..406
                     /locus_tag="VF995_09630"
                     /inference="COORDINATES: protein motif:HMM:NF021201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2399 domain-containing protein"
                     /protein_id="HEX9343859.1"
                     /translation="AEATLGDGAGPLVCVEGWMNGAAAKLLDALAGAGAQLAYHGDFD
                     WPGIRIAANVLRRFPAVPWRFHAADYLAAAAARPAGLPRLEGRPAATPWDPALAEAMA
                     TTGLVVEEEAVLADLLADLALAAGGARGGQGR"
     gene            complement(394..2166)
                     /locus_tag="VF995_09635"
     CDS             complement(394..2166)
                     /locus_tag="VF995_09635"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX9343860.1"
                     /translation="MLRRTILALLVTAAAWLLPLVTGAVAVADAHAELRGSTPAGGAA
                     LTRAPQQVVITFTEEADPTLSTIHVFDAHGRQLSGQTMAVMGAPLQLRAVIVAAQLAK
                     APGAYTVRWRSVSKDDGHVASGAFTYTLSAGGGTASGAPAARRAPAATAAPAPSSASG
                     SSGWSSSQPLLGVAGRWALYWALALLVGAASTGLFVLGDARRRRLPVEGGLLLAPLLL
                     GILGLVAMIVAEHAAVGSAVSLQALMHAGAGRDTARVALAFAPAAIATVLLAADPGSP
                     RRLWLLGGSAALAMLVLAVGGHAAAPSSLRPLNLLAQWAHLVAVGVWIGGLAWLLAAL
                     YRQDRAVQIVAAVRFSRVATLALAAVAVTGLERALSELGSWHALFGSSFGRMLDLKVL
                     LFGVLVAFGTYHHFRVVPTLPHDPGGLALLRRGLRGELGVAGLVLLATASLSQLPPGS
                     AASTPMAMTTASHAPATPSIGATGRDATASVRVALQVTPGHPGANVFAAKVINEATGT
                     LLPVRAVQLRLALPARPDRGASTLDLAPGATGVWHARSGALSLDGRWTITALITTATG
                     PVAVALQLRTTPAPPQAQAVGYRP"
     gene            2380..3915
                     /locus_tag="VF995_09640"
     CDS             2380..3915
                     /locus_tag="VF995_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX9343861.1"
                     /translation="MEHDFDTLYRAAQSRDPRFDGRFYTAVTSTGIYCRPVCPAQTPR
                     PENVRFYQCAAAAEAAGFRACRRCRPDTAPGSAEWNVRGDLAARALRLIAAGVVDGEG
                     VDGLASRLATSPRHLHRQLVAEVGAGPLALARTRRAQTARLLIDQTDLPVTDVAFSAG
                     FASIRQFNDTIRAAFGTTPTELRRRRRPLLDSTGGMVLRLRHRTPFDADGLLGYLGQR
                     ALPGVEEAAGGTFRRVLVLPRSTAVAELDLVDNGAEVRLRLRLDDLRDLSVAVQRCRW
                     LLDLDADPAAIGEVLGGDPLLAPLVAARPGLRVPGAVAGLELAVRAILGQQISVAGAR
                     TLAGRLVNALGEPLPQADGSLTHRFPTAEALATLDPATPQPGLGLTRGRLVALRGLGQ
                     AVAAGELDLDRGADREQTEKTLLALPGIGPWTAAYIAMRALGDPDALPAADLGLRRAL
                     ERHGLPGDPASIRARAERWRPWRAYAVLHLWAGTAGTAGPPRPPQEPAQEPAPETEKE
                     PVA"
     gene            3912..4439
                     /locus_tag="VF995_09645"
     CDS             3912..4439
                     /locus_tag="VF995_09645"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX9343862.1"
                     /translation="MNLTTATTETPIGPFTVITGDGDDGEGPVVLAAGFTDQPEELRV
                     RLHKDWRKLPLARADRLGEVSDALDAYFAGDLKALDGLPVKQVNNDRMLRAWAGLRTI
                     PAGETISYRDFGVTVFGDPSYARVAGGACARNLVALIVPCHRVIRTDGNLGGYYYGLD
                     RKRWLLQHERTQDQA"
     gene            4558..4893
                     /gene="cutA"
                     /locus_tag="VF995_09650"
     CDS             4558..4893
                     /gene="cutA"
                     /locus_tag="VF995_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX9343863.1"
                     /translation="MADTYVQVTTTTDSRDDAKWLARDVVERRLAACAQVLGPIDSVY
                     WWEGAVEQAAEWLCLMKTTTGRLDALIAHLRSGHSYDNPEITATPISAGSADYLTWIS
                     QETGTAAGS"
     gene            4979..>5804
                     /locus_tag="VF995_09655"
     CDS             4979..>5804
                     /locus_tag="VF995_09655"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9343864.1"
                     /translation="MTDHSALHLQQSDPEIAQLVAAEERRQAEKIRLIPSENYVSRAV
                     LEATGTVLTNKYSEGYPGKRYYEGQQIVDQVETLAIERAKALFGVDHANVQPYSGSPA
                     NFAVYFAFLEPGDTVMGMALPMGGHLTHGWNVSVTGKWFRSVQYGVRKDTGRVDLDEV
                     RELALKERPKLLFCGGTAIPRTIDFAGFAAIAKEAGALLVADIAHIAGLIAGGAHPTP
                     AGHAPVISTTTHKTLRGPRGAMLMSDAEHAKALDRSVFPGLQGGPHNHTTAAIAVAL"
BASE COUNT          821 a         2201 c         2117 g          665 t
ORIGIN      
        1 cgccgaggcg accctcggcg acggcgccgg cccgctggtc tgcgtcgagg gttggatgaa
       61 cggcgccgcc gctaagctcc tcgacgccct cgccggggcc ggtgcccagc tcgcctacca
      121 cggcgacttc gactggccgg gcatccggat cgccgcgaac gtcttgcgcc gcttccccgc
      181 cgtcccgtgg cgcttccacg ccgccgacta cctcgcggcg gccgcggccc gccccgccgg
      241 gctcccgcgg ctcgaaggcc gcccggcggc gaccccttgg gacccggcgc tcgccgaggc
      301 gatggccacg accggtctcg tcgtcgagga agaggccgtc ctcgccgacc tgctcgccga
      361 cctcgccctc gcagcgggcg gcgcgcgagg cggtcagggg cggtagccca ccgcctgagc
      421 ctggggtggg gctggggtgg tgcggagctg gagggcgacc gcgacgggcc cggtggccgt
      481 ggtgatcagg gcggtgatgg tccagcggcc gtcgagggag agggcgccgc tcctggcgtg
      541 ccagacgccg gtggcgccgg gagcgaggtc gagggtggag gcgccacgat cggggcgggc
      601 aggcagggcc aggcggagct ggacggcgcg gacggggagc agcgtgccgg tggcctcgtt
      661 gatgaccttg gcggcgaaga cgttggcgcc gggatggccg ggggtgacct ggagggcgac
      721 ccggaccgag gcggtggcgt cgcggccggt cgccccgatc gagggcgtgg ccggggcgtg
      781 gctggcggtg gtcatcgcca tcggggtgga ggccgccgag cccgggggga gctggctgag
      841 ggaggcggtg gcgagcagca ccagcccggc gacacccagc tcgccacgga ggccgcggcg
      901 gagcagcgcg aggccgccgg ggtcgtgcgg gagggtgggg accacgcgga agtggtgata
      961 ggtgccgaag gcaaccagga cgccgaacag caggaccttg aggtcgagca tgcgcccgaa
     1021 gctggacccg aagagcgcgt gccagctgcc cagctcagag agggcgcgct ccaggccggt
     1081 gacggcgacc gccgccagcg ccagcgtggc cacacgggag aagcgcacgg cggcgacgat
     1141 ctggacggcg cggtcctggc ggtagagggc ggcgagcagc caggccagcc cgccgatcca
     1201 gaccccgacc gcgaccaggt gggcccactg ggccagcagg ttgagggggc gcagcgacga
     1261 cggcgccgcc gcgtgcccgc ccaccgccag caccagcatg gccagggcgg cgctcccgcc
     1321 gagaagccag aggcgccggg ggctgcccgg gtcggcggcc agcagcacgg tggcgatcgc
     1381 cgcgggagcg aacgccagcg ccacccgggc ggtgtcgcgc cccgcgcccg cgtgcatcag
     1441 cgcctgcagc gagaccgcgc tcccaacggc cgcgtgctcg gcgacgatca tggcgaccag
     1501 gccgaggatg ccgagcagca gcggcgccag cagcagcccg ccttcgaccg ggaggcggcg
     1561 acgccgcgcg tcgccgagca cgaacaggcc cgtgctggcc gcaccgacca gcagcgcgag
     1621 cgcccagtag agcgcccagc ggccggccac gccgagcagc ggctgcgacg aggaccagcc
     1681 cgacgagccc gacgcggacg acggcgccgg cgcggccgtg gccgcgggtg cccgccgggc
     1741 cgccggcgcg ccggacgccg tccccccgcc cgcgctcagg gtgtaggtga acgcgccgct
     1801 ggcgacgtgg ccgtcgtcct tggagaccga gcgccagcgc accgtgtagg cgccgggcgc
     1861 cttggccagc tgggcggcga cgatcacggc gcggagttgc agcggggcgc ccatcaccgc
     1921 catggtctgc ccggagagct gccggccgtg cgcgtcgaag acatggatcg tggagagggt
     1981 cgggtcggcc tcctcggtga aggtgatcac gacctgctgg ggcgcccgcg tgagcgccgc
     2041 cccgcccgcc ggggtggagc cgcggagctc cgcgtgggcg tccgccaccg ccaccgcgcc
     2101 ggtcacgagc gggagcagcc acgccgcggc ggtcacgagc agcgcgagga tggtcctcct
     2161 gagcatcggc ctcacctctg ggtctcatct ccgggggctg aaatgagaga ggtcattatc
     2221 atctgtaatg cctatcgatg tcgagtcgaa cgaacaaatt cctgatagtt ggcgccccct
     2281 cgacgcatcc gagcgttttg ctcccgagac cgcccgcgct ccatggtcat gtccgaaacc
     2341 cgccagcaga ctgtcggggg caggcgccac aatcgaccca tggaacacga tttcgatacc
     2401 ctttaccggg cggcacagag ccgcgacccg cgctttgacg gacgcttcta caccgcggtc
     2461 acctcgacgg ggatctattg ccggccggtc tgcccggcgc agacgccgcg gcccgagaac
     2521 gtgcgcttct accagtgcgc ggcggcggcc gaggcggccg ggttccgcgc ctgccgccgc
     2581 tgccggccgg acaccgcacc cggctccgcc gaatggaacg tgcgcggcga cctggccgcg
     2641 cgggcgctgc ggctgatcgc cgccggggtg gtcgacggcg agggggtcga cgggctcgcc
     2701 agccgcctcg ccaccagccc gcgccacctg caccgccagc tcgtcgccga ggtcggcgcg
     2761 gggccgctcg ccttggcccg cacccggcgg gcgcagaccg cgcggctgct gatcgaccag
     2821 accgacctgc ccgtcaccga cgtggccttc agcgccgggt tcgcgagcat ccgccagttc
     2881 aacgacacca tccgcgccgc cttcgggacc accccgacgg agctgcgccg ccgccgtcgg
     2941 ccgctgctcg actcgaccgg cgggatggtg ctgcggctgc gccaccggac cccgttcgac
     3001 gccgacgggc tgctcggcta tctcgggcag cgggccctgc cgggcgtgga ggaggcagcg
     3061 ggcgggacct tccggcgggt gctggtgctg ccccgctcga ccgccgtggc cgagctggac
     3121 ctggttgaca acggcgccga ggtgcggctg cggctccgcc tcgacgacct gcgcgacctg
     3181 agcgtggcgg tgcagcgctg ccgctggctg ctagacctgg acgccgaccc ggccgcgatc
     3241 ggcgaggtgc tcggcggcga cccgctgctg gcgccgctgg tcgccgcgcg gccagggctg
     3301 cgcgtgcccg gcgccgtggc cgggctcgag ctggcggtcc gcgccatcct cggccagcag
     3361 atctcggtgg cgggggcgcg gaccctggcc gggcggctgg tcaacgccct cggcgagccg
     3421 ctgccgcagg ccgacggctc cctcacccac cgcttcccca ccgccgaggc gctggccacc
     3481 ctcgacccgg ccacgcccca gccaggcctc gggctgacca gggggaggct ggtggccctg
     3541 cgaggcctcg gccaggcggt cgccgccggc gagctggacc tcgaccgggg cgcggaccgg
     3601 gagcagaccg aaaagaccct gctcgcgctc cccggcatcg gcccgtggac cgccgcctac
     3661 atcgccatgc gcgccctcgg cgaccccgac gcgctgcccg cggccgacct cggcctgcgc
     3721 cgcgcgctgg aacgccacgg cctccccggc gacccggcca gcatccgcgc ccgcgccgaa
     3781 cgctggcgcc cctggcgcgc ctacgccgtc ctgcacctgt gggccggaac cgccggaacc
     3841 gccggaccgc cgcggcctcc acaagaacca gcacaagaac ccgcacccga gacggagaag
     3901 gagcctgtcg catgaacctc accaccgcca ccaccgaaac cccgatcggc ccgttcacgg
     3961 tgatcaccgg cgacggcgac gacggcgagg gtcccgtcgt gctcgccgcc ggcttcaccg
     4021 accagcccga ggagctgcgc gtccgcctcc acaaggactg gcgcaagctg ccgctggcgc
     4081 gcgccgaccg cctcggcgag gtctcggacg cgctcgacgc ctacttcgcc ggcgacctga
     4141 aggccctgga cggcctgccg gtgaagcagg tcaacaacga ccggatgctg cgcgcctggg
     4201 cgggtctccg cacgatcccg gccggcgaga cgatcagcta ccgcgacttc ggcgtcactg
     4261 tcttcggcga cccgagctac gcccgcgtgg ccggcggagc ctgcgccagg aacctggtcg
     4321 ccctgatcgt cccctgccac cgggtgatcc gcaccgacgg caacctcggc ggctactact
     4381 acggcctgga ccgcaagcgc tggctcctcc agcacgaacg tacccaggac caggcataac
     4441 tcgctccagc gacccgcgcc gccagggacg tccctggcgg cgcgtttcgt tgccacgtct
     4501 ttacactacc ttcggggggg aatgggcgaa gccgcgactc tcggaggatc aggcgccatg
     4561 gctgacacct acgtgcaggt cacgaccacc accgattcgc gcgacgacgc caagtggctg
     4621 gcacgggacg tggtggagcg ccgcctcgcc gcctgcgccc aggtcctcgg cccgatcgac
     4681 agcgtctact ggtgggaggg cgcggtcgag caggccgccg agtggctctg cctgatgaag
     4741 acgaccaccg gccgcctcga cgccctgatc gcccacctgc gatccgggca cagctacgac
     4801 aaccccgaga tcaccgccac cccgatcagc gccggcagcg ccgactacct cacctggatc
     4861 agccaggaga cggggaccgc cgcaggctcc tgacggcagg gtccgataag ctgcgggcga
     4921 acttggcgaa cttggatccg cccaatcctg tccggctcac cccgagggga cccgtatcat
     4981 gaccgaccac tcggccctgc acctgcaaca gtccgacccg gagatcgccc agctcgtcgc
     5041 cgccgaggag cggcggcagg cggagaagat ccggctgatc ccgtcggaga actacgtctc
     5101 ccgcgcggtg ctggaggcga ccggcacggt gctcaccaac aagtactccg aaggctaccc
     5161 gggcaagcgc tactacgagg gccagcagat cgtcgaccag gtcgagacgc tggcgatcga
     5221 gcgcgccaag gcgctgttcg gcgtcgacca cgccaacgtg cagccctact cgggctcccc
     5281 cgccaacttc gccgtctact tcgccttcct ggagccgggc gacacggtga tggggatggc
     5341 gctgccgatg ggcggccacc tgacccacgg ctggaacgtg tcggtcaccg gcaagtggtt
     5401 ccgctcggtg cagtacggcg tgcgcaagga caccggccgg gtggacctgg acgaggtccg
     5461 cgagctcgcc ctcaaggagc ggccgaagct gctgttctgc ggcggcacgg cgatcccgcg
     5521 cacgatcgac ttcgccggct tcgccgccat cgccaaggag gccggcgcgc tgctggtcgc
     5581 cgacatcgcc cacatcgccg ggctgatcgc cggcggcgcc caccccaccc cggccgggca
     5641 cgccccggtg atctccacca ccacccacaa gacgctgcgc ggcccgcgcg gcgcgatgct
     5701 gatgagcgac gccgagcacg ccaaggcgct cgaccgctcg gtcttccccg gcctgcaggg
     5761 cggcccgcac aaccacacca ccgccgccat cgcggtcgcc ctgc
//
LOCUS       DASWDC010000299         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_736526, whole genome shotgun sequence.
ACCESSION   DASWDC010000299 DASWDC010000000
VERSION     DASWDC010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736526"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VF995_09660"
     CDS             <1..777
                     /locus_tag="VF995_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9343865.1"
                     /translation="WGGPVFGQIATHVIPPTMLSGQLGQSYDPYATSGFHGDLAKAQA
                     EMRQSRYDANKDGVCDSPVCRNLVMVSRNVAPFIGMEPIITQDLAKIGIQVTAREYST
                     SAAYRTIQSVKDDIPLALHPQWIKDYADPFTFANPLFGSGSIIPEGNVNYSMVGLTSG
                     MAAKLGIAFPSGATIPNVDSDINACEQIPASEPTRTSCWVNFDKKLMEQVVPWVPYLW
                     GDNITITAPSVTNYVYDQSSSYISLTQLAVNNKVSASSLG"
     gene            complement(827..2347)
                     /locus_tag="VF995_09665"
     CDS             complement(827..2347)
                     /locus_tag="VF995_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX9343866.1"
                     /translation="MNPRLKAICDLDVAVTREYVGLHDDYDGYVPDLSPDGVRARVAR
                     LGLPGNDGDLLTEPDPHDETRVRTVEAALRYELGELQSHRRNPLWHIAELDLSSYDRD
                     YAPAGQRAEARHRHLATWPDAIDAAIASLDRIPAPVAVALLPAARGLAAGLDGDRATL
                     DAALAAHQRFVVHLEAAAEHGDPDPALGRGVLERLLSAREATAVDLGRLEERADAERD
                     RLRALLTDACGRLQAGTKPAEIVAELLRDHPDAGGVLDEAQAATDEVIAFTIERGLVP
                     GLDGACLVAPAPPSRRWALAMMSWAAPYEADAPSRYYISPPDPSWPAEEQGQWLAVFS
                     RTSLPAITAHEVAPGHFAHGRCIRKVSSDVRRTLHSDAFVEGWAHYAEELCLEEGLRA
                     TDPRFAAGVALEALQRVTRLAVSIGLHSGTMPLDEAIARFEADAFVYGPAARAEATRA
                     TFDPTYGRYTWGKLEILRLREQAKATWGSGYSVQRFNAALLALGSPPLGLMEHALS"
     gene            complement(2387..>2520)
                     /locus_tag="VF995_09670"
     CDS             complement(2387..>2520)
                     /locus_tag="VF995_09670"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX9343867.1"
                     /translation="THEVKRLYVADSSVFPTASGVNPMLSIMGLAHRAAGRIAARLG"
BASE COUNT          368 a          831 c          959 g          362 t
ORIGIN      
        1 tggggcgggc cggtattcgg ccagatcgcc acccacgtga tcccgccgac gatgctgtcc
       61 ggccagctcg gccagtccta tgacccctat gcgaccagcg gtttccacgg cgacctcgcc
      121 aaggcccagg cggagatgcg ccagtcgcgc tatgacgcca acaaggacgg tgtctgcgat
      181 tcgccggtgt gccgcaacct ggtgatggtg agccgcaatg tggcgccgtt catcggcatg
      241 gagccgatca tcacccagga cctcgccaag atcggcatcc aggtgactgc ccgcgagtac
      301 tccaccagtg ccgcctaccg cacgatccag tcggtcaagg atgacatccc gctggcgctg
      361 cacccccagt ggatcaagga ctacgccgac cccttcacct tcgcgaatcc gctgttcggc
      421 tctgggtcga tcatccccga gggcaacgtg aactactcga tggtcggcct cacctcgggc
      481 atggccgcca agctcgggat cgccttcccc agcggcgcca ccatccccaa tgtcgacagc
      541 gacatcaacg cgtgtgagca gatccccgcc agcgagccga cccggaccag ctgctgggtc
      601 aactttgaca agaaattgat ggagcaggtg gtgccctggg tgccgtacct gtggggcgac
      661 aacatcacga tcacggcacc gagcgtcacc aactacgtgt acgaccagtc gtcgagctat
      721 atctcgctga cgcagctcgc tgtcaacaac aaggtgagtg cgtcctcgct cggctgaccg
      781 tcgcggcggt gcgccgcccg tgcccgcgcc cgcgccgctg gcacggtcag ctgagggcgt
      841 gctccatcag gccgagcggg ggtgagccga gcgccagcag ggcggcgttg aagcgctgca
      901 cggagtagcc gctgccccag gtggccttgg cctgttcgcg aaggcggagg atctcgagtt
      961 tgccccaggt gtagcggccg taggtggggt cgaaggtggc gcgggtcgcc tcggcacggg
     1021 cggccgggcc gtagacgaac gcgtcggctt cgaagcgggc gatggcttcg tccagcggca
     1081 tggtgccgct gtggaggccg atcgagacgg ccaggcgggt gacgcgctgc agcgcctcca
     1141 aggcgacgcc ggcggcgaag cgggggtcgg tggcgcgcag gccctcctcc aggcacagct
     1201 cctcggcgta gtgggcccag ccttcgacga aggcgtcgga gtggagggtg cggcgcacgt
     1261 cgctgctgac cttgcggatg cagcggccgt gggcgaaatg tccaggagcg acttcgtggg
     1321 cggtgatcgc gggcaagctc gtgcggctga acaccgccag ccactgcccc tgctcctcgg
     1381 ccggccagga cgggtccggc ggggagatgt aatagcggga gggcgcgtcg gcctcatagg
     1441 gcgcggccca cgacatcatc gccagcgccc agcgccgcga cggcggcgct ggcgcgacca
     1501 ggcacgcgcc gtcgagaccc ggaaccagcc ctcgctcaat cgtgaaggcg atgacctcgt
     1561 cggtggcggc ctgggcctcg tcgagcacgc cgccggcgtc ggggtggtcg cggagcagct
     1621 cggcgacgat ctcggcgggc ttggtgccgg cctggaggcg gccgcaggcg tcggtgagga
     1681 gggcgcggag ccggtcgcgt tcggcgtcgg cacgctcttc caggcggccg aggtcgaccg
     1741 ccgtcgcctc cctggccgac aggagccgct ccaggacgcc ccggccgagc gcggggtcgg
     1801 ggtcgccgtg ctcggcggcg gcttccaggt gcacgacgaa gcgctggtgg gcggcgaggg
     1861 cggcgtccag ggtcgcccgg tcgccgtcga ggccggcggc gagcccgcgg gcggcgggca
     1921 gcagcgccac ggcgactggg gcggggatcc ggtcgagcga ggcgatggcg gcgtcgatcg
     1981 cgtcgggcca ggttgccagg tggcggtggc gggcctcggc gcgctgccca gcgggggcgt
     2041 agtcgcggtc gtagctggac aggtccagct cggcgatgtg ccacagcggg ttgcggcggt
     2101 gggactgcag ctcccccagc tcataccgga gggccgcctc gacggtgcgc actcgcgtct
     2161 cgtcgtgggg gtccggctcg gtgagcaggt cgccgtcgtt gccggggagg ccgagacggg
     2221 caacgcgggc gcgcacgccg tcgggtgaaa ggtcggggac gtagccgtcg tagtcgtcgt
     2281 ggagaccgac gtattccctg gtgacggcca cgtcgaggtc gcagatggcc ttgagtcgcg
     2341 ggttcatcgc gtcggagggt agcagacgag gccgctcgcc aatcgcctag ccaaggcggg
     2401 cggcgatccg gccggcggcg cggtgggcga ggcccatgat ggacagcatc gggttcaccc
     2461 cggaggcggt cgggaagacc gacgagtcgg ccacgtagag gcgcttgacc tcgtgggtct
//
LOCUS       DASWDC010000300         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1415745, whole genome shotgun sequence.
ACCESSION   DASWDC010000300 DASWDC010000000
VERSION     DASWDC010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415745"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2170)
                     /locus_tag="VF995_09675"
     CDS             complement(<1..2170)
                     /locus_tag="VF995_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006347143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9343868.1"
                     /translation="MDTTTNADTPAPAAAAAAAHAPAPEASPDLLDGLNDAQRRAVLS
                     TDGPTLIVAGAGSGKTRVLTHRIAHLVKDRGVHPSSILAITFTNKAAKEMRERLGGLV
                     GPVVKAMWVSTFHSACVRILRRSGSALGYTSNFTIYDESDAERLMAVVCRELNFDPKR
                     LAPRAMRHAVSSAKDDLLDPGAFADRAGTWFEKKVAEAYDRYQRRLTEANAVDFDDLI
                     MQVVRLFREHPEVLGEYQRRFRYLLVDEFQDTNVAQYVLLKLLSAEHRNLSVVGDSDQ
                     SIYAFRGATIRNILDFEADYPEANVILLEQNYRSTQTILDAANAVISNNLERKPKSLW
                     TALGGGHPIVRYAAETEHDEAAYVAEEAERLHDRGVGYGQVVIFYRTNAQSRVLEEVF
                     VKAGMPYKVVGGVKFYDRREVKDLLAYLKAVVNPLDTVSLRRVLNVPKRGIGETSERA
                     IDRFAEQEGIPFGEALRRAGEVPGLGNRQTRSTLDFVAVLDELRAHMADGWAPSVLVE
                     DAWKLSGLLRELEADTSIEGQGRVENVKELQSVAEEYELREPEGGLGGFLESIALVSD
                     ADQVESGSAAVTLMTLHTAKGLEYPVVFMVGMEENVFPHIRSIGEPRELEEERRLAYV
                     GITRARERLYLTSAWSRSLFGSTNYNLPSRFLKEIPDELVTVAGPSAPSSRSGSWGGS
                     GTGSGGGRGAERGVAFGAGRSTFGPRGQDAARRAGPPPVSF"
     gene            2299..2619
                     /locus_tag="VF995_09680"
     CDS             2299..2619
                     /locus_tag="VF995_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEX9343869.1"
                     /translation="MIIATTNDLPGYQVTEVLGEVFGLTVRSRNIGSQLGAAFKSIAG
                     GELKGMTKNLINSRHEAIARMVAEAEAKGANAVLAMRFDTSEMGDVWTEIAAYGTAVK
                     VQPA"
BASE COUNT          398 a          933 c          894 g          397 t
ORIGIN      
        1 cgaagctgac cggcggcggg ccggcgcgcc gcgccgcgtc ctggccccgg gggccgaagg
       61 tggagcgccc cgccccgaac gccacgccgc gttcggcgcc gcgcccgccg cccgaacccg
      121 tcccagagcc gccccacgac cccgagcggc tcgacggcgc cgacgggccg gcgacggtga
      181 ccagctcgtc ggggatctcc ttgaggaagc gggacggcag gttgtagttg gtcgagccga
      241 acagggagcg gctccaggcg ctggtcaggt acagccgctc gcgcgcccgg gtgatgccca
      301 cgtaggccag ccggcgttcc tcctcaagct cgcgcggctc gccgatcgag cggatgtggg
      361 gaaagacgtt ttcctccatg ccgaccatga acaccaccgg gtactcgagc cccttggcgg
      421 tgtgcagcgt catcagcgtc accgcggcgc tgccgctctc gacctggtcg gcgtcggaga
      481 ccagcgcgat cgactcgagg aacccgccga ggccgccctc tggctcgcgc agctcgtact
      541 cctcggcgac cgactggagt tccttgacgt tctcgacccg gccctggccc tcgatggagg
      601 tgtcggcctc cagttcgcgc agcaggcctg acagcttcca ggcgtcctcg accagcacgc
      661 tcggcgccca gccgtcggcc atgtgggcac gcagctcgtc gaggacggcg acgaagtcga
      721 gggtggacct cgtctggcgg ttgccgaggc cgggcacctc gccggcgcgg cgcagcgcct
      781 cgccgaaggg gatgccctcc tgctcggcga agcggtcgat ggcccgctcg gaggtctcgc
      841 cgatgccgcg cttgggcacg ttcagcaccc gccgcaggct caccgtgtcg agcgggttga
      901 ccaccgcctt caggtaggcc agcaggtctt tgacctcgcg ccggtcgtag aacttgacgc
      961 cgccgaccac cttgtagggc atgccggcct tgacgaagac ctcttcgagc acccgggact
     1021 gcgcgttggt gcggtagaag atcaccacct ggccgtagcc cacaccccgg tcgtggaggc
     1081 gctcggcctc ttcggcgacg taggcggcct cgtcgtgctc ggtctcggcg gcgtagcgga
     1141 cgatcgggtg gccgccgccg agggcggtcc agaggctctt gggcttgcgc tccaggttgt
     1201 tggagatgac ggcgttggcg gcgtccagga tcgtctgggt ggagcggtag ttctgctcca
     1261 gcaggatcac gttcgcctcc gggtagtccg cctcgaagtc gaggatgttg cggattgtcg
     1321 cgccccggaa cgcgtagatg ctctggtcgc tgtcacccac caccgagagg ttgcggtgct
     1381 cggcagacag cagcttcagc agcacgtact gggcgacgtt ggtgtcctgg aactcgtcga
     1441 ccagcaggta gcggaagcgc cgctggtact cccccagcac ctcgggatgc tcgcggaaca
     1501 ggcgcaccac ctgcatgatc aggtcgtcga agtcgaccgc gttcgcctcc gtcaggcgcc
     1561 gctggtagcg gtcgtaggcc tcggcgacct tcttctcgaa ccaggtgccg gcgcggtcgg
     1621 cgaacgcgcc ggggtcgagc aggtcgtcct tggccgagga gaccgcgtgg cgcatcgccc
     1681 gcggcgccag ccgtttcggg tcgaagttca gctcgcggca gaccaccgcc atcagccgct
     1741 cggcgtcgga ctcgtcgtag atggtgaagt tcgacgtgta gccgagcgcc gacccgctgc
     1801 gccgcaggat ccgcacgcag gccgaatgga acgtcgagac ccacatcgcc ttcaccaccg
     1861 gcccgaccag gccgccgaga cgctcccgca tctccttggc cgccttgttg gtgaaggtga
     1921 tcgccaggat cgacgaggga tgcacccccc ggtccttgac cagatgcgcg atccggtggg
     1981 tgagcacccg cgtcttgccc gaccccgcac ccgccacgat cagggtcggc ccgtcggtcg
     2041 acagcaccgc ccgccgctgc gcgtcgttca gcccatcgag caggtcaggg gatgcctctg
     2101 gtgctggggc gtgcgctgcg gccgcagctg cggctggagc tggggtgtcg gcgttcgttg
     2161 tcgtgtccac gtggcgaagt atacgggcca ccgctgacca cccggagcgg ttgtccacac
     2221 ccacaaccgg cgactcggat aggctcagac gcagaaccag acagacacgg aagcaagccc
     2281 tgccacaagg aggcacgcgt gatcatcgcc accaccaacg acctgcccgg ctaccaggtc
     2341 accgaggtcc tcggggaggt cttcgggctg acggtccgct cccgcaacat cggctcccag
     2401 ctcggcgccg ccttcaagtc catcgccggc ggcgaactga agggcatgac caagaacctg
     2461 atcaacagcc gccacgaggc gatcgcccgc atggtcgccg aagccgaggc caagggcgcc
     2521 aacgccgtcc tcgcgatgcg cttcgacacc tccgagatgg gcgacgtctg gaccgagatc
     2581 gccgcctacg gcaccgccgt caaagtccag cccgcctgag cc
//
LOCUS       DASWDC010000301         4275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_583774, whole genome shotgun sequence.
ACCESSION   DASWDC010000301 DASWDC010000000
VERSION     DASWDC010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583774"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1591
                     /locus_tag="VF995_09685"
     CDS             275..1591
                     /locus_tag="VF995_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX9343870.1"
                     /translation="MRTADRGRRRRGRGLTEEQKQVLRQGPEKRMLVTEGADRTQIGV
                     IEGSSLVEHYVTRKSGRSMVGNIYLGRIQNVLPGMEAAFIDIGRGRNAVLYAGEVNYN
                     EEDLEGEAPRIERVLKPGQPVLVQVTKDPIGAKGARLTAQVSLAGRYLVLQPGDTTFG
                     ISRRLPEEERIRLREILKEVRPKGLGLIVRTAAEGASADDLRADLARLQARWESIERK
                     SKKSSAPAVIYSEPELVVRVIRDIFSPDFAELVVDSPELHARVKDYLDEVAPDLLPKL
                     RHHDGKLPLFEHYRVAEQIHKALERKVWLPSGGSLVIDQTEAMTVVDVNTGKFVGKTN
                     LEETVVATNLEAAEETVRQLRLRDIGGIIIIDFIDMLFEKNQHAVVERLRDALARDKT
                     KSQVMEVSSLGLVQMTRKRVSGGLLENFSEPCPACEGRGLVITHEV"
     gene            1616..2026
                     /gene="rplU"
                     /locus_tag="VF995_09690"
     CDS             1616..2026
                     /gene="rplU"
                     /locus_tag="VF995_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006064165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX9343871.1"
                     /translation="MAPEFPDSSRKDDRIVYAVIRTGGKQYKVAVGDLIDVERLAGDD
                     GASISFAPILVVDDEGKVTSAAADLAKAAVTASVVDQHRGKKIRIFTYKNKTGQQRHM
                     GHRQSLTRLKVEGIQLGAGRRSSTRASKKTESES"
     gene            2028..2282
                     /gene="rpmA"
                     /locus_tag="VF995_09695"
     CDS             2028..2282
                     /gene="rpmA"
                     /locus_tag="VF995_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017583463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX9343872.1"
                     /translation="MAHKKGASSSRNGRDSAAKRLGVKRYGGQLVTAGTILVRQRGTR
                     IHPGTGVGRGGDDTLFAKIDGHVVYHQIRGRKHASIDPRA"
     gene            2427..3449
                     /locus_tag="VF995_09700"
     CDS             2427..3449
                     /locus_tag="VF995_09700"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX9343873.1"
                     /translation="MNLEQALFPMTLERGAARRRLAELQGHVICAGPEAWERVKDVAN
                     PPLQVITAGSIELADLTRLAAYVPEDAKVVVGLGGGTSLDTAKWVAAARALDLVQIPT
                     VISVDAAFSPSWGYRDRERLQYAGHVVPGEVIVDPELIGQSSPPLNRAGVGDLLSCHT
                     ALVDWRLAADAGQGVPWDEELATIAGIVLDELEAAAPEIKAMSDQGIGFIAGAHRLVG
                     AARQAARHARFEEGSEHFLAYVVEWLSGRRPAHGELIAFCVLVMSYVQGEDTDRAVRI
                     VRETGVDARPGHLGVDGPLLARCFAELPDYAVREHLDASVIELVELAPGLASSAFAHA
                     CEATGD"
     gene            3485..>4275
                     /gene="obgE"
                     /locus_tag="VF995_09705"
     CDS             3485..>4275
                     /gene="obgE"
                     /locus_tag="VF995_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006896651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX9343874.1"
                     /translation="MFVDETTIEVTAGRGGDGVTSFHREKYRPRGGPDGGNGGRGGDV
                     VLVAEPGIATLVEYHFHPHQRAQKGRHGQGSNRQGGAGDDLLLPVPVGTLVRDEQGTL
                     LVDLAWPGARFVAAKGGRGGRGNTVLTVGRRVAHFHEKGEPGRRRRLTLELRLLADAG
                     LVGFPNAGKSSIVGFASAAKPRVADYPFTTLEPVLGVVRAGAYDYVLADVPGLIPGAA
                     AGKGLGHRFLRHVTRSAVLVHVLDLAPLEPGRDPEADLAALEAELA"
BASE COUNT          659 a         1567 c         1484 g          565 t
ORIGIN      
        1 cgagatcgtc ggcgatgatc aatagaatgt ttggtttgtc ggggcctgag ctggtgccgc
       61 cgctgccgcc gctgccggca ccagcgccgc gccggccgcc gagcaaaggc agccgacccg
      121 cacccgcagt cgtggccgcc gcctcacccc cggccacagc aaggggcggg agagccaggc
      181 caacggcgcg accgccgccg ccacgccctc cccccaggca cccgcaccgt ccgagcgctc
      241 cacccggctg gaggccggca cccgcacccg cggggtgcgg acggccgacc ggggccggcg
      301 ccgccgcggc cgcggcctga ccgaggagca gaagcaggtc ctgcggcagg gcccggagaa
      361 gcgcatgctg gtcaccgagg gggccgaccg cacccagatc ggcgtgatcg aaggctccag
      421 cctggtcgag cactacgtga cccgcaagtc cggccgctcc atggtcggca acatctacct
      481 cggccgcatc cagaacgtcc tgcccggcat ggaggctgcc ttcatcgaca tcgggcgcgg
      541 ccgcaacgcc gtgctctacg ccggcgaggt caactacaac gaagaggacc tcgagggcga
      601 ggcgccccgg atcgagcggg tcctcaagcc tggccagccc gtgctggtcc aggtcaccaa
      661 ggacccgatc ggcgccaagg gcgcgcgcct caccgcccag gtctcgctcg ccggccgcta
      721 cctggtcctg cagcccggcg acaccacctt cggcatctcc cggcggctgc cggaggagga
      781 gcgcatccgc ctgcgcgaga tcctcaagga ggtgcgcccc aagggtctcg gcctgatcgt
      841 gcgcaccgcc gccgagggtg ccagcgccga cgacctgcgc gccgacctcg cccggctgca
      901 ggcccgctgg gagtccatcg agcgcaagtc caagaagtcc tcggccccgg cggtgatcta
      961 ctccgagccg gagctggtgg ttcgggtgat ccgcgacatt ttctcgcccg acttcgccga
     1021 gctggtggtc gactcgcccg agctgcacgc ccgcgtcaag gactacctcg acgaggtcgc
     1081 gcccgacctg ctgccgaagc tgcgccacca cgacggcaag ctgccgctgt tcgagcacta
     1141 ccgggtggcc gagcagatcc acaaggcgct ggagcgcaag gtctggctcc cgtccggcgg
     1201 ctcgctggtc atcgaccaga ccgaggcgat gaccgtggtc gacgtcaaca ccggcaagtt
     1261 cgtcggcaag accaacctgg aagagaccgt ggtcgccacc aacctcgagg cggccgagga
     1321 gaccgtgcgc caactccgcc tgcgcgacat cggcggcatc atcatcatcg acttcatcga
     1381 catgctcttc gagaagaacc agcacgccgt ggtcgagcgc ctgcgcgacg ccctcgcccg
     1441 cgacaagacc aagagccagg tgatggaggt ctcctccctc ggcctggtgc agatgacccg
     1501 caagcgggtc tccggcggcc tgctggagaa cttctccgag ccctgcccgg cctgcgaggg
     1561 ccgcggcctg gtcatcaccc acgaggtctg atgtcgccgg cgccattgag ctaagatggc
     1621 gcccgaattt cctgacagca gtcgaaagga cgatcgcatc gtgtacgccg tcatccgaac
     1681 cggcggaaag cagtacaaag tcgcggtcgg cgacctcatc gacgtcgagc ggcttgccgg
     1741 cgacgacggc gccagcatct ccttcgcgcc catcctggtc gtcgacgacg agggcaaggt
     1801 gacctccgcc gccgccgacc tcgccaaggc agccgtgacc gccagcgtgg tcgaccagca
     1861 ccgcggcaag aagatccgca tcttcaccta caagaacaag accggccagc agcgccacat
     1921 gggccaccgc cagtccctca cccgcctcaa ggtcgagggt atccagctcg gcgccggccg
     1981 ccggtcctcc accagagcat cgaagaagac ggagagcgag agctgacatg gcccacaaga
     2041 agggggcgtc ctccagccgc aacggccgcg actccgccgc caagcgcctc ggcgtgaagc
     2101 gctacggcgg ccagctggtc accgccggga ccatcctggt ccgccagcgc ggcacccgca
     2161 tccaccccgg caccggcgtc ggccgcggcg gcgacgacac cctgttcgcc aagatcgacg
     2221 gccacgtcgt ctaccaccag atccgcggcc gcaagcacgc cagcatcgac ccccgcgcct
     2281 gaggcacccg cctcggcggc tcactggccc tcggttcgag ggccagtcgc gtctctctca
     2341 caccacgtgg aaccaccagg tatgcgagga tttgggcgtc gacgtccccc agatggctga
     2401 caggtcgcgt gagatgatgc cctgagatga acctggagca ggcactgttt cccatgacgc
     2461 tcgaacgggg agcggcacgg cggcgcctcg cggagttgca gggccacgtc atctgcgccg
     2521 ggccggaggc gtgggagcgg gtcaaggacg tggcgaaccc gcccctgcag gtcatcaccg
     2581 cgggcagtat cgagctggcg gacctcaccc ggctggcggc ctatgtcccg gaggacgcca
     2641 aggtggtggt cgggctcgga ggcgggacca gcctcgacac cgccaagtgg gtggcggcgg
     2701 cccgggcgct cgacctggtc cagatcccca cggtgatctc ggttgacgcc gccttctcgc
     2761 cgagctgggg ctaccgcgac cgcgagcggt tgcagtacgc ggggcacgtc gtgcccggcg
     2821 aggtgatcgt cgacccggag ctgatcgggc agtcgtcacc gccactgaac cgggccggcg
     2881 tcggcgacct gctctcctgc cacaccgcgc tggtcgactg gcggctggcc gccgacgccg
     2941 gtcagggcgt gccctgggac gaggagctgg ccaccatcgc cgggatcgtg ctggatgagc
     3001 tggaggccgc cgcgcccgag atcaaggcca tgtccgacca ggggatcggc ttcatcgccg
     3061 gtgcgcaccg gctggtcggg gccgcgcgtc aggccgcccg gcatgcccgc ttcgaggagg
     3121 gcagtgaaca cttcctcgcc tacgtcgtcg agtggctcag cggccgccgg cccgcccacg
     3181 gcgagctgat cgccttctgt gtgctggtga tgagttacgt ccagggcgag gacaccgacc
     3241 gcgccgtacg gatcgtccgc gagaccggcg tcgacgcccg tccagggcat ctcggcgtcg
     3301 acggcccgct gctcgcccgc tgcttcgccg agctgcccga ctatgccgtc cgcgagcacc
     3361 tggacgccag cgtcatcgag ctggtcgagc tggcccccgg cctggcctcc tccgccttcg
     3421 cccatgcctg cgaggcgacc ggcgactgac cctgggctgg cgggaagcct gctaggctcg
     3481 gcgcatgttt gtggacgaaa cgacgatcga ggtgaccgcc gggcggggcg gcgacggggt
     3541 gacctcgttc caccgggaga agtaccggcc gcggggcggg ccggacggcg gcaacggcgg
     3601 ccgcggcggc gacgtcgtgc tggtcgccga gccgggcatc gccacgctgg tcgaatacca
     3661 cttccacccg catcagcggg cccagaaagg ccgccacggc cagggcagca accgccaggg
     3721 gggggcgggc gacgacctgc tccttccggt gccggtcggt accctggtgc gcgacgagca
     3781 gggcaccctc ctcgtggatc ttgcctggcc aggggcccgg ttcgtggccg caaagggggg
     3841 ccgtgggggg cggggcaaca ccgtgctgac ggtcgggcgg cgggtggcgc acttccacga
     3901 gaagggggag ccgggcaggc ggcggcgcct caccctggag ctgcggctgc tcgccgacgc
     3961 cgggctggtc gggttcccca acgctggcaa gtcgagcatc gtgggcttcg cgtcggcggc
     4021 caagcccagg gtggccgact accccttcac caccctggag ccggtcctcg gcgtggtccg
     4081 tgccggggcg tatgactacg tgctcgccga cgtgcccggc ctgatccccg gcgccgccgc
     4141 cggcaagggc ctcggccatc gcttcctgcg tcatgtgacc cgttcggcgg tattggtcca
     4201 cgtcctggac ctggccccgc tggagcccgg ccgcgacccc gaggcagacc tggccgcgct
     4261 ggaggcggag ctggc
//
LOCUS       DASWDC010000302         2571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_333635, whole genome shotgun sequence.
ACCESSION   DASWDC010000302 DASWDC010000000
VERSION     DASWDC010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333635"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VF995_09710"
     CDS             <1..562
                     /locus_tag="VF995_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9343875.1"
                     /translation="AMGLEVAMLTGDNATTAAVIAASVGIDRVLAEVMPADKVGEIAR
                     LQAEGKTVAMVGDGVNDSPALVQADLGIAIGSGTDVAIESSDLTLLRGDLDGVATAIE
                     LSRRTYRTIVQNLGWAFGYNIVAIPLAIAGLLNPIIAGAAMAFSSVSVVSNSLRLRRL
                     DVSGRTDGGGRGHRLGAVYGSCRLRQ"
     gene            658..>2571
                     /locus_tag="VF995_09715"
     CDS             658..>2571
                     /locus_tag="VF995_09715"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9343876.1"
                     /translation="MEPKPKEQSTVVLDVRGLNWASEKAVVEAVLSRRPGVLAVEANP
                     VSQTATVTYDPRLTSLVELRRWVTECGYHCAGQSVPAHVCDPMLEPDPPSGGHADHGA
                     AAPTSPVSPQSAEPPAPPAHAGPAPAHAGHAPSPEEAVRAPEEVMGHGGHAGMSMAAM
                     VADMRNRFLVAAIFSVPILLWSPIGRDVLGFTVAAPFGLRDDVWMLLLSLPVIFYSCA
                     IFFDGAVRALRARTLDMMVLVAVAVGAGWLYSLYVTLTGGGDVFYEAATVLAAFVLLG
                     HWFELRARGGANDAIRTLLDLAPPRALVLRDGEPAEVPTAEVEVGDLLLVRPGAKIAV
                     DGVVTDGDSEVDESMVTGESLPVHKQPGDQVIGATINKNGTLRVRATKVGADTALAQI
                     VRLVQEAQNSKAPGQRLADRAAFWLVLVALLGGGGTLATWLLVGRPLSEAILFAITVV
                     VITCPDALGLATPTAIMVGTGLGAGRGILFKNAMALETSARIQAVVMDKTGTLTKGEP
                     EVTDLVTADGVGQAELLRLVAAVERESEHPLAEAIVRRADQDGVAQVSAAGFENVPGH
                     GAVATVEGRRVAVGNLRLMERDGVELGSLSTRRQELAAGGRTVVVAAVDGRAAGLVAI
                     ADAPRPTAKAAVDA"
BASE COUNT          349 a          877 c          975 g          370 t
ORIGIN      
        1 cgccatgggc ctggaggtcg ccatgctcac cggcgacaac gccaccaccg cggccgtgat
       61 cgcggccagc gtcggcatcg accgggtgct tgcggaggtc atgcccgccg acaaggtcgg
      121 cgagatcgcc cgcctgcaag ccgagggcaa gaccgttgcg atggtcggtg acggcgtcaa
      181 cgacagcccc gcgttggtcc aggccgacct tgggatcgcg atcggcagcg gcaccgacgt
      241 cgccatcgag tccagcgacc tcaccctgct ccgcggtgac ctggacggcg tggccaccgc
      301 catcgagctg tcacggcgga cctaccgcac gatcgtgcag aaccttggtt gggcgttcgg
      361 ctacaacatc gtggccatcc cgctggcaat cgccggcctg ctcaacccga tcatcgctgg
      421 cgccgccatg gccttctcca gcgtcagcgt ggtcagcaat tccctgcgcc tgcgccgctt
      481 ggacgtcagc ggccggactg atggcggcgg ccgcggtcac aggctgggcg cggtgtacgg
      541 tagttgccgg ctacgacagt gagtcggacc atcgcggaag tcgcgcgggc gagaccttaa
      601 gccagcggca cgggttggcg gggagatgcg ccgattgccg gcgggggagg gagtgtcatg
      661 gagcccaagc ccaaggagca gagcacggtt gttcttgacg tccgcggtct caactgggcc
      721 agtgagaagg cggtggtgga ggcggtgctc agccggcggc caggtgtgct ggccgtcgag
      781 gcgaacccgg tctcccagac cgcgacggtg acctacgatc cccggttgac gtcgctggtc
      841 gagctgcgcc gctgggtgac cgagtgcggc tatcactgcg ctgggcagtc ggtgcctgcg
      901 cacgtgtgcg acccgatgct ggagcccgat ccaccgtcgg gcgggcacgc cgatcacgga
      961 gcggccgcac cgacatcgcc ggtttcgccg cagtcggcgg agccacccgc gccacccgcg
     1021 catgccgggc cagcgcccgc gcatgcgggc cacgctccgt cgccggagga ggcggtgcgc
     1081 gcgccggagg aggtgatggg ccacggcggg cacgctggca tgtcgatggc ggcgatggtc
     1141 gccgacatgc gcaaccgctt cctggtcgcc gcgatcttct cggtcccgat cctgctgtgg
     1201 tcgccgatcg gccgtgacgt gctcggcttc accgtggcgg cgccgttcgg gctccgtgac
     1261 gacgtctgga tgctgctgct gagcctgccg gtgatcttct actcgtgtgc gatcttcttc
     1321 gacggtgcgg tgcgcgcgct gcgcgcccgc acgctggaca tgatggtgct ggtcgccgtg
     1381 gcggtcgggg cggggtggct gtactcgctg tatgtcaccc tgaccggcgg cggggacgtc
     1441 ttctacgagg ccgccaccgt gctggccgcg ttcgtgctgc tcggccactg gttcgagctg
     1501 cgcgcccgcg gcggcgccaa cgacgccatc cgcacgctgc tggacctggc cccaccccgg
     1561 gcgctggtgc tgcgcgacgg ggagccggcc gaggtcccca ccgccgaggt cgaggtcggc
     1621 gacctgctgc tggtccgccc cggcgccaag atcgccgtgg acggggtcgt caccgacggc
     1681 gacagcgagg tcgacgagtc aatggtcacc ggcgagagcc tgccggtcca caagcaacca
     1741 ggcgaccagg tcatcggcgc gaccatcaac aagaacggca cgctgcgggt gcgggccacc
     1801 aaggtcggcg ccgacaccgc gctggcccag atcgtcaggc tggtgcagga ggcgcagaac
     1861 tccaaggcgc caggccagcg cctggcagac cgggcggcgt tctggctggt gctggtcgcg
     1921 ctgctcggcg gcggcgggac cctggccacc tggctgctgg tcggccgccc gctgagtgag
     1981 gcgatcctgt tcgcgatcac ggtggtggtg atcacctgcc cggacgcgct ggggctggcc
     2041 accccgacgg cgatcatggt cggcacgggt cttggcgccg ggcggggaat cctgttcaag
     2101 aacgccatgg cgctggagac ctcggcgcgg atccaggcgg tggtcatgga caagaccggc
     2161 accctgacca agggcgagcc ggaggtcacc gacctggtca ccgccgacgg ggtcggccag
     2221 gcggagctgc tgcgcctggt cgccgccgtc gagcgagagt cagagcatcc gctggccgag
     2281 gcgatcgtgc gccgcgccga ccaggacggc gtggcccagg tgtccgccgc cgggttcgag
     2341 aacgtgcccg gccacggggc ggtcgccacc gtggagggcc ggcgggtcgc ggtcggcaac
     2401 ctgcggctga tggagcgcga cggggtcgag ctgggcagcc tcagcactcg ccgccaagag
     2461 ctggccgcgg gcgggcgcac ggtggtcgtg gcggccgtcg acggtcgggc agcagggctg
     2521 gtggccatcg ccgacgcgcc ccggccgacc gccaaggcgg cggtggacgc g
//
LOCUS       DASWDC010000303        22796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_913487, whole genome shotgun sequence.
ACCESSION   DASWDC010000303 DASWDC010000000
VERSION     DASWDC010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22796
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913487"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VF995_09720"
     CDS             complement(<1..471)
                     /locus_tag="VF995_09720"
                     /inference="COORDINATES: protein motif:HMM:NF019220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9343877.1"
                     /translation="MSDLSVLWPGFLWALLLLPAALAAYLLAQRRRTRYAVRFTNLDL
                     LANVVTHSPRWRRHLPPALYLLALAALLLGMARPQAVTLVPKEQATVMLVMDVSGSMN
                     ATDVQPTRLLAAEQAATVFLNELPAKFRVGVVSFSGTAQTIARPTTDRQAVEDAI"
     gene            complement(468..1421)
                     /locus_tag="VF995_09725"
     CDS             complement(468..1421)
                     /locus_tag="VF995_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9343878.1"
                     /translation="MKATRLPERTPDRPGPGPTSEALLRALDVVVRRRVEALLTGEYR
                     SSQLGIGTELAQIRLYQPGDDVRRIDWNVTARTREPHVRDQVAERALVSWLVLDASPS
                     MSFGTAERRKYDVAEGVALAVGHIATRRGNRLGLVSFGNEESRSLPPRQGRVGLLGLL
                     SSLRSEPALDASGGASLAGALARTARLAHSRAVVFVVSDFRGERDWKPALLSLGGRHD
                     VIAVEIRDQREQELPDVGELLLVDPETGRTLRVDTGRRSLRERFAAAAAAERSDLERE
                     LRSVGVDHVVLWTAGDWLQTFAGFLRHRRAMPQGVGRRVSG"
     gene            complement(1418..2422)
                     /locus_tag="VF995_09730"
     CDS             complement(1418..2422)
                     /locus_tag="VF995_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9343879.1"
                     /translation="MAAVGAASATAAHTQLETALFEIKRVIVGQEGMLERVFVALLAG
                     GHLLLEGVPGLAKTLTISTVAKVLGGSFHRVQFTPDLVPSDLVGTRVYRPDSGEFDTE
                     LGPVFCNFLLADEINRAPAKVQSALLEVMQEHQVTIGKTTYPVGDPFLVLATQNPIES
                     EGTYPLPEAQVDRFMMKVLVDYPNFSEELMVVHRSLADQAAVQRALTLEQLHEYQRAT
                     RRIYVDRLVAEYAVALCMATRHPEQYGVSDIAPYIAYGASPRGSINLVHAARALALLR
                     GRSYALPQDVQEVAKDVLRHRLVLTYQALAEEVSADALLTAVLSAITAPRIDLRESTA
                     "
     gene            complement(2902..4599)
                     /locus_tag="VF995_09735"
     CDS             complement(2902..4599)
                     /locus_tag="VF995_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9343880.1"
                     /translation="MSFRARLTLAYLALLTLTLSAFGLGAYAYVDRKLHHELYTSAQN
                     QSNTFALNLTNYETAAAVNSILGLNKLGNQNQTSNPDLYAEVLETPQRPNGRTSQASG
                     QLQVVGKTPSLSQPSVNLPIIPPNHLTVIPARLTGPINRPMVAFAENFVATKPQSGGP
                     RPLVGDQPQPPPPAAKITGRVVVALPLDNVEASLRVLRTILIGGGLAVLATAALLGSG
                     LATVLLRPLARMRTTAQQIGDQRDFTRRMRVEGRPANHHDELARLSLSFNQMLTELEQ
                     AHVDLVAMLEAQRRFVADASHELRTPITAIRTNVEFLSRVPGARPEDRGAALHDVLVE
                     MRRMEALVGDLLALARLEAATSTRPPAEHASEQVRSRPREAVHGRPAKAKAEAEAEAR
                     RSFRLDHLVADVHRDALRHADGKVEVRLGALPEAWVAGDRDDLRRALWNLVDNALKYT
                     PEGWIDLRLEARDGRAALRVADSGVGISEAHLGHVFDRFWRAPSVRGTAGSGLGLAIT
                     RWVAELHGGTVTVESTLGEGTAFTLDLPTSAPSPRRRTRRDGEGEGKRRKQDAFSPL"
     gene            complement(4622..5350)
                     /locus_tag="VF995_09740"
     CDS             complement(4622..5350)
                     /locus_tag="VF995_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9343881.1"
                     /translation="MGAHILVVDDDPILAAALRRPLAYEGFEVEIAASGEEALSRALD
                     HAPDLVILDVLLPGIDGLEVCRRLRQAGDVPVLMLTARAEVPERVAGFEAGADDYLGK
                     PFAFEELLVRVKALLRRGRSAVGATELRFGDLRMDRSTHEVWRGERTISLTRQEFDLL
                     SLFLEHPRQVLTRSTILERVWDYDFGRDSNVLEVYVGYLRRKLEADGEPRVIHTVRGV
                     GYVLRDDPDSVADQGGRMGSGARR"
     gene            complement(5458..7998)
                     /gene="ligD"
                     /locus_tag="VF995_09745"
     CDS             complement(5458..7998)
                     /gene="ligD"
                     /locus_tag="VF995_09745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX9343882.1"
                     /translation="MTPGRRRLRFPEPLPAKRAGEAWRLTVDGRQLRLSNLDKVYWPD
                     EGYTKGDLLAYYYNVAPLLLPHLRDRPETLRRMPDGIAGEAFYEKQVPAHAPDWLPTA
                     PVPHDDPRRTPTDYVLAQDLASLLYVINLGCIELHPLHAKAASLQRPEFALFDLDPFP
                     PYSFADVTAVAKLVKVVLDQLGLRGYPKTSGATGMQIMVPLAPVHPWAEVRAFVERVG
                     RLLVRSWPEKVTMAWPVAERAGKVFVDHPMNRFGANIASVYSLRPLPGAPVSTPLDWE
                     ELDEDLEPGDFRIDNVWGRFAGGDRFAAVLSDRQSLTAALAALGLQPSEATRVEGAAG
                     PAANPEQGPAAGRHGLDEYRRKRDFGRTPEPAPEVGAGPLPEAPHAAGVPEPAQLTPG
                     ARFTIQQHHARALHHDVRFERDGVLVSFAVPKRLPEAPGVKHLAVNTEDHPLDYLAFQ
                     GEIPAGEYGGGSVRLFDLGTYEALEVADGKLSVRLHGSRYHGAEYHFVHTRDRDWICF
                     LAGKAPLPQPAPPPVCEPMLAVPGAQPFDDDGWLFEVKWDGHRCLARLDAAAASDGTR
                     LSSRSGRDVTSQFPELAGLHRQLAGRNAVVDGEVVALDGDGRPSFQRMQDRFHRSADE
                     LARHPGRVPIHFIAFDLLWLDGESLLDLPLSDRRARLAEVLVETREVRLSQAVEGRGQ
                     AFFDEIKALGLEGVVAKRLASPYRPGARSPDWRKVKALRRQDCVIVGWTPGQGERAST
                     LGSLLLAVRDDGDLRYAGNVGTGFTHSFLERLLPELVEREVTAPPLHGVQRLKGLKGV
                     RWARPELVCEVEYLGWTDDGRLRAAAFKGIRTDKLPEDCRREELPAAK"
     gene            8100..8951
                     /locus_tag="VF995_09750"
     CDS             8100..8951
                     /locus_tag="VF995_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX9343883.1"
                     /translation="MRTIWKGAISFGLVTIPIRVFGATEEKSLRFHQLHATDGGRIRY
                     KRVCATDGEEVDYGEIVKGYEYEKDHYVTLTDEELDALPVPTARAIEIERFVDADQID
                     PIYFQKSYYLVPEPAGLKAYQLLREAMADDDKVALAKVAFRDREHLATLRLRDNVFVL
                     ETMYWPDEIRAPKFEELDERVEVRPQEVRMARSLIESLSDDFKPEEFSDAYRAALEDL
                     IAKKIQGEEVTHAPEAEPAKVVDLMEALRASVEAAKSGKPAAGEPAGKPSAKPKAASR
                     RKKAAGE"
     gene            complement(8962..10149)
                     /locus_tag="VF995_09755"
     CDS             complement(8962..10149)
                     /locus_tag="VF995_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX9343884.1"
                     /translation="MATTETQQSQPGRLATPVRRLVKVLLVAFVVIHFVVPQIAGVRR
                     SIHLLEHVNLWLVLLGMCLEAVSIMCYTNLTRAVLPRGDSLPYFTLLRVQLSTLAVSH
                     VVPGGSAAGGALGYRLLTREGLSGTDAAFAQAAQGIGSAVILNLLLWLALLVSIPLHG
                     YNPLYLAAAIIGVVLIGGFAAIVILLMRGERRAAGLVRALARRIPILDEDKAEQVVLR
                     LAARLKTLASDRAMIRRGILWDLGFWLFTAAPLWVFIGAFGHWVRLDGLLIAFGLGYV
                     IAALPITPSGLGLVEGTMIPLLVLFGTDRGIATLGVITYRLVNFWIPIPIGGLAYLSL
                     EAQNERRRAAEGRAAARRRREEELAELAEQSLRDAEDSHRWAERHGLKVPRPSEHESP
                     PAD"
     gene            10317..11006
                     /locus_tag="VF995_09760"
     CDS             10317..11006
                     /locus_tag="VF995_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9343885.1"
                     /translation="MRVLVVEDEADLANAIARGLRREGYAVDVAYDGDEALDKATVNA
                     YDVLCLDLNLPGTDGIEVCRRVRAEQDRGGADPPRVLMLTARDGTRDRVIGLDEGADD
                     YLVKPFDFDELKARIRALLRRDAGRSGAVIQVGDLELDSARHEARRAGRSLPLTPKEF
                     SLLRYFMARAGHVLSQEDLLEHVWDENADPFTNTVRVTVMTLRRKLADAGEGQPIETV
                     VGRGYRLRAEP"
     gene            11050..12231
                     /locus_tag="VF995_09765"
     CDS             11050..12231
                     /locus_tag="VF995_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9343886.1"
                     /translation="MRSIRFRYTLMYSGVLFGLAALVVAALYLILSMSLHGELFGAAQ
                     TFCDAFGHCHQVDVFTGRQFEALVHARTLEKLRTLSFDALVVLFFASFAVGWVIAGRV
                     LAPIGRITAVARDIQATDLSRRIRLPGPEDELKQLADTFDAMLARLDAAFAAQQRFVA
                     DASHELRNPLAIIRTNIDVAMSDPNADPEELRQAIVVVRRASERMARLVDDLLALARR
                     EMPTFEREPVDLGQAVGEASEEFLVPAETRGIVLDRAIAPGVVVIGDRDALKRAVANL
                     LENAVRLGPAGSRIRLAVGSERGRAWVAVSDEGPGIVSDDQAHVFDRFWRADKGRSRA
                     DGGTGLGLAIVRQIAQTHGGEVQLQSRLGVGSTFTIWLPVAELPAEPSPRATSATARR
                     Q"
     gene            12368..13747
                     /locus_tag="VF995_09770"
     CDS             12368..13747
                     /locus_tag="VF995_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9343887.1"
                     /translation="MSTPDAPTPRTTGVPGSGSPTPAVPGPARDADADADAEWVPSRD
                     WTRPDPEAQDDAAWNPAAASPTATTPIPTWTPSEPPATQDPGEPGSAAAGSPPPPDPG
                     QGTGEAPRPSGPGGVGRLGRGIVAAAVVVLLLAGGYGLGQWRSSTHTTSTGGAGIPVT
                     VTPTQIEGSSQEPAVAVAKALLPAVVQIERSDGLGSGVIYDSRGYILTAAHVVDGVNT
                     VVVRLADGTSMQGKVVGTNDATDVGVVKVNRTNLPVAALARNVKLQVGQTAVAIGSPF
                     GLEGSVTAGVVSAISRDLQTSNGTMQVIQTDAPINPGNSGGALADRQGRVIGVNDSIS
                     SQSGGNEGVGFAVPIDTAATSAAQIVSGQPAPTSGFLGVSASDPTLGRAGALISQVVQ
                     GSPAEKAGIQVGDLVVAVNGQAVQSGDALGAQVSALHAGSKAQITLVRNGKETTITAT
                     LASRQGSTG"
     gene            14256..17552
                     /locus_tag="VF995_09775"
     CDS             14256..17552
                     /locus_tag="VF995_09775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX9343888.1"
                     /translation="MTNGSGDLPSAAVASALPDGPPAALAEQLPAGNLVLLGGPGSGK
                     TALLEAAVHTLLARPGGSALYLTSNRPAAVAATERLLDGLAESGGPGSRGGGLRAAGS
                     ALGCVTWYAFARGLVAGHADLLPYRGEPRLLSGPEQWAQVRSLVLEDAAGEQPIDWGE
                     LAPLVGSRAFTDELADFILSCERRLLSPDDLEALAAERHRAAWMPVAAFYRRYLELLA
                     LNDSVDQAGLVTQAGDLLEDEAEVLATLAARTGTVLVDEAQELDRSQLRVLDLLAGAG
                     ARVILAGDPAGTTDEFRGATPGILPGLAARFGARTVLLDRSHRLGGQALDAIHRLQAA
                     APEGAVVMHGGGTTTVQAAGYPGQSEEADAVARQLQLAHQRDGVAYGRMAVLLPSLRR
                     LGGPIRRALDRYGVPYRLVAGERQLVAEPLVRHLLDLFRLALEPDRADELLAPLLTSP
                     LGGLDPRELRALRRAAVLADRSLTDHLAACSDSLDASGGPAAAMGSAPAASGRGTEPP
                     ESTESTESTDPLGVAEPIATRVRALCELLERSRRWVRELEADRCFWEVWQHAPAFAEL
                     VARAAADPTDPATARQLDALTAFSRALVLFVDGRPNATMRTYLDVVERADFASDPWLP
                     PSMARTDAVALLSVNAAKGREFDIVVIAGCVEGALPRTAWPQGLFEAWRLDGDRDPVS
                     HARAIIDAERRRFVLAASRARDRVSFTSAKIDGRGEPSRYLAAFRPASATGAGIAPGT
                     APGTDTSADLGRADPDAAPLGPLEAAGALRRVATDQARPAAERLAAVATLAAMPATTA
                     ATNPATWWWRRDWTEDPDPIATDRRLRTSYSRIGTYEDCHLRYFYSSVAGLDDRTSYQ
                     MAFGKLMHTVFELAAKGEIAEDPTTLKAAYRARFDLAWFPSRVVAQQYWRDGMAMLAL
                     WHDGEAELAKRALAFEVPFEMEVGDHVIRGRIDRVDRVPGGDGEDHGIALLDYKTARI
                     PASDEDARRSLQLAIYYLAALRDPELAALGIPVEMQLVYPARAAQGRFARARQRPGGD
                     HAAEVERRLLPLLDGAAAEQFDPNPHADCHFCAFKPICPMWPQGEDFLAPVIPPAGLD
                     QAGLDAERAE"
     gene            17549..20821
                     /locus_tag="VF995_09780"
     CDS             17549..20821
                     /locus_tag="VF995_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9343889.1"
                     /translation="MSLTVKLNADEATPAIRAALKGWEPTAEQWAAISAPIGPIHVIA
                     GAGSGKTAVMAARIVYLAERLGIEASSVLALTFTNKAAAELEARVGEALAEHPNGSAG
                     IGTEPGSGLGEQVTVHTYHAFAADLVKAYGVRVGVEQDADLLSEAQQYQILLAILDGE
                     RFQHLSVRMAGATIRKALELAAACADHLVEPARVIEASRALIDRADAGEPLPTWQVQA
                     ACERIELAGLVERYTQEKQRRGRIDFGDQVAKAVELVERHQDLVDALRARYPHVLLDE
                     YQDTNVAQRRLIQRLCPPGASIMAVGDARQAIYGFRGATMYNLLSFPEHFPPAGDRTQ
                     AQAALSLSTNFRSGRRILDLANSVIEAIPEERRGGLPLQPAPGAGEGAVLAALLGDQF
                     AEAAFVADQVVRAHAGGLPDGTLPAWRDVAVLVRSKRLLGPLREALEQRDVPVEVVGL
                     QGLLETPEIVDLVSMLRAIEDPGANVPLARLLLGPRWRIGYRHLVRLARWASRHNWGL
                     KGILPGEDPDPGDVSFALAEALDHLDEVDGLDEEARQRLRAFRRELRYLRAAADVPLL
                     DLVETVLERTGVWAELEASSDRRALSARQNLATFLDRVAAFAPVDGDPSLAAFLAYLD
                     AVQDAAEPVEATAPAPADSVKLMTVHMAKGLEFPMVVVAGLSAATAKDGTPKHGIFPD
                     LRVADPRRAQGFPYQLREDAGHLPRFSGNARAFRQQLDQRALEDERRLFYVAVTRAKQ
                     LLVLTAAWWYQGSEKTPKGPSPFWDEAAAHPAATVLARAAQPAASPLAERLRGRIQWP
                     PPARRPEDAADSLFPEGVGAAVELARRDPAAIAARVPPAQVAEFEAALASGRNLQAAA
                     GGEPHLVAPLPPRILSVSQVLLYARCPQDFYWTVVRPLPTQPRVAARLGTVVHRLLER
                     RARGLPDLVDVGDLGGENPAGFAEPGLVERATRNFEASRYARLPVSEAEVGVVLCSGQ
                     WVLRGRIDAVFRTPDGVELVDWKTGRRRQEFTPAGLDQLGIYALALRQLGQLPSEGCT
                     ASYCYLGGDTAEIDPHHYGPAELDEQHRRLEATLAALAAGDFERHCGLAFCPACTRER
                     GG"
     gene            20999..21751
                     /locus_tag="VF995_09785"
     CDS             20999..21751
                     /locus_tag="VF995_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9343890.1"
                     /translation="MPMAEGIREGTQQGTRERILVLLRRHGRLTAPQLADLLSLTSVG
                     VRRHLGLLERDGLVTAETSKPKRGRPAAVYRLTDAGLEKFPRHYDEVAREALAFLKRQ
                     DAAVLSQFLAWRNERLAASYARRVDGATLAERTEALAAVLSEQGFMAEVEPAPDGGGL
                     RLCQHNCTVEHLATELPDLCASEAKLFERLLGSPVQREATIVDGAVRCVTRIHPEARK
                     PNQTSKASMTNKETRKRRVPPAPTPMAARSEA"
     gene            21751..>22796
                     /locus_tag="VF995_09790"
     CDS             21751..>22796
                     /locus_tag="VF995_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX9343891.1"
                     /translation="MATDARDLSQAPERINPVPENVDLGAYKYGWHDPVSSVFTPKRG
                     LSHEVVEEISQMKNEPEWMRKFRHKSLDYFLRRPMPQWGGDLNQIDFNNIYYFLRATE
                     KQAATWEDLPADIKNTYDRLGIPEAEKQRLVSGVAAQYESEVVYHKVREDLEAEGVIF
                     LDTDTALREHEDVFREHFATVIPANDNKFSALNSAVWSGGSYIWIPEGVQVEIPLQAY
                     FRINAQNMGQFERTLIIAEPGSSVHYVEGCLPAGELVAMADGTHRPIETIGVGDDVLS
                     HEGWPVRVKAVQTRDYRGPLYTFVPVSPDNAFRVTAEHPLLVVPRDSVLVKRAPRNGW
                     KPEVETAKLLATTP"
BASE COUNT         3360 a         8574 c         7741 g         3121 t
ORIGIN      
        1 gatcgcgtcc tcgacggcct gccggtcggt ggtgggccgg gcgatcgtct gggcggtgcc
       61 ggagaacgac accaccccga cccggaactt cgcgggcagc tcgttgagga acacggtggc
      121 ggcctgctca gccgcgagca gcctggtcgg ctgcacgtcg gtcgcgttca tcgagccgga
      181 cacgtccatc accagcatga cggtggcctg ctccttggga accagggtga ccgcctgcgg
      241 ccgcgccatc ccgagcagca gcgcggccag cgccagcagg tagagcgcgg gagggagatg
      301 ccggcgccag cgcggcgagt gcgtcaccac gttggcgagc aggtccagat tggtgaaccg
      361 caccgcgtac ctggtacggc gccgctgggc gagcaggtag gcggccagcg ccgccggcag
      421 cagcagcagc gcccacagga acccgggcca cagcacgctc aggtcgctca accgcttacc
      481 ctcctgccga ctccctgcgg catcgcacgc cggtgtcgca ggaaacccgc gaaggtctgc
      541 aaccagtcgc ccgccgtcca cagcacgaca tggtcgacgc cgacgctgcg aagctcgcgc
      601 tccaggtcgc tccgctccgc ggccgccgcc gcggcgaagc gctctctgag gctccgcctg
      661 cccgtgtcga cccgcagggt gcgtccggtc tcggggtcga ccaacagcag ctcgccgaca
      721 tcgggaagct cctgctcccg ctggtcgcgg atctccaccg cgatcacgtc gtggcgcccg
      781 cccagcgaca gcagcgccgg cttccagtca cgttcccccc ggaaatccga gaccacgaac
      841 acgaccgccc tgctgtgcgc cagccgcgcc gtccgagcca gcgcaccggc cagcgacgcc
      901 ccgccggacg cgtccagcgc cggctccgac cgcagcgagg agagcagacc gagcagccca
      961 acccgcccct gccgtggggg gagggagcgg gactcctcgt tgccgaagct gaccagcccg
     1021 agacggttgc cccgccgggt ggcgatgtgc ccgaccgcca gcgcgacgcc ctccgcgacg
     1081 tcgtacttgc gccgctcggc ggtgccgaag ctcatcgacg gggaggcgtc cagcaccagc
     1141 cagctcacca gcgcccgttc ggccacctgg tcgcgcacgt gcggctcgcg cgtccgcgcg
     1201 gtcacgttcc agtcgatccg ccgcacgtcg tcccccggct ggtacaggcg aatctgcgcc
     1261 agctccgtcc cgatccccag ctgactggag cggtactccc cggtcagcag cgcctccacc
     1321 cgccgccgca ccaccacgtc cagcgcccgc agcaacgcct ccgacgtcgg cccaggcccc
     1381 ggccggtcgg gtgtccgctc tggcagccgg gtcgccttca tgcggtggac tcgcgcagat
     1441 cgatccgcgg cgccgtgatc gcggaaagca ccgcggtgag cagggcgtcg gcgctcacct
     1501 cctcggccag cgcctggtag gtcaggacca gccggtggcg caggacgtcc ttggcgacct
     1561 cctgcacgtc ctgggggagc gcgtagctgc ggccccgcag gagggcgagc gcgcgggcgg
     1621 cgtggaccag gttgatgctg ccgcgcgggc tcgcgccgta ggcgatgtag ggggcgatgt
     1681 cgctgacgcc gtactgctcg gggtggcgcg tggccataca gagcgcgacc gcgtactccg
     1741 ccaccagccg gtcgacgtag atccgccggg tggcgcgctg gtactcgtgg agctgctcca
     1801 aggtgagcgc ccgctgtacc gccgcctggt cggcgaggga gcggtgcacc accatcagct
     1861 cctcgctgaa gttcgggtag tcgaccagca ccttcatcat gaagcggtcg acctgggctt
     1921 ccggcagcgg gtaggtgccc tccgactcga tcgggttctg ggtggccagg accaggaacg
     1981 ggtcgcccac cgggtaggtg gtcttgccga tggtgacctg gtgctcctgc atcacctcca
     2041 gcagcgcgga ctgcaccttg gccggggcgc ggttgatctc gtcggcgagc aggaaattgc
     2101 agaacaccgg ccccagctcg gtgtcgaact caccgctgtc gggccggtag acccgcgtgc
     2161 cgaccaggtc ggaggggacc aggtccgggg tgaactggac gcggtggaag ctgccgccga
     2221 gcaccttggc gaccgtggag atcgtcaggg tcttggccag accgggaacg ccctccagca
     2281 gcaggtgccc cccggcgagc agggcgacga agacccgctc cagcatgcct tcctgaccga
     2341 cgatgacgcg cttgatctcg aagagcgcgg tctcaagctg ggtgtgggcc gcggtcgcgc
     2401 tggccgcgcc aaccgcagcc agccgaggct ggtcctgggt caccggtatg gctccttgcc
     2461 gtacggcggc cggtgcccga aggccggcct gctcggggtc gaatcggatc ctgcggtctc
     2521 gacagcgccc actggaacta gattcacgat aacgcgatgc ggaccatcag gcacggtacc
     2581 cgctggtcgc tcgtgtcgat cgcgacggtt ccggtgcgat tgggccggtt ggcggctcag
     2641 ctggcaactt ccccgcctgg cgatgctgga aacccagaga agcgtcccat gggagccaag
     2701 gccccaggaa ggccccaagg ccggagctgt ggctccggcc ttgtcccacc atgtgggcct
     2761 ccggcgtgac gccgggacgg accgagctgg cctcccccgg cctcgcctcc ggatccgctt
     2821 ggctcctgtg ttctacccgg cgaggctgag aacagactga gaatggctta ggacgctctt
     2881 aagaatgcgg ctttatgatc attacaaggg cgagaacgca tcctgtttcc gtcgcttgcc
     2941 ctcgccctca ccatcgcgcc gggtgcgccg ccggggcgag ggggcgctgg tcggcaggtc
     3001 gagggtgaag gcggtcccct ccccgagggt cgactcgacc gtgaccgtgc cgccgtgcag
     3061 ctcggcgacc cagcgggtga tcgccaaccc cagcccggag ccggcggtgc cgcgcacgct
     3121 cggagcgcgc cagaagcggt cgaacacatg gccgaggtgc gcctcgctga tcccgacgcc
     3181 gctgtcggcg acgcggagtg ccgcgcggcc gtcgcgcgcc tccagccgca ggtcgatcca
     3241 cccctccggg gtgtacttga gggcgttgtc gaccaggttc cacagcgccc ggcgcaggtc
     3301 gtcgcggtcg ccggcgaccc acgcctcggg cagcgcgccg agccgcacct ccaccttgcc
     3361 gtccgcgtgg cgcagcgcgt cccggtgcac gtcggcgacc aggtggtcga ggcggaagct
     3421 gcgccgcgcc tccgcctccg cctccgcctt cgccttcgcc ggcctcccgt ggacggcctc
     3481 ccgtggacgg cttcgcactt gctcgcttgc atgctcggcc ggcggccggg tgctggtggc
     3541 cgcctccagc cgcgccagcg ccagcaggtc gccgaccagg gcctccatcc gccgcatctc
     3601 gaccagcacg tcgtgcaggg ccgcaccccg gtcctcgggg cgcgcgccgg ggacccggct
     3661 caggaactcg acgttggtgc ggatcgcggt gatcggcgtg cgcagctcgt gcgaggcgtc
     3721 ggcgacgaac cgccgctgcg cctccagcat cgccaccagg tcgacgtggg cctgctccag
     3781 ctcggtcagc atctggttga acgacagcga caggcgggcc agctcgtcat ggtggttggc
     3841 gggccggccc tcgacccgca tgcggcgggt gaagtcgcgc tggtcgccga tctgctgcgc
     3901 ggtggtccgc atccgtgcca gcggccgcag cagcaccgtg gccaggccgg aaccgagcag
     3961 cgccgcggtc gccagcaccg cgaggccgcc gccgatcagg atcgtgcgca ggacccgcag
     4021 cgacgcctcc acgttgtcga gcggcagcgc gaccaccacc cggccggtga tcttggcggc
     4081 cgggggcggg ggctggggct ggtcccccac gagcggccgc ggcccgccgg actgcggctt
     4141 ggtcgcgacg aagttctcgg cgaaggccac catcgggcgg ttgatcgggc cggtgaggcg
     4201 tgccgggatc accgtgaggt ggttcggcgg gatgatcggc aggttgacac tgggctgcga
     4261 aagcgacggc gttttgccga cgacctgcag ctggccgctc gcctggctgg tcctgccgtt
     4321 tggccgttgc ggagtctcca ggacctcggc gtagagatct gggttcgacg tctggttctg
     4381 gttgccgagc ttgttcaggc cgaggatgct gttgacggcc gcggcagtct cataattggt
     4441 gagattcagc gcgaaggtgt tgctctggtt ctgcgccgag gtatacagct cgtggtggag
     4501 cttgcggtcc acgtaggcgt acgcccccag cccgaaggcg ctcagcgtca gggtcagcag
     4561 cgccaggtag gccagggtca gccgcgcccg gaagctcatc ggtccgcacc cgcacgcccg
     4621 gtcatcgtcg tgcgccgctt cccatgcgcc caccctggtc cgcaaccgaa tccgggtcgt
     4681 cgcgaagcac gtagccgacg ccgcggacgg tgtggatcac ccgcggctcg ccgtcagcct
     4741 cgagctttcg gcgcaggtag ccgacgtaga cctccagcac gttggagtcg cggccgaagt
     4801 cgtagtccca gacccgctcc aggatggtcg agcgggtcag cacctggcgg ggatgctcca
     4861 ggaacagcga cagcaggtcg aactcctgcc gggtgagcga gatggtgcgc tcaccgcgcc
     4921 agacctcgtg ggtggagcgg tccatgcgca ggtcgccgaa gcgcagctcg gtcgcgccta
     4981 cggcgctgcg tccgcggcgc agcagcgcct tcacccggac cagcagctcc tcgaaggcga
     5041 acggcttgcc gaggtagtcg tcggcgcccg cctcgaagcc ggcgacccgc tcgggcacct
     5101 cggccctcgc ggtgagcatc agcaccggga cgtccccggc ctgacgaagc cgccggcaca
     5161 cctccagccc gtcgatcccg ggcagcagca catcgaggat caccaggtca ggggcatggt
     5221 cgagggcgcg gctcagggcc tcctcaccgg aggcggcgat ctccacctcg aagccttcgt
     5281 aggcgagcgg ccgccgcagc gccgcagcga ggatgggatc gtcgtccacg acgagaatgt
     5341 gggcacccat acgcatgtcg cccttcgttg ccgccttacc tgaaaactcc ggagcctgcg
     5401 cccctgaaat tctgcctgct caaaccggtt cttgcctagc gaagccagct gacagcgcta
     5461 tttcgcggcg gggagttcct cacggcggca gtcttcgggg agtttatcgg tcctgatccc
     5521 cttgaacgcg gccgcccgca ggcgaccgtc gtcggtccag ccaaggtatt cgacttcaca
     5581 gaccagctcg ggccgggccc agcgtacccc tttcaggcct ttcaggcgct ggacgccgtg
     5641 cagcggcggc gccgtgacct cccgctcgac cagctcgggc agaagccgtt ccagaaacga
     5701 gtgggtgaaa ccggtcccga cattcccggc ataccgcagg tcgccgtcgt cccgaaccgc
     5761 cagcagcagc gagccgaggg tcgaagcgcg ctcgccctgg ccgggcgtcc agccgacgat
     5821 cacgcagtcc tggcggcgca gggccttgac cttgcgccag tccggcgagc gcgcccccgg
     5881 gcggtagggg ctcgccaggc gtttggccac cacgccctcc agcccgagcg ccttgatctc
     5941 gtcgaagaac gcctggccgc gcccctcgac cgcctgggag aggcgtacct cgcgggtctc
     6001 gaccagcacc tcggccaggc gggcgcgccg gtcggacagg ggcaggtcga gcagcgactc
     6061 gccgtccagc cagagcaggt cgaaggcgat gaagtggatc ggcacccggc cgggatggcg
     6121 ggccagctcg tcggcggagc ggtggaagcg gtcctgcatg cgctggaagg agggccgccc
     6181 gtcgccgtcc agggcgacga cctcgccgtc gaccaccgcg ttgcggccgg ccagctggcg
     6241 atgcagcccg gccagctcgg ggaactggct ggtcacgtcg cgcccgctcc gggacgagag
     6301 gcgggtcccg tcgctggcgg ctgccgcgtc cagccgcgcc aggcagcggt gcccgtccca
     6361 tttgacctcg aacagccagc cgtcgtcgtc gaacggctgc gcgccgggca cggccagcat
     6421 cggctcgcac actggcggcg gcgcgggctg ggggaggggg gccttcccgg cgaggaagca
     6481 gatccagtca cggtctctgg tgtggacgaa gtggtattcg gcgccgtggt agcggctgcc
     6541 atgcaggcgc acgctcagct tgccgtcggc gacctccagc gcctcgtagg tgcccaggtc
     6601 gaacaggcgc acgctgccgc cgccgtactc gcccgcgggg atctcgccct ggaaggccag
     6661 gtagtccagc gggtggtcct cggtgttgac ggccaggtgc ttgaccccgg gcgcctccgg
     6721 cagccgcttc gggaccgcga aggagaccag caccccgtcg cgttcgaagc ggacgtcatg
     6781 gtggagggcg cgggcgtggt gctgctggat ggtgaaccgc gcccccgggg tgagctgggc
     6841 cggctccggg acccccgccg cgtgcggcgc ctccggcagc gggcccgccc ccacctccgg
     6901 agcaggttcc ggggtccggc cgaagtcgcg cttgcgacgg tactcgtcga ggccatggcg
     6961 tcctgccgcg gggccctgct cggggttcgc cgctggtccc gccgcgccct ccaccctggt
     7021 cgcctcgctc ggttgcaggc caagcgccgc cagcgcggcg gtgagcgact ggcggtcgga
     7081 cagcacggcg gcgaagcggt cgccgcccgc gaagcgcccc cagacgttgt cgatgcgaaa
     7141 gtcgcccggc tcaaggtcct cgtccagttc ctcccagtcc agcggagttg acactggcgc
     7201 ccccggcagc ggccgcagcg agtacacgct ggcgatgttg gcgccgaacc ggttcatcgg
     7261 gtggtccacg aagaccttcc cggcccgctc cgccaccggc cacgccatgg tcaccttctc
     7321 cggccagctt cggaccagca ggcggccgac ccgctcgacg aacgcacgca cctccgccca
     7381 cggatgcacc ggcgccagcg gcaccatgat ctgcatcccg gtggcacccg aggtcttggg
     7441 gtacccgcgc aggccgagct ggtcgagcac caccttgacc agcttggcga ccgcggtcac
     7501 gtcggcaaag ctgtagggcg ggaatgggtc caggtcgaac agggcgaact cgggccgctg
     7561 cagcgaggcg gccttggcgt gtagcgggtg cagctcgata catcccaggt tgatcacata
     7621 cagcaggctc gccaggtcct gcgccagcac gtagtccgtg ggcgtccgac gtggatcatc
     7681 gtgggggact ggcgcggtcg gcagccagtc gggggcatgc gctgggacct gcttctcgta
     7741 gaacgcctcg ccggcgattc cgtccggcat gcgccgcagc gtctcgggcc ggtcccgcag
     7801 gtgcggaagc agcaggggcg ccacgttgta gtagtaggcg agcaggtcgc ccttggtgta
     7861 gccctcgtcc ggccagtaga ccttgtcgag attggagaga cggagctggc gcccgtccac
     7921 cgtcagccgc caggcctcgc cggcccgttt ggcgggcagg ggctctggga agcgcagacg
     7981 gcgtcgacca ggggtcacgt gtgtcaactc cttctgctct gggtcgtcgc cagcgtaccc
     8041 tgagtacctg ggcaagcccg ccgcacggag ccgccgcctg gatcaggagc acagcgcgaa
     8101 tgaggacgat ctggaagggc gcgatctcct tcggcctggt caccatcccg atccgggtct
     8161 tcggggcaac cgaggagaag tccctccgct tccaccagct ccacgccacc gacggtggcc
     8221 ggatccgcta caagcgggtc tgcgccaccg acggcgagga ggtcgactac ggcgagatcg
     8281 tcaagggcta cgagtacgag aaggaccatt acgtcaccct gaccgacgag gagctggacg
     8341 cgctgccggt gccgaccgcc cgcgcgatcg agatcgaacg gttcgtcgac gccgaccaga
     8401 tcgacccgat ctacttccag aagtcctatt acctggtgcc cgagccggcc ggcctcaagg
     8461 cctaccagct gctgcgcgag gccatggccg acgacgacaa ggtggcgctc gccaaggtcg
     8521 ccttccgcga ccgggagcac ctggccaccc tgcgcctgcg cgacaacgtc ttcgtgctgg
     8581 agacgatgta ctggcccgac gagatccgcg ccccgaagtt cgaggagctg gacgagcggg
     8641 tcgaggtgcg gccgcaggag gtccgcatgg cccgcagcct gatcgagagc ctctccgacg
     8701 acttcaagcc ggaggagttc agcgacgcct accgcgccgc cctggaggac ctgatcgcca
     8761 agaagatcca gggcgaggag gtcacccacg cccccgaggc cgagcccgcc aaggtcgtcg
     8821 acctgatgga ggccctgcgc gccagcgtcg aagccgcaaa gtcaggcaaa ccggctgcgg
     8881 gtgagccggc agggaagcca tccgccaagc cgaaggccgc ctcccgccgc aagaaggcgg
     8941 cgggggagta ggcccagcgc gctaatcggc tggcggggac tcgtgctcgc tggggcgggg
     9001 gactttgagg ccgtggcgtt cggcccaccg gtgggagtcc tcggcgtcgc gcagggactg
     9061 ctcggccagc tcggccagct cctcctcgcg ccgccgccgc gccgccgcgc gcccctccgc
     9121 cgcccggcgg cgctcgttct gcgcctccag cgacaggtag gcgagcccgc cgatcgggat
     9181 cgggatccag aagttcacca gccggtaggt gatcactccg agggtggcga tgccccggtc
     9241 ggtgccgaac agcaccagca gcgggatcat cgtgccctcg accaggccaa ggccggacgg
     9301 ggtgatcggc agcgccgcga tcacgtagcc gaggccgaag gcgatcagca ggccgtccag
     9361 ccgcacccag tgcccgaacg cgccgatgaa cacccacagc ggcgcggcgg tgaacagcca
     9421 gaagccgagg tcccagagga tcccccggcg gatcatggcg cggtcgctcg ccagcgtctt
     9481 gaggcgggcg gccaggcgca gcacgacctg ctcggcttta tcctcgtcga ggatggggat
     9541 ccggcgggca agcgcccgca ccaggccggc ggcgcggcgc tcgcctcgca tcagcaggat
     9601 cacaattgcc gcgaagccac cgatcaggac cacaccgatg atcgcggcgg ccaggtagag
     9661 ggggttgtag ccgtgcagtg ggatcgagac cagcagtgcc agccacagca gcaggttcag
     9721 gatcaccgcc gagccgatgc cctgcgcggc ctgggcgaag gcggcgtcgg tccccgacag
     9781 cccctccctg gtgagcagac ggtacccgag cgcgccgcca gcggccgagc cgcccgggac
     9841 cacgtggctg acggccaggg tcgagagctg gacgcgcagc agggtgaagt acggcagcga
     9901 gtcgccgcgc ggcagcaccg ccctggtcag gttcgtgtag cacatgatcg agacggcctc
     9961 gaggcacatc ccgagcagca ccagccacag gttcacgtgc tcaagcaggt ggatcgagcg
    10021 gcgcaccccg gcgatctgcg gcaccacgaa gtggatgacc acaaatgcga ccagcaacac
    10081 ctttaccagc cgtctgaccg gcgtggccag ccggcccggc tgggactgct gcgtctcggt
    10141 agtagccacc cccgctaccg cacgtcgacc tggacgggac acaaccgcat ggtggggcca
    10201 agtataggtg cgcttcaggg acttcgtgcc ggcggatctc accggctcac gcccgggtaa
    10261 ccttcccggt ggcgtcttga cgcctgcttg atcgattcgg cggttaccat ttgcacatgc
    10321 gtgtgttggt ggtggaggac gaggccgacc tggccaacgc gatcgctcgg ggcctgcgcc
    10381 gcgagggcta cgcggtcgac gtcgcctacg acggcgacga ggcgctcgac aaggcgacgg
    10441 tgaacgccta tgacgtgctg tgcctcgacc tcaacctgcc cggcaccgat ggcatcgagg
    10501 tctgccgccg cgtccgggcg gagcaggacc gcggcggcgc cgatccgccg cgggtgctga
    10561 tgctcaccgc ccgcgacggc accagggacc gggtcatcgg cctcgacgaa ggggccgacg
    10621 actacctggt caagccgttc gacttcgacg agctgaaggc gcggatccgg gcgctgctgc
    10681 ggcgcgacgc ggggcgctcc ggcgcagtca tccaggtcgg cgacctggag ctggacagcg
    10741 ccaggcacga agcccgccgt gccggccgct cgctcccgct caccccaaag gagttctctc
    10801 tgctgcgcta cttcatggcg cgcgccggtc acgtcctgtc ccaggaggac ctgcttgagc
    10861 acgtctggga cgagaacgcc gaccccttca ccaacaccgt ccgcgtcacg gtgatgaccc
    10921 tgcggcgcaa gctcgccgac gccggcgagg gccagccgat cgagaccgtg gtcggtcgcg
    10981 gctaccggct gcgcgcggaa ccctgacgtg aaggcgccgg agcgacaggc ggagcgactg
    11041 ccggagtgga tgcggtcgat ccgcttccgc tacaccctga tgtactcggg ggtcctgttc
    11101 ggcttggccg cgctggtggt ggccgcgctg tacctgatcc tgtcgatgag cctccatggc
    11161 gagctgttcg gcgcggcgca gacgttctgc gacgcgttcg gccactgcca ccaggtcgac
    11221 gtcttcaccg gccggcagtt cgaggcgctg gtccatgccc gcaccctgga gaagctgcgc
    11281 accctgtcat ttgacgcgct ggtggtgctg ttcttcgcca gcttcgcggt cggctgggtg
    11341 atcgccgggc gggtcctcgc cccgatcggg cggatcaccg cggtcgcccg cgacatccag
    11401 gccaccgacc tgtcgcgccg catccgcctg cctggccccg aggacgagct gaagcagctc
    11461 gccgacacct tcgacgcgat gctcgcccgc ctcgacgccg ccttcgcggc gcagcagcgg
    11521 ttcgtcgccg acgcctccca cgagctgcgc aacccgctgg cgatcatccg caccaacatc
    11581 gacgtcgcca tgtccgaccc gaacgccgac ccggaggagc tgcgccaggc gatcgtggtg
    11641 gtgcgccgcg ccagcgagcg catggccagg ctggtcgacg atctgctggc gctggcgcgg
    11701 cgcgagatgc ccaccttcga gcgtgagccg gtcgatctcg gccaggcggt cggcgaggcg
    11761 agcgaggagt tcctggtccc cgccgagacc cgggggatcg tgctcgaccg ggccatcgcc
    11821 cccggggtgg tcgtgatcgg cgaccgggac gcgctcaagc gggcggtcgc caatttgctt
    11881 gagaacgccg tccgcctcgg ccccgccggc tcgcggatcc gcttggcggt cggcagcgag
    11941 cggggccgtg cctgggtcgc ggtgtccgat gagggcccgg ggatcgtatc cgacgaccag
    12001 gcgcacgtgt tcgaccgctt ctggcgtgcg gacaaaggcc gctcccgggc tgacggcggc
    12061 accggcctcg gccttgcgat cgtgcgccag atcgcccaga cccacggcgg cgaggtgcag
    12121 ctccagtccc gcctgggcgt cggctcgacc ttcacgatct ggctgccggt cgccgagctt
    12181 ccggcggagc cgtcgccccg cgccacctcc gcgaccgctc gccggcagtg accgtgaccg
    12241 ctgcgcgagc gggcgcgaca ttttatatga cgttgactgg tgccttaccg atgttttagc
    12301 ggccaccctt tatgttccaa cgcggaactc cgtaggaagc tgagcgaacc caggaggcaa
    12361 cacccagatg tccacccccg acgcaccaac cccacgcacg actggcgtgc ctggctccgg
    12421 ttcgccgaca cccgcggtcc cgggtcccgc tcgcgacgcc gacgccgacg ccgacgccga
    12481 atgggtcccg tcgcgcgact ggacccggcc tgaccccgag gcgcaggacg acgcggcctg
    12541 gaacccggcc gccgcgtcgc ccaccgccac caccccgatc ccgacctgga cgccgtccga
    12601 gccgcccgcg acccaggatc cgggcgagcc gggctcggcc gcggcaggct cgccgccgcc
    12661 tcccgacccc ggccagggga cgggcgaagc tccgcgcccc agcggtcccg gcggcgtcgg
    12721 gcggctcggc cgcgggatcg tcgccgccgc cgtggtggtc ctgctgctcg ccggcggcta
    12781 cggcctcggc cagtggcgca gttcgacgca taccaccagc accggcggcg ccggcatccc
    12841 ggtcacggtg acaccgaccc agatcgaggg cagcagccag gagccggcgg tggcggtcgc
    12901 caaggcgctg ctgccggcgg tcgtgcagat cgagcgctcc gacggtctcg gctcgggcgt
    12961 gatctacgac tcgcgcggct acatcctcac cgccgcgcac gtggtcgacg gggtcaacac
    13021 ggtcgtcgtg cgcctcgccg acggcacctc catgcagggc aaggtggtcg gcaccaacga
    13081 cgccaccgac gtcggtgtgg tcaaggtcaa ccgcaccaac ctcccggtgg ccgccctcgc
    13141 ccgcaacgtc aagctccagg tggggcagac ggccgttgcg atcggcagcc cgttcgggct
    13201 ggagggctcg gtgaccgccg gcgtggtcag cgccatcagc cgcgacctgc agacctccaa
    13261 cggcaccatg caggtcatcc agaccgacgc ccccatcaat ccgggcaact ccggcggcgc
    13321 cctcgccgac cggcagggcc gcgtgatcgg cgtcaacgac tcgatcagct cgcagtccgg
    13381 cggcaacgag ggcgtcgggt tcgcggtgcc gatcgatacc gccgccacct cggccgccca
    13441 gatcgtcagc ggtcagcccg cgccgaccag cggcttcctc ggtgtcagcg ccagcgatcc
    13501 caccctcggc cgcgctggcg cgctgatcag ccaggtcgtg cagggcagcc ccgccgagaa
    13561 ggccggcatc caggtcggag acctggtcgt ggccgtcaat ggccaggcgg tgcagagcgg
    13621 ggacgcgctc ggcgcccagg tcagcgcctt gcacgcgggc tcgaaggccc agatcacact
    13681 ggtccgcaac ggcaaggaga cgaccatcac cgcgacgctt gcatcccggc aaggaagcac
    13741 aggatgatca tctacgcatc cctagctcaa cctgactaga cacccccgac cgaatctggt
    13801 agtttggctg gtaacgattt ggaatcaagc aaagcacgaa ccggaacggg catcgtaagt
    13861 tagtgacgcg aacacggaac tggtcttgag cggcgctggg gggcgctcgt aagggggcca
    13921 tttccgcccg catgacggcc ggggccgtcg tgccttggcc gggaggacac gggaggcgcg
    13981 ggaagcaggt tcgcgttatg gcggcctgcc gtccggcagt gaggaccggt ggttggggga
    14041 ctgccgggac cccgccggac ggcaggccgt ctttttcatg ctcgccttct cgccgcctga
    14101 atcgcaacgg gagccattga gattcgggct ggcccaaata gcctggcctt tagcctggcc
    14161 ttccctgcgc agccgctggc ctgaacgccg ttcgccgccg gccgcctcgt ggataacccc
    14221 ggcagaccgt cacggggagt gtttaggatt cgggcatgac gaacggatca ggtgacctcc
    14281 ccagcgcggc cgtggccagc gccctaccag acggcccgcc agccgctctg gccgaacagc
    14341 tccccgccgg gaacctggtc ctgctcggcg gccccggaag cggcaagacg gccctgctgg
    14401 aggcagccgt ccacaccctg ctcgcccgtc caggcgggag cgcgctctac ctcacctcga
    14461 accgccccgc cgcggtcgcc gccaccgagc ggctgctcga cggcctcgcc gaaagcggcg
    14521 gccctggaag caggggcggc ggcctgcggg ccgctgggag cgccctgggc tgcgtcacct
    14581 ggtacgcctt cgcccgcggg ctggtcgccg gccacgccga cctgctgccc taccgcggcg
    14641 agccgcgcct gctcagcggc ccggaacagt gggcccaggt gcgctccctg gtgctcgaag
    14701 acgccgcggg cgagcagcca atcgactggg gcgagctggc accgctggtc ggctctcgcg
    14761 cgttcaccga cgagctggcc gacttcatcc tgtcctgcga gcgccgcctg ctcagccccg
    14821 acgacctgga ggcactcgcc gccgagcggc accgcgcggc ctggatgccg gtggcggcgt
    14881 tctaccgccg ctacctcgaa ctgctcgccc tcaacgactc ggtcgaccag gccggcctgg
    14941 tgacgcaagc cggcgacctg ctggaagacg aggccgaggt gctcgccacc ctggccgccc
    15001 ggaccggcac ggtcctggtc gacgaggcac aggagctcga ccgctcccag ctccgcgtgc
    15061 tcgacctgct ggctggcgcc ggcgcccggg tgatcctcgc cggcgaccca gcgggcacca
    15121 ccgacgagtt ccgcggcgcc accccaggca tcctgccggg cctcgctgcc cgcttcggcg
    15181 cccggacggt cctgctcgac cgcagccacc gcctcggcgg ccaggcgctc gacgccatcc
    15241 accgtctcca agccgcggct cccgaggggg cggtggtgat gcacggtggc ggcaccacca
    15301 cagtccaggc cgccgggtat cccgggcaat ccgaggaggc ggacgcggtg gcgcgccagc
    15361 tccagctcgc gcaccaacgc gacggggtcg cctacggccg catggcagtc ctgctgccgt
    15421 cgctgcgccg cctcggcggc ccgatccgcc gcgcactcga ccgctacggc gtcccctacc
    15481 ggctcgtcgc cggcgaacgc cagctcgtcg ccgagccgct cgtccgccac ctgctcgacc
    15541 tgttccgcct cgccctggaa cccgaccgcg ccgacgagct gctggccccg ctgctcacct
    15601 cccccctcgg cggcctcgac ccccgcgagc tgcgggccct ccgccgcgcc gccgtgctgg
    15661 ccgaccgctc cctcaccgac cacctcgccg cctgttcaga ttcccttgac gcatcggggg
    15721 gtcccgcggc tgccatggga tcggcccccg ctgcttccgg ccgcggtacc gaaccccccg
    15781 aatccactga atccaccgaa tccaccgatc cgctcggggt cgccgagccg atcgccacgc
    15841 gggtccgggc cctctgcgag ctgctggagc ggagccgccg ctgggtccgc gaactcgagg
    15901 ccgaccgctg cttctgggag gtctggcagc acgcgcccgc cttcgccgag ctggtcgccc
    15961 gcgccgccgc cgaccccacc gacccggcca ccgccaggca gctcgacgca ctcaccgcgt
    16021 tctctcgcgc gctcgtcctg ttcgtcgacg gccgacccaa cgcgaccatg cgcacctacc
    16081 tggacgtggt cgaacgggcc gacttcgcca gcgatccctg gctgcccccc tcgatggccc
    16141 ggaccgacgc ggtcgcgctg ctgtccgtca acgccgccaa gggccgggag ttcgacatcg
    16201 tggtcatcgc cggctgtgtc gagggcgccc tgccccgcac cgcctggccg cagggcctgt
    16261 tcgaggcctg gcggctcgac ggcgaccggg accctgtgag ccacgcccgc gcgatcatcg
    16321 acgccgagcg ccgccgcttc gtcctggccg ccagccgcgc cagggaccgg gtcagcttca
    16381 cctccgccaa gatcgacggc cgtggtgagc cgtcccgcta cctggccgcc ttccgccccg
    16441 cctccgcaac tggcgccgga atcgcccccg ggaccgcccc cgggaccgat accagcgccg
    16501 acctcggccg cgccgatccc gacgcggcgc cgctcggccc gctggaggcg gccggcgcgc
    16561 tgcggcgggt cgccaccgac caggcacgcc cagcggccga gcggctcgcc gccgtcgcga
    16621 ccttggccgc catgccggcc accaccgccg cgaccaaccc cgcgacctgg tggtggcggc
    16681 gcgactggac cgaggacccc gacccgatcg ccaccgaccg gcgcctgcgc acctcctact
    16741 cgcgcatcgg cacctacgag gactgccacc tgcgctactt ctactccagc gtcgccgggc
    16801 tcgacgaccg cacctcctac cagatggcct tcggcaagct gatgcacact gtcttcgagc
    16861 tcgccgccaa aggcgagatc gccgaggacc ccaccacgct caaggccgcc taccgcgccc
    16921 gcttcgacct ggcctggttc ccctcccggg tggtcgccca gcagtactgg cgtgacggca
    16981 tggccatgct ggcgctgtgg cacgacggcg aggccgaact cgccaagcgc gccctcgcct
    17041 tcgaggtccc cttcgagatg gaggtcggcg accacgtgat ccgcggccgc atcgaccggg
    17101 tggaccgcgt ccccggaggc gacggggagg accacggcat cgccctgctc gactacaaga
    17161 ccgcccgtat ccccgcctcc gacgaggacg cccgccgctc gctgcagctc gccatctact
    17221 acctggccgc cttgcgggac ccggagctgg ccgcgctcgg gatccccgtc gagatgcagc
    17281 tggtctaccc ggcacgagcc gcccagggcc gcttcgcgcg cgcccgccag cggccagggg
    17341 gcgaccacgc cgccgaggtc gaacggcgcc tgctgccact gcttgacggc gccgctgccg
    17401 agcagttcga cccgaacccc cacgctgact gccacttctg cgctttcaaa ccgatctgcc
    17461 cgatgtggcc ccagggtgag gacttcctcg ccccagtgat ccccccagcc ggcctcgacc
    17521 aagccggcct cgacgcggag cgtgccgaat gagcctcacc gtcaagctga acgcggacga
    17581 ggcgaccccg gcgatccggg cagccctcaa aggctgggag ccgaccgccg agcagtgggc
    17641 cgcgatcagc gcccccatcg gacccatcca cgtgatcgcc ggagccgggt cgggcaagac
    17701 cgcggtcatg gccgcccgca tcgtctacct ggccgaacga cttgggatcg aggcctcgtc
    17761 ggtgctcgcg ctcaccttca ccaacaaggc cgccgccgaa ctggaggcgc gcgtcggcga
    17821 ggcactcgcc gaacacccca acggcagcgc cggaatcgga accgagccag ggtccgggct
    17881 gggcgagcag gtcacggtgc acacctacca cgccttcgcc gccgacctgg tcaaggccta
    17941 cggcgtccgc gtcggcgtcg agcaggacgc cgacctgctc agcgaggcgc agcagtacca
    18001 gatcctgctc gcgatcctcg acggcgagcg cttccagcac ctcagcgtgc gcatggccgg
    18061 cgccaccatc cgcaaggcgc tggagctcgc cgccgcctgc gccgaccacc tggtcgagcc
    18121 cgccagggtc atcgaggcgt cccgcgccct gatcgaccgg gccgacgccg gcgagccgct
    18181 gcccacctgg caggtccagg ctgcctgcga acggatcgag ctggccggcc tggtcgagcg
    18241 ctacacccag gagaagcagc gccgcggccg catcgacttc ggcgaccagg tcgccaaggc
    18301 ggtcgagctg gtcgagcggc accaggacct ggtcgacgcg ctgcgcgccc gctaccccca
    18361 cgtgctgctc gacgagtacc aggacaccaa cgtcgcccag cgccgcctga tccagcggct
    18421 gtgcccccct ggcgcctcga tcatggcggt cggcgacgcc cgccaggcga tctacggctt
    18481 ccgcggcgcg accatgtaca acctgctctc gttccccgag cacttcccac ccgccggcga
    18541 tcgcacccag gcccaggcgg cgctgtcgct gtccaccaac ttccgttccg ggcgccgcat
    18601 cctcgacctc gccaactccg tgatcgaggc catccccgag gaacgccgcg gcggcctgcc
    18661 cctgcagccg gctcccggcg ccggcgaggg cgcggtgctc gccgccctgc tcggcgacca
    18721 gttcgccgag gccgccttcg tcgccgacca ggtcgtccgg gcccacgccg gcggcctgcc
    18781 cgacggcacc ctgcccgcct ggcgcgacgt cgccgtgctg gtgcgctcca aacggctgct
    18841 cggccccctc cgcgaggcgc tcgagcaacg cgacgtcccg gtcgaggtcg tcggcctgca
    18901 gggcctgctg gaaacccccg agatcgtcga cctggtcagc atgctgcggg cgatcgagga
    18961 cccgggtgcc aacgtgccgc tcgcccggct gctgctcggc ccccgctggc gcatcggcta
    19021 ccgccacctg gtccgcctcg cccgctgggc ctcccggcac aactggggcc tcaagggcat
    19081 cctccccggc gaggaccccg accccgggga cgtgtccttc gcgctggccg aggcgctcga
    19141 ccacctcgac gaggtcgacg gcctcgacga ggaggcacgc cagcggctgc gcgcctttcg
    19201 ccgggagctg cgctatctgc gcgccgccgc ggacgtcccc ctgctcgacc tggtcgagac
    19261 cgtgctggaa cgtaccggcg tctgggccga gctggaggcc tccagcgacc gccgcgcgct
    19321 ctccgcccgc cagaacctcg ccaccttcct cgaccgggtg gccgctttcg cgccggtcga
    19381 cggcgacccg tcgctggccg cgttcctcgc ctacctcgac gcggtccagg acgccgccga
    19441 accggtcgag gcgaccgccc ccgcccccgc cgactcggtc aagctcatga ccgtccacat
    19501 ggccaagggc ctggaattcc ccatggtggt tgtcgccggc ctgtccgccg ccaccgccaa
    19561 ggacggcacc cccaaacacg gcatcttccc cgacctccgg gtcgccgacc cgcgccgcgc
    19621 ccagggcttc ccctaccagc tgcgcgagga cgccggccac ctgccccgct tcagcggcaa
    19681 cgcccgcgcc ttccgccagc agctcgacca gcgcgcgctg gaggacgaac gccgcctgtt
    19741 ctacgtcgcc gtcacccgcg ccaaacaact cctcgtcctc accgccgcct ggtggtacca
    19801 ggggtcggag aagaccccca agggaccgag cccgttctgg gacgaagccg ccgcccaccc
    19861 ggccgccacc gtcctcgccc gcgccgccca gccggccgcc agcccgctcg ccgagcgcct
    19921 ccgcggccgc atccaatggc cacccccggc gcgccgccca gaagacgccg ccgactcgct
    19981 gttccccgaa ggggtgggcg ccgccgtgga actggcccgc cgcgaccccg ccgccatcgc
    20041 cgcccgcgtg ccgcccgccc aggtcgcaga gttcgaagcc gccctggcct ccgggcgcaa
    20101 cctccaggcc gccgccggag gcgagccgca cctggtcgcc ccgctgcccc cgcggatcct
    20161 ctcggtcagc caggtgctcc tctacgcccg ctgcccccag gacttctact ggacggtagt
    20221 gcgcccgctg ccgacccagc cgcgcgtggc agcccggctc gggaccgtcg tgcaccgcct
    20281 gctggaacgt cgcgcccgcg gcctccccga cctggtcgac gtcggcgacc tcggcggcga
    20341 aaaccccgcc ggcttcgccg agccaggact ggtcgagcgg gccacccgca acttcgaagc
    20401 ctcccgctac gctcgcctgc ccgtctccga agccgaggtc ggcgtggtcc tgtgctccgg
    20461 ccagtgggtc ctgcgcggcc gcatcgacgc ggtcttccgc acccccgacg gcgtcgagct
    20521 ggtcgactgg aagaccgggc gccggcgcca agaattcacc ccggccgggc tcgaccagct
    20581 cggcatctac gcgctcgccc tgcgccagct cggccagctc ccctccgagg gctgcaccgc
    20641 ctcctactgc taccttggcg gcgacacggc cgagatcgat ccgcaccact acggcccggc
    20701 cgaactcgac gagcagcacc ggcggctgga agccaccctg gcggcactcg ccgccggcga
    20761 cttcgagcgc cactgcggcc tggccttctg cccagcctgc acccgcgagc gcggcggctg
    20821 agcgctggtc gacgagcccg acccgctccg cagcggacgg ggggatttgc ctgtccgcag
    20881 ggaggccggg taacctggca caaggaaaca tctatgtttc caaagtcacc ttcggggcag
    20941 gcgggacgcc aggaccgcgg cactcgaagc gccacagaag caccaggaag gacgcgtcat
    21001 gccaatggcc gaggggatcc gagagggcac ccagcagggc acccgggaac gcatcctcgt
    21061 cctgctgcgc cgccacgggc gcctgaccgc gccgcagctc gccgacctgc tcagcctcac
    21121 ctcggtcggg gtgcgacgcc acctcggcct gctggaacgc gacggcctgg tcaccgccga
    21181 gacctccaag ccgaagcggg gccggcccgc tgcggtctac cggctcaccg acgcgggcct
    21241 ggagaagttc ccgcgccact acgacgaggt ggcgcgggag gcgctggcct tcctcaagcg
    21301 ccaggacgcc gccgtgctgt cccagttcct cgcctggcgc aacgagcgcc tggccgccag
    21361 ctacgcccgc cgggtggacg gtgccaccct cgccgaacgc accgaagccc tcgccgcggt
    21421 cctcagcgag cagggcttca tggctgaggt ggaacccgcc cccgacggcg gcgggttgcg
    21481 gctctgccag cacaactgca ccgtcgagca cctcgccacc gagctgcccg acctgtgcgc
    21541 ctccgaggcg aagctgttcg agcgcctgct cggcagcccc gtccagcggg aggcgacgat
    21601 cgtggacggt gccgtacgct gcgtcacccg catccaccca gaagcccgaa aaccgaacca
    21661 gaccagcaag gcgagcatga cgaacaaaga gacccgcaag cgccgggtgc ccccggcccc
    21721 gaccccgatg gcagcaagga gcgaagcctg atggccaccg atgcccgaga cctctcccag
    21781 gcgccggagc gcatcaaccc ggtcccggag aacgtcgacc tcggcgcgta caagtacggc
    21841 tggcacgacc cggtctcctc ggtcttcacc ccgaagaggg gcctctccca cgaggtcgtc
    21901 gaagagatct cccagatgaa gaacgaaccc gagtggatgc ggaagttccg ccacaagtcc
    21961 ctcgactact tcctccgccg ccccatgccc cagtggggtg gagacctcaa ccagatcgac
    22021 ttcaacaaca tctactactt cctccgcgcc accgagaagc aggcagctac atgggaggac
    22081 ctccccgccg acatcaagaa cacctacgac cggctgggca tccccgaagc ggagaagcag
    22141 cgcctagtct cgggtgtcgc ggcccagtac gaaagcgaag ttgtttacca taaggtacga
    22201 gaagacctag aggcggaagg tgtcatcttt ctggacactg ataccgcatt gcgcgagcac
    22261 gaagacgtct tccgtgagca ctttgcgacg gtcattcccg ccaacgacaa caagttttcg
    22321 gcactgaact cggctgtctg gtccggtggt tcgtacatct ggatacctga gggtgtccag
    22381 gttgagattc cgctgcaggc gtacttccgc atcaacgccc agaacatggg ccagttcgag
    22441 cggacgctga tcatcgccga gcccgggtcg agcgtgcact acgtcgaggg ctgcctgccc
    22501 gccggtgagc tggtggcgat ggccgacggc actcaccggc cgatcgagac aattggtgtc
    22561 ggcgatgacg tgctgagcca tgagggctgg ccggtccgtg tgaaggctgt gcagacacgg
    22621 gactatcgcg gaccgctcta caccttcgtg ccggtctcgc cggacaacgc attccgtgtg
    22681 actgccgagc acccgctact ggttgtgccg cgtgactccg tgctggtcaa gcgtgcgccc
    22741 cgcaacggct ggaagcccga agttgagacg gccaaactgc tcgccaccac acctga
//
LOCUS       DASWDC010000304         6407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_125908, whole genome shotgun sequence.
ACCESSION   DASWDC010000304 DASWDC010000000
VERSION     DASWDC010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125908"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..1360)
                     /locus_tag="VF995_09795"
     CDS             complement(359..1360)
                     /locus_tag="VF995_09795"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX9343892.1"
                     /translation="MSQAGTTPKMVEALGRRHLMLFSGRASRDLATEVARHLGIECGS
                     VDLRDFANGETYSRFGESVRGTDAFVIQTHSAPVNERIMEQLVMIDALKRGSAKRISA
                     VVPYYGYARQDKKGRSREPITARLVADLLDVAGADRIITVDLHSGQIQGFFDGPVDHL
                     TALPVLADYLREHLPRDLVVVAPDAGRVKTAEKLAGQLGQPLAFLHKRRSREVAHQVE
                     VKEVVGDVAGRHCIMIDDIIDTAGTIVQGAEVLMANGAEAVTAAATHGIFSGPAIDRL
                     KNAPIDEVVVTNTLPLPSDKRLDKIVVLSVARILADAIRAVFEDASVSEIFNDQNQM"
     gene            complement(1405..2856)
                     /gene="glmU"
                     /locus_tag="VF995_09800"
     CDS             complement(1405..2856)
                     /gene="glmU"
                     /locus_tag="VF995_09800"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX9343893.1"
                     /translation="MTVNVVVLAAGEGKRFKSALPKPLHQVAGRPLLWHVLAAAAPLG
                     ATRTGVVIGQGADDVRAAVEGFDFTSVPGAAAAGGAEGTTGFAVQRELRGTGDALAAA
                     LPLLPEAADGEVLVLYGDTPLLTTATLRRLLDAHRAAHATATLLTCVVDDPSGYGRVL
                     RDPDGAVSGIVEQRDATPEQAAITEMNVGVYVFARSLLSRLAELRPDNDQGEYYLTDL
                     ITLALHDGMVASCQADSEEIAGVNDRAQLAAVGAVLRRRVLDRLLADGVTVVDPATTF
                     VDVDVQVGPDTILLPLTFLEAGTRIGPGCQIGPNARLVACTVGEGATITQAVAVQAHV
                     GPRAAVGPFAYLRPGAELGPGSKVGTYVEVKKSSIGAGTKVPHLTYVGDADIGSGVNV
                     GAGTVFVNYDGFDKHHTSVGDGAFIGSATMLVAPLTVGDGAATAAGSTITRDVPPDAL
                     AIERAEQRTIDGAQARRRRRKLAEREAKRQEGG"
     gene            complement(2903..2977)
                     /locus_tag="VF995_09805"
     tRNA            complement(2903..2977)
                     /locus_tag="VF995_09805"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2943..2945),aa:Gln,seq:ttg)
     gene            complement(3035..3949)
                     /locus_tag="VF995_09810"
     CDS             complement(3035..3949)
                     /locus_tag="VF995_09810"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF002870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX9343894.1"
                     /translation="MTSTLRPPADPPVVLRAHAAAKINLALLVGERRPDGFHEIVTVL
                     QSIGLWDDLEVRLAPDGLGLEVEGEGLPPSESNLVLVAARELARRSRDLPGASFRLRK
                     GIPVAAGLGGGSADGAAALLALDRLWQLNLPAVNLHAMATEVGSDVPFCVRGGSRVGT
                     GRGERLQEVPTKGLLWWLVAISSESLATAEVYRRYDELGLARPLEGRWPDALLDALAA
                     GDLERVGRALDNDLEPAAFDLLPVLRSGKERLLEAGALGAVMSGSGPTLLALARDEEH
                     AGRIARAARGAFTRVEIARGPVPGVTFG"
     gene            complement(4042..4908)
                     /gene="rsmA"
                     /locus_tag="VF995_09815"
     CDS             complement(4042..4908)
                     /gene="rsmA"
                     /locus_tag="VF995_09815"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX9343895.1"
                     /translation="MPTTPLTPPRVRELLATHGLRPVKAFGQNYLADPNTARKVVRLA
                     AIEPGEPVLEVGPGLGSLTLALREAGARVVAVEADARLLPALAEALGPDPEVQVQVGD
                     ALRVDLGAVAPEARKLVANLPYNIAATLVLRVLAEFPQYERLTVMVQREVGERLAAAP
                     GSGAYGAVSAKVAARAAARVLAPVSRRVFIPEPHVDSVLVGLERRRHPALAGLDDRVV
                     TRVIDAAFAQRRKTLRNTLRGLGLDPPGVEALGQAAGIDLGLRAERLDLAAFAALAHA
                     LTQLPTVNAGPF"
     gene            complement(4947..5762)
                     /locus_tag="VF995_09820"
     CDS             complement(4947..5762)
                     /locus_tag="VF995_09820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9343896.1"
                     /translation="MLIDTHCHLHLLEEPADGILERAREEGVGVVVDVGVDLPSSRQA
                     VGNAARFPGVVASAGVHPHDAVTLTDQVEAELRQLLAGERVVAVGETGLDYFRDHSPR
                     EVQRAAFATHIRLARELGRALVVHCRDAWEDVLAMLRGEGAGGGDPGRVVLHCFSGDE
                     GVATRALAAGYTISFAGTVTFKNAGAQRAVCAAVPLDRMVLETDSPFLTPHPYRGQPN
                     RPWRVAVTAQTVAEVHRVTPEEVAAATTATATRAFGLSTSPPSGQPGDTAGCG"
     gene            complement(5774..>6407)
                     /locus_tag="VF995_09825"
     CDS             complement(5774..>6407)
                     /locus_tag="VF995_09825"
                     /inference="COORDINATES: protein motif:HMM:NF039287.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9343897.1"
                     /translation="EPFGSDAFRYFFQREVAFGQDGNFSWEAMVGRYNADLANGLGNL
                     ASRVTAMVERYRDGVLPGQGIPGDAEVAVQEAAVRAYADAKAALFDELAFERALAAIW
                     RFVAAANGYLSDRKPWDLAKQGLTHELELDTVLYTGAEALRVAALLTAPWLTQAAPRL
                     WEALGAPGKLAEQRLPAALAWGGMPRGARVSKVALFPRLDAEGRPAGSAS"
BASE COUNT          849 a         2280 c         2403 g          875 t
ORIGIN      
        1 gtcctccgcc tcgctcgcgc tgctcacgat cccgtaggcg atggagaaca gcagcggccg
       61 gaggtcctcg tacacctccc tacgcggtgt ctcgggctcg ctcctcgcgt ccatcgttca
      121 atcctagacc gctcagccgt ccagcctgcg acaccgcccg gatcccgagc acctctgtca
      181 cagggcgccc gtcgtgcttg tctgcccgag ctgaccgtga cttcgcgtcg atgaacggcg
      241 actcccgcga ctgcctaacg ggtctcggga gtcgggtagt tccgtagcaa cgatcgggac
      301 cccggccggg ccaccccgcc acgccaaggg gcgcagtccc gcccccgggc ccctgccctt
      361 acatctggtt ttggtcgttg aagatctcgg agacgctggc gtcctcgaag accgcgcgga
      421 tggcgtcggc gaggatgcgg gcgacggaga ggaccacgat cttgtcgagg cgcttgtcgc
      481 tggggagggg gagggtgttg gtgacgacga cctcgtcgat gggggcgttc ttgaggcggt
      541 cgatggcggg gccggagaag atgccgtggg tggcggcggc ggtgaccgct tcggcgccgt
      601 tggccatcaa cacctcggcg ccctggacga tggtgccggc ggtgtcgatg atgtcgtcga
      661 tcatgatgca gtggcggccg gcgacgtcgc cgacgacttc cttgacctcg acctggtggg
      721 cgacctcgcg gctgcggcgc ttgtggagga atgccagcgg ctggcccagc tggccggcga
      781 gcttctcggc ggtcttgacg cggccggcgt cgggggcgac caccaccagg tcacggggga
      841 ggtgctcgcg caggtagtcg gccagcaccg gcagggcggt gaggtggtcg acggggccgt
      901 cgaagaagcc ctggatctgg cccgagtgca ggtccacggt gatgatccgg tcggcgccgg
      961 ccacgtcgag caggtcggcg accagccggg cggtgatcgg ctcgcgggag cggcccttct
     1021 tgtcctggcg cgcgtagccg tagtagggga cgaccgcgct gatgcgcttg gcggaaccgc
     1081 gcttgagcgc gtcgatcatc accagctgtt ccatgatccg ctcgttgacc ggcgcggaat
     1141 gggtctggat cacgaaggcg tcggtgccgc ggacgctctc gccgaagcgg gagtaggtct
     1201 cgccgttggc gaagtcccgc aggtccaccg agccacattc gatcccgaga tggcgcgcca
     1261 cctcggtggc caggtcgcgg ctggcccgtc ccgagaacaa catcaggtgc cggcgtccga
     1321 gcgcctccac catcttgggc gtggttccag cttgactcat cggggcaccc ccctggggtt
     1381 ccccgaaccc ctcctggtca ctgcttaccc gccttcctgc cgcttcgcct cccgctcagc
     1441 gagcttgcgg cgccggcggc gcgcctgggc gccgtcgatg gtccgctgct cggcgcgctc
     1501 gatggcgagc gcgtccgggg ggacgtcacg ggtgatggtg gagccagccg cggtcgccgc
     1561 cccgtcaccg accgtcaacg gcgcgaccag catggtagcc gatccgatga aggcaccgtc
     1621 accgacactg gtgtggtgct tgtcgaagcc gtcgtagttg acgaacacgg tcccggcgcc
     1681 gacgttcacg ccgctgccga tgtcggcgtc gccgacatag gtgaggtgcg gcaccttggt
     1741 gcccgcgccg atgctggact tcttgacctc gacgtaggtg ccgaccttgc tgcccgggcc
     1801 cagttccgcg ccgggccgga ggtaggcgaa cgggccgacg gcggcgcgcg ggccgacatg
     1861 ggcctggacc gcgaccgcct gggtgatggt ggcgccctcc cccaccgtgc aggcaaccag
     1921 gcgggcgttg gggccgatct ggcagccggg gccgatgcgg gtccccgcct ccaggaaggt
     1981 gagcggcagc aggatggtgt ccgggccgac ctggacgtcc acgtccacga aggtggtggc
     2041 cgggtcgacc acggtgacgc cgtcggccag cagccggtcg agcacgcggc gccgcagcac
     2101 cgcaccgacg gcggcgagct gggcgcggtc gttgaccccg gcgatctcct cggagtccgc
     2161 ttggcagctg gcaaccatgc cgtcgtggag ggcgagcgtg atcaggtccg tcaggtagta
     2221 ctcgccctgg tcgttgtcgg gccgcagctc ggcgaggcgg gagagcaggg agcgggcgaa
     2281 gacgtagacg ccgacgttca tctcggtgat ggcggcctgc tcgggggtgg cgtcgcgctg
     2341 ctcgacgatg ccgctgaccg cgccgtcggg gtcgcgcagc acccggccgt agccggaggg
     2401 gtcgtcgacc acgcaggtca gcagggtggc cgtcgcgtgc gcggcgcggt gcgcgtcgag
     2461 cagccgccgc agcgtggcgg tggtgagcag cggggtgtcg ccgtagagca ccagcacctc
     2521 gccgtcggcg gcctccggca gcagcgggag cgcggcggcg agggcgtcgc cggtgccgcg
     2581 cagctcacgc tgcaccgcga acccggtcgt gccctcggcg ccgccggccg ccgcggcgcc
     2641 ggggaccgag gtgaagtcga agccctcgac cgcggcgcgc acgtcgtcgg cgccctggcc
     2701 gatcaccacc ccggtccgtg tcgcgccgag cggggccgcc gccgccagca cgtgccacag
     2761 cagcgggcgg cccgccacct gatggagcgg cttgggcagc gccgacttga agcgcttgcc
     2821 ctcccccgcc gccagcacca ccacattcac tgtcatgcca gtcccccgga gcgttcactc
     2881 accggcaata tcggtatcgt cgtggctggg gggcagggat tcgaaccccg acctgacggc
     2941 tccaaaggcc gccgtgctgc cattacacca ccccccagtg ggtgccccaa gggcgcagca
     3001 ggcatggtac cggtcatgcg gacatcatcc ggcatcatcc gaaggtcacc cccggaaccg
     3061 gcccgcgggc gatctcgacg cgggtgaagg cacctcgggc agcgcgggcg atgcggccgg
     3121 cgtgctcctc gtcgcgggcg agggcgagca gggtcgggcc gctgccgctc atcaccgcgc
     3181 cgagcgcgcc agcctccagc agccgctcct tgccggaccg caggacgggg agcaggtcga
     3241 aggcggccgg ttccaggtcg ttgtccaggg cgcggccgac ccgctcaagg tcgccggccg
     3301 cgagcgcgtc gagcagggcg tccggccagc ggccctccag cggccgggcc aggcccagct
     3361 cgtcgtagcg ccggtagacc tcggcggtgg cgaggctctc gctgctgatc gcgaccagcc
     3421 accagaggag gcccttggtg gggacctcct ggaggcgttc gccgcggccg gtgccgacgc
     3481 ggctgccgcc gcggacgcag aacggcacgt cgctgccgac ctccgtggcc atcgcgtgga
     3541 ggttcaccgc cggcaggttg agctgccaga gccggtcgag ggcgagcagc gcggcggcgc
     3601 cgtcggcgct gccgccgccg agcccggccg cgaccgggat gcccttgcgc agccggaagc
     3661 tggcacccgg caggtcccgc gagcgccgcg ccagctccct ggccgcgacc agcacgaggt
     3721 tgctctcgct gggcggcagg ccctcgccct cgacctccag cccgaggccg tcgggggcga
     3781 ggcggacctc caggtcgtcc cagaggccga tcgactgcag cacggtgacg atctcgtgga
     3841 agccgtcggg acgccgctcc ccgaccagca gcgcgaggtt gatcttggcg gccgcgtgcg
     3901 cacggagcac caccggcggg tcggcgggcg ggcgtagggt gctcgtcact tgcacttgct
     3961 cccctcgttt cggcggttca gcggttcggc gttcgtgtgc cgggtccatg gagacatagt
     4021 agctggaagc ttacgatcgg tttaaaacgg accagcgttg acggtcggca gctgggtcag
     4081 ggcatgagcg agcgcggcga acgcagccag gtcgagccgc tccgcccgca ggccgaggtc
     4141 gatccccgcc gcctggccga gcgcctcaac cccggggggg tccaggccga gcccccgcaa
     4201 cgtgttccgc agggtttttc gccgctgcgc gaaggcggcg tcgatcaccc gggtgaccac
     4261 ccggtcgtcg agaccggcga gcgcgggatg gcggcggcgc tccagcccga ccaggaccga
     4321 gtccacgtgt ggctcgggga tgaacacccg ccggctgacc ggggcgagca cccgcgcggc
     4381 cgcccgcgcc gccaccttgg cgctgaccgc gccgtaggcg ccggacccgg gcgcggcggc
     4441 gagccgctcg ccaacctccc gctggaccat cacggtgaga cgctcgtact gggggaactc
     4501 ggccagtacc cgcagaacca gcgtggccgc gatgttgtag ggcaggttgg cgaccagctt
     4561 gcgcgcctcg ggcgcgaccg cgcccagatc gacccgcagg gcgtctccca cctggacctg
     4621 aacctcagga tcagggccga gcgcctcggc gagcgcgggc agcagccgcg cgtcggcctc
     4681 caccgcgacc acccgggcgc cagcctcacg cagcgccagc gtgaggctgc cgagcccggg
     4741 gccgacctcc agcaccggct cgcccggctc gatcgcggcc aggcgcacga ccttgcgggc
     4801 ggtgttcggg tcggccaggt agttctggcc gaacgccttc accggccgca ggccgtgggt
     4861 ggcgagcagc tcgcgcaccc ggggtggagt cagcggtgtc gtcggcatag gtgcacggta
     4921 tccggtcagg ccgaggggcg ctgcggttat ccacagcctg cggtgtcgcc tggttgaccg
     4981 ctcggcgggc tggtggagag gccgaacgcg cgggtcgcgg tggccgtggt cgcggcggcg
     5041 acctcctcgg gagtgacgcg gtggacctcg gcgacggtct gggcggtgac ggccacccgc
     5101 caggggcggt tcggctgccc gcgatacgga tgcggggtca ggaacgggct gtcggtctcc
     5161 agcaccatcc ggtccagcgg cacggccgcg cagacggccc gctgcgcgcc cgcgttcttg
     5221 aaggtcaccg tgcccgcgaa cgagatcgtg tagccagccg cgagcgcccg cgtggcgacc
     5281 ccctcgtcgc cgctgaagca gtggagcacc acccgcccgg gatcgccgcc gcccgcgccc
     5341 tctccccgga gcatggcgag cacgtcctcc caggcgtcac ggcagtgcac caccagcgcc
     5401 ctgccgagct cccgcgccag gcggatgtgg gtggcgaagg cggcccgctg cacctcgcgt
     5461 ggcgagtggt cgcggaaata gtcgaggccg gtctccccca cggcgaccac ccgctcgccg
     5521 gcgagcagct gccgcagctc ggcctccacc tgatcagtca gggtgaccgc gtcatgcggg
     5581 tgcaccccgg cgctcgccac caccccgggg aaccgggcag cattgcccac cgcctgccgg
     5641 ctggagggga gatccacccc cacgtccacg accacgccca ccccctcctc cctggcccgc
     5701 tccaggatcc cgtcggcggg ctcctccaag aggtgcaggt ggcagtgggt gtcgatcagc
     5761 atccgcgggc aggtcagctc gccgagcccg cggggcggcc ctcggcgtcc aggcgcggga
     5821 acagggcgac cttgctgacg cgggcgcctc tcggcatgcc gccccaggcg agcgcggcgg
     5881 ggaggcgctg ctcggccagc ttgccggggg cgccgagcgc ctcccagagc cggggggccg
     5941 cctgggtgag ccagggggcg gtcagcaggg cggcgacgcg gagcgcctcg gcgccggtgt
     6001 agaggacggt gtccagctcc agctcgtggg tgaggccctg cttggcgagg tcccacggct
     6061 tgcggtcgga gaggtagccg ttggcggcgg cgacgaagcg ccagatggcg gccagggccc
     6121 tctcgaaggc cagctcgtcg aacagggcgg ccttggcgtc ggcgtaggcg cgcaccgccg
     6181 cctcctggac cgcgacctcg gcgtcgccgg ggatgccctg gccggggagg acgccgtcgc
     6241 gatagcgctc caccatcgcg gtgacccggc tggcgaggtt gccgaggccg ttggcgaggt
     6301 cggcgttgta gcggccgacc atcgcctccc aggagaagtt gccgtcctgg ccgaaggcga
     6361 cctcgcgctg gaagaagtag cggaaggcgt cggagccgaa cggctcc
//
LOCUS       DASWDC010000305        10309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1129309, whole genome shotgun sequence.
ACCESSION   DASWDC010000305 DASWDC010000000
VERSION     DASWDC010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129309"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VF995_09830"
     CDS             <1..203
                     /locus_tag="VF995_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX9343898.1"
                     /translation="ALFSFMTIAFAVFVFVVKPVNSLTMRFRRRQNPDPTLRKCPQCV
                     NDIPLAAKRCGFCTSEVAPATT"
     gene            265..498
                     /locus_tag="VF995_09835"
     CDS             265..498
                     /locus_tag="VF995_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001000 - bacterial-type RNA polymerase
                     core enzyme binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HEX9343899.1"
                     /translation="MNDSRDRLSAEFERLEPPSNSYAPRQVVTYLCTREHLVAVPFAL
                     EAEVPEVWDCRCGQPATRVEVGEGEATSGAATG"
     gene            complement(476..769)
                     /locus_tag="VF995_09840"
     CDS             complement(476..769)
                     /locus_tag="VF995_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343900.1"
                     /translation="MAPTRSHRRAVRPLYPGVVQVFALGMLGFMASLLGFRLPWHAAW
                     PLRLGLEAVVVLLLWKAVSLFRASVKAQLRLFNAQSALAYAVALQAFILLLRH"
     gene            complement(781..3396)
                     /gene="clpB"
                     /locus_tag="VF995_09845"
     CDS             complement(781..3396)
                     /gene="clpB"
                     /locus_tag="VF995_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX9343901.1"
                     /translation="MDVNAFTHKAQEALANAQRNALASDHQRIEPEHLLVALLSDPEG
                     IVFPVVRRAGADPGQLRARASEALGRIPKVYGPEQEVVLSPQTARLIERARKEAAALG
                     DQYVSTEHLLLAMTEGSDNAAKLLTGAGVTRAAILQALEQVRGSKRVTDQDPEARYQA
                     LERFGRDLTEAARAGKLDPVIGRDDEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LANRVVAGDVPDGLKDKRIVALDLAGMIAGSKFRGEFEERLKAVLDEIRESNGQIITF
                     IDELHTVVGAGAAEGAMDAGNMLKPMLARGELHMIGATTLDEYRKHIEKDAALERRFQ
                     PVLVGEPSVEDTIGILRGLKERYEVHHGVRIQDAALVAVAVLSDRYVTGRFLPDKAID
                     LVDEAASRLRIEIDSMPIEIDVLDRRAKQLEIERAALTKETDEVSRERLTKLEEELAG
                     LREQLNSMKAHWEAEKEAIQAIRATKARIEETRGEAERAERDGELSRAAELRYGVLPG
                     LERELEAANQRLAALQTERQMLKEEVDEEDVAEVVSAWTGIPVTRLMQGEMAKLLRLE
                     DELHARVVGQDEAVTAVADAIRRSRAGLSEPGRPIGNFLFLGPTGVGKTELARALAAY
                     LFDDERAMVRIDMSEYGEKHAVARLIGAPPGYVGYEEGGQLTEAVRRRPYSVILLDEI
                     EKAHPDVFNVLLQVMDDGRLTDGQGRTVDFANAVLIMTSNLGSQYLTEVFESEETIRE
                     RVMAVVRASFKPEFLNRLDEVLVFHRLSQADLTRIVDIQMDRLRRRLAARRISVALTP
                     EARRWLADRGYDPAYGARPLRRLIDKRIGDEVARRILSGEVGDGDRLLVHATKDGITF
                     AKQAPLALGGTAQGV"
     gene            complement(3455..3922)
                     /locus_tag="VF995_09850"
     CDS             complement(3455..3922)
                     /locus_tag="VF995_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9343902.1"
                     /translation="MADQDQNQRNTGPGAGSAGAGKTRPDDRAVFVISVAAELAGVHP
                     QTLRIYERKGLVRPKRTAANTRRYSERDIERLRSIQELTTLGVNLAGVARVIALEEEV
                     DRLEARIAALEQALATQARRHREELEAARRAARRDLVRYSSSIVRYKPPRRYD"
     gene            complement(3933..5057)
                     /gene="dnaJ"
                     /locus_tag="VF995_09855"
     CDS             complement(3933..5057)
                     /gene="dnaJ"
                     /locus_tag="VF995_09855"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX9343903.1"
                     /translation="MNNPEYLEKDFYATLGVAQDASAQDVKKAYRKLAQKLHPDANPG
                     DRTAEERFKEVGRAYAVLSDPKKRAEYDEARRLVSAGGFGGFPGGYGGGQRVRVEDLG
                     DFGGLGGLFGNIFGGGGRAQQTARRGRDVESEVTLGFEEAVRGATIPLQLRGPARCVT
                     CQGTGARPGTLPRPCPTCGGRGTITRDQGLFGLSSPCPTCQGRGTVIDDPCTTCRGSG
                     TQIRTRELKVRIPIGVNDGAVIRLKGQGEPGMNGAPGGDLIVKVHVTPHPIFARRAQD
                     LVMTVPVTFAEAALGTELTVPTLDGSVTLKVPPGTQNGRTFRVKGRGVPSSARKLTGD
                     LLVTVQVSVPEKLSRRERELLREFASLSDTSPRSHLNMER"
     gene            complement(5060..6115)
                     /gene="grpE"
                     /locus_tag="VF995_09860"
     CDS             complement(5060..6115)
                     /gene="grpE"
                     /locus_tag="VF995_09860"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX9343904.1"
                     /translation="MGEQVDVTNDAQRAPEPDEPATTQPGGEAEPAAQQPGAAAPETA
                     PEAGEEPAGGQPATDAVAAAAEVGAEAAGKAPAPEPEPRAKGHGEAEEEGEGGGPAAG
                     DSAQDAAAAQAGKDTATDDGAGEPEPAAAATATAVEGEAPAEPQREGQPEAAEPQIAA
                     AAAGSAPEAATKAEAEAGTEAGTEAPAGQRAPDEGDSEGEAMMADLVAARAEAESYLD
                     DLRRLQAEFDNYRKRMLREQTNRIASASQALVAKLLPVLDNFELAVSAAEQSRDFDRM
                     VKGVEMVFGQLKEVLDNEGLVLIEAEGKPFDPERHEAVVAVEQEGAETGNVVDVIRKG
                     YEFNGRVLRPAMVKVAK"
     gene            complement(6252..6743)
                     /locus_tag="VF995_09865"
     CDS             complement(6252..6743)
                     /locus_tag="VF995_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9343905.1"
                     /translation="MSVMRWDPFRDLLSIQEEMNQLFNRAFGRSGGEAAGESGRTGRM
                     WAPAIDISERKDAYVVTVEVPGVKQDDIDITLEDGLLTISGERKLTQESSDQQYHRVE
                     RRYGAFRRSITLPSQVRADAIEASFDNGVLEVVLPKAEEAKPKKITVRSGGTRQAISD
                     AKS"
     gene            complement(6788..8674)
                     /gene="dnaK"
                     /locus_tag="VF995_09870"
     CDS             complement(6788..8674)
                     /gene="dnaK"
                     /locus_tag="VF995_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017603069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX9343906.1"
                     /translation="MVKAVGIDLGTTNSVVAVLEGGEPTVIPNAEGARTTPSVVALSK
                     TGEVLVGEVAKRQAVTNPDRTIRSVKRHMGEGSWRFRADGKEFSPQQISAFILQKLKR
                     DAEAYLGDSVSQAVITVPAYFDDAQRQATKEAGQIAGLEVLRIINEPTAASLAYSLDK
                     ENDQTILVFDLGGGTFDVSLLDIGEGVIEVQATSGDMHLGGDDWDQRIVDWLLKTFKN
                     NHGIDLSGDKMAMQRLKEAAEKAKIELSSTLETTINLPFITATPEGPLHLEQRLTRGE
                     FERMTQDLVERCKGPFDRAITDWKAKTGKDLGQINHVVLVGGSTRMPMVFEFVKKLTG
                     GKEPHKGVNPDEVVAVGATLQAGVLKGEVKDILLLDVTPLSLGIETKGGVMTKLIERN
                     TTIPTRRSEVFTTADDGQSEVDVKVFQGEREMTYGNKLLGNFQLVGIPPAPRGVPQIE
                     VTFDIDANGIVNVSAKDRGTGKEQSMTITGGTALPKGDIDRMVREAEQYAEEDRRRRE
                     AAEAKNRAEQLVYQTEKSLKDYGDKISDSDRASVESALATLKEKLAGDDTVAITSATE
                     ALQQASYKLAEAVYAQAQPGPGATQSGATTTGGEPGSGAANEGDVVDAEIIDEGEGEE
                     QKGA"
     gene            8993..10117
                     /locus_tag="VF995_09875"
     CDS             8993..10117
                     /locus_tag="VF995_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX9343907.1"
                     /translation="MSAPKLRHHELRPVPASVSAPEPDLPLDVQLAGTPYLDLVFSGL
                     TATPRLGTEIRTEGLGSSPGGVANLAVALRRLDLTVGLAATFADDVYGTYLWHTLGEQ
                     EGVDLSASRRVGGWVTPLTVSLAYQRERSMITYEQPPPLAAAELLAQAPRARCFLTHI
                     DHVQASWLPALRAAGARIFADVGWDSSERWSAAVLDNLAMVDVFMPNAVEAMAYTRTS
                     SPQAALDVLAGLVPVAVVKCGPDGAIAVSRETGERVSEPAIGVEALDPTGAGDVFGAA
                     IVYGSLQQWPLAERIRFANLCAGLSVRFYGGSLSAPCWHEVAGWCRELDADPDALDKH
                     KRAYHYLREHYARTSRRVQCRRAQPTIDTACLAPPLASNG"
     gene            10125..>10309
                     /locus_tag="VF995_09880"
     CDS             10125..>10309
                     /locus_tag="VF995_09880"
                     /inference="COORDINATES: protein motif:HMM:NF014148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 4"
                     /protein_id="HEX9343908.1"
                     /translation="MPASSRRITVVGAGSAYLPGVIRGLLHRADALAGAELVLHDPDT
                     GHLRLMEALAGKMIAAAG"
BASE COUNT         1313 a         3653 c         3606 g         1737 t
ORIGIN      
        1 acgcgctgtt cagcttcatg accatcgcct ttgccgtgtt cgtcttcgtg gtcaagccgg
       61 tcaacagcct cacgatgcgg ttccggcgcc gccagaaccc cgacccgacg ctgcgcaagt
      121 gcccgcagtg cgtcaacgac atcccgctcg ccgccaagcg ctgcggtttc tgcaccagcg
      181 aagtggcccc cgcgacgacg tagggtcgtg ggtgctagcc tgaatcacgg cgtccccagc
      241 acgccagaaa ggcaaggagc agcgatgaac gacagccggg accgcctctc ggcggagttc
      301 gaacggctcg aaccgccctc gaactcctac gccccccggc aggtggtcac ctacctctgc
      361 acccgcgagc atctcgtggc cgtgccgttc gcgctcgagg ccgaagtccc cgaggtgtgg
      421 gactgccgct gcggccaacc cgccacccgg gtcgaggtcg gcgagggcga agccactagt
      481 ggcgcagcaa caggatgaac gcctgcagcg ccaccgcgta ggccagcgcc gactgggcgt
      541 tgaagaggcg cagctgcgcc ttgaccgagg ctcggaacag gctcacggcc ttccagagga
      601 gcaagacgac caccgcctcc aggccaaggc gcagcggcca ggcggcgtgc cagggcagcc
      661 tgaagccgag cagtgaggcc atgaacccga gcatcccgag ggcgaacacc tgcaccacgc
      721 ccgggtagag cggcctgacc gcccggcgat gactgcgcgt cggtgccata cagccagagt
      781 ctagacgccc tgggcggtgc cgcccagcgc cagcggggcc tgcttggcga acgtgatgcc
      841 gtccttggtg gcgtggacca gcaggcggtc gccgtcgccg acctcgcccg agaggatccg
      901 gcgggcgacc tcgtcgccga tccgcttgtc gatcaggcgg cgcagcggcc gggcgccgta
      961 ggcagggtcg tagccgcggt cggcgagcca gcgcctggcc tccggggtca gggcgacact
     1021 gatgcggcgg gcggccaggc ggcgccgcag gcggtccatc tggatgtcca caatcctggt
     1081 gaggtcggcc tggctgaggc ggtggaagac cagcacctcg tcgagccggt tgaggaactc
     1141 cggcttgaac gacgcccgca ccacggccat gacccgctcg cggatggtct cctccgactc
     1201 gaagacctcg gtgaggtact gggagccgag gttggaggtc atgatcagca ccgcgttggc
     1261 gaagtcgacc gtgcgcccct gcccgtcggt gaggcgcccg tcatccatta cctgcagcag
     1321 cacgttgaag acgtccgggt gggccttctc gatctcgtcg agcaggatca cgctgtaggg
     1381 gcggcggcgc accgcctcgg tgagctggcc gccctcctcg tagccgacgt agccgggcgg
     1441 cgcgccgatc aggcgggcga cggcgtgctt ctcgccgtat tcgctcatgt cgatgcgcac
     1501 catcgcgcgc tcgtcgtcga acaggtaggc ggcgagggcc ctggccagct cggtcttgcc
     1561 gacgccggtg gggccgagga acaggaagtt gccgatcggg cggcctggct cggacagccc
     1621 ggcgcgggag cggcggatcg cgtcggcgac ggcggtgacg gcctcgtcct ggccgaccac
     1681 gcgggcgtgc agctcatcct cgaggcgcag cagcttggcc atctcgccct gcatcaggcg
     1741 ggtcaccggg atgccggtcc aggcggagac gacctcggcg acgtcctcct cgtcgacttc
     1801 ctccttgagc atctgacgct cggtctgcag cgcggcgaga cgctggttgg ccgcctccag
     1861 ctcgcgctcc aggccgggca ggacgccgta gcgcagctcg gcggcgcggg acagctcgcc
     1921 gtcgcgctcg gcgcgctcgg cctcgccgcg ggtctcctcg atgcgggcct tggtggcgcg
     1981 gatcgcctgg atggcctcct tctcggcctc ccagtgagcc ttcatcgagt tgagctgctc
     2041 gcgcaggccg gccagctcct cctccaattt cgtcaggcgc tcgcgcgaga cctcgtcggt
     2101 ctccttggtg agggcggcgc gctcgatctc gagctgcttg gcgcggcggt caagcacgtc
     2161 gatctcgatc ggcatcgagt cgatctcgat ccggagccgg gaggcggcct cgtcgaccag
     2221 gtcgatcgcc ttgtcgggca ggaagcggcc ggtcacgtag cggtcggaga gcactgccac
     2281 cgcgaccagc gcggcatcct ggatgcgcac gccgtggtgg acctcgtagc gctccttgag
     2341 gccgcggagg atgccgatgg tgtcctcgac gctcggctcg ccgaccagca ccggctggaa
     2401 gcggcgctcc agggcggcgt ccttctcgat gtgcttgcgg tactcgtcca gggtggtggc
     2461 gccgatcatg tgcagctcgc cgcgggccag catcggcttg agcatgttgc cggcgtccat
     2521 ggccccctcg gcggcaccgg cgccgaccac ggtgtgcagc tcgtcgatga aggtgatgat
     2581 ctggccgttc gactcgcgga tctcgtcgag gaccgccttg aggcgctcct cgaactcgcc
     2641 gcggaacttc gagccggcga tcatgccagc gaggtcgaga gcgacgatgc gcttgtcctt
     2701 gaggccgtcg ggcacgtctc cggcgaccac ccggttggcc aggccctcga cgatggcggt
     2761 cttgccgacg ccgggctcgc cgatcagcac tgggttgttc ttggtgcggc gcgatagcac
     2821 ctgcacgacg cggcggatct cgtcgtcgcg gccgatcacc gggtcgagct tgccggcgcg
     2881 ggcggcctcg gtgaggtcgc ggccgaaacg ctccagcgcc tggtacctgg cctcggggtc
     2941 ctggtcggtg acgcgcttgc tgccccggac ctgttcgagc gcctgcagga tcgccgcgcg
     3001 ggtcacgccc gcgccggtga gcagcttggc ggcgttgtcg gagccctcgg tcatggccag
     3061 cagcaggtgc tcggtggaga cgtactggtc gccgagagcg gcggcctcct tgcgggcacg
     3121 ctcgatcagc cgggcggtct ggggcgacag cacgacctcc tgctcgggcc cgtagacctt
     3181 ggggatgcgg ccgagcgcct cgctggcgcg ggccctgagc tggcccgggt cggcgccggc
     3241 gcggcgcacc accgggaaga cgatcccctc gggatcggag agcagggcga ccagcaggtg
     3301 ctccggctcg atccgctggt ggtctgaggc gagcgcgttg cgctgggcgt tggccagcgc
     3361 ctcctgtgcc ttgtgggtga acgcgttgac atccatggat acttcaactt cttcgtggta
     3421 ttcgacggct tctcgagttc ttctcttctc cggctcagtc gtagcggcgt ggcggcttgt
     3481 agcgaacgat cgaggaggag tagcggacca ggtcacggcg ggccgcgcgg cgggccgcct
     3541 ccagctcctc gcggtgtcgg cgggcctgcg tcgccagcgc ctgctccaag gcggcgatgc
     3601 gcgcctccag ccggtcaacc tcctcctcca gtgcgatcac ccgggcgacc ccggcgaggt
     3661 tgacgcccag ggtggtcagc tcctggatcg agcgcaggcg ctcgatgtca cgctcggagt
     3721 agcggcgcgt gttggccgcg gtccgcttgg gccgtaccag acccttgcgc tcgtagatgc
     3781 gcagggtctg gggatgaacg ccggccagct cggccgccac ggagatgacg aatactgccc
     3841 ggtcgtcggg ccgggtcttc ccggctccgg ccgatccggc gccgggtccg gtgttcctct
     3901 ggttctgatc ctgatccgcc atggagatcg cctcaccgtt ccatgttcag gtgcgaccgt
     3961 ggtgaggtgt ccgagaggga ggcgaactcg cgcagcagct cccgttcgcg gcgagaaagc
     4021 ttctcgggca cgctcacctg cacagtgacc agcaggtccc cggtgagctt gcgggccgag
     4081 gacggcaccc cgcggccctt cacccggaag gtgcggccgt tctgggtgcc gggcgggacc
     4141 ttcagggtga ccgagccgtc cagggtgggc acggtcagct cggtgccgag cgccgcctcg
     4201 gcgaaggtga ccggcacggt catgaccagg tcctgggcgc ggcgcgcgaa gatcgggtgc
     4261 ggggtgacgt ggaccttgac gatcaggtcg ccgccggggg cgccgttcat gcccggctcg
     4321 ccctggccct tcaggcggat cacggcgccg tcgttgacgc cgatcgggat gcgcaccttc
     4381 agctcgcggg tgcggatctg cgtgccgctg ccgcggcagg tggtgcaggg gtcgtcgatg
     4441 accgtgccgc gaccctggca ggtggggcat gggctggaga ggccgaacag cccctggtcg
     4501 cgggtgatcg tgccgcggcc gccacaggtc gggcaggggc gcggcagggt ccccggacgg
     4561 gcgccggtgc cctggcaggt gacgcagcgg gcggggccgc gcagctgcag ggggatggtg
     4621 gcaccgcgga ccgcctcctc gaagccgagg gtgacctcac tctcgacgtc gcgcccgcgg
     4681 cgggcggtct gctgggcacg gccgccgccg ccgaagatgt tgccgaacag gccgccaagc
     4741 ccgccgaagt cgcccaggtc ctcgacgcgg acgcgctggc cgccgccgta gcctccggga
     4801 aagccgccga atccgcccgc gctgaccagc cggcgcgcct cgtcgtactc ggcgcgcttc
     4861 ttgggatcgg acaggacggc ataggcgcgc ccgacctcct tgaaccgctc ctcggcggtg
     4921 cggtcgccag gattggcgtc cgggtgaagc ttctgagcga gcttgcggta ggccttcttg
     4981 acgtcctggg cgctggcgtc ctgcgcaaca cccagggtcg cgtagaagtc cttttcgagg
     5041 tactccgggt tgttcacgcc tacttagcta ccttcaccat ggcgggtcgc aggacccggc
     5101 cgttgaactc ataacccttc cggatcacgt cgacgacgtt gccggtctcg gcaccctcct
     5161 gctcgacggc gacgactgcc tcgtgccgct cgggatcgaa gggcttgcct tctgcctcga
     5221 tcaagaccag gccctcgttg tcgagcacct ccttgagctg gccgaagacc atctcaacgc
     5281 ccttgaccat gcggtcgaag tcgcgcgact gctcggcggc ggacaccgcc agctcgaagt
     5341 tgtcgagcac cggcaggagc ttcgccacca gcgcctgcga cgcggaagcg atccggttgg
     5401 tctgctcccg cagcatgcgc ttgcggtagt tgtcgaactc cgcctggagc cggcgcaggt
     5461 cgtcgaggta gctctcggcc tcggcccgcg cggccaccag atcggccatc atcgcctcgc
     5521 cttcactgtc gccttcatcg ggggcgcgct gacccgccgg cgcctcagtt ccggcctcag
     5581 ttccggcctc ggcctcggct ttggtcgcgg cttcgggcgc cgacccggct gcggccgctg
     5641 cgatctgcgg ctcggccgcc tcgggctgcc cctcgcgctg cggctcggca ggcgcctcgc
     5701 cctccaccgc ggtggccgtg gcggcggcag cgggctcggg ctcgcccgcc ccgtcgtcgg
     5761 tggcggtgtc cttcccggcc tgggcggccg cggcgtcctg ggcgctgtcg ccggcagcgg
     5821 gaccgccgcc ctcgccctcc tcctcggcct cgccgtgacc tttggcgcgc ggctcaggct
     5881 ccggagctgg cgccttgccg gccgcctcgg caccgacctc ggcggcggcc gccacggcgt
     5941 cggtggcggg ttggccgccg gccggctcct cgccggcctc tggcgccgtc tcgggagcag
     6001 ccgcgccggg ctgctgcgcc gccggctctg cttcgccgcc gggctgggtg gtggccggtt
     6061 cgtccggctc aggagcgcgc tgtgcgtcgt tggtcacgtc cacttgctcg cccatccgtc
     6121 gtcgtcggtt cttgctcttc accgttcccc tttcaaaggc gccgcccagg gagtgggccg
     6181 gggccgcgcc tttgccggga ggagggaggg ggaccacgcc tccctcctcc ggtcacacgt
     6241 cgtcgcggtt ctcagctctt ggcgtcgctg atcgcctggc gggtgccgcc gctgcgcacc
     6301 gtgatcttct tgggcttggc ctcctccgcc ttgggcagga cgacctcgag cacgccgttg
     6361 tcgaatgagg cctcgatcgc gtcggcccgc acctggctcg gcagcgtgat cgagcggcgg
     6421 aaggcgccgt atcgccgctc cacccggtgg tactgctggt ccgaggactc ctgggtcagc
     6481 ttgcgctccc cggagatggt gagcagccca tcctcgagcg tgatgtcgat gtcgtcctgc
     6541 ttgaccccgg gtacctcgac agtgaccacg taggcatcct tgcgctcgga gatgtcgatc
     6601 gccggcgccc acatgcggcc ggtgcgaccg ctctcccccg ccgcctcacc gccggaacgg
     6661 ccaaaggcgc ggttgaagag ctggttcatc tcctcctgaa tggagagcag gtcccggaac
     6721 gggtcccacc gcatcacgga cattgtgtgt ggttccctcc ttcgtcttgt cccgaggggg
     6781 ctcggtgtca ggcccccttc tgctcctcac cttcaccttc gtcgatgatc tcggcgtcga
     6841 ccacgtcgcc ctcgttggca gcgccgctgc caggctcgcc gccggtggtg gtggcgccgc
     6901 tctgggtcgc gccgggaccg ggctgggcct gggcgtagac cgcctcggcc agcttgtagc
     6961 tcgcctgctg cagcgcctcg gttgccgagg tgatcgccac ggtgtcgtcg ccggcgagtt
     7021 tctccttcag cgtggcgagc gccgactcca cactggcacg gtcggagtcg gagatcttgt
     7081 cgccgtagtc cttgaggctc ttctcggtct ggtagaccag ctgctcggcg cggttcttgg
     7141 cctcggcggc ctcacgccgg cggcggtcct cctcggcgta ctgctcggcc tcgcggacca
     7201 tgcggtcgat gtcacccttt ggcagcgcgg tgccgccggt gatggtcatc gactgctcct
     7261 tgccggtacc gcggtccttg gcggacacgt tgacgatgcc gttggcgtcg atgtcgaagg
     7321 tgacctcgat ctgcggcacg ccacgcggcg cgggcgggat gccaaccagc tggaagttgc
     7381 ccagcagctt gttgccgtag gtcatctcgc gctcgccctg gaagaccttc acgtcgacct
     7441 cggactggcc gtcgtcggcg gtggtgaaga cctcgctgcg gcgcgtcggg atggtggtgt
     7501 tgcgctcgat cagcttggtc atcacgccgc ccttggtctc gatgccgagg gacagcgggg
     7561 tgacgtcgag cagcaggatg tccttgacct cgcccttgag cacgccggcc tgcagggtgg
     7621 cgcccaccgc cacgacctcg tccgggttga cgcccttgtg cggctccttg ccgccggtga
     7681 gcttcttgac gaactcgaac accatcggca tgcgcgtcga gccgccgacc agcaccacgt
     7741 ggttgatctg gccgaggtcc ttcccggtct tggccttcca gtcggtgatg gcgcggtcga
     7801 acgggccctt gcagcgctcc accaggtcct gggtcatgcg ctcgaactcg ccccgggtga
     7861 ggcgctgctc gaggtgcagc gggccctccg gcgtggcggt gatgaacggc aggttgatgg
     7921 tggtctctag ggtcgaggac agctcgatct tggccttctc ggctgcttcc ttgaggcgct
     7981 gcatcgccat cttgtcgccg gacaggtcga tgccgtggtt gttcttgaag gtcttcagca
     8041 gccagtcgac gatgcgctgg tcccagtcgt cgccgccgag gtgcatgtcg ccggaggtcg
     8101 cctggacctc gataacgccc tcgccgatgt cgagcaggga aacgtcgaag gtgccgccgc
     8161 ccaggtcgaa gaccaggatg gtctggtcat tctccttgtc gagcgagtag gccagcgacg
     8221 cggccgtcgg ctcgttgatg atgcgcagga cctcaagccc ggcgatctgg ccggcctcct
     8281 tggtggcctg gcgctgggcg tcgtcgaagt aggcgggaac ggtgatgacc gcctggctca
     8341 cgctgtcgcc caggtacgcc tctgcgtccc gcttgagctt ctgcaggatg aacgcgctga
     8401 tctgctgggg ggagaactcc ttgccgtcag cgcggaagcg ccaggagcct tcccccatgt
     8461 ggcgcttgac cgagcggatg gtcctgtcgg gattggtgac ggcctgccgc ttggcaacct
     8521 cgccaaccag gacctcgccg gtcttcgaca gggcgaccac cgagggggtg gtgcgggcac
     8581 cctcggcgtt ggggatgacc gtcggctcac cgccttcaag cacggcgaca accgagttcg
     8641 tggtccccag gtcgatccct accgccttga ccatctgatc acctcttgct gtggaattga
     8701 cttgcgacac gaccggccgg gacgacgagg gggctcgtcg cttcaccggc acggccgggt
     8761 gctcgacgtc caaggtagtg acttgagtga gtcgttgtca agtccgttgc gcgtgatgct
     8821 ttaccttgcc ttctacctgg ggttttagtg gtccccatgg ccgccggacc acccgggcgc
     8881 cggcttgtcc ttgcccctgg ttaccctttg gcccacgcta gagggaatcg atcccgtccg
     8941 ctccgactcc tgtctgggcc catttcccac cgaactggtg gaggtagatg ccttgagcgc
     9001 acccaagctg cggcaccacg aactccggcc cgtgcccgcc agcgtctccg cgccggaacc
     9061 ggacctgccg ctggatgtgc agctcgccgg tactccctac ctcgacctgg tgttctccgg
     9121 gctcaccgcc acgccgcggc tcggcaccga gatccgcacc gagggcctcg gctcctcccc
     9181 cggcggggtc gccaacctcg cggtggcgct gcgccgcctg gacctcaccg tcgggctggc
     9241 agccaccttc gccgacgacg tctacggcac ctacctgtgg cacaccctcg gcgagcagga
     9301 gggcgtcgac ctgtcggcct cgcgccgagt gggcggctgg gtcaccccgc tcaccgtgtc
     9361 gctcgcctac cagcgcgaac gcagcatgat cacctacgag cagccgcccc cgctggcagc
     9421 ggccgagctg ctggcgcagg ccccgagggc gcgctgcttc ctcacccaca tcgaccacgt
     9481 acaggccagc tggctgccag ccctgcgcgc cgccggcgca cggatcttcg ccgacgtcgg
     9541 ctgggactca agcgagcgct ggtcggcggc ggtgctcgac aacctcgcca tggtcgacgt
     9601 gttcatgccg aacgcggtcg aggcgatggc ctacacccgc acctccagcc cccaggccgc
     9661 cctcgacgtg ctcgccgggc tggtcccggt ggcggtggtc aagtgcggcc ccgatggcgc
     9721 catcgccgtc tcgcgcgaaa ccggcgagcg ggtcagcgag cccgcgatcg gcgtcgaggc
     9781 gctcgacccg accggcgccg gcgacgtgtt cggcgccgcg atcgtctacg gcagcctcca
     9841 gcagtggccc ctcgccgagc ggatccgctt cgccaacctg tgcgccggcc tctcggtgcg
     9901 cttctacggc gggtcgctgt ccgcgccctg ctggcacgag gtcgccggct ggtgccgcga
     9961 gctggacgcc gacccggacg ccctcgacaa gcacaagcgc gcctaccact acctgcgcga
    10021 gcactacgcg cgcaccagcc gccgcgttca gtgccgccgg gcgcagccca ccatcgacac
    10081 ggcctgcctc gcgccgcccc tcgcctcgaa cgggtaggcc cgacatgcca gccagctcca
    10141 ggcggatcac ggtcgtcggc gccggcagcg cctacctgcc cggcgtgatc cgcggcctgc
    10201 tgcaccgcgc cgacgccctg gccggcgccg agctggtcct gcacgacccc gacaccggcc
    10261 acctgcggct gatggaggcc ctggcgggca agatgatcgc cgcggccgg
//
LOCUS       DASWDC010000306         8384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1416826, whole genome shotgun sequence.
ACCESSION   DASWDC010000306 DASWDC010000000
VERSION     DASWDC010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416826"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /gene="ribH"
                     /locus_tag="VF995_09885"
     CDS             <1..417
                     /gene="ribH"
                     /locus_tag="VF995_09885"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX9343909.1"
                     /translation="LLAGALQELRRHGVAAGDVDVAWVPGAFELPVAVQRLAGSGRYA
                     AVVALGAVIRGATPHFDYVAGEAAAGLGAVARQSGVPVALGVLTTDTMEQALDRAGGK
                     AGNKGAEAALVALEMASLLDAIDKDNDNDQNDENEE"
     gene            435..1310
                     /gene="hisG"
                     /locus_tag="VF995_09890"
     CDS             435..1310
                     /gene="hisG"
                     /locus_tag="VF995_09890"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX9343910.1"
                     /translation="MLKLVLPKGSLERATLALFDAADLPVKRGGERDYHGTIDDHRVE
                     RVSLLRPQEIPRYVEEGFFDLGITGRDWIEETGAKVVSLTELPYSKVTANPVRVVLAV
                     HRDLGVTRPEDLPAGLRISTELPSLTRRFFEQRNLPVRIFPSYGATEAKVPEIVDAIV
                     DLTETGSTLRAHGMVVIATLLTSRTEVIANPAAAIDPERRQAMDDLVTLLVGAATARS
                     KTLIKLNVAAADLPAVLEVLPAMKAPTVTALAGSGFHAVETVVDKSGVNRLIPTLKAA
                     GAFDILELPIAKIVA"
     gene            1473..1676
                     /locus_tag="VF995_09895"
     CDS             1473..1676
                     /locus_tag="VF995_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX9343911.1"
                     /translation="MPEGTVKWFSNEKGYGFIARNGGDDVFVHFSAIQIDGYKTLSEG
                     QAVEFEITNGPKGPQASNVRPLA"
     gene            complement(1780..2175)
                     /locus_tag="VF995_09900"
     CDS             complement(1780..2175)
                     /locus_tag="VF995_09900"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEX9343912.1"
                     /translation="MTEDADQIQQMREQLTAVSAGDVVAEAAAQLVTFAYLRLGLPAE
                     QNAEYRDLDAARLLIDAFGGLLEATEGRLGAYEPELAQALASLRLNYAELLRREQQPG
                     EPPPPEGGEGEEDSRLHRPPSGLWVPGQP"
     gene            2466..3110
                     /gene="infC"
                     /locus_tag="VF995_09905"
     CDS             2466..3110
                     /gene="infC"
                     /locus_tag="VF995_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX9343913.1"
                     /translation="MSVEPRINDRIRAAQVRVVGADGEQLGVMETQVALRMASTSDLD
                     LVEVAPLAKPPVVKLMDYSKFKYEQGIKAKEARRKQSLVVLKEMKMRPKIDDHDYETK
                     KGHVERFLKQGHKVKVTIMFRGREMAHTELGRRLLERLATDIVEIAQVDSPARQDGRN
                     MIAVFSPRKNPGGGTPKGGQSPQAAPQTQGPPPGQGDHGPGSNGRAPSTSATAT"
     gene            3187..3381
                     /gene="rpmI"
                     /locus_tag="VF995_09910"
     CDS             3187..3381
                     /gene="rpmI"
                     /locus_tag="VF995_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX9343914.1"
                     /translation="MPKMKTHRGAASRFGVTKRGKLVRRHTKLNHILEKKSPGTKRRL
                     GREGEVAKGDRRSVRRMLGI"
     gene            3434..3799
                     /gene="rplT"
                     /locus_tag="VF995_09915"
     CDS             3434..3799
                     /gene="rplT"
                     /locus_tag="VF995_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX9343915.1"
                     /translation="MARVKRAVHGAKAKRTIMEQAKGYRSSRGRHLRVAKEQVMHSLR
                     YAYRDRRARKGEFRSLWIARINAAARAEGLTYNRFIAGLRGAGVEVDRKVLADIAVTD
                     PAAFAALVATAREGLNASP"
     gene            3807..5420
                     /locus_tag="VF995_09920"
     CDS             3807..5420
                     /locus_tag="VF995_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX9343916.1"
                     /translation="MPAPAATVNVITSARNPAVLAARKLARQRRARATDGRFLVEGAR
                     TIQAALDAGQVPAALLATEAAAARHGGLLARARRAGAGVTVVAPGLLDGIAQTVTPQG
                     LVAVLERVTRPLAALPPDPRLVCVLAEVRDPGNAGAVLRAADAFGADALATTAGSVDL
                     ESPKAVRAAAGSLFHLPVIADAPWPELATQLRRRGLLLIGADPHATATVDDAPLERPC
                     ALIFGNEAHGLDDELRRSLDLTVRVPHDGRAESLNLAAAAAVLLYETARRQRHARPAP
                     DPAPEPAPQAEAKAEADAAVAELITTLAHDLRSPLTGIKGFTATLLHRWERFSDEQKQ
                     HMLRTISADADRLTRLIKELLDVARIEAGKLELRREMVDLPAIAEGVAGRFEPADRGP
                     RGARLAIVFPDGFPDLYADRDKLEQVLTNLVENAVNHTEDGTVTIVGEVAGDAVEVAV
                     RDPGSGVPAEQLPQVFSKFWRRPDPLRGAPRGSGLGLYVACGLVEAHGGRIWATSPPS
                     GGLELRFRLPRGGLELAGIHEHQPAPSPS"
     gene            5536..6624
                     /gene="pheS"
                     /locus_tag="VF995_09925"
     CDS             5536..6624
                     /gene="pheS"
                     /locus_tag="VF995_09925"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX9343917.1"
                     /translation="MPNEANTPQQPSQPATDVDLDAVVKDGLAAIAAAGDLDALQQAD
                     QAHLQRRSPLGEAQRSLGRLDAAARRQLGQQVNQARAELQAALADRRAELEAARDAAL
                     LEAERIDVTLPGRIPPRGAVHPISRTAEEIIDIFIGLGYRVAEGPEVETDWYNFEALN
                     FPPDHPARSMQDSLSLEATDPNGVPLLLRTHTSPMQIRSMLAAPPPLYVVIPGRCYRA
                     DTPDATHVPVFNQIEGLAVDTGITMGDLKGTLLAFARAYFGDDRQVRLRPSYFPFVEP
                     GAEVDVSCFVCGGRGSGCRTCKGEGWIELLGAGMVHPNVLRAGGYDPDQVSGFAFGMG
                     IERPYMLRSGLADIRTLTDNDLRWLASV"
     gene            6653..>8384
                     /gene="pheT"
                     /locus_tag="VF995_09930"
     CDS             6653..>8384
                     /gene="pheT"
                     /locus_tag="VF995_09930"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9343918.1"
                     /translation="MRVPLTWLAVHVDLGDVSVQALADQLTFAGLEIETIDWVGQDVA
                     GVITARVLRIAPHPDADRLALVTIDPGPGGEPREVVCGAHNYAPGDVVPWAAPGAKLP
                     GGLEIGRRKVRGRWSDGMLASARELGVFDDHSGILILPPDTPVGVDLTEEEGVGLRDA
                     VFDIKTFPNRGDTLSIRGVAREAALVLGTELKPLDLTVPERGPQAATLATVEVDDAEG
                     CPIYYARVIEGVDAGRPAPLWMARRLYLYGQRPLGAVVDVTNYLLIDQGQPLHAFDLD
                     RIPSHRILVRRAQPGEVIRTLDGRDRHLTPEDTLISAGDEPLALAGIMGGEDSEVRPD
                     STRILLEAAHFPPAFVRRTTRRLGMSTEGAQRWARGVDPAGAGPIGDQAARLIAELAG
                     GTVATGRLQDGPGVPPREAVRLDWRRSAQRLGAPADPAFAAPYLEGVGCQVQLEPDSA
                     ALRAVPPSWRFDIEGWADLEEEVARRWGYDKLPATLPKATGGRRTAEQRLRRRTRDLL
                     AGMGLTEVQPYPFTSQGALDRLELSASDQRRRALRLANPLSEESPELRTTLLPGLAEA
                     ARRNLEKGLAG"
BASE COUNT         1282 a         3309 c         2737 g         1056 t
ORIGIN      
        1 ctgctggccg gggcactgca ggagctgcgg cgccacggcg tcgccgccgg cgacgtggac
       61 gtcgcctggg tgccgggcgc gttcgagctg cccgtggcgg tgcagcggct ggccggctcc
      121 ggccgctacg ccgccgtggt cgcactcggc gcggtgatcc gcggcgccac cccgcacttc
      181 gactacgtcg ccggggaggc cgccgccggc ctcggcgcgg tcgcccgcca gagcggcgtc
      241 ccggtcgccc tcggcgtgct caccaccgac accatggagc aggccctgga ccgcgcgggc
      301 ggcaaggcgg gcaacaaggg cgccgaggcc gccctggtcg ccctcgagat ggccagcctg
      361 ctggacgcca tcgacaagga caacgacaac gaccagaacg acgagaacga ggagtagagg
      421 agtaggggag tcctgtgctc aagctggtat tgccgaaggg aagtctggaa cgggccacgc
      481 tggccctgtt cgacgccgcc gacctgcccg tcaagcgcgg cggggagcgc gactaccacg
      541 gcaccatcga cgaccaccgg gtcgagcgcg tctctctgct gcgcccccag gagatccccc
      601 gctacgtcga agagggcttc ttcgacctcg gcatcaccgg ccgtgactgg atcgaggaga
      661 ccggcgccaa ggtcgtctcc ctcaccgagc tgccctactc caaggtgacc gccaacccgg
      721 tgcgggtcgt cctggccgtc caccgcgacc tcggcgtcac ccgccccgag gaccttcccg
      781 ccggcctgcg gatctccacc gagctcccga gcctcacccg gcgcttcttc gagcagcgca
      841 acctgccggt acggatcttc ccctcctacg gcgccaccga ggccaaggtg cccgagatcg
      901 tcgacgcgat cgtcgacctc accgagaccg gctcgaccct gcgcgcccac ggcatggtgg
      961 tgatcgccac cctgctgacc tcccgcaccg aggtgatcgc caaccccgcc gccgcgatcg
     1021 acccggagcg ccgccaggcc atggacgacc tggtgacgct gctggtcggg gccgccaccg
     1081 cccgctccaa gacactcatc aagctgaacg tcgccgccgc cgacctgccc gcggtgctgg
     1141 aggtgctgcc ggcgatgaag gcgccgacgg tgaccgcgct ggccggttca ggcttccacg
     1201 cggtcgagac ggtggtcgac aagagcggcg tgaaccggct gattccgacg ctgaaagccg
     1261 ctggcgcgtt cgacatcctg gagcttccga tcgcgaagat cgtcgcctga acgagcctcg
     1321 cagcttcgga caccctcgct gaccaggttg tttacgcttg tgaaagaccg tgcttgtgca
     1381 catgttgcgc gtgtattaca gtccgcccgc ctggccggga tgccccagca atcggtgtcg
     1441 ggacggccgg gggtcgacag gagggaagta cgttgccaga aggcaccgtc aaatggttca
     1501 gcaacgagaa gggctatggg ttcatcgccc ggaacggggg cgatgatgtc ttcgtgcact
     1561 tctcggccat ccagatcgac ggctacaaga ccctgagcga gggccaggct gtcgagttcg
     1621 agatcaccaa tgggcccaag gggccccagg cctcgaacgt ccggccgctc gcgtaggccg
     1681 cttgtcttgg gcgccggaga tcaaggactt ccggcaacgg ctcgctgaac aatggccccg
     1741 tcgctgtgac ggggccattt gccgtctcag ggctgctcgt cacggctggc ccggcaccca
     1801 caggcccgac ggcggacggt gcaggcggga gtcctcctcg ccctcgccac cttccggcgg
     1861 gggaggctcg cccggctgct gctcccggcg gagcagctcc gcgtagttca ggcgcaggct
     1921 cgccagggcc tgagccagct ccggctcgta ggcgccgagg cggccctcgg tcgcctccag
     1981 cagcccgccg aaggcgtcga tcagcaggcg ggccgcgtcc aggtcgcggt attccgcgtt
     2041 ctgctcggcg gggaggccga gccgcaggta agcgaaggtg accagctggg cggcggcctc
     2101 cgcgaccacg tcaccggccg agaccgcggt aagctgctcg cgcatctgct ggatctggtc
     2161 ggcgtcttca gtcatccaca ggactcctta cgcccgcgac gcttccgcga ccgggcatcg
     2221 acactgctgc gggaccactg ctacagtacc gtcatcgtat cgggcccgtt tcgccggtat
     2281 ggcgagaggc gccacgatca actgcataga ccgatagaag taggagcgag ggcttctcac
     2341 ctccaggccg tgaccgcaag gcgcgagtca gtgggggttt gtgacacccc cacggcatgg
     2401 ggtgagcggc ccttgggccg catttttcgt ctctggccac cggtggccac aaggagggat
     2461 gttgcatcag cgttgagccg agaatcaacg accgtatccg cgcggcgcag gtccgggtgg
     2521 tcggcgccga cggcgagcag ctcggggtga tggaaaccca ggtcgccctg cggatggcca
     2581 gcacctccga cctcgacctg gtcgaggtgg cgccgctggc caagccgccg gtcgtgaagc
     2641 tgatggacta ttcgaagttc aagtacgagc agggcatcaa ggccaaggag gcccgccgca
     2701 agcaaagctt ggtcgtgctc aaggaaatga agatgcggcc caagatcgac gaccacgact
     2761 acgaaaccaa gaagggccac gtcgagcgtt tcctcaagca gggccacaag gtcaaggtca
     2821 ccatcatgtt ccgcggccgg gagatggccc acaccgagct cgggcggcgg ctcctggagc
     2881 gtctcgccac cgacatcgtg gagatcgccc aggtcgacag ccccgcccgc caggacggcc
     2941 gcaacatgat cgcggtgttc tcgccccgca agaaccccgg cggcggcacc cccaagggcg
     3001 gccagtcgcc ccaggccgca ccccagacgc agggtccccc tccggggcag ggcgaccacg
     3061 gccctggcag caacgggcgg gcgccctcca cctcggccac cgcaacctga accatccggc
     3121 gccggcgagc agcccggcgg cgccgccacc gagcagcacc agaccgaacc gagggagaac
     3181 cccgacgtgc ccaagatgaa gacccaccgt ggcgccgcca gccgtttcgg cgtgaccaag
     3241 cgcggcaagc tggtccgccg ccacaccaag ctgaaccaca tcctggagaa gaagtccccg
     3301 ggcaccaagc gccgcctcgg ccgcgagggc gaagtcgcca agggcgaccg ccgcagcgtc
     3361 cgccgcatgc tcggcatctg aacgagatct gaactcgaag gaccgacaga cagaaacaga
     3421 aagagacagt ccaatggcca gagtgaagcg agcagtccac ggagccaagg ccaagcgcac
     3481 catcatggag caggccaagg gctaccggtc cagccgcgga cgccacttgc gggtggctaa
     3541 ggaacaggtg atgcactccc tgcggtacgc ctaccgcgac cgccgggccc gcaagggcga
     3601 gttccgctcg ctgtggatcg cccgcatcaa cgccgccgcc cgtgccgagg gcctcaccta
     3661 caaccgcttc atcgcgggcc tgcgcggcgc cggggtcgag gtcgaccgca aggtcctcgc
     3721 cgacatcgcc gtgaccgatc ccgccgcctt cgccgcgctg gtcgccaccg cccgggaggg
     3781 cctgaacgcg agcccgtgac cccaccgtgc ccgctcccgc cgccaccgtg aacgtgatca
     3841 ccagcgcccg gaaccccgcc gtgctggccg cccgcaagct cgcccgccag cgccgcgccc
     3901 gcgccaccga cgggcgcttc ctggtggagg gcgcccgcac gatccaagcg gccctcgacg
     3961 ccggccaggt cccagccgcg ctgctcgcca ccgaagcggc cgccgcccgc cacggcggcc
     4021 tgctcgcccg cgcccgccgc gccggcgccg gcgtcaccgt ggtcgcgccg ggcctgctcg
     4081 acggcatcgc ccagaccgtc accccccagg gcttggtcgc ggtcctggag cgcgtgaccc
     4141 gcccgctcgc cgcgctgccg ccggaccccc ggctggtctg cgtcctcgcc gaggtccgcg
     4201 accccggcaa cgccggggcg gtgctgcgcg ccgccgacgc cttcggcgcc gacgcgctgg
     4261 cgaccaccgc cgggtcggtc gacctggaaa gccccaaagc ggtccgcgcc gccgccggca
     4321 gcctgttcca cctgccggtg atcgccgatg cgccgtggcc cgagctggcg acccagcttc
     4381 gccgacgcgg cctgctgctc atcggcgccg acccgcacgc caccgccacc gtcgacgacg
     4441 caccgctgga gcggccctgc gcgctgatct tcggcaatga ggcccacggc ttggacgacg
     4501 agctgcggcg ctccctcgac ctgacagtcc gcgtgccaca cgacggccgc gccgaaagcc
     4561 tcaacctggc cgctgcggca gcggtgctgc tgtatgagac cgcccgccgg caacgccacg
     4621 cacgcccggc cccggacccg gccccggaac cggcccctca ggcggaggcg aaggccgagg
     4681 ccgacgccgc cgtcgccgag ctgatcacca cgctcgccca cgacctgcgc tccccgctga
     4741 ccggcatcaa gggcttcacc gccacactgc tgcaccgctg ggagcggttc agcgacgagc
     4801 agaagcagca catgctgcgc accatcagcg ccgacgccga ccggctcacc cgcctcatca
     4861 aagagctgct ggacgtcgcc cgcatcgagg ccgggaagct ggagttgcgc cgcgagatgg
     4921 tcgacctgcc cgccatcgcc gagggcgtgg ccggccggtt cgagccggcc gaccgtgggc
     4981 cccgcggcgc ccgcctcgca atcgtgtttc cggatggctt cccggacctc tatgctgacc
     5041 gtgacaagct cgaacaggtc ctgaccaacc tggtggagaa cgcggtgaac cacaccgagg
     5101 acggcacggt gaccatcgtc ggggaggtcg ccggcgacgc ggtcgaggtc gccgtgcgcg
     5161 acccgggttc aggcgtcccc gccgagcagc tcccgcaggt cttctccaag ttctggcgcc
     5221 gccccgaccc cctccgcggc gccccgcgtg gcagcggcct cggcctctac gtcgcctgtg
     5281 gcctggttga ggcccacggt ggcaggatct gggcgacctc gccgccctcg ggcggcctcg
     5341 agcttcgatt tcgcctgccc cgcgggggct tggagctcgc cggcatccac gagcaccagc
     5401 cggcaccgtc tcccagctga gcggggccga ccgccaagcg gacgaagcgc ggaccgccac
     5461 ccacgacgtc gcgccgcatg cgagcattgc gcctccacct ctaccgtcgg ttggaggcat
     5521 caaggagcca ggaccatgcc gaacgaagcc aacacaccac agcagccgag ccaaccagcc
     5581 accgacgttg acctcgacgc ggtcgtcaag gacggcctcg cggccatcgc cgccgccggc
     5641 gacctcgacg ccctccagca ggccgaccag gcccacctcc agcggcgctc cccgctcggc
     5701 gaggcgcagc gcagcctcgg ccgcctcgac gcggccgccc gtcgccagct cggccagcag
     5761 gtcaaccagg cccgcgccga gctgcaggcc gccctcgccg accgccgcgc cgagctggag
     5821 gccgcccgcg acgccgccct gctggaggcc gaacgcatcg acgtcaccct tcccggccgg
     5881 atcccgccgc gcggcgcggt ccacccgatc agccgcaccg ccgaggagat catcgacatc
     5941 ttcatcggcc tcggctaccg cgtcgccgaa ggtcccgagg tcgaaaccga ctggtacaac
     6001 ttcgaggcgc tgaacttccc gcccgaccat cccgcccgct ccatgcagga cagcctctcg
     6061 ctggaggcga ccgaccccaa cggcgtgccg ctgctgctgc gcacccacac ctcgccgatg
     6121 cagatccgca gcatgctcgc cgccccgccg ccgctgtacg tggtgatccc cggccgctgc
     6181 taccgcgccg acacccccga cgccacccac gtgccggtct tcaaccagat cgagggcctc
     6241 gccgtcgaca ccggcatcac catgggcgac ctcaaaggca ccctgctcgc cttcgcccgc
     6301 gcctacttcg gcgacgaccg gcaggtccgc ctgcggccgt cgtacttccc gttcgtcgag
     6361 cccggcgccg aagtcgacgt gtcctgcttt gtctgcggcg gccgcggcag cggctgccgg
     6421 acctgcaagg gcgaaggctg gatcgagctg ctcggcgccg gcatggtcca ccccaacgtg
     6481 ctgcgcgccg gcggctacga ccccgaccag gtctccggct tcgccttcgg catgggcatc
     6541 gagcggccct acatgctccg ctccggtctc gccgacatcc gcaccctcac cgacaacgac
     6601 ctgcgctggc tggccagcgt ctgagcgaac cgaacagaaa ggaggcagac ccatgcgcgt
     6661 accgctcacc tggctggccg tccacgtcga cctcggcgac gtctcagtcc aggccctggc
     6721 cgaccagctc accttcgccg gcctggagat cgagaccatc gactgggtcg gccaagacgt
     6781 cgccggggtg atcaccgccc gggtcctgcg gatcgccccc catcccgacg ccgaccggct
     6841 cgccctggtc accatcgacc cgggcccggg cggcgagccc cgcgaggtcg tctgcggcgc
     6901 ccacaactac gcccccggcg acgtggtccc ctgggccgcc cccggcgcca agctccccgg
     6961 cggcctcgag atcggccgcc gcaaggtccg cggccgctgg tcggacggga tgctcgcctc
     7021 cgcccgcgaa ctcggcgtct tcgacgacca ctccggcatc ctgatcctac cgcccgacac
     7081 ccccgtcggc gtcgacctga ccgaagagga aggcgtcggc ctgcgcgacg ccgtcttcga
     7141 catcaagacc ttccccaacc gcggcgacac cctctcgatc cgcggcgtcg cccgcgaggc
     7201 cgccctcgtc ctcggcaccg agctgaagcc gctcgacctg accgtccccg aacgcgggcc
     7261 ccaggctgcc acgctcgcca ccgtcgaggt ggacgacgcc gagggctgcc cgatctacta
     7321 cgcccgggtg atcgaaggcg tcgacgccgg ccgccccgcg ccgctgtgga tggcccgccg
     7381 cctctacctg tacgggcagc gacccctcgg cgcggtcgtc gacgtcacca actacctgct
     7441 gatcgaccag ggccagccgc tgcatgcctt cgacctggac cgcatcccca gccaccggat
     7501 cctggtgcgc cgcgcccagc ccggcgaagt gattcgcacc ctcgacggcc gcgaccgtca
     7561 cctgaccccc gaggacacgc tgatctccgc cggggacgag ccgctcgccc tggccggcat
     7621 catgggcggc gaggactccg aggtccgccc cgactcgacc cggatcctcc tggaggccgc
     7681 ccacttccca cccgccttcg tgcgccgcac cacccgccgc ctcggcatga gcaccgaagg
     7741 cgcgcagcgc tgggcccgcg gtgtcgaccc cgccggcgcc ggcccgatcg gcgaccaggc
     7801 cgcccgcctc atcgccgagc tggccggcgg caccgtcgcc accgggcgcc tccaggacgg
     7861 tcctggcgtc ccgccccgcg aggcggtccg cctcgactgg cgccgctccg cacagcggct
     7921 cggcgcgccc gccgaccccg ccttcgccgc cccctacctg gagggtgtcg gctgccaggt
     7981 ccagctcgaa cccgacagcg ccgccctgcg agcggtcccg ccgagctggc gcttcgacat
     8041 cgagggctgg gcggacctgg aggaggaggt cgcacgccgc tggggctacg acaagctccc
     8101 cgcgaccctg cccaaggcga ccggcgggcg ccgcaccgcc gagcagcgcc tgcgccgccg
     8161 cacccgcgac ctgctcgccg ggatgggtct caccgaggtg cagccctacc cgttcaccag
     8221 ccagggcgcc ctcgaccgcc tggagctgtc cgccagcgac cagcgccgcc gcgccctgcg
     8281 actcgccaac ccgctctcgg aggagtcccc ggagctgcgt accaccctgc tgcccggcct
     8341 cgccgaggcc gcccggcgca acctcgaaaa gggcctggcc ggcg
//
LOCUS       DASWDC010000307         4719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1417620, whole genome shotgun sequence.
ACCESSION   DASWDC010000307 DASWDC010000000
VERSION     DASWDC010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417620"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="VF995_09935"
     CDS             <1..1519
                     /locus_tag="VF995_09935"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HEX9343919.1"
                     /translation="LLEFLGGAVLVAHNAPFDHRFLQANLERHHYRRLTNRVVCTARL
                     ARKLLPRDEVPNVRLATLAAYLGATATPCHRAFTDARATVDVLHALLERAATFGVLTL
                     EDLLEFPTGRGSPTFRKVHLADALPHTPGVYLFRDSAARVLYVGKAKDLRARVRSYFS
                     GASDGRAKVADLLRELASIDHIPCATDFEASVREVRLIQQLRPRYNRRSRNPERYAYL
                     KLTRERFPRLSVVRRVLDDGARYLGPLSSAGQAELVKTAIEDALPLRRCTDRIGPRAR
                     VSSPCILLQLRRCLGPCQPDADEAAYAGVVALFEQALDGDPAPLLAPLRQRMAAYAAV
                     QRYEQAAAARDRLEALSRALGEARRRSSLSGVDELVVAQPHPDGREVVVLRRGQLAGV
                     AVLGAADDDGVARLREAAAQPERFEGPPPRHLADETALVSRWLDGVAGRAELLAVSGR
                     LASPARGGAALQVRYDPRRARGLTLPGAADGRPEPRPPRRPLRPRRALALAESRA"
     gene            1516..1869
                     /locus_tag="VF995_09940"
     CDS             1516..1869
                     /locus_tag="VF995_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX9343920.1"
                     /translation="MTQIPRKPDIAPVDFFAVDMRVGRVVEAEAFPEARKPAWKLTVD
                     FGPAVGTLRTSAQVTNYKREELLGRLVVGAVNLGTKRIAGFTSQFLILGGIDEAGTVR
                     LLIPDEGSQPGEPIA"
     gene            1933..3123
                     /locus_tag="VF995_09945"
     CDS             1933..3123
                     /locus_tag="VF995_09945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9343921.1"
                     /translation="MQQRNVVIVAAKRTPMGKFMGGLADVPAVDLGITAARAALEAAG
                     LAPEAIQETVAGHGRQAGNGPNPGRQVSVRTGIPETAPAYTVNKACGSSMKALALAAG
                     SILLGELDVALVVGMENMTCTPYLLRGMRGGYRMGDATVEDGMSSDGFMDPLSGIGMG
                     ETAENLAERYHVTREEQDAFALQSQRRASAAWAEGRFADEVVPVETTDRKGRPIGVER
                     DEQPRPDTTLEGLAALRPAFRKPGTVTAGNSSGICDGAAALVVMTEEAARARGIAPLA
                     RVVGWASAGVDPAIMGWGVVPATQKVLAKTGWPLPEIDVVEVNEAFAAQVLACDRELK
                     FDWERTNVNGGAIALGHPIGMTGARIVGAAVHELRRRGGQRALATLCISGGQGMAVAL
                     EAAD"
     gene            3147..3443
                     /locus_tag="VF995_09950"
     CDS             3147..3443
                     /locus_tag="VF995_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9343922.1"
                     /translation="MVTAIVLVTCGVAEIPETAQALADLDGISEVYSVSGEWDLIAIV
                     RVGNHDDLAELVTEHLRKVPGVKSTETLIAWRAYSRYDLDRLFSVGFESAGDPD"
     gene            complement(3490..4515)
                     /gene="trpD"
                     /locus_tag="VF995_09955"
     CDS             complement(3490..4515)
                     /gene="trpD"
                     /locus_tag="VF995_09955"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX9343923.1"
                     /translation="MFGRLLRGEDLPEADAAAAMREILAGEATSAQIAAFATGLRIKG
                     ETVTEITGLVHAMLEAATPLRVEVEGPLVDVVGTGGDRAGTFNVSTVAAIVVAGTGQR
                     VAKHGNRAASSRCGSADLLEALGVAISLPPEGVARTIAEAGIGFCFAPVFHPAMRHAA
                     GPRRELGVPTVFNFLGPLTNPARVRHQALGVSDPRMAPLMAEVLRRTGSRHALVFYGH
                     DGLDELTVTTTSTMLEVGPDSLRELVVDPEALGIAHPDLREVVGGDVERNLAIATQVL
                     AGEGGAPRDIVLLNAAAALVAADAVTSLPDGLEVAARSIDEGKAQATLDAWVEASQKA
                     QKAAQSN"
BASE COUNT          631 a         1755 c         1701 g          632 t
ORIGIN      
        1 cctgctcgaa ttcctcggcg gcgccgtgct ggtcgcccac aacgccccct tcgaccatcg
       61 cttcctgcag gccaatctgg aacgccacca ctaccgccgc ctcaccaacc gggtggtgtg
      121 caccgcccgg ctcgcccgca agctcctgcc ccgcgacgag gtcccgaatg tgcgcctggc
      181 gaccctcgcc gcctacctcg gcgccaccgc gacgccctgc caccgcgcct tcaccgacgc
      241 gcgcgccacc gtcgacgtgc tccacgcact gctggaacgg gccgcgacct tcggcgtgct
      301 caccctcgaa gacctgctcg agttccccac cggccggggc agccccacct tcaggaaagt
      361 ccacctcgcc gacgcgctcc cccacacccc cggcgtctac ctgttccgtg acagcgccgc
      421 ccgggtcctc tacgtcggca aggccaagga cctgcgcgcc cgcgtccgca gctacttctc
      481 cggcgccagc gacggccgcg ccaaggtcgc cgacctgctg cgcgaactgg ccagcatcga
      541 ccacatcccc tgcgccaccg acttcgaggc cagcgtccgc gaggtccgcc tgatccagca
      601 gctccgcccc cgctacaacc gccgcagccg aaatcccgag cgctacgcct atttgaagct
      661 cacccgcgag cggttccccc ggctgtcggt ggtgcgccgg gtgctggacg acggtgcacg
      721 ctatctcggg ccgctctcgt cggccggcca ggcggaactg gtcaaaaccg cgatcgagga
      781 cgcactgccg ctgcgccgct gcaccgaccg gatcgggccg cgtgcccgtg tctcgtcgcc
      841 ctgcatcctg ctgcagctgc gtcgctgcct cggtccctgc cagccggacg ccgacgaagc
      901 cgcctacgcc ggtgtggtgg cgctgttcga gcaggccctg gacggcgacc ctgcgccgct
      961 gctggcgccg ttgcggcagc ggatggccgc ctatgccgcc gtgcagcgct acgaacaggc
     1021 ggcagcggcc cgcgaccggc tggaggcgct gtcccgcgcc ctcggcgagg cccgccgccg
     1081 cagcagcctc agcggcgtgg acgaactggt cgtcgcgcaa ccccatcccg acggccgcga
     1141 ggtggtggtg ctgcgccgcg gtcagctcgc cggcgtggcc gtcctgggcg ccgccgacga
     1201 cgacggggtc gcgcggctgc gggaggcggc ggcccagccg gaacggttcg aggggccgcc
     1261 gccacgccac ctcgccgatg agacggcgct ggtcagccgc tggctcgacg gtgtcgccgg
     1321 ccgcgccgag ctgctggcgg tcagcggccg cctggccagc cccgcccgcg gcggcgccgc
     1381 gctccaggtc cgctatgacc cgcgccgcgc ccgcgggctg accctcccgg gcgcggcgga
     1441 cggccggccc gagccgcgcc cgccacgccg cccactgcgt ccgcggcgcg ctctggctct
     1501 ggcagaatcc agggcatgac tcagatcccc agaaaaccgg acattgcccc agtggacttc
     1561 ttcgctgtgg acatgcgcgt cggccgggtc gtcgaggcgg aggcgttccc cgaggcccgc
     1621 aagcccgcct ggaagctcac cgtcgacttc ggccccgccg tcggcaccct gcgcaccagc
     1681 gcccaggtca ccaactacaa gcgcgaggag ctgctcggcc gcctggtggt cggtgcggtc
     1741 aacctcggca ccaagcgtat cgccggcttc accagccagt tcctgatcct cggcggcatc
     1801 gacgaagccg gcacggtgcg cctgctcatt cctgacgagg gttcccagcc gggcgaaccg
     1861 atcgcctgac cggtggggat gccggccgcc actacaatga gcctcggctc gttcagcggc
     1921 tggaggcagc agatgcagca gcgaaatgtc gtcatcgtcg cggccaagcg gaccccgatg
     1981 gggaagttca tgggggggct ggccgacgtc ccagcggtcg atctcgggat cacggcggcc
     2041 cgtgccgcac tggaggccgc cgggctcgcg ccggaagcta tccaggagac cgtggccggg
     2101 cacggccggc aggccggcaa cgggccgaac ccgggccggc aggtctcggt ccgcaccggg
     2161 atccccgaga cggctcccgc ctacaccgtg aacaaggcgt gcgggtcgag catgaaggcg
     2221 ctcgccctgg ccgccgggtc gatcctgctc ggcgagctgg acgtcgcctt ggtggtcggg
     2281 atggagaaca tgacctgcac cccctacctg ctgcgcggga tgcgcggcgg gtaccgcatg
     2341 ggcgacgcga ccgtcgagga cggcatgtcc tcggacgggt tcatggaccc cctgtcgggc
     2401 atcggcatgg gcgagaccgc cgagaacctc gccgagcgct accacgtcac ccgcgaggag
     2461 caggacgcgt tcgcgctgca gtcgcagcgg cgcgccagcg ccgcctgggc ggagggccgc
     2521 ttcgccgacg aggtcgtccc ggtcgagacc accgaccgca agggccgccc gatcggggtg
     2581 gagcgcgacg agcagccccg ccccgacacc accctggagg gtctggccgc cctcaggccg
     2641 gcgttccgca agcccggaac ggtcaccgcc ggcaactcct ccgggatctg cgacggcgcg
     2701 gcagcactgg tggtgatgac cgaggaggcc gcccgtgcgc gcggaatcgc ccccctggcc
     2761 cgggtggtcg gctgggccag cgccggggtg gacccggcga tcatgggctg gggggtcgtc
     2821 ccggccactc agaaggtgct cgccaagacg ggctggccgc tcccggagat cgacgtggtc
     2881 gaagtcaacg aggcgttcgc cgcccaggtc ctcgcctgcg accgggagct gaagttcgac
     2941 tgggaacgga ccaacgtcaa cggcggcgcg atcgcgctcg gccacccgat cggcatgacc
     3001 ggcgcgcgga tcgtcggcgc cgccgtccac gaactgcgcc gccgcggtgg ccagcgtgcc
     3061 ctggccaccc tgtgcatctc cggcggccag ggcatggccg tcgccctgga agccgccgac
     3121 tgattccctg aagggagctt gaggccatgg tcaccgcgat cgtgctcgtc acctgcgggg
     3181 tcgccgagat ccccgagacc gcccaggcgc tcgccgacct cgacggaatc agcgaggtct
     3241 actcggtctc cggggagtgg gacctgatcg cgatcgtccg cgtcggcaac cacgacgacc
     3301 tcgccgagct cgtcaccgaa cacctccgca aggtccccgg cgtgaagtcc accgagacgc
     3361 tgatcgcctg gcgtgcctac tcccgctacg acctcgaccg cctcttctcc gtcggcttcg
     3421 aatccgccgg cgacccggac tgaccgcgtc ttccgcttcg gcgccgactt ctgcgcggct
     3481 ccgaaagcgt cagttcgact gggctgcctt ctgggccttc tgggaggctt cgacccaggc
     3541 gtcgagggtg gcctgggcct tgccctcgtc gatcgagcgg gcggcgactt cgaggccgtc
     3601 ggggaggctg gtgacggcgt cggcggcgac cagggcggcg gcggcgttga gcaggacgat
     3661 gtcacgggga gcgccgccct cgccggcgag gacctgggtg gcgatggcga ggttgcgctc
     3721 gacgtccccg ccgacgacct ctcgcaggtc ggggtgggcg atgcccaggg cttcggggtc
     3781 gacgaccagc tcgcggaggc tgtcggggcc gacctccagc atggtggagg tggtggtgac
     3841 ggtcagctcg tcgaggccgt cgtggccgta gaagaccagg gcgtggcggc tgccggtgcg
     3901 gcgcaggacc tcggccatca ggggggccat gcgcgggtcg gagacgccga gggcctggtg
     3961 ccggacgcgc gccgggttgg tgagcgggcc gaggaagttg aacaccgtgg gcacgccgag
     4021 ctcgcggcgg ggcccggccg cgtggcgcat ggccgggtgg aagaccgggg cgaagcagaa
     4081 gccgatgccg gcctcggcga tggtccgggc cacgccctcg ggaggcaggc tgatcgccac
     4141 gcccagggcc tccagcaggt cggcgctgcc gcagcggctg gaggcggcgc ggttgccgtg
     4201 cttggcgacg cgctggcccg tcccggcgac gacgatcgcg gccacggtgg agacgttgaa
     4261 ggtgccggcc cggtcgccgc cggtgccgac cacgtcgacc agcgggccct cgacctcgac
     4321 ccgcagcggg gtggccgcct cgagcatggc gtggaccagg ccggtgatct cggtgacggt
     4381 ctcgcccttg atgcgcagcc cggtggcgaa ggcggcgatc tgcgcggagg tcgcctcgcc
     4441 ggcgaggatc tcgcgcatgg ccgcggcggc gtcggcctca ggcaggtcct cgccgcgcag
     4501 cagccgcccg aacaccgcgg gccatgagaa cggtgacgat gcctgcggtg gctgcgaaga
     4561 ctggctggac actgctgcgc tcccggtcgg attctccggc ttctcccgcc ggacagccta
     4621 accgatatcg gcgcgtgcgc tgggttcagg caggctgcgt ctcagtgccg agggcgtcgc
     4681 ggagcgggtc gtggatcgcg cctgggtcga ggcggagcc
//
LOCUS       DASWDC010000308         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_739125, whole genome shotgun sequence.
ACCESSION   DASWDC010000308 DASWDC010000000
VERSION     DASWDC010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739125"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="VF995_09960"
     CDS             <1..1605
                     /locus_tag="VF995_09960"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX9343924.1"
                     /translation="GRNCAVIEYAGARLLIDCGLAFPDADQPGVDIILPDFTYLAEER
                     ERVVGVVLTHAHEDHMGALPYFLRDFDVPVYGTRLSLGMLSAKLEEHDLAPSLVEVGA
                     GERTTLGPFELEFFAVSHSIPDGVAVAVRTPAGTIVHTGDFKMDLTPIDHRPTDLGGL
                     ARLAGEGIDLLLADSTNAEQPGFIPPEAEVGRALTDIFAAAKRRIVVACFASHVHRMQ
                     QILGAAEQSGRKVALVGRSMLRNIRVAHELGYLRVPPGLLIPVEDLIAYPPEQTVVLC
                     TGSQGEPFSALALMAARDHKWVRIEPGDTVVLSSSLIPGNEAAVYRTINDLARQGAEV
                     FHRGNAKVHVSGHAAQGELTLLLATLRPRQFTPVHGEYRHLASHARLAEKTGISRDRI
                     FICQDGDVLELDAGRVRRGGEVRAGMVFVDGLGIGDVGDAVLRDRRKLGEEGFVHIVA
                     TVEAQTGKVVAGPDIVTRGFVYEPESADLIDEARARVLASLDRAAREGVSDPMILKQA
                     MREAASSLFRERTHRRPVIVPTVMEV"
     gene            1608..>2557
                     /locus_tag="VF995_09965"
     CDS             1608..>2557
                     /locus_tag="VF995_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX9343925.1"
                     /translation="MARTRTRRPPSKRPTASKKRPSPPSQTKRNKPATPTALAPHKGD
                     IQGVALILIAVLLALGVWLHNGGKVGLAAELALRGLFGYGAYAVPMLVAGAGASLFRR
                     SVAPRGEPVASRAAIGLVAVLLGGLGLVDLLRGAPTATSSTAVLQNSGGWVGAVIGTP
                     LARLLSTWGAGVGFACLLFLGLLISTRTSVASVMRGLGAVARMIRSAVTPPPRPAGDA
                     AAAGSAADGASGKGAAKADGASGAGGAGNDNGKGARGRASATPAAAGEAAATAMDDPA
                     GALEEPRRSRRGRKAAAAAATPPSDEFEGETVEVNRAAPPP"
BASE COUNT          338 a         1014 c          876 g          329 t
ORIGIN      
        1 gggcgcaact gcgccgtcat cgagtatgcc ggcgcgcgcc tgctgatcga ctgcggcctc
       61 gccttccccg acgccgacca gccaggcgtc gacatcatcc tgcccgactt cacctacctc
      121 gcagaggagc gcgagcgggt cgtcggcgtc gtgctgaccc atgcccacga ggaccacatg
      181 ggcgcgttgc cgtacttcct gcgcgacttc gacgtgccgg tctacggcac ccgcctgtcg
      241 ctcggcatgc tctccgccaa gctggaggag cacgacctcg ccccctcgct ggtcgaggtc
      301 ggcgccggcg agcgcaccac cctcggcccc ttcgagcttg agttcttcgc ggtgtcgcac
      361 tcgatccccg acggggtcgc ggtggcggtc cgcaccccgg ccgggacgat tgtgcacacc
      421 ggtgacttca agatggacct gaccccgatc gaccaccgcc cgaccgacct cggtggcctc
      481 gcgcggctgg cgggggaggg gatcgacctg ctgctcgccg actccaccaa cgccgagcag
      541 cccggcttca tcccccccga ggccgaggtc ggccgcgccc tcaccgacat cttcgcggcg
      601 gcgaagcggc gcatcgtggt cgcctgcttc gccagccacg tccaccgcat gcagcagatc
      661 ctcggcgccg ccgagcagag cggccgcaag gtcgccctgg tcgggcgcag catgctccgc
      721 aacatccgcg tcgcccacga gctcggttac ctgcgggtcc cgcccggcct cctgatcccg
      781 gtcgaggacc tgatcgccta cccgccggag cagacggtgg tgctgtgcac gggttcgcag
      841 ggcgagccgt tctcggcgct ggcgttgatg gccgcccgcg accacaaatg ggtgcgcatc
      901 gagccgggcg acaccgtggt gctgtcctcc tcgctgatcc ccggcaacga ggcggcggtc
      961 taccgcacca tcaacgacct cgcccgccag ggcgccgagg tcttccaccg cggcaacgcc
     1021 aaggtccacg tctccggcca cgccgcccag ggtgagctga ccctgctgct cgccaccctg
     1081 cgcccacgcc agttcacccc ggtccacggc gagtaccgcc acctcgccag ccacgcccgc
     1141 ctggccgaaa agaccggcat ctcccgcgac cggatcttca tctgccagga cggcgacgtg
     1201 ctggaactcg acgccggtcg cgtgcggcgc ggcggtgagg tccgcgccgg catggtgttc
     1261 gtcgacgggc tcggcatcgg cgacgtcggc gacgcggtcc tgcgcgaccg ccgcaagctc
     1321 ggcgaggaag gcttcgtcca catcgtcgcc acggtcgaag cccaaaccgg caaggtggtc
     1381 gccggccccg acatcgtcac ccgcggcttc gtctacgaac cggaatccgc cgacctgatc
     1441 gacgaggccc gcgcccgcgt gctcgccagc ctcgaccgtg ccgcccgcga aggcgtcagc
     1501 gacccgatga tcctcaagca ggccatgcgg gaggctgcct cctcgctgtt ccgcgagcga
     1561 acccatcgcc gccccgtgat cgtgccgacc gtcatggagg tctgaaggtg gccaggaccc
     1621 gcacccgccg gccgccgagc aagcggccga cggcctccaa gaaacggccc agcccgccgt
     1681 cgcagaccaa gcgcaacaag cccgccacgc ccaccgcgct ggcgccgcac aagggcgaca
     1741 tccagggcgt cgcgctgatc ctgatcgccg tgctgctggc gctcggcgtc tggctccaca
     1801 acggcggcaa ggtcggcctc gccgccgagc tggccctgcg cggcctgttc ggttacggcg
     1861 cctacgccgt cccgatgctg gtcgcggggg caggggcgtc gctgttccgc cgttcggtcg
     1921 ccccccgcgg cgagccggtc gccagccggg ccgccatcgg gctggtcgcc gtgctgctcg
     1981 gcgggctcgg cctggtcgac ctgctgcgcg gcgcgccgac agcgacctcc tccaccgcgg
     2041 tgctgcagaa ctccggcggg tgggtcggtg cggtgatcgg caccccgctc gcgcggctgc
     2101 tgtccacctg gggcgcgggg gtcgggttcg cctgcctgct gttcctcggg ctgctgatca
     2161 gcacccgtac ctcggtcgca tcggtgatgc ggggcctggg ggccgtcgcg cggatgatca
     2221 ggtcggcggt caccccgccg ccgcggcccg ccggcgacgc cgccgcggcc ggaagcgccg
     2281 ccgacggcgc cagcgggaag ggcgccgcca aggccgacgg cgccagcggc gccggcggcg
     2341 ccgggaacga caacgggaag ggcgccaggg gccgcgccag cgccaccccc gcagccgccg
     2401 gcgaagccgc cgcaaccgcc atggacgacc cggccggggc gctcgaagag ccgcgccgct
     2461 cgcgccgcgg ccgcaaggcg gctgccgcgg cggccacacc cccgagcgac gagttcgagg
     2521 gcgagaccgt cgaggtcaac cgcgccgcgc cgccccc
//
LOCUS       DASWDC010000309         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_461518, whole genome shotgun sequence.
ACCESSION   DASWDC010000309 DASWDC010000000
VERSION     DASWDC010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461518"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /gene="selD"
                     /locus_tag="VF995_09970"
     CDS             <1..567
                     /gene="selD"
                     /locus_tag="VF995_09970"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX9343926.1"
                     /translation="AARPGDQLVLTKPLGTGVVATAIKRGEPDPSWVEAAVASMTTLN
                     RSAAEAAVAAGVRAATDVTGFGLLGHLHRLLRASGVAAEVDAARVPFLPGAAELAADG
                     FVSGGTRNNQAFLADKVELEAGVPPVVATLLFDAQTSGGLLLAVPPERLRALLEELRD
                     HGGYPAALVGRVLAGPPGRIRVRRLPPE"
     gene            640..1068
                     /locus_tag="VF995_09975"
     CDS             640..1068
                     /locus_tag="VF995_09975"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX9343927.1"
                     /translation="MFRLDQQDYDALVAHARVEYPNEACALLGGRDHTVERVYALPNA
                     EASPTFYVVEPKAQLHAMNELDDLGLDLVGIFHSHTFTEAYPSRTDIQLAAYEDAAYL
                     ILSLADLDAPVLRAFRIRRGKVGEEPVTLVTRTAGAGEVR"
     gene            1310..1585
                     /locus_tag="VF995_09980"
     CDS             1310..1585
                     /locus_tag="VF995_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018816535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX9343928.1"
                     /translation="MPVEVRIPTILRKYSDGEKSVQAEGATVRGLISDLDRRYPGISA
                     QLLTDDGKLHRFVNVYVNDEDVRFLGALDAPLGEGDVVAILPAVAGG"
     gene            1615..>1944
                     /locus_tag="VF995_09985"
     CDS             1615..>1944
                     /locus_tag="VF995_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9343929.1"
                     /translation="MRYEDLVDAVGNTPLVGLPRLAPRPEVRLWAKLEGQNPTGSVKD
                     RIAKAMIDDAEKSGRLQPGNGATLLEPSSGNTGIALALVARLRGYRLVVVMPENTSEE
                     RRQLLRLY"
BASE COUNT          271 a          739 c          674 g          260 t
ORIGIN      
        1 gccgcccggc ccggggacca gctggtgctc accaagccgc tcggcaccgg cgtggtcgcc
       61 accgccatca agcgcgggga gcccgacccc agctgggtgg aggcggcggt cgcctcgatg
      121 accaccctga accggtccgc cgccgaggct gccgtggccg ccggggtgcg ggccgcgacc
      181 gacgtgaccg gcttcggcct gctcggccac ctgcaccggc tgctgcgtgc cagcggggtc
      241 gccgccgagg tggacgccgc ccgggtgccg ttcctgccgg gcgccgcgga actggccgcc
      301 gacgggttcg tctccggcgg gacccgcaac aaccaggcgt tcctggcaga caaggtggag
      361 ctggaggccg gcgtgccgcc ggtggtggcg accctgctgt tcgatgccca gacctccggc
      421 gggctgctgc tggcggtccc gcccgagcgg ctgcgggcgc tgctggagga gctgcgcgac
      481 cacggcggct acccggccgc gctggtcggc cgggtcctcg ccggaccgcc tgggcgcatc
      541 cgggtccgcc gcctcccgcc ggaatagtgg gcattgggtc ggcgttgagg ctgtggacag
      601 ccaatgtcgc gcccggggcg ccgatgctac gctcgactca tgttcagact tgaccagcag
      661 gactacgacg ccttggtggc gcacgcccgc gtcgagtatc ccaacgaggc gtgcgccctg
      721 ctgggcggcc gtgaccacac cgtcgaacgg gtctacgccc tccccaacgc cgaggcgagc
      781 ccgaccttct atgtggtcga accgaaggcc cagctccacg ccatgaacga gctggacgac
      841 ctgggcctcg acctggtggg catcttccac tcccacacct tcaccgaggc ctacccctcg
      901 cggaccgaca tccagctcgc cgcctatgag gacgccgcct acctgatcct gtccctcgcc
      961 gacctggacg ccccggtgct gcgcgccttc cgcatccgca ggggcaaggt cggcgaggag
     1021 ccggtcaccc tggtcacccg caccgcaggc gcaggcgagg tgcggtgacg gtgcgcatcc
     1081 tcgccttcac agggcccacc tgcgggctcg ccctcccgac ctggcaccgg cagggcccgg
     1141 ccgctcgcta cgggcgatgt cgcgcccgct gaccggcggg ctcccgagct gatcctggcc
     1201 tgagcgccgc cggccccggt ccgcggatcc gcgaacccgg cgctgccccc gcacgcgggc
     1261 gccgcccgct cccaaagtct gcatcacgtc aggaggttga cccaccacaa tgcccgttga
     1321 agtccgtatc ccgacgatcc ttcggaaata ttcggatggc gagaaatccg tccaagcgga
     1381 aggcgccacc gtccgcggcc tgatctccga cctggaccgg cgctacccgg ggatctccgc
     1441 ccagctgctc accgacgacg gcaagctgca ccgcttcgtc aacgtctacg tcaacgacga
     1501 ggacgtccgc ttcctcggcg cgctcgacgc cccgttgggc gaaggcgacg tggtcgcgat
     1561 cctgcctgcc gtcgccggcg gctgaacggc ggcaagcggg gaagaggagc ggtcatgcgc
     1621 tacgaggacc tggtcgacgc ggtcggcaac acccccctgg tcgggctgcc gcgcctggcg
     1681 ccgcggccgg aggtgcgcct gtgggcgaaa ctggagggcc agaaccccac cggcagcgtc
     1741 aaggaccgca tcgccaaggc gatgatcgac gacgccgaga agagcggccg cctgcagcct
     1801 gggaacggcg cgaccctgct ggagccctcc agcggcaaca ccggcatcgc gctcgcgctg
     1861 gtcgcgcggc tgcgcggcta ccgcctggtg gtggtgatgc ccgagaacac ctccgaggag
     1921 cgccgccagc tgctgcggct ctac
//
LOCUS       DASWDC010000310         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_127181, whole genome shotgun sequence.
ACCESSION   DASWDC010000310 DASWDC010000000
VERSION     DASWDC010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127181"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1797
                     /gene="cimA"
                     /locus_tag="VF995_09990"
     CDS             67..1797
                     /gene="cimA"
                     /locus_tag="VF995_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX9343930.1"
                     /translation="MNPSLDVPEHVETYDTTLRDGSQQVGLNFTVADKLRVTQLLDDL
                     GVDTIEGGWPGSNHTDAEYFQRVHDQPLRHATLAAFGATRRPRISVEGDDNLRALLDS
                     KAPVLTLVGKSWTLHVTEALRTTYEENLAMVRESVAYLTAAGRRVAFDAEHFFDGYAS
                     DHAYALAVADAAASAGADTVVLCDTNGGTLPGAVTQVVEQVVAALPGVRVGVHFHNDS
                     ACAVANSLVAVAAGARHVQGAANGYGERCGNADLFAMIAGLELKEGRELLPPGRLAAL
                     TTTAHAVAELANLTPDPRQPYVGATAFATKAGLHASAIARRPDAYSHIDPTTVGNRAR
                     VLVSELAGRSNVVAKARELGLDLNSSGNGDGNGANGNSGEDLGARVLDRVKELEHRGY
                     AFEVADASFELLVRREAGAFAAPFALEGYRVMVERREGPVSSGGPAATMGTSRGASGR
                     GTAPPEPPGDMAEATIRLLVGGDRVVAVGEGVGPVHALDQALRRALAEHHPELDRVHL
                     VDFKVRILDGREGTKAVTRVLITSADPAGEWTTVGVSDDIVTASWEALADGIQYGLLR
                     AAAPSPVTAS"
BASE COUNT          312 a          861 c          659 g          287 t
ORIGIN      
        1 gcgccggcgg ccagtccccg ccgccggcac ccactgccat acgcctgacc ggagggaagc
       61 aagcacgtga acccatcgct tgacgttcca gaacacgtag agacctacga caccaccctg
      121 cgggacgggt cgcagcaggt cggcctcaac ttcaccgtgg ccgacaagct gcgcgtcacc
      181 cagctcctcg acgatctcgg cgtcgacacc atcgagggcg gctggcccgg ctcaaaccac
      241 accgacgccg aatacttcca gcgcgtccac gaccagccgc tccgccacgc caccctggcc
      301 gcgttcggcg ccacccgccg gccgcggatc agcgtcgagg gcgacgacaa cctgcgcgcc
      361 ctcctcgact ccaaggcgcc ggtgctcacc ctcgtcggca agtcgtggac cctgcacgtc
      421 accgaggcgc tgcgcaccac ctacgaggag aacctcgcga tggtgcgcga gtcggtcgcc
      481 tacctgaccg ccgccgggcg ccgcgtcgcc ttcgacgccg aacacttctt cgacggctat
      541 gccagcgacc acgcctacgc cctcgccgtc gccgatgccg ccgccagcgc gggtgcagac
      601 accgtggtgc tgtgcgacac caacggcggt accctgcccg gcgccgtcac ccaggtggtc
      661 gagcaggtcg tggccgccct gcccggcgtc cgcgtcggcg tccacttcca caacgactcg
      721 gcctgcgcgg tcgccaactc cctggtcgcc gtcgccgccg gcgcccgcca cgtgcagggc
      781 gccgccaacg gctacggcga gcgctgcggc aacgccgacc tgttcgccat gatcgccggc
      841 ctcgaattga aggagggccg ggagctgctg ccacccggcc gcctcgccgc cctcaccacc
      901 accgcccacg ccgtcgccga gctggccaac ctgacacccg acccgcgcca gccctacgtc
      961 ggcgccaccg ccttcgccac caaggccggc ctgcacgcct ccgccatcgc ccgccggccc
     1021 gacgcctatt cacacatcga ccccaccacc gtcggcaacc gcgcccgcgt cctggtcagc
     1081 gagctggcgg ggcgctccaa cgtcgtcgcc aaggcccgcg agctcggcct cgacctgaac
     1141 agcagcggca acggcgacgg caacggcgcc aacgggaaca gcggcgagga cctcggcgcc
     1201 cgcgtcctcg accgtgtcaa ggaactggag caccgcggct acgccttcga ggtcgccgac
     1261 gcctccttcg agctgctggt ccgccgcgag gccggagcct tcgcggcgcc cttcgccctg
     1321 gagggctacc gcgtcatggt cgagcgccgc gagggccccg tctcatcggg gggccccgcg
     1381 gccaccatgg gaacctctcg cggtgcttca ggccgcggga ccgcgccccc cgaacccccc
     1441 ggcgacatgg ccgaagccac catccgcctg cttgtcggcg gtgaccgcgt cgtcgccgtc
     1501 ggggagggcg tcgggccggt ccacgccctc gaccaagcgc tgcgccgcgc cctggccgag
     1561 caccaccccg agctggatcg cgtccacctg gttgacttca aggtccgcat cctcgacggc
     1621 cgcgagggca ccaaggccgt caccagggtg ctgatcacct ccgccgatcc ggcgggggag
     1681 tggaccaccg tgggcgtctc cgacgacatc gtcaccgcct cctgggaggc gctcgccgac
     1741 ggcatccagt acggtctgct ccgcgccgcc gccccctcgc cggtcacggc ctcgtagccc
     1801 ggcgggcggg tccaatcggc gcccccctgt agccggatcg ttaggaaaac gcgtcttgcc
     1861 agatgtccta ttgggttaga ttgcctggca gaccggtttc tacatggttc aacgttccat
     1921 ggctccagcg gggggagatg ctgaaactgg ctgcaatgga aagtccgtcg tcgctgtccc
     1981 taacggcgcg tctcgctatt cgctgcgcgt tcctgggagc tgccgcgttc gtgatcctgc
     2041 tgctcgctgc cacccgcagc gaggctgcca ccaggaccga cacccggccg ccgcactccc
     2101 cagtcccggt ctccaagac
//
LOCUS       DASWDC010000311         5356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_740395, whole genome shotgun sequence.
ACCESSION   DASWDC010000311 DASWDC010000000
VERSION     DASWDC010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740395"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VF995_09995"
     CDS             <1..180
                     /locus_tag="VF995_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343931.1"
                     /translation="LEQDGRIMPGAFATTLHQFSNQLHQYQMQPHQITIPERPPIVTL
                     DVYRRRREAQPAGQG"
     gene            186..1079
                     /locus_tag="VF995_10000"
     CDS             186..1079
                     /locus_tag="VF995_10000"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX9343932.1"
                     /translation="MGGIPLAEAGAALVRLDPAVLFSDVDGTLVGRDGSLFADLDGGL
                     TLAAAEALVAAKAGGLDVVLVSGRNEAQLREANRLLGLRDAIGELGTVLVLGRQVELL
                     WGATPRELGATPHEALERSGATDLVLTTFGRSIEPHAPWHLGRVGTALLRGRVNPAEV
                     EAVLAAAGMGWVRLLDNGRLRGAYPQLSGEGPAHAYHLLPAGVSKAAAAAAYLERRGL
                     RASQAAAIGDSPADLGLAGSVGAAFLVQNGAWAAAADTGEAGNGADTGTPVIVTPSSA
                     GQGFAEAVHALLDHRKRAEPG"
     gene            1145..2602
                     /locus_tag="VF995_10005"
     CDS             1145..2602
                     /locus_tag="VF995_10005"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX9343933.1"
                     /translation="MAELVVASNRGPVQFEIDDEGTLTCGRGAGGMVAALGPALAGQG
                     GTWAAAAITEGDRVAARQVARSGRVRQVQLPQGPIRLRSLVIDPRQYQNYYNRVSNRT
                     LWFLQHYLFDAPRHPRFDQGFRTAWRDYREVNQAFAAACDSEASQGAQVFVQDYHLAL
                     TPALLRARRPELHIAHFTHCPWADPGYFTMLPSTTAYEFVEGMLGADLIGFLVPRWAR
                     NFLRCCEELGFEVNRRAQTVRAPDGRDARVRAYPLGVDDGDLRARVAEPDVQAQRRSL
                     RDLARGRSLIVRVDRMELSKNILRGLDGYTMLLEQNPRLRGRVVHFALAYASRRDLPE
                     YQAYTAEVRHAVDAINERFRTRSWEPVRLELRDNYPRALAAMAEADVLLVNPVWDGMN
                     LVAKEGMAVSERDAVLVLSRNAGAADDLAEGALLVNPFDTAELAASLASAVTMPAADR
                     AKRAALLRTQASALPPQDWFAAQRNDLERMGRTRS"
     gene            complement(2599..3162)
                     /locus_tag="VF995_10010"
     CDS             complement(2599..3162)
                     /locus_tag="VF995_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX9343934.1"
                     /translation="MRPGPTARAAAVFAVFLAVFIAVDQLSRAQPHRAAKATASVSSA
                     PAATSTTLGGLLGAPPTSAPAPAATTTSMVPPSSEPATTASVSTTTLPRVAGVTVQVL
                     NGVFVPGLAHQVADKLRAAGYDVVATQTAFGHFTVSRIYYTEGHQADALALQARFPAF
                     KVIAPAPSSLSRDIALHVVIGGNYKDI"
     gene            complement(3209..3433)
                     /locus_tag="VF995_10015"
     CDS             complement(3209..3433)
                     /locus_tag="VF995_10015"
                     /inference="COORDINATES: protein motif:HMM:NF023092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HEX9343935.1"
                     /translation="MATERDLSVLAFERRWWKHAGAKEEAIRREFGLHPTAYYQLLSR
                     VIDDPAALAAEPALVGQLREQRASRHRQRR"
     gene            3925..4863
                     /locus_tag="VF995_10020"
     CDS             3925..4863
                     /locus_tag="VF995_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX9343936.1"
                     /translation="MTTLSLTIQNGAQAAPTRTAQLKAQLDKLNQRADQLVEAYDQAN
                     AGLARTRGLLGSLKTDEADAGQTLQGLQQRVGERAAVAYMEGPGNALAELLNGSNPAD
                     TIDRMQILNLLAQQDGDLAAALRVAGRNFGARRAALVAAEKGQAAEVDALAGKKAAAE
                     AAVAKTQRLLNSLQAADRTALLGAVNGASSGSLPPITASGAVAKVLRYAEAQVGKPYA
                     FGAAGPSAFDCSGLTMMAWAQAGVSLPHTAAGQYDVISHVSTSQIRPGDLLFYYSPIS
                     HVAIYVGNGMQIAATHTGDFVRLQAIHSGLVGAGRP"
     gene            complement(4996..>5356)
                     /locus_tag="VF995_10025"
     CDS             complement(4996..>5356)
                     /locus_tag="VF995_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HEX9343937.1"
                     /translation="VIRSDNVDRVKARIVVEGANGPTTPSADKVLDDNDCLVVPDILA
                     NAGGVTVSYFEWVQDLQAYFWSGEDVTTRMRAVMERAYEEVTALAQERKLSMRQAAQV
                     IGVGRVAEAHRTRGLFP"
BASE COUNT          783 a         1975 c         1886 g          712 t
ORIGIN      
        1 ctggagcagg acggacgcat catgccgggc gccttcgcca ccaccctgca ccagttctcc
       61 aaccagcttc accagtacca gatgcagccg caccagatca ccatcccgga gcgcccgccg
      121 atcgtcaccc tggacgtcta ccgccgccgg cgcgaagccc agcccgccgg gcaggggtga
      181 ggcgcatggg tgggatcccg ctcgccgagg ccggcgcggc gctcgtccgc ctcgacccgg
      241 cggtgctgtt cagcgacgtc gacgggaccc tggtcggccg ggacggctcg ctgttcgccg
      301 acctggacgg tgggctcacc ctggcggcgg ccgaggcgct ggtcgccgcc aaggccggcg
      361 ggctcgacgt ggtgctggtg tccggccgca acgaggcgca gctgcgcgag gcgaaccggc
      421 tgctcggcct gcgcgacgcg atcggcgagc ttggcaccgt gctggtgctc ggccgccagg
      481 tcgagctgct ctggggtgcg acgccccgcg agctgggcgc gaccccccat gaggcgctgg
      541 agcgctccgg cgcgaccgac ctggtgctga ccacgttcgg gcgctcgatc gagccgcacg
      601 caccctggca cctcggccgg gtcggcaccg cgctgctgcg cggccgggtc aacccggccg
      661 aggtggaggc ggtcctggcg gcggccggga tgggctgggt ccgcttgctg gacaacggcc
      721 gcctgcgcgg cgcctacccg cagctcagcg gcgagggtcc cgcccacgcc taccacctgc
      781 tccccgccgg ggtgagcaag gccgccgcgg ccgcggccta cctggagcgc cggggcctgc
      841 gtgccagcca ggccgccgcg attggcgaca gccccgccga cctgggactg gccggctcgg
      901 tgggcgcggc cttcctggtc cagaacggcg cctgggccgc cgcggcggac accggcgaag
      961 cgggcaacgg cgcggatacc gggacacccg tgatcgtcac gccctccagc gcgggccagg
     1021 gcttcgccga agctgtccac gcgctgctcg atcaccgaaa gcgggcggag cctgggtagc
     1081 ttgggcggcg gatgggtaag ttgggaggaa cccgcggcag gcggcgacgc gaagggcgga
     1141 tccgatggct gagctggtag tggcgtccaa ccggggaccg gtgcagttcg agatcgacga
     1201 cgagggcacc ctgacctgcg gacgtggcgc cggcggcatg gtcgccgcgc tggggccggc
     1261 gctggccggg cagggcggca cctgggccgc cgcggcgatc accgaggggg accgggtcgc
     1321 cgcccggcag gtggcacgca gcggccgggt gcgccaggtg cagctgccgc agggccccat
     1381 ccggctgcgc agcctggtga tcgatccgag gcagtaccag aactactaca accgggtgtc
     1441 caaccggacg ctgtggttcc tgcagcacta cctcttcgac gcgccccgcc atccccgctt
     1501 cgaccaaggc ttccgcacgg cctggcggga ctaccgcgag gtcaaccagg cgttcgccgc
     1561 cgcctgcgac agcgaggcca gccagggcgc gcaggtgttc gtgcaggact accacctggc
     1621 gctgaccccg gcgctgctgc gggctcgccg cccggaactg cacatcgcgc acttcaccca
     1681 ctgcccctgg gccgacccgg gctacttcac gatgctgccc tcaacgaccg cctacgagtt
     1741 cgtcgagggg atgctcggcg ccgacctgat cggcttcctg gtgccgcgct gggcgcggaa
     1801 cttcctgcgc tgctgcgagg agctgggctt cgaggtgaac cgcagggctc agacggtgcg
     1861 cgcccctgac ggccgcgacg cgcgggtgcg cgcctacccg ctcggggtcg atgacggcga
     1921 cctgcgcgcc cgcgtcgccg aacccgacgt ccaggcacag cgccgcagcc tccgcgacct
     1981 ggcgcggggc cgcagcctga tcgtccgggt ggaccgcatg gagctgtcca agaacatcct
     2041 gcggggcctg gacggctaca ccatgctgct ggagcagaac ccccgcctgc gcggccgggt
     2101 ggtccacttc gcgctcgcct acgcctcccg ccgcgacctg cccgagtacc aggcctacac
     2161 cgccgaggtc cgccacgcgg tcgacgcgat caacgagcgc ttccgcaccc ggagctggga
     2221 gccggtgcgg ctggaactgc gcgacaacta cccgcgcgca ctcgccgcca tggccgaggc
     2281 cgacgtgctg ctggtcaacc cggtgtggga cggcatgaac ctggtcgcca aggaaggcat
     2341 ggcggtgagc gaacgcgacg ccgtcctggt cctgtcgcgc aatgccggcg ccgccgacga
     2401 cctggccgag ggcgccctgc tggtcaaccc cttcgacacc gccgagctgg ccgccagcct
     2461 cgccagcgcc gtgacgatgc ccgccgccga ccgcgccaag cgggcggccc tgctgcggac
     2521 ccaggccagc gccctgccgc cccaggactg gttcgccgcc cagcgcaacg acctcgaacg
     2581 catgggtcgg acccgctcct agatgtcctt gtagttcccg ccgatcacca cgtgcagggc
     2641 gatgtcgcgg gacaggctgg agggtgccgg cgcgatcacc ttgaaggcgg ggaagcgcgc
     2701 ctggagggcg agcgcgtcgg cctggtggcc ctcggtgtag tagatccgcg agacggtgaa
     2761 gtgcccgaag gcggtctgcg tggcgaccac gtcgtagccg gcggcacgca gcttgtcggc
     2821 cacctggtgg gccaggccgg ggacgaagac gccgttgagg acctgcacgg tcacgcccgc
     2881 gacccgcggc agggtggtgg tcgagacgct ggccgtcgtc gccggctcgc tgctgggcgg
     2941 gaccatggag gtcgtggtcg ccgccggggc cggggcggac gtcggtgggg ctcccagcag
     3001 cccgccgagc gtcgtggagg tggccgcggg cgcggagctg accgaggccg tggccttggc
     3061 ggcgcggtgc ggctgcgccc ggctcagctg gtcgaccgcg atgaacacgg cgaggaacac
     3121 cgcgaacacc gccgccgcgc gagcggtcgg accgggacgc actcccacct cctagagccg
     3181 tgaacccgac gtagggatcg agcctacgtc aacggcgttg ccgatgccgg gaagctcgct
     3241 gttcccgtag ctggccgacc agggccggct cggcggcgag cgcggccggg tcgtcgatca
     3301 cccggctgag caactggtag tacgcggtcg ggtggaggcc gaactcacgc cggatggcct
     3361 cttccttggc gccggcgtgt ttccaccagc gccgttcgaa cgcgaggacg gaaaggtccc
     3421 gctcggtcgc cattcgctcc ccctcacgac ggttctattg aaaggctccc cgtctaaacc
     3481 gggtaattct gcgcagccac gcatcggtga aatcgtcgat cttcgccata ccggaaggcc
     3541 caacatgtag tggttggcgg gacactaact cccacatctt gtggaaatca aggcttgact
     3601 tgacaggatc gcgaactgtg gaacgctgag cacgtgattt ggcagttcgc tccagagcgg
     3661 gtaaagtcca cgccgtttgg tcacggaatg gtctctcacc ggtaacagcc gaatcctgcg
     3721 tcaccggcgc gggagtgggc accgcaccag aaccatgaca gtgcgaccag cgcggcctct
     3781 ccacccccgg agaggagggc acccccgtcc atcgcccgct ccgcccacag gctcgatcga
     3841 aggaggctgg gcttgccgtt cggaaaaggc cgtcgacacg gactcgcgct gcggctcctg
     3901 cttctcgcag ccgtcgcgct cccgttgacg acactgtcgc tgacaatcca gaacggcgcc
     3961 caggcggccc ccacccggac cgcgcagctc aaggcgcagc tcgacaagct caaccagcgg
     4021 gccgaccagc tggtcgaggc ctacgaccag gccaacgccg gccttgcccg cacccgcggc
     4081 ctgctcggct ccctgaagac ggacgaggcc gacgccggcc agaccctgca ggggctgcag
     4141 cagcgcgtcg gcgagcgcgc ggccgtcgcc tacatggagg ggcccggcaa cgccctcgcc
     4201 gagctgctca acggcagcaa cccggccgac accatcgacc ggatgcagat cctcaacctg
     4261 ctggcgcagc aggacggcga cctcgccgcc gccctccggg tcgcgggcag gaacttcggc
     4321 gcccgcaggg ccgccctggt ggcggcagag aagggccagg ccgccgaggt tgacgcgctc
     4381 gccggcaaga aggccgcggc cgaggcagcc gtggccaaga cccagcggct gctcaacagc
     4441 ctccaggcgg ccgaccgcac ggccctgctg ggcgcggtca acggcgcctc ctcggggagc
     4501 ctcccgccga tcaccgccag tggcgcggtc gcgaaggtgc tgcggtacgc cgaagcccag
     4561 gtcggcaagc cctacgcctt cggcgccgcc gggccgagtg ccttcgactg ctccggcctc
     4621 accatgatgg cctgggccca ggccggcgtc tcactccccc acaccgccgc cggccagtac
     4681 gacgtcatct cccacgtctc cacgagccag atcaggcccg gcgacctgct cttctactac
     4741 tcccccatct cccacgtcgc gatctacgtc ggcaacggga tgcagatcgc cgccacccat
     4801 accggcgact tcgtcaggct ccaggcgatc cactccgggc tggtcggcgc cggccggccc
     4861 tgagcctccc accccaccgc gcctccctcc tcccacgcgc ctcccgaaga acgaaacgaa
     4921 gttcccccat gctgaaggcc ctgtgacgtc acagggcctt cagcatctcc gtgcagcggc
     4981 gtgtgcgagc tgggatcagg ggaacaggcc gcgggtgcgg tgcgcctcgg cgacccggcc
     5041 gacgccgatc acctgggcgg cctggcgcat gctgagcttg cgctcctgcg cgagggcggt
     5101 gacctcctcg taggcgcgct ccatcaccgc acgcatgcgg gtggtgacgt cctcgccgga
     5161 ccagaagtag gcctgcaggt cctggaccca ttcgaagtac gacaccgtca ccccgccggc
     5221 gttggcgagg atgtccggca ccaccaggca gtcgttgtcg tccaggacct tgtcggccga
     5281 cggggtggtg gggccgttgg cgccctccac cacgatgcgg gccttgacgc ggtcgacgtt
     5341 gtcggagcgg atcacg
//
LOCUS       DASWDC010000312         4955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_963909, whole genome shotgun sequence.
ACCESSION   DASWDC010000312 DASWDC010000000
VERSION     DASWDC010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963909"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1119
                     /locus_tag="VF995_10030"
     CDS             232..1119
                     /locus_tag="VF995_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9343938.1"
                     /translation="MSETLRAPTGTIYDRGYRHYDGPREGRSRGVRAIVVASIRRALG
                     LRRGWKTKVVPFGLLVLAFAPAAAFVGIRVVVGEAAGQFIGYGQYLRIVSLLMLLFAA
                     TAGPEILCPDRQTHTLTLILTRPVTRFDYLLAKLVALALVIGLIALLPLLTLFIGNVI
                     TAPHAATYLRHNLGDLGRIMVAGATLTVFFTAVSLAAASLTERRSAAIAGLLGTFLGS
                     AAVTNSIYFASSFSGRRWVLLLSPLNQQSRFVTWVFDLRSGAPSATNFAGATWLAAML
                     VVIAGSLALLAWRIMRVQA"
     gene            1116..2096
                     /locus_tag="VF995_10035"
     CDS             1116..2096
                     /locus_tag="VF995_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9343939.1"
                     /translation="MTAAGTAPASPAAPGAAATSGPAPVAELRHVSKWYGDVVAVADL
                     SFELWPGVTGLLGPNGAGKTTSLKALCGLLRPSQGEVRVFGRTLAGDSALYRSIGVVP
                     DGDRLYPRLTVRSYVRLHADLQGLERPGAAAERAIGEVGMEALAERRLKGLSKGERQR
                     TKVAGAIVHQPSLLVLDEPMTGMDPGQRAHFMDLVRVLGAAGVTVLVSSHILAEVERL
                     ASQVLVLVGGRLAAEGDYHAIRDLMEDQPRTVMVRADEPRRLAPGLIALPTTVAVEVD
                     GEALQARTRSVAAFATALPRLAHDQDVSLREVRTTDESLEQVFRYLVRRR"
     gene            2096..2827
                     /locus_tag="VF995_10040"
     CDS             2096..2827
                     /locus_tag="VF995_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9343940.1"
                     /translation="MTRSLALFRFSFAELLGGRFRRLLVVLLLVVVSLLLPALLNSAS
                     DPSDFMLGLFSNVVLPIALPVVSLVFSTAALGGELRDGTITNLVLKPLSRASILAVEY
                     LSALVATLAVLLPALVAAQLLAAHGLGLAALLKGMLLATVLAAAAYCALGMALSLWAS
                     RALLIGLVYAVVWEGAVVGAAPAASSLSVRGYAEGVLDAILRGSGGPTLDARLGPVSA
                     TLLALIVTVGALALAVRRLARMDFR"
     gene            complement(2769..4121)
                     /locus_tag="VF995_10045"
     CDS             complement(2769..4121)
                     /locus_tag="VF995_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9343941.1"
                     /translation="MTPASPSGHAPGRVSPFRPLRHRAFATIWTGAFVSNIGTWMETV
                     AVGVYVTQTTGKAAWTGTIAALTYAPSVLLGPIGGALADHFDRRRYLATIALLETVLA
                     GWLAVLAASHHLSLTAVAVIVLLEGTAVAFYMPATSAMTPDLVPQEELLGAMSLGAAQ
                     WNLGRVVGPALAGLVITAWSLAAAFWANAVSFGAVLASLALVRVPPLARRDGGLRRVW
                     HTIAEGVAAARRDAGIRTALLLLLATTFLVSPFIGLVPAVAIKVFHRGAAGTSALVTA
                     QGAGAVAAALAAGPLAARIGRGRLLLGALLLVGPLAAAYGLAPRFPLAVVGILLLGCC
                     YLAVLSGTSTVCQLRAPRALRARVASLFMLCVGGGHALGLVIQGWLGDRVGLPAVTAA
                     TGLLLLAVVATTRLLRPGLFAAMGAIREPEAAMAMPLPEVSGSPSGPGAGPPVPEPPR
                     "
     gene            4215..>4955
                     /locus_tag="VF995_10050"
     CDS             4215..>4955
                     /locus_tag="VF995_10050"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX9343942.1"
                     /translation="MTLLKLNEPPTLDRPFLVVHLSGWTDAGMAGEAASAFLRARWNA
                     RSLGEFDPDELIDYRARRPVVQLASGHIEAVTWARIELSVAAEIEGGRSAVLLTGPEP
                     DFRWHAFVERVVEVCELLEIEEVFGLGAFPAPALHTNPVAVVGTSKDPELAARLETVP
                     SIVELAAGIQTVLEDRLHLAGIPATGLWARVPPYLAGGTHPPAALALVRTVGRLAGVE
                     IDTTELEAAAKDHLEQVEAAIRERPQIAE"
BASE COUNT          631 a         1834 c         1807 g          683 t
ORIGIN      
        1 tgatccgcga cctcgccgcc gaacacggct ggccgctgcg gcgcctcatc gcgcgagccc
       61 ccagcctgga ggagaccttc ctgcggctgc aggccaccgc cgccgccacc gccgacggcg
      121 cggccccagt ctcggccgcc gccgaaccgc aacggtctcc tgagggggtg ccgcggtgag
      181 cggaacggtg agcgcgctgc cacggtggac cacgattccg gagcgtgccg aatgagcgag
      241 acactgcggg cacccaccgg gaccatctac gaccggggct accggcacta cgacggaccc
      301 agggagggcc gctccagagg ggtgcgggcg attgtggtcg ccagcatccg ccgcgcgctc
      361 ggcctgcgtc gcggctggaa gaccaaggtg gtgccgttcg ggctgctggt gctcgcgttc
      421 gccccggcgg ccgcgttcgt cggcatccgg gtggtggtcg gcgaggccgc cggccagttc
      481 atcggctacg gccagtacct gcgcatcgtc tcgctgctga tgctgctgtt cgccgccacc
      541 gccgggcccg agatcctctg ccccgaccgg cagacgcata cgctcacgct gatcctgacc
      601 cgcccggtga cccgcttcga ctacctgctc gccaagctcg tggcgctggc gctggtgatc
      661 ggcctgatcg cgctgctgcc gctgctcacg ctgttcatcg gcaacgtgat cacggccccg
      721 cacgccgcca cctacctgcg ccacaacctc ggcgacctcg gcaggatcat ggtcgccggc
      781 gcgacgctga cagtcttctt caccgccgtg agcctggccg ccgcgagcct gaccgagcgg
      841 cgcagcgcgg cgatcgccgg gctgctcggc accttcctcg gctccgccgc ggtgaccaac
      901 agcatctatt tcgcctccag cttcagcggc cgccgctggg tgctgctgct gtcgccgctg
      961 aaccagcagt cccgcttcgt gacctgggtg tttgatttgc ggagcggcgc cccgtcggcc
     1021 accaacttcg ccggggccac ctggctggcc gccatgctgg tggtgatcgc cgggtcgctc
     1081 gccctgctcg cctggcggat catgcgggtg caggcatgac ggcggcaggc accgccccag
     1141 ccagccccgc cgccccgggc gcggcggcga cgtccggtcc agcgcccgtc gccgagctgc
     1201 ggcacgtctc caagtggtac ggggacgtgg tcgccgtcgc cgacctctcg ttcgaactgt
     1261 ggccgggggt gactggcctg ctcgggccga acggcgccgg caagaccacc tcgctgaagg
     1321 cgctctgcgg gctgctgcgg ccctcccagg gagaggtgcg cgtgttcggc aggacgctcg
     1381 ccggcgactc cgccctgtac cggtcgatcg gggtggtccc ggacggggac cgcctctacc
     1441 cgcgcctgac ggtccgctcc tatgttcggc tgcacgcgga cctgcagggc ctggagcgcc
     1501 ccggagccgc cgccgaacgc gccatcggcg aggtcggcat ggaggcgctg gccgagcgcc
     1561 gcctcaaggg cctctccaag ggcgaacgcc agcgcaccaa ggtcgccggc gcgatcgtgc
     1621 accagccctc cctactggtg ctggacgagc ccatgaccgg catggacccg ggccagcgcg
     1681 cccatttcat ggacctggtc cgcgtgctcg gcgccgccgg ggtgaccgtg ctggtctcca
     1741 gccacatcct cgccgaggtg gagcggctcg cctcgcaggt gctggtgctg gttggcggcc
     1801 gcctcgccgc cgagggcgac taccacgcca tccgcgacct gatggaggac cagccgcgca
     1861 ctgtgatggt gcgggccgac gagccgcggc ggctcgcgcc aggcctgatc gccctgccca
     1921 ccaccgtcgc ggtcgaggtg gacggcgagg cgctgcaggc ccgcacccgc tcggtggccg
     1981 ccttcgccac cgccctcccc cgcctcgccc acgatcagga cgtcagcctg cgcgaggtcc
     2041 gcaccactga cgagtccctg gagcaggtct tccgctactt ggtgaggcgg cgctgatgac
     2101 ccgaagcctt gccctcttcc gcttcagctt cgcggagctg ctcggcggcc gcttccggcg
     2161 gctgctggtg gtgctgctgc tggtggtggt gtcgctgctg ctgccggcgt tgctcaactc
     2221 cgcctcggac ccatcggact tcatgctggg gctgttcagc aacgtggtcc tgccgatcgc
     2281 gctgccggtg gtgagcctgg tgttctcgac cgcggcgctc ggcggcgagc tgcgcgacgg
     2341 gaccatcacc aacctggtcc tcaaaccgct gtcgcgggcc tcgatcctgg cggtcgagta
     2401 cctgtccgcg ctggtggcga ccctggcggt gctgctgccg gcgctggtcg ccgcgcagct
     2461 gctcgccgcc cacggcctcg gtctcgccgc cctgctgaag ggcatgctgc tggcgaccgt
     2521 cctggccgcg gccgcctact gcgcgctcgg catggcgctg tcgctttggg cctcccgcgc
     2581 gctgctgatc gggctggtct acgcggtggt ctgggagggt gcggtggtgg gcgcggcgcc
     2641 cgccgcgtcg tcgctgtcgg tgcgcggcta tgccgagggg gtcctggacg ccatcctgcg
     2701 cggcagcggc ggccccaccc tggatgcccg cctgggtccg gtcagcgcga ccctcctggc
     2761 cctgatcgtc accgtggggg ctctggcact ggcggtccgg cgcctggccc ggatggactt
     2821 ccgctgacct ctgggagcgg catcgccatt gccgcctctg gctctcggat ggcgcccatg
     2881 gcggcgaaca ggccggggcg cagcaggcgg gtggtggcga cgacggcgag gagcagcagg
     2941 ccggtggcgg cggtgacggc gggcaggccg acgcggtcgc cgagccagcc ctggatgacc
     3001 aggccgaggg cgtggccgcc gccgacgcag agcatgaaca ggctggcgac ccgggcgcgg
     3061 agggcacgcg gggcgcgcag ctggcagacc gtgctggtcc ctgagagcac tgccaggtag
     3121 caacagccga gcagcaggat cccgacgacc gccaagggga accggggcgc cagcccgtag
     3181 gccgcggcga gcggccccac cagcagcagc gcgccgagca gcaggcgtcc ccggccgatc
     3241 ctggcggcca gcggcccggc ggccagagct gccgcgaccg ccccggcacc ctgcgcggtg
     3301 accagggccg aggtcccggc ggcgccccgg tggaacacct tgatcgcgac cgccgggacc
     3361 aagccgatga acggcgacac caggaaggtc gtcgcgagca gcagcaacag ggcggtacgg
     3421 atgccggcgt cccggcgcgc ggcggcgacc ccttcggcga tggtgtgcca gacccgcctg
     3481 aggccgccgt ccctccgcgc cagcggcggg acccggacca gggcgagcga cgcgagcacc
     3541 gcgccgaagc tcaccgcgtt ggcccagaac gccgcggcca ggctccaggc ggtgatcacc
     3601 agcccggcca gcgccgggcc gaccacccgc ccgaggttcc attgcgccgc gccgaggctc
     3661 atggcgccga gcagttcctc ctgcggcacc aggtccggag tcatggccga ggtcgccggc
     3721 atgtagaacg cgaccgccgt gccctccagc agcacgatca ccgcgaccgc ggtgaggctc
     3781 agatgatggc tggcggccag gaccgcgagc cacccggcga gcacggtctc gagcagggcg
     3841 atcgtggcca ggtagcggcg gcggtcgaag tggtcggcga gcgccccgcc gatcgggccg
     3901 agcagcaccg acggcgcgta ggtcagcgcc gcgatcgtcc cggtccaggc ggccttgccg
     3961 gtcgtctggg tgacgtagac gccgactgcg accgtctcca tccaggtgcc gatgttggag
     4021 acgaacgccc cggtccagat ggtcgcgaac gctcggtgcc gcagcggccg gaacggggag
     4081 acccggccag gcgcatgacc ggaaggggag gcgggagtca cgggaccacg gtagtcccgg
     4141 ccggcaaggc gaattgcccg cagtgcgaca tgtggagatt cccgcgaccc gcctggagcg
     4201 gctaccctgc ccctatgaca ctcctcaagc tcaacgagcc gcccaccctg gatcggccgt
     4261 tcctggtcgt gcacctgtcc gggtggacgg acgcgggcat ggccggcgag gcggcgagcg
     4321 cgttcctgcg tgcccgctgg aacgcgcggt cgctggggga attcgacccg gacgagctga
     4381 tcgactaccg tgcccgccgc ccggtcgtgc agctcgccag cggccacatc gaagcggtga
     4441 cctgggcgcg gatcgagctg tcggtcgccg ccgagatcga ggggggccgc tcggcggtgc
     4501 tgctcaccgg tccggagccg gacttccgct ggcacgcctt cgtcgagcgg gtggtcgagg
     4561 tctgcgagct gctcgagatc gaggaggtgt tcgggctcgg cgccttcccg gcgccggcgc
     4621 tgcacaccaa cccggtcgcg gtggtcggca cctccaagga ccccgaactg gccgcgaggc
     4681 tggagaccgt cccgtcgatc gtggagctgg ccgccggcat ccagaccgtg ctcgaggacc
     4741 gcctccatct ggccggcatc cccgccaccg gcctgtgggc ccgggtgccg ccgtacctgg
     4801 cgggcgggac ccacccaccg gccgcgctgg cgctggtgcg cacggttggg cgcctcgccg
     4861 gggtcgagat cgacaccacc gagctggagg ccgccgccaa ggaccacctg gagcaagtcg
     4921 aggcggccat ccgcgagcgg ccccagatcg ccgag
//
LOCUS       DASWDC010000313        14447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1131648, whole genome shotgun sequence.
ACCESSION   DASWDC010000313 DASWDC010000000
VERSION     DASWDC010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14447
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131648"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VF995_10055"
     CDS             <1..1103
                     /locus_tag="VF995_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX9343943.1"
                     /translation="AGQWIRPDHYGDPAAEVRNVRGGVGIIDVTPLGKLDLRGPDVPK
                     LLNLLYVNKWSKLDVGSVRYGVMCAEDGVVLDDGVTGRLEEGRWLMTTTSSGAATVWE
                     WVENWLQTEHADWQVHVTPVTTAYASINVAGPKSRELLERISEGVDLSPEAFPYMRVR
                     LATIAGVADCFMWRIGFTGELSYEIHVPAAYGLHVWETLMTRGADLGIAPFGVEAQRI
                     MRLEKGHFIVGQDTDGLTKAFSAALDWAVKLDKDDFAGKPELVWQREHADAVSLNGDL
                     RLVGLQPVDGQLVPAEASLIVEGEKQIAGRITSSRMSPTLGRSICLGQVAPHLAAPGT
                     EVTVQLPDGRRAPAQVMEHLAHFDPEGVRLRG"
     gene            1096..1776
                     /locus_tag="VF995_10060"
     CDS             1096..1776
                     /locus_tag="VF995_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEX9343944.1"
                     /translation="MAEATVNAPIARSPIPQPAPTAVVDGWEVHAGHSSAPLRLVDCT
                     PLAKVLVRARPGSAVAASLGVGFGHAVRDGHGTLVVGSGPDEWLLLAAPGQEAEVAAR
                     VRATPSGTSTDTLVSVVEVFSHGRALVRLTGTAASKLLAKVCAVDFADRVTPDGNAFR
                     TAVAKIATDVIRDDLDGQRSYLLHCERSSGQYLYEALLDAGAEFGVERDGFAGSGSAG
                     NGFTGREH"
     gene            1780..3489
                     /locus_tag="VF995_10065"
     CDS             1780..3489
                     /locus_tag="VF995_10065"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX9343945.1"
                     /translation="MSSVTGPVTDLEIARQATPKPLSEIAAHMGLGEHLLEPYGEGVA
                     KVKLEAIGDLADRPKARYVVVSAITPTPLGEGKTTTTVGLGQAFSHLGKRATVAIRQP
                     SMGPTFGIKGGAAGGGYSQVVPMELLNLHLTGDMHAVTAAHNLLAAMIDNHIYQGDEL
                     GIVQHDVTWRRVLDVNDRALRNIVLGLGSRLDGQPRQSGFDITAASEVMAVLALSTSL
                     RDMRQRLARVVIGYNRHGEAITAEQLKAAGAMTVIMREAIKPNLLQTMENTPVLVHAG
                     PFGNIAHGNSSVVADLIGIHGGDYLVTEAGFGADIGAEKFFNIKCRTSGLVPDAAVVV
                     ATIRALKAHSGKHRVVAGKPLPAALLQENPDEVHLGGANLRKQIENIRLHGVTPVVAI
                     NAFPGDHPSEHEAVREIAASLGVRSAVCTHWADGGRGAAELAEAVAEAAEEPSQFRFL
                     YPDEASLREKIETIATRVYGADSVEYDLVASRQLDTYERNGFGNLPVCIAKTHLSISS
                     DPTLKGAPTGWVLPVREVRASVGAGFVYPICGDMRTMPGLGLHPAAERIDIDQHGEIV
                     GLS"
     gene            3510..4151
                     /locus_tag="VF995_10070"
     CDS             3510..4151
                     /locus_tag="VF995_10070"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX9343946.1"
                     /translation="MAWEPQDPSTRSSEGGGAGGGYLDAPLCSFLDQIAARAPAPGGG
                     AAAAMTVAMAAALVAMSARFATSHLDAATDLCTHAEVLRARVAPLAQADAAAYQRVVE
                     AMRLPGKPSAKAAKARNHAIRKAMDGAAEVPLRVAEIGVDVAKLAATLARDGNPNLRG
                     DAATAGQLAAAGARAAATLVAINVGADDPRTLRAGRMADAAGQSAAETLPGGA"
     gene            complement(4227..4892)
                     /locus_tag="VF995_10075"
     CDS             complement(4227..4892)
                     /locus_tag="VF995_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX9343947.1"
                     /translation="MSKQQAPKQPKQQAAKQGKQQTAKQAAKRRPSAASRKEAERRRR
                     QRQRIYAIVIPLVLIGVVVGGVLLIQAQSNSFPYDKVKTFGTLSRNHVTTAVHYKQTP
                     PVGGDHNPVWQNCGYYGQPLHNENAVHSMEHGTVWITYRPDLPTDQVQRLASMAHAQT
                     YVLVSPYPGLPAPVVASAWSTQERFRSANDPKLSEFVAKYRQGPQTPEPGAACTGGTG
                     TPK"
     gene            complement(4889..6004)
                     /locus_tag="VF995_10080"
     CDS             complement(4889..6004)
                     /locus_tag="VF995_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9343948.1"
                     /translation="MATEQARVALGAFNTLVTFGEELKFLRRRARLTQRQLGLAVGYS
                     EAQICRLEQNQRQPDPITLAALFVPALGLERQPQAAARLLELAARARGIQTPATAAEP
                     EAQREVEPEGGTDWLRERLTAALCQAMTFSAGMRVLDLGCDRAASSIFLAREFGVQVW
                     AGDARAEPAANWRRAGDTGLGALVFPLRLEAHALPFPDGFFDAVVSVGAYRRFGTDDL
                     YLGYLTRFLRPEGQLGVVVPGVAAEVEAEPGAAGGFPPPLRSCWDPEYGSFHSPRWWA
                     SHWARSGLVEVQLADLVPGGWHDWLRWERRPALAEALARDAGRTLGFTRVVARRRARR
                     DARRDAGGRMLGGETDFRTEFVRGSATPDPERFEDRR"
     gene            6134..6565
                     /locus_tag="VF995_10085"
     CDS             6134..6565
                     /locus_tag="VF995_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343949.1"
                     /translation="MEIGKLSPAERIIALFGVLLLIDSFIPWFRICISFLTISRCGSH
                     NAWDKGLSLIAVLLAVLMVAQIAATRFASVKLPNLGSLTWGQVHLIAGVAVLVLVLLQ
                     FIVGDSGVHRYFGIYLGILFSLGLAYGALQRSREPEPLATS"
     gene            6778..8097
                     /locus_tag="VF995_10090"
     CDS             6778..8097
                     /locus_tag="VF995_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9343950.1"
                     /translation="MGVAQTSRVAAEHLAEHLGEGAARSERFILRAFTFGRVAVLVQA
                     TIATAAAWHRYPHRVAMGGLLTAAFLESSVLLVLAQRTTPHGRPILIEHPVLIGTDVA
                     FSAVGLVAGVWLLTGSSLCSWDNFMYPYTFIATTLTGVLVGRFRVMAGIATLLATIYI
                     VAMVERLGLHSGLVWNVLENSASYPVFGLIAWALARELRRLGHSLDQASRQAVAREVE
                     VARERERAGHAQQVHGLRLLAVGAALQRERARLRHFRNLHDRVLQTLEQLSRGGLVHE
                     PGVRAHVDREAAWLRQLIEHELPSGPCDLAAALGEVVEAQAAAGLRVALNTAELLPDQ
                     PLSPELIEALAAAAGEALTNVRKHAGVTQAVLRAASEDDAIVVSVLDQGRGFDPARAS
                     GGRGIPQSLIARVGQVGGAVWVDSVPGAGTSVELRAPAGAISRTKGG"
     gene            8099..8752
                     /locus_tag="VF995_10095"
     CDS             8099..8752
                     /locus_tag="VF995_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9343951.1"
                     /translation="MEAPNLTCPIRVAVVDDHPVARWGIEHVFAGRSDIRVVASASSV
                     EELEALGRHLGTDVVMLDLYLGSDVPAIAAIGQLATHAKVLVVSVSGRRADVLAAIEA
                     GADGYLTKQADDAAFAASVRTVAGGGFYLSSQLAGMLRADFRRGDGPRRPRAELSARE
                     AEALRYIAQGFTHAQAATRMNITEATISTYVKRVRQKLKAGNKADLTRRAMELGHLR"
     gene            8883..10379
                     /locus_tag="VF995_10100"
     CDS             8883..10379
                     /locus_tag="VF995_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343952.1"
                     /translation="MDRATRRRGSRPLLVTLALLLALAASGLLTARPAVADPPGGLYP
                     DNVYGPGGACCYGQFGTWFSSGGLGLKGRAVWTWSNGATEDSHAQWDAPNLDENSPYT
                     VDAFIPHNFATAKAHYVVSGMGASQEVVRDQNPVSDNWLNLGQFCPDTRGHLFVRLTD
                     RGDPAGSTQITADAMRFTRVPGGQCAHQRPVLLVRGINVTTDPGGDVKAYWGPLPGFL
                     ANHGWTNLHYIAYYHHDVNGDFIPNYGRTVPPNNGEYNGGSACTPGATSTSPDLNCSL
                     RHIAWNLAWYIYRNWSSKNIDVRIAAHSQGGLITRWMLYRVQNHDPAFPPSLLVSNVV
                     TLGTPHSGAWLAAVLNTNPPILATQINELLPPEDPNGGFTRELNNNAKHPNAANGTDW
                     TLIASEAFATVDSGNGLPLHDGDQVVTAARALGGMDNSTIHRVVYMAGASICHPGPIP
                     GGCVRYVDAVGTSLVPIKESDMGAPWTVPGPGIQVGPWTDNALFFRTW"
     gene            10391..11665
                     /locus_tag="VF995_10105"
     CDS             10391..11665
                     /locus_tag="VF995_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9343953.1"
                     /translation="MRPIRFRRPHRLLLGLGLGAALGVSLLAAPVVAAPATAPVTALA
                     RATLDRAGLNGAVTAAIERRAHITDAAAQAAGRAFQVNLKRRDAAGSWAFGSAVLTAP
                     HRAGAYPLGWLFVAERAAAGWQVALEGDAGFAFLTAPAPSAVMPAREKALLAPVTAAQ
                     RPGARPDASQAAAASNNTGRRLPYAIGQTWTLTGGPHGWSGTDRPFSSIDLSGGDGRV
                     LAAGGGNVYTMCGNNKGWLRIIHPGGYATDYYHLFNNIAPPDGTAISEGAFIGNIGTD
                     TSCGGSASGPHVHFAIRLNGAYVAWNWRTAGKWVLWEGAAAYQGFALHGSTRVNVGGG
                     LFNYGALGANQGIVDANGGGTVNKRSGPGTGFAVVGSVGDGATITIPCWRNGTTHTGR
                     YGTTSVWDKLTDNTWVSDAFVYTGVNTIGPTC"
     gene            complement(11866..12483)
                     /locus_tag="VF995_10110"
     CDS             complement(11866..12483)
                     /locus_tag="VF995_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX9343954.1"
                     /translation="MLDFGGVLTTNFFEPFERFCARHGLAPNTIHHLMSADPVGRALW
                     YDVERGAINQPEFETKLAAMLGLPPDGLVEQLLAELRPEPRMLAAVAAAHGAGVRVAI
                     ISNSWGADPYDPYEDWELARLADVVLISHELGMRKPDPRIYRLAAERLARAPVACVLV
                     DDIASNLPAARELGMRTIHHVDVETTISALEGLLALPLRGRWADP"
     gene            complement(12540..12725)
                     /locus_tag="VF995_10115"
     CDS             complement(12540..12725)
                     /locus_tag="VF995_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343955.1"
                     /translation="MTPTPSSITNATIMADFAVGRCGLTRDDTQLWLADLRRLGAERC
                     YFFSLNRYLFTANKPTR"
     gene            complement(12709..13347)
                     /locus_tag="VF995_10120"
     CDS             complement(12709..13347)
                     /locus_tag="VF995_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9343956.1"
                     /translation="MTAEPLFDSAASAKLDAVYRTPDVVTQRHETLIALRLGEGERVL
                     DVGSGPGLLAAEMAERVGQTGQVAGVDISPSMVRLARQRCARTPIRDRIHFTRADATA
                     LPFADSTFDAAVSTQVYEYVSDLRTAFAELHRVLRHGGRVVIVDTDWDSIVWNAGDQA
                     RMRQVLAAWTERFADPHLPRTLARQLGDAGFVVHGCSALVLLNAGPGYDANT"
     gene            complement(13451..>14447)
                     /locus_tag="VF995_10125"
     CDS             complement(13451..>14447)
                     /locus_tag="VF995_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018789340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9343957.1"
                     /translation="VESIRMLTPAGPSESRRLPASGAGPSPDRLALGSEGTLGVITAA
                     WLRVQPRPARRARATVAFPTFEAGAAAVRSIVQAGLTPANCRLVDAAEAAMTGTLATG
                     EAVLLLGFEAVSPDLPVDAELAVAIELAAQLDGHLTSSEAVDSRVREAAADPRARQGG
                     GAASPGGARSPADASGSWRAAFLRAPYLRESLVLLGVLAETFETAATWDRLDALVAGV
                     RAAVGDALGRVCGGGTVTCRLTHAYPDGAAPYFTVLAPARRGSELAQWAEIKAAAAEA
                     VLASGGTITHHHAVGRDHRPWYDRQRPDPFAHALRAAKAALDPAGILNPGVLVDP"
BASE COUNT         2103 a         5272 c         5032 g         2040 t
ORIGIN      
        1 tcgccgggca gtggatccgc cccgaccact acggcgaccc cgccgccgag gtccgcaacg
       61 tccgcggagg agtcggcatc atcgacgtca ccccgctcgg caagctcgac ctgcgcggcc
      121 cggacgtgcc gaagctactg aacctgctct acgtcaacaa gtggtcgaag ctcgacgtcg
      181 gctcggtccg ctacggggtg atgtgcgccg aggacggcgt ggtgctggac gacggcgtca
      241 ccggccgcct ggaggagggt cgctggctga tgaccaccac ctcctcgggc gccgccacgg
      301 tctgggaatg ggtcgagaac tggcttcaga ccgaacatgc ggactggcag gtgcacgtca
      361 ccccggtcac caccgcctac gccagcatca acgtggccgg cccaaaatcc cgggagctgc
      421 tggagcggat cagcgagggg gtggacctct ccccggaggc gttcccctac atgcgggtgc
      481 ggctggcgac catcgccggg gtggcggact gcttcatgtg gcggatcggc ttcaccggcg
      541 agctgtccta cgagatccac gtgcccgccg cctacggcct gcacgtctgg gagacgctca
      601 tgacacgggg cgcggacctc gggatcgcgc cgttcggggt cgaggcgcag cggatcatgc
      661 gcctggagaa gggtcatttc atcgtggggc aggacaccga cgggctcacc aaggcgttca
      721 gcgccgccct ggactgggcc gtgaagctgg acaaggacga cttcgccggc aagccggagc
      781 tggtctggca gcgcgagcac gccgacgccg tcagcctcaa cggcgaccta cggctggtcg
      841 gcctgcagcc ggtcgacggc cagctggtcc cggcggaggc gagcctgatc gtcgagggtg
      901 agaagcagat cgccgggcgg atcacctcca gccggatgtc gccgacgctg ggccgctcga
      961 tctgcctcgg gcaggtcgcg ccgcacctgg ccgcgcccgg caccgaggtc accgtgcagc
     1021 tgcccgacgg gcgccgcgct cccgcccaag tgatggaaca tctcgcccat ttcgacccgg
     1081 agggggtgcg cctgcgtggc tgaggcaacc gtgaacgccc ccatcgcccg gagccccatc
     1141 ccccagccgg cgccgaccgc ggtcgtggac ggctgggagg tccacgccgg gcactccagc
     1201 gcgccgttgc ggctggtcga ctgcaccccg ctggccaagg tcctggtccg cgcccggccg
     1261 ggcagcgcgg tcgcggccag cctcggcgtc ggcttcggcc acgccgtccg ggacgggcac
     1321 ggtacgctgg tggtgggctc cgggccggac gagtggctgc tgctggccgc ccccggccag
     1381 gaggccgagg tcgccgcgcg ggtccgcgcc actccctccg gcacgagcac ggacaccttg
     1441 gtgagtgtcg ttgaggtgtt cagccacgga cgggcgctgg tccggctgac ggggacggcc
     1501 gcgtcgaagc tgctcgccaa ggtctgcgcg gtcgacttcg cggaccgggt gacgcccgac
     1561 ggcaacgcgt ttcgcaccgc cgttgcgaag atcgcaacag acgtcatccg cgacgacctc
     1621 gatgggcaac gctcgtacct gctgcactgc gagcgctcct cggggcagta cctgtacgag
     1681 gcgctgctcg acgccggggc cgagttcggc gtcgagcgcg acggctttgc cgggagcggc
     1741 tctgccggaa atggcttcac cggaagggag cattgagaga tgtccagcgt taccggtccc
     1801 gtgaccgacc tggagatcgc gcgccaggca acccccaagc ccctgtcgga gatcgccgcg
     1861 catatggggc tcggcgagca cctgctggag ccctacggcg agggcgtcgc caaggtgaag
     1921 ctggaggcga tcggcgacct ggccgaccgg cccaaggccc gctacgtggt ggtctcggcg
     1981 atcaccccga cgccgctcgg cgagggcaag accaccacca cggtcgggct cgggcaggcg
     2041 ttctcgcatc tcggcaagcg cgccacggtg gcgatccggc agccgtcgat gggcccgacc
     2101 ttcggcatca agggcggcgc ggctgggggc ggctacagcc aggtcgtgcc gatggagctg
     2161 ctcaacctgc acctgaccgg cgacatgcac gcggtcaccg ccgcccacaa cctgctggcc
     2221 gcgatgatcg acaaccacat ctaccagggc gacgaactgg gcatcgtgca gcacgacgtg
     2281 acctggcgcc gggtgctgga cgtcaacgac cgggcactgc gcaacatcgt cctcggcctc
     2341 ggctcgcgcc tggacgggca gccgcgccag agcggcttcg acatcaccgc cgcctccgag
     2401 gtgatggcgg tgctggcgct gtccacctcg ctgcgggaca tgcgccagcg gcttgcccgg
     2461 gtggtgatcg gctacaaccg ccacggcgag gcgatcaccg ccgagcagct caaggccgcc
     2521 ggggcgatga cggtgatcat gcgcgaggcg atcaagccga acctgctgca gaccatggag
     2581 aacacgcccg tcctggtgca tgccgggccg ttcggcaata tcgcccatgg caactcctcg
     2641 gtggtcgccg acctgatcgg cattcacggc ggcgactacc tggtcactga ggccgggttc
     2701 ggcgccgaca tcggcgcgga gaagttcttc aacatcaagt gccgcacctc cgggctggtc
     2761 ccggacgcgg cggtggtggt ggcgaccatc cgggcgctca aggcccattc gggcaagcac
     2821 cgcgtcgtcg ccggcaagcc gctgccggcc gccctgctcc aggagaaccc cgacgaggtg
     2881 catctcggcg gcgcgaacct ccgcaagcag atcgagaaca tccgcctgca cggcgtcacc
     2941 ccggtggtgg cgatcaacgc cttcccgggc gaccacccct cagagcacga ggcggtccgc
     3001 gagatcgccg cgtccctggg ggtccgctcg gcggtctgca cccactgggc cgacggcggc
     3061 cgtggtgcgg ccgagctggc cgaggcggtc gcggaggcgg ccgaggagcc gagccagttc
     3121 cgcttcctct accccgacga ggccagcctc cgggagaaga tcgagacgat cgccaccagg
     3181 gtctacggcg ccgacagcgt cgaatatgac ctggtcgcca gccgccagct cgacacctac
     3241 gagcgcaacg gcttcggcaa cctgccggtc tgcattgcca agacgcacct ttcgatctcc
     3301 tccgacccca cactgaaggg cgcgccgacc ggctgggtgc tgccggtgcg ggaggtccgc
     3361 gcctcggttg gggctggctt tgtctacccc atctgtggtg atatgcgtac gatgcctggc
     3421 ctcggtttgc acccggctgc cgagcggatc gacattgacc agcacgggga gatcgtgggg
     3481 ttgtcatgat gatccgatcc catccaccga tggcatggga accgcaggac cccagcacca
     3541 ggtccagcga gggtggcggc gccgggggcg ggtacctcga cgcgccgctg tgcagcttcc
     3601 tcgaccagat cgcggccaga gcgccggcgc ccggcggggg cgcggcggcg gcgatgaccg
     3661 ttgccatggc cgccgcgctg gtggcgatgt ccgcccgctt cgccacctcg cacctcgacg
     3721 ccgccaccga cctctgcacc cacgccgagg tgctgcgcgc ccgggtggcg ccgctcgccc
     3781 aggccgacgc cgccgcctac cagcgggtcg tcgaggcgat gcgccttccg ggaaagccga
     3841 gtgccaaggc cgccaaggcc cgcaaccacg ccatccgcaa agccatggac ggcgccgccg
     3901 aggtcccgct gcgcgtcgcc gagatcggcg tggacgtggc caagctggcc gcgaccctgg
     3961 cccgcgacgg caacccgaac ctgcgcggcg acgccgccac cgccggccag ctcgccgccg
     4021 ccggcgcccg cgccgccgcc accctggtgg ccatcaacgt cggcgccgac gacccgcgca
     4081 ccctgcgcgc cgggcgcatg gccgacgccg ccggccagtc agcagcggag acgctcccgg
     4141 gcggcgcctg acgctgaggg ggcggcgccc gcggcggagc tccgccccct cggcatcaca
     4201 gcttccgtgt cctggctacc cgggcgtcac ttgggagtgc cggtgccgcc ggtgcaggca
     4261 gcgccgggct cgggagtctg ggggccctgg cggtatttgg cgacgaactc gctgagcttg
     4321 gggtcgttgg cggagcggaa gcgttcctgg gtgctccagg ccgaggcgac caccggggct
     4381 ggcaggcccg gatacgggct gaccagcacg taggtctggg cgtgggccat gctggcgaga
     4441 cgctggacct gatcggtggg gaggtctggg cggtaggtga tccagacggt gccgtgctcc
     4501 atcgagtgga ccgcgttctc attgtgcagc ggctggccgt agtagccgca gttctgccag
     4561 acggggttgt ggtcgccgcc gaccggcggg gtctgcttgt agtgcaccgc cgtggtgacg
     4621 tggttgcggc tgagggttcc gaaggtcttc accttgtcat aggggaagct gttgctctgc
     4681 gcctggatca gcaggacgcc gccgaccacg acgccgatca gcaccagcgg gatgacgatc
     4741 gcgtagatcc gctgccgctg gcggcgccgg cgctcggctt ccttccggct ggcggcggac
     4801 gggcgccgct tggccgcctg cttggcggtc tgctgcttgc cctgcttggc cgcctgctgc
     4861 tttggctgct ttggcgcctg ctgcttgctc atctgcgatc ctcgaagcgc tcagggtcgg
     4921 gggtggcgga tccacgcacg aattctgtgc ggaaatcggt ctcgccacca agcatacgtc
     4981 ccccggcgtc gcggcgtgcg tcgcggcgtg cgcggcggcg ggccaccacc cgggtgaacc
     5041 ccagcgtgcg gccggcgtcg cgggcgaggg cctcggccag cgccgggcgg cgctcccagc
     5101 ggagccagtc gtgccagccg ccgggtacca ggtcggcgag ctggacctcg accagaccgg
     5161 accgcgccca gtgcgacgcc caccagcgcg ggctgtggaa ggagccgtac tccgggtccc
     5221 agcacgaccg cagcggcggc gggaagccgc ccgccgcgcc gggctcggcc tcgacttcgg
     5281 cggccacgcc tggcaccacc acgccgagct ggccctccgg ccgcaggaac cgggtgaggt
     5341 agccgaggta gaggtcgtcg gtgccaaaac ggcggtaggc gcccacgctg accaccgcgt
     5401 cgaagaagcc gtccgggaac ggcagcgcgt gcgcctccag ccgcaacggg aagaccaggg
     5461 ccccaaggcc cgtgtcgccg gcccggcgcc agttggccgc cggctcggct cgcgcgtcgc
     5521 cggcccagac ctggacgccg aactccctgg ccagaaagat cgagctggcg gcccggtcgc
     5581 acccgaggtc gagcacccgc atcccggcgg aaaaggtcat cgcctggcag agcgccgcgg
     5641 tcaaccgttc ccgcagccaa tccgtgccgc cttcaggttc aacctctcgt tgagcttctg
     5701 gttccgccgc cgtcgctggc gtttgaatac cgcgcgcccg cgccgccaat tcgagcagac
     5761 gggcggcggc ctgcggctgg cgctccagtc cgagcgcggg tacgaacagc gcggcgaggg
     5821 tgatggggtc gggctggcgc tggttctgct ccagccggca gatctgcgcc tcgctgtagc
     5881 ccacggccaa gccgagctgc cgttgcgtca ggcgggcccg ccgccgcagg aatttcagct
     5941 cctcaccaaa agttacgaga gtattgaagg cgccaagagc gaccctggcc tgctcagtcg
     6001 ccaaccagta ttttcctttc atgtggacgt ttcggtgcgg cgtatgcgga ctctgtcaag
     6061 aaatgtcaac gaaggcaagc ggccgtgcta tgcaatgcgc gagcgacgac acaacaagcc
     6121 aaggggtcga aatatggaaa tcggaaagct ctcccccgcc gaacgaatta tcgcgctgtt
     6181 tggggtgctc ctgctaatcg actcgttcat cccctggttc cggatctgca tctcttttct
     6241 caccatcagc cgctgcggct cgcataacgc ctgggacaag ggcctgagcc ttatcgccgt
     6301 cctgctcgcc gtgctcatgg tcgcccagat cgccgccacg aggttcgcct cggtcaagct
     6361 gccgaacctc ggcagcctca cctggggcca ggtccacctg atcgccggcg tcgcggtgct
     6421 ggtgctggtg ctgctgcagt tcatcgtcgg cgacagcggc gtgcatcgct acttcggcat
     6481 ctacctcggc atcctcttct ccctcggcct cgcctacggc gccctgcagc gcagccgcga
     6541 gcccgagccc ctcgccacct cgtagcgcca ccactccgtt gccggagggc gccgcggcgg
     6601 catctccccg atgcccgcgg cgccctccat cgcatacgcc cgacatgtga agctggccgg
     6661 acctctcgcg tctcctgcga ttgatgacgt atattgcggt atcagcgcgg atggccgtga
     6721 atttcgcgca ctcacggtgg gaacgtggca acggggcgcg ggcgtggagg gccgtcgatg
     6781 ggtgtcgcgc agacctcccg ggttgccgcc gagcatcttg ccgagcatct tggggaaggg
     6841 gcggcgcgca gcgagcggtt catcctgcgg gcgttcacct tcggccgggt cgcggtcctc
     6901 gtccaggcca cgatcgcgac cgcggccgca tggcatcgct acccgcaccg ggtcgcgatg
     6961 ggcgggctgc tcaccgcggc gttccttgag agctcggtcc tgctcgtgct ggcccagcgg
     7021 acgaccccgc acgggcgtcc tatcctgatc gagcatcccg tcctgatcgg caccgacgtc
     7081 gcgttctcgg ccgtcggcct ggtcgccggc gtgtggctgc tgaccggttc gagcctgtgc
     7141 tcctgggaca acttcatgta cccgtacacc ttcatcgcca ccacgctcac cggcgtgctg
     7201 gtcgggcggt tccgggtcat ggctggcatc gccaccctgc tcgccaccat ctacatcgtg
     7261 gcgatggtgg agcggctcgg gctccacagc ggcctggtct ggaacgtcct cgagaactcc
     7321 gccagctacc cggtgttcgg gctgatcgcc tgggccctcg cccgcgagct ccgccgcctc
     7381 ggccacagcc tggaccaggc gagcaggcag gcggtcgcgc gggaggtcga ggtcgcccgc
     7441 gagcgggagc gcgccggcca cgcccagcag gtccacgggc tgcgcctgct cgcggtcggc
     7501 gccgcgctgc agcgggaacg ggcacgcctg cggcacttcc gcaacctcca cgaccgcgtc
     7561 ctgcagacgc tggagcagct cagccgcggc ggcctggtcc acgagccggg tgtgcgggcc
     7621 catgtcgacc gtgaggcggc gtggctgcgc cagctgatcg agcacgagct cccctccggg
     7681 ccgtgtgacc tggccgccgc gctcggcgag gtggtcgagg cccaggccgc cgccggcctg
     7741 cgggtcgcgc tgaacaccgc cgagctgctg cccgaccaac ccctgtcgcc ggagctgatc
     7801 gaggccctcg ccgccgccgc cggggaagcg ctgaccaacg tccgcaagca cgcgggggtg
     7861 acccaggcgg tgctgcgtgc cgccagcgag gatgatgcga tcgtggtcag cgtcctggat
     7921 cagggccgtg gcttcgaccc cgctcgcgcc agcggcggcc gtggcatccc ccagtcgctg
     7981 atcgcacgcg tcggccaggt cggcggtgcc gtctgggtcg acagcgtgcc cggcgccggc
     8041 acctccgtcg agctgcgggc cccggccggc gcaatcagca ggaccaaagg ggggtgacat
     8101 ggaggcgccg aacctcacct gtcccatccg tgtcgcggtc gtcgacgacc accctgtggc
     8161 ccgctggggc atcgagcatg tcttcgccgg ccggagcgac atccgcgtgg tcgcctcggc
     8221 cagctcggtg gaggaactgg aggccctcgg ccgccacctc ggcaccgacg tggtcatgct
     8281 cgacctgtac ctgggctccg acgtccccgc gatcgcggcg atcggccagc tcgccacgca
     8341 cgcgaaggtc ctggtcgtct cggtgtccgg caggcgtgcc gacgtcctcg ccgccatcga
     8401 ggcgggcgcc gacggctacc tcaccaagca ggccgacgac gcggcgttcg cggccagcgt
     8461 ccggaccgtc gccggcggcg gcttctacct ctcctcccag ctcgccggca tgctccgcgc
     8521 cgacttccgt cgcggcgacg gcccccgccg gccccgcgcc gagctgagcg cccgcgaggc
     8581 cgaggcgctg cgctacatcg cccagggctt cacccacgcg caggccgcca cgcgaatgaa
     8641 catcaccgag gcgaccatca gcacctacgt caagcgggtg cgccagaagc tcaaggctgg
     8701 caacaaggcc gacctcaccc gccgcgccat ggagcttggc cacctccggt agcggcccgt
     8761 cacggcccgt caacgtccct gttcggatga cagcgcccgc cccgattcgg ggactgggtg
     8821 ccacctcgcg gcgcgcccaa gatcggtggg ccccgttccc ccctccaccc gcgaggtgcc
     8881 ctatggaccg cgcgacccgg cgccgtggga gccgaccgct cctcgtcacc ctcgccctgc
     8941 tgctggcgct cgccgcctcc ggcctgctca ccgcccgtcc cgccgtggcc gacccgcccg
     9001 gcggcctcta tccggacaac gtctatggcc ccggcggcgc ctgctgctac ggccagttcg
     9061 gcacctggtt ctccagcggc ggcctcggcc tcaagggccg cgccgtctgg acctggtcga
     9121 acggcgccac cgaggactcc cacgcccagt gggacgcccc caacctggac gagaactccc
     9181 cgtacacggt ggacgcgttc atcccgcaca acttcgccac cgccaaggcc cactacgtcg
     9241 tctccggcat gggcgcctcc caagaagtgg tccgcgacca gaacccggtc tccgacaact
     9301 ggctcaacct gggacagttc tgccccgaca cccgcggcca cctgttcgtc aggctgaccg
     9361 accgcggcga ccccgcgggc agcacccaga tcaccgcgga cgccatgcgc ttcacccgcg
     9421 tccccggggg ccagtgcgcc caccagcgcc cagtgctgct ggtccgcggg atcaacgtga
     9481 ccaccgaccc gggtggcgac gtgaaggcgt actggggccc gctccccggc ttcctggcca
     9541 accacggctg gaccaacctg cactacatcg cttactacca ccacgacgtc aacggcgact
     9601 tcatccccaa ctacggcagg accgtgcccc cgaacaacgg cgagtacaac ggcggcagcg
     9661 catgcacccc gggggccacc agcaccagcc cggacctgaa ctgcagcctg cggcacatcg
     9721 cctggaacct cgcctggtac atctaccgga actggtcctc caagaacatc gacgtccgga
     9781 tcgccgcgca cagccagggc gggctgatca cccgctggat gctgtaccgg gtgcagaacc
     9841 acgacccggc gttcccgccg tcgctgctgg tgtcgaacgt ggtcacgctc ggaacgccgc
     9901 acagtggcgc ctggctcgcc gcggtcctca acaccaaccc gccgatcctg gccacgcaga
     9961 tcaacgagct gctgccgccg gaggacccca acggggggtt cacccgcgag ctgaacaaca
    10021 acgccaagca tccgaacgcg gccaacggga ccgactggac gctgatcgcg tccgaggcgt
    10081 tcgccaccgt ggacagcggg aacgggttgc cgctccacga cggcgaccag gtcgtgaccg
    10141 ccgcgagggc actgggcggg atggacaact ccaccatcca ccgggtggtc tacatggccg
    10201 gcgcctcgat ctgccatccc ggcccgatac caggcggctg cgtcaggtat gtggacgccg
    10261 tcggtaccag cctcgtcccc atcaaggagt cggacatggg agcgccatgg accgtgcctg
    10321 ggcctggcat ccaggtcggc ccctggactg acaacgccct gttcttccgc acctggtgag
    10381 gaggcgtccc atgcgaccca tccgtttccg ccgtccacac cgcctcctcc tcgggctcgg
    10441 gctcggcgcg gccctggggg tcagcctgct cgccgccccg gtcgtcgccg cacccgccac
    10501 cgcacccgtt accgcgctcg cgcgagcgac cctcgaccgt gccgggctca acggcgccgt
    10561 gaccgcggcg atcgagcggc gcgcccacat caccgacgcg gccgcccagg ccgccggccg
    10621 ggccttccag gtcaacctca agcgccggga cgccgcgggc agctgggcgt tcggcagcgc
    10681 ggtgctgacc gccccgcacc gggctggcgc ctaccccctc gggtggctgt tcgtcgccga
    10741 gcgcgccgcg gccggttggc aggtcgcgct ggagggcgac gccggcttcg cgttcctgac
    10801 cgcccccgcg ccatcggcgg tgatgccagc ccgggagaag gccctgctcg caccagtcac
    10861 cgccgcccag cgcccgggag cccggcccga cgccagccag gccgcggcgg ccagcaacaa
    10921 caccggccgg cgcctgccgt acgcgatcgg gcagacctgg acgctcaccg gcgggccgca
    10981 cggctggtcg gggaccgacc ggccgttcag ctcgatcgac ctgtccggcg gcgacggccg
    11041 ggtgctggcc gccggcggcg gcaacgtcta cacgatgtgc ggcaacaaca agggctggct
    11101 gcggatcatc caccccggcg gctacgccac cgactactac cacctgttca acaacatcgc
    11161 gccgccggac ggcaccgcga tcagcgaggg cgccttcatc ggcaacatcg gcaccgacac
    11221 ctcctgcggc ggctccgcgt caggccccca cgtgcacttc gcgatccgcc tcaacggcgc
    11281 ctacgtcgcc tggaactggc gcaccgccgg caagtgggtg ctctgggagg gcgcggccgc
    11341 ctatcagggc ttcgcgctgc acggctcgac ccgggtcaac gtcggcggcg gcctgttcaa
    11401 ctacggcgcg ctcggcgcca accagggcat cgtcgacgcc aacggcggcg gcacggtcaa
    11461 caagcgctcc gggccgggga ccggcttcgc tgtcgtcggc tcggtcggcg acggcgcgac
    11521 gatcacgatc ccctgctggc gcaacggcac cacccacacc ggccgctacg gcaccaccag
    11581 cgtctgggac aagctcaccg acaacacctg ggtcagcgac gccttcgtct acaccggcgt
    11641 caacaccatc ggccccacct gctgagcgcg gccgaccagt tcgccgtgga ggacgtcgac
    11701 aacggcctgg agcggttcct caccaccacc aggcggcaga acttcctggt cccaccgcgc
    11761 cgccaccggg ccttcccgct gctcgaactg acgtagcagg cccccgaccg ggtgcggcgc
    11821 cggcctccac cggcgccgca cccacctccc aggcgccgag cttggctagg gatcggccca
    11881 cctgcctcgc agagggagtg cgagcagccc ttcaagcgcg ctgatggtgg tctcgacatc
    11941 gacgtgatgg atggtgcgca tgccaagctc gcgggcggcg gggaggttcg aggcgatgtc
    12001 atcgacgagc acgcatgcca ccggggcacg cgcaagccgc tcggcagcca accggtagat
    12061 gcgcgggtcc ggtttacgca tgccaagctc gtgcgagatc agcaccacat ccgccagcct
    12121 cgccagctcc caatcttcgt aggggtcgta ggggtcggca ccccaggagt tggagatgat
    12181 ggcgacccgc acgcccgccc cgtgcgcggc ggccacggcc gcgagcatgc gcggttcagg
    12241 ccgcagctcg gccagcagtt gctcgacgag gccatcggga gggaggccca gcatcgccgc
    12301 cagtttggtc tcgaactccg gctggttgat cgcgccccgc tccacgtcgt accacagcgc
    12361 cctcccgacg ggatcggcgc tcatcaggtg gtgaatcgtg ttgggtgcca gaccatgacg
    12421 cgcacagaac cgctcgaacg gctcgaagaa attcgtggtc agcacaccgc cgaagtcgag
    12481 caacaatcct tgggctggtg gcgacgccgc ccagcagtgc tcccggcttg gccgctgcat
    12541 caccgggtcg gcttgttggc ggtgaaaagg tagcggttca ggctgaagaa gtagcagcgc
    12601 tccgccccga gccggcgcag atcggcaagc cacagctggg tgtcgtcgcg ggtgagacca
    12661 catcgcccga ccgcgaagtc cgccatgatg gtcgcgttgg tgatgctgct aggtgttggc
    12721 gtcatagcct gggccggcgt tgagcaggac caaggcgctg cagccgtgga ccacgaaccc
    12781 agcgtcgccg agttggcggg caagggtgcg gggaaggtgc gggtcggcga accgttcggt
    12841 ccaggcggcg aggacttggc gcattcgggc ctggtcgcct gcgttccaga cgattgagtc
    12901 ccaatcggtg tccacgatca ccacccggcc cccgtgccgc aggacccgat gcagttccgc
    12961 gaacgcggtg cgaaggtcgc ttacgtattc gtagacctgg gtggagaccg ccgcgtcgaa
    13021 ggtgctgtcc gcaaacggca gggcagtggc gtccgccctg gtgaagtgga tccgatcacg
    13081 gatcggggta cgggcgcagc gctgccgggc gagccggacc atgctggggc tgatgtccac
    13141 cccggccacc tgcccggtct ggccgacccg ttcagccatc tcggcggcga gcagcccagg
    13201 cccggaacca acgtcgagga cgcgttcgcc ctccccgagc cgcagcgcga tcagggtctc
    13261 gtgccgctga gtgacgacat caggagtccg gtagacggcg tcgagcttgg ctgatgcggc
    13321 gctgtcgaac agtggttctg cagtcatgcc acgccctcat catcgttgtc atctgcatgg
    13381 aaccgatgct tttcgtcccg tgatggacgg ctcagcgttc gactgggtcg gctctcggat
    13441 acaaccgagg tcacgggtcc accaagactc cggggttgag gatgccggcg gggtcgaggg
    13501 cggccttggc ggcgcgcagg gcgtgggcga acgggtcggg gcgctggcgg tcataccagg
    13561 ggcggtggtc gcggccgacg gcgtggtggt gggtgatggt gccgccgctg gcgaggaccg
    13621 cctcggcggc ggcggctttg atctccgccc actgggccag ctcactgcca cggcgggcgg
    13681 gggccaggac ggtgaagtag ggcgcggcgc cgtcggggta ggcgtgggtg agccggcagg
    13741 tgaccgtgcc gccgccgcag acccggccga gggcgtcgcc gaccgccgcg cggaccccgg
    13801 cgaccagtgc gtcgagccgg tcccaggtcg ccgcggtctc gaacgtctcc gccagcacgc
    13861 cgagcagcac cagggattcg cgcaggtagg gggcgcgcag gaaggccgcc cgccaggaac
    13921 ccgatgcgtc cgccgggctc cgcgctcccc cagggctcgc cgcacctcct ccctggcgag
    13981 ctctgggatc cgcagcggct tcccgcacac ggctgtccac agcctctgaa ctggtcagat
    14041 ggccgtcgag ctgggcggcc agctcgatcg ccacggcgag ctcggcgtcg actggcaggt
    14101 ccggcgagac ggcctcgaag ccgagcagca gcacggcttc cccggtcgcc agggtgccgg
    14161 tcatggcggc ttcggccgcg tcgaccaggc ggcagttcgc cggggtgagg cccgcctgga
    14221 cgatcgagcg gaccgcggcg gcgcccgcct cgaaggtggg gaaggcgacg gtggcgcggg
    14281 cacggcgagc cgggcggggc tggacccgca gccaggcggc ggtgatcacg ccgagggtgc
    14341 cctcgctgcc gagggcgagg cggtcggggc tcgggccggc gccggaggcg ggcaggcggc
    14401 gggactcgct cgggccggcg ggggtgagca tgcggatcga ctcgacc
//
LOCUS       DASWDC010000314         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_810170, whole genome shotgun sequence.
ACCESSION   DASWDC010000314 DASWDC010000000
VERSION     DASWDC010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810170"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..126
                     /locus_tag="VF995_10130"
                     /pseudo
     CDS             1..126
                     /locus_tag="VF995_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
     gene            complement(398..>2079)
                     /locus_tag="VF995_10135"
     CDS             complement(398..>2079)
                     /locus_tag="VF995_10135"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9343958.1"
                     /translation="QDLKKGETIGIVAAAVILVLVFGALAAAVLPIVLAVMSIVVALG
                     IVSLLGMAFHFSFFVTNMITMMGLAVGIDYSLFIVSRYREERARGRERLDAVAAAGGT
                     ANRAVFFSGLTVVLALAGMLLIPTTIFRSLATGAIAVVLVAVLASLTLLPALLALMGD
                     RINALRVPVLFKRRMENPDRTSGFWAGVASRVMARPVLSLVLAAGVLVAVALPVFGIK
                     TGFSGVSTFPDDVQSKQAFTILARDFSGGLSQPAQIVVDGDVRSAPVSAAIARLRGAL
                     RADPAFGPSALQTNSAGDLALISVPVTGDPNGPAAADAIRQLRSTEIPRAFAGSGTRV
                     LVGGQTAQSVDFFQLTDHYTPIVLAFVLGLSFLLLAVVFRSLVVPLLGVLLNLLSAGA
                     AYGLLVLVNQRGTGAGILGFQQVPTIESWLPLFLFSVLFGLSMDYQVFLLSRIRERYD
                     QTGDNPAAVSFGLRTTGGIITGAALIMVAVFGGFATGRLVMLQEMGFGLAVAVLIDAT
                     LVRSVLVPAALRLLGDRNWYLPSWLHWLPQLRVEVPATHPTRPEPELEPVA"
BASE COUNT          372 a          678 c          779 g          250 t
ORIGIN      
        1 gagcacaccc tcaacatcga cttcctcaac cccacccagg gaccgtccgc ccgggtcgcc
       61 gtcgaactgg acgaagcatc agcgcgagcg ctcgccagcg cgatcctcgc cgcgctcgcc
      121 agcgcccgcc ccgtcgcgaa tccccaacag gaactcaagc aactatttgc ggatgtcccc
      181 tattcgctgg cggacagcct cattgaggat cgccgggccg aggtcgagcg agagcgggac
      241 acctcgccct gaggccttcg acgccaattc gatatggtgc tcatcgccat ctcaccccta
      301 ccgaatgtcg gacatctgcc ggacagcaga gcgcccgtgc cctccattcg gccaacctgg
      361 atgggcacgg gcgtgcgctg tggttgagat cgggaggcta tgcgaccggc tccagttccg
      421 gctccgggcg ggtgggatgg gtggcgggga cttcgacgcg gagctgcggg agccagtgga
      481 gccagctcgg caggtaccag ttgcggtcgc cgaggaggcg gagggcggcg gggacgagga
      541 cggagcggac cagggtggcg tcgatgagga cggcgacggc gaggccgaag cccatttcct
      601 ggagcatgac gaggcggccg gtggcgaagc cgccgaagac ggcgaccatg atgagggcgg
      661 cgccggtgat gatgccgccg gtggtgcgca ggccgaagga gacggcggcg gggttgtcgc
      721 cggtctggtc gtagcgttcg cggatgcggc tgagcaggaa gacctggtag tccatggaga
      781 ggccgaacag gaccgagaac aggaacagcg gcagccacga ctcgatcgtg gggacctgct
      841 ggaagccgag gatccccgcg ccggtgccgc gctggttgac cagcaccagc aggccgtagg
      901 cggcacccgc cgacagcagg ttcagcagca ccccgagcag cggcaccacc agcgagcgga
      961 acaccaccgc cagcagcagg aacgacaggc cgagcacgaa cgcgagcacg atcggggtgt
     1021 agtggtcggt cagctggaag aagtccaccg actgcgcggt ctgcccgccg accagcactc
     1081 gggtgccgct ccccgcgaac gcccgcggga tctcggtgga gcgcagctgg cggatggcgt
     1141 cggcggcggc cgggccgttg gggtcgccgg tgaccggcac tgagatcagg gcgaggtcgc
     1201 cggcgctgtt cgtctggagg gcgctcggcc cgaacgccgg gtcggcccgc agagcccccc
     1261 ggagccgcgc gatcgcggcg ctgaccgggg cggagcggac gtcgccgtcg accacgatct
     1321 gggccggctg gctgagaccg ccggagaagt cccgcgcgag gatcgtgaag gcctgcttgg
     1381 actggacgtc gtcggggaag gtgctgacgc cggagaaccc ggtcttgatg ccgaacaccg
     1441 gcagcgcgac ggcgaccagc accccggcgg cgaggaccag gctcagcacc gggcgcgcca
     1501 tgacgcggct ggcgaccccg gcccagaagc cgctggtgcg gtcgggattc tccatgcgcc
     1561 tcttgaagag cactgggacg cgcagggcgt tgatccggtc ccccatcagc gccagcaggg
     1621 cgggcagtag ggtgagcgag gcgagcaccg ccaccagcac caccgcgatc gcgccggtgg
     1681 cgaggctgcg gaagatggtg gtcgggatca gcagcatgcc ggcgagggcg aggaccacgg
     1741 tcaggccgga gaagaacacc gcgcggttgg cggtcccacc ggcggcggcg acggcgtcga
     1801 ggcgttcgcg gccacgggcg cgctcctcgc ggtagcggga gacgatgaac agcgagtagt
     1861 cgatgccgac ggcaagcccc atcatcgtga tcatgttggt cacgaagaac gagaagtgga
     1921 acgccatgcc gagcagcgaa acgatcccga gggcgaccac gatcgacatg acggccagca
     1981 cgatcggcag caccgcggcg gccagcgcgc cgaacaccag caccaggatg acggcggcgg
     2041 cgacgatgcc gatcgtctcg cccttcttga ggtcctgct
//
LOCUS       DASWDC010000315         7268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_633907, whole genome shotgun sequence.
ACCESSION   DASWDC010000315 DASWDC010000000
VERSION     DASWDC010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633907"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VF995_10140"
     CDS             complement(<1..359)
                     /locus_tag="VF995_10140"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX9343959.1"
                     /translation="MTTQPEPALAVFALPGLPEVQPGDDLAALLLQGVGAAGLELADG
                     DVLAVTSKVVAKAEGRIVPLPADPGERERVRRETIAAETVRVVARRGDLVIAETRHGL
                     VGANALVDNSNAGGDALL"
     gene            complement(356..1342)
                     /gene="cofD"
                     /locus_tag="VF995_10145"
     CDS             complement(356..1342)
                     /gene="cofD"
                     /locus_tag="VF995_10145"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX9343960.1"
                     /translation="MLVALAGGVGAARFLRGLIHVHPAADVVVVGNTGDDLRLHGLAI
                     SPDLDSVAYTLAGVGDEERGWGLAGETWNVRDALGRLGEPGWFALGDRDIATHLLRTR
                     LLAEGAPLSAATAEVCRRLGVPVKLLPMSDQRVETRVEVQGPSGIEDLGFQEYWVGRQ
                     ARDPVRRVTFAGVERARPAPGVLDAIAGAQGIILCPSNPVVSIAPILAVPGIRAAIQA
                     AECRTVAISPIVGGAPVRGMADKLLPAWGIEVSARGVASLYAGLADAFVLDQVDAAEA
                     ADVAALELEAVVAPTLMHTVEDAAALAKVALAALAGPNGNGVGGSGGSASGR"
     gene            complement(1406..2218)
                     /locus_tag="VF995_10150"
     CDS             complement(1406..2218)
                     /locus_tag="VF995_10150"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEX9343961.1"
                     /translation="MPRHEDLDDRLDALLDGHEAVVSHEMAPLLDAAAALRASVAPLR
                     LDPATARRHLREALAGPGASPSRSHARAAAPLGPDPALGPALDTKTSRVSRTRHSRSR
                     PASRRSSRRPLRRLATLALAAALVLVPLVLFAGGALPGNPLYQVKLATEQIHLTTVAW
                     SPPRVADLRTQLAGTRLDELEGLVASGQLDQVPAAIGALDGAVNAAEQSTDDAGVHDP
                     RTRALLDQRLSSLQNQRVAQLSALLKRLPAATPTAARVRIQDAVRRSLDQRR"
     gene            complement(2220..2840)
                     /locus_tag="VF995_10155"
     CDS             complement(2220..2840)
                     /locus_tag="VF995_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9343962.1"
                     /translation="MVAARRREPAAIGRIYAAYAPALFRFFAAELGDRHMAEDLTGSV
                     FTAAIEGLPRFRGPVEALGGWLFRIARHDLYDFRRQAARARLDRLDDVLDEAAAAAGA
                     ADPEEVALDRLEGSRVMAALESLSPDQREVLLLRMAAGLTAPEVAAATGKTTGAVKAL
                     QHRGLASLARVLGLRPTSETSGPGQLDSRRPYPSASPARLGDHEEE"
     gene            3163..3417
                     /locus_tag="VF995_10160"
     CDS             3163..3417
                     /locus_tag="VF995_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX9343963.1"
                     /translation="MSPAVFLPFSPETKPWYEHAACLGKDADCFFPEKGGSTRAAKRI
                     CQTCVVQTECLEYALDNDERFGIWGGLSERERRRLKRRAS"
     gene            3644..6769
                     /locus_tag="VF995_10165"
     CDS             3644..6769
                     /locus_tag="VF995_10165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9343964.1"
                     /translation="MVVSHNGRPFLRETFRGLAAQSRAIDDILVVDTGSTDGSADWAR
                     SRLGEDAVVAVRGQFGRAVMAALRDSRTAGTDWLWLLHDDVAPEPEALERLLAEAESR
                     PSASILGPKLVSWTNPDRLQEIGWSVDRTGRAVSPIEDDEIDQGQHDQIRDVFYVSTA
                     GMLVRRSTLVGVGGFDERMTAFRDDLDLCWRTHLTGGRVLVVPSARVRHFGAASSRTR
                     RTRAVNHPRYLAERHTLAALLKATALRRLPLVLAMALVGGGLRALALALTGRPADALQ
                     ILGAWGWNIKELPVTVVQRRRLQHRRKIPDTALAPLRAAGGQPLRAMLRGLLDLVYGE
                     VGEHVAQVSSNPDEQDLQQQGNTLGKIVASHPVAFTVIGFAVLLALSLRGLLFAPGIA
                     GGALGLFPDSGQTLLREFLAAFHQNGLGSTAAAPPNFAVLGALATLTFNKALLAEKLL
                     LWLALPVAAATCTRALRVLVDSRPARALAGLLYASSPLAFGAIAQGRLGELLFLVLAP
                     PTLAQLPLVFRPDQPRESWRPALRFMVLAAASIVLYPPAFVVLALVVAVAVVAALARP
                     ARGLGRFAVKQAVFLAAAMGGTLVLLAPWSSELLAGLLRGDRPAAAPNLLDLLQLRPG
                     GPGVPGHLVGPVYPALALAAIPLVLPTRRLQAFWLVAGFVGAAVVAAWEAHGLAPLFT
                     DWPGGVLVVGAVAWAAAAGMALGRLRELWEQASSLDPRRVGAGALALLTLLTGLLVTV
                     QFVRGSWAPLQAVGRPSLPATVRQSSARVLWLAGRPDHGVNFAVTGSDGRTLLDDGQV
                     ASGNAAGALRSVVTDVVEARTHAAGRLLQLFGIGYVVVRHGPEEERLTGLVSRQQDLR
                     ARPTEQAELFQGPTVSASIPSVAPAGWLITSDTLPGSVQGMLTGPLIVGLQAAQPLLS
                     GPDAKLPAHVISDLPALPGGGPATIVLPVPADSAWQATVGGTRLRAGVAFGWAQAFRL
                     PEGASGTLDLRRTGQTSREALLLAEGLLVLVALAAMARPTRVAPPVAPSTVDETTGEF
                     RLNTRIREGAR"
     gene            6795..>7268
                     /locus_tag="VF995_10170"
     CDS             6795..>7268
                     /locus_tag="VF995_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343965.1"
                     /translation="MSDDDDRMEYDDFVWDPSEGGRPPGQEPDPAPWRQPFSWETPQQ
                     PEPERDPFRDTGPLDPYPPIGGPAQSGGPAPGNRDRQRFWRSDPEPPLPPDDGGSLNG
                     HTNGHTNGHNGHGGFDDSDAGGRYADFTEALIERLADGVADPHTDPRLPRQPPPPQ"
BASE COUNT          909 a         2687 c         2698 g          974 t
ORIGIN      
        1 agcagcgcgt cgccgccggc gttggagttg tcgaccagcg cgttggcgcc gaccaggccg
       61 tggcgggtct cggcgatcac caggtcgccg cggcgggcga ccacccggac ggtctcggcg
      121 gcgatcgtct cgcggcggac ccgctcccgc tcacccgggt cggccggcag cggcacgatc
      181 cggccctcgg ccttggcgac caccttggag gtgaccgcca gcacgtcccc gtcggcgagc
      241 tccagcccgg ccgcgccgac cccctggagc agcagcgcgg cgaggtcgtc gcctggctgg
      301 acctccggca gccccggcag cgcgaacacg gcgagcgccg gttccggctg ggtggtcacc
      361 ggccgctcgc gctcccgccg ctcccgccga cgccgttccc gttgggaccg gcgagggccg
      421 cgagggcgac cttggcgagc gcggcggcgt cctcgacggt gtgcatcaag gtcggcgcca
      481 ccaccgcctc cagctccagc gcggccacgt cggcggcctc ggcggcgtcg acctggtcga
      541 gcacgaacgc gtcggccagg cccgcgtaga ggctggccac cccccgcgcg gacacctcga
      601 tgccccaggc gggcagcagc ttgtcggcca tgccccgcac cggcgcgccg ccgacgatcg
      661 ggctgatcgc caccgtgcgg cactcggccg cctggatcgc cgcgcggatg cccggcaccg
      721 ccaggatcgg cgcgatcgac accaccggat tggacgggca caggatgatc ccctgcgcgc
      781 ctgcgatcgc gtcgagcacc cccggcgccg gccgggcccg ctcgaccccg gcgaaggtga
      841 cgcgccgcac cgggtcgcgt gcctggcggc cgacccagta ctcctggaag ccgagatcct
      901 cgatccccga tgggccctgg acctcgacgc gggtctcgac ccgctggtcg ctcatgggca
      961 ggagcttcac cggcaccccg agccggcggc agacctccgc ggtcgcggcc gacagcggcg
     1021 cgccctcggc cagcaggcgg gtgcgcagca ggtgggtggc gatgtcgcgg tccccgagcg
     1081 cgaaccatcc cggctcaccg agccgcccca aggcgtcgcg gacgttccag gtctccccgg
     1141 cgagccccca gccgcgctcc tcgtcgccga ccccggccag ggtgtaggcg accgagtcca
     1201 ggtccggtga gatcgccagg ccgtggaggc gcaggtcgtc gccggtgttg ccgacgacca
     1261 cgacgtcggc ggccgggtgg acgtggatca gcccgcgcag aaaacgggcc gcgccgacac
     1321 cgccggcgag cgcgacgagc atgtgcgacc tcaatcgaac gtgacgagac aggagcggtt
     1381 ccccagggag gatacccggc gctccctatc gccgctggtc gagggagcgc cggacggcgt
     1441 cctggatgcg gacccgcgca gccgtcgggg tggcggcggg gaggcgcttg agcagggcgc
     1501 tgagctgggc gacccgctgg ttctggaggg agctgaggcg ctggtcgagc agggcgcggg
     1561 tcctcgggtc gtggacgccg gcgtcgtcgg tggactgctc ggccgcgttc accgcgccgt
     1621 cgagcgcgcc gatcgccgcc gggacctggt cgagctggcc ggaggcgacc agcccctcga
     1681 gttcgtcgag gcgggtcccg gcgagttggg ttcgcaggtc ggccacgcgc ggcggcgacc
     1741 acgccaccgt ggtcagatgg atctgctcgg ttgcgagctt cacctggtag agggggttgc
     1801 cggggagggc gcccccggcg aacagcacca gcgggaccag tacgagcgcc gctgccagcg
     1861 ccagggtggc gagccggcgc agcgggcgcc gcgacgagcg ccgcgatgcg ggccgcgacc
     1921 gggagtgccg cgtgcgcgac acccgcgagg tcttcgtgtc gagggccggg ccgagcgccg
     1981 ggtccgggcc caggggcgcc gcggcccgtg cgtgggatcg ggaaggagat gccccgggcc
     2041 cggcgagcgc ctcgcgcagg tgccgccttg ctgtcgctgg atccagccgc aggggcgcca
     2101 cgctggcgcg cagggcggcc gctgcgtcaa gcagcggcgc catctcgtga ctgacgacgg
     2161 cttcgtgccc gtcgagcagg gcgtccagtc ggtcgtccaa gtcctcatgc cggggcatct
     2221 cactcctcct cgtgatctcc aaggcgtgcg ggagaggcgg atggatacgg cctgcggctg
     2281 tcaagttgcc cggggccgga ggtttccgag gttggccgca ggcccagcac ccgggcgagg
     2341 ctcgccagcc cccggtgctg cagcgccttc accgcgccgg tggtcttccc ggtggccgcc
     2401 gcgacctcgg gggcggtcaa gccggcggcc atgcgcagca gcagcacctc ccgctggtcg
     2461 ggggagagcg actccagcgc ggccatcacc cggctcccct ccagccggtc gagcgccacc
     2521 tcctccgggt cggccgcgcc ggccgccgcc gctgcctcgt cgagcacgtc gtcgagccgg
     2581 tcgagccgcg cacgcgccgc ctggcgccgg aagtcgtaca ggtcgtggcg ggcgatgcgg
     2641 aacagccagc cgcccagtgc ctcgaccggt ccgcggaatc gcgggagccc ctcgatcgcg
     2701 gcggtgaaaa ccgacccggt gaggtcctcc gccatatgcc gatcgcccaa ttcagcggca
     2761 aagaaccgga acaacgccgg tgcgtaagcc gcatagattc gcccgatcgc cgccggctcc
     2821 cgccgcctgg cggccaccac gtccgcctcc tccaatggcg cccgtcccag cggtttctgc
     2881 ccccccgacc cgaacaagca atccccccag agcggtcaca ccacagcaag accgcggaag
     2941 gctaaccgat ttgttcgcct gtgagccagg cggcgcgggt gcggattccg ccgcgactgc
     3001 cgtgactgcc gtaactgccg cagctgcgaa ataaattgcc actcttgcca gcggccgcga
     3061 gttcaaagag aatgtcattg atgtaattac caacgtgaag ttttgggaat agtgcaagca
     3121 aaccgccgac atgggcatcg gtgcggaacc aaggaggcag cgatgagccc agcagtcttt
     3181 cttccgtttt caccagaaac caagccgtgg tacgagcacg ccgcctgtct gggcaaggac
     3241 gccgactgct tcttcccgga aaagggcggt tccacccggg ccgcgaagcg gatttgccag
     3301 acgtgtgtgg tccagaccga atgcctggag tatgccctgg acaacgacga gcgcttcggc
     3361 atctggggcg gcctgagcga gcgcgaacgc cgccgtctga agcgtcgcgc cagctgacca
     3421 agctgtccag ccgcacgccg gctgcgttcg tccgggttcg cgcgcctttc gggggcgttt
     3481 cgagtgaccc ggtttgagat ggctcgggga ctccgtgtta gcatcgcgtg gcccgtgggg
     3541 aggtaggctg ctcctgcggg cgttttcgct gagctgtggc ggcggtaccc ccagaccgct
     3601 tgagaggaag gtcgtggcga cgagtcaagc ccgtgtcatc gccgtggtcg tgtcccacaa
     3661 tgggcggccg tttctgcggg agacgttccg aggtctcgct gcgcagagcc gcgccatcga
     3721 cgacatcctg gtggttgaca cgggctcgac cgacgggagc gccgactggg cccgctcccg
     3781 cctcggcgag gacgctgtgg tggccgtgcg gggtcagttc ggccgcgcgg tgatggcggc
     3841 gctgcgggac tcgcgcaccg ccgggacgga ctggctgtgg ctgctccacg acgacgtcgc
     3901 gcccgagccg gaggcgctgg agcggctgct cgctgaggcg gagtcgcggc ccagcgcctc
     3961 gattctcggc cccaagctgg tgtcgtggac caaccccgac cgcctgcagg agatcggctg
     4021 gtcggtggac cgcaccggcc gggcggtctc gccgatcgag gacgacgaga tcgaccaggg
     4081 ccagcacgac cagatccgcg acgtcttcta cgtctccacc gccgggatgc tggtgcggcg
     4141 cagcaccctg gtcggggtcg gcggcttcga cgagcgtatg accgccttcc gcgacgacct
     4201 ggacctgtgc tggcgcaccc acctgaccgg cgggcgggtg ctggtggtgc ccagcgcgcg
     4261 ggtgcgccac ttcggcgccg cctcctcgcg cacccgccgc acccgcgcgg tcaaccaccc
     4321 ccgctacctg gccgagcggc acaccctggc ggccctgctg aaggcgaccg cgctgcggcg
     4381 gctgccgctg gtgctggcga tggcgctggt tggcgggggg ttgcgcgcgc tggcgctggc
     4441 gctgaccggc cgccccgccg acgccctcca gatcctcggg gcgtggggct ggaacatcaa
     4501 agaactgccg gtcaccgtcg tgcagcgccg ccggctgcag caccgccgca agatccccga
     4561 cacggcgctg gcgccgctgc gggcggccgg cgggcagccg ttgcgggcga tgctgcgcgg
     4621 cctgctcgac ctggtctacg gcgaggtcgg cgagcacgtc gcgcaggtgt cgtccaaccc
     4681 cgacgagcag gacctccagc agcagggcaa caccctgggc aagatcgtcg ccagccaccc
     4741 ggtggcgttc accgtgatcg ggttcgccgt gctgctggcg ctcagcctgc gcggcctgct
     4801 gttcgctccg gggatcgccg gcggcgcgct ggggctgttc cccgacagcg gccagacgct
     4861 gctgcgcgag ttcctggccg cgttccacca gaacgggctc ggctcgaccg ccgcggcccc
     4921 gccgaacttc gccgtgctcg gtgccctggc cacactgacc ttcaacaagg cgctgctggc
     4981 ggagaagctg ctgctgtggc tggcgctccc ggtggcggcg gcgacctgca cccgggcgct
     5041 gcgggtgctg gtcgactcgc ggcccgcgcg ggcgctcgcc ggcctgctgt atgccagctc
     5101 tccactggcg ttcggggcga tcgcccaggg gcggctcggc gagctgctgt tcctggtgct
     5161 ggcgccgccg acgctggcgc agctgccgct ggtgttccgc cccgaccagc cgcgggagtc
     5221 gtggcggccg gcgctgcgct tcatggtgct cgccgccgcc tcgatcgtgc tgtacccgcc
     5281 cgcgttcgtg gtgctggcgc tggtcgtggc ggtcgcggtg gtggcggcgc tggcgcgccc
     5341 ggcgcggggg ttgggccgct tcgcggtcaa gcaggccgtc ttcctggctg ccgcgatggg
     5401 cgggacgctg gtgctgctgg cgccgtggag cagcgagctg ctcgccgggc tgctgcgcgg
     5461 cgaccggccg gcagccgcgc cgaacctgct cgacctgctg cagctgcgcc cgggcgggcc
     5521 aggggtacca gggcatctgg tcgggccggt gtatccggcg ctggccctcg ccgcgatccc
     5581 gctggtgctg ccgacccggc ggctgcaggc gttctggctg gtggccgggt tcgttggtgc
     5641 ggcggtggtg gcggcctggg aggcgcacgg cctggcgccg ctgttcaccg actggcccgg
     5701 cggggtgctg gtggtcggcg cggtcgcctg ggccgccgcc gccgggatgg ccctgggccg
     5761 gctgcgggag ctgtgggagc aggcctcaag cctcgacccg cgccgcgtcg gggctggtgc
     5821 gctggcgctg ctgacgctgc tcacgggact gctcgtcacc gtccagttcg tgcgcggctc
     5881 gtgggcgccg ctgcaggcgg tcggccgccc ctcgctgccc gccacggtgc gccagagcag
     5941 cgcgcgcgtg ctgtggctcg ccggtcgccc cgaccacggg gtgaacttcg cggtgaccgg
     6001 gtccgacggg cgcaccctgc tcgacgacgg ccaggtcgcc tcggggaacg cggccggcgc
     6061 gctgcgttcg gtggtcaccg acgtggtcga ggcgcgcacc cacgcggccg ggcggctgct
     6121 gcagctgttc ggcatcggct acgtggtggt ccgccacggg cccgaggagg agcggctgac
     6181 cggcctggtc agccgccagc aggacctgcg cgcgcggccg accgagcaag ctgagctgtt
     6241 ccagggcccg accgtctccg cgagcatccc gagcgtcgcc ccggccgggt ggctgatcac
     6301 cagcgacacc ctgccgggca gcgtccaggg catgctcacc ggcccgctga tcgtcggcct
     6361 gcaagcggcg cagccgctgc tcagcggccc ggacgccaag ctgcccgccc acgtgatcag
     6421 cgacctgccc gccctgccgg gcggcgggcc cgccaccatc gtgctgcccg tccccgccga
     6481 ttccgcctgg caggcaacgg tcggcggcac ccgcctgcgg gcgggggtgg cgttcggctg
     6541 ggcgcaggcg ttccgcctcc ccgagggcgc cagtggcacc ctggacctgc gccgcaccgg
     6601 ccagacgagc cgcgaggcgc tgctgctggc tgagggcctg ctggtgctgg tcgcgctggc
     6661 ggcgatggcc cggccgacca gggtcgcccc gccggtcgcc ccgagcaccg tcgacgagac
     6721 caccggcgag ttccgcctca acacccggat ccgcgagggc gcccgatgag gaagaggatc
     6781 cgcgggggta gttcatgagc gacgacgacg accgcatgga gtacgacgac ttcgtctggg
     6841 acccttcaga gggcggccga cccccgggcc aggagcctga tccggcgccg tggcggcagc
     6901 cgttcagctg ggaaaccccc cagcagcccg agccggaacg cgacccgttc cgcgacaccg
     6961 ggccgctcga cccctacccg ccgatcggcg gtcccgccca gagcggcggt cccgccccgg
     7021 gcaaccgcga ccgccagcgc ttctggcgct ccgacccgga gccgccgctg ccgcccgacg
     7081 acggcggttc gctgaacggc cacaccaacg gccacaccaa cgggcacaac ggccacggcg
     7141 gcttcgacga cagcgatgcc ggagggcgct acgccgactt caccgaagcg ctgatcgagc
     7201 gcctcgccga cggcgtcgcc gatccccaca ccgacccgcg cctcccacgc cagccgcccc
     7261 cgccccag
//
LOCUS       DASWDC010000316         3983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_386865, whole genome shotgun sequence.
ACCESSION   DASWDC010000316 DASWDC010000000
VERSION     DASWDC010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386865"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VF995_10175"
     CDS             <1..307
                     /locus_tag="VF995_10175"
                     /inference="COORDINATES: protein motif:HMM:NF020940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEX9343966.1"
                     /translation="GVPVGEHERGPAAMTTTRRLVHCTLPPELVREPVLHGLVGASGA
                     VVNIHRANVDIEAGVAWYLLELSGVVEEVDRAEGWLQEQGIKVDRIADQRTGTDQGG"
     gene            489..1151
                     /locus_tag="VF995_10180"
     CDS             489..1151
                     /locus_tag="VF995_10180"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEX9343967.1"
                     /translation="MTEPPRHPDREILPPFAEIELPRRGKALRDLIVLRLIAIDRGIH
                     SLVFGAIAVGLIVLRYKLPLVQAEAQDVLNSLGAGLVNSGNPGRDFLVHLLERVLRLS
                     NRNLRTIVLIALGYMIVEGVEAFGLWWQYRWAEYLTVVATAGFVPFEIDELLKRVTVF
                     RVSALLINLAIVVYLVWAKRLFGLRGGEREDERLLEAAELLAPPPGIQAAQGSTAGSP
                     SG"
     gene            complement(1143..1778)
                     /locus_tag="VF995_10185"
     CDS             complement(1143..1778)
                     /locus_tag="VF995_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016905351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9343968.1"
                     /translation="MRTGFRMIVEAEPDITVVGQAGDGHEAVELAARVRPDVVLMDIR
                     MPRMDGIEATRRLAGPDARDPVKVLILTTFDLDELVVGALRAGASGFLLKDAPPEDLV
                     EAIRVVARGDALIAPSVTRRLLDRFAHSVMVEEAKPAVLDTLTARELEVLTLLARGLS
                     NAEIADHLVVSDTTVKTHVGRVLTKLDLRDRVQAVVFAYENGIVRPRSGSA"
     gene            complement(1799..3130)
                     /locus_tag="VF995_10190"
     CDS             complement(1799..3130)
                     /locus_tag="VF995_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX9343969.1"
                     /translation="MRPGALVEWVRRLPPTVADGLLAALVTAIALPSLVVGNPVYSQL
                     GIRFRSTNALAVLLVLAETVPLAWRRRAPVTVMAVSAGASMATLLAGFTPTAADLALI
                     IALYTVAAHTPRPKAVIVAFAGAALAFAEVTISAAKYGHTTWQDYLLFGVEIGATWFL
                     GDLQRRRRGYTAKLERLNRMLEARQSVARELIKRRTRQALAEERGRIARELHDVIAHS
                     VSVMVVQAGAARRVAGTDPEQARDAMTSIEEQGRQALTEMRRLVGMLRKGEGATAPDS
                     ESALAPQPSLQQVETLVEQAREGGLRVLLRIEGEVRPLPAAVDLSAYRIVQEALTNVR
                     KHAGTATAQVRLRYGPDELEVRVDDDGRGLAAVRSPRSDADGECEREGEGERDAGHGL
                     LGMRERVALFGGDLRVGPRPGGGFRVEARLPLEGARERERERERAAPWSAS"
     gene            complement(3241..3933)
                     /gene="clpP"
                     /locus_tag="VF995_10195"
     CDS             complement(3241..3933)
                     /gene="clpP"
                     /locus_tag="VF995_10195"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX9343970.1"
                     /translation="MADANLVPIVIEQTSRGERSFDIFSRLLKDRIIFLGTPIDDTIA
                     NLVMAQLLHLESDDPEKDISLYINSPGGSITDLLAIYDTMQYVKPDVSTICMGMAASA
                     AAVILSAGTKGKRYALPHSTILIHQPSGGARGQSADIEIQAREILRLRHLLDEIMAKH
                     TGQTVEKIARDTDRDFIMSADQAVEYGMIDEIITARQIVPVIASTSAPPGVSRPSGDG
                     AGPEPTAAPPAS"
BASE COUNT          529 a         1308 c         1555 g          591 t
ORIGIN      
        1 aggggttccg gtcggggagc atgaacgcgg gcctgcggcg atgacgacca cgaggcggct
       61 ggttcactgc acgctgccgc cggaactggt gcgggagccg gtgctgcacg ggctggtggg
      121 ggcgagcggg gcggtggtca acatccaccg tgccaatgtc gatatcgagg cgggggttgc
      181 ctggtacctg ctggagctgt cgggggtggt cgaggaggtg gaccgcgcgg agggctggct
      241 ccaggagcag gggatcaagg tggaccggat agccgaccag cggaccggga ctgatcaggg
      301 cggatagact ggggcacatg gggagacggc gccgtcgatg gcatccggag accctggtgt
      361 gttcgttcca gggtcaccag acgcctgcgg ctcgtgtggc ggggctgcgg ccggtggacc
      421 gggggcttgg gatcgacctg gacgacgggc ggcggttcgc gcgctgtatc cgctgtgacg
      481 cctgggttgt gaccgaaccg ccgcggcatc cggaccggga gatcctgccg ccgttcgctg
      541 agatcgagct gccccggcgc ggcaaggcgc tccgtgatct gatcgtgctg cggctgatcg
      601 cgatcgaccg ggggatccac tcgctggtgt tcggggcgat cgccgtcggg ctgatcgtgc
      661 tgcggtacaa gctgccgctg gtgcaggccg aggcgcagga cgtgctgaac agcctgggtg
      721 cggggctggt gaactccggc aacccgggca gggacttcct ggtgcatctg ctggagcggg
      781 tgctgcggct ctccaaccgc aatctccgga cgatcgtgct gatcgcgctc ggctacatga
      841 tcgtcgaggg ggtcgaggcg ttcgggctgt ggtggcagta ccgctgggcg gagtacctga
      901 cggtggtggc caccgccggg ttcgtgccct tcgagatcga cgagctgctc aagcgggtca
      961 ccgtgttccg ggtcagcgcg ctgctcatca acctggcgat cgtcgtctac ctggtgtggg
     1021 ccaagcggct gttcggtctg cgcggcggcg aacgcgagga cgagcgcctg ctggaggccg
     1081 ccgagctgct ggccccgccg cccgggatcc aggctgccca ggggagcaca gcaggctcgc
     1141 cgtcaggctg agcccgagcg gggccgcacg atgccgttct cgtaggcgaa caccaccgcc
     1201 tgcacccggt cgcggaggtc cagcttggtg aggacgcggc cgacgtgggt cttgacggtg
     1261 gtgtcgctga ccaccaggtg gtcggcgatc tccgcgttcg acaggccgcg ggcgagcagg
     1321 gtgaggacct ccagctcgcg agcggtgagg gtgtcgagca cggccggctt ggcctcctca
     1381 accatgacgc tgtgggcgaa ccggtcgagc agccggcggg tgaccgacgg ggcgatcagc
     1441 gcgtcgccgc gggcgacgac gcggatcgcc tccaccaggt cctcgggcgg ggcgtccttc
     1501 agcaggaagc cgctggcgcc ggcgcgcagc gcgccgacca ccagctcgtc gaggtcgaag
     1561 gtggtcagga tgaggacctt cacgggatcc cgcgcgtccg gcccggcgag ccgcctggtc
     1621 gcctcgatgc cgtccatgcg cggcatgcgg atgtccatca gcaccacgtc ggggcgcacc
     1681 cgcgcggcca gctcgaccgc ctcgtggccg tcgccggcct gccccaccac ggtgatgtcc
     1741 ggctcggcct ccacgatcat gcggaagccg gtgcgcatca gcggctggtc gtcgacgatc
     1801 aggacgcgga ccacggggcc gccctctcgc gttcgcgttc gcgttcccgg gcgccttcca
     1861 gcggcaggcg cgcctccacc cggaagccgc cgcccgggcg cggcccgacc cgcaggtcac
     1921 cgccgaagag ggccacccgc tcgcgcatgc cgagcaggcc gtgcccggcg tcgcgctcgc
     1981 cctcgccctc ccgctcacac tcgccgtcgg cgtcgctccg cgggctgcgg actgcggcga
     2041 ggccccggcc gtcgtcgtcg acccggacct ccagctcgtc ggggccgtag cggagccgca
     2101 cctgggcggt cgctgtcccg gcgtgcttgc ggacgttggt gagcgcctcc tgcacgatcc
     2161 ggtaggcgga caggtcaacg gcggcgggca gcggccggac ctccccctcg atccgcagca
     2221 ggacccggag gcccccctcg cgggcctgct cgaccagggt ctccacctgc tgcaggctcg
     2281 gctggggggc gagggcggac tcggagtcgg gcgcggtggc gccctcgccc ttgcggagca
     2341 tgccgaccag gcggcgcatc tcggtgaggg cctggcggcc ctgctcctcg atcgaggtca
     2401 tggcgtccct ggcctgctcg gggtcggtgc cggcgacgcg gcgcgcggcg ccggcctgga
     2461 ccaccatcac gctgaccgag tgggcgatca cgtcgtgcag ctcgcgggcg atgcggccgc
     2521 gctcctcggc gagcgcctgg cgggtgcggc gcttgatcag ctcgcgggcg accgactgcc
     2581 tggcctccag catccggttg agccgctcca gcttggcggt gtagccgcgg cggcggcgct
     2641 gcaggtcgcc gaggaaccag gtcgcgccga tctcgacgcc gaacagcagg taatcctgcc
     2701 aggtggtgtg gccgtacttg gcggcggaga tggtcacctc ggcgaaggcg agggcggcgc
     2761 cggcgaacgc gacgatcacc gccttggggc ggggggtgtg ggccgccacg gtgtagaggg
     2821 cgatgatcag ggcgaggtcg gcggcggtcg gtgtgaagcc cgccagcagc gtcgccatcg
     2881 aggcgccggc gctgaccgcc atgaccgtga ccggcgctcg ccgccgccag gccagcggca
     2941 cggtctcggc gagcaccagg agcacggcga gggcgttggt ggagcggaag cggatcccca
     3001 gctggctgta gaccgggttg ccgaccacca gcgaggggag cgcgatggcc gtgaccaggg
     3061 cagcgagcag cccgtcggcg acggtcggcg gcaaccgccg cacccattcg accaacgcac
     3121 ccggacgcat ggggcgacgg tagcaagcac caccccggac ggcatcatgc ccgcagatga
     3181 tccgcctctc ctcctcgcgg aggacggggc catccagaga ggtattcctg gtttcggagg
     3241 tcaggaggcg ggcggcgcgg cggtcggctc ggggccggcg ccgtcgccgg aggggcggga
     3301 caccccgggc ggggcgctgg tggaggcgat caccgggacg atctggcggg cggtgatgat
     3361 ctcgtcgatc atgccgtact ccacggcctg gtcggcgctc atgatgaagt cgcggtcggt
     3421 gtcgcgggcg atcttctcga ccgtctggcc ggtgtgcttg gccatgatct cgtccagcag
     3481 gtggcgcagg cgcaggatct cgcgggcctg gatctcgatg tcggccgact ggccgcgggc
     3541 gccgccggag ggctggtgga tcaggatggt cgagtgcggc agggcgtagc gcttgccctt
     3601 ggtcccggcg gagaggatca ccgcggcggc cgaggcggcc atgcccatgc agatcgtgga
     3661 gacgtcgggc ttgacgtact gcatggtgtc gtagatcgcc agcaggtcgg tgatcgaccc
     3721 gcccggcgag ttgatgtaca gcgagatgtc cttctccggg tcgtcgcttt ccaggtgcag
     3781 gagctgggcc atcaccaggt tggcgatggt gtcgtcgatc ggcgtgccga ggaagatgat
     3841 ccggtccttg agcagccggg agaagatgtc gaaggagcgc tcgccgcggc tcgtctgctc
     3901 gatgacgatg gggaccaggt tggcatcggc catgctgtca aaacctccgg cggaccgccg
     3961 cgtcggcgcg cacgtcgcgc tca
//
LOCUS       DASWDC010000317         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_387169, whole genome shotgun sequence.
ACCESSION   DASWDC010000317 DASWDC010000000
VERSION     DASWDC010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387169"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VF995_10200"
     CDS             complement(<1..908)
                     /locus_tag="VF995_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEX9343971.1"
                     /translation="MLRLDNAQFLAATRESTAAMKTTQAEGKGAYATLGAGAEKALKA
                     ISVASGIAAVASIALATKFDSAMERVHTQAGAAQAEVEKMGPALLDLAPKVATGPEAL
                     AESLFHIESVGLRGAKALDTVAASAKLAKVGNADLEQTTNAVAAAMVTQMKDARSLDQ
                     IIGLLNATVGQGNMKMNDLTAAISTGILPVAKNFGLGLADVGGALAVMTDRGMHADEA
                     ATRLKMTMTMMEAPTNKASKALHSVGLSSTQLAEDMHKPEGLVTALSDLKDHMDKAGL
                     SAIQQAQVISSVFGGGRSSAGVQTLM"
     gene            complement(1010..>1791)
                     /locus_tag="VF995_10205"
     CDS             complement(1010..>1791)
                     /locus_tag="VF995_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343972.1"
                     /translation="PGRPGRQRQAPERGREGLTVSFVSILFPDTGDFLTVADPKTEAG
                     QAAWERATAEAIEFSRNPGQAAMAKLVALVGRRSSLGDPTDPGFIENLSSSHRVVAME
                     QVAQVAATAHHLAGRNADGTRNPPIDGVVTMTPQGEAIISRPVTKASTADALRVAATQ
                     AGPQHADFAVRAAVAERETALRGKAAAPARQPTTTADLVPAQDLMLFKAARSGRRAAE
                     ADGLRARAAEYRALASVPGMPPPDTRTLLQAADELEQQAAG"
BASE COUNT          217 a          633 c          642 g          299 t
ORIGIN      
        1 ttcatcagcg tctgcacacc cgccgaggag cggccaccgc cgaacacgct ggagatgacc
       61 tgcgcctgct ggatcgcgga caacccggcc ttgtccatgt ggtccttcag gtccgacaag
      121 gcggtgacca ggccttccgg cttgtgcatg tcctccgcga gctgggtgct ggacaggccg
      181 accgagtgga gcgccttcga tgccttgttg gtcggcgcct ccatcatcgt catggtcatc
      241 ttgaggcggg tcgcggcctc gtcggcgtgc atcccgcggt ccgtcatcac ggcgagcgcg
      301 ccgcccacgt cggccaggcc caacccgaag ttcttcgcga ccgggaggat gccggtggag
      361 atcgcggcgg tcaggtcgtt catcttcatg ttcccttgac caaccgtggc gttgagcagg
      421 ccgatgatct ggtcgaggga gcgggcgtcc ttcatctggg tgaccatcgc cgcggcgacg
      481 gcgttggtgg tctgctccag gtccgcgttg ccgaccttcg cgagcttcgc cgaggcagcc
      541 acggtgtcga gcgccttcgc gccccgcagt cccacgctct cgatgtggaa caggctttca
      601 gccagcgctt ccgggccggt ggcgaccttc ggggccaggt cgagcagggc cggccccatc
      661 ttctccacct cggcctgcgc ggcgccggcc tgggtatgga cccgctccat tgccgagtcg
      721 aacttggtcg ccagcgcgat cgaggcgacc gccgcgatcc cgctcgccac gctgatcgct
      781 ttgagtgcct tctccgcccc agcgccgagg gtggcgtagg cgcctttccc ttcggcctgc
      841 gtcgtcttca tggcggcggt gctctcgcgg gtcgccgcga ggaattgggc gttatcgagc
      901 cgaagcaacc ccacaaccgg gggaagccag ccttcgccgg gcaccgatca gccctccacg
      961 ccggtcgcct gctccaccgc ggtggtcacc accaggcggg ccacggcggc taccccgctg
     1021 cctgctgttc gagctcgtcg gcggcctgca acagcgtgcg cgtgtcgggc gggggcatcc
     1081 cgggcacgct ggcgagcgct cggtattcgg cggcccgggc gcgcagcccg tcggcctccg
     1141 cggcgcgccg acccgagcgg gcggccttga acagcatcag atcctgcgcg ggcaccaggt
     1201 cggcggtcgt ggtgggctgc ctggctggcg cggcggcctt gccccggagc gcggtctccc
     1261 gctcggcgac ggcggccctg accgcaaagt cggcatgctg cgggccggcc tgggtggcgg
     1321 ccacgcgcag ggcgtctgcg gttgaagcct tggtgaccgg ccggctgatg atggcctcac
     1381 cctgcggcgt catcgtgacc acaccgtcga taggcgggtt gcgggtgccg tcggcgttgc
     1441 ggcccgccag gtgatgcgcc gtggccgcga cctgcgcgac ctgctccatt gcgacgacgc
     1501 ggtggctgga ggacaggttc tcgatgaagc cggggtcggt cgggtcgccg agcgagctgc
     1561 gccggccgac gagcgccacc agcttggcca tggccgcctg gccggggttc cggctgaact
     1621 cgatggcctc ggcggtcgcg cgctcccagg cggcctggcc ggcctcggtc ttcgggtcgg
     1681 ccacggtcaa gaaatcgccg gtgtcgggga acaggatgct cacgaacgac acggtcagcc
     1741 cctctcggcc acgctctgga gcctggcgct ggcgacctgg ccgacctgga g
//
LOCUS       DASWDC010000318         4262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_388294, whole genome shotgun sequence.
ACCESSION   DASWDC010000318 DASWDC010000000
VERSION     DASWDC010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388294"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VF995_10210"
     CDS             <1..211
                     /locus_tag="VF995_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9343973.1"
                     /translation="GAPARELLASSHSAFIHGMDVTVLTAAGVALLGALLALVFLPAR
                     AAQQAPAPAPIADAEGEPAAQIPVA"
     gene            219..893
                     /locus_tag="VF995_10215"
     CDS             219..893
                     /locus_tag="VF995_10215"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9343974.1"
                     /translation="MTQMAAPSAATTTPGQAPVREREPGRGPGRPRSAEAHQRILDAT
                     LQLLGEQGFGEMSVEAVATRAGVGKATIYRRWSSKEELVAAAIATLRPEVRLIDTGNV
                     RDDLIAVAREILRFKKAQASNFNVDLSWLMPRLSMETAKHPELHEAYQREFVRPHRRV
                     ITELIERAVARGELRDDLDPELLTDMLNGTFVYRAAIRCGLFQMDDDLIDRVIDLALE
                     GARPRS"
     gene            complement(1142..2335)
                     /gene="glgA"
                     /locus_tag="VF995_10220"
     CDS             complement(1142..2335)
                     /gene="glgA"
                     /locus_tag="VF995_10220"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEX9343975.1"
                     /translation="MRVALLTREYPPDVYGGAGVHVEYLARDLAKLVDLTVHCFGEPR
                     PPSTPPVAAYQSWDALNGPARQLSAMRTVSTDLAMAAGVEGADLVHSHTWYANLAGHL
                     GKLLYGIPHVATVHSLEPLRPWKAEQLGGGYELSKFCERIGLEGADRVIAVSDGMAAD
                     VLACYPSLDPAKVAVIRNGIDAEQYRPDPGTDVLERYGVDPERPTVIFVGRITRQKGV
                     PYLLDAAMRFDPAAQVVLCAGAADTAELGAEVAGKVNLLQAERSGVVWIEKMLQKREV
                     IQLLSHATLFVCPSIYEPLGIVNLEAMACEIAVVATATGGIPEVVVEGETGLLVPFTA
                     DAAGAPKDPVGFAAGIAERVNRLLADPALAERMGKAGRQRAVEHFGWDRIAAQVADLY
                     AELLG"
     gene            2579..3745
                     /locus_tag="VF995_10225"
     CDS             2579..3745
                     /locus_tag="VF995_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343976.1"
                     /translation="MAAGRFAALRRAGSFLALLASEIGAVGLLGRLGQVSALRIPWHH
                     LGPWLLNSPLQDVIGATLRMIALVAAWWLLSSNVLYLLASISRIPGAVLAVRWCTLPV
                     VRRVTDHAVALTLATSIMSAGAAAAIANATPASAATLPRRPGPVAANAASPYAPKPAG
                     PPGASLTPSPSPRPGTGPGTALGPGKPPAADPAAASPPGAQAQSVGGYLPHPAGNPSG
                     VTPAAPTTLPPPTYVPHPAGPPTAAPPRRHGTSSSSSTTSSTTPPPVTTPSATASSPT
                     TTTTPPTTAPPTTSPPSTTPPTTQPPAPVPSPSPAPSRTSTGQHEVTTGENLWSISRD
                     RLAAVTGRHPGDLSDHEIATYWLRVIEANQSHLRSHDPNLIFPGEIIHLPAVGG"
     gene            complement(3817..>4262)
                     /locus_tag="VF995_10230"
     CDS             complement(3817..>4262)
                     /locus_tag="VF995_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX9343977.1"
                     /translation="PGGRLCLRSMPPGDGARALIDWYVRSASAWLAPRGWSWTERIRA
                     TPSSIAVRDLGRRWGSAGQRGAVALHWALFQLPPALVDYVLVHELVHLDEPHHGKAFW
                     RRLDRALPDYQQRRARLADLGRHVWLGDVYVREPPTSPVPSTPGT"
BASE COUNT          625 a         1663 c         1391 g          583 t
ORIGIN      
        1 cggcgcgcca gcccgcgagc tgctggccag ctcccactcg gccttcatcc acggcatgga
       61 cgtgaccgtc ctgacggcgg ccggcgtcgc cttgctcggc gccctgctcg cgctggtctt
      121 cctgccagcg cgggccgccc agcaggcgcc ggcaccggcg cccatcgcgg acgcggaggg
      181 cgagccagcc gcgcagatcc ctgtggcatg atcaggagat gacccagatg gccgcaccct
      241 ccgccgcgac caccaccccc ggccaggccc ccgtccgcga acgggagccc ggtcgggggc
      301 ccggccgtcc ccgcagtgcc gaggcgcacc agcggatcct cgacgccacc ctgcaactgc
      361 tcggcgagca gggcttcggc gagatgagcg tcgaggcggt cgccacccgc gccggcgtcg
      421 gcaaggccac catctaccgc cgctggtcct ccaaggagga gctggtcgcc gccgcgatcg
      481 ccaccctgcg cccggaggtg cggctgatcg acaccggcaa cgtccgcgac gacctcatcg
      541 ccgtcgcccg cgagatcctc cgcttcaaga aggcccaggc cagcaacttc aacgtcgact
      601 tgagctggct catgccccgg ctctcgatgg agaccgccaa gcacccggaa ctccacgagg
      661 cctaccagcg cgagttcgtc cgcccccacc gccgggtgat caccgagctg atcgaacgcg
      721 ccgtcgcccg tggcgagctg cgcgacgacc tggaccccga gctgctcacc gacatgctca
      781 acggcacctt cgtgtaccgc gcggcgatcc gctgcggcct cttccagatg gacgacgacc
      841 tcatcgaccg ggtcatcgac ctcgccctgg aaggcgcccg cccgcgctcc tgaccaggca
      901 atgcaagatg atgtaggctt acatcgcggc gtggatgggg cgcgcgctgc cctgcgcgac
      961 ggcagtaagg cgggagagcg gcttgccaac cagtacctgc gttcccatcc tgacgtcgac
     1021 tccgttgact acgtgatcgc ggccacagct cagcacttgg gcgctgagct gtggacccgc
     1081 aacctgaagc actttccgat gttccctggg ctctcggccc cgtactgagc tggcgggttg
     1141 atcagccgag cagttcagcg tagaggtccg cgacctgggc ggcgatccgg tcccagccga
     1201 agtgctcgac ggcgcgctgg cggccggcct tgcccatgcg ctcggccagg gcagggtcgg
     1261 caaggaggcg gttgacccgc tcggcgatgc cggcggcgaa acccaccggg tccttggggg
     1321 cgccggcggc gtcggccgtg aaggggacca gcaggccggt ctcgccctcg acgacgacct
     1381 cggggatgcc gccggtggcg gtggcgacca cggcgatctc gcaggccatc gcctccaggt
     1441 tgacgatccc gagaggctcg tagatcgagg ggcagacgaa cagggtggcg tggctgagga
     1501 gctggatcac ctcgcgcttc tgcagcatct tctcgatcca gaccacgccg ctgcgctcgg
     1561 cctgcagcag gttgaccttc ccggccacct cggcgcccag ctcggcggtg tcggccgcgc
     1621 cggcgcacag caccacttgg gcggccgggt cgaagcgcat ggccgcgtcg agcaggtagg
     1681 ggacgccctt ctgccgggtg atgcggccca cgaagatgac cgtgggccgc tccgggtcga
     1741 cgccgtagcg ctccagcacg tcagtgcccg ggtcgggccg gtactgctcg gcgtcgatgc
     1801 cgttgcggat caccgcgacc ttggccgggt cgaggctggg gtagcaggcg agcacgtcgg
     1861 cggccatgcc gtcggagacc gcgatcaccc ggtcggcgcc ttcgagtcca atccgctcgc
     1921 agaacttcga cagctcgtag ccgccgccga gctgctccgc cttccagggc cgcagcggct
     1981 ccaggctgtg gacggtggcg acgtgcggga tgccgtagag gagcttgccg aggtgcccgg
     2041 cgaggttcgc gtaccaggta tggctgtgga ccagatcggc gccctcgacc cccgctgcca
     2101 tcgccaggtc ggtcgagacc gtccgcatgg cgctcagctg ccgggcggga ccgttgaggg
     2161 cgtcccagga ctggtaggcg gcgaccggcg gcgtgctggg cgggcgcggc tcgccgaagc
     2221 agtggacggt caggtcgacc agcttggcca ggtctcttgc caggtattcg acatgcacgc
     2281 cggcgccgcc atagacgtca ggcgggtatt cacgggtcag tagggccaca cgcacgaccg
     2341 gcccactcct ttcggttcgc tcattgttcg tgagaggtcc ccgggaaccg gggcggttag
     2401 tatccatcgc cgcgggcatc acgcgaacac cgcaggtgcg tgctggtgta gttgacatcc
     2461 tgtgctgcgt attccagact cgtggcgtac ttgtgcatat tgtccagatc ctcaaacctg
     2521 tagaaacgcg ggaacggcca aaccagcaac cgacgggact gaggggcgga gaggaacagt
     2581 ggcagccggt cgattcgcgg cgctccggcg cgcggggtcg ttcctcgcgc tgctggcgtc
     2641 cgagatcggc gcggtcgggc tcctcggccg cctcggccag gtctccgcgc tgcggatccc
     2701 ctggcaccac ctgggtccct ggctgctgaa cagcccgctc caggacgtca tcggcgcgac
     2761 cctgcggatg atcgccctgg tggcggcttg gtggctgctg tcgagcaacg tcctctacct
     2821 gctcgccagc atctcccgca tcccgggcgc ggtcctggcc gtgcgctggt gcaccctccc
     2881 ggtggtccgc cgggtcaccg accacgccgt cgccctcacc ctcgccacct cgatcatgag
     2941 cgcgggcgcc gccgctgcca tcgccaacgc caccccggcc tccgcggcga ccctgccgcg
     3001 ccgcccgggc ccggtcgccg ccaacgccgc ctccccctac gcaccgaaac ccgccggccc
     3061 tccgggcgcc tcactcactc cctccccttc gccccggccc ggaaccggac ccggaaccgc
     3121 actcggaccc ggaaagccgc ccgccgccga ccccgcggcc gcctcgccac ccggggcaca
     3181 ggcccaatcc gtgggcggct acctgcccca cccggccggc aacccctcgg gcgtcacccc
     3241 agccgccccg acaaccctgc ccccaccaac ctacgtgccc cacccagccg ggccccccac
     3301 agccgctcca cctcgtcggc acggcaccag ctccagttcc agcacgactt cgagcaccac
     3361 tcccccgccg gtcaccaccc cctcagccac ggcatcgtcg ccgacgacca ccaccacccc
     3421 accgacgacc gcaccgccga ccacgagccc gcccagcacc accccgccga ccacacagcc
     3481 gccagcgccc gtcccatcgc cgtccccggc gccctcccgc acgtccaccg gccaacatga
     3541 ggtgaccacc ggcgagaacc tctggtcgat ctcccgcgac cgcctcgccg ccgtcaccgg
     3601 ccgccacccc ggcgacctct ccgaccacga gatcgccacc tactggctcc gcgtcatcga
     3661 agccaaccag tcccacctgc gctcccacga ccccaacctg atcttccccg gcgagatcat
     3721 ccacctcccc gccgtcggcg gctgacgccg gagcacgatc acctctcggg ttgagcgcgt
     3781 ccccctcgac catgagcgcc cgtagcgcgt gactcctcag gtgcctggcg ttgaagggac
     3841 tgggctcgtg ggcggctcgc ggacgtagac gtcgccaagc cagacgtggc ggccgaggtc
     3901 ggcaaggcgc gcccggcgtt gctgatagtc gggaagggct cggtccaggc gtcgccaaaa
     3961 ggccttgccg tggtgcggtt cgtccaggtg aacgagctcg tggacgagga cgtagtcgac
     4021 cagcgcgggc ggcaactgga acaacgccca atgcagcgcc accgcgccgc gctgccccgc
     4081 gctgccccaa cgccgcccca ggtcgcggac cgcgatgctt gagggcgtgg ccctgatccg
     4141 ctcggtccag gaccagccgc gcggggcgag ccatgccgac gcgctgcgca cgtaccagtc
     4201 gatcagcgcc cgcgcgccgt cgcccggtgg catggagcgc aggcagagcc ggccacctgg
     4261 cg
//
LOCUS       DASWDC010000319         5874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1135131, whole genome shotgun sequence.
ACCESSION   DASWDC010000319 DASWDC010000000
VERSION     DASWDC010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135131"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /gene="larE"
                     /locus_tag="VF995_10235"
     CDS             complement(<1..741)
                     /gene="larE"
                     /locus_tag="VF995_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX9343978.1"
                     /translation="MDDELERKRGVLRQELKGAGGVMVAFSGGADSAFLLAAAVEALG
                     ERVVAATGVSPSLAAAELEDARGFAGELGVRHLCVPTHEGERPGYLRNGADRCYFCKD
                     ELFGVLGPLAELEGLALAVGANVDDLGEWRPGERAAAEHGVLTPLVAAGFSKAEVREA
                     SRRMGLRTAGKPAAACLASRVAYGVQVTPPRLSRIERAEALVRSFGVAELRVRDHGEL
                     ARVEVPVEWLPRLVEGELRERLAAGLREL"
     gene            complement(965..2581)
                     /gene="groL"
                     /locus_tag="VF995_10240"
     CDS             complement(965..2581)
                     /gene="groL"
                     /locus_tag="VF995_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180551.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9343979.1"
                     /translation="MPKIIAFDEDARRALERGMNQLADAVRVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELEEPYEKIGAELVKEVAKKTDDIAGDGTTTATVLAQALVREG
                     LRNVAAGANPMSLKRGIEKATELAVEAIKNQAKDVETRDEIAHTATVSAGDPEIGEVI
                     AKAMDAVGKEGVIQVEESNTFGIELETVEGMRFDKGYIAPHFVTDPERLEAVLEDPYI
                     LIANQKISAVKDLLPVLEKVMQTGKPVLIIAEDVDGEALATLVVNKIRGTFKSVAVKA
                     PGFGDRRKAMLGDIAILTGGQVISEEVGLKLENVSLDLLGHARRITVTKDETTVVEGA
                     GTEQDIKGRIAQIKTELENTDSDYDREKLQERLAKLAGGVGVIKVGAATEVELKEKKH
                     RIEDAVSNARAAVEEGIVPGGGVTLLRAQESLDKTDLTGDEATGASIVRRALEEPLKQ
                     ISINAGLEGGVVIEKVRSLEAGWGLNAASGEYVDLLKAGIIDPAKVTRSALQNAASIA
                     GLFLTTEAVVADKPEKEKAPAMPGGGDMDF"
     gene            complement(2694..3047)
                     /locus_tag="VF995_10245"
     CDS             complement(2694..3047)
                     /locus_tag="VF995_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9343980.1"
                     /translation="MLLASPRAPAVIQAVSEAEVIAPDHVNVEVLNALRRMERTGQIS
                     PARAAEAVSDLIGMHLRRLPTASLLARAWQMRAHVEIRDGCYVAAAQALDAPLVTSDL
                     RLSRAHGLGVAVITI"
     gene            complement(3067..3345)
                     /locus_tag="VF995_10250"
     CDS             complement(3067..3345)
                     /locus_tag="VF995_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343981.1"
                     /translation="MVKLVMSAIQVKNVPEELHVAIRRRAAEEGMSVGEYLLDLVRRD
                     LAVMSRRAWFDLIKTREPVLGVDVVKVLDEVRAEEDARIDEWIESRHH"
     gene            complement(3431..4735)
                     /gene="thrC"
                     /locus_tag="VF995_10255"
     CDS             complement(3431..4735)
                     /gene="thrC"
                     /locus_tag="VF995_10255"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX9343982.1"
                     /translation="MSENAPTTPALVPEPPAAFDHVQLRCRGCGTVYPAALEYACSKC
                     LGPLDAVYDPAVVAERLTRGSVEAGPRSVWRYGAILPAVPQDGDLAPGFTPLVAAPRL
                     AKALGIDGELWLKDETRNPTNSFKDRVAGVAIARARAFGLTTVGCASTGNLAGATAAA
                     AASQGLGCVVVIPAGLEVGKVAPTVACGATVVAVRGTYDQANRLASQAAEAFGWGIVN
                     VNLRPWYAEGSKTVGLEVAEQLGWSLPDHMVVPVASGALLCKVHQSFGLLADHGLVER
                     APVRVHGAQSDGCAPVATAFREGRDDVTPVKPSGIVKSLAIGDPADGPEVLQVARSTG
                     GRVESVSDGEVVESIELLARTTGIFGETAAGVTVATLNRLAAAGAFASGERVVALITG
                     HGLKTREALDGRADATVTVRPNIDALEGALAAERPELVGASG"
     regulatory      complement(4802..4940)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            5043..>5874
                     /locus_tag="VF995_10260"
     CDS             5043..>5874
                     /locus_tag="VF995_10260"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEX9343983.1"
                     /translation="MGDLDIMDWFGRRTFHYRQFEPVEQLRADRERLGASVSVCIPAR
                     NEAETIGEIVRCLRQEVLERARLIDEIVVMDGGSSDDTVAIARAEGAIAYREQEVLPE
                     AGPGAGKGEGLWKSLHVCRGDIICWVDADIRNIDPRFVYGLIGPLLRDPGIHYVKAFY
                     ERPIQQNGSLEESGGGRVTELVARPLINLFWPRLAGMVQPLSGEYAGRRAILEQVPFS
                     AGYGVELGLLVDIASRFGVDVIGQVDLERRVHRNQDMPALSRMSFGILQTALNRLEQD
                     G"
BASE COUNT          885 a         2124 c         1964 g          901 t
ORIGIN      
        1 cagctcgcgc agcccagcgg cgagccgctc ccgcaactcc ccctccacca gccgcggcaa
       61 ccactcgacc ggcacctcaa cccgcgccag ctccccatgg tcccgcaccc gcagctccgc
      121 caccccaaac gaccgcacca aggcctcagc ccgctcaatc cttgagagcc gcggcggcgt
      181 cacctgaacc ccgtaggcga cccgcgacgc caggcacgcc gcggccggct tgcccgccgt
      241 ccgcaacccc atccgccgcg acgcctcccg cacctccgcc ttggagaacc ccgcagccac
      301 cagcggcgtc agcaccccat gctccgccgc cgcccgctcc cccggccgcc actcccccag
      361 atcgtcgacg ttggctccca ccgccaacgc caaaccctcc agctccgcca gcggcccaag
      421 caccccaaac aactcatcct tacagaaata acaccggtcc gccccattcc gcagataccc
      481 cggccgctcc ccctcatgcg tcggcacaca cagatgccgc acccccagct cccccgcgaa
      541 cccccgcgcg tcctccaact cagcagccgc caaactcggc gacacccccg tcgccgccac
      601 cacccgctcc cccagcgcct caaccgcagc agccagcaaa aacgccgagt ccgccccacc
      661 agagaacgcc accatcaccc cgccagcgcc cttcagctcc tgacgaagga ccccgcgttt
      721 gcgctccagc tcatcatcca tgcaggccaa tctagcaggc gtagcaccga cgagttgcaa
      781 tgcctgcttg aggccagcac cccccaaccg tcggctgcgt tgtcataggg tgagggcgag
      841 accccgccag ccacggagcc cggggcaggg tcccactcgt gcgacctggt tccgtagcaa
      901 cgagcgggac cctgccccgg gcgaccaacg tcagcccggc gcagggtccc gcccccgggt
      961 gctcctaaaa gtccatgtcg ccgccgccgg gcatggcggg ggccttctcc ttctcgggct
     1021 tgtcagccac cacggcctca gtggtcagga acagccccgc gatcgaagcg gcgttctgca
     1081 gcgccgagcg ggtcaccttg gcggggtcga tgatgccggc cttgagcagg tcgacgtact
     1141 cgccgctggc ggcgttcagg ccccagccgg cttccaggct gcggaccttc tcgatcacga
     1201 cgccgccttc gaggccggcg ttgatggaga tctgcttgag gggctcttcg agcgcgcggc
     1261 gcacgatcga cgcgccggtc gcctcgtcgc cggtcaggtc ggtcttgtcg aggctctcct
     1321 gggcgcgcag gagggtgacg ccgccgccgg ggacgatgcc ctcctcgacc gcggcgcggg
     1381 cgttggagac ggcgtcctcg atgcggtgct tcttctcctt cagctcgacc tcggtggccg
     1441 cgccgacctt gatgacgccg acgccgccgg cgagcttggc gagccgctcc tgcagcttct
     1501 cgcggtcgta gtcggagtcg gtgttctcca gctcggtctt gatctgggcg atgcggccct
     1561 tgatgtcctg ctcggtgccg gcgccctcga caaccgtggt ttcgtccttg gtgaccgtga
     1621 tccggcgggc atggccgagg aggtccagcg agacgttctc gagcttgagg ccgacctcct
     1681 cgctgatgac ctggccgccg gtgaggatgg cgatgtcacc gagcatggcc ttgcggcggt
     1741 cgccgaagcc gggggccttg accgcgaccg acttgaaggt gccgcggatc ttgttgacca
     1801 ccagggtggc cagcgcctcg ccgtcgacgt cctcggcgat gatcagcacc ggcttgccgg
     1861 tctgcatgac cttctccagc accgggagca ggtccttgac cgccgagatc ttctggttgg
     1921 cgatcaggat gtaggggtcc tcgagcaccg cttcgagccg ctcggggtcg gtcacgaagt
     1981 ggggagcgat gtagcccttg tcgaagcgca tcccctcgac ggtctccagc tcgatgccga
     2041 aggtgttgga ctcctcgacc tggatcacgc cctccttgcc gacggcgtcc atcgccttgg
     2101 cgatgacctc gccgatctcg gggtcgccgg cggagacggt ggcggtgtgg gcgatctcgt
     2161 cgcgggtctc gacgtccttg gcctggttct tgatggcctc gacggccagt tcggtggcct
     2221 tctcgatgcc gcgcttgagg ctcatcgggt tggcgccggc ggcgacgttg cgcagcccct
     2281 cacggaccag ggcctgggcc agcacggtcg ccgtggtggt gccgtcaccg gcgatgtcgt
     2341 cggtcttctt ggcgacctct ttgaccagct cggcgccgat cttctcgtag ggctcctcga
     2401 gctcgatctc cttggcgatc gagacgccgt cgttggtgat cgtgggcgca ccccacttct
     2461 tctcgaggac cacattgcgg cccttgggcc ccaacgtcac ccgcaccgcg tccgcgagct
     2521 ggttcatccc ccgctcgagc gcccgccgag cgtcctcatc gaaggcgatg atcttgggca
     2581 tgcgcgacta cctcctattg gtggcttgcc cgtcagatat acgactatgg ccccagcggg
     2641 ccgggggtat gttagcactc ctccaaggtg agtgctaacc gttgcgtcaa gggtcagatc
     2701 gtgattaccg ccacgccaag cccgtgggcg cgggaaaggc gaaggtcgct ggtgaccagc
     2761 ggagcatcga gcgcctgagc tgcggcgacg tagcatccgt cgcggatctc gacgtgagcg
     2821 cgcatttgcc aggcccgcgc cagcagagat gcggtcggaa ggcgacgaag gtgcatccca
     2881 atgaggtcgc tgaccgcctc cgcagcacgc gcggggctga tctgcccagt ccgctccata
     2941 cggcgcagag cgttcagaac ctcaacgttg acgtgatcgg gggcaatcac ctctgcctcg
     3001 ctgacagctt ggatcaccgc gggcgcgcga gggctggcga ggagcaactc gaccagggca
     3061 gatgcgtcaa tgatggcggg actcgatcca ctcatcaatc ctggcgtcct cctccgcccg
     3121 cacctcgtcg agcaccttga cgacgtcgac ccccaagacg ggctctctgg tcttgatcag
     3181 gtcgaaccat gctcggcggc tcatcaccgc gaggtcccgg cggacaaggt cgagaaggta
     3241 ttcaccgaca ctcatgccct cctcagcagc acgacgccgg atcgccacgt gaagctcctc
     3301 tgggacgttc ttgacctgga ttgcgctcat tacgagcttc accaccctag catgccggaa
     3361 gcatgccaga agcatgccag aagcatctgt ggataactac catcgggcga aataccgcgc
     3421 cgagccctgg tcagccgctg gcgccgacca actccgggcg ctccgccgcc agggcccctt
     3481 ccagggcgtc gatgttgggg cggacggtga cggtggcgtc ggcgcggccg tcgagggcct
     3541 cgcgggtctt caggccgtgg ccggtgatca gggcgacgac gcgctccccc gaggcgaacg
     3601 ctccggcggc ggcgaggcgg ttgagggtgg caacggtgac gccggcggcg gtctcgccga
     3661 agatgccggt ggtgcgggcg agcagctcga tcgactcgac cacctcgccg tcgctcaccg
     3721 actcgacccg gccgccggtg gagcgggcga cctggaggac ctccggcccg tcggcggggt
     3781 cgccgatcgc cagcgacttc acgatgccgc tgggcttgac cggggtgacg tcgtcgcggc
     3841 cctcgcggaa ggcggtggcg accggggcgc agccgtcgga ctgggcgccg tggacccgga
     3901 cgggggcgcg ctccaccagg ccgtggtcgg cgagcaggcc gaagctctgg tggaccttgc
     3961 agagcagggc gccggaggcg accgggacga ccatgtggtc gggcagcgac cagccgagct
     4021 gctcggcgac ctccaggccg acggtcttgg agccctccgc ataccagggg cgcaggttga
     4081 cgttgacgat cccccagccg aacgcctcgg cggcctgcga ggcgagccgg ttggcctggt
     4141 cgtaggtgcc ccgcacggca acgaccgtgg cgccgcaggc gaccgtcggc gcgaccttgc
     4201 cgacctccag gcccgccggg atcaccacca cgcagccgag accctgggag gcggccgccg
     4261 cggcggtggc gccggccagg ttgccggtgg acgcgcagcc gacggtggtc aggccgaagg
     4321 cgcgggcgcg ggcgatcgcg acgccggcca cccggtcctt gaaggagttg gtggggttgc
     4381 gggtctcgtc tttcagccac agctcgccgt cgatgccgag cgccttggcc aggcgcggcg
     4441 cggccaccag cggcgtgaac ccgggcgcga ggtcgccatc ctggggaacc gccggcagga
     4501 tcgccccgta gcgccagacc gagcgcggcc ccgcctcgac cgagccgcgg gtcagccgct
     4561 cggccaccac cgccgggtca tacacggcgt ccagcggacc aaggcacttg gagcaggcgt
     4621 attcgagggc ggcgggatag accgtgccgc agccgcggca gcggagctgg acgtggtcga
     4681 atgcggcggg gggttcggga acaagggcgg gggtggtggg cgcgttctcg gacaaggccg
     4741 gtgaccctcc ttcagggttg gctcgttggc gaacgccacg cgccgcggtc accggaccgc
     4801 tcgcgcatct ttccccgccg gcttgggtac accaccctcg tcggctggga cggacgtggc
     4861 accttcccct ggagagtcgc cctcccaggg aggttgccgg ggcttcgtcg ggccggtccc
     4921 tccacccctc ttgatgcgtc gccagtactc ttcaattgtc accgtccagc ttgccttcgc
     4981 atgcgccgaa cggtcaacgt cagcagcaac agccagtgga taaggagtag ataccgggcc
     5041 gaatgggaga tctcgacatc atggactggt tcggtcgccg aaccttccac tacaggcagt
     5101 tcgagccggt cgagcagctt cgcgccgacc gggaacgact cggggcgagc gtgtcggtct
     5161 gcatccccgc ccgcaacgag gccgagacca tcggtgagat cgtgcgatgc ctccgccagg
     5221 aggtgctgga gcgcgcccgg ctgatcgacg agatcgtggt gatggacggg ggctccagcg
     5281 acgacaccgt ggccatcgcc cgtgccgagg gcgccatcgc ctaccgcgag caggaggtcc
     5341 tgcccgaggc cggcccgggc gccggcaagg gcgagggcct ctggaagagc ctgcatgtct
     5401 gccgcggcga catcatctgc tgggtcgacg ccgacatccg caacatcgac ccacgcttcg
     5461 tgtacgggct gatcgggccg ctgctgcgcg acccgggcat ccactacgtg aaagccttct
     5521 acgagcgccc catccagcag aacggcagcc tcgaggagag cggcggcggg cgtgtcaccg
     5581 agctggtcgc gcggccgctg atcaacctgt tctggccgcg gctcgccggg atggtgcagc
     5641 cgctctccgg cgagtacgcc ggacgccggg cgatcctgga gcaggtgccg ttctcggccg
     5701 gctacggcgt cgagctgggc ctgctggtgg acatcgccag ccgcttcggc gtcgacgtga
     5761 tcggccaggt cgacctggag cgccgggtcc accgcaacca ggacatgccg gcgctgtcgc
     5821 ggatgagctt cggcatcctg cagaccgccc tcaaccgcct ggagcaggac ggac
//
LOCUS       DASWDC010000320         5451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_972525, whole genome shotgun sequence.
ACCESSION   DASWDC010000320 DASWDC010000000
VERSION     DASWDC010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972525"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VF995_10265"
     CDS             complement(<1..551)
                     /locus_tag="VF995_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX9343984.1"
                     /translation="MSPPSGRPALTRHFLGLQQVPQDLEATVVTIGMFDGVHRGHQAL
                     LGRVVEEAAARGLPPAAITFDRHPMEVLRPGSHPRLLTTLRQKVALLDAAGMAFVLVL
                     PFTLELSRVPAEEFAAQVLFGAMRARAVVVGANFRFGHKATGDLGLLTELGQRRGVEV
                     LGVPLQALDASGGREPSAGEAVSS"
     gene            complement(548..1465)
                     /gene="truB"
                     /locus_tag="VF995_10270"
     CDS             complement(548..1465)
                     /gene="truB"
                     /locus_tag="VF995_10270"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX9343985.1"
                     /translation="MGVALDGVLVCDKPAGMTSHDVVARVRRIAGQRRVGHGGTLDPP
                     ATGVLVVALGRATRLLPFLPTEPKRYLATIAFGTETDTLDADGTVTATAPTGQIHQEG
                     MAAALARLIGPQLQVPPMVSAVKLGGERLYAKARRGETVDRAPRPITIHALDLLRFED
                     QGSPAPRATVAVTCSGGTYVRVLAADLGRAPGSLAHLASLRRTAVGAFTEDAAHALDQ
                     LEGAALQLLLLSPADAMGLGMGASDGAAPAAAPARALSDQEAAALATGKSIDPSGRAE
                     PVAAIAPDGHLVAVIQDGAGRARPRVVLE"
     gene            complement(1471..2451)
                     /locus_tag="VF995_10275"
     CDS             complement(1471..2451)
                     /locus_tag="VF995_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX9343986.1"
                     /translation="MTIEVQEQDWQAAVAAIAEAEQAVISCHVHPDGDALGSALALHR
                     ALANDGRKPVVSFSEPFTVARQYRFLPGVEDLTPPDQVPRAAELFVCFDAGSLDRLGT
                     LVDAFHGAARTVVIDHHASNTRFGDVNLVDPSAPASAVLCRELLRRLSLPLDPRIATN
                     LYTALVTDTGRFQYQSTVPATHELAAELLAAGVKQYEVAKAVFETNDIGYLRLLADVL
                     ARIQQVSAASLVWTWVTQADLAAHGIDMEQTEGLIDLVRTDGVSDVAAVLKEQPDGAF
                     KVSLRSKGGANVGRLAAAEGGGGHALAAGFTSELGVEATIQRIVAALQAA"
     gene            complement(2448..2894)
                     /gene="rbfA"
                     /locus_tag="VF995_10280"
     CDS             complement(2448..2894)
                     /gene="rbfA"
                     /locus_tag="VF995_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX9343987.1"
                     /translation="MAKPSYPRARRLAETVRRLVTEWLEQERAAERQHGLPSGLLTVT
                     DVRVTPDLHHATVFYTVLGGEEQAGSDKELAEATPRARSYVAQHLRLRHAPTLEFVSD
                     DVPGHGAKIDRLLAEIQRHEPGADTPDSGSAAPDDEQPPGQEGSQR"
     gene            complement(2898..3191)
                     /locus_tag="VF995_10285"
     CDS             complement(2898..3191)
                     /locus_tag="VF995_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX9343988.1"
                     /translation="MFVALLQLDLRLPDATSLKDKRSVVKGLVAGLRRLNCSVAEVSH
                     QDLRQRATLAVAAVAEEGFHARRVLVSAEREVERTPGVELLSSHVTVYGPEDA"
     gene            complement(3231..4949)
                     /gene="infB"
                     /locus_tag="VF995_10290"
     CDS             complement(3231..4949)
                     /gene="infB"
                     /locus_tag="VF995_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX9343989.1"
                     /translation="MGRPAGDLIRILMQMGEMLTATQSMSDDAVTLLADEIGARIEVV
                     DPADADAFQQIQGWEDAQADRDEDLVPRPPVVTVMGHVDHGKTKLLDAIRHTDVVAGE
                     AGGITQHIGAYQVHAGEDGRLVTFIDTPGHEAFTAMRARGAAVTDIAVLVVAADDGVM
                     PQTVEAINHAKAAGVPIVVAVNKVDKPEANPIRVRQQLTEYDLVAEEYGGKTQFVDVS
                     AKANLNLDTLVETILLVADVELNLKANPSRRAQGTIIEAHLDKGRGPVATILVRRGTL
                     HVGDAIAAGTTFGRVRAMFDENGKEVDAALPAQPVQVLGLSAPPSAGDEVRAVADERV
                     ARSIAQTREARQRQAEIASTRAPRLEDLFAQIKEGQLQELPLVIKADVMGSVEAIDDA
                     LNKLDVPEVRMRVLQKGAGAITENDVSLAAASKAIIIGFSVRPTPAARELAIREGVDI
                     RLYSVIYQVVEDVDRALKGLLAPEFEERPLGTAEVRQLFRVPRIGVIAGSYVLSGDIT
                     RGAKARLVRDGVVVYDGRIDSLRRFKDDVRTVATGYECGIGLENYSDVHERDQIEAYE
                     MVEIPR"
     gene            complement(4925..>5451)
                     /locus_tag="VF995_10295"
     CDS             complement(4925..>5451)
                     /locus_tag="VF995_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343990.1"
                     /translation="VQVVVPEAPLGVPAWARVQASPAEPVAAAPAWVRVLVRRGSVLV
                     RRGSVLARRGSVLARRVAGVLVVLAAAVRLGRAVAAVPDSAAVPVAAARPGLAAALPA
                     VVVLVVAAAPSAGSAARGRSAAGAAGAVRNSSRWARPSWSSVLRSRSRASFGSRAASP
                     CRTWPTAWAGPPAT"
BASE COUNT          714 a         1905 c         2057 g          775 t
ORIGIN      
        1 gaggagaccg cctcgccagc ggagggttcg cgcccccccg atgcgtcaag ggcctggagc
       61 gggacgccga gcacctcgac gccgcgccgc tggcccagct cggtgagcag cccaagatcg
      121 ccggtggcct tgtggccgaa gcggaagttg gcgccgacca ccaccgcccg cgcccgcatc
      181 gcgccgaaca gcacctgtgc ggcgaactcc tccgccggga cgcgcgacag ctccagggtg
      241 aagggcagca ccaggacgaa ggccattccg gccgcgtcga gaagcgccac cttctggcgc
      301 agggtggtaa gcagccgcgg gtggcttccg gggcgcagca cctccatggg atggcggtcg
      361 aaggtgatcg cggccggcgg cagcccgcgg gccgccgcct cctcgacgac ccggccgagc
      421 agcgcctggt ggccccggtg cacgccgtcg aacatcccga tggtgaccac ggtcgcctcc
      481 aggtcctgcg ggacctgctg gagtccgagg aaatgtcgcg tcagcgccgg cctgcccgat
      541 ggggggctca ttcgaggacg actcgaggtc gggcgcggcc ggcgccatcc tggatgaccg
      601 cgaccaggtg gccgtccggg gcgatcgccg cgaccggttc ggcgcggccg ctggggtcga
      661 tggacttgcc ggtggccagc gccgccgcct cctgatcgga gagcgcccgc gccggggcgg
      721 ccgccggggc ggcgccgtcg ctcgcgccca tgcccaggcc catggcgtcg gcgggtgaca
      781 gcagcagtag ttggagcgcc gcgccctcca gctgatcgag ggcatgggcg gcgtcctcgg
      841 tgaacgctcc gaccgctgtg cggcgcagcg aagcgaggtg ggcgaggctg ccgggggcgc
      901 ggccgaggtc ggcggcgagg acgcggacat aggtaccgcc ggagcaggtc accgcgacgg
      961 tcgcccgcgg cgccggactg ccttggtcct cgaagcggag caggtcgagc gcgtggatcg
     1021 tgatcgggcg gggggcgcgg tccacggtct cgccgcggcg agccttggcg tagaggcgct
     1081 cgccaccgag cttgaccgcc gacaccattg gcgggacctg gagctggggt ccgatcagcc
     1141 gggccagtgc cgcggccatg ccctcctggt ggatctggcc ggttggcgcg gtcgcggtca
     1201 cggtgccgtc ggcgtcgagg gtgtcggttt ccgtcccgaa cgcgatcgtc gccaggtagc
     1261 gcttgggctc ggtcggaagg aacggcagca ggcgggtggc gcggccgagc gcgacgacca
     1321 gcacaccggt ggcaggcggg tcgagcgtgc cgccgtgccc gactctgcgt tggccggcga
     1381 tccggcgaac gcgggcgacc acgtcgtggg aggtcatccc cgctggcttg tcgcagacaa
     1441 gcacgccgtc caaggccact cccaccctac ctacgccgcc tggagggcgg cgacgatccg
     1501 ctggatggtg gcctcgacgc ccaactcgga ggtgaagccg gcagcgaggg cgtggccgcc
     1561 gccgccttcg gccgccgcga ggcggccgac gttggcgccc cccttggagc gcagcgacac
     1621 cttgaacgcg ccgtcgggct gctccttgag caccgcggcg acgtcggaga cgccgtcggt
     1681 gcggaccagg tcgatcaacc cctcggtctg ctccatgtcg atgccgtgag cggccaggtc
     1741 ggcctgggtg acccaggtcc agaccaggga ggcggccgag acctgctgga tgcgtgcgag
     1801 cacgtcggcg agcaggcgca ggtagccgat gtcgttggtc tcgaagaccg ccttggcgac
     1861 ctcgtactgc ttgacgcccg ccgccagcag ctcggcggcc agctcgtggg ttgccggcac
     1921 ggtggactgg tactggaagc ggccggtgtc ggtaaccagt gcggtgtaca ggttggtggc
     1981 gatccgcggg tcgagcggga ggctgaggcg gcgcagcagc tcgcggcaca gcaccgcgct
     2041 ggccggtgcg gacgggtcga ccaggttgac gtcgccgaag cgggtgttgg aggcgtgatg
     2101 gtcgatcacg accgtccggg cggcgccgtg gaaggcgtcg accagggtgc ccagccggtc
     2161 gagcgagccg gcgtcgaagc agacgaacag ctcggcggcg cggggcacct ggtcgggtgg
     2221 ggtgaggtcc tcgacgcccg gcaggaagcg gtactgccgc gccacggtga acggctcgga
     2281 gaagctcacc acgggcttgc ggccgtcgtt ggcgagcgcc cggtgcagcg ccagcgccga
     2341 gccgagggcg tcgccgtcag gatgcacgtg gcaggagatc acggcctgct cggcctcggc
     2401 gatcgcggcg accgcggcct gccagtcctg ctcctgcacc tcgatggtca tcgctgcgag
     2461 ccttcctggc cgggtggctg ctcgtcgtcg ggtgcggcgc tgccactgtc cggggtgtca
     2521 gccccgggtt cgtgccgctg gatctcggcg agcagccggt cgatcttcgc gccatgtcct
     2581 ggcacgtcgt cggagacgaa ttctagcgtc ggcgcgtgcc gcagccgcag gtgttgtgcc
     2641 acgtagctgc gcgcccgcgg ggtcgcctcg gccagctcct tgtcgctccc ggcctgctcc
     2701 tccccgccga ggaccgtgta gaagaccgtc gcgtggtgca ggtccggggt gacgcggacg
     2761 tcggtgacgg tcagcagccc ggacgggagc ccgtgctggc gctcggcggc gcgctcctgt
     2821 tcgagccatt cggtgaccag gcgcctgacc gtctcggcaa ggcgcctggc acgtgggtag
     2881 cttggcttgg ccatggttca ggcgtcctcc ggcccgtaca ccgtcacgtg gctggagagc
     2941 agctccacgc cgggggtgcg ctcgacctcg cgttcggcgc tgaccagcac cctgcgggca
     3001 tggaatccct cctcagccac ggctgccacc gccagggtgg cacgctgccg caggtcctgg
     3061 tggctgacct ccgcgactga gcagttcagc cggcggaggc cggccaccag gcctttgacc
     3121 accgagcgct tgtccttcag cgaggtcgcg tcgggcaggc gcaggtccag ttgcagcagg
     3181 gcgacgaaca cgggtcaggt tcagcgtcag gttgagggtc agatttcggg tcagcgtggg
     3241 atttccacca tctcgtaggc ctcgatctgg tcgcgttcgt ggacgtcgct gtagttctcc
     3301 aggccgatgc cacactcgta gccggtcgcg acggtacgga cgtcgtcctt gaagcgtcgc
     3361 agcgagtcga tgcgcccgtc atagacgacc acgccgtcac ggaccaaccg cgccttggcg
     3421 ccgcgggtga tatcgccgga gaggacgtag gagccggcga tcacgccgat gcgcggcacc
     3481 cggaacagct gccggacctc ggcggtgccg agggggcgct cctcgaactc gggggcgagc
     3541 aggcccttga gcgccctgtc gacgtcctcg accacctggt agatgaccga gtacaggcgg
     3601 atgtcgacgc cctcgcggat ggccagctcc cgggcggccg gcgtgggccg caccgagaag
     3661 ccgatgatga tcgccttgct ggccgcggcc aggctgacgt cgttctcggt gatcgcgccg
     3721 gcgcccttct gcagcacacg catgcggacc tcggggacgt cgagcttgtt gagcgcgtcg
     3781 tcgatcgcct cgaccgaacc catcacgtcg gccttgatca ccagcggcag ctcctggagc
     3841 tggccctcct tgatctgggc gaacaggtcc tccaggcggg gtgcccgggt ggacgcgatc
     3901 tcggcctggc gctggcgcgc ctctcgggtc tgcgcgatcg agcgggcgac ccgttcgtcg
     3961 gcgaccgcgc ggacctcgtc gccggcgctc ggcggcgccg agaggccgag gacctgcacc
     4021 ggctgggccg gcagcgcggc gtcgacctcc ttgccgttct cgtcgaacat ggcgcgcact
     4081 cggccgaagg tggtgccggc ggcgatcgcg tcgccgacgt gcagggtgcc gcgccggacc
     4141 aggatggtcg cgaccggacc gcggcccttg tccaggtgcg cctcgatgat ggtgccttgg
     4201 gcgcggcggc tggggttcgc cttgaggttg agctcgacgt cggcgaccag caggatcgtc
     4261 tcgaccagcg tgtcgaggtt caagttggcc ttggcggata cgtcgacgaa ctgggtcttg
     4321 ccgccgtact cctcggcgac caggtcgtac tcggtgagct gctggcggac ccggatcggg
     4381 ttggcctcgg gcttgtcgac cttgttgacc gccaccacga tcggcacccc ggcggccttg
     4441 gcgtggttga tcgcctccac tgtctgcggc atcacgccgt cgtcggcggc gaccaccagc
     4501 acagcgatgt cggtgacggc ggcgccgcgg gcacgcatgg cggtgaacgc ctcgtggccc
     4561 ggggtgtcga tgaaggtgac caggcggccg tcctcaccgg cgtggacctg gtaggcaccg
     4621 atgtgctggg tgatgccgcc agcctcgccc gccaccacgt ctgtgtggcg gatcgcgtcg
     4681 agcagcttgg tcttgccgtg gtcgacgtgg cccatgacgg tgaccaccgg tgggcgcggg
     4741 accaggtcct cgtcccggtc cgcctgggcg tcctcccagc cctggatctg ctggaaggcg
     4801 tcggcgtcgg ccgggtcgac cacctcgata cgggcgccga tctcgtcggc gagcagggtg
     4861 acggcgtcgt ccgacatcga ctgggtggca gtgagcatct cgcccatctg catcaggatg
     4921 cggatcaggt cgccggcggg ccggcccatg cggtcggcca ggtcctgcac ggtgacgccg
     4981 cgcgggaccc gaatgaggcc ctcgaccgag atctcaggac gctgctccag ctggggcgcg
     5041 cccatcgact cgaattcctc acggcgcctg ccgcccctgc ggcgcttcgg ccgcgggccg
     5101 cggaacccgc cgaaggggcg gccgccacca ccaggaccac cacggccggc agggccgccg
     5161 cgagacccgg gcgggccgcc gccaccggga cggccgccga atccgggacg gccgccaccg
     5221 cccggcccag ccggacggcc gccgccagga ccaccaggac gcccgccacc cggcgcgcca
     5281 ggacgctgcc ccggcgcgcc aggacgctgc cccggcgcac caggacgctg ccccggcgca
     5341 ccaggacgcg gacccatgcc ggggcggccg ccaccggctc cgccgggctg gcctggacgc
     5401 gggcccatgc cgggacgccc agcggggcct ccggcaccac cacctggacg g
//
LOCUS       DASWDC010000321         4765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_392945, whole genome shotgun sequence.
ACCESSION   DASWDC010000321 DASWDC010000000
VERSION     DASWDC010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392945"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="VF995_10300"
     CDS             complement(<1..1182)
                     /locus_tag="VF995_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX9343991.1"
                     /translation="MIAVLALGFTLFALASPSGMRAAGNFPAQFLLFAALILVGEHMP
                     LELPAGEGGDLATMAQPFAFALLLGWGIAAAVLAFGAASLLAGTVGRKPILRVLVATA
                     KGILALAGAGIVWRLLGGPKGPSLTELPAFATAAATFLLLANLLPASASALGTGAARG
                     PGARAAGSGSGRGQQLWTSALLLALAPVMVAIADRYLALIPLLGLPMVALYRTARSAQ
                     RAELERARAEAAAEANAAIAAEQARMIQGKQALVRQLQEQDRLKHDLLATVSHELRNP
                     LTVVLGSLDTLSAHVGELGPDEREELVDLASRQGRRLKLLVDQLLLSSHEAEATGQPG
                     DVDGNGTQPQVPASDGAQVVREAVSAARVCHPTRPIEILVDDTLPLHAAAEPILQVLT
                     NL"
     gene            1470..1985
                     /locus_tag="VF995_10305"
     CDS             1470..1985
                     /locus_tag="VF995_10305"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HEX9343992.1"
                     /translation="MPLFGGRLSLLADAHVRLAGVLPLALALQVLAVNVPGVPQHLRP
                     VLQVASYPVGLVFVAANWRLAGIPLVGLGALGNLAAILANGGTMPATAAALASAGLAA
                     HPHRYANSAAVAHPRLAFLGDLFSVPRRFPLHNVFSVGDLLIALGVVVAVHALCGSRP
                     RLRLRGQRQKA"
     gene            2072..2992
                     /gene="mca"
                     /locus_tag="VF995_10310"
     CDS             2072..2992
                     /gene="mca"
                     /locus_tag="VF995_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HEX9343993.1"
                     /translation="MSRILLAVHAHPDDESSKGAGTLARYADQGVRTVIVTCTGGEAG
                     EILNPAMDKPGNLERMTELRREELAKALEILNVSAHYFLGYRDSGMPDTETNAHPDAF
                     VNAPLDEAIGRLVRIIREERPQVLVTYDENGDYPHPDHIRTHEISVAAFVAAGDPGRH
                     PDAGPPWQPLKLYYHASWSLHRLETIHAGAAARGIESPYGKWLETWEREREEGKAAEW
                     REPRITTQVDVGGWLERRRDALIAHATQIDPNGFWFAVPDQVLAEVYPWEDYTLVRSL
                     VPGAGASADADSGPVERDLFEGIDADGNPL"
     gene            3149..4561
                     /locus_tag="VF995_10315"
     CDS             3149..4561
                     /locus_tag="VF995_10315"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX9343994.1"
                     /translation="MPKQAQDQAAGQVHDSILDTVGGTPLVRLARLGRGLEPALLGKV
                     ESLNPGGSVKDRIGLDMIERAEADGRLHPGGTVVEPTSGNTGAGLAIVAALKGYRCVF
                     VMPDKVSQEKIALLRAYGAEVVVTPTAVAPDSPESYYAVANRLTEEIPGAFQPNQYAN
                     QANPAAHYRTTGPEIWEQTGGEIDMFVAGIGTGGTITGAGRYLKERNPAVQVVGADPE
                     GSIYSQPDDVHTYLVEGVGEDFYPETLDRTVVDRYLTVSDRDSFVMARRLAREEGILA
                     GGSGGMAAHAAVTLAKDLGPGTTIVVLLPDTGRGYLSKVFNDDWMREHGFLPHRRAAA
                     VADVLAGKVHHDTPPLVTVASHQRVSDAIDLLHSYSVSQLPVVSGDDPSDLTSVVGSV
                     QEGALLERLFRKPEVLDAQIVDVMEPPFPQVQEHQPVEEAFELLSKGRSTAVLVTRQG
                     RAAGVMTRSDILDYLATHRR"
     gene            4566..4715
                     /locus_tag="VF995_10320"
     CDS             4566..4715
                     /locus_tag="VF995_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9343995.1"
                     /translation="MPRTQPASDLAARLAPIELPEAGTGGAPRRLGTLWDQRPLLLVH
                     LRHFG"
BASE COUNT          710 a         1768 c         1660 g          627 t
ORIGIN      
        1 caggttggtg agcacctgca ggatcggctc ggcggcggcg tgcagcggca gggtgtcgtc
       61 gaccaggatc tcgatcgggc gggtcgggtg gcagacgcgc gcggcgctga ccgcctcgcg
      121 gaccacctga gcgccgtcgg aggctgggac ctggggctgg gtgccattcc cgtcgacgtc
      181 tcctggctgc cctgtggcct cggcctcgtg ggagctgagc aggagctggt cgaccaggag
      241 cttgaggcgg cgaccctgcc gggaggcgag gtcgaccagc tcctcccgtt cgtccgggcc
      301 gagctcgccg acgtgggcgc tgagggtgtc gagcgagccg agcaccaccg tgagggggtt
      361 gcgcaactcg tgggagacgg tggccagcag gtcgtgcttg aggcggtcct gctcctggag
      421 ctggcggacc agggcctgct tgccctggat catgcgggcc tgctcggcgg cgatggcggc
      481 gttggcctcg gcggcggctt cggcgcgggc gcgttccagc tcggcgcgct gggcgctgcg
      541 ggcggtgcgg tagagggcca ccatcggcag gccgagcagc gggatcagcg ccaggtagcg
      601 gtctgcgatg gccaccatca ccggggccag ggccagcagc agcgccgaag tccacagctg
      661 ctgcccgcgc ccgctaccgg aaccagcagc acgggcgccc gggccgcggg ccgccccggt
      721 gccgagggcg gaggcggagg cgggcagcag gttggcgagc agcaggaagg tggcggcggc
      781 ggtggcgaac gccggcagct cggtgaggct gggccccttg gggccgccga gcagccgcca
      841 gacgatgccg gcgccggcca gcgccaggat ccccttggcg gtggcgacca gcacccgcag
      901 gatcggcttg cggcccactg tgccggccag cagcgaggcg gcgccgaacg ccagcacggc
      961 ggcggcgatg ccccagccga gcagcagcgc gaaggcgaac ggctgcgcca tggtggccag
     1021 gtcgccgccc tcgccggcgg gcagctccag gggcatgtgc tcgccgacca ggatcagggc
     1081 ggcgaagagg aggaactggg cggggaagtt gccagccgcc cgcatccccg acggggacgc
     1141 gagcgcgaac agggtgaatc cgagcgccag aacagcgatc atggtgacgc gggcccagcg
     1201 tgtgagcccg cgacgggagg accccagccg ctcggatgag ggaagcaagc cgtgcagatg
     1261 caggctgcca aggcagccga tggagcgccg caccccctca ccccctctgg atgatcctgc
     1321 cagctcaact actcagtcac tatactcaaa cctgacgaag agtccaagca tagtgatcaa
     1381 gtcctacgtc aagaggctgt caagatcgcc gtcatcataa gcggtgcccg atgctgctcg
     1441 tcttgatcgt cgccataggc acactgcttg tgccactttt cggcggccgc ctgtcgctgc
     1501 tggcagacgc ccatgtccgc ctcgccggag tgctgccgct ggcgctcgcc ctgcaggtcc
     1561 tcgccgtcaa cgtgccgggt gtcccacagc acctccggcc tgtcctccag gtcgcctcct
     1621 atccggtcgg cctggtgttc gtcgccgcca actggcggct ggccgggatt cccctggtcg
     1681 gcctcggcgc cctcggcaac ctggcggcga tcctcgccaa cggcggcacc atgcccgcca
     1741 cggcggcagc actcgccagc gccggcctgg cggcccatcc gcaccgctac gccaactccg
     1801 ccgccgtcgc ccacccgcgc ctggccttcc tcggcgacct gttctcggtc ccgcgccggt
     1861 tcccgctgca caacgtcttc agcgtcggcg acctcctgat cgccctcggc gtcgtggtcg
     1921 ccgtccacgc cctctgcggc tcgcggccgc gcctccgcct tcgagggcag cgccagaagg
     1981 cgtgatcccg gcgcgttgtc ggcacgcttc gctacgcttg atggcgatcc attcgtgaag
     2041 catccgcccg tgctttagga gttgccgact cgtgtcacga atcctgctcg ccgtgcacgc
     2101 ccatcccgat gacgagtcgt cgaagggggc gggaacgctc gcccgctacg ccgaccaggg
     2161 cgtccggacg gtgatcgtca cctgtaccgg cggtgaggcc ggcgagatcc tcaacccggc
     2221 gatggacaag ccgggcaacc tggagcggat gaccgagcta cggcgcgagg agctggccaa
     2281 ggcactcgag atcctgaacg tcagcgccca ctacttcctc ggctaccggg actcgggcat
     2341 gcccgacacc gagaccaacg cccaccccga cgccttcgtc aacgccccgc tcgacgaagc
     2401 gatcggccgg ctggtgcgga tcatccgcga ggagcgcccg caggtgctgg tcacctacga
     2461 cgagaacggc gactacccgc atcccgacca catccgcacc cacgagatct cggtggccgc
     2521 gttcgtggcc gccggcgatc ccggccgcca tcccgacgcc ggcccgccgt ggcagccgct
     2581 gaagttgtac taccacgcca gctggtcgct gcaccggttg gagacgatcc acgccggcgc
     2641 ggccgcccgg gggatcgaga gcccctacgg caagtggctg gaaacgtggg agcgggagcg
     2701 cgaggagggc aaggcggccg agtggcgcga gccgcggatc accacgcagg tggacgtcgg
     2761 cggctggctg gagcggcgcc gcgacgcgct gatcgcccac gccacccaga tcgatccgaa
     2821 cggcttctgg ttcgccgtcc ccgaccaggt cctggccgag gtctacccgt gggaggacta
     2881 caccctggtc cgctcgctgg tgcccggcgc cggcgccagc gccgatgccg actcggggcc
     2941 ggtggagcgc gacctgttcg agggcatcga cgccgacggc aaccccctgt gacccacaac
     3001 cgcatcgaag ccgccaagat cgccgagcgc cacgctggct gtgacggccc ccggcgggtc
     3061 cggcctctac aattcccagg accaggggta ccgcacgatg ccgggcaacg gggccgcggc
     3121 cgcccccgat ccaaggaggc gcaccgcaat gcccaagcag gcccaggacc aggccgctgg
     3181 ccaggtccac gactcgatcc tcgacaccgt cggcggcacc ccgctggtgc gcctggcgcg
     3241 gctcgggcgc gggctggaac cggcgctgct cggaaaagtg gagtccctca accccggtgg
     3301 gtcggtgaag gaccgcatcg gcctggacat gatcgagcgg gccgaggccg acgggcggct
     3361 ccaccccggc ggcacggtgg tcgagccgac cagcggcaac accggcgcgg gcctggcgat
     3421 cgtcgccgcg ctcaagggct accgctgcgt cttcgtcatg cccgacaagg tcagccagga
     3481 gaagatcgcg ctgctgcgcg cctacggcgc cgaggtggtg gtcaccccga cggcggtcgc
     3541 gccggactcg ccggagtcgt actacgcggt cgccaaccgc ctcaccgagg agatccccgg
     3601 cgcgttccag cccaaccagt acgccaacca ggccaacccg gcggcgcact accgcaccac
     3661 cggtccggag atctgggagc agaccggcgg tgagatcgac atgttcgtcg ccgggatcgg
     3721 caccggcggc accatcaccg gcgccggccg ctacctcaag gaacgcaacc ccgcggtcca
     3781 ggtcgtcggc gccgacccgg aggggtcgat ctactcccag cccgacgacg tccacaccta
     3841 cctggtcgag ggcgtgggcg aggacttcta cccggagacc ctcgaccgga cggtggtgga
     3901 ccgctacctg accgtctccg accgggactc gttcgtgatg gcccgccgcc tcgcccgcga
     3961 ggagggcatc ctggccggcg gctccggcgg catggccgcc cacgcggccg tgaccctggc
     4021 caaggacctc ggccccggca cgaccatcgt ggtgctgctg cccgacaccg gccgcggcta
     4081 cctctccaag gtcttcaacg acgactggat gcgcgagcac ggcttcctgc cacaccgccg
     4141 cgccgccgcc gtcgccgacg tgctcgccgg caaggtccac cacgacaccc cgccgctggt
     4201 caccgtcgcc tcccaccagc gcgtcagcga cgcgatcgac ctgcttcaca gctactcggt
     4261 cagccagctc ccggtggtct ccggcgacga cccctcagac ctcaccagcg tggtcggctc
     4321 ggtccaggag ggcgccctgc tggagcgcct cttccgcaag cccgaagtgc tcgacgccca
     4381 gatcgtcgac gtgatggagc cccccttccc gcaggtccag gagcaccagc cggtcgagga
     4441 ggccttcgag ctgctctcca agggccgctc caccgccgtc ctggtcaccc gccagggccg
     4501 cgccgccggc gtgatgaccc gctccgacat cctcgactac ctcgccaccc accggaggtg
     4561 accggatgcc acgcacccag cccgccagcg acctcgccgc gcgcctcgcg ccgatcgagc
     4621 tgccggaagc cggaaccggg ggagcgccgc ggcgcctcgg gacgctgtgg gaccagcggc
     4681 cgctgctgct ggtccacctg cggcacttcg ggtgaatgtt ctgccgcgag cgggtcgcgc
     4741 agttgcgtga ccacgagggc gagat
//
LOCUS       DASWDC010000322         6964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_468867, whole genome shotgun sequence.
ACCESSION   DASWDC010000322 DASWDC010000000
VERSION     DASWDC010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468867"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..2128
                     /locus_tag="VF995_10325"
     CDS             371..2128
                     /locus_tag="VF995_10325"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX9343996.1"
                     /translation="MKHKRASRITWRVVSAEPTDRVEEVYCAVVPTTESFVLEDNLLT
                     GNCRSKMDGLVAATRLAATRQEEAALELVGWALDSETGSRLDAPPGTRDDAWDLLATS
                     GEDCPGQKGCGFFSRCFAEQARERARGVDVVVTNHHLLLLELELRELSDAAGVMLPES
                     DVIVIDEAHRLADHASDLYGISVTAARVTRAGAAADAAAKVAGQHGDWARRLRARWED
                     HASGLRPGPVLPGSRAAATLAAALQPVREEARAAAASLRELPASEELGGGIQAARRRL
                     TTLGRDLARLAESAEAGQGPGATVADLAIWVEERRDGRGIRSALVEPGARLEAVLWNP
                     VPATIACSATLAMAGSLDEAARGLGAGAPDALVADSPFDFQHNAMLYVPRELPLPSSD
                     RFRDAAEQELRALVEASSGRALILCTSWRALESSAAALAPLPYPLLVQGDDTPARLAD
                     RFRDEVASVLVATRTFFEGVDVPGESLSLLVLDKLPFPPPDDPLLAERGRRIQRRGGS
                     KFTSVWLPIAAVSLQQALGRLIRTESDRGVMAVLDRRLADAGYSMTLLASLPPAPLTR
                     SLDDVRRFFRDGEGEAPRE"
     gene            2185..2646
                     /locus_tag="VF995_10330"
     CDS             2185..2646
                     /locus_tag="VF995_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014913527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9343997.1"
                     /translation="MPTPEERLKELGIDLPQPVAPVAAYVPCVRTGNLVYVSGQVPQV
                     AGELPWKGRLGAEVSFVDGVAAARQCAVNVLAALKAELGELSRVRRVVRVGGFVASAA
                     DFTDQPRVINGASELFGEVFGEAGKHARAAVGVAALPLGVPVEVEALVEVE"
     gene            complement(2675..3184)
                     /locus_tag="VF995_10335"
     CDS             complement(2675..3184)
                     /locus_tag="VF995_10335"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HEX9343998.1"
                     /translation="MAIFKRIGGNRLRFRLSLEEVSLLHDLPEQLRELLGDERASQTG
                     EGADPVTRRLFPPAYLEPADAEQDAEFHRLMRDDLLRTKLDQLELVSKTLARGNVSSR
                     RWTVELSEEEAAAWLGVLNDVRLALGVRLGITEDFDVPVDLPGQPPELQLLAYLGWLQ
                     EHLVEALSA"
     gene            complement(3188..3481)
                     /gene="clpS"
                     /locus_tag="VF995_10340"
     CDS             complement(3188..3481)
                     /gene="clpS"
                     /locus_tag="VF995_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018565564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX9343999.1"
                     /translation="MASTAPTKERTEQPEDVAEPDVPWIVIVWNDPINLMSYVVFVFQ
                     KLFGYSKEKATKLMLDVHHKGKAVVSSGPRERAELDVYRLHSYGLWATMQKDR"
     gene            3566..4153
                     /locus_tag="VF995_10345"
     CDS             3566..4153
                     /locus_tag="VF995_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEX9344000.1"
                     /translation="MTTSDARDPIALLSRALDQAGVIIAKVSPDQAALPTPCRSWDVR
                     ALVNHVVQDVRMFTASAGGAAWQPGDEDLIGDDWTGTYQHAAEALLGAWQAPGILDRT
                     LRLPIGEVPASWSVHQQVTDLAVHAWDVAKATGQPTNLDPEIGQLSLDWAKRNLAPQF
                     RGDEASGKAFGDEVPVPDDAPLYDRLAGFFGRDPN"
     gene            complement(4176..6074)
                     /gene="selB"
                     /locus_tag="VF995_10350"
     CDS             complement(4176..6074)
                     /gene="selB"
                     /locus_tag="VF995_10350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX9344001.1"
                     /translation="MGHVIGTAGHVDHGKSALVLALTGTDPDRLAEEKARGMTIDLGF
                     AWTRLPSGREVGFVDVPGHERFIHNMLAGVGGVDCALFVVDATEGWMPQSAEHLAVLD
                     LLGIRAGVVAVTKADLCDPATVEAVAVQVVERLRGTTMEGAAIVPAAAPPGPGQGRQV
                     DALRAALDQALDRAAEPADRGRPRLPVDRVFSARGSGTVVTGTLSGGILTADVEAALL
                     PAGRRVRIRALQGHGAPLARAEPARRLAVNLAGVATGEVARGDVLVLPGQWAPTQVAD
                     CRLRCLAAASAPLRARGAYLLYAGAAETAVRLQPLDAREVVPGEEALVRLHFERPLVL
                     DFLEPVVLRDSGRDETVGGGRVLDPDPRGGVHGTAMRVRRAEELEAREGAGRAGLLGC
                     VLAERGVVPRADLPRLTGSGDLTRSIGSGAVQSRTLAWSARAWEAAQAAVLMAVERFH
                     RDQPLAAGLPAPAARRAALAAAAGAGAGWPAAAGAEVVEALVEAGRLLLDRAELRLPG
                     HGVRLDPAQEAVRAKVEAAASGGGVSVLTAAELAALGADRRLLVVLVRLGVLVALAPG
                     RYLGSATLDQAVRRLRDAFPAGRPFSASEAKEALGTTRKTAIPLLEYLDQHGVTARAG
                     DLRRVREP"
     gene            complement(6062..>6964)
                     /locus_tag="VF995_10355"
     CDS             complement(6062..>6964)
                     /locus_tag="VF995_10355"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX9344002.1"
                     /translation="LVEVGTTNRTHLHDYARALDTGEPTGCVLAVHPSNYRVEGFTTR
                     PGLDELAALAHEHGLPLLHDLGSGLLDGSFGAEPSVAGSLGAGADLVVFSGDKLLGGP
                     QAGLVVGRADLIGRLARHPVARAVRADKLTIAALEATLAAHVAGRRDELPVWRAMLLG
                     PEDLRPRAAALAAALGPAARLRDGASVAGGGSLPGEGLPSVLVEVQAGPGGEDAMLAR
                     LRAGDPPVIARAEHGRVVIDLRTVPPEQDELVATALRRALTPEPPDGSGGSGGTVPRP
                     EPPPADPTAAAGPPDGSGGDRWVT"
BASE COUNT          963 a         2617 c         2474 g          910 t
ORIGIN      
        1 gttcggtatc gcgcacggga tggtgtacgg ggacggcacc gcctttggcg caaccccagg
       61 agggtggagg accgcaacga tggaggaggc gccctcgtac ctccatggct ggcttgccgg
      121 ttattttgcg gcggacggat cagtccagaa gaagggaacc gcatctctct tctctgcaag
      181 tcgtgacaat cttgaatttg taaggactcc ctgttcccac ctggggatcg gcacctacgg
      241 catcggggag cgctaccgcg tagggatgcg gcagacagag gcaactcccc tctatgatat
      301 ggcctttgtg agctccacgt tacgccctga attctttgtg ataaaagagc atcggaagcg
      361 atttagcgac atgaagcaca agagggccag ccgcatcacc tggagggtcg tttctgccga
      421 gcccaccgac agagtcgaag aggtctactg tgcggtggtt cctaccaccg agtcgttcgt
      481 gcttgaggac aacctcctca ccggcaactg ccgcagcaag atggacggcc tggtcgccgc
      541 cacccgcctc gccgccaccc ggcaggagga ggcggccctc gagctggtcg gctgggcgct
      601 cgactccgag acgggctcgc ggctcgacgc cccgcccggc acccgggacg acgcctggga
      661 cctgctcgcc acctcgggcg aggactgccc cgggcagaag ggctgcggct tcttcagccg
      721 ctgcttcgcc gagcaggcca gggagcgggc acgcggggtg gacgtggtgg tcaccaacca
      781 tcacctgctg ctgctcgaac tggagctgcg ggagctctct gacgccgccg gggtgatgct
      841 gcccgagtcc gacgtgatcg tgatcgacga ggcgcaccgg ctcgccgacc acgcctccga
      901 cctgtacggg atcagcgtga ccgccgcgcg ggtcacccgc gccggcgccg ccgccgacgc
      961 cgccgccaag gtggcgggcc agcacggcga ctgggcgcgc cgcctccgcg cccgctggga
     1021 agatcatgcc agcggcctcc gccctggccc ggtcctcccc ggctcccggg ccgcggccac
     1081 cctcgcagcc gccctgcagc cagtgcggga ggaggcccgc gcggccgctg cctcgctgcg
     1141 ggagctgccc gccagcgaag agctgggggg tggcatccag gcggccaggc gacgcctcac
     1201 caccctcggt cgcgacctgg cccgcctggc cgaatccgcc gaggccggtc agggcccggg
     1261 ggccaccgtc gccgacctcg ccatctgggt cgaggagcgc cgcgacggcc gcgggatccg
     1321 ctcggcgctg gtcgaacccg gcgcgcggtt ggaggcggtc ctgtggaacc cggtgcccgc
     1381 cacgatcgcc tgctcggcga cgctggccat ggccggcagc ctggacgagg ccgcccgcgg
     1441 gctcggcgcc ggcgccccgg acgcgctggt ggccgattcg ccgttcgact tccagcacaa
     1501 cgccatgctc tacgtgccgc gcgagctgcc gctgcccagc agcgaccggt tccgtgacgc
     1561 cgccgaacag gagctgcgcg ccctggtcga ggcgtccagc ggacgcgcgc tgatcctctg
     1621 cacctcgtgg cgggcgctgg agtcgtctgc cgcggccctc gcccccctgc cctatccgct
     1681 gctggtgcag ggcgacgaca cccccgcccg cctcgccgac cgcttccgcg acgaggtggc
     1741 cagcgtcctg gtcgccaccc ggaccttctt cgagggtgtg gacgtgccgg gggagagcct
     1801 gtcactgctg gtgctggaca aattgccgtt cccaccgccc gacgacccgc tgctcgccga
     1861 gcgcggccgc cgcatccaac ggcgcggcgg ctccaagttc acctcggtat ggctgccgat
     1921 cgcggcggtc tcactgcagc aggccctcgg ccggctgatc cggaccgaga gcgaccgcgg
     1981 tgtcatggcg gtgctcgacc gccgcctggc ggacgccggc tacagcatga ccctgctcgc
     2041 gagcctgcca cccgcgccgc tgacccgctc cctcgacgac gtgcggcgtt tcttccgtga
     2101 cggagagggg gaagcgccgc gggagtagcg tatgaagcgc ctctgaacca aagatccgga
     2161 accgcacgcc agcgagggag cccaatgcca accccagagg agcgcctgaa ggaactcggc
     2221 atcgacctgc cgcagcctgt tgccccggtc gccgcgtacg tgccgtgcgt gcgcaccggc
     2281 aacctggtct acgtctccgg ccaggtgccg caggtagccg gcgagctgcc ctggaaaggc
     2341 cgcctcggtg cggaggtcag cttcgtcgac ggcgtggcgg ccgcgcggca gtgcgccgtc
     2401 aacgtcctcg cggcgctcaa ggcggagctg ggggagctgt cccgcgtccg ccgggtcgtc
     2461 cgcgtcggcg gcttcgtggc cagcgccgcc gacttcaccg accagccgag ggtgatcaac
     2521 ggcgccagcg agctgttcgg cgaggtcttc ggcgaggccg gcaagcacgc ccgcgccgcc
     2581 gtcggtgtgg cggccctgcc gctcggcgtc cctgtcgagg tggaggccct ggtcgaagtc
     2641 gagtagtggc gccgccggcc ggcgttcagg cgcctcaggc cgagagcgcc tcgaccaggt
     2701 gctcctggag ccagccgaga taggcgagga gctgcagctc gggcggctgg ccgggcaggt
     2761 ccacggggac gtcgaagtcc tcggtgatgc cgagccgcac cccgagggcg aggcggacgt
     2821 cgttcagcac ccccagccat gccgccgcct cctcctcgct cagctcgacc gtccagcgcc
     2881 gcgagctcac gttgccccgg gcgagggtct tgctgacgag ctcgagctgg tcgagcttgg
     2941 tgcgcagcag gtcgtcgcgc atcagccggt ggaactcggc gtcctgctcg gcgtcggccg
     3001 gctccaggta ggccggtggg aacaggcgcc gcgtcaccgg atcggcgcct tccccggtct
     3061 ggctggcccg ctcgtcgccg agcagctccc gcagctgctc tggcaggtca tggagcagcg
     3121 agacctcctc caggctgagg cggaaccgga gccggttgcc gccgatccgc ttgaagatcg
     3181 ccaccgccta gcggtccttc tgcatggtcg cccagaggcc gtagctgtgg agccggtaga
     3241 cgtccagctc ggcccgttcc cgcggcccgc tggagacgac cgccttgccc ttgtggtgga
     3301 cgtcgagcat cagtttggtc gccttctcct tggagtagcc gaacagcttc tggaacacga
     3361 acaccacata ggacatgagg ttgatcgggt cgttccagac gatgacgatc caggggacgt
     3421 ccggctcggc gacgtcctct ggctgctcgg tgcgttcttt ggttggtgct gtcgaggcca
     3481 tgctctatgg ttgcaaatct cgaaggccac tggaacttgc ctggctgaaa gcaatcgagc
     3541 cgaaacccct caggaggtcc accgcatgac gacgagcgat gcccgcgacc cgatcgccct
     3601 gctctcccgg gcactcgacc aggccggcgt catcatcgcc aaggttagcc ccgaccaggc
     3661 ggcgctgccg accccgtgcc gttcctggga cgtccgcgcc ctggtcaacc acgtggtgca
     3721 ggacgtgcgg atgttcaccg ccagcgcggg cggcgcggcg tggcagccag gcgacgagga
     3781 cctcatcggc gacgactgga ccggcaccta tcagcatgcg gccgaggcgc tgctcggggc
     3841 ctggcaggct ccggggattc tggaccggac gttgcggctg ccgatcgggg aggtcccggc
     3901 cagctggtcg gttcaccagc aggtcaccga cctggccgtc cacgcctggg acgtcgccaa
     3961 ggcgaccggc cagccgacca acctggaccc ggagatcggg cagctgtcgc tcgactgggc
     4021 caagcggaac ctcgcccccc agttccgcgg tgacgaggcc agcggcaaag ccttcggcga
     4081 cgaagtcccc gtccccgacg acgcgccgct ctacgaccgg ctcgcgggat tcttcggtcg
     4141 cgacccgaac tgagaggctg cctcgaaccg cgagcctacg gttctcggac ccggcgcagg
     4201 tcgccggctc gggcggtgac gccgtgctgg tcgaggtatt cgagcagcgg gatggcggtc
     4261 ttgcgggtgg tcccgagcgc ctccttcgcc tcgctggcgg agaacggccg ccccgccggg
     4321 aaggcgtcgc ggagccgccg gaccgcctgg tcgagcgtgg cgctgccgag gtagcgcccg
     4381 ggcgcgagcg ccaccagcac cccgaggcgc accagcacca cgagcagccg gcggtcggcg
     4441 ccgagcgcgg ccagctccgc cgcagtcagc acgctgacgc cgcccccgct ggcggctgcc
     4501 tcgaccttgg cgcggaccgc ctcctgggcc gggtcgagcc gcacgccatg gccggggagt
     4561 cgcagctccg cacggtccag cagcagccgc ccggcctcca ccagcgcctc caccacctcg
     4621 gcgccagccg ccgccggcca tccggcgccg gcgcccgcgg ccgctgccag cgcggcgcgc
     4681 cgcgccgccg gcgcgggcag gccggccgcg agcggctggt cgcgatgaaa ccgctccacc
     4741 gccatgagca ccgccgcctg cgccgcctcc cacgcccgcg ccgaccacgc cagggtgcgg
     4801 gactggacgg cgccgctccc aatcgatcgg gtgaggtctc cggagcccgt cagccggggg
     4861 aggtcggcac gcgggaccac cccacgctcg gccagcacgc agccgagcag accggcgcgg
     4921 ccggcgccct cccgagcctc cagctcctcc gcgcgccgca cccgcatggc ggtgccgtgg
     4981 acgccgcccc gagggtcggg gtcgaggacc cggccgccgc cgacggtctc gtcgcggccg
     5041 ctgtcacgca gcaccaccgg ttcgaggaag tcgagcacca gcggccgctc aaagtggagg
     5101 cggaccaggg cctcctcgcc cggcacgact tcgcgggcgt cgagcggctg caggcgcacg
     5161 gccgtctccg ccgccccggc gtagagcagg taggcgccgc gcgcccgcag cggagcggaa
     5221 gcggccgcca ggcagcgcag gcggcagtcc gccacctggg tgggcgccca ctggccgggc
     5281 agcaccagca cgtcgccgcg cgccacctcg ccggtggcga cgcccgccag gttgacggcc
     5341 agccggcggg ccggctccgc cctggccagc ggcgcgccgt ggccctgcag cgcgcggatg
     5401 cgcacccgcc ggccggccgg cagcagcgca gcctcaacgt cggcggtgag gatcccgcct
     5461 gacagcgtgc cggtcaccac cgtgccgctg ccccgcgctg agaagacccg gtccaccggc
     5521 agccgcggcc tgccccgatc agctggctcg gccgcccggt cgagcgcctg gtcgagcgcc
     5581 gcccgcaggg cgtcaacctg tcggccctgg ccagggccag gaggcgcggc ggcgggcacg
     5641 atcgccgcgc cctccatcgt ggtgccgcgc aaccgctcga cgacctgaac ggcgaccgcc
     5701 tcgacggtgg ccgggtcgca caggtcggcc ttggtgacgg cgaccacgcc ggcgcggatg
     5761 ccgagcaggt cgagcacggc gagatgttcg gcggactggg gcatccagcc ctcggtggcg
     5821 tcgaccacga acagcgcgca gtcgaccccg ccgaccccag ccagcatgtt gtggatgaag
     5881 cgctcatgac ccggtacgtc cacgaagccg acctcccgcc cggatggcag ccgggtccag
     5941 gcgaagccga ggtcgatggt cattccgcgt gccttctcct cggcgagccg gtcgggatcg
     6001 gtgccggtga gcgccaggac cagcgccgac ttgccgtggt cgacatgccc ggcggtcccg
     6061 atcacgtgac ccatcgatca cctcccgacc catcgggggg ccccgcggca gccgtgggat
     6121 cggctggcgg tggttccggc cgcgggaccg tgccccccga accccccgac ccatcggggg
     6181 gctccggcgt cagcgcccgg cgtaacgccg tggcgaccag ctcgtcctgc tctggaggga
     6241 cggtgcgcag gtcgatcacc acccgcccgt gctcagctcg ggcgatcacc ggtgggtcgc
     6301 cggcgcggag ccgagcgagc atggcgtcct caccaccggg accggcctgc acctcgacca
     6361 ggacgctggg caggccctca ccagggaggc tgccgccgcc ggccacgctg gccccgtcgc
     6421 ggagccgggc agccgggccg agcgccgccg ccagtgccgc cgcacgcggc cggaggtcct
     6481 cggggccgag cagcatggcc cgccacaccg gcagctcgtc ccgccgcccg gcgacgtggg
     6541 cggccagggt cgcctccagc gcggcgatgg tgagcttgtc ggcccgcacc gcccgcgcga
     6601 ccggatgacg ggccagccgc ccgatcaggt ccgcgcgccc caccaccagc ccggcctgcg
     6661 gcccgccaag cagcttgtcg ccggagaaca cgaccaggtc agctccggcc cccaggctgc
     6721 cggcaaccga cggctccgcc ccgaacgagc cgtccagcag cccagagcca aggtcgtgca
     6781 gcagcggcag cccgtgctca tgcgccagcg cggcgagttc gtccagtcct ggacgtgtgg
     6841 tgaaaccctc cacccggtag ttggagggat ggacagcgag gacacagcca gtcggctcac
     6901 cagtgtcgag cgcccgcgcg tagtcgtgca ggtgggtgcg gttggtggtg ccgacctcga
     6961 ccag
//
LOCUS       DASWDC010000323         5026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_645464, whole genome shotgun sequence.
ACCESSION   DASWDC010000323 DASWDC010000000
VERSION     DASWDC010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645464"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1010)
                     /locus_tag="VF995_10360"
     CDS             complement(144..1010)
                     /locus_tag="VF995_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017603441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX9344003.1"
                     /translation="MTARVLDGKAVAAQIRAEAMEEVEKLAALGRRPGLAAVLVGDDE
                     ASHIYVGAKQRTAERWGIASRRVSLPASATEDDVLAAVGELNADDVVHGIIVQLPLPA
                     GLDPVRVQEAIDPAKDVDGLHPWNEGRVLRGDPGLAPCTAVGIVELLRREHVPVEGSH
                     VVIVGRGLLVGRPLAVLLSAKAPGANATVTLCHTGTRDVVQFTRAADVLVAAAGIPRM
                     ITPEMVKPGAAVVDVGNHRVGGRLVGDVVPEVAEVAGALTPVPGGVGPMTVAMLMANT
                     VTAAHDLAGPGG"
     gene            complement(1130..2740)
                     /gene="purH"
                     /locus_tag="VF995_10365"
     CDS             complement(1130..2740)
                     /gene="purH"
                     /locus_tag="VF995_10365"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX9344004.1"
                     /translation="MNDRDRAHRQPLRRALVSVFDKAGLERLAAALSGARIEVVSTGS
                     TAAALEGHGLDVTRVEQLTGFPEMLDGRVKTLHPRVHAGLLADRRQPGHVEQLEAAGI
                     APFDLLVVNLYPFEETIAELDPSGDPAATEGLAIERIDVGGPAMLRAASKNFASLAVI
                     CDPADYGLVEAALAAGGTTLEERRALAAKAFARTAAYDTAVAGWFAATAPASGPQDAD
                     QDGGFPERLVLTGRLRQRLRYGENPHQAAAFYGVEGGPVWGLAAAAQLAGKELSYNNL
                     LDADAVLALVAEHADRPFAAIVKHTNPCGAAFADRLEDAYAAALDGDPVSAFGGVVGL
                     SRPLDAATATRIAGIFTEVVLAPAFEEEALVVLSGKPSLRLLQVDLARPASARQVRTV
                     AGGLLAQEADRTADDPATWKQLAGDPPAEAALEDLALAWSVAGHVKSNAIVLARDGRI
                     VGVGAGQQSRVDSVRIAVAKAAGRAHGAVAGSDAFFPFPDGVEALAAAGVRAIAHPGG
                     SVRDDEVTAAAAAAGVAMFHTGRRHFRH"
     gene            complement(2737..3363)
                     /gene="purN"
                     /locus_tag="VF995_10370"
     CDS             complement(2737..3363)
                     /gene="purN"
                     /locus_tag="VF995_10370"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX9344005.1"
                     /translation="MKRVVVLISGDGSNLQALIDRAADPKFGAAVVLVVSDRAEAGGL
                     ERARRVGIATAVVRLADHPDRGAFDRAVRDVVAAARPDVVCLAGFMRILGPEFIRAFP
                     NRIVNTHPSLLPAFRGAHAVREALAYGVRLSGCTIHLVDEQVDHGPVLLQAAVPIEDG
                     DDEASLHQRIKREEHQLLPRAVQLLAHGRVRVNGRRTTIVEGSGGETA"
     gene            3593..4009
                     /locus_tag="VF995_10375"
     CDS             3593..4009
                     /locus_tag="VF995_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014689037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEX9344006.1"
                     /translation="MTRVGAKRSRSLPEQLGFVGLGAAGASFAYPAVHQATGLGVFCP
                     LRTLTGMPCPACGMTTAATRLAAGDLHGALAANPFVLVLAGLTLFMAVLLALRGGGLA
                     PPPVAWSVGRQRAAKAAVVMLAAGSWLFQLHRFGWI"
     gene            4108..4776
                     /locus_tag="VF995_10380"
     CDS             4108..4776
                     /locus_tag="VF995_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344007.1"
                     /translation="MSEGTSPRDPRERPPPPPQPPPGWGPPGPGSGQGWGPPPPQQGW
                     GQQQNWGGQQGWGQQPPGWGQQPQDGSGGFSENVAGGLTYLVAFVILLLSGLISFPTR
                     IILAMVPCGVGLALVLTDRRREVRFNALQGTLLWVIMVVAEIGRHTAASLWTLPIIVG
                     SAAAEIALFVVLLGLMAAAFQGRHLKLPAIGDFAERQASGHGGGHSPRQGPGPGPQGW
                     GDPR"
     gene            4773..>5026
                     /locus_tag="VF995_10385"
     CDS             4773..>5026
                     /locus_tag="VF995_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344008.1"
                     /translation="MSQRQDQSQDGSRNGSHANNAAPPPPPPASYGQPPPPHADTGFD
                     VVDQPGTFMGRQLASWAERFGASLIDALLIVSPALAAMIY"
BASE COUNT          676 a         1878 c         1801 g          671 t
ORIGIN      
        1 cacgttgacc gcgtcgacca gcccgcgcag ccgccgcgcg atgcgccgca ccggctccgg
       61 gtccggcgac cggggcggga ccagctcgcc ggtgaccacg aagcacccct ggtccaggag
      121 tgccccgagc ctgccgagac cggtcaaccg cccggccccg ccaggtcgtg ggcggcggtg
      181 accgtgttgg ccatgagcat cgccacggtc atcggcccca cgccgccggg cacgggggtg
      241 agcgcgccgg ccacctcggc gacctccggg acgacgtcgc cgaccagccg cccgccaacg
      301 cggtggttgc ccacgtcgac cacggccgcg cccggcttga ccatctccgg ggtgatcatg
      361 cgcgggatcc cggcggcggc caccagcacg tcggcggcac gagtgaactg gacgacgtca
      421 cgggtgccgg tgtggcacag ggtcacggtg gcgttggccc ccggagcctt ggccgacagc
      481 agcaccgcca gcggccggcc gaccagcagg ccccgcccga cgatcaccac gtggctgccc
      541 tcgaccggta catgctcgcg gcgcagcagc tcgacgatcc cgacggcggt gcacggcgcc
      601 aggcccgggt cgccgcgcag cacccggcct tcgttccagg gatgcaggcc gtcgacgtcc
      661 ttggccgggt cgatcgcctc ctggacccga accggatcaa gcccggcggg caggggaagc
      721 tgcacgatga tcccgtggac gacgtcgtcg gcgttgagtt cgcccaccgc cgcgagcacg
      781 tcgtcctcgg tggcgctcgc cggcaggctc acccgccgcg aggcgatccc ccaccgctca
      841 gccgtccgct gcttggcgcc gacgtagatg tgcgacgcct cgtcgtcccc gaccagcacc
      901 gccgccaggc cgggccgccg ccccagcgcg gccagcttct cgacctcctc catcgcctcg
      961 gccctgatct gggccgcaac cgctttgccg tccagcactc tcgccgtcat gccaccctct
     1021 ccgctcgacg cccacgggcc catagagtag agccgatctc cggctcgccg gccacaaccg
     1081 ggcaaccatg cacggccgct caaccgtctg gcaggcccgg cccgttggtc taatggcgga
     1141 agtggcggcg gccggtgtgg aacatggcga cgccggccgc tgccgcggcg gcggtgacct
     1201 cgtcgtcgcg gacgctgccg ccggggtggg cgatggcgcg gaccccggcg gcggcgagtg
     1261 cctcgacgcc gtcggggaag ggaaagaagg cgtcgctgcc ggcgacggcg ccgtgtgctc
     1321 ggccggcggc cttggcgact gcgatgcgga ccgagtcgac gcggctctgc tggcccgcgc
     1381 cgacgccgac gatacggccg tcgcgggcca gcacgatcgc gttggacttc acatgccccg
     1441 cgaccgacca ggcgagcgcg aggtcctcca aggcggcctc ggcaggcggg tcgcccgcga
     1501 gctgcttcca ggtggcgggg tcgtcggcgg tccggtcggc ctcctgcgcc agcagtcccc
     1561 cggcgaccgt gcggacctga cgggcgctgg ccggcctggc cagatcgacc tggagcagcc
     1621 gcagggacgg cttgccggac agcaccacca gcgcctcctc ctcgaaggcg ggggcgagca
     1681 cgacctcggt gaagatcccg gcgatgcggg tggcggtggc ggcgtccaag ggccgcgaca
     1741 ggcccaccac cccgccgaag gcgctcaccg ggtcgccgtc gagcgcggcg gcgtaggcgt
     1801 cctccagccg gtccgcgaac gccgcgccgc acgggttggt gtgcttgacg atcgcggcga
     1861 acggccggtc ggcgtgctcg gcgaccagcg ccaggaccgc gtcagcgtcg agcaggttgt
     1921 tgtacgacag ctccttgccg gcgagctgcg ccgccgcggc gagcccccac acgggcccgc
     1981 cctccacacc gtagaaggcg gcggcctgat gcgggttctc gccgtagcgg agccgctgcc
     2041 ggagccgccc ggtgaggacg agccgctcgg ggaagccgcc gtcctggtcc gcgtcctgag
     2101 ggccggaggc cggggccgtg gcggcgaacc agccggcgac cgccgtgtcg taggcggcgg
     2161 tgcgggcgaa cgccttggcg gcgagggcgc ggcgctcctc cagggtcgtc ccgccggcgg
     2221 ccagggcggc ctcgaccagc ccgtagtcgg ccgggtcgca gatcaccgcc agcgaggcaa
     2281 agttcttgga ggcggcccgg agcatcgccg ggccgccgac gtcgatccgc tcgatggcga
     2341 ggccctcggt ggccgcgggg tcgcccgatg ggtcgagctc ggcgattgtc tcctcgaacg
     2401 ggtacaggtt caccaccagc agatcgaacg gggcgatccc ggccgcttcg agctgttcca
     2461 cgtgcccagg ctgccgccgg tcggccagca atccggcgtg gacgcggggg tgcagggtct
     2521 tgacccgccc gtcgagcatc tccgggaagc cagtgagctg ctcgacccgg gtcacgtcga
     2581 ggccatggcc ctccagggcc gccgccgtcg agccggtcga gaccacctcg atcctcgcgc
     2641 ccgagagcgc ggcggccagc cgctccagcc cggccttgtc gaacaccgag accagcgccc
     2701 ggcgcagcgg ctgacggtgg gcccgatccc tgtcgttcac gctgtctctc ctccgcttcc
     2761 ttcgacgatg gtggtccgcc ggccgttcac ccggacccgg ccatgggcga gcagctggac
     2821 cgcgcgcggc agcagctgat gctcctcccg cttgatgcgc tggtgcaggc tggcctcgtc
     2881 gtcgccgtcc tcgatcggga ccgccgcctg cagcagcacc gggccgtggt cgacctgctc
     2941 gtccaccagg tggatggtgc agccgctcag ccgcaccccg taggcgagcg cctcccgcac
     3001 cgcgtgcgcg ccccggaacg ccggcagcag gctcggatgg gtgttcacga tccggttcgg
     3061 gaacgcccgg atgaactctg gtcccaggat ccgcatgaac cccgccaggc agaccacgtc
     3121 cgggcgggca gccgccacca cgtccctgac cgcccggtcg aacgcccccc ggtcaggatg
     3181 gtcggccagc cgcaccaccg ccgtggcgat ccccacccgc cgggcacgct ccaagccccc
     3241 cgcctccgcc cggtcgctga cgaccaggac aacggcggca ccgaacttgg gatcggccgc
     3301 ccggtcgatc agcgcttgga gattggagcc gtcgccggag atcagcacga cgactcgctt
     3361 caccgagctc ctcctcgcgt ccttgcgtcc ttgcgccgcg ctcccggcac ggcgggagcc
     3421 gatggtacag gtgtgccaga tgggcgcgaa tctgctggca caagcgccct gaaggggaag
     3481 atgccgactg gcttcctgtg ccggtcaggc gggtaagccg tagactcggg cggtattggt
     3541 atccgcactg tggagcgtac gaaaggctga ggtggacacc gctcgaaccg tcgtgacgag
     3601 ggtgggagcc aagcggtcgc gctcgctgcc ggagcagctc gggttcgtcg ggctaggcgc
     3661 cgccggggcg tcgttcgcct acccggcggt gcaccaggcg accggcctgg gcgtgttctg
     3721 cccactgcgg accctcaccg ggatgccctg cccggcgtgc gggatgacca ccgcggcgac
     3781 ccgcctcgcg gccggagacc tgcacggcgc gctggccgcc aacccgttcg tcctggtcct
     3841 ggcaggcctg acgttgttca tggcggtgct gctggccttg cgcggcgggg gcctggcgcc
     3901 gcccccggtg gcatggtctg tagggaggca gcgcgccgcg aaggccgccg tggtcatgtt
     3961 ggccgccggg agctggctgt tccagctgca ccgcttcggc tggatctgag ccggcgcggc
     4021 ccggcggccc tcgtccggca ggcgaccagc aagcagtgag caagcatgag caggcgatga
     4081 gcgaagattg gcgatcagga ggagtcgatg tctgagggaa cctcgccgcg ggacccgcgt
     4141 gagcgcccgc cgcctccgcc acagccgcct ccaggctggg gaccgccagg gccaggatcc
     4201 ggccagggat ggggcccacc accaccccag cagggctggg ggcagcagca gaactggggc
     4261 ggccagcagg gctgggggca gcagccaccg ggctggggcc agcagccaca ggatgggtcg
     4321 ggcggcttct ccgagaacgt cgccggcggg ctcacctacc tggtggcgtt cgtgatcctg
     4381 ctgctctccg gcctgatctc gttcccaacc cggatcatcc ttgccatggt gccctgcggg
     4441 gtgggcttgg cgctggtctt gaccgatcgc cgccgggagg tccgcttcaa cgccctccag
     4501 gggaccctgc tctgggtgat catggtcgtg gcagagatcg gccgccacac cgcggcgagc
     4561 ctctggaccc tgcccatcat cgtggggtcc gccgccgcag agatcgccct cttcgtggtg
     4621 ctacttgggc tcatggccgc cgctttccag ggccgtcacc tgaagttgcc cgcaatcggc
     4681 gatttcgccg agcggcaggc gagcggccat ggtggaggtc acagcccacg ccagggtccc
     4741 ggaccgggac cgcagggctg gggtgaccca cgatgagcca gcgtcaggac cagagccagg
     4801 acggctcacg caacgggtcc cacgccaaca acgcggcccc cccacctccg ccgccagcca
     4861 gctacgggca gcctccgccc ccccacgccg acaccggctt cgacgtcgtc gaccagccag
     4921 gcaccttcat gggacgccaa ctggcaagct gggctgaacg cttcggcgcc tcgctgatcg
     4981 acgcgctgct gatcgtctcg ccggcgctgg ccgcgatgat ctattt
//
LOCUS       DASWDC010000324        10011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1138973, whole genome shotgun sequence.
ACCESSION   DASWDC010000324 DASWDC010000000
VERSION     DASWDC010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10011
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138973"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VF995_10390"
     CDS             complement(<1..897)
                     /locus_tag="VF995_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9344009.1"
                     /translation="MADRTPTLPPGAAAGAAMPDREFTVEARTQWQLVLRRFLAHKMA
                     VASLVIFLLLVFTSLFDGHFWKYKFDSPATSPPSHGPWLHNPLGTDDIGRDELALVLR
                     GAQRSMQIALLVTLLSTTFGATLGAVAGYYRGVMDSALMRFTDLVLTVPLFAVLAVLA
                     HIATGGSWLVIPFVLAALQWTTIARVVRGSFLSLREKEFVEAARALGASDRRIIFRHL
                     LPNTTGPIIVNATVIVAIAILLETALSYVGLGVHSPDTSLGLLVQTGQSAAQTRPWLF
                     YFPGLMIIIIALTINFIGDGLRD"
     gene            complement(890..1870)
                     /locus_tag="VF995_10395"
     CDS             complement(890..1870)
                     /locus_tag="VF995_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9344010.1"
                     /translation="MVSFVIRRLLISIPVVLLSSVLVFLLVANSGDPLSELKSRQPPV
                     PQEVIKAREHALYLDRSEPVRYGIWISHFVRGDMGTTLDGRDVRTLLGQRLPVTLRMI
                     SLAMLLALILGVGVGVVSAVKQYTPTDYTFTFFGFLFLSMPVFWLAALLKEFLAIRVN
                     QALGRVVFFTVGDQTPNLAGNLVQRLGNYAGHLVLPTVTLALITFASWSRYQRGSMLE
                     VLSSDYIRMARAKGLSRSRVMLRHALRNALIPLVTVAAIDIGALFGGAVITENVFSWH
                     GMGELLINGVTIGDVNVVLAWLMVTAIIVILFNLLADVLYAVLDPRIRYG"
     gene            complement(1988..3652)
                     /locus_tag="VF995_10400"
     CDS             complement(1988..3652)
                     /locus_tag="VF995_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HEX9344011.1"
                     /translation="MHGRRSSRWLLPLLGLALIAAACSSNKGNGGGGTSGGGIKEGGS
                     LNYAADQEPTGFNNNTSKDHGTSVLNITVNMFPNAFNVAPDLTVHLNSDLLASADLTK
                     QSPETIVYKIKPNASWSDGTPFSADDFIYAWQHQNGTNPKFDVADTTGYANIKSVTGS
                     DNGKTVTVVYSKPFADWKNVFTQMLPAHYMKSLPGSDVQQWNDGIDKNPEKIPSAGPF
                     VVQSYTVGNSLTLKRNDQYFGQKAHLGQIVFRFLPASTAQPQALQNSEVDLIYPQPQI
                     DEVKQVQALPDVTSQINFGLSFEHLDFNFKNQFLGDLKVRQAIATGLNREDLVNRTVK
                     QFSDKASVLGNRIWLSGQQYYEDHSGNYGKGDVNAAKALLQGDGYTMGSDGYFAKGGK
                     RLEVRFSTTAGNQLREQQGVLFQSQMKAIGIKVDIANVVSKTFFGDWLPKGNYDVADF
                     AWVGNPFAISNNHDIYRTGGGGNYGKLSDPKIDSLFNQAIAELDPAKSAALGNQIDQQ
                     IWNDMATIPLYQKPTFIAWRNTFTNIVDNTSQYGPFTQATSWAQKA"
     gene            complement(3814..4581)
                     /locus_tag="VF995_10405"
     CDS             complement(3814..4581)
                     /locus_tag="VF995_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEX9344012.1"
                     /translation="MAAPPPVPLPLPLPEDDGRVRALLEEGQLEVLGRIPWASNATML
                     AKICHDGLESLAVYKPARGERPLWDFPEGTLYRREVAAYLVSEQLGWGLVPLTVARDD
                     GPFGVGSLQRFVDADLEVTAFELVERGDATMPLIAAFDVVINNADRKAGHCLDDRSQG
                     SGGGRVWAIDHGVCFHVEPKLRTVLWDFAGQRLARRLLDDLRGLVAALEGELGERLTG
                     ILAPAEIEALTERTEQLTRSGRFPHPVGRRPYPWPLV"
     gene            complement(4594..5184)
                     /locus_tag="VF995_10410"
     CDS             complement(4594..5184)
                     /locus_tag="VF995_10410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEX9344013.1"
                     /translation="MASRDLDPVTRLTADAVGEPGQRTFLIQAASGPEQVTLLVEKEQ
                     VRVLAERLIAWLPELAADRPEDPEEVRAAEASDLSLAEPFEPDFRVGQLAITYDPDRD
                     RILVVATELLPDTGDEQEAALELDLELEGELEAALEREPREVRLWVTRPQLRVLARHG
                     SQVVERGRRPCPLCGNPLDPTGHICPAQNGHRPTLA"
     gene            complement(5171..5857)
                     /locus_tag="VF995_10415"
     CDS             complement(5171..5857)
                     /locus_tag="VF995_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HEX9344014.1"
                     /translation="MDRLLLLRHAVTEETGKRLSGWLPGLHLSEQGRQQAKTLAERLA
                     PLKIDALYASPLERCQETAAAVASVKGLEVTVAPDLGEVRYGDWTGRALRDLAKLPLW
                     RVVQGNPSAMRFPDGELPGESLYEMQTRAVAALERLRAAHPRQTVAVCSHADVIKAVA
                     AHYLGMHLDQYQRLVVAPASLTAFGFAPLPYLLRLNDVGLDDTALEVPPQPPQPEQLR
                     LLEDPVDGQP"
     gene            complement(5886..6692)
                     /locus_tag="VF995_10420"
     CDS             complement(5886..6692)
                     /locus_tag="VF995_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9344015.1"
                     /translation="MDLGLEGKRVLCTGGSGGIGQGICRALAAEGALVAVHHHRNEQA
                     AKRLAAELPGPGPGPGPSAVAVGGDLGTVEGAEAAVSAASDAFGGLDILVHCAGVWPP
                     EPLPLWRLSPERIEATLRVDLTAAFLAAGAFLRQLETQRQEHGNLVFIGSTAGIFGEA
                     DHADYAAAKSAVIFGLVRSLKNEIVRIAPRGRVNAVCPGWVRTPMTRAALEDPTLVPR
                     VTRTMPLRKLATVTDVATQVVLLASDTASGHVSGQIVTVAGGMEGRILHE"
     gene            complement(6696..7295)
                     /locus_tag="VF995_10425"
     CDS             complement(6696..7295)
                     /locus_tag="VF995_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9344016.1"
                     /translation="MLARADELTGVTASSPEDRAWVEGLPLRVHLNAALLEAEGDLAA
                     ATEGYLRAAGLEGYRIPHLRSDCHLGAARCLLALGRHAEAREHARQASRLLERWPGWR
                     RDETRALLRRLGDGQPVAGPDVLTAREREVAALLVEGLSNGEMARRLFISTKTASVHV
                     SNILAKLGMTSRAEIAAWASRNGLGTPPEPAPAAAPTGG"
     gene            complement(7271..9652)
                     /locus_tag="VF995_10430"
     CDS             complement(7271..9652)
                     /locus_tag="VF995_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9344017.1"
                     /translation="MLAGRNGLSPVMVGRASELARLRRLLAARSVPDARVALISGEAG
                     IGKTRLLKELIAGLDPDPDALVLGGQAGQGMPGRPFQLLREAVASEVRDWEAVPARLA
                     DRDDALRMMLYPIAPRLACDRERDREYGQEELLGTAVDLIRELAPTLLVFEDLHWADA
                     QSVALFGRLALTADLDVLLIGTFRPEGVDRRHPMLELLAELDRQRPVTSILLERLDQA
                     QVHELVEAVHGGPVPFRVAETLHRRTGGNPFFLEELLATAAKDLKGSDATAAPERLAS
                     LPLPWNLTEAVLRRLDDLEPPERQVAETAAILGERIPFDLLASVAGRSEQELIEALRR
                     LVREDLLAEREQDVFHFRHALTREAIAGQLLGRERRRLHEKALAALQEAGSDDWSAIV
                     WHAQGAGRGEELVAAARAGAEAYVCRGASVDALRLAEHGLAEAPDDPALLAWASKAAW
                     LIGLLDVSVEHGERRLEVARQRGDLEDQAAVLIHLARVHWEAHHPDRQWECVWQALEA
                     ARRLGPSPLLARAQARVSEAYMLADDATAAIEWADHTIALAERVGPRSVLLAAQVNKG
                     TAMVEPNGPPSRRRREEGLALLERAVAEAEAADFDLTLHRALWNLMVNQVQSWPLERS
                     WQVYRQLRAASERAGHAADNVGLAIRRSEIHVMEGDRHAALTAIAKGRRLTRTRNPSS
                     VFEFLLDPYEAVLRIEGGELPAAEELLGRSREMMLTASQECDRVWFWAICASLAARRG
                     DLDAARAACAQADAQVAAWWWGLGPEPNIGLIDAVAPVCQRRRCGRCSPEPTS"
     gene            complement(9707..9937)
                     /locus_tag="VF995_10435"
     CDS             complement(9707..9937)
                     /locus_tag="VF995_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344018.1"
                     /translation="MMQYHPELTRVLAEERRRGLFDDARARRLARNQKAARKGARRFL
                     SLRSRAAPSPAPSPGASAGAGAGRLSPRAKSA"
BASE COUNT         1394 a         3430 c         3701 g         1486 t
ORIGIN      
        1 gtcgcgcagg ccgtcgccga tgaagttgat cgtcagcgcg atgatgatga tcatcaggcc
       61 ggggaagtag aacaaccacg gccgggtctg cgccgccgac tgccccgtct ggaccagcag
      121 cccaagcgag gtgtccggcg agtggacccc caggccgacg taggagaggg cggtctccag
      181 caggatcgcg atcgccacga tgacggtcgc gttgacgatg atcggcccgg tggtgttggg
      241 cagcaggtgg cggaagatga tccgccggtc gctggcgccg agcgcgcggg ccgcctcgac
      301 gaactccttc tcgcgcagcg acaggaagct gccgcggacc acccgggcga tggtggtcca
      361 ctgcaacgcc gccagcacga acgggatcac cagccagctg ccgccggtgg cgatgtgcgc
      421 caggaccgcg aggacggcga acagcgggac ggtcagcacc aggtcggtga agcgcatcag
      481 cgccgagtcc atgacaccgc ggtagtagcc ggcgaccgcc ccgagggtgg ccccgaaggt
      541 ggtcgacagc agcgtcacca gcaacgcgat ctgcatcgag cgctgcgccc cgcgcagcac
      601 cagcgccagc tcgtcccggc cgatgtcgtc ggtgccgagc gggttgtgca gccacggccc
      661 gtggctcggc ggcgaggtcg ccggggagtc gaacttgtac ttccagaaat ggccgtcgaa
      721 cagggaggtg aacaccagca gcaggaagat caccaggctg gccacggcca tcttgtgggc
      781 gaggaagcgc cgcaggacca gctgccactg ggtccgcgcc tcgaccgtga attcccggtc
      841 gggcatcgcc gccccggcgg cggcgcccgg gggcagggtc ggcgtgcggt cagccatagc
      901 ggatcctcgg gtcgagcacg gcgtagagca cgtccgcgag cagattgaac aggatcacga
      961 tgatggcggt caccatcagc caggccagca cgacgttcac gtcgccgatg gtgaccccgt
     1021 tgatcagcag ctcgcccatg ccgtgccagg agaagacgtt ctcggtgatg accgcgccgc
     1081 cgaagagcgc gccgatgtcg atcgcggcca cggtgaccag cgggatcagg gcgttgcgca
     1141 gcgcgtggcg gagcatcacc cgcgagcgcg acagcccctt cgccctggcc atccggatgt
     1201 agtcgctcga cagcacctcg agcatgctgc cgcgctggta gcggctccag gacgcgaagg
     1261 tgatcagcgc cagcgtgacc gtcggcagga ccaggtgccc ggcgtagttg ccgagccgct
     1321 gcacgaggtt gccggccagg ttgggagtct ggtcgccgac ggtgaagaac accaccctgc
     1381 cgagcgcctg gttgaccctg atcgccagga actccttgag cagggcggcg agccagaaca
     1441 ccggcatcga caggaacagg aagccgaaga aggtgaaggt gtagtcggtc ggcgtgtact
     1501 gcttgacggc gctgaccacg ccgacgccga cgccaaggat cagggcgagg agcatcgcca
     1561 gcgagatcat ccgcagggtg accgggaggc gctggccgag cagggtgcga acgtcgcggc
     1621 cgtcgagggt ggtgcccatg tcgccgcgga cgaaatggga gatccagatc ccgtagcgga
     1681 ccggttccga gcggtccagg tagagcgcgt gctcgcgcgc cttgatgacc tcctggggca
     1741 ccggcggctg gcggctcttc agctcggaga gcgggtcgcc ggagttggcc accagcagga
     1801 acaccagcac tgaggagagc agcaccaccg gtatggagat gagcagccgg cggatgacga
     1861 agctgaccat ggcatccctc ttgccgtgcg gaccgcctgt gcgttgccgt tcccgtcctg
     1921 gtcccagttc ccgtggcgcg ggccggtggc gatccggccc gcgccatcag ggtctcaggg
     1981 tgaaggccta cgccttctgt gcccagctgg tggcctgggt gaacggcccg tactggctgg
     2041 tgttgtccac gatgttggtg aaggtgttgc gccaggcgat gaaggtcggc ttctggtaca
     2101 gcgggatggt cgccatgtcg ttccagatct gctggtcgat ctggttgccg agcgccgccg
     2161 acttggccgg gtccagctcg gcgatggcct ggttgaacag cgagtcgatc ttggggtccg
     2221 acagcttgcc gtagttgccg ccgccgccgg tccggtagat gtcgtggttg ttggagatgg
     2281 cgaagggatt gccgacccag gcgaagtcgg caacgtcgta gttgcccttg ggcagccagt
     2341 cgccgaagaa ggtcttggag accacgttgg cgatgtcgac cttgatgccg atggccttca
     2401 tctgcgactg gaacagcacg ccctgctgct cgcggagctg gttgccggcg gtggtggaga
     2461 agcggacctc cagccgcttg ccacccttgg cgaagtagcc gtcggagccc atggtgtagc
     2521 cgtcgccctg cagcagcgcc ttggcggcgt tgacgtcacc cttgccgtag ttgccggaat
     2581 ggtcctcgta gtactgctgc ccgctcagcc agatgcggtt gcccagcacc gaggccttgt
     2641 cgctgaactg cttcaccgtg cggttgacca ggtcctcgcg gttgaggccg gtggcgatcg
     2701 cctggcggac cttcaggtca cccaggaact ggttcttgaa gttgaagtcc aggtgctcga
     2761 acgacagccc gaagttgatc tgcgaggtga cgtcgggcag cgcctggacc tgcttgacct
     2821 cgtcgatctg cggctgcggg tagatcaggt cgacctcgct gttctgcagc gcctgcggct
     2881 gggcggtcga cgccggcagg aagcggaaca cgatctggcc gaggtgcgcc ttctgcccga
     2941 aatactggtc gttgcgcttc agggtgaggc tgttgccgac ggtgtagctc tgcaccacga
     3001 acgggccggc ggaggggatc ttctccgggt tcttgtcgat cccgtcgttc cactgctgca
     3061 cgtcggagcc gggcagcgac ttcatgtagt gggccggcag catctgggtg aagacgttct
     3121 tccagtcggc gaacggcttg gagtagacca cggtgacggt cttgccgttg tcggagccgg
     3181 tgacgctctt gatgttggcg tagccggtgg tgtcggccac gtcgaacttc gggttggtgc
     3241 cgttctggtg ctgccacgcg tagatgaagt cgtcggcgct gaacggggtg ccgtccgacc
     3301 aggaggcgtt cggcttgatc ttgtagacga tcgtctcggg gctctgcttg gtcaggtccg
     3361 ccgatgcgag caggtcgctg ttgaggtgca cggtgaggtc cggcgccacg ttgaaggcgt
     3421 tggggaacat gttcacggtg atgttcagca ccgaggtgcc gtggtccttg gaggtgttgt
     3481 tgttgaagcc cgtgggctcc tggtcggcgg cgtagttgag gctgccgccc tccttgatgc
     3541 ccccgccgct ggtgccgccg cctccgttgc ccttgttgct ggagcacgcc gccgcgatca
     3601 gcgcgagccc gagcaggggc agcagccacc tgcttgatcg tcgaccatgc attttatgcg
     3661 acctcctcgg ctggaatgcc gttgcccacc tgacggaatt gtggtggtcg taacgctggg
     3721 gggttgttcc cggcctacaa acgtttgggc gacgatgccg ccaatcggcc gcatcgtacg
     3781 acaagccttc tacctgcgtt cataagaatt tcctcagacc agtggccacg gatacggccg
     3841 gcggcccacc gggtggggga acctgccact gcgggtgagc tgctcggtac gctcggtgag
     3901 cgcctcgatt tccgccggag cgaggatccc ggtgagccgc tcgcccagct cgccctccag
     3961 cgcggcgacc aggccccgca ggtcgtcgag gagccgcctg gccagccgct ggccggcgaa
     4021 gtcccacagg accgtccgca gcttcggctc gacatggaag cagacgccgt ggtcgatcgc
     4081 ccagacccgg ccgccgcccg agccctggga gcggtcgtcc aggcagtggc cagccttgcg
     4141 gtcggcgttg ttgatcacca cgtcgaaggc ggcgatcagc ggcatggtcg cgtcaccgcg
     4201 ctcgaccagc tcgaaggcgg tcacctccag gtcggcgtcc acgaaccgct gcagcgaccc
     4261 gaccccgaag gggccgtcgt cgcgcgccac cgtcagcggc accagccccc agccgagctg
     4321 ctcgctgacc aggtaggcgg ccacctcgcg gcggtagagg gtgccctcgg ggaagtccca
     4381 cagcggccgc tcgccccgtg ccggcttgta gaccgcgagg ctttccaggc cgtcgtggca
     4441 gatcttggcg agcatggtgg cgttggaggc ccaggggatg cggccgagca cctccagctg
     4501 gccctcctcc agcagcgcac ggacacggcc gtcatcctcg ggcaggggga gggggagggg
     4561 gaccggcggg ggcgctgcca cggaaacctc agctcaggcc agggtcgggc ggtggccgtt
     4621 ttgcgccggg cagatgtggc cggtcgggtc gagcgggttg ccgcacaggg ggcagggccg
     4681 ccggccccgc tccaccacct ggctgccgtg ccgggcgagc acccggagct gcgggcgcgt
     4741 cacccaaagc cgcacctccc gcggctcgcg ctccagcgcc gcctcgagct cgccctcgag
     4801 ctcgagatcg agttcgaggg cggcctcctg ctcgtcgccg gtgtcgggca gcagctcggt
     4861 ggcgaccacc aggatccggt cgcgatcggg gtcgtaggtg atcgccagct ggccgacacg
     4921 gaagtccggc tcgaagggct cggccagaga caggtcgctg gcctcggccg cgcggacctc
     4981 ctccgggtcc tccggccggt cagccgcgag ctccggcagc caggcgatca gccgctcggc
     5041 cagcacccgc acctgctcct tctccaccag cagggtcacc tgctccggcc cggaggccgc
     5101 ctggatcagg aaggtccgct gccccggctc gccgaccgcg tcggcggtca gccgggtgac
     5161 cggatccaga tcacggctgg ccatcgacag gatcctccaa caaccgtaac tgctccggct
     5221 ggggcggctg gggcggcacc tccagagcgg tgtcgtcaag tccgacgtcg ttcagccgca
     5281 gcaggtacgg cagcggcgcg aacccgaacg cggtcaagct cgccggcgcc accaccagcc
     5341 gctggtactg gtccagatgc atccccaggt agtgggcggc gaccgccttg atcacgtcgg
     5401 cgtggctgca caccgccacc gtctggcggg gatgcgccgc ccgcagccgc tccagcgccg
     5461 cgaccgcgcg ggtctgcatc tcgtagagcg actcgccggg cagctccccg tcggggaagc
     5521 gcatcgccga cgggttcccc tgcacgacgc gccacagcgg cagcttggcc aggtcgcgca
     5581 gcgcccggcc ggtccagtcg ccgtagcgga cctcgccgag gtcgggggcg acggtgacct
     5641 ccaagccctt aacgcttgcg accgccgcgg cggtctcctg gcagcgttcc agcgggctgg
     5701 cgtagagcgc gtcgatcttc agcggcgcca gccgctcggc gagcgtcttg gcctgctggc
     5761 ggccctgttc ggacaggtgg aggccgggca gccagcccga gagccgcttg ccggtctcct
     5821 cggtcaccgc gtgtctgagc agcagcagac gatccatcta gtgagtgtag cgagaggggc
     5881 tggcattatt cgtggaggat ccgcccctcc atgccgcccg ccaccgtcac gatctggcca
     5941 ctcacatggc cggaggcggt gtcggacgcg agcagcacca cctgtgtggc gacgtcggtg
     6001 acggtggcga gcttgcgcag cggcatcgtg cgggtgacgc gcgggaccag ggtggggtcc
     6061 tccagcgccg cgcgggtcat tggggtgcgg acccagcccg ggcagacggc gttgacgcgg
     6121 ccgcgcgggg cgatccgcac gatctcgttc ttgagggagc gcaccaggcc gaagatgacc
     6181 gccgacttgg cggcggcgta gtcggcgtgg tcggcctcgc cgaagatccc ggcggtcgag
     6241 ccgatgaaca ccaggttgcc gtgctcctgg cgctgggttt cgagctggcg caggaacgcc
     6301 ccggcggcca ggaaggcggc ggtgaggtcg acccgcaggg tggcctcgat ccgctccggg
     6361 ctgagccgcc acagcggcag cggctccggc ggccacaccc cggcgcagtg gaccaggatg
     6421 tcgagcccgc cgaaggcgtc ggaggcggca ctgaccgcgg cctccgcgcc ctccaccgtg
     6481 ccgaggtcgc caccgaccgc caccgcgctg ggaccggggc cgggcccggg gccggggagc
     6541 tcggcggcga ggcgtttggc ggcctgctcg ttgcggtggt ggtggaccgc gaccagcgcc
     6601 ccctcggcgg cgagggcgcg gcagatcccc tggccgatcc cgccggaccc gccggtgcag
     6661 aggacgcgct tgccctccag gcccaggtcc atcgatcagc cgcccgtcgg ggcggcggcc
     6721 ggggctggtt ccggcggtgt gccgaggccg ttgcgcgagg cccaggcggc gatctcggcg
     6781 cgggaggtca tgccgagctt ggcgaggatg ttggagacgt gcaccgaggc ggtcttggtg
     6841 gagatgaaca gccgccgggc catctcgccg ttggagaggc cctcgaccag cagcgcggcg
     6901 acctcgcgtt cgcgcgcggt gagcacgtcg gggcccgcga ccggctggcc gtcgccgagg
     6961 cggcgcagta gcgccctggt ctcgtcgcgg cgccagccgg gccagcgctc cagcagccgg
     7021 ctggcctggc gggcgtgctc gcgcgcctcg gcgtggcggc cgagcgcgag caggcagcgg
     7081 gccgcgccga ggtggcagtc ggagcgcagg tgcgggatgc ggtagccctc cagcccggcg
     7141 gcgcgcaggt agccctcggt cgcggcggcc aggtcgccct cagcctccag cagggcggcg
     7201 ttcaggtgga cccggagtgg cagcccctcg acccaggcgc ggtcttccgg gctgctcgcg
     7261 gtcacgccgg tcagctcgtc ggctctggcg agcatcgccc gcacctccgc cgctggcaga
     7321 ccggcgcgac cgcgtcgatc agcccgatgt tgggttcggg gccgaggccc caccaccagg
     7381 cggcgacctg cgcgtccgcc tgggcgcacg cggcgcgggc agcgtcgaga tcgccgcggc
     7441 gcgcggccag cgaggcgcag atcgcccaga accacacccg gtcgcactcc tgggaggcgg
     7501 tgagcatcat ctcgcggctt cgtcccagca gctcctcggc cgctggcagc tcgccgccct
     7561 cgatccgcag gaccgcctcg taggggtcga ggaggaactc gaagacgctg ctcgggttgc
     7621 gcgtgcgggt caggcgccgg cccttggcga tcgcggtgag ggcggcgtgc cggtcgcctt
     7681 ccatgacgtg gatctccgac cgccggatcg ccagcccgac gttgtcggcg gcgtggccgg
     7741 ctcgttccga ggcggcgcgg agctgccggt agacctgcca gctccgctcc agcggccagg
     7801 actgcacctg gttgaccatc aggttccaca gcgcccggtg cagggtcagg tcgaagtcgg
     7861 ccgcctccgc ctccgccacc gcacgctcca gcagggcgag gccctcctcc cggcgtcgcc
     7921 ggctgggcgg gccgttgggt tccaccatcg cggtgccctt gttgacctgg gcggcgagca
     7981 ggacggagcg cgggccgacc cgctcggcca gggcgatcgt atgatcggcc cactcgatcg
     8041 cggcggtggc gtcgtcggcc agcatgtacg cctccgagac ccgcgcctgc gcccgcgcca
     8101 gcagcggtga ggggccgagc cgccgcgccg cctccagcgc ctgccagacg cactcccact
     8161 ggcggtcggg gtggtgcgcc tcccagtgca cccgggcgag gtggatcagg acggcggctt
     8221 ggtcctcaag gtcgccgcgc tgccgggcga cctccaggcg gcgctcgccg tgctcgacgg
     8281 agacgtccag cagcccgatc agccaggcgg ccttggacgc ccaggcgagc agcgccgggt
     8341 cgtcgggagc ctcggcgagg ccgtgctcgg ccaggcgcag cgcgtcgacg ctggcaccgc
     8401 ggcagacata ggcctccgcg ccggcgcggg ccgcggcgac cagctcctcg ccacggccgg
     8461 cgccctgggc gtgccagacg atcgcgctcc agtcgtcgga gccggcctcc tgcagcgcgg
     8521 ccagcgcctt ctcgtgcagc cggcggcgct cccggccgag gagctgcccg gcgatcgcct
     8581 cgcgggtgag ggcgtggcgg aaatggaaca cgtcctgctc ccgctcggcc agcaggtcct
     8641 ccctgaccag gcggcgcagc gcctcgatca gctcttgctc gctgcggccg gcgacgctgg
     8701 cgagcagatc gaaggggatc cgctcgccga ggatggcggc ggtctcggcg acctggcgct
     8761 ccggcggctc caggtcgtcc aggcggcgca gcaccgcctc ggtcaggttc cacggcagcg
     8821 gcagcgacgc cagccgctcc ggtgccgcgg tggcgtcgct gcccttgagg tccttggcag
     8881 cggtggcgag cagctcctcc aggaagaagg ggttgccgcc ggtgcggcgg tgcagcgtct
     8941 cggccacccg gaaggggacc gggccgccgt ggaccgcctc caccagctcg tggacctgcg
     9001 cctggtcgag gcgctccagc aggatgctgg tgaccgggcg ctggcggtcc agctcggcga
     9061 gcagctccag catcgggtgg cggcggtcga ccccttctgg ccggaaggtg ccgatcagca
     9121 ggacgtcgag gtcagcggtg agggcgaggc ggccgaacag ggcgacgctc tgggcgtcgg
     9181 cccagtgaag gtcctcgaag accagcaggg tgggtgccag ctcccggatc aggtcgacgg
     9241 cggtgccgag cagctcctcc tggccatact cgcggtcgcg ctcccggtcg cacgccagcc
     9301 gcggggcgat cgggtagagc atcatccgca gggcgtcgtc gcggtcggcg agccgcgcgg
     9361 gcaccgcctc ccagtcgcgc acctcgctgg cgaccgcctc gcgcagcagc tggaagggcc
     9421 ggccgggcat cccctggccg gcctggccgc cgagcaccag cgcgtccggg tccgggtcga
     9481 ggccggcgat cagctccttg agcaggcgcg tcttgccgat gcccgcctcg ccgctgatca
     9541 gggccacgcg ggcgtcaggg accgagcggg cggcgagcag gcggcgcagg cgcgccagct
     9601 cgctggcccg cccgaccatg accgggctga gcccgttccg acctgccagc atacctggca
     9661 ggttaccagg catttaggac actcgcgccg ccatcgcgcg gcttgctcag gcgctcttcg
     9721 cgcgcggcga aaggcggccg gcgccagccc cagcgctcgc gcccgggctt ggcgccgggc
     9781 ttggtgcggc acggctccgc aggctcagga accgtctggc gcccttgcgg gccgccttct
     9841 ggttccgggc caggcgccgg gcgcgcgcgt cgtcgaacag gccgcggcgg cgttcttccg
     9901 cgagcacgcg ggtcaattcg gggtggtact gcatcatgct gctccttcga tgtgcttgat
     9961 ggcttcctgt ggtgccacga tgcgcgtctg aggtagcccg tcgcatcggg c
//
LOCUS       DASWDC010000325        13666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_647564, whole genome shotgun sequence.
ACCESSION   DASWDC010000325 DASWDC010000000
VERSION     DASWDC010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13666
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647564"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1042)
                     /locus_tag="VF995_10440"
     CDS             complement(260..1042)
                     /locus_tag="VF995_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004947293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9344019.1"
                     /translation="MSGGPVLELSGISKRFGAVDALTGVDLRFYPREVVALVGDNGAG
                     KSTLVKVMAGVEPADSGEIRWQGRRVAIRRPHDAQALGIVAVYQDLALCDNLDVVANL
                     FLGAEEGTAGVLNAAAMERQARELLGRLSIQLPDVHAPIASLSGGQRQAVAIARSLLG
                     HPKLVILDEPTAALGIAQTAHVLDLIERLRDQGLTVVLVTHNLADVRAVADRAVVLRL
                     GRNAGDYRITDTSQQEIVAAILGAASTIRPRRPGRLIRSTPG"
     gene            1187..2320
                     /locus_tag="VF995_10445"
     CDS             1187..2320
                     /locus_tag="VF995_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX9344020.1"
                     /translation="MRRANQLRVLGAIRMAGEPTQAEIARLTGLSAATVSNIVRELRE
                     VGTVVVSPTSSSGRRAQSVALLRPVGVSVGVDLGASHLRVAISDLGERVVAEETIAYD
                     VAASAERGIRRAVWLVETLLTQTRVDRSQVIGVGVSVPGPVDRTTGAIDSATILPGWA
                     GARPAEELRERLGMPVHLDNDANLGALGELVGGAGRGCSHLVYLQLSNGVGAGIVADG
                     RLYRGAGGIAGEIGHITVDARGPVCRCGNRGCLETLVGGPYLLQLLSRPTASEEPPLT
                     LGSVIASALAGDPGCRRILSDAGHAVGSGVAILANLFNPRLVIVGGELARAGDLLVQS
                     IRQTAEQGAIPSAVRGLQVVRGKLGERAQVVGALALAATATQA"
     gene            2590..3663
                     /locus_tag="VF995_10450"
     CDS             2590..3663
                     /locus_tag="VF995_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX9344021.1"
                     /translation="MITLVGAACSTTKNSGGGSSSGGAKKIALLLPESKTTRYEAQDK
                     PLFEAKVQALCSDCQIIYNNANQDAAAQQNQADAALTNGAKVLVLDPVDSASAASIVS
                     KAKAKGVPVISYDRLILNADEDYYISFDNVQVGKLQAQSLVNKLKADGKSGSIVMING
                     SPTDNNAKLFKQGAHSVLDTSGFKIAKEYDTPDWSPDKAQTEMEQAITGLGKNAFIGV
                     YAANDGTGGGAIAAMKGQGIAPSTRPTTGQDAELAAIQRILTGEQYMTVYKAIKPEAE
                     DAAQLAVNLVNGNTSAASGMAAQKTNNGQKDVPSIILTPIPVTKATAKETIGKMISDG
                     FLKASDICAGAAASACQAAGISG"
     gene            3772..4557
                     /locus_tag="VF995_10455"
     CDS             3772..4557
                     /locus_tag="VF995_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9344022.1"
                     /translation="MASTPLLELRGVNKRFGAVQALSEVDFAAPAGEVTALVGDNGAG
                     KSTLIKIISGIYAPDQGEYLFEGKPVQVNGPQAAVDLGIATVYQDLALADNLDVVANL
                     FLGRELIAPVRRLDETVMEREANQVLDSLAVRIPSVRTQVAGLSGGQRQSVAIARSML
                     GEPKVVLLDEPTAALGVAQTAQVLSLIRRLRERGLAVVIISHNLADVFEVADRIEVLR
                     LGRRVAVFAARQTSSDEVVSAITGAKAGEERLATDINMEEAER"
     gene            4608..5828
                     /locus_tag="VF995_10460"
     CDS             4608..5828
                     /locus_tag="VF995_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9344023.1"
                     /translation="MTARDVDPRLISDQPGLAGMVAGFRRRVSQGELGPLPVIIGIAI
                     IWVIFQVANHNFLAPGNLTNLVLQMAAGGTISVGVVLVLLLGEIDLSVGALSGLAAAV
                     MAVLNVKHGVPGWLAILAGLATGTLIGLVQGLWVTRFRIPAFVVTLAGLLAWTGGLIF
                     VLGETGTVNLADKNITGLANTFFIPLDAWLLAVLVTAAYTVERLWDRRQRVAARLPVP
                     PLSVLVTRVALLAVALCAVAAVFSADRGLPLAAVIFVGLVVAFDLLIKRTRFGRHILA
                     VGGNTEAAHRAGIPVENVRLAVFVLASTMAAAGGIIAASRLFAVNQSSGGSDTLLNAI
                     AAAVIGGTSLFGGRGSAYSALLGSLVIYSIQNGMDLLGVSSDKKFMVTGLVLLLAATV
                     DALSRRGREAHGRA"
     gene            5902..6900
                     /locus_tag="VF995_10465"
     CDS             5902..6900
                     /locus_tag="VF995_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEX9344024.1"
                     /translation="MVDLAVRQQMAVAATAWLDSLSPAQRRKAYYGFPATEERTRWFY
                     TPNEQGGLPLAEMDPVQQRLAHRLLASGLSRPGYTTAATIMGLENPLDASEGWRRHYA
                     GRAVPNRGRDPQLYYLTVFGDPDAGAAANGDAAWGWRAGGHHLSVSYTLVGNQVAASP
                     LFFGANPASIALVGPGLLRPLAGEEDLARELLAALSPQERAAALLSEVAPDDILQFNR
                     SMVRDLGDPVGLAAGELTTAQQKLLAALVRQYLDRLPDEVASAYAERLSLSKQGAAFA
                     AIHFAWAGGSTPGQGHYYRLQGPRLLIEYDCTQNHANHIHAVLRDPDGDFGADLLA"
     gene            6990..9071
                     /gene="paaZ"
                     /locus_tag="VF995_10470"
     CDS             6990..9071
                     /gene="paaZ"
                     /locus_tag="VF995_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HEX9344025.1"
                     /translation="MLLSSYAQGRWYTAPDTDTGTPLLHAVTGAEVARISSAGLDVAG
                     MLDHARRVGGPALRAMTFHQRAAMLKALAQYLSDPGRKQEFYALSAATGATPADSALD
                     IDGGIGTLFVYSSKGRRELPNDTVYLDGPPEPLSRRGTFAGQHLYVPLRGVAVLIDAF
                     NFPVWGMLEKLAPALLAGVPVIVKPASQTAYLTELAFRRMVESGLLPDGAAQLICGSV
                     GDLFEHLTGQDAVSFTGSASTALRLRGHPVVLRNSVRFNAEADSLNCSVLGLDAKPGS
                     DEFDLYVREVVREMTVKAGQKCTAIRRALVPREYADAVVEALREQLGKVRVGDPAEPG
                     VTMGALASLGQRDEVRHAVERLSGAAELLQGTLDPGSFRVEGADPERGAFLPPILLFG
                     GDPDRDELHTVEAFGPVSTVLPYQSAAHAATLAARGEGSLVGSIFSDDDAFVRQLALE
                     AAAHHGRLLLVNRECAAEQTGHGSPLPHLVHGGPGRAGGGEELGGVRGVLHYMRRTAL
                     QGSPTTLTAVTGRWMPGAARVEDPAGVHPFRKSLEDLRIGEALHTGTRTVTLDDIERF
                     AASTGDRFYAHMDEQAAARNPFFDGRVAHGYLIVALAAGLFVDPDEGPVLANYGLENL
                     RFTKPVYPGDTLRVAFTCKEKNPREAEPYGEVRWDTEVSNQDGEVVARYDVLTLVAKR
                     PKPGADDGPGS"
     gene            complement(9295..9543)
                     /locus_tag="VF995_10475"
     CDS             complement(9295..9543)
                     /locus_tag="VF995_10475"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF005894.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX9344026.1"
                     /translation="MTALEELRAAMDEVDRELQHLAARRRALSQQIQAVRLRDGGDRH
                     DLDREQQVVASYLEALGPDAAALAEALLRLCRGPLASS"
     gene            complement(9540..10436)
                     /locus_tag="VF995_10480"
     CDS             complement(9540..10436)
                     /locus_tag="VF995_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344027.1"
                     /translation="MEMVTASSTSAAPTEPPPLLAGGVTIGAGTFTLIARAAASREGA
                     GAAALASGQAAELAQLARGARAALLDVGHAWVSAGTVEDTLDLGLPLVCEVGMAAEVP
                     AAARSAALLRVHVQVQAREGVAPGSSMGVALLRALGRAELPVLLDLDLVPDSDGGAPA
                     EGSLGVIRHLAGMVGEAVGGQVALCLPGAGRAFDLAAIPSLRAASGWPLLVDATGADP
                     RRAAALARAAAAVGADGVVVAAGEPIAGLAEAELARLSGELDAVAGALGRRMGGRRIR
                     YVGRGGDERRDQYRGRDREWRA"
     gene            10841..12202
                     /locus_tag="VF995_10485"
     CDS             10841..12202
                     /locus_tag="VF995_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9344028.1"
                     /translation="MPSPSGPGSLTGFPWPVESARLGNGLWVIVSEDHTAPVVAVNLW
                     YDVGSRHEPTGQTGFAHLFEHLMFEGSLHVPKAEHMRLIQGSGGSLNATTNPDRTNYF
                     ETVPAEHLELALWLEADRMGALVPALTQETLDNQREVVKNERRQRYDNVPYGDAWLRL
                     LALLYPPGHPYHHATIGSMADLDAADLDTFKAFHTTWYAPNNAVLTVVGDTDAATVFA
                     LAERYFAGLAPNPDLPPPPSGSDGDASATGEVAAPIEREARETVVAEVEAPRVYLATR
                     TAPFGSVAYDAVTLLATVLGSGRGSRLYQRLADGARLAQPDSVAAYSVDLAHAPAPLL
                     LTATTRPGVTAEALEAGLAELVDELAAGNPAVAVTAAELDRAKALLTTGWWQQVATVG
                     GRADILGQYATQLGDPARAGEQLPAWLAVTTDQVAEAAAAVLQPHDRVTLTYLPAAKE
                     AAA"
     gene            12301..13590
                     /locus_tag="VF995_10490"
     CDS             12301..13590
                     /locus_tag="VF995_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9344029.1"
                     /translation="MPRGQGQARGQVVAAHLPGQPLAVATLLLGASASHEPTGREGLA
                     SVLARALEEGTAARDAAAYALALEGLGAELFTVVDWDSFRVGVEAPVERLPAAVELLA
                     EAARTPRLDPADVTRVRDDEVTSLRMWWAEPGPRADAALRADLFGADQRHGRPVHGDP
                     ASVAAVSADEVAAYHQAWLTRPGALLVAGDLDRLDLDALGGAAFAGTSAQPAGADGPA
                     ATTARPARRILLVDRPGSVQSTLRLGHTAPPRAHPDYVAMSLAGTVLGGAFTSRLNHL
                     IREVRGYTYGIRADFAHSRRFGAFTVRTAVQTEVTAPALADAVAEISRTHADGVTDAE
                     LEVARSFRAGQLSVDMQTPGAIARALTTLVIHGLPDDYHATLRNHLLNATVEEVSAAA
                     ATHLRPDRLTLVVEGDAAAIRDQLAVTGLGDLADATV"
BASE COUNT         1904 a         5151 c         4732 g         1879 t
ORIGIN      
        1 gcgaaggtgg cgggcccggc gaagcggggg acggcgcggg cgtcggctgg gccgagcggc
       61 gcctgggtgg gcgggtcgtt cagctgccgg ccgaccatgc aagcgggcga agcaatgccg
      121 tccatgggag gccggcagag cccgtgccgg gcgtcctcgt cacggaaatg gcggggatcg
      181 tgcccttggc ggtgctcggt caccgtctgc ccctttcgct ccaggtcgga ggccgcggcg
      241 tggctgctgc tcggttcgct cagcccggtg tcgagcgtat caagcggcca ggccgccggg
      301 gacggatggt gctggcggcg ccgaggatgg cggcgacgat ctcctgctgg ctggtgtcgg
      361 tgatgcggta gtcgccggcg ttgcggccga gccgcaggac gacggcgcgg tcggcgacgg
      421 cgcggacgtc ggcgaggttg tgggtgacca ggacgaccgt cagcccctgg tcgcggaggc
      481 gctcgatcag gtcgagcacg tgggcggtct gggcgatgcc gagcgcggcg gtcggctcgt
      541 cgaggatcac cagtttgggg tggccgagca gggagcgggc gatggcgacc gcctggcgct
      601 gcccgccgga gaggctggcg atcggcgcgt ggacgtccgg gagctggatc gagagcctgc
      661 cgagcagctc acgggcctgg cgctccatcg cggcggcgtt gaggaccccg gcggtgccct
      721 cctcggcgcc gaggaacagg ttggcgacca cgtcgaggtt gtcgcacagc gccaggtcct
      781 ggtagacggc cacgatcccg agggcctggg cgtcgtgggg gcgccggatg gccacgcggc
      841 ggccctgcca gcggatctcg cccgagtctg ccggctcgac gcccgccatc accttgacca
      901 gggtcgactt gccggcgccg ttgtcgccga cgagggcgac cacctcgcgg gggtagaacc
      961 tcaggtcgac gcccgtgagc gcgtcgacgg cgccgaaccg tttcgagatc cctgacagct
     1021 ccaggacagg ccccccgctc atgccatcaa tccttccacc tccactggat ctccgcaggg
     1081 gatgcctgct tgtgttgact tcaacgcttg taagctaatc atgcatgatg gagcgggagg
     1141 tgacatgacg gctgataccg cgtcaacacc gggatcgcaa tcggccttgc gccgggcgaa
     1201 ccagctccgc gtgctcggcg cgatccggat ggcgggggag ccgacccagg cggagatcgc
     1261 ccggctcacc ggcctgtcgg ccgccactgt ctccaacatc gtgcgtgagc tgcgcgaagt
     1321 cggcacggtg gtagtcagcc cgacctcgtc gagcggccgc cgcgcgcaga gcgtcgcgct
     1381 gctccggccg gtcggggtca gcgtcggcgt cgacctcggc gcgtcgcacc tgcgggtcgc
     1441 catcagcgac ctcggcgagc gggtcgtcgc cgaggagacc atcgcctacg acgtggcggc
     1501 cagtgccgag cgaggcatcc gccgcgccgt ctggctggtc gagacgctgc tgacccagac
     1561 gcgcgtggac cgctcgcagg tgatcggcgt cggcgtcagc gtccccgggc cggtggaccg
     1621 gaccaccggc gcgatcgact cggcgacgat cctgcccgga tgggcggggg cgcggccggc
     1681 cgaggagctg cgcgagcgcc tcggcatgcc ggtccacctg gacaatgacg ccaacctcgg
     1741 ggcgctcggc gagctggtcg gcggtgctgg ccggggctgt tcgcacctgg tctacctgca
     1801 gctctccaac ggtgtcggcg cgggcatcgt ggccgacggg cgcctgtacc ggggcgcggg
     1861 cggcatcgcg ggcgagatcg ggcacatcac cgtcgacgcg cgcggtcctg tctgccgctg
     1921 cggcaaccgg ggctgcctgg aaacgctggt cggcggcccg tacctgctgc agttgctgtc
     1981 acggccaacc gcgtcggagg agccgccgct gaccctgggg tcggtgatcg caagcgcgct
     2041 cgcgggtgat cccggctgtc ggcggatcct gtccgacgcg gggcacgcgg tcggcagcgg
     2101 cgtggccatc ctcgccaacc tcttcaaccc ccggctggtg atcgtgggcg gcgagctggc
     2161 ccgggcgggg gacctgctgg tgcagtcgat ccgccagacg gccgagcagg gcgcgatccc
     2221 gagcgccgtc cgtggcctcc aggtcgtccg cggcaaactg ggcgaacggg cgcaggtcgt
     2281 cggcgcgctc gccctcgctg ccaccgccac gcaggcctag gcggtcggcg cgatcgagcg
     2341 ttttggcttg tcaaaggtcc gcttctgttc acctcttgaa tgaacaatcc gtttctgcgt
     2401 aggctttcgt tcaagtcctt gacttgatat gccgctcatc gcagggaggt gacaccgcag
     2461 gcaccgagag cgtcgtcgcg ccgtggcgcg gagcaggctc ccggccggga gcccttaccc
     2521 agaagtgagg tggcaaggtt gccaggcaag tccatcggcc gagcgttccg gctcgccgcc
     2581 gtcgtggcca tgatcacact ggtcggagcc gcttgctcca ccaccaagaa ttccggaggc
     2641 ggcagctcgt cgggcggcgc caagaagatc gccctgctgc tgcccgagag caagacgacc
     2701 aggtacgagg cgcaggacaa gccgctgttc gaagccaagg tccaggcgct ctgctcggac
     2761 tgccagatca tctacaacaa cgccaaccag gacgccgccg cacagcagaa ccaggccgat
     2821 gccgcgctca ccaacggcgc caaggtgctg gtcctggacc cggttgactc ggcctcggct
     2881 gcctcgatcg tctccaaggc caaggccaag ggcgtcccgg tgatcagcta cgaccggctg
     2941 atcctcaacg ccgacgagga ctactacatc tcgttcgaca acgttcaggt cggcaagctg
     3001 caggcccaat cgctggtcaa caagctcaag gctgacggca agtccggctc gatcgtgatg
     3061 atcaacgggt caccgaccga caacaacgcc aagctcttca agcagggcgc ccacagcgtc
     3121 ctcgacacca gcggcttcaa gatcgccaag gagtacgaca ccccggactg gagcccggac
     3181 aaggcccaga ccgagatgga gcaggcgatc accgggctcg gcaagaacgc cttcattggg
     3241 gtctacgccg ccaacgacgg caccggcggc ggcgcgatcg cggccatgaa aggccagggc
     3301 atcgccccct cgacccggcc gaccaccggc caggacgccg agctggccgc gatccagcgc
     3361 atcctcaccg gcgagcagta catgaccgtc tacaaggcga tcaagccgga ggccgaggac
     3421 gccgcgcagc tcgcggtcaa cctggtcaac ggcaacacct ccgctgccag cggcatggcc
     3481 gcccagaaga ccaacaacgg ccagaaggac gtgccctcca tcatcctcac gccgatcccc
     3541 gtgaccaagg cgaccgccaa ggagacgatc ggcaagatga tctcggacgg cttcctgaag
     3601 gcgtccgaca tctgcgccgg cgcggccgcc tcggcctgcc aggccgctgg catcagcggc
     3661 tgagggacgt acgctggtcc caggcgccag ccgggacgtc gcacggaggt acgggccggc
     3721 cgcttgtcag ggtccggccc gtacctcctg aagccggaag gaggccagct ggtggcgagc
     3781 acgcccctgc ttgagctgcg cggggtgaac aagcggttcg gtgcggtgca ggcgctcagc
     3841 gaggtggact tcgcggcgcc tgccggcgag gtcaccgccc tcgtcggtga caacggcgcg
     3901 ggcaagtcca ccctgatcaa gatcatttcg ggcatctacg cgcctgatca gggcgagtac
     3961 ctgttcgagg gcaagccggt gcaggtcaac ggcccgcaag cggccgtgga cctgggcatc
     4021 gccaccgtct accaggacct ggccctggcc gacaatctgg atgtggtcgc caacctcttc
     4081 ctcggcaggg agttgatcgc gcccgtccgc cggctcgacg agaccgtcat ggagcgcgag
     4141 gcgaaccagg tcctcgacag cctggccgtg cgtatcccga gcgtccgcac ccaggtcgcc
     4201 ggcctctcgg gcgggcagcg ccagtcggtc gcaatcgccc ggtcgatgct gggcgaaccc
     4261 aaggtcgtcc tgctcgacga gcctaccgcc gccctcgggg tggcacagac cgcgcaggtg
     4321 ctgagcctta tccgccgcct tcgtgagcgc gggctggcgg tggtgatcat cagtcataat
     4381 ctcgccgacg tcttcgaggt cgccgaccgg atcgaggtat tgcggctggg tcgccgggtg
     4441 gcggtgttcg cggccaggca gaccagctcc gacgaggtcg tctcggcgat caccggcgcc
     4501 aaggccggcg aggagagact cgccaccgac atcaacatgg aggaggcgga gcggtgaccg
     4561 ccaggatggg cgctgcaagc tggcgaacaa tgaccggggg tgcatgaatg accgcccgtg
     4621 acgttgaccc gcggctcatt tccgaccagc ctggcctcgc cgggatggtg gcaggtttcc
     4681 gccgccgcgt ctcccaggga gagctcggtc cgctgccggt catcatcggc atcgccatca
     4741 tctgggtgat cttccaggtc gccaaccaca acttcctcgc ccccggcaac ctcaccaacc
     4801 tcgtcctgca gatggctgct gggggcacga tctcggtcgg agtcgtgctg gtgctgctgc
     4861 tcggcgagat cgacctgtcg gtcggggcgc tcagcgggct cgccgccgcc gtcatggcgg
     4921 tcctcaacgt caagcacggc gtccccggct ggctggcgat cctggccggg ctcgccaccg
     4981 gcaccctgat cgggctggtc caggggctgt gggtgacccg gttccggatc ccggccttcg
     5041 tcgtcaccct ggccggcctg ctcgcctgga caggtgggct gatcttcgtc ctcggcgaga
     5101 ccggcacggt caacctcgcc gacaagaaca tcaccgggtt ggccaacacc ttcttcatcc
     5161 cgctggacgc ctggctcctg gccgtgctgg tgaccgccgc ctacaccgtc gagcggctgt
     5221 gggatcggcg ccagcgggtc gcggcgaggc tgccggtgcc gccgctcagc gtcctggtga
     5281 cgcgggtggc gctgctcgcc gtcgcgctct gcgccgtcgc cgcggtgttc tctgccgacc
     5341 gtgggcttcc gctggccgcg gtgatcttcg tcggcctggt ggtggccttc gatctgctca
     5401 tcaagcggac ccggttcggc cggcacatcc tggccgtcgg cggaaacacc gaggccgccc
     5461 accgcgccgg tatcccggtg gagaacgtgc ggctcgccgt gttcgtgctc gcctccacca
     5521 tggccgcggc cggtgggatc atcgcggcgt cgcggctgtt cgcggtcaac cagtcgtcgg
     5581 gcggcagcga cacgctcctc aacgccatcg cggcggccgt gatcggcggg accagcctgt
     5641 tcggcggccg tggctccgcc tactcggcgc tgctcggctc gctcgtgatc tattcgatcc
     5701 agaatggcat ggacctgctc ggcgtcagct ccgacaagaa gttcatggtt accgggctgg
     5761 tactgctgct ggccgcgacc gtcgacgcgc tgtcccgccg cggccgcgag gcccacggcc
     5821 gcgcctagct ggatggatcg ctccgttcgt cgcgacggtt catgagcgac gcattcggtt
     5881 gcaccaggta ccatcgtggc gatggtggac ttggcggtgc gccagcaaat ggcggtagcg
     5941 gccacggcgt ggctggattc gctcagcccg gcgcagcggc gcaaggcgta ctacggcttc
     6001 cccgccaccg aggagcgtac ccgctggttc tacaccccca acgagcaggg cggcctcccg
     6061 ctggccgaga tggacccggt gcagcagcgg ctcgcgcacc ggctgctcgc cagcggcctc
     6121 agccggccgg gctacaccac cgcggcgacc atcatgggcc tggagaaccc cctcgacgcc
     6181 tcggaggggt ggcgccgcca ctacgcgggc cgggccgtcc ccaaccgtgg ccgcgacccg
     6241 cagctctact acctcacggt cttcggcgac cctgacgcgg gggccgccgc caacggcgac
     6301 gccgcctggg gctggcgtgc cggcggccat cacctgtcgg tcagctacac cctcgtcggg
     6361 aaccaggtcg ccgccagccc gctatttttc ggcgcgaacc ccgcgtccat cgcgctggtc
     6421 ggcccggggc tgctgcgccc actggcgggg gaggaggacc tcgcccggga actgctggcc
     6481 gccctctccc cgcaagagcg ggccgccgcg ctgctgtcgg aggtggcacc ggacgacatc
     6541 ctgcagttca accgttcgat ggtccgcgac ctcggcgacc ccgtgggcct ggcggcgggg
     6601 gagctgacca ccgcccagca gaagctgctg gcggccctgg tccgccagta cctcgaccgc
     6661 cttcccgacg aggtcgcctc cgcgtacgcc gaacggctgt cgctcagcaa gcagggtgcg
     6721 gccttcgccg cgatccattt cgcctgggcg ggcgggagca cccctgggca gggccattac
     6781 taccgcctcc agggcccgcg cctgttgatc gagtacgact gcacccagaa ccacgccaac
     6841 cacatccacg ccgtcctgcg tgacccggac ggcgacttcg gcgcggacct gctcgcctga
     6901 cgcccttccc agcctgccca ccggccgcac cagcacctat cctggcattc gcgacgctcg
     6961 ggtgatcgga agcgaaaggc ggcacggcaa tgttgctgag cagctacgcg cagggacgct
     7021 ggtacaccgc accggacacc gacacaggga cgccgctgct gcacgcggtg accggtgcgg
     7081 aggtggcgcg gatctcctcg gcgggcctgg acgtcgccgg gatgctcgac catgcccggc
     7141 gcgtcggcgg gccggcgctg cgggcgatga cctttcacca gcgcgccgcg atgctgaagg
     7201 cgctggcgca gtatctctcc gacccgggcc gcaagcagga gttctacgcc ctctcggccg
     7261 cgaccggcgc gacccccgcc gactccgccc tcgacatcga cggcggcatc ggcaccctgt
     7321 tcgtctactc cagcaagggc cgccgcgagc tgcccaacga caccgtctac ctggacggcc
     7381 cgccggagcc gctgagccgt aggggcacct tcgccggcca gcatctctac gtccccctcc
     7441 gcggggtcgc cgtgctgatc gacgcgttca acttcccggt ctgggggatg ctcgagaagc
     7501 tcgccccggc gctgctcgcc ggcgtcccgg tcatcgtcaa gcccgccagc cagaccgcct
     7561 acctgaccga gctggcgttt cgccgcatgg tcgagtcggg cctgctgccc gacggggcgg
     7621 cgcagctcat ctgcggcagc gtcggggacc tgttcgagca cctcaccggg caggacgcgg
     7681 tcagcttcac cggctcggcc tcgaccgcgc tgcggctccg cggccatccg gtggtgctgc
     7741 gcaactcggt ccgcttcaac gccgaggccg actcgctgaa ctgctcggtg ctcggcctgg
     7801 acgccaagcc cggcagcgac gagttcgacc tgtacgtccg cgaggtcgtc cgcgagatga
     7861 ccgtcaaagc gggccagaaa tgcaccgcga tccgccgtgc cctggtcccg cgcgagtacg
     7921 ccgacgcggt ggtcgaggcg ctgcgcgaac agctcggcaa ggtccgcgtc ggcgaccccg
     7981 ccgagccggg cgtcaccatg ggcgcgctcg ccagcctcgg ccagcgcgac gaggtccgcc
     8041 acgccgtcga gcgcctcagc ggcgccgccg agctgctgca ggggacgctc gaccccgggt
     8101 cgttccgggt ggagggcgct gaccccgaac gcggcgcgtt cctgccgccg atcctgctgt
     8161 tcggtggcga ccccgaccgc gacgagctcc acacagtgga ggcgttcggg ccggtcagca
     8221 ccgtcctgcc gtaccagtcg gcggcgcacg ccgccacgct cgccgcccgt ggcgagggga
     8281 gcctggtcgg ctcgatcttc agcgatgacg acgccttcgt ccgccagctg gcgctggagg
     8341 ccgccgccca ccacggccgc ctgctgctgg tcaaccggga gtgcgcggcc gaacagaccg
     8401 gccacggctc gcccctgccg cacctggtcc acggcggccc gggccgcgcc ggcggcggcg
     8461 aggagctcgg cggggtccgc ggcgtgctcc actacatgcg gcgcaccgcc ctgcagggct
     8521 cgcccaccac cctgaccgcc gtcaccggcc gctggatgcc gggcgccgcc cgcgtcgagg
     8581 accccgccgg ggtgcacccg ttccgcaagt ccctggaaga cctccgcatc ggcgaggcgc
     8641 tgcacaccgg cacccggacc gtcaccctgg acgacatcga gcggttcgcc gcctccaccg
     8701 gcgaccggtt ctacgcgcac atggacgagc aggccgcggc ccgcaacccg ttcttcgacg
     8761 gccgcgtcgc ccacggctac ctgatcgtcg ccctcgccgc cggcctgttc gtcgacccgg
     8821 acgaaggccc ggtgctggcc aactacggcc tggagaacct ccgcttcacc aaaccggtct
     8881 acccaggcga caccctgcgg gtcgccttca cctgcaagga gaagaacccc cgcgaggccg
     8941 agccctacgg ggaggtccgc tgggacaccg aggtcagcaa ccaggacggc gaggtggtcg
     9001 cccgctacga cgtgctcacc ctggtcgcca aacggcccaa gcccggcgcc gacgatgggc
     9061 cggggtcctg acaggaggcg ccgccagccc gtatcgtgaa ggcatgaccg tcggactgga
     9121 ccaaatcggc agcctttcct gaagggtcgc tctggacgag cgcctgcggg tctcgcagga
     9181 gcgtgagcgg ctcagccggg agctgaagct gaaccggcca ggggtgttcg cccgctggct
     9241 ggcatggtgg cgtccccacc aggcgccgcc ggacccagaa cccgctcagg aggctcacga
     9301 ggaggcgagc gggccgcggc agagccgcag cagcgcctcg gccagcgcgg ccgcgtccgg
     9361 gcccagcgcc tccaagtagg aggcgaccac ctgctgctcg cggtccaagt cgtgccggtc
     9421 gccgccgtcg cgcagccgca ccgcctggat ctgctgggag agggcgcggc gccgtgccgc
     9481 cagatgctgc agctcgcggt ccacctcatc catggccgcc cgcagctcct ccagcgcggt
     9541 cacgcccgcc actcccggtc ccggccccgg tactggtccc ggcgctcgtc gccgccccgg
     9601 ccgacatagc ggatgcgccg tccacccatg cgccgcccga gcgccccggc gaccgcgtcc
     9661 agctcgccgc tcagccgcgc cagctcggcc tcggccaggc ccgcaatcgg ctcaccggcc
     9721 gcgaccacca ctccgtcagc cccgaccgcc gccgccgccc gggccagcgc cgcagccctg
     9781 cggggatcgg cgccggtggc gtcgaccagc agcggccagc cgctggcggc gcgcagcgac
     9841 gggatcgctg ccagatcgaa cgcccgcccc gcgccaggca ggcacagcgc cacctgcccg
     9901 ccgaccgcct cgccgaccat cccggcgagg tgccgtatca cccccagcga gccctcggcc
     9961 ggcgccccgc cgtcgctgtc cggcaccagg tcgaggtcga gcagcaccgg cagttccgcc
    10021 cggccgagcg cccgcagcag cgccaccccc atcgacgacc ccggcgcgac gccctcccgc
    10081 gcctggacct ggacgtggac ccgcagcagg gcggcgctgc gcgccgccgc gggcacctcc
    10141 gccgccatgc ccacctcgca gaccagcggc aggcccaggt cgagcgtgtc ctccacggtg
    10201 ccggcggaaa cccaggcgtg gccgacgtcg agcagggcgg ccctggcgcc gcgcgcgagc
    10261 tgcgccagct cggcggcctg cccggaagcc agggccgccg cgccagcgcc ctcccggctc
    10321 gccgcggcgc gggcgatcag ggtgaaggtg cccgccccga tggtcacccc acccgccagc
    10381 agtggcggcg gctcggtcgg tgccgctgag gtcgaggagg ccgtcaccat ctccatgtca
    10441 tcctttcgct gcctgcgtcg cttcttgtcg tccttgtcgt catcggcgcg tgggtggaaa
    10501 ccagagagcc gccccggcgg tatccggggc ggctctctcg tcgagctggc gcgcgaaggt
    10561 aggtcagtgg gcgcatgacg aggaccggga cccggtcctc gtaaatcgaa cgtccagccg
    10621 gcgctcgact gccacaacgc ctcccatgcg tgcgtccgct cgtattgcct gttggtgcaa
    10681 tcctacacaa tggcactgga tccgtgtcgc gggcctgccg tccacctcca cctgggctcc
    10741 cactgaggaa tactgtgtgc gggcggcgtc gccgtaccgt cccttttgtg ccatcacgtt
    10801 gcgcgtcgcc gtgccgcaac gtctgccgtg aggagggtct gtgccgtcac cttctggacc
    10861 agggagtctg accggctttc cctggccggt cgagtccgcc cggctgggca acgggctgtg
    10921 ggtgatcgtc agcgaggacc acacggcccc ggtggtggcc gtcaacctct ggtacgacgt
    10981 cggttcgcgg catgagccga ccgggcagac cgggttcgcg cacctgttcg agcacctcat
    11041 gttcgaggga tccctgcacg tgcccaaggc cgagcacatg cggctcatcc agggttcggg
    11101 cggctccctc aacgccacca ccaaccccga ccgcaccaac tacttcgaga ccgtcccggc
    11161 cgagcacctg gaactggcgc tgtggctgga ggccgaccgg atgggcgcgc tggtgccggc
    11221 gctgacccag gagacgctcg acaaccagcg cgaggtggtc aagaacgagc gccgccagcg
    11281 ctacgacaac gtcccctacg gcgacgcgtg gctgcgcctg ttggcgctgc tgtacccgcc
    11341 ggggcacccc taccaccacg ccaccatcgg gtcgatggcc gacctcgacg ccgccgacct
    11401 cgacaccttc aaggcgttcc acaccacctg gtacgcaccc aacaacgcgg tcctgaccgt
    11461 ggtgggggac accgacgccg cgaccgtgtt cgccctcgcc gagcggtatt tcgccggcct
    11521 ggcgccgaac ccggacctcc cacctccgcc atcagggagc gacggtgacg cctcggccac
    11581 gggcgaggtc gccgcgccga ttgagcgcga ggcccgcgag acggtcgtcg ccgaggtcga
    11641 ggcgccccgc gtctacctgg cgacccgcac cgccccattc ggcagcgtcg cctacgacgc
    11701 ggtgaccctg ctcgccaccg tcctcggcag cgggcggggc agccggctct accagcggct
    11761 ggccgacggt gcccggctcg cgcagcccga cagcgtggcc gcctacagcg tcgacctggc
    11821 ccacgcgccg gcgccgctgc tcctcaccgc caccacccgg ccaggcgtca ccgccgaggc
    11881 gctggaggcc gggctcgccg agctggtcga tgagctcgcc gccggcaacc ccgccgtcgc
    11941 cgtcaccgca gccgagctgg accgggccaa agcgctgctg accaccggct ggtggcagca
    12001 ggtcgccacc gtcggtggcc gcgccgacat cctcggccag tacgccaccc agctcggcga
    12061 cccggcccgc gcgggcgagc agctgcccgc ctggctggcg gtcaccaccg atcaggtggc
    12121 cgaggccgcc gccgcggtcc tccaaccgca cgaccgcgtg accctgacct acctgcccgc
    12181 cgcgaaggag gccgcagcgt gagccgccgg gcgggcgacg cgacctcccg gctggtgccg
    12241 gagccgcctg ggccgggccg gcaacgcccc taccggttcc cgcaggtcgt gcgcgaggtc
    12301 atgccccgcg gccagggtca ggctcgcggc caggtggtgg ccgcccacct gcccggccag
    12361 ccgctcgccg tcgccacgct gctgctcggc gccagcgcca gccatgagcc gaccggccgg
    12421 gaaggcctcg cctcggtgct ggccagggcg ctggaggagg gcaccgccgc ccgtgacgcc
    12481 gccgcctacg cgctggcgct ggaaggcctc ggcgccgagc tgttcaccgt ggtcgactgg
    12541 gactcgttcc gggtcggcgt ggaggccccg gtcgagcggc tgcccgccgc ggtggagctg
    12601 ctcgccgagg cggcccggac cccccggctc gaccccgccg acgtcacccg cgtgcgcgac
    12661 gacgaggtca cctcgctgcg catgtggtgg gccgaacccg gtccccgcgc cgacgccgcc
    12721 ctgcgcgccg acctgttcgg tgcggaccag cgccacggcc gcccggtcca cggcgacccc
    12781 gcctcggtcg cggccgtctc cgccgacgag gtcgccgcct accatcaggc ctggctgacc
    12841 cggcccggag ccctgctggt cgctggcgac ctggaccggc tcgaccttga cgcgctcggc
    12901 ggcgcggcgt tcgccgggac cagcgcccaa cccgccggag cggacggccc ggctgcgacg
    12961 acggcccggc cggcccgccg gatcctgctg gtggaccgtc ccgggtcggt ccagtcgacg
    13021 ctgcggctcg gccacaccgc gccgccccgg gcgcatcccg actacgtcgc gatgtcgctc
    13081 gctggcaccg tgctcggtgg cgccttcacc tcccggctca accacctgat ccgcgaggtc
    13141 cgcggctaca cctacggcat ccgcgccgac ttcgcccact cgcgccgctt cggcgccttc
    13201 accgtccgca cggccgtgca gaccgaggtg acagcccccg ccctggccga cgcggtcgcc
    13261 gagatcagcc gcacccacgc cgacggcgtc accgacgcgg agctggaggt cgcccgcagc
    13321 ttccgcgccg ggcagctctc ggtcgacatg caaacccccg gggccatcgc cagagccctc
    13381 accaccctgg tgatccacgg cctccccgac gactaccacg ccaccctgcg caaccacctc
    13441 ctcaacgcca ccgtggagga ggtctcggcg gcagccgcga cccacctgcg tcccgaccgc
    13501 ctgaccctgg tggtcgaggg cgacgccgcc gccatccgcg accagctcgc cgtcaccggc
    13561 ctcggcgacc tggccgacgc caccgtctga ccggggcgct cagcctcgcg ggcggaccag
    13621 gcccgcgtcg taggcgagga tgaccgcctg gacgcggtcg cgcagc
//
LOCUS       DASWDC010000326         6624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_397195, whole genome shotgun sequence.
ACCESSION   DASWDC010000326 DASWDC010000000
VERSION     DASWDC010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397195"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VF995_10495"
     CDS             <1..484
                     /locus_tag="VF995_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9344030.1"
                     /translation="LDDLVAGANAAGITVRTRIEGDPRRLPAGVDLAAFRIVQEALTN
                     VARHAGPASATVTIRYQGRELAVQVDDDGRGAPPDQRRHHAGPAPGGGNGIRGMRERA
                     EALGGQLAAENRPGGGFRVEAMLPLDGTGPEGGIDPASDTPDAAGEQREEAAERGVPW
                     "
     gene            481..1146
                     /locus_tag="VF995_10500"
     CDS             481..1146
                     /locus_tag="VF995_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010352972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9344031.1"
                     /translation="MIRVLLADDQALVRAGFRALLDAQPDIEVVGEAGDGAAAVELAS
                     TATPDVVLMDIRMPVLDGLAATQKIAADPRLGGVRIIILTTFELDEYVFEAIRIGASG
                     FLVKDTEPADLLRGVRAVAAGDALLSPSVTRRLIEQFATRAKRPRDTGRLNALTDRER
                     EVLALVAGGLSNDEIAERLVLSPLTVKTHVSRTIGKLDIRDRAQLVVLAYETGLVRPG
                     WLG"
     gene            1345..2115
                     /locus_tag="VF995_10505"
     CDS             1345..2115
                     /locus_tag="VF995_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9344032.1"
                     /translation="MTTLPATFTDRQTAPATVAAHTVSATKTYGIGDTAVRALDNVTV
                     AFPSGRFTAIMGPSGSGKSTLLHCAAGLDDLTAGQAVIGGIELGSLSDKQLTRLRREQ
                     VGFVFQSFNLLPTLTALENITLPAALAGTKPEQDWLDVVIDTVGLRERLSHLPAELSG
                     GQQQRVAVARALVRQPRIIFADEPTGNLDSRASAEILNFLRRAVRELDQTIVMVTHDP
                     VAASYADQALFLADGRLVGRMEAPTAQRVLDRIKEAGE"
     gene            2128..4698
                     /locus_tag="VF995_10510"
     CDS             2128..4698
                     /locus_tag="VF995_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020118197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9344033.1"
                     /translation="MLKFSLKALWAHKRRLAGTSLAVLLGVALLTGTLVLSDTLRRSI
                     DQFFVTADQGTDVVVRNATNVSDSAGGPRDLIDQGLTDQIRQLPGVAVAEPAVVGFGE
                     LIGHDGKAVSGQGPRQAGSWVPDPQLNPYRLVEGRAPQADDEVVINRGAAKDGGLHAG
                     DTTDLLTPDRVRVRIVGIATFGTADAFGGTSFTAFTLHGAQLHVTKRPNQVSTISVRA
                     TPGTRQEDLLGRVRGVLPPGTEAITGTQLTQENMAAVNSAFLEVFRNFLVVFAAVALL
                     VAAFSIYNTLSILVAQRTRESALLRALGAERGQVLGAVVVEALLIGVVAGVAGIGAGL
                     GIAAALKGIFVGFGMPMASTSLVLTGTTVAVSLTAGVAVPLLAGVIPALRASRVAPLA
                     AFRDSLAEPGVGQRGAVGLRRRAVAGLALSGAGVAVILSAVAASSTATGGGVTMRAGL
                     GALLCLAGVVVLGPVVARPVSRLLGAPLPRLRGVTGALARQNAMRNPRRTAGTAAALM
                     VGVGVVTLFTVFAASLKASTADNVAGSFHGDLVVSPGGFGGNEMSPRLAADAAKLPEV
                     RSATGMGGGTALIGGASQQAGAADPAALGDVLQLRVTQGGLRELGGKGLAISQKAADD
                     QGWRLGSTVPVTFSDGATGDFTVAAVYQARDVVGDYLMARSAWAPHAAQNLDRDVFVV
                     LRDGVDLAAGKAAVERVAARYGAPPVMDRGEYIDSLTGYVNAMLGVVYAMLALAIVIA
                     LMGIANTLSLSIHERTRELGLLRAVGQTRGQLRAMVRWESVIVALFGTVGGIGLGGFL
                     GWGLVRATAGQSDLTVFSAPLGKVLIVVVVGALAGVLAGLRPARRAARLNPLQAIASE
                     "
     gene            complement(4763..5758)
                     /locus_tag="VF995_10515"
     CDS             complement(4763..5758)
                     /locus_tag="VF995_10515"
                     /inference="COORDINATES: protein motif:HMM:NF020694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HEX9344034.1"
                     /translation="MAGVGVGVLHPGEMGSFVAAAARVGGGRAEVRWASAGRSTATRR
                     RAEADGLVDAGSVAALAAQSEIILSVCPPHAALEVARTVAGLGFGGVYLDANAVAPAT
                     TRAIGALVEAGGARFVDGGLIGNPARGLLSPRDHGSPPGRAHAAADASQPPRLYLSGP
                     AIEVARVAALFANSPLAARTVAGGAGAASALKMCYAAWTKGSAALLLAIRATATAEGV
                     DEALLAEWADSQPDAPARSTSALRGTPRKAWRWVAEMEEIAATLEAAGLPGGFHHAAA
                     EVYRRLERHKDAASPPAAEAVLADLLAAATATAMGMGMGMGAEPAPAGQRAPGSG"
     gene            complement(5764..>6624)
                     /locus_tag="VF995_10520"
     CDS             complement(5764..>6624)
                     /locus_tag="VF995_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344035.1"
                     /translation="GQGDYLVIPTSTTWRLLPGGGEAAPLRLLVIEATGSPGHIRPPR
                     RYLSEVGQFLEHSPYCERDLHGPDAPLLAEGVGADPAADTEMLVRHRAGLTRYTYAHH
                     PFDVVGWDGCLYPFRFSINDFEPITGRIHQPPPVHQTFQGPNFVVCSFVPRLFDYHPK
                     AIPVPYNHANVDSDEVIFYTGGDFMSRAGSGIAQGSISLHPSGFTHGPQPGSVEAALG
                     KPATEELAVMVDTFRPLSLGPGARDGEEPAYAWTWARRSSMQASEARPSMGDRRSRRG
                     QGPGPGPTTG"
BASE COUNT          875 a         2434 c         2428 g          887 t
ORIGIN      
        1 cctcgatgac ctggtcgccg gcgccaacgc cgccgggatc accgtcagga ccaggattga
       61 gggcgaccct cgccggctgc ccgccggggt tgatctcgcc gccttccgga tcgtccagga
      121 ggcgctgacc aacgtggccc gccacgccgg gccggccagc gcgaccgtca ccatccgcta
      181 tcagggacgg gagcttgccg tgcaggtcga cgacgacggc cgcggggccc cgccggacca
      241 gcggcgccac cacgccggcc cggctccggg cggcggcaac gggatccgcg ggatgcgcga
      301 acgcgccgag gcgctgggcg ggcagctggc cgcggagaac cggccgggcg gcgggttccg
      361 ggtggaggcc atgctgccac tcgacggcac cggcccggag ggcggaatcg atcccgccag
      421 cgacacgcct gatgccgccg gcgagcaacg ggaggaggcc gcggagcggg gtgtgccatg
      481 gtgatcaggg tcctgctcgc cgacgaccag gcgctggtcc gtgcggggtt ccgggcgctg
      541 cttgacgccc agccggatat cgaggtggtc ggcgaggccg gcgacggcgc cgccgcggtc
      601 gagctggcca gcacggccac ccccgacgta gtgctgatgg acatccgcat gccggtcctg
      661 gacggcctcg ccgcgaccca gaagatcgcc gccgacccgc gccttggcgg ggtacggatc
      721 atcatcctca ccaccttcga gctcgacgaa tacgtcttcg aggcgatccg catcggcgcc
      781 agcggcttcc tggtcaagga caccgaacca gccgacctgc tccgcggggt gcgggcggtc
      841 gccgccggcg acgcgctgct gtcgcccagc gtcaccaggc ggctgatcga gcagttcgcc
      901 acgcgcgcca agcggccccg cgacaccggg cgcctcaatg ccctcaccga ccgggaacgg
      961 gaggtgctgg cgctggtcgc cggtggcctg tccaacgacg agatcgccga gcgcctggtg
     1021 ctcagccccc tcacggtcaa gacccacgtg agccggacca tcggcaagct cgacatccgc
     1081 gaccgggccc agctggttgt cctcgcctac gagactggcc tggtccggcc cggctggctc
     1141 ggctgatccc aactcccgca gcgcccccgg accgtgccag cccgctccat ccctaccacc
     1201 acaggggtag cgcggcaccc cgggactgct cccaacaggg tagccacagt gccaccgccg
     1261 agccgacgac agcgcggcgc cgggccgcga ccatcggcga ggcagatcat cccgagtgga
     1321 aaggcgatga gcgcggccat gctcatgacc accctcccgg caaccttcac cgaccgacag
     1381 accgctcccg ccacggtggc ggcacacacg gtcagcgcca ccaagaccta cgggatcggc
     1441 gacaccgcgg tccgcgccct ggacaacgtc accgtcgcgt tcccgagcgg gcggttcacc
     1501 gccatcatgg gaccctcggg gtcgggcaag tcgaccctgc tgcactgcgc tgccggcctc
     1561 gacgatctca ccgccggcca ggcggtcatc ggcgggatcg agctcggctc cctctccgac
     1621 aagcagctca cccgcctgcg ccgcgagcag gtcggcttcg tgttccagtc gttcaacctg
     1681 ctgcccacgc tgacggcgct ggagaacatc accctcccgg cggcgctggc cgggaccaag
     1741 ccggagcagg actggctcga cgtggtgatc gacaccgtgg gactgcgtga gcggctctcg
     1801 cacctgccag cggagctgtc tggcgggcag cagcagcgag tcgccgtggc acgggcgctg
     1861 gtacgccagc cgcggatcat cttcgcggac gagccgaccg gcaatctcga ctcccgcgcc
     1921 agcgcggaga tcctcaactt cctgcgtcgc gccgtgcggg agctggacca gacaatcgtg
     1981 atggtcaccc atgacccggt cgccgccagc tacgccgacc aggcgctgtt tctggccgac
     2041 ggccggctgg tcggccgtat ggaggcaccc accgcgcagc gagtcctcga ccgcatcaag
     2101 gaagccgggg agtgaggtgc gaccgccatg ctgaagttca gcctgaaagc cctctgggcc
     2161 cacaagcgcc ggctcgccgg cacctccctc gcggtcctgc tcggagtggc gctgctgacc
     2221 gggacgctgg tgctgtccga cacgctgcgg cgcagcatcg accagttctt cgtcaccgcc
     2281 gaccagggca ccgacgtggt ggtccgcaac gccaccaacg tcagcgacag cgccgggggg
     2341 ccccgtgacc tgattgacca gggtctgacc gaccagatcc gccagctgcc cggcgtggcc
     2401 gtcgccgaac cggcggtggt cggcttcggc gagctcatcg gccacgacgg caaggccgtc
     2461 agcggccagg gtccacgcca ggccggcagc tgggtccccg acccgcagct caacccctac
     2521 cgcctggtcg agggccgcgc accccaagct gacgacgagg tggtcatcaa ccgcggcgct
     2581 gccaaggacg gcggcctgca cgccggcgac accaccgacc tgctgacgcc ggaccgggtc
     2641 cgggtgcgga tcgtcggcat cgccaccttc ggcaccgccg acgccttcgg cgggacctcc
     2701 ttcaccgcct tcacgctgca cggtgcgcag ctccacgtca ccaaacggcc caaccaggtc
     2761 tcgactatct cggtgcgggc caccccgggc actcggcagg aggacctcct cggccgtgtc
     2821 cggggggtgc tgccgcccgg gaccgaggcg atcaccggca cgcagctcac ccaggagaac
     2881 atggccgcgg tcaacagtgc ctttctggag gtgttccgca acttcctggt ggtattcgcc
     2941 gcggtcgcgc tgctggtggc ggcgttctcg atctacaaca ccctctcgat cctggtggcg
     3001 cagcggaccc gggagtcggc gctgctgcgc gcgctcggcg ccgaacgcgg gcaggtgctc
     3061 ggcgcggtcg tggtggaggc actgctgatc ggcgtggtgg ccggggtcgc cgggatcggc
     3121 gccggccttg gcatcgccgc ggccctcaag ggcatcttcg tcggcttcgg gatgccgatg
     3181 gcgtcgacca gcctggtgct caccggcacc accgtggcgg tctcgctgac cgctggggtg
     3241 gccgtccccc tgctcgccgg ggtgattccg gccctgcggg cctcgcgggt ggcgccgctg
     3301 gccgcgtttc gtgacagcct ggctgaacca ggagtcgggc agcgcggtgc tgtggggctg
     3361 cggcgccggg cggtcgccgg gctcgcgctg agcggcgccg gcgtcgcggt gatcctttcc
     3421 gcggtcgccg cgagcagtac cgccaccggc ggtggggtga ccatgcgggc cgggctcggc
     3481 gcgctgctgt gcctggccgg agtggtcgtc ctcgggccgg tcgtggcccg cccggtgagc
     3541 cggctgctcg gcgcgccgct gccacggctg cgtggcgtca ccggcgcgct cgcccggcag
     3601 aacgccatgc gcaacccccg ccgcaccgcc ggcaccgccg ccgcgctgat ggtcggcgtc
     3661 ggcgtggtca ccctgttcac ggtgttcgcc gcgtcactga aggcgtcgac cgccgacaac
     3721 gtcgccgggt ccttccatgg cgacctggtg gtgagcccgg ggggcttcgg cggcaacgag
     3781 atgagcccgc gcctggccgc cgacgccgcc aagctcccgg aggtgcgctc cgccaccggc
     3841 atgggcggcg gcaccgccct gatcggtggt gccagccagc aggccggtgc tgccgacccc
     3901 gccgcgctcg gcgacgtgct gcagttgcgg gtcacccagg gggggctgcg cgagctcggc
     3961 ggcaaggggc tcgcgatctc ccagaaggcc gctgacgacc agggctggcg gctcggcagc
     4021 accgtgccgg tgacgttcag cgacggcgcc accggcgatt tcaccgtcgc tgccgtctac
     4081 caggcccgcg acgtggtcgg cgactacctg atggctcgct cggcatgggc gccgcacgcc
     4141 gcccagaatc tcgaccggga cgtgtttgtg gtgctgcgcg acggggtcga tctggccgcc
     4201 ggcaaggcgg cggtcgaacg ggtcgcggcg cgctacggcg caccgccggt gatggaccgc
     4261 ggggagtaca tcgattcgct caccggctac gtcaacgcca tgctcggcgt cgtctacgcg
     4321 atgctggccc tggcgatcgt gatcgcgctg atgggcatcg ccaacaccct ctcgctgtcg
     4381 atccacgaac ggacccgtga acttgggctg ctccgcgcgg tcggccagac ccgcgggcag
     4441 ctgcgggcga tggttcgctg ggagtcggtg atcgtcgcgc tgttcggcac ggtcggcggg
     4501 atcggcctcg gtgggttcct cggctggggg ctggtgcggg caaccgcagg ccagtccgac
     4561 ctcaccgtct tctccgcccc actggggaag gtcctgatcg tggtggtggt tggggcactc
     4621 gccggggtgc tcgcaggcct gcgacccgcc cgccgcgcgg ccaggctgaa ccccctgcag
     4681 gcgatcgcca gcgagtgagc caagcggccg cgggcggccg agagaccgcg cggcacgcaa
     4741 cccggcctgg ccggcgcgct ccctatcccg acccgggggc gcgctggccg gccggcgccg
     4801 gctcggcgcc catgcccatg cccatgccca tggccgtggc cgtggccgct gccagcagat
     4861 cggcgagcac cgcctccgcc gctggtgggc tggcagcgtc cttgtggcgc tccaggcggc
     4921 ggtagacctc ggcggcggcg tggtggaaac cgccgggcaa tccggccgct tccagggtcg
     4981 cggcgatctc ctccatctcg gcgacccagc gccaggcttt tcggggtgtg ccgcgcagcg
     5041 cactggtgga gcgggccggt gcgtctggct gggagtcggc ccactccgcc agcagtgcct
     5101 cgtcgacccc ttcggcggtc gcagtcgccc ggatcgccag cagcagcgcg gcgctgccct
     5161 tggtccaggc ggcgtagcac atcttgagcg ccgaagcggc gcccgcgccg cccgcgaccg
     5221 tgcgggctgc caggggcgag ttggcgaaca gcgccgccac ccgcgccacc tcgatcgctg
     5281 gcccggacag gtagaggcgg ggtggctggc tggcatcggc agcggcgtga gcgcggcctg
     5341 gcggcgaacc atggtcgcgg ggcgagagca acccccgggc ggggttgccg atcaggccgc
     5401 cgtccacgaa gcgggcgcct cccgcctcca ccagagcgcc gatcgcccgg gtggtcgccg
     5461 gggcgaccgc gttggcgtcc aggtacaccc cgccgaagcc gagcccggca acggtccggg
     5521 cgacctcgag cgcggcgtgc ggcgggcaga ccgacaggat gatctcgctc tgggcggcca
     5581 gcgcggcgac actgccggcg tcgaccagcc cgtcagcctc agcgcgccgc cgggtcgccg
     5641 tgctccggcc cgccgaggcc caccgcacct ccgcgcgacc gccgcccacg cgggcagccg
     5701 cggcgacgaa cgagcccatc tcgccggggt gcaacacccc gaccccaacg ccggccatca
     5761 cggtcagccg gtcgtcggac cggggccagg gccctggccc cggcggctcc gccggtctcc
     5821 catggacggc cttgcttcgc tcgcttgcat gctcgaccgg cgggcccagg tccaggcgta
     5881 ggcgggctcc tcgccgtcgc gggcgccggg gccgaggctg agggggcgga aggtgtcgac
     5941 catgaccgcc agctcctcgg tcgccggctt gccgagggcg gcttccacgc tgccgggttg
     6001 cgggccgtgg gtgaaccccg acgggtgcag cgagatcgaa ccctgggcga tgccggagcc
     6061 ggcccggctc atgaagtcgc cgccggtgta gaagatcacc tcgtcggagt cgacgttggc
     6121 gtggttgtag ggcaccggga tcgctttggg gtggtagtcg aacaggcgcg gcacgaagct
     6181 gcagaccacg aagttcgggc cctggaaggt ctggtgcacc ggcgggggct ggtggatccg
     6241 cccggtgatc ggctcgaagt cgttgatcga gaagcggaac gggtacaggc agccgtccca
     6301 gccgaccacg tcgaacgggt ggtgggcgta ggtgtagcgg gtcaggccgg cgcggtggcg
     6361 taccagcatc tcggtgtcgg cggccgggtc ggcgccgaca ccctcggcca gcagcggcgc
     6421 gtccgggccg tgcaggtcgc gctcgcagta ggggctgtgc tccaggaact ggccgacctc
     6481 cgacaggtag cggcgcggcg ggcggatgtg gccgggcgag ccggtggcct cgatcaccag
     6541 cagtcgcagc ggcgcggcct cgccgccgcc gggcagcagc cgccaggtgg tggaggtcgg
     6601 gatcaccaga tagtcgccct ggcc
//
LOCUS       DASWDC010000327         4034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_981300, whole genome shotgun sequence.
ACCESSION   DASWDC010000327 DASWDC010000000
VERSION     DASWDC010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981300"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VF995_10525"
     CDS             complement(<1..657)
                     /locus_tag="VF995_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344036.1"
                     /translation="MSAIEPQTPSASSSKVEVPEFVASLRGYDRTQVDEWAKEMVTLL
                     RRERQRAAEAERSLRLANEDQPPPSFSHLGAHVAKIMEDAGASAENMLADAAERAEEA
                     IQAGEEEAAEVIKEAQQRAGRLEEAAQKVLENSRAEGERVKAEASEDAEETRAEADEA
                     ASAMLEEAREASDQLRNEAEADRQAIEAESNRLEALRDRALQQLGRLYGHLQSVVDEV
                     R"
     gene            1040..2230
                     /locus_tag="VF995_10530"
     CDS             1040..2230
                     /locus_tag="VF995_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344037.1"
                     /translation="MAVAVLAVATAATMLLSTEGLLPGESPAKRVGALAPWSSQLARA
                     VGDGGPRGLPTQGKKKGAAASSTRTQLDAKASGPLKPILGGLLDNARPPAESFQHGAI
                     SGFVVQVDWSELQSAPGAPLPANNPIDQAIAAARAAGLRIKLRVDAGTMAPEWAKSIG
                     GSPVTVQFPSPLTGSLTSGTIGRFWTEAFGRAYQDLQNKLAARYDNVPEIAQTSITRC
                     ITFYAEPFLRQARSVPQSARALLAAGYSDTADATCQKEEIQANTVWKHTRSGLAFNPY
                     QRLQSDGSVISDEKFTESMMRYCRGVLGPRCVLENHSIRTSGQSTLYPPMYAAIKSLG
                     GPISFQTATPAKVGDLLGAVAWAASQGACSVELPISYRTMNPAAFVPYNSRLLGVTRA
                     AAAG"
     gene            2548..2916
                     /locus_tag="VF995_10535"
     CDS             2548..2916
                     /locus_tag="VF995_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9344038.1"
                     /translation="MPDLDQVTNWIDGYVRAWNSNDPADIGALFADDATYRTEPFSAP
                     WRGRDTIVSNWLDRKDEPGQTHFTWHPLAVTPDVAIIQGETVYQQPPQTYSNLWVLRL
                     DPAGRCTDFTEWWLQHPKGQ"
     gene            2994..3758
                     /locus_tag="VF995_10540"
     CDS             2994..3758
                     /locus_tag="VF995_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9344039.1"
                     /translation="MAEARRVALVTGSSSGMGQATAKLLGEKGYDLVVTWSSREGGAR
                     ATAKAVEAAGGRALVTRCDVSDEGAVRAMLDACGAEFGRLDVLVNAGGTTTTQGPDDL
                     EATTMDAWDRIFAVNVKGVFLVTRAAAPLLREARGSVVNLASMAGVRPTGVQPHAYAA
                     SKGAVVTLTRTLAAALAPEVRVNAVAPGWVLGSWMEEQLGDNYGWLIERRARHTPLKR
                     VASNEDVAEVIVTVAEQLPLMTGALFPVDGGYTIVT"
     gene            3821..>4034
                     /locus_tag="VF995_10545"
     CDS             3821..>4034
                     /locus_tag="VF995_10545"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9344040.1"
                     /translation="MVGTAAGLAERLVELGFSHYEARAYLGLLGQQPMTGYALANATR
                     IPQPKVYETLRRLVDKRVVVQTGADPA"
BASE COUNT          604 a         1476 c         1389 g          565 t
ORIGIN      
        1 ccgcacctcg tccaccaccg actggaggtg gccgtagagc cgcccaagct gctgcagggc
       61 ccggtcgcgg agcgcctcca gccggttgga ctcggcctcg atcgcctggc ggtcggcctc
      121 ggcctcgttg cggagctggt cggaggcctc gcgcgcctcc tcgagcatgg cgctggcggc
      181 ctcgtcggcc tccgcgcggg tctcctcggc gtcctcgctg gcctcggcct tgacgcgctc
      241 gccctcggca cgggagttct ccaggacctt ctgggcggcc tcttcgaggc ggccggcgcg
      301 ctgctgcgcc tccttgatga cttccgccgc ctcttcctca ccggcctgga tggcttcctc
      361 ggcccgctcg gccgcgtcgg cgagcatgtt ctcggcggac gcgccagcgt cctccatgat
      421 cttggccacg tgggcgccga ggtggctgaa cgagggcggc ggctggtcct cgttggcgag
      481 gcggagggag cgctcggcct cggcggcgcg ctgccgctcc cgccgcagca gggtgaccat
      541 ctctttggcc cattcgtcca cctgcgtgcg gtcatagccg cgcagcgaag cgacgaattc
      601 gggcacctcg accttggagg aggacgcgga cggggtctgc ggctcgatag cgctcacggg
      661 ggacctccat atggggcgag ccgagcgggg tatgactgtg acaaccggtg gcgacacgcg
      721 cccaggatcc gagacgaacc agggcacgat cacacgagcg gctgattcta cgaccccgcg
      781 caagaggccg catgtcccac acaacgtggc gggcgccgca gccggcggcg ccgcccccta
      841 tgcgttgaag ttgaccagat cggaacgatt ctgaaatggt tcccgcagat ctgcgccccc
      901 gtcggttctt gctgatcact ccttggtgac gtccatgggc acttccactc ggcaacgcct
      961 ccccgagcat ggccgtgccg cgcgccatca gcggcaacgg ccgaagaccg ggccaacgcc
     1021 gcgccggagg ctcgtcgcgt tggccgtcgc ggtgctggcg gtcgccacgg ccgccaccat
     1081 gctgctctcc accgagggcc tgctgccggg cgagtccccc gccaagcgcg tcggcgcgct
     1141 ggcgccgtgg tcgagccagc tcgccagggc ggtcggcgac ggcggccccc gcggcctgcc
     1201 cacccagggc aagaagaagg gggccgcggc ctccagtacc aggacgcagc ttgacgccaa
     1261 ggcgtcggga cccctgaagc cgatcctcgg cgggctgctc gacaacgcca ggccgcccgc
     1321 cgagagcttc cagcatggcg cgatcagcgg cttcgtcgtg caggtcgact ggtcggagct
     1381 gcagtcggcg ccgggcgcgc cgctgcccgc caacaacccg atcgaccagg ccatcgccgc
     1441 cgcgcgcgcg gccgggctgc gcatcaagct gcgggtcgac gccgggacca tggcgccgga
     1501 gtgggccaag agcatcggcg gctccccggt gaccgtccag ttccccagcc cgctcaccgg
     1561 ctcgctgacg agcggcacca tcggccgctt ctggaccgag gcgttcggcc gcgcctacca
     1621 ggatctccag aacaagctcg cggcccgcta cgacaacgtg ccggagatcg cccagacctc
     1681 gatcacccgc tgcatcacct tctacgcgga gccgttcctg cgccaggccc gctcggtgcc
     1741 gcagagcgcc cgcgcgctgc tcgccgccgg ctacagcgac accgccgacg cgacctgcca
     1801 gaaggaggag atccaggcga acacggtgtg gaagcacacc cgctcgggcc tcgccttcaa
     1861 cccctaccag cggctgcagt cagatggcag cgtgatctcc gacgagaagt tcaccgagtc
     1921 gatgatgcga tactgccgcg gcgtgctcgg cccccgctgc gtgctggaga accactcgat
     1981 ccgcacctcc ggccagagca cgctgtatcc gccgatgtac gccgcgatca agtcgctcgg
     2041 cgggcccatc agcttccaga ccgccacccc ggccaaagtg ggcgacctcc tcggcgcggt
     2101 cgcctgggcc gccagccagg gcgcgtgctc ggtcgagctg ccgatttcgt atcggaccat
     2161 gaacccggcg gccttcgtgc cctacaacag ccgcctgctg ggcgtcaccc gcgcggccgc
     2221 cgccgggtag ccaacgtcgc cagcagcgag gccggtccgg ccttcagctc acctgctcgc
     2281 atgcgtcgcc acggagcgcc ccctggtcgc gggcgccggt cggcgacggt tggccctggc
     2341 ccggcgccgg acagcctggg agtcacccgg ctgcgcctcg ctcgcggccc gttccctcca
     2401 cgtccgcccg ccgaactcgt cccagagtgt catcgccagc acctccatac tggaaggagt
     2461 gccttctccc agatctgata cgacaaacga ctcccgctgg ttcaaggact catcggcccg
     2521 cgaggccgcg agactggagg cgaacgcatg cctgacctgg atcaggtcac gaactggatc
     2581 gacggctacg tgcgggcctg gaactccaac gaccccgccg acatcggcgc cctgttcgcc
     2641 gacgacgcca cctaccgcac cgagccgttc agcgccccat ggcgcggtcg cgacacgatc
     2701 gtgtccaact ggctcgaccg caaggacgaa cccggccaga cccacttcac ctggcacccc
     2761 ctcgccgtca cccccgacgt cgcgatcatc cagggcgaga cggtctacca gcagccaccc
     2821 cagacctaca gcaacctctg ggtcctccgc ctcgaccccg ccggccgctg caccgacttc
     2881 accgaatggt ggctgcagca ccccaagggg cagtaggctt ccggatctcc tatctactat
     2941 ggtaggtggt agcaccccgg gcaatgaatg gacgaaatag gaggaacccc ggcatggccg
     3001 aggcacggcg ggtggcgctg gtgacggggt cgtcgagtgg gatggggcag gccaccgcca
     3061 agctgctcgg cgagaaaggc tatgacctgg tggtcacctg gagcagccgg gagggcgggg
     3121 cgcgcgcgac cgccaaggcg gtggaggccg ctggcggccg ggcgctggtg acgcggtgcg
     3181 acgtcagtga cgagggcgcg gtgcgggcga tgctggacgc ctgcggggcc gagttcgggc
     3241 ggctggacgt gctggtcaac gctggcggga ccaccacaac gcaggggccg gacgacctgg
     3301 aggcgaccac catggacgcc tgggatcgca tcttcgcggt gaacgtcaag ggcgtgttcc
     3361 tggtgacccg ggcggccgcg ccgctgctgc gcgaggcgcg ggggagcgtg gtgaacctcg
     3421 ccagcatggc gggggtccgg ccgacagggg tgcagccgca cgcctacgcg gccagcaagg
     3481 gcgcggtggt caccctgacc aggacgctgg ccgccgccct cgcgccggag gtccgcgtca
     3541 acgcggtggc gcccggctgg gtgctcggga gctggatgga ggagcagctc ggcgacaact
     3601 acggctggct catcgagcgc cgcgccaggc acacccccct caagcgggtc gccagcaacg
     3661 aggacgtcgc cgaggtgatc gtcaccgtcg ccgagcagct tcccctcatg accggcgcgc
     3721 tgttcccggt cgacggcggc tacacgatcg tcacctgagc ggtgctaccg ttatcgtacc
     3781 tacgctggta acaaggatct gtgaatcgag ggaggcagcg gtggtgggca cggcggccgg
     3841 gctggccgag cgactggtcg agctcggctt ctcgcactac gaggccaggg cctatctcgg
     3901 cctgctcggg cagcagccga tgaccggcta cgcgcttgcc aacgcgacca ggatcccgca
     3961 gcccaaggtc tacgagacgc tgcgccggct ggtcgacaag cgggtggtgg tgcagaccgg
     4021 cgccgacccc gcgc
//
LOCUS       DASWDC010000328         3796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_399605, whole genome shotgun sequence.
ACCESSION   DASWDC010000328 DASWDC010000000
VERSION     DASWDC010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399605"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..790
                     /locus_tag="VF995_10550"
     CDS             185..790
                     /locus_tag="VF995_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344041.1"
                     /translation="MNAEATVLRERTRTRTRLGGASARTATLPGAPSGGVAFATQVMA
                     VPLRAVARPAGRRLSVAAALSLVLVALVSGLYDHADGGAGTTPTAARLPAPSAALAAR
                     PASALASTLGGSGASASASRPAAPARAPATASGASSADAAAVAWYAAQRHVPVSRVQA
                     LQHRQVNATAVEVMVTADTGPTGIYSDMVTVQRVGSTWKVP"
     gene            787..1881
                     /locus_tag="VF995_10555"
     CDS             787..1881
                     /locus_tag="VF995_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344042.1"
                     /translation="MTPAAKSKVFGVLAALVVLAGIAGRASPGFAVGKVTGVVSRTVR
                     HALAAGSRPTPAIQVPAGRAIGASPYALSDIPASYLALYVKAAPTCPALTWQMLAGIG
                     KVETNHGRSTLPGVHTGINSAGCCAGPMQFNINNGPPSTWQSFGRGGNVYDPADAIPA
                     AARKLCANGLALPPPAADPCRTVMGSSSVHAAIMRYNNACWYVHEVLTFAARYTSAAL
                     PPASTDPFVLALVHNPNISTTRSRGCDPGPDLASGKLDLRVQSILAVLAQRWRLRVSC
                     VKTGHSYDVAGTTRVSNHTVWRAVDIDAINGQAVTHASAASRALVLFLDRLQGPLRPT
                     EVGSPFAVGHRPYFTDEGHQEHVHIGYGAL"
     gene            complement(1908..3194)
                     /gene="hemL"
                     /locus_tag="VF995_10560"
     CDS             complement(1908..3194)
                     /gene="hemL"
                     /locus_tag="VF995_10560"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX9344043.1"
                     /translation="MNTDRSEVLFERAKARIPGGVNSPVRAFGAVGGTPRFFARGQGP
                     WLWDADGNRYVDLVLSWGPLIAGHAHPRVVEAVRAATARGTSFGAPTEAEVELAEEVA
                     RRVPAAGMLRLVSSGTEATMSAVRLARAATGRAKLVKFAGCYHGHADGLLAAAGSGVA
                     TFGLPDSPGVTAAQTADTVVLPYNDTSQFAKLFAEAGDQIAGVIVEPVAANMGVVPPD
                     PAFLAALRAETRRAGALLIVDEVLTGFRVHRAGATGLYGLEPDLLTFGKVIGGGLPAA
                     GYGGRAELLRMIAPEGPVYQAGTLSGNPLATAAGLATLALLDDVAYDHLDRLASILSS
                     GLQAAFDDAGLPARVQRAGTLFSVFLTRNEVLDYGGAVQQDTAAYARFFHAMLDRGVY
                     LPPSAYEAWFLSIAHGDAEVDQILTAARESARVAAA"
     gene            complement(3222..>3796)
                     /locus_tag="VF995_10565"
     CDS             complement(3222..>3796)
                     /locus_tag="VF995_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9344044.1"
                     /translation="LDQSLRRLGSEPIDLWQLHHPSMAAIESDELFGFLDDQVATGKI
                     RAYGVALGPGPGYGDEGAAAIGERAVAAAQLRWSLFEQDPGRELAPLAAERGASLVAR
                     MRLPPADDPRLGHLDFLTRDREQTIGQAMVKFLLRQPAVASVMPAAADVAQLRELAAA
                     VEGPGLTAEDLDMIAERYEEGFGLPGSSPI"
BASE COUNT          547 a         1434 c         1335 g          480 t
ORIGIN      
        1 gaattcaacg ccgaaatgga actggaacag gaaacgcgac cggaactgga gctggaacgg
       61 aaacgcggcg cggaaccagg agcgcgacca gaaacgcggc gcggaccgga actcgcaccg
      121 gaacccggac cggaacccgg acgcagccag ggactcgaac tcgaacctga aggagcgacg
      181 cctcatgaac gcagaagcaa ccgtccttcg cgaacgaacc cgcacccgga cccggcttgg
      241 cggcgcctcg gcccgcaccg ccacgctgcc cggcgcgccc agtggtggcg tggccttcgc
      301 cacccaggtc atggccgtac cactgcgcgc cgtcgccagg cccgccgggc gccgcctgag
      361 cgtcgccgcg gcgctgtcac tcgtgctggt cgcgctggtg agcgggctct acgaccacgc
      421 cgacggcggc gcgggcacca ccccgacggc cgcccgcctc ccggccccat cggcggcgct
      481 cgcggccagg ccggccagcg ccctcgcctc caccctgggc ggcagtggcg ccagcgccag
      541 cgcgagccgg cccgccgctc cggcccgggc cccggcgacc gcgagcggtg cctcgtcggc
      601 cgacgccgcc gccgtcgcct ggtacgccgc ccagcgccac gtcccggtca gccgggtaca
      661 ggcgctgcag caccggcagg tgaacgccac cgcggtcgaa gtcatggtga cggccgacac
      721 tggcccgact ggaatttact ccgacatggt gacagtccag cgtgtcggat cgacctggaa
      781 ggtgccgtga cgcctgcggc caagtcgaag gtgttcggtg tcctcgccgc cctggtcgtc
      841 ctcgccggga tcgccgggcg cgcctccccg ggcttcgcgg tcggcaaggt taccggggtg
      901 gtcagccgga ccgtccgcca cgcgctggcc gccggttcgc gccccacccc agcgatccag
      961 gtcccggcgg gccgcgccat cggcgcgagc ccgtatgccc tctccgacat cccggcgagc
     1021 taccttgccc tgtacgtcaa ggcagccccg acctgcccgg cgctcacatg gcagatgctc
     1081 gccgggatcg gcaaggtcga gaccaaccac ggccgctcga ccctgcccgg cgtccatacc
     1141 ggcatcaact cggcgggctg ctgcgccggt ccgatgcagt tcaacatcaa caacggaccc
     1201 ccctccacct ggcagagctt cggccgcggc ggaaatgtct acgaccccgc cgacgcgatc
     1261 ccggcggcag cccgcaagct gtgcgccaac ggcctcgccc tgccgccgcc agccgccgac
     1321 ccctgccgaa cggtgatggg cagctcatca gtccacgcgg cgatcatgcg ctacaacaat
     1381 gcctgctggt acgtgcacga ggtgctgacc ttcgccgccc gctacacctc cgccgccctc
     1441 cctccggcct cgacagaccc cttcgtcctg gcgctggtcc acaatccgaa catctcgacg
     1501 accaggagcc gcggctgcga ccccggcccg gacctcgcca gcggcaagct ggacctgcgg
     1561 gtccagtcga tcctggcggt gctcgcccag cgctggcggc tgcgggtctc ctgcgtcaag
     1621 accggccaca gctacgatgt ggccgggacg acccgggtct caaaccacac ggtgtggcgc
     1681 gcggtcgaca tcgacgcgat caacggccag gcagtcaccc acgccagcgc ggcctcccgt
     1741 gccctggtcc tgttcctcga ccgcctccag gggccgctgc gccccaccga agtcggctcg
     1801 ccgttcgcgg tcggccaccg tccctacttc accgacgagg gccaccagga gcacgtccac
     1861 atcggctacg gcgccctgta gccctgtagc cgatgtggag ctccggatca ggccgcggcg
     1921 acgcgggcgg actcgcgggc ggcggtgagg atctggtcga cctcagcgtc gccgtgggcg
     1981 atcgagagga accaggcctc gtaggcgctt gggggcaagt agacgccgcg gtcgagcatg
     2041 gcgtggaaga agcgggcgta ggcggcggtg tcctgctgga cggcgccgcc gtagtcgagg
     2101 acctcgttcc tggtgaggaa gacgctgaag agagtgccgg cgcgctgcac cctggccggc
     2161 agcccggcgt cgtcgaaggc agcctggagg ccgctgctga ggatgctggc gagccggtcg
     2221 agatggtcgt aggcgacatc atcgagcagg gcgagggtgg cgaggcccgc cgcggtcgcc
     2281 agcgggttcc ctgagagggt gccggcctgg tagaccggcc cctccggggc gatcatgcgc
     2341 agcagttcgg cgcggccgcc gtagccggcg gcaggcaggc cgccgccgat caccttgccg
     2401 aaggtgagca ggtcgggctc caggccgtag aggcccgtcg cgccagcccg gtgcacgcgg
     2461 aagccagtca ggacctcgtc gacgatcagc agcgcgccgg cacggcgggt ctccgcgcgg
     2521 agggccgcca ggaacgccgg gtcaggcggg accacgccca tgttggcggc gaccggctcg
     2581 acgatcaccc ccgcgatctg gtcgccggcc tcggcgaaca gcttggcgaa ctggctggtg
     2641 tcgttgtagg gcagcaccac ggtgtcggcg gtctgggcgg cggtcacccc cggcgagtcg
     2701 ggcaggccga aggtggccac gcccgaaccg gcggcggcga gcagcccgtc ggcgtggccg
     2761 tggtagcagc cggcgaactt gaccagcttg gcacggccgg tggcggccct ggccagccgg
     2821 accgcgctca tggtcgcctc ggtgccggag ctgaccaggc ggagcatccc ggcggcgggg
     2881 acgcggcgag cgacctcctc ggccagctcg acctcagcct cggtgggtgc cccgaacgag
     2941 gtcccccggg cggtcgccgc ccgcaccgcc tcgaccaccc tggggtgggc gtggccggcg
     3001 atcagcgggc cccaggagag caccaggtcg acgtagcggt tgccgtccgc gtcccacagc
     3061 cacggtccct ggccgcgcgc gaagaagcgc ggcgtcccgc ccaccgcgcc gaaggcccgc
     3121 accggcgagt tgaccccgcc ggggatgcgc gccttggccc gctcgaacag cacctcgctg
     3181 cgatcagtgt tcatcgtgct ggtcgtctcc tggttcgctc gctagatcgg actcgagccc
     3241 ggaagcccga agccttcctc gtagcgttcg gcgatcatgt cgaggtcctc ggcggtcagg
     3301 ccgggcccct ccaccgccgc cgccagctcc ctgagctgcg ccacgtcggc ggcggccggc
     3361 atgacgctcg cgacggccgg ctgccgcagg aggaacttga ccatcgcctg gccgatcgtc
     3421 tgctcgcggt ccctggtgag gaagtcgagg tggcccaggc gcggatcgtc cgccggcggc
     3481 agccgcatgc gcgcgaccag cgaagcgcca cgctcggcgg cgagtggtgc cagctcgcgc
     3541 ccggggtcct gctcgaacag cgaccagcgc agctgcgccg cggccacggc acgttctccg
     3601 atcgcggccg cgccctcgtc cccatacccc gggcctgggc cgagcgccac gccgtaggcg
     3661 cggatcttgc cggtggcgac ctggtcgtcg aggaagccga acagctcgtc ggactcgatc
     3721 gcggccatgc tcggatgatg cagctgccac aggtcgatcg gctcgctccc aaggcgccgc
     3781 agcgactggt ccagcg
//
LOCUS       DASWDC010000329         4197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_146291, whole genome shotgun sequence.
ACCESSION   DASWDC010000329 DASWDC010000000
VERSION     DASWDC010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146291"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..688
                     /locus_tag="VF995_10570"
     CDS             416..688
                     /locus_tag="VF995_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344045.1"
                     /translation="MTVPVVISALAGAVVGAACMAFWAAERIQAEAARQFDHGFERGR
                     WLSELAAVRALERAGGEHGRTRRSARLSESTRAVGISQALTARRRG"
     gene            complement(654..1481)
                     /locus_tag="VF995_10575"
     CDS             complement(654..1481)
                     /locus_tag="VF995_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344046.1"
                     /translation="MTTGKLSLAMSRVDRRVAEQRLGAWGTISTRDARWQLGLLPWQE
                     AAPLGSHRYLNGWVIGTSGSQILRPGDDPSKLWESPGYQADRQLFQAAAGDLTALKMD
                     GCIGVDDWLVKHIDPGTRRVMERWPDGMPHSWRLQDHGNMLRVPYGGVVAEARPRLEA
                     TMREVADRNDLQFGHFRNARAYELYGTQVVKNKAAAVARLMDETELRHVVTFEDTDRT
                     LADLARSEASDFTLVTVVSPEMTPDRRAALEAAHFYAKSPRQVARTLTLVAARSELG"
     gene            complement(1900..2784)
                     /locus_tag="VF995_10580"
     CDS             complement(1900..2784)
                     /locus_tag="VF995_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344047.1"
                     /translation="MSWHLGWWAPLPPGEDPDDLLDPPVHGPLLSAAAGGAPLDRLGQ
                     NRLAGLKAAGLASPEGRPRFPVARAEQAAVLRAAAADLGSSIARLVAGEWSRLEVEYE
                     PVHDTVSGTGGPSGPGGPAAAFLVVGGLVLDVGVRRLLRLQGLAAPPFGSAFVWLAEG
                     DGPQAAGLWAARVTNLPARGSLVRFGRPSAPRWELAAVDPEAAPALSAAQEPGLRELC
                     EALGWSVVKLIESALPAFDPIRRRIPGADDEGAFLAWAYRLAVDAALDSLAARGLLAP
                     PIDGVVAIRVSDPALAGM"
     gene            complement(2824..3537)
                     /gene="rplA"
                     /locus_tag="VF995_10585"
     CDS             complement(2824..3537)
                     /gene="rplA"
                     /locus_tag="VF995_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX9344048.1"
                     /translation="MAGKKFAEAANKVDRSKFYGPLEALQLAKETNPTKFDATVEVAF
                     RLGVDPRRADQMVRGTVSLPNGTGKTIRVAVFAEGEAARAAEEAGADVVGGTELVQRV
                     QGGFLDFDAAVAVPEMMGQVGRLGRILGPRGLMPNPRAGTVTPDVAKAVTDIKGGKVE
                     YRVDRHGNLHLILGKTSFETHALSENYQAVLDEVLRAKPAAAKGRYIKGITVATSMGP
                     GIKVDAAQTRNVTGEAGPA"
     gene            complement(3646..4089)
                     /gene="rplK"
                     /locus_tag="VF995_10590"
     CDS             complement(3646..4089)
                     /gene="rplK"
                     /locus_tag="VF995_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX9344049.1"
                     /translation="MPPQTTRRRRVTAVIKIELPAGQATPAPPVGTALGPHAVNIMDF
                     VKQYNAATQAQIGNVIPVEITIYEDRTFSFVLKTPPAAVLLRKAAGVDKGSPEPHKTK
                     VGTVTRDQVREIAETKMPDLNAVDIEGAMKIVEGTARSMGITVAS"
     gene            complement(4125..>4197)
                     /locus_tag="VF995_10595"
     CDS             complement(4125..>4197)
                     /locus_tag="VF995_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX9344050.1"
                     /translation="KVLVSIFGRETPVELAFDQVQKL"
BASE COUNT          565 a         1471 c         1517 g          644 t
ORIGIN      
        1 cgaggcgcgc agctcgccca gttccttgac cttcagcccc cggtactcgg tgagcaccgc
       61 ggccaccgag ccttcgagat accccttgat ggcctcgacc tcggcgacgt tgctcgcgct
      121 cggcacggcc tcacctcccg acaaccaaca cacgaaaaac ggacggtcag ccgaccgtcc
      181 gcttggcatg cggcgccgtg aagcgagcgc tgctctttgc ctgcgccggc gccctcgtgc
      241 agtgactcgg gccttcgtgc cgcatggcaa ccggacggtc ttcggctcgg gcgagggtac
      301 ttggcaacca cgggcggaag caagtaggca agcctgtgga taacttcagc agcgattgct
      361 tggttttgct tatgatctcc tcagttagca accgaaccgg atcaaggggg acccgatgac
      421 cgtacccgtc gtcatcagcg cgcttgctgg cgccgtggtc ggcgctgcct gcatggcctt
      481 ctgggccgcc gagcggatcc aggcggaggc cgcccgccag ttcgaccacg gcttcgagcg
      541 cggccgctgg ctctccgagc tggccgctgt ccgcgcgctg gagagggctg gcggtgagca
      601 cgggcgcacc cgtcgaagcg cccgcctcag tgaatccacc cgcgccgtgg ggatcagcca
      661 agctctgacc gcgcggcgac gagggtgagg gtcctggcga cctggcgcgg gctcttggcg
      721 tagaagtgcg ccgcttccag ggcggcgcgc cggtccggcg tcatctctgg cgagacgacc
      781 gtgaccaggg tgaagtcgga ggcctcgctc ctggccaggt cggcgagcgt ccggtcggtg
      841 tcctcgaagg tgacgacatg gcggagttcg gtttcgtcca tgagccgcgc aaccgccgcc
      901 gccttgttct tcacaacttg ggtgccgtac agctcgtagg cgcgggcgtt gcggaagtgc
      961 ccgaattgca ggtcgttacg gtcggcgacc tcccgcatcg tggcctcaag ccgcggtcgt
     1021 gcctcagcga cgacgccacc gtaggggact cgcagcatgt tgccgtgatc ctgcagccgc
     1081 cagctgtgcg gcatgccgtc gggccaccgt tccatgaccc gccgggtccc tgggtcaatg
     1141 tgcttcacga gccagtcgtc gaccccgatg cacccgtcca tcttcagggc cgtgaggtcg
     1201 ccggccgccg cctggaacag ctgccggtca gcctgatagc ccgggctctc ccagagcttg
     1261 ctcgggtcgt ccccgggacg aaggatctgg cttccggagg tcccgatgac ccacccgttc
     1321 aggtatcggt gcgagcccaa cggcgcggcc tcctgccagg gcagcagacc gagctgccag
     1381 cgggcgtctc gcgtcgagat ggtgccccag gccccaagcc gctgctccgc cacccgccgg
     1441 tcgacccggc tcatcgccag ggacagcttc cctgtggtca cctccccggt ccccgcggaa
     1501 aggcagacac cgtcaaggtc ggtgaaccat gctgattccg gcaggggccc gacccgctcc
     1561 agcagcgatt cggagctcca ctccacactg cgagtcccaa gctccttgag caaccgcttg
     1621 gcatcgtgga aggtgtcggg gccgggacca tagcctggcc aatggatcca gccctggtcc
     1681 tgaaggggaa cgccgtcgcc tcggtaccag ccccgccgta ccgggccgac gacctctgtc
     1741 gacggcatcc gggtgggcgt atcgccggcc gcttccgcgc tgctcgcacc acgatcggac
     1801 acataccctc ccggaaccag tcccggaacc atggcatcgg cttccggtat ggccatgcgc
     1861 gggcacagcg gcggacgcga ccggggacca agctgcgcgc tacatccccg ccagggcggg
     1921 gtcgctgacg cggatggcga ccacgccgtc gatcggcggg gcgagcagcc cgcgggcggc
     1981 gaggctgtcg agggcggcgt ccacggccag gcggtaggcc caggcgagga aggcgccctc
     2041 gtcgtcggcg ccggggatgc ggcggcggat cgggtcgaag gcgggtaggg cgctctcgat
     2101 cagcttcacc accgaccagc cgagggcttc gcacagctcc cgcagcccag gctcctgggc
     2161 ggcggagagg gcgggcgccg cctcggggtc gacggcggcc agctcccaac ggggcgcgga
     2221 cgggcggccg aagcggacca ggctgccgcg ggcgggcagg ttggtgaccc gcgcggccca
     2281 cagcccggct gcctgggggc cgtcgccctc ggccagccag acgaaggcgc tgccgaacgg
     2341 cggcgctgcg agtccctgga ggcgcagcag gcggcggacg ccgacgtcga ggaccaggcc
     2401 gccgacaacc aggaaggccg cggccggccc gccgggacca ctgggaccgc cggtgccgga
     2461 gacggtgtcg tggaccggct cgtactcgac ctccaggcgg ctccactcgc cggccaccag
     2521 ccgggcgatc gagctgccca ggtcggcggc ggcggcacgc agcacggcgg cctgctcggc
     2581 gcgggcgacc gggaagcgcg gccggccctc cggcgaggcc agcccggccg ccttcagccc
     2641 ggcgagccga ttctggccga gacggtcgag cggggccccg ccggccgccg ccgacagcaa
     2701 cggcccgtgg accggcgggt cgagcaggtc gtccggatcc tcaccgggcg gcagcggcgc
     2761 ccaccagccc aaatgccagg acaccgcccg ccctcctccc gggtctcgag ccaggggctc
     2821 tgctcaggcg ggacccgcct cgccggtcac gttgcgggtc tgggcggcgt cgaccttgat
     2881 tcccggcccc atcgaggtgg cgacggtgat gcccttgatg tagcggccct tggcggcggc
     2941 gggcttggcc cgcagcacct cgtccagcac ggcctggtag ttctcggaca gcgcgtgggt
     3001 ttcgaacgag gtcttgccga ggatcaggtg caggttgccg tggcggtcca cccggtactc
     3061 gaccttgccg cccttgatgt cggtgactgc cttggcgacg tcgggggtga cggtgccggc
     3121 gcgcgggttc ggcatcaggc cgcgcgggcc gaggatgcgg cccagccggc cgacctggcc
     3181 catcatctcg ggcacggcca ccgcggcgtc gaagtcgagg aagccgccct gcacccgctg
     3241 gaccagctcg gtgccgccga ccacgtcggc gccggcctcc tcggcggcgc gggcggcctc
     3301 gccctcggcg aagacggcca cgcggatggt cttgccggtg ccgttgggga gcgagacggt
     3361 cccgcggacc atctggtcgg cccggcgggg gtcaaccccg agccggaagg cgacctcgac
     3421 ggtggcgtcg aacttggtcg ggttggtctc cttggcgagc tgcagggcct cgagggggcc
     3481 gtagaacttg ctgcggtcga ccttgttggc cgcctcggcg aacttcttgc cagccattct
     3541 ggcgattcct cctgtggtca gcggggcggc gtcgcgcccc tcccacgtcg ttgctgtcgt
     3601 tgctgtcgaa gcttctcgga ccgggttggt cgcgggcgcg ggggctcagc tcgcgacggt
     3661 gatgcccatc gagcgggcgg tgccctcaac gatcttcatg gcgccttcga tgtcgacggc
     3721 gttcaggtcg ggcatcttgg tctcggcgat ctcacggacc tggtcgcggg tgaccgtgcc
     3781 gaccttggtc ttgtgcggct cgggcgagcc cttgtcgaca ccggcggcct tgcgcagcag
     3841 caccgcggcg ggcggggtct tgaggacgaa actgaaggtg cggtcctcgt agatggtgat
     3901 ctcgacgggg atcacgttgc cgatctgcgc ctgcgtcgcg gcgttgtact gcttgacgaa
     3961 gtccatgatg ttgaccgcgt gcgggccgag cgcggtaccc accggcggcg cgggggtggc
     4021 ctgcccggcg ggcagctcga tcttgatcac ggccgtcacc ctgcggcgac gggttgtctg
     4081 gggtggcatc tgcggctttg gctccttggg ttcctccagc gctgctacag cttctggact
     4141 tggtcgaacg cgagctcgac gggggtctcg cggccgaaga tggagaccag caccttg
//
LOCUS       DASWDC010000330         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_146567, whole genome shotgun sequence.
ACCESSION   DASWDC010000330 DASWDC010000000
VERSION     DASWDC010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146567"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VF995_10600"
     CDS             complement(<1..570)
                     /locus_tag="VF995_10600"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX9344051.1"
                     /translation="MTRRLLLTYLTLTAFVLVVLAAPLGVLFARAERRHVADQVRHDA
                     LVLALEAGQALQTGQRADLPRLAAGYQASTGGRVTIVDRAGLVLADSDPAQPGQRNFA
                     DRPEVAAALRGQETSGQRHSTTLGRDLFYAATPMVWRGQVTGAVRIVYPPSLVNRRIV
                     ESWLLLGVVALAVLAVAALAGKLLAGSVNR"
     gene            complement(586..1272)
                     /locus_tag="VF995_10605"
     CDS             complement(586..1272)
                     /locus_tag="VF995_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014669552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9344052.1"
                     /translation="MRVLLVEDDDTIAGPLAKGLERAGFAVREVRTGTEALEAPEPDV
                     VLLDLGLPDLDGYEVCRRLRARSDVPIIVVTARSDEVDRVIGLELGADDYIVKPFGFR
                     ELIARIRAVTRRRAPQAQSEGPPGALQVQVGALTIDRRTRRVTLAREEVPLTPKEFDL
                     LALLAEDPGAVFTRQRILETVWDPHWYGPTKTLDVHVASLRRKLGGSGWIETVRGVGF
                     RLSSPSPSGR"
     gene            1443..>1645
                     /locus_tag="VF995_10610"
     CDS             1443..>1645
                     /locus_tag="VF995_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344053.1"
                     /translation="MNRKKLLAVLGALAVAATAGAIAIGANLGLFSLAGRTDPVGRLS
                     PTSQVALDAGTATSGAGGATQLKT"
BASE COUNT          225 a          607 c          602 g          211 t
ORIGIN      
        1 gcggttgacc gacccggcca gcagcttgcc ggccagcgcc gcgaccgcca ggaccgccag
       61 cgcgaccacg ccgagcagca gccagctctc gacgatgcgg cggttcacca gcgacggcgg
      121 gtagacgatc cgcaccgccc cggtgacctg cccgcgccac accatcggcg tggccgcgta
      181 gaacaggtcg cgcccgaggg tggtggagtg ccgctggccg ctggtctcct gcccgcgcag
      241 ggccgcggcg acctcgggcc ggtcggcgaa gttgcgctgg ccgggctggg ccgggtcgga
      301 gtcggccaag accaggccgg ccctgtccac gatcgtcacc cgcccgccgg tgcttgcctg
      361 gtagccggcg gcgaggcgcg gcaggtccgc gcgctggccg gtctgcagcg cctgcccggc
      421 ctccagcgcg agcaccagcg cgtcgtggcg cacctggtcg gcgacgtgac gccgctccgc
      481 ccgcgcgaac agcacgccga gcggcgcggc cagcaccacc agcacgaacg ccgtgagggt
      541 caggtaggtg agcagcagcc gccgggtcat ggcatcacct ggcggtcagc gcccgctcgg
      601 gctcgggctg ctgaggcgga agccgacgcc gcggacggtc tcgatccagc cgctgccgcc
      661 gagcttgcgg cgcagcgacg cgacgtgcac gtccagcgtc ttggtggggc cgtaccaatg
      721 gggatcccag accgtctcga ggatgcgctg gcgggtgaag accgccccgg ggtcctcggc
      781 gagcagggcg agcaggtcga actccttggg ggtcagcggc acctcctcgc gggcgagggt
      841 gacccggcgg gtgcggcggt cgatggtgag ggcgccgacc tggacctgga gcgcgccggg
      901 cggcccctcg ctctgggcct gcggggcgcg gcggcgggtc accgcgcgga tccgcgcgat
      961 cagctcgcgg aagccgaacg gcttgacgat gtagtcgtcg gcgccgagtt ccagcccgat
     1021 cacccggtcg acctcgtcgg agcgggcggt caccacgatg atcggcacgt cggagcgggc
     1081 ccgcagccgc cggcagacct cgtagccgtc caggtcggga agcccgaggt ccagcagcac
     1141 cacgtcgggc tcgggcgcct ccagcgcctc ggtgccggtg cggacctcgc ggacggcgaa
     1201 cccggcccgc tccagtccct tggcgagcgg cccggcaatc gtgtcgtcgt cctcgaccag
     1261 cagaactcgc acccggctac catggcacat tcagccccga agtacatctt tgggtcgtct
     1321 ttaccaaatt ttggcgggtc gcttccgctc ccttttgcgc cctcttggcc gtgatcgggt
     1381 agggtctccc cgaaccgggg gcaccgagca ggtcaagtcg aaactgagag gacggtgctg
     1441 ccgtgaatcg caagaagctg ctggcggtcc tgggcgccct cgccgtggcc gccacggccg
     1501 gcgcgatcgc gatcggagcc aacctgggcc tgttcagcct cgccggccgc accgacccgg
     1561 tcggccgcct cagccccacc agccaggtcg ccctcgacgc cggcaccgcg acctcgggag
     1621 cgggaggcgc cactcagctc aagac
//
LOCUS       DASWDC010000331         6978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_472261, whole genome shotgun sequence.
ACCESSION   DASWDC010000331 DASWDC010000000
VERSION     DASWDC010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472261"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VF995_10615"
     CDS             <1..229
                     /locus_tag="VF995_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344054.1"
                     /translation="QISRWIPRPYTTLGTDGFGRSDTRSALRRHFKIDAPHIAVTVLQ
                     QLALSGDLKPEAVAEAIKTYGLDPDATTEVP"
     gene            534..944
                     /locus_tag="VF995_10620"
     CDS             534..944
                     /locus_tag="VF995_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344055.1"
                     /translation="MDAQLSERHTPGTLWKETQRHWHCYADELNVGERVEYNRPEQTV
                     AKTPDEVMAWMEAETDKLLPRVTDRWRAEADRLRSNETLREIHLAALRGGKGTAAVIR
                     LSDARVMCLQAVQAGPPINHPGILGSQECVRHPS"
     gene            complement(961..2091)
                     /gene="gcvT"
                     /locus_tag="VF995_10625"
     CDS             complement(961..2091)
                     /gene="gcvT"
                     /locus_tag="VF995_10625"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX9344056.1"
                     /translation="MTPAEAEAADPLRRSPLDAVHRRLGAKMGAFAGWDMPIEYAGTL
                     SEHRAVREAVGIFDLSHLGKVEVSGPGAHDTLQHALSNDLDRLTEEGAAQYTLCLTDD
                     GGITDDLIVYREADGYLLVPNAANWPAVADAVRASSHRGDTEVTPRPDLAVIAVQGPA
                     APELLGGLFEEADSLPYMHQAPITRNGEPARLCRTGYTGERGYELIVAGDRAPALWDE
                     LFDCGDQFGAVPCGLGARDTLRLEMGYPLHGNDISTATDPFTARLGWAVRMDKPSFRG
                     KPALEDRKQSGPERLLVGLVATDRLIPRHGMPVIDPDAPDGRPIGEVTSGTFSPTLRH
                     GIALAYVVPAFAKDGIELLVDVRGRRGTARVTRPPFVDTSPK"
     gene            complement(2241..2501)
                     /locus_tag="VF995_10630"
                     /pseudo
     CDS             complement(2241..2501)
                     /locus_tag="VF995_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2572..3024)
                     /locus_tag="VF995_10635"
     CDS             complement(2572..3024)
                     /locus_tag="VF995_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9344057.1"
                     /translation="MEVSEIMTKEVVTVGPEYNVADVASMMHAQGIGSVIVLEDDRVL
                     GILTERDILKVVGSGEDPKNVAAHEALTDDLITVSPAASVQEAAREMVRAGVRHLPVV
                     SDEGLIGIVSIRDLVRWSVQGMAESTSTELPHIEVSQQVLSLVHKADT"
     gene            3207..4625
                     /gene="lpdA"
                     /locus_tag="VF995_10640"
     CDS             3207..4625
                     /gene="lpdA"
                     /locus_tag="VF995_10640"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9344058.1"
                     /translation="MPSDSYDLVILGGGTGGYSAALRGAGLGLSVALVEKDKVGGTCL
                     HRGCIPTKALLHAAELVDGIHEAADRWGVKTAIESIDYSATVANREDIVTKNFKGLEG
                     HLKKEKVDILAGNGKVTGPRQVEVEGVGTVTAGKALVLASGSVPKSLPGLEIDGQRII
                     TSDHALQLDYLPKSVIVLGAGAVGVEFASFYRSMGAGVTVVEILPGMVPLEDADIQKE
                     LGRAFRKRGIEVLVDTRFEEAKVDGDQVKATVTSKGGKGGKGDTKELTAELLLVATGR
                     GPVSAGLGYESIGVTLDRGYVVPKNWDTLETDAEGVHAVGDLLPPPSLALAHASFAEG
                     MLVAELAAGLPSPPIDYAGVARVTFSTPEVASVGLTEAQAREQGYEVVTNRFPFNGVA
                     KGMIHGQGGMVKLVAAKDGPVLGVHLVGARVTELVSEAMLVYNWEALPSDVAQFIHPH
                     PTLSEAVGEAYLTLAGRPLHQG"
     gene            4656..6149
                     /locus_tag="VF995_10645"
     CDS             4656..6149
                     /locus_tag="VF995_10645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX9344059.1"
                     /translation="MATPVKLPELGESITEGTITRWLKQEGDRVEADEPLYEVSTDKV
                     DTEVPAPVAGVLRAIKVQEDETVAVGTELAVIEQGGEADGDGAGAGAGTAQAAAEPEA
                     ETEPEAETEAQAEPEAEPAPQPKAEAEPEPEAEAAEKPQREPQPQAQAERDGGRDGRS
                     GAGAQASPLVRRLAREQGVDLSRVEGTGPGGRVKREDIDAYLGQRGEQPAAAKAKAPA
                     RAAEAPAPGAGPAPAGPAPRVAVPLAQGLREEVVRSTRLRRLIAERMVSSLQTSAQLT
                     AAVEVDMTRVMRLRERAKDQFKQREGLSLSPLPFAVKALIAAVKQYPSVNSALDEDGN
                     LHIYQDVHLGVAVDTPKGLFVPVIKGADSLNLVGLTRAIADLARRTRDGSIGPDELSG
                     STITVTNTGSRGTVWDTPIINQPNAAIFATPAIVKRPWVIDDPDLGEIIVVRHIMEAI
                     LTYDHRIIDGADAARFLGAIKQVLEGDDFTNELAAELGVKDAGKNKG"
     gene            6163..6873
                     /gene="lipB"
                     /locus_tag="VF995_10650"
     CDS             6163..6873
                     /gene="lipB"
                     /locus_tag="VF995_10650"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX9344060.1"
                     /translation="MEPLWTVRAGLVPYHRAWAWQRALVERRAAGEIPDTVLLLEHPH
                     VFTIGKRGGDADVLVSEAWLAAQGAEVVHSDRGGQVTYHGPGQLVGYPITLLEPGPGV
                     WPFIRRVEDALCDVAAEFGLAARGEGGLLTGVWVGEGAAEAKLAAIGMRVSRGVTSHG
                     FALNCDTDLSKFNAIVPCGMPDKPACSLSSLLSRQVTVAEVLPLAERHLAERLGRDAV
                     PVDLATLELPESDPAAVP"
BASE COUNT         1082 a         2335 c         2603 g          958 t
ORIGIN      
        1 ccagatctcc cgctggatcc ctcgccccta caccaccttg ggcaccgacg gcttcggccg
       61 ctccgacacc cgctccgccc tgcgccgcca cttcaagatc gacgcccccc acatcgccgt
      121 caccgtcctc cagcaactcg ccctctccgg cgacctcaag cccgaagccg tcgccgaagc
      181 catcaagacc tacggcctcg accccgacgc caccactgag gtcccctgag cttccagcca
      241 caacgcttcg tcaccacgca tgagctgaat gcttaaaatg cgtgcctcag ggctgttgct
      301 ggaatgatcc tatcctcgag cggaggcgaa tgatggccga agactgcccg aagggcggca
      361 agcacgactg ggagcccgta ggggacggac acacagtcaa atgcaagaag tgcggcaagt
      421 acggcgtcgt ttcgtgactc gcagctcggc aacctgaagg tttcgtcgcg gtcacactca
      481 gccactatcc tgctctggcg agcggagtcg atcacggagg aaggggagcg ctcttggacg
      541 cccagctttc cgaacgtcac accccaggaa cgctgtggaa ggagacacaa cggcactggc
      601 actgctacgc cgacgagctg aacgtcggtg aacgggttga gtacaaccgc cctgagcaga
      661 ccgtcgccaa gacgccggac gaggtcatgg cgtggatgga agcggagact gacaagcttc
      721 tgccgcgtgt caccgaccgg tggcgcgctg aggctgaccg gctgcgatcg aatgagacgc
      781 ttcgcgagat tcatctggcc gcccttcggg gggggaaggg aacagctgct gtcattcgcc
      841 tgtcggacgc ccgggtcatg tgcctgcagg cggttcaggc cggaccgccg atcaaccacc
      901 cgggcattct cggctcgcag gagtgcgtca ggcacccgag ctgacctgtt accggcgcgg
      961 ttacttcggc gacgtgtcga cgaacggcgg ccgggtgacc cgagcggtgc cgcgccggcc
     1021 gcggacgtcc acgagcagtt cgatgccgtc cttggcgaac gcagggacga cgtaggcgag
     1081 ggcgataccg tggcgcaggg ttggggagaa ggtgccgctg gtgacctcgc cgattgggcg
     1141 gccgtcggga gcgtcggggt cgatgacggg catgccgtgg cggggaatga ggcggtcggt
     1201 ggcgaccagg ccgacgagga ggcgctcggg gccggactgc ttgcggtcct ccagggcggg
     1261 tttgccgcgg aacgacggtt tgtccatgcg gacggcccag ccgaggcggg cggtgaaggg
     1321 gtcggtggcg gtggagatgt cgttgccgtg gagggggtaa cccatttcca ggcggagggt
     1381 gtcgcgggcg ccgaggccgc aggggaccgc gccgaactgg tcgccgcagt cgaagagttc
     1441 gtcccagagg gcgggggcgc ggtcgccagc gacgatcagt tcgtagccgc gctcgccggt
     1501 gtagccggtg cggcagaggc gggcgggctc gccgttccgg gtgatcgggg cctggtgcat
     1561 gtacgggagg ctgtcggcct cctcgaacag gccgccgagc agctcggggg cggcggggcc
     1621 ctggacggcg atcacggcga ggtcggggcg gggggtgacc tcggtgtcgc cacggtggga
     1681 tgaggcccgg accgcgtcgg cgaccgccgg ccagttggcg gcgttgggga ccagcaggta
     1741 gccgtccgct tcgcggtaga cgatcaggtc gtcggtgatg ccgccgtcgt cggtgaggca
     1801 gagggtgtac tgggccgcgc cctcctcggt gaggcggtcg aggtcgttgg agagggcgtg
     1861 ctgcagggtg tcgtgggcgc ccgggccgct gacctcgacc ttgccgaggt gggagaggtc
     1921 gaagatgccg acggcttcac ggacggcgcg gtgctcgctg agggtgccgg cgtactcgat
     1981 cggcatgtcc cagccggcga aggcgcccat cttggcgccc aggcggcggt ggacggcgtc
     2041 cagcggcgag cggcgcagcg ggtccgccgc ttcggcctcg gcgggcgtca ctcccgctcg
     2101 cccgcctcgg gagccggcga gggtgcgggc ggggccgggg gccgctgtgg ccgggggatc
     2161 cggccgggcg gctgggaggt gcggaccggc ggggcgccgg gctgctggag gcggcggtcg
     2221 gcgcggccct cggccttgag gcgggcgttg taggcacgca tccggtcggg gtagccgacg
     2281 atctgcacgt cgtagacggg gatgctcagc tcgtgggcga ggccgcgggc gaccttgggc
     2341 gaaccgatgc ggcggcgggt ccactcgccg tcggcggcga tcgccatggc ggtggtgtcg
     2401 gtgaccatcg tctccggctc cacgtagagc tcgacgccgg tgcggctgcg ggcgaactcg
     2461 cggagggcgt cgaggtcctc gggggtcgcc tcccggtcca tgctggcgac ctgggtgccc
     2521 cggccgcggc ggatgcggag tgcgtccagg aatttgctca tcaacgctcg ctcaggtgtc
     2581 ggctttgtgg acgagggaga ggacctgctg cgaaacctcg atatggggca gctcggtcga
     2641 ggtcgactcg gccatcccct ggacgctcca gcgaaccaga tcgcggatgg agacgatgcc
     2701 gatcaggccc tcgtcagaga cgaccggcag gtgccggacg cccgcgcgga ccatctcccg
     2761 ggcggcctcc tgcaccgagg cggccgggct gacggtgatg aggtcgtcgg tgagggcctc
     2821 gtgcgcggcg acgttcttgg gatcctcgcc ggaaccgacc accttgagga tgtcgcgctc
     2881 ggtgaggatg ccgaggaccc ggtcgtcctc cagcacgatc accgagccga tcccctgggc
     2941 gtgcatcatg ctcgccacat cggccacgtt gtactccggg ccgaccgtga cgacctcctt
     3001 ggtcatgatc tcggagacct ccacgaggca ccctccttgc gtgcaggcaa aggttcaggc
     3061 ggcggcccgc aggccgtagc ctggaccccc gagattatcg taggccgtta caggcggcca
     3121 tgacggggca ggcgtcgcta gactgacgcg tcggaagccc tagcgcgctg cccggaacaa
     3181 agacacccaa aaggagagtc tgcgacatgc cctcggattc ctatgacctg gtgatcctgg
     3241 gtgggggcac cggtgggtac tcggccgccc tgcgtggcgc tggcctcggg ctcagcgtcg
     3301 ccctggtcga aaaggacaag gtgggcggca cctgcctgca ccggggatgc atccccacca
     3361 aggccctgct gcacgccgcc gagctggtgg acggcatcca cgaggcggcg gaccgctggg
     3421 gcgtcaagac cgcgatcgag tcgatcgact actccgcgac ggtcgccaac cgcgaggaca
     3481 tcgtcaccaa gaacttcaag ggcctggaag gccacctgaa gaaggaaaaa gtggacatcc
     3541 tcgccggcaa cggcaaggtg accggcccca ggcaggtcga ggtggagggc gtcggtacgg
     3601 tcaccgccgg caaggcgctg gtgctggcct ccgggtcggt gccgaagtcg ctgcctggcc
     3661 tggagatcga cgggcagcgg atcatcacct ccgaccacgc cctgcagctc gactacctgc
     3721 cgaagtcggt gatcgtgctg ggcgcgggcg cggtcggggt cgagttcgcc tccttctacc
     3781 gctcgatggg cgccggcgtg accgtggtcg agatcctgcc cggcatggtt cccctggagg
     3841 acgccgacat ccagaaggag ctgggacggg cgttcaggaa gcgcggcatc gaggtgctgg
     3901 tcgacacccg cttcgaggag gccaaggtcg acggcgacca ggtcaaggcc accgtcacca
     3961 gcaagggcgg caagggcggc aagggcgaca ccaaggagct gaccgccgag ctgctgctgg
     4021 tcgccaccgg ccgcgggccg gtcagcgctg gcctcggcta cgagtcgatc ggcgtcacgc
     4081 tcgaccgcgg ctatgtcgtc cccaagaact gggacaccct ggagaccgac gcggagggcg
     4141 tccacgcggt cggcgatctc ctgcccccgc cctccctcgc gctggcgcac gcctccttcg
     4201 cggagggcat gctggtcgcg gagctggcgg ccgggctgcc gtcgccgccg atcgactacg
     4261 cgggcgtggc gcgggtgacc ttctcgaccc cggaggtcgc cagcgtcggg ctgaccgagg
     4321 cgcaggcgcg cgagcagggc tacgaggtgg tcaccaaccg gttcccgttc aacggggtcg
     4381 ccaagggcat gatccacggc cagggcggca tggtgaagct ggtcgcggcc aaggacggcc
     4441 cggtgctcgg cgtccatctg gtcggcgcgc gcgtcaccga gctcgtctcc gaggccatgc
     4501 tggtctacaa ctgggaggcg ctgccgagcg acgtcgccca gttcatccac ccgcatccga
     4561 ccctgtcgga ggcggtcggg gaagcgtacc tgaccctggc cggccggccg ctgcaccagg
     4621 gctgaccttc gagtctcaaa ggaagcgagg aggccatggc cacgccagtg aagctgccgg
     4681 agctgggcga gtccatcacc gaggggacca tcacccgctg gctgaaacag gaaggcgacc
     4741 gcgtcgaggc tgacgagccg ctctacgagg tctccaccga caaggtcgac accgaggtcc
     4801 ccgccccggt cgccggggtg ctgcgcgcca tcaaggtgca ggaggacgag accgtcgcgg
     4861 tcgggaccga gctggccgtg atcgagcagg gcggcgaggc cgacggcgac ggcgccggcg
     4921 ccggcgccgg cacggcgcag gccgcggccg agccggaagc cgagaccgag ccggaggcgg
     4981 aaacggaagc gcaggctgaa ccggaggcgg agccggcccc acagccgaag gctgaggctg
     5041 aaccggaacc ggaggcggag gccgcggaga agcctcagcg tgaacctcaa ccgcaggctc
     5101 aggctgagcg tgacggcggg cgggacggcc ggagtggcgc tggcgcgcag gcctcgccgc
     5161 tggtccgccg gctcgcgcgc gagcagggcg tggatctgag ccgggtggag ggcaccggcc
     5221 cggggggccg ggtgaagcgc gaggacatcg acgcctacct cggccagcgc ggcgagcagc
     5281 cggctgcggc caaggccaag gctccggcaa gggcagcgga ggcacccgcc cctggcgccg
     5341 gccccgcgcc ggcgggcccg gcgccccggg tggccgtgcc gctggcgcag gggctgcgcg
     5401 aggaggtggt gcgctcgacc cggctgcggc gcctgatcgc cgagcggatg gtcagctcgc
     5461 tgcagaccag cgcccagctc accgccgccg tcgaggtcga catgacccgg gtgatgcgcc
     5521 tgcgggaacg tgccaaggac cagttcaagc agcgcgaggg cctgtcgctc tcgccgctgc
     5581 cgttcgccgt caaagcgttg atcgccgcgg tcaagcagta cccgagcgtg aactccgctc
     5641 tggacgagga cggcaacctg catatctacc aggacgtcca cctcggcgtc gcggtcgaca
     5701 ctcccaaagg cctgttcgtg ccggtgatca agggagccga cagcctcaac ctggtcggcc
     5761 tgacccgggc gatcgcggac ctggcgcggc gcacccgcga cggcagcatc ggcccggacg
     5821 agctgtcggg atccaccatc acggtgacca ataccggcag ccgcgggacc gtctgggaca
     5881 ccccaatcat caaccagccg aacgcggcca tcttcgccac cccggcgatc gtcaagcggc
     5941 cctgggtgat cgacgacccg gacctcggcg agatcatcgt ggtgcgccac atcatggagg
     6001 cgatcctcac ctacgaccac cgcatcatcg acggcgccga cgcggcccgc ttcctgggcg
     6061 cgatcaaaca ggtgctggag ggcgacgact tcaccaacga gctggccgcc gagctcggcg
     6121 tgaaggacgc gggcaagaac aagggctgag gaggcggggc tgatggagcc gctgtggacg
     6181 gtccgggccg ggctggtgcc ctatcaccgt gcctgggcgt ggcagcgggc gctggtggag
     6241 cggcgcgccg ccggggagat ccccgacacg gtgctgctgc tggagcaccc gcacgtcttc
     6301 accatcggca agcgcggcgg cgacgccgac gtgctggtga gcgaggcgtg gctggccgcc
     6361 cagggcgccg aggtggtcca cagcgaccgg ggcggccagg tcacctacca cggccccggc
     6421 cagctggtcg gctaccccat caccctgctg gagcccggac cgggcgtgtg gccgttcatc
     6481 cgccgtgtcg aggacgccct ctgcgacgtc gccgccgagt tcggcctggc ggcccggggc
     6541 gagggcggcc tgctcaccgg cgtctgggtc ggcgagggtg ccgccgaggc gaagctcgct
     6601 gcgatcggga tgcgggtgtc gcgcggggtg accagccacg gcttcgcgct caactgcgac
     6661 accgatctgt ccaagttcaa cgcgatcgtc ccgtgcggga tgcccgacaa acccgcctgc
     6721 tccctgtcga gcctactcag ccgccaggtc accgtcgccg aggtgctgcc gctggccgaa
     6781 cgccacctcg ccgaacgcct ggggcgggac gctgtccccg tggacctcgc tacgctggag
     6841 cttccagagt ccgatcccgc cgctgtccca taggtgcagg tgcgagagac aaatggctga
     6901 acccgtcatc cccgccggag tcaagaccct gtcggtgctg ccccggaccc cggcgccgcc
     6961 gccgaccgag cagacctt
//
LOCUS       DASWDC010000332         8098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_472804, whole genome shotgun sequence.
ACCESSION   DASWDC010000332 DASWDC010000000
VERSION     DASWDC010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472804"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2595
                     /locus_tag="VF995_10655"
     CDS             <1..2595
                     /locus_tag="VF995_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEX9344061.1"
                     /translation="GAVLEGHAGEWEELARLTRTDLDALADAIDEADATEVRGLDGLD
                     PATLRGQARDLADHLASGGRTGGLLKAPAVRAARPLLDHVRVDGLVPDTPELLARLIR
                     VLDAEVGLARIERRWGTRLPGAVRAAGGTANGRANGHARPQPRPDGQPSGQRHATAPA
                     RSAAPATAYSIRRARLVDAADALQGVLALAGPQAAAEAAAQAVPGLIPPLWSDAARVQ
                     ELVRCLQALDAEREVAAITEAIQAAAGAYRAASLLRGTAPECAYAASALHARDLAAYS
                     SAFAGIEEVADGKALTERRDALLARLRAGAPQLAAALERQPDDPAWDERLAELERAWD
                     WARAEGWYRGMVNPAEERAIGERLRACDQRSLKLTGELGANLAWRHCLARMSHEQSQH
                     LRAYQTAMRRYGKGTGKHAPTHLAAAQRHMEACQDAVPAWIMPTYRVAETIPPRPHAF
                     DVVIVDEASQSGVDALFLLWLAPKVVVVGDDRQISPDNIGVDRYVVERLQRQHLPDVE
                     LRDLLGLDNSLYDQTASRYQGRIWLQEHFRCMPEIIEFSNQLSYGDHLLVPLRQFGTD
                     RLEPLRTVHVANAITYGNDLGKINDDEAVAIVDRIARCCADPRYDGRSMGVVSLLGAA
                     QAKRVHDLLVERIGPEEMVARRLKCGTAYDFQGDERSVMFLSMVVVANPDGRPLPALG
                     GRGSEQRFNVAASRAQDQLWLFHRVQPHDLNRECVRWKLLSHCLNPPPAENLRQVAGE
                     IDADALQEPFETLFEQQVCRRLTARGYRVVAQHRIHGFRVDLVVLGERSRLGLVCDGD
                     QWRGPDQYDHDLARQRALERCGWRFHHLRASEFSRDPDGALEPLWQLLADRGISPADP
                     PQAATVRL"
     gene            complement(2590..2859)
                     /locus_tag="VF995_10660"
     CDS             complement(2590..2859)
                     /locus_tag="VF995_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9344062.1"
                     /translation="MIYLDTSAFVKLVQVEQETPALESFLSQRSGAPLVSSALLVVEA
                     RRAALRASPSQARLVDSLLTRVSKVEISDALIEAASRLPDPMPGS"
     gene            complement(2856..3140)
                     /locus_tag="VF995_10665"
     CDS             complement(2856..3140)
                     /locus_tag="VF995_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9344063.1"
                     /translation="MVSIGIRELRQHASRYVRMAKEGRRIAITDRGTLVAYLVPPDEQ
                     PAGLLERMEARGELTPAIGSRLDLFPPPPLAEGERPLSEALQELRDEERW"
     gene            complement(3164..5164)
                     /locus_tag="VF995_10670"
     CDS             complement(3164..5164)
                     /locus_tag="VF995_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9344064.1"
                     /translation="MAVRYGHGGVGGGGRGRGMLGSRMPSGPEADRSLREVLSDRQVL
                     RRAARLFRPYRLQVAIVTVAILVTAGLGVANPLLIRATFDKALFVPGGPRIRLLVTLV
                     SLMIAIPVVSSLVGVYQSWLTNTTGQSVMRDLRATLYAHLQSMPLRFFTATRTGEIQS
                     RLANDVGGLQVVITDVASSVLSNLVILLSTLVAMLVLSWQLTVLSLFLLPLFVVLTRR
                     VGRARRRVTGETQEALAEMSATTEETLSVSGVLLAKVFGRQKSEAERYGEQNERLARL
                     QVRQQMIGRSFFAVVSTFFSITPALVYLVAGLTYRHGGRLSPGTLVAFTTLQARLFMP
                     IGQVLQISTEVASSLALFSRIFDYLDMHPDIVDAPGAVALPADRVRGEVALDQVWFSY
                     DAAPSSLSAVSDGPPPRSRWALRDLDLRVEPGMLAAIVGPSGAGKTTISYLIPRLYDT
                     TRGRVLLDGHDVRQLTLASLSDAIGMVTQESYLFHASVRRNLLYARPHATDEELAAAA
                     TAAYIHDRIMELENGYDTVVGERGYRLSGGEKQRLAIARVVLKDPRVLILDEATSSLD
                     TASERIVQAALQPLMRGRTTIAIAHRLSTILAADVIFVLDRGRLVEQGSHAELLAHGG
                     LYRTLYDEQFGGGAVEARCHNGLVMSNGQVISIPETGTEALT"
     gene            5418..6797
                     /locus_tag="VF995_10675"
     CDS             5418..6797
                     /locus_tag="VF995_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9344065.1"
                     /translation="MPKDRSEVSRREFLRTTGTLAAAGAGASVLAACTGPAGSSSAKG
                     GKQLRILQWSHFVPSYDTWFDGWAKQWGKDNGITVTVDHIDNSLLPARTAAEINAGQG
                     HDLIEWIVPPSSLEPSVIDVSDIVQQTIGKYGDQVSFAKLSSFNPTTNKYYSFGHTWT
                     PDPGDYRKSLWSKVGLPNGPSTYDELLSGGTELKRSQGIPVGIGMSQEVDSNMAARGL
                     MWSFGGSIQDAQQNVVINSPEVIDAVDYMAKLFKQSMTDEIFSWTAASNNQGLIAGKL
                     NYILNSISAYRSAQGSNPAVANDIFFRTALRGPKTALVAQHVVRSYIVPKFSKQVDTA
                     KKFLLALADQSHNVTYNSKLYDFPSFKNAPAWKELPGWLDNDPFSSQPANKLALLKDA
                     ESWSVNVGHPGPANAAESEIFTTFVIPNMMADAAHGMPAKDAVAKAEAAIKPIYAKWR
                     QQGLVGGTS"
     gene            6835..7893
                     /locus_tag="VF995_10680"
     CDS             6835..7893
                     /locus_tag="VF995_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9344066.1"
                     /translation="MAVVETREVRKVFTDTPAVDGIDLRTEEGEYLVLLGPSGSGKTT
                     LLRMIAGLERPTAGEVRIGGQVVNDLPPRARQIAMVFQSYALYPHKTVFANIAFPLAA
                     RGVAKEDRPERVRWAAQLLGIGHLLDRKPRQLSGGERQRVALARALVREPQVFLLDEP
                     LSNLDAKLRASAREDLKEFQRRVGITTIYVTHDQVEAMGLGDRIAVLDHGRLQQVGTP
                     WEIYHEPANIFVATFVGSPPMNLIEQEGVLFGFRPEHFLPAGLVAAGGGKVLLDYKVD
                     RVEYLGSDRLVYGTIPALSPGTRVISRLPFTVTDPVEDGTTAHFALAASSVRCFSKES
                     GERVEAKPLLGSSGGTVS"
     gene            7890..>8098
                     /locus_tag="VF995_10685"
     CDS             7890..>8098
                     /locus_tag="VF995_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344067.1"
                     /translation="MTAAAEAGTRVRRQRYLLDREGVLGPLLLLPAVAYLAILVAFPL
                     VMAIVYAFTSITTGNPALHFVGLQT"
BASE COUNT         1273 a         2912 c         2835 g         1078 t
ORIGIN      
        1 ggcgcggtcc tggagggcca cgccggcgag tgggaggagc tggcgcggct cacccggacc
       61 gacctcgacg ccctcgccga cgcgatcgac gaggccgacg ccaccgaggt ccgcggcctc
      121 gacgggctcg acccggcgac cctccgcggc caggcccgcg acctggccga ccatctcgcg
      181 tccggaggcc ggacgggcgg cctgctcaag gcgccggcgg tgcgcgccgc ccggccgctg
      241 ctggaccacg tccgcgtcga cgggctcgtg cccgacaccc cggagctgct ggcccgcctc
      301 atccgggtgc tcgacgccga ggtcggcctc gcccggatcg agcgccgctg gggaacgcga
      361 cttcccggtg ccgtgcgggc ggcgggcggc accgccaacg gcagggccaa cgggcacgcc
      421 cgcccgcaac cgcgacccga tgggcagccc agcgggcagc gccacgccac cgccccggcc
      481 cggagcgccg cgccggccac ggcctactcg atccgccggg cccggctggt ggacgccgct
      541 gacgcgctcc aaggcgtgct ggcgctcgcc gggccacagg cggcggcgga ggcggccgcg
      601 caggcggtcc ccgggctgat cccgccgctg tggtcggacg ccgccagggt gcaggagctg
      661 gtccgctgcc tccaggcgct ggacgccgaa cgcgaggtgg cggcgatcac cgaggcgatc
      721 caggccgccg ccggcgccta ccgcgccgcc agcctgctgc gcggcaccgc gccggagtgc
      781 gcctatgccg ccagcgcgct gcacgcccgc gacctggccg cctactccag cgccttcgcc
      841 ggcatcgagg aggtcgccga cggcaaagcg ctcaccgagc ggcgcgacgc gctgctggca
      901 cgcctgcgcg ccggtgcccc gcagctggcc gccgcgctgg agcgccagcc tgacgacccg
      961 gcctgggacg agcgcctggc cgagctggag cgggcctggg actgggcccg cgcggaaggc
     1021 tggtaccggg gcatggtcaa ccccgccgag gagcgcgcca tcggcgagcg gctgcgcgcc
     1081 tgcgaccagc gctccctcaa gctcaccggg gagctgggcg ccaacctcgc ctggcggcac
     1141 tgcctggcac ggatgagcca cgagcagtcg cagcacctgc gcgcctacca gaccgccatg
     1201 cgccgctacg gcaagggcac gggcaaacac gcccccaccc atctcgccgc cgcccagcgg
     1261 cacatggagg cctgccagga cgccgtcccc gcctggatca tgccgaccta ccgggtcgcc
     1321 gagacgatcc cgccgcgccc gcacgccttc gacgtggtga tcgtggacga ggccagccag
     1381 tccggggtgg acgcgctgtt cttgctgtgg ctcgcgccga aggtcgtggt ggtgggcgac
     1441 gaccggcaga tcagcccgga caacatcggc gtcgaccgct acgtggtcga gcggctgcaa
     1501 cgccagcacc tgcccgacgt ggagctccgc gacctgctcg gcctggacaa cagcctctac
     1561 gaccagaccg ccagccgcta ccagggccgc atctggctgc aggagcactt ccgctgcatg
     1621 ccggagatca tcgagttctc caaccagctc agctacggcg accacctgct ggtgccgctg
     1681 cgccagttcg gcaccgaccg gctggagccg ctgcgcaccg tccacgtggc caacgccatc
     1741 acctacggca acgatctcgg gaagatcaac gacgacgagg cggtcgcgat cgtcgaccgg
     1801 atcgcgcgct gctgcgccga ccctcgctac gacggccgct ccatgggcgt ggtcagcctg
     1861 ctcggcgccg ctcaggccaa gcgggtccac gacctgctgg tcgagcggat cggcccggag
     1921 gagatggtcg ccaggcggct caagtgcggc accgcctacg acttccaggg cgacgagcgc
     1981 tcggtgatgt tcctgtcgat ggtggtggtc gccaaccccg acgggcggcc gctgcccgcc
     2041 ctcggcgggc ggggttcaga gcagcgcttc aacgtcgccg ccagccgcgc ccaggaccag
     2101 ctgtggctgt tccacagggt ccagccccac gacctcaacc gcgagtgcgt gcgctggaag
     2161 ctgctgagcc actgcctgaa cccgccgccc gccgaaaacc tgcgccaggt cgccggcgag
     2221 atcgacgccg acgccctgca ggagcccttc gagaccctct tcgagcagca ggtctgccgg
     2281 cgcctcaccg cccgcggcta ccgggtggtg gcccagcacc ggatccacgg tttccgggtc
     2341 gacctggtgg tgctcggcga gcgctcccgc ctggggctgg tctgcgacgg cgaccagtgg
     2401 cgcggccccg accagtacga ccacgacctc gcccgccagc gcgccctgga acgctgcggc
     2461 tggcgcttcc accatcttcg cgccagcgag ttctcacgcg accccgacgg cgccctcgaa
     2521 cccctctggc agctcctcgc cgaccggggc atcagcccgg ccgacccgcc gcaagcggcg
     2581 accgtgcggc tatgagccgg gcatcgggtc gggcagacgg ctggcggcct cgatcagagc
     2641 atcggagatc tccaccttgc tgacccgagt gagcagcgaa tcgacgaggc gtgcctgact
     2701 ggggctcgct cgcagggcag cccggcgtgc ctccacgacc aagagcgcgc tggagaccaa
     2761 cggtgcgccc gaccgctggg agaggaagga ctccagcgca ggcgtctcct gctcaacctg
     2821 gacgagcttc acgaacgccg aggtgtcaag gtaaatcacc agcgctcctc gtcgcgaagc
     2881 tcttgcaggg cttcgctgag cggacgctcc ccctctgcca gtggaggcgg tggaaacagg
     2941 tcaaggcggc taccaatcgc gggagtcaat tcaccccgcg cctccatccg ctccaggagt
     3001 ccggctggct gctcgtcagg gggcacgaga taggcgacaa gcgtgccacg gtccgtgatc
     3061 gcaatacgac gcccctcctt cgccatacgg acgtagcggg aggcgtgctg gcgcagctca
     3121 cggatgccga tcgagaccat gtggacattg tagctcctat gtgctacgtc agagcctccg
     3181 ttccggtctc cgggatgctg atcacctggc cgttggacat gaccaggccg ttgtggcagc
     3241 gggcctcgac ggcgccgccg ccgaactgct cgtcgtagag cgtgcggtag aggccgccgt
     3301 gggcgagcag ttcggcgtgg gagccctgct cgaccagccg gccgcggtcg agcacgaaga
     3361 tcacgtcggc ggcgaggatg gtggagaggc ggtgggcgat cgcgatcgtc gtgcggcccc
     3421 gcatcagcgg ctgcagggcc gcctgcacga tccgttcgct ggcggtgtcc agggatgacg
     3481 tcgcctcgtc caggatcagc acccgggggt ccttgaggac cacccgggcg atggcgagac
     3541 gctgcttctc cccgccggag aggcggtagc cgcgctcccc caccacggtg tcgtagccgt
     3601 tttccaactc catgatgcgg tcgtggatgt aggcggcggt ggcggcggcg gccagctcct
     3661 cgtcggtcgc gtgcggccgg gcgtagagga ggttgcgccg cacgctggcg tggaacaggt
     3721 agctctcctg ggtgaccatg ccgatcgcgt cgctgagact ggcgagggtc agctgccgca
     3781 cgtcgtggcc gtccagcagc acccggcccc gggtggtgtc gtagagccgg gggatcaggt
     3841 aggagatggt ggtcttgccg gccccgctcg gccccacgat cgcggccagc atcccgggct
     3901 cgacccgcag gtccaggtca cgcagcgccc agcgggaacg cggcgggggg ccgtcgctga
     3961 ccgctgacag cgacgacggc gcggcgtcgt aggagaacca gacctggtcg agggcgacct
     4021 cgccgcggac ccggtcggcg ggcagggcga ccgcgccggg ggcgtccacg atgtcggggt
     4081 gcatgtcgag gtagtcgaag atgcgcgaga agagcgccag cgagctggcc acctcggtgg
     4141 agatctgcag cacctgcccg atcggcatga acaggcgcgc ctgcagggtg gtgaaggcga
     4201 ccagggtccc ggggctgagg cgcccgccgt ggcggtaggt cagcccggcg accaggtaga
     4261 ccagcgcggg ggtgatcgag aagaaggtcg agaccaccgc gaagaacgac cggccgatca
     4321 tctgctggcg cacttggagg cgggcgaggc gctcgttctg ctcgccgtag cgctcggctt
     4381 cgctcttctg gcggccgaac accttggcga gcagcactcc ggagaccgac agggtctcct
     4441 cggtggtggc gctcatctcg gcgagcgcct cctgggtctc gccggtgacc cggcggcgcg
     4501 cccggccgac ccggcgggtc aggacgacga acagcggcag caggaacagc gaaagcacgg
     4561 tgagctgcca gctcagcacc agcatcgcca ccagggtgga gagcaggatc accaggttgg
     4621 agagcacgct ggaggcgacg tcggtgatca ccacctggag gccgccgacg tcgttggcga
     4681 ggcgggactg gatctcgccg gtgcgggtcg cggtgaagaa gcgcagcggc atgctctgca
     4741 ggtgggcgta gagggtggcg cgcaggtcgc gcatcacgct ctggccggtg gtgttggtca
     4801 gccagctctg gtagacgccg acgaggctgg agacgaccgg gatggcgatc atcaggctga
     4861 ccagcgtcac cagcaggcgg atcctggggc cgccggggac gaacagcgcc ttgtcgaagg
     4921 tcgcgcggat cagcagcggg ttggccacgc cgagcccggc ggtgaccagg atcgccacgg
     4981 tgacgatcgc gacctggagg cggtagggcc ggaacaggcg cgccgcccgg cgcagcacct
     5041 gccggtcgct gaggacctcc cgcaggctgc ggtcagcctc cgggccggag ggcatcctgg
     5101 acccgagcat tccccggcct ctgccgccgc ccccgacgcc gccatgcccg tagcgcaccg
     5161 ccatggccgc tccttccccg cgcgccctcc acattgcctt ccccagtgtc gtccgggatg
     5221 cgccagaccg cacggaaggc gcattcgcag gaaggagctt ggcccgcaat ccagcaaaat
     5281 atatgatgca tcccgacagc gacgctgtat ctggcgtcca gagcaagcgg gacgcttcaa
     5341 ccgtgcgcat tcgggtatga gccagtcggg tatgagccag tcgtgtcggt gaccgtgatg
     5401 gtcacgagga ggcgaccatg ccgaaagacc gcagcgaagt ctctcgtcgc gaattcctga
     5461 ggaccaccgg cacactcgcc gccgcgggcg ccggcgcgag cgtcctggcg gcgtgcaccg
     5521 ggcccgcggg atcctccagc gcgaagggcg ggaagcagct ccggatcctg cagtggagcc
     5581 atttcgtccc cagctacgac acctggttcg acggctgggc caagcagtgg ggcaaggaca
     5641 acggcatcac cgtgaccgtc gaccacatcg acaactccct gctccccgcc cgcaccgccg
     5701 ccgagatcaa cgccggccag ggccacgacc tgatcgagtg gatcgtgccg ccgtcgtcgc
     5761 tggagccgag cgtgatcgac gtcagcgaca tcgtccagca gaccatcggc aagtacggcg
     5821 accaggtcag cttcgccaag ctctccagct tcaacccgac caccaacaag tactacagct
     5881 tcgggcacac ctggacgccc gacccgggcg actaccgcaa gagcctctgg agcaaggtcg
     5941 gcctgccgaa cgggccctcc acctatgacg agctgctcag cggcggcacc gagctcaaga
     6001 ggagccaggg catcccggtc ggcatcggca tgtcgcagga ggtcgactcc aacatggcgg
     6061 cccggggcct gatgtggtcg ttcggcgggt cgatccagga cgcgcagcag aacgtggtga
     6121 tcaactcccc cgaggtgatc gacgcggtcg actacatggc caagctgttc aagcagagca
     6181 tgaccgacga gatcttcagc tggaccgccg cctccaacaa ccaggggctg atcgcgggca
     6241 agctcaacta catcctcaac tcgatctccg cataccgcag cgcgcagggc tccaacccgg
     6301 cggtggccaa cgacatcttc ttccggacgg cgctgcgggg ccccaagacg gcgctggtcg
     6361 cccagcacgt ggtgcgcagc tacatcgtcc ccaagttctc caagcaggtg gacaccgcca
     6421 agaagttcct gctggcgctg gccgaccagt cgcacaacgt cacctacaac tccaagctgt
     6481 acgacttccc ctcgttcaag aacgcccccg cctggaagga gctgcccggc tggctcgaca
     6541 acgacccctt cagctcgcag ccagccaaca agctcgcgct gctcaaggac gcggagagct
     6601 ggagcgtcaa cgtcggccac ccgggccccg ccaacgcggc cgagagcgag atcttcacca
     6661 ccttcgtgat cccgaacatg atggccgacg ccgcccacgg catgcccgcc aaggacgccg
     6721 tggccaaggc ggaggcggcc atcaagccga tctacgcgaa gtggcgccag cagggcctgg
     6781 tcggcggcac ctcctgatga ccaggcgcaa cctcccgggg gggcagggct cgccatggcg
     6841 gtagtggaaa ccagggaggt ccgcaaggtc ttcaccgaca cccctgcggt cgacgggatc
     6901 gacctgcgca ccgaggaggg cgagtacctg gtgctgctgg gcccgtcggg gtccggcaag
     6961 acgacgctgc tgcggatgat cgccgggctg gagcgcccca ccgccgggga ggtgcggatc
     7021 ggcgggcagg tggtcaacga cctgcccccg agggcgcggc agatcgccat ggtgttccag
     7081 agctacgccc tctacccgca caagacggtg ttcgccaaca tcgccttccc gctcgccgcc
     7141 cggggggtgg cgaaggagga ccggcccgag cgggtccgct gggccgccca gctgctcggc
     7201 atcggccacc tgctcgaccg caaaccgcgc cagctctccg gtggggagcg ccagcgggtc
     7261 gcgctcgcgc gggcgctggt gcgcgagccg caggtgttcc tgctggacga gccgctctcc
     7321 aacctggacg ccaagctgcg cgcctccgcc cgcgaggacc tcaaggagtt ccagcggcgg
     7381 gtcggcatca ccacgatcta cgtcacccac gaccaggtgg aggcgatggg cctcggcgac
     7441 cgcatcgccg tgctcgacca cggccgcctg cagcaggtcg gcaccccctg ggagatctac
     7501 cacgagccag ccaacatctt cgtcgccacc ttcgtcggct ccccgccgat gaacctgatc
     7561 gagcaggagg gcgtcctgtt cggcttccgg cccgagcact tcctgcccgc cgggctggtg
     7621 gcggccggag gcgggaaggt gctgctcgac tacaaggtcg accgggtcga gtacctcggc
     7681 tctgaccggc tcgtctacgg caccatcccg gcgctctcgc cgggcacccg ggtgatctcc
     7741 cggctgccgt tcacggtcac cgacccggtg gaggacggca ccaccgccca cttcgcgctc
     7801 gctgcctcaa gcgtccgctg cttcagcaag gagagcgggg agcgggtcga ggccaagccg
     7861 ttgctgggga gttcgggggg cacggtctcg tgacggccgc ggcggaggca ggcacccggg
     7921 tacgacggca gcgctacctg ctcgaccggg agggcgtcct cgggccgctg ctgctgttgc
     7981 cggcggtggc ctacctggcg atcctggtcg cgttcccgct ggtgatggcg atcgtctacg
     8041 cgttcaccag catcaccacc gggaacccgg cgctgcactt cgtcgggctg cagacctt
//
LOCUS       DASWDC010000333         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_149117, whole genome shotgun sequence.
ACCESSION   DASWDC010000333 DASWDC010000000
VERSION     DASWDC010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149117"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /gene="carB"
                     /locus_tag="VF995_10690"
     CDS             complement(<1..778)
                     /gene="carB"
                     /locus_tag="VF995_10690"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390636.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEX9344068.1"
                     /translation="MPLRDDLRSILVIGSGPIVIGQAAEFDYSGTQACRALRRLGLRV
                     ILVNSNPATIMTDPEVADATYLEPLTPGSVAAVLEAERPDALLPTLGGQTALNLAVAL
                     HERGDLERFGVELIGATVEAVRRAESRVAFSEVCRAAGLDLPASVEVGTLEQGREAAA
                     ELGLPLVLRPSFTLGGWGGGIAYDAAALDELLAQGLAASPVRRVLVEESVLGWKEFEL
                     EVMRDRADNAVVVCSIENLDPMGVHTGDSITVAPAMTLSDR"
     gene            complement(778..1158)
                     /locus_tag="VF995_10695"
     CDS             complement(778..1158)
                     /locus_tag="VF995_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344069.1"
                     /translation="MTEQRTFRTLGGVDAGTLPPAGEAAEALRGEFDRALKLKTSLRG
                     EADAAVSPTGRHATAGDEAKLRILVASCEGASRYAIKLGLLTPGQVRALWSEAIAKGL
                     YDGWDGGQSAYNPPNRNPDPEGAS"
     gene            complement(1155..>1877)
                     /locus_tag="VF995_10700"
     CDS             complement(1155..>1877)
                     /locus_tag="VF995_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEX9344070.1"
                     /translation="ALSTETLDPEKLQAAALAAPDPNGLNLIAEVTRATPLELPPDPG
                     PGERHRVVLYDYGAKANIIRRLRRLGARVLVVPATTPAAETLAWEPDGVMLANGPGDP
                     ATLREEVAAVGELLGRVPIFGICLGHQILGQAVGGRTVRLKFGHRGINQPVVEVATGR
                     VAVTSHNHGFAVDADSLPRGVQVTHRNANDGVVEGLECGDLPAFSVQYHPEAAPGPHD
                     AHPLFERFAALMDQRRGGGSRR"
BASE COUNT          227 a          634 c          761 g          255 t
ORIGIN      
        1 cgcggtccga cagggtcatg gcgggcgcga cggtaatcga gtcgccggtg tggacgccca
       61 tcgggtcgag gttctcgatg gagcagacca ccaccgcgtt gtcggcgcgg tcgcgcatca
      121 cctccagctc gaactccttc cagccgagca ccgactcctc caccagcacc cgccggactg
      181 ggctggcggc caggccctgg gcgagcagct cgtccaacgc cgcggcgtcg taggcgatcc
      241 cgccgcccca gccgccgagg gtgaaggagg gccgcagcac cagcggcagg ccgagttcgg
      301 cggcggcctc gcggccctgc tccagggtcc cgacctcgac gcttgccggg aggtcgagcc
      361 cggcggcgcg gcagacctcg ctgaaggcga cgcgggactc ggcgcggcgg accgcctcga
      421 cggtggcgcc gatcagctcc accccgaagc gttccaggtc gccgcgctca tgcagggcga
      481 cggcgaggtt gagggcggtc tgcccgccga gggtcggcag cagcgcgtcg ggccgctcgg
      541 cctccagcac cgccgcgacc gagccggggg tgagcggttc caggtaggtg gcgtcggcga
      601 cctccgggtc ggtcatgatc gtggcggggt tggagttgac caggatcacc cgcaggccga
      661 gccgccgcag cgcccggcag gcctgcgtgc cggagtagtc gaactcggcg gcctggccga
      721 tcacgatcgg cccggagccg atcaccagga tcgagcggag gtcgtcgcgc agcggcacta
      781 ggaggcgccc tccgggtccg ggttccggtt cggtgggttg taggcggact ggccgccgtc
      841 ccagccgtcg tagaggccct tggcgatcgc ctcggaccac agcgcgcgga cctgcccggg
      901 ggtgagcagg ccgagcttga tggcgtagcg gctggcgccc tcgcagctgg caaccaggat
      961 gcggagcttg gcctcgtccc cggcggtggc gtggcggccg gtgggcgaca cggcggcgtc
     1021 ggcctcgccc cgcaggctcg tcttgagctt gagggcgcgg tcgaactcgc cgcgcagcgc
     1081 ctcggccgcc tccccggccg ggggcagggt gccggcgtcg acgccgccga gggtgcggaa
     1141 ggttcgttgc tcggtcatcg gcgggaacct cctccgcgac gctggtccat gagcgccgcg
     1201 aatcgctcga acaggggatg ggcgtcgtgg gggccgggag cggcctccgg gtggtactgg
     1261 accgagaagg cgggcaggtc gccgcattcc aggccttcga ccacgccgtc gttggcgttg
     1321 cggtgggtga cctggacgcc tcttgggagg ctgtcggcgt cgaccgcgaa gccgtggttg
     1381 tggctggtga ccgcgacccg gcccgtggcg acctccacga ccggctggtt gatgcctcgg
     1441 tggccgaact tgagccgcac cgtgcggccg ccgaccgcct ggccgaggat ctggtggccg
     1501 aggcagatcc cgaagatcgg cacccggccg agcagctcgc cgacggcggc gacctcctcg
     1561 cggagggtgg cggggtcgcc ggggccgttg gcgagcatca ccccgtcggg ttcccaggcg
     1621 agcgtctcgg cggcgggggt cgtggccggg acgaccagga cgcgggcgcc gaggcggcgc
     1681 agcctgcgga tgatgttggc cttggcgccg tagtcgtaga gcacgacccg gtggcgctcg
     1741 cccgggcccg ggtcgggcgg cagctccagg ggggtcgcgc gggtgacctc ggcgatcagg
     1801 ttgaggccgt tggggtcggg ggcggcgagg gcggcggcct ggagtttctc ggggtcgagg
     1861 gtctcggtgg agagggc
//
LOCUS       DASWDC010000334         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_986858, whole genome shotgun sequence.
ACCESSION   DASWDC010000334 DASWDC010000000
VERSION     DASWDC010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986858"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="VF995_10705"
     CDS             complement(<1..1467)
                     /locus_tag="VF995_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9344071.1"
                     /translation="MLRATLKGLLAHRVRLILTACAIALGVGFMAGTFILTATISHGI
                     DNMFASASAGTDVIVRPVASSTGSSGGNSNSQSLARSTVPASLVSQVRQVRQVAGVAA
                     ADGATSDRAGIVGSDGKVIGGRSGFAVSYPPDAALASAYPLRQGRAPAGPTEVAIDAA
                     TAAQQGLHVGDRVRVVAGGRARPFTISGIVGFGQADGPGVVGMVAFDAVTAQQLYGRH
                     NTYDEIDLKAAAGVTPAALRSRVAGVLPSDDEAVTAASAAATQASDLRGQISFLTNGL
                     LAFALISLFVGAFIIWNTFSILVAQRTRELALLRALGASRRQVLRSVLVEAAVLGLVA
                     SLAGLGLGVLAASGLQALLRGASINLPSTGLDLPVGDAALAVGTGVLITLVAAFAPAR
                     KATLIVPIAALRTTTPATGGSSTRRLVSGAAVAVAGGAALLAGLFGHTSAGSLLVGGG
                     ALAAFIGVTVLCPLFAKPLAGAIGAPLRRLPGRTGVLAR"
     gene            complement(1474..2211)
                     /locus_tag="VF995_10710"
     CDS             complement(1474..2211)
                     /locus_tag="VF995_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007818908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9344072.1"
                     /translation="MSTAVALAARAVEVTKRFGSGDNAVTALDHVSIEIARATFTAVM
                     GPSGSGKSTLMHCLAGLDAVSGGQIWIGGTEITSLPERRLTRLRRDQVGFVFQAYNLL
                     PALSAAANITLPLDIAGRRPDNSWYDTVIAAVGLQQRLSHRPAELSGGQQQRVAVARA
                     LVTKPEIVFADEPTGNLDSRASAEVLDLLRRSVREFGQTVVMVTHDPVAASYADHVVF
                     LADGRVVGDLPAPTPERVLDFMKQLGG"
     gene            complement(2427..3101)
                     /locus_tag="VF995_10715"
     CDS             complement(2427..3101)
                     /locus_tag="VF995_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9344073.1"
                     /translation="MTTVKVLLADDQPLLRTGFRMILEAEPDIEVVGEAGDGETAIRL
                     ARRLAPDVVLMDIRMPVMDGVQATRELANTDHLKVLILTTFNLDEYVVEALRAGASGF
                     LLKDVPAAELVNAIRLVAAGDAVVAPAVTRRLLDRFAHRLPGAEEQRQAVPETLASLT
                     EREAAVLRLVARGLSNTEIAAELVVSDATVKTHVHHLLSKLGLRDRVQLVVFAYEAGL
                     IRPQTG"
BASE COUNT          422 a         1028 c         1331 g          407 t
ORIGIN      
        1 gcgggcgagc accccggtgc ggccgggcag gcgccgcaac ggcgcgccga tcgccccggc
       61 gagcggcttg gcgaacagcg ggcagagcac ggtcacgccg atgaaggcgg cgagcgcgcc
      121 gccgccgacc agcaggctcc cggcgctggt gtggccgaac aggccggcga gcagcgcggc
      181 gcccccggcc accgccacgg cggcgccgct gacaaggcga cgggtggaag acccccccgt
      241 ggccggggtg gtggtgcgca gcgcggcgat cgggacgatc agggtggcct tgcgggccgg
      301 cgcgaaggcg gcgaccaggg tgatgaggac gccggtgccg accgcgagcg cggcgtcgcc
      361 gaccggcagg tcgaggccgg tgctgggcag gttgatgctg gcgccgcgca gcagcgcctg
      421 caggccgctg gccgccagca ccccgaggcc gaggccggcc agggaggcga ccaggccgag
      481 cacggcggcc tccaccagga ccgagcggag cacctggcgg cgactcgcgc cgagcgcgcg
      541 cagcagcgcc agctcgcggg tgcgctgcgc caccaggatc gagaaggtgt tccagatgat
      601 gaaggcgccg acgaacaggg agatcagcgc gaaggcgagc aggccgttgg tgaggaagct
      661 gatctggccg cggaggtcgg aggcctgggt ggcggcggcc gaggcggcgg tgacggcctc
      721 gtcgtcgctg ggcagcacgc ccgcgacccg gctgcgcagg gcggcggggg tgacgccggc
      781 ggcggccttg aggtcgatct cgtcgtaggt gttgtggcgg ccgtagagct gctgggcggt
      841 gacggcgtcg aaggcgacca tgccgacgac gccggggccg tcggcctggc cgaagccgac
      901 gatgccgctg atggtgaagg gccgggcgcg gccgccggcc accacgcgga cgcggtcgcc
      961 gacgtggagg ccttgctggg cggcggtggc cgcgtcgatg gcgacctcgg tggggccagc
     1021 gggggccctg ccctgccgca gcggataggc ggaggcgagg gccgcgtccg gcgggtagct
     1081 gaccgcgaag ccggaacggc cgccgatgac cttgccgtcg ctgccgacga tcccggcgcg
     1141 gtcggaggtg gcgccgtcgg cggcggcgac gccagcgacc tggcggacct ggcggacctg
     1201 gctgacgagg ctggcgggga cggtcgagcg ggcgagggac tggctgttgc tgttgccgcc
     1261 gctgctgccg gtgctggagg cgaccgggcg gacgatcacg tcggtgccgg cgctggcgga
     1321 ggcgaacatg ttgtcgatgc cgtggctgat ggtggcggtg aggatgaagg tgccagccat
     1381 gaagccgacg ccgagcgcga tcgcgcaggc ggtgaggatc aggcggacgc gatgggcgag
     1441 caggcctttg agggtggcgc ggagcatcgg ggatcagcct cccagctgct tcatgaagtc
     1501 gaggacgcgc tcgggggtgg gagcgggcag gtcgccgacg acgcggccgt cggcgaggaa
     1561 gacgacgtgg tcggcatagc tggcggcgac cgggtcgtgg gtgaccatga ccacggtctg
     1621 gccgaactcg cgcaccgagc ggcgcagcag gtcgaggacc tcggcgctgg cgcgggagtc
     1681 gaggttgccg gtgggctcgt cggcgaagac gatctcgggc ttggtgacca gcgcgcgggc
     1741 gaccgcgacg cgctgctgct ggccgccgga aagctcggcc ggccggtggc tgagccgctg
     1801 ctggaggccg accgcggcga tcacggtgtc gtaccagctg ttgtcggggc ggcggccggc
     1861 gatgtcgagc ggcagggtga tgttggcggc cgcgctcagc gccggcagca ggttgtaggc
     1921 ctggaagacg aagccgacct ggtcgcggcg gagccgggtc aggcgccgct cggggaggct
     1981 ggtgatctcg gtgccgccga tccagatctg gccgccgctg acggcgtcga ggccggcgag
     2041 gcagtgcatc agcgtcgact tgcccgagcc cgacgggccc atcaccgcgg tgaaggtggc
     2101 gcgggcgatc tcgatggaga cgtggtcgag ggcggtgacc gcgttgtcgc cgctgccgaa
     2161 gcgcttggtc acctcgacgg cgcgggcggc aagcgcgacg gcggtgctca tggtggtggg
     2221 ttcggcggtt gcgccgatgg cgccggggcg cgccgtcgtc tcggtcatcg ctggctcctc
     2281 tcggaggcgt gctgtgcctt ctctcggaga ggacgctatg gccggcggcg gctggcgtcg
     2341 tcggccgcgc gatctgggtt tcgcctgcat ccttgggtcg aggcagaggc gtcctccacc
     2401 ttcaggtgga gaccggcggc gaggcgctag cctgtctggg gacggatcag gccggcctcg
     2461 taggcgaaga cgaccagctg gacgcggtcg cgcaggccga gcttgctgag caggtggtgg
     2521 acgtgggtct tcacggtcgc gtcggagacg accagctcgg cggcgatctc ggtgttggac
     2581 aggccgcggg cgaccagcct gagcacggcc gcctcccgtt cggtcagcga cgccagcgtc
     2641 tcggggacgg cctggcgctg ctcctcggcg ccgggcaggc ggtgggcgaa gcggtcgagc
     2701 agccgccggg tcacggcggg tgcgaccacc gcgtcgccgg cggcgaccag ccggatggcg
     2761 ttgaccagct cggccgcggg cacgtccttg agcaggaagc cgctcgcccc ggcgcgcagg
     2821 gcctccacca cgtactcgtc gaggttgaag gtggtgagga tcagcacctt caggtggtcg
     2881 gtgttggcca gctcgcgggt ggcctggacg ccgtccatca ccggcatgcg gatgtccatc
     2941 agcaccacgt ccggtgccag ccgccgcgcc aggcggatgg cggtctcgcc gtcgccggcc
     3001 tcccccacca cctcgatgtc cggctcagcc tccagaatca tccggaaccc ggtccgcagc
     3061 agtggctggt cgtcggcgag cagcaccttg acggttgtca tctgagagcg acctccaacg
     3121 gcaggcgggc acggatctgg tagccgcccc cggggcgcgg cccggcggtc agctcgccgt
     3181 cgaacagg
//
LOCUS       DASWDC010000335         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_405739, whole genome shotgun sequence.
ACCESSION   DASWDC010000335 DASWDC010000000
VERSION     DASWDC010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405739"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1985)
                     /gene="dnaX"
                     /locus_tag="VF995_10720"
     CDS             complement(<1..1985)
                     /gene="dnaX"
                     /locus_tag="VF995_10720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX9344074.1"
                     /translation="MVGQDHIVGTLTNAVQTGQVHHALLFTGTRGTGKTTTARLLAKA
                     LNCERGPTAEPCNQCESCRSITDGASLDVVEIDAASHGSVDDARDLRERANYAPAASR
                     YKVYIVDEAHMVTPQGFNAMLKVLEEPPEHVRFMFCTTEPHKVIEAIRSRCQRHDFRR
                     VRSTVLVDYFGKLCAAEGVDIEPAALELVVKAADGSVRDGLSRLDQVLTFAGKRVSHA
                     DVARLLGVLPVELRFELADALAGHRVGEVLGCVEQVVQAGHDLHHFAREALEHLRDLY
                     VLKVAPESTTLVDTTAETRERLAAQAARFGPGELGRLVRILGDVYQDLRTATDQRLVL
                     EVGLARAAMPEASLDAEALLARIERLERRLAITVNDDGSAPMAPVTPVTPAAPVAPVA
                     PTAPTTPRGGTTAGGTTTAGGPTAPSGPAAIPSVGGPPETSLSSSRAGARGLSTAPSA
                     PSVAPSSIGAASAADADWAVAEPAGPEAPLDFGLVQRSWALVVERVRAGGRVVASYLE
                     HGRLMALEGRQVVLEYAPKDRFYAEALGKDGRSAKVDAALQAVLGSGLTIRLAISETA
                     ASSGSPAPAAPAVTAPARNAPSRPAPPAAAEDQPPPVEFEIDPENDAGEPLDSDADAR
                     AVADWAAQQLGGQVIEERPNPRARGSKGAGKASGPKGS"
     gene            complement(2204..2469)
                     /gene="ffs"
                     /locus_tag="VF995_10725"
     ncRNA           complement(2204..2469)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF995_10725"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            2544..>2856
                     /locus_tag="VF995_10730"
     CDS             2544..>2856
                     /locus_tag="VF995_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9344075.1"
                     /translation="MTPGSAMDAQQESLARIELAFSLLIRLGNRPKMREALAKATGMS
                     LDRAALTVLSSLSEHGASRLSELSPRIGVDTSTACRQVQNLERLGLVDRVADPADRRA
                     SR"
BASE COUNT          411 a         1099 c          932 g          414 t
ORIGIN      
        1 gaccctttcg gtcctgatgc cttccctgct cccttcgacc cgcgggccct ggggttcggt
       61 cgctcctcga tgacctgacc gcccaactgc tgtgcggccc agtctgccac cgcgcgagcg
      121 tccgcgtccg agtcgagcgg ctcgccggca tcgttctccg ggtcgatctc gaactcgacc
      181 ggcggcggct ggtcctcagc ggcggcgggc ggcgccgggc gcgacggtgc gttcctggcc
      241 ggagcggtca cggcgggggc ggcgggggcc ggggatccgc tggaggctgc cgtctcgctg
      301 atcgccagcc ggatcgtcag gccgctgccg agcacggcct gcagggcggc atcgactttc
      361 gcggaacggc cgtccttgcc gagcgcctct gcatagaagc ggtccttcgg ggcgtattcc
      421 agcaccactt ggcggccttc gagcgccatc agccggccat gttcgaggta gctggcgacc
      481 acccgcccgc cggcccgcac ccgctccacc accagcgccc acgagcgctg caccagaccg
      541 aagtcgagcg gcgcctccgg cccggcgggc tcggccaccg cccagtcggc gtcggcggcg
      601 ctcgccgcgc cgattgagga cggagccacc gacggcgccg aaggggctgt ggacagcccg
      661 cgcgcgcccg cccgcgaact tgataggctc gtttcggggg ggcccccgac ggaggggatg
      721 gcggcgggac cactcggcgc ggtcgggcct cctgcggtgg tggtgcctcc tgcggtggtg
      781 ccccccctcg gggtggtcgg tgccgtcggc gccacgggtg ccacaggtgc cgcgggtgtc
      841 acaggtgtca caggtgccat gggcgccgag ccgtcgtcgt tgacggtgat cgccaggcgc
      901 cgttccagcc gctcgatccg tgccagcagc gcctcggcgt ccagtgacgc ctccggcatc
      961 gcggcgcggg caaggcccac ctcgagcacc agccgctgat ccgtcgcggt gcgcaggtcc
     1021 tggtagacgt cgccgaggat acggaccagc cgccccagct cccccggccc gaagcgggcc
     1081 gcctgcgcgg cgagccgctc ccgggtctcc gcggtggtgt cgaccagcgt ggtcgactct
     1141 ggagccacct tcagcacgta caggtcgcga aggtgctcca gcgcctcacg agcgaagtgg
     1201 tgcaggtcgt gcccggcctg gaccacctgc tccacgcatc ccagcacctc gccgacccgg
     1261 tgcccggcga gcgcgtcggc cagctcgaac cgcagctcaa ctggcagcac cccgagcagc
     1321 cgcgccacgt ccgcatggga gacccgcttc cccgcgaagg tcaggacctg atcgagccgg
     1381 ctgaggccgt cacgcaccga cccgtcagcc gccttgacga ccagctccag ggctgccggc
     1441 tcgatgtcga ccccctcggc ggcgcacagc ttcccgaagt agtccaccag caccgtcgac
     1501 cgcacccgcc ggaagtcgtg ccgctggcag cgcgaccgga tcgcctcaat gaccttgtgc
     1561 ggctcggtgg tacagaacat gaaccgcaca tgctccggcg gctcctccag caccttcagc
     1621 atcgcgttga agccctgcgg cgtcaccatg tgcgcctcgt ccacgatgta gaccttgtag
     1681 cgcgaggccg ccggcgcata attggcccgc tcccgcaggt cccgggcgtc atcgaccgac
     1741 ccgtggctcg ccgcgtcgat ctcgaccacg tcgaggctgg cgccgtcggt gatcgaccgg
     1801 caggactcac actggttgca cggctccgct gtcggccccc gctcgcagtt gagcgccttc
     1861 gccagcaacc gcgccgtggt cgtcttccca gtcccccgcg tccccgtgaa cagcaacgca
     1921 tgatggacct ggccggtctg cacagcgttc gtcagcgtcc cgacgatgtg gtcctgcccg
     1981 accacctcgc caaacgtctg cggccgccac ttccgataga gcgcaacgta tcccataagg
     2041 gctctgagtc agcgaagtta ggaagaagtt agcaagaggg agaggcctca gccccaagtc
     2101 gcccgagcat atcaccgccc accgacaccc cctgacccgg cccaccccta ccactggagg
     2161 gagctcgagg gggagccgcg ccgcggctcc ccctcgataa ggaccgtgca cctgccgtcg
     2221 accccggcac ccggagcgct ggccaaagcc ggtggctcgg gcccggcacc cccgcggcac
     2281 acgccagtgc tcacttagcg ctgcttcctc ccggacctga cgcggttcgc gaggtggcgt
     2341 tgcgcgggac ctgaccgtca acactccgta ctcggcgctg acctccggac gcggaccctc
     2401 gggcagggat tcagccccgc tggagcggat tgcgggtaca gggcaccgct agctccccac
     2461 ctagcacggt ccgccccgag cctaccacgc ccgcatcgag gaatttattt gtatgctaca
     2521 accaactact ctaaactggc ggcatgaccc ccggatccgc catggacgcc cagcaggagt
     2581 cgctcgcgcg gatcgagctg gccttttccc tgctgatccg gcttggaaac cgccccaaga
     2641 tgcgggaggc actggccaag gccaccggga tgtcgctcga ccgggcggcg ctcacggtcc
     2701 tctccagcct ttccgagcac ggggcaagcc gcctctccga actttcgccc cgcatcggcg
     2761 tcgacacctc caccgcctgc aggcaggtcc agaacctgga acgcctcggc ctggtcgacc
     2821 gcgtggccga ccccgccgac cggcgcgcca gccgcc
//
LOCUS       DASWDC010000336         3091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1560570, whole genome shotgun sequence.
ACCESSION   DASWDC010000336 DASWDC010000000
VERSION     DASWDC010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560570"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VF995_10735"
     CDS             <1..281
                     /locus_tag="VF995_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9344076.1"
                     /translation="RVADPADRRASRLRLSVEGERVFARVRAARRDAVAAVIAGWTPE
                     QRGGFATLLLELVQGLETAVADGTFGGSPHPAPTEASAAKEATTGVRV"
     gene            278..1918
                     /locus_tag="VF995_10740"
     CDS             278..1918
                     /locus_tag="VF995_10740"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX9344077.1"
                     /translation="MTTTIEQRGDRLSHRQIQIVFLGLMLGMLLAALDQTIVSTALPT
                     IVGDLGGLTHLSWVVTAYLLASTASTPLYGKLGDLYGRKIIFQAAIVIFLAGSALSGL
                     SQNMNELIAFRALQGLGAGGLMASALAIIGDVVAPRERGRYQGYMGGVFAFASVAGPL
                     TGGFFTDHLSWRWVFYINLPIGALALVVTSIVLDLPFRRVQRRIDWLGAGLIVAAVTS
                     ILLVTVWGGDQYAWGSPQILGLAAAGLVLLALFILQERRAEEPILPPHLFANGIFRVS
                     LLLMFLVAIGLFGALVYLPVFLQLATGASATNSGLLLLPLMAGLLCTSIASGQLITRT
                     GHYKWSPLTGTFIMTIGLFLLSRMTIDTTRLTSGLYMLVLGFGLGGLMQVSVLATQNA
                     VDRRDLGTATSAIGFFRNLGGAVGVAGFGAVFTNRLAHYLPQHLPASALGRFNVKALQ
                     GSPSTIHQLPPVLRDGIAQSFAQAIHVVYLVAVPVAALSFVVALFLKELPLRERANIG
                     TNAEAQAPAQAIPVPGTGGDGDEGQGSAPSFEHASAPD"
     gene            complement(1963..2052)
                     /locus_tag="VF995_10745"
     tRNA            complement(1963..2052)
                     /locus_tag="VF995_10745"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2016..2018),aa:Ser,seq:gga)
     gene            complement(2177..2449)
                     /locus_tag="VF995_10750"
     CDS             complement(2177..2449)
                     /locus_tag="VF995_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344078.1"
                     /translation="MVVVDLAEAVAPAVLAAAALLVWRVGRAANAAAALAHHAPVGPA
                     RQAEVAVLLLGTAATLGLAALLVRGQAASPLGRRRVELLDTLVNSC"
     gene            complement(2574..2861)
                     /locus_tag="VF995_10755"
     CDS             complement(2574..2861)
                     /locus_tag="VF995_10755"
                     /inference="COORDINATES: protein motif:HMM:NF025577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4326 domain-containing protein"
                     /protein_id="HEX9344079.1"
                     /translation="MPSRVQLRRTRGWRLPPGAVSVAAPTKWQNPFRPRARSPEANAR
                     AVERYRGHLAEHPELVEAARQELVGKDLACWCAAGLPCHADVLLEIANQGP"
     gene            complement(2907..2996)
                     /locus_tag="VF995_10760"
     tRNA            complement(2907..2996)
                     /locus_tag="VF995_10760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2960..2962),aa:Ser,seq:cga)
BASE COUNT          426 a         1161 c         1036 g          468 t
ORIGIN      
        1 accgcgtggc cgaccccgcc gaccggcgcg ccagccgcct gcgtctcagt gtggagggcg
       61 agcgggtctt cgcgcgtgtc cgggccgccc gccgcgacgc cgtggcggcg gtgatcgcgg
      121 gatggacccc ggagcagcgc ggcggattcg ccaccctgct cctggaactg gtgcagggcc
      181 tcgagacggc ggtggcggac gggaccttcg gaggaagccc tcaccccgcg ccgacggaag
      241 cctcagcagc aaaggaagcc accacaggag tgcgcgtatg acgaccacca tcgagcagcg
      301 tggcgaccgc ctcagtcacc ggcagatcca gatcgtgttt ctcgggctga tgctcggcat
      361 gctgctggca gcgctcgacc agaccatcgt gtcgacggcg ttgcccacga tcgtcggcga
      421 cctcggcggc ctcacgcatc tctcctgggt ggtcaccgcc tacctgctcg cctccaccgc
      481 ctcgacgccg ctctacggca agctcggcga cctctacggc cgcaagatca tcttccaggc
      541 ggcgatcgtc atcttcctgg ccggctcggc gctgtccggg ctgtcgcaga acatgaatga
      601 gctgatcgcc ttccgcgccc tgcaggggct cggcgccggc ggcctcatgg ccagcgccct
      661 ggcgatcatc ggcgacgtgg tggcgccccg cgagcgcggc cgctaccagg gctacatggg
      721 cggcgtgttc gccttcgcca gcgtggccgg gccgctgacc ggcggcttct tcaccgacca
      781 cctctcctgg cgctgggtct tctatatcaa cctgccgatc ggcgcgctgg cgctggtggt
      841 caccagcatc gtcctggacc tcccgttccg gcgggtgcag cgccgcatcg actggctcgg
      901 cgccggcctg atcgtcgcgg cggtcaccag catcctgctg gtcacggtgt ggggcggcga
      961 ccagtacgcc tggggctccc cgcagatcct cggcctcgcc gcggccggcc tggtgctgct
     1021 cgccctgttc atcctccagg agcgccgcgc cgaggagccg atcctgcccc cgcacctgtt
     1081 cgccaacggc atcttccggg tgtcgctgct gctgatgttc ctggtcgcca tcggactgtt
     1141 cggcgcgctc gtctacctgc cggtgttcct gcagctcgcc accggtgcca gcgccaccaa
     1201 ctcgggcctg ctgctgctcc cgctgatggc cgggttgctc tgcacctcga tcgcgtccgg
     1261 ccagctgatc acccgcaccg gccactacaa gtggtcgccg ctgaccggca ccttcatcat
     1321 gaccatcggc ctgttcctgc tctcccgcat gaccatcgac accaccaggc tcacctcggg
     1381 cctgtacatg ctggtcctcg gcttcggcct cggcgggctg atgcaggtgt cggtgctggc
     1441 gacccagaac gcggtcgacc ggcgcgacct cggcacggcc acctcggcga tcggcttctt
     1501 ccgcaacctc ggcggcgccg ttggggtggc gggcttcggc gcggtgttca ccaaccgcct
     1561 ggcccactat ctgccgcagc acctgccggc cagcgccctc ggccgcttca acgtcaaggc
     1621 gctccagggc agccccagca ccatccacca gctcccgccg gtcctgcggg acggcatcgc
     1681 ccagtcgttc gcccaggcga tccacgtggt ctacctggtg gcggtcccgg tcgcggcgct
     1741 gtcgttcgtg gtggccctgt tcctcaagga actccccctg cgcgaacgcg ccaacatcgg
     1801 caccaacgcc gaggcccaag ccccggccca ggcgatcccc gtccccggga caggcggaga
     1861 cggcgatgag ggtcaaggct cggcgccgtc cttcgagcac gcgagcgcgc cggactgagg
     1921 ccggactgag gccgagcccg ctcccagccc cagcgaagct gttggcggag gctgaggggt
     1981 tcgaaccctc gagaaggttg ccctcctaca cgctttccag gcgtgtgcct tcgaccgctc
     2041 ggccaagcct ccgcgcgcag tctagcaacc tgtggtgagg gggcgccgag gtcgtccacc
     2101 cggccttacc ccgcgaagtg ctgggccagg cggacgaccg cgccgtagag gccgacggcg
     2161 cccaggcaca gtgggatcag caggagttga ccagggtgtc gagcagctcg acccgccgcc
     2221 gccccagcgg tgaggccgcc tggccgcgga ccagcagggc ggcgaggccc agggtcgccg
     2281 cggtgccgag cagcagcacc gccacctcgg cctgccgggc gggtccaacc ggagcgtgat
     2341 gggcgagcgc ggcggcggcg ttggcggccc gcccgaccct ccagacaagc agcgcggcgg
     2401 cagcgagcac ggcgggggcg accgcctcgg ccaggtccac gaccaccacc gggccaggcg
     2461 cccgcgaact cggtgtgagg tagacatgtg gtttttaagg aagatcgcga cactaacgca
     2521 gggcactctg ggcgctcacg cctgactcag cccaatcggc cagctccgcc gggttatggc
     2581 ccctggttgg cgatctcgag cagcacgtcg gcatgacagg gcaaccccgc cgcgcaccag
     2641 caggccagat ccttgccgac cagctcctgg cgggcggcct cgaccagctc cggatgctcg
     2701 gcgaggtggc cgcggtaccg ctcgaccgcg cgggcgttgg cctccggcga gcgtgcccga
     2761 ggccgaaacg ggttctgcca cttcgtcggc gcggccaccg acaccgcccc gggcggcagg
     2821 cgccacccac gggtgcggcg aagctggacg cggctcggca cgctcgccct cgacaggctt
     2881 acatcgtctc cacagaatcc acagcgtggc ggcaaggatg ggattcgaac ccatggacgc
     2941 cttgcgacgt caacggtttt cgagaccgcc ccattcgtcc gctctggcac cttgccgtaa
     3001 cggagtatag cggaggtcag gggcgaggcg gctccgcctc cgcctccctc ccatggacgg
     3061 catcgcttcg ctcgcctaca cgctcgggcg g
//
LOCUS       DASWDC010000337         3934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_988889, whole genome shotgun sequence.
ACCESSION   DASWDC010000337 DASWDC010000000
VERSION     DASWDC010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988889"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VF995_10765"
     CDS             complement(<1..573)
                     /locus_tag="VF995_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006143741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9344080.1"
                     /translation="MPLDPAPATARWHCEHAWLGPGRVAAGVLIEVEGERIVRVEAGV
                     PPPPAADREVGPGSGAGAGVRVLRGLTLPGFANAHSHAFHRLLRGRTHRAGGDFWRWR
                     ELMYQAAATLDPDGYRQLARAVFAEMTLAGWTAVGEFHYLHHQPGGRPYQDPNAMGEA
                     LCAAAADAGIRLTLLDTCYLWSGFGRRVPMQ"
     gene            complement(564..1802)
                     /locus_tag="VF995_10770"
     CDS             complement(564..1802)
                     /locus_tag="VF995_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007460873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEX9344081.1"
                     /translation="MSETEEKAQFEESFGSLAAIGRNEVSGGWTRFAWTPEDAEARVW
                     FIRTARAAGAKVEADRDGNLWALAGPAGAADRLVATGSHLDTVRNGGAFDGALGVVSG
                     LLALRRIVASGIALKRGIAVIAFVDEEGGRFGTPTLGSELLCGEREPADVLDRADPRG
                     VTMREAMDAAGVNTGNLGGDEARLARLDAYVELHVEQGRGLADLGSPLAVGTGVWPHG
                     RWRFDAVGEANHAGTTRLADRRDPMLVLADAVAEARRLAGGLQAVATVGRVEVAPNVT
                     NAVPERAAFWVDVRAPDEATLERLVAELGRAVRLSAARNGVQVEVARESRSAGTSFDG
                     PLLRRLGDAVEAVGATPCRLPTAAGHDAAVLARHLPAAMLFVRNPTGTSHSPAEHAST
                     EDCVVGVRALAAALQDLACR"
     gene            1883..2773
                     /locus_tag="VF995_10775"
     CDS             1883..2773
                     /locus_tag="VF995_10775"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9344082.1"
                     /translation="MTVTGSDIEVELLVDAHAVVGEGPAWDAALRRLVWVDILRGQVH
                     LYDPATGTNDQADVGKAVGAAVPRASGGLVLALEDGFAAFDPATEQLTPLAPVEADQP
                     GLRMNDGKCDRAGRFWAGSMEWDGEHPAGSLYRLDPDHRVTRMVEGVRISNGLGWSPD
                     ERTMYYIDSPTHRVDVFDFDVESGSLAGRRTLVELPLEWGLPDGMTVDADGFLWVAYW
                     GGWSVRRLAPDGTLATTLRLPVSQVSSCAFGGPDGTDLYITSASNELTAEQLRAEPHA
                     GALFRARPGVAGGPMHAFGG"
     gene            2888..>3934
                     /locus_tag="VF995_10780"
     CDS             2888..>3934
                     /locus_tag="VF995_10780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9344083.1"
                     /translation="MAATSTYKGLSFWHDSLPEPLGRRPALPGDLDVDVAVVGAGYSG
                     LWTAYYLAKADPGLRVAVLEREIAGFGASGRNGGWCSALFPASLEKVARVAAGGDRGG
                     GDRGGGDPRAAAVALERAMQRTVDEVGRVAAAEGIDCHWAKGGTVSLARTPVQLDRAK
                     EHVAHARAWGFGDDDLRLLGPDEAGAMVNATRVLGGTWTPHCAAIHPARLARGLAAAV
                     ERLGVKLYEGTPVLEIQPGAVRTASGTVKAERIVRATEGYSPRLPGLRRAVAPVYSLM
                     IATEPLPASTWERIGLASRPTFTDYRHLIIYGQRTADGRFAFGGRGAPYHFGSKIRPE
                     FDREPAVFAELRRVL"
BASE COUNT          492 a         1472 c         1490 g          480 t
ORIGIN      
        1 ctgcatgggc acccggcgtc cgaaccccga ccacaggtag caggtgtcca gcagggtgag
       61 gcggatgccg gcgtcggccg ccgccgcgca cagcgcctcg cccatcgcgt tcgggtcttg
      121 gtagggccgc ccgccgggct ggtggtgcag gtagtggaac tcccccaccg cggtccagcc
      181 ggccagcgtc atctcggcga acaccgcgcg ggcgagctgc cggtagccgt ccgggtccag
      241 ggtggccgcc gcctggtaca tcagctcccg ccagcgccag aagtcgccgc cggcccggtg
      301 ggtgcggccc cgcagcagcc ggtggaaggc atgggagtgc gcgttggcga accccggcag
      361 ggtgaggccc cgcagcaccc gcacaccggc ccccgcgccg ctgcctggac ccacctcccg
      421 gtccgcggct ggaggcgggg gcacgccagc ctccacccgg acgatgcgct cgccctccac
      481 ctcgatcaag accccagctg ccacccgccc cgggccgagc caggcatgct cgcagtgcca
      541 gcgggccgtc gccggcgccg gatctagcgg cacgcgaggt cctggagggc ggccgccagg
      601 gcgcggacgc ccacaacgca gtcctcggtg ctggcgtgct cggctgggct gtggctggtg
      661 ccggtggggt tgcggacgaa cagcatggcg gcggggaggt gcctggcgag cacggcggcg
      721 tcgtggccgg cggcggtcgg caggcggcag ggcgtggcgc cgaccgcctc gaccgcgtcg
      781 ccgaggcggc gcagcaacgg cccgtcgaag ctggtgccgg cgctgcggga ctcgcgggcg
      841 acctcgacct ggacgccgtt gcgcgccgcc gacagccgca ccgcgcggcc cagctcggcg
      901 accagccgct ccagggtggc ctcgtcggga gcgcggacgt cgacccagaa ggcggcccgc
      961 tccgggaccg cgttggtgac gttgggggcg acctcgaccc ggccgacggt ggcgaccgcc
     1021 tgcagcccgc cggccaggcg gcgggcctcg gcgaccgcgt cggcgagcac cagcatcggg
     1081 tcgcggcggt cggccaggcg ggtggtgccg gcgtggttgg cctccccgac cgcgtcgaag
     1141 cgccagcgcc cgtgcggcca caccccggtc ccgaccgcca gcgggctgcc gaggtcggcg
     1201 aggccgcggc cctgctcgac gtgcagctcc acgtaggcgt ccaggcgtgc caggcgcgcc
     1261 tcgtcgccgc cgaggttgcc ggtgttcacc ccggcggcgt ccatcgcctc gcgcatggtg
     1321 acgccgcgcg ggtcggcgcg gtcgagcacg tcggcgggtt cgcgctcgcc gcagagcagc
     1381 tcgctgccga gcgtcggggt gccgaagcgg ccgccctcct cgtcgacgaa ggcgatcacg
     1441 gcgatcccgc gcttgagggc gatcccgctc gccacgatcc gccgcagcgc cagcagcccg
     1501 gagaccaccc cgagcgcccc gtcgaaggcg ccgccgttgc gcaccgtgtc cagatggctc
     1561 ccggtcgcga ccagccggtc agcggcaccg gcgggcccgg cgagcgccca caggttgccg
     1621 tcgcggtcgg cctccacctt ggcacccgcc gcccgcgcgg tgcggatgaa ccagacgcgc
     1681 gcctcggcgt cctcaggggt ccaggcgaac cgggtccagc cgccggacac ctcgttgcgg
     1741 ccgatcgccg ccagggaccc gaaggactcc tcgaactggg ccttctcctc ggtctcgctc
     1801 atcggagcct agagtatcgg gcatccgccg ccgccgaggg ccgccggcat cacaacttct
     1861 gtggagaagg gagcgccgag aggtgaccgt gaccgggagc gacatcgagg tcgagctgct
     1921 ggtggacgcc cacgccgtcg tcggggaggg gcccgcctgg gacgcggcgc tgcggcggct
     1981 ggtctgggtg gacatcctcc gggggcaggt acacctgtac gacccggcca ccgggaccaa
     2041 cgaccaggcc gatgtcggca aggcggtggg cgcggcggtg ccgcgcgcct cgggcgggct
     2101 ggtgctggcg ctggaggacg gcttcgccgc cttcgacccg gcgaccgagc agctgacgcc
     2161 gctcgcgccg gtcgaggcgg accagccggg gctgcgcatg aacgacggca agtgcgaccg
     2221 cgccgggcgc ttctgggcgg gcagcatgga gtgggacggc gagcacccgg ccgggtcgct
     2281 gtaccggctc gatcccgacc accgggtgac caggatggtc gagggggtgc ggatctccaa
     2341 cgggctcggc tggagccccg acgagcggac catgtactac atcgacagcc ccacccaccg
     2401 cgtcgacgtg ttcgacttcg acgtcgagag cggcagcctc gccggccgcc gcaccctcgt
     2461 cgagctgccc ttggagtggg gcctgccgga cgggatgacg gtggacgccg acgggttcct
     2521 gtgggtggcg tactggggcg gctggtcggt gcggcgcctc gcccccgacg ggaccctggc
     2581 cacgaccctg cggctgccgg tcagccaggt gtcgagctgc gccttcggcg ggccggacgg
     2641 gaccgacctg tacatcacct cggccagcaa cgagctgacc gcggagcagc tccgcgccga
     2701 gccgcacgcc ggcgcgctgt tccgcgcccg gccgggagtg gccggcgggc cgatgcacgc
     2761 cttcgggggc taggtccggg cctagtcata tcgggctgat tctctccttc ggttgatgtc
     2821 aggcagatga cgcggatcaa gccaaccgga ggcgacggcc cgccaactct gtggatacga
     2881 tgagccgatg gctgcgacca gcacatataa aggactgtcg ttctggcacg acagcctgcc
     2941 cgagccgctc gggcggcggc cggcgttacc cggcgacctc gacgtggacg tcgcggtggt
     3001 cggggcgggc tatagcgggc tatggaccgc ctactacctc gccaaggccg acccgggcct
     3061 gcgcgtcgcg gtgctggaac gcgagatcgc cgggttcggc gcctccggcc gcaacggcgg
     3121 ctggtgctcg gcgctgttcc ccgccagcct ggagaaggtc gcccgggtcg ccgccggcgg
     3181 cgaccgcggc ggcggcgacc gcggcggtgg cgacccgcgc gcggcggcgg tcgcgctgga
     3241 gcgggcgatg cagcggaccg tcgacgaggt cggccgggtc gccgccgccg aggggatcga
     3301 ctgccactgg gccaagggcg gcaccgtgtc gctggcccgc accccggtcc agctggaccg
     3361 tgccaaggag cacgtcgccc acgcccgcgc ctgggggttc ggcgacgacg acctgcggct
     3421 gctcggtccc gacgaggcgg gcgccatggt caacgcgacc cgggtgctgg gcggcacctg
     3481 gacgccccac tgcgccgcca tccacccggc ccggctggcc cgcgggctgg ccgccgccgt
     3541 cgaacggctc ggcgtcaagc tctacgaagg caccccggtg ctggagatcc agccgggcgc
     3601 ggtccggacc gccagcggca ccgtcaaggc ggagcggatc gtccgcgcca ccgagggcta
     3661 cagcccccgc cttccgggcc tgcggcgcgc ggtcgcgccg gtctactcgc tgatgatcgc
     3721 caccgagccg ctgcccgcct cgacttggga gcggatcggg ctggcgagcc ggccgacctt
     3781 caccgactac cgtcacctga tcatctacgg gcagcgcacc gccgacggcc gcttcgcctt
     3841 cgggggccgc ggcgccccct accatttcgg ctcgaagatc cgtcccgagt tcgaccgcga
     3901 acccgccgtg ttcgccgagc tgcgccgcgt cctc
//
LOCUS       DASWDC010000338         6970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_990160, whole genome shotgun sequence.
ACCESSION   DASWDC010000338 DASWDC010000000
VERSION     DASWDC010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990160"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VF995_10785"
     CDS             complement(<1..394)
                     /locus_tag="VF995_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016934150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9344084.1"
                     /translation="MPARVRLDRELVRRGLAPSREAASQAVLDGKVLVAGSLAAKPSA
                     LVSPADPVELAGPARRWASRGGDKLAPALERLAVPVAGRRCLDAGASTGGFTDVLLAA
                     GAASVIAVDVGYGQLDLRLRGDPRVVVLD"
     gene            complement(396..539)
                     /locus_tag="VF995_10790"
     CDS             complement(396..539)
                     /locus_tag="VF995_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344085.1"
                     /translation="MRTTMTEQVEAALAALDQHEADLAEHPAIFEAVNDAILAELDQL
                     EGL"
     gene            complement(570..1031)
                     /locus_tag="VF995_10795"
     CDS             complement(570..1031)
                     /locus_tag="VF995_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX9344086.1"
                     /translation="MASAREQYLQALASLGEMTRTQAERVTNLLAKQGELQSGQVGRA
                     TEELMRRVAKNRETVSRLVQREVKRQIGALGIATRDEVARLQQRVRALEQAVERASAS
                     SATSATGASRPARASRPRTAASTASRSKAKPKPKASGASEAGGDDQVRDQG"
     gene            1206..1550
                     /locus_tag="VF995_10800"
     CDS             1206..1550
                     /locus_tag="VF995_10800"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX9344087.1"
                     /translation="MASKSEVGDALERLMARLDGNETNVRSAIPGHKVLRCRVPDVGS
                     TWYSVIDDGHVSPPIEQPPDDGHVDVTLQVNSDDLIDLVEERTSFLSAFLSGKVRVDA
                     SFTDLLRMRNLL"
     gene            1570..2379
                     /locus_tag="VF995_10805"
     CDS             1570..2379
                     /locus_tag="VF995_10805"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9344088.1"
                     /translation="MVSLIPIHDENPTSRVAVITYLLIAINFVVFITEPVATSLNGTP
                     TTAAAACHQIAYFREYSAIPKELTSNQQLPVTAGPPVGPNQCSVVRARYHKIPALSVL
                     YSMFLHGSWLHLLGNMLFLWVFGNNIEDRLGRVRYLLFYLLCGYIAAYGFALGDPNST
                     TTLIGASGAIAGVLGAYLILFPRARVTSLVPFLLFLPIRFPAWVVLGSWFLLQYFYFR
                     GAGVGEGAGVAYLAHVVGFIAGIILVILFGGLSARRRPPPSWNRYQRYPYA"
     gene            complement(2370..3728)
                     /locus_tag="VF995_10810"
     CDS             complement(2370..3728)
                     /locus_tag="VF995_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX9344089.1"
                     /translation="MGAKVEGGPPATATAPGGPLAGRYRSVVFDLDGVVYLGDQAIPD
                     APETLEGVRGLGVRVGFVTNNALRPASAVADKLNRLGVKASAEEVLTSADAVVRLLGG
                     PEGLAGRRVLALGGPGLLEALEGAGARLLDPRDWRAAELVVAGLDPALTYDKVRAATL
                     AIAAGARFVGSNADASLPTPEGPWPGAGSVLALLETATGVRPAVAGKPEPALFEAAAA
                     RLGAAPILMVGDRDDTDLAGAKALGWDVALVLSGVTRPERLLDLPAAPDHLLADVGGL
                     LAPPGPLIRSAGGAEAEAAMALLATEGHGGHGGHGGHGGHGVPDVPAVLLVAVDQQDG
                     RVVGAVACRLPGGEGEDGAAGRGEALLDGVVVAAERRGALIGTRLLLAASLTLRARGP
                     GRGRGAQAIVARRTSDTSAAGFLTRLGFHPATDGALVRELRPAARPQAGSEAAVEGEP
                     QA"
     gene            complement(3742..4263)
                     /locus_tag="VF995_10815"
     CDS             complement(3742..4263)
                     /locus_tag="VF995_10815"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9344090.1"
                     /translation="MGAGRQRSCGRCSKGGGPAARKRRIEEGLIEADAGASDAYELFR
                     RGSELLAAHHAHQAVIVLERARDLQPDKGSVCEALGRAYYATGRLSSAGAQFRRTLEL
                     DPSNDYAHFGLALCLARTGERRHALGHLRLALVMRPGIRAYQRALGPLERAVSAEGAP
                     GRGPDSDAGMAGG"
     gene            4262..5539
                     /locus_tag="VF995_10820"
     CDS             4262..5539
                     /locus_tag="VF995_10820"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX9344091.1"
                     /translation="MVAVAPFRGLRFDPAVTGDPGLVVTPPLDVITPQLRTAYEQRSR
                     HNVIRLSPGSGGRGDVPDDPGQYDQVAKLLAAWREEGALRLDPRPSLYLYEETYEVGG
                     EQRAQRGVLSSVQLDDSRRWVLPHEGTLAPLVADRLRLLEATRANLSPVFGLYAGSGR
                     TAALLDRMAAAAPAVHATDPAGTGHRVWEITDPAIISAWSGLLAAERVLVADGHHRWR
                     AALTYRDAMRRIDSGRRDAPWERTLAYLVDADHAGPAVLANHRLLKGVPGGSVLAALA
                     GDFDAVPLASAAEIEGGLAELPREALAFGLYTPRESWLLVARDPVALAAETGLGRWPL
                     DVEVLHGPVLAGRLGVRDTGRQVVSSGADLDRAAQRVDANGGCSSLLALRPAPFRDVM
                     AVAQAGETLPAKTTFFHPKPRDGLVLRPLEPEE"
     gene            5543..6502
                     /locus_tag="VF995_10825"
     CDS             5543..6502
                     /locus_tag="VF995_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9344092.1"
                     /translation="MSVLDALPDGVIGIDTHMGGISEITAGYLLPAERPALIETGPAK
                     VAGEIAAGVTRAGLDAADLAWIVVSHIHLDHAGGVGDLLRTFPNATVVVHPAGARHLV
                     DPERLLASSARVYGALMDTVYGGLTPVEADRIKAAEDGETLDLGGRRLELLHAPGHAK
                     HHMAVFEPDLGALFTGDGVGVLLPSSPTGVLRPATPPPDFDRDLAVASLRRMAKLDPA
                     HVVLTHFGPIAPPAERLAEAEERLLRWCETAEAAAREHGPEVDHIEAALRERFEQEEG
                     IAADAPEGEQGRLLNSFHSNAAGLARWIEVRDRDRNRNRDQRR"
     gene            complement(6547..>6970)
                     /locus_tag="VF995_10830"
     CDS             complement(6547..>6970)
                     /locus_tag="VF995_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344093.1"
                     /translation="QAEEYKAARTELAAAQRISGGVELSAMLADIERATGRPEQAIEL
                     FRSTDRTRMEADTAAELLIVAASAYGDLDQPAAGVALIRRHARWPAQLAAHHLRLAYA
                     EGDLAERAGDRAGAREAFARVVRADPEFFDAAARLERL"
BASE COUNT          935 a         2781 c         2310 g          944 t
ORIGIN      
        1 ggtccagcac caccacccgc gggtcgccgc gcaggcgcag gtcgagctgg ccatagccga
       61 cgtcgaccgc gatcaccgag gcggcgcctg cagccagcag cacgtcggtg aacccgccgg
      121 tggaagcgcc ggcgtccagg cagcggcgcc ccgccaccgg gaccgccagc cgctccagcg
      181 ccggggcgag cttgtcgccg ccgcggctgg cccagcgccg cgctggtccg gccagctcga
      241 ccgggtcggc cggtgagacc agcgccgacg gcttggccgc gagcgagccg gcgaccagga
      301 ccttgccgtc aagcacggcc tggctggccg cctcccgcga gggggccagc ccgcggcgga
      361 ccagctcccg gtccagccgc accctggcgg gcacgctaga gcccttcaag ctggtcgagt
      421 tcggccagga tggcgtcgtt gacggcctcg aagatggccg gatgctcggc gaggtcggcc
      481 tcgtgctggt cgagggcggc gagcgcggcc tcaacctgct cggtcatggt cgtgcgcatt
      541 gggccagtat aggcactggc accttggcgc tagccctggt cccgcacctg atcgtcgccg
      601 ccagcctcgg atgcgccact ggccttgggt tttggcttgg ccttggatcg agaggcggtc
      661 gaggcggccg tgcgaggacg gctggcccgc gcgggccggc tggcgccggt ggcactggtg
      721 gcgctgctgg cggaggcgcg ctccaccgcc tgctccaagg cgcggacccg ctgctgcagg
      781 cgcgccacct cgtccctggt cgcgatgccg agcgcaccga tctgccgttt cacctcacgc
      841 tggaccaagc ggctgaccgt ctcacggttc ttggccaccc gccgcatcag ctcctcagtg
      901 gcgcggccga cctgaccgga ttgcaactcg ccctgcttgg ccagcaggtt ggtgacccgt
      961 tcggcctgcg tccgggtcat ctcgccgagg cttgccagtg cttggaggta ctgctcccgt
     1021 gcgctcgcca actccacacc tccacgggcc ggagattcgt gcgccgattc tccagccggg
     1081 gggaccaccg ggcaagtgcc gagaacggtc cggtcacaag gcaagatagt gtggtataca
     1141 tcgccggcca ccgccacacc gcgtggcgac aggcgccgat gtcgagacgc aagcgaggag
     1201 gccgcgtggc gagcaagtcc gaggtgggcg acgcgctgga gcggctgatg gcacgcctgg
     1261 acggcaacga gacgaacgtc cgctcggcca tccccggcca caaggtgctg cgctgccgcg
     1321 tccccgacgt gggcagcacc tggtactcgg tgatcgacga cgggcacgtc tcgccgccga
     1381 tcgagcagcc ccccgatgac gggcacgtcg acgtcacgct ccaggtcaac tccgacgacc
     1441 tgatcgacct ggtggaggaa cggacctcgt ttctctcggc gttcctgtcc ggcaaggtcc
     1501 gggtcgacgc gagcttcacg gacctgctgc gcatgcgcaa tcttttgtga ccaaccggaa
     1561 gccggtgcga tggtcagcct gatcccgatc cacgacgaga acccgaccag ccgggtggcg
     1621 gtgatcacct acctgctgat cgccatcaac ttcgtcgtct tcatcaccga gccggtcgcc
     1681 accagcctca acggcacccc caccaccgcc gccgcggcat gccaccagat cgcctatttc
     1741 cgcgagtact cggccatccc caaggagctg acctccaacc agcagctccc agtcaccgcc
     1801 gggccgccgg tcgggcccaa ccagtgctcg gtggtccggg cccgctacca caagatccca
     1861 gcgctgtcgg tcctctactc catgttcctg catgggagct ggctgcacct gcttggcaac
     1921 atgctgttct tgtgggtgtt cggcaacaac atcgaagacc gcctcgggcg ggtccgctac
     1981 ctgctcttct acctgttgtg cggctatatc gcggcctacg ggttcgccct tggggacccc
     2041 aacagcacca ccacgctgat cggtgcctcg ggcgcgatcg cgggggtcct cggcgcctac
     2101 ctgatcctgt tcccgcgcgc ccgggtcacc agcctggttc ccttcctgct gttcctgccg
     2161 atccgattcc ccgcgtgggt ggtgctcggc tcctggttcc tgctgcagta cttctacttc
     2221 cgcggcgccg gggtcggcga gggcgcgggg gtcgcctacc tcgcgcatgt ggtcgggttc
     2281 atcgccggca tcatcctggt gatcctgttc ggcgggctca gcgctcgccg ccgcccgccg
     2341 ccgagctgga accgctacca gcgctacccc tacgcctgag gctcaccctc aaccgcggct
     2401 tcagatcctg cttgagggcg agcggcggga cgtagctccc ggaccagcgc gccatccgtc
     2461 gccgggtgga acccgagccg ggtgaggaat cccgccgcgc tggtgtcgga ggtgcggcgc
     2521 gccacgatgg cctgggctcc ccgtccccgt cccggtcccc gggcgcgcaa cgtcaggctg
     2581 gccgccagca gcagccgcgt gccgatcagc gcgccacggc gctcagccgc caccacgacg
     2641 ccgtcgagca gcgcctcccc cctgccagcg gcgccgtcct cgccctcgcc gccgggcaac
     2701 cggcatgcga ccgctccgac gacccggccg tcctgctggt ccaccgcgac cagcaggacg
     2761 gcggggacgt cggggacccc gtggcctccg tggcctccgt ggcctccgtg gcccccatgg
     2821 ccctccgtgg cgagcagggc catggcggct tcggcctccg cgccgccagc ggagcggatc
     2881 agcggaccgg gtggcgccag caggccgccc acgtcggcga gcaggtggtc gggtgccgca
     2941 ggcaggtcga gcaggcgctc cggccgggtc accccgctga gcaccagcgc gacatcccag
     3001 ccgagcgcct tggcgccggc caggtcggtg tcgtcgcgat cgcccaccat caggatcggc
     3061 gcggcgccga gccgtgcggc cgccgcctcg aacagggcag gctcgggctt gccggccacc
     3121 gccgggcgga ccccggtggc ggtctccagc agcgccagta ccgaaccggc gcccggccag
     3181 ggcccctcgg gggtgggcag gctggcgtcg gcgttgctgc cgacgaaccg ggcccccgcc
     3241 gcgatcgcca gcgtcgccgc ccgcaccttg tcgtaggtca gcgccgggtc gagcccggcc
     3301 accaccagct cggccgcccg ccagtcgcgc gggtcgagca gccgcgcacc agcgccctcc
     3361 agcgcctcca gcagccccgg gccgccaagt gccagcaccc gccggccggc cagcccctcc
     3421 ggcccgccaa gcagccgcac caccgcgtcc gccgaggtca gcacctcctc ggcactcgcc
     3481 ttgaccccga gccggttgag cttgtccgcc accgccgagg ccggccgcag cgcgttgttg
     3541 gtcacgaacc cgacccgcac cccaagccca cgcacgccct ccagcgtctc cggagcatcc
     3601 gggatcgcct ggtcgccgag gtagacgact ccgtccaggt cgaacacgac cgagcggtag
     3661 cgcccggcca gcgggccgcc cggcgctgtg gctgtggccg gcggcccgcc ctccaccttc
     3721 gcgcccacct ccgcgcccac ctcagccgcc ggccatgccg gcgtcgctgt ccggcccgcg
     3781 tccgggggcg ccttcggcac tgaccgcacg ttccagcgga ccgagggcgc gctggtaggc
     3841 gcggatgccg ggccgcatga ccagcgcgag ccgtagatga ccgagggcat ggcgacgctc
     3901 gccggtccgg gcgaggcaca gcgccagccc gaaatgggcg tagtcgttgc tcgggtccag
     3961 ctccagcgtg cggcggaact gcgccccggc cgacgacagc cgaccagtcg cgtagtatgc
     4021 ccggccaagc gcctcgcaca ccgagccctt gtcgggctgc aggtcccgcg cccgctccag
     4081 cacgatcact gcctgatggg cgtgatgcgc tgccagcagc tcgctcccgc ggcgaaacag
     4141 ctcgtaggcg tcggacgcgc cggcgtcggc ctcgataaga ccctcctcga ttcgacgttt
     4201 cctggcggcg ggaccaccgc ccttgctaca acggccgcat gaccgctgac gccctgcccc
     4261 catggtagcc gtcgccccct ttcgcgggct tcgctttgat ccagccgtga ccggcgaccc
     4321 cggcctggtg gtcacccctc ccctcgacgt gatcaccccc cagctccgca ccgcctacga
     4381 gcagcgcagc cgccataacg tcatccggct ctcccctggt agcggcgggc gcggcgacgt
     4441 ccccgacgat cccggacaat acgaccaggt cgccaagctg ctcgccgcgt ggcgagagga
     4501 gggcgccctg cgcctcgacc cgcggccctc cctctacctc tacgaagaga cctacgaggt
     4561 gggtggcgag cagcgcgcgc agcgaggcgt tctcagctcg gtccagctgg acgactctcg
     4621 gcgctgggtc ctgccgcacg agggcaccct ggcgccgctg gtcgccgacc ggctgcgcct
     4681 gctggaggcg acccgtgcca acctctcccc ggtgttcggg ctctacgccg gcagcggccg
     4741 gaccgccgcg ctcctcgacc ggatggccgc tgccgcgcca gcggtccatg ccaccgaccc
     4801 ggccgggacc gggcaccggg tctgggagat caccgacccg gccatcatca gcgcctggag
     4861 tggcctgctc gccgccgagc gggtgctggt cgccgacggc caccaccgct ggcgcgccgc
     4921 gctcacctac cgcgacgcga tgcgccgcat cgattcgggg cggcgcgacg cgccctggga
     4981 gcgcaccctc gcgtacctgg ttgacgccga ccacgccggg ccagcggtcc tcgccaacca
     5041 tcggctgctc aagggtgtcc ctggcggaag cgtcctggcc gcgctcgccg gcgacttcga
     5101 cgcggttccg ctcgccagcg ccgccgagat cgagggcggc ctcgcggagc tgccccgtga
     5161 ggcgctcgct ttcggcctct acacccccag ggagagctgg ctgctggtcg cccgcgaccc
     5221 ggtcgcgctc gccgccgaga ccggccttgg gcgctggccg ctcgacgtcg aggtcctcca
     5281 cgggccagta ctcgctggtc ggctcggcgt ccgcgacacc ggccgccaag tcgtctcctc
     5341 cggcgccgac ctcgatcgcg ccgcccagcg ggtcgacgcc aacggcggct gcagcagcct
     5401 gctggccctg cggcccgccc cgttccgcga cgtcatggcg gtcgcccagg ctggcgagac
     5461 cctcccagcg aagaccacct tcttccaccc caaaccacgc gacgggctgg tgctgcggcc
     5521 cctggaaccg gaggagtagc tcatgagcgt cctcgacgcc ctgcctgacg gggtcatcgg
     5581 catcgacacc catatgggtg gcatctcgga gatcaccgcg ggatacctgc tccccgccga
     5641 gcggcccgcc ctgatcgaga ccggccccgc caaggtcgcc ggcgagatcg ccgccggcgt
     5701 cacccgcgcc ggcctcgacg ccgccgacct cgcctggatc gtcgtcagcc acatccacct
     5761 cgaccacgca ggcggcgtcg gcgacctgct ccgcacgttc cccaacgcca ctgtggtcgt
     5821 ccaccccgcc ggcgccaggc acctggtcga ccccgagcgc ctgctggcca gctccgcgcg
     5881 ggtctacggc gcgctgatgg acaccgtcta cggcggcctc accccggtgg aggccgaccg
     5941 catcaaagct gccgaggacg gcgagaccct cgacctcggc ggccgccgcc tggaactgct
     6001 ccacgccccc gggcacgcca agcaccacat ggccgtgttc gaacccgacc tcggcgcgct
     6061 gttcaccggc gacggcgtcg gcgtgctgct cccgagcagt cccaccggcg tcctgcgccc
     6121 cgccacccct ccgcctgact tcgatcgcga cctggccgtt gccagcctgc gccgcatggc
     6181 caaactcgac cccgcccacg tcgtgctgac ccacttcggc cccatcgccc cacccgccga
     6241 acgcctcgcc gaggccgagg aacgcctgct gcgctggtgc gagaccgccg aagccgccgc
     6301 ccgcgagcac ggcccggagg tcgaccatat cgaggctgcc ctccgggaac gcttcgagca
     6361 ggaggagggc atcgccgccg acgccccaga aggcgagcag ggccgcctgc tgaacagctt
     6421 ccacagcaac gccgccggcc tcgcccgctg gatcgaggtc cgcgaccgcg accgcaaccg
     6481 caaccgcgac cagcggaggt aagcgcggga cgggccgcga tgccggcctc gagcaggctc
     6541 agagactcag agacgctcca gccgcgccgc cgcgtcgaag aactcgggat ccgcccgcac
     6601 gacccgggcg aacgcctccc gcgcacccgc ccggtcaccg gcccgctcgg cgaggtcacc
     6661 ctcggcgtag gcgagccgca ggtggtgcgc tgcgagctgc gccggccagc gcgcatgccg
     6721 tcggatcagc gccacaccgg ccgcgggctg gtccaggtcg ccgtacgcgg aggcagccac
     6781 gatcagcagc tccgcggcgg tatccgcctc catacgagtc cggtccgtcg agcggaacag
     6841 ctcgatcgcc tgctccggcc gtcccgtcgc ccgctcgata tccgccagca tcgcactcag
     6901 ctccaccccg cccgagatcc gctgcgccgc cgccagctcc gtccgcgccg ccttgtactc
     6961 ctccgcctga
//
LOCUS       DASWDC010000339         7403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_660849, whole genome shotgun sequence.
ACCESSION   DASWDC010000339 DASWDC010000000
VERSION     DASWDC010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660849"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VF995_10835"
     CDS             complement(<1..193)
                     /locus_tag="VF995_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344094.1"
                     /translation="MREPPGSGRDRGGSEVAWTIIGLLLSGVIAWGGIGWLLDHWLGT
                     SLFLPIGILVGAAGSFYLVI"
     gene            complement(262..663)
                     /locus_tag="VF995_10840"
     CDS             complement(262..663)
                     /locus_tag="VF995_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344095.1"
                     /translation="MDPADARIVRGAVLATVPVCAVVAVVAALQAGHKGLYGALLGSC
                     LAIAFFAVTIVAVSVAGRIDPLLMLPVALGTYLVKLIGIVAMLLLLRGSTAFNHTAFA
                     LATVIGACAFMTAEARMILRARIPYVTDTAM"
     gene            complement(664..1800)
                     /locus_tag="VF995_10845"
     CDS             complement(664..1800)
                     /locus_tag="VF995_10845"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX9344096.1"
                     /translation="MGDLRSYLLVCGATAMLAYLLTPAVRWVALRFGAVAHPNDRTVH
                     AEAMPTLGGLAMFLAFIGGLAVASSLPGLSQQLLTSSDAAGVAVGASLMVAIGIVDDL
                     KGLSAPVKLAGQIFAASVMTLAGVQVLYFWLPSLHNLQAGNIIILSPDLGVPLTVLLV
                     VVTVNAVNLVDGLDGLAAGLVAIGALAYFVYSYRTGAVGLIEASSPAPLLAALLFGAC
                     IGFLPHNFNPARIFMGDSGSLLLGTLLSGATITGIGRTTRPAAGDQFALLIPVIIPLL
                     VLALPFFDTFFAVGRRMFSGKGIMTADKNHLHHRLLEIGHSHRKAVLVLYGWSALLAG
                     AAVALAFTGARKVLPFFLIVVVCGVVGLLAPRWRFGRHRPASGA"
     gene            complement(1860..2480)
                     /locus_tag="VF995_10850"
     CDS             complement(1860..2480)
                     /locus_tag="VF995_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344097.1"
                     /translation="MTAETGAASSRTATPGDPFTVLFVCTGNICRSAMAEVFARRELA
                     SHPGLEVPLRFSSAGSYAMEGNRAVEASAVAAEARGASLERHRARQLTRRRVAAADLI
                     LCMEAEHRSPVLALDRAAGKRTFLLGTFARAIAELEREPPGTAPPASPAELAALAAER
                     LREQPGDGVDDPYGCPAAAHAACARRLDDLVSTVAASLLKTVRAPA"
     gene            complement(2470..3303)
                     /locus_tag="VF995_10855"
     CDS             complement(2470..3303)
                     /locus_tag="VF995_10855"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX9344098.1"
                     /translation="MSDSLSDDPRIIRIPKDEEQWDGLVQVAAEIIARGGLVVLPTDT
                     VYGVACDPFNASGTDALFKAKGRGRDLPLPVLIHTWRQALGLVEAVTEHAETLFAHYW
                     PGPLTVVLKESPGIGWDLGASRGTVALRMPKQGFLLKVIERTGPLAVTSANRSGEPTP
                     GKIPQVVEQLGDHVEAYFDDGWRMGRSPSTIVDLTGPKPMVLREGAIATAELEAVLEV
                     PLLDYSEEGGEELEMEDLDESGPFYPEGAIPSTEYVANLTKAEDPDPDPDPDPDPDDD
                     R"
     gene            complement(3333..4130)
                     /locus_tag="VF995_10860"
     CDS             complement(3333..4130)
                     /locus_tag="VF995_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX9344099.1"
                     /translation="MTARLAAAGCVSARAEARWLLEETGGEPEALEAMVARRAAGEPL
                     QYVIGWAPFGPLRIHVGPGVFVPRPETEGLADRAARHLRHAAPQERLAVDLCTGSGAI
                     ACFLAATVPGARVVATELDPGALVWAHRNCAAHGVELLAGDLDRPLPAALAERVRVLC
                     ANVPYVPSAAIATLPHDVQEHEPRAALDGGPDGLDVLRRVAATAPRWLAPGGVLFCEI
                     GEDQGAAATALFQAAGLTEVAVRPDLVGRDRVIRGQRPADLSNDRQT"
     gene            complement(4160..5239)
                     /gene="prfA"
                     /locus_tag="VF995_10865"
     CDS             complement(4160..5239)
                     /gene="prfA"
                     /locus_tag="VF995_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX9344100.1"
                     /translation="MFDRLDEIEQTYTEIERQLAAPDAFADQARFVALSKRHAELGEL
                     VRVYRAWKAANGDRTAARQLLREERTAEGRAMLDQELRDAQARASELEVELKLALAPK
                     DPNDDKDVIVEVRAGTGGDEAALFAGDLYRMYTRWAEQHGYKVEPLSRSESSLEGVKE
                     VIFAVHGKGAWSRLKYEAGVHRVQRVPVTESQGRIHTSAAGVNVLPEAEEIDVQIDPG
                     DLKIDVYRSTGPGGQSVNTTDSAVRITHFPSGVVVAMQDEKSQIQNREKAMRVLRARL
                     LERAQAEQQAQLAAERRSQVRSLDRSERIRTYNFPQDRVTDHRIGLSVGDLSGVLEGK
                     GLDRIIDELAARDHQAQLGEAGAPQ"
     gene            complement(5294..6343)
                     /locus_tag="VF995_10870"
     CDS             complement(5294..6343)
                     /locus_tag="VF995_10870"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX9344101.1"
                     /translation="MTDRTPETPASQARQPVYYGGQAVLEGVMMRGQDAWSIAVRRPQ
                     GEIYVEHHELAPLGKRVRLFRLPFFRGIGVLADSLMIGVRALSISGNQALGEEEQLSD
                     KQMGWSLAVGATFFSLLFILAPAAGTNWLGRHLPNNLAFNLIEGLARLAFFLGYILLI
                     SQFKDIRRVFQYHGAEHKAIHCYEAELPLSRDNVDRFPTLHVRCGTNFLLILFVVTLV
                     LFTLGFSLAGRPPLYVRVPLQLLAVPVIVGIAYEGIRLGAGRERSPLVRAIMKPGLWL
                     QMLTTKPPTHDQIEVAIRALESVLPETEREKVEPLPSQVVHRKPVASPTLAPTPAPAP
                     AVAAQPQPEDLPGGG"
     gene            complement(6388..6609)
                     /gene="rpmE"
                     /locus_tag="VF995_10875"
     CDS             complement(6388..6609)
                     /gene="rpmE"
                     /locus_tag="VF995_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX9344102.1"
                     /translation="MKQGIHPEYVVATVRCSCGETFQTRSTVPELRVDICSKCHPFYT
                     GKQKLVDTGGRVERFERRYGKRVRPGAES"
     gene            6899..>7403
                     /locus_tag="VF995_10880"
     CDS             6899..>7403
                     /locus_tag="VF995_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344103.1"
                     /translation="MRNRTNGTLAALLALVLTMGLAACQHRSAKAPPAAGAPSASAST
                     AAPGTTQASGQGSAVAASWPTYHRDLARAGVAQGPGRLGAVRRAWTSPQLDGAVYAEP
                     LLAAGGVVVATEANTVYYLEQATGRVAWHRNLGTPVPQSDLPCGNIDPVGITSTPVVD
                     PASGTLYT"
BASE COUNT         1094 a         2564 c         2731 g         1014 t
ORIGIN      
        1 ggatgacgag gtagaacgac ccagccgcac cgaccaggat gccgatcggc aggaacagcg
       61 aggtcccgag ccaatggtcc agcagccagc cgatgccgcc ccatgcgatc acccccgaaa
      121 gcagcagccc gatgatggtc caggcgacct cggaccctcc ccggtctcta ccactaccag
      181 gtggttctct catggggcgc cgactatacc aagggcacgc ggccccgagc gagcgccagc
      241 gcccgtccgc cgccgtgtct gtcacatagc cgtgtctgtc acatagggga tccgggcacg
      301 caggatcatg cgcgcctcgg cggtcatgaa ggcacaggcg ccgatcacgg tcgcgagcgc
      361 gaaggcggtg tggttgaagg cggtcgagcc gcgcagcagc agcaacatcg ccacgatgcc
      421 gatcagcttg accaggtagg tcccgagcgc gaccggcagc atcagcagcg ggtcgatgcg
      481 ccccgccacg ctgaccgcca cgatcgtcac cgcgaagaag gcgatcgcca ggcaactgcc
      541 gagcagcgcc ccatacaggc ccttgtggcc cgcctgcagc gccgcgacca cggcgacgac
      601 ggcgcagacc gggacagtgg cgagcaccgc cccgcgcacg atccgcgcgt cggccgggtc
      661 cactcaggct ccgctcgcgg gccggtggcg gccgaagcgc cagcgcggcg ccagcagccc
      721 gacgaccccg cagaccacca cgatcaggaa gaacggcagg accttgcgcg ccccggtgaa
      781 cgccagcgcc accgcggcgc ccgccagcag cgcactccac ccatacagca ccagcaccgc
      841 cttgcggtgg gagtggccga tctccagcag ccggtggtgc aggtggttct tgtcggcggt
      901 catgatcccc ttgccggaga acatccgccg ccccaccgcg aagaacgtgt cgaagaacgg
      961 caacgccagc accagcagcg ggatgatcac cgggatcaac agggcgaact ggtcaccggc
     1021 ggccgggcgg gtggtccgcc cgatcccggt gatggtcgcc cccgacagca gcgtgccgag
     1081 cagcagcgac cccgaatccc ccatgaagat ccgcgcgggg ttgaagttgt gcggcaggaa
     1141 cccgatgcag gcgccgaaca gcaacgcggc cagcagcggc gccgggctgg acgcctcgat
     1201 cagcccgaca gcgccggtgc ggtagctgta gacgaagtag gcgagcgcgc cgatcgccac
     1261 cagcccggcg gccaggccgt ccagcccgtc caccaggttg accgcgttga cggtcaccac
     1321 caccagcagc accgtcagcg gcacgcccag gtccggcgac aggatgatga tgttgccggc
     1381 ctgcaggttg tgcagcgacg gcagccagaa atacagcacc tgcaccccgg ccagggtcat
     1441 cacgctggcc gcgaagatct gcccggccag cttcactggc gcgctcaggc ccttgaggtc
     1501 gtcgacgatc ccgatcgcga ccatcagcga cgcgccgacc gccacccccg ccgcgtccga
     1561 cgaggtcagc agctgctgcg acaggcccgg cagcgacgag gcgaccgcca gcccgccgat
     1621 gaacgccagg aacatcgcca gcccgccgag cgtcggcatc gcctcggcgt ggacggtgcg
     1681 gtcgttggga tgcgcgaccg cgccgaagcg gagcgccacc cagcgcaccg ccggggtcag
     1741 caggtacgcc agcatcgcgg tcgccccgca gaccaggagg taggaacgta gatcgcccac
     1801 agcggggtga ggctatcacc ccaaggtcaa cgcaacgtca aacccgcatg tcaaacaggt
     1861 caagcgggcg cccggacggt cttgaggagg gaggcggcga cggtgctcac cagatcgtcg
     1921 agccgtctgg cgcaggcggc gtgggcggcg gcgggacacc cgtaggggtc gtccacgccg
     1981 tcgccgggct gctcgcgcag ccgttcggcg gcgagggcgg ccagctcagc cggcgaggcc
     2041 ggcggggccg tgccgggcgg ttcccgctcc agctcggcga tcgcgcgtgc gaacgtcccc
     2101 agcaggaacg tccgcttgcc cgcggcccgg tccaatgcca gcaccggcga ccggtgctcg
     2161 gcctccatac agagaatcag gtccgcggcc gcgacccggc ggcgggtcag ctgccgcgcc
     2221 cggtggcgct ccagcgaggc gccccgcgcc tccgccgcga ccgccgaggc ttcaaccgcc
     2281 cggttgccct ccatcgcgta gctgccggcg gagctgaacc gcagcggcac ctccagcccg
     2341 gggtggctgg ccagctccct ccgcgcgaac acctcggcca tcgctgacct gcagatgttg
     2401 ccggtgcaga cgaacagcac cgtgaagggg tcgcctggcg tcgcggtccg ggaggaggcc
     2461 gcaccagtct cagcggtcat cgtccgggtc cgggtccggg tccgggtccg ggtctgggtc
     2521 ttcggccttg gtgaggttgg cgacatactc ggtgctcggg atggcgccct cggggtagaa
     2581 gggcccggat tcgtcgaggt cctccatctc cagctcctcg ccgccctcct cgctgtagtc
     2641 gagcagcggg acttcgagga cggcttcgag ctcggcggtg gcgatggcgc cttcacgcag
     2701 gaccatgggc ttggggccgg tcaggtcgac gatggtggag ggtgagcggc ccatcctcca
     2761 gccgtcgtcg aagtaggctt cgacgtggtc gccgagctgt tcgacgacct gggggatctt
     2821 gccgggggtc ggttcgccgc tgcggttggc gctggtgacg gcgagcgggc cggtgcgctc
     2881 gatgaccttg agaaggaagc cctgcttggg catgcgcagc gccaccgtgc cgcgcgaggc
     2941 gccgagatcc cagccgatgc cgggcgactc cttgagcacg acggtgagcg gtccgggcca
     3001 gtagtgcgcg aacagcgtct cggcgtgctc ggtgacggcc tcgaccaggc cgagggcctg
     3061 gcgccaggtg tggatcagca ccggcagcgg caggtcccgg ccgcgtccct tggccttgaa
     3121 gagggcgtcg gtgccggagg cgttgaaggg gtcgcaggcg acgccgtaga cggtgtcggt
     3181 gggcagcacc accaggccgc cgcgcgcgat gatctcggcg gcgacctgga ccaggccgtc
     3241 ccattgttcc tcgtccttgg ggatccggat gatccgcggg tcgtcgctca agctgtcgct
     3301 catgctcgct ccttagatca gtgcttcagg tctcaggtct ggcggtcgtt gctcaggtct
     3361 gccggtcgtt ggccgcggat gactcggtcg cggccgacca ggtcgggccg cacggcgacc
     3421 tcggtcaggc cggccgcctg gaacagcgcg gttgcggcgg cgccctgatc ttccccgatc
     3481 tcgcagaaga gcacaccgcc gggggcgagc cagcggggcg ctgtggcggc gacccggcgc
     3541 aggacgtcga ggccgtcggg gccgccgtcg agggcggcgc gcggttcgtg ctcctggacg
     3601 tcgtggggca gggtggcgat cgcggcgctg ggcacatagg ggacgttggc gcacagcacc
     3661 cgcacccgct cggccagcgc ggcgggcagc ggccggtcga ggtcgccagc gaggagttcc
     3721 acgccgtgcg cggcgcagtt gcggtgtgcc cagacgagcg cgcccgggtc cagctcggtg
     3781 gcgacgaccc gcgcgccagg gacggtggcg gccaggaagc aggcgatcgc gccggagccg
     3841 gtgcagaggt ccaccgcgag gcgctcctgt ggagcggcgt ggcggaggtg gcgggcggcg
     3901 cggtcggcga ggccctcggt ttcgggccgg gggacgaaca cgccgggccc gacgtggatc
     3961 cgcagcgggc cgaagggcgc ccagccaatc acgtactgga gcggctcccc ggccgcgcgg
     4021 cgcgcgacca tcgcctccaa ggcctccggc tcgccgccgg tctcctccag cagccagcgg
     4081 gcctcggccc gggccgagac gcacccggcc gccgcgagcc gcgcggtcac cgcggcgagc
     4141 ggactggtgc gggaagttct cattggggtg cccccgcctc gcccagctgg gcctggtggt
     4201 cgcgggcggc gagctcgtcg atgatgcggt cgaggccctt gccctccagc acgccggaga
     4261 ggtcgccgac tgagaggccg atgcggtggt cggtgacgcg gtcctggggg aagttgtagg
     4321 tgcggatccg ctcggagcgg tccagggagc ggacctggga gcggcgttcg gcggcgagct
     4381 gagcctgctg ctcggcctgg gcgcgttcca ggaggcgggc gcggagcacg cgcatggcct
     4441 tctcgcggtt ctggatctgc gacttctcgt cctgcatcgc caccacgacg ccggatggga
     4501 agtgggtgat gcggaccgcc gagtcggtgg tgttgaccga ctggccgccc gggccggtgg
     4561 agcggtagac gtcgatcttg aggtcgcccg ggtcgatctg gacgtcgatc tcctcggctt
     4621 cggggagcac gttgaccccg gcggcggagg tgtggatgcg gccctgcgac tcggtgacgg
     4681 ggacccgctg gacacggtgg acgccggctt cgtacttgag gcgcgaccag gcacccttgc
     4741 cgtggacggc gaagatgacc tctttgacgc cttccaggct tgattcgctg cgggacagcg
     4801 gctccacctt gtagccgtgc tgctcggccc agcgggtgta catccggtag aggtcgccgg
     4861 cgaacagggc ggcctcgtcg ccgccggtgc cggcgcggac ctcgacgatc acgtccttgt
     4921 cgtcgttggg gtctttcggc gcgagcgcca gcttcagctc cacttccagc tcgctcgcgc
     4981 gcgcctgggc gtcgcgcagc tcctggtcga gcatggcgcg gccctcggcg gtgcgctcct
     5041 cgcgcagcag ctggcgcgcg gcggtgcggt cgccgttggc agccttccag gcgcggtaga
     5101 cgcggaccag ctcgcccagc tcggcgtggc gcttggaaag cgccacgaag cgggcctggt
     5161 cggcgaacgc gtcgggggcg gcgagctgcc gctcgatctc ggtgtaggtc tgttcgatct
     5221 cgtccaagcg gtcgaacacg ggaactccag ctcctcagga tcttcggaag gggggtggac
     5281 acgcggaagc gtgctatccc ccgccgggca gatcctcggg ttgcggctgc gccgcgaccg
     5341 ccggcgccgg cgccggggtc ggggccaggg tcggcgaggc gaccggcttg cggtggacga
     5401 cctgcgacgg gagcggctcg accttctccc gttcggtttc ggggaggacc gactcgagcg
     5461 cgcggatcgc gacctcgatc tggtcgtggg tgggcggctt ggtggtcagc atctgcagcc
     5521 acaggcccgg tttcatgatc gcccggacca ggggcgagcg ctcccggccg gcgccgaggc
     5581 ggatgccctc gtaggcgatg ccgacgatca ccgggaccgc cagcagctgc agcggcaccc
     5641 gaacgtacag cggcgggcgg ccggccaggc tgaagccgag ggtgaacagc accagggtga
     5701 ccacgaacag gatcagcagg aagttggtgc cgcagcggac gtgcagggtg gggaagcggt
     5761 cgacgttgtc gcggctgagc ggcagctccg cctcgtagca gtggatcgcc ttgtgctcgg
     5821 cgccgtggta ctggaagacc cggcggatat ccttgaactg cgagatcagc aggatgtagc
     5881 cgaggaagaa ggcgagccgc gccagcccct cgatcaggtt gaaggcgagg ttgttgggca
     5941 ggtggcggcc gagccagttg gtgcccgcgg cgggcgcgag gatgaacagc agcgagaaga
     6001 aggtcgcgcc gaccgccagc gaccagccca tctgcttgtc ggagagctgc tcctcctcgc
     6061 cgagggcctg gttgccggag atcgacaggg cccgcacgcc aatcatcagc gagtcggcca
     6121 gcacgccgat gccgcggaag aacggcagcc ggaacagccg cacgcgcttg ccgagggggg
     6181 ccaactcgtg gtgttcgacg tagatctcgc cctggggccg gcggaccgcg atcgaccagg
     6241 cgtcctggcc gcgcatcatc acgccttcca ggacggcctg gccgccgtag tagaccggtt
     6301 gccgcgcctg ggacgcgggc gtctcaggcg tccggtcggt cacttgctca tctccagatt
     6361 atccagcttc gcgaggtcca gcggcgctca ggactcggcg cccggccgga cccgcttgcc
     6421 gtagcggcgc tcgaagcgct cgacccggcc gccggtgtcg accagcttct gcttgcctgt
     6481 gtagaacggg tggcacttcg agcagatgtc cacccgcagc tccggcacgg tcgagcgggt
     6541 ctggaaggtc tcaccgcagg aacagcgcac ggtggcgacc acgtactcgg ggtggatgcc
     6601 ctgcttcatg gttggtgaca ctcctcactg catcaaaacg tcacttcagg taggtgatgg
     6661 gaggatcccc gggcggcgcc tccgcgccat ctgaacgccc aggccgaacg aacttcggta
     6721 gatcctacac cagcgtcaac cgcacgagac cggcccgcat tccagcccgg cgtatcgccc
     6781 ggcgtatcgg gggcccccgc ggccgccatg ggagcggccc gcggcgcctc cggccgcggg
     6841 accgcgcccc cgccacccga ctggagatat tccacgccga gggttagcgt cattgatcat
     6901 gcgcaaccgc acaaacggca cgctcgccgc gctcctcgcc ttggtgctga cgatggggct
     6961 ggcggcctgc cagcatagga gcgccaaggc gccgcccgcc gccggggcgc cctcggcttc
     7021 tgcctccacc gcggcgcctg ggacaaccca ggcatctggg cagggctcgg cggtggccgc
     7081 ctcctggccg acctatcacc gcgacctcgc ccgtgcgggc gtggcgcagg ggcctgggcg
     7141 gctcggggcg gtgcggcgcg cgtggacctc gccgcagctg gacggggcgg tctatgcgga
     7201 gccgctgctc gccgcgggcg gcgtggtcgt cgccaccgag gcgaacacgg tctattacct
     7261 ggagcaggcc accggccggg tcgcttggca ccggaacctg ggcacgccgg tgccgcagag
     7321 cgacctgccg tgcggcaata tcgacccggt tgggatcacc tcgacgccgg tggtcgaccc
     7381 ggcgagcggc accctgtaca cgg
//
LOCUS       DASWDC010000340         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1145613, whole genome shotgun sequence.
ACCESSION   DASWDC010000340 DASWDC010000000
VERSION     DASWDC010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145613"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..87)
                     /locus_tag="VF995_10885"
     CDS             complement(<1..87)
                     /locus_tag="VF995_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009030042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HEX9344104.1"
                     /translation="MAAVCDICGKHPGFGMSVSFSHKRNPRRW"
     gene            242..925
                     /locus_tag="VF995_10890"
     CDS             242..925
                     /locus_tag="VF995_10890"
                     /inference="COORDINATES: protein motif:HMM:NF039470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HEX9344105.1"
                     /translation="MYQQDYLMRQLEQAAQALGRILRKALGGDPAAALAMFDEAYQPL
                     LGVGARVVSTLNETQLLTLLTSGSSPDLRRVASVLEVLKTEGDLYQQAGQGREAAKRY
                     RRALILAGFLAARSEEALDRDVATALVGHAAGLDLGLSGTDRLQLAQVLEALGRYADA
                     EDALFEAIDQEPANTALVDAGIAFYQRLLALSPEHLDAGGLPAVEVRESLAELLRRQV
                     LPDDEDEEP"
     gene            complement(961..1407)
                     /locus_tag="VF995_10895"
     CDS             complement(961..1407)
                     /locus_tag="VF995_10895"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9344106.1"
                     /translation="MASRKDALVARVLLVNDGKVLAAHHRHPGSGDGSGDADFWCLPG
                     GKAKPGERLEDAARRELHEETGLAADLAGVIWLQDLPSQGCFVVVFAAWLPEGHSGAL
                     APPQPGDKHLVETAWRTVPDLLAGDFRPSALLKLLPLGNLPELPRP"
     gene            complement(1410..2339)
                     /gene="thiD"
                     /locus_tag="VF995_10900"
     CDS             complement(1410..2339)
                     /gene="thiD"
                     /locus_tag="VF995_10900"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018102415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX9344107.1"
                     /translation="MPVPSPRRVLTIAGSDSGGGAGIQADLKTFLAHGAHGMTAITAV
                     TVQNSVGVQGFYELPPQAVFEQIESVATDIGVEAAKTGMLASAPIISAVADACERFAV
                     PNLVVDPVAVSKHGDPLLAADAVATLRTRLLPLATVVTPNLGEAALLAGSGDAAGAPG
                     AAGRLAPIADRDGMRAAAAALHALGPRYVLVKGGHLPGDDAADLLYDGRQELWLSAPR
                     LATPHTHGTGCTLSAAIAANLAEGIDVPGAVRLAKQYVTGAIAGSFPLGKGVGPTDHA
                     WRFRAAGLLRPGGPSNPAAADAAWPYGPNPYEG"
BASE COUNT          338 a          884 c          962 g          329 t
ORIGIN      
        1 ccagcgccgg gggttgcgct tgtgcgagaa gctgacggac atgccgaacc ccgggtgctt
       61 gccgcagatg tcacagaccg cagccatgaa ccaagccttt catacacgtg cacgtgagag
      121 aatgcggttc gccttcgtca acgtccgcgt cgaagccgcc ggggtacggt agcaccgcgc
      181 cgcccatggg gcacatcagc ctggcaccct gacagcgcag ccagccggcg ggaggacgcc
      241 tttgtaccag caagattacc tgatgcggca gctcgagcag gcggcgcagg cgctcggccg
      301 gatcctgcgc aaggcgctgg gcggcgaccc ggcggcggcg ctggccatgt tcgacgaggc
      361 ctatcagccg ctgctgggcg tcggggcgcg ggtggtctcg accctgaacg agacgcagct
      421 gctcaccctg ctgacctccg ggtccagccc ggacctgcgg cgggtcgcct cggtgctgga
      481 ggtgctcaag accgagggcg acctctacca gcaggccgga caggggcgag aggccgccaa
      541 gcgctaccgc cgcgccctga tactggccgg gttcctggcc gcccgctcgg aggaggccct
      601 ggaccgcgac gtcgccaccg ccctggtcgg ccacgccgcc gggctggacc tcggcctcag
      661 cggcactgac cggttgcagc tcgcccaggt cctcgaggcc ctcggccgct acgccgacgc
      721 cgaggacgcc ctgttcgagg cgatcgacca ggagcccgcc aacaccgcgc tggtggacgc
      781 gggcatcgcc ttctaccaac gcctgctcgc cctttccccg gagcacctcg acgctggtgg
      841 tctgcccgct gtcgaggtcc gcgagtcgct ggcggagctg ctccgcaggc aggtcctccc
      901 cgacgacgag gacgaggagc cctgagcgtg aagcgcggct gggccccagg cccggtggcc
      961 ctatggtcgc ggcagttccg ggaggttgcc gagcggcagc agcttgagca gcgccgaggg
     1021 gcggaagtcg ccggcgagga ggtcggggac ggtacgccag gcggtctcca ccaggtgctt
     1081 gtcgcccggc tgcggtggcg ccaaggcgcc ggagtgaccc tcggggagcc acgcggcgaa
     1141 gaccaccacg aagcagccct ggctggggag gtcctgcagc cagatcacgc cggcgaggtc
     1201 ggcggcgagg ccggtctcct cgtgcagctc gcggcgggcg gcgtcctcca ggcgctcgcc
     1261 gggcttggct ttgccgccgg ggaggcacca gaagtcggcg tcgccggagc cgtcgccgga
     1321 gccggggtgg cggtggtggg cggcgaggac cttgccgtcg ttgaccagca gcacgcgggc
     1381 gaccagcgcg tccttgcgac ttgccatctt cagccctcgt aggggttggg gccgtacggc
     1441 catgcggcgt cggcggcagc ggggttgctc gggccgccgg ggcgcagcag gccggcagcg
     1501 cggaaacgcc aggcgtggtc ggtggggcca acgcccttgc cgagcgggaa cgacccggcg
     1561 atggcgccgg tcacgtactg tttggcgagc cgcaccgccc cgggcacgtc gatgccctcg
     1621 gcgaggttgg cggcgatcgc cgccgacagg gtgcagccgg tcccgtgagt atgcggcgtg
     1681 gccagcctcg gggcgctgag ccacagctcc tggcggccgt cgtagagcag atcggcggcg
     1741 tcatcgccgg gcaggtggcc gcctttgacc aggacgtagc gcggcccgag ggcgtgcagc
     1801 gcggcggcgg cggcgcgcat gccgtcgcga tcggcgatcg gcgcgaggcg gccggcagcg
     1861 ccgggagcgc ctgcggcgtc gccgctgccg gcgaggaggg cggcctcgcc gaggttgggg
     1921 gtgaccacgg tggcgagcgg cagcaggcgg gttcgcagcg tcgcgaccgc gtcggccgcg
     1981 agcagcgggt cgccgtgctt ggaaacggcc accggatcga ccaccaggtt ggggacggcg
     2041 aagcgctcgc aggcgtcggc gaccgccgaa atgatcggcg cggaggccag catcccggtc
     2101 ttggcggcct caacgccgat atcggtggcg accgactcga tctgctcgaa gacggcttga
     2161 ggcggcagct catagaaccc ctgcacgccg accgagttct gcacggtcac ggcggtgatc
     2221 gccgtcatcc catgggcgcc gtgcgccagg aaggtcttga ggtcggcctg aatgcccgca
     2281 ccgccgcccg agtccgaccc ggcgatcgtg agcacgcgcc ttgggcttgg caccggcatc
     2341 gtggttggtc cctccgtttc ccgttcggct cgctggccca gacctgctgc ggccccaggc
     2401 tacccgcgcc gggcccgccg cagggcggcc cggcgccgct ccggcggccg gctcggcgct
     2461 ccggcagcga cgccagcctc agcggccgtg gcgcaggcgc agcatgctcg aca
//
LOCUS       DASWDC010000341         4060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_411454, whole genome shotgun sequence.
ACCESSION   DASWDC010000341 DASWDC010000000
VERSION     DASWDC010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411454"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VF995_10905"
     CDS             complement(<1..738)
                     /locus_tag="VF995_10905"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX9344108.1"
                     /translation="MSAGAVALATVLALAAPATASVRARLAWAPAMATPTAMIRIGHF
                     SPDESYVDVYAVSLNRRQFFPNVFYKTVSAYWPVAAGPFTYEVRPAGATPSDPPALRV
                     SAALTPGHSYTVAAYGHKGALRGLLLDDTFTPASPGQGKLRVLDTLLDDGPLDVLLGP
                     GGGLGGQALASGVMLGTATPYRQLPAGTYPVAVRHAGNAGGGAGSGVLGHGTVAVRAG
                     TVTTLAVVGGGGQPDGLVPFADAAGSAA"
     gene            990..2015
                     /locus_tag="VF995_10910"
     CDS             990..2015
                     /locus_tag="VF995_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX9344109.1"
                     /translation="MPPPKMPPPKLAFRWRALTTALLLSGLLAGPGALVAAAAPSPAP
                     PAGGLPRPQAIQASAAALRAKLDAQNTRLEVLAEDLDEAYTSGAELLAAAAATAQRRQ
                     RLAVGLRAASADPDALRQLQADKARLDRLAALMSARVVAQAQKAERMYRARAEAATLA
                     ATMTRELHTMDARMAALLDSDQRRLEAERRAAFTRYLAFARARAGGGTLAATGPASPL
                     ARHAVQVALAQLGVPYEYGAAGPTRFDCSGLTSFAYRAAGLTIPRTAAWQFAADAADQ
                     FVAPTALRPGDLVFYATDPADPATIHHVGMYLGKGLMVEAPHTGAVVRVASIWRPDYA
                     GAVRPAP"
     gene            2117..4012
                     /locus_tag="VF995_10915"
     CDS             2117..4012
                     /locus_tag="VF995_10915"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9344110.1"
                     /translation="MAMVDSDDLRRWRELGQQLRVDSVRASAAAESGHPTSSMSAADL
                     MAVLLDKYFRYDFDNPRNPGNDHLIFSKGHASPLLYALYKAAGAISDEELLSFRRFGS
                     RLEGHPTPRLPFVDVATGSLGQGLPVGVGVALAGAELDRLPYRVWVLCGDSELAEGSI
                     WEAFQHAGFSKLRNLTAIIDVNRLGQRGETMLGWDTATYARRARAFGWHAIEIDGHDV
                     AAVADAYAEAVATADEPVAIFARTVKGKGVKSVENVNGAHGKPLDDPTAAIAELGGER
                     NLVITPGKPRASGEAHGFEVEPSPPDLPTWPVGEKVATRKAYGEALAALGASRADVVA
                     LDGEVSNSTYAELFAKAHPRRYFEMYIAEQQMIAAAVGMQVRGWRPFASTFAAFLTRA
                     HDFIRMAAVSRASIRLMGSHAGISIGEDGPSQMGLEDLAMLRAVHGSTVLYPCDANQT
                     ADLVARMADLDGVSYLRTTRGGTAVVYEPGREFPVGGSVVLRSSPQDQVTIVSAGITL
                     HEALAAAERLDGEGLHVRVIDCYSVKPIDAATLRQAATETGRVLTVEDHWPQGGLGDA
                     VLEVFADAAPAPLLLKLAVPDLPGSGTPQELLHQAGLDADGIATTARRLAEAKAGNGA
                     GPGPEAR"
BASE COUNT          558 a         1597 c         1394 g          511 t
ORIGIN      
        1 ggcggcgctg ccggctgcgt cggcgaaggg caccagcccg tccggctggc cgccgccgcc
       61 gaccaccgcc agggtggtca ccgtcccggc cctgaccgca acggtgccgt ggcccagcac
      121 ccccgagccc gcgccgccgc ccgcgttacc cgcgtggcgc accgccaccg ggtaggtgcc
      181 ggccgggagt tggcggtagg gggttgccgt gccgagcatg accccgctgg ccagcgcctg
      241 accaccgagg ccgcccccgg ggccgaggag gacgtcaagc ggcccgtcgt ccagcagggt
      301 gtcaagcacc cgcaacttgc cctgccccgg actggcaggc gtgaaggtgt cgtcgagcag
      361 cagcccgcgg agggcgccct tgtgaccgta ggccgccacg gtgtaggagt ggccgggggt
      421 gagggcggcg ctgacccgca gcgccggcgg gtcgctcggc gtcgcgccgg ccggtcgcac
      481 ctcataggtg aacggcccgg ccgccaccgg ccagtaggcc gagaccgtct tgtagaacac
      541 gttcgggaag aactgtcgcc ggttcaggct caccgcgtag acgtccacgt acgactcgtc
      601 cggcgagaaa tgcccgatgc gaatcatcgc ggtcggcgtg gccatggcag gtgcccaggc
      661 caagcgggcg cgaacgctcg cggtcgccgg tgcggccagc gccagtacgg tggcgagcgc
      721 gaccgcccca gcgctcactg gcgagatggt ccgtcgcacc agccgtcgca gcaccgactg
      781 ctcctctcgg atcgccgttc ctgccgtcca cgtcacttct gcgttcgtgg gcggccccga
      841 tccggtgctc cgtagctcgc gtctacggta gatcgcggag tttccactac gcccagatcc
      901 gcgccgcgcc ctcccgacga atgccctggc gacggcgacc acgcaagacc aagcagcagc
      961 atccggaacg gacaacggac ggagtctcga tgccaccacc caagatgcca ccacccaagc
     1021 tggccttccg ctggcgcgcc ctgaccacgg ccctcctgct cagcgggctg ctggccggtc
     1081 cgggcgcgct ggtcgccgcg gcagccccca gtccggcccc tccggctggc gggctgccgc
     1141 ggcctcaggc catccaggcg agcgccgcgg cgctgcgagc caagctcgac gcccagaaca
     1201 cccgcttgga ggtcctcgcc gaggaccttg acgaggcgta caccagcggc gccgagctgc
     1261 tggccgccgc cgccgcgacg gcccagcgcc gccagcggct ggcggtgggg ctccgcgccg
     1321 ccagcgctga cccagacgcc ctgcggcaac tccaggctga caaggcgaga ctcgaccggc
     1381 tcgccgccct gatgtccgcc cgggtggtcg cccaggcgca gaaggccgag cggatgtacc
     1441 gcgcacgcgc cgaggccgcc acgctggccg cgaccatgac ccgcgagctg cacaccatgg
     1501 acgcccgcat ggccgcgctg ctcgacagcg accagcgccg gctggaggcc gagcgccgcg
     1561 ccgccttcac ccgctacttg gccttcgccc gtgcccgggc cggcggcggg accctggcag
     1621 ccacggggcc ggccagcccg ctcgcccgcc acgccgtcca ggtcgccctg gcccagctcg
     1681 gggtccccta cgagtacggc gcggccgggc cgacccgctt tgactgctct ggcctgacca
     1741 gcttcgccta tcgcgccgcc gggctcacca tcccgcgaac ggcagcctgg cagttcgccg
     1801 ccgacgccgc cgaccagttc gtcgccccca ccgcgctgcg ccccggcgac ctggtcttct
     1861 acgccactga cccggccgac ccggcgacca tccaccacgt cggcatgtac ctcggcaagg
     1921 gcctgatggt cgaggcaccc cacaccggcg cggtggtccg cgtcgcctcg atctggcgcc
     1981 ccgactacgc cggcgcggtc cggccggccc cgtagggctg gcaggccgcg gcctcctccc
     2041 ggttccctgg tttggcgttc cgcagcgggt gggagccatc acctgaactg ttctcggatg
     2101 gaacgagggg cctgcgatgg cgatggttga cagcgacgac ctgcggcgtt ggcgggaact
     2161 cgggcaacag ctccgggtcg attcggtgcg ggcgagcgcg gcggcggagt cgggccaccc
     2221 gacctcgtcg atgtcggccg ccgacctcat ggccgtgctg ctcgacaagt acttccgcta
     2281 cgacttcgac aaccctcgca accccggcaa cgaccacctg atcttttcca aggggcatgc
     2341 ctcgccgctg ctgtacgcgc tgtacaaggc cgccggggcg atcagcgacg aggagctgct
     2401 gagcttccgc aggttcggca gccgcctgga aggacacccc accccccggc tgccgttcgt
     2461 cgacgtggcc accggctcgc tcggccaggg cctgccggtc ggggtcggcg tggcgctggc
     2521 cggcgccgag cttgaccgcc tgccctaccg ggtctgggtg ctttgcggcg acagtgaact
     2581 ggcggagggg tcgatctggg aggccttcca gcacgccggc ttctccaagc tccgcaacct
     2641 caccgcgatc atcgacgtga accgcctcgg ccagcggggc gagaccatgc tcggctggga
     2701 caccgcgacc tacgcgcgcc gcgcccgcgc cttcggctgg cacgcgatcg agatcgacgg
     2761 gcacgacgtc gccgctgtcg ctgacgccta cgccgaagcg gtcgccaccg ccgacgagcc
     2821 ggtggcgatc ttcgcccgca ccgtcaaggg caagggcgtc aagtcggtcg agaacgtgaa
     2881 cggcgcccac ggcaagccgc tcgacgaccc caccgccgcc atcgccgaac tcggcggcga
     2941 gcgcaacctc gtgatcaccc cgggcaagcc gcgcgccagc ggcgaggcgc acggtttcga
     3001 ggtcgaaccc agcccgcccg acctgcccac ctggccggtg ggggagaagg tcgccacccg
     3061 caaggcctac ggagaggcgc tggcggcgct tggggcgagc cgcgccgacg tggtcgccct
     3121 cgacggcgag gtctccaact ccacctacgc cgagctgttc gccaaggccc acccgcggcg
     3181 ctacttcgag atgtacatcg ccgagcagca gatgatcgcc gccgccgtcg gcatgcaggt
     3241 gcgcggctgg cggccgttcg cctccacctt cgcggccttc ctgacccgtg cccacgactt
     3301 catccgcatg gccgccgtga gccgcgcgag catccggctg atgggctccc acgcgggcat
     3361 ctccatcggt gaggacggcc cctcgcagat gggcctggag gacctcgcca tgctccgggc
     3421 ggtgcacggc agcaccgtgc tgtacccctg cgacgccaac cagaccgccg acctggtcgc
     3481 ccgcatggcc gacctcgacg gcgtcagcta cctgcgcacc acccgcggcg gcaccgcggt
     3541 cgtctatgag ccgggccgcg agttcccggt gggcggcagc gtggtgctgc gctcctcgcc
     3601 gcaggaccag gtcaccatcg tgagcgccgg gatcactctg cacgaggcgc tcgccgccgc
     3661 cgagcggctg gacggcgagg gcctgcacgt ccgcgtgatc gactgctact cggtcaagcc
     3721 gatcgacgcc gcgaccctgc gccaggcggc caccgagacc ggccgcgtcc tcaccgtcga
     3781 ggaccactgg ccacagggcg gcctcggcga cgccgtcctg gaggtcttcg ccgacgccgc
     3841 acccgcgccg ctgctgctga agctcgccgt gcccgacctg cccggctcag gcaccccgca
     3901 ggagctgctc caccaggccg gcctggacgc cgacggcatc gccaccaccg cccgccgcct
     3961 cgccgaggcc aaggccggca acggcgccgg acccggaccc gaggcccgct gaggccgggc
     4021 ggtccggcgc cagcgcgttc cggcgccggc gggacccacg
//
LOCUS       DASWDC010000342         8840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1562684, whole genome shotgun sequence.
ACCESSION   DASWDC010000342 DASWDC010000000
VERSION     DASWDC010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562684"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VF995_10920"
     CDS             complement(<1..308)
                     /locus_tag="VF995_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344111.1"
                     /translation="MSVRVRVWSGIACLVAVVAIGVAVGRGPAPDRVDGGVRQHLHSW
                     LERRSFRDLLGEVAAERRAGHGTYVTAALPLLAAGVVAAGTALTAARRGRPRPPARTW
                     A"
     gene            361..1437
                     /locus_tag="VF995_10925"
     CDS             361..1437
                     /locus_tag="VF995_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344112.1"
                     /translation="MTIRRRSHRQQLRLPVRLRTAAVAALVVALTAGCGSGSGRRQPA
                     AQMVTPPKPAPRSGPPPVLAVHFDRGKYPLLGSREADLSKAAEAFNAVLVSYGDQQLV
                     EQARARGLKVYLGFDEHQEFAGGKDISADVRDLVRFASANRDQIAGIRVADRVNEGLT
                     PAQTLGYLRATGGVLHQRLPDVPVIVDVSDWQLTCDLLGQNPCGKARSAAYRYQTNAG
                     LLQIYHSGYVDGFMLADGLARDDTDCGGCTAQASDATAQTAAWKRARALFPHPFLLFA
                     RVAPLSFPQAHYPADAVQAALAVSVYETIPLQQGADGVDLWAWSRHFDGTVRTFLDKD
                     GASNPLWQQFVTLATAMHAQARPS"
     gene            complement(1477..2232)
                     /locus_tag="VF995_10930"
     CDS             complement(1477..2232)
                     /locus_tag="VF995_10930"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX9344113.1"
                     /translation="MSTFISRSRSSLSGRLGIPPGVPRRALDLLVAGLALVVLSPVFG
                     VVALLVRLSSPGAVLFRQSRVGQGGVPFTLLKFRTMRAGASGPNVTTADDLRLTPLGK
                     LLRLAGLDELPQLLNILRGEMTLVGPRPETPDLASRYLPGCEDVFRYRPGLTGPAAVR
                     LRDRDELRSVDEDLEEYYLQVVVPAKVTVDLDFLAAPTLRRTCGVMGETLLYIVTGGR
                     TRRTQPLGTVTLARPPVAFTEPARIFSRTLSRV"
     gene            complement(2274..3641)
                     /locus_tag="VF995_10935"
     CDS             complement(2274..3641)
                     /locus_tag="VF995_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9344114.1"
                     /translation="MNANRVPAGTRVPAWPSDLASGGQVVTDAAGGLPPQPLPSQVKV
                     LHVITKFWAGAGGNTLLTVLGADPGRYETWVAGCDGGPLWERAEQAGIRTVRLKRFRE
                     TISPFNDLSVLVQLVRLIRRERPAIVHTHSAKAGFLGRLAAWLCHTPVILHTFHGLPF
                     HDFMSKRRKRVYLLLDRLVRPFTDEFLSVAPRVAREAVEQRIAPPGSVTVVPSAVELD
                     QIPTMPDPAARAEFGIAPHTPLIGTVGRLEYQKAPLDFVRMAATVAASRPDACFVMVG
                     EGPLLDDAKAEAARLGVEVRFTGFRADAPRIASAFDVFVISSLYEGLGRALTEAVASG
                     RPVVATAVNGVPDLIVPGATGLLATPGDPASLAAGVLWLLDHPGEASRMGDAGSRWVR
                     WMFDPALMCALIDQTYCRLLGIPGPAATSPPPTVLPHPAPAAARARVRARTAATSGGP
                     ERRSR"
     gene            complement(3638..4819)
                     /locus_tag="VF995_10940"
     CDS             complement(3638..4819)
                     /locus_tag="VF995_10940"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX9344115.1"
                     /translation="MAIEFEIPFARAEVCAEAHEAAAQVLRSGWLTTGPEVAAFEREF
                     AEWVHADHAVAVASCTAAIELALRALRLPRGAKVLTSTMTFCGAVHAIVHAGLVPVLA
                     DVDPVTLSSDAAACAAAARRAGGVDAMVITHYAGLPSPVEELAEAVGLPLGRVVEDAA
                     HALGTWHGARPVGSCSAATCFSFYATKNLPIGEGGMVTTADQEVADHLRRTRLHGMSK
                     DAWKRYLPGEAWRYSVEVAGLKANMTDLQAAIGRAQLRHLAAWQERRALLARRYDDGL
                     AAVGGLGLPARPEWGRHAWHIYVVQVEAEYGLSRDELIAALAERGVGTSVHFIPVHHF
                     PYFQQLLDPEQLGTHPGADAAFERIVSLPLFQGMALDDVDRVCETLASLRRHPAKAAL
                     R"
     gene            complement(4859..5911)
                     /locus_tag="VF995_10945"
     CDS             complement(4859..5911)
                     /locus_tag="VF995_10945"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9344116.1"
                     /translation="MNILVTGGAGYIGSRLLHMLASDGSLPVRRVRVLDNMREEKFPS
                     LMNLPDGVSYEFVLGDIRNEGDVQAALGDDTDVVVHLAALCNATISFDRRDATEEINY
                     GGTVNVVRAARMAPSVKRFIYASSTSVYGPTSGIVDEESPCFPASPYAEFKLKGEQDV
                     LGLSTDTGGRLHPTVLRFATVYGYSPGLRVHTVVNIFAFRAAVGAPIEIYGAGNQLRP
                     FIHVTDVSRAIAFCLAGDQTHDGIFNVVGENASVNQIVALLRSHFPKVQITHADKEIL
                     NQISYEVDGSRLRALGFGLAHTLEDGIAEYAHLYGSFGRLPLEFGIGNGNGNGHANSH
                     GNSQGTAPVPSAVTTG"
     gene            6128..6289
                     /locus_tag="VF995_10950"
     CDS             6128..6289
                     /locus_tag="VF995_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344117.1"
                     /translation="MADEELTTSSPRPVDTPLDAEAQAAGEPLADDYSDAERDEVAAR
                     LASLGYIDE"
     gene            6315..7919
                     /locus_tag="VF995_10955"
     CDS             6315..7919
                     /locus_tag="VF995_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9344118.1"
                     /translation="MEITSLGHAGLRLETTTGLRLLMDPWLSRRGAFLGAWHQLPSND
                     HLDTPELLEVDWVTVSHEHLDHMDLDLLRRLPPSARVLINRYPSRNFHQRLVEAGVRG
                     VVEVAPWQRFPLNTKGDWLLFVPEQSPMCHDTGVLVHAGGVTLLHCNDARLTVAQCRR
                     AAVEAGGTIDVMAVQMSGASWHPIRYDYPPEILARVSGEKRTGKFKAVTRLLRGVRPR
                     LAVPFAGPPCFLDPALRDNNRFIPEPGIFPDLDTATSWLAEHLPAQASMRWLPGDRYD
                     VPSGHYTADPRWDGFDFSFEQLEQWLDGYASDRKADLEAVYADHPEPDEGLGERFADH
                     FARLGELNAYFLERIAMVVRFEVEGRGGGRWDVHLGPDKVRVDLAGRSRDSDVNYRFQ
                     VGSRWLAPVIDGAIGWEDLLLSLRFTASRSPDVYNDYFVGLLKHADGEALEAVERYET
                     SRDHSETITVDTPGGQYEIGRWCPHASEDLAVGAVLDGAVLRCLGHNFDFDVTTGECL
                     NARCDPLPTRRVTEAEPQQRALRQAT"
     gene            8019..>8840
                     /locus_tag="VF995_10960"
     CDS             8019..>8840
                     /locus_tag="VF995_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344119.1"
                     /translation="MTSGILVNAGERLGIDCAVDWAGALISEAADGGLLDGDPSAGTV
                     LIRVEASRDPFDTAGWEPIARGASGNHGSLVLRNLLSTGFDLRVDTGAGPGPGPGTAG
                     TAAVPPRFTFRWRPGRRERAMALALRSRFHLLARSALLRYPALWAAGTRGRAPLHASA
                     CTAGNNGVALLAGPGGVGKSTLVAKELAAGGSAICDNVCVSDGAGAWGVVEPLRVEGG
                     SSAGGGRRMQHGRREAHMPGRVPSLVPDRIVVLRRGVTGTASVRPCSPEAATRVLV"
BASE COUNT         1332 a         3170 c         3122 g         1216 t
ORIGIN      
        1 gcccaggttc tggctggagg gcgtgggcgg ccccgccggg cggcggtcag cgctgttccc
       61 gccgcgacga ccccggcggc cagcagcggc agcgccgcgg tcacataagt gccgtggccc
      121 gcgcgtcgct cggccgcgac ctcgccgagc agatcacgga acgaacgccg ctccagccag
      181 ctgtgcagat gctgccggac ccccccgtcc acccggtcgg gcgccggacc ccgcccgacc
      241 gccaccccga tcgccaccac ggcgaccagg caggcgatcc cactccagac tcgcacgcgc
      301 acgctcaccc gcagcccacc ctgatcctga cctccacatc gacctttccg agggaatgag
      361 atgacgatcc gccgccggtc acatcgccag caactccggc tcccggtccg gctccggacg
      421 gccgcggtcg cggcgctggt ggtcgcgctg acagccggct gcggcagcgg cagcgggcgc
      481 cgtcagcccg cggcccagat ggtgacgcca cccaagccag cgccgcggag cgggccgccg
      541 ccggtcctcg ccgtgcactt cgacaggggg aagtacccgc tgctcggcag ccgcgaggca
      601 gacctgtcca aggccgccga ggcgttcaac gcggtgctgg tctcctacgg cgaccagcag
      661 ctggtggagc aggcacgggc tcgcggcctc aaggtctacc tcggcttcga cgagcaccag
      721 gagttcgccg gcggcaagga catcagcgcc gacgtgcggg acctggtgcg gttcgccagc
      781 gccaaccgcg accagatcgc cgggatccgc gtcgccgacc gggtcaacga gggcctcacc
      841 ccggcgcaga cgctcggcta cctgcgggcc accggcgggg tgctgcacca gcggctccct
      901 gacgtcccgg tgatcgtcga cgtgtcggac tggcagctca cctgcgacct gctcggccag
      961 aacccctgcg gcaaggcccg ctcggccgcc taccggtatc agaccaacgc ggggttgctg
     1021 cagatctacc acagcggcta tgtcgacggc ttcatgctcg ccgacgggct ggcccgcgac
     1081 gacaccgact gcggcggctg caccgcccag gcctccgacg cgactgccca gaccgccgcg
     1141 tggaagcggg cccgtgccct gttcccgcat ccgttcctgc tgttcgcccg ggtcgcgccg
     1201 ctgtcgttcc cccaggcgca ctacccggcc gacgccgtcc aggcggccct ggcggtcagc
     1261 gtctacgaga cgatcccgct gcagcagggc gccgacggcg tcgacctgtg ggcgtggagc
     1321 cgccacttcg acggcacggt gcgaaccttc ctcgacaagg acggcgccag caacccgctg
     1381 tggcagcagt tcgtgacgct cgccacggcg atgcacgccc aggcgcgccc ttcctgatcg
     1441 cgctttcctg gtcgtgatgg tcttcgcacc gggaggtcag acgcggctga gcgtccggct
     1501 gaagatccgc gccggctcgg tgaacgccac cggcggccgg gccagggtga cggtgccgag
     1561 tggctgcgtc cgccgggtgc ggccaccggt gacgatgtag agcagcgtct cgcccatcac
     1621 gccgcaggtc cgccgcagcg tcggcgcggc caggaagtca aggtcgacgg tgaccttggc
     1681 cggcacgacc acctgcaggt agtactcctc caggtcctcg tcgaccgagc gcagctcgtc
     1741 ccggtcgcgc agccgcaccg cggcgggccc ggtcaggccc ggccggtagc ggaacacgtc
     1801 ctcgcagccg ggcaggtagc ggctggcgag gtcgggggtc tcggggcggg ggccgaccag
     1861 ggtcatctcg ccccggagga tgttgagcag ctgcggcagc tcgtcgaggc cggccaggcg
     1921 cagcagcttg ccgagggggg tgagccgcag gtcgtcggcg gtggtgacgt tcggcccgga
     1981 ggcgccggcc cgcatggtgc ggaacttcag cagcgtgaac gggacgccgc cctgcccgac
     2041 acggctctgg cggaacagca ccgctccggg gctgctcagc ctgaccagca gcgcgacgac
     2101 cccgaacacg ggcgacagca ccaccagcgc gaggccggcg accagcaggt cgagcgcccg
     2161 ccgggggacc cccggcggga tgccgagccg cccggacagg ctcgacctgg acctggagat
     2221 gaacgtggac atgaacctgg tcctggacct gggccctgag gtcgccgctt gactcatcga
     2281 cttctccgtt ccggaccgcc ggacgtcgcc gccgtcctcg ctctcactcg cgcacgtgct
     2341 gctgctgggg ccgggtgggg gaggacggtg ggaggcgggg aggttgcggc cgggccggga
     2401 atgccgagca ggcggcagta ggtctggtcg atcagggcgc acatcagcgc cgggtcgaac
     2461 atccagcgga cccagcggct gccggcgtcg cccatgcggc tcgcctcgcc aggatggtcg
     2521 aggagccaca gtaccccggc tgccagcgac gccgggtcgc cgggggtggc gagcagcccg
     2581 gtcgcgccgg ggacgatcag gtcgggcaca ccgttgacag cggtggcgac caccgggcgg
     2641 ccggaggcga ccgcctcggt caacgcccgc ccgagcccct cgtacagcga ggagatcacg
     2701 aacacgtcga aggcgctggc gatccggggc gcgtcggcgc ggaacccggt gaagcggacc
     2761 tcgacgccga gccgcgccgc ctcggccttg gcgtcgtcaa gcagtgggcc ctcgccgacc
     2821 atgacgaagc aggcgtccgg ccgggatgcg gcgaccgtgg cggccatgcg gacgaagtcc
     2881 agcggcgcct tctggtactc cagccgcccg accgtgccga tcagcggcgt gtgcggcgcg
     2941 atcccgaatt cggcccgggc ggcggggtcc ggcatggtgg ggatctggtc aagctcgacc
     3001 gccgatggca ccaccgtgac gctgccaggc ggggcgatgc gctgctcgac cgcctcgcgg
     3061 gcgacccggg gcgcgaccga caggaactcg tcggtgaacg gccgcaccag ccggtccagc
     3121 agcaggtaga cgcgtttgcg ccgcttgctc atgaagtcgt ggaacggcag gccgtggaag
     3181 gtgtgcagga tcaccggggt atggcacagc caggcggcca gccggccgag gaagcccgcc
     3241 ttggccgagt gggtgtgcac gatcgccggg cgctcccgcc ggatcaggcg gacgagctgg
     3301 acgagcacgg agaggtcgtt gaagggggag atggtctcgc ggaagcgctt gagccggacg
     3361 gtcctgatgc cggcctgctc ggcccgctcc cacagcgggc cgccgtcgca gccagccacc
     3421 caggtctcat agcgtcccgg gtcggcgccg agcacggtca gcagggtgtt gccgccggcg
     3481 ccggcccaga acttggtgat cacgtgcagc accttgacct gggagggcag cggctgcggc
     3541 ggcagccccc cggcggcgtc agtgacgacc tgcccgccgc tggccaggtc gctcggccag
     3601 gcaggcacgc gggtcccggc ggggacccgg ttggcgttca tcgcagggcc gccttggcgg
     3661 ggtggcggcg gaggctggcc agcgtctcgc agacgcggtc gacgtcgtcc agcgccatcc
     3721 cctggaacag cggcagcgac acgatccgct cgaaggcggc gtcggcgccc gggtgggtgc
     3781 cgagctgctc ggggtcgagc agctgctgga aataggggaa gtggtgcacc gggatgaagt
     3841 ggaccgaggt gccgacacct cgctcggcga gggcggcgat cagctcgtcg cggctcaggc
     3901 cgtactcggc ttcgacctgg acaacgtaga tgtgccacgc atggcggccc cactccggcc
     3961 gcgccggcag tcccaggccc ccgacggcgg cgaggccgtc gtcgtagcgg cgggcgagca
     4021 gcgcgcgacg ctcctgccag gcggccaggt ggcgcagctg cgcgcggccg atggcggcct
     4081 gcaagtcggt catgttcgcc ttcagcccgg ccacctcgac gctgtagcgc cacgcctcgc
     4141 cgggcaggta acgcttccac gcgtccttgc tcatgccatg caggcgggtg cggcgcaggt
     4201 ggtcagcgac ctcctggtcg gcggtggtga ccatgccgcc ctcgccgatc ggcaggttct
     4261 tggtggcgta gaagctgaaa caggtagcgg cggagcagct ccccaccggc cgcgcgccat
     4321 gccaggtgcc cagggcatgg gcggcatcct cgacgacccg cccaagcggc aggcccacgg
     4381 cctcggccag ctcctccacc ggggagggca gcccggcgta gtgggtgatc accatcgcgt
     4441 cgacgccgcc ggcccgccgc gccgccgccg cgcaggcagc cgcgtccgag gagagcgtca
     4501 ccgggtcgac gtcggcgagc accggcacca gcccggcgtg gacgatcgca tggaccgccc
     4561 cgcagaaggt catcgtcgag gtgagcacct tggcgccgcg cggcaggcgc agcgctcgca
     4621 gggccagctc gatcgccgcg gtgcaggagg cgaccgcgac cgcgtggtcg gcgtgcaccc
     4681 actccgcgaa ctcgcgctcg aaggcggcca cctccgggcc ggtcgtcagc cagccggatc
     4741 gcagcacctg cgcggccgcc tcatgggcct ccgcgcagac ctccgcgcgc gcgaacggaa
     4801 tctcgaactc aatggccaca ggtcccccga aatttccccg aatgcctcga aattttcctc
     4861 atcccgtggt gacggccgac ggcaccggag ccgttccctg gctgttgccg tggctgttgg
     4921 catgaccgtt gccgttgcca ttcccgatgc cgaactccag cggcagccgg ccaaaggacc
     4981 catagagatg cgcgtactcc gcgatcccgt cctcgagcgt gtgggccaag ccgaaaccaa
     5041 gggcgcggag ccgactccca tcgacctcat aggagatctg gttgaggatc tccttgtcgg
     5101 catgggtgat ttgaaccttt gggaaatggc tccggaggag cgcgacgatc tgattcacgc
     5161 tcgcgttctc gccgaccacg ttgaagatgc cgtcatgggt ttggtcgccg gcaaggcaga
     5221 aggcgatcgc ccggctcacg tcggtgacgt gaatgaaggg ccggagctga ttgccggcgc
     5281 cgtagatctc aatgggggcg ccgacggccg ctcgaaaagc gaagatgttg accacggtgt
     5341 ggacgcgcaa cccgggagag tatccataga cggtggcgaa tcgaagcacc gtcgggtgca
     5401 accgcccacc cgtgtcggtc gacaggccga ggacgtcctg ctcgcccttg agcttgaact
     5461 cggcgtacgg gctggcgggg aagcaggggc tctcctcgtc gacgattccc gaggtcgggc
     5521 cgtacacgct ggtggacgag gcatagatga aacgcttgac gctcggcgcc atgcgcgccg
     5581 cccgcaccac gttcaccgtc ccgccgtaat tgatctcctc agtcgcatcg cggcgatcga
     5641 aggaaatcgt tgcgttgcag agcgcggcga gatggaccac cacgtcggtg tcgtcgccga
     5701 gggcggcctg aacgtcgccc tcgttacgga tgtcgccaag gacgaattca tagctgacac
     5761 cgtcgggaag gttcatcagt gatgggaact tctcttcgcg catgttgtcc aatacgcgaa
     5821 cacgtcgaac agggagtgat ccatccgacg ccaacatgtg caagagccta gagccgatgt
     5881 agccggctcc ccccgtgaca aggatgttca cacgccctcc ccgaaccgga tataaggtcg
     5941 tactggagtc cgcaaggtga cgctcaggtg aatctgcggc gacctgttga ctatgcgtcg
     6001 cttcgaagcg gcagcaaccg gcaagtcgaa tgaaacgtgc ctacgctatt tgcctggcag
     6061 gtctcatgag cggtgctacg caagatgcgg gtggtgtacg ccattcagcg cgattaatga
     6121 ttccgccatg gctgatgaag aactgacgac gtcgagccca cggccagtcg acacgccgct
     6181 cgacgcggag gcgcaggcgg ctggagaacc tctcgccgac gactacagcg acgcggagcg
     6241 cgacgaggtg gccgcacgcc tcgcctcgct cggctatatc gacgaatagc gccgccggcc
     6301 acgggcaaac ggacatggag atcacctcgc tcgggcatgc cgggctgcgg ttggagacga
     6361 ccaccggcct gcgcctgctc atggacccct ggctgtcgcg gcgcggcgcc ttcctcggcg
     6421 cctggcacca gctccccagc aacgaccacc tcgacacccc ggagctgctc gaggtcgact
     6481 gggtcacggt gtcccacgag cacctcgacc acatggacct ggacctgttg cggcgcctgc
     6541 cgccgagcgc ccgggtgctg atcaaccgct acccgtcccg gaacttccac cagcgcctgg
     6601 tggaggccgg cgtccgcggc gtcgtcgagg tcgccccctg gcagcggttc ccgctcaaca
     6661 ccaagggcga ctggctgctg ttcgtccccg agcagtcacc catgtgccat gacaccggcg
     6721 tgctggtcca cgccggtggc gtcacgctgc tgcactgcaa cgacgcccgc ctgacggtgg
     6781 cccagtgccg ccgcgccgcc gtcgaggccg gcggcaccat cgacgtgatg gcggtgcaga
     6841 tgtcgggcgc gagctggcac cccatccgct acgactaccc cccggagatc ctcgcgcgag
     6901 tctccgggga gaagcgcacc ggcaagttca aggccgtgac gcgcctgctg cgcggggtgc
     6961 ggccgcggct cgccgtcccg ttcgccgggc cgccctgctt cctggacccg gcgctccgcg
     7021 acaacaaccg cttcatcccc gagcccggca tcttcccgga tctcgatacc gccaccagct
     7081 ggctggccga gcatctgccc gcgcaggcgt cgatgcgctg gctcccgggt gaccgctacg
     7141 acgtcccaag cggccactac accgccgacc ctcggtggga cgggttcgac ttctcgttcg
     7201 agcagctcga acaatggctc gacggctacg ccagcgaccg caaggccgac ctcgaggcgg
     7261 tctacgccga ccacccggag cccgacgagg gcctcggcga gcgtttcgcc gaccatttcg
     7321 cccggctcgg cgagctgaac gcctacttcc tggagcggat cgccatggtg gtccggttcg
     7381 aggtggaggg gcgtggggga ggacggtggg acgtgcacct cggccctgac aaggtgcggg
     7441 tcgacctcgc cggccgcagc cgcgacagcg acgtcaacta ccgcttccag gtcgggtcgc
     7501 gctggctcgc gccggtgatc gacggggcga tcggctggga ggacctgctg ctgtcgctgc
     7561 gcttcaccgc gtcacgctcg cccgacgtct acaacgacta cttcgtcggc ctgctcaagc
     7621 atgccgacgg cgaggcgctg gaggcggtgg agcgctacga gaccagccgc gaccactccg
     7681 agaccatcac cgtggacacc cccggcgggc agtacgagat cggccgctgg tgcccacacg
     7741 ccagcgagga cctcgcggtc ggcgcggtgc tggacggcgc ggtgctgcgc tgcctggggc
     7801 acaacttcga cttcgacgtg accacgggcg agtgcctcaa cgcccgctgc gatccgctgc
     7861 caacccggcg agtcacggag gcagaaccgc agcagcgggc gctcaggcag gcgacgtagc
     7921 cgaactcact ctttcacctg atagtggtcg gttctcgctg acatagcgta agtgaacaag
     7981 gagtagcaga tgcgcatcgg gtggaaggcc gggggtcgat gaccagcggg atcctcgtca
     8041 atgccggtga acgtctcggc atcgactgcg ccgtcgactg ggcaggcgcg ctgatcagcg
     8101 aggccgccga cggcgggctg ctcgacggag accccagcgc cggcacggtt ctgatccggg
     8161 tggaggccag ccgcgacccc ttcgacaccg ccggctggga gcccatcgcc cgtggcgcct
     8221 ccggcaacca cggatcgctg gtgctgcgca acctcctctc caccggcttc gatctccgcg
     8281 tcgacaccgg cgccggccct ggccctggtc ccggcaccgc cgggaccgcc gccgttccgc
     8341 cccggttcac cttccgctgg cgccccggac gccgcgagcg ggccatggcg ctggcactcc
     8401 gctcgcgctt ccacctgctg gcacggtcgg cgctgctgcg ctacccggcg ctgtgggcgg
     8461 cgggcacccg cggccgtgcg cccctgcacg cctcggcctg caccgccggc aacaacggcg
     8521 tggcgctgct ggccgggccc ggcggggtcg gcaagtcgac cctggtcgcc aaggaactgg
     8581 ccgccggcgg cagcgccatc tgcgacaacg tctgtgtgag cgacggcgcc ggcgcctggg
     8641 gcgtagtcga gcccctgcgc gtcgagggcg gcagctcggc cggcggcggc aggcgcatgc
     8701 agcacggccg ccgcgaggcc cacatgcccg gccgggtccc gagcctcgtc cccgaccgga
     8761 tcgtggtgct gcggcgtggt gtcaccggca ccgcctcggt acggccctgc agtcccgagg
     8821 ccgccacccg ggtcctggtc
//
LOCUS       DASWDC010000343         6186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_229364, whole genome shotgun sequence.
ACCESSION   DASWDC010000343 DASWDC010000000
VERSION     DASWDC010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229364"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VF995_10965"
     CDS             complement(<1..649)
                     /locus_tag="VF995_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344120.1"
                     /translation="MADSDTNAAAARRMAAPGPMAVDWEERVDFRRLHTYRLARARQA
                     LDASELGAVLAFDMNNVRYLTSTHIGEWARDKLITFTLLARGAEPVIWDFGSAAKHHR
                     LYCPWLDPANSRAGLTGLRGAVTPEAGLFERAAREVKAELNRAGVAGMPLGVDVSELP
                     LLVELHRLGVEVRDGQQVMLAARQALDASELGAVLAFDMNNVRYLTSTHIGEWARD"
     gene            complement(690..1574)
                     /locus_tag="VF995_10970"
     CDS             complement(690..1574)
                     /locus_tag="VF995_10970"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9344121.1"
                     /translation="MKVGFIGLGVMGSRIVKRLLDAGHEVGGYNRTKAKAEWLLSHGL
                     TIRDSPREVAEAAEFTLSMVSDTSALRAVTGGPDGAIAGLGDGQVYVDMSTVSPALSR
                     ELADTVTQVGASMLDAPVSGSVITLERGELTIMVGGEAATFERAHPLLTDIGPRVIHV
                     GANGQAALMKIAINLSLPIQLAALYEGLLIAEKGGIPREVALRTMLGSVIASPAMKYR
                     GPFVEELPGEAWFDVNMMQKDLQLALEMGRRLDVPMPATATTNELLTAARAFGFEKED
                     FAVLYHTLARMSGLEGSP"
     gene            complement(1801..2922)
                     /locus_tag="VF995_10975"
     CDS             complement(1801..2922)
                     /locus_tag="VF995_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9344122.1"
                     /translation="MSDRPPRLRDVARAAGVHVATASRALNDETASMLSAATVERVRQ
                     AAEALGYRVNGMARALKTRRSLAIGMLVPDITNPFFPPVVRGAEDSLAKAGYSLVLAN
                     TDNDDQRARREVRIMLEGRVDGLLLAMARRRDPLVAELSAGPVPVVLVNRTVDHVGAF
                     AVVPDDHAGAVLAVEHLYELGHRRIAHVAGPQTTSTGARRLAGFTQAAHSLGVAMAWI
                     EAAQFNELEGARAASTLLRGAAGDHPSGGLPLGRPERPAAVGGASLVRPPSPQPRPTA
                     IVAANDLLALGVLDVAARQGLACPADLSVVGFNDMPFVDRLQPPLTTLRVAEYDLGYR
                     AAALLLERLDRPERRAETLLLTPELVVRGSTAPPPATIS"
     gene            complement(3100..5505)
                     /locus_tag="VF995_10980"
     CDS             complement(3100..5505)
                     /locus_tag="VF995_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9344123.1"
                     /translation="MRDGSSGHQHRVLGKPLRRVEDERLLRGVGPYVADLDLEGAAWV
                     TYVRSSEPHARLVAVRTEAAASAPGVLAVVTAADVDLAPLAPAPPVLEQRMARPWLAD
                     GVVRFVGEPVAAIVSETREQGVDAAELVEVDYDWLDPVPDVEAADRGEVLLFPEVGSN
                     LAFAVGVGEGEDQEPNAALFDGCEVVVRQRQVIQRLAPCPLEGRAAAAVWGASGDGDA
                     GAAGRAGADGEGDRAGALSTLTFWISTQRPHEVRDLLAGALGMAPSRVRVRVPEVGGG
                     FGAKGNPYPEELLLAWLARRVGRPLRWVETRSESMLGLSHGRAQVQYLELGGRRDGTL
                     LAYRVRVLQDAGAYLRFSAFLPARVRQLACGNYTIPRVEAGGRSLVTSTVPVGPYRGA
                     GRPEATMAIERAIDLFAAEAGLDPVEVRRRNLVSADAFPWTTATGAVYDSGDYQRALD
                     LVLEAGGYQAARAEQARRRERGGPLRLGIGVALYVEVAGGGEHLSEYGAVEVTRDGAA
                     VVRTGSCATGQGHETTWAMIASEQLGIPVERITVLRGDTAEVARGAGTIGSRSTQSGG
                     VAVHRAAVALRERAVELAAGLLEASPEDVVLDRDAGRFHLAGAPTVSRTWAELAAASD
                     RGGSNSRLAAEVDLDDGALTYPSGAYLAVVEVDTDTGQVRLRRLVAVDDAGNILNPLL
                     AEGQLHGSIAQGVAQALYEEFRYDDDANPLSGTLQVYGPPTAADLPAFDLVELETPSP
                     RNDLGVKGLGEAGCIGAPPAVLGAVVDALAPLGVRHLDMPATPERVWQAIRAARRAGP
                     PWA"
     gene            5669..>6186
                     /locus_tag="VF995_10985"
     CDS             5669..>6186
                     /locus_tag="VF995_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344124.1"
                     /translation="MAVTARVEHVGSLLRPSYLLEAREARDSGRLDPAAFKAVEDRAV
                     KEVLALQREVGCPVVTDGELRRDSFQSELAAAVDGFAGVDMDAWLWGEWHAGAEGPGD
                     LTVARPAALGVVAPLRARRRLAAEEFTFVRGVVGDDPHRVAKVTLPSPSLFANLWSPA
                     EHASGRSDAVHG"
BASE COUNT          795 a         2297 c         2284 g          810 t
ORIGIN      
        1 tgtcgcgcgc ccactcgccg atgtgggtcg aggtcaggta acggacgttg ttcatgtcga
       61 aggccagcac cgcgcccagc tcgctcgcgt ccagcgcctg gcgggcggcg agcatgacct
      121 gctggccgtc gcgcacctcg acgccgaggc ggtgcagctc gaccagcagg ggcagctcgc
      181 tgacatccac gccgagcggc atgccggcga ctccggcgcg gttcagctcg gccttgacct
      241 cgcgggcggc gcgctcgaac agcccggcct cgggggtgac cgcgccgcgg agcccggtga
      301 gcccggcgcg ggagttggcc gggtcgagcc aggggcagta gaggcggtgg tgcttggcgg
      361 cggagccgaa gtcccagatg accggctcgg cgccgcgggc cagcagggtg aaggtgatca
      421 gcttgtcgcg cgcccactcg ccgatgtggg tcgaggtcag gtaacggacg ttgttcatgt
      481 cgaaggccag caccgcgccc agctcgctcg cgtccagcgc ctggcgggcg cgagcgaggc
      541 gataggtgtg gaggcggcgg aagtcgaccc gctcctccca gtcgaccgcc atcgggccgg
      601 gcgcggccat ccgccgcgcc gccgctgcgt tggtgtcgct gtccgccacg gccggaccct
      661 cccttcgctg gtgcctgcct gctcgctccc tacggtgacc cttcgaggcc ggacatgcgc
      721 gccagggtgt ggtagaggac cgcgaagtcc tccttctcga agccgaaggc gcgggctgcg
      781 gtgagcagct cgttggtggt cgccgtggcc ggcatcggca cgtcgagcct gcggcccatc
      841 tccagtgcca gctgcaggtc cttctgcatc atgttgacgt cgaaccaggc ctcgccgggc
      901 agttcctcga cgaacgggcc ccggtacttc atcgctggcg aggcgatcac gctgccgagc
      961 atggtccgca gcgccacctc gcgggggatg ccgcccttct cggcgatcag cagcccctca
     1021 tagagtgcgg cgagctggat cgggaggctc aggttgatgg cgatcttcat gagggcggcc
     1081 tgaccgttgg cgccgacgtg gatgaccctg ggtccgatgt cggtcagcag cgggtgggcc
     1141 cgctcgaagg tggcggcctc gccgccgacc atgatggtca gctcgccacg ctccagcgtg
     1201 atgacgctgc cggagacggg cgcgtcgagc atgctggcgc cgacctgggt gaccgtgtcg
     1261 gccagctcgc ggctgagggc ggggctgacg gtggacatgt ccacgtagac ctggccgtcc
     1321 ccgagcccgg cgatggcgcc gtcagggccg ccggtcaccg cgcggagcgc gctggtgtcg
     1381 gacaccatcg agagggtgaa ctccgccgcc tcggcgacct cgcgcggcga gtccctgatg
     1441 gtgaggccat gggagagcag ccactcggcc ttggccttgg tgcggttgta gccaccgacc
     1501 tcatggccgg cgtcgagcag gcgcttgacg atccggctcc ccatgacgcc gagcccgatg
     1561 aatccgacct tcatggggac caccgccgac gaagacgacc gcttggacga ccgcttggac
     1621 gaccgcctgc cgccgggttg cgatgatccc gccgggcggc gacgtccggc ctggtcggcg
     1681 gcaaccggtc gatggttcta tacggtggtc ccatgccctc ccccgggtct ggttcctgcg
     1741 ccgcgttgat tcgttccctt tacaatcgtt tgctgacgct aaccctgccc cttaccagtg
     1801 tcaactaatg gtcgccggcg gcggcgccgt agagccgcgg acgaccagct cgggggtgag
     1861 cagcagcgtc tccgcgcggc gctcgggccg gtcgagccgt tccagcagca gcgcggcggc
     1921 gcggtagccg aggtcgtact cggccacccg cagcgtggtc agcggcggct gcagccggtc
     1981 gacgaagggc atgtcgttga agccgaccac cgacaggtcc gccgggcagg cgaggccctg
     2041 ccgcgccgcc acgtcgagga ccccgagggc gagcaggtcg ttggcggcga cgatcgcggt
     2101 cgggcgcggc tgggggctcg gagggcgcac gagcgaggcc ccgcccacag cagccggtcg
     2161 ctctggacgg ccgagcggca gccccccgct gggatgatcg ccggcggcgc cgcggagcag
     2221 cgtgctggcg gcgcgggcgc cctccagctc gttgaactgc gccgcctcga tccaggccat
     2281 cgcgacgccg agcgaatggg cggcctgggt gaagcccgcc aggcgccgcg cgccggtcga
     2341 ggtggtctgc ggcccggcga cgtgggcgat gcggcggtgg cccagctcgt agaggtgctc
     2401 gacggccagc accgcgccgg cgtggtcgtc ggggacgacc gcgaacgcgc cgacgtggtc
     2461 gacggtgcgg ttgaccagca ccaccgggac cggcccggcg gacagctcag cgaccagcgg
     2521 atcacggcgg cgggccatgg ccagcagcag gccgtcgacc cggccctcca gcatgatccg
     2581 cacctcgcgg cgggcgcgct ggtcgtcgtt gtcggtgttg gcgagcacca gcgagtagcc
     2641 ggccttggcg agcgagtcct ccgcgccgcg gaccactggc gggaagaacg ggttggtgat
     2701 gtcggggacc agcatcccga tcgccagcga gcggcgggtc ttgagcgcgc gggccatccc
     2761 gttgacccgg tagccgagcg cctcggcggc ctgccggacc cgctcgaccg tggcggcgct
     2821 cagcatcgag gcggtctcgt cgttcagcgc ccgcgaggcg gtggccacgt ggacccccgc
     2881 cgcccgggcg acatcgcgca gtctcggcgg ccggtcgctc atctaccgct cgcgctcact
     2941 tcgcactcgt tgaaaccgtc cgaaccccga caagttccca tacggctgac gacaaacgat
     3001 tgcaaactgt gcggcgcccg tcaatcggcg ctcccccgcc gctcgcacac cagcggcacc
     3061 gaggccaccg agggcgccag gggcaccggg cggagcgccc tacgcccatg gtggaccggc
     3121 tcgccgggcg gcccggatcg cctgccagac tcgctcgggg gtcgccggca tgtcgaggtg
     3181 gcggacgccg agcggcgcca gcgcgtcgac caccgcgccc agcacggccg ggggcgcgcc
     3241 gatgcagccg gcctcgccga ggcccttgac gccgaggtcg ttgcgcgggc tcggcgtctc
     3301 cagctccacc aggtcgaagg cgggcaggtc cgcggcggtg ggcgggccgt agacctgcag
     3361 ggtgccgctc agcgggttgg cgtcgtcgtc gtagcggaac tcctcgtaga gcgcctgggc
     3421 gacgccctgg gcgatgctgc cgtgcagctg cccctcggcc agcagcgggt tcaggatgtt
     3481 gccggcgtcg tccaccgcga ccagccgccg cagccgcacc tgcccggtgt cggtgtccac
     3541 ctcgaccacg gcgaggtagg cgccagacgg gtaggtcagg gctccgtcgt ccaggtcgac
     3601 ctcagcggcg agccggctgt tgctgccgcc gcggtcgctg gcggctgcca gctccgccca
     3661 ggtgcggctc acggtcggcg cgccggccag gtggaaccgg ccggcgtcgc ggtcgagcac
     3721 cacatcctcg gggctcgcct ccagcaggcc ggcggccagc tccacggcgc gctcacgcag
     3781 cgccaccgcc gcgcggtgga cggcgacgcc gccgctctgg gtggagcggg agccgatcgt
     3841 cccggctccg cgcgccacct cggcggtgtc gccgcggagc acggtgatcc gctccaccgg
     3901 gatgccgagc tgctcgctgg cgatcatcgc ccaggtggtc tcgtggccct gcccggtggc
     3961 gcaggagccg gtgcgcacca ccgcggcgcc gtcgcgggtc acctccaccg cgccgtactc
     4021 gctgagatgc tccccgccgc cggccacctc gacgtacagc gccacgccga tgccgagccg
     4081 cagcggcccg ccgcgctcgc ggcggcgtgc ctgctcggcg cgcgccgcct ggtagcctcc
     4141 cgcctccagg accaggtcga gcgcccgctg gtagtcgccg ctgtcgtaga ccgcgccggt
     4201 cgcggtggtc caggggaagg cgtcggcgga gaccaggttg cggcgccgca cttcaacagg
     4261 gtccaggccg gcctcggcgg cgaacaggtc gatcgcccgc tcgatcgcca tggtcgcctc
     4321 cggccgcccg gccccgcggt aggggccgac cggcacggtg ctggtcacca gcgagcgccc
     4381 tccggcctcg accctgggga tcgtgtagtt gccgcaggcg agctgccgga ccctggccgg
     4441 caggaaggcg gagaaccgca ggtaggcccc ggcgtcctgc agcacccgca cgcggtaggc
     4501 gagcagcgtc ccgtcgcggc ggccccccag ctccaggtac tgcacctggg cccggccgtg
     4561 cgacaggccg agcatgctct ccgagcgggt ctccacccac cgcagcggcc gcccgacccg
     4621 ccgcgcgagc cacgccagca gcagctcctc tgggtagggg ttgcctttgg cgccgaaccc
     4681 gccgcccacc tcggggacgc ggacccgcac ccgcgacggc gccatcccga gcgcccccgc
     4741 cagcaggtcc cgcacctcgt gcggccgctg cgtcgaaatc cagaaggtca gcgtggagag
     4801 ggcgcccgcc cggtccccct ccccgtcggc gcccgcacgc ccggcagcgc ccgcgtcgcc
     4861 gtcgccactg gcgccccaga ccgccgcggc ggcgcggccc tccagcgggc agggcgccag
     4921 ccgctggatc acctgtcgct ggcgcaccac cacctcgcag ccgtcgaaga gggcggcgtt
     4981 cggctcctga tcttcgccct cgcccacgcc cacggcgaag gcgaggttgc tgccgacctc
     5041 ggggaacagc agcacctcgc cgcggtcggc ggcctccacg tcgggcaccg ggtcgagcca
     5101 gtcgtagtcg acctcgacca gctcggcggc gtccacgccc tgctcccggg tctcgctgac
     5161 gatcgcggcc accggctcgc ccacgaagcg gaccaccccg tccgccagcc aggggcgcgc
     5221 catccgctgc tccagcaccg gcggcgccgg cgccagcggc gcgaggtcca cgtcggcggc
     5281 ggtcaccacg gcgaggaccc cgggcgcgga cgccgccgcc tcggtcctga ccgcgaccag
     5341 ccgcgcgtgc ggctcgcttg agcgcacata ggtcacccac gcggccccct ccaggtcgag
     5401 gtcggcgacg tatggcccga cgccgcgcag cagccgctcg tcctcgaccc gccgcagcgg
     5461 cttgccgagc acgcgatgct ggtggccgga cgacccatca cgcatgcgaa caatctacgc
     5521 gatctcgcgt cgccgcatgc gaacgattgc cagcagccgc cagcacgcac ggcgtggcga
     5581 gcacggacct tcctcagcca gctcgatgcc tgcccgatcg cgtcgggagg cacagcgcca
     5641 agccgccccg gtccgatacc gtaaggccat ggctgtcacc gcgcgggtcg agcacgtcgg
     5701 cagcctcctg cgcccgtcct acctgctgga ggcgcgggag gcgcgtgaca gcgggcggct
     5761 ggacccggcg gccttcaagg cggttgagga ccgggcggtc aaggaggtgc tcgccctcca
     5821 gcgggaagtc gggtgcccgg tggtcaccga cggggagctg cggcgggact cgttccagag
     5881 cgagctggcc gcggcagtcg acgggttcgc gggtgtcgac atggacgcct ggctgtgggg
     5941 cgagtggcac gccggcgcgg aagggcccgg cgacctgacg gtggcgcggc ccgccgcgct
     6001 cggggtggtc gcgccgttgc gggcgcggcg ccgcctcgcg gccgaggagt tcacgttcgt
     6061 ccgtggcgtc gtgggcgacg acccgcaccg ggtcgccaaa gtgaccctgc cgagcccgag
     6121 cctgttcgcc aatctgtggt cgccggccga gcatgcaagc gggcggagcg atgccgtcca
     6181 tgggag
//
LOCUS       DASWDC010000344         3586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_996970, whole genome shotgun sequence.
ACCESSION   DASWDC010000344 DASWDC010000000
VERSION     DASWDC010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996970"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VF995_10990"
     CDS             complement(<1..279)
                     /locus_tag="VF995_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344125.1"
                     /translation="MASSPAPVLGAAERRRLAPLAAALVRARQPGPPRLEPVVGALVP
                     GGGHGGALRQPPAALFAGSFNPLTRAHAALAVAGHRAGCDPVVLTMAPV"
     gene            complement(335..1366)
                     /locus_tag="VF995_10995"
     CDS             complement(335..1366)
                     /locus_tag="VF995_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9344126.1"
                     /translation="MLQSKPEAAVSSISSTDGRRNGAGTGLDLPSDDEALDAYSMVVS
                     TVAERVLPSVASLRVSRRVPGGWQAQGAGSAVVLTPDGFLATSAHVVDGSTRGRAILA
                     DGRELPFEVVGRDPLSDLAVVRASGADLEPVSLGNADRLRVGQLVVAIGNPMGLRGSV
                     TAGVVSALGRSFPTSDGGASRMVENVIQTDAALNPGNSGGALVDGRARVVGVNTAVAG
                     VGLGLAVPINVTTRRIIAALMQEGRFRRAFFGVAGGRRPLPPRAARALDRDNGVEVVE
                     VVPGSPADRAGLRREDLLVDVDGTPVADAGALQRLMVTEAIGRPLRVRVFRGGELLEL
                     TVVPVELRS"
     gene            1453..2607
                     /locus_tag="VF995_11000"
     CDS             1453..2607
                     /locus_tag="VF995_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9344127.1"
                     /translation="MPADAELLDAVHLAVQTSPRNLTLRLHLGYLLLEAGRPVEALEQ
                     CALVLVRVPDHGQALELATRAAADDARRALPALPRPPRATGFRVEPALLNFASVGGMT
                     SVKRQLAAIAEVSASPLLYGPPGCGKTFIARALAGELGAGFLAVKGSRMVERWDRDGR
                     HALSEAFALARASVPCLLFLDGLDALARSARDVTEQLLAELVGVGDQDGITVVAASER
                     PWEIPALLRGPGPAGRRSPAAGHGHGHGQGLGLGLGRGWGRGGVGPRLLVLPPDRVAR
                     QAIIRGALRGHPLDELNLAALAGRTEYFSATDLIALCDVAARVALQTAMTRGVRPIGM
                     DDFDRALRQLQPSPLAWFGLARDSLQHGGEDALDMDLRAYLRTVIPKGAA"
     gene            complement(2654..3055)
                     /locus_tag="VF995_11005"
     CDS             complement(2654..3055)
                     /locus_tag="VF995_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9344128.1"
                     /translation="MTIQRTHIASGSPFEPTIGFCRALRVGDRVLVSGTAPVWPDGSC
                     DPDPGVQARRCLEIILAALAEAGAGPEQVIRTRMYLTDPADWEAVGRAHGEIFGAVRP
                     AATMVVVAALLDPRWRVEIEAEAVADAGAPG"
     gene            3176..>3586
                     /locus_tag="VF995_11010"
     CDS             3176..>3586
                     /locus_tag="VF995_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344129.1"
                     /translation="MQPTRSFLESIGLPGGDAFDLPTSERRFPDGGQYRVEIPSVEGP
                     AALETVLAEGARRGVRIHRISQGSGIQLLTDEEIERMVALGGERDVEVCLFTGPRASF
                     DTGVQAATGSGRVVAGALRGADQLAYGVEDVLRAC"
BASE COUNT          458 a         1340 c         1333 g          455 t
ORIGIN      
        1 gaccggcgcc atcgtcagca ccaccgggtc gcagccggcg cggtgcccgg cgaccgcgag
       61 tgcggcgtgc gcccttgtca gcgggttgaa gctgccggcg aacagcgccg cgggcggttg
      121 gcgcagcgcg ccgccatgcc ctccacctgg aacgagggca cccacgaccg gctcaaggcg
      181 gggcgggccg ggctggcgag cgcggaccag cgcggcggcc agcggcgcga ggcgccgccg
      241 ttccgccgcg ccgagcacag gagcgggcga ggatgccatg ccctccagca tggcacgggg
      301 ttatgcggcg gtcgtccgcg tcggggtgtg gcggtcagct ccgcagctcg accgggacca
      361 ccgtcagttc gagcagctcg ccgccgcgga agacgcgcac ccgcaggggg cggccgatgg
      421 cttcggtgac catcagccgc tgcagcgcgc cggcgtcggc gaccggggtg ccgtccacgt
      481 cgaccagcag atcctcgcgg cgcaggccgg cacggtcggc ggggctgccg gggaccacct
      541 cgaccacctc gacgccgttg tcgcggtcga gtgcgcgggc ggcccgtggc ggcagcgggc
      601 ggcgcccgcc ggcgacgccg aaaaaggcgc ggcggaagcg gccctcctgc atcagcgcgg
      661 cgatgatccg ccgggtggtg acgttgatcg gcaccgccag gccgagcccg accccggcga
      721 ccgcggtgtt caccccgacc acccgggcgc ggccgtcgac cagcgccccg ccggagttgc
      781 ccgggttgag ggcggcgtcg gtctggatga cgttctcgac catgcggctg gcgcccccgt
      841 cgctggtggg gaaggagcgg ccaagcgcgc tcaccacccc ggcggtgacc gagccgcgca
      901 ggcccatcgg gttgccgatg gccaccacga gctggccgac ccggagccgg tcggcgttcc
      961 ccagggacac cggctccaga tcggcgccgc tcgcgcgcac caccgcgagg tccgagagcg
     1021 ggtcgcgccc gaccacctcg aagggcagct ccctcccgtc ggccaggatc gcccggcccc
     1081 gggtcgaacc gtccacgacg tgcgcggagg tggccaggaa cccgtcgggg gtgaggacca
     1141 ccgcggaacc ggcgccctgc gcctgccagc cgcccggcac ccgccggctg acccgcaggc
     1201 tcgccaccga ggggagcacc cgctcggcga ccgtgctgac caccatcgag taggcgtcga
     1261 gcgcctcgtc gtcggacggg agatccagcc ccgtacccgc gccgttgcgt cggccgtctg
     1321 tggaggaaat gctgctcacc gctgcctccg gcttgctctg caacatcgta atgttgtaat
     1381 gatgccagct gtccggccat tccgcttgcg cgggtgtacg tgacaggccg gatttgccta
     1441 aactatgcgg ccatgccggc tgatgcggaa ctgctggatg cggttcacct ggcggtccag
     1501 acctccccga ggaacctgac cctgcgcctg cacctcggct acctgctgct tgaggcgggc
     1561 agaccggtcg aggcgctgga gcagtgcgcc ctcgtcctgg tccgggtccc cgaccacggc
     1621 caagcgctgg agctggccac ccgggccgcc gccgacgatg cccggcgcgc ccttccggcg
     1681 ctgccgcggc cgcctcgggc taccgggttc cgggttgagc cggcgctgct caacttcgcc
     1741 agcgtcggcg gcatgaccag cgtcaagcgc cagctcgccg cgatagccga ggtgagcgcg
     1801 agcccgctgc tgtatggccc gccgggctgc ggcaagacct tcatcgccag ggcgctcgcc
     1861 ggcgagctgg gtgcggggtt cctggcggtc aagggcagcc ggatggtcga acggtgggac
     1921 cgcgacggcc gccacgccct ctccgaggcc ttcgcgctgg cgcgggcgag cgtgccgtgc
     1981 ctgctgttcc tcgacgggct ggacgcgctt gcccggagcg cccgcgacgt gaccgaacag
     2041 ctgctcgccg agctggtcgg cgttggcgac caggacggca tcaccgtggt ggcggcctct
     2101 gagcggccct gggagatccc agcgctgctg cgaggccctg gcccagctgg gcgccggagt
     2161 ccagctgccg gccacggcca cggccacggt cagggcctgg gtctgggtct ggggcggggc
     2221 tggggccggg gcggggtcgg cccgcgcctg ctggtgctcc cgcccgaccg cgtggcacga
     2281 caggcgatca tccgcggcgc cctgcgcggc cacccgctcg acgagctgaa cctcgccgcg
     2341 ctcgccggcc ggaccgagta cttctccgcc accgacctga tcgccctctg cgacgtggcg
     2401 gcgcgggtcg ccctgcagac cgcgatgacg cgcggtgtcc ggccgatcgg catggacgac
     2461 ttcgaccgcg ccctgcgcca gctccagcca agcccgctgg cctggttcgg cctcgcccgc
     2521 gactccctgc agcacggcgg cgaggacgcc ctggacatgg acctgcgcgc ctacctgcgc
     2581 accgtgatcc ccaagggcgc cgcctgaggc cccgagcccg aggcttcagg cctggggccc
     2641 ggcgtcaggc cagtcacccc ggcgccccgg cgtcggccac tgcctcggcc tcgatctcga
     2701 cccgccagcg cggatcgagc agggcggcga cgaccaccat ggtggccgcc ggcctgaccg
     2761 ccccgaagat ctcgccatgg gcacgcccga ccgcctccca gtcggccggg tcggtgaggt
     2821 acatccgggt ccggatcacc tgctcggggc cggcgcccgc ctcggccagg gcggcgagga
     2881 tgatctcgag gcagcggcgg gcctgaaccc ccgggtcggg gtcgcaggag ccgtccggcc
     2941 acaccggcgc ggtgcccgag accagcacgc gatcacccac ccgcaacgcc cggcagaagc
     3001 cgatcgtcgg ctcaaaggga gatcccgaag caatgtgggt gcgctggatg gtcatgctcg
     3061 gctccttcga tcggtggctg ccgctggtcg cggtcagctt cacgcgagcg tgaggtcgcc
     3121 ggctaccccc gccgcgaaat tgtggataac gtgcttggcg aggagaggag gccccatgca
     3181 gccgacgcgc tcgttcctgg aatccatcgg cctgcccggc ggtgacgcgt tcgatctgcc
     3241 gacctcggag cggcgcttcc cggacggcgg ccagtaccgc gtcgagatcc cctcggtcga
     3301 gggtccggcc gcgctggaga ccgtcctggc cgaaggcgcc cgccgggggg tgcgcatcca
     3361 ccggatcagc cagggcagcg gcatccagct cctcaccgac gaggagatcg agcggatggt
     3421 ggcgctcggc ggcgagcgcg acgtggaggt gtgcctgttc accgggccgc gggccagctt
     3481 cgacaccggc gtgcaggccg cgaccggctc ggggcgggtg gtcgcgggcg ccctgcgcgg
     3541 tgccgaccag ctcgcgtacg gcgtcgagga cgtgctgcgc gcctgc
//
LOCUS       DASWDC010000345         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_162616, whole genome shotgun sequence.
ACCESSION   DASWDC010000345 DASWDC010000000
VERSION     DASWDC010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162616"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VF995_11015"
     CDS             <1..360
                     /locus_tag="VF995_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9344130.1"
                     /translation="DTLDVIEPLLDGHVDTVAGRLRDGLDSLRANIPDEGAEVRGHGL
                     WLALELGGNGNGGDTANATVSAKEVARAALSRGLVVNPVTETALRIAPPLVIAEAEID
                     EGLARLGGALEEVGACR"
     gene            351..935
                     /gene="argR"
                     /locus_tag="VF995_11020"
     CDS             351..935
                     /gene="argR"
                     /locus_tag="VF995_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX9344131.1"
                     /translation="MSVKVDRQRALAKLLRSRQVGSQARLLELLHLQGFDATQATVSR
                     DLEDLGAIKIRDAEGHPVYALPEAGDSEPGQREELERALTLWLESAVPAGNLVVLRTP
                     PGHAHALAVALDQAAIDDIAGTVAGDDTVLVVCADGISGKAMAHWLRAIGSTSPVPGG
                     ERRGQAARPDDAARPSPTSSTIATTHSIFRGASA"
     gene            1082..2290
                     /locus_tag="VF995_11025"
     CDS             1082..2290
                     /locus_tag="VF995_11025"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX9344132.1"
                     /translation="MVVLAYSGGLDTTVIVHRLTHEHGRRVVALLADVGQGEDLTALA
                     ARALKVGAVDAVVVDARERFARDFLTPAIAANALYERRYPLVSALSRPLIAQLLVEEA
                     RRRGAGALAHGCTGKGNDQVRFEVSCGALAPDLEVLAPVRDWAMSRPEALEYADKYGI
                     DLGWISVDKPYSVDQNLWGRTIEAGVLEDPWSAPPEDAFVWTSSPLIAQGATDVVVSF
                     EAGVPVASDAGSGEGPVHGLEAVLGQLNRLGGGAGFGRVDMIENRRVGIKSREVYEVP
                     GALATIAAHREVEDLTLDRDVLHEKARVELRYAELIYDGLWFSPLRAALDAFIAETQK
                     TVTGEARLQFSPGSCQVTGRRAARQLYRPDLATYAEGDAFDHDAGRGFVALWGLPART
                     WAKVQQEALS"
BASE COUNT          338 a          967 c          865 g          319 t
ORIGIN      
        1 gacaccctcg acgtgatcga gccgctgctg gacggccacg tggacacggt cgccgggcgc
       61 cttcgcgacg gtctcgacag cctgcgggcg aacatcccgg acgagggcgc cgaggtccgc
      121 ggccacggcc tctggctcgc cctggaactg ggcggcaacg gcaacggcgg cgacaccgcg
      181 aacgcgaccg tcagcgcgaa ggaggtcgcc cgggccgcgc tcagccgcgg gctggtcgtc
      241 aatccggtca ccgagaccgc gctgcgcatc gccccgccgc tggtgatcgc tgaggccgag
      301 atcgacgaag gcctcgcccg gctcggcggc gcgctggagg aggtgggcgc atgtcggtga
      361 aggtcgaccg gcagcgggct ctcgccaagc tgctgcgcag ccgccaggtc ggcagccagg
      421 cccgactgct ggaactgctg cacctccagg gcttcgacgc cacccaggcg acggtctcgc
      481 gcgacttgga ggacctcggc gcgatcaaga tccgcgacgc cgaaggccac ccggtctacg
      541 ccctgccgga ggccggcgac tccgaacccg gccagcggga ggagctggag cgggcactga
      601 ccctctggct cgaatctgcc gtgcccgccg gcaacctggt ggtgctgcgc accccgccgg
      661 gccacgcgca cgcgctcgcc gtcgcgctcg accaagccgc gatcgacgac atcgccggga
      721 ccgtcgccgg cgacgacacc gtgctggtcg tctgcgccga cggcatctcc ggcaaagcca
      781 tggcccactg gctgcgtgcc atagggtcga cctccccggt ccccggcggg gagcgccgag
      841 gccaggccgc ccggcccgac gacgccgcgc ggccgtctcc caccagcagc accatcgcca
      901 ccacgcattc gatcttccga ggagcctcag catgaccggt ccgaccgtcc ccaacccggc
      961 ccagccggcc ccgtctcagc ttcctgaccc attggggggc cccgcggcca cagtggaatc
     1021 ggctcgcggt acttccggcc gcgggaccgc gctccccgaa ccccccaacg ccgatcccga
     1081 cctggtcgtg ctcgcctact ccggcggcct cgacaccacc gtgatcgtgc accgcctcac
     1141 ccacgagcat ggccgccggg tcgtagcgct gctcgccgac gtcggccagg gcgaggacct
     1201 gaccgcgctg gccgcccggg cgctcaaggt cggcgcggtc gacgccgtgg tggtcgacgc
     1261 gcgcgagcgg ttcgcgcgcg acttcctcac ccctgcgatc gccgccaacg ccctctacga
     1321 gcggcgctac ccgctggtct cggccctgtc gcggccgctg atcgcccagc tcctggtgga
     1381 ggaggcccgc cgccgtggcg ccggcgctct cgcccacggc tgcaccggca agggcaacga
     1441 ccaggtccgc ttcgaggtca gctgtggcgc cctggccccc gacctcgagg tgctcgcgcc
     1501 ggtgcgggac tgggccatga gccggcccga ggcgcttgag tacgccgaca agtacggcat
     1561 cgacctcggc tggatcagcg ttgacaagcc atattcggtc gaccagaacc tctggggccg
     1621 caccatcgag gcgggcgtgc tggaggaccc ctggagcgct ccgcccgagg acgccttcgt
     1681 ctggacctcc tcgccgctca tcgcccaggg cgcgaccgac gtggtggtct ccttcgaggc
     1741 cggcgtcccg gtcgccagcg acgccggttc gggcgaaggt ccggtgcacg ggctggaggc
     1801 tgtgctcggg cagttgaacc gcctcggcgg cggggccggg ttcgggcggg tggacatgat
     1861 cgagaaccgc cgcgtcggca tcaagagccg cgaggtctac gaggtccccg gcgcgctggc
     1921 caccatcgcc gcccaccgcg aggtcgagga cctcaccctc gaccgcgacg tgctgcatga
     1981 gaaggccagg gtcgagctgc gctacgctga gctgatctac gacgggctct ggttctcgcc
     2041 gctccgcgcc gccttggacg ccttcatcgc cgagacccag aagaccgtca ccggcgaggc
     2101 gcgcctccag ttctcacccg gcagctgcca ggtgaccggc cgccgcgccg cccgccagct
     2161 ctaccgcccc gacctggcca cctacgccga aggcgacgcc ttcgaccacg acgccggccg
     2221 cggcttcgtc gccctgtggg gcctccccgc ccgcacctgg gccaaagtcc agcaggaggc
     2281 cctgtcctga gccagcaagg caaaaccggc ggcgggccgc tgtgggccgg cgggttcgcc
     2341 ggatggcccg acccgtcgat gtgggcctac acctcgtcgc tggcggtcga ccgccagctg
     2401 tggcgcgagg acgtcgccgg cagccgcgcc cacgtccgcg ccctggtcag ggccggcgtg
     2461 atcggcgcgg atgagggcgc cgcgctgct
//
LOCUS       DASWDC010000346        15551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1001568, whole genome shotgun sequence.
ACCESSION   DASWDC010000346 DASWDC010000000
VERSION     DASWDC010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15551
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001568"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="VF995_11030"
     CDS             complement(<1..1331)
                     /locus_tag="VF995_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344133.1"
                     /translation="MPMLTRISHAQARQRTRGLPLLAVLGERIETPAERKLSLEGPGA
                     VWLVERGHLDVFAVARDNGHQRGRLHFLARLPEGAILLGAPPGPAHTLVARPLPGAVV
                     RHIPLGSLLIAQREVWVQNRVAEQLTSEERALAWGIDVGVAAIAEALRDELPPRDFVH
                     LEAARGVELAPGDRARSIDGVLWVRVIGGAVAPSGLAEPLQAGDSIAMSERDWLDSAD
                     GAVVLARSTADLLGAGQLWDLLVGHNQRFLHAIDRRIVTRGQQVAAELAARRQADADT
                     LAHASRTLKAVLEPALAGLPEVIDRDRWLAVARLVGDAEDIEVTAPRGADAADTMLDP
                     LERIALASRFRTRMIRLDPDARWWRTNVGAIAGTIREGGAPVALLWRRRGYQVVDPEA
                     GTRTRVTRAVAEQLQPAGTTFYRPLPVRPSKPFEVLRFALRGSGRDTAHLVL"
     gene            complement(1337..3586)
                     /locus_tag="VF995_11035"
     CDS             complement(1337..3586)
                     /locus_tag="VF995_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="HEX9344134.1"
                     /translation="MTAGAQVETAPVLEPGAGSGSGPRRRWAVPPWRRARRRVKTPTV
                     LQMEAVECGAAALAMVLAYFGRYVPLEELRTACGVSRDGSKAANVLRAARRYGLTAKG
                     MQMDVEHLVSARPPAILFWKFNHFVVFEGRRRLGKPAFFLNDPGSGRRKVSFEELDGC
                     FTGIAMLFDRGPEFQPGGKPVRVIDDLRARRPGTVAAMLIVLVASLLLVACGLAVPAF
                     TRVFVDTVLTNGDQSIVPPLLASMLVVLLLLAGLTAIQQTFLLRAELMTSIVSAARFV
                     RHLLRLPIVFFTQRNAADITRRVSSNDLVAEVLSRDVASAAINAFLVVVYAVLLWTYD
                     VQLTLIGVSMSLLNIAALRWVSRVRKDAVSKLRADRANLVVTSFNGLQLIETMKATGR
                     ENDYFRRWAGFQAKVVSGQQRMGTPSAILSIVAPLLAMLLSALVLLVGGLRAISGHLS
                     IGLLVAFQVLVTSFTRPVTQLTNLGPKIQDLAADVTRLRDVERFPVAPVFETPVPPTG
                     RRLDGHLEFDAVTFGYSPLADPLLKEFSFQIGPGQRIALVGGSGSGKSTVSRLVAGLY
                     EPWAGAIRFDGKAREELPRDTLAASVAFVDQDIFLFEGSVRDNVTLWDPTISDEAVVN
                     ALKNASIYDEVASRPGSLNSMVSDSGRNFSGGQRQRLEIARALVRSPSVLVLDEATSS
                     LDAETEKRIDDNLRRRGCACVIIAHRLSTIRDADEILVLEGGRIVQRGRHEQLIGCDG
                     PYADLLREL"
     gene            complement(3608..4420)
                     /locus_tag="VF995_11040"
     CDS             complement(3608..4420)
                     /locus_tag="VF995_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX9344135.1"
                     /translation="MEFRPKALSSLRSPDELDTAVRLTGARAWLVVLAFLVVVGLGAT
                     WAFAGEVPRSVSAPGILTHPLGVSAVQSTVSGLVSKVFPQTGSVVVAGTPIATIVTGD
                     GQTQVVRVPFSGQVTNVLVTSGQYVTVGTAVILVERTDDPHDRLLAMLYAEADKAAFI
                     RPGDQVDLEVSAAPSAAFGVLRGTVTAVGQFSQTRRQVAGFLGNDQLAGKFSAAGSPI
                     AVMVDLSIDPRTVSGLKWSTGTGPPYRIDSQVEVTALVHLPSQRPIDWVLPS"
     gene            complement(4404..5945)
                     /locus_tag="VF995_11045"
     CDS             complement(4404..5945)
                     /locus_tag="VF995_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HEX9344136.1"
                     /translation="MPIPPPDPETAAGLREVVQLRQGVTLAATDSTATLLVQLRGERF
                     AGLSPGLQAVIERLRKEPASEAELTELVGRHDGEAGVLGLHGLLARLRRGGWLETTLE
                     LERVEGARPLLSILPHAPHPLVGREDAGAAEQVVLSRFTVLRRDDDRLVAESPRAKAT
                     LTFHDPAVAELVCGLVRPQRPGALPGTGAMPAAAASRVLRTLLGYGFAVAAGDAEETE
                     LRLRQWSTHEAWFHSRVRMGRHDLPYGGTYWAKELVPPLPGRHEPASASASASGSGSG
                     SGSGGGSSGRRLALARPDLERLRVAEPTLTEVLEERRSIREHDDRHPITLAQLGEFLF
                     RSARVISVRPGEAQELTRRPYPSGGSVYELELYPVVANVAGLAAGMYRYDPYDHALEA
                     LPATEHAVRRLLMVGRRIGVMEATPQVLIVVAARFGRFMWKYQTMAYATILKHVGVVY
                     MTMYCVATAMGLAPCGLGGGDAEDFAAATGLDFFAESSVGEFLLGSRVPPERSREVPK
                     YGIPA"
     gene            complement(5986..8325)
                     /locus_tag="VF995_11050"
     CDS             complement(5986..8325)
                     /locus_tag="VF995_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HEX9344137.1"
                     /translation="MSASGMERPRLKAHLKPFTLGDRVFLVAEQRHYLVQGAAAVAVL
                     PYLDGRHTVGEIVTALEGEVSFADLVLALTKYQRFGHLADGLDGHGDRLDVRAVAAWD
                     LRGIDPVAAAGQLAATTVAVTTVGDADPGEVVAALRETGMTVRLADWDEAGADPGAAL
                     AVVVTDEYLDPLLGKLDAAFRAADRRWLLVKPIGAEVWAGPCFVPGETGCWQCLAQRL
                     DGNRMVEQYLRFRSPSDLGVIRVSLAALPPSRQLAAALVAGAVAELAATGACPALEGT
                     IVSLHAQTLQLAHHRLIRQPQCPGCGDPALTRERPARVALRSQPVAFGREGGHRVMRP
                     DETYRRLERHISPITGVVRELSSLNDADEGITYSYAAGHNFALPSDNIAMLRRNLRGQ
                     SGGKGRTDIQARVSGMCEAIERYSGVWRDDRPTVQGSFDELGPDQAVFLPELLGYSPA
                     QYAERIRWNQSSGGRYNQVPRPYDPSQPIAWTPAWSLTRQRERLVPAAYAWFGHPDLY
                     RHLFCFPDGNGNSSGNVLEEAVLQAFCELVERDAVALWWYNRLRRPGVDLDRLHDPYI
                     DQLRDYYDANRRSLWVLDITSDLGVPTFAAVSHRLDHPVQDVILGFGAHLDRRLGLLR
                     ALTELNQFLPIVSQRFPDGSTIYWEDDEYVLEWWKTARVEQEPWLRPAPGASARVAGA
                     NRGPGPGPGDSDGRDGADLRDEIETCLARAGAAGLEVLVLDQSRPDIELNVAKVIVPG
                     MRHFWRRLGPGRLYDVPVRLGWLEAPHREQDLNPRSVFF"
     gene            complement(8378..8812)
                     /locus_tag="VF995_11055"
     CDS             complement(8378..8812)
                     /locus_tag="VF995_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344138.1"
                     /translation="MQFGISLLPDASPQTKPATDYYRDVLRLCRVADEAGFGYVKMTE
                     YFRVWLDATESWNATRSKDYQSYTGMSYFLRGLTPTAWREAGSAMVGSPERIVDRLHE
                     FQEAAGLDTIFWQVDFGAVGGQLAERSLRLFIDKVLPKVSDL"
     gene            complement(8881..9729)
                     /locus_tag="VF995_11060"
     CDS             complement(8881..9729)
                     /locus_tag="VF995_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9344139.1"
                     /translation="MSTANPLAAPAERPLPGVLRVGLSHAKVEVLNEVLNYVRNPIAV
                     IFTFALPIVLLVFIGAMYSGTVAGTGVSVKKVLVAGILAAGIMSTTFSGLAIGIAAER
                     SEGTLHRLAGTPMPRAAYFLGKLINIYAVTLLEVLALLVVGTTLYHLSWPATAGHWVT
                     LGWVVLLGTTACATLGIAWSRMAPNARVATPVVQLPFLSMQFISGVFVPFSSLPKALQ
                     VVAALFPLKWLAQGLRSVFLPAAFQHAEVAGSWELGRVAVVLGAWTVGAFAVALFTFT
                     WGGPEE"
     gene            complement(9726..10679)
                     /locus_tag="VF995_11065"
     CDS             complement(9726..10679)
                     /locus_tag="VF995_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9344140.1"
                     /translation="MSSAAIAVRGLRKRYGRLEVLRGINLEIQAGEVFALLGPNGAGK
                     TTTVEILEGFRRREGGEASVLGADPARAGRRWRAQIGIVPQDTGAFEELTVSELVAHF
                     RSFYPDPLGTDQVIGVVGLRDKAGERAAKLSGGQRRRLAIALGIVGNPRLIFLDEPTT
                     GLDPAIRRQTWELIRELAAREVTILLTTHYLDEVEALANRVAVLSGGRIVAEGRPESL
                     GGRASGEAHVSFSLPHMLAGLDLPPGLPEAAVHEGLVTLRTAEPTDAVAKLIVWARQH
                     GVRELPGLSVARPSLEDVYLRLISSGPRRSTAETAPEGALA"
     gene            complement(11126..12070)
                     /locus_tag="VF995_11070"
     CDS             complement(11126..12070)
                     /locus_tag="VF995_11070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9344141.1"
                     /translation="MALLLIVQSSDQARSDTAATLRAEGHEVLEAASAEDAVSLSRAW
                     RPAVILLDPLLPGETGDDLYVTLQRELAGLATQILFLPVPPLPPPAAGAEAEAGAEAA
                     GPVLPEPLLGGLELVTRVGLALRAKAVHDQLDEADTALPRQLPGGLQSGGAGWTDPLT
                     GLANRRACAERLAAACRRAAAGAEPVALVLADLDHFARVNEAVGYAAGDQALQLFAGL
                     LHESCRGGDTAGRWEGATWVLILPGIGLEPAWYVAERVRLRTAALTKAIGTERVALSA
                     SLGVAEYQEGDLWTDLFGRAETALARAKRSGRNRAEVA"
     gene            12201..12968
                     /gene="tsaB"
                     /locus_tag="VF995_11075"
     CDS             12201..12968
                     /gene="tsaB"
                     /locus_tag="VF995_11075"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX9344142.1"
                     /translation="MLVLGVDTSTPVTSVAVGTDAGPLASAAVRNDRAHAELLAPLVA
                     WTLEQAGIDGSALGGVAVGVGPGLFTGLRVGVSGAKALAQAWRLPMVAVPSLDLLAFA
                     QRHAKRTICAMIDARRGELFAALYRPVHGGVVRVTDYQVIRPDQLAAGLQARGGSVLL
                     CGDGALRYHSAFEHLGEDVEVTGPGWAAPSAVALVELALLRFHREEFTHPFDVSPLYL
                     RRPDVDPAVERRLAAEHHAAKGDAANAAAGTHEAAGA"
     gene            12971..13540
                     /gene="rimI"
                     /locus_tag="VF995_11080"
     CDS             12971..13540
                     /gene="rimI"
                     /locus_tag="VF995_11080"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX9344143.1"
                     /translation="MATLFPPRSGRLDDPDVPLDVELTQMRRRHIPQVLDIERQVYPR
                     PWTMTLFLSEIVQRSTRFYIVARASRRVVGYAGLMIFGDEAHVTNIAVDPAAHRRKIA
                     SRLLYALVTEARCRGATACTLEVRVANHGAQGLYHQFGFAPVGIRKNYYAETGEDALI
                     MWAEGLQTPAYETRLAELARRIPEPREAR"
     gene            13545..14621
                     /gene="tsaD"
                     /locus_tag="VF995_11085"
     CDS             13545..14621
                     /gene="tsaD"
                     /locus_tag="VF995_11085"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX9344144.1"
                     /translation="MLILGIETSCDETGVALVEDGTVVRSNVIADQQTLHARYGGVVP
                     EVASRAHVDLLHPAVEQACALGGVHLGDVDAIAVTIGPGLAGALLVGVAAAKSYALAL
                     DRPLLGVNHLIGHVYANVLDHGALPLPAVVFVVSGGHTSLLVMDEQGAFRVLGQTIDD
                     AAGEAYDKVARVLGLGYPGGPVIDRLAREGDPAAVRFPRALIDDGTYDFSLSGLKTAV
                     LRHVRAHRVDSGEKLNVNDVAASFQEAVVDVQVSKILRAAAEAGIDTVLLAGGVAANS
                     RLRARLSEEAERRDVRVLAPSPALCTDNGAMIACAGWFQLKRGERTPLDVGADPNLQI
                     VPGVGRRASRRPKRRGGTTSTRHE"
     gene            14734..15027
                     /gene="groES"
                     /locus_tag="VF995_11090"
     CDS             14734..15027
                     /gene="groES"
                     /locus_tag="VF995_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9344145.1"
                     /translation="MGVSLQPLEDRIVVKPGEGEETTASGLVIPDTAKEKPQEGEVVA
                     VGPGRFEDGKRIPLDVSVGDTVIYSKYGGTEVKYGGEDYLILSARDVLAIVKK"
     gene            15048..>15551
                     /locus_tag="VF995_11095"
     CDS             15048..>15551
                     /locus_tag="VF995_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX9344146.1"
                     /translation="MAKQLLFGTDARRALEAGVDKLANVVRVTLGPKGRNVVLDKKWG
                     APTITNDGVTIAKEIDLEDNYENLGAQLAKEVATKTNDVAGDGTTTATVLAQALVHEG
                     LRNVAAGANPMGLKRGIERAVEAVAEHIRNQARELETQEEIASVAAISAQDAEIGRII
                     AEAMDKVG"
BASE COUNT         2199 a         5581 c         5741 g         2030 t
ORIGIN      
        1 agcaccaggt gggcggtgtc gcggcccgac ccgcgcaggg cgaagcgcag cacctcgaac
       61 ggtttcgagg gccgcaccgg cagcggtcgg tagaaggtcg tcccggccgg ctggagctgc
      121 tcggcgaccg cgcgggtcac ccgcgtccgg gtgcccgcct ccgggtcgac cacctggtag
      181 ccgcgtcgcc gccacagcag cgccaccggc gccccgccct cacgaatggt gccggcgatc
      241 gcgcccacgt tggtgcgcca ccagcgggcg tcggggtcca ggcggatcat ccgggtgcgg
      301 aagcgggagg cgagcgcgat ccgctccagc gggtcgagca tcgtgtcggc ggcgtcggcg
      361 ccccgcggcg cggtcacctc gatgtcctcg gcgtcgccga ccagccgggc gaccgccagc
      421 cagcggtccc ggtcgatgac ctccggcagt ccggcgagcg ccggctccag gaccgccttg
      481 agggtccgcg acgcgtgcgc gagggtgtca gcgtcagcct ggcggcgggc ggccagctcc
      541 gcggcgacct gctggccgcg ggtgacgatg cgccggtcga tcgcgtgcag gaagcgctgg
      601 ttgtggccga ccagcaggtc ccagagctgc ccggcgccga gcaggtcggc ggtggagcgg
      661 gccagcacca ccgcgccgtc ggcgctgtcg agccagtcgc gctcgctcat cgcgatcgag
      721 tcgcccgcct gaagcggctc cgccagcccg ctgggggcca ccgcgccacc gatcacccga
      781 acccacagca cgccgtcgat ggagcgagcg cggtcgccgg gcgccagctc cacgccgcgt
      841 gcggcctcca ggtggacgaa gtcgcgcggc ggcagctcgt cgcgcagcgc ctcggcgatc
      901 gcggccacgc ccacgtcgat gccccaggcg agggcgcgct cctcgctggt gagctgctcg
      961 gcgacccggt tctggaccca cacctcccgc tgggcgatca gcagggagcc gagcgggatg
     1021 tggcgcacca ccgcgccagg cagcggccgg gcgaccagcg tgtgggccgg tccgggcggc
     1081 gcccccagca ggatcgcgcc ctcgggcagg cgcgccagga agtgcagccg cccgcgctgg
     1141 tggccgttgt cgcgcgccac cgcgaagacg tcgaggtggc cgcgttccac cagccagacc
     1201 gcgccgggtc cctccagtga gagcttgcgc tcggcggggg tctcgatccg ttcgccgagc
     1261 acggcgagca gcggcaggcc tcgggtgcgc tgccgcgcct gcgcgtggga gatgcgggtc
     1321 agcatcggca tgcggatcac agctcccgca gcaggtcggc gtaggggccg tcgcagccga
     1381 tcagctgctc gtggcggccc cgctggacga tgcggccgcc ttcgagcacg aggatctcgt
     1441 cggcgtcgcg gatggtggac agccggtgcg cgatgatcac gcaggcgcag ccgcggcgcc
     1501 gcaggttgtc gtcgatgcgc ttctcggtct cggcgtccag cgagctggtc gcctcgtcca
     1561 ggaccaggac gctgggggag cgcaccagcg cacgggcgat ctccaggcgc tggcgctgcc
     1621 cgccggagaa gttgcggccg ctgtcggaga ccatgctgtt gaggctgccg gggcggctgg
     1681 ccacctcgtc gtagatgctg gcgttcttga gcgcattcac caccgcctcg tcgctgatgg
     1741 tcgggtccca cagcgtgacg ttgtcgcgca cgctcccctc gaacaggaag atgtcctggt
     1801 cgacgaaggc gaccgaggcc gccagcgtgt cgcggggcag ctcctcgcgg gccttgccgt
     1861 caaagcggat cgcgccggcc cagggctcgt acaggccagc gaccaggcgg ctgacggtgg
     1921 acttgccgct gcccgacccg cctaccaagg cgatccgctg gcccgggccg atctggaagg
     1981 agaactcctt gagcagcggg tcggccaggg ggctgtagcc gaaggtgacg gcgtcgaact
     2041 cgaggtggcc gtcgaggcgg cggccggtgg gcgggaccgg ggtctcgaac accggcgcga
     2101 ccgggaagcg ctccacgtcg cgcagcctgg tcacgtcggc ggccaggtcc tggatcttgg
     2161 ggcccaggtt ggtgagctgg gtgaccggcc gggtgaagct ggtgaccagg acctggaagg
     2221 cgaccagcag gccgatcgac agatgccccg atatcgcccg caagccgccg accagcagga
     2281 ccagcgcgct gagcagcatg gccagcagcg gtgccacgat cgacaggatc gcgctcggcg
     2341 tccccatccg ctgctggcct gagaccacct tggcctggaa gccggcccag cggcggaagt
     2401 agtcgttctc gcggccggtc gccttcatcg tctcgatcag ctgcaggccg ttgaaggagg
     2461 tgacgaccag gttggcgcgg tcggcgcgga gcttggacac cgcgtccttg cggacccgcg
     2521 agacccagcg cagcgccgcg atgttcagca gcgacatcga cacgccgatg agggtcagct
     2581 gcacgtcgta ggtccacagc aggaccgcgt acaccaccac caggaaggcg ttgatggccg
     2641 ccgaggcgac gtcgcgcgac agcacctcgg cgaccaggtc gttggaggag acccggcggg
     2701 tgatgtcggc ggcgttgcgc tgggtgaaga acacgatcgg caggcgcagc aggtggcgga
     2761 cgaagcgcgc ggcgctcacg atcgaggtca tcagctcggc gcgcagcagg aaggtctgct
     2821 ggatcgcggt cagcccggcc agcagcagca gcaccaccag catcgaggcg agcagcggcg
     2881 gcacgatcga ctggtcgccg ttggtcagga cggtgtcgac gaacacccgg gtgaaggcgg
     2941 gcacggcgag gccgcaggcg accagcagca ggctcgcgac cagcacgatg agcatcgcgg
     3001 cgacagtgcc cggccggcgg gcgcgcaggt cgtcgatcac ccgcacgggc ttgccgccgg
     3061 gctggaactc cgggccgcgg tcgaacagca tggcgatgcc ggtgaagcag ccgtcgagtt
     3121 cctcgaagga gaccttgcgg cggccgctgc cggggtcgtt gaggaagaag gccggcttgc
     3181 cgaggcgccg gcggccttcg aagaccacga agtggttgaa cttccagaac aggatcgccg
     3241 gcgggcgcgc gctgacgaga tgctcgacgt ccatctgcat gcccttggcg gtgaggccgt
     3301 agcggcgcgc cgcgcgcagc acgttggctg ccttggagcc gtcgcgggac acgccgcagg
     3361 cggtgcgcag ctcctccagc ggcacgtagc gcccgaaata ggccagcacc atcgccagcg
     3421 cggcggcgcc gcactcgacc gcctccatct gcagcaccgt cggggtcttc accctccgcc
     3481 tggctcgccg ccagggcggc acggcccagc gccggcgcgg ccccgagccg gagccggcgc
     3541 cgggctccag caccggtgcg gtctccacct gcgctcccgc ggtcatctcg gtctgtcccc
     3601 tctcagctca gctcggcagc acccagtcga taggccgctg gctgggcagg tgcaccagcg
     3661 cggtcacctc gacctgggag tcgatccggt agggggggcc ggtgcccgtc gaccacttga
     3721 ggccgctcac cgtgcgcggg tcgatgctca ggtcgaccat caccgcgatc ggcgagccgg
     3781 ccgcgctgaa cttgccggcg agctggtcgt tgccgaggaa gcccgcgacc tggcggcgtg
     3841 tctgggagaa ctgcccgacc gcggtgacag tgccgcgcag caccccgaag gcggccgacg
     3901 gcgccgcgct gacctccagg tcgacctggt cgcccggccg gatgaaggcg gccttgtcgg
     3961 cttcggcgta gagcatcgcc agcaggcggt cgtgggggtc gtcggtgcgc tcgaccagga
     4021 tcacggcggt gccgacggtg acgtactggc cgctggtgac cagcacgttg gtgacctgtc
     4081 ccgaaaacgg cacccgcacc acctgggtct ggccgtcgcc ggtcacgatc gtggcgatcg
     4141 gggtgcccgc caccaccacg ctgccggtct gggggaacac cttggagacc agcccgctga
     4201 ccgtgctctg cacggcgctg acgcccagcg ggtgggtcag gatgcccggc gcgctgacgc
     4261 tgcgcggcac ctccccggcg aacgcccagg tggcgccgag gccgaccacc accaggaagg
     4321 ccaacaccac cagccaggcg cgggcgccgg tgaggcggac ggcggtgtcc agctcgtcgg
     4381 gggagcgcag gctgctgagc gccttaggcc ggaattccat acttgggcac ctctcgtgac
     4441 cgctccggcg gcacccggct gccgagcagg aactcgccga cgctggactc ggcgaagaag
     4501 tcgaggccgg tggcggcggc gaagtcctcg gcgtcgccgc cgcccagccc gcacggcgcc
     4561 agccccatcg ctgtcgccac gcagtacatc gtcatgtaga ccaccccgac gtgcttgagg
     4621 atggtggcgt aggccatcgt ctggtacttc cacatgaagc ggccgaagcg ggccgcgacc
     4681 acgatcagca cctgcggggt ggcctccatc accccgatcc ggcgaccgac catcagcagc
     4741 cggcgcaccg cgtgctcggt cgccgggagc gcctccagcg cgtggtcgta ggggtcgtag
     4801 cggtacatcc cggcggccag cccggcgacg ttggcgacca ccgggtacag ctccagctcg
     4861 tagaccgatc cgcccgacgg gtagggccgg cgggtcagct cctgcgcctc gcccggccgc
     4921 accgagatga cccgcgccga gcggaacagg aactcgccga gctgcgccag ggtgatcggg
     4981 tgccggtcgt cgtgctcgcg gatcgagcgg cgctcctcca gcacctcggt cagggtcggt
     5041 tcggcgaccc gcagccgctc cagatcgggg cgggccagcg ccagccgccg cccgctgctg
     5101 ccgccgccgc tgccgctgcc gctgccgctg ccggaggcgg aggcggaggc ggaggcgggc
     5161 tcgtggcggc cgggcagcgg cgggaccagc tccttggccc agtaggtgcc gccgtagggc
     5221 aggtcgtggc ggcccatgcg gacgcgggag tggaaccacg cctcgtgcgt gctccactgc
     5281 cgcagccgca gctcggtctc ctcggcgtcg ccggccgcga ccgcgaagcc gtacccgagc
     5341 agggtccgca gcacccggct cgccgccgcc gctggcatcg cgccggttcc gggcagggcg
     5401 cccggccgct gcgggcggac caggccgcag accagctcgg cgacagccgg gtcgtggaac
     5461 gtcagggtgg ccttcgcccg gggcgactcg gccaccaagc ggtcgtcgtc gcggcgcagc
     5521 accgtgaagc gggacagcac cacctgctcg gccgcgcccg cgtcctcgcg gccgaccagc
     5581 gggtggggcg cgtgcggcag gatcgacagc agcggccggg cgccctccac ccgctccagc
     5641 tccagggtcg tctccagcca gccgccccgg cgcagccggg cgagcaggcc gtgcaggccc
     5701 agcacgccgg cctcgccgtc gtggcggccg accagctcgg tcagctcggc ctcgctggct
     5761 ggctccttgc gcagccgctc gatgaccgcc tgcaggccgg ggctgagccc ggcgaaccgc
     5821 tcgccgcgca gctgcaccag cagggtggcg gtgctgtcgg tcgccgccag ggtcacgccc
     5881 tgccgcagct gcacgacctc gcggagaccg gcggcggtct cgggatcggg tggcgggatg
     5941 ggcacgtgaa gctcctttgt ggtccgtgtg gtccgtgccc gcacttcaga agaagacgct
     6001 gcgggggttg aggtcctgct cgcggtgggg cgcctccagc cagccgagcc gcaccggcac
     6061 gtcgtagagc cgtcccggcc cgaggcggcg ccagaagtgg cgcatccccg gcacgatcac
     6121 cttggcgacg ttcagctcga tgtcggggcg gctctggtcc agcaccagca cctccagccc
     6181 ggccgccccg gcccgcgcca ggcaggtctc gatctcgtcg cggaggtcgg cgccgtcgcg
     6241 gccgtcgctg tcgccggggc cgggcccggg cccgcgattc gccccggcga cgcgcgctga
     6301 ggcgcccggg gcgggccgca gccacggctc ctgctcgacc cgggcggtct tccaccactc
     6361 caggacgtac tcgtcgtcct cccagtagat ggtgctgccg tcggggaagc gctggctcac
     6421 gatcggcagg aactggttca gctcggtgag ggcgcgcagc agccccagcc gccggtcgag
     6481 gtgggcgccg aacccgagga tcacgtcctg gacggggtgg tcgagccggt gcgagaccgc
     6541 ggcgaaggtg gggacgccga ggtcgctggt gatgtccagg acccagaggc tgcgccggtt
     6601 cgcgtcgtag tagtcgcgca gctggtcgat gtaggggtcg tggaggcggt cgaggtcgac
     6661 gcccggccgg cgcaggcggt tgtaccacca cagcgccacc gcgtcgcgtt cgaccagctc
     6721 gcagaacgcc tgcagcaccg cctcctccag cacgttgccg gaggagttgc cgttgccgtc
     6781 ggggaagcag aacaggtgcc ggtagaggtc ggggtggccg aaccaggcgt aggcggcggg
     6841 gaccagccgc tcccgctggc gggtcagcga ccaggccggc gtccaggcga tcggctggct
     6901 ggggtcgtag gggcggggga cctggttgta gcgtccgccg gacgactggt tccagcggat
     6961 ccgctccgcg tactgcgccg gcgagtagcc gagcagctcc ggcaggaaca ccgcctggtc
     7021 ggggccgagc tcgtcgaagc tgccctggac ggtgggacgg tcgtcgcgcc agacgccgct
     7081 gtagcgctcg atcgcctcgc acatgccact gacccgcgcc tggatgtcgg tgcggccctt
     7141 gccgccgctc tggccgcgga ggttgcggcg cagcatggcg atgttgtcgc tgggcagggc
     7201 gaagttgtgc ccggcggcgt agctgtaggt gatgccctcg tcggcgtcgt tgagcgagct
     7261 gagctcccgc accaccccgg tgatcgggct gatgtggcgc tccagccgcc tgtaggtctc
     7321 gtcggggcgc atcacccggt ggccgccctc gcggccgaac gcgaccggct gggaccgcaa
     7381 cgccaccctg gcgggccgct cgcgggtgag cgccgggtcg ccgcagccgg ggcactgcgg
     7441 ctggcggatc agccggtggt gggcgagctg gagggtctgg gcgtggaggc tgacgatggt
     7501 gccctccagc gccgggcagg cgccggtcgc ggccagctcg gccaccgcgc cggccaccag
     7561 ggcggcggcg agctgccgcg agggcggcag cgccgccagc gacacgcgga tgacccccag
     7621 gtcggagggc gagcggaagc ggagatactg ctccaccatg cggttgccgt ccaggcgctg
     7681 cgccaggcac tgccagcagc cggtctcgcc cgggacgaag caggggcccg cccagacctc
     7741 ggcgccgatc ggcttgacca gcagccagcg ccggtcggcg gcgcggaagg ccgcgtccag
     7801 cttgccgagc agcgggtcga ggtactcgtc ggtcaccacc accgccagcg ccgccccagg
     7861 atcggccccg gcctcgtccc agtcggcgag ccggacggtc atcccggtct cgcgcagggc
     7921 cgccaccacc tcgccggggt cggcgtcacc gacggtggtg acggcgacgg tggtcgcggc
     7981 cagctgcccg gccgccgcca ccgggtcgat cccgcgcagg tcccaggcgg ccacggcgcg
     8041 gacgtccagg cggtcgccat gaccgtcgag gccgtcggcg agatgcccga accgctggta
     8101 tttggtgagg gcgagcacca ggtcggcgaa cgacacctcg ccctccagcg cggtcacgat
     8161 ctccccgacc gtgtggcgcc cgtccaggta gggcagcacc gccaccgccg ccgcgccctg
     8221 caccaggtag tgccgctgct cggcgaccag gaacacccgg tccccgaggg tgaagggctt
     8281 caggtgggcc ttgagcctcg gccgctccat cccgctggcg ctcattcggc acgctccggc
     8341 atcgtcgtcc gtcgccgcgg cgcgcccacc gtggttctca tagatctgag accttcggaa
     8401 ggaccttgtc gatgaacagg cgcaggctgc gttcggccag ctggccgccg accgcgccga
     8461 agtcgacctg ccagaagatc gtgtcgaggc cggcggcctc ctggaactcg tggaggcggt
     8521 cgacgatccg ctccggcgag ccgaccatgg cgctgcccgc ctcccgccag gcggtcgggg
     8581 tgaggccgcg caggaagtag ctcatgccgg tgtaggactg gtagtccttg gagcgggtcg
     8641 cgttccacga ttcggtggcg tcgagccaga cccggaagta ctcggtcatc ttgacgtagc
     8701 cgaagccggc ctcgtcggcg acgcggcaca gccgcagcac gtcgcggtag tagtcggtgg
     8761 cgggcttggt ctgcgggctg gcgtccggca ggagcgagat tccgaactgc atggtgcctc
     8821 ctgtacgggt ggcggcgggg ccggaccggc ggcattgggg gcgggggttc ggggcttacg
     8881 tcactcctct ggacctcccc aggtgaaggt gaacagggcg acggcgaagg cgccgacggt
     8941 ccaggcgccg aggaccacgg cgacacggcc gagctcccag ctgccggcga cctcggcgtg
     9001 ctggaaggcg gcgggcagga acaccgagcg cagcccctgc gcgagccact tgagcgggaa
     9061 cagcgccgcg accacctgca gcgccttggg cagggagctg aaggggacga agaccccgga
     9121 gatgaactgc atcgacagga acgggagctg caccaccggg gtggcgacgc gggcgttggg
     9181 cgccatccgc gaccaggcga tcccgagggt cgcgcaggcg gtggtgccga gcagcaccac
     9241 ccacccgagg gtgacccagt gcccggcggt cgccggccag ctcaggtggt acagggtggt
     9301 gccgacgacc agcagcgcca gcacctcgag cagggtgacg gcgtagatgt tgatcagctt
     9361 gccgaggaag taggcggcgc gcggcatcgg cgtgcccgcc aggcggtgga gggtgccctc
     9421 gctgcgttcg gcggcgatgc cgatcgccag gccgctgaag gtggtgctca tgatgccggc
     9481 ggcgaggatc ccggcgacca ggaccttctt gaccgacacc ccggtgccgg ccactgtgcc
     9541 gctgtacatc gcgccgatga acaccagcag cacgatcggc agggcgaagg tgaagatcac
     9601 cgcgatcggg ttgcgcacgt agttgaggac ctcgttgagg acctcgacct tggcatggga
     9661 gaggccgacc cggaggacgc cggggagggg ccgctcggcg ggcgccgcga gcgggttggc
     9721 ggtgctcatg ccagcgctcc ttccggtgcc gtctccgccg tcgaccgccg cgggccgctg
     9781 ctgatcaggc ggagatagac gtcttccagc gagggccgcg ccacgctcaa gccgggcagc
     9841 tcccggacgc cgtgctggcg ggcccagacg atcagcttgg cgaccgcgtc ggtgggctcg
     9901 gccgtgcgga gggtgaccag gccctcgtgc acggccgcct cgggaaggcc gggcggcagg
     9961 tccaggccgg cgagcatatg ggggagcgag aagctcacgt gcgcctcgcc gctggcgcgc
    10021 ccgcccaggg actcgggcct gccttcggcg acgatcctgc cgcccgacag caccgcgacg
    10081 cggttggcga gcgcctcgac ctcgtcgagg tagtgggtgg tgagcaggat ggtgacctcg
    10141 cgggcggcca gctcgcggat cagctcccag gtctgccgcc ggatcgccgg gtccagcccg
    10201 gtggtcggct cgtcgaggaa gatgagccgc gggttgccga cgatgccgag cgcgatcgcc
    10261 aggcgccgcc gctggccgcc ggacagcttg gcggcccgct ccccggcctt gtcccgcagg
    10321 ccgaccacgc cgatcacctg gtcggtgccg agcgggtccg ggtagaagct gcggaagtgg
    10381 gcgaccagct cgctgacggt cagctcctcg aaggcgccgg tgtcctgggg gacgatgccg
    10441 atctgggcgc gccagcggcg tccggctctg gcggggtccg cgccgagcac cgacgcctcg
    10501 ccgccctcgc ggcggcggaa cccttcgagg atctcgacgg tggtggtctt gccagcgccg
    10561 ttcggcccga ggagggcgaa cacctccccg gcctggatct cgaggttgat cccccggagg
    10621 acctcgagcc ggccgtagcg cttgcggagc ccccggacgg cgatggccgc gctggacatg
    10681 gcaacacctc gaatccgccc agatgccccg ggatggaggt tcggagtcgc ggtgcgcgcg
    10741 cgcgaacgtt gtccttgtca ggggaccaat gcgccattac tgtggagacg cgcacgtgct
    10801 tcctggctgc atcccggagc gtgcctggta ggagtgagcc tggcgttgcg acgatccaga
    10861 ccttcgtcgc agcgtcaggc tcatggttgt agcggcggaa cctgacgacg aattcgctta
    10921 tccagcttat ttctgacttt ttcggcggat cagacctggc gcgcaaaggc tgtgtaacag
    10981 gaggctacgg aacgcccgtg agctgggcaa ccccgaaatt aggggacttg aacccgtagg
    11041 cgttttccgc taccacccca atacacctcg gcgtcgctac gtatggtggc cgcgcccgta
    11101 cgctacgccg ctgagttcgt ggcgcctacg ccacctcggc gcggttgcgg ccggagcgtt
    11161 tggcacgggc cagggcggtc tcggcgcggc cgaacaggtc ggtccacagg tcgccctcct
    11221 gatactcggc gacccccagg gaggccgaga gggcgacccg ttcggtgccg atcgccttgg
    11281 tgagcgcggc ggtgcgcagc cgcacccgct cggccacgta ccaggccggc tccagcccga
    11341 tccccggcag gatcagcacc caggtcgcgc cctcccagcg ccccgccgtg tcgccaccgc
    11401 ggcaggactc gtgcaacagg ccggcgaaca gctgcagcgc ctggtccccg gcggcgtatc
    11461 cgaccgcctc gttgacccgc gcgaagtggt ccaggtcggc cagcaccagc gcgaccggct
    11521 cggcgccggc cgccgcccgc cggcacgccg ccgccaggcg ctccgcgcag gcgcgccggt
    11581 tggcgagccc ggtgagcggg tcggtccacc ccgccccgcc gctctggagg ccgccgggga
    11641 gctggcgggg gagcgccgtg tcggcctcgt cgagctggtc gtgcaccgct ttcgcgcgca
    11701 gcgccaaccc gacccttgtg accagctcca ggccgccgag cagcggctcc ggcaggaccg
    11761 gcccggccgc ctccgcgccg gcctccgcct ccgcgccggc ggcgggaggc ggcagcggtg
    11821 ggaccgggag gaacaggatc tgcgtggcaa gcccggcaag ctcgcgctgc agcgtcacgt
    11881 agaggtcgtc gccagtctcc ccgggcagca gcgggtcgag caggatgacc gccggccgcc
    11941 acgccctcga caaggagacg gcatcctcgg cggaggcggc ctccagaacc tcgtggccct
    12001 cggcccgcag ggtcgccgcc gtgtccgacc gggcctggtc ggacgactgg acgatcagga
    12061 gcagcgccat cccctcctga atcggcctcc gtccgccacg ggctgagcgc gggcggggac
    12121 acacctgggc gcggatggcg gggacggtgc cacaatacgg gttcctgacc gaccgctccg
    12181 accgcttgaa aggcgcatcg ttgctcgtcc tgggggtcga caccagtacg ccggtgacca
    12241 gcgtcgccgt gggcaccgac gcgggcccgc tggcgtcggc ggccgtgcgc aacgaccgtg
    12301 cccatgccga gctgctggcc ccgctggtcg cctggacgct ggagcaggcc gggatcgacg
    12361 gctccgccct cggcggggtc gccgtcggcg tcggcccagg gctgttcacc ggcctgcggg
    12421 tgggggtcag cggcgccaag gcgctggcgc aggcgtggcg gctgccgatg gtggcggtgc
    12481 cgagcctgga tctcctggcg ttcgcgcagc gtcacgcgaa acggacgatc tgcgcgatga
    12541 tcgacgcgcg gcgcggcgag ctcttcgccg ccctctaccg gccggtccac ggcggggtcg
    12601 tgcgggtgac cgactaccag gtgatccgcc ccgaccagct cgccgccggg ctgcaggcgc
    12661 ggggcggcag cgtgctgctg tgcggcgacg gcgccctgcg ctaccacagc gccttcgagc
    12721 acctcggcga ggacgtcgag gtgacaggac ccggctgggc cgcgccctcg gcggtggcgc
    12781 tggtggagct ggcgctgctg cgcttccacc gcgaggagtt cacccatccc ttcgacgtgt
    12841 ccccgctgta cctgcgccgc cccgacgtcg atccggcggt cgagcgccgg ctggcggccg
    12901 agcaccacgc cgcgaagggc gacgccgcga acgcggcagc gggcacgcat gaggctgcgg
    12961 gagcctgacg atggcaaccc tgttcccccc acgcagcggc aggctggacg acccggatgt
    13021 gccgctcgac gtcgaattga cccagatgcg ccggcgccac atcccgcagg tgctcgacat
    13081 cgagcggcag gtctacccgc ggccctggac gatgacgctg ttcctgtccg agatcgtgca
    13141 gaggtccacc cgcttctaca tcgtcgcccg cgcgtcccgc cgggtggtcg gctacgccgg
    13201 cctgatgatc ttcggcgacg aggcccacgt caccaacatc gccgtcgacc ccgccgccca
    13261 ccgccgcaag atcgcctccc gcctgctcta cgccctggtc accgaggcca ggtgccgcgg
    13321 cgccaccgcc tgcaccctgg aggtccgggt cgccaaccac ggcgcccagg gcctctacca
    13381 ccagttcggc ttcgccccgg tcgggatccg caagaactac tacgccgaga ccggcgagga
    13441 cgccctgatc atgtgggccg aaggcctcca gacccccgcc tacgagaccc gcctcgccga
    13501 actggcccgc cgcatccccg aaccacgcga ggcccggtaa gcctgtgctg atcctgggga
    13561 ttgagacatc gtgcgacgag accggcgtcg cgctggtcga ggacggcacg gtcgtccgct
    13621 ccaacgtgat cgccgaccag cagacgctgc acgcccgcta cggcggggtg gtgcccgagg
    13681 tcgcctcccg cgcccacgtc gacctgctgc acccggcggt ggagcaggcc tgcgcgctcg
    13741 gcggcgtcca cctcggcgac gtcgacgcga tcgcggtcac catcggcccc gggctggccg
    13801 gcgccctgct ggtcggggtg gccgcggcca agagctacgc gctggcgctg gaccggccgc
    13861 tgctcggcgt caaccacctg atcgggcacg tctacgccaa cgtcctcgac catggcgccc
    13921 tccccctgcc ggcggtggtg ttcgtggtct ccggcgggca caccagcctg ctggtgatgg
    13981 acgagcaggg agcctttcgg gtgctcggcc agaccatcga cgacgccgcc ggcgaggcct
    14041 acgacaaggt cgcgcgggtc ctcggcctcg gctaccccgg cggcccggtg atcgaccggc
    14101 tcgcccgcga gggcgacccg gcggcggtcc gcttcccgcg tgccctgatc gacgacggca
    14161 cctatgattt ctccctctcg ggcctcaaga ccgccgtgct gcgccacgtc cgcgcccacc
    14221 gcgtcgacag cggggagaag ctcaacgtga acgatgtcgc cgcctcgttc caggaggcgg
    14281 tggtggacgt gcaggtcagc aagatcctgc gcgccgccgc cgaagcgggg atcgacaccg
    14341 tgctgctggc cggcggggtc gccgccaact cgcgcctgcg cgcccggttg agtgaggagg
    14401 cggagcggcg cgacgtccgg gtgctggcgc cctcgcccgc cctctgcacc gacaacgggg
    14461 cgatgatcgc ctgcgccggc tggttccagc tcaagcgcgg cgagcgcacc ccgctcgacg
    14521 tcggcgccga cccgaacctc cagatcgtcc ccggcgtcgg ccgcagggcg tcgcggcgtc
    14581 ccaaacggcg tggaggcacc actagcactc ggcatgagtg agtgctaaca tccccgccgg
    14641 aactgtagta ttaaggggta cttgacagcc ccagcagcgg aaacctttcg gcccacccta
    14701 tcgggcgggg ccatcacggc acgaggagga acgatgggcg tcagcctgca gccgctcgag
    14761 gaccggatcg tcgtcaagcc aggtgaaggc gaggagacca ccgcctccgg cctggtcatc
    14821 cccgacaccg ccaaggagaa gccccaggag ggcgaggtcg tcgcggtcgg cccgggccgg
    14881 ttcgaggacg gcaagcgtat cccgctcgac gtcagcgtcg gcgacacggt gatctactcc
    14941 aagtacggcg gtaccgaggt caagtacggc ggcgaggact acctgatcct ctccgcgcgt
    15001 gacgtcctcg ccatcgtcaa gaagtaagtc gggaggacac aagtcacatg gcgaagcagc
    15061 tgctgttcgg cacagacgcg cgacgggcgc tggaggcggg cgtcgacaag ctcgccaacg
    15121 tcgtccgcgt cacgctcggc cccaagggcc gcaacgtggt cctcgacaag aagtggggcg
    15181 cccccacgat caccaacgac ggcgtcacca tcgccaagga gatcgacctc gaggacaact
    15241 acgagaacct cggcgcccag ctcgccaagg aagtcgccac caagaccaac gacgtcgccg
    15301 gtgacggcac caccaccgcc accgtgctcg cccaggccct ggtccacgag gggctgcgca
    15361 acgtcgccgc cggcgccaac ccgatgggcc tgaagcgcgg catcgaacgc gccgtcgagg
    15421 cggtcgccga gcacatccgc aaccaggccc gcgagctgga gacccaggag gagatcgcct
    15481 cggtcgcggc catctccgcc caggacgccg agatcggccg catcatcgcc gaggcgatgg
    15541 acaaggtcgg c
//
LOCUS       DASWDC010000347         3682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1150887, whole genome shotgun sequence.
ACCESSION   DASWDC010000347 DASWDC010000000
VERSION     DASWDC010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150887"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..374
                     /locus_tag="VF995_11100"
     CDS             153..374
                     /locus_tag="VF995_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344147.1"
                     /translation="MAEPESVGQRVLRALNPRRLPTRARTWRAAAAFAALAVVLGALG
                     FWVNHSYLQPATLTAILAVLWGVRAALVR"
     gene            415..1203
                     /locus_tag="VF995_11105"
     CDS             415..1203
                     /locus_tag="VF995_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9344148.1"
                     /translation="MALDLDTGSLLAGLETTHGQLGRLLADLEAASWALPVPATPGWS
                     VGDLVSHVAEGDRAALAALGGTPSFDPASWTGIDDWTAAQVAAHAAERPEERLAAWEA
                     ASDELRGALAGLDEAGWRARVLWVLGPVSARTVAQLRLQEAWLHGHDLAGAAGASWAL
                     DPVTLGWIADIAARVIPGGLSRRGQAKPGAVILLRLDGLGEWRLGGTAGERPAPDATP
                     DLVLEAEPLPFILRAAGRRRDIPWHAAGDEALAAAVAATIFSVG"
     gene            1417..>3682
                     /gene="aceE"
                     /locus_tag="VF995_11110"
     CDS             1417..>3682
                     /gene="aceE"
                     /locus_tag="VF995_11110"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX9344149.1"
                     /translation="MLDQLPDPDPQGTRDWLDSLSDVVEREGPERARFLLRRVLEHAA
                     TLRVGLAPPLSTDYLNTIPASAEPGHPGDEALERRLLSITRWNAAAIVSRANKPELGL
                     GGHMASYASAAELFEVGFNHFFRGKDGDHPGDQVFFQGHSSPGVYARAFLEGRLTERQ
                     LDRFRQEAEPGGLSSYPHPRLMPEFWEFPTVSMGLGAINAIYQARFNQYLARRGLADS
                     AGSHVWAFLGDGEMDEPEAVGALGVAARDELDNLTFVINCNLQRLDGPVRGNGKIIQE
                     LESLFRGAGWNVVKVIWGRRWDELLASDPATAAELVARLNELPDGEWQTLAAESPAYV
                     REHLFGGSERLRALADGRSDEEIIALTVDRGGHDLRKVYAAYHAAVTTKGRPTVVIAQ
                     TIKGRGLGREFEARNSTHQMKKFVRGTLNTFRDRLELTDISDEALADGYPPYYRPAEG
                     SRELRYLLERREALGGPVPRRQVRAQALDLPGPDTYKRLKQGSGKQLVATTMALVRLV
                     KDLLKHPDVGKRIVPIIPDEARTFGMDSLFPELKIYSPKGMTYDAVDRELVLSYKEDA
                     RGQILHEGITEAGSTSEFHAAGTSYATFGLPMIPLYIFYSMFGFQRTADLMWSAADQR
                     ARGFLIGATAGRTTLNGEGLQHQDGHSLLIASTNPACVAYDPSFAFEIPVIVADALRR
                     MYGEDPEDVFYYLTVYNEPFVQPPMPESLDESLILQGLYRYRIAGDGHAHRAQIVTSG
                     SAMPQVLAAADLLAA"
BASE COUNT          529 a         1344 c         1309 g          500 t
ORIGIN      
        1 cctggtcggc ggagaggcta ccttcgaact tgtcggggac gatcaggacg cgcacggcgg
       61 tacgatacct gaccagcctg ccggtgccag cgaaccagca gggacggaac atctcgacga
      121 tcacccggaa cagcgggagc caggaggcgg ggatggcgga gcccgagtcc gtgggccagc
      181 gggtgctgcg ggccctcaac ccgcggcggc tgccgacgcg agcgcggacg tggcgcgcgg
      241 cagcggcgtt cgcagcgctg gcggtggtgc tcggcgcgct ggggttctgg gtgaaccaca
      301 gctatctgca gccggcgacg ctgaccgcaa tcctggcggt gctgtggggt gtgcgcgccg
      361 cgttggttcg ctgactcgcc gtggacgcgg gagagacggc tggaggcgat ggcgatggcg
      421 ctcgatctgg acactggttc gctgctggcg ggcctggaga ccacccatgg acagctgggc
      481 cggctgctgg cggacctgga ggcggcgagc tgggcgctgc cggtcccagc cacgcctggc
      541 tggtcggtcg gtgacctggt gtcgcatgtg gccgagggcg accgggcggc gctggcggcg
      601 ctcggcggga cgccctcctt cgacccggcg tcctggacgg ggatcgacga ctggaccgcc
      661 gcgcaggtgg cggcacacgc cgccgagcgg cccgaggagc ggctggccgc ctgggaggcc
      721 gcctccgacg agctgcgcgg cgccctcgcc gggctggacg aggccggctg gcgggcgcgg
      781 gtgctgtggg tgctcggccc ggtgtcggcg cggacggtgg cgcagctgcg cctccaggag
      841 gcctggctgc acggccacga cctcgccggg gcggccgggg cgtcctgggc gctcgacccg
      901 gtgacgcttg gctggatagc cgacatcgcg gctcgggtga tccccggcgg gctgagccgc
      961 cgcggccagg caaagccggg cgcggtgatc ctgctgcgcc tggacggcct cggcgagtgg
     1021 cgcctcggcg gcaccgccgg cgagcgcccc gcgccggacg ccactccgga cctggtgctt
     1081 gaggccgagc cgctgccgtt catcctgcga gcggcgggcc gccgccggga catcccctgg
     1141 cacgccgcgg gagacgaggc gctggccgcc gccgtggcgg cgacgatctt ctctgttggc
     1201 tagttcgtag ttttcgccgc gaatgcgctg aattgcaatc ccgcgcaatc ctcaagggtc
     1261 tggaacactt gggaaccgcg cgtgggggtc tcgccgcgag cgacaacggc gactaaggtg
     1321 ataggtgaaa ctgatcatgg ccattggcaa acccgatgcc cgaacgagcc cgggagcagc
     1381 acggtgagcg accacgacga ggtcaccacg ggcgggatgc tcgatcagct tcccgacccg
     1441 gatccccagg gaaccaggga ctggctcgac tccctcagcg acgtcgtcga gcgggaaggc
     1501 ccggaacggg cccgcttcct gctacggcgg gtgctggagc acgcggccac cctgcgggtg
     1561 gggctggcgc ccccgctctc caccgactac ctgaacacca tccccgccag cgccgagccg
     1621 ggccaccccg gcgacgaggc gctggagcgg cgcctgctgt cgatcacccg ctggaacgcc
     1681 gccgcgatcg tctcgcgggc caacaagccg gagctgggcc tcggcggcca catggcctcc
     1741 tacgcctcgg cggcggagct gttcgaggtg ggcttcaacc acttcttccg gggcaaggac
     1801 ggcgaccacc ccggtgacca ggtcttcttc cagggccact cctcccccgg cgtgtacgcg
     1861 cgggccttcc tggagggccg cctgaccgag cggcagctcg accgcttccg ccaggaagcc
     1921 gagcccggcg ggctgtcgtc ctacccgcat ccccggctga tgccggagtt ctgggagttc
     1981 ccgacggtct ccatgggcct tggtgcgatc aacgcgatct accaggcccg cttcaaccag
     2041 tacctggcgc ggcgcgggtt ggccgacagc gccgggtcgc acgtatgggc gttcctcggc
     2101 gacggcgaga tggacgagcc ggaggcggtc ggcgccctcg gcgtggccgc ccgcgacgag
     2161 ctggacaacc tcaccttcgt gatcaactgc aacctgcagc gcctcgacgg gccggtgcgt
     2221 ggcaacggca agatcatcca ggagctggag tcgctgttcc gcggcgccgg ctggaacgtc
     2281 gtcaaggtga tctggggccg ccgctgggac gagctgctgg ccagcgaccc ggccaccgcc
     2341 gccgagctgg tcgccaggct gaacgagctg cccgacggcg agtggcagac cctggccgcc
     2401 gagagtcccg cctacgtccg cgagcacctc ttcggcgggt cggagcggct ccgcgccctc
     2461 gccgacggcc gcagcgacga ggagatcatc gcgctgaccg tcgaccgggg cggccacgac
     2521 ctgcgcaagg tctacgcggc ctaccacgcg gccgtcacca ccaagggccg cccgaccgtg
     2581 gtgatcgccc agacgatcaa gggccgcggc ctcggccggg agttcgaggc ccgcaacagt
     2641 acccaccaga tgaagaagtt cgtgcgcggc accctcaaca ccttccgcga ccggctggag
     2701 ctcaccgaca tctccgacga ggcgctcgcc gacggctacc cgccgtacta ccgccccgcc
     2761 gagggctccc gggagctgcg ctacctgctg gagcgccgcg aggcgctggg cggcccggtc
     2821 ccccgccgcc aggtccgcgc ccaagccctc gacctgcccg gccccgacac ctacaagcgc
     2881 ctcaagcagg gctccggcaa gcagctggtg gccaccacga tggcgctggt ccggctggtc
     2941 aaggacctgc tcaagcaccc ggacgtcggc aagcggatcg tgccgatcat ccccgacgag
     3001 gcgcgcacct tcgggatgga ctcgctgttc ccggagctga agatctactc gccgaagggg
     3061 atgacctacg acgcggtcga ccgcgagctg gtgctctcct acaaggagga cgcccgcggc
     3121 cagatcctgc acgagggcat caccgaggcc ggctcgacga gcgagttcca cgccgccggg
     3181 accagctacg cgaccttcgg cctgccgatg atcccgctgt acatcttcta ttcgatgttc
     3241 gggttccagc gcaccgccga cctgatgtgg agcgccgccg accagcgcgc ccgcggcttc
     3301 ctgatcggcg ccaccgccgg ccgcaccacc ctcaacggcg agggcctgca gcaccaggac
     3361 ggccactcgc tgctgatcgc ctccaccaat ccggcctgcg tcgcctacga cccgtcgttc
     3421 gccttcgaga tcccggtgat cgtcgccgac gccctgcgcc gcatgtacgg cgaggacccc
     3481 gaggacgtct tctactacct gacggtctac aacgagccgt tcgtgcagcc cccgatgccc
     3541 gagagcctcg acgagtcgct gatcctccag ggcctctacc gctaccgcat cgccggggac
     3601 ggccacgccc accgcgccca gatcgtcacc tccggcagcg ccatgcccca ggtgcttgcg
     3661 gccgccgacc tgctcgccgc cg
//
LOCUS       DASWDC010000348         4327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1152016, whole genome shotgun sequence.
ACCESSION   DASWDC010000348 DASWDC010000000
VERSION     DASWDC010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152016"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VF995_11115"
     CDS             <1..752
                     /locus_tag="VF995_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX9344150.1"
                     /translation="TRGARGLAWVAVEEHGLRSPLDKFFAEGERAELRRVTGAQPGDL
                     VLIAADRTRLAQTILGELRTRLAAERGLIPADRWAFAWITDFPMFEWNETEGRWDAAH
                     HPFTSPSPKWEERFDDAKDEATARAYDLVLNGYELGSGSIRIHQADLQRRVFAVLNIT
                     PAEAEEKFGFLLRGLSFGAPPHGGFAFGLDRLYMLLAGEHSIRDVIAFPKTQSGSDPL
                     TGAPTEVPADQLAEVRLRTLPPPPAKPNPAD"
     gene            complement(765..1808)
                     /locus_tag="VF995_11120"
     CDS             complement(765..1808)
                     /locus_tag="VF995_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX9344151.1"
                     /translation="MKIQILCTLGPASMSEDVIRQLDRAGVDLFRINLSHTRFETLDG
                     AIDLVRRATGKPLCLDLEGAQVRTGPVARGLALEEGQPVQLVAAEVDGTAATLTLRPA
                     SVFTSLAPGHRIAIDFDGAQVEVTRVRVDSADPASGMAEAVVTSGGRVGANRAVTVEP
                     SPELPTLSVKDQQAIVLGLRRGVEQVALSFASCADDVALVRSLLTEGTGLIAKIESSA
                     GVRNLDGIIAAADAVLIDRGDLSREVPLEHVPYYQKAIVRRANRWHRPVFVATNLLES
                     MVSNRNPTIAEANDIANTLLDGVHGLVLAAETAIGRDPVGIVGMVSRSIQAFEQSSFA
                     NLLERDQPLLTTR"
     gene            1913..2968
                     /locus_tag="VF995_11125"
     CDS             1913..2968
                     /locus_tag="VF995_11125"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9344152.1"
                     /translation="MTCPQHGQCCRAEAVRCGGLRCGGFATVAPDALVAVGFLEVIEL
                     LFFVFGTGRCGSTPVIEVLARHRDVGFVSNVDDKFSYLNLNGRWNNTLFRRSAPRDPG
                     LLPMAGRSRLFELGRLRISPSEGWEVLERQVSPMFSHTCRDLVAGDATPWMAKRVQGF
                     FERRMAAQHKPVFGHHLTGWPRAGLLRAVFPEARFIHVVRDGRAVANSWLHMPWWSGY
                     KGPDNWYLGPLPPHYQRDWEASGRSFVVLAGLAWKLLLDAFADARAATPPGQWLEVRF
                     EDILTEPRKQFEAMLEFLNLDWTPAVEAGFTRYQFQQSRGDAFRRDLDPANLALLERY
                     IGDRLLAYGYDLSARAD"
     gene            complement(3274..3690)
                     /locus_tag="VF995_11130"
     CDS             complement(3274..3690)
                     /locus_tag="VF995_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019544961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO5389 family protein"
                     /protein_id="HEX9344153.1"
                     /translation="MSLEVTPALLAQAEQGDVDHAEFVACVRDSLPYAWDTISRLAAE
                     LQRTGADEPTAFADNQLPPPSEPARGQLLRALASDAIRGALERHFGVTLAFQNCHRVA
                     VFPAGARSGGDSADRYREFTSVRAQLLNQSPALRDC"
     gene            complement(3735..4327)
                     /locus_tag="VF995_11135"
                     /pseudo
     CDS             complement(3735..4327)
                     /locus_tag="VF995_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895630.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          615 a         1578 c         1483 g          651 t
ORIGIN      
        1 agacccgcgg cgccaggggc ctcgcctggg tcgcggtgga ggagcacggc ctgcggtccc
       61 ccctcgacaa gttcttcgcc gagggcgaac gcgccgagct gcgccgcgtc accggcgccc
      121 agcccggcga cctggtcctg atcgccgccg accgcacccg cctcgcccag accatcctcg
      181 gcgagctgcg cacccgcctg gccgccgaac ggggcctgat ccccgccgac cgctgggcgt
      241 tcgcctggat caccgacttc cccatgttcg aatggaacga gaccgagggc cgctgggacg
      301 ccgcccacca ccccttcacc tcgcccagcc ccaaatggga ggagcgcttc gacgacgcca
      361 aggacgaggc caccgcccgc gcctacgacc tggtcctcaa cggctacgag ctgggcagcg
      421 gcagcatccg tatccaccag gccgacctgc agcgccgcgt cttcgcggtc ctgaacatca
      481 cccccgccga agccgaggag aaattcggct tcctgctccg cggcctctcc ttcggcgcgc
      541 ccccacacgg cggcttcgcc ttcggcctcg accgcctcta catgctcctg gccggcgagc
      601 actccatccg cgacgtgatc gccttcccga agacgcaatc cggctcagac cccctcaccg
      661 gcgcccccac cgaggtcccc gccgaccagc tcgctgaggt ccgcctccgc accctgcccc
      721 caccgccagc aaagccgaac cctgcggact gaagggcgct ggcgtcagcg ggtggtgagc
      781 agcggctggt cgcgttccag caggttggcg aagctggact gctcgaacgc ctggatcgag
      841 cggctgacca tgccgacgat cccgaccggg tcgcggccga tcgcggtctc ggcggccagg
      901 accaggccgt ggacgccgtc gagcagggtg ttggcgatgt cgttggcctc ggcgatggtc
      961 ggattgcggt tgctgaccat cgactccagc aggttggtcg ccacgaacac cgggcggtgc
     1021 cagcggttcg cgcggcgcac gatcgccttc tggtagtagg ggacgtgctc cagcgggacc
     1081 tcgcgggaga ggtcgccccg gtcgatcagg accgcgtcgg cggccgcgat gatcccgtcc
     1141 aggttgcgga ccccggcgct cgactcgatc ttggcgatca gcccggtccc ctcggtgagc
     1201 agcgagcgga ccagcgcgac gtcgtcggcg cagctggcga acgacagcgc cacctgctcc
     1261 acgccccggc gcaggccgag cacgatcgcc tgctggtcct tgacgctcaa ggtcggcagc
     1321 tccggcgacg gctcgacggt gaccgcccgg ttcgctccga cccgcccgcc gctggtcacc
     1381 accgcctcgg ccatgccgct cgccgggtcg gcgctgtcca cccggacccg ggtcacctcg
     1441 acctgggcgc cgtcgaagtc gatcgcgatt cggtgacccg gcgccagcga ggtgaacacg
     1501 ctggcgggcc gcagggtcag cgtggcggcg gtcccgtcga cctcggcggc gacgagctgg
     1561 accggctggc cctcctccag cgccaggccg cgcgcgaccg gccctgtgcg cacctgggcc
     1621 ccctccaggt ccaggcagag gggcttcccg gtggcgcggc ggaccaggtc gatggcaccg
     1681 tcgagggtct cgaagcgggt gtgggacagg ttgatccgga acaggtccac cccggcacgg
     1741 tccagctgcc ggatgacgtc ctccgacatc gacgccggac cgagcgtgca caggatctgg
     1801 atcttcaccg accgtccgcc cccaattcag gccaccgcca aaccagctca aggcgctgtt
     1861 catcagcgcc gctgccacca tgccgggtcc gcaccagcgg gcgtacctgc gaatgacgtg
     1921 cccgcaacac ggtcaatgct gtagggcaga ggcggtacgc tgcggagggc ttcgctgcgg
     1981 aggcttcgcc accgtcgccc cggacgccct ggtcgccgtc gggttcctgg aggtcatcga
     2041 attgctgttc ttcgtgttcg gcaccggccg ctgcgggtcg acgccggtga tcgaggtgct
     2101 cgcgcggcac cgggacgtcg gcttcgtctc caacgtcgac gacaagttca gctacctcaa
     2161 cctcaacggc cgctggaaca acacgctgtt tcgccgctcg gccccgcgcg acccggggct
     2221 gctgccgatg gccggccgct cccgcctctt cgagctcggc cgcctgcgga tctccccgtc
     2281 ggagggctgg gaggtgctgg aacggcaggt ctcgccgatg ttcagccata cgtgcagaga
     2341 tctcgtcgcc ggcgacgcga ccccctggat ggccaaacgc gtccagggct tcttcgagcg
     2401 ccgcatggcc gcccagcaca agccggtctt cggccaccat ctcaccggct ggcctcgcgc
     2461 cgggctgctg cgggcggtct tccccgaggc gcgcttcatc cacgtggtcc gcgacggccg
     2521 cgcggtggcc aactcctggc tgcacatgcc ctggtggagc gggtacaagg gcccggacaa
     2581 ctggtatctc ggtccgctcc ccccgcacta ccagcgtgac tgggaggcgt cgggccgctc
     2641 cttcgtggtg ctcgccggcc tggcctggaa gctgctgctg gacgccttcg ccgacgcccg
     2701 cgccgccacg ccgcccggcc agtggctgga ggtccgcttc gaggacatcc tcaccgaacc
     2761 gcgcaagcag ttcgaggcga tgctggagtt cctcaacctc gactggaccc ccgccgtcga
     2821 ggccggcttc acgcgctacc agttccagca gagccgcggc gacgccttcc gccgcgacct
     2881 cgaccccgcc aacctcgccc tgctggagcg ctatatcggc gaccgcctcc tggcctatgg
     2941 atatgatctt tccgctagag cggattagcc ggaatcatag gtagaacagg ctagtcacag
     3001 gcttgctcag taccgacgag cgtggaacaa tcggcctctc ggcagcgccc gacgttgttt
     3061 gtggccggct attcgtttgc gccaacactt cgacgtaggg gctcgacctc caggtcgggg
     3121 aaggcacccg gaacgatcgg gtaccggaaa cgaccggggc ggaccccaac ctgttccgta
     3181 gagagcaggt gcaaacgtcg accggccacg gcacccgtgg agcgctgccg acacccttct
     3241 ccgcccctgt tggggtccgc tgcgtttcag cgctcagcag tcgcgcagcg ctggcgactg
     3301 gttgaggagt tgcgccctga ccgaggtgaa ctcacggtag cggtcggcgc tgtcgccgcc
     3361 gctgcgggcg ccggccggga acaccgcgac ccggtggcag ttttggaacg ccagggtgac
     3421 gccgaagtgt cgttccagcg cgccgcggat cgcgtcgctg gccagagcgc gcaggagctg
     3481 gccgcgggcc ggttcgctgg ggggtggtag ctggttgtcg gcgaacgcgg tgggctcgtc
     3541 ggcgccagtg cgctgcagct cggccgccag gcggctgatg gtgtcccagg catacggcaa
     3601 cgagtcgcgc acacaggcga cgaattcggc gtggtcgaca tcgccctgct cggcctgggc
     3661 cagcagcgcc ggcgtgacct ccagtgacat gctcatgctc cttttcgggt tcggtcaggt
     3721 ggggtgacgg gtggtcaggg ggtggtggcg agccgcagcg cgtccggccc gagggtgaac
     3781 tcgggatcga cctgggtggc gaggtcctcg ccggtgcggg cattggccca ggcgcgggcg
     3841 ttgcggaggt ggaagtggac tgcctggcgg ctgaacctcg cccagtcctt ggagacggca
     3901 tcgaccgccc cgcggagccg ctccagcgcg gtgcggttgg ccacctccag cgcctccagt
     3961 gcaagcgggc ggccctgctg ctgctcggcg gcctgcaccg cgctggagtg ctccacgcag
     4021 accagcaggt cggcgccgag ctgctcccgc aggaacgcgg cgtcggcttg ggtctgcacc
     4081 ttgttgccga ccgccgagag ggtgacgtcg agccgcgggc gtagtcctgg tactggcgcc
     4141 agaccgccac cgacttgcgc gtcggctcga ccaccaggaa ggtgcggtcg aagcgagtga
     4201 acagcccgga ggcaaagcag tcggccccgg cggtcaggtc gacgaccacg tactcgcccg
     4261 ccccgtcgac caggtggttg agcagcagtt cgaccgcgcc ggtcttggcg tggtagcagg
     4321 ccacgcc
//
LOCUS       DASWDC010000349        19548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1569249, whole genome shotgun sequence.
ACCESSION   DASWDC010000349 DASWDC010000000
VERSION     DASWDC010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19548
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569249"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VF995_11140"
     CDS             <1..813
                     /locus_tag="VF995_11140"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075036.1"
                     /note="AcsA; in Sinorhizobium meliloti this enzyme is
                     required for acetoacetate activation; similar to
                     acetyl-CoA synthase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEX9344154.1"
                     /translation="SPLPPEGFDWLYEHVSGDVLVGSVSGGTDVCTAFVGSCPTLPVY
                     AGEIQCAMLGAKVEAFDEQGRSVVGQVGELVLTEPLPSMPLYFWNDPDGVRYRDSYFS
                     TWPGVWRHGDWVEVTERGSLIITGRSDSTLNRGGVRMGTSEFYRVVEGLDEIIDSLVV
                     DVSEPGGDGRVLLFVVLRPGAELDAALAKAIRAAVRQELSPRHAPDEIHAIAAVPRTL
                     SGKKLEVPVKRILTGVPLERAVSEGALANPEAIHEVVALAGGAPPNGPDTEN"
     gene            996..1607
                     /locus_tag="VF995_11145"
     CDS             996..1607
                     /locus_tag="VF995_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX9344155.1"
                     /translation="MTNRRRGVTFAATLLGLVAAATAFSAPAFARHSDVQLTSSCDRS
                     DDRLRVDYTVRASEPGHAGSADLSYAVAGGASHPLGRGTFTAKRDTISGHFLLHVPPN
                     QDKPLTLTAVTHWPDRDDATNSATVRLTHCRKRTPGTTTSQPPVTSQVPRTSIQTVAV
                     REALPYTGSNSGPLLLAAVSLLGAGTAVLVASRSRRQRGRIAR"
     gene            complement(1686..3455)
                     /locus_tag="VF995_11150"
     CDS             complement(1686..3455)
                     /locus_tag="VF995_11150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018583969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9344156.1"
                     /translation="MADGALIVQSDRTVLLEVDHPKADAARVAIAPFAELEKSPEHVH
                     TYRLTPLALWNARAAGIEAAQVRAALTEHSRYPVPPALLADLLETMDRYGRLTLRGDP
                     RNSGQLVLEAAEEAVLAEVVRAKQVAPLVGERLGPTRVLVPLGERGRLKQALLQLGWP
                     ADDQAGYVEGTPLPQAWREADARSGERFALRRYQREAVTAFAAAGSGVIVLPCGAGKT
                     LVGLGAMAAVQARTLILVTSTLAARQWRDELLARTTLEPSQVGEYSGARKDVAPVTIA
                     TYQVLTTRRRLSTQASEAAPSTGDRATGDRRRRSNGDYQHLDLLGLQDWGLVVYDEVH
                     LLPAPVFRMTAEIQARRRLGLTATLIREDGREGDVFSLIGPKRYDVPWRDLEVQGWVA
                     AADCVEVRVTLTDDERMGYATAEPEERYRRAATARSKLPVVRRLAELHRDDRVLVIGQ
                     YLDQLETLGKELDAPVITGQTPNTERERLFAQFRSGELARVVVSKVANFSLDLPEANV
                     AIQVSGTFGSRQEEAQRLGRILRPKAGGGGARFYTVVSRDTNDTEYAQRRQRFLAEQG
                     YAYRLVDAAAVLNGGSPLPGLET"
     gene            complement(3448..5898)
                     /locus_tag="VF995_11155"
     CDS             complement(3448..5898)
                     /locus_tag="VF995_11155"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEX9344157.1"
                     /translation="MASLSDAQLGCLLRSRPDLLTPASHAGGREGSRPWGAGGAAEHA
                     GGRSEAVHGGPAEAKAEPPPASFAELASRAGSPASLGAALRSLDLAALQLAELLALVG
                     LPTTVEALAAAAGPGLSPERLHQGLAALSKLGLALPAEDGSIAGPRGLHAPFGNPGRL
                     GPSVAELAKVGVAREQLDRIAAHLGLLIDRPARKVDLVAAVGAALADRALVARLLAEA
                     GEPARALLDQALTAPGPLTILGVGDGRFARNPDAEPATWLLDHGLLLPTTYSRFVVPR
                     EAAIGLRGGLVFPEWPAAPTPRLLDPVPDAPDRARAAALRLVVAAEGVLTRLDRDPLP
                     LTQAGTVAVRDLRRLARELSLADDELAFLIDLLAEAELLAVGGPWDQRTLALRPEADA
                     WLAGSRACRWADLAVAWRNRDLAFEDHLLPRHEPPVPGAERARPLGGHRLAATRDRRR
                     SLLAALTRLPAPAEARVPRPVDNQNAPPSELDRLNSGGPPTGPGVATAGPGIAAVDTD
                     DLAALLAWQEPLTWPGFAPEHTLVGAAFAARAVAVRTPPQRLIATVLAVADLAELLGL
                     AVRADGPAGRIAAGPAAAAWAAGAGAEELAEQLQVDGALPQGSTRFLVAGDLTVVAPD
                     GLAPHVESRLRYLADREPGSGSVWRLHEPSLRRAFDHGLGADDVLGFLREHSSTPLPQ
                     ALEYLVDDAARRHGRLRVGDAATYLRGDPAQVAQVVRSAAGRKLGLRELAPGVAVTVA
                     TQRQLIAVLRKLGEAPLAEEPDGTARLESPKAVRHARPLPAQLRPHPAPRAAAGAADP
                     AELVARLRSSGEEAAHGG"
     gene            complement(6147..6962)
                     /locus_tag="VF995_11160"
     CDS             complement(6147..6962)
                     /locus_tag="VF995_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9344158.1"
                     /translation="MERRALGSSGLKVPVIGMGTWRTFDVRGVAAESTCRRIVAEALA
                     AAANFFDSSPMYGQAERVLGRGLEGRRAEALVATKVWTPDDEEAERQVERSLQYAHGH
                     LELLQIHNLVAWPARLDLLERVRDDGLVTAIGATHYDSHAFDELITVMRSGRISAVQI
                     PYNPLEREVERTILPLAADLDLGVVLMRPFARGAVLSRWPKPSELAPLEPFGVRTWPQ
                     ALLKWGLSDPRCTVAIPATSRKGRMTENAAAGDPPWYGPDERDFVTRLATRTL"
     gene            complement(7075..7902)
                     /locus_tag="VF995_11165"
     CDS             complement(7075..7902)
                     /locus_tag="VF995_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9344159.1"
                     /translation="MTAATAPAPASERTLATPSGFASDVLALTTRSIRALLREPESFI
                     PALAIPLFFFVVQIGALGKIAGPAFGIKNYAAFQLPVAVLFASANTAGGNSLVLDIDG
                     GYWDKLSLTPVRRPAMVIGTLLGEMFSVVSYSVLLTLLGLAMGVRYAGGELGGALGLL
                     ALTVLFGVGFASVSVAVALATGSVRATQTTFVIFFPLLFLTPGTLPRSLMQGWFKAAV
                     SVNPITYVLEAGRAFIQETGVAFNVATVSKGFIAALAFCALTFSLTALAFRRRARMG"
     gene            complement(7899..8996)
                     /locus_tag="VF995_11170"
     CDS             complement(7899..8996)
                     /locus_tag="VF995_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9344160.1"
                     /translation="MATAIQATKLVRRFEEVEAVRGVSLEVEEGEIFGFLGPNGAGKS
                     TTVRMLTTLLEPTSGTGSVLGHDVVQEAATVRGLIGVALQEAGLDPKQTGRELLTLQA
                     RLYGLRGAVLAEQIHEVTELVGLSDAIDRRIKGYSGGMKRRLDLASALLHRPRVLFLD
                     EPTTGLDPTSRTLIWDRVRELRQRYQTTVFLTTQYLEEADQLADQVAIIDRGQIVRQG
                     TPAALKAEVGADVIDVAVDDRECGAARVTILEMQERGELPAGELRGTASGYTLFVPDG
                     PSNVAVLLRGLDRAGITTGPISVSRPTLDDVFLAATGGRMASRAAEESSPDGGDQRRD
                     RDSDGDRPGATTGAGTGAGTGAGTGTGRGSA"
     gene            9134..9838
                     /locus_tag="VF995_11175"
     CDS             9134..9838
                     /locus_tag="VF995_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX9344161.1"
                     /translation="MRIQLDALAASIGGLGELDVERGFAPALQRVVDAAKKLFGAGGA
                     GLMLVDADGVLRWASASDQAAQTVEDSQERLAQGPCLEAFEQHRVAYLRDVHAEPRWR
                     ELAQVMAAERMVAALSVPVELDGGLIGTLDVYFDIPWEWDPSEVSALRAYAAVVASLL
                     QHAMVAHVKGRLADQLQVALDHRSLIEQAKGVLMEREGLDQTAAFERLRRTARSQRRR
                     IVELAREVVDKTQLPS"
     gene            complement(9889..10929)
                     /locus_tag="VF995_11180"
     CDS             complement(9889..10929)
                     /locus_tag="VF995_11180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9344162.1"
                     /translation="MPTGPATFGIWTELRPGGGRQPDPAYYRERLDEAVLAEQLGFTA
                     VWGSEHHAVEDAHLSQQLPFLAAVAARTERVRVGTGVLLLPLYRPRDVAEQAAVVDLV
                     SGGRLILGFGGGYVEREFDAFAVDRGQRGRLLEEKVAWLRRAFAEGVAADGPDGTDLP
                     VGPRPVQLSGPPLYLGGGAERALDRVARIGDGWFALAHFRWQKTAERYPVLRAELERA
                     GRPAEGFPIVVGVHLRVSDDPERTWATELAPAIADQLDRYGDWSTDRGKPRPAPIDAA
                     RLKRSAVFCDTPDGVVAALREFQAQLPFTHVALWSRPAGISHEAACANLERIAREVAP
                     AFAGSGEAPAWR"
     gene            complement(10970..11695)
                     /locus_tag="VF995_11185"
     CDS             complement(10970..11695)
                     /locus_tag="VF995_11185"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX9344163.1"
                     /translation="MPDHVIPHPDLAGYVLGALDPGEVGAFEAHLAACPECRQELAEL
                     EGLPAMLERATPPVELPPGLRERTFAAVDRAAAEAESEAAAQAGARTARPGARRSLRW
                     LAVAAAAVVILVGGLATGIRLFGPGPGTNFALAAPNGGPASGHARVHRTRAGLVVDLT
                     VKHLPPADAGHVYECWFVGPGDTLQNQNRISAGTFTVGPDGSASVHMVTAADLARFPK
                     MGITLEPNDGIPLRRGPKLLASP"
     gene            complement(11688..12269)
                     /locus_tag="VF995_11190"
     CDS             complement(11688..12269)
                     /locus_tag="VF995_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9344164.1"
                     /translation="MSERMLDAAGQTAADEADLLASVAAGDRDAPLEELYRRYGPRLY
                     GLGLRLLGDQGLAEELVQEAFVRLWQQAGRFDPARGSVSAFLFTIARRIAVDLWRRPS
                     SRPFEPPPPEAIAGEQGHDQVERLIEGLAVRDALQSLSPAHQQVLELFIGQDRKQAEI
                     ATLLGLPLGTVKTRAYYALRALKLALQERGINA"
     gene            12567..13055
                     /locus_tag="VF995_11195"
     CDS             12567..13055
                     /locus_tag="VF995_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX9344165.1"
                     /translation="MLVAVSLLSLTGMLAAGCASKSGSGSGSGSGGLYGNSNAPTTTA
                     AASGSGSTASSSGSSSGGRYGSGSGSGNTGTASATMTGTKVTINNFAFGPVALKAKIG
                     QKVTWTDAQMGVRHTVTANSGAFNHLLTPGTSFSFTFMKAGTYAYHCNIHPTMHGTVV
                     VT"
     gene            complement(13182..14381)
                     /locus_tag="VF995_11200"
     CDS             complement(13182..14381)
                     /locus_tag="VF995_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344166.1"
                     /translation="MRTVKVLAAGALVALALAACSSGKGSGGPASGGGSGAAKGADLR
                     IQFDKQLGEHAILSVAMLQDMINRQPAAAPEAALLVGRNTDDLGASFGSAFGTSAGNQ
                     FKEQLKNQVGILVRYTVAKGGGDQPGAAKALKDLGAYQAQFVTFMAATAPKLPKQAVA
                     SDLKQQFAGMVAAVDASVRHDQAQAASQFAAVYEHAFTTGSALAAVGGDAAPRTGALS
                     AKPDDLRTTLDMQRGEQAALTSLVLGKAISGAPDGKAFGAELGKNTQALTQSVASVYG
                     RAAGTKFKTLWNDHLSALLNYTAAKATGDQGKVKKSQAALASFSDSLAGFLAGANPSL
                     SKSELAGLLTKQVSQAAGAVDATYAKSTIVATSAMAGAYEQSFTIGDTLAAAIAKQFP
                     DKFAPAN"
     gene            complement(14609..15265)
                     /locus_tag="VF995_11205"
     CDS             complement(14609..15265)
                     /locus_tag="VF995_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX9344167.1"
                     /translation="MTAGVRLTPEAIRDKTFTRARQGYRPGEVDQFLAQAADDLARLA
                     ECYASGSRTPAGLLGADEVETKLFHRAFRGYAMAEVDDFLDLVAADLGRMHRLVAEMV
                     RVAQPQLELTPRVEPLPDRMTASDVASTTFTRIVRGYRMDEVDRFITLVARELARVED
                     NPDSAPRITAHDIGVTRFTLGPRGYAMYEVDSFLARLAAQLARAEAENGYPYDFPRYT
                     "
     gene            15417..17159
                     /locus_tag="VF995_11210"
     CDS             15417..17159
                     /locus_tag="VF995_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9344168.1"
                     /translation="MSTRPARAEAAPPAEQLDPRRWLALGVVLIAAFMLLIDISIVNV
                     AIPSIQRDLHASYSQVQWVLAGYQLAFAVVLITGGRLGDIYGRKRLFLIGVTGFTAAS
                     ALCGAAQNPAMLVGSRFLQGLMGALLFPQVLSIIQVEFAPRERGTALGMFGAAIGLAT
                     ISGPLAGGLLIQGNLLGLDWRPIFLVNLPIGVIAVVAAVVLLRESRAEDAPRLDPVGV
                     VLATAALLALVYPLVEGRDLGWPVWIFVLLAASVPLFAIFAAYERLKTRRDNSPLLVL
                     DLFRERAFVSGLLLSAVFFSGIAAFFLTFSLFLQIGLGFTALHAGLTTIPFSFGTAVA
                     SAASTRLAPRLGRRVLSLGCLLLAAGMGGVILTVSHYGTALHSWQLLPALLLCGIGLG
                     LVVAPLVNVILAGISGRNAGSASGALTTIQQVGGAIGVAIIGVIFFGLLSSEANPTVD
                     QVAPQLRSGLETAGLPAPASDQVVAGFRVCFRDRASAKDPTSTPASCQRLQGQSQNQG
                     QSGSGGGQAQGQQAGQAIQAGATAARKQDFVSSVERTLWYEVAVYGLSFLLVFLLPFV
                     PGHRLRPGGGPAAA"
     gene            complement(17183..18097)
                     /locus_tag="VF995_11215"
     CDS             complement(17183..18097)
                     /locus_tag="VF995_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9344169.1"
                     /translation="MTHDHQDHLKSPASAAGAHRGRLLAVLAITLAILVAEVVGGVLA
                     GSLALLADAGHVAADAAGIGMSLLAIAFAARPATPARTYGHFRLEILAAVVNGVLLFG
                     VAALVLVEAVRRLLAPPEVASGVMAAFGMVALAGNAGSLLLLRRAQGEALPLRGAFLE
                     VLGDLLGAVAVVVAAVVIAATGWERADAAASALVGLLILPRTWRLLRDAVDVLLEATP
                     KGVDLADVRQHILSAPGVADVHDLHVWTITSGMNVVSAHVVLAGGATAGPVLDHLCEC
                     LGGHFDIEHSTFQLEQPEHRGHETPLHQ"
     gene            complement(18152..19297)
                     /locus_tag="VF995_11220"
     CDS             complement(18152..19297)
                     /locus_tag="VF995_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9344170.1"
                     /translation="MNDLLTERERDVRERVRTFCDKEVTPIINDYWERAEFPFELVPK
                     LAELGVAGGTIEGDGCPGLSPVATGLIAMELARADGSVCTFFGVHSGLAMQSIALLGS
                     EEQKRRWLPAMARMDKIGAFALTEPEHGSDAVALETRARRDGDCYVLDGAKRWIGNAS
                     FADLVIVWARDEAGKVGGFVVEKGTPGFNASVMTGKIAKRAVWQAEITLEGVRVPVAN
                     RLAGANGFGDTAKVLAATRFGVAWEAVGHAMAAYESALSHAKQREQFGAPIASYQLVQ
                     HKLATMLAEITAMQLICLRLSQLAGSGQMTDGMSSLAKMHNAAKARRVVADARDILGG
                     IGILLENHVARHQADMEAVFTYEGTDSIQSLIVGREITGVSAFTRKA"
BASE COUNT         2679 a         6816 c         7324 g         2729 t
ORIGIN      
        1 tcgccgctgc cgcccgaggg gttcgactgg ctgtatgagc acgtcagcgg cgacgtgctg
       61 gtcgggtcgg tcagcggcgg gaccgacgtg tgcaccgcct ttgtcgggtc ctgcccgacc
      121 ctgccggtct atgccggcga aatccagtgc gccatgctcg gcgccaaggt ggaggcgttc
      181 gacgagcagg gccgctcggt cgtcgggcag gtcggcgagc tggtgctcac cgagccgctg
      241 ccgtcgatgc cgctgtattt ctggaacgac cccgacggtg tgcgctaccg cgacagttac
      301 ttctccacgt ggccgggcgt gtggcggcac ggggactggg tcgaggtcac cgagcgcggc
      361 tcgctgatca tcaccgggcg ctcggactcg accctcaacc gcggtggcgt gcgcatgggg
      421 accagcgagt tctaccgcgt ggtcgagggc ctcgacgaga tcatcgacag cctggtggtc
      481 gacgtcagcg agccgggcgg cgacggccgg gtgctgctgt tcgtggtgct gcgcccaggc
      541 gccgaactgg acgccgcgct ggccaaggcg atccgcgccg ccgtgcgcca ggagctgtcg
      601 ccgcggcacg cccctgacga gatccacgcg atcgccgccg tgccgcggac gctcagcggc
      661 aagaagctcg aggtgccggt caagcggatc ctcaccggcg tccccctgga acgcgcggtc
      721 agcgagggcg ccctcgccaa cccggaggcc atccacgagg tggtggccct ggccggcgga
      781 gcgcctccca acgggccaga cacggagaac tagacactct cctcgtagtt ctcatctgct
      841 ccaatctgcc gctagttatc aaactgagac tccagcgtgc cggacttttc cttcgattcc
      901 ctcgaatcgg accaatacct ccgatacatt gcccaggccc tcagttcagg acgtaggccg
      961 tagagcaggt ggatcaacaa ggagcgagat cgttaatgac gaaccgacgc cgcggcgtga
     1021 cgttcgcggc aacactgctg ggtcttgtgg ccgcggcgac ggcgttcagc gcgccggcgt
     1081 tcgcccggca cagcgacgtg cagctgactt cctcgtgtga ccgcagtgac gaccggctgc
     1141 gggtcgacta caccgtgcgg gcgtcggagc cgggccacgc cggcagcgcc gacctctcat
     1201 atgccgttgc cgggggggcg tcccacccgc tggggcgggg aaccttcacg gccaagcgcg
     1261 acaccatcag cgggcacttc ctgctccatg tcccgccgaa ccaggacaag ccgctgacgc
     1321 tcaccgcggt gacccactgg ccggaccgcg acgacgcgac caacagcgcc accgtccggc
     1381 tgacccactg ccgcaagcgc acgcccggca ccaccacgag ccagcccccg gtcaccagcc
     1441 aggtgccgcg caccagcatc cagacggtcg cggtcaggga ggcgctcccc tacaccgggt
     1501 cgaactccgg gccgctgctg ctcgccgccg tcagcctcct cggcgccggc accgccgtcc
     1561 tggtggccag ccgcagccgc cgccagcggg gccggatcgc ccggtagcca acgcggcgcc
     1621 ccgcccctcg cgccggaccg acaccctcgg accggccagc gccccggtga accaccgggg
     1681 cgctttcacg tttccaggcc cgggaggggt gagccgccgt tgaggaccgc ggcggcgtcg
     1741 accaggcgat aggcatagcc ctgctcggcg agaaaccgct ggcggcgctg cgcgtactcg
     1801 gtgtcgttgg tgtcgcggct gaccacggtg tagaagcggg cgccgccacc gccggccttc
     1861 ggccgcagaa tgcggccgag gcgctgggcc tcctcctggc ggctgccgaa ggttccggaa
     1921 acctggatcg ccacgttggc ctcgggcaga tcgagggaga agttggccac cttggaaacc
     1981 accacccggg ccagctcgcc ggagcggaac tgggcgaaca gccgctcccg ctcggtgttg
     2041 ggggtctgcc cggtgatcac cggggcgtcc agctctttgc cgagggtctc cagctggtcg
     2101 aggtactggc cgatcaccag cacccggtcg tcgcggtgca actcggcgag gcggcgcacc
     2161 accggcagct tggagcgggc cgtggccgcc cgccggtagc gctcctccgg ctcggcggtg
     2221 gcgtagccca tgcgctcgtc gtcggtcagg gtgacgcgca cctcgacgca gtcggcggcg
     2281 gcgacccagc cctgcacctc caggtcgcgc caaggcacgt cgtagcgctt cgggccgatc
     2341 aggctgaaca cgtcaccctc gcgcccgtcc tcgcggatca gggtggcggt caggccgagg
     2401 cggcgccgcg cctggatctc ggcggtcatg cggaacaccg gcgccggcag caggtgcacc
     2461 tcgtcgtaga cgaccagccc ccagtcctgc aggccgagca ggtcgaggtg ctggtagtca
     2521 ccgttgctcc gcctccgccg gtctcccgtg gcccggtctc ccgtggacgg cgccgcttcg
     2581 ctcgcctgcg tgctcagccg gcgtcgggtc gtgagcacct gataggtcgc gatcgtcacc
     2641 ggggcgacgt ccttgcgggc accggagtac tcccccacct ggctgggctc cagagtggtg
     2701 cgggcgagca gctcgtcgcg ccactgccgg gcggccaggg tggaggtgac caggatcagg
     2761 gtgcgggcct ggacggcggc catggcgccg aggccgacca gcgtctttcc ggcgccgcac
     2821 ggcagcacga tcacgcccga gccggcggcg gcgaaggcgg tgaccgcctc gcgctggtag
     2881 cggcgcaggg cgaagcgctc gccgctgcgg gcgtcggcct cccgccatgc ctgcggcagc
     2941 ggcgtgccct cgacatagcc ggcctggtcg tcggctggcc aaccgagctg cagcagggcc
     3001 tgcttgagcc gcccccgctc gccgagcggc accagcaccc gcgtcgggcc gaggcgttcg
     3061 ccgaccagcg gcgcgacctg cttggcgcgg accacctcgg cgagcaccgc ctcctccgcc
     3121 gcctccagca cgagctgccc cgagttgcgc ggatcgccgc ggagcgtcag ccgcccgtag
     3181 cggtccatgg tttcgaggag gtcggcgagc agcgccggcg ggacggggta gcgcgagtgc
     3241 tcggtgaggg cggcgcggac ctgggcggcc tcgatcccgg cggcccgggc gttccacagg
     3301 gcaagtgggg tgaggcggta ggtgtggacg tgctcggggc tcttctccaa ctcggcgaag
     3361 ggcgcgatcg cgacccgggc cgcgtcggcc ttggggtggt caacctccag cagcacggtc
     3421 cggtcgctct gcacgatcag ggcgccgtca gccaccatgc gccgcctctt cgccgctgga
     3481 gcgcaggcgc gcgaccagct cggccgggtc ggcggcgccc gccgcggccc tgggggcggg
     3541 atgcgggcgc agctgcgcgg ggagcgggcg ggcgtggcgg acggccttgg gggactccag
     3601 gcgtgcggtc ccgtcgggct cctccgcgag cggggcctcg ccgagcttgc gcagcaccgc
     3661 gatcagctgc cgctgggtgg cgacggtgac cgccacgccg ggggcgagct cgcgcaagcc
     3721 cagcttgcgg ccggcggcgg agcggaccac ctgggcgacc tgggcggggt cgccgcgcag
     3781 gtaggtggcg gcgtcgccga cgcgcagccg tccgtggcgc cgggcggcgt cgtcgaccag
     3841 gtactcgagg gcctggggca gcggcgtcga ggagtgctcg cgcaggaagc cgagcacgtc
     3901 gtcggcgccg agcccatggt cgaaggctcg gcggaggctc ggctcatgga gtcgccagac
     3961 gctgccgctc cccggctcgc ggtcggcgag gtaccgcagg cgcgactcca cgtgtggggc
     4021 gaggccgtcg ggcgcgacca cggtcaggtc gccggcgacc aggaagcggg tcgatccttg
     4081 ggggagagcg ccgtcgacct gcagctgctc ggccagctcc tcggcgcccg cccccgcggc
     4141 ccaggccgcg gctgccggcc cggcggcgat ccgcccggcg ggcccgtcgg cacggaccgc
     4201 caggccaagc agttcggcga ggtcggcgac ggccagcacc gtcgcgatca ggcgttgcgg
     4261 cggggttcgg accgccaccg cgcgtgcggc gaacgcggcc ccgacaaggg tgtgctcagg
     4321 agcgaagccc ggccaggtca gcggctcctg ccaggcgagc agcgcggcga gatcatcggt
     4381 atcgacggcg gcgatgcccg gtcctgccgt cgccacccct ggtcccgttg ggggcccccc
     4441 cgagttcagc ctatcaagtt cggagggggg cgcgttttgg ttgtccacag gcctggggac
     4501 cctcgcctcg gccggggcgg gcagccgggt cagggccgcg agcaggctgc ggcggcggtc
     4561 gcgggtggcg gcgaggcggt ggccgccgag ggggcgggcg cgctcggcgc cggggacggg
     4621 tggttcgtgg cgcgggagga ggtggtcctc gaaggcgagg tcgcggttgc gccaggcgac
     4681 cgccaggtcg gcccagcggc aggcgcgcga accagcgagc caggcgtcgg cttctgggcg
     4741 cagggcgagg gtgcgctggt cccaggggcc gccgacggcg agcagctcgg cctcggcgag
     4801 cagatcgatc aggaaggcca gctcgtcgtc ggccaggctc agctcacggg cgaggcggcg
     4861 caggtcgcgg accgcgaccg tgccggcctg ggtgagcggc agggggtcgc ggtcaagacg
     4921 ggtgagcacg ccctcggccg cgaccaccag gcgcagcgcg gcggcgcggg cgcggtcggg
     4981 cgcgtcgggg accgggtcga gcaggcgcgg ggtgggcgcg gcgggccact ccgggaacac
     5041 caggccgccg cgcaggccga tcgccgcctc gcgcggcacc acgaagcggc tgtaggtggt
     5101 gggcagcagc aggccgtggt cgagcagcca ggtggcgggc tcggcgtcgg ggttgcgggc
     5161 gaagcggccg tcgccgacgc cgaggatggt gagcgggccg ggtgcggtca gcgcctggtc
     5221 gagcagcgcc cgggcgggct cgccggcctc ggcgagcagg cgggccacca gcgcccggtc
     5281 ggccagcgcg gcgccgacgg cggcgaccag gtcgaccttg cgggcggggc ggtcgatcag
     5341 caggccgagg tgggccgcga tgcggtcgag ctgctcacgg gccaccccca ccttggccag
     5401 ctcggcgacg gacggcccga ggcggcccgg gttgccgaag ggggcgtgga gcccgcgcgg
     5461 cccggcgatg ctcccatcct ccgccgggag ggccaggccg agtttggaga gggcggcgag
     5521 gccctggtgc agccgttcgg ggctgaggcc agggccagcg gcggcggcca gcgcctcgac
     5581 ggtggtgggc aggccgacga gtgcgagcag ctcggcgagc tgcagcgcgg caaggtccag
     5641 ggaacgcagc gccgcgccca gcgaggcagg gctgccggcg cggctcgcga gctcggcgaa
     5701 gctcgcggga ggcggctccg ccttcgcctc cgccggcccc ccatggacgg cttcgcttcg
     5761 cccgcccgcg tgctcggcgg ctccgccggc cccccatgga cggcttccct cgcgcccgcc
     5821 tgcatgggag gccggcgtca gcagatcggg ccggcttcgc agcaggcagc cgagctgggc
     5881 gtccgacagg gacgccaacg cagccgcata tccggacacg ccgatctcct tcctagccgc
     5941 tcgctgagcc ttgccgccag caacccgcca accgcaagag gggaagctcg aaataacgag
     6001 actaccgcag acccccgact gagatctccc ccgacggccc caacgggcgg acgaggagcc
     6061 ttgcttcccg gcaacacccc cgaccccgag cccgccacgc gaagaggcgg agcttccggg
     6121 gggaagcgca gctccctcga ttcaactcac agggtacggg ttgcgaggcg ggtgacgaag
     6181 tcccgttcgt ccgggccgta ccagggtggg tcgccggcgg cggcgttctc ggtcatgcgg
     6241 cctttgcggg aggtcgcggg gatggcgacc gtgcagcggg ggtcggagag gccccatttg
     6301 agcagcgcct ggggccaggt gcggacgccg aacggctcca ggggcgcgag ctctgacggc
     6361 ttcggccagc gggacaggac ggcgccgcgg gcgaacgggc gcatcagcac cacgccgagg
     6421 tcgaggtcgg cggccagcgg caggatggtg cgctcgacct cgcgttccag ggggttgtag
     6481 gggatctgga cggcgctgat ccggccgctt cgcatcaccg tgatcaactc gtcgaaggcg
     6541 tggctgtcgt agtgggtggc gccgatggcg gtgaccaggc cgtcgtcgcg cacacgctcc
     6601 agcaggtcca ggcgggcggg ccaggcgacc aggttgtgga tctgcagcag ctccaggtgg
     6661 ccgtgggcgt actggagcga ccgctccacc tggcgttccg cctcctcgtc gtcgggggtc
     6721 cagaccttgg tggcgaccag ggcctcggcg cggcggccct ccagcccgcg gccgaggacg
     6781 cgctccgcct ggccgtacat gggcgaggag tcgaagaagt tggcagcggc cgccagcgcc
     6841 tcggccacga tccgccggca ggtggattcg gcggcgaccc cgcggacgtc gaaagtccgc
     6901 caggtcccca tgccgatgac cggtaccttg agcccgctcg acccgagtgc gcgtcgttcc
     6961 actcgcactc ctcgctgccc accgccagag gcgccagagg atgaatggtc caaaggtact
     7021 acaaatgaga gcgacctgtc tctctcatag gctatgccga ccggttacga tcggctagcc
     7081 catccgggcc cggcgccgga acgccagcgc ggtgaggctg aaggtgagcg cgcagaacgc
     7141 cagcgccgcg atgaacccct tcgagaccgt ggcgacgttg aaggcgaccc cggtctcctg
     7201 gatgaaggcg cggccggcct ccagcacgta ggtgatcggg ttgaccgaga cagcggcctt
     7261 gaaccagccc tgcatcagtg aacgcggcag ggtgccgggg gtgaggaaca gcagcggaaa
     7321 gaagatcacg aaggtggtct gggtcgcccg caccgagccg gtggcgagcg cgaccgccac
     7381 gctgaccgag gcgaagccga ccccgaacag gaccgtcagc gccagcaggc cgagcgcgcc
     7441 gcccagctcg ccgcccgcgt agcggacgcc catcgccagg ccgagcaggg tcagcagcac
     7501 gctgtagctg accaccgaga acatctcgcc gagcagggtg ccgatcacca tcgcggggcg
     7561 gcgcaccggg gtgaggctga gcttgtccca gtagccgccg tcgatgtcga ggaccagcga
     7621 gttcccgccg gcggtgttgg ccgaggcgaa cagcaccgcc accgggagct ggaaggcggc
     7681 gtagttcttg atgccgaagg ccgggccggc gatcttgccg agggcgccga tctggaccac
     7741 gaagaagaac agcgggatcg ccagcgccgg gatgaacgac tccggctcgc ggagcagcgc
     7801 ccggatcgaa cgggtcgtca gcgccagcac gtccgaggcg aaccccgacg gtgtggccag
     7861 ggtgcgttcg ctggcgggag cgggcgcggt ggcggcggtc atgccgatcc ccttccagtt
     7921 ccggtccctg cgccggtccc tgcgccggtc cctgcgccgg tggtggcgcc ggggcggtcg
     7981 ccgtcgctgt ccctgtccct gcgctggtcg ccgccgtccg ggctgctctc ctcggcggcg
     8041 cgactggcca tgcggccgcc ggtggcggcg aggaagacgt cgtcgagggt cggccgcgag
     8101 acgctgatcg ggccggtggt gatccccgcc cggtccaggc cgcgcagcag caccgcgacg
     8161 ttgctgggcc cgtccgggac gaacagggtg tagccgctgg cggtgccgcg cagctcgccg
     8221 gctgggagtt cgccgcgctc ctgcatctcg aggatggtaa cgcgggcggc gccgcactcg
     8281 cggtcgtcca cggcgacgtc gatcacgtcg gcgccgacct ctgccttgag cgcggccggg
     8341 gtgccctggc ggacgatctg gccgcggtcg atgatcgcca cctggtcggc gagctggtcc
     8401 gcttcctcca ggtactgcgt ggtaaggaac accgtggtct gatagcgctg gcgcagctcc
     8461 cgcacccggt cccagatgag cgtgcgggag gtcgggtcca gcccggtggt cggctcgtcc
     8521 aggaacagca cgcgagggcg gtgcagcagc gccgaggcca ggtccaggcg gcgcttcatg
     8581 ccgccggagt agcccttgat gcgccggtcg atcgcgtcgc tgaggccgac cagctcggtc
     8641 acctcatgga tctgttcggc gaggaccgcg ccgcgcaggc cgtacaggcg ggcctgcagg
     8701 gtgagcagct cgcggccggt ctgcttgggg tcgaggcctg cctcctgcag tgccacgccg
     8761 atcaggccac gcacggtggc ggcctcctgg acgacgtcgt ggccgaggac cgagccggtg
     8821 ccgctggtcg gttcgagcag ggtggtcagc atgcggacgg tggtggactt gccggcgccg
     8881 ttggggccga ggaagccgaa gatctcgccc tcttcgactt cgaggctgac gccgcggacg
     8941 gcttcgacct cttcgaaacg ccggacgagc ttggttgcct gaatcgccgt tgccatgccc
     9001 cgagtgtaag ggagacccgc gacagcgcga cttgattttg agaccgcgtg gtcgcggacg
     9061 ggccttctgg gtttgcctgc ccccctcagc gtgaacgcct tgctggtggg gaaccagcga
     9121 agtggagggg accttgcgga tccaactgga cgccctggct gccagcatcg gcggcctcgg
     9181 cgagctggac gtggagcggg ggttcgcacc tgcactgcag cgagtggtcg acgcggccaa
     9241 gaagttgttc ggagcgggag gcgccgggct gatgctggtg gacgccgacg gcgtcctgcg
     9301 ctgggcgagc gcctccgacc aggccgcgca gacggtggag gacagccagg aacggctcgc
     9361 ccaggggccg tgcctggagg cgttcgagca gcaccgggtg gcctacctgc gcgacgtcca
     9421 cgccgagccg cgctggcgtg agctggccca ggtgatggcg gcggagagga tggtcgcggc
     9481 gctgagcgtg ccggtggagc tggatggcgg cctgatcggc accctcgacg tctacttcga
     9541 catcccctgg gagtgggacc cgagcgaggt cagcgccctg cgcgcctacg cggcggtggt
     9601 ggcgagcctg ctgcaacatg ccatggtcgc gcacgtcaag ggccggctcg ccgaccagct
     9661 ccaggtggcg ctcgaccacc gcagcctgat cgagcaggcc aagggcgtcc tgatggagcg
     9721 ggagggtctg gaccagaccg ctgccttcga acgcctgcgc cgcaccgccc gctcgcagcg
     9781 ccgccgcatc gtcgagctgg cccgggaagt cgtggacaag acccagctcc cgtcgtaacg
     9841 cctcgtaccg ctggagtgtc tgccggagcc tcttgagggc ggggcctgct accgccaggc
     9901 gggggcctcg ccggacccgg cgaaggcggg ggcgacctcg cgggcgatcc gctccaggtt
     9961 ggcgcaggcc gcctcgtggc tgatgccggc ggggcggctc cagagggcga cgtgggtgaa
    10021 ggggagctgc gcctggaact cgcgcagggc ggcgacgacg ccgtcggggg tgtcgcagaa
    10081 gaccgcggag cgcttcaggc gggccgcgtc gatcggggcg gggcgcggtt tgccgcggtc
    10141 ggtgctccag tcgccgtagc ggtcgagctg gtcggcgatg gccggggcca gttcggtggc
    10201 ccaggtgcgc tccgggtcgt cgctgacccg cagatggacg ccgaccacga tcgggaagcc
    10261 ctcggccggg cggccggcgc gctccagctc cgcgcgcagg accgggtacc gctcggcggt
    10321 cttctgccag cggaagtggg caagggcgaa ccagccgtcg ccgatccggg cgacccggtc
    10381 gagcgcccgc tcggcgccgc cgccgaggta gagcggcggt ccggacagtt ggacagggcg
    10441 tggcccgacc ggcaggtcgg tgccgtccgg cccgtcggcc gcgacgcctt cggcgaaggc
    10501 gcggcgcaac caggcgacct tctcctccag caggcggccg cgctgcccac ggtcgacggc
    10561 gaaggcgtcg aactcgcgct cgacgtagcc gccgccgaag ccgaggatca gccggccgcc
    10621 gctgaccagg tcgaccacgg ccgcctgctc ggccacgtcc cggggccggt acaggggcag
    10681 cagcagcacc ccggtgccga cgcggacgcg ctcggtgcgg gcggcgaccg cggccaggaa
    10741 cgggagctgc tgcgacaggt gggcgtcctc gacggcgtgg tgctcgctgc cccacacggc
    10801 ggtgaagccg agctgctcgg cgagcacggc ctcgtcgagg cgctcgcggt aataggcggg
    10861 gtcgggctgg cggccgcctc ccggccgcag ctccgtccag atcccgaagg tcgccggtcc
    10921 tgtgggcatg ggtacgaccc taccctaccg ggccagggtc atggcgaggt catggcgagg
    10981 cgagcaactt ggggccgcgg cgcagcggga tgccgtcgtt cggttccagg gtgatgccca
    11041 tcttggggaa gcgggccagg tcggcggcgg tgaccatgtg gacggacgca cttccgtccg
    11101 gtcccaccgt gaaggtccca gcggagatcc ggttctggtt ctggagggtg tcgcccggcc
    11161 cgacgaacca gcactcgtag acgtggccgg cgtcggcggg cggcaggtgt ttgacggtga
    11221 ggtcaaccac cagcccggca cgggtgcggt ggacgcgggc gtgaccgctg gcgggcccgc
    11281 cgttcggggc ggccagcgcg aagttggtgc cagggcccgg cccgaacagg cgaatcccgg
    11341 tggccaggcc gccgaccagg atcaccaccg cggcggccgc gacggccagc cagcgcagcg
    11401 agcgccgcgc tcccggacgg gccgtccgcg ctccagcctg agcggctgcc tcggattccg
    11461 cttcggcggc ggcgcgatcg accgccgcga aggtccgttc gcgcagcccg ggtggcagct
    11521 cgacgggcgg ggtcgcccgt tcgagcatgg cgggaaggcc ctccagctcg gccagctcct
    11581 gtcggcactc cgggcaggcg gccagatgcg cctcgaacgc gccgacctcg ccggggtcga
    11641 gcgcgccgag cacgtagccc gcgaggtccg ggtgcggtat gacgtggtca ggcattgatc
    11701 cctcgctcct ggagcgcgag cttgagggca cgcaacgcat agtaggcccg tgttttcacg
    11761 gtacccaagg ggaggccgag gagggtggcg atctctgcct gcttgcggtc ctgaccgatg
    11821 aacaactcca gcacctgctg gtgggcgggc gagagcgact gcagggcgtc gcgcacggcc
    11881 agcccctcga tcaggcgctc gacctggtcg tggccctgct cgccggcgat cgcctcgggc
    11941 gggggcggct caaacggccg ggaggagggc cgccgccaga gatcgaccgc gatccggcgg
    12001 gcgatggtga acaggaaggc gctgacgctg ccgcgtgccg ggtcgaagcg gccggcctgc
    12061 tgccacaggc gcacgaaggc ctcctgcacc agctcctcgg cgagcccctg gtcgccgagc
    12121 agccgcaggc caaggccata gagccgcggg ccgtagcggc ggtagagctc ctccagcgga
    12181 gcatcgcgat cgccggcggc gacgctggcg agcaggtctg cctcgtcagc ggcggtctgc
    12241 ccggcggcgt cgagcatgcg ttctgacacg atccgaaagt gaaccgtgcc ggagatcacc
    12301 ctgacaaggc atatctgccg gattgacatc tggtacgggg cttggacgag cacgcctggt
    12361 gatacggaca gacgcgccgg gaagttcggc ctcccggacg gggatcccga aagcgatccc
    12421 gaaagcggat cccggaagga tccagggaac caccgggggc gcggcgcggt tgaacccatc
    12481 acgggggact tcatcggggg aaggcatcgc acccaacgaa gtccaggagg ttcgattcga
    12541 ttggtaggca ggatgtggta ggcaggatgc tcgtcgcagt cagcttgctc tccctcaccg
    12601 ggatgctcgc ggccggatgc gccagcaagt ccgggtccgg ttcggggtcg gggtccgggg
    12661 gcctgtacgg caactccaac gcgcccacga ccacagccgc tgccagcggg agcgggagca
    12721 ccgccagctc cagcggctcc agctccggcg ggcgctacgg ctccgggagc ggcagcggga
    12781 acacgggcac ggccagcgcc accatgaccg ggaccaaggt gacgatcaac aacttcgcct
    12841 tcggcccggt cgccctgaag gccaagatcg gccagaaggt gacctggacc gacgcgcaga
    12901 tgggggtcag gcacaccgtg accgccaaca gcggggcctt caaccacctg ctgacgccgg
    12961 ggacgagctt ttcgttcacc ttcatgaagg cgggcaccta cgcgtaccac tgcaacatcc
    13021 accccacgat gcacgggacc gtggtggtga cctgagaagc ctggcgccgc gccttggtgc
    13081 agcgccagcc atgcgctctc gttccggggt gagagcgcat acggacgggg gccgcgccta
    13141 atcgccgcgg cgcggccccc gtcatctctt ttcgaggctg gtcagttcgc gggtgcgaac
    13201 ttgtccggga actgcttggc gatggcggcg gcgagcgtgt caccgatggt gaacgactgc
    13261 tcgtaggcgc cggccatggc gctggtggcc acgatcgtgc tcttggcgta ggtggcgtcg
    13321 accgcgcccg ccgcctggct gacctgcttg gtcagcagcc ctgccagctc cgatttggag
    13381 aggctcgggt tggccccggc gaggaagccg gcgaggctgt cggagaagct ggcgagcgcc
    13441 gcctgcgact tcttgacctt gccctggtcg ccggtggcct tggcggcggt gtagttcagc
    13501 agggcgctga ggtggtcgtt ccacagggtc ttgaacttgg tcccggcggc gcggccgtag
    13561 accgaggcga ccgactgggt gagcgcctgg gtgttcttgc ccagctcggc gccgaacgcc
    13621 ttgccgtcgg gcgcgccgct gatcgccttg ccgagcacca ggctggtgag cgccgcctgc
    13681 tcgccgcgct gcatgtccag ggtggtgcgc aggtcgtcgg gcttggcgct gagggcgccc
    13741 gtccgcgggg cggcgtcgcc accgaccgcg gccagggccg acccggtggt gaacgcgtgc
    13801 tcgtagacgg cggcgaactg gctcgccgcc tgggcctggt cgtgacgcac gctcgcgtcc
    13861 accgccgcga ccatcccggc gaactgctgc ttcaggtcgc tcgccacggc ctgcttcggc
    13921 agcttcgggg cggtggcggc catgaacgtg acgaactggg cctggtaggc acccaggtcc
    13981 ttcagtgcct tggcggcgcc gggctggtcg cctccgccct tggcgaccgt gtagcggacc
    14041 aggatgccga cctggttctt cagttgctcc ttgaactggt tgccggcgct ggtgccgaac
    14101 gccgacccga acgaggcccc gaggtcgtcg gtgttcctgc cgaccagcag cgccgcctca
    14161 ggagcagccg ccggctgccg gttgatcatg tcctgcagca tggccacgct gagaatggcg
    14221 tgctcgccga gctgcttgtc gaactggatc cgcaggtccg ctcccttggc cgcgcccgag
    14281 ccgcccccgg atgccgggcc gccactcccc ttgcctgacg agcaggccgc cagggccagg
    14341 gcgaccagtg ccccggccgc cagcaccttc accgtgcgca tgcgcgcctc cccatattca
    14401 tcccttcctg ccgagtccta cgaaataacg gacagccccg gttcgcgcca ataaacgagc
    14461 ccgccccatt ccagcgagaa attcacgcgc tcgaaatact ggaagcccct gggtggataa
    14521 ccattacccg aagacctgcc cggggtccag tccggtttct attctctcga agctggcgtc
    14581 ccgtttccgc cggcattccg ggaattgctc aggtgtagcg cgggaagtcg tacggatagc
    14641 cgttctcggc ctcggcgcgg gcgagctggg cggcgaggcg ggcgaggaag ctgtcgacct
    14701 cgtacatggc gtagccgcgc gggcccagcg tgaagcgggt gacgccgatg tcgtgggcgg
    14761 tgatgcgcgg ggcgctgtcg gggttgtcct cgacccgggc cagctcacgg gcgaccaggg
    14821 tgatgaagcg gtcgacctcg tccatccggt agccgcgcac gattcgggtg aaggtggtgg
    14881 aggccacgtc gctggcggtc atccggtcgg gcagcggctc gacccgcggg gtcagctcca
    14941 gctgcggctg cgccacccgc accatctcgg cgacgagccg gtgcatgcgc ccgaggtcgg
    15001 cggcgaccag gtcgaggaag tcgtccacct cggccatggc gtagccgcgg aaggcgcggt
    15061 ggaacagctt ggtctcgacc tcgtccgcgc cgagcagccc cgccggcgtg cgggagccgg
    15121 acgcgtagca ctccgccagc cgggccaggt cgtcggccgc ctgggcgagg aactggtcga
    15181 cctcgcctgg ccggtatccc tgccgcgccc tggtgaacgt cttgtccctg atcgcttcgg
    15241 gagtgagccg gaccccagcg gtcatcgcgc ctgcatcgcc tctggcctcg tcagttgtcg
    15301 tccgtcacga gactacacaa aagcggggtc caaggggatg gctgggcagt gtctcgggca
    15361 cccactacag tctgagggag cgaccagagc gagcgcggac aaggagaggc gaacggatga
    15421 gcacgcgacc ggcccgggcg gaagcggcgc cgcccgccga gcagctcgac ccgcgccgct
    15481 ggctcgccct cggggtggtg ctgatcgccg cgttcatgct gctgatcgac atcagcatcg
    15541 tcaatgtcgc gatcccatcg atccagcgcg acctgcacgc cagctactcc caggtccagt
    15601 gggtcctggc cggctaccag ctggccttcg cggtggtgct gatcaccggc gggcggctcg
    15661 gggacatcta cggccgcaag cgcctgttcc tgatcggggt gaccggcttc acggcggcct
    15721 cagcgctgtg cggcgcggcg cagaacccgg ccatgctggt cggctcgcgc ttcctgcagg
    15781 ggctcatggg ggccctgctg ttcccgcagg tcctgtcgat catccaggtg gaattcgcgc
    15841 cgcgcgagcg gggcaccgcc ctagggatgt tcggcgcggc gatcgggctg gcgaccatct
    15901 ccggcccgct ggccggcggc ctgctcatcc agggcaacct gctggggctc gactggcggc
    15961 cgatcttcct cgtcaacctg ccgatcgggg tgatcgccgt ggtcgcggcg gtggtgctgc
    16021 tgcgcgagtc gcgcgccgag gacgccccgc gcctggaccc ggtcggcgtg gtgctggcca
    16081 ccgccgcgct gctcgcgctg gtctacccgc tggtcgaggg ccgcgacctc ggctggccgg
    16141 tgtggatctt cgtgctgctg gccgcgtcgg tgccgctgtt cgcgatcttc gccgcctacg
    16201 agcggctcaa gacccgcagg gacaactcgc cgctgctggt cctcgacctg ttccgcgaac
    16261 gggcgttcgt gagcgggctg ctgctcagcg cggtgttctt ctccggtatc gccgcgttct
    16321 tcctgacctt ctcgctgttc ctgcagatcg ggctcggctt caccgctctg cacgccggcc
    16381 tcaccacgat cccgttctcg ttcggcacgg cggtcgcctc ggcggcctcc acccggctgg
    16441 cgccacggct gggccgccgc gtcctcagcc tcggctgcct gctgctggcc gcgggcatgg
    16501 gcggggtgat cctgaccgtc tcccactacg ggacggcgct gcactcctgg cagctgctgc
    16561 cggcgctgct gctctgcggg atcggcctcg gcctggtggt ggcgccgctg gtgaacgtga
    16621 tcctggccgg catcagcggc cgcaacgccg gctcggcctc cggtgcgctc accaccatcc
    16681 agcaggtcgg cggcgccatc ggcgtggcga tcatcggggt gatcttcttc gggctgctgt
    16741 ccagcgaggc gaatcccacc gtcgaccagg tcgccccgca gctccgctcg gggcttgaga
    16801 ccgccgggct gcccgcgccc gccagcgacc aggtggtcgc cgggttccgg gtctgcttcc
    16861 gcgaccgcgc cagcgccaag gatcccacct cgaccccggc cagctgccag cgcctccagg
    16921 gccagagcca aaaccagggc cagagcggct ccggcggcgg gcaggcgcag ggccagcagg
    16981 ccggccaggc gatccaggcc ggcgcgaccg ccgcgcgcaa gcaggatttc gtcagctcgg
    17041 tcgagcgcac cctctggtac gaggtggccg tctacgggtt gagcttcctg ctggtgttcc
    17101 tgctgccctt cgtcccgggc caccggctgc ggccgggcgg cggaccagcg gcggcctgac
    17161 ccggcccgcc gcccctcctg ccctactggt gcaggggggt ctcgtggccg cggtgctcgg
    17221 gctgctccag ctggaaggtg gagtgctcga tgtcgaaatg gccgccgagg cactcgcaga
    17281 ggtggtcgag cacggggccg gcggtggcgc cgccggccag cacgacgtgg gcggagacca
    17341 cgttcatgcc gctggtgatc gtccagacat gcaggtcgtg gacgtcggcg acgcccgggg
    17401 cggagaggat gtgctgacgg acgtcggcca ggtcgacacc cttgggggtc gcctccagca
    17461 gcacgtcgac cgcatcgcgc agcagccgcc aggtgcgcgg caggatcagc aggccgacca
    17521 gggccgaggc ggccgcgtcg gcgcgctccc atccggtggc cgcgatcacc acggcggcga
    17581 ccaccaccgc cacggcgccg agcaggtcgc cgagcacctc caggaacgcg ccccgcagcg
    17641 gcagcgcctc gccctgggca cggcgcagca gcagcagcga gccggcgttg ccggcgagcg
    17701 ccaccatccc gaaggcggcc atcacgccgc tggcgacctc gggcggtgcc agcaggcggc
    17761 gcaccgcctc gaccaggacc agcgcggcga ccccgaacag cagcacgccg ttgaccaccg
    17821 ccgccaggat ctccagccgg aagtggccgt aggtccgggc cggcgtggct ggtcgcgccg
    17881 cgaaggcgat tgccagcagg ctcatgccga tcccggccgc gtcggcggcc acgtggccgg
    17941 cgtcggccag cagcgccagg ctgccggcca gcacaccgcc gaccacctcg gccacgagga
    18001 tcgccagggt gatcgccagc accgccagca gccggccacg gtgcgccccg gccgcgctcg
    18061 ccggcgactt gagatgatcc tggtggtcgt gtgtcaccgg gcgagtgtgc cacggcgacc
    18121 ctccacctga ttggctggag ggcggacctg gtcaggcctt cctggtgaag gcgctcacgc
    18181 cggtgatctc gcggccgacg atcaggctct ggatcgagtc ggtgccctcg taggtgaaca
    18241 ccgcctccat gtcggcctgg tggcgggcga cgtggttctc cagcaggatg ccgatgccgc
    18301 cgaggatgtc gcgggcgtcg gcgaccacgc ggcgggcctt ggcggcgttg tgcatcttgg
    18361 ccagcgacga catgccgtcg gtcatctgcc cggacccggc gagctgcgac aggcgcaggc
    18421 agatgagctg catcgcggtg atctcggcca gcatggtggc gagcttgtgc tggaccagct
    18481 ggtagctggc gatcggcgcg ccgaactgct cgcgctgctt ggcatgggag agggcggact
    18541 cgtaggcggc catggcgtgg ccgaccgcct cccaggcgac cccgaaccgg gtcgcggcca
    18601 ggaccttggc ggtgtcgccg aagccgttgg cgccggccag gcggttggca acggggacgc
    18661 ggacgccctc cagggtgatc tcggcctgcc agaccgctcg cttggcgatc ttgccggtca
    18721 tcacgctggc gttgaatcct ggcgtcccct tctcgaccac gaagccgccg accttgccgg
    18781 cctcgtcgcg ggcccagacg atcaccaggt cggcgaagct ggcgttgccg atccagcgct
    18841 tggcgccgtc caggacgtag cagtccccgt cgcggcgggc gcgggtctcc agggcgaccg
    18901 cgtcggagcc gtgctcgggc tcggtgaggg cgaacgcgcc gatcttgtcc atccgtgcca
    18961 tggcggggag ccagcgccgc ttctgctcct cggagccgag cagcgcgatc gactgcatcg
    19021 ccaggccgga gtggaccccg aagaaggtgc agacgctgcc gtccgcgcgg gccagctcca
    19081 tggcgatcag cccggtggcg accggcgaca ggccgggaca gccgtcgccc tcgatggtgc
    19141 cgccggcgac gccgagctcg gcgagcttcg ggaccagctc gaaggggaac tcggcccgct
    19201 cccagtagtc gttgatgatc ggggtgacct ccttgtcaca gaaggtccgg acccgctcgc
    19261 ggacgtcgcg ctcccgctcg gtcagcaggt cgttcatacg gtagaagtcg gtcacgtcat
    19321 gttcgctcac gcgttgttcg cttccgcgct cagtcatttg acgatccctt tcggtcagcc
    19381 agtatcggct ttcccggcga aggtagcagc taaagaggtt cgcggaatag gttgaggatc
    19441 atttctcaca gctccagccc gcagtaccgt agaaacgaga acaacagcgt ggactcccga
    19501 cgaacccggg cgatgccctg ccaggcggcg tccaggactt ctgcgatc
//
LOCUS       DASWDC010000350         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_238697, whole genome shotgun sequence.
ACCESSION   DASWDC010000350 DASWDC010000000
VERSION     DASWDC010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238697"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VF995_11225"
     CDS             complement(<1..636)
                     /locus_tag="VF995_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX9344171.1"
                     /translation="MSLVLVCGGTRSGKSAVAEQLAAAAGAPVHYVATGATTDLEMGE
                     RIARHRDRRPAGWRTVETLDPLDALGAADEATVLVDGIAPWIARLMTAEGLWTAAEVA
                     PLGPNGRAAHDRALAQVGQFAAAAATRPALTIVVAEESGLGVVPQGAATRRYLDLAGD
                     AAQALAAVAERVLLVVAGRILDLDPADRDPSQRRPAAVGTAVGTAVGTAAAN"
     gene            complement(636..1577)
                     /locus_tag="VF995_11230"
     CDS             complement(636..1577)
                     /locus_tag="VF995_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HEX9344172.1"
                     /translation="MNHTVKRPPALLLAAHGSQDEEAARELEQLTKLVATLAPAGVAV
                     ASGCIELAAPSVPDALAELIADGAAEVAVVPLMLFAAGHAKNDIPALLTRCRLEHPGV
                     RLRYGRHLGLHPDLLALVDQRLDEVIPEAERPETAVLLCGRGASDPDANADVAKLARL
                     LWEGRPWPLVESCYTGITTPTVPDGLERCRRLGATRLVALPYLLFTGRLEERVQQQCA
                     VFAAAHPEVAVRVAARLGPDPRIARLVVARYQEILEGDPRMNCDLCVHRVALPGFAHK
                     VGAPATPHAHPDDPPHHHHHHHDQHPQAHAELDRHGR"
     gene            complement(1580..2260)
                     /locus_tag="VF995_11235"
     CDS             complement(1580..2260)
                     /locus_tag="VF995_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEX9344173.1"
                     /translation="MPHRSPHPIERESYRILRALVDLPALGLGPLSCAVAERVIHASA
                     DLGYATDLVLNEGALTRGLAALRGGVPVVVDARMVAAGITRLPTRCALDLDQTASDPT
                     ASDPAARDPAASATTRAAAGIRAAARQVGPGAIWVIGCAPTALVELLDLRPDPSLVIG
                     LPVGFVGAVEAKHALAASGLPAVTNRSPKGGSAVAAAALNALLYLDLDLDLDFDNDLE
                     PLEPEGEP"
     gene            complement(2277..>2478)
                     /locus_tag="VF995_11240"
     CDS             complement(2277..>2478)
                     /locus_tag="VF995_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019982890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HEX9344174.1"
                     /translation="ELLLAHRHARTPVGIVTDAFRPGERVALTTLGDLDPAMVGMTTV
                     VLVGSSRTQVIAGRMVTPRGYR"
BASE COUNT          302 a          836 c         1033 g          307 t
ORIGIN      
        1 atttgccgcg gccgtaccca cggccgtacc cacggccgta cccacggctg cgggtctgcg
       61 ctgggatggg tcgcggtcgg ctgggtcgag gtccaggatc cggccggcga cgaccagcag
      121 cacccgctcg gccacggcgg cgagggcctg ggccgcgtcc ccggcgaggt cgaggtagcg
      181 gcgggtggcg gcaccctgcg ggacgacccc gaggccgctc tcctccgcca ccacgatggt
      241 gagcgctggg cgagtggccg cggcggcggc gaactggccg acctgggcga gggcgcggtc
      301 gtgggcggca cggccgtttg ggccgagcgg ggcgacctcc gcggcggtcc acaggccctc
      361 ggcggtcatg agccgggcga tccacggcgc gatcccgtcg accagcacgg tcgcctcgtc
      421 ggcggcgccc agcgcgtcga gcgggtcgag ggtctcgacg gtgcgccaac cggcggggcg
      481 ccggtcgcgg tggcgggcga tccgctcgcc catctccagg tctgtggtcg cgccggtggc
      541 gacgtagtgg accggagcgc cggccgcggc ggcgagctgc tcggcgaccg cgctcttgcc
      601 cgaacgggtc ccgccgcaga ccagcaccag gctcatcagc gcccgtgcct gtccagctcg
      661 gcgtgggctt gcgggtgctg gtcgtgatgg tgatggtgat gatgtggcgg gtcgtcaggg
      721 tgggcgtggg gcgtcgccgg cgcgccgacc ttgtgggcga agccgggcag ggcgacccgg
      781 tgcacgcaca ggtcgcagtt catgcgcgga tcgccctcca ggatctcctg gtagcgggcg
      841 acgaccaggc gcgcgatccg ggggtcgggg ccgaggcggg cggcgacccg gacggcgacc
      901 tcgggatgcg cggcggcgaa gacggcgcac tgctgctgga cgcgctcctc gagacgaccg
      961 gtgaacagca ggtacgggag cgcgaccagg cgggtggcgc cgaggcgccg gcagcgctcc
     1021 aacccgtccg ggacggtcgg ggtggtgatg ccggtgtagc aggactcgac cagcggccag
     1081 gggcggcctt cccacagcag ccgggcgagc ttggcgacgt cggcgttggc gtcggggtcg
     1141 gacgccccgc ggccgcacag cagcacagcg gtctcggggc gttccgcctc ggggatgacc
     1201 tcgtcgagcc gctggtcgac gagggcgagc aggtccgggt gcaggccgag gtggcggccg
     1261 tagcgcaggc gcacgccggg atgctcgagc cggcagcggg tgagcagcgc ggggatgtcg
     1321 tttttggcgt ggccggcggc gaacagcatc aacggcacca ccgcgacctc ggcggcgccg
     1381 tccgcgatca gctccgcgag cgcgtccggc accgaagggg ctgccagctc gatgcacccg
     1441 gaggcgaccg ccacgcccgc gggcgcgagc gtggcgacca gcttggtgag ctgctccagc
     1501 tcgcgggctg cctcctcgtc ctggctgcca tgtgcggcga gcagcagcgc cgggggccgc
     1561 ttcacagtgt ggttcacggt cacggttcgc cttccggttc cagaggttcc aggtcgttgt
     1621 cgaagtcgag gtcgagatcg agatcgagat acagcagggc gttgagcgcg gcggcggcga
     1681 ccgcggaacc gcccttgggg ctgcggttgg tgacggctgg caggccgctg gcggccaggg
     1741 cgtgcttggc ctcgaccgcg ccgacgaacc cgaccggcag gccgatgacc agagacgggt
     1801 cggggcgcag gtcgagcagc tcgacgaggg cggtcggggc gcagccgatc acccagatcg
     1861 cgccggggcc gacctggcgg gcggccgcgc ggatcccggc ggccgcgcgg gtcgtggcgc
     1921 tggccgccgg gtccctggcc gccgggtcgc tggctgtcgg gtcgctggcc gtctggtcga
     1981 ggtcgagggc gcagcgggtc ggcaggcggg tgatcccggc ggcgaccatg cgggcgtcga
     2041 ccaccaccgg cacgccgccg cgcagcgcgg cgaggccgcg tgtgagggcg ccctcgttca
     2101 ggaccaggtc ggtggcgtag ccgaggtcgg cggaggcgtg gatgacccgc tcagccaccg
     2161 cgcatgacag cgggccgagg ccgagcgcgg gcaggtcgac cagggcgcgc aggatgcggt
     2221 agctctcccg ctcgatcggg tgcggggacc ggtgcggcac gctcaccggt cggccctcac
     2281 cggtagcccc tcggggtgac catgcggccc gcgatcacct gggtgcggct ggagccgacc
     2341 agcacgacgg tggtcatgcc gaccatggcg gggtcgaggt cgccgagggt ggtcagggcg
     2401 acccgctcac cgggccggaa cgcgtcggtg acgatcccga ccggggtgcg ggcgtggcgg
     2461 tgggcgagca gcagctcc
//
LOCUS       DASWDC010000351        10334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1572871, whole genome shotgun sequence.
ACCESSION   DASWDC010000351 DASWDC010000000
VERSION     DASWDC010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572871"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VF995_11245"
     CDS             complement(<1..449)
                     /locus_tag="VF995_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9344175.1"
                     /translation="MGEAIVFGPDEGEALRARGSVMLFKAIAASTGGAFSLHQRDLPP
                     GGRRPPVHRHTNCQEAFFVLEGDVEFVLGDGEASPVRRGPGTFVLVPGGVPHTFGNPG
                     PGAARVLVLHAPAMDQYFRELQALWSGDTPPDRDTELDLMRRHGMEPG"
     gene            complement(455..1609)
                     /locus_tag="VF995_11250"
     CDS             complement(455..1609)
                     /locus_tag="VF995_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9344176.1"
                     /translation="MGFELEPEQEEFRKIVRSFAEEVVAPRAAAMDREERLDEAVLEQ
                     MAGMGLFGLPFPEEYGGMGTDYVTFCIAVEELARVDSSVALTLEAAVGLGAMPIWRHG
                     TEEQKRRWLRPLASGEGIGAFGLTEPGGGSDAGALRTRAIFDADAGQWVIDGTKAFIT
                     NAGLPRARLVTVAAVTGERADGRGEISNIVVETGIPGYTVGKAYSKVGWHASDTRELV
                     FEDCRVPAGHLLGERGQGLANFLRTLDEGRIAIAALATGLSQGCADECVKYARERRAF
                     GKPIAAFQAVAFKIADMEAQAHVSRLAWRHAAAKLVAGQPFAKEAAIAKLHASEAAVT
                     CAREAVQVHGGYGYMNEFPVARFFRDSKILEIGEGTSEVMRILIARSLGL"
     gene            1971..2204
                     /locus_tag="VF995_11255"
     CDS             1971..2204
                     /locus_tag="VF995_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX9344177.1"
                     /translation="MGGAQRTNKMSITVPSEVAAELRARAGHGNVSAYVTHALLRQLE
                     HDRLGDLVAELEKLHGPLSDEELAAARAEWPKE"
     gene            2246..2635
                     /locus_tag="VF995_11260"
     CDS             2246..2635
                     /locus_tag="VF995_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9344178.1"
                     /translation="MLDNEGVSRAATDRVMYARLRAAHLDGRRVVTSAAILAEILRGG
                     ARDAGIYRVLSGVAVEPVTREIGERAGNLIGTARLGTEQAVDAMVAATALEQAEPGLL
                     VTSDVPHLSALVAEHERVAVVHADKTG"
     gene            complement(2668..4230)
                     /locus_tag="VF995_11265"
     CDS             complement(2668..4230)
                     /locus_tag="VF995_11265"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX9344179.1"
                     /translation="MLVEATGRLRRAALRDAVVQLWLPPYLLIAAVAVSDLLLRNGLS
                     TGAFLVLAPLLASRLEAGRAGILASGLVALAVALLLGEFHHPGQDTQLGIRLLLIAAA
                     IPIALVNQGAHRRERSTLGRAADTITIAASLAAGLETGQAYRLLARSARSLYAASAVA
                     VYRRQGSLLLAVAQDRADLAPPLPDQLSADEFPDAFSEEPLQAFGPPALPAVPPRQRA
                     SEIRMLQARGFAGLLWLPLVHPEGRQIGTILLAWRHRRPRLSAHDLEVSRRFAGLGAR
                     AISGSERVKAQADLLRQLQALLLTTPPAWAGGYRIGVRYQSASGLAQIGGDFYDVAEV
                     DGKGLAFILADARGKGVEASSMAAVLKGAFRTLAGEGAGPARTLARLDRLVAREGGDE
                     DFVTALAGRLYQNGRVVLASAGHPAPLGAGPPLVPRAAAPLGLGSDPLDAYGLLRPGQ
                     RLICYTDGLVEARDHAGRFIDRARLDAAVSAEQSDLQAVLDSLVALVADHADGRYADD
                     LALLGLEYCPGG"
     gene            4444..5412
                     /locus_tag="VF995_11270"
     CDS             4444..5412
                     /locus_tag="VF995_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007629504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9344180.1"
                     /translation="MRTLVTGGAGFIGSTLVDRLAGGGHEVVVVDNLSSGRVENLADA
                     LASGRVHLAEVDITGPDLEKVVAEARPEVVYHLAAQIDVRRSVADPVFDARVNVLGTV
                     ALARATLDADCRRLVFASSGGTVYGEPDPASLPVDERHPALATSPYGVSKRTAEDYLA
                     SFAPLYGLEPVSLRLGNVYGPRQDPHGEAGVVAIFCNRLLSDEPVTIFGDGLQTRDYV
                     FVEDVVDALLAAGERDEAVGARVNIGTGIETSVLDLYQALREVTGFGPEPQLAPPRPG
                     ELQRIALDCGAAERTLRWQPATTLTEGLSRTWDWAFQQVNAGSVGG"
     gene            5409..6185
                     /locus_tag="VF995_11275"
     CDS             5409..6185
                     /locus_tag="VF995_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018541781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9344181.1"
                     /translation="MNPIGVEVAEGIATVTLQRPGALNAISTELALALAEAVEPLATK
                     PSVRALVLRGAGERAFCVGADLRERGGFDDHAWFVQREVFRRGFGALRRCPLPTVAAL
                     FGFALGGGMELALACDLIVAADDAVFGLPEVRLGLVPAGGGTQLLVRRVGRSAAKDLV
                     LTGRRVDAAEALRLGIADRVVPRAELPDAARALAAEIAANAPTAVRLAKWALDLGADL
                     AQEAAIEVEDQAWRRAVLSGDRREGIAAWNERRDPRWPAS"
     gene            complement(6237..6650)
                     /locus_tag="VF995_11280"
     CDS             complement(6237..6650)
                     /locus_tag="VF995_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344182.1"
                     /translation="MEITLGPFDPALHVRSRDHYEAVRREIQLLELAADSVPRRFGEL
                     LDRIRAQVPEDTVDEAADRAFLAGEPAFQASVVIPEAQVPAALDATTQLQQLFDQFDR
                     WAAASRNQLFEAPDEVKRYRRTYLAQLRSQLAAAG"
     gene            complement(6660..8414)
                     /locus_tag="VF995_11285"
     CDS             complement(6660..8414)
                     /locus_tag="VF995_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9344183.1"
                     /translation="MKAGMRAVLVANRGEIAVRVLRACREEGLAGIAVYAEPDAGALH
                     VREADRAVAIGGERVADSYLDIGKLLAAAREAGADAVHPGYGLLSENAAFARAVEAAG
                     LLWVGPPPAAIEQMGDKLAARRAALAAGVEPVPGSGEPVADAAEVVAFAERHGWPVAI
                     KAAAGGGGRGIRVVRAPAAAAEALEAARREAVAAFGDGRCYLERFFERPRHVEVQILA
                     DRHGAIIHLGERDCSVQRRHQKLIEETPSPGLPAEVRERLCAWAVAVGKACGYLGAGT
                     VEFLWEPPGAAWFLEMNTRLQVEHPITEAVTGVDIVRAQLRVAAGAPLALSQELVRPR
                     GHAIEVRINAEDPARGFLPTPGPIRRLRWPGGPDLRVDAGYETGDTVPPFYDSLLGKV
                     TAWGRDRDQAIERLRAALGSLQIDGVATTAPALARILGHDDFRRAAHWTTWLEEVLAP
                     SFEDLTQRSEPDAEGPGPDFTVMVGGRSWPVRLFRHRPADLRGRRGGGRGGAGAADGG
                     AVVSPLAGTLVRVTVSPGDQVAAGDLLAVVEAMKMETPLPAPFAATVAAVPSNAGDPV
                     SAGQVVVVLEPAPEPTSG"
     gene            complement(8411..9982)
                     /locus_tag="VF995_11290"
     CDS             complement(8411..9982)
                     /locus_tag="VF995_11290"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX9344184.1"
                     /translation="MTDPKAPREALDAGERPEPAFGALAKQAEAGGAERYHAKAREQG
                     KLFVRERLRRLLDSADGFAEDGLLANAMAGDLPADGVVTGIGTIGGRTVAVMANDSTV
                     KAGSWGARTVEKIIRIEEYALRHEVPLVWLVDSAGARITDQVEMFPGRRGAGKIFYNQ
                     VRLSGRVPQVCCLFGPSAAGGAYIPAFCDVVFMVDGNASMYLGSPRMAEMVIGERTTL
                     EEMGGAAMHCRVSGCGDLLCADEDEAIQAARRWLGYFPSNWRGEPPAEPAKGPAYSGP
                     VAPLVPLDESEPFDMLPLVAAVCDEGSFFEVKREFAAELICGLARIEGRVVGVLANQP
                     LAKGGVLFVDSADKAARFIWLCDAFNIPLLFLADVPGFMIGKQVERQGIIRHGAKMIT
                     AVSEATVPKLCVVVRKAYGAGLYAMAGPGFEPDATLALPTASIAVMGPRAAVNAVYYN
                     KLAALPEADRPAEVARLEHEYREDVDLVRLASDLVVDAVVDPERLREEVARRLAAAAG
                     KDREFSRRRHGVPPV"
     gene            complement(9986..>10334)
                     /locus_tag="VF995_11295"
     CDS             complement(9986..>10334)
                     /locus_tag="VF995_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018722526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344185.1"
                     /translation="AAAGIALETSVNCHFHDTRGVGLANLLAAILHGCRDFDTAVGGL
                     GGSPTAPGAGGNVSSEDAVHMLEDMGVATGIDLGRLVEAARFAGELVGHPLRSQVLVA
                     GPRIADPGSRKGA"
BASE COUNT         1427 a         3738 c         3814 g         1355 t
ORIGIN      
        1 ccgggttcca tgccgtggcg gcgcatcagg tccagttcgg tgtcgcggtc gggcggggtg
       61 tcgccggacc acagcgcctg cagctcgcgg aagtactggt ccatcgcggg ggcgtggagc
      121 accaggaccc gcgccgctcc cgggccgggg ttgccgaagg tgtggggcac gccaccgggc
      181 accagcacga aggtccccgg gccacgccgc accgggctgg cctccccgtc gccgaggacg
      241 aattcgacgt cgccctccag cacgaaaaac gcctcctggc aattcgtgtg ccggtgcacg
      301 ggcggcctcc gccctccggg cggcaggtcc cgctgatgca gcgaaaatgc cccaccggtg
      361 gacgccgcaa tggccttgaa gagcatcacg ctcccccgcg cccgcagcgc ctcaccctca
      421 tcgggcccga agacaatcgc ctcgcccatg gcgctcacaa tcccagggag cgggcgatga
      481 ggatgcgcat gacctcgctg gtcccctccc cgatttcgag aattttcgaa tcgcggaaga
      541 accgggcgac ggggaactca ttcatgtatc cgtagccgcc gtggacctgg accgcctcgc
      601 gggcacaggt gacggccgcc tcggaggcgt gcagcttggc gatggcggcc tccttggcga
      661 agggctggcc cgcgaccagt ttggcggctg cgtgccgcca agcgaggcgg gagacgtgcg
      721 cctgggcttc catgtcggcg atcttgaagg cgaccgcctg gaaggcggcg atcggcttgc
      781 cgaaggcgcg gcgctcgcgc gcgtacttca cgcactcgtc ggcgcacccc tgggagagcc
      841 cggtggcgag cgccgcgatg gcgatccgcc cctcgtcgag ggtgcgcagg aagttggcga
      901 gcccctgccc ccgttcgccg agcaggtggc cggcggggac gcggcagtcc tcgaagacca
      961 gctcgcgggt gtcggaggcg tgccagccga ccttggagta cgccttgccg acggtgtagc
     1021 cggggatgcc ggtctcgacc acgatgttgg agatctcgcc ccgcccgtcg gcccgctcgc
     1081 cggtgacggc ggcgacggtg accaggcggg cgcgcggcag gccggcgttg gtgatgaacg
     1141 ccttggtgcc gtcgatcacc cactgcccgg cgtccgcatc gaagatggcg cgggtccgca
     1201 gcgccccggc gtccgacccg ccccccggtt cggtgaggcc gaacgcgccg atcccctcgc
     1261 cgctggcgag cggccgcagc cagcgccgct tctgctcctc ggtcccgtgc cgccagatcg
     1321 gcatcgcccc aaggccaacc gccgcctcca gcgtgagcgc caccgaggag tccacccgcg
     1381 ccagttcctc aaccgcgatg cagaaggtga cgtagtcggt ccccatcccc ccgtactcct
     1441 cgggaaacgg caacccgaac agccccatcc ccgccatctg ttcgagcacc gcctcgtcga
     1501 gccgctcctc ccggtccatc gccgcggccc tcggcgccac cacctcctcc gcgaaggacc
     1561 gaacgatctt ccgaaactcc tcctgctccg gctccaactc aaaccccatg gccaagctcc
     1621 tcgtgggcgg caccattgcc atcaggcacc tcgtgggcac ctcaccccaa tgctaggagt
     1681 ttccagctcc catcgcgggc caggcgcgac accgtaacca gcgcctcgcc cctgatcgcc
     1741 acgccattgg gatgcatctc cacctacctg gcgagagcgc ctccggtctc gtagaccgac
     1801 gcagccacta ggcgtatcag gagttgatac gttattcgta gttgatacgt tattcgatgc
     1861 gcatgagtgg gatcagcctg atccagaacg ccctctacct ccaggcacgt accctggcct
     1921 cgactacccc atcgcctcta tctcgatcct acttctggta ggatcaggcc atgggtggag
     1981 cgcagcgcac gaacaagatg agcattaccg tgccgtccga ggttgcggcg gagctgcgtg
     2041 ccagggctgg tcatggcaac gtctccgcgt acgtgaccca tgcgctcctc cggcagctcg
     2101 aacacgaccg tcttggtgat ctcgtggcgg aactcgaaaa gctgcatggg cctctgagcg
     2161 acgaagagct ggcggcggcg cgggcggaat ggcccaagga gtgagcggcc gcagacgggt
     2221 ccgctcgcgc gcgcacggat cgctggtgct ggataacgaa ggggtgtcgc gggcagcgac
     2281 cgatcgggtc atgtacgcgc ggctgcgggc cgcgcatctc gacggacgac gggtggtcac
     2341 ctccgccgcg atcctcgccg agatcctacg gggaggggcg cgtgatgcgg ggatctaccg
     2401 cgtcctcagc ggcgtggcgg tcgagccggt cacccgcgaa atcggtgagc gggcgggcaa
     2461 cctgatcggt acggccagac tcggcaccga gcaggcggtc gacgcgatgg tcgccgcgac
     2521 cgccctggag caggccgaac cggggctcct cgtcacctcc gacgttccgc acctgtcggc
     2581 gctagtcgcc gaacatgagc gggtcgccgt ggtccatgcc gacaagaccg gttgaacgac
     2641 cacgcgaccc cgtgcgccgc accgcggcta cccgccgggg cagtactcca ggccgaggag
     2701 ggcgaggtcg tcggcgtagc ggccgtcggc gtggtcggcg accagcgcga ccaggctgtc
     2761 caggacggct tgcaggtcgc tctgctcggc gctgacggcg gcgtcgaggc gggcgcggtc
     2821 gatgaagcgg ccggcgtggt cgcgggcttc gaccaggccg tcggtgtagc agatgaggcg
     2881 ctggccgggg cggagcaggc cgtaggcgtc cagggggtca ctgccgaggc cgaggggggc
     2941 ggcggcgcgg ggaaccagcg gcgggccggc gccgaggggg gcggggtggc cggcggaggc
     3001 gagcacgacg cggccgttct ggtagaggcg gccggcgagg gcggtgacga agtcctcgtc
     3061 gccgccttcg cgggcgacca ggcggtcgag gcgggcgagg gtgcgggccg ggccggcgcc
     3121 ctcgccggcg agcgtgcgga aggcgccctt gagcacggcg gccatggagc tggcctcgac
     3181 gcccttgccg cgggcgtcgg ccaggatgaa ggcgaggccc ttgccgtcga cctcggcgac
     3241 gtcgtagaag tcgccgccga tctgggcgag gccgctggcg gactggtagc gcacgccgat
     3301 gcggtacccg cccgcccacg ccggcggggt ggtcaacagc agcgcctgga gctgccgcag
     3361 caggtccgcc tgcgccttga cccgctcgga gcccgagatg gcgcgggcgc cgagaccggc
     3421 gaagcggcgc gacacctcca ggtcatgggc ggacaggcgc gggcggcggt ggcgccaggc
     3481 gagcaggatg gtgccgatct ggcgcccttc cgggtggacc agcggcagcc acagcagccc
     3541 ggcgaaccct cgcgcctgca gcatgcggat ctcggacgcc cgctgacgcg gcggcacggc
     3601 cggcagcgcc ggcggcccga acgcctgcag cggctcctcg gagaaggcgt ccgggaactc
     3661 gtcggcgctg agctggtcgg gcagcggcgg ggccaggtcg gcgcgatcct gcgcgacggc
     3721 cagcagcagc gagccctggc gccggtacac cgcgaccgcg ctggccgcgt acaggctgcg
     3781 agccgagcgg gcgagcagcc ggtaggcctg gccggtctcc aacccggcgg cgagcgaggc
     3841 ggcgatcgtg atggtgtcgg cggcgcgccc gagcgtggat cgctcgcgcc ggtgcgcgcc
     3901 ctggttgacc agcgcgatcg ggatcgcggc ggcgatcagc agcagcctga tcccgagctg
     3961 ggtgtcctgg cccggatggt ggaactcgcc gagcagcagc gcgacggcca gcgccaccag
     4021 gccgctggcg agaatccccg cgcgtccggc ctcaagccgg ctggcgagca gcggcgcgag
     4081 caccaggaac gccccggtcg acagcccgtt ccggagcagc aggtcgctca ccgcgaccgc
     4141 ggcgatcagc aggtacggcg gcagccacag ctgcaccacc gcgtcccgca gcgccgcccg
     4201 cctgagccgg cccgtcgcct ccaccagcac cccgtgtcct ccagttcaca acatcacgcg
     4261 gtaaccctga ccgatcgggc aggcaaggac aagacagcag agcaggggat tcgagcgatc
     4321 tgaagatccc ggccgcgttc cctgctcagg caactgggcg ccgggtgtca ggtggccgca
     4381 agctggggac ggctaggatg gcgttcctgg cagcgactcc aacgacggaa gggatcagat
     4441 cgggtgcgca ccctggtcac cggcggtgcc ggattcattg gctcgaccct ggtggaccgc
     4501 ctggccggcg gcggccacga ggtggtggtg gtcgacaacc tgtcgagcgg gcgggtcgag
     4561 aacctggccg acgcgctcgc ctcgggccgc gtccacctcg ccgaagtgga catcaccggc
     4621 ccggacctgg agaaggtggt cgccgaagcg cgtcccgagg tggtctatca cctcgccgcc
     4681 cagatcgacg tgcgccgcag cgtcgccgac ccggtgttcg acgctcgcgt caacgtgctc
     4741 ggcacggtcg cgctggctcg cgccaccctg gacgccgact gccgccggct ggtgttcgcc
     4801 agctccggcg ggaccgtcta cggcgagccg gacccggcgt cgctgccggt cgacgagcgc
     4861 cacccggcgc tggcgaccag tccgtatggg gtgtccaagc ggaccgccga ggactacctg
     4921 gcgagcttcg cgccgctcta cggcctggag ccggtgagcc tgcgcctcgg caacgtctac
     4981 ggcccgcgcc aggacccgca cggcgaggcg ggggtggtgg cgatcttctg caaccgcctg
     5041 ctgtcggatg agccggtgac gatcttcggc gacggcctgc agacccgcga ctacgtgttc
     5101 gtcgaggacg tggtcgacgc gttgctggca gccggcgagc gtgacgaggc agtcggcgcc
     5161 cgcgtcaaca tcggcaccgg catcgagacc tcggtcctgg acctgtacca ggccctccgg
     5221 gaggtcaccg gcttcggccc ggaaccccag ttggcgcccc ctcgccccgg cgagttgcag
     5281 cgtatcgccc tcgattgcgg agcggccgag cggacgctcc gctggcaacc agcaacaacg
     5341 ctcaccgagg gcctctcccg tacctgggat tgggcgtttc agcaggtcaa cgccggtagc
     5401 gtcggcggat gaaccccatc ggggtcgagg tcgcggaggg gatcgcgacc gtcacgctcc
     5461 agcggccggg ggcgctcaac gcgatctcga ccgagctggc gctggcgctg gccgaggcgg
     5521 tcgagccgct ggcgaccaag ccgtcggtgc gggcgctggt gctgcggggc gcgggcgagc
     5581 gggccttctg cgtcggcgcg gacctgcggg agcggggcgg gttcgacgat catgcgtggt
     5641 tcgtgcagcg agaggtgttc cggcgcgggt tcggggcgct gcggcgctgt ccgctgccga
     5701 cggtcgcggc gctgttcggg ttcgcgctcg gcggtgggat ggagctggcg ctcgcctgcg
     5761 acctgatcgt cgccgccgac gacgcggtct tcggcctgcc ggaggtgcgg ctcgggctgg
     5821 ttcccgccgg tgggggcacc cagctgctgg tccgccgggt ggggcggtcg gccgccaagg
     5881 acctggtgct caccggcagg cgggtggacg cggccgaggc gctgcggctc ggcatcgccg
     5941 accgggtggt gccccgcgcg gagctgccgg acgcggcccg ggcgctggcc gccgagatcg
     6001 ccgccaacgc ccccaccgcc gtccgcctcg ccaaatgggc gcttgacctc ggcgccgacc
     6061 tcgcccagga ggccgccatc gaggtcgagg accaggcgtg gcgccgcgcg gtcctctccg
     6121 gcgaccgccg cgagggcatc gccgcctgga acgaacgccg cgacccgcga tggccggcca
     6181 gttgaacacc ctgctctgga tgcggcgccg cccagcggca acgcgacgat cagcgatcag
     6241 ccagcggcgg cgagttggga gcggagctgg gcgaggtagg tgcggcggta gcgtttgacc
     6301 tcgtcggggg cttcgaagag ctggtttcgg gaggcggcgg cccagcggtc gaactggtcg
     6361 aagagctgct gcagctgggt ggtggcgtcc agggcggcgg ggacctgggc ctcggggatc
     6421 accacggaag cctggaatgc tggctcgccg gccaggaagg cccggtcggc ggcctcgtcg
     6481 acggtgtctt cggggacctg ggcgcggatg cggtcgagca gttcgccgaa ccgtctcggg
     6541 acgctgtcgg cggccagttc gaggagctgg atctcgcggc ggaccgcctc gtaatggtca
     6601 cggctgcgga catggagcgc ggggtcgaac ggacctagcg tgatttccac ggccacccct
     6661 cacccgctcg ttggctccgg cgctggctca agcacgacga cgacctggcc ggcggagacc
     6721 ggatctccag cgttcgaggg taccgccgcc acggtggccg cgaagggggc cggcagcggc
     6781 gtctccatct tcatcgcctc gacgaccgcg agcagatccc cggcggccac ctggtcaccg
     6841 gggctgaccg tgacccggac cagggtccca gccaggggcg agacgacagc cccgccgtcg
     6901 gcggccccgg caccaccccg gcctccgcct cggcggccac ggaggtccgc gggccggtgc
     6961 cggaacaggc gcaccggcca ggaccggccc cccaccatga cggtgaagtc gggaccgggc
     7021 ccctcggcgt cgggctcgga ccgctgggtg agatcctcaa aagagggggc gagcacctcc
     7081 tccagccagg tcgtccagtg cgccgcccgg cggaagtcgt catggccgag gatccgggcg
     7141 agcgcggggg cggtggtggc caccccgtcg atttggaggc tgccgagggc ggcgcgcagg
     7201 cgctcgatcg cctggtcgcg gtcgcggccc caggcggtga ccttgccgag gaggctgtcg
     7261 tagaacggcg gcacggtgtc gccggtctcg tagccggcgt cgacgcggag gtccgggccc
     7321 cctggccacc ggagccggcg gatcggtccg ggggtgggca ggaagccccg ggcggggtcc
     7381 tcggcgttga tgcggacctc gatcgcgtgg ccgcgtgggc gcaccagctc ctgggagagc
     7441 gccagcgggg cgccggccgc gacgcggagc tgcgcccgga cgatgtcgac gccggtgacc
     7501 gcctcggtga tcgggtgctc gacctggagg cgggtgttca tctccaggaa ccaggcagcg
     7561 ccgggaggtt cccagaggaa ctcgacggtg cccgcgccga ggtagccgca ggccttgccg
     7621 accgcgaccg cccaggcgca gagccgctcg cggacctcgg cgggcaggcc cggcgatggg
     7681 gtctcctcga tcagcttctg gtggcggcgc tgcaccgagc agtcacgctc gccgaggtgg
     7741 atgatcgcgc cgtggcggtc ggcgagaatc tgcacctcga cgtggcgggg ccgttcgaag
     7801 aagcgttcca ggtagcagcg gccgtccccg aaggcggcga cggcctcacg gcgcgcggcc
     7861 tccagcgcct cggccgccgc ggcgggcgcg cggaccacgc ggatgccacg cccgcccccg
     7921 ccggcggcgg ccttgatcgc caccggccag ccgtgccgct cggcgaaggc gaccacctcg
     7981 gcggcgtcgg cgaccggctc gccgctgccc ggcaccggtt cgactccggc ggcgagcgcc
     8041 gcgcggcggg ccgccagctt gtcccccatc tgctcgatcg cggcgggagg gggcccgacc
     8101 cagagcagcc cggccgcctc aaccgcgcgg gcgaacgccg cgttctcgct gagcagccca
     8161 tacccggggt ggacggcgtc cgcgccggcc tcccgcgcgg cggcgagcag cttgccgatg
     8221 tcgaggtagg agtcggcgac ccgctccccg ccgatcgcga cagcgcggtc ggcctcgcgg
     8281 acgtgcagcg cgcccgcgtc gggttcggcg tagaccgcga tccccgccag gccctcctcc
     8341 cggcaggcgc gcagcacccg cacggcgatc tcgccccggt tggcgaccag caccgcccgc
     8401 atcccggcct tcataccggc gggaccccgt ggcgccgccg gctgaactcg cggtccttgc
     8461 cggcggcggc ggccaggcgg cgggcgacct cctcccgcag ccgctcggga tcgacgaccg
     8521 cgtcgaccac caggtcggag gcgaggcgga ccaggtcgac gtcctcgcgg tactcgtgct
     8581 caaggcgggc gacctcggcg gggcggtcgg cctcggggag ggcggcgagc ttgttgtagt
     8641 agaccgcgtt gacggccgcc cgcgggccca tcaccgcgat cgaggcggtc ggcagcgcca
     8701 gggtggcgtc cggctcgaag ccgggcccgg ccatggcgta cagcccggcg ccgtacgcct
     8761 tgcggaccac cacgcagagt ttcgggacgg tcgcctcact gacggcggtg atcatcttgg
     8821 cgccgtggcg gatgatcccc tggcgttcga cctgcttgcc gatcatgaag ccgggcacgt
     8881 cggcgaggaa cagcagcggg atgttgaacg cgtcgcacag ccagatgaag cgggcggcct
     8941 tgtcggccga gtcgacgaac agcaccccgc ccttggccag cggctggttg gcgagcaccc
     9001 cgacgacccg gccctcgatc cgcgccagcc cgcagatcag ctccgcggcg aactcgcgct
     9061 tgacctcgaa gaagctgccc tcgtcgcaga ccgcggcgac cagcgggagc atgtcgaacg
     9121 gttcggactc gtccagcggg accaaggggg ccaccgggcc gctgtaggcg gggcccttgg
     9181 cgggctcggc gggaggctcc ccgcgccaat tcgacgggaa gtagccgagc cagcggcgcg
     9241 cggcctggat cgcctcgtcc tcgtcggcgc agagcaggtc cccgcagccg gacacgcggc
     9301 agtgcatcgc cgcgccgccc atctcctcca gggtggtccg ctcgccgatc accatctccg
     9361 ccatccgcgg cgagccgagg tacatcgagg cgttgccgtc caccatgaag accacgtcgc
     9421 agaaggcggg gatgtaggcc ccgcccgccg ccgacggccc gaacaggcag cacacctgcg
     9481 gcacccgccc ggacaggcgg acctggttgt agaagatctt cccggccccc cgccgcccgg
     9541 ggaacatctc cacctggtcg gtgatccggg ccccggccga gtccaccagc cagaccagcg
     9601 gcacctcgtg gcgcagggcg tattcctcga tgcggatgat cttctcgacg gtgcgcgctc
     9661 cccaggagcc cgccttgacg gtggagtcgt tggccatcac cgcgacggtg cgcccgccga
     9721 tcgtgccgat cccggtgacc accccgtcgg cgggcaggtc gccggccatg gcgttggcca
     9781 gcaggccgtc ctcggcgaag ccgtcggcgc tgtcgagcag ccgccgcagc cgctcccgca
     9841 cgaacagctt gccctgctcc cgcgccttgg cgtggtagcg ctccgccccg cccgcctcgg
     9901 cctgcttggc gagcgccccg aacgccggct cgggccgctc cccagcgtca agcgcctccc
     9961 ggggcgcctt cgggtcggtc accgttcagg cccccttcct gctacccggg tccgcgatgc
    10021 gaggcccggc gaccagcacc tggctccgca gcggatggcc gaccagctcg ccggcgaacc
    10081 gggccgcctc caccagccgg cccagatcga tgccggtggc gacgcccatg tcctccagca
    10141 tgtggacggc gtcctcgctg gagacgttcc cgccagctcc cggcgccgtc gggctgccgc
    10201 caagcccgcc gaccgccgtg tcgaagtcgc ggcacccgtg caggatcgcc gccagcagat
    10261 tcgcgagccc caccccccgg gtatcgtgga aatggcagtt caccgaggtc tccagcgcga
    10321 tcccagcggc agcg
//
LOCUS       DASWDC010000352         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_487008, whole genome shotgun sequence.
ACCESSION   DASWDC010000352 DASWDC010000000
VERSION     DASWDC010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487008"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VF995_11300"
     CDS             complement(<1..903)
                     /locus_tag="VF995_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344186.1"
                     /translation="MPVATASPTVFALLDAEWATLARAPIPLRWRHHPALALPDAAGR
                     TSTLSDLLQITERRDDPAASDQILAALAQLTRGNPDDDDTHAPLAARTLLQTLLPGAK
                     ALARRLTWLGDPPERAAAVTATLYEQIRSYPIQRRPARIAANLLADTHQRLLRHASAS
                     ASASGSGSGAPHRRQGCGGGATMALPVVSLDELAAHGDAMLPAPAPSQRQPSPAEELL
                     ALLAWAVHGGWLTPAQATLIGQSRIADIPCEELGAQAGLGAHSLRRRRQRAEQALGRA
                     ATASALSPEELARLAPQLACRVPAT"
     gene            1205..>1633
                     /locus_tag="VF995_11305"
     CDS             1205..>1633
                     /locus_tag="VF995_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016908305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX9344187.1"
                     /translation="MAGNSFGARSGLEVGGRSYEIFRLDALAQVADVGRLPFSLKVLL
                     ENLLRNEDGSVVTADDIEGFARWDPSATPSQEIAFAPARVLLQDLTGVPAVVDLAVMR
                     DAMAEQGGDVGKINPLVPAELVVDHSVIADFAGMREAFARN"
BASE COUNT          188 a          501 c          701 g          243 t
ORIGIN      
        1 cgtggccggg acgcgacacg ccagttgcgg cgccagccgc gcaagttcct ccggggagag
       61 ggcggacgcg gtcgccgcgc gtccgagggc ctgctcggcg cgctggcggc ggcgccgcag
      121 gctgtgggcg ccgagcccgg cctgggcgcc gagttcttcg caggggatgt cggcgatgcg
      181 gctctggccg atcagggtgg cctgggcggg ggtgagccag ccgccgtgga ccgcccaggc
      241 cagcagcgcc agcagctcct cggcggggga gggctggcgc tggctggggg ctggtgcggg
      301 cagcatggcg tcgccgtggg cggccagctc gtccagtgac accaccggca gggccatggt
      361 ggcgccgccg ccgcacccct ggcggcggtg cggggcgccg ctgccgctgc cgctggcgct
      421 ggcgctggcg ctggcgtggc gcagcagccg ctggtgggtg tcggcgagca ggttggcggc
      481 gatgcgggcg gggcggcgtt ggatggggta gctgcggatc tgttcgtaga gggtggcggt
      541 gacggcggcg gcgcgttcgg gggggtcgcc gagccaggtg aggcggcggg cgagggcttt
      601 ggcgccgggg agcagcgtct gcagcagtgt gcgggcggcc agcggggcat gggtgtcgtc
      661 gtcgtcgggg ttgccgcggg tgagctgggc gagggcggcg aggatctggt cgctggcggc
      721 ggggtcgtcg cggcgttcgg tgatctggag caggtcgctg agggtgctgg tgcggccagc
      781 ggcgtcgggg agggccaggg cggggtggtg gcgccagcgg agggggatgg gagcgcgggc
      841 cagggtggcc cattcggcgt ccagcagcgc gaacaccgtg ggggaggcgg tggcgaccgg
      901 catgggctgg ggtcctttcg ggcgggggcg gttgccctca ccctcagacg caccactttc
      961 gggaaatctc acacccggcc tgtcagccgg tgactttctt cggccaattg cggtccggct
     1021 gcgtggccgg tggcgctgac aggcgcccag taagccagct gaccagcgga tacgccgctc
     1081 tgagccgtca caggcggggc tgtggataag ctccgacagg ggtctgtcca ggtgggtacc
     1141 cttggtggcg ttgagatcgg cgtcaccgcg cagcggcgcg cggcctgggt ggaggttgac
     1201 tgacatggca ggtaacagct tcggagcacg gtcaggcctt gaagtcgggg ggcgttccta
     1261 cgagatcttc cggctcgacg ccctggcgca ggtcgccgac gtcggtcggc tgccgttctc
     1321 gctcaaggtg ctgctggaaa atctgctccg caacgaggac ggcagcgtcg tcacggccga
     1381 cgatatcgag gggttcgccc gctgggaccc ctcagccacc ccgagtcagg agatcgcctt
     1441 cgcgcccgcg cgggtgctgc tgcaggacct caccggcgtg ccggcggtgg tcgacctcgc
     1501 ggtgatgcgg gacgccatgg ccgagcaggg cggcgacgtc ggcaagatca acccgctggt
     1561 ccccgccgag ctggtggtcg accactcggt gatcgccgac ttcgccggca tgcgcgaggc
     1621 gttcgcccgc aac
//
LOCUS       DASWDC010000353         7870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_487172, whole genome shotgun sequence.
ACCESSION   DASWDC010000353 DASWDC010000000
VERSION     DASWDC010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487172"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VF995_11310"
     CDS             <1..657
                     /locus_tag="VF995_11310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629368.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9344188.1"
                     /translation="FVQAGSEVSTLLGRMPSAVGYQPTLADEMGELQERITSTRGHSI
                     TSLQAIYVPADDITDPAPHTTFTHLDATTVLAREIASLGIYPAVDPLNSTSRILDARY
                     VGEEHYQVAQRVKEILQRYNDLQDIIAILGIDELSEDDKVTVNRARKIQRFLSQPFYV
                     AEQFTGVKGKTVPVDETVASFKAVAEGAYDHLPEQAFFNVGGIEEVEEKAKRIEQGAA
                     "
     gene            657..1058
                     /gene="atpC"
                     /locus_tag="VF995_11315"
     CDS             657..1058
                     /gene="atpC"
                     /locus_tag="VF995_11315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX9344189.1"
                     /translation="MPSLDLHLVTPEREVWSGTASYVVVRSVDGDLGVLPEHAPTLAA
                     LDVGPVYIEAEGGRTAVVVDGGFLHVGPVDDGVTRVDILAEHAVLADELDREDPAACD
                     ARAEELEAEGRHAEARVERAKAAARRRLAER"
     gene            1217..2989
                     /locus_tag="VF995_11320"
     CDS             1217..2989
                     /locus_tag="VF995_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX9344190.1"
                     /translation="MIEQGVEELPQAFPEELPPLVTVPETGRSGLAEALLAVATALNQ
                     GRGRQEVLERIAAELQRLVPHTGIMIGRADQRTSTVSLVFGRGENMAKMLGMQVGYGE
                     GLCGRVAETGMPAVWNRSADDDPTLKPKIAPGCPPDPKQYVMVVPLCGNGRVEGTLAL
                     YREGDGQPRWTDKDLELAQLFATQAQVAFHNAELYDEVQERARRLQAMNELLRPTSAR
                     LGLGQICRSYEPALRQLFPFTAAGICVRQRPGKLTQVWASDGVGWKAGTTLPAGNSAW
                     WVIEHGRGYVVDDLDSEHGYGRHCGFQEAGAVSFVTAPLKARGRIFGVLGLGHSDRGA
                     YDQHSLKLLEELAVYVAASLENAMLYQEVRAAKKDQSQLLAKLITAQEEERKALAADL
                     HDDTIQSFAAGLMLVDRIIGADQRERRLDLTGKLRHTLQSAMDRARKLMVDLRPPVLD
                     SEGLAAALDQQLKLLRTEDGMVTELTSTLDGRLGATVEILLFRSFQEALQNIRKHARA
                     SRVAVALTCGPEEGWVTLTISDDGVGFDPPAVLPKAVAAGHLGLHSLLQRVELAGGRT
                     EVCAGPNAGTRLVVSVPSALGGAE"
     gene            2991..3359
                     /locus_tag="VF995_11325"
     CDS             2991..3359
                     /locus_tag="VF995_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9344191.1"
                     /translation="MIRVLVVDDNDMLRQTLAEALDDRDIQVVGQATDGEQACWAAQD
                     LRPDVVVMDVRMPGMSGPEATAWLSKQFPEVRVVALTAYEDAALHQAMTRAGAVSVLV
                     KGTSLRDIVGAIHDAVAVPG"
     gene            complement(3362..4105)
                     /locus_tag="VF995_11330"
     CDS             complement(3362..4105)
                     /locus_tag="VF995_11330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX9344192.1"
                     /translation="MATLLVVEHEEGCPPGLLGDAARDAGVTLWRVAAGAGERLPVDG
                     ELAGVDGVVVLGGSMDVGDLPRYPHLGEVMGLIRRAAERELPTLGICLGSQLAAQALG
                     GRAHRGEAGLEVGWTALALTPAGRADPVLGALDGALHHDDGESDRNNGARVFHWHHDT
                     YEPPPGATLLARGTQYPNQAFRLGSVVAVQFHPEVDAHLIRRWYAESSELPPVPLEVS
                     VAGAARYAATARRVLDAFCATVRPAETAP"
     gene            4176..6086
                     /locus_tag="VF995_11335"
     CDS             4176..6086
                     /locus_tag="VF995_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344193.1"
                     /translation="MRIIRGVGALALAALLTCASGFGAPARAQASSAPGRPVALLLFE
                     RLSWAEAAGAAAAAEQGGATVSAGLVSTLPADASLAARVASVLAGREVTRAGPVTDST
                     VVGGVPVQLVRDTEATHNQDELFVLNTLVEVLAKPRSGPLLVAGLVAPPGHARVAPFL
                     ELGSGGPAGVATSDSTRQRGLVAFQDLRATLTGTATDTDGAPIRVLADPHALQTISHL
                     DHQAATLVATRTLAIPIFATLGTVAVAAALAALLLAWRWPLTHRGAPRQPGAQASMVP
                     AAGPSPPNPPLPGWPGRARTLARVLLLLTFAVPAGYLLSSIVAPSSAAAWLTLGLATA
                     AAFALAAAAAATAGRAPPGPGRAWDRAPALLGALLAALVVVDLLAGGHALGRPLLGNS
                     IFDGERFYGLGNGYFANAFAGLLLLVAFAPVPALPSAGLFAAFAVIDGLPMLGADVGG
                     ALTAMVTAALALLLLSGRRPSARAVVAWVGLAVAAAVAVALGAALLDRQATHGSRFAH
                     DLLHNPGAATRALGSQLHGNLPLLAGNFWAWWGPLLVLSAGLASRWPTPLLARVPQWV
                     RQVALVGALGSAVLIVLNDTGVTAAAAPGLFLIGALGWSGLESAKPGESPTVSGRAAR
                     YALWLWHTLSAP"
     gene            6128..6976
                     /locus_tag="VF995_11340"
     CDS             6128..6976
                     /locus_tag="VF995_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9344194.1"
                     /translation="MADKVLVVGAGLMGAGIAQVAAVAGYEVTLQDVSEAALARGRRQ
                     LDQSLARLETKGKLTPQDRAAALQRIAATTELEAASGAEIVVEAVFEELEVKREVFRR
                     LDKLAPEGAMLATNTSAIPITSIAAATKRPESVVGTHFFSPVPMMGLCELVRGRRTAD
                     ATLERAQRFATACGKTTVVVERDIAGFVTTRLITAVAMEAARLVEQGVISAEDLDTAC
                     RLGFGWAMGPIATIDLTGVDVLYHAALNIYNDTRDPKFFPPESLARMVEAGDLGRKTG
                     RGFYGY"
     gene            6992..>7870
                     /locus_tag="VF995_11345"
     CDS             6992..>7870
                     /locus_tag="VF995_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX9344195.1"
                     /translation="MPTPAPTSPRRLNGTLPWVWAVAVTAVVLLLGAAVMVLPKLLIP
                     GSVTPQQADALKAQDDLRTILVQSVGGLLLVGGVVATWRQVTLGRRQLEIMREGQLTE
                     RFSRAIEQLGSDKLDVRLGAIYALERIAVASADERGPILEVLTAYVRGHSPWPPSGWD
                     YGTGTPSDDEEADRPMRSLQFRAPDVAAVVIILGRRPASWTEPEELLLSRVDLRQAYL
                     RRANFDRFNLRNANLRRVQARGAHLRAAKLKYANLAEARLDGADLRDAELVGANLRGA
                     DLRLAKLGGADLRGADL"
BASE COUNT         1049 a         2983 c         2776 g         1062 t
ORIGIN      
        1 ttcgtccagg ccggctccga ggtctccacc ctgctcggcc ggatgccgtc ggcggtgggc
       61 taccagccga ccctggccga cgagatgggt gagctgcagg agcggatcac ctcgacgcgg
      121 ggccattcga tcacctcgct gcaggcgatc tacgtgcccg ccgacgacat caccgacccg
      181 gcgccgcaca ccaccttcac ccacctggac gccaccacgg tgctggcccg cgagatcgcc
      241 tcactgggca tctacccggc ggtggacccg ctcaactcga cctcgcggat cctggacgcc
      301 cgctatgtcg gcgaggagca ctatcaggtc gcccagcggg tcaaggagat cctgcagcgc
      361 tacaacgacc tgcaggacat catcgccatc ctcggcatcg acgagctgtc cgaggacgac
      421 aaggtgaccg tcaaccgcgc ccgcaagatc cagcgcttcc tgtcgcagcc gttctatgtc
      481 gccgagcagt tcaccggtgt caagggcaag accgtcccgg tcgacgagac ggtcgcctcc
      541 ttcaaggcgg tcgctgaagg cgcctacgac cacctgcccg agcaggcgtt cttcaacgtc
      601 ggcgggatcg aagaggtcga ggagaaggcc aagcggatcg agcagggggc cgcgtagtgc
      661 cgtccctgga cctgcacctg gtcacccccg agcgggaggt gtggagcggc accgccagct
      721 acgtggtggt tcgctcggtc gacggcgacc tcggggtgct acccgagcac gccccgaccc
      781 tcgccgcgct ggacgtcggg ccggtctaca tcgaggccga ggggggccgc acggcagtcg
      841 tcgtcgacgg cggcttcctg cacgtcgggc cggtcgacga cggggtcacc cgggtcgaca
      901 tcctggccga gcacgcggtc ctcgccgacg agctggaccg cgaggatccc gccgcctgcg
      961 acgcccgcgc cgaggagctg gaggccgagg gccgccacgc cgaggcgcgg gtcgagcgcg
     1021 ccaaggccgc ggcccgccgc cgcctcgccg agcgctgacc cggctgacca ccgtgcgcgg
     1081 cggcttgacg cgaagggcga gcgacgcagg ttgaatcctc gattcgcgtc aaaggttggt
     1141 ttcacggtat ggtgctgcct ctggactagt cagtcttgac gatgctggcg ggtgggtatt
     1201 ggccggtgcc gagcaggtga tcgagcaagg cgtcgaggaa ctccctcagg catttccgga
     1261 ggaactccca cccttggtca ccgtccccga gacgggcagg tcgggccttg ccgaggcgct
     1321 gctggcggtg gcgaccgccc tcaaccaggg acgcgggcgc caggaagtgc tcgaacggat
     1381 cgccgccgag ctgcaacggc tggtccccca caccgggatc atgatcggcc gcgccgacca
     1441 gcgcaccagc accgtgtcgc tggtgttcgg gcgcggcgag aacatggcga agatgctcgg
     1501 catgcaggtc ggctacggcg aaggcctctg cggccgggtc gctgagacgg gcatgccggc
     1561 ggtctggaac cgctccgccg acgacgaccc gactctcaag ccgaagatcg cgccgggctg
     1621 cccgcccgat cccaagcagt acgtcatggt ggtgccgctg tgcggcaatg gccgggtcga
     1681 gggcaccctc gccctctacc gcgaaggcga cggtcagcca cgctggaccg acaaggacct
     1741 ggagctggcg cagctgttcg ccacccaggc gcaggtcgcc ttccacaacg ccgagctgta
     1801 cgacgaggtc caggagcgcg cccggcgcct gcaggcgatg aatgagctgc tgcggccgac
     1861 ctcggcgcgg ctcggcctgg gccagatctg ccgctcctac gagccggcgc tgcgccagct
     1921 gttcccgttc accgccgccg gcatctgcgt ccggcagcgg cccggcaagc tcacccaggt
     1981 ctgggcctca gacggcgtcg gctggaaggc cggcaccacc ctgccggccg gcaactctgc
     2041 ctggtgggtg atcgagcacg gccgcggcta cgtggtcgac gacctcgaca gcgagcacgg
     2101 ctacggccgc cactgcggct tccaggaggc cggcgccgtc tcgttcgtca ccgcgccgct
     2161 caaggccaga ggccgcatct tcggcgtcct cgggctcggc cactccgacc ggggcgccta
     2221 cgaccagcac agcctgaagc tgctggagga gctggccgtc tacgtcgccg cgtcgctcga
     2281 gaacgccatg ctctaccagg aggtccgcgc cgccaagaag gaccagagcc agctgctcgc
     2341 caagctgatc accgcccagg aggaggagcg caaggcgctc gccgccgacc tgcacgacga
     2401 caccatccag tcgttcgccg ccgggcttat gctggtcgac cggatcatcg gcgccgacca
     2461 gcgcgagcgg cgcctggacc tgaccggcaa gctgcgccac accctgcaga gcgcgatgga
     2521 ccgcgcccgc aagctgatgg tcgacctgcg cccgccggtg ctggacagcg agggcctggc
     2581 cgccgcgctc gaccagcagc tcaagctgct gcgcaccgag gacggcatgg tcaccgagct
     2641 gacctccacc ctcgacgggc gcctcggcgc caccgtcgag atcctgctgt tccgcagctt
     2701 ccaggaggcc ctgcagaaca tccgcaagca cgccagggcc agccgggtgg cggtggcgct
     2761 gacctgcggc ccggaagagg gctgggtcac cctgaccatc agcgacgacg gtgtcggctt
     2821 cgacccgccg gcggtcctgc cgaaggcggt cgcggccggc cacctcgggc tgcactcgct
     2881 gctgcagcgg gtcgagctgg ccggtggccg cacggaggtc tgtgccggtc ccaatgccgg
     2941 gacgcggctg gtcgtctcgg tgccgagcgc gctcggaggg gcagagtgac gtgatcagag
     3001 tgctggtggt cgacgacaac gacatgctgc gccagaccct cgccgaggcg ctcgacgacc
     3061 gcgacatcca ggtcgtcggg caggcgaccg acggcgagca ggcctgctgg gccgcgcagg
     3121 acctgcggcc cgacgtggtg gtgatggacg tccgcatgcc cggcatgtcc ggcccggagg
     3181 ccaccgcctg gctcagcaag cagttccccg aagtgcgggt ggtcgccctg accgcctacg
     3241 aggacgccgc cctccaccag gccatgaccc gcgccggggc ggtctccgtc ctggtcaagg
     3301 gcacctccct gcgcgacatc gtcggcgcca tccacgacgc cgtcgccgta ccgggctagg
     3361 gctagggagc ggtctcggcg ggacgcaccg tggcgcagaa ggcgtcgagc acccggcgcg
     3421 ccgtggcggc gtagcgggcc gcgcctgcca ccgacacctc cagcgggacg ggtgggagct
     3481 cgctggactc ggcgtaccag cgccggatca ggtgggcgtc gacctcgggg tggaactgca
     3541 ccgcgaccac gctgccgagc cggaacgcct ggttgggata ctgcgtgcca cgcgccagca
     3601 gggtcgcgcc cggcggcggc tcgtaggtgt cgtggtgcca gtggaacacc cgggcgccgt
     3661 tgttgcggtc gctctcgccg tcgtcatggt ggagggcgcc gtcgagggcg ccgagcaccg
     3721 ggtcggcccg ccccgccggg gtcagtgcca gggcggtcca gccgacctcg aggccggcct
     3781 cgccccggtg ggcgcgccca ccgagcgcct gggcggcgag ctgcgagccg aggcagatcc
     3841 caagcgttgg cagctcccgc tccgccgccc gccggatcag ccccatgacc tccccgagat
     3901 gcgggtaccg cggcaggtcc ccgacgtcca tgctgccgcc gagcaccacc acgccgtcca
     3961 cgccggcaag ctcgccgtcg accggcagcc gctcacccgc gccggccgcg acccgccaca
     4021 gcgtcacccc ggcgtcgcgg gcggcgtcgc cgagcagccc cggcgggcag ccctcctcat
     4081 gctccaccac caacagcgtt gccacgcgca cctcctgtca cctgaaggtc agcgtagcgg
     4141 ggcgtcgcgc tgccatgccg gtagaatccc gcgcgatgag gatcatccgc ggcgtcggcg
     4201 cgctggcgct ggccgctctc ctgacctgcg cctccggctt cggggcaccg gcgcgtgcgc
     4261 aggcctcctc ggcgcccggc cgcccggtcg cgctgctgct gttcgagcgg ctgtcctggg
     4321 ccgaggcggc cggggcggcg gccgccgctg agcaaggggg cgcgacggta tccgccgggc
     4381 tggtctcgac cctgccggcg gacgcctcgc tggcggcgcg ggtggcatcg gtcctcgccg
     4441 gtcgcgaggt cacgcgagct ggaccggtta cggacagcac tgtggtgggc ggtgtgccgg
     4501 tccagctggt gagggacacc gaggcgacgc acaaccaaga cgagctgttc gtgctcaaca
     4561 ccctcgtgga ggtcctcgcc aagccccgga gcgggccgct gctggtggcc ggactggtcg
     4621 cgccgcccgg ccacgcgcgg gtcgcgccgt tcctggagct tggatctgga gggccggccg
     4681 gggttgccac ctccgactcg acccggcagc gcggcctggt ggccttccag gacctccgcg
     4741 cgaccctcac cgggaccgcc accgataccg acggggcacc gatccgggtg ctcgccgacc
     4801 cgcacgccct ccagacgatc agccatctcg accatcaggc cgccaccctg gtcgccaccc
     4861 gcaccctggc gatcccgatc ttcgccacac tcggtacggt ggcggtggcc gccgccctgg
     4921 ccgccctgct gctggcttgg cgctggcctt tgacgcatcg gggggccccg cggcaaccgg
     4981 gggctcaggc ctcgatggtt ccggccgcgg gaccaagccc cccgaacccc ccgctgccag
     5041 gctggcccgg ccgcgcccgg acgctcgcca gggtgttgct gctgctcacc ttcgcggtcc
     5101 ccgccggcta cctcctgtcg agcatcgtgg cgccgtcctc ggcagccgcc tggctgaccc
     5161 tcgggctggc cacggccgcg gccttcgcgc tggccgccgc cgcggcagcc acggccggca
     5221 gggccccgcc tggaccgggc cgggcctggg accgcgcccc cgcgctgctc ggcgcgctgc
     5281 tcgccgcgct ggtcgtcgtc gacctgctgg ccggcgggca cgcgctcggc cggccgctgc
     5341 tcggcaacag catcttcgac ggcgagcggt tctacggcct cggcaacggc tacttcgcca
     5401 acgccttcgc cgggctgctg ctgctggtcg ccttcgcgcc ggtcccggcc ctgccaagcg
     5461 ccggcctgtt cgccgccttc gccgtcatcg acgggctgcc gatgctcggc gccgacgtcg
     5521 gcggtgcgct caccgccatg gtcaccgcgg cgctggccct cctgctgctc agcggcaggc
     5581 gtccgagtgc ccgcgccgtg gtcgcctggg tcgggctcgc ggtcgccgcc gccgttgcgg
     5641 tcgcgctggg cgccgcgctg ctcgaccggc aggcgaccca cggcagccgc ttcgcccacg
     5701 acctgctcca caaccctggc gccgccaccc gcgcgctcgg cagccagctc cacggcaacc
     5761 tccccctgct ggccggcaac ttctgggcgt ggtgggggcc gctgctggtg ctcagcgccg
     5821 gtctcgccag ccgctggccg accccgctgc tcgcccgcgt gccccaatgg gtccgccagg
     5881 tcgccctggt tggcgcgctc ggctcggcgg tgctgatcgt cctcaacgac accggcgtga
     5941 ccgccgcggc ggcccccggg ctgttcctga tcggcgcgct gggctggtcc ggcctggaat
     6001 ccgcgaaacc tggcgaatcg cctactgtat cgggacgcgc ggcacgatac gctctgtggc
     6061 tctggcacac gctctcggcc ccgtgatcga ggcccggggc ggtgcggtcg cggcagagga
     6121 ggcggcggtg gcggacaagg tgctggtggt cggggctggg ctgatgggcg cggggatcgc
     6181 ccaggtcgcc gccgtggcgg gctatgaggt gaccctccag gacgtgagcg aggccgccct
     6241 cgcccggggc cgccggcagc tcgaccagtc cctggcccgc ttggagacca agggcaagct
     6301 caccccgcag gaccgcgccg ccgccctgca gcgcatcgcc gccaccaccg agctggaggc
     6361 cgccagcgga gccgagatcg tcgtcgaggc ggtcttcgag gagctggagg tcaagcggga
     6421 ggtgttccgc cgcctcgaca agctcgcgcc cgagggtgcc atgctcgcca ccaacaccag
     6481 cgcgatcccg atcacctcga tcgccgccgc caccaaacgg cccgagtcgg tcgtcggcac
     6541 gcacttcttc tccccggtcc cgatgatggg cctgtgcgag ctggtccgcg ggcgccgcac
     6601 cgccgacgcg accctggagc gggcccagcg cttcgccacc gcctgcggca agaccaccgt
     6661 ggtggtcgag cgcgacatcg ccgggttcgt caccacccgc ctgatcaccg ccgtcgcgat
     6721 ggaggccgcc cgcctggtcg agcagggcgt gatcagcgcc gaggacctgg acaccgcctg
     6781 ccgcctcggc ttcggctggg cgatgggccc gatcgccacc atcgacctga ccggcgtgga
     6841 cgtcctctac cacgccgctc tgaacatcta caacgacacc cgcgacccga agttcttccc
     6901 cccggagagc ttggcccgca tggtcgaggc cggcgacctc ggccgcaaga ccggccgggg
     6961 cttctacggc tactgagccg gggagccgcc gatgccgacg ccagcgccga cgtcaccgcg
     7021 ccgcctgaac ggcacccttc cctgggtctg ggccgtcgcc gtcaccgccg tcgtgctgct
     7081 gctcggcgcg gcggtgatgg tgctgcccaa gctgctgatc ccgggcagcg tcaccccgca
     7141 gcaggccgac gccctcaagg cccaggacga cctgcgcacc atcctggtgc agagcgtcgg
     7201 cgggctgctg ctggtcggcg gcgtggtggc gacctggcgg caggtcaccc tcggccgccg
     7261 ccagctggag atcatgcgcg aaggccagct caccgagcgc ttcagccgcg ccatcgagca
     7321 gcttggcagc gacaagctcg acgtccgcct cggcgccatc tacgcgctgg agcggatcgc
     7381 cgtcgcctcc gccgacgagc gcggcccgat cctggaggtg ctgaccgcct acgtccgcgg
     7441 ccactccccc tggcccccgt ccggctggga ctacggcacc ggcacgccct ccgacgacga
     7501 ggaggccgac cggccgatgc gctcgctgca gttccgcgcc cccgacgtgg cggcggtcgt
     7561 catcatcctc gggcggcgcc cggcgagctg gaccgagccg gaggagctgc tgctgtcgcg
     7621 ggtcgacctg cgccaggcct acctgcggcg tgccaacttc gaccggttca acctccgcaa
     7681 cgcgaacctg cgccgcgtgc aggcgcgggg cgcccacctt cgtgccgcca agctcaagta
     7741 cgcgaacctc gcggaggccc ggctggacgg tgccgacctg cgcgatgccg agttggtcgg
     7801 cgccaacctg cgcggcgcgg acctgaggct cgcgaagctg gggggtgcag atctacgtgg
     7861 cgccgacctg
//
LOCUS       DASWDC010000354         3993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1155592, whole genome shotgun sequence.
ACCESSION   DASWDC010000354 DASWDC010000000
VERSION     DASWDC010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155592"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..2936
                     /gene="alaS"
                     /locus_tag="VF995_11350"
     CDS             270..2936
                     /gene="alaS"
                     /locus_tag="VF995_11350"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX9344196.1"
                     /translation="MNANEIRATFTGFFVERGHRLVPSSPLIPNDPTLLLANAGMNQF
                     KPYFLGETVPEYTRATSVQKCTRTSDIENVGDASHCTFFEMLGNFSFGDYFKEGAIAY
                     AWELVTERFGLDPERLWVTVYLDDDEAIELWREVGVPPERIQRLGKDDNFWSMGVPGP
                     CGPCSEIHYDRGPEFGPGDGGPASESTRYLEIWNLVFMQNIRDEDYNIVGDLPKKNID
                     TGMGLERIAMILQDAPTMYETDLLAPVLHEVQEVSGRAYGSNGRTDVSIRIVTEHARS
                     AAFLIADGVLPSNEGRGYILRRLLRRAVRHARLLGVEGGDPALPLVTDRVVDNLGAAW
                     PELVAQRSLISQVVASEEESFARTLRQGSQILEGAIARARDEEATALAGEVAFQLHDT
                     YGFPFELTLEAAEEAGLAIDRDRFEQLMDEQRRRAKEARKELAGAQRKLEAYRDLSAR
                     FGRTTFTGYDESSTAARLLGLLDPDGEELASATEGQQVELILDRTPFYAEAGGQVGDQ
                     GRIHTSDGALLEVTGTRFGLEGFTVHQARVLGGEVHPDQTVLAEVDEARRAAVARSHS
                     ATHVLHWALRRALGDHARQQGSLVDAGRLRFDFAHFSAVAPEELAGIEENVNRHLLGD
                     PEVRVWHTSLADAEAAGAIGLFGEKYGAVVRVVDIGDFSRELCGGTHVGHGSQVGPVR
                     ILGESSIGANLRRIEAFTGMDALRFFDRERKLLEEVAALLRTRPDDAPEQLRRRLEAL
                     ADAQKELERLRLANLEAKAAELAGQARPSGGHADADGAWLVIEQVPDTATDDLRRMAT
                     LVRERHGGGPGVVVLGSSAGGKAAMVAVITKDLADAGRSAAEVLRPAAQVVGGGAGGK
                     GDLATAGGREPGKLAEALAVAEREARNLVGEP"
     gene            2947..3396
                     /gene="ruvX"
                     /locus_tag="VF995_11355"
     CDS             2947..3396
                     /gene="ruvX"
                     /locus_tag="VF995_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX9344197.1"
                     /translation="MSGRVLALDVGSRRLGVAVSDPTGTVATPLTTVPRRKVADDAAA
                     LAKLAAEQQARLIVVGLPVTLAGGEGRAATEVRAYVAELAGLLPGIAFELADERLSTV
                     AAERALIGGGVRRRERRDVVDQVAASLFLQTWLNMRQGERRGREASG"
     gene            3393..>3993
                     /locus_tag="VF995_11360"
     CDS             3393..>3993
                     /locus_tag="VF995_11360"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX9344198.1"
                     /translation="MKLNWEPGTYVGRAERRARKRRRARGIALMLALVLVVVGGTFEL
                     YRLTAAPRRPQVAAGLKVSFTITPGEGTMQIADALAKAGVVSSAGRFRAAADQRGVSG
                     ALKPGTYQLLTGMNVDDLLDLLVRGPATGPRFTIPEGSTVLQIIARIAATRHFSAVQL
                     QHAMASPDLNVPYRPKGVTSMEGLLIPLTYDINKGDSAVD"
BASE COUNT          588 a         1506 c         1398 g          501 t
ORIGIN      
        1 agctcgctcc ccaatcggtg gccaaacgcg cgctggcggc ggccagcgac tggcgggagc
       61 gcctcgcgga cgcctacgac gagggccgcg ccgccatgga tgagcgggag gcggagctgc
      121 gagctctcct cgccgagccg gggaacgaga cgccgtaagt cccaggtcca gcagcaacaa
      181 actggacgtc tcggcctggc tcggcgggat ggaggtttcc acctgcggct agccgccgac
      241 acctgagggt tgatgagccc atggacaaga tgaacgccaa cgagatccgc gcgaccttca
      301 ccggcttctt cgtggagcgg gggcaccggc tggtgccctc cagcccgctg atccccaacg
      361 accccacgct gctgctcgcc aacgccggca tgaaccagtt caagccgtac ttcctcggcg
      421 agaccgtgcc ggagtacacc cgcgcgacca gcgtgcagaa gtgcacccga accagcgaca
      481 tcgagaacgt cggcgacgcc agccactgca ccttcttcga gatgctgggc aacttcagct
      541 ttggcgacta cttcaaggaa ggcgccatcg cctacgcctg ggagctggtc accgaacggt
      601 tcggccttga ccccgagcgg ctgtgggtca ccgtgtacct cgacgacgac gaggcgatcg
      661 agctgtggcg cgaggtcggc gtcccccccg agcggatcca gcggctcggc aaggacgaca
      721 acttctggtc gatgggcgtg cccggcccct gtgggccgtg ctcggagatc cactacgacc
      781 gcgggccgga gttcgggccc ggcgacggtg gcccggccag cgagtccacc cgctatctgg
      841 agatctggaa cctggtgttc atgcagaaca tccgggacga ggactacaac atcgtcggcg
      901 acctgcccaa gaagaacatc gacaccggca tgggcctgga gcggatcgcg atgatcctgc
      961 aggacgcgcc gaccatgtac gagaccgacc tgctcgcccc ggtcctccac gaggtccagg
     1021 aggtctccgg ccgcgcctac ggcagcaacg gccgcaccga cgtctccatc cggatcgtca
     1081 ccgagcatgc ccgctcggcg gcgttcctga tcgccgacgg ggtgctgcca tccaacgagg
     1141 gccgcggcta catcctgcgg cggctgctgc gccgcgcggt gcgccacgcc cgcctgctcg
     1201 gcgtcgaggg cggcgaccca gccctgccgc tggtcaccga ccgggtggtc gacaaccttg
     1261 gcgcggcctg gccggagctg gtcgcgcaac gctccctgat cagccaggtg gtcgccagcg
     1321 aggaggagtc cttcgcccgg acgctccgcc agggctccca gatcctggag ggcgccatcg
     1381 cccgtgcccg cgacgaggag gccaccgccc tcgccgggga ggtcgccttc cagctgcacg
     1441 acacctacgg gttccccttc gagctgacgc tggaggcggc cgaggaggcg ggcctggcga
     1501 tcgaccggga ccggttcgag cagctcatgg acgagcagcg ccgccgcgcc aaggaggccc
     1561 gcaaggagtt ggccggcgcg cagcgcaaac tggaggccta ccgggacctg tcggcgcgct
     1621 tcggccgcac caccttcacc ggctatgacg agtccagcac cgcggcgcgc ctgctcggcc
     1681 tgctcgaccc cgacggcgag gagctggcca gcgctaccga gggccagcag gtcgagctga
     1741 tcctcgaccg cacccccttc tacgccgagg ccggcggcca ggtcggcgac cagggcagga
     1801 tccacaccag cgacggcgcc ctgctggagg tcaccggcac ccgcttcggc ctggaaggct
     1861 tcaccgtcca ccaggcccgc gtcctgggcg gcgaggtgca ccccgaccag accgtcctcg
     1921 ccgaggtcga cgaggcccgc cgggcggcgg tggcgcgcag ccacagcgcc acccacgtgc
     1981 tccactgggc gctgcgccgg gcgctcggcg accacgcccg ccagcagggc tcgctggtcg
     2041 acgccgggcg gctgcgcttc gacttcgcgc acttctccgc ggtcgcgccc gaggagctcg
     2101 ccgggatcga ggagaacgtc aaccggcacc tgctcggcga ccccgaggtg cgggtgtggc
     2161 acaccagcct cgccgacgcc gaggcggccg gcgcgatcgg gctgttcggc gagaagtacg
     2221 gcgcggtggt gcgggtggtc gacatcggcg acttctcccg cgagctgtgc ggcggcaccc
     2281 atgtcggcca cggcagccag gtcggcccgg tccgcatcct cggggaatcc tcgatcggcg
     2341 ccaacctgcg ccgcatcgaa gcgttcaccg gcatggacgc gctgcgcttc ttcgaccggg
     2401 agcgcaagct cctcgaagag gtcgccgcgc tgctgcgcac ccgccccgac gacgcccccg
     2461 agcagctccg ccgccgcctc gaggcgctcg ccgacgcgca gaaggagctg gagcgcctcc
     2521 ggctcgccaa cctggaggcc aaggcggccg agctggccgg ccaggcccgc ccgtccggcg
     2581 gccacgccga cgctgacggc gcctggctgg tgatcgagca ggtccccgac accgccaccg
     2641 acgacctgcg ccgcatggcg accctggtcc gggagcgcca cggtgggggg cccggggtgg
     2701 tggtgctcgg ctccagcgcg ggcggcaagg ccgcgatggt cgcggtgatc accaaggacc
     2761 tggccgacgc cggccgttcc gccgccgagg tgctgcgccc cgccgcccag gtggtcggcg
     2821 gcggcgccgg cggcaagggc gacctcgcca ccgccggcgg ccgcgagccg ggcaagctcg
     2881 ccgaggcgct cgccgtcgcc gagcgggagg cccgcaacct ggtgggggag ccgtgacgcg
     2941 ccccgtatga gcggacgggt gctcgccctg gatgtcggca gccgccggct cggcgtcgcc
     3001 gtctccgatc ccaccggcac ggtggccacg ccgctgacca ccgtgccgcg ccgcaaggtg
     3061 gccgacgacg ccgccgcgct cgccaagctc gccgccgagc agcaggcccg cctgatcgtg
     3121 gtcggcctgc cggtgacgct ggccggcggg gaggggcggg ccgccaccga ggtccgcgcc
     3181 tacgtcgccg aactcgccgg cctgctcccg gggatcgcct tcgagctggc cgacgagcgc
     3241 ctctcgacgg tcgccgccga acgggcgctg atcggcgggg gcgtgcgccg ccgcgagcgg
     3301 cgcgacgtgg tcgatcaggt cgcggctagt ctttttctgc agacatggct caatatgcgg
     3361 caaggcgagc gccgggggag ggaggcgtcc gggtgaagct caactgggaa ccagggacct
     3421 acgtcggccg cgccgagcgg cgcgcccgca agcgacgccg cgcccgcggg atcgcgctca
     3481 tgctggccct ggtgctggtc gttgtcggag gcaccttcga gctctaccgg ctcaccgccg
     3541 cgccacgccg gccacaggtc gccgccgggc tcaaggtcag cttcacgatc acgcccggtg
     3601 aaggcaccat gcagatcgcc gacgcgctgg cgaaggccgg ggtggtctcc tccgccggcc
     3661 gcttccgcgc cgccgccgac cagcgcggcg tctccggcgc cctcaaaccg gggacctacc
     3721 agctgctcac cggaatgaac gtcgacgacc tgctcgacct gctggtccgc ggccccgcca
     3781 ccgggccgcg cttcaccatc cccgaaggct ccaccgtcct ccagatcatc gcccggattg
     3841 ccgccaccag gcacttcagc gccgtccagc tgcagcacgc catggccagc cccgacctca
     3901 acgtccccta ccgacccaag ggcgtgacct cgatggaggg gctgctgatc cccctcacct
     3961 acgacatcaa caagggcgac agcgccgtcg acg
//
LOCUS       DASWDC010000355         9814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1575570, whole genome shotgun sequence.
ACCESSION   DASWDC010000355 DASWDC010000000
VERSION     DASWDC010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575570"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF995_11365"
     CDS             complement(<1..204)
                     /locus_tag="VF995_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344199.1"
                     /translation="MPARNRGDDGDLRGPPGVVWEGLRRFARLPGWLEAGVRPDRVRA
                     ALVAAVPELADGGLLLANCRIKRP"
     gene            complement(286..420)
                     /locus_tag="VF995_11370"
                     /pseudo
     CDS             complement(286..420)
                     /locus_tag="VF995_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872991.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            476..1132
                     /locus_tag="VF995_11375"
     CDS             476..1132
                     /locus_tag="VF995_11375"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX9344200.1"
                     /translation="MTADAQPSLLTRLADLPYAEPAAPIGADAAVLLALSPVPGGADD
                     LGLVLVRRPEHMRNHAGQVGLPGGAVEPTDAGPVAAALREAHEEVGLDPAGVRVVGSL
                     PRAYLNVSGFDVLPVVGSWDGQAPLRPNDSEVAGILRPTLRQLADPANHGTIPLERLV
                     GPADLRGRPGPAPSPVFWVDGVAVWGFTAGLLVRFLEALGLPAPPLPPWWPPPRPGTP
                     "
     gene            1359..2162
                     /locus_tag="VF995_11380"
     CDS             1359..2162
                     /locus_tag="VF995_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9344201.1"
                     /translation="MTDAAATAVPPATVQGDGTPVVLVHAFPLDGRMWAPQVEALAGA
                     YQVIVPDLRGFGAARDQVTEPDGTTMDLLADDLARLLDARGLDQVVLGGLSLGSYLAY
                     AFLRRHPGRLGGLILFSTTAAADTDETRAARMELAKRVEGDGIGVLPEAMLPRLLGPT
                     SLEQRPELVERVRALIVEQDPAGVVTAEHCLAARPDSFGDLGAVAVPTLVISGAEDTV
                     TGPDQGRAVASAIRDARFVLVEGAGHLVNLEQPEQVNEALLNFIAPIWI"
     gene            complement(2333..3643)
                     /locus_tag="VF995_11385"
     CDS             complement(2333..3643)
                     /locus_tag="VF995_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9344202.1"
                     /translation="MKSREEQAELTSRDPHSPREVQNPQPRTHEARAAELPAATRPAP
                     SQPPDHDRSPAQLLAEHHPDSDADRVRSGALFRWGFFAGLGVLAAVAAALAVYSVRDI
                     LLRVLVAVFLAVSLEPAVRGLTRRGVRRGLAVGIIFTAFLALLTGFLMSVIPPLVNQF
                     GQLVRHFPDYVATLQAKSAHFRDLNDRYHLGTRLEGVIEQLPGRLTGGVVGFTGQVFG
                     ALISFLTVVVFTVYFLLDLPRLRRGLVRLFPVERRERYGHIADIMVEKVGDYMIGRLV
                     IAFIGGSVAFVILEFLRIPYPLPLAIFIALLDLIPLLGHPIGSVAAVLAALVTKGFWP
                     TTVLLIVLLVCYQQVENYLIAPRVLRHSVDISPAAVLLAALVGAAMLGVIGALVAIPI
                     AAAFKVVLVRQIDQYEAEAGSRADHLRRPHLPRLRRRPSDDHHD"
     gene            complement(3651..4085)
                     /gene="mce"
                     /locus_tag="VF995_11390"
     CDS             complement(3651..4085)
                     /gene="mce"
                     /locus_tag="VF995_11390"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX9344203.1"
                     /translation="MRLGRLDHVGIAVNDLAAGRAFYEGVLGLEAAHEEVLEEQGVHE
                     LLFPLGDSWVQLVAPLGPDTPVGRFLAKRGEGVHHVGYAVTDIAGALEGLRASGMEVI
                     EPAPRIGSGGTVVAFVHPSSAHGVLVELVEEGTGHRGRPSGA"
     gene            complement(4138..4890)
                     /locus_tag="VF995_11395"
     CDS             complement(4138..4890)
                     /locus_tag="VF995_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344204.1"
                     /translation="MTAATASLGFVHACEQNHLQLCLIGGGAVTQLESGKAVTCPLDP
                     VFVFNYVFGVRRAFDLEGAREAFLAAGRPYVHVMASPSSRADLGEQLGAAGLRVVERQ
                     AFRRATGTGAGAPGLVEAGMGALDELLRLWWAAWGAQRAGKRGEVFRLRLQDGRSRAF
                     RSGDGSGMFLLFDAGPTTQLIHLGVDAAAQGRGTGRRMLELAPGLVPAGRPLWLFTEL
                     GGGGDRTAAAAGWELAHTAEDWMLDLDPGPDD"
     gene            4990..6180
                     /locus_tag="VF995_11400"
     CDS             4990..6180
                     /locus_tag="VF995_11400"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014154025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9344205.1"
                     /translation="MSERNGAVICAGARTPIGRFGGALAGLTAIELGGVAIAAALDRA
                     GVARDTVEHVIMGHVLQAGQGQITARQAAVRAGIPMTVPALTVNKVCLSGTNAIATAA
                     QYVALGQAGTLVAGGMESMSNAPYLLPKGRFGARMGHAELVDAMIWDGLWDAFDDVHM
                     GGLSDRANQRLGIDRAVQDEWSARSHERAAAAAKTVLCEEIAPVRVPQRRGDPILIEE
                     DEGVRPGTTAEALGKLKPAFGADGTITAGNASQISDGAAAVVVTSRDRAEREGLPVLA
                     EVLAYGTVAGPDPSLHSQPSRAIRDACRRGGVAVEDLDLVEINEAFAAVALQSARELG
                     VDEAIVNVNGGAVALGHPIGASGARLVLTCALELRRRGGGLGAAALCGGGGQGDALLL
                     RVEG"
     gene            6217..7203
                     /gene="meaB"
                     /locus_tag="VF995_11405"
     CDS             6217..7203
                     /gene="meaB"
                     /locus_tag="VF995_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX9344206.1"
                     /translation="MPPADTPARELLGRLHAGDKRAVARLISLLEDGARARLREVAET
                     LNPITGRARVLGLTGAPGVGKSTLCDALVAAFRAQGRTVGVLAVDPSSPFTGGALLGD
                     RVRMQRHALDDGVYIRSMATRGQLGGLAWATPQAVRVLDAAGCELVLIETVGVGQAEV
                     EVAGLADTTLVVLAPGFGDAVQVAKAGILEVADLFVVNKADRDGADTVARDLRQMLHL
                     GEERPWDVPVVKTVAERGDRTRDGDSGVGELVERIAAHHAYLEASGALERRRRARAGR
                     EVEEIALSSLRAELGGLGHGEALDSLAEQVAAGKLGPYSAADQLLDQVRGPR"
     gene            7428..8573
                     /locus_tag="VF995_11410"
     CDS             7428..8573
                     /locus_tag="VF995_11410"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX9344207.1"
                     /translation="MVIGVAVALLMSSGGPATAGTLAPAAPAASPAAGDVVQAQPTTI
                     HLDPLGLLPVPVRAWHVLYRSTSALGRPDVVSGTLLVPFLPYGGDRPIIGYAPGTHGM
                     GDQCAPSQKLADGQDVELLVIGQFLLRGWAVAVTDYEGLGTPGDHTYAVGISEGHAVL
                     DVARAASRVGGAGLSPDAPVVLYGYSQGGQAAGWAAQQAASYAPDLPVKGVAAGGVPA
                     DLVRVLHNLEGTSEFGLAFAAGAGFDTAYPELHLEHYLNPRGQAALDRIRGECSDSEL
                     GGLSLSDLTTSDPMTTPAWQARLRENRLGAVAPKMPVLLYHGEADEIIPYGVGTTLKD
                     DWCAKGAALTWVSYAGASHVGAAAEGSLMVTDWIHRLLFGDDIPNSC"
     gene            8739..>9814
                     /locus_tag="VF995_11415"
     CDS             8739..>9814
                     /locus_tag="VF995_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9344208.1"
                     /translation="MREHQAHDATPLEQLEALEQQRDRWRRRYQAAGERDADFTTLSG
                     EPNPPLVSPLDHPERDEAAAIGFPGEYPYTRGIHATMYRGRPWTIRQFSGFGNARETN
                     ARYRYLIAQGGGGLSVAFDMPTLMGRDSDDPRALGEVGHCGVAIDSAADMEILFDGLP
                     LERITTSMTISGPAPMLFAMYLVAAERQGAEIARLDGTLQTDILKEYIAQKEWLFPPE
                     PHLRLIADLLEFCAAEVPKYHPISVSGYHIREAGSTAAQELAFTLADGFAYIELARRR
                     GLDAETVAAQLSFFFDAHIDFFEEIAKFRAARRIWARWLRDRYGLRSEAALRLKFHTQ
                     TAGVSLTAQQPGNNVVRTALEALSA"
BASE COUNT         1377 a         3706 c         3494 g         1237 t
ORIGIN      
        1 cggccgcttg atccggcagt tggccagcag gagcccgccg tccgccagct cgggaacggc
       61 ggcgaccagc gccgcccgga cccggtcggg ccgcaccccc gcctccagcc accccggcag
      121 cctggcaaag cggcgcaacc cctcccagac cacccccggc ggcccccgga ggtcgccgtc
      181 gtcaccgcgg ttgcgggcag gcaccgcgcc tctcctctgc cgatgggaac ccggccgctc
      241 caacagtagc ggccggttgg tttgaggccg atgaggagcg ggccgtcagc cggtcggcag
      301 gcgcagccgg tagccgaccc cgcggaccgt ctccaggtcg cagtggccga acggccggtc
      361 gagcttgcgg cggagatagg ccacgtattg gtcgaccacg ttcgaggtgg ggtcgtaggc
      421 accaccgtgt cccgaggccg gccgatcagg gactccgcgc ctatccttgg tcgcgatgac
      481 cgccgacgca caaccttccc tgctgacgcg tttggccgat ctgccctacg ccgagcccgc
      541 cgcccccatc ggggcggacg ccgccgtgct gctggcgctc tcgccggtcc ccggtggcgc
      601 cgacgacctc gggctggtcc tggtgcgacg gcccgaacac atgcgcaacc acgccggcca
      661 ggtcggcctg cccggcggcg cggtcgagcc caccgacgcc ggcccggtcg ccgcggcgct
      721 gcgcgaagcc cacgaggagg tcggcctcga cccggccggg gtccgggtgg tcggcagcct
      781 cccgcgcgcc tacctgaacg tctccggctt cgacgtcctc cccgtggtcg gctcctggga
      841 cggccaggcg cccctgcggc ccaacgacag cgaggtcgcc ggcatcctcc ggccaaccct
      901 gcgccagctc gccgaccccg ccaaccatgg caccatcccc ctggaacgcc tggtcggccc
      961 cgccgacctg cgcggccgcc ccggcccggc gccaagcccc gtcttctggg tcgacggcgt
     1021 cgccgtctgg ggcttcaccg ccggcctcct ggtccgcttc ctcgaagccc tcggcctccc
     1081 cgccccgccc ctcccaccct ggtggccacc cccacggccg ggtactccat aagcactcgg
     1141 atcgttccgg aagtggcagc ccgcgagttg ccgggatgcc tggacccgcg cggcgcggct
     1201 gctgggcgtg gcagagcccg ctggccctgg tccggtcgac ctgacagatc gacccaggca
     1261 tcgtcccttg aattgctgat gcgtgagaca cccgcgatgg ggtatttctg ggtgtcgctg
     1321 ccccagtgtc agtgaccacc gcttcaaggg agacacacat gactgacgcc gcggcgacgg
     1381 ccgttccccc cgccacggtc cagggcgacg gcacccccgt ggtgctggtg cacgccttcc
     1441 cgctcgacgg gcgcatgtgg gcgccgcagg tcgaggcgct ggccggggcc taccaggtga
     1501 tcgtgccgga cctgcgcggg ttcggcgccg cgcgggacca ggtgaccgag cccgacggca
     1561 ccaccatgga cctgctcgcc gacgacctcg cccgcctgct ggatgcgcgc ggcctcgacc
     1621 aggtggtact cggcggcctg tcgctgggca gctacctcgc ctacgccttc ctccggcgcc
     1681 atcccggccg gctcggcggg ctgatcctgt tcagcaccac cgccgccgcc gacaccgacg
     1741 agacccgcgc cgcacgcatg gagctggcca agcgggtgga gggcgacggg atcggcgtcc
     1801 tccccgaagc gatgctgccc cggctgctcg gcccgaccag cctggagcag cggcccgagc
     1861 tggtcgagcg ggtgcgggcg ctgatcgtgg agcaggaccc cgccggggtg gtcacggccg
     1921 agcactgcct ggcggcacgg ccggactcct tcggcgacct tggcgcggtg gcggtcccga
     1981 cgctggtgat cagcggcgcc gaagacacgg tcaccggccc ggaccaggga cgagcggtgg
     2041 cctcggcgat ccgcgacgcc cgttttgtcc tggttgaggg ggccggtcac ctggtcaatc
     2101 tggagcagcc ggagcaggta aacgaggcgc tgctcaattt catcgcgccg atttggattt
     2161 gattcgcccg caaccggtga caacgcgaca aggcatttag tacgctcatg ggcgccgttg
     2221 gttccgagag cccccaaacg gagccgaaga gcgcgagggg ggcaggggag gaccggccct
     2281 tgcccccctc gcctatgccc tcgcctatgc ccgcatatgc ccgcacgcgg tctcagtcgt
     2341 ggtggtcgtc gctcgggcgg cggcgaaggc ggggcaggtg cgggcggcgg aggtggtccg
     2401 cccttgagcc ggcctccgct tcgtactggt cgatctggcg gaccagcacg accttgaagg
     2461 cggcggcgat cgggatcgcc accagcgcgc cgatcacgcc cagcatggcg gcgccgacca
     2521 gcgcggccag cagcaccgcg gcgggggaga tgtccaccga gtggcgcagc acgcgcgggg
     2581 cgatcaggta gttctccacc tgctggtagc acaccagcag cacgatcagc agcacggtgg
     2641 tgggccagaa ccccttggtg acgagggcgg ccagcaccgc ggcgaccgag ccgatcgggt
     2701 ggccgagcag cgggatcagg tccagcagcg cgatgaagat cgccagcggc agcgggtagg
     2761 ggattcgcag gaactcgagg atcacgaagg cgaccgagcc gccgatgaag gcgatcacca
     2821 gccgcccgat catgtagtcg ccgaccttct cgaccatgat gtcggcgatg tggccgtagc
     2881 gttcacgccg ctcgaccggg aacaggcgca ccaggccgcg gcgcaggcgg ggcaggtcca
     2941 gcaggaagta gacggtgaag accaccaccg tcaggaacga gatcagcgcg ccgaacacct
     3001 ggccggtgaa gccgaccacc ccgccggtga gccgccccgg cagctgctcg atcacgccct
     3061 ccagacgggt gccgaggtgg tagcggtcgt tcaggtcgcg gaaatgggcg gacttggcct
     3121 gcagggtggc gacgtagtcg gggaaatggc gcaccagctg gccgaactgg ttcaccagcg
     3181 gtgggatcac cgacatcagg aacccggtga gcagggcgag gaaggcggtg aagatgatcc
     3241 cgacggcgag cccgcgccgg accccgcggc gggtgagccc ccgcaccgcc ggctccaggc
     3301 tgacggcgag gaacaccgcg accagcaccc gcagcaggat gtcgcgcacc gagtagacgg
     3361 ccagggcggc ggcgaccgcg gccagcacgc cgagaccggc gaagaacccc cagcggaaca
     3421 gcgctcccga ccgcacccgg tccgcgtcgc tgtcggggtg gtgctccgcc agcagctgcg
     3481 ccggggagcg gtcgtggtcg ggtggttgcg agggcgccgg ccgggtcgcg gcggggagtt
     3541 cggcggcgcg cgcctcgtgg gttcggggtt gggggttctg gacctcccgg ggactgtggg
     3601 ggtcccggga ggtcagctcc gcttgctcct ctcgactctt cacggtcgta tcatgctcca
     3661 ctcgggcggc cacggtggcc ggttccctcc tcgaccagct cgaccaggac gccgtgcgcg
     3721 ctgctgggat gcacgaaggc gaccacggtg ccgccggagc cgatccgggg ggccggctcg
     3781 atcacctcca tcccgctggc acgcagcccc tccaaggcgc cggcgatgtc ggtgacggca
     3841 tacccgacgt ggtggacgcc ctcacctcgc ttcgccagaa accgcccaac cggcgtgtcc
     3901 gggccgagcg gcgcgaccag ctgcacccag ctgtcgccga gcgggaacag cagctcgtgg
     3961 acgccttgct cctcgagcac ctcctcgtgc gcggcctcca ggccgagcac gccctcatag
     4021 aaggcgcgcc cagcggccag gtcgttgacc gcgatgccga cgtgatccag gcgtccaagc
     4081 cgcaaagtgg gttccttccc atcgtgtccg cgttcccgtc caagcctcag ccaacgttca
     4141 atcgtccgga ccggggtcca gatcgagcat ccagtcctcg gcggtgtggg ccagctccca
     4201 gcccgctgcc gcggcggtgc ggtccccgcc gccgccgagc tcggtgaaca gccacagcgg
     4261 ccgcccggcc ggaaccaggc ccggcgccag ctccagcatc cgccgcccgg tgccgcgccc
     4321 ctgcgccgcc gcgtccaccc ccaggtggat cagctgggtg gtcggcccgg cgtcgaacag
     4381 caggaacatc ccgctcccgt cgccgctgcg aaaggcgcgg ctgcgcccat cctggaggcg
     4441 gagccggaag acctcccccc gcttcccggc ccgctgggca ccccacgccg cccaccagag
     4501 ccgcagcagc tcgtcgaggg cgcccatgcc ggcctcgacc aggccgggcg cgcccgcccc
     4561 ggtcccggtg gcgcgccgga acgcctgccg ctccaccacc cgcaggccgg cggcgccgag
     4621 ctgctcgccg aggtcggccc gggacgacgg cgaggccatg acatggacgt agggccggcc
     4681 ggcggcgagg aacgcctccc gcgcgccctc cagatcaaag gcgcgccgca ccccgaagac
     4741 gtagttgaag acgaacaccg ggtccagcgg gcaggtcacc gccttccccg attccagctg
     4801 cgtcaccgcg cccccgccga tcaggcagag ctgcaagtgg ttctgctcgc acgcatgcac
     4861 gaacccgagg ctcgccgtcg ccgccgtcat gggcacagcc taaccgagac gagggggacg
     4921 cccccagatg cggcggtaga ctcgaaggcc ggcgttcgcc gagctggatt cgctgagctg
     4981 gagggctgga tgtccgagcg gaacggagcc gtcatctgcg ctggggcgcg cacgccgatc
     5041 ggccgcttcg gcggggcgct ggcggggctg accgccatcg agctgggcgg tgtcgccatc
     5101 gccgccgcgc tagatcgggc gggggtcgcg cgcgacacgg tcgagcacgt gatcatgggg
     5161 cacgtcctgc aggcggggca gggccagatc accgcgcggc aggcggcggt ccgcgccggc
     5221 atccccatga ccgtcccggc gctcaccgtc aacaaggtct gcctgtcggg taccaacgcg
     5281 atcgccaccg ccgcccagta cgtcgccctc ggccaggcgg gcacgctggt cgccggcggg
     5341 atggagtcga tgagcaacgc cccctacctg ctgcccaagg gccgcttcgg ggcgcgcatg
     5401 ggccatgccg agctggtcga cgcgatgatc tgggacggcc tctgggacgc cttcgacgat
     5461 gtccacatgg gcggtctctc cgaccgggcc aaccagcggc tcggcatcga cagggcggtc
     5521 caggacgagt ggtcggcccg ctcccacgag cgcgcggcgg ccgccgccaa gaccgtcctg
     5581 tgcgaggaga tcgccccggt cagggtgccg cagcggcgcg gcgacccgat cctcatcgag
     5641 gaggacgagg gcgtgcgccc cggcaccacc gccgaggcgc tcggcaagct caagccggcc
     5701 ttcggcgccg atggcaccat caccgccggc aacgcctcgc agatctccga cggcgccgcc
     5761 gccgtggtgg tcacctcccg cgaccgggcg gagcgcgagg gcctgccggt gctcgccgag
     5821 gtgctcgcct acggcaccgt cgccgggccc gacccgagcc tgcactcgca gccgtcgcgg
     5881 gccatccgcg acgcctgccg gcgcggcggc gtggccgtgg aggacctcga cctggtggag
     5941 atcaacgagg cgttcgccgc cgtggcgctg cagtcggccc gcgagctcgg cgtggacgag
     6001 gcgatcgtga acgtcaacgg cggcgcggtc gcgctcggcc acccgatcgg ggcgagcggc
     6061 gcccgcctgg tgctcacctg cgcgctggag ctgcgccgcc gtggcggcgg cctcggcgcg
     6121 gccgccctgt gcggcggcgg gggccagggg gacgccctcc tgctccgggt cgaagggtaa
     6181 ccatgacgct caggctgagg gtgacggtga ggccgcgtgc cgccggctga cacccccgcc
     6241 cgggagctgc tgggccggct ccacgcgggc gacaagcgcg cggtggcgcg gctgatctcg
     6301 ctgctggagg acggcgcgcg ggcgcggctc cgcgaggtcg ccgagacgct gaaccccatc
     6361 accgggcggg cgcgggtgct cggcctgacg ggagcgcccg gggtcggcaa gtcgaccctc
     6421 tgcgacgcgc tggtggccgc cttccgcgcc cagggccgca ccgtcggggt gctggcggtc
     6481 gacccgtcgt cgccgttcac cggcggggcg ctgctcggcg accgggtgcg gatgcagcgc
     6541 cacgcgctcg acgacggcgt ctacatccgc tcgatggcga cccgcggcca gctcggcggc
     6601 ctcgcctggg cgacccccca ggccgtccgg gtgctggacg ccgccggctg cgagctggtc
     6661 ctgatcgaga ccgtcggcgt cggccaggcc gaggtcgagg tcgccggcct cgccgacacc
     6721 accctggtgg tgctcgcccc cggcttcggc gacgccgtcc aggtggccaa ggccggcatc
     6781 ctcgaggtcg ccgacctctt cgtggtcaac aaggccgacc gcgacggcgc cgacaccgtc
     6841 gcccgcgacc tgcgccagat gctgcacctc ggcgaggaac ggccatggga cgtgccggtg
     6901 gtcaagacgg tcgccgagcg cggcgaccgc acccgcgacg gcgacagcgg cgtcggcgag
     6961 ctggtcgagc ggatcgccgc ccaccacgcc tacctggagg ccagcggcgc gctggaacgc
     7021 cgccgccgcg cccgcgccgg ccgcgaggtc gaggagatcg ccctcagcag cctccgcgcc
     7081 gaactgggcg gcctcggcca cggcgaggcg ctcgacagcc tcgccgagca ggtcgccgcc
     7141 ggcaagctcg gcccctacag cgccgccgac cagctgctcg accaggtccg cggcccgcgc
     7201 tgagctgacc tcgggccagc ggccgggtag tggatctggc tgatcggatc ggttacgctc
     7261 gcgaccattc cgatccgatt acacacaacg agcagttcag gggcttcgcg cgccacggac
     7321 ctggcggcgg gcggccgaaa aactgccagg aaagggtgag gatgcccccc agctcccgtg
     7381 tcacgcaccg ggccaagcgg tgcctgtacc gagtcctgca gccagccatg gtcatcgggg
     7441 tggcggtcgc gctgctgatg tcttccggag ggccggccac cgccgggacg ctcgcgccgg
     7501 cggcgccggc cgccagccca gcagccgggg acgtcgtgca ggcacagccc accaccatcc
     7561 atctcgatcc gctcgggctc ctgcccgtgc cggtccgcgc gtggcacgtc ctgtaccggt
     7621 cgacgtcggc cctggggagg ccggacgtgg tgtccggcac cctgctggtc ccgttcctgc
     7681 cctacggcgg ggaccggccc atcatcggct acgccccagg cacccacggg atgggcgacc
     7741 agtgcgcccc ctcccagaag ctggccgacg gccaggacgt cgagctgctg gtgatcggcc
     7801 agttcctgct gcgcggctgg gcggtcgccg tcaccgacta tgaggggctc ggcacccccg
     7861 gcgaccacac ctatgcggtg gggatctccg aaggccacgc ggtgctcgac gtggccaggg
     7921 ccgcgtcgcg ggtgggcggg gccgggctgt cgccggacgc gccagtggtg ctctacggct
     7981 actcccaggg cggccaggcg gccggctggg ccgcccagca ggcggcgagc tacgcgccgg
     8041 acctgccggt caagggcgtc gcggcgggcg gggtgccggc cgacctcgtg cgcgtgctgc
     8101 acaatctcga aggcacctcc gagttcggcc tggccttcgc cgcgggcgcg ggcttcgaca
     8161 ccgcctaccc cgagctgcac ctggagcact acctcaaccc ccgggggcag gcggccctcg
     8221 accggatccg cggcgagtgc agcgactccg agctgggcgg cctgagcctg tcggacctca
     8281 ccaccagcga cccgatgacc acgccggcat ggcaggcgcg gctgcgggag aaccgcctgg
     8341 gtgcggtcgc cccgaagatg ccggtcctgc tctaccacgg ggaagccgac gagatcatcc
     8401 cctacggcgt cggcacgacc ctcaaggacg attggtgcgc caagggcgcg gcgctgacct
     8461 gggtcagcta cgcgggtgcc agccacgtcg gggcggcggc agagggcagc ctcatggtga
     8521 ccgactggat ccaccgcctg ctgttcggtg acgacatccc gaacagctgc tgaccaggat
     8581 cagggaggtg agggcaccgc ggccggcatc cacagcgccg gccgcggtgc ccgctccgtc
     8641 gccagcccgg agccacgcca cggcaggcca ctacaatcgc cccaggccgt cgagccggcc
     8701 gtcatccggc cgtgacggga gctgaacgga ggcgaccctt gcgcgagcac caggcccacg
     8761 acgccacccc gctcgaacaa ctcgaagcac tcgaacagca gcgcgaccgc tggcgccgcc
     8821 gctaccaggc ggcgggggag cgggacgcgg acttcaccac cctgtccggc gagccgaacc
     8881 cgccgctggt ctccccgctc gaccatcccg agcgggacga ggccgccgcc atcggcttcc
     8941 ccggcgagta cccctacacc cgcggcatcc acgccaccat gtaccgtggc cggccctgga
     9001 cgatccggca gttctccggc ttcggcaatg cccgcgagac caacgcccgc taccgctacc
     9061 tgatcgccca gggcggcggc ggcctcagcg tcgccttcga catgccgacg ctgatgggcc
     9121 gcgactccga cgacccgcgg gcactcggcg aggtcggcca ctgcggcgtg gcgatcgact
     9181 cggccgcgga catggagatc ctcttcgacg gcctgccgct ggagcggatc accacctcga
     9241 tgaccatctc cggcccggcg ccgatgctgt tcgccatgta cctggtcgcc gccgagcgcc
     9301 agggcgccga gatcgccagg ctcgacggca ccctgcagac cgacatcctc aaggagtaca
     9361 tcgcccagaa ggaatggctg ttcccgccgg agccgcacct gcgcctgatt gccgacctgc
     9421 tggagttctg cgccgccgag gtgccgaagt accaccccat ctcggtgagc ggctaccaca
     9481 tccgcgaggc gggctccacc gccgcgcagg agctggcctt caccctcgcg gacgggttcg
     9541 cctacatcga gctggctcgg cgccgcggcc tggacgccga gaccgtcgcc gcgcagctgt
     9601 cgttcttctt cgacgcccac atcgacttct tcgaagagat cgccaagttc cgcgccgccc
     9661 gccgaatctg ggcgcgctgg ctccgggacc ggtacggcct gcggagcgag gcggcgctgc
     9721 ggctcaagtt ccacacccag accgccgggg tctcgctcac cgcccagcag cccggcaaca
     9781 atgtcgtccg caccgccctg gaggccctgt cagc
//
LOCUS       DASWDC010000356         5407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1576724, whole genome shotgun sequence.
ACCESSION   DASWDC010000356 DASWDC010000000
VERSION     DASWDC010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576724"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VF995_11420"
     CDS             <1..746
                     /locus_tag="VF995_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344209.1"
                     /translation="AAPPQPPAPPRSWAQVHVVQQDPNPSGEYILNLPFPGRNRRRRT
                     STLLDVTTVSIAAVLALVAVALSITVLAGHLLTAWQVYFVLQSAFGILIVQAFGGGLS
                     TLCTTSQSRLKERIRKLSTVTMAAVAWLVCVTGTFFGYAAYSAKPPARSANLRLYPQR
                     WLHSQPRLAFWDTFGMEWKLHLGWMTPFLATAVAFVALRYGRRVMADPQVRKLLTNVF
                     VLAFATAVIASVLGAIVNVVAPNDFMHRS"
     gene            743..1138
                     /locus_tag="VF995_11425"
     CDS             743..1138
                     /locus_tag="VF995_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344210.1"
                     /translation="MSATQPQPQARRGSRPGTAGQDPPEQEPNGGIVAAVIAAGAACA
                     VLGILTVLAAAVPALADVLTFYAPAGPITGKFILATIAYLIIWANLHFRLRGRELSLS
                     KGFVITLLLLMTGLIGTFPPFYQLFGHGS"
     gene            1186..3087
                     /locus_tag="VF995_11430"
     CDS             1186..3087
                     /locus_tag="VF995_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344211.1"
                     /translation="MPRTRSAQRFGALAIVVGGLIGVTGVVHAQNNAPAAQTPTQAQQ
                     QQQAADVTACRLTMPANALSAQGLATPWILSGARGGRCDETIKDQAAFIDATIFDPAT
                     NALSIYRPLIVNRGTTPAIAPLAPTLPANAVVEISVGFNGDQLFLADANHGADIAAGN
                     CVNGLPGSIFGQEIFCNAPAFYAAANGKVKLPAPGTSSADGQPCPTTESWEIVDQDQS
                     DNTTTNYLVSGDQTAQNTAANAARLGGTPVGNGSDNNLLTRFYGPALNCAPPTATDVT
                     DPANPVQSTSSALQGLNARAQNLAQPATIPANDPFVLRGDQPNLTKLNLYRTQVNQPA
                     VASLQDASTATYCNNLWNLGLAKVEQDQAALRAFGRSPDPATSPDLHLFLLSRFKDSW
                     DNLTCEKLTGIPNPVTVETDNNGLVLSAQVTLPGQTPTSTTEAGPTSTVAPTSTVAPT
                     STDAPTSTDAPTSTAAPTSTVAPTSTVAPTSTAAPTTTIAPTSTAAAPTTAALSSTAA
                     PQTTAAPTTAVPTTAAPVTTDAPATTAAPMTTAAPPTTAAPMATQPVVAQQHKPTTRI
                     TTATQTRTVVLVSDPATPTMRQGGTLPFTGAGDTRLILLISALLVLGGSLLVWHGSTP
                     RSARHTPRH"
     gene            complement(3183..4364)
                     /locus_tag="VF995_11435"
     CDS             complement(3183..4364)
                     /locus_tag="VF995_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9344212.1"
                     /translation="MTASTESEAGACQHIEALLAARNLDLEQRLADTEASFRSLLERI
                     PAMVYTAPIEAPSMLSYVSPGVETLFGEKASNCVGDPGIWDRWLYAEDAPWVHEECER
                     TNETGEPFVAEYRVNAKGGEVRWVRDEAVLVTDPAGQPRFWQGILTDVTDARRSLEKE
                     RAAAEHLRALDELKTTFMQAVSHDLRTPLTTVLGSALTLERSFANLPTDDVADLLGRL
                     VTNARKLYRLLEDLLDLDRLTKGSLAPRRSPVDLGELVRRVVEESSIGDERVVKLDLP
                     ALRTSLDGPKVERIVENLLGNLRHTPADTMVWIRLRATPEGALLIVEDDGSGVPPELR
                     DKIFQPFRQADSANAHAPGSGIGLALVARFATLHGGRAWVEDRHGGGASFRVLLPDAP
                     R"
     gene            complement(4482..>5407)
                     /locus_tag="VF995_11440"
     CDS             complement(4482..>5407)
                     /locus_tag="VF995_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9344213.1"
                     /translation="AAWAARVERLRGDAEAGTLPGAAEGLVRVGAAIHSVRAVAPEEL
                     GEVAAWARAAGVPLHVHLSEQVAENDDCLAHTGLRPTALLDEAGALGPGTTAVHATHL
                     DGDDLRRLGAAGAFACFCPTTERDLGDGIGPAAELRDAGVRLCVGSDSNAVVDPFEET
                     RAVELDDRLRLGRRGVHDPDELLAAATANSYAALGWDPVALRPGSLADFVAVSFASRR
                     LAGAVPGAGEAADPDLAAAVLFAAAPDDVTDVVVAGRQVVRDGRHVRCDDVAGLLSAA
                     ARHLQGAGQAHSARAAPASRPDRQEKRNAGT"
BASE COUNT          786 a         2088 c         1830 g          703 t
ORIGIN      
        1 cggccgctcc gccgcagccg ccggccccgc cgcggagttg ggcgcaggtg cacgtcgttc
       61 agcaggaccc gaacccgtcc ggggagtaca tcctcaacct cccgttcccg ggccggaacc
      121 ggcgtcgccg caccagcacg ctgctggacg tgaccacggt ctcgatcgcg gcggtgctgg
      181 cgctggtggc ggtggcgctc tccatcaccg tgctggcggg ccacctgctc accgcctggc
      241 aggtgtactt cgtcctgcag tcggccttcg gcatcctgat cgtccaggcc ttcggcggcg
      301 ggttgagcac cctctgcacc accagccaga gcaggctgaa ggagcgcatc cgcaagctca
      361 gcacggtgac catggcggcg gtggcgtggc tggtctgcgt cacggggacc ttcttcggct
      421 acgccgccta cagcgccaaa ccgccggcac gaagcgcgaa cctgcgcctc tacccgcagc
      481 ggtggctgca ctcccagccg cgcctggcct tctgggacac cttcgggatg gagtggaagc
      541 tgcacctcgg ctggatgacg ccgttcctgg ccacggcggt cgccttcgtc gcgctccgct
      601 acggcaggcg ggtgatggcc gacccgcagg tgcgcaagct gctcaccaac gtcttcgtgc
      661 tcgccttcgc caccgcggtg atcgcctcag tcctcggcgc gatcgtgaac gtggtcgccc
      721 ccaacgactt catgcaccga tcatgagcgc cacccagccc cagccccagg cccgccgggg
      781 ctcccgcccg ggcaccgccg gccaggaccc gccggagcag gagccgaacg gcggcatcgt
      841 cgccgcggtg atcgccgccg gggccgcctg cgccgtgctc ggcatcctga cggtgctggc
      901 ggccgcggtc ccggcactgg ccgacgtgct caccttctac gccccagcgg gcccgatcac
      961 cgggaaattc atcctggcca caatcgccta cctgattatc tgggcgaacc tgcatttccg
     1021 gctccgcggc cgcgagctga gcctctcaaa aggcttcgtg atcaccctgc tgctgttgat
     1081 gaccggcctg atcggcacct tccccccctt ctaccaactg ttcggtcacg gatcgtagcc
     1141 cacgtcaatt gctttctcgt tttcggaaga gaggtggctc cccccatgcc acgtactcgg
     1201 tccgcacaac ggttcggcgc cctcgccatc gtggtcggcg ggctgatcgg cgtcaccggc
     1261 gtcgtccacg cgcaaaacaa cgccccggcc gcccagacgc caacgcaggc gcagcaacag
     1321 cagcaggccg ccgacgtgac cgcctgccgg ctgaccatgc cggcgaacgc gctcagcgcc
     1381 cagggtctgg ccacgccctg gatcctcagc ggcgcccggg gcgggcggtg cgacgagacc
     1441 atcaaggacc aggcggcgtt catcgacgcc acgatcttcg atccggcgac caacgcgctg
     1501 tccatctacc gcccgctgat cgtcaaccgg ggcaccaccc cggcgatcgc gccgctggcc
     1561 ccgaccctgc cggccaacgc ggtggtggag atcagcgtcg gcttcaacgg cgaccagctg
     1621 ttcctcgccg acgcgaatca cggcgccgac atcgcggcgg gcaactgcgt caacggactg
     1681 cccgggtcga tcttcgggca ggagatcttc tgcaacgccc cggcgttcta cgccgccgcg
     1741 aacgggaagg tgaagctccc ggcgccgggc acctcctccg ccgacgggca gccgtgcccg
     1801 accaccgaga gctgggagat cgtcgaccag gaccagtccg acaacaccac caccaactac
     1861 ctggtgagcg gcgaccagac cgcccagaac accgccgcca acgccgcccg gctcgggggc
     1921 acgccggtcg gcaacggcag cgacaacaac ctcctcaccc gcttctacgg tccggcgctg
     1981 aactgcgccc cgccgacggc gaccgacgtc accgacccgg ccaacccggt gcagtcgacc
     2041 tcctcggcgc tgcagggcct caacgcccgc gcgcagaacc tggcgcagcc agccacgatc
     2101 ccggcgaacg acccattcgt gctgcgcggc gaccagccca acctcaccaa gctcaacctc
     2161 taccgcacgc aggtcaacca gcccgcggtc gcgagcctcc aggacgcctc caccgccacc
     2221 tactgcaaca acctctggaa cctcggcctc gcgaaggtcg agcaggacca ggccgccctg
     2281 cgggccttcg gccgctcccc cgacccggcg acctcccccg acctgcacct gttcctgctc
     2341 agccgcttca aggacagctg ggacaacctc acctgcgaga aactcaccgg catccccaac
     2401 cccgtcaccg tcgagaccga caacaacggc ctcgtcctca gcgcccaggt caccctcccc
     2461 ggccagactc cgacatccac caccgaggcc ggccccacct ccaccgtcgc cccgacctcc
     2521 acggtggctc cgaccagcac cgacgccccg acctcgaccg atgcgcccac aagtacggcg
     2581 gctccgacct ccaccgtggc gccgacctcg accgtggctc cgacctccac ggcggctccg
     2641 acgaccacga tcgcccccac ctcgaccgct gccgccccaa ccactgccgc gctaagctcc
     2701 acggccgctc cccagaccac cgcggcaccc acgaccgccg tgccgaccac ggcggccccc
     2761 gtcaccaccg acgctccagc cacgaccgcg gcgcccatga cgactgccgc gccgcctacc
     2821 accgccgcgc cgatggccac ccagcccgtc gtggcgcagc agcacaagcc aacgacgcgg
     2881 atcaccaccg cgacccagac acgcaccgtc gtcctggtca gcgaccccgc cacccccacc
     2941 atgcggcagg gcggcacgct gccgttcacc ggcgcgggcg acaccaggct gatcctgctg
     3001 atctccgccc tcctggtcct cggcggcagc ctcctggtct ggcacggcag cacccccaga
     3061 agcgcacggc acaccccgcg ccactaacca ccccgcaccg gcaccgcgcg atctcgccgc
     3121 cagccccgac caacgacggg gctggcggcg actcacgtca ggcgcgcggg ttcagcgcag
     3181 gttcagcggg gggcgtcggg gagcaggacg cggaaggagg cgccgccgcc gtggcggtct
     3241 tccacccagg cgcggccgcc gtggagggtg gcgaagcggg cgaccagggc gaggccgatg
     3301 ccggagccgg gggcgtgggc gttggcgctg tcggcctggc ggaagggctg gaagatcttg
     3361 tcgcgcagct ccgggggcac gccggagccg tcgtcctcga cgatcaggag ggcgccctcg
     3421 ggggtggcgc ggaggcggat ccagaccatg gtgtcggcgg gggtgtggcg gaggttgccg
     3481 aggaggttct cgacgatccg ctcgaccttg gggccgtcca ggctcgtgcg gagggcgggg
     3541 aggtcgagtt tgacgacgcg ctcgtcgccg atgctggact cctccaccac ccggcggacc
     3601 agctcgccca ggtcgaccgg cgagcggcgc ggcgccaggg aacccttggt gaggcggtcc
     3661 aggtcgagca ggtcctccag cagccggtag agcttgcggg cgttggtgac caggcggccg
     3721 agcaggtcgg cgacgtcgtc ggtgggcagg ttggcgaagc tccgctccag cgtgagggcg
     3781 ctgccgagca cggtggtgag gggggtgcgc aggtcgtgcg agacggcctg catgaaggtg
     3841 gtcttcagct cgtcgagggc gcgcaggtgc tcggcggcgg cccgctcctt ctccagggag
     3901 cggcgggcgt cggtgacgtc ggtgaggatc ccctgccaga agcgcggctg cccggccggg
     3961 tcggtgacca ggaccgcctc gtcacggacc cagcggacct cgccgccctt ggcgttgacg
     4021 cgatactcgg cgacgaacgg ctcgccggtc tcgttggtcc gctcgcactc ctcgtggacc
     4081 cagggggcgt cctcggcgta cagccagcgg tcccagatcc ctgggtcgcc gacgcagttg
     4141 ctggccttct cgccgaacag cgtctcgaca ccggggctga cgtagctgag catgctcggc
     4201 gcctcgatcg gggcggtgta gaccatcgcc gggatgcgtt cgagcagcga ccggaagctc
     4261 gcctccgtat cggccaggcg ctgctccagg tcgagattac gcgccgcgag cagtgcctcg
     4321 atgtgctggc aggcgccagc ctccgactca gtcgacgctg tcacggccct tcacctcgcc
     4381 cgagtacccg ttccggtcca agtaccggtc caagcagttt cccacggacc atgctgtcta
     4441 accaattcgg ccgagacgcg ctacaagcgc cagggggccg gtcaagtacc ggcgttgcgc
     4501 ttttcctgcc tgtctgggcg ggaggcgggg gcagcgcgag ccgagtgtgc ttgaccggcc
     4561 ccctggaggt gacgggcggc ggcgctgagc agtccggcga cgtcgtcgca gcggacatgg
     4621 cggccgtccc tgaccacctg gcggccggcg accaccacgt cggtgacgtc gtcgggcgcg
     4681 gcggcgaaca gcacggcggc tgccaggtcc ggatcggcgg cctcgcccgc cccagggacc
     4741 gcgccggcga ggcgccggga ggcgaagctg acggcgacga agtcggccag gctgccagga
     4801 cgcagggcca ccgggtccca gccgagggcg gcgtagctgt tggcggtggc ggcggcgagc
     4861 agctcgtcgg gatcgtggac gccgcggcgc cccaggcgca ggcggtcgtc cagctcgacg
     4921 gcgcgggtct cctcgaacgg gtccacgacc gcgttggagt cgctgccgac gcagagccgc
     4981 accccggcgt cgcgcagctc ggcggctggg ccgatgccgt cgcccaggtc gcgctcggtg
     5041 gtcgggcaga agcaggcgaa cgcgcccgcc gcgccgaggc ggcgcaggtc gtcgccgtcg
     5101 aggtgggtgg cgtgcacggc ggtggtcccc gggccgaggg cgccggcctc gtccagcagc
     5161 gcggtggggc gcagcccggt gtgggcgagg cagtcgtcgt tctcggcgac ctgctcggag
     5221 aggtggacgt gcagcggcac ccccgccgcg cgcgcccagg cggcgacctc gcccagttcc
     5281 tcgggcgcga ccgcgcggac gctgtggatc gccgcgccga cccggaccag cccctccgcg
     5341 gcgcccggca gcgtcccggc ctcggcgtcg ccgcgcaggc gctcgacccg ggccgcccag
     5401 gcggcgg
//
LOCUS       DASWDC010000357         9791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1159411, whole genome shotgun sequence.
ACCESSION   DASWDC010000357 DASWDC010000000
VERSION     DASWDC010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159411"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="VF995_11445"
     CDS             complement(<1..1426)
                     /locus_tag="VF995_11445"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX9344214.1"
                     /translation="MQSPPDREQTDGDGAFAKLRRRLPRPTARLVRLPPARQLWPPRL
                     LRQPHPLLAAVIALVMVLALFGLDGALAAGRVRHGVTVGDVDLSGLTPSQARSRLLAA
                     ATALQSRELTIRAGSASMRLTVADAGIGLDVNASLTAALAVGRHGPVDPQRLRGWLGG
                     VRLPWRVLTDEPKLARLLATLDRRLDRPSREPSLRVDTTIPTTPSVDLVAGRPGEAID
                     REGAKGALRVLEAAAAAQPQPAAEIRLPVTERAPTVPDAAAQAALQRARALLAGPLTV
                     TTPSGPGAVGTVGEATLQPGDLAPLLRASPAGGQLRLALDPAGLDQLLRRRLPSAYSA
                     ATAATFTTAGSSAHVVPAVPGRALDPAKAAAALLAAGTRPGGDRQVTLPVAPQQPRLT
                     TAAAQALGVKEVLSTFTTTFSAADAPRVHNIGLIAAAVNGSLVMPGQVFSMNGATGER
                     TAAKGYRTAHVIVNGELVDGLGGGV"
     gene            complement(1578..4097)
                     /locus_tag="VF995_11450"
     CDS             complement(1578..4097)
                     /locus_tag="VF995_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9344215.1"
                     /translation="MKAVIMAGGEGTRLRPLTANQPKPMLPLANRPIMEHIVHHVRNS
                     GIKDIVVTVQFLASQVRNYFGDGSDMGVDLTYATESTPLGTAGSVRNAAEQLDETFVV
                     ISGDALTDISLDEVVAFHRKRGAMVTVALKQMENPLEFGIVITQEDGRIERFLEKPHW
                     GQVFSDTINTGIYVFEPEIFDHIPDGPADFAADVFPRLLAAGLPLFGWVGDGYWEDVG
                     NLEAYQRAHHDILDGKVRVDLRGFEVRPGVWIGEGAEVDPDARLDGPVLIGDFAKVEA
                     SAHLREYTVIGSGVVVKGGAFLHRAIVHDNAYVGPGASLRGCVVGRGADVKRGGRVEE
                     GAVVGDNANIGNGAILQPNVKVYPFKTVEAGATVTKSIIWEGRGARTLFGEHGVTGLA
                     NIDVTPELVLRLSMAFGSTLKKGSTVTLGRDASRVSRTLKRALVAGLNSTGVSCDDME
                     LNPAPVVRFAATRAGASGGIYVRTAPRDSQSIELAVFADDGSDLDEGARRRVERTYNR
                     GEFRRAFGREMGELRYPPRQIHRYIAALLDCLDIPALRARGFKVVLDTSGGTCNLVLP
                     HLLPRLGLVDTMTVNSQLDEGRVAPSTEQERADLDRLARMVTASGADLGVRLDPVGER
                     LILVDERGGIVGPEQALLLYVDLMARSGEAGDFALPVSTTSRAAALAARHGRAVVGTK
                     LAASAITRAAQRDGVVFAGSDSGGYVFPALHPAYDALLAFGKLLELLALQETGLDCAV
                     ARLPGAHVVRREIPTPWERKGAVMRQVVEAAKGRVSDDTDGFKVFHPDPAGEGLDPWA
                     LVVPDTVEPVTHLWAEAATTAAAEALAGEYEALVQAAVAGT"
     gene            complement(4251..4877)
                     /locus_tag="VF995_11455"
     CDS             complement(4251..4877)
                     /locus_tag="VF995_11455"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012182669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9344216.1"
                     /translation="MTHPCEPLSPSPSASASQGPSSAIRTAPNAISFGRLLLMPVCAW
                     LLGSGHYATGIIVTGAVGATDWVDGWLARRTGQVSRLGQLLDPLADRLLIASVAIALL
                     ARGVLPWAAVVLLVGRDLLLLAGWPLLRRRGVRPPEVVWVGKAATFDLLVALPMLVLG
                     ETGLGVAGAAHWLGLVLLWFGVALYYVAGGVYVKMAYDGLAGRVRPGP"
     gene            complement(4918..4994)
                     /locus_tag="VF995_11460"
     tRNA            complement(4918..4994)
                     /locus_tag="VF995_11460"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4958..4960),aa:Pro,seq:ggg)
     gene            complement(5046..6419)
                     /gene="der"
                     /locus_tag="VF995_11465"
     CDS             complement(5046..6419)
                     /gene="der"
                     /locus_tag="VF995_11465"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX9344217.1"
                     /translation="MTPDKAGAAPDPAPAPTAPLPRVAVVGRPNVGKSTLVNRILGRR
                     AAITEELPGVTRDRVTYPAEWSGRRFELVDTGGWEPRASGLQGRVVAQAERAMAEADL
                     LLFVVDATTGITEEDATVAARLRRGPVPVLLVANKVDSAAVEPLATGFWSLGVGEPFS
                     VSSLHGRGAGDLLDEVIARLGEPAPRGAEDAGPPAIAIVGRPNVGKSSLLNRMAGTEV
                     AIVDPTPGTTRDTVDSTVTHDSRRYRLVDTAGMRRAFQHAEGADYYALVRSIEAVERA
                     DLALLVLDAPDGVTEQDQKVAVRAAEAGAGVVLIANKWDLMDDDARTRFDLDLDRKLY
                     FVSWAPLLRTSALTGRNVGKIWQTIDLVLAERARRIPTAALNRWLERATTRTVPPPSR
                     GRSVKVRYATQARANPPEFVLFTTGVLAPAYRRYLENDLRRTFSFQGTPLRLVTRIKS
                     AARDSGR"
     gene            complement(6450..7142)
                     /locus_tag="VF995_11470"
     CDS             complement(6450..7142)
                     /locus_tag="VF995_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9344218.1"
                     /translation="MSTTGSYRVYQAAALVVKPLMRGWFRMRVEGAACVPASGPVILA
                     CNHRSNVDPVLLASAVRRPVTFMAKAELFVGPLRWILNAIEQFPVRRGGIDREALRDC
                     ATVLGRGGVLGLFPEGTRGDGSFTSIHPGIAYVVVRQHCPIVPVAIIGTERVRRRFGS
                     LPWASPVRIVIGAPFELPGVGATADAADAAGVAGADTGGARAARRAATEQLGRVLREF
                     LQQVDTDILTNQ"
     gene            complement(7159..7917)
                     /gene="cmk"
                     /locus_tag="VF995_11475"
     CDS             complement(7159..7917)
                     /gene="cmk"
                     /locus_tag="VF995_11475"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX9344219.1"
                     /translation="MDHDVVVAIDGPAGSGKSTVARAVADRTGLRYLDTGSTYRALTL
                     GLLREGVPVDDPLAVAAGTPEIRLALQLDQAPGAASRVLLDGEELRPEQLRSQAVNDA
                     VSAVSAVPEVRERLVELQRAAMADGGVVAEGRDVGATVWPGAEVKVFLTASAAERARR
                     RAAPTNGGSGSNGPGNGGGESAEAVARRDRLDSRRAASPTRPGEGAVVVDTTDVPVDR
                     VVGQVVELVEAARARARAQTQTQTRAGTQTGHPR"
     gene            complement(8111..9229)
                     /locus_tag="VF995_11480"
     CDS             complement(8111..9229)
                     /locus_tag="VF995_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX9344220.1"
                     /translation="MTATRWPRRLAIIGTGLLGASVGMAARAAGVPEVIGVDADRREL
                     RDAFAREAITERARSVEDAADGADLVVAATPVRALAGVLARAHAVNPTAVLTDVGSTK
                     SHLLVELEGFSTDLGRVIPGHPMAGSEQRGAQAARADLFHGAAWVLTPAAHVDHQALR
                     RVTGFVRGLGGRPLILDPELHDRVVAYASHLPQLAASALMGAVAEVEAPAALRSLVAS
                     GFRDTTRIAASDPDLWVDICATNGPSIVAALDSLSERLTALRGLVASGDRDGLREALT
                     EARSARLRLPAKSGISPRALRHLVVHIPDRPGSLAAVFRVLGAAGVNVEDLSSLDHEL
                     QGGSGDLHIWVAGRDALATAKEALTSAGWIAAEAADNP"
     gene            complement(9403..>9791)
                     /locus_tag="VF995_11485"
     CDS             complement(9403..>9791)
                     /locus_tag="VF995_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX9344221.1"
                     /translation="LLLTNDGPLAYRLTHARFGVEKRYLAEVERLPANAISRLLAGVT
                     LEDGPAKAERARAVASSGRRRMVEIVMMEGRNREVRRLLDAVGAPVRRLVRTAVGPVR
                     LTGLAPGEYRPLRQEEVRRLYQAVGL"
BASE COUNT         1246 a         3455 c         3783 g         1307 t
ORIGIN      
        1 agaccccgcc gccgagcccg tcgaccagct cgccgttgac gatcacgtgg gcggtgcggt
       61 agcccttggc ggcggtacgc tccccggtcg cgccgttcat cgagaagacc tgtccgggca
      121 tgaccaggct gccgttcacc gcggcggcga tcagcccgat gttgtggacc ctgggcgcgt
      181 cggcggcgct gaaggtggtc gtgaaggtcg agaggacctc cttgacgccg agcgcctgcg
      241 cggccgcggt ggtcagccgc ggctgctggg gagcaaccgg cagggtcacc tggcggtcgc
      301 cgccggggcg ggttccggcg gcgagcagcg ccgcggcggc cttggcgggg tcgagggcgc
      361 ggccggggac cgccgggacg acgtgggcgg aggacccggc ggtcgtgaag gtcgcggcgg
      421 tggcggcgct gtaggcggac gggagcctgc ggcgcagcag ctggtcgagc ccggcggggt
      481 cgagggcgag ccgcagctgc ccgccggcgg ggctggcgcg cagcagcggg gcgaggtcgc
      541 cgggctgcag ggtcgcctcc cccacggtcc ccaccgcccc cggcccgctt ggcgtggtga
      601 cggtgagcgg cccggcgagg agggcgcggg cccgctgaag cgccgcctgg gcggcggcat
      661 cggggacggt cggggcgcgt tcggtgacgg ggagacggat ctcggccgcg ggctgcggct
      721 gcgctgcggc ggccgcctcg agcacccgca gcgcgccctt ggcgccctcg cggtcgatcg
      781 cctcgccggg gcggccggcg accaggtcga ccgacggggt ggtggggatg gtggtgtcga
      841 cccgcaggga cggttcccgg ctggggcggt cgagccgccg gtcgagggtg gcgagcaggc
      901 gagcgagctt cggctcgtcg gtcagcaccc gccaggggag ccggaccccg cccagccagc
      961 cgcgcaggcg ctgcgggtcg accgggccat ggcggccgac cgccagagcg gcggtgaggc
     1021 tcgcgttgac gtcgaggccg atgccggcgt cggccacggt cagccgcatg gaggcgctgc
     1081 cggcgcggat ggtcagctcc cggctctgca gcgcggtcgc ggcggcgagc agccgcgacc
     1141 gcgcctgtga cggggtcagc ccggacaggt cgacgtcgcc gaccgtgacg ccgtggcgga
     1201 cccgcccggc ggccagcgcg ccgtcgagcc cgaacagcgc caggaccatg accagggcga
     1261 tcaccgcggc gagcagcggg tggggctggc gcagcaggcg cgggggccac agctggcggg
     1321 ctggcggcag gcggaccagc cgtgccgttg ggcgtgggag gcgccgccgc agcttggcga
     1381 acgccccgtc tccgtcggtc tgctccctgt ctggcggtga ctgcacgacc gcgatggctg
     1441 gctccttacg ctgctccggg ccgcccccac ggcagagctc ccggaccccc tgacgattcc
     1501 acccggggcg catggcagcg gccacccggc gaccggtgaa gcctacgggg aggccgctgc
     1561 gctggcaaac cgcagggtca ggtgccagcg acggcggcct ggaccagcgc ctcgtactcg
     1621 ccggcgagcg cctcggcggc ggcggtggtg gccgcctcgg cccacaggtg ggtgactggc
     1681 tcgacggtgt cggggaccac cagcgcccac gggtcgagac cctccccggc cggatctggg
     1741 tggaacacct tgaaaccgtc ggtgtcgtcg ctgacgcggc ccttggccgc ctcgacgacc
     1801 tggcgcatca ccgcgccctt gcgctcccag ggggtgggga tctcgcggcg gaccacatgg
     1861 gcgccgggga gccgggcgac cgcgcagtcc aggccggtct cctggagcgc aagcagctcc
     1921 agcagcttgc cgaaggccag cagcgcgtcg taggcgggat gcagcgccgg gaacacgtag
     1981 ccgccgctgt cagaaccggc gaacaccacc ccgtcgcgct gcgccgcgcg ggtgatcgcg
     2041 ctggccgcca gcttggtgcc gaccaccgcg cggccgtggc gggcggccag cgcggccgca
     2101 cggctggtgg tcgagaccgg cagggcgaag tcgcccgcct ccccggaacg ggccatcagg
     2161 tcgacgtaga gcagcagggc ctgctccggg ccgacgatgc cgccgcgctc gtcgaccagg
     2221 atgagccgct cgccgaccgg gtcgaggcgc acgccgaggt cggcgccgga tgcggtgacc
     2281 atcctcgcga gccggtcgag gtcggcgcgt tcctgctcgg tcgacggcgc caccctgccc
     2341 tcgtcgagct ggctgttgac ggtcatggtg tcgaccaggc cgagccgtgg cagcaggtgg
     2401 ggcaggacca ggttgcaggt gccgcctgag gtgtccagca ccaccttgaa gccccgcgcc
     2461 cgcagcgctg ggatgtcgag gcagtcgagc agcgcggcga tgtagcggtg gatctggcgg
     2521 ggcgggtagc gcagctcgcc catctcgcgg ccgaaggcgc ggcggaactc gccgcggttg
     2581 taggtccgct cgacccggcg ccgggcgcct tcgtcgaggt cgctgccgtc gtcggcgaac
     2641 accgccagct cgatcgactg gctgtcgcgc ggcgcggtgc gcacgtagat gcccccggac
     2701 gcgcccgcgc gggtggcggc gaaacgcacc accggggccg ggttcagctc catgtcgtcg
     2761 cagctgacgc cggtcgagtt gagcccggcg accagcgccc gcttgagcgt gcgcgagacc
     2821 cggctggcgt cgcggccgag ggtcaccgtc gaccccttct tgagggttga gccgaacgcc
     2881 atcgacaggc gcagcaccag ctcaggggtg acgtcgatgt tggccaggcc ggtgacgccg
     2941 tgctcgccga acagggtgcg ggcgccgcgg ccctcccaga tgatcgactt ggtcacggtg
     3001 gcgccggcct cgaccgtctt gaacgggtag accttcacgt tgggctgcag gatggcgccg
     3061 ttgccgatgt tggcgttgtc gccgaccacc gcgccctcct cgacccggcc gccgcgcttg
     3121 acgtcggcgc cgcggccgac gacgcagccg cgcaggctcg cgccgggacc gacgtaggcg
     3181 ttgtcgtgga cgatcgcgcg gtgcaggaag gcgccgccct tcaccaccac tcccgagccg
     3241 atcacggtgt actcgcgcag gtgcgcgctg gcctccacct tggcgaagtc gccgatcagc
     3301 acggggccgt ccaggcgggc gtcggggtcg acctcggcgc cctcgccgat ccacacgccg
     3361 gggcggacct cgaagccgcg caggtcgacg cggaccttgc cgtcgaggat gtcgtggtgg
     3421 gcgcgctggt aggcctccag gttgccgacg tcctcccagt agccgtcgcc gacccagccg
     3481 aacaggggca ggccggcggc cagcagccgc gggaagacgt cggcggcgaa gtctgcgggg
     3541 ccgtcgggga tgtggtcgaa gatctccggc tcgaacacgt agatgccggt gttgatggtg
     3601 tcggagaaca cctggcccca gtggggcttt tcgaggaagc gctcgatgcg gccgtcctcc
     3661 tgagtgatga cgatgccgaa ctcgagcggg ttctccatct gcttgagggc gacggtgacc
     3721 atggcgccgc gcttgcggtg aaaggccacc acctcgtcga ggctgatgtc ggtgagcgcg
     3781 tcgccggaga tcaccacgaa ggtctcgtcg agctgctcgg cggcgttacg gacgctgccg
     3841 gcggtgccga gcggggtcga ctcggtagcg taggtcaggt cgacgcccat gtcggagccg
     3901 tcgccgaagt agttgcgcac ctggctggcg aggaactgca cggtgacgac gatgtccttg
     3961 atgccgctgt tgcgcacgtg gtggacgatg tgctccatga tcggccggtt ggccagcggc
     4021 agcatcggct ttggctggtt ggccgtgaga ggccgcaggc gggtcccttc cccgcccgcc
     4081 atgatcactg ccttcatcgt cgtggcctcc tcccggtcga tgacgccccc ggggcccgcc
     4141 ggcgggcgcg ggggcaccgc gaccgcttgg cgtaccttcc ctgggagcgt cgcaacaacc
     4201 gtactgcaac aaccgtaccg gccggtgggc accggctcat ggcgagcggg ctaggggccg
     4261 gggcgcaccc gcccggcaag cccgtcatac gccattttga cgtagacgcc gccggccacg
     4321 tagtacagcg ccaccccgaa ccacagcagg accagcccga gccagtgcgc cgctccggcg
     4381 acccccagcc cggtctcccc caacaccagc atcggcagcg cgaccagcag gtcgaaggtt
     4441 gcggccttgc cgacccaaac gacctctggg ggacgaaccc cccggcgccg cagcagcggc
     4501 cagcccgcca gcagcagcag gtccctgccg accagcagca ccaccgccgc ccacggcagc
     4561 accccgcggg ccagcagcgc gatcgccacc gacgcgatca gcaaccggtc agcgagcggg
     4621 tcgagcagct gcccgagccg gctcacctgc ccggtccggc gtgccagcca gccgtccacc
     4681 cagtcggtcg cccccaccgc cccggtgacg atgatcccgg tggcgtagtg cccgctgccg
     4741 agcagccagg cacacaccgg catcagcagc agccgcccga agctgatcgc gttcggggcg
     4801 gtacggatgg cgctcgaggg cccctggctc gccgaagccg acggcgacgg ggacagcggc
     4861 tcgcacgggt gcgtcatgct tgcagcgtag ccgagccgcc ccgtgggcgc ctcgatgtgg
     4921 tcgggacggc gggcttcgat cccgcgaccc ctggtccccc agaccagtgc gctgccaggc
     4981 tgcgccacgt cccgaaacac aagcggggac accgctcgcg ggccacaggc taccacgtca
     5041 ccagctcacc gcccgctgtc cctggcggcg ctcttgattc gggtgaccag gcgcaggggc
     5101 gtgccctgga agctgaaggt gcggcggagg tcgttttcga ggtagcggcg gtaggcgggg
     5161 gcgagcacgc cggtggtgaa caggacgaat tcgggggggt tggcgcgtgc ctgggtggcg
     5221 tagcggacct tgacgctgcg gccccgcgac ggcggcggca cggtgcgggt ggtggcccgc
     5281 tccagccagc ggttgagggc ggcggtgggg atgcggcgag cccgctcggc gagcaccagg
     5341 tcgatggtct gccagatctt gccgacgttt cggccggtca gcgcggaggt gcgcagcagc
     5401 ggcgcccagc tgacgaagta caacttccgg tcgaggtcga ggtcgaaacg ggtgcgggcg
     5461 tcgtcgtcca tcaggtccca cttgttggcg atcagcacca ccccggcgcc ggcctcggcg
     5521 gcgcggacgg cgaccttctg gtcctgctcg gtgacgccgt cgggggcgtc gagcaccagc
     5581 agggcgaggt cagcgcgctc gaccgcctcg atgctgcgga ccagggcgta gtagtcggcg
     5641 ccttcggcgt gctggaaggc gcggcgcatg ccggcggtgt cgaccaggcg gtagcggcgg
     5701 ctgtcgtggg tgacggtgga gtcgacagtg tcgcgggtgg tgccgggggt cgggtcgacg
     5761 atcgcgacct cggtgccggc catgcggttg agcaggctgg acttgccgac gttggggcgg
     5821 ccgacgatgg cgatcgccgg ggggcccgcg tcctcggcgc cgcggggggc cggctcgccg
     5881 aggcgagcga tgacctcgtc gagcaggtcg ccggcgccgc ggccgtgcag gctcgacacc
     5941 gagaacggct cgccgacgcc gagggaccag aagccggtcg ccagcggctc gacggcggcg
     6001 ctgtcgacct tgttggcgac cagcaggacc ggcaccggcc cgcggcgcag gcgcgcggcg
     6061 acggtggcgt cctcctcggt gatgccggtg gtggcgtcga ccacgaacag cagcaggtcg
     6121 gcctctgcca tggcccgttc ggcctgggcg accacgcggc cctgcaggcc gctggcacgg
     6181 ggttcccagc cgccggtgtc gaccagctcg aagcgccggc cgctccactc cgccgggtag
     6241 gtgacccggt cgcgggtgac gccgggcaac tcctcggtga tcgcggcgcg ccgcccgagg
     6301 atgcggttga ccagcgtcga cttgccgacg ttgggccgcc cgaccacggc gacccgcggc
     6361 aacggcgccg tcggcgctgg ggccgggtct ggggccgctc ctgccttgtc tggggtcatg
     6421 tcgtcatgtc gttttagcgg tgagttgact cattgatttg tcaatatatc ggtgtcgacc
     6481 tgctgcagga attcgcgcag gacgcggccg agctgctcgg tggcggcccg ccgggcggca
     6541 cgggcccccc cagtgtcggc gccagcgacg ccagcggcgt cagcggcgtc agcggtggcg
     6601 ccgacgcctg gcagctcgaa cggcgcgccg atcacgatgc gcactgggga agcccacggc
     6661 agcgagccga agcggcgccg gacccgctcg gtgccgatga tcgccaccgg cacgatcggg
     6721 cagtgctgcc gcaccaccac gtaggcgatg ccggggtgga tgctggtgaa gctgccgtcg
     6781 ccacgggtgc cctcggggaa caggccgagc acgccgccac ggccgaggac cgtggcgcag
     6841 tcgcggagcg cctcccggtc gatgccgccg cgccgcaccg ggaactgctc gatggcgttg
     6901 aggatccagc gcagcgggcc gacgaacagc tccgccttgg ccatgaaggt gaccggacgg
     6961 cgtaccgccg aggcgagcag gaccgggtcc acgttgctgc gatggttgca ggcgaggatc
     7021 accgggccgc tggcggggac gcacgcggcg ccctcgaccc gcatgcggaa ccagccgcgc
     7081 atcagcggct tcaccaccag agcggcggcc tggtagacgc ggtaggagcc tgttgtgctc
     7141 acttgtgcac cggcatcgct agcgtgggtg gcccgtctgg gtccccgccc tggtctgggt
     7201 ctgggtctgg gcccgcgccc tggctctggc ggcctcgacc agctcgacga cctggccgac
     7261 cactcggtcg acggggacgt cggtggtgtc gacgaccacc gcgccctcgc cgggtcgggt
     7321 gggtgaggcg gcgcggcgcg agtccaggcg gtcgcggcgg gcgaccgcct cggcggactc
     7381 gccgccgccg ttgcccgggc cgttgctgcc gcttccgccg ttggtggggg cggcgcggcg
     7441 gcgggcccgc tcggcggccg aggcggtgag gaagaccttg acctcggcgc ccggccagac
     7501 ggtggcgccg acgtcacggc cttcggcgac gacgccgccg tcggccatgg ccgcgcgctg
     7561 cagctcgacg agccgctcac gcacctcggg gacggccgag accgccgaga cggcgtcgtt
     7621 gaccgcctgg gagcgcagct gctccggtcg cagctcctcc ccgtccagca gcaccctcga
     7681 ggcggctccg ggcgcctggt caagctggag ggcgagccgg atctccggcg tgccggcggc
     7741 gaccgccagc gggtcgtcga cggggacgcc ctcccgcagc aggccgaggg tgagcgcccg
     7801 gtaggtcgag ccggtgtcga ggtaccggag gccggtccgg tcggcgaccg cacgggcgac
     7861 cgtgctcttg cccgacccgg ccgggccgtc gatcgccacc acgacgtcgt ggtccatgga
     7921 tttacgcctg ccttccacct ggtcgtgctg gtcttctgag ctgggcggca tgagtctact
     7981 catctcatgg cctcatctca tggcccttgg cggctcggcg cgccggctcg gcccgtcgac
     8041 ttgacgatct gttgacttgg cgatctgtcg atccatcgac aaaacaatct gtcgatccgt
     8101 tgacctgtgg tcacgggttg tcggccgcct cagcggcgat ccagcccgcg ctggtgaggg
     8161 cctccttggc ggtggcgagg gcgtcgcggc cggcgaccca gatgtgcagg tcgccgctgc
     8221 cgccctggag ctcgtggtcc aggctgctga ggtcctcgac gttgacgccg gccgcgccga
     8281 gcacgcggaa gaccgcggcc aggctgccgg gccggtcggg gatgtggacc accaggtgcc
     8341 gcagggcgcg gggcgagata cccgacttgg ccggtaggcg cagccgggcg ctgcgcgcct
     8401 cggtgagcgc ctcgcgcagg ccgtcgcggt cgccgctggc gaccagcccc cgcagggcgg
     8461 tgaggcgctc gctgagcgag tcgagcgcgg cgacgatgct ggggccgttg gtggcgcaga
     8521 tgtcgaccca caggtccggg tcgctggcgg cgatgcgggt ggtgtcgcgg aagccgctgg
     8581 cgaccaggct ccgcagcgcg gcgggtgcct ccacctcggc gaccgcgccc atcagcgcgg
     8641 aggcggcgag ctgcggcagg tggctggcgt aggcgaccac ccggtcgtgc agttccgggt
     8701 ccaggatcag cggccgcccg ccgaggccgc gcacgaaccc agtcacgcgc cgcagcgcct
     8761 ggtggtcgac gtgggcggcc ggggtgagca cccaggcggc gccatggaac aggtcggcgc
     8821 gggcggcctg ggcgccgcgc tgctcggacc cggccatggg atggccgggg atgacccggc
     8881 ccaggtctgt ggaaaaacct tcgagttcca cgagaaggtg ggatttggtg ctgccgacgt
     8941 cggtcagcac cgcggtgggg ttgaccgcgt gggcgcgggc gagcaccccg gccagcgctc
     9001 gcaccggcgt ggcggcgacc accaggtcgg cgccgtcggc ggcatcctcg accgagcgtg
     9061 cccgttcggt gatcgcctcg cgggcgaacg cgtcgcgcag ctcccgccgg tcggcgtcca
     9121 ccccgatcac ctcggggacc ccggcggcgc gggcggccat gccaaccgac gcgcccagca
     9181 gcccggtgcc gatgatcgcc agccggcgtg gccatctggt cgccgtcacc ggaccggctc
     9241 ctgctctgcg ttcatgtcac tgcctcgttt cgttgcctgg gagacaaaga gatttgtcga
     9301 cttgtggcgt tgtcgccgcc gcggtcccgc tccagggtcc cgctccaggg tcccgttcca
     9361 gccgtcgcgc ccgcggtcgc cgccggcctc cgggcgcgag gttcagaggc cgacggcctg
     9421 atagagccgg cggacctcct cctggcgcag cggccggtac tcccccggcg ccagcccggt
     9481 gaggcgcacc gggccgacgg cggtccgcac cagccggcgc accggcgcgc cgacggcgtc
     9541 gagcaggcgg cggacctcgc ggttgcggcc ctccatcatg acgatctcaa ccatgcgtcg
     9601 gcgccccgag ctggccaccg cccgggcgcg ctccgccttg gcggggccgt cctccagggt
     9661 gactccggcg agcagtcggg agatcgcgtt tgccggcagc cgctccacct cggcgaggta
     9721 gcgtttctcc accccgaagc gggcgtgggt gagccggtag gcgagcgggc cgtcgttggt
     9781 gagcagcagc a
//
LOCUS       DASWDC010000358         3759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_496158, whole genome shotgun sequence.
ACCESSION   DASWDC010000358 DASWDC010000000
VERSION     DASWDC010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496158"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="VF995_11490"
     CDS             complement(<1..1328)
                     /locus_tag="VF995_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014690047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9344222.1"
                     /translation="MSQTRKFAGIPCGRWSKWAILGFWIVVVAVAGPLSGKLNNAQKN
                     DASQWLPKNAESTKVINLTKPFTSTDIVPAVVVYERSGTVTPADQAKAAADVKRFAAV
                     SHVSGRVVGPIPSKDGHAIEVFVPIKMGANGWNDLAPVVKDIRSIAHDSGGAVYVTGP
                     AGYGADFIKAFTGFDSTLLYITAAIVIVILLLTYRSPALWILPLSCVFVALTAAQAVI
                     YLLARHAGLTVNGQSAFILTVLVFGAGTDYALLLTARYREELRRHADRHQAMAVALGR
                     AGPALLASGSTVILSLLCLLVAELSSTKSLGPVMAIGVAVGLCAMLTLLPALLVIFGR
                     WMFWPRRPTLGSAEPTERGLWARIGARLARRPRLVWVVTAVVLGAMALGLTGLKASGL
                     QNRDAFRTTQNSVTGEQALDRHFAAGAGDSVQVIGRVFALWELRSAVANTP"
     gene            complement(1595..2785)
                     /locus_tag="VF995_11495"
     CDS             complement(1595..2785)
                     /locus_tag="VF995_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9344223.1"
                     /translation="MTTAPVPRRDVPPTPPLPRYGETSLADLASSLLSALDVPGFANP
                     LGIEPVRRVCLLVVDGLGWEALRARRAAAPLLYQASERGVALTAGFPSTTAASLASIG
                     TARPPSEHGLVGYTIALPGLDRPLNCLRWAAYGAGGYKDLRGRFVPEQLQPEPTAFER
                     AAADGVEVTLIGKRDYANSGFTRAALRGGRYVGTHSIGDDAASALRAVGAGRRTLVYA
                     YQPDLDRTGHVRGVGSEAWRLQLAHVDLLARQIAERLPGDAALVVTGDHGMVDLRPDQ
                     RIDVADEPGLTEGVRLLAGEARARHVHARKGADRDVLDAWRALLGHAMWVMPGEEAIE
                     AGWFGPNFPDRLRPRVGDVVAAAFGPVGVVQRAVDPTQAILTGHHGSLTSAEQLVPFL
                     DIRP"
     gene            2840..>3759
                     /locus_tag="VF995_11500"
     CDS             2840..>3759
                     /locus_tag="VF995_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344224.1"
                     /translation="MTEERRAQPTYNVLKTWAFDDWGLYGRGDELLSRALARHPRIGR
                     VFHVQPPLDPSELRSRFRDPRWEQDMEGQLRRLDGVEDDGVLLFAPTARADRQEAALD
                     GTIERLEREQAFARPLVLLHSLAHPFASDIARRLGGRGVTRVAVVRRDARAAYPPDAD
                     ERHALNDIYKVEIPDADLVWAGTSELVEEFRQLNPRTVRQPVGADPGLAAGRASRELA
                     DLPRPLVIYAGSMRAHLNVGLIRGTAERLGDASFVFVGPAAEFLAPLVSGVRNLHVIG
                     PRPYGLLPDYLAAADVAIAPHQVVPATRAVV"
BASE COUNT          554 a         1383 c         1336 g          486 t
ORIGIN      
        1 ggggtgttgg ccacggcgga ccgcagctcc cacaaagcga aaaccctgcc gatgacctgg
       61 accgagtcgc cggcgccggc cgcgaagtgg cggtcgagcg cctgctcgcc cgtcaccgag
      121 ttctgtgtgg tgcggaaggc atcccggttc tgcagcccgc tggccttcag cccggtcagg
      181 cccagcgcca tcgcgcccag cacgaccgcc gtcaccaccc acaccaggcg cggccggcgc
      241 gccaggcggg cgccgatccg cgcccacagg ccccgctcgg tcggctccgc cgaccccagc
      301 gtgggccgcc gcggccagaa catccagcgc ccgaagatga ccagcagcgc cggcagcagg
      361 gtcagcatgg cgcacaggcc caccgccacg ccgatcgcca tcaccgggcc caggctcttg
      421 gtggagctca gctcggcgac cagcaggcac agcaggctca agatcaccgt ggagccgctg
      481 gcaaggagcg ccggccccgc ccggccaagc gccaccgcca tggcctggtg ccggtcggcg
      541 tggcggcgca gctcctcgcg gtagcgggcg gtcagcagca gcgcgtagtc ggtgccggcg
      601 ccgaagacga gcacggtgag gatgaacgcg ctctggccgt tgacggtcag cccggcgtgg
      661 cgggcgagca ggtagatgac cgcctgcgcg gcggtcaggg cgacgaagac gcagctcagc
      721 ggcaggatcc acagcgcagg gctgcggtag gtcaggagca ggatgacgat gacgatcgcc
      781 gcggtgatgt acagcaacgt cgagtcaaac ccggtgaacg ccttgatgaa gtcggcgccg
      841 tagccggcgg ggccggtcac gtagaccgct ccgccgctgt catgggcgat cgaccggatg
      901 tccttgacca ccggggcgag gtcgttccag ccgttggcgc ccatcttgat cgggacgaac
      961 acctcgatgg cgtggccgtc cttggacggg atcgggccga cgacccggcc gctgacgtgg
     1021 ctgacggcgg cgaaccgctt gacgtcggcg gcggccttgg cctggtcggc cggggtgacc
     1081 gtgccggaac gctcgtagac gaccaccgcc gggacgatgt cggtggatgt gaatggcttg
     1141 gtcaggttga tgaccttggt ggactcggcg ttcttcggca accactggga cgcgtcgttt
     1201 ttctgcgcgt tgttcagctt gccggacagc ggtcccgcga ccgcgaccac cacgatccag
     1261 aagccgagga tcgcccactt ggaccagcgc ccgcagggaa tgccggcgaa cttgcgcgtt
     1321 tgactcatca gacctccaac atcaggctcg accagaccgg cagcatcacg gccaacgcct
     1381 gccaatcttc aaaccatcac ttaccaattg tcaatactca gctaccaatc atgaagcgca
     1441 tcggtaacct ggtcactgct atgacggatc ccaccgaaag aacgtgacag cgagcgaccc
     1501 gctgatgctg ccatgccccc ccgacaacct aagcaggcgc gccgcatccc gccacccgac
     1561 ccgagcaccc ggcgacgtat cttccccaag tcacctacgg gcggatatcc aggaatggca
     1621 ccaactgctc cgcgctggtc agggagccgt gatggccggt gaggatcgcc tgggtgggat
     1681 cgacggcgcg ctgcaccacc ccgaccgggc cgaaggcggc cgcgaccacg tcgccgaccc
     1741 ggggccgcag ccggtcgggg aagttggggc cgaaccagcc ggcctcgatc gcctcctcgc
     1801 ccggcatcac ccacatggcg tggccgagca gcgcccgcca ggcgtcgagc acgtcccggt
     1861 ccgcgccctt cctggcgtgc acgtgccggg cgcgcgcctc gccggcgagc agccgcaccc
     1921 cctcggtcag ccccggctcg tcggcgacgt cgatccgctg gtcgggtcgc agatcgacca
     1981 tgccgtggtc gccggtcacc accagcgccg cgtcgccggg caggcgctcg gcgatctgcc
     2041 gggcgagcag gtcgacgtgg gcgagctgca gccgccacgc ctcgctgccg acgccgcgga
     2101 cgtgcccggt gcggtccagg tccggctggt aggcgtagac gagcgtgcgc cgcccggcgc
     2161 cgaccgctcg cagggcgctg gcggcgtcgt cgccgatcga gtgggtgccg acgtagcgcc
     2221 cgccgcgcag cgccgccctg gtgaacccgg agttggcgta gtcgcgcttg ccgatcaggg
     2281 tgacctcgac gccgtcggcg gccgcccgct cgaaggcggt cggctccggc tgcagctgtt
     2341 ccggcacgaa gcggccgcgc aggtccttgt agccgcccgc tccgtacgcc gcccagcgca
     2401 ggcagttgag cggccggtcc aggccgggaa gggcgatggt gtagccgacc aggccatgct
     2461 cggacggcgg ccgggcggtg ccgatcgagg ccaggctggc cgcggtggtc gaggggaacc
     2521 cggccgtcag cgccacgccg cgctcggacg cctggtacag cagcggcgcc gccgcccggc
     2581 gggcccgcag ggcctcccag ccgagcccgt cgaccaccaa caggcagacc cgccggaccg
     2641 gctcgatccc gagcgggttg gcgaacccgg gcacgtccag cgcgctcagc agcgagctgg
     2701 ccaggtcggc gagcgacgtc tccccgtagc ggggaagcgg cggcgttggc gggacgtcgc
     2761 gccgtggtac tggagcggtg gtcataatag gcagcctacc cttgccttga gccgagctgg
     2821 gacggagcgg agggcctgag tgaccgagga gcggcgcgcg cagccgacct ataacgtgct
     2881 gaagacctgg gcgtttgatg actggggctt gtacggccgc ggtgacgagc tgctcagccg
     2941 ggccttggcg cgccacccgc ggatcggccg ggtcttccac gtgcagcctc cactcgaccc
     3001 gagcgagctg cgcagccgct tccgcgaccc gcgctgggag caggacatgg aggggcagct
     3061 ccgccgcctc gacggcgtcg aggacgacgg cgtgctgctg ttcgcgccga ccgcccgggc
     3121 cgaccgccag gaggcggccc tcgacggcac catcgagcgg ctggaacgcg agcaggcctt
     3181 cgcccggccg ctggtgctgc tgcactcgct ggcccacccg ttcgcctcgg acatcgcccg
     3241 ccggctcggc ggccggggcg tcacccgggt ggcggtggtg cgccgcgacg ccagggccgc
     3301 ctacccaccc gacgccgacg agcgccacgc cctcaacgac atctacaagg tcgagatccc
     3361 cgacgcggac ctggtctggg ccgggaccag cgagctggtc gaggaattca ggcagctcaa
     3421 cccccgcacg gtgcgccagc cggtcggcgc cgacccgggg ctcgcggctg gccgggcctc
     3481 gcgggagctg gccgacctgc cgcgcccgct ggtgatctac gccggttcga tgcgcgcgca
     3541 cctcaacgtc gggctgatcc gcggcaccgc cgagcggctc ggcgacgcca gcttcgtgtt
     3601 cgtcggaccc gccgccgagt tcctggcgcc gctggtctcc ggcgtccgca acctccacgt
     3661 catcggcccg cgcccctacg ggctgctgcc cgactacctg gccgccgccg acgtggcgat
     3721 cgcgccgcac caggtcgtcc cggcgacccg cgcggtcgt
//
LOCUS       DASWDC010000359         5827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1160611, whole genome shotgun sequence.
ACCESSION   DASWDC010000359 DASWDC010000000
VERSION     DASWDC010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160611"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VF995_11505"
     CDS             <1..731
                     /locus_tag="VF995_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9344225.1"
                     /translation="REVLLTTTAGDVACMIAEPIQGVGGFATPPDGFFGAMKEVLDES
                     GILFISDEVQTGWGRTGEHFWGIQAHGVVPDAMTFAKGLGNGLAIGGVVARGELVDAV
                     AANSISTFGGNPLATRGALAVLDYLRDHDLQANARTVGARLFAGLRERAGHLPIVGEI
                     RGKGLMIGVELVEPGGRTPRPRAAARVMEETRKGGLLIGKGGLYGNVLRIAPPLTVSA
                     AEADEGLEILTAALSAASQEVAST"
     gene            728..2242
                     /locus_tag="VF995_11510"
     CDS             728..2242
                     /locus_tag="VF995_11510"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX9344226.1"
                     /translation="MTDTSTEPARITHWIGGRRWDGTAERQSEVYDPATGRLSGRVDF
                     AGAAEVGAAVEAAAGAFPAWRDASLAKRASVLFAFRELVNRHRAELAALITAEHGKVQ
                     SDAEGEVTRGLEVVEFACGIPHLLKGGYSENVSTKVDSYAIRQPLGVVCGITPFNFPA
                     MVPMWMFPLAIACGNTFVLKPSEKDPSASIRIAELWAEAGLPDGVFNVVHGDKAAVDA
                     LLDHPGIRAVSFVGSTPIARYVYQRGTSHGKRVQALGGAKNHMVVLPDADLDLAADAA
                     VNAGFGSAGERCMAISVVVAVEPAADDLVGKIADRMAGLRVGPGCDAAAQMGPLVTGP
                     HRDKVAAYLDAGTASGAELVVDGRKHQIDGEPSGYWLGPSLFDRVTPEMGVYRDEIFG
                     PVLSVVRAASYDDALDMVNANPYGNGTAIFTNDGGAARRFQNEVQVGMVGINVPIPVP
                     MAYYSFGGWKQSLFGDSHVHGIEGVHFYTHGKVVTARWLDPSHGGLNLGFPQNT"
     gene            complement(2265..4118)
                     /gene="ilvD"
                     /locus_tag="VF995_11515"
     CDS             complement(2265..4118)
                     /gene="ilvD"
                     /locus_tag="VF995_11515"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017533761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9344227.1"
                     /translation="MPPLRSRTVTHGRNMAGARALWRATGMTTADFGKPIVAVANSFT
                     QFVPGHVHLRDVGRIVADAVAAAGGVAREFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     AVEYMANAHCADALVCVSNCDKITPGMLMAALRLNIPAVFVSGGPMEAGKVVASDGVA
                     RRSLDLIDAMIASADDRTSDEQLEELESAACPTCGSCSGMFTANSMNCLVEALGLGLP
                     GNGSVLATHTARRRLFADAGTTVMALAKRWYDEDDESALPRSVASRDAFENAMALDIA
                     MGGSTNTILHLLAAAQEAGVGFGLKDIDELSRRVPCVCKVAPSSAFHLEDVHRAGGIP
                     AILGELDRGGLLNEGVHMVHSGSLREYLDRWDVRGAAPNAEALELYHAAPGGVRTTEP
                     FSQSTRWESLDTDAETGCVRSVRHAYTTDGGLAVLWGNLAPEGCIIKTAGVDPSLWRF
                     AGPARVFESQEAAVEGILNNRIQPGDVVVIRYEGPRGGPGMQEMLYPTAFLKGKGLGA
                     KCALITDGRFSGGTSGLSVCHISPEAAASGTIGLIEEGDTIAIDLPSRSLELRIDADE
                     LRARRERLLTDLGGWRPRDRDRPVSAALQAYAALATSASTGAVRDLGLLRR"
     gene            4337..5134
                     /locus_tag="VF995_11520"
     CDS             4337..5134
                     /locus_tag="VF995_11520"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9344228.1"
                     /translation="MRRVVVANQKGGVGKTTTVVNLGAALSELGRRVLVIDLDPQANA
                     STWLGSVDARALYEVFENGAPLAGAVRPTDVKGLELVPSSSWMTRAERNAATQAGAEM
                     ILRQAVDELPRDRWDVILMDCPPALGLLSYSALAACDEVLVPVQTRVMPLAGLVALTQ
                     TIETVRLRLNRGLRLSAVLPSQVDIRTSLSKEIVEVLRERFGELVLNTMIRESVRIAE
                     APGHRQPVTVYAPESSAANDYRAAALEFDARSAAHGETEADGRVQRA"
     gene            5094..5720
                     /locus_tag="VF995_11525"
     CDS             5094..5720
                     /locus_tag="VF995_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344229.1"
                     /translation="MARPRRMGAYNALEDLPARPSSRPTGPALLRPWLEPVPDPPPEP
                     PARAPRRPAGRNALDDLLPGPGQGPDDDGFDPGSGPAPARPWLEPVPDLPEAPPPRRG
                     RRVLGRNALDELLPGPGGSSSALGAGLGVGAGPAEDGPKARITLRLPAGLLEAVRDAA
                     THLAGPPNYLTIAALAERALRAELDRLGTDHNHGRPFPPRPGSPGARR"
BASE COUNT          810 a         2171 c         2030 g          816 t
ORIGIN      
        1 tgcgcgaggt gctgctgacc accaccgctg gggacgtcgc ctgcatgatc gccgagccga
       61 tccagggcgt cggcgggttc gcgaccccgc cggacgggtt cttcggcgcg atgaaggagg
      121 tcctggacga gtccggcatc ctgttcatct ccgatgaggt ccagaccggc tggggccgca
      181 ccggcgagca cttctggggc atccaggccc acggcgtggt ccccgacgcg atgaccttcg
      241 ccaaggggct cggcaacggc ctggcgatcg gcggggtggt ggcccgcggc gagctggtcg
      301 acgcggtcgc cgccaactcg atctcgacct tcggcggcaa cccgctggcc acccgcgggg
      361 cgctggcggt gctcgactac ctgcgcgacc acgatctgca ggccaacgcc cgcaccgtcg
      421 gcgcacgcct gttcgccggg ctgcgggagc gcgccgggca cctcccgatc gtcggcgaga
      481 tccgcggcaa gggcctgatg atcggcgtcg agctggtcga gccgggcggc aggacccccc
      541 gcccgcgcgc cgccgcccgg gtgatggagg agacccgcaa gggcggcctg ctgatcggca
      601 agggcggcct gtacggcaac gtgctgcgca tcgctccgcc gctgactgtc agtgccgccg
      661 aggccgacga aggccttgag atcctcaccg cggccctgtc cgccgcaagc caggaggtcg
      721 cttcgacatg accgacacca gcaccgaacc cgcccgcatc acccactgga tcggcggccg
      781 ccgctgggac ggcaccgccg agcggcagag cgaggtctac gaccccgcca ccggccggct
      841 cagcggccgg gtcgacttcg ctggcgccgc cgaggtcggc gccgccgtcg aggctgccgc
      901 cggcgcgttc cccgcctggc gggacgcctc cctggccaag cgcgccagcg tcctgttcgc
      961 cttccgcgag ctggtcaacc gccaccgggc cgagctggcc gcgctgatca ccgccgagca
     1021 cggcaaggtc cagtccgacg ccgaaggcga ggtgacccgc ggccttgagg tggtcgagtt
     1081 cgcctgcggc atcccgcacc tgctcaaggg cggctactcc gagaacgtct ccaccaaggt
     1141 cgacagctac gccatccgcc agccgctcgg cgtggtctgt gggatcaccc cgttcaactt
     1201 ccccgcgatg gtcccgatgt ggatgtttcc tctggcgatc gcctgcggga acaccttcgt
     1261 cctcaagccg agcgagaagg acccgtcggc ctccatccgc atcgccgagc tgtgggccga
     1321 ggcgggcctg cccgacgggg tcttcaacgt cgtccatggc gacaaggccg ccgtcgacgc
     1381 cctcctcgac caccccggaa tccgcgcggt cagcttcgtc ggctccactc cgatcgcccg
     1441 ctacgtctac cagcgcggca ccagccacgg caagcgcgtc caggccctcg gcggcgccaa
     1501 gaaccacatg gtggtgctgc ccgacgccga cctcgacctc gccgccgacg ccgccgtcaa
     1561 cgccggcttc ggctcggcgg gggagcgctg catggcgatc tcggtggtgg tcgccgtcga
     1621 gcccgcggcc gacgacctgg tcggcaagat cgccgaccgt atggcgggcc tgcgggtcgg
     1681 gcccggctgc gacgccgcag cccagatggg cccgctggtc accggccccc accgcgacaa
     1741 ggtcgccgcc tacctggatg ccggcaccgc ctccggcgcc gagctggtgg tcgacggccg
     1801 caagcaccag atcgacggcg agccgagcgg ctactggctc ggccccagcc tgttcgaccg
     1861 ggtcactccc gagatgggcg tctaccgcga cgagatcttc ggcccggtcc tgtccgtcgt
     1921 ccgcgccgcc agctacgacg acgcactgga catggtcaac gccaacccct acggcaacgg
     1981 caccgccatc ttcaccaacg acggcggcgc cgcccgccgc ttccagaacg aagtccaggt
     2041 cggcatggtc ggcatcaacg tcccgatccc ggtcccgatg gcctactact ccttcggcgg
     2101 ctggaagcag tccctcttcg gcgacagcca cgtccacggc atcgagggcg tccacttcta
     2161 cacccacggc aaggtcgtca ccgcccgctg gctcgacccc agccacggcg gcctcaacct
     2221 cggcttcccg cagaacacct agccctgggg cgtccggttc agcgtcagcg ccgcagcagg
     2281 cccaggtcgc ggaccgcgcc ggtggaggcg gaggttgcca gcgccgcgta ggcctgcagc
     2341 gcggcgctga ccgggcggtc gcggtcgcgg gggcgccagc cgcccaggtc ggtgaggagg
     2401 cgttcgcggc gggcgcgcag ctcgtcggcg tcgatccgca gctccaggga gcggcttggg
     2461 aggtcgatgg cgatcgtgtc gccctcctcg atcagcccga tggtgccgct ggcggctgcc
     2521 tccggggaga tgtggcagac cgacaggccg gaggtgccgc cggagaagcg gccgtcggtg
     2581 atcagggcgc acttggcgcc gaggcccttg cccttcagga aggcggtcgg gtagagcatc
     2641 tcctgcatgc ccgggccgcc gcgggggccc tcgtagcgga tcaccaccac gtcgccgggc
     2701 tggatgcggt tgttgaggat cccctcgacg gcggcctcct ggctttcgaa cacccgcgcc
     2761 gggccggcga agcgccacaa cgacgggtcg accccggccg tcttgatgat gcagccctcc
     2821 ggcgcgaggt tgccccacag caccgccagg ccgccgtcgg tggtgtaggc gtggcgcacc
     2881 gagcggacgc agcccgtctc ggcgtcggtg tccagcgact cccagcgggt ggactggctg
     2941 aacggctcgg tggtgcgcac cccgcccggg gcggcgtggt acagctccag cgcctcagcg
     3001 ttcggggcgg cgccacggac gtcccagcgg tccaggtact cccgcagcga tcctgaatgg
     3061 accatgtgca cgccctcgtt gagcaggccg ccgcggtcca gctccccgag gatcgccggg
     3121 atcccgccgg cgcggtggac gtcctccagg tggaacgcgg agctgggcgc caccttgcag
     3181 acgcacggca cccgccgcga cagctcgtcg atgtccttca ggccgaagcc gactccggcc
     3241 tcctgcgcgg cggccagcag gtgcaggatc gtgttggtcg acccgcccat ggcgatgtcg
     3301 agcgccatcg cgttctcgaa cgcgtcgcgg ctggcgacgc tgcggggcag cgccgactcg
     3361 tcatcctcgt cgtaccagcg cttcgccagc gccatcacgg tggtgcccgc gtcggcgaac
     3421 agccgccgcc gggcggtgtg ggtcgccagc accgagccgt tgcccggcag gccgaggccg
     3481 agcgcctcca ccaggcagtt catcgagttc gcggtgaaca tccccgagca cgacccgcag
     3541 gtcgggcagg cggcgctctc cagctcctcc agctgctcgt cggaggtccg gtcgtccgcc
     3601 gaggcgatca tcgcgtcgat caggtcaagg ctgcggcgcg cgaccccgtc gctggcgacg
     3661 accttgccgg cctccatcgg cccgcctgac acgaacaccg ccgggatgtt cagccgcagc
     3721 gccgccatca gcatccccgg ggtgatcttg tcgcagttgg agacgcacac cagcgcgtcg
     3781 gcgcaatggg cgttggccat gtactcgacc gcgtcggcga tcagctcacg gctcggcagc
     3841 gaatacagca tcccgccgtg ccccatggcg atcccgtcgt cgaccgcgat ggtgttgaac
     3901 tcccgcgcga ccccacccgc cgccgccacc gcgtcggcca cgatccggcc gacgtcccgc
     3961 aggtggacgt gcccggggac gaactgggtg aagctgttcg cgaccgccac gatcggcttg
     4021 ccgaaatcag ccgtggtcat ccccgtcgcc cgccacagcg cacgggcacc cgccatattc
     4081 cgtccatggg tcacggtccg ggaccgcagt ggtggcatca cgcctccaag tgaaaactcg
     4141 acgttgacga aaaagctgac ggccattgtt gcactgcagc gccgcgatcc tcgaatcgtc
     4201 gaccaagccg cccggcctgc ccggattgga cctgtggtgg gagcggtgcg gtatgttgcc
     4261 cagcttccac cagtccgcgc tgcttctgtt gtaggctgcc gccttgatac tgaatgcctc
     4321 tcaacaggtg gtgcccatgc gacgtgtcgt agtcgcgaac cagaaaggcg gcgtcggcaa
     4381 gacgacgacc gtggtgaacc tcggcgccgc cctttccgag ctgggcaggc gggtgctggt
     4441 gatcgacctg gacccgcagg ccaacgccag tacctggctc ggctcggtgg acgcccgcgc
     4501 gctctacgag gtcttcgaga acggcgcccc gctggcgggg gcggtgcggc ccaccgatgt
     4561 gaagggtctt gagctggtgc cgtcgtcgtc gtggatgacc cgggcggagc gcaacgccgc
     4621 cacccaggcg ggcgcggaga tgatcctgcg ccaggcggtg gacgagctgc cgcgcgaccg
     4681 ctgggacgtg atcctgatgg actgcccgcc ggcgctcggg ctgctgtcgt attcggcgct
     4741 ggccgcctgc gacgaggtgc tggtgccggt gcagacgcgg gtgatgccgc tggcgggact
     4801 ggtggcgctg acgcagacaa tcgaaacggt gcggctgcgc ctcaaccgcg ggctgcggct
     4861 gtcggcggtg ctgcccagcc aggtggacat ccgcacctcg ctgtccaagg agatcgtcga
     4921 ggtgctgcgg gaacgcttcg gcgagctggt cctgaacacg atgatccgcg agagcgtccg
     4981 catcgccgag gcgccgggcc accggcagcc ggtcaccgtc tacgcccccg aaagttcagc
     5041 ggcgaacgac taccgcgccg ccgcgctcga gttcgacgcg aggagtgcag ctcatggcga
     5101 gaccgaggcg gatgggcgcg tacaacgcgc ttgaggacct ccccgcccga ccctcctccc
     5161 gcccgacggg tccggcgctg ctgcgtccct ggctggagcc ggtccccgac ccgcccccgg
     5221 agccgcctgc ccgcgcgccc cggcgaccag ccgggcgcaa cgccctcgac gacctgctgc
     5281 ccggccccgg ccagggaccc gacgacgacg gcttcgaccc gggctcgggt cccgcgccgg
     5341 cccggccgtg gctggagccg gtccccgact tgccggaggc cccgccgccg cgccgtggcc
     5401 ggcgggtcct cggccgcaac gcccttgatg agctgctgcc cggcccgggt ggcagctcat
     5461 ctgcccttgg ggccggtctt ggggttggcg ccgggccagc ggaggacggc cccaaggcgc
     5521 ggatcaccct acggctcccg gcggggctgc tggaagcggt ccgcgacgcc gcgacgcacc
     5581 tggctgggcc gcccaactac ctgaccatcg ccgccctcgc cgagcgggcg ctgcgcgccg
     5641 agctggatcg cctcggcacc gaccacaacc acggccggcc gttcccaccc cggccaggca
     5701 gcccaggcgc acgccgctag ccgggtgttt cgcacatgag agttggttgg ggtggagagg
     5761 tcatgatggc ctccgatcag tcagctcgcc cagcgtgggc gggtcgggtc aaccttgagc
     5821 agctcca
//
LOCUS       DASWDC010000360         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1585105, whole genome shotgun sequence.
ACCESSION   DASWDC010000360 DASWDC010000000
VERSION     DASWDC010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585105"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VF995_11530"
     CDS             complement(<1..298)
                     /locus_tag="VF995_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344230.1"
                     /translation="MAADNTMPAVLQRRQDGNSAVPTGKTLDIESGGALAIAGTDRTA
                     VLATAPAAIAAGYKIARGQFTTISASDTVVTGLATVVSAVACLDSDPGDNPMLAN"
     gene            complement(405..848)
                     /locus_tag="VF995_11535"
     CDS             complement(405..848)
                     /locus_tag="VF995_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344231.1"
                     /translation="MRNLLKACKITRVANSAVAGTTDVTSSVLDMAGFDGVIYVALLG
                     DVTVNSVLTLTAKENTANSTSTPTPTSITGGATAAYTATATDSDNKALVVDVNRPLRQ
                     FQFCILSRTAANAVVDGIIAIQYKAHLKPTVQDVSVLAQAFAVGS"
     gene            complement(910..2196)
                     /locus_tag="VF995_11540"
     CDS             complement(910..2196)
                     /locus_tag="VF995_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX9344232.1"
                     /translation="MTPKEMAEKRIALTREAQGLIDKAANEKRSLAAAEDERIDKIFA
                     EVDGLRTQIDAAEKSETRTKRLDDEKRWADQTAGRRTAAVEPVALGGGNKPAVWNATD
                     EYRSAYSRFLRGGARVLGADEYRLLQGPEELRALQADADVAGGFLVAPQQMVNDLVKF
                     KDNLVFMRGLATKYTVNGAGSLGAPSLDADPADSNWTAEILTGAEDSTMAFGKRELFP
                     KPLAKRIKVSRTLLNRTANGAESLVRDRLGYKQAVTEEQAFLTGTGAGQPLGVFVASA
                     SGISTGRDVSTDNTTTNLTADGLINAKFSLKAPYMASPSVRWIFHRTAVRNIRKLKDG
                     NGQYLWAAGLGGKPDTILEVSYAMSEYAPSTFTTGLYVGIIGDFSFYWIADALNLQIQ
                     RLDELYAETNQVGFIGRSETDGMPVLEEAFARVKLA"
     gene            complement(2379..>3299)
                     /locus_tag="VF995_11545"
     CDS             complement(2379..>3299)
                     /locus_tag="VF995_11545"
                     /inference="COORDINATES: protein motif:HMM:NF016471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEX9344233.1"
                     /translation="RFFSNAELRVIDGPSGRPRIVGYAAVFNARSGDLGGFREEIAPG
                     AFTDALAADGDVLALVEHNPGLILGRRSSSTLSLQQDARGLRVEIDPPNTTVGNDAIE
                     NVRRGDIKGMSFRFPRGAKDSWRKDGSDSVRTLHKVGLLEVTLTSIPAYDATSVSLRA
                     LDDRAEAELRSYYEAQIAALTPPPKAPEPPAPAATPAAPDLGLARRRVQLLAAGLSAG
                     EARAMMQMDDKSLIYCSRGAIIYLRQAFDGLQSVIDCTGAVAGALTPADRTAIDDCIG
                     AVIDLLAKAKMARQSLQALGAQDDAGAAAA"
BASE COUNT          415 a         1028 c         1282 g          574 t
ORIGIN      
        1 cgttggccag catcgggttg tcgccggggt cggagtcgag gcaggcgacg gccgagacga
       61 ccgtcgccag gccggtgacg accgtgtcgc tggcgctgat ggtggtgaac tggccgcggg
      121 cgatcttgta gccggcggcg atggccgcgg gggcggtcgc caggacggcg gtgcggtcgg
      181 tgccggcgat ggccagggcg ccgccggact cgatgtcgag ggtcttgccg gtggggacgg
      241 cggaattgcc gtcctggcgg cgttggagga cggcgggcat ggtgttgtcg gcagccattt
      301 cttgctcctg ttgaggttcg gaaagaagcg gacgcggggc gcggcggcat cagcctgccg
      361 ccgcgccccc tcccggttga accgcgtcgt gaatggttcg gcggttacga gccgaccgcg
      421 aacgcctggg ccaggacgga gacgtcctgc accgtcggct tgaggtgggc cttgtactgg
      481 atggcgatga tgccatcgac caccgcgttg gcggcggtgc ggctgaggat gcagaactgg
      541 aactgcctaa gcggccggtt cacgtccacc accagcgcct tgttgtcgga atcggtggcg
      601 gtggcggtgt aggccgcggt cgccccgccg gtgatcgagg tgggggtcgg ggtgctggtg
      661 ctgttggccg tgttctcctt ggccgtgagg gtgagaactg agttgacggt gacgtcgccg
      721 agcagggcga cgtagatcac gccgtcgaac ccggccatgt ccagcacgct gctggtcacg
      781 tcggtggtgc ccgcgaccgc cgagttggcg acgcgggtga ttttgcaggc tttgagaaga
      841 ttccgcatgg gaaggtctcc ggttttcgtt ttgaagaaga aggagctcgc cgcgcgaagg
      901 gcttggcgat tacgcgagct tgacgcgggc gaaggcctct tcgagcacgg gcatgccgtc
      961 ggtctcggag cggccgatga agccgacctg gttggtctcg gcgtacagct catccagccg
     1021 ctggatctgc aggttcaggg cgtcggcgat ccagtagaag ctgaagtcgc cgatgatccc
     1081 gacgtacagc ccggtggtga acgtggacgg cgcgtactcg ctcatcgcgt acgacacctc
     1141 gaggatggtg tcgggcttgc cgcccaggcc ggcggcccag aggtactggc cgttgccgtc
     1201 cttgagcttg cggatattgc gcacggccgt gcggtggaag atccagcgca cggaggggct
     1261 ggccatgtac ggggccttga ggctgaactt ggcgttgatc agcccgtcgg cggtcaggtt
     1321 ggtggtcgtg ttgtcggtgc tgacgtcgcg gccggtgctg atgccgctgg cggaggcgac
     1381 gaacacgccc agcggctggc cggccccggt gccggtcagg aacgcctgct cctcggtgac
     1441 cgcctgcttg tagcccaggc ggtcgcggac cagcgactcg gcgccgttgg cggtgcggtt
     1501 gagcagcgtg cgcgagacct tgatccgctt ggccagcggc ttggggaaca gctcgcgctt
     1561 gccgaaggcc atggtcgagt cctcggcccc ggtcaggatc tcggccgtcc agttggaatc
     1621 ggccgggtcg gcgtcgagcg acggcgcgcc caggctgccg gcgccgttca ccgtgtattt
     1681 ggtggccagg ccgcgcatga agaccaggtt gtccttgaac ttgaccaggt cgttgaccat
     1741 ctgctgcggg gcgaccagga agccgccggc gacgtcggcg tcggcctgga gggcgcggag
     1801 ctcctcgggg ccctggagca ggcggtattc gtccgccccc aggacccgcg ccccgccgcg
     1861 gaggaaccgg ctgtaggcgc tgcggtattc gtcggtggcg ttccagacgg cgggcttgtt
     1921 gccgccgccc agggccaccg gctcgaccgc ggccgtgcgg cggccggcgg tctggtccgc
     1981 ccagcgcttc tcgtcgtcga gccgcttggt ccgggtctcg ctcttctccg cggcgtcgat
     2041 ctgggtgcgg agcccgtcca cctcggcgaa gatcttgtcg atccgctcgt cctcggcggc
     2101 ggcgaggctg cgcttctcgt tcgccgcctt gtcgatcagg ccctgcgcct cgcgggtgag
     2161 cgcgatccgc ttctcggcca tttctttggg agtcatggtc agttccttgg gttgaaaaaa
     2221 cgggaaggga aagggacgcg agcggcgccg ccttcgccga tggcgcgggc gctggcgctg
     2281 tcgcgcggcc ggtggccgcg gcaccgatgg tgccgcgaat cggctcgcgc cggttttggt
     2341 tcggttgatt cgattggttc gttgcgttgc ccgggcggct acgcggcggc ggcgccggca
     2401 tcgtcctggg cccccagggc ctggagcgac tgccgggcca tcttggcctt ggccagcaga
     2461 tcgatcaccg cgccgatgca gtcgtcgatg gcggtccggt ccgcgggggt gagggccccg
     2521 gcgacggcgc cggtgcagtc gatgacgctt tgcaggccgt cgaacgcctg gcgcaggtaa
     2581 atgatcgcgc cgcgggagca atagatcagc gacttgtcgt ccatctgcat catcgcccgg
     2641 gcctcgccgg cggacagccc ggcggcgagc aactgcaccc ggcgccgggc taggcccagg
     2701 tccggggcgg cgggcgtcgc ggccggcgcg ggcggctcgg gcgccttggg cggcggggtc
     2761 agggcggcga tctgcgcctc gtagtaggag cgaagctcgg cctctgctcg atcgtccagg
     2821 gcccgcaggg agacgctggt ggcgtcgtag gcggggatgc tggtcagggt cacctcgagc
     2881 agtcccacct tgtgcagcgt gcgcacgctg tcggagccgt ccttgcgcca actgtccttc
     2941 gccccgcgcg ggaaccggaa gctcatcccc ttgatgtcgc cgcggcggac gttctcgatc
     3001 gcgtcgttgc cgacggtcgt gttgggcggg tcgatctcga cgcggaggcc gcgcgcgtcc
     3061 tgctggagcg agagggtgct cgagctgcgc cgtccgagga tcaggcccgg gttgtgctcg
     3121 accagggcca gcacgtcgcc gtcggcggcg agggcgtcgg tgaaggcgcc gggggcaatc
     3181 tcctcgcgga agccccccag gtcgccgctg cgggcgttga agacggcggc atagccgacg
     3241 atccgcggac ggccgctcgg gccgtcgatg acccgcagct cggcgttgga gaagaagcg
//
LOCUS       DASWDC010000361         7327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1161095, whole genome shotgun sequence.
ACCESSION   DASWDC010000361 DASWDC010000000
VERSION     DASWDC010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161095"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="VF995_11550"
     CDS             complement(<1..1441)
                     /locus_tag="VF995_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344234.1"
                     /translation="MSTLFTANGDGAGRRTRPPERRRPGRFRPTRAGLRNVWEYDDAV
                     LHFAGGRVIFRGPNGSGKSNALALLFPFLLDGRAGATYMDPHGGGRNLKSLLLCQRRG
                     DGPRRYEFDSRVGYVWLELRRDTPAGPRWVTIGAGARATTSRDAETWFFVTSRRVGVD
                     LDLVPGGVPLTRGGLIAALGDHLPPPVDAEITDPVDEPAGNRPERPDPEPEDDEWPAD
                     ELEGDVEAPAPGRRGRRRLVSACVFDTIDEYQAAVDRALFGMGVDRLRKLVNLVLVLR
                     RPHLAKQLDLDGLSASLSEALTPLAGDVIETVAAAFEDLDRIRGRLKELRAAVAAVNE
                     ALPWYRDYLRAEARARALALAEADRAAQAARRQHRQADREQVAAEAFVLELEQARETA
                     RVEQRDATGSLRGLEQSDEYRALLTIDDLAALVASRQAATTRAEEALAEAAADAEQAA
                     ADHAEAETAVAAAAGTLAERARALAAAATA"
     gene            complement(1441..2694)
                     /locus_tag="VF995_11555"
     CDS             complement(1441..2694)
                     /locus_tag="VF995_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02678 family protein"
                     /protein_id="HEX9344235.1"
                     /translation="MSPARYASMLDPVELEDFQEAARTLLAHPLVTATYPNPEALPKV
                     RRWETTLRNEFERLTRWRLEVAPSCARLVQRRVEPSPRRPAMVTSAGGERSPRPFGRL
                     DYVLLCLVLAALHGEGGQTTIRRLVEGAQRLRAGDERLPVDFGRIEYRRAFVHAVGYL
                     ERLGVLRTAPDGETIAFIDDEEADALYTVDHDAARRLLGPTPAWIEPERGLAQFCEER
                     YPPGADTTDARLRHRLSRRLLDEPAVYYDELPVDERDHLAERRARLVGDLARLTGCEV
                     EVRAEGLALIDSAGQPRLNDQPFPGQGIVNNLALLWADELADAEAGQGQVAGQLPVGR
                     RIPPEAVEATWAAMVRQHGDRFTVAYQADPDALRVAALALLERLGLAVAPGPEEPPGT
                     VVLRPLAARFRPGEGGRPARRRRRR"
     gene            complement(2691..4361)
                     /locus_tag="VF995_11560"
     CDS             complement(2691..4361)
                     /locus_tag="VF995_11560"
                     /inference="COORDINATES: protein motif:HMM:NF021197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02677 family protein"
                     /protein_id="HEX9344236.1"
                     /translation="MANNPLVTTELPAVAMDGQSGNGHAPEAPTPEPAAVAVAGQTRA
                     APGEVPPAGARAFHERLLEAGERDRLRALLVGTTERAERYLAILSAMVAARAAYRPQL
                     RTEELLAMLPASALELDFDKLIEVLDALKGMGCVDWTPDTRHVTRKEDMYRRRAQWYL
                     TAVGVAAFEAAQAVLAAVEAAGGLRARLFEGLRRNLELLLAAVRAGDAGGVYGLLRSL
                     DTDLRDLTRSAQDFSTKLAELTRGSELDAAVFLDCKELLLTYLSTFSDDLYRYRRRMA
                     ATAAEITDEGVDRLVELAAAGDDGAAAALGLGRDLRASWQSRWTGLERWLSASDGRPS
                     GLDELFSATTQAVRDVLGQIRRLSEAESRPVNRTSELLALARWFHRCDGAAEAHALFD
                     AAFGLGRTPRVSGLPPDPTATGPRASWWSAEPVEIAVSLRERGQHSTPGKTARAVDFS
                     AQREALAADAAAAERERAEAATRLASAPLAGRILSRAELELLLSLVDECLLGVLGLPG
                     ADGADGAAVLAAAEGVTVRARPDPAGCVIRCVDGDLVFDGLAVEVVPR"
     gene            complement(4636..5205)
                     /locus_tag="VF995_11565"
     CDS             complement(4636..5205)
                     /locus_tag="VF995_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9344237.1"
                     /translation="MNEYHLKVCASAEWAETVERVLPWVLEGRQLGDQVLEVGAGPGV
                     VTDALRPRVPRLVAVELDQQLAASLGQRLSGAGVEVVRADATRLPFRSRAFSTVASFT
                     MLHHVPSVALQDRMLGELCRVLRPGGLLVGTDGMDTPSRREAHIDDLFVPALPEELPG
                     RLRAAGFATAAVDVDADGDRFRFVAASPR"
     gene            5356..5751
                     /locus_tag="VF995_11570"
     CDS             5356..5751
                     /locus_tag="VF995_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX9344238.1"
                     /translation="MNGIPDSHADILDKAGLAYLATAGPKGEPQVSPVWFGWDGRYLR
                     VSHTPDRQKYRNVRRDPRVALAFNDPDDLYRHIEIRGRVERIDPDPDHAFINAMAKKY
                     TGVDVYEAAPPSQERVILVIAPEHANTMG"
     gene            5806..6066
                     /locus_tag="VF995_11575"
     CDS             5806..6066
                     /locus_tag="VF995_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9344239.1"
                     /translation="MAESVMEERVVMASRFKERCLALLDQVAETKVPVVVTKHGKPVA
                     KLVPIDEPVAKPMMGSVRLLVEDDESYFSTGESWVAEGPHGE"
     gene            6063..6464
                     /locus_tag="VF995_11580"
     CDS             6063..6464
                     /locus_tag="VF995_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007101276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9344240.1"
                     /translation="MILLDTHALLWWAAGSDRLSPGAARAIARADTILVSPISCWEVA
                     TLVGKGRIALDRDVYVWVLDLFSLDEVELAPLTPQMAVAAALLGTAGFHGDPADRFLY
                     ATALEHSVPLVTKDESIRSYAEKAGDLRTIW"
     gene            7079..>7327
                     /locus_tag="VF995_11585"
     CDS             7079..>7327
                     /locus_tag="VF995_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344241.1"
                     /translation="MGEFRRLDDQLGSAGLRQAVLVHLRLVQALRGTGRTEEAESALG
                     SIEAEFAQFAGWLAFDACDYRAAERYLLIAYKAADSEGD"
BASE COUNT          980 a         2729 c         2658 g          960 t
ORIGIN      
        1 cggcggtcgc ggcggcggcg agggcgcggg cccgttcggc cagggtccca gcggccgccg
       61 cgaccgccgt ctcggcctcc gcgtggtcgg cggcggcctg ctcggcgtcg gccgccgcct
      121 cggcgagggc ctcctcggca cgggtggtcg cggcctgccg cgaggccacc agggcggcca
      181 ggtcgtcgat ggtgaggagc gcccggtact cgtccgactg ctccaggccc cgcaggctcc
      241 cggtggcgtc gcgctgctcg accctggccg tctcccgtgc ctgctccagt tcgagcacga
      301 acgcctcggc ggcgacctgt tcccggtcgg cctggcggtg ctggcggcgg gcggcctgcg
      361 cggcccggtc cgcctcggcc agcgccagcg cccgggcacg cgcctcggcg cgcaggtagt
      421 cgcggtacca gggcagcgcc tcgttgacgg cggcgacggc ggcgcgcagc tccttcagcc
      481 gcccccggat ccggtccagg tcctcgaacg cggcggcgac cgtctcgatg acgtcgccgg
      541 cgagcggggt gagcgcctcc gacagcgacg cggacaggcc gtccaggtcg agctgcttgg
      601 ccaggtgcgg gcggcgcagc accagcacga ggttgaccag tttgcgcagg cggtccacgc
      661 ccatcccgaa cagggcccgg tcgaccgccg cctggtactc gtcgatggtg tcgaacacgc
      721 acgcgctgac cagccgccgc cgtccccgcc gcccgggcgc gggcgcctcg acgtcgcctt
      781 ccagctcgtc ggcgggccat tcgtcgtcct cgggttccgg gtccggccgc tcgggacggt
      841 tccccgcggg ttcgtccacc ggatcggtga tctcggcgtc tacgggaggt ggcaggtggt
      901 cgccgagggc ggcgatcagg ccgccgcggg tgagcgggac gccgccgggg accaggtcca
      961 ggtcgacgcc gacgcgccgg ctggtgacga agaaccaggt ctcggcgtcg cggctggtgg
     1021 tcgcccgcgc cccggcgccg atggtcaccc agcgcgggcc ggcgggggtg tcgcggcgca
     1081 gctccagcca gacgtagccg acccgcgagt cgaactcgta gcggcgcggg ccgtcgcccc
     1141 gccgctggca cagcagcagc gacttgaggt tgcgcccgcc gccatgcggg tccatgtagg
     1201 tcgcgccggc gcggccgtcg agcaggaacg ggaacagcag cgccagcgcg ttcgacttcc
     1261 ccgacccgtt cgggccgcgg aagatcaccc gcccgccggc gaagtggagg acggcgtcgt
     1321 cgtactccca gacgttgcgg agccccgccc gggtcggccg gaaccgcccc ggccggcgcc
     1381 gctccggcgg ccgggtgcgc cggccggcgc cgtcgccgtt cgcggtgaac agggtggaca
     1441 tcagcgtcgc ctcctccggc gggccggccg cccgccctcg ccgggccgga agcgggcggc
     1501 gagcgggcgc agcaccacgg ttcccggcgg ctcctccggc ccgggcgcca ccgccagccc
     1561 gagccgctcc agcagcgcca gcgccgccac ccggagggcg tccgggtcgg cctggtaggc
     1621 gacggtgaag cggtcgccgt gctgcctgac catggccgcc caggtcgcct cgaccgcctc
     1681 gggcgggatg cggcggccca cggggagctg cccggcgacc tggccctgcc cggcctcggc
     1741 gtcggccagc tcgtcggccc acagcagcgc gaggttgttg acgatcccct ggccggggaa
     1801 cggctgatcg ttcaggcgcg gctgcccggc ggagtcgatc agggcgaggc cctcggcgcg
     1861 gacctcgacc tcgcagccgg tgagccgtgc caggtcgccg accaggcggg cgcggcgctc
     1921 ggcgaggtgg tcgcgctcgt cgacgggcag ttcgtcatag tagacggcgg gctcgtcgag
     1981 caggcgccgg gagaggcggt ggcgcaggcg cgcgtcggtg gtgtcggcac ccggcgggta
     2041 gcgctcctcg cagaactggg ccagcccccg ctccggctcg atccacgcgg gagtggggcc
     2101 gagcaaccgc cgggccgcgt cgtggtcgac ggtgtagagg gcgtcggcct cctcgtcgtc
     2161 gatgaaggcg atcgtctcgc cgtcgggcgc ggtgcgcagc acgccgagcc gctccaggta
     2221 gccgaccgcg tggacgaacg cgcggcggta ctcgatgcga ccgaagtcaa ccgggaggcg
     2281 ctcgtcgccc gcgcgcagcc gctgcgcccc ctcgaccagg cggcggatgg tggtctggcc
     2341 gccctcgccg tgcagcgccg ccagtaccag gcacagcagc acgtagtcga gccgcccgaa
     2401 cggccggggg gagcgctcgc cgccggcgct ggtcaccatc gccgggcgcc gcggcgacgg
     2461 ctcgacccgc cgctgcacca gccgggcgca gctcggcgcc acctccagcc gccagcgggt
     2521 gagacgctcg aactcgttgc gcagggtggt ctcccagcgg cgcaccttcg gcagcgcctc
     2581 ggggttcggg taggtcgcgg tgaccagcgg gtgcgccagc agggtgcggg ccgcctcctg
     2641 gaagtcctcc agttcgaccg ggtcgagcat ggacgcgtag cgggccgggc tcatcttggg
     2701 accacctcca ccgccaggcc gtcgaagacg aggtcgccgt ccacgcagcg gatcacgcag
     2761 ccagccgggt cgggacgggc gcgcaccgtg accccttcgg cggcggcgag gaccgccgcg
     2821 ccgtccgcgc cgtccgcgcc gggaaggccg agcacgccga gcaggcactc gtcgaccagg
     2881 gagagcagca gctccagctc cgcgcgggag aggatccggc cggccagcgg cgccgaggcc
     2941 aggcgcgtcg ccgcctcggc ccgctcgcgc tcggcggccg ccgcatcggc agcgagcgcc
     3001 tcccgctggg cggagaagtc gaccgcccgc gcggtcttgc ccggggtgct gtgctggccg
     3061 cgctcgcgca gcgacaccgc gatctcgacc ggctcggcgc tccaccagga ggcacgcggg
     3121 ccggtcgcgg tcgggtcggg cggcagcccg ctcacccgcg gggtgcggcc cagcccgaag
     3181 gccgcgtcga acagggcgtg cgcctcggcc gcgccgtcgc agcggtggaa ccagcgcgcc
     3241 agcgcgagca gctccgaggt gcggttgacc gggcggctct cggcctcgga caggcgccgg
     3301 atctgcccga ggacgtcccg caccgcctgg gtggtggcgc tgaacagctc gtccaggccg
     3361 gacggacgcc cgtcggaggc gctgagccag cgctccagcc cggtccagcg gctctgccag
     3421 ctcgcccgca ggtcgcggcc gaggcccagc gccgccgccg cgccgtcgtc gccggccgcc
     3481 gccagctcga ccagccggtc caccccctcg tccgtgatct ccgcggcggt cgcggccatg
     3541 cggcggcggt agcggtagag gtcgtccgag aaggtcgaga ggtaggtgag cagcagctcc
     3601 ttgcagtcga ggaacaccgc ggcgtccagc tccgacccgc gggtcagctc cgccagcttg
     3661 gtggagaagt cctgcgccga gcgggtcagg tcgcgcaggt cggtgtcgag cgagcgcagc
     3721 agcccgtaga cgccgccggc gtcgccggcc cggaccgccg ccagcagcag ctccaggttg
     3781 cgccgcagcc cctcgaacag ccgtgcccgc aggccccccg cggcctcgac cgccgcgagc
     3841 accgcttgcg ccgcctcgaa ggccgccacc ccgaccgccg tcaggtacca ctgcgcccgc
     3901 cgccggtaca tgtcctcttt ccgggtcacg tgccgcgtgt ccggggtcca gtcgacgcac
     3961 cccatcccct tgagagcgtc cagcacctcg atcagcttgt cgaagtccag ctccagcgcg
     4021 ctggccggca gcatcgccag cagctcctcg gtccgcagct gcggccggta ggccgcccgc
     4081 gccgccacca tcgccgacag gatcgccagg taccgctccg cccgctcggt ggtccccacc
     4141 agcagtgccc gcagccggtc gcgctccccg gcctccagca gccgctcgtg aaacgcccgc
     4201 gccccggccg ggggcacctc gcccggcgcc gcccgggtct gcccggccac cgcgacggcg
     4261 gccggttccg gggtgggagc ctcgggcgcg tgtccgttcc cgctctggcc gtccatggcc
     4321 acagcgggca gctcggtggt aacaagggga ttgttcgcca taccgcgaac cctatctgga
     4381 gcctgcgaca gcgcccggtt caaggcgcac agtatgtccg ggtcgggacg atccgtgcgc
     4441 tgctctccgt agagttgacg atacggccgt ccccacccgt ggaacctttg gtccacagtt
     4501 cggcgtcaac cgcgacggcg gcgaccgcga tctcctgcct ggccggtgtc gcaggaactc
     4561 gaccctgatg aggtgtcggt gaggaccgcc atagttgccg atctcgcgtc gacgacgccc
     4621 gcacgctgga accgctcagc gcgggctggc ggcgacgaag cggaagcggt cgccgtcggc
     4681 gtccacgtcc accgccgccg tggcgaagcc cgccgcgcgc aggcggcccg gcagctcctc
     4741 gggaagcgcc ggcacgaaca ggtcgtcaat atgcgcttcg cggcggctgg gcgtgtccat
     4801 gccgtcggtg ccgaccagga ggccgcctgg tcgcagcacc cggcagagct cgccgagcat
     4861 ccggtcctgc agtgccacgc tggggacgtg gtgcagcatg gtgaagctgg ccacggtgga
     4921 gaacgcccgg ctgcggaagg ggagccgggt ggcgtcggcc cgcaccacct cgacacccgc
     4981 gccggacagg cgctggccca gagacgctgc cagctgctgg tccagctcga ccgccaccag
     5041 ccgggggacg cgcgggcgga gcgcgtcggt gaccacgccc ggtccggccc cgacctcgag
     5101 cacctggtcg ccgagctggc gaccctccag cacccacggc agcacccgct cgacggtctc
     5161 ggcccactcg gcgctggcgc aaaccttgag gtggtactcg ttcaccgcct gagcgtagct
     5221 ccagcgcgtt gccagtgcga ccaggacgca cgatgcggcc ttgcgtgcct cccggttcgg
     5281 gtgccgtcat gatgcggcag cggcctcggg tcttccaggc tcgcactggc agctcccaag
     5341 gaacaaggag cgcacgtgaa cgggattcca gacagccacg cggacatcct tgacaaggca
     5401 ggcttggcat atctcgccac ggctgggccc aagggggagc cgcaggtgtc gccggtctgg
     5461 ttcgggtggg acggccgcta cctccgggtc agtcacacgc cggaccggca gaagtaccgc
     5521 aacgtgcggc gcgacccgcg ggtggcgctg gcgttcaacg accccgacga cctctaccgc
     5581 cacatcgaga tccgcggccg ggtggagcgc atcgacccgg accccgacca cgccttcatc
     5641 aacgccatgg ccaagaagta cacgggcgtc gacgtctacg aggccgcccc gccgagccag
     5701 gagcgggtca tcctggtgat cgccccggag cacgccaata cgatgggctg accagcgtgc
     5761 tattctgacc agtgaccatg gtcagtggtc agaatagaga gggacatggc ggagagcgtg
     5821 atggaagagc gtgttgtgat ggccagtcga ttcaaggagc gctgccttgc cctccttgac
     5881 caggtggcgg agacaaaggt gcccgtcgtg gtcaccaagc acggaaagcc ggtggccaaa
     5941 ctggtcccga tcgacgagcc agtggccaag cccatgatgg gcagcgtgcg gctcctcgtc
     6001 gaggacgacg agtcgtactt ctcgactggg gagagctggg tcgctgaggg gcctcacggc
     6061 gaatgatcct gctcgacacg cacgccctgc tctggtgggc ggcgggtagc gaccggctct
     6121 cgcccggagc agcccgcgcg atcgccaggg ccgacacgat cctcgtcagc cccatctcgt
     6181 gctgggaggt cgcgactctg gtcggcaagg ggcgaatcgc gctcgaccgc gacgtctacg
     6241 tctgggtcct ggatctcttc agtctggacg aggtagagct tgccccgctg actccccaaa
     6301 tggcggtcgc cgccgctctc cttggcacgg ccggattcca tggcgacccc gccgaccggt
     6361 tcctctatgc caccgcactg gagcactcgg tcccgctcgt caccaaagac gagtcgatcc
     6421 ggtcctatgc cgagaaggcc ggcgacctcc gcacgatctg gtagcggtaa ggcgtgtgat
     6481 tggcttgttc gacgccaatc gtgtaaaacc ctcgcagcac cgcgaagcag agggggcttg
     6541 ccatgccgga tccgtggttc atcgggccga aactgcggta ctggcgcaag cggcgcggga
     6601 tctccgcgca gaccctcggc ggcctggtcg ggcgctccca cagctgggtg accatggccg
     6661 agcagggccg ccgcgccccc taccggctcg acgacctggt cgacctcgcg gcggcgctcc
     6721 gggtcgacct cggcaccttc gtgtgcaccc ccgtccccgg ggtcccggac ccggcccacc
     6781 agcaggtcct cggccggctc caggcggcgt tcggcgggcg tgacccgcgc tcaggcttag
     6841 ccgatcttgc gcaaacgatc ggtttgtcag accctggagc cgacctgatg ctgatcgtcg
     6901 agccagcggg aggcctcaaa atcgtgaacc gccgcgaggc cctccagacc ggcggcctga
     6961 tcggcatcag cctggctctc cccctcggac ccttgacccc gaccagtccg ccgccctcgc
     7021 cgcctcgatc gggaaacgca agatcggcat gtccggcgtc cagagcctcc gaacgatctt
     7081 gggtgagttc cgccgcctgg acgaccagct tgggtcagcc ggactccggc aggcggtgct
     7141 cgtccatctg cgtctggtgc aggcattgcg cggcaccggg agaaccgagg aggccgaatc
     7201 cgcgctcggc tcgatcgagg cggagttcgc ccagttcgcc ggctggctgg cattcgatgc
     7261 ctgtgactac cgcgcggccg agcgctacct cctgatcgcc tacaaggccg ccgacagcga
     7321 aggcgac
//
LOCUS       DASWDC010000362        10456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1585608, whole genome shotgun sequence.
ACCESSION   DASWDC010000362 DASWDC010000000
VERSION     DASWDC010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10456
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585608"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /gene="secF"
                     /locus_tag="VF995_11590"
     CDS             complement(<1..1155)
                     /gene="secF"
                     /locus_tag="VF995_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX9344242.1"
                     /translation="MSLASRLYHGQIRVDFVGHWRRWVLISGIAMLISVIALFFPGLK
                     FGLDFRGGGLFTATPSHPVTQGDVSSALGPVAKIVQITGAQPGKPSRVVVQTEPLDTA
                     QTAVVRANLARVAGVGSNQVDTTVIGSKWGSTVSRAMIKALLAFLIVVTIYVSIRFEF
                     KMAVTALIALIHDLLITAGIYALARFEVTPATVVALLTILGYSLYDTVVVFDKVRENT
                     GELSSMSRITYSDAANRAVNQTVVRSLNTSLTSLLPIGALLFVGAALLGAETIKDLAL
                     ALFVGVATGTYSSIFIATPLVALWKEREPRNQQLRARVARLAAQPQPAAAAAAAGAGG
                     GGGGRAVTSGGSGKAARRPQRQAASAAAATASDGQADGQRNGLGPEADQAQ"
     gene            complement(1216..2667)
                     /gene="secD"
                     /locus_tag="VF995_11595"
     CDS             complement(1216..2667)
                     /gene="secD"
                     /locus_tag="VF995_11595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX9344243.1"
                     /translation="MPGNRRLIMPMIALLTIVAAIWGTILFQHITPRLGLDLRGGSSV
                     TFAPSNPNGAPVTKEQIDRTIDIIRNRVNSSGVAEAEVSSEGNNIVVAIPDVKDPDVV
                     IQRVQTTAQLQMRPVLAQAAAGSATYKQAPFNTVDCSKPESYTAPDNPDKTVVLCTNI
                     SGKLQVDPSGTKLQLGKVALQGADVSSAQAQLATSGNNITTGQWVTNLNFSGAGQKKF
                     ATLTGNAACNTQGTPQTEIAIILDHVVINSPPVATDVQCHVGITQGGQITGQSESEAK
                     FLAPLISSGALPLTLTQQDRNTVSATLGSDSLRAGLIAGAIGLAATFLYVMLYYRGLG
                     LVIWLGLTIAALLNVGVVLLMGKYQGFTLTLAGIAGLIVAIGISADSYVVFFERLKDE
                     VRDGKTLRTSVDRGWQRSFHTLVSANTVSFAAALILYLLAICPVRGFAFTLGLATLVD
                     FFAVWMVGRPVVTLLTRTRLFQEGRFVGIRAAV"
     gene            complement(2726..3433)
                     /locus_tag="VF995_11600"
     CDS             complement(2726..3433)
                     /locus_tag="VF995_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9344244.1"
                     /translation="MSLAERRPPAPVAEPRLLDRVASNRVVQTYLALADQFTGQLGYT
                     EHGPRHAGLVGAIAYNILDRLDYDHHAAELAAVAGYLHDIGNVVSREHHPQTGALIAQ
                     PLLAEAGLPPEDAGLVMGAIGNHEETMGLPVSPIAAAVIIADKSDVHRSRVRKDAHAQ
                     IQFDIHDRVNFSATHSFVRVDPERRTITLELEIDTTVSDILEYFEIFLTRMMMCRRSA
                     EGLGCKFRMTVNGNSLL"
     gene            complement(3437..3802)
                     /gene="yajC"
                     /locus_tag="VF995_11605"
     CDS             complement(3437..3802)
                     /gene="yajC"
                     /locus_tag="VF995_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX9344245.1"
                     /translation="MLAANSGAGAQLILFALLFAVIYFLMIRPQRRRAQQQASLQRSL
                     ELGDEVMTVSGFLGTIRRFDGETVTLELSPGVEVRVVRRAISGKVQPSEPEAEELDEH
                     LGLDGHDHQHDDEAGNPGT"
     gene            complement(4060..5118)
                     /gene="ruvB"
                     /locus_tag="VF995_11610"
     CDS             complement(4060..5118)
                     /gene="ruvB"
                     /locus_tag="VF995_11610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX9344246.1"
                     /translation="MSDLLAPSASPEERASEGSLRPRKLEEFVGQARTREQLAIVLES
                     ARRRGLPPDHLLFSGPPGLGKTSLAAIVAAEMGAGLRITSGPALERGSDLASILTGLE
                     PGDVLFVDEVHRLPRAVEEVLYPAMEDFQIDVVLGKGPGARSLRLDLPRFTLVAATTR
                     TGLITSPLRDRFGFSAKLDFYQPAELQAIVTRSASILGVVIDAAGAAMLARRSRGTPR
                     IANRLLRRVRDYAEVRGDGTIDAATAASALDLFEVDPLGLDKLDLALLRVLCERFAGQ
                     PVGLATVAVSVGEEPDTVEDVAEPYLLQLGFLIRTPRGRLATAAAFAHLDLPVPRDVP
                     GAPASGTLFDQAASEPDS"
     gene            complement(5135..5749)
                     /gene="ruvA"
                     /locus_tag="VF995_11615"
     CDS             complement(5135..5749)
                     /gene="ruvA"
                     /locus_tag="VF995_11615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX9344247.1"
                     /translation="MIASLRGRLVEVLADGAVIDVGGVGYRVFLTPKALAALPRGGEV
                     LVHTVTYVREDALALYGFLSADERRVFEQLLSASGVGPKLALAMLSVHSPDALRRAVS
                     AGDADALTLVSGVGRKGAARLVLELKGKLGGDGGYELPAEGGAGRAAYAEVREALAAL
                     GCAPAEVKAALESLPADAGELSTEELLRLALRNLGPGGTVRGGR"
     gene            complement(5746..6348)
                     /gene="ruvC"
                     /locus_tag="VF995_11620"
     CDS             complement(5746..6348)
                     /gene="ruvC"
                     /locus_tag="VF995_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX9344248.1"
                     /translation="MRVLGIDPGLSRCGWAVLEQAGGPVKALGYGTVVTKGEDVPGRL
                     ATLAARLRGVIAEHRPEQVAIERLFVNANVRTAMTVGQASGVALLLAAEHGLPLSTYT
                     PSQVKQAVTGTGTAPKEQVGYMVRAILRLEAIPRPADTADAIAVALCHLHHAGTAAAF
                     GQRPGTGTGAPGGRGSGALSAATAADWLARAAPTSPGLPR"
     gene            complement(6441..7187)
                     /locus_tag="VF995_11625"
     CDS             complement(6441..7187)
                     /locus_tag="VF995_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9344249.1"
                     /translation="MSGHSKWATIKHQKGAKDARRGKLFAKLIRAIEVAARTGGSNPE
                     GNATLADAIQKAKDNSVPNDTIDRAVKRGAGEVGGQQLELVTYEGYAPGGVAVLIEAL
                     TDNRNRAAAAVRHAFAKAGGSLAESGSVAYLFNRKGQLVVIGDAADEDRVMEIALEAG
                     AEDVQSDNVGFTVTTAPGDLRAVRAAFDNAGIPYQSAEVTMVPSLSVPLDRDGAIKVL
                     RLLDALDDVDDVQDVYANFDIPDEIMAEVS"
     gene            complement(7205..7825)
                     /gene="pdxT"
                     /locus_tag="VF995_11630"
     CDS             complement(7205..7825)
                     /gene="pdxT"
                     /locus_tag="VF995_11630"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX9344250.1"
                     /translation="MAHPPLTIGVLAYQGDVREHLQVLGALRGVGGRGPAVVAAREVR
                     TPADLDQVDGLIVPGGESTVIGKLASRYGLLEPLRQRAAGGFPLFGTCAGMIFMARAV
                     EGPPQHLLGVMDVAVRRNAFGPQVCSFEAEVDVKGIDGGPVSAAFIRAPWITEAGADV
                     DVLAELDGRVVAARQGNLLATAFHPELTGETRLHRWFADLVASRHD"
     gene            complement(7838..8728)
                     /gene="pdxS"
                     /locus_tag="VF995_11635"
     CDS             complement(7838..8728)
                     /gene="pdxS"
                     /locus_tag="VF995_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX9344251.1"
                     /translation="MESTNHATGTARVKRGLAEMLKGGVIMDVVTAEQAKIAEEAGAV
                     AVMALERVPADIRAQGGVARMSDPQLIHEIIEAVSIPVMAKARIGHFVEAQVLQALGV
                     DYVDESEVLSPADEAHHVDKWRFTVPFVCGATNLGEALRRLAEGAAMIRSKGEAGTGN
                     VIEAVRHMRSISNGIRRLAGLREEELFAAAKDLGAPYELVAEVAREGKLPVVLFTAGG
                     IATPADAALMMQLGAEGVFVGSGIFKSGDPARRARAIVEATTFYNDPTIIAKVSRGLG
                     EPMVGISSVPEQERLAGRGW"
     gene            complement(8844..9809)
                     /locus_tag="VF995_11640"
     CDS             complement(8844..9809)
                     /locus_tag="VF995_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9344252.1"
                     /translation="MDSAHAASGLLLAQNRFAAELRRWRELRRLPKKQLASAMGYDPS
                     YVSHIEGCNLHPTEGFARRADAVLRAGGSIWQRWREFAAARVTVGGAEPERPPERPIE
                     PGQLIVERDQASLEHHGGSYQLRMAKRLRNASDSVVTRFLIRIAVDRYPGQADRSNRH
                     YRTNPLTLPELDLRAWCGDGEERGAEPMAWEAKHDRDSFKELWLLFENGQGKFPLYPG
                     EATWIRYSYTVGVDKWGHWFQRAVRLPTLRLSVQLRFPLALQPVVWGTETSLARGELP
                     LPTPVRRRDEGEWACFDWATDQPLPQARYRLEWRFRNPGEVRPER"
     gene            9976..10254
                     /locus_tag="VF995_11645"
     CDS             9976..10254
                     /locus_tag="VF995_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344253.1"
                     /translation="MQRDDEVRNHLEAAAGLLRQAPPDPELLMLVAHDIATLIGVLGR
                     GAARAGSPLPFAAVQSITELRRWALDLRASLGGGPGGPGTDPGTGTER"
     gene            complement(10261..>10456)
                     /locus_tag="VF995_11650"
     CDS             complement(10261..>10456)
                     /locus_tag="VF995_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9344254.1"
                     /translation="TKLRDAPNAAQASNLGLNHLWIVAPGPGPGPGPATTAGSEDSGL
                     ERLFDTHPPLGDRIEALREL"
BASE COUNT         1465 a         3562 c         3879 g         1550 t
ORIGIN      
        1 ctgcgcctgg tctgcttccg gtccgagccc gttgcgctgg ccgtcggcct ggccgtcgct
       61 ggcggtggcc gccgcggctg aggccgcctg ccgctgcggc cgccgggcgg ccttgccgct
      121 gccgccggag gtgaccgcgc gaccgccacc gccaccaccg gcgccggcgg ctgccgctgc
      181 cgctgccggc tgcggctggg cggccaggcg ggcgacccgg gcgcggagct gctggttgcg
      241 gggctcgcgc tccttccaca gggccaccag cggggtggcg atgaagatcg acgagtaggt
      301 cccggtggcg accccgacga acagggcgag cgccaggtcc ttgatggtct cggcgccgag
      361 cagcgcggcg ccgacgaaca gcagcgcgcc gatcggcagc agcgaggtca gcgaggtgtt
      421 cagcgaccgc accaccgtct ggttgacggc gcggttggcc gcgtcggagt aggtgatgcg
      481 cgacatcgag ctcagctcgc cggtgttctc acggaccttg tcgaacacca ccacagtgtc
      541 gtacagcgag tagccgagga tcgtcagcag cgcgaccacc gtcgccgggg tgacctcgaa
      601 acgggcgagc gcgtagattc ccgcggtgat cagcaggtcg tggatcagcg cgatcagcgc
      661 cgtgaccgcc atcttgaact cgaagcggat cgacacgtag atggtgacca cgatcaggaa
      721 ggcgagcagc gccttgatca tcgcgcgtga cactgtgctg ccccatttgg agccgatcac
      781 ggtggtgtcg acctggttcg acccgacccc cgcgaccctc gccagattcg cgcggacgac
      841 ggcggtctgg gcggtgtcga gcggctcggt ctgcaccacg acccgcgacg gcttgccggg
      901 ctgggcaccg gtgatctgga cgatcttggc gaccggcccg agcgccgacg agacgtcgcc
      961 ctgggtgacc gggtggctcg gcgtggccgt gaacaggccg ccgccgcgga agtcgaggcc
     1021 gaatttcagg ccggggaaga acagcgcgat caccgagatc agcatcgcga tgcccgagat
     1081 caggacccag cggcgccagt ggccgacgaa gtcgaccctg atctgaccgt ggtagaggcg
     1141 gctggctagc gacacgaggg cgtgctctcc ttggacggtt ccaactctaa gccttgctcg
     1201 aagccttggg cgccactaca cggcagcgcg gattccgaca aagcgcccct cctggaacag
     1261 gcgggttctg gtcagcaggg tcaccaccgg ccggccgacc atccacacgg cgaagaagtc
     1321 gaccagggtg gcgagcccca gggtgaaggc gaacccgcgt accgggcaga tggcgagcag
     1381 gtacaggatc agcgcggccg cgaacgacac ggtgttggcc gagaccagcg tatggaagga
     1441 gcgctgccag ccgcggtcca ccgaggtccg cagcgtcttg ccgtcgcgga cctcgtcttt
     1501 cagtcgttcg aagaacacca cgtaggagtc ggccgagatg ccgatggcga cgatcagccc
     1561 ggcgatccct gccagggtca gggtgaaccc ctggtacttg cccatcagca ggaccacgcc
     1621 gacgttcagc agcgccgcga tggtgagccc gagccagatg accagcccga ggccgcggta
     1681 gtagagcatc acgtacagga aggtggcggc cagcccgatc gcgccggcga tcagcccggc
     1741 gcgtagcgag tcgctgccga gcgtggcgct gacggtgttg cggtcctgct gggtgagggt
     1801 cagcggcagc gcgccgctgg agatcagcgg cgccaggaac ttcgcctccg actcgctctg
     1861 cccggtgatc tggccgccct gggtgatgcc gacgtggcac tgcacgtcgg tagcgaccgg
     1921 cgggctgttg atcaccacgt ggtcgaggat gatcgcgatc tcggtctggg gggtgccctg
     1981 ggtgttgcag gccgcattgc cggtgagcgt cgcgaacttc ttctggccgg cgccggagaa
     2041 gttcaggttg gtcacccact ggccggtggt gatgttgttg ccgctggtcg ccagctgcgc
     2101 ctgcgcggag ctgacgtcgg cgccctggag cgcgaccttc ccaagctgca gcttggtgcc
     2161 gctggggtcg acctggagct tgccgctgat gttggtgcac agcaccaccg ttttatccgg
     2221 attatccgga gcggtgtagc tctcgggctt gctgcagtcg acggtgttga agggtgcctg
     2281 cttgtaggtg gcgctgccgg cggcggcctg ggccaggacc ggccgcatct ggagctgggc
     2341 ggtggtctgg accctctgga tgaccacgtc ggggtctttg acgtccggga tggcgaccac
     2401 gatgttgttg ccttcgctgg acacctcggc ctcggccaca ccgctcgagt tcacccggtt
     2461 gcggatgatg tcgatggtgc ggtcgatctg ctccttggtt accggggccc cgttgggatt
     2521 gctgggcgcg aacgtgacgc tgctgccgcc gcgcaggtcc agcccgagcc gtggcgtgat
     2581 gtgctggaag aggatcgttc cccagatcgc ggcgacgatc gtcagcaggg cgatcatcgg
     2641 catgatcagc cggcggtttc ctggcactgc gcttcgcgct ccttatgtct cttgctcctg
     2701 gccgctgcct gtgaagacgc ttcggctaca gcagcgagtt gccattcacg gtcatgcgga
     2761 acttgcagcc gaggccctcg gcggacctgc ggcacatcat catcctggtg aggaagatct
     2821 cgaagtactc gaggatatcg gacacggtgg tatcgatctc cagctcgagc gtgatggtgc
     2881 ggcgttcggg gtcgacccgt acgaacgagt gggtggccga gaagttcacc cggtcgtgga
     2941 tgtcgaactg gatctgcgcg tgggcgtcct tgcggacccg ggagcggtgc acgtcggact
     3001 tgtcggcgat gatcaccgcc gccgcgatcg gcgagaccgg cagccccatg gtctcctcgt
     3061 ggttgccgat cgcgcccatc accagcccgg cgtcctccgg agggaggccc gcctcggcca
     3121 gcagcggctg ggcgatcagc gcgccggtct gcgggtggtg ctcgcggctc accacgttgc
     3181 cgatgtcgtg caggtacccg gcgacggcgg ccagctcggc ggcgtggtgg tcgtagtcga
     3241 gccggtcgag gatgttgtag gcgatcgcgc cgaccaggcc ggcgtgccgc gggccgtgct
     3301 cggtgtagcc gagctggcca gtgaactggt cggcgagggc gaggtaggtc tggaccaccc
     3361 ggttgctcgc cacccggtcg aggaggcgag gttcggcgac gggggcaggg ggccgccgct
     3421 cagccaagct catcgttcat gttcccgggt tccctgcctc gtcgtcgtgc tggtggtcat
     3481 ggccgtccag gcccaggtgc tcgtccagct cctcggcctc aggctcggac ggctgcacct
     3541 tgccgctgat cgcgcggcgc acgacccgca cctcgacacc gggcgacagc tccagggtga
     3601 ccgtctcgcc gtcgaagcgg cggatggtcc cgaggaaccc cgagacggtc atgacctcgt
     3661 cgccgagctc cagcgaccgc tgcagcgacg cctgctgctg ggcgcggcgg cgctgcggcc
     3721 ggatcatcag aaagtagatc accgcgaaca gcagcgcgaa caggatcagc tgtgcaccag
     3781 caccgctgtt cgcggcgagc acgattccca gcgcaccagc accgttcccc atagcgtgaa
     3841 gacctccgaa gcgcccaggt ggatcccaag gcctgcatcc cacctgaagc tggtgggggc
     3901 acgtcaacgc tgcggcgtcc ggccacggcg caggcgccat cctagggatg gaacccagtg
     3961 cggtcaactg ccgccgatgc cccgaacctg tgggctgagc tgcgctttcc ggaatctatc
     4021 ctagcgcacc ggggaccgtt ggggaggatc cggacagccc tatgaatcag gttcgctggc
     4081 cgcctggtcg aacagcgtcc ccgaggcggg cgcaccgggc acgtcgcggg ggacggggag
     4141 gtcgaggtgg gcgaaggcgg cggcggtggc gaggcggccg cggggggtgc ggatcaggaa
     4201 gccgagctgc agcaggtagg gttcggcgac gtcctcgacg gtgtcgggct cctcccccac
     4261 cgagacggcg acggtggcga ggccgaccgg ctggccggcg aagcgttcgc acagcacccg
     4321 cagcagcgcc aggtcgagct tgtcgagacc gagcgggtcg acctcgaaca ggtcgagggc
     4381 ggacgcggcg gtggccgcgt cgatcgtgcc gtcgccgcgg acctcggcgt agtcgcggac
     4441 gcggcgcagc agccggttgg cgatccgggg cgtgccccgg gagcgccgcg cgagcatcgc
     4501 ggcgcccgcc gcgtcgatca ccaccccgag gatcgaggcc gagcgggtca cgatcgcctg
     4561 cagctcggcg ggctggtaga agtcgagctt ggcggagaag ccgaagcggt cgcgcagcgg
     4621 cgaggtgatc agcccggtgc gggtggtcgc cgcgaccagg gtgaagcggg gcaggtcgag
     4681 gcggaggctg cgggcgccgg ggcccttgcc gaggacgacg tcgatctgga agtcctccat
     4741 cgccgggtag aggacctctt cgaccgcgcg gggcaggcgg tggacctcgt cgacgaacag
     4801 cacgtcgccg ggttccaggc cggtgaggat cgaggccagg tcactgccgc gttccagcgc
     4861 cggcccggag gtgatccgca ggccggcgcc catctcggcg gcgacgatcg cggccaggct
     4921 ggtcttgccg aggccgggcg gtccggagaa cagcaggtga tcgggcggca ggccgcgccg
     4981 ccgggccgac tccagcacga tcgccagctg ctcgcgggtg cgtgcctgcc cgacgaactc
     5041 ctccagcttg cggggccgca ggctcccctc gctggcccgc tcctccggcg aggcgctcgg
     5101 cgccaagagg tcgctcattc gcgcgcgccg tggctcatct gccgcccctg acggtgccgc
     5161 ccgggccgag gttgcggagc gcgaggcgca gcagctcctc ggtggacagc tccccggcgt
     5221 cggcgggcag cgactccagc gcggccttga cctcggcggg ggcgcagccg agcgcggcga
     5281 gcgcctcacg gacctcggcg taggctgccc ggccggctcc cccctcggcg ggcagctcat
     5341 agccgccgtc gccgccgagc ttgcccttca gctccagcac cagccgggcc gcgcccttgc
     5401 ggccgacgcc ggaaaccagg gtgagcgcgt cggcgtcccc ggcgctgacc gcgcgccgca
     5461 gcgcgtcggg ggaatgcacc gacagcatcg ccagcgcgag cttcggcccg accccgctgg
     5521 ccgacagcag ctgctcgaag acgcgccgct cgtcggccga caggaacccg tagagcgcca
     5581 gggcgtcctc ccgcacgtag gtgacggtgt ggaccagcac ctccccgccc cggggcagcg
     5641 ccgccagcgc ctttggcgtg aggaaaaccc ggtagccgac cccgccgacg tcgatcaccg
     5701 ccccgtcggc cagcacctcg accagccgcc cccgcaggct cgcgatcacc gcgggagccc
     5761 cggcgaggtc ggcgccgccc gggcgagcca gtcggccgcg gtggccgcgc tgagcgcgcc
     5821 gctcccccgc ccacccggcg ccccggtccc cgtcccgggc cgctggccaa acgcggcggc
     5881 ggtgccagcg tggtgcaggt ggcacagcgc caccgcgatc gcgtcggcgg tgtcggccgg
     5941 cctcgggatc gcctccagcc gcaggatcgc ccgcaccatg tacccgacct gctccttggg
     6001 cgcggtcccg gtgccggtga cggcctgctt gacctgcgac ggggtgtagg tcgacagcgg
     6061 cagcccgtgc tcggcggcga gcagcagcgc cacccccgac gcctgcccga cggtcatggc
     6121 ggtgcgcacg ttggcgttca cgaacagccg ctcgatcgcg acctgttccg gccggtgctc
     6181 ggcgatcact ccccgcagcc gcgccgccag cgtcgccagc cgccccggca cgtcctcgcc
     6241 cttggtgaca accgtcccat agccgagcgc cttgacgggc cccccggcct gttcgagcac
     6301 cgcccacccg caacggctca accctgggtc gatccccagc acccgcacgc ggtcctccac
     6361 ccgaatcgat ccggcctgaa ctggtgtttg gcgagcctat cccctccgac cggcgggctc
     6421 aaccacttcg cggtggttgg tcagctgacc tcggccatga tctcgtcggg gatgtcgaag
     6481 ttggcgtaga cgtcttggac gtcgtcgacg tcgtcgaggg cgtcgagcag gcgcagaacc
     6541 ttgatggcgc cgtcgcggtc gagtgggacc gacaggcttg ggaccatggt gacctcggcg
     6601 gactggtagg ggatgccggc gttgtcgaag gcggcgcgga cggcgcggag gtcgccgggg
     6661 gcggtggtga cggtgaagcc gacgttgtcg ctctggacgt cctcggcacc ggcctccagg
     6721 gcgatctcca tgacgcggtc ctcgtcggcg gcgtcgccga tgacgaccag ctggcctttc
     6781 cggttgaaca ggtaggcgac gctgccggac tcggcgaggc tgccgccggc tttggcgaag
     6841 gcgtggcgga cggcggcggc ggcgcggttg cggttgtcgg tcagcgcctc gatcaggacc
     6901 gccaccccgc cgggggcgta gccctcgtag gtgaccagct ccagctgctg gccgccgacc
     6961 tcgccggcgc cgcgcttgac ggcgcgatcg atggtgtcgt tcggcaccga gttgtccttg
     7021 gccttctgga tggcgtcggc cagggtcgcg ttgccctccg ggttggaccc gccggtgcgg
     7081 gcggcgacct cgatggcgcg gatcagcttg gcgaacagct tgccgcgccg cgcgtccttg
     7141 gcgcccttct gatgcttgat ggtggcccac ttcgagtggc ctgacattgc agctcccttg
     7201 ctcgttagtc gtggcgcgag gcgaccaggt cggcgaacca gcggtgcagg cgggtctctc
     7261 cggtcagctc gggatgaaag gcggtggcca acaggttccc ctggcgggcg gcgaccacgc
     7321 ggccgtccag ctccgccagc acgtccacat cggcgcccgc ctcggtgatc cagggggcgc
     7381 ggatgaaggc ggcggaaacc gggccgccgt cgatgccctt gacgtcgacc tcggcctcga
     7441 aggagcacac ctgcgggccg aaggcgttgc ggcgcacggc cacgtccatc acgccgagga
     7501 gatgctgggg cgggccctcg accgctctgg ccatgaagat catgccggcg caggtaccga
     7561 acagcgggaa gccgccggcg gcgcgctggc gcagcggctc cagcaggccg tagcggctgg
     7621 ccagcttgcc gatcacggtc gactcgccgc cgggcacgat caggccgtcg acctgatcga
     7681 ggtcagccgg ggtgcggacc tcgcgggcag ccaccaccgc gggaccgcgc cccccgaccc
     7741 cccggagcgc gccgaggacc tgcaggtgct cgcggacgtc gccctggtag gccaggacgc
     7801 caatggtgag cggggggtgc gccatcggcc gcatcgccta ccagccgcgg ccggccaggc
     7861 gctcctgctc ggggaccgac gagatgccga ccatcggctc gccgaggccg cgggagacct
     7921 tggcgatgat ggtggggtcg ttgtagaagg tggtcgcctc gacgatggcg cgggcgcggc
     7981 gagccgggtc gcccgacttg aagatccccg agccgacaaa aacgccctcg gcgccgagct
     8041 gcatcatcag cgccgcgtcg gcgggggtgg cgatgccgcc ggcggtgaac agcaccaccg
     8101 ggagcttgcc ttcgcgggca acctcggcga ccagctcgta gggggcgccg aggtccttgg
     8161 cggcggcgaa cagctcctcc tcgcggagtc cggccagccg gcggatcccg ttggagatcg
     8221 agcgcatgtg gcggaccgcc tcgatcacgt tgccggtgcc ggcctcgccc ttggagcgga
     8281 tcatggcggc gccttcggcg aggcggcgca gggcctcgcc gaggttggtg gcaccgcaga
     8341 cgaacgggac ggtgaaccgc cacttgtcga cgtggtgggc ctcgtcggcg gggctgagga
     8401 cctcggactc gtccacgtag tcgaccccga gggcctgcag gacctgggcc tcgacgaagt
     8461 ggccgatgcg ggccttggcc atcacgggga tggagaccgc ctcgatgatc tcgtggatga
     8521 gctgggggtc gctcatccgg gccacgccgc cctgggcgcg gatgtcggcg ggcacccgtt
     8581 ccaaggccat gaccgcgacc gccccagcct cctcggcgat cttggcctgc tcggcggtga
     8641 cgacgtccat gatgacgccg cctttgagca tctcggccag gccgcgcttg accctggcgg
     8701 tgccggtggc gtggttggtt gactccattg cgctggctcc tcgtcctgct ggcccggccc
     8761 cgtaccaccg ccgacagtgc gatgcgtttt ccctgctcag aggcctattc gagagagagg
     8821 ctacaccagg gcacagggcg gcttcaacgc tccggccgga cctcgccggg gttgcggaac
     8881 ctccattcga gccggtagcg ggcctgcggc aggggctggt cggtggccca gtcgaagcag
     8941 gcccactcgc cctcgtcgcg gcgccggacc ggggtcggca gcggcagctc gccgcgggcg
     9001 agggaggtct cggtgcccca gacgaccggc tggagggcca gcgggaagcg cagctggacg
     9061 ctgaggcgca gggtcggcag gcgcaccgcg cgctggaacc agtggcccca tttgtccacg
     9121 ccgacggtgt agctgtagcg gatccaggtc gcctcgcccg ggtagagggg gaacttgccc
     9181 tggccattct cgaacagcag ccagagctcc ttgaaggagt cccggtcgtg cttggcctcc
     9241 caggccatcg gctccgcccc tctctcctct ccgtcgccgc accaggcgcg caggtccagt
     9301 tccggcaggg tgagggggtt ggtgcggtag tggcggttgg agcggtcggc ctggccgggg
     9361 tagcggtcga cggcgatgcg gatgaggaag cgggtgacca ccgagtcgct ggcgttgcgc
     9421 agccgtttgg ccatgcgcag ctggtagctg ccgccgtggt gctccaggga ggcctggtcc
     9481 cgctcgacga tcagctggcc gggctcgatg ggacgctcag gggggcgctc gggctcggcg
     9541 ccgccgacgg tgacccgggc cgcggcgaac tcgcgccagc gctgccagat cgagccgcca
     9601 gcccggagca cggcatcggc tcggcgggcg aacccttcgg tggggtggag gttgcagccc
     9661 tcgatgtggc tcacgtagga ggggtcgtag cccatcgcgc tcgcgagctg cttcttgggc
     9721 aggcggcgca gctcgcgcca acgccgtagc tcggccgcga agcggttctg cgcaaggagc
     9781 aggccggaag cagcatgagc cgaatccata gcggccgcgg ggccccccga tgcatcaagc
     9841 ggttcgggga gctgcggctg aggacctgag tgagccatga gtgagccgtc ctcactctcg
     9901 actaagacaa acacggtcag tttcgcttat cggcagtact ggacgctaga gggctagtca
     9961 aaaagagcta gtacagtgca gcgagacgac gaggtacgaa accatctgga ggccgccgcc
    10021 gggctgctgc gccaggcacc gcccgatccg gaactgctga tgctggtcgc ccatgacatc
    10081 gccaccctca tcggcgtgct cgggcggggc gcggcgcggg cggggtcgcc gctgccgttc
    10141 gccgccgtcc agtccatcac ggagctgcgg cgctgggcgc tcgacctgcg cgccagcctt
    10201 ggtggcgggc ccggcggtcc cggtaccgac cccgggaccg gcacggagcg atgacggcgg
    10261 ctacagctcc cgcagggcct cgatgcggtc gccgagcggc gggtgggtgt cgaacagccg
    10321 ctccaggccc gaatcctccg atccggcggt ggtcgcgggt ccgggtccgg gtccgggtcc
    10381 gggggcgacg atccagaggt ggttcaggcc gaggttgctg gcctgggcgg cgttcggggc
    10441 gtcgcgcagc ttggtc
//
LOCUS       DASWDC010000363         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_497468, whole genome shotgun sequence.
ACCESSION   DASWDC010000363 DASWDC010000000
VERSION     DASWDC010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497468"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VF995_11655"
     CDS             <1..546
                     /locus_tag="VF995_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX9344255.1"
                     /translation="DGARIWNASVASGVPLERYGALFDSLSVCFSKGLAAPVGSAVVG
                     DRDFVERAREVRQLLGGSMRQSGVLAAAALVAVEQMLDRLADDHANARHLAERVAAAV
                     PGGIEAPVETNIVLLAAPAFGTDGPGLVKRLADRGVLAADTAPDQVRLVFHYEISADA
                     TDQAASAVIAAADGTAETAAG"
     gene            543..1544
                     /locus_tag="VF995_11660"
     CDS             543..1544
                     /locus_tag="VF995_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX9344256.1"
                     /translation="MTFGLAEVEAAAARIAGRVHRTPVFHSRLLDRELGCQVLLKGEH
                     LQRIGAFKARGAFNALLALDPAVRARGVITVSSGNHAQAVALAAAETGSHAVVVMPAD
                     ASPAKLAATRGYGAEVICDGVTPANREQFVHRLAAERGLRLVHPFDDFDVMAGQGTAA
                     LELLDEVGDLDLVLAPVGGGGLISGTAAAVKGRSPSTRVVGVEPAGAADARDSLAAGR
                     LVALPEPPQTIADGVRTLRLGERDFAVIRRLVDEIVTVSDDQLRAALALAWSRTKQLI
                     EPTAALPLAALLTGAVGGTAGQRVGVILSGGNLDTTALAATPALPGSNEAAKEEGGP"
     gene            1544..>2601
                     /locus_tag="VF995_11665"
     CDS             1544..>2601
                     /locus_tag="VF995_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9344257.1"
                     /translation="MTVPASASAPTVAGIDVEAIRPQFPGLARTLPDGRPAVFFDGPG
                     GSQPHASAIVAVARHLESSMANLDGAFATARETTALVERARVAGADLLGSAPEGIAFG
                     PNMTTLNFLLAHALARTLAAGDEIVVTALDHDANVAPWLLVARDHGLVVRTAPLRVTD
                     GTLDHDALEGLLGPRTQVVAFPLASNALGTIPDIPRIAAAAHRVGALAWCDAVHSAPH
                     RRIDAAALGVDVVLCSAYKFFGPHVGIAAIRRDLAETLPADQVRPAAESPPGHRFETG
                     TQSHEGLAGMVAAVDYLAGLGTGADPAPGAGRRARLDDAFARIQRHEAALTLRFLDGM
                     ADLLGSAPEGIAFGPNMTT"
BASE COUNT          307 a         1052 c          913 g          329 t
ORIGIN      
        1 gacggcgcgc ggatctggaa cgcctcggtc gccagcggcg tgccgctgga gcgctacggc
       61 gcgctgttcg acagcctgtc ggtgtgcttc tccaagggcc tggccgcgcc agtcggctcc
      121 gccgtggtcg gcgaccgcga tttcgtggag cgcgcccggg aggtccgcca gctcctcggc
      181 ggcagcatgc gccagtcggg cgtgctcgcc gcagccgcgc tggtcgccgt cgagcagatg
      241 ctcgaccgcc tcgccgacga tcacgccaac gcccgccacc tggccgagcg ggtcgccgcc
      301 gccgtgcccg gcgggatcga ggcgccggtg gagaccaaca tcgtgctgct cgccgcgccc
      361 gccttcggca ctgacgggcc cggcctggtc aagcgcctgg ccgaccgggg cgtcctggcc
      421 gccgacaccg cgcccgacca ggtccgcctg gtgttccact acgagatcag cgccgacgcc
      481 accgaccagg ccgccagcgc ggtgatcgcc gccgccgacg gcaccgccga gaccgcggcg
      541 ggatgacctt cggcctcgcc gaggtggagg cggcggccgc acggatcgcc gggcgggtcc
      601 accgcacccc cgtgttccac tcgcggctgc tggaccggga gctgggctgc caggttctcc
      661 tgaagggcga gcatctgcag cggatcggcg cgttcaaggc gcgcggcgcg ttcaacgcgc
      721 tgctggcgct ggacccggcc gtccgggcgc ggggggtgat cacggtcagc tccggcaacc
      781 atgcccaggc ggtcgccctg gccgccgccg agaccggcag ccacgccgtg gtggtcatgc
      841 ccgccgacgc cagcccggcc aagctcgccg ccacccgcgg ctacggcgcc gaggtgatct
      901 gcgacggggt cacccccgcc aatcgcgaac agttcgtcca ccgcctcgcc gccgagcgcg
      961 gactgcgtct ggtgcacccc ttcgacgact tcgacgtcat ggccgggcag ggcaccgccg
     1021 ccctggagct gctcgacgag gtcggcgacc tcgacctggt gctcgccccg gtgggtggcg
     1081 gcggcctgat cagcggcacc gccgcggcgg tcaaaggccg ctcgccctcc acccgggtgg
     1141 tcggggtcga gccggccggc gccgccgacg cccgtgactc gctcgccgcc gggcgcctgg
     1201 tcgccctgcc cgagccgccc cagacgatcg ccgacggggt ccgcaccctg cgcctcggcg
     1261 agcgggactt cgcggtgatc cgccggctgg tcgacgagat cgtcaccgtc agcgacgatc
     1321 agctccgcgc cgccctggcg ctggcctggt cgcggaccaa gcagctgatc gagccgaccg
     1381 ctgccctgcc gctcgccgcg ctgctcaccg gcgctgttgg cggtaccgcc ggccagcggg
     1441 tcggggtgat cctctccggc ggtaacctcg atacgaccgc gctcgccgcg acgccggccc
     1501 tgccgggttc caacgaagca gccaaggaag aaggtggtcc ctgatgaccg tcccagcctc
     1561 agcctcagcg ccgaccgtgg cggggatcga cgtcgaggcg atccgcccgc agttccccgg
     1621 gctggcgcgc acgctccccg acggacgccc ggcggtcttc ttcgacgggc cgggcgggtc
     1681 gcagccgcac gcctcggcga tcgtcgcggt cgccaggcac ctggagtcgt ccatggccaa
     1741 cctcgacggc gccttcgcta ccgccaggga gaccaccgcg ctggtggagc gggcccgcgt
     1801 cgccggggcc gacctgctcg ggtccgcccc ggaggggatc gccttcgggc ccaacatgac
     1861 caccctgaac ttcctgctcg cccacgcgct cgcgcggacc ctggccgccg gggacgagat
     1921 cgtggtcacc gcgctcgacc acgacgccaa cgtggcgccg tggctgctgg tcgcccgcga
     1981 ccacggcctg gtggtccgca ccgcgccgct gcgggtcacc gacggcaccc tcgaccacga
     2041 cgcgctggag ggtctgctcg gcccgcgcac ccaggtggtg gcgttcccgc tggcctccaa
     2101 tgcgctcggg accatccccg acatcccccg gatcgccgcc gccgcccacc gggtcggggc
     2161 gctggcctgg tgcgacgcgg tccactccgc gccgcaccgc cgcatcgacg ccgccgcgct
     2221 cggcgtcgac gtggtgctct gctcggccta caagttcttc gggccgcacg tcggcatcgc
     2281 cgcgatccgc cgcgacctcg ccgagacgct ccccgccgac caggtccgcc ccgccgccga
     2341 gtccccgccc gggcaccgct tcgagaccgg cacccagtcc cacgagggcc tcgccgggat
     2401 ggtcgcggcg gtcgactacc tcgccggcct cggcaccggc gccgacccgg ccccaggagc
     2461 aggccggcgg gcacggctcg acgacgcctt cgcgcgcatc cagcgacacg aggccgcgct
     2521 caccctgcgc ttcctcgatg gtatggccga cctgctcggg tccgccccgg aggggatcgc
     2581 cttcgggccc aacatgacca c
//
LOCUS       DASWDC010000364         5552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U2725
            k141_1163273, whole genome shotgun sequence.
ACCESSION   DASWDC010000364 DASWDC010000000
VERSION     DASWDC010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960375
            Sequence Read Archive: SRR10360621
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,339
            CDSs (total)                      :: 2,314
            Genes (coding)                    :: 2,281
            CDSs (with protein)               :: 2,281
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 29 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163273"
                     /isolate="SMAG_U2725"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VF995_11670"
     CDS             complement(<1..1102)
                     /locus_tag="VF995_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9344258.1"
                     /translation="MELRTVDQAEVSGRRVLVRDDFNVPLDDEGHVTDDLRIRAAIPT
                     LRSLLTRGASVVCCSHLGRPKGKRVEKYSLAPVVSALAEQLGRDVNFVDDVAGQAAHD
                     AAEALRPGEVLLLQNLRFDPGEEANDPTFVDQLASLAEVYVDDAFGAAHRAHASTVGV
                     AERLPAYAGFLLAGEVKELSRLLEGPASPFVAVLGGSKVSDKLAILDNLLGRVDALVV
                     GGGMCFTFLAANGDGVGDSLFEPDQVDAVRDLLARAGREGRRVLLPTDVVVADAFAAD
                     ADHKVVPAGGIEPGWRGLDIGPETAASFAAAVRDAKTVFWNGPMGVFEWPPFAAGTRA
                     VAEAVASSDAYTVVGGGDSAAALAQLGLADRVD"
     gene            complement(1136..2161)
                     /gene="gap"
                     /locus_tag="VF995_11675"
     CDS             complement(1136..2161)
                     /gene="gap"
                     /locus_tag="VF995_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX9344259.1"
                     /translation="MTIRVGINGFGRIGRIFFRAAREAASRSKHDIDVEVVAVNDLTD
                     NHTLAHLLKYDSTFGKLNAEVSYDDTSITVDGQQIKASAERDPAQLAWGDLGVDVVVE
                     STGFFTERDKAALHLKAGAKKVIISAPAKGADATIVMGVNDATYDPATHDVISNASCT
                     TNSVIPLAKVLLDQFGIEKGFMTTVHAFTNDQSTLDFQHKDLRRARTASANVIPTSTG
                     AARAAHLVIPELKGKLDGISLRVPVVDGSLTDLAVILGREVTVEEINAAFKRAAEGDL
                     QGVLRYNTDLIVSSDIVGDPASCIFDSTLTQANGNLAKVFGWYDNEWGYSNRLVDLCQ
                     LVGGSLR"
     gene            complement(2308..3270)
                     /gene="whiA"
                     /locus_tag="VF995_11680"
     CDS             complement(2308..3270)
                     /gene="whiA"
                     /locus_tag="VF995_11680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEX9344260.1"
                     /translation="MGARGAFTVRVKQELAALRPANPAERRALLAALLRFTATLHIGG
                     TVGPRFTLVLSSRSGGVVRLAFWLLHDGYGVRSDFRVRSQGALAPGSRYELVLAERVE
                     RILTDTGLLDRSGRLASGIPGGLVRGGAAAACFLRGAFLGRGSVSAPTRGPHLEIGTP
                     EERSARDLAVLLVRLGLPASPSARGDDEHRVVVKGGEAIGRALATMGARDAYLAWEDG
                     RIRREVRGEAVRLANADQANLRRSVAAAIGQLALVEAAVARHGWDGLPAEVADVAALR
                     LANPEATLAELGALLDPPRSKGAVAARLKRLEALAEAAGETEGR"
     gene            complement(3274..4224)
                     /gene="yvcK"
                     /locus_tag="VF995_11685"
     CDS             complement(3274..4224)
                     /gene="yvcK"
                     /locus_tag="VF995_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HEX9344261.1"
                     /translation="MSPDIEVAALGGGHGLSATLTALRLLGAEPTAVVAMADDGGSSG
                     RLRRDLGLPPPGDLRMALLALADPAGAASGSEGGTAEAEVAALFGYRFDHGELAGHSL
                     GNLVLAALSELRGGFMEALDTAHRLLHTRGRVLPSTLVPLRLSGQVDGQLVEGQAAIT
                     TARGRVEQVWLDPPDAAALPEAVAAVERADLVLLGPGSTFTSVVSTLLVPELGAAVAA
                     AAKDARVVYLCNLDPQSPLDETSGFPPEAHLAVLLDHCPGLRIPLVLCQRPAEEATAE
                     RETHDFAALGARVLHADLADHRIPGRHDPARLAVALKELG"
     gene            complement(4238..5137)
                     /gene="rapZ"
                     /locus_tag="VF995_11690"
     CDS             complement(4238..5137)
                     /gene="rapZ"
                     /locus_tag="VF995_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008748569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX9344262.1"
                     /translation="MSKGGRTAQTAWPHAVDFVVITGLSGAGRSEAAKVLEDLGYFVI
                     DNLPPSLIGRVAELALAGREHTRVALVVDARGGAFAGDVSELSSALQPLRDQQLTLRI
                     LYLEASDELLVRRFEHNRRRHPVAGERVVDSIAIERDLLRELRADADLVIDTSDLNVH
                     ELREKILAAFADEAAGLLVTVTSFGFKYGLPLDADTVVDVRFLPNPHWIPELRPHTGL
                     DPQVRDYVLGQEATKRFMDRLQELLEVSLPGYVREGKQYLTIAIGCTGGKHRSVVLSE
                     ELRAWLQGKDYRVNVVHRDMERE"
     gene            complement(5134..>5552)
                     /locus_tag="VF995_11695"
     CDS             complement(5134..>5552)
                     /locus_tag="VF995_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9344263.1"
                     /translation="ADRPDLAVISLAKRMEEVYLPGSPDPVVLPRASEALYLLQRVRD
                     EAHRFAIAYHRKLRGRSMTRSALEDIPGVGEARRTDLIRQFGSVRKLGQASVEEIAAV
                     PGFGPRLAATIWQHLHGTPAASPAAAEAPATSKGGP"
BASE COUNT          770 a         1927 c         2114 g          741 t
ORIGIN      
        1 ggtcgacccg gtcggccagg ccgagctggg cgagcgcggc ggcggagtcg ccgccgccga
       61 cgacggtgta ggcgtcgctg gaggcgacgg cctcggcgac cgcccgggtc ccagcggcga
      121 acgggggcca ctcgaacacg cccatgggac cattccagaa caccgtcttg gcgtcccgga
      181 cggcggcggc gaagctggca gccgtctcgg ggccgatgtc gaggccacgc cagcccggct
      241 cgatcccgcc ggcggggacg accttgtggt cggcgtccgc ggcgaaggcg tcggcgacga
      301 ccacgtcggt cggcaacagc acccgcctgc cctcccgccc ggcgcgggcg agcaggtcgc
      361 ggacggcgtc cacctggtcg ggctcgaaca gcgagtcgcc gacgccgtcg ccgttggcgg
      421 cgaggaaggt gaagcacatc ccgccgccga ccaccagggc gtcgacccgg ccgaggaggt
      481 tgtcgaggat ggcgagcttg tcggagacct tgctgccgcc gagcaccgcc acgaacggcg
      541 acgccgggcc ctccagcagg cgcgacagct ctttgacctc gccggcgagc aggaagccgg
      601 cgtaggcggg gaggcgctcg gcaacgccga cggtcgaggc gtgggcgcgg tgcgcggcgc
      661 cgaaggcgtc gtccacgtag acctcggcga ggctggcgag ctggtcgacg aaggtggggt
      721 cgttggcctc ctcgcccggg tcgaagcgga ggttctgcag cagcagcacc tccccgggcc
      781 gcagcgcctc ggcggcgtcg tgggcggcct gcccggcgac gtcgtcaacg aagttgacgt
      841 cgcgtccgag ctgctcggcg agcgcggaga cgaccggcgc cagcgagtac ttctcgaccc
      901 gcttgccttt cggccggccg aggtgggagc agcagaccac gctggcgccg cgggtgagca
      961 gcgaccgcag cgtcgggatg gcggcgcgga tgcgcaggtc gtcggtgacg tgcccctcgt
     1021 cgtcgagggg cacgttgaag tcgtcgcgga ccaggacccg ccggccgctc acctccgcct
     1081 ggtcgacggt gcgaagctcc atcgcgtcgc ttcctcttcc tgtcccctgc cgccgctacc
     1141 gcagcgagcc gccgaccagc tgacagagat ccacgagccg gttggaatag ccccactcgt
     1201 tgtcgtacca gccgaacacc ttggcgaggt tgccgttggc ctgcgtcagg gtcgagtcga
     1261 agatgcagga ggccgggtcg ccgacgatgt cggaggagac gatcaggtcg gtgttgtagc
     1321 gcaggacccc ctggaggtcg ccctcggcgg cgcgcttgaa cgcggcgttg atctcctcga
     1381 cggtgacctc gcggccgagg atcacggcca ggtcggtgag cgagccgtcc accaccggga
     1441 cgcgcagcga gatgccgtcg agcttgccct tgagctccgg gatgaccagg tgggcggcgc
     1501 gggccgcgcc ggtggaggtg gggatcacgt tggcggaggc ggtgcgggcg cggcgcagat
     1561 ccttgtgctg gaagtcgagg gtggactggt cgttggtgaa ggcatggacg gtggtcatga
     1621 agcccttctc gatgccgaac tggtcgagca ggaccttggc cagcgggatc accgagttgg
     1681 tggtgcagga ggcgttggag atgacgtcgt gggtggccgg gtcgtacgtg gcgtcgttga
     1741 cgcccatcac gatggtggcg tcggcgccct tggccggcgc ggagatgatc accttcttgg
     1801 cgcccgcctt gaggtggagg gcggccttgt cccgctcggt gaagaagcca gtggattcga
     1861 ccaccacgtc gacgccgagg tcgccccagg cgagctgggc cgggtcgcgc tcggcgctcg
     1921 ccttgatctg ctggccgtca acagtgatcg aggtgtcgtc gtagctgacc tcggcgttga
     1981 gcttgccgaa ggtggagtcg tacttgagca ggtgggcgag ggtgtggtta tcggtgaggt
     2041 cgttgaccgc cacgacctcg acgtcgatgt cgtgcttgct gcggctggcc gcctcgcgag
     2101 cggcgcggaa gaagatacgt ccaatcctgc caaaaccgtt gatgccgacg cgaatggtca
     2161 tcggatcctc ccatttcaag ccatcgctgg ccatcgatca gatgtgttgg gactgccaaa
     2221 gctcacgcgg gccattcttc cccagggcgg ggaaggccgc cacgcgttgt ccacagcccc
     2281 gccagggtca gccagggtca gccagggtca gcgcccctcg gtctcgcccg ccgcttcggc
     2341 gagcgcctcc agccgtttga gccgggcggc gaccgcgccc ttggagcggg gcgggtcgag
     2401 cagggcgccc agctccgcga gggtcgcctc cgggttcgcg aggcgcagcg cggcgacgtc
     2461 ggcgacctcg gcgggcaggc cgtcccagcc gtggcgggcc acggccgcct cgaccagggc
     2521 gagctggccg atggcggcgg cgacgctgcg gcgcaggttg gcctggtcgg cgttggcgag
     2581 ccgaaccgcc tcgccgcgga cctcacggcg gatgcggccg tcctcccagg cgagataggc
     2641 gtcgcgggcg cccatggtgg ccagtgcccg gccgatcgcc tcaccgccct tcaccaccac
     2701 ccgatgctcg tcgtcgccgc gggcgctggg gctggccggc aggcccagcc ggacgagcag
     2761 gacggccaga tcgcgggcgc tgcgctcctc gggcgtgccg atctccaggt gcggccccct
     2821 ggtcggcgcc gagaccgacc cacggccgag gaaggcgccg cgcaggaagc acgcggccgc
     2881 cgcgccgccg cgcaccagac cgcccgggat cccagaggcg agccgcccgc tccggtcgag
     2941 caggccggtg tcggtgagga tccgctcgac ccgctcggcg agcaccagtt cgtagcgtga
     3001 ccccggcgcg agcgccccct ggctgcggac ccggaagtcc gaccgcaccc cgtacccgtc
     3061 atgcagcagc cagaacgcca gccgcacgac cccacccgag cgcgacgaca gcaccagcgt
     3121 gaaccgtggc ccgaccgtcc cgccgatgtg cagcgtggcg gtgaagcgca gcagcgccgc
     3181 cagcagcgcg cgccgctcgg ccggattcgc tggccgcaac gccgcaagct cctgcttgac
     3241 ccggaccgtg aacgcgccac gggcgcccat tgatcagccc agctctttga gagcgaccgc
     3301 gaggcgggca gggtcgtggc ggccggggat gcggtggtcg gcgaggtcgg cgtgcagcac
     3361 ccgggcgccg agggcggcga agtcgtgagt ctcgcgctcg gcggtggcct cctcggcggg
     3421 ccgctggcac agcaccagcg ggatccgcaa ccccgggcag tggtcgagca gcacggcgag
     3481 atgcgcctcg ggcgggaacc cgctggtctc gtccagcggc gactgcgggt cgaggttgca
     3541 gaggtagacg actcgcgcgt ccttcgcggc ggcggcgacc gcggcgccaa gctccggcac
     3601 cagcagggtc gagaccaccg aggtgaaggt cgacccgggc ccgagcagca ccaggtcggc
     3661 ccgctcgacc gcggccaccg cctcgggcag cgcggcggcg tcgggcgggt cgagccagac
     3721 ctgctcgacg cggccgcggg cggtggtgat cgccgcctgt ccctccacca gctggccgtc
     3781 cacctggccg gagaggcgta gcgggaccag ggtcgagggc agcacccgcc cgcgggtgtg
     3841 cagcagccgg tgggcggtgt cgagcgcctc catgaacccg ccgcgcaact cggagagggc
     3901 ggcgagcacg aggttgccga ggctgtgccc ggccagctcg ccgtggtcga accggtagcc
     3961 gaacaacgcg gcgacctccg cctcggcggt gccgccctcg ctgccgctgg cggcgccggc
     4021 cgggtcggcc agggcgagca gcgccatgcg caggtcgccg ggaggcggca ggccgaggtc
     4081 gcggcgcagg cggccggagg agccgccgtc gtcggccatg gcgaccacgg cggtcggctc
     4141 ggcgccgagc agccgcaggg cggtcagggt cgcggagagg ccgtgcccgc cgcccagcgc
     4201 ggcgacctcg atgtcagggc tcaccgccgg actcacttca ctcgcgttcc atgtcccggt
     4261 gaacgacgtt gacccggtag tccttgccct ggagccaggc gcgcagctcc tcggacagga
     4321 ccacgctgcg gtgcttgccg ccggtgcagc cgatcgcgat ggtgaggtac tgcttgcctt
     4381 cccgcacgta gccggggagc gaaacctcga gcagctcctg cagccggtcc atgaaccgct
     4441 tggtggcctc ctggcccagg acgtagtcgc ggacctgcgg gtcaaggccg gtgtggggac
     4501 gcagctccgg gatccagtgc gggttcggca ggaagcggac gtcgaccacc gtgtcggcgt
     4561 ccagcggcag gccgtacttg aagccgaacg aggtgacggt gaccagcagc ccggcggcct
     4621 cgtcggcgaa ggcggcaaga atcttctcgc gtagctcgtg gacgttgagg tcgctggtgt
     4681 cgatcaccag gtcggcgtcc gcgcgcagct cgcgcagcag gtcccgctcg atcgcgatcg
     4741 agtcgaccac ccgctcgccg gcgaccgggt ggcggcggcg gttgtgctcg aagcggcgca
     4801 ccagcagctc gtcggaggct tccaggtaga ggatccgcag ggtcagctgc tggtcgcgga
     4861 gcggctgcag ggcgctggac agctcggaga cgtcgccggc gaaggcgccg ccgcgcgcgt
     4921 cgaccaccag cgcgacgcgg gtgtgctcgc ggccggccag ggccagctcg gcgacccggc
     4981 cgatcagcga cggcggcagg ttgtcgatca cgaagtagcc gagatcctcc agcaccttgg
     5041 ccgcctcgga acggccggcg ccggagagcc cggtgatcac cacgaaatcg accgcgtgcg
     5101 gccatgccgt ctgtgcggtg cggcctccct tgctcaaggt ccccccttgc tcgtcgccgg
     5161 agcttcggct gcggccggcg aagcggccgg cgtcccgtgc agatgctgcc agatcgtcgc
     5221 ggccaggcgc gggccgaacc cggggaccgc ggcgatctcc tcgacgctgg cctggccgag
     5281 cttgcggacc gagccgaact ggcggatcag gtccgtgcgc cgcgcctccc ccactccagg
     5341 gatgtcttcg agggcgctgc gggtcatcga cctgccgcgc agcttgcgat ggtaggcgat
     5401 ggcgaagcgg tgcgcctcgt cgcggacccg ctgcagcaga tacagcgcct cgctggcgcg
     5461 tggcagcacc accgggtcgg gcgagccggg caggtagacc tcctccatcc gcttggccag
     5521 cgagatcacg gcgaggtcgg gccggtcggc gg
//
